LOCUS       DAOSIO010000001        35206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_1401_length_35206_cov_4.745043, whole genome shotgun sequence.
ACCESSION   DAOSIO010000001 DAOSIO010000000
VERSION     DAOSIO010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 35206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35206
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1401_length_35206_cov_4.745043"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PK590_00005"
     CDS             complement(<1..300)
                     /locus_tag="PK590_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23012.1"
                     /translation="MTKKKIVILEPAGGPWIPFLKNFFSDSFSEVVCAHDRDAILPFF
                     SKSRPAMVFVAPSFLSLPFLQKIKVQWQTNPSFRAYVIGDGTWLRADPVFSASFDF"
     gene            complement(376..795)
                     /locus_tag="PK590_00010"
     CDS             complement(376..795)
                     /locus_tag="PK590_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOG23013.1"
                     /translation="MNFTRFGSSKGFTLTEILIVVVVIAVMASLVIPRFTGQTDKAAA
                     AEAIGIMGAIHRALLQFNDENGAYPANNLQNITDITNTLGIDSSPVRFGWEFSTSGGT
                     VTGTRDGFGTLTLAEDGTWDGTNRYAPNGDLWPHLPQ"
     gene            complement(820..1254)
                     /locus_tag="PK590_00015"
     CDS             complement(820..1254)
                     /locus_tag="PK590_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOG23014.1"
                     /translation="MKTDCFRKKNAGFTLAELLMVIVIIGVLGSIAIPRFFPQKEKAV
                     VAEAVNMMGAIRQGEIAYRLEHAGYLNLPQTGHTWDEIGIDDPNSSGARKFDYAVNNT
                     IITATRLDTAGTQDDGYVNRTITLDVDSGVWGGTHPFHPRNQ"
     gene            complement(1575..2024)
                     /locus_tag="PK590_00020"
     CDS             complement(1575..2024)
                     /locus_tag="PK590_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HOG23015.1"
                     /translation="MARGALVAKNGLKTRGLTLAELLVVIVILGILGSLAMPRLFPQK
                     EKAVVGEAISTLHAIRMGEKSYHADTGNFLTLGALDNNATWNQIGIDNPGSARFTYAV
                     DATSGQVTATRTGNNVPQRYHGTTIVLVLENGSWDEASSTHPLAPKN"
     gene            complement(2140..2553)
                     /locus_tag="PK590_00025"
     CDS             complement(2140..2553)
                     /locus_tag="PK590_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOG23016.1"
                     /translation="MNKKGFTLIEVLIVVVIIAVLASLLLPRMAAQTECAIAAEGLQT
                     LGVIRRAQEQRAELSGNWVAVAANANFPAALGITIGNNTRFNYTCAVNNNIGTCTATR
                     TNDNTRTISVNSNGIVVCGAGYTAVRSDNRTVGCA"
     gene            complement(2678..4000)
                     /locus_tag="PK590_00030"
     CDS             complement(2678..4000)
                     /locus_tag="PK590_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOG23017.1"
                     /translation="MNKRSFQVGKEETLEQFECEVRQRDNPAELKKMTIQARSKDECA
                     RRLLDQGFLVVSIKQSGQQESSGVSGLFAGYLKGRGSDGASKGGRTLFSKGVTTRELI
                     FFAVQLSTLLKAGIPLIRSLEIIRKGTPNPYFQSVIQKLSQSVAGGSPLGVGLKNQGK
                     VFPWVWVNLVEVGEATGRLPESLEEIAKYQESAARIKAKILTAFFYPTILAVAVVAAL
                     AFLLIFIVPKFAEIFAQQNMTLPAITQVVISLSNIVKNQAYLLLLAFIPFIILGYLVK
                     TSPQVRLSFDLFRLNVPLFGPLLVQITVVRFSRALSTLLKSGVQILQALEICGRLVEN
                     AYMEAGIKEVIKRVRTGQGLGAQLEARKLFPVFMTQLVTVGEETGQIDKFLDLLGVYY
                     EERVDAFLARLTSMLEPIMLVFMGGVIGVIVVSMFLPIIELSTRGGVG"
     gene            complement(3993..5687)
                     /locus_tag="PK590_00035"
     CDS             complement(3993..5687)
                     /locus_tag="PK590_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOG23018.1"
                     /translation="MRLNSMLGELLIEKKLISPNQLERALSDQKETKEPLGQVLIRLG
                     YISEGDLRMTLSEQLGISFLAPDQLTPRGDQRLKEIIPKEFALEHKILPLSREERALK
                     IAASKMPDLIFLDNLRKMTGLDIVTVLSSELDIKRAIELYYSATDFQEIVSSSEVDEA
                     SQGLEPSKEETKLDLESAMAEAGQAPVVRLVDVMLKKALDDRASDIHIEPFHEKMNVR
                     YRVDGALYELPPPPKELHSAVVSRIKILSKLDIAEKRIPQDGSFSISYKGRRVDVRVS
                     TIPIVSGEKVVMRILDKRMDLLNLKALGFEPEQQQLFENALVRPYGLIFITGPTGSGK
                     STTLYAGLNRIHSSDINIVTIEDPVEYQITGVNQVQVKPDIGLTFASGLRAFLRQDPD
                     VILVGETRDLETAEICIRAALTGHLVLSTLHTNDAATAVTRLIDIGIQPYLVSGSLAL
                     VAAQRLVRVLCPDCKEPYKPSDKERSEYGIHADTIYKAKGCSKCRQIGYWGRAAIYEV
                     ILIDEDFRRLIVKDANLDEIRRVQKEKKYETLFQNGMKKVERGLTTIDEILSVAYE"
     gene            complement(5746..7509)
                     /locus_tag="PK590_00040"
     CDS             complement(5746..7509)
                     /locus_tag="PK590_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOG23019.1"
                     /translation="MSENKFKIIVAVTLTVLFCGTMPVYALGEEDQGAEPKVQVERTE
                     AGKFFLDFKAAPLINVLNVIASLSGINFVAGKEVAEREVNMTLDNVSLEDALQAISYG
                     SNVSYDFLPERSIYLFRASADSPERPPLMTRVFKLYYVRVTKALEIEADSSSSSSSSS
                     SSSSGSGTGSSSGGGLVTLSQDQEEIDLESSAIYKAVEKILSDRGSVSVDDRTNSLVV
                     TDSEDRLKIIGDAIAQLDRPLDQVLINVLLVETYEDLDRALGVQWGDTDGLLGTVTGA
                     VQNTDWPFRSGGSTIETKSFGDFFSNVGNTFKDSANQFDPRINSGPITTPGTRDFSSF
                     QISVRALEEANKVKILAKPKILVLDNHPALIKIATNAAIGTTTVTTAEGAGTTSTGLE
                     RAEVGTILRVTPLINTRDQVTMTVEPTFATVDNSSITLAQPSGDTTVRTARTTLMVND
                     GQTIALGGLLFSNQSKGERKVPFFGNLPVVGKALFTNTTRTIEDRELILFLSPYIIRD
                     PSELMSKKVPDKRIRYDDEKAPFYQVKKKEWYKRLEEGEEPPIDFESYFDVRKRLMAA
                     TMDTLDEKIAKSDQTALSYET"
     gene            complement(7580..8101)
                     /locus_tag="PK590_00045"
     CDS             complement(7580..8101)
                     /locus_tag="PK590_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23020.1"
                     /translation="MKRIVILGLFLVALNANPFQLYADEAEADLQEDQVDLEVDLQEG
                     QVDLEVSKLRQERQEQIKDLQDDIRDPFTPFLPPKVEDQAVDQVAGETVEVRLYGIGL
                     GSESAYAILNGDVYYEGEEGDGIKLLKVRKNEVEIEMGGVRSTLYILSDDELKRLGQR
                     RERREKARQSVVA"
     gene            complement(8098..8649)
                     /gene="pilO"
                     /locus_tag="PK590_00050"
     CDS             complement(8098..8649)
                     /gene="pilO"
                     /locus_tag="PK590_00050"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOG23021.1"
                     /translation="MTGFVIKERYKEILMVAALGVACAFLGFFVLIQPALRDINWLRQ
                     EIRTAEEKIRLYQETVRLGEKIEKSETVLAETSERSMLIARISDISGKNGVEVQGINP
                     KIVPADKYEKLLLEVDLKGSFLGILKLLQDLQSIHPVLDIPEISLTRSKLLMEETKDS
                     TVNALQGKLVIVTYLKQKKAVGS"
     gene            complement(8664..10181)
                     /gene="pilM"
                     /locus_tag="PK590_00055"
     CDS             complement(8664..10181)
                     /gene="pilM"
                     /locus_tag="PK590_00055"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOG23022.1"
                     /translation="MSKGVGVYIGRDNVVAVSAVSTVKGPQVKAYAIEPVNPGGDGPA
                     VGKEAHKLRKLSPESRAIRKALETIKEPGAFVTASVSPFHIVTRHFMMPAVPKKEKDD
                     AVRFEASRYIPFKLSESVLDYHEQITHRNVYSITATAIKKEVLQQALSDLRGASAKVL
                     MIEPVHSAVGRVFASLNMFQKVKTCGFVTLQSDGNVNLTFVSKGTVYLSRDFLLSEDQ
                     EENKSHFFDELKASLDYFYKLTGGEAVTQIFVAGHGDLKFWVEHLEHAFNYAMRFDLA
                     TFPEGTPFSADIMDSIFIAYGLALRSAGYSSLMGEPALLPPAERRMKPKSFYSLLGGC
                     ALGVMVLFLLVRLVVFQPYVGKLTHEYQKVLGPGSGVDSSVVEMSVESLDSRKVKLAS
                     QAKLLADFEKKKSLPGELLIALGRNTPAVIRIDYISIENTTGGKGKLARNRMTMRGGC
                     VTGNAEQETAAIDAWIQQLQGEGAFKSRFSEFKLEEVKREREMDQVMTQFKVVGE"
     gene            complement(10213..11214)
                     /locus_tag="PK590_00060"
     CDS             complement(10213..11214)
                     /locus_tag="PK590_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23023.1"
                     /translation="MSETQPDNPQGVIEQIETSSLANSNRKAPRSPWRVLPNLVKTGS
                     GFLRRRDLHKIRKHLQAEENLFQSKIPLDPSTSKSDFNVEAESQPLRKEVSDFHERKP
                     KRKSVWRRVWPLIPWLVAGVSLYLLGQGANRETALTSDAGLGDEGQAAWESILLDQEK
                     TIRRLKEQLMDLRGNLQENREDAGGSRLDSKEYRDEVFQLALRYQNEIDALQQTLVER
                     ETVVRDLRAQLTTTQRDAEQYKELASLVVPPDPKAPEAISGKVITVNSRYAFIVVDVG
                     SRHGVCTGQGVQFFKNGKVITRGKVEQVYPGVAGVSLFDALGFSEIIEGDHVIVERG"
     gene            complement(11330..11890)
                     /locus_tag="PK590_00065"
     CDS             complement(11330..11890)
                     /locus_tag="PK590_00065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23024.1"
                     /translation="MTKAKKIKKAARSKTSHSVTPDLVTAMMKLVERLGALEQKMDQV
                     SGRVSNLPSEIRQVIQGFQHSSVSHPSQLSVHPGKSFHPQNPRSLYQAVCADCLKTCE
                     VPFKPSGDRPVYCPECWAIRKAGHRPKDIASGVSVPSHLKQRGTVSTKEIQAPVATPK
                     IAASVKKTPVKKKSKSAVAKKTKKKK"
     gene            complement(11918..12514)
                     /gene="thrH"
                     /locus_tag="PK590_00070"
     CDS             complement(11918..12514)
                     /gene="thrH"
                     /locus_tag="PK590_00070"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HOG23025.1"
                     /translation="MICCLDLEGVLMPEVWIHVAKRTGVKELEITTRDEPDYDKLMKY
                     RIGILKKEKIRLRDIQKVIRAMPPIPGAKGFLKRLKERYQVIILSDTFYEFAGPMMRK
                     LDQPTLFCNWLKTDSDGFISGYVLRQRNGKERAVRGLKSLGFKVIAAGDSYNDVTMLK
                     AADFGAFFRPPSRIAKEFPQFPVTRTYGELLATFLKVN"
     gene            complement(12678..13445)
                     /locus_tag="PK590_00075"
     CDS             complement(12678..13445)
                     /locus_tag="PK590_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23026.1"
                     /translation="MTEPLWAAFKVELDDDEMACLQKIIYLNECSSNPEKLVIWPADE
                     DFASLGIGHAIWYPRGRRGPFRETFPELLLFMKRHGQTLPEWLEKCEDLPWDDRDSFL
                     RDSQSERMKSLRDFLLATRDLQTQFIVERVRKTLPDILSAVSDEERPLIEAKLGRVLG
                     APYGPLAVIDYINFKGEGVLETERSQGEGWGLLQVLRAMGETKEEDEILPEFVRAAKA
                     VLDRRVEISPPGSKETDRIGGWKFRVQRYLMPPCEAK"
     gene            complement(13594..14463)
                     /gene="otsB"
                     /locus_tag="PK590_00080"
     CDS             complement(13594..14463)
                     /gene="otsB"
                     /locus_tag="PK590_00080"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HOG23027.1"
                     /translation="MTLRALDDKTIHVVASSPNLLICLDYDGTLTPIVSRPFLARLHP
                     RARIVLKKLSKMPGVKIVIMSGRALGDLEKQVGLKGLGYVGNHGLERNLGGHPEIDSH
                     AIQLRGFILKLTDALKRAMKNVPAVLIENKTYSATVHYRNLSPSAVAKARRIFRDVWS
                     NFSAKIFFKIRPGKKVWEIRPRRGCSDKGRALQLILRSLTPAQRKGLTTVCVGDDKTD
                     EDAFAVLRRSDVAIYVGESQRTMARYRLRETGAVLDLLEKIKSIRQKASRVQSGGKPL
                     IKSTGRSKLGRKP"
     gene            complement(14476..16695)
                     /locus_tag="PK590_00085"
     CDS             complement(14476..16695)
                     /locus_tag="PK590_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HOG23028.1"
                     /translation="MKITFRLILSLLIATVIVVAGFSYFEAKSEEKRLNEELRLRATV
                     LAKTFKEAIETLLERTDQPSRIQKFIEKFQGHTRLIGLVVDMKDGQRIAFPPHLSRED
                     WFKKESMKVMDENLPLDLNSHWDGRKVNYHAVPLIRDGQVVGALGLIHDRTFIVLQIR
                     QFWKNSALTFSILAGILSIVSLLVIRWNVTGPISRIASMIHKNLGMESAASRGIQQQG
                     EIEKLLYGVVHMAASLKAARLDLVEQSRLANERGSIWTKERLKDYLKTKFRDKQFYVV
                     SNREPYIHRRNGNGIECITPASGVVTALDPVMQATGGMWIAHGAGNADQESVDAENKV
                     RVPPWKPSFTLKRVWLSPEEEQGYYYGFSNEGIWPLCHISHVRPVFRMEDWNYYKEVN
                     RKFANAILEEFGDEPPIVLVQDYHFALLPRMIKERRPDAKVALFWHIPWPNAEAFGIC
                     PWQEEILEGMLGSDMIGFHTQFHCNNFLDTVDRALESKINWTNFEVTRGGKKSTVRSF
                     PVSVSGAPNVAVQETDRDPLEQLRQDYGLKGKKIGIGVDRIDYTKGLLERFWAIERVL
                     EKYPYLQGELVFVELGAPSRTLIGSYQSHLKEVEKLIEKINQRFQKGHYRPILFLEGH
                     HGQKRIFDFYRLADFCLVSSLHDGMNLVAKEFVSAREDEDGVLILSRFAGASYELSSA
                     LLVNPYGIEGTAEAIHQALTMLPEERKERMRKMRSIVKEHNIYRWAADMITELVRSF"
     gene            complement(16720..17595)
                     /locus_tag="PK590_00090"
     CDS             complement(16720..17595)
                     /locus_tag="PK590_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOG23029.1"
                     /translation="MDKSLTMEKIMETPWVTQTLKAVLILVVGWVLVLLLRLALEKFE
                     KAISKTDAIRESGDVLRMKTFSHLLKWIGATLIWLCVIYMLLSSWGINVAPLLAGAGV
                     VGLAFGFGGQYLIRDIINGIFILVEGQFRINDVVQIEGLGGVVEAVNLRHTRLRDLEG
                     RVIYIPNGEIKTVINFTQEYSQALLNVGVAYHTDVDHAIRVIKELGAEMREDGYFKHL
                     ILNDLEMLGVDDFSDSQITLKFRMKTLPIKQWEVAREFRRRLKARFDQEKIQIAFPHR
                     LLYWGTGQNPDKRDA"
     gene            complement(17752..19017)
                     /locus_tag="PK590_00095"
     CDS             complement(17752..19017)
                     /locus_tag="PK590_00095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG23030.1"
                     /translation="MRALIRKKTKLLIFGILATFFSAPGQVYLISFFIPPMCEELGLS
                     QTAISGLFFVGTLSSAFFLPFLGFRLDHERPIRFVTVAGVLLLAGCLFLATAQAPWML
                     LAGFFLVRNFGQGGLAMASQTMMARLKKEHRGQALGIANLGYPLSESLLPGLISTVIT
                     VFGWREGWWALAAMILLIFLPMSRYLIRNEPQPGRAAPDIREDLNAPDPKDSSWPVSR
                     VMKDRRFHLLLLPILVTPSVLTALFFHHAAVIRWKNWDPLLLPAGFVVYAICRAILSF
                     FSGPLTDRFSARKIFALNLIPLTLGILFLWLAKNPFWIFFYFAGSGITMGYGMTVGNA
                     LYAELYGTRHLGSIRGMVSTAIVFSTATAPFLMGICLDAGISLSKILGVIVFLSSAGI
                     ILTYLALKPERSSLGEIAARSLGKEEYKQ"
     gene            complement(19021..19800)
                     /gene="thiD"
                     /locus_tag="PK590_00100"
     CDS             complement(19021..19800)
                     /gene="thiD"
                     /locus_tag="PK590_00100"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOG23031.1"
                     /translation="MIPTALTIAGSDPSGGAGLQADLKTFHQRRVYGMAVVSLLTVQN
                     TRGVRAVHPLKPAQVISQLETVLEDICPRAAKTGALGNEGLIRAIARKAETFRFPLVI
                     DPVMVSKHGARLLASSSVEALKKSLIPHAALVTPNIPEAEILAGIRIGNLSDMKKASR
                     RILELGPRAVLLKGGHLKGASIDVFCDGKRMVLLKAPRIRTRHTHGTGCTYSAAITAE
                     LAKGRELLAAIRIAKRFVTKAIRTAPGLGRGAGPVNHFVKG"
     gene            complement(19863..20357)
                     /locus_tag="PK590_00105"
     CDS             complement(19863..20357)
                     /locus_tag="PK590_00105"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG23032.1"
                     /translation="MRFRKFLMCFLLSWLSVSSVSCAAEVDETVTPVEESSSAASEEL
                     TGDWTTGWYSDAEGYERAFADYERMRRPMAVYINVKWCPYCRVFEKKILSDPRVRQFM
                     RDVIKVKINPESGRREYAIAARYRVMGYPSFFVHPPNQDKTIQLYAGVSPEQFIEIFK
                     KTLK"
     gene            complement(20418..20882)
                     /locus_tag="PK590_00110"
     CDS             complement(20418..20882)
                     /locus_tag="PK590_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23033.1"
                     /translation="MNMKNKWIALVLSFFVPGMGQIYLGDRTKGLTLLCVSAGLAYAL
                     VTSRSLLNLIFLAPICLFVMVPSAVDAYQVAMGKPRKFTGDSVPYVVFMLLVVGPFAV
                     PLLWQSSKFSRFMKIAWTILVILIAIAIILFLRDVASVMDSLLGQDSPAFSF"
     gene            complement(20884..22836)
                     /locus_tag="PK590_00115"
     CDS             complement(20884..22836)
                     /locus_tag="PK590_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOG23034.1"
                     /translation="MGLKIISLMKQVPLPTEMRMGADGLMDRTKAKSMINADCAFGLE
                     QGLQLKKHAPDAELIVVSMGPPSFEQSLKKALAMGYDRAMLLSDRKLGGSDTFATGYA
                     ISSLIKKLGFERGCKDPFIIFSGRQTTDGDTAHVPSQVAENLDIPQATFVEKVEFLDD
                     HVKVRRIIEGGYQVLKLPIPCLISIAPTATPARHPSLAGAVRARDTKIEVWSLDQTGA
                     DSTVVGLTGSPTLVSKVVDIQKDRPPVNMISGHTGRELTDGLVSAIEDLKNRVADESS
                     RSAPASGANTAVAVEPDSGFPKVDFRNGARGVMTWVEMHGQTPARSSLEILSQARKLA
                     DQLGTKVRSVMIGHDIKGAAREIIEHGADEVVIVDDPRLKEYTILPTTTALSQIIEHD
                     RPEIALFGATTSGRELAPRLASRVRAGVTADCTSLEVGDYIHRIKKAVFYPVLQSIRP
                     TYGESKLATIIGFWCPQMATARAGTFKILPRDPSRKGTVTEFKPVFKDTDFKVEVLET
                     RRESGGGDNLFVADIVISGGRPAGDCDEFRLIQDLVEALREQGINADWGASRHAVDNG
                     YAPYARQIGQTGKTVRPKVYLAVAISGAIQHLAGMKESGKIIAINQDPQAAIFRHADF
                     GLVRDYREVLPELIEKVKTGFTFGLR"
     gene            complement(22908..24587)
                     /locus_tag="PK590_00120"
     CDS             complement(22908..24587)
                     /locus_tag="PK590_00120"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HOG23035.1"
                     /translation="MIENNSVKTDVLIVGGGVAGLAFSIHLADLVARHNESATRKLPI
                     NILLVEKGASLGSHSLSGAVVNPSTLKALLPGVEIKDLPFETPVTRDEFYRLTSKTAI
                     KMPFAPPFMSNKGNFVTSAGKIARYLGEIAEKKGVQVYPGFSVEELLYENGRVVGAKM
                     IDTGVSKEGKPMDNYQAGTKVLARLVILAEGSRGSLAKKFIQKHKLQGLNPQVYSTGV
                     KELWEVPEGAFEPGRVIHTLGYPFSFNHFGGGFIYGMTKRTVALGIAAALDYKDPTFD
                     PHAALQLYKKHPRIAKILEKGRILKYGAKTIPEGGFYSLPKLFHDSVMIVGDSAGFLS
                     MPGLKGIHLAIESGMLSAKAAFEAFLKNDFSSEQLKRYEDLFRESPGYKDLYRVRNFR
                     AGFANGMILGAFHFGTQIVTGGRGITPSGKMKIHEDAACYTPLSDVKRPFNKKYEKDL
                     SYDKKLTYDKVTDVFYSGSKHDEEQPCHCHVHDLNLCRDVCIPKYGAPCQYFCPADVY
                     EVVTDPKTGKKDIHLHPANCVHCKTCDIKDPFGNVEWAVPYGGDGPEYDNL"
     gene            complement(24698..25807)
                     /locus_tag="PK590_00125"
     CDS             complement(24698..25807)
                     /locus_tag="PK590_00125"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOG23036.1"
                     /translation="MTKGLGTVETKFHVFPEGLKLESGKTLAPLTLAYETYGELNSEK
                     SNTILVMHAFTGDAHAAGSHKGEKAPGWWDFMIGPGRALNTEKYFVICANVIAGCKGS
                     TGPSSTNPKTGKPYALEFPAVTLADMVHAQVMLIDQLGIRKVYAVIGGSMGGMLALQW
                     AALYPDRIECVIPIAATLKHSPQQIAFNEVGRQAIMADPDWQKGNYYGKNLPEHGLAV
                     ARMLGHITYMSNESMEKKFARRVKEDNGGFQFSPDFEVEGYLQYRGQSFVKRFDANSY
                     LYITKAMDYFDLSGDKLFEKGSLDHARFLVIAFKSDWLYPSVQSHDMVRQLKMHNIDA
                     TYCEIDAGYGHDSFLIESEGIKQAHLIKHFLAKRK"
     gene            complement(25804..26739)
                     /gene="cysK"
                     /locus_tag="PK590_00130"
     CDS             complement(25804..26739)
                     /gene="cysK"
                     /locus_tag="PK590_00130"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017200618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOG23037.1"
                     /translation="MARILEDVTQSVGDTPLVRLNRLTAGLRADVLVKLETRNPMSSI
                     KDRIGVAMIDAAEKNGQIKPGGTIVEPTSGNTGIALAFVAAARGYKLILTMPDTMSVE
                     RRQLLKILGAEVVLTEGSKGMRGAVEKASEIQSNTPGSFMPQQFNNPANAEAHRKTTA
                     DEIWRDTEGKVDFFVAGVGTGGTLTGVGEELKKRKPSARIIAVEPADSAVLSGGKPGL
                     HKIQGIGAGFIPRVLNTRIYDEIIKVKNEDAGETARQLAKQEGILGGISTGGNVWAAL
                     EIARRPESKGKTIVTVICDTGERYLSTWLFEDCAR"
     gene            complement(26828..28120)
                     /locus_tag="PK590_00135"
     CDS             complement(26828..28120)
                     /locus_tag="PK590_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOG23038.1"
                     /translation="MSQREKKFKSETIVLHGGQTVDPVTGARAVPIYQTTSYQFKSTD
                     HAADLFGLKETGNIYTRLMNPTTDVLEKRIAQLDGGVGALAVSSGQSAIALALLNIAQ
                     AGDEIVSADNLYGGTYNLLHYTFARFGIRVKFVKSNDLQGFQKAITPRTKAIYAESIG
                     NPKLDVADLEGLAGVARKNGIPLVLDNTVSPYLLKPFDHGVDVTVYSATKFIGGHGTS
                     LGGLIVDSGKFDWTNGKFPLIADPDPSYHGINFVDHLKPFGNIAYIVKARLTVLRDLG
                     CALSPFNAFLFLQGLETLHLRMPKHSENALCVARHLEKNPKVAWVHYPGLASSSEKAR
                     SKKYLPNGAGAIIGFGIKGGLKAGKKFIDSLKLISHLANIGDAKTLAIHPASTTHQQL
                     SDEERAATGVTPDFIRLSIGLEHIDDILADIDQALQKA"
     gene            complement(28167..28619)
                     /locus_tag="PK590_00140"
     CDS             complement(28167..28619)
                     /locus_tag="PK590_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOG23039.1"
                     /translation="MISNKTKYALLAMLHLAARFSSGEPVLAAEIAERESIPKKFLEV
                     ILLELKNKGLLLSRKGRGGGYLLARQPGEITFGDVIRVFEGSMAPVVCVEPGRVAQCP
                     ECQGTHSCAIRGVMRELYSAMVGVLDGASLEQASEKKIRSECANMYFI"
     gene            28877..29116
                     /locus_tag="PK590_00145"
     CDS             28877..29116
                     /locus_tag="PK590_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23040.1"
                     /translation="MGSEASWEYVVRDFLEVKCALKEQEQEYLLDFLKTKGIREEPVR
                     IYPAFSIADCLVVVTDGAKAKRRWFYHRGKKKIEE"
     gene            complement(29164..29934)
                     /locus_tag="PK590_00150"
     CDS             complement(29164..29934)
                     /locus_tag="PK590_00150"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOG23041.1"
                     /translation="MRIAHALATAGLGSRRKCEVFVTNGAVTVNGEIVRDLGRQVDFS
                     KDTIAYRGKILECSPNVYYILNKPVGYVVTAQDAYAKKTVYDLLPRQLVNATRQGSRG
                     KVRVFPVGRLDKDSMGILLLTNDGELSHRLLHPRYHVEKWYEVTLDRAWRPEDSGKVI
                     NGFHAFDGHLKLEQIRPVSKRRLMVQLREGKKRQIRRVFQRFDYEVVTLVRISFGPVK
                     LSNLRLGEGRFLTSFESRELREAVGLSSNAGNVGHDAR"
     gene            complement(29931..30536)
                     /gene="trmB"
                     /locus_tag="PK590_00155"
     CDS             complement(29931..30536)
                     /gene="trmB"
                     /locus_tag="PK590_00155"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOG23042.1"
                     /translation="MSWAFVKGTYPPISLRELFGNDNPVEVEIGCGKGSFLVARAEEN
                     PGINFIGIDRIAKYMKRRKMRVEREEARNIQFIQAESRKFLEEALRPLSVSVFHMYFP
                     DPWPKRRHRARRTFSEDFLKLIHSRLSPGGLFEVATDDLDYFTEMKKGIAATRELWDN
                     VRETVNERIFGGTMKTNYEIKWAAEGRALYYAELAKGTKGS"
     gene            complement(30518..31543)
                     /locus_tag="PK590_00160"
     CDS             complement(30518..31543)
                     /locus_tag="PK590_00160"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23043.1"
                     /translation="MTPLCSLFGSCGGCKYQDISYEEELSIKEKALRDLLTGTIEGLD
                     PLVFEPLVPSPELYYYRNRLDLTFRRDREGRWRMGFMLENTHKLEDVTVCPIARKEIN
                     DFLPKLREEATVKAVAGEYRNANLTVRVGEEGKVAWGGIGRRSLRMDPENYFSVTIRG
                     RRIFYSLDTFFQANLSILGKVADTISAGSVWKPETVFLDLYSGVGLFGLALADLAKEV
                     VMIESGYDSVRLAKHNIAYHKLEHVKVFEGQVEKLLPEILSALRGRHCVALIDPPRSG
                     LYPSAIQTLNQSDGISALFYLSCSPESLSRDLASLKDRWKIERIIPFDFFPRTRHLET
                     LVCLGRL"
     gene            complement(31788..32429)
                     /gene="leuD"
                     /locus_tag="PK590_00165"
     CDS             complement(31788..32429)
                     /gene="leuD"
                     /locus_tag="PK590_00165"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012824574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOG23044.1"
                     /translation="MKAFENFRGKVAPLDRANVDTDAIIPKQFLKSIRRTGFGESLFY
                     DWRYLPDGKPDPKFVLNQAPYRNATVLVARNNFGCGSSREHAVWAVCQYGIRVVIAPW
                     VDDGDHRVPAFADIFRNNSVKNGLLTIELSSREVDEIFKAIADEAGLEASIHLQDQTV
                     TLHARPQDLKFHFEIDPAIKERFLRGLDDIGITLEHEPSIRAFEKSRQTWSCL"
     gene            complement(32506..33903)
                     /gene="leuC"
                     /locus_tag="PK590_00170"
     CDS             complement(32506..33903)
                     /gene="leuC"
                     /locus_tag="PK590_00170"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOG23045.1"
                     /translation="MPKTLFEKIWEKHVVTTLPDGTAVLFIDRHLVHEVTSAQAFEGL
                     RLAGRKVRHPELTFATMDHNVPTNDRFNIRDLIAKEQMEAITRNCKEFSITLYDLHSD
                     RQGVIHIIGPELGITLPGTTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQCLLQK
                     KPKTFKVEFTGRVPSSVTAKDMILKLIGMIGTAGGTGYAIEFCGEAIRALSMEGRMTV
                     CNMAIEAGARSGMIAPDETTFRYFTETSRPYAPKGEDLEAAIACWKTLPTDPGAKFDR
                     EFVIDASRIAPQVTWGTSPGMVTDVDGVVPAPEQVQGYSRADVEKAIHYMGLKSGMKM
                     TDIRIDTVFIGSCTNGRIEDLRAAANYLKGRKVAQGVRVLVVPGSQKVKAQAESEGLD
                     VIFKEAGCEWRESGCSMCLAMNPDQLRPGERCASTSNRNFEGRQGKDGRTHLVGPEMA
                     AAAAVAGHFVDIRKT"
     gene            34227..35057
                     /locus_tag="PK590_00175"
     CDS             34227..35057
                     /locus_tag="PK590_00175"
                     /inference="COORDINATES: protein motif:HMM:NF016852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HOG23046.1"
                     /translation="MQDISLELKRTQIEILKVIDRICRDHDIKYSLFYGSLLGAVRHQ
                     GIIPWDDDLDICMSREDYNRFTRIWNNVNPQGYILQNKENSRAFAEAFTKIRKDHTCF
                     LQKGERANAYHNGIFVDIFPIDRLPNGKMKRFAFKLNCKLYQLFTRELVPSKINTIAG
                     AIFSILLLCTPHFCRMGLREKLLKKITKYNSNTALETVNITTKAFLETPFSSTMLNNY
                     TELDFSNEKFMCYEEWDDCLTRIYGDYMQLPPEEERVWKHHPIIIDFSHNYDELENAK
                     "
     gene            35047..35196
                     /locus_tag="PK590_00180"
     CDS             35047..35196
                     /locus_tag="PK590_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23047.1"
                     /translation="MQNKQTYPRIAIMGFMSRGGAKTGERLTKQEIWETNISLRMANS
                     TQINL"
BASE COUNT         8719 a         9886 c         8179 g         8422 t
ORIGIN      
        1 aaaatcgaat gaagcgctaa aaaccgggtc cgcccgaagc cacgtcccgt ccccgatcac
       61 ataagcccgg aacgaaggat tagtctgcca ttgaaccttg attttttgaa gaaatggcag
      121 actcaaaaaa gagggcgcca caaaaaccat cgcgggacgg gattttgaaa aaaacggaag
      181 aatagcatct cggtcgtggg cgcaaacaac ctcggaaaaa ctgtccgaga agaaattttt
      241 taggaaagga atccaggggc cgccggccgg ctccagaatc acaatctttt ttttcgtcat
      301 gtccggcaat tcatccctaa gccttgtaga gcagaactat caggtccacc acacccttct
      361 gtatccggca atctctcact ggggcaaatg gggccacaaa tctccattcg gcgcgtatcg
      421 attcgtaccg tcccatgtcc cgtcttccgc taaagttaga gtcccaaagc catcccgtgt
      481 cccggtgaca gttcctccac tcgtcgaaaa ctcccagcca aacctcacag gactcgaatc
      541 gataccaaga gtgttggtaa tatcagtaat attctgaagg ttattagcag gatacgctcc
      601 attttcatca ttgaattgga gaagggcacg gtgaatggct cccataatcc cgatcgcttc
      661 cgcggcggcc gccttgtccg tttgccccgt aaagcgcggt atcacaagag atgccatcac
      721 ggcaatcaca acgacaacta tcaaaatttc cgtaagcgta aatcctttgg aagatccgaa
      781 acgcgtaaaa ttcatgtcgc cacctcttta tttttaaagc tattgatttc gggggtggaa
      841 agggtgagtc ccgccccata cgccgctatc tacatccaga gtaatggtcc tgttaacata
      901 cccgtcgtcc tgcgtaccag ctgtatcaag ccgggtcgcc gtaataatgg tattatttac
      961 tgcatagtca aacttgcgag cgccactact attgggatca tcaatcccga tctcatccca
     1021 ggtgtgacca gtctggggaa gatttaaata acccgcatgc tccagccggt aggcaatctc
     1081 gccttgccgg atcgcgccca tcatgttgac ggcttcggcg acaacggcct tttctttttg
     1141 cgggaaaaac cgggggatcg cgatggatcc caacacgcca ataataacaa tcaccatcaa
     1201 aagctcggca agcgtaaaac ccgcgttttt cttcctgaaa cagtccgtct tcatgattgt
     1261 ctcctcctct aatccacccg ttcttatccc gtcactccaa aaaacgtccc acccctgtcg
     1321 tcctcgcttc gtcattcccc aaaacatccc ttcctcgtca ttctgaggac gaagtccgaa
     1381 gaatctcaaa ccaagatcct tcgcttcgct caggatgacg gcttgcctag gataacgctt
     1441 cgctcaggat gacgacttgc ttaagataac gcttcgctca agacgccaaa ttgcttagga
     1501 taacgattcg ttcccaacgg gtcccctctg ggtcgcggac aggatggcaa catcccgaaa
     1561 gcctgaaaaa tcgtttagtt cttcggcgca agcggatggg tcgaactcgc ctcatcccaa
     1621 ctcccgtttt caagaaccag gacaatggtc gtaccgtgat atctttgagg gacattgttc
     1681 cctgtacgcg tggccgtaac ctgtccgctt gtggcgtcga cagcgtaggt aaaccgagca
     1741 gaaccgggat tatcgattcc gatctgattc caggtggcat tattatccaa agcgccaagg
     1801 gttaaaaaat tacccgtgtc cgcgtgatac gatttttccc ccatccgaat ggcatgaagc
     1861 gtactgatcg cttcccccac gaccgccttt tccttctggg gaaaaagtct cggcatcgcc
     1921 agcgaaccca atatcccgag aatcactatc accaccaaaa gctcggccag cgtcaaacct
     1981 cttgttttta ggccgttctt cgccacaagc gctcctcttg ccatccagcg taaagtccgg
     2041 ctccaccgtc agggtagagc cggacattca gaaaccctca ctattcttaa acaccaaaac
     2101 catccgcgtt tcaatcgatc tctaaccttc tcccgcgaat caggcgcaac caacagtcct
     2161 attgtcagac cgcacggccg tatagccagc accacaaaca acaataccgt tgctgttaac
     2221 tgatatcgtc ctggtgttat catttgttcg cgttgccgta caggtgccaa tgttgttatt
     2281 cacagcgcat gtatagttga atcttgtgtt atttccgatg gtaatgccca gcgcggctgg
     2341 gaaattagcg tttgcggcaa ctgctaccca gttgccgcta agctccgctc gttgttcttg
     2401 ggcacgccga atgactccaa gggtctggag cccttcagca gcaatagcac actcagtctg
     2461 agccgccatt ctgggtagca aaaggctcgc cagcaccgcg ataataacca ccacgatcag
     2521 cacttcaata agggtaaaac cttttttatt catggccttt ctcctttttt gatttttaac
     2581 cacatcagga acgtcccctg cactagcaag aatagcaagg aacctcgcga aacacaacgg
     2641 tctctttcgt gtgttcccgc aaactgcttc acccgcttta tcccaccccc cccctcgttg
     2701 agagttcaat aatcgggaga aacatcgaga ccacaatcac gccgatcact cctcccataa
     2761 aaacaagcat gatcggttcc agcatgctgg taagacgcgc caaaaacgcg tccacgcgtt
     2821 cttcgtaata gactcccaac agatccagaa atttatcgat ctgccccgtc tcctcaccga
     2881 cggtcacaag ctgggtcatg aaaacaggga aaagttttcg cgcttcaagt tgcgcgccaa
     2941 gcccctggcc ggttcggacc cgcttgatta cttccttgat ccccgcctcc atgtaggcgt
     3001 tttcaacaag ccgtccgcag atttcaagcg cctggaggat ttgaacgcct gattttaaaa
     3061 gcgtgctgag cgcgcgggaa aaacggacaa cggtgatctg gacaagaagg gggccaaaca
     3121 gcgggacatt aagtctgaat aaatcaaatg aaagacgaac ctggggcgac gttttgacga
     3181 gatatcccaa aattataaaa gggataaatg ccagcaaaag aagatacgcc tgatttttca
     3241 cgatattgct caagctgatg accacctggg ttatcgcggg caaagtcatg ttttgctggg
     3301 caaaaatttc cgcgaacttg gggacaataa aaataagcaa aaaggccagg gccgcgacca
     3361 cggcgacggc taaaatcgtg ggataaaaaa aggcggtcaa aattttggct ttaatccgag
     3421 cggctgattc ctggtatttg gcgatctctt caagggactc gggaagcctg ccagtcgcct
     3481 cccccacctc gacaaggttt acccaaaccc acgggaaaac ctttccctga tttttaagcc
     3541 ccacgcccaa cggggaaccg cccgcgaccg attgagagag tttctggatc acgctctgaa
     3601 aataggggtt cggtgtccct ttccggataa tctcgagcga gcggatcagt ggaatgccgg
     3661 cttttaaaag ggtggagagc tggacggcaa aaaatatgag ttcccgggta gtcactcctt
     3721 tcgaaaagag cgtccttccc cccttggacg cgccgtcgct cccgcgcccc ttgagatacc
     3781 cggcaaagag tccggaaaca cccgaggact cctgctgacc tgattgtttg atggagacaa
     3841 ccaaaaaacc ctggtccaga agacgccggg cgcactcgtc ctttgagcgg gcctgaatag
     3901 tcattttttt gagctcggcg gggttatccc gttgacggac ttcacattca aattgctcga
     3961 gcgtttcttc cttcccgacc tggaacgacc gtttattcat aggccacact caaaatttca
     4021 tcgatcgtcg tcagacctct ttcaactttt ttcattccat tctgaaaaag ggtttcgtat
     4081 tttttttctt tctgaaccct gcgtatctca tccagattcg cgtctttcac gattagccgg
     4141 cggaaatcct catcaatcaa aatcacttca taaatcgcgg cgcgccccca gtaaccaatt
     4201 tggcgacatt ttgaacatcc tttggctttg taaatggtgt cagcatggat accgtattct
     4261 gaacgttcct tgtcgctcgg cttataaggc tctttacagt cggggcagag tacccggacc
     4321 aaacgctggg cggccacaag cgcgagactc ccggaaacaa gataaggctg aattccaata
     4381 tcaattaacc gggttaccgc cgtcgcggca tcgttcgtat gcagggtgga aagcaccaga
     4441 tggccggtca gcgcggcgcg aatacaaatt tcagccgttt caagatcccg ggtttccccc
     4501 accagaatca cgtccgggtc ctggcgcaaa aacgccctga gaccggaggc aaatgtgagt
     4561 ccgatgtcag gtttcacctg cacctgattg actcccgtaa tctggtattc cacaggatct
     4621 tcaatcgtca caatattaat gtccgacgag tggattctgt tcaaccccgc gtaaagtgtg
     4681 gtgctttttc cgctccccgt cggaccggtg ataaaaatca acccatacgg ccgaactagc
     4741 gcgttttcaa acaactgctg ttgttcgggc tcaaacccaa gagccttgag attcaggaga
     4801 tccatgcgct tgtccaaaat acgcatcacc actttttccc cggaaacaat cggaatggtc
     4861 gagacacgga cgtccacgcg acgaccttta taggaaatcg agaacgatcc gtcctgggga
     4921 atgcgttttt cggcaatgtc cagtttagaa agaattttaa tgcgggacac aaccgcgctg
     4981 tgaagttcct tcggcggagg cggtaattca tagagggcgc catccacccg atatcgcacg
     5041 ttcatttttt catgaaaagg ctcaatgtgg atatcactcg cccggtcatc cagcgctttc
     5101 tttaacatca cgtcaaccag ccgtaccacc ggagcctgac ccgcttccgc catcgcggat
     5161 tcaagatcca gtttcgtctc ctctttgctg ggctcaagtc cctgggaagc ttcgtccact
     5221 tcggacgagg atacaatctc ctgaaaatcc gtcgcgctgt aataaagctc gatcgcgcgt
     5281 ttaatatcaa gttccgacga aagaaccgtc acgatgtcca gacccgtcat ttttcgaaga
     5341 ttatccagaa agatcagatc cggcattttg ctggccgcga ttttgagcgc ccgttcttct
     5401 cgtgaaaggg gaagaatttt atgctcaagg gcaaactcct tcggaatgat ttccttgagg
     5461 cgctggtcgc cgcggggggt aagctgatcc ggggccaaga atgagatgcc aagttgttcc
     5521 gaaagggtca tccggagatc cccttcgctg atgtacccga ggcggatcaa aacctgcccc
     5581 aaaggctcct tggtttcttt ttggtcgctc agggcgcgtt caagctgatt gggagaaatc
     5641 agtttcttct caatcaaaag ctcgcccagc atactattca gccgcataaa ttttccaatt
     5701 ccttttcagg gtcggtcaat taacgaaccc agccggattt cgtcctcagg tttcgtagga
     5761 caaggctgtc tgatcggatt ttgcgatctt ctcgtccaaa gtatccattg tcgccgccat
     5821 caaccgttta cgaacatcaa aatagctctc aaaatcaatg gggggctctt ccccttcttc
     5881 aagacgcttg taccattctt ttttcttcac ctgataaaaa ggcgcttttt catcgtcata
     5941 gcgaatccgc ttgtccggaa ctttcttgga cataagctcg gaagggtccc gaatgatata
     6001 gggactcagg aaaaggatca gttcgcggtc ttcaattgtc cgggttgtgt ttgtaaaaag
     6061 cgcttttccc acgaccggaa ggttcccaaa aaatggaact ttgcgctcac ctttgctctg
     6121 attgctgaaa agcagtccgc caagcgcgat cgtttggccg tcattgacca tcagcgtggt
     6181 tcgggcggta cggacagtcg tgtctccgga aggctgggcc agagtgatcg atgaattatc
     6241 caccgtcgca aaggtcggtt ccaccgtcat ggtaacctgg tcccgggtgt tgataagcgg
     6301 cgtcaccctg agaatcgtac cgacctcggc gcgctcaaga ccggttgagg tcgttcccgc
     6361 tccttccgcg gtggtcactg tggtcgttcc aatcgcggca ttggtcgcga tcttgatcag
     6421 ggcgggatga ttgtccaaaa ccagaatttt aggtttcgcc aaaatcttca ccttgttcgc
     6481 ctcttcaagg gcgcgaacgc tgatctgaaa actgctaaag tcacgagtgc cgggtgtcgt
     6541 gatgggcccg gaattaatcc ggggatcaaa ctggttggcg gaatccttga acgtatttcc
     6601 gacattgctg aaaaaatcgc cgaaactttt tgtttctatc gtggagccgc cggagcgaaa
     6661 gggccaatcg gtattttgga ccgccccggt caccgtgccc agtagcccgt cagtatcgcc
     6721 ccactgcacg cccagcgccc tgtccagatc ctcataggtc tccaccagta aaacattgat
     6781 caacacctga tccaggggac gatccagttg ggcaatcgcg tctccaatta ttttaagacg
     6841 atcttcagaa tccgtaacca ccaaactatt agttcggtca tccacagaga cagatcctct
     6901 gtcggaaagt attttctcga ccgctttgta aatcgccgag ctttcaagat caatttcttc
     6961 ctgatcctgc gaaagagtga caagcccacc cccgctactg ctccccgttc ccgacccact
     7021 actacttgaa ctgcttgaac tgctcgaact gctcgaatcc gcctcaattt caagcgcctt
     7081 ggttacacgg acataataaa gtttgaaaac ccgggtcata agtggcggcc tctcgggcga
     7141 gtctgccgac gcgcgaaaca aatagatact tctttcgggg agaaaatcat aactgacatt
     7201 tgaaccataa gatatcgcct gaagcgcgtc ttcgaggctg acattatcca gggtcatatt
     7261 aacttcccgc tcagccacct cctttcccgc gacaaagttg atcccgctca gtgaggcaat
     7321 cacgttcagt acattgataa gcggagcggc cttaaagtcg agaaagaatt tacccgcctc
     7381 cgttctctcc acctgcactt taggttctgc gccctgatct tcttccccca gagcgtaaac
     7441 cggcattgtc ccacaaaaca aaacagtcaa ggtaaccgcc acgatgattt tgaatttatt
     7501 ttcagacatg gcttattctc ccctttcttg ggaaactttt agcccataaa acttacggga
     7561 catcataata ttatgaaact catgctacaa ctgattgtct ggctttttcc ctgcgttccc
     7621 ggcgttggcc aagccgtttc agttcatcgt cagacaagat atataaagta ctgcgaactc
     7681 cacccatctc gatttcaacc tcatttttcc gcaccttgag aagcttgatt ccatcacctt
     7741 cctcaccttc ataatagacg tcaccattca agatcgcgta agcgctttct gagccaagac
     7801 cgataccgta cagtcgaact tcaacagtct ctccagcaac ttgatcgact gcctggtctt
     7861 ccactttagg tggcaaaaag ggcgtgaacg gatccctgat gtcgtcttgc aaatctttga
     7921 tttgttcctg gcgctcctgg cgtaacttcg aaacctccaa atcaacctga ccttcctgca
     7981 agtcaacctc gaggtcaacc tgatcctctt gcaaatcagc ctcagcttca tccgcataaa
     8041 gctgaaaggg atttgcattc aaagcaacta aaaaaagtcc aaggatcaca atccttttca
     8101 tgacccgacc gctttctttt gttttaaata agtcacaatg accagtttcc cttgcaatgc
     8161 gttgaccgtg gaatctttag tttcttccat caacagcttc gatcgagtta aactgatttc
     8221 gggaatatcc aataccggat gaatactttg taaatcctgc aacaatttca aaattcccaa
     8281 aaaggaacct ttcaagtcca cttccaggag gagtttttcg tacttgtcag cgggtacaat
     8341 tttgggatta ataccctgaa cttctactcc atttttcccg gaaatgtccg aaatcctggc
     8401 gatcagcatc gaccgttccg aggtttcggc cagaaccgtc tccgactttt caattttttc
     8461 tcccaaccgg acagtttcct gatagagccg tattttttct tccgcggtcc gaatttcctg
     8521 acgtagccag ttgatgtcac gaagagcagg ctgaatcagt acaaaaaaac caagaaaagc
     8581 gcaggcaacg cccaacgcgg caaccatcaa aatttcttta tagcgttcct tgattacaaa
     8641 tcctgtcatc cccaactccc ggtctattcc ccaaccactt tgaactgcgt catcacctga
     8701 tccatttctc tttccctttt aacctcttcc agcttaaact ccgaaaaacg ggatttgaat
     8761 gctccttcgc cctggagttg ctggatccac gcgtcaatag ccgccgtttc ttgttcagcg
     8821 ttaccggtga cgcaccctcc tcgcatggtc attcgattcc gagcgagttt accttttccc
     8881 cccgtggtat tttcaatcga aatataatca atacgaatga cagctggtgt gtttcggcct
     8941 aatgctatta acaattcccc aggcagggat ttcttttttt caaaatctgc caacaatttc
     9001 gcctgggaag ctaacttgac tttccgggaa tctagtgact cgaccgacat ttcaacgacg
     9061 gaagagtcca cgccggaacc cgggcccaga actttctgat actcatgagt taacttcccc
     9121 acataaggct gaaaaacaac caaacgaacc agcagaaaca aaaccatgac tcccaaggcg
     9181 catcccccga ggagggaata aaacgatttc ggtttcattc tccgctcggc gggaggaagc
     9241 aacgcgggct cacccatgag agaactgtat cctgccgagc gaagcgcgag cccataggcg
     9301 ataaaaattg agtccatgat atcagcggag aaaggagtgc cttccgggaa cgtcgctagg
     9361 tcaaaccgca tggcatagtt gaaggcatgt tccagatgtt ccacccagaa ttttaggtcc
     9421 ccgtgccccg ctacaaaaat ctgggtcacg gcctctcccc ccgtcagctt gtaaaaataa
     9481 tctagcgacg ccttcaattc gtcaaaaaaa tgggatttat tctcttcctg gtcctcggaa
     9541 agcagaaaat cccgggacaa ataaacagtc cctttggaca caaacgtcaa gttgacatta
     9601 ccgtccgact ggagagtcac aaacccgcac gttttcactt tctggaacat attgagagag
     9661 gcaaaaacac gacccacagc tgagtgaacc ggttcaatca tgagaacctt ggctgaggct
     9721 ccccgcaaat cgcttagagc ttgctgtaac acttcttttt taatcgcggt cgcggtgatc
     9781 gaataaacgt ttctgtgggt tatctgctca tgataatcaa gaactgactc cgaaagcttg
     9841 aaaggaatat accggctggc ttcaaaacga acggcatcat ccttttcttt tttaggaacg
     9901 gcgggcatca tgaaatggcg agtcacaata tggaacgggc tgactgaagc ggtcacgaac
     9961 gcgccgggct ccttgatcgt ctcaagagct ttgcgaatcg cccgggattc gggggacaac
    10021 ttcctgagct tgtgcgcctc tttgccgacc gcgggcccgt ctcccccagg gttaaccggt
    10081 tcgatcgcgt aggccttgac ctggggtccc ttgaccgtgc taacggccga aaccgcgacc
    10141 acgttatccc gccctatgta aactccaacg ccttttgaca tacgtcgatt attccctgtg
    10201 gataaaaatt atttaaccac gctccacgat cacatgatcg ccttcaatga tttccgaaaa
    10261 acccaacgca tcaaacaatg acacacccgc cactccgggg taaacctgct ctacttttcc
    10321 tcttgtaatg acttttccat ttttgaaaaa ctgaaccccc tgccccgtgc aaactccgtg
    10381 cctggaaccg acatccacta cgataaatgc gtatcggcta tttacggtaa tcactttacc
    10441 tgatattgct tccggagcct tcggatcagg cggcaccacg agagacgcca attccttata
    10501 ttgctccgcg tcccgctggg tcgtcgtcaa ttgagcccga agatcccgca ccacagtttc
    10561 acgttcaacc agggtttgtt gtaaagcatc gatttcgttc tggtagcgta aggcaagctg
    10621 aaaaacttcg tcccgatatt cttttgagtc caaacgagaa ccaccagcat cctcccggtt
    10681 ttcctgtaaa ttccctctca aatccattaa ttgttctttc aaacgccgaa tggtcttttc
    10741 ctgatccaga agaatactct cccaggccgc ttggccttca tctccaagtc ccgcatccga
    10801 cgtcaacgcc gtctcgcgat tcgcgccctg tcccaataaa taaagtgaca cccccgcaac
    10861 caaccaggga atcaggggcc acactcttcg ccagacagac tttcgcttcg gtttgcgctc
    10921 gtggaaatcg gagacttctt tccggagggg ttgagattcc gcctcaacat taaaatcaga
    10981 tttcgaagta ctgggatcga gggggatttt cgattgaaaa agattttctt cggcctggag
    11041 atgcttccgg attttgtgga gatcgcgcct gcgtaaaaat ccggaaccgg tttttaccag
    11101 atttggtaaa accctccaag gagatcgggg agctttcctg tttgagtttg caagagaaga
    11161 cgtctcaatc tgctcaatga ccccttgcgg attatccggt tgtgtttcac tcaagatctg
    11221 atttccttat cttggacaag tcagatgtat ttatcagatc gaatgatact acacctggtt
    11281 ccatttcaga aatcattttt aaaaaatccg agctgtcgct gaccgttcct cacttctttt
    11341 ttttagtttt tttggctacg gcggatttcg actttttctt aacgggtgtt ttcttgacgg
    11401 aggccgcgat tttgggtgtg gccacgggag cctgaatttc tttagtggat accgttccac
    11461 gctgtttgag atgcgacggg acggaaacac cggacgcgat atccttggga cgatgacccg
    11521 cctttcggat cgcccagcac tcggggcagt aaaccggtct gtcgccgctc ggtttaaaag
    11581 gaacttcaca tgttttgaga caatccgcac aaaccgcctg ataaagtgat cggggatttt
    11641 gaggatgaaa actcttgccg ggatggaccg agagctggga agggtgactg accgaggaat
    11701 gctgaaatcc ctggatgacc tgtctgattt ccgaagggag attcgacacc cggccggaaa
    11761 cctgatccat cttttgctcc agggccccaa gcctttcgac gagcttcatc atcgcggtca
    11821 cgagatccgg cgtgacggaa tgactggttt tggagcgtgc ggctttcttt atcttcttgg
    11881 cttttgtcat agagttttcg ctctccttta cccgggttca gttaaccttc agaaaagtcg
    11941 ccaacaattc cccataagtc cgcgtcacgg gaaattgagg aaattccttc gcgattctgg
    12001 aaggcggacg gaaaaacgcg ccgaaatccg ccgccttgag catcgtcacg tcattgtaag
    12061 aatcccctgc cgcgatcacc ttgaaaccca aagacttaag tccgcgaacc gcccgttcct
    12121 taccgtttct ctgccgcaaa acatacccgg atataaatcc atccgaatcc gtcttaagcc
    12181 agttacaaaa aagagtgggc tgatccaatt ttctcatcat cggcccggca aattcataaa
    12241 aagtatcgga caaaataatg acctggtaac gctccttgag cctttttaaa aaacctttcg
    12301 ccccggggat cgggggcatc gcgcggatca ctttttgaat atcacgaagc cggatttttt
    12361 cttttttcag aatcccgatc cggtacttca tgagcttgtc ataatcgggt tcatcccggg
    12421 tcgtaatctc cagctccttc acgcccgtcc tcttggccac gtgaatccac acctcgggca
    12481 tcaacacccc ttccaggtcc agacagcaaa tcatgacgtc tcctccgcgt tcaatttaaa
    12541 acacatccac ttcttcaaac acttcatcaa aattttcgcg cgtgaaaatc cgcgccgcat
    12601 gcgcggatta tatccaagaa aatggggcgg ggctactttt cgttaattct atctgttact
    12661 tccaggcgat cagcgcttca ctttgcctca cagggaggca tgagataacg ctggacacga
    12721 aacttccagc cgccaatacg atctgtttcc ttggagcccg gaggagaaat ctcaacccgg
    12781 cgatcaagaa ccgccttggc cgctctcaca aattccggaa gaatttcgtc ctcttctttc
    12841 gtctccccca tcgcccgcaa aacctgaagc aatccccagc cttccccttg agaacgctcc
    12901 gtttcgagaa ctccttctcc cttaaaattg atgtagtcga taacggcaag aggcccgtaa
    12961 ggcgcaccaa gaacgcggcc gagtttcgcc tcaatcaaag gtctctcctc atcggaaacc
    13021 gcggacagga tatccggcag tgttttacga acccgctcga caataaattg cgtctggaga
    13081 tcccgggtcg ccagtaaaaa atcccggagg cttttcattc gctcactctg gctatcccgc
    13141 aaaaaagaat cccgatcatc ccacggaagg tcctcgcatt tttccagcca ctcgggaaga
    13201 gtctgcccgt gccttttcat gaaaaggagc aattcaggaa atgtttcccg aaaagggccg
    13261 cgccggcccc tgggatacca gatcgcgtgt ccgatcccaa gcgaggcaaa atcttcatcg
    13321 gcgggccaga taaccagttt ctccggattt gaactgcact cgttaagata aatgatcttc
    13381 tgaagacaag ccatttcgtc gtcatcaagt tcaaccttga aagccgccca caaaggttcc
    13441 gtcatcaggc acaaacaaag cattccgcac gcaattttat ggtttaggaa cattcggttc
    13501 atttttttct tcgcaggacg cgcgcgtacg aaaaaaccat taacgagata atcgcgaccc
    13561 aactcaccgt tataaaaatc cagcctgaaa gcttcatggc ttcctcccga gtttggaacg
    13621 tcccgtggat tttatcaaag gtttcccgcc cgattgaacc ctggacgcct tttgccggat
    13681 tgattttatt ttctccaaaa gatccaggac ggcgccagtt tcacgcagac ggtacctagc
    13741 catcgtccgt tggctttcgc caacataaat ggccacatcc gaccggcgca aaacagcaaa
    13801 agcatcctca tccgttttgt catctccgac gcaaaccgtc gtcagacctt tccgttgcgc
    13861 cggcgtgagc gatcggagaa taagttgaag cgcccgtccc ttgtcggaac aaccccgcct
    13921 cggccgtatt tcccaaactt ttttcccggg ccgaatttta aaaaaaatct tcgccgaaaa
    13981 gttgctccag acgtcacgaa aaatccgccg ggccttcgct accgccgaag gtgaaagatt
    14041 ccggtaatgg acggtcgcgc tgtaagtctt gttttcgatc aatactgcgg ggacattttt
    14101 catcgcccgc ttcagcgcgt ccgtgagttt taaaatgaaa ccgcggagct ggatggcatg
    14161 agaatcaatc tcgggatgac ctccaagatt ccgttccaaa ccatgatttc ccacgtaccc
    14221 taaccctttt agaccaacct gtttttccag gtcccccagg gcccgaccgc tcatgataac
    14281 gattttaacg ccgggcattt tcgagagttt tttcaagacg attcgcgcgc gcgggtgtaa
    14341 ccgggcgaga aatggccggc tcacgatcgg cgtcaaggtc ccatcataat ccagacaaat
    14401 aagaagattg ggcgatgagg ctaccacgtg gattgtttta tcgtccagcg ctctcaacgt
    14461 catgagatcc ccctcctaga aagaccttac aagctcggtg atcatgtcag ccgcccaccg
    14521 gtaaatgttg tgttccttga cgatgctccg catcttccgc atccgctctt ttctttcttc
    14581 gggtaacatg gtgagcgcct gatggatggc ctccgccgtt ccctcgatac cgtaaggatt
    14641 caccagtagc gcgcttgaaa gctcataact ggctccggca aaacggctta aaatcaagac
    14701 gccgtcctcg tcctcacgcg cggagacaaa ctctttggcc acgagattca ttccgtcatg
    14761 aagggaactc accagacaaa aatcggcaag ccgatagaag tcgaagattc tcttctgccc
    14821 gtgatgtcct tcgagaaaga ggatggggcg ataatggcct ttttgaaaac gctgattgat
    14881 cttttcgatc aatttttcga cttccttcaa atgagactgg taacttccaa tcaacgttcg
    14941 ggagggggcg cccaattcga caaaaacaag ttctccttgc agatagggat atttttcaag
    15001 aacccgttcg atagcccaaa atcgttccag aagtcccttg gtataatcaa tccggtcaac
    15061 gccaatcccg attttctttc ccttcaggcc ataatcctga cgcaattgct cgaggggatc
    15121 ccgatccgtt tcctggaccg ccacattagg ggctcccgag acactaaccg ggaaggaacg
    15181 cacggtgctt ttttttcctc cccgcgtaac ttcaaagttc gtccagttaa ttttgctttc
    15241 cagagcgcgg tccaccgtat ccagaaaatt attgcaatgg aactgggtat gaaatccaat
    15301 catgtcgctc ccaagcatcc cctcaagaat ttcctcctgc catggacaaa tgccgaacgc
    15361 ttccgcgtta ggccagggaa tatgccagaa aagcgccacc ttcgcgtcag gtctccgctc
    15421 tttgatcatt cgcggaagaa gggcgaaatg gtaatcctgg acgagaacga tgggaggttc
    15481 gtctccaaac tcctccaaaa tcgcgttcgc gaacttccgg tttacttcct tgtaataatt
    15541 ccagtcttcc atccggaaaa cgggccgcac gtgactgata tgacaaagcg gccaaatacc
    15601 ttcgttggaa aaaccgtagt aatacccttg ttcttcttcc ggcgataacc agacgcgctt
    15661 gagcgtaaat gacggtttcc acggcgggac tctcacctta ttttccgcat cgacggattc
    15721 ctggtcggcg ttaccggccc catgggcaat ccacatacct cccgtcgcct gcatcacggg
    15781 atccagcgcc gtcaccacac cactcgcggg agtaatgcac tcgatcccgt tcccgttcct
    15841 tcggtgaatg taaggctccc ggttggatac tacgtaaaac tgcttatcac gaaattttgt
    15901 ttttaaataa tccttgagcc tctccttcgt ccagatggag ccccgctcat tcgccagtcg
    15961 cgactgttcc accagatcca gtcgcgccgc cttcaggctc gccgccatgt ggaccacccc
    16021 ataaagcagt ttttcgattt ccccttgttg ctgaattccc cgggatgccg cgctctccat
    16081 cccaaggttt ttgtgaatca tggaggcaat gcgcgaaatt gggccggtta cgttccagcg
    16141 gatcaccagg agggagacaa tcgagaggat tcccgccaga atagaaaagg tcaacgcgga
    16201 atttttccag aattgccgga tctgaagcac aataaaagtc cggtcatgga tcagtcccaa
    16261 agctcccacg acctgcccgt ccctgatcag aggaaccgcg tggtaattaa ccttccggcc
    16321 gtcccaatgg ctgttgagat cgagaggaag attttcatcc atgaccttca tcgactcttt
    16381 tttgaaccag tcttcacggg aaagatgggg cggaaaagcg atcctctgtc cgtctttcat
    16441 gtccaccaca agaccgatca acctcgtgtg accctggaat ttttcaataa atttctggat
    16501 ccgcgaaggc tggtcggtac gctcaagaag cgtttcgatc gcctctttaa atgttttggc
    16561 cagtacggtc gcgcgaagtc tcagttcttc attgagccgt ttttcttcac ttttcgcctc
    16621 aaaataagaa aagcccgcga ccacgatgac cgtggcgatc aaaagcgaaa gaatcaggcg
    16681 aaacgtaatt ttcatgacat gatcccgtcg atcctccggt taggcgtccc gtttgtccgg
    16741 attctgtcct gtcccccagt aaagaagccg gtgcggaaaa gcgatctgga tcttttcctg
    16801 atcaaatctc gccttgagac ggcggcgaaa ctcccgggcc acctcccatt gtttgatcgg
    16861 aagcgtcttc atgcgaaact tgagcgtaat ctgtgaatcc gagaaatcgt ccaccccgag
    16921 catttccaga tcattgagaa ttaaatgttt gaaataaccg tcctcccgca tttcagcgcc
    16981 aagctctttg atcactcgaa tcgcgtgatc aacatccgta tgatacgcaa cgcccacatt
    17041 gagaagcgcc tgggagtatt cctgggtaaa attaatcacg gtcttgattt ctccgttcgg
    17101 gatataaatc acgcgtccct ccagatctcg taatctcgta tgacggagat tcacggcctc
    17161 cacgactccc cccagtccct caatctgaac gacatcattg atccggaact gcccttcaac
    17221 gagaataaaa attccgttaa taatatcccg gatcagatac tgtccgccaa acccaaaagc
    17281 cagtccgacc acgccggcgc cggccaaaag aggcgcaaca ttgattcccc aactgctgag
    17341 tagcatataa ataacgcaaa gccagatcag agtcgcgcca atccatttga gcaagtgcga
    17401 aaaagtcttc atcctgagaa catctccact ctcgcgaatc gcgtccgttt tggaaatcgc
    17461 tttttcaaat ttttcaaggg caagccggag caggagtacg agaacccacc cgaccacaag
    17521 tatcagaaca gccttcaggg tttgcgtcac ccagggggtt tccatgattt tttccatcgt
    17581 aagggattta tccatgattc aatccttaaa agtttgaagg tttcagtgat gatctaaaac
    17641 atttttatgg tatcttcccc aaaagcagaa tgcaagaaga accgggcaag cggcaaaaaa
    17701 acattcccga acctgatctg ccgttcccgt caaaaatcgg aacgctcaaa ttcactgttt
    17761 atattcttct tttcccaggc tccgcgccgc gatttccccg aggctggatc gttccggttt
    17821 aagagcgagg taagtaagaa taattccagc gctggacaag aacactatca cgccaaggat
    17881 tttcgataga ctaattccgg catccagaca tattcccatg aggaagggag ctgtcgccgt
    17941 cgaaaaaaca atagcggttg aaaccatgcc gcggatggaa ccgaggtgac gggttccgta
    18001 aagctcggca tagagggcat tgccgacggt cattccgtaa cccatggtaa ttccggagcc
    18061 cgcgaaataa aaaaagatcc agaacggatt tttcgcgagc cagaggaaca aaatgcccaa
    18121 cgtaagcgga atgaggttca acgcgaaaat ctttctcgcc gaaaaacgat ccgtcagggg
    18181 acccgaaaaa aaagaaagga tcgctcggca aatcgcgtac acgacaaaac cggccggcaa
    18241 gagaagagga tcccagtttt tccagcggat cacggcggcg tgatgaaaaa aaagcgcggt
    18301 caaaaccgac ggcgtcacaa gaatgggaag cagtaaaagg tgaaaccgcc ggtctttcat
    18361 cacgcgggaa acgggccagg aactgtcttt cggatcgggg gcgttcagat cttcccttat
    18421 atcgggagcc gcgcgaccgg gctggggttc gttccggatc agataccgcg acatcggaag
    18481 aaaaatcagg aggatcatcg ccgcaagcgc ccaccatccc tcccgccatc caaacacggt
    18541 aatcaccgtt gaaatcagtc cgggaagcag ggattcgctc aagggatatc cgaggttagc
    18601 gatcccgagg gcctggcccc ggtgttcttt tttcaggcgc gccatcatgg tttgcgacgc
    18661 catcgcgagc cctccctgtc cgaaattgcg cacgagaaaa aaaccagcca gtagcatcca
    18721 gggagcctgg gccgtcgcga gaaaaaggca tcccgcaagc aacaaaaccc ctgccacggt
    18781 aacaaagcgg atcggacgtt catgatccag acgaaacccc agaaacggaa gaaaaaaagc
    18841 ggaactcaaa gtccccacga aaaaaagacc ggagatcgcg gtttggctta accccagttc
    18901 ttcacacatc gggggaataa aaaaagaaat caggtagact tgtcccgggg cgctgaaaaa
    18961 ggtcgcgagg attccaaaaa taaggagctt ggtttttttt cggatgagcg cacgcacctg
    19021 tcaacctttg acaaaatgat tcacgggacc ggcgccacgt ccgagccccg gcgccgtacg
    19081 aatggctttc gtgacgaatc ttttcgcgat tcggatcgcc gcgagaagct cccgcccttt
    19141 ggcaagctcg gccgtaatgg ccgcggaata ggtgcaccct gtcccgtgcg tatgccgtgt
    19201 ccggattcgg ggagccttca gaaggaccat ccgtttcccg tcgcaaaaga catctattga
    19261 agccccttta aggtgtcccc ctttgagcaa gacggcccgt gggccgagct caagaattcg
    19321 ccgcgaagcc ttcttcatgt cagaaagatt cccgatacga atccccgcca gaatctccgc
    19381 ctcgggaatg ttgggcgtca caagggccgc gtgcgggata agacttttct tcaacgcttc
    19441 gactgatgag ctggcaagca ggcgggcgcc gtgcttgctg accatgacag gatcgatcac
    19501 aagcgggaag cggaatgttt cggccttgcg cgcgatggca cggatcaatc cctcattccc
    19561 gagagctccc gttttggcgg cgcgagggca aatatcttca agaaccgtct caagctgtga
    19621 gatcacctgc gccggcttga gtggatgaac cgcgcgaacc ccgcgcgtat tttgcacggt
    19681 cagaagagac acgaccgcca ttccatagac gcgtcgctgg tgaaaagttt tgagatctgc
    19741 ctgtaacccg gcgccgcccg acgggtcgga accggcgatc gtaagagctg ttgggatcat
    19801 cgcgaaaatt cgtttccctt cattctcaag gtcatgcttc cccggatttt aggagggaga
    19861 gactatttca gtgtcttttt gaaaatttcg ataaactgct cgggagaaac tcccgcgtaa
    19921 agctggatcg ttttatcctg attcggcgga tgaacaaaaa aggaggggta gcccataact
    19981 ctgtaccggg ccgcgatcgc gtactcccgc cgtcctgact cgggattgat tttcacctta
    20041 ataacatccc gcatgaactg gcgcacccgg ggatccgaaa gaattttttt ctcaaaaacg
    20101 cgacaataag ggcaccattt gacattgatg taaaccgcca tgggccgacg cattcgttca
    20161 taatcggcaa acgcccgctc atagccctcc gcgtccgaat accagcccgt cgtccaatcc
    20221 cccgtcagtt cctcgctcgc cgcgctcgac gactcctcca cgggagtgac cgtttcatcg
    20281 acctcagccg cgcacgatac cgaggacacg ctcagccagg acaaaagaaa gcacatcaaa
    20341 aatttccgga atcgcatcaa ggcacctctt tgaagggtta gtccgtaaaa gtaacgtcgc
    20401 gtattgtatc agaacctcta gaaactgaaa gccggtgaat cttgccccag taaagaatcc
    20461 atcactgagg cgacatctct cagaaaaaga atgatcgcga tcgcgatcaa aatgaccagt
    20521 atcgtccaag ctatcttcat gaaccttgaa aacttggaac tctgccacag caagggcacc
    20581 gcgaacgggc caaccaccaa aagcatgaaa acgacatagg ggacagaatc accggtgaat
    20641 tttctcggtt ttcccatagc cacctgatag gcgtcaaccg ccgagggaac catgacaaaa
    20701 agacagatcg gagcgagaaa tatcaggttc agcaaggatc ggcttgtcac aagagcgtac
    20761 gcgagacccg cgctcacgca aagtagcgtc aaacctttcg tccggtctcc gagataaatc
    20821 tgccccattc ccgggacgaa aaaagaaaga accaaagcta tccacttgtt tttcatgttc
    20881 atgttatcta aggccaaacg tgaaaccggt tttgactttt tcgattaact caggaaggac
    20941 ttcccgataa tcacggacca ggccaaaatc ggcatggcga aatatagccg cctggggatc
    21001 ctggttgatc gcgatgatct tgccggattc cttcatcccc gccaggtgct ggatggctcc
    21061 ggaaatggcg acggccagat agactttggg gcgaactgtt tttccggtct gtccgatctg
    21121 ccgggcataa ggggcgtaac cattgtcaac cgcgtgccgg ctcgctcccc agtccgcgtt
    21181 gatcccctgt tccctgagcg cttccacaag atcttgaatc agccggaact cgtcacaatc
    21241 cccggctggt cttccacccg agatgacaat atcggcgaca aacaagttgt cgccgccccc
    21301 ggattcacgc cgggtctcaa gcacttcgac tttaaaatca gtatctttaa aaaccggctt
    21361 gaattcggtc acagtcccct tgcggctggg atcacgcggc aaaatcttga aagtcccggc
    21421 gcgcgccgtc gccatctgag ggcaccaaaa accgatgatc gtcgccagtt tgctttcacc
    21481 gtaagtgggc cgtatcgact gaagtacggg ataaaagacg gctttcttga tccggtgaat
    21541 atagtccccc acttcaagac tcgtgcagtc ggcagtcaca ccggcccgca cacggcttgc
    21601 caaccggggc gcgagttccc ggcctgacgt cgtggctcca aaaagcgcga tctcggggcg
    21661 atcatgttcg ataatttgcg agagcgcggt cgttgtcgga aggatagtgt attctttcaa
    21721 tcgcggatca tccacgatca ccacctcatc ggcgccatgt tcaataattt cccgcgcggc
    21781 tcctttaata tcatgcccaa tcatcacgga acgcaccttc gtaccaagct ggtcggcaag
    21841 cttgcgggcc tggctcaaaa tctcgagcga tgaacgcgcc ggcgtttgtc cgtgcatttc
    21901 aacccatgtc atcacgccgc gcgcgccgtt cctgaaatca acctttggaa aaccggaatc
    21961 cggttcgaca gcaaccgctg tgttcgcgcc actggccggg gctgaccggg aagactcatc
    22021 cgcgacccgg tttttcaaat cttcaatcgc gctcacgagg ccatccgtta attcccggcc
    22081 ggtatgtccc gatatcatgt tcactggcgg acgatctttc tgaatgtcaa ccaccttgga
    22141 tacaagggtc ggggaacccg tcaaacctac cacggttgaa tcggcgcccg tctggtcaag
    22201 cgaccagact tcaatctttg tgtcgcgggc ccgaacagcg cctgctaggg aaggatgccg
    22261 cgccggagta gcggtcggag cgatggagat gagacacggg ataggcagtt tcagcacctg
    22321 ataaccacct tcgataattc gccggacttt cacgtggtcg tcgagaaact ccactttttc
    22381 gacgaaagta gcctggggga tgtcgagatt ttccgcgacc tgggacggga catgcgcggt
    22441 atccccgtcg gtcgtctggc gtccggaaaa aataatgaac gggtctttgc aaccccgttc
    22501 aaaaccaagt tttttaatca acgaagaaat agcgtatccg gtcgcgaacg tgtccgaacc
    22561 gcccagtttt ctgtccgaaa gaagcatggc gcggtcatat cccatggcaa gcgctttctt
    22621 cagggactgc tcgaagctcg gcggccccat ggaaaccacg atcaactccg cgtccggcgc
    22681 gtgttttttt agctggagcc cctgctccag gccgaacgcg caatccgcgt taatcatgga
    22741 cttcgctttt gtccggtcca taagcccgtc ggcccccatc cgcatttcgg tcgggagcgg
    22801 cacttgtttc attaaactga tgattttcag acccactttt tctccgtcac ttgtctcgcg
    22861 tcacaggccg cgcgaaaaac ccacccgcgt ggctccgcga agcgtatcta aaggttgtcg
    22921 tattccggcc cgtcaccgcc atacggcacc gcccattcaa cgttgccgaa cggatcttta
    22981 atatcacagg ttttgcaatg cacgcaattg gcggggtgaa ggtgtatgtc tttctttccg
    23041 gttttcggat ccgtcacaac ttcataaaca tcggccgggc aaaagtactg gcacggagcg
    23101 ccatacttcg ggatgcacac gtcacggcaa aggttgagat cgtgaacatg gcagtggcaa
    23161 ggctgttctt catcgtgctt cgaacccgaa taaaaaacgt ccgtcacttt gtcataggta
    23221 agttttttgt cataagacaa atctttttcg tattttttat tgaaagggcg tttcacgtcc
    23281 gaaagcgggg tgtaacaagc cgcatcctca tgaattttca ttttaccgga cggcgtaatt
    23341 ccccgtcccc cggtcactat ctgggtcccg aaatggaacg cgccaagaat cattccgttc
    23401 gcgaatcccg cgcgaaaatt ccggacgcgg tacaaatctt tgtagcccgg actctcccgg
    23461 aataaatcct cgtaacgttt taactgttcg ctagagaagt catttttcaa aaacgcttca
    23521 aacgcggcct tcgccgaaag catacccgat tcaatggcca gatgaatacc tttcaggccc
    23581 ggcatactga gaaatccagc ggagtcacca acgatcatca ccgaatcgtg gaaaagcttc
    23641 gggagagaat aaaatccgcc ttcagggatc gtcttggcgc cgtattttag aattcgtccc
    23701 ttttcgagaa ttttcgctat cctggggtgt ttcttgtaaa gctgaagcgc cgcgtgcgga
    23761 tcaaaggtcg ggtccttgta atccagcgcg gccgcgatgc ccaaggccac cgtccgtttt
    23821 gtcattccat aaataaatcc gccgccaaag tgattgaatg aaaaaggata gcccagcgta
    23881 tggatcacac gtcccggttc aaacgctccc tccggcactt cccaaagttc cttcaccccg
    23941 gtcgaataga cctgcgggtt gaggccctgg agcttatgtt tttgaataaa ttttttggca
    24001 agactgccgc gcgaaccttc cgcgagaatc acaagcctgg ccaggacctt cgtaccggcc
    24061 tgataattat ccatgggctt gccttcttta ctcactcccg tatcaatcat tttcgcgcca
    24121 accacgcgcc cattttcata aaggagctct tccaccgaaa accccgggta aacctgaacc
    24181 ccttttttct ccgcgatctc tcccagataa cgcgcgatct ttccggcaga agtgacgaaa
    24241 tttcccttgt tggacatgaa cggcggagcg aaaggcattt tgatcgcggt ctttgacgtg
    24301 agacggtaaa attcatcccg ggtcacagga gtttcgaacg gaaggtcctt gatctcaacg
    24361 ccgggaagca atgctttcaa ggtcgaggga ttgaccacgg cgccggagag agaatgactg
    24421 ccgagagaag cgcctttttc gacaaggaga atgttgatcg gcagtttccg ggtggccgat
    24481 tcattgtgac gggccaccaa atccgcgaga tgaatcgaga atgccagacc ggcaacaccg
    24541 ccgccgacaa tcaaaacatc tgttttaaca ctgttatttt caatcatgac ggacaaaatt
    24601 cccgctggtt ggaagactgg ctaaaggagg cggcattcta gcataaacct aattttttca
    24661 atgaaaaaat caccgccgga cacaaagcgc aagtgtctta tttccgtttt gccagaaagt
    24721 gcttgatcag gtgggcctgc ttgattcctt cggattctat caaaaaagaa tcgtgcccat
    24781 agccagcatc gatctcacaa taggtcgcgt caatattatg catcttaagc tgacggacca
    24841 tatcatgcga ctggaccgac ggataaagcc agtcggattt aaacgcgatc acgagaaaac
    24901 gcgcgtgatc aaggctcccc ttctcaaaga gcttgtctcc cgaaagatca aaataatcca
    24961 tcgccttggt gatatagagg taggagttcg cgtcaaaacg tttcacgaaa ctttgccctc
    25021 ggtattgcag gtatccttcc acttcaaaat ccggtgaaaa ctggaatcct ccgttgtctt
    25081 ccttcactct gcgcgcgaac tttttctcca tcgattcatt actcatgtaa gtaatgtgcc
    25141 caagcatgcg ggccaccgcg agcccgtgtt cgggcaagtt tttaccgtaa tagttccctt
    25201 tttgccagtc cgggtctgcc atgatggcct ggcggccgac ctcattaaaa gcaatctgct
    25261 gtggggaatg tttcagggtt gccgcgatcg gaatgacgca ttcaatccgg tccggataaa
    25321 gcgcggccca ctgaagggcc agcattcctc ccatggaccc gccaatcacc gcgtaaacct
    25381 tccgtatacc caactggtcg atcaacataa cctgggcatg gaccatgtcc gcgagggtaa
    25441 ccgccggaaa ttccagcgcg taaggttttc cggttttggg attcgtcgaa gaagggcccg
    25501 tgcttccctt gcaccccgcg atgacattag cgcatatcac gaaatatttc tctgtgttca
    25561 gcgcgcggcc cggcccgatc atgaaatccc accagccggg ggctttttcg cctttgtgcg
    25621 accccgccgc gtgcgcgtcc ccggtaaaag cgtgcatcac gagaatcgta ttcgacttct
    25681 cagagttcaa ctcgccgtaa gtttcatacg ctagggtcaa gggagcgaga gtctttcccg
    25741 actcaagctt gagcccttcc ggaaatacat gaaatttcgt ttcaaccgtt ccaagacctt
    25801 tagtcatcgc gcgcaatcct caaaaagcca tgtggacaaa tatcgttctc ccgtatcaca
    25861 aatgacagtg acgatggtct ttccctttga ttcaggccgc cgcgcaatct caagagcggc
    25921 ccaaacattc ccgcctgtcg aaataccgcc caaaatccct tcctgcttcg ccaactgcct
    25981 cgccgtttct ccggcatcct catttttcac tttgataatt tcgtcgtaga tccgggtgtt
    26041 gagaacgcgc gggatgaaac ccgccccgat cccctggatc ttgtgaagac cgggttttcc
    26101 tcccgacaac accgcggaat cggccggctc aacggcgata attctagcag aaggctttct
    26161 ctttttcagc tcttctccca cacccgtcaa tgtccccccg gtcccgacac ccgcgacgaa
    26221 aaaatcgacc ttcccttccg tgtcgcgcca aatctcatcc gcggttgttt tccggtgcgc
    26281 ctcggcattc gcgggattat taaactgttg aggcataaaa ctgccgggcg tgttcgactg
    26341 gatttcggag gccttctcca cggcgcctcg cattcccttc gatccttccg tcaaaaccac
    26401 ttccgctccc aaaatcttga gaagctggcg gcgctccacg ctcatcgtgt ccggcatcgt
    26461 cagaatcagc ttgtagccgc gcgcggccgc gacaaaagcc aacgcgatcc cggtattacc
    26521 gcttgtcggc tccacaatgg ttccgccggg cttgatctgg ccattttttt ccgccgcgtc
    26581 aatcatcgcg actcctattc tgtctttaat gcttgacatc ggattgcggg tttcaagttt
    26641 gaccagcacg tcagcccgca ggcctgccgt taaccggttt aaccgaacaa gcggcgtatc
    26701 gccaaccgat tgagtaacgt cttctagtat tcgtgccata gggcacctcc tggtttacga
    26761 cggtaatgat attcaaacaa gaaaaccagg cttctgttgg atccggtttc cctgttctcg
    26821 taaatcttca agctttttgt aaggcctggt caatatccgc caggatatcg tcaatatgtt
    26881 caaggccgat cgacagacga ataaaatcgg gcgtcacacc ggtcgcggcc cgctcttcgt
    26941 cggaaagttg ctgatgcgtc gtgctcgcgg gatgaatcgc gagagtcttc gcgtcgccga
    27001 tattcgcgag gtgtgaaatc agtttaaggg aatcgataaa tttcttgccc gccttgaggc
    27061 cccccttgat tccgaacccg atgatggcgc ccgccccgtt cggaagatat ttttttgagc
    27121 gcgccttttc agaactggaa gcaaggcccg gataatggac ccacgccact ttgggattct
    27181 tctcaaggtg ccgcgcgacg cagagggcgt tttcggaatg tttcggcatc cgcagatgca
    27241 gggtctcaag tccttgcaaa aacagaaacg cgttaaaggg agaaagagcg caccccaggt
    27301 cccggagcac ggtcagcctg gccttgacga tataagcgat gttcccaaac ggtttcaagt
    27361 gatcgacaaa attgatcccg tgataactcg ggtccgggtc cgcgatcaag ggaaatttcc
    27421 cgttcgtcca gtcaaatttc ccagaatcga caatcagtcc tccgagcgac gtcccgtgtc
    27481 ctcctataaa tttcgtcgct gaataaacgg tcacgtcgac accgtgatca aacggcttga
    27541 gcaggtacgg cgagaccgta ttgtccagca caagaggaat cccgttttta cgcgcgactc
    27601 cggccaaccc ctccagatcg gccacgtcaa gtttgggatt accgatcgat tcggcataga
    27661 tcgctttggt ccggggggtg atcgctttct gaaatccctg caaatcgttc gacttcacga
    27721 acttcaccct gatgccaaac ctcgcgaaag tgtagtgcag aagattgtag gttccaccat
    27781 aaaggttatc cgccgagacg atttcgtcgc ctgcctgcgc gatattgagt agcgcgagcg
    27841 cgatcgcgga ctggccgctt gagacggcaa gcgcgcccac tccgccatcc agctgggcga
    27901 ttctcttttc caaaacatcg gtcgtgggat tcatcagcct cgtataaata tttccggttt
    27961 ccttcagtcc gaacagatcc gcggcgtggt cggtactctt gaactggtac gacgtggtct
    28021 ggtaaatcgg caccgcccgg gctccggtca ccggatcaac ggtctgtccg ccatgcaata
    28081 cgatagtttc acttttaaat ttcttttctc tctggctcat aacgttccct ccgctaacgg
    28141 tcgtttaagg taaaaaattc ccttcatcaa ataaaataca tattcgcgca ttccgaacga
    28201 atcttttttt cactcgcctg ttcaaggctc gctccgtcca gaacacccac catcgcacta
    28261 taaagttccc gcatcacgcc gcgaatggcg caagaatgag tcccctggca ttcggggcat
    28321 tgcgccacgc gtccgggctc aacacagacg actggcgcca tggatccttc aaaaacacgg
    28381 atgacatcgc caaaagtaat ctcgccgggc tggcgggcaa gcagataccc gccccccctt
    28441 cccttgcgac tcaataaaag tcctttgttt ttaagctcaa ggaggattac ctctaaaaac
    28501 tttttgggaa tcgactccct ttccgcgatt tcagccgcca gcacgggttc cccggagctg
    28561 aatctcgcgg ccaggtgaag catggcgagc agggcatatt tagtcttatt cgatatcata
    28621 gtctactaat cctatcggaa tgataggaat atatttacag attaaaaccg tgtcaagcat
    28681 ttcattaatc tcatgattac gacaaatcgc aattggaggc cgcaagtcgg ctccgcgctc
    28741 acttcgccaa caaacagcca taatagattt caccctacta ataatagagg acaaaatccg
    28801 cgtcctgtca ttcaccttcc caatttcctg gatgtcgttt accctcaaac taacagccat
    28861 caaaaggaga ataatcatgg gaagcgaagc gtcgtgggaa tatgtagtac gggattttct
    28921 ggaggttaaa tgcgcgctga aagaacagga gcaagagtat cttttggatt ttttgaaaac
    28981 aaaaggcatt cgtgaggaac cggttaggat ttatcccgcc ttctccatcg ccgactgcct
    29041 tgtggtcgtc acggacggcg cgaaggcgaa acggagatgg ttttatcacc ggggaaaaaa
    29101 gaaaatagaa gaataacttt cacgacggcc ggcgaacaaa cccaaaagtc aaaatggttt
    29161 tattcaccgc gcatcatgcc ccacgttacc agcgttagat gataacccga ccgcttcacg
    29221 aagctcccgg gattcaaagg aagttaaaaa acggccttcg ccaagccgaa gattggaaag
    29281 tttgacgggc ccgaaggaaa tccgcacaag cgtcacgacc tcataatcaa atctttgaaa
    29341 cacgcggcga atctggcgct tttttccttc cctgagttga accatcaaac ggcgtttcga
    29401 aaccggacga atctgttcga gcttcaaatg accgtcaaac gcgtgaaaac cgttaatgac
    29461 ctttcccgaa tcttccggcc gccacgccct gtccaaggtg acttcatacc atttttcgac
    29521 atgataccgg ggatgaagaa gcctgtgaga caattcaccg tcattggtaa gaagcaaaat
    29581 ccccatcgaa tccttatcga gccttcccac cgggaaaaca cgaacctttc cccggcttcc
    29641 ctggcgggtc gcgttgacaa gttgcctcgg aaggagatca taaaccgttt ttttagcgta
    29701 ggcgtcctgc gccgtcacca cataacctac cggtttattc aggatgtagt acacgttcgg
    29761 agagcattca aggattttgc cccggtaagc gatcgtgtcc ttcgaaaaat ccacctggcg
    29821 tcccaaatca cggacgattt caccgttcac ggtcaccgcg ccattggtaa caaacacctc
    29881 gcattttctc cgtgacccca aacccgccgt cgccaacgcg tgagcgatcc tcatgaccct
    29941 ttcgttcctt tggcgagttc cgcgtaatac agggcgcgcc cctcagcggc ccatttgatt
    30001 tcataattgg ttttcatcgt ccccccaaaa atccgctcgt tgacagtttc gcggacattg
    30061 tcccagagtt cacgggtcgc cgctatccct tttttcattt ccgtaaaata gtcgagatca
    30121 tcggtcgcga cttcgaacaa ccctcccggc gacaaccggg agtggatcaa cttcaaaaaa
    30181 tcttccgaaa acgtgcgccg ggcccggtgg cgtcttttgg gccaggggtc cgggaaatac
    30241 atgtgaaaaa cactgacgct caggggcctc agagcctctt caagaaattt tctcgattcg
    30301 gcctgaatga actggatgtt cctggcttcc tctctttcca cccgcatttt ccgccgtttc
    30361 atgtacttgg cgatccggtc aatcccgata aaattgatcc ccggattttc ttccgcccgc
    30421 gcgacaagaa agctcccctt cccgcatcct atttcaacct ccaccggatt gtcgtttcca
    30481 aaaagctccc gaagggaaat ggggggataa gtccctttta caaacgccca agacatacca
    30541 gtgtctccaa atgacgggtt cggggaaaaa aatcaaacgg gataatccgc tctattttcc
    30601 agcgatcttt aagcgaggcc agatccctcg acagagattc cggagaacag gataaataaa
    30661 aaagggccga aatcccgtcc gattgattaa gtgtctggat ggcgctgggg taaagcccgc
    30721 ttcggggcgg atcaatgagc gccacacagt gccgtcctct cagggcgctc aaaatttccg
    30781 gcaaaagttt ttcaacctgt ccctcaaaga ccttcacatg ctccagcttg tggtaggcga
    30841 tattatgttt ggcgagccgg accgaatcgt atccggattc gatcatcacc acctctttcg
    30901 ctagatcggc aagcgccagt ccgaagagcc ccactcccga ataaagatcg agaaacaccg
    30961 tttccggctt ccagacgctc cccgccgata tcgtgtccgc gacctttccc aagatcgaaa
    31021 gattcgcctg aaaaaaagtg tccagcgaat aaaaaattct tctcccgcgg atcgtcaccg
    31081 aaaaataatt ttccggatcc atccgaagtg accgtcggcc aattcctccc cacgccacct
    31141 tgccttcttc tccgacgcga accgtgagat tggcgttacg gtactcaccc gccacggctt
    31201 ttacggtcgc ttcttcccga agcttcggta aaaaatcgtt gatctctttt cgggcgatcg
    31261 ggcacacggt cacgtcttca agcttgtggg tgttttcgag cataaacccc atccgccaac
    31321 ggccttccct gtcccttcga aacgtaaggt ccaaacggtt tctgtaataa tatagttccg
    31381 gcgaaggcac caacggttca aaaaccagtg gatccaaccc ctcgatagta ccggtcaaaa
    31441 gatcccgaag agccttttct ttgatggaaa gctcctcttc gtaggaaatg tcctgatact
    31501 tgcatccgcc gcaagaaccg aacaatgagc aaagaggcgt caccttcaag agagtttttc
    31561 ctttcccggt taaaatatcc caccactcaa aaacgattgc gccgttcgtt taattcgggg
    31621 gatcatggct ggcgttttgt tttttctttt gaaaaaagga aatcgagaga aatttaggaa
    31681 acctctcccg ttctctcttg ggcgaccgcc agcataattt tcatcaataa atcgcggttg
    31741 aaataatccc gattgaaaag ttacgggaag aggcgggaag cgccccgcta gaggcaactc
    31801 caggtttgac ggcttttttc aaaggcacgg atcgacggtt catgttcgag agtaataccg
    31861 atatcatcca gcccccgaag aaatcgctct ttaatcgcgg gatcaatttc aaaatgaaac
    31921 ttgagatcct gggggcgggc gtgaagcgtc acggtttggt cctgcaggtg aatggacgcc
    31981 tcaaggcccg cttcatcggc gatggcctta aaaatctcat ccacttcccg ggatgaaagt
    32041 tcgatcgtca aaagaccgtt cttcacggag ttattcctga aaatatccgc gaaggcggga
    32101 acccggtgat ctccgtcgtc tacccacggg gcgatcacga cacgaatccc gtactgacac
    32161 accgcccaga ccgcgtgctc acggcttgac ccgcagccaa aattgtttct cgcgacaagc
    32221 acggtcgcgt tcctgtaggg ggcctgattc aggacaaatt ttggatccgg ctttccgtca
    32281 ggcagataac gccagtcata aaaaagcgac tcgccaaatc cggtgcgccg tattgatttc
    32341 agaaactgtt tggggataat cgcgtccgtg tcaacgtttg cgcgatcaag cggcgccact
    32401 ttccctctaa aattttcaaa cgctttcatt ttccattctc ctgccgtgtt ccatcacgcg
    32461 ctcatcgcgc gcgttgataa acctttaaga accgcgaaag ggcgttcatg ttttccggat
    32521 atcgacaaaa tgtccggcca ccgcggccgc cgcggccatt tccgggccca ccagatgggt
    32581 gcgtccgtct tttccctgcc gcccttcaaa gttacggttt gaagttgagg cgcaccgctc
    32641 ccccggtcgc aactgatcgg gattcatcgc gagacacata ctgcatcctg attcccgcca
    32701 ttcacatccc gcttctttaa aaatcacgtc cagaccttcc gactccgcct gggccttcac
    32761 tttctgcgac cccgggacaa caagaacccg gacgccctgg gccacttttc ggcctttcaa
    32821 ataattcgcc gcggcacgaa gatcttcaat ccggccgttc gtgcaactgc cgatgaaaac
    32881 cgtgtcgatc cgaatgtccg tcattttcat cccggactta agtcccatgt aatggatcgc
    32941 cttttccacg tccgcgcggc tgtacccctg cacctgttcc ggagccggga caacaccatc
    33001 cacgtccgtc accatgccgg gacttgttcc ccatgttacc tggggagcga tccgtgaagc
    33061 atcgatcacg aactcccggt caaacttcgc gcctggatcc gtcgggagtg ttttccagca
    33121 agcgatcgcc gcctcaagat cttcgccttt gggggcgtac ggccgactgg tttcagtaaa
    33181 ataacgaaac gtggtctcat ccggagcgat catcccgctc cgcgctcccg cctcaatcgc
    33241 catattacag accgtcatgc gcccttccat cgaaagcgcg cgaatcgcct ctccacaaaa
    33301 ttcaatcgcg taacctgtgc ctcccgcggt cccgatcatg ccgatcaatt tcagaatcat
    33361 gtctttggcc gttacggaag acggaacccg gccagtaaac tccaccttga acgtcttcgg
    33421 ttttttctga aggagacact gggtggcgag aacgtgttct acttcggacg tcccgatccc
    33481 gaacgccaaa gccccgaacg cgccatgggt cgccgtgtga gaatctccgc aaacaatagt
    33541 ggtccccggc aaggtaattc ccagctcagg cccgataata tggatcacgc cctgccggtc
    33601 actgtgaaga tcatacaaag tgatactgaa ctccttgcag ttacgggtaa tcgcctccat
    33661 ctgctccttc gcgatcaaat cccggatatt aaaacggtca ttcgtcggca cattatggtc
    33721 catggtcgcg aacgtcagtt cgggatgacg gaccttgcgt cccgccagac ggagcccttc
    33781 gaaggcctgc gcgctcgtca cttcatggac aagatgacgg tcaataaaaa gaaccgccgt
    33841 cccgtcggga agcgtggtca ccacatgttt ttcccagatt ttttcaaaca atgtttttgg
    33901 cataggggca caattatatg gctttactcc aaaatcgcaa ggattctgca ccgtcccgtg
    33961 tccggcgtcc ggttgcggcc ctatctgggc aatcaatgag acaatctcct gcaggcctca
    34021 agaaattaac tgcctaaagt gggatgcgcc cgaggacagg aaacgattga ctcccgaaaa
    34081 atgaaaacga tacaattttg ggacgtagcc agaattaaag tcaaggttta gtcttaaatc
    34141 ttgaaactgt gcccaaaaag atctgacgac atacagcctg aaatttcaac agctcaaact
    34201 gaaagcaaat cgaagaatag ataattatgc aagatatttc actggaactg aaacggactc
    34261 aaatcgaaat tttaaaggta atcgaccgta tctgcagaga tcatgatatt aaatattcac
    34321 ttttctatgg gtcgttactg ggtgccgttc gccatcaagg catcatcccg tgggatgatg
    34381 atcttgatat ctgcatgtct cgtgaggact acaaccgatt cacccgtatt tggaacaacg
    34441 tgaaccctca gggatacatt ttacagaata aagaaaatag ccgcgctttt gctgaggctt
    34501 ttacaaaaat ccgcaaggac cacacctgtt ttttacagaa aggcgagcgc gccaacgcat
    34561 accataatgg tatttttgtg gatattttcc cgattgacag attgcctaac gggaaaatga
    34621 agagattcgc atttaaatta aactgtaagt tgtatcagct gtttacgcgc gaattggttc
    34681 cttcgaaaat taatactata gcaggcgcca ttttttctat tctgctgttg tgcacgcccc
    34741 atttttgtag aatgggctta agagagaaat tactcaagaa aatcaccaaa tacaattcga
    34801 atactgcttt ggaaaccgtg aatatcacga caaaggcatt cctggaaaca cctttttcca
    34861 gcacaatgct taataactat accgaactag atttttctaa tgagaaattc atgtgctatg
    34921 aagaatggga tgactgtctg accagaatat acggggacta tatgcagctt ccacctgaag
    34981 aggagcgcgt ttggaagcat caccctatca ttattgattt tagccacaac tacgatgaat
    35041 tggagaatgc aaaataaaca aacctatccg cgcatcgcga tcatgggctt tatgagccgc
    35101 ggtggagcga agacagggga aaggttaaca aaacaggaga tttgggagac caatatctcc
    35161 ctgcggatgg cgaacagtac ccaaattaac ttataagaca aggggg
//
LOCUS       DAOSIO010000002        23676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_2381_length_23676_cov_4.885695, whole genome shotgun sequence.
ACCESSION   DAOSIO010000002 DAOSIO010000000
VERSION     DAOSIO010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 23676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23676
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2381_length_23676_cov_4.885695"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PK590_00185"
     CDS             complement(<1..313)
                     /locus_tag="PK590_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23048.1"
                     /translation="MPRGIRLERKKESILLNGLKRPFLVKKVFVPMSYKTKAAAVPCV
                     KTGDQVEKGAKIAALDRCEGVSVFSGLAGRVEGVVEKKMPDGSRQSCIPIASEDDFKH
                     PP"
     gene            complement(332..1072)
                     /gene="bioD"
                     /locus_tag="PK590_00190"
     CDS             complement(332..1072)
                     /gene="bioD"
                     /locus_tag="PK590_00190"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HOG23049.1"
                     /translation="MKGGIFITGTDTEVGKTIVSAGLALCLRDRGIKVGIMKPIATGC
                     YGQEDRLVSQDAAFLMEAAEGQFPPLHSPSRFRNPLSPNVAAMLEKKEINVDHILKSY
                     RELESHYDYMIVEGIGGLMVPIKKDYYVANLIRDMKLPLIVVSSAGLGAINHTLLTLD
                     AATVRGLEIKGIIFNRVSVANYSLAELTNPKVVHDLSGVPILGSLPEIDDLQTESCKF
                     GKLKEVFRERIQVDKILGEAFPRSLHKA"
     gene            complement(1168..2550)
                     /gene="bioA"
                     /locus_tag="PK590_00195"
     CDS             complement(1168..2550)
                     /gene="bioA"
                     /locus_tag="PK590_00195"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002173346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HOG23050.1"
                     /translation="MNTRQLMAKDRQWIWHPFTQMQEWQEEDFPVIERGEGCCLIDTE
                     GRRYLDGVSSLWCNVFGHRVPEIDSAIKKQLGKIAHTTFLGLTHPSAIELAEKLIALA
                     PKPKFGVAKERQGRLKRVFFSDSGSEAVEIALKMAYQYWQQKGQPRKKKFAKLTNAYH
                     GDTIGSVSVGGIDLFHQIYHPLLFETIAIPSPYRYRWPSGEDPEKVKTESLAVMEKVL
                     RTYHEDLAAFVMEPLMQAAAGMIDQPKGYISRVRELTRKYNTLLIFDEVATGFGRTGK
                     MFAAEHENVTPDLMTIAKGMTGGYLPLAATLTTEEIYRAFLGRYEEFKTFFHGHTYTA
                     NPLACAAAIATLDLFKKNKTLAKLQPKISLMKNRLKSFYQLKAVGDIRQVGIMVGIEL
                     VKDRGTRELFDPREKIGVRVIECARKKGAILRPLGSVIVLMPPLAMTIKEINKLLDIT
                     YQAIKEVTEE"
     gene            complement(2689..4656)
                     /locus_tag="PK590_00200"
     CDS             complement(2689..4656)
                     /locus_tag="PK590_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG23051.1"
                     /translation="MSLLAIDRISKSYKIGEVEVRALQEVSLTIQAGEFLAIMGPSGS
                     GKSTLMHLLGFLDRPDSGTIRLMGKDTSRLQEEEYAFLRNRVIGFVFQQFNLMARASA
                     IENVSLPLLYSEDRANDLARAREMLEKVGLSHRMKHRSNELSGGQQQRVAIARALVNK
                     PLMILADEPTGNLDSKSGKEILEIFKELHGQGMTVILVTHDEEVGKLAERVIRMQDGK
                     IVEDKTRSDKKAGPMLQKDIFKQGGLSKKKKYSYAECSEHFRQAKRVIVSHKLRSFLS
                     MLGVLIGVACVVTMLALGRGAGEAIKEDLARMGSNLLTVRPGSVKVRGASLEAGAATR
                     LKLEDAQAIQKVYSVKRVGAQVMGSGQLVFRDKNWSTRVMGTAPEYAAMRNQEPVAGR
                     FFTHEENLSRSRVVVIGPTIQKVLFGDEEPIGKTIKINRVSFQVIGILPSLGADAFRD
                     RDDLVVIPLITAMRRLMGKDYLDQIDVEITETEQMPAAQDEINELIIRRHRLTPDRYD
                     SFNIRNFADIQEALKSTTKTFAILLGSVAAISLIVGGIGIMNIMLVSVKERTREIGLR
                     KALGATSRDILIQFLVESVLITFLGGLAGIALAGLISWQIAMLAQWKMIITPGSLFLA
                     FIFSAGVGIVFGLWPAKQAASLNPITALRYE"
     gene            complement(4653..5657)
                     /locus_tag="PK590_00205"
     CDS             complement(4653..5657)
                     /locus_tag="PK590_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG23052.1"
                     /translation="MKFKISNLPRSAKVASVITGILLGAKIFSSCGAPGSDLQVVRIE
                     KTTRGNLSLTVTATGTVKPYNRVEIKAPIAGRIDEVLVREGDEVTQGQVLARMSSIER
                     AALLDAARSRGGEAVRRWEEAYRPAPLLAPLDGTIIVRSVEPGQTVTTADPVLVLSDR
                     LIVESLVDETDLALISLGQSAEIILDAYRSEKITGRVDHIAFESRLENNVNIYAIDVV
                     PEEEPPSFRSGMSASVTFIVKEIKDVLLVPSEALTARPKEFPNPSGASVAVYRKGFGG
                     KPEPVAVKIGETDGRMTEIKEGLKEGQEIAIVRKRQEKNGNPLSPSSSSRSGGSQKRS
                     "
     gene            complement(5638..6957)
                     /locus_tag="PK590_00210"
     CDS             complement(5638..6957)
                     /locus_tag="PK590_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG23053.1"
                     /translation="MFEKHVSVLGVAFLLGGCLTVHLPKAEIERQSEPKAVTWVALVR
                     QALANNPDLREARARVESKARSRDIAFGDYLPAVDGGVHKSASRTTSGPTSDRLNLDI
                     SARQSFFDGLGTTGKWLGARKDLEAERFAYNEMSADICHRLRTSYINVIRLEKLFETN
                     RLISERRKQNAEMVRLRYEAGRENLGSALRAEAIAEQAAFDVRQTERQLEKEALRLAR
                     ESGGEFVLPLRVDDDLEKMTKFVKLPEEDYAGLADETPAVLRLLKVAESAKAEMMSAQ
                     SSVWPQVDGTYEYGYSGDRASHLKDQSSIGVSVTMPFFEGGKNVAAIRKAKADYDAAE
                     NAVRSARDETVAQLAAARAPFIDAVEVVQVRKKFLEAARERAEIIRTEYATGLVNFLD
                     FDTAEQENANAEKEYVESLANVFIQQSQWAKIRGVTLEEAYDEIQNQ"
     gene            complement(7150..7806)
                     /locus_tag="PK590_00215"
     CDS             complement(7150..7806)
                     /locus_tag="PK590_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006892645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG23054.1"
                     /translation="MKRLFVIILMLPLFTTIFQDPGFTAEVTNMRLLPPDTEGGKPLM
                     QALSERKSSREFRPDELPPQVLSNLLWAANGVNRPDGRRTSPSAKNTQEIEIYVAKKE
                     ALYLYDAKEHALTVVIPEDIRSRIGRQVFVQDAPVGLIFVSNTGKFRGGSEEDALFYS
                     ATDTGYVSQNVYLFCASEGLSTVVLGTIDRDQLAKTMKLQPRQRIILTQPVGYPKNRS
                     "
     gene            complement(7813..8967)
                     /gene="bioF"
                     /locus_tag="PK590_00220"
     CDS             complement(7813..8967)
                     /gene="bioF"
                     /locus_tag="PK590_00220"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HOG23055.1"
                     /translation="MKQANRDELSDLKKNNLYRQIRVLKNSDGIHAEWRGKKITLFCG
                     NDYLGLSRHPRVIRAAEQALKKYGVGAGAARLVSGSTTAHEALEKKIARLKNKERALV
                     FGSGYLANLGILTAFAREGDTIIMDKLCHASLVDGARLSQAELRVFPHKKYEKCEAIL
                     HKSVGRRKILLTESVFSMDGDLADLKELVRLKKKYKALLVVDDAHGMGVIGKHGHGAT
                     EKFSKEIDIIMGTLSKAIGGLGGFVAADDPYVDYLVNFSRPFIFTTALPPVLSASALE
                     SLSVIEKETALRKKLWDNIRSIHVGLSEAGFVLGKPESAILPVMIGNEKRALEAFQSI
                     LKEGVFIPAIRYPTVAKGKARLRITVSASHTEADIRALLKAFKSYGKKEA"
     gene            complement(8984..10189)
                     /locus_tag="PK590_00225"
     CDS             complement(8984..10189)
                     /locus_tag="PK590_00225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOG23056.1"
                     /translation="MKRRVVVTGIGFLSPIGNTVEDVTQNLRNGISGKEDELVSDVFG
                     YQFPPVFRVKNFNPRHYGTHILDPFIQYAVAAAESAVQDAKFDVASVDPYVVGLSISS
                     SKGGVHTMDRFRDRFLKNPSAIMGARIYTSSVPNFGDQWIARRMKIKGPAKCYIAACA
                     TGVAATIAGFRMVQEGQAEYCLAGAADSSIVPLMLAGYNNMRALAKQEIRPFDQRRDG
                     FLVGEGAAVLFLETLESARARGIRVYGEIVESACAQDSRQAILFDRDDHALSRAVKNL
                     MERSAIRPHEIDYINLHGTGTTHGDLYETAEMKVALGEEAYGIPMSSTKAATGHMLGA
                     TGAFEIAATLLAMKESFIPPTLNLEEKDPACDLDYSPLKAKEKKMTVSLKVSMGFGGH
                     AMAVLLRKI"
     assembly_gap    10293..10392
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10507..11283)
                     /locus_tag="PK590_00230"
     CDS             complement(10507..11283)
                     /locus_tag="PK590_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG23057.1"
                     /translation="MNQYLSGKKILITGGTSELGTAFVKQAIAHGAEIHFTFFSAAER
                     AKELETIGAKKHKADLSSTEAIDSFLEAFCRENKTLDILIHNAAVAADHLLEKMTEEE
                     WDRVLATNLKAPFYLTQKLLPLLEKRGSRSARTKNSHFSLSKIFMIVSRAGTEGLAGA
                     SNYAAAKGGLIAMTLALARELGPKKILVNAVNPGFMRSRLTAALSEKVIQRALGRSPL
                     ETLADPVEAANFLIYLSSDLVSRVTGQVFHFESRPLQQNG"
     gene            complement(11287..12321)
                     /gene="bioB"
                     /locus_tag="PK590_00235"
     CDS             complement(11287..12321)
                     /gene="bioB"
                     /locus_tag="PK590_00235"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HOG23058.1"
                     /translation="MFRDPRASSAKTLIQKLKEKVLSGGEISYQEALSLAAIRAVDDL
                     DFLRSSAHELTLHFKDSRPDLCALINAKSYLCGEDCKFCSQSAKYDTGVQRYPMMSPE
                     KIVEAAKRCEENGARNFCVVTSGETLSAGEFEQVRKTFQLLSEETGLGIDGSLGNLTE
                     EQISELKKVGLRRFNHNLQCSREFYPEIVSTHTYDERMETLNFLKKNDVEICSGGIFG
                     MGESPEDRLKLAFELKRYAPHCFPVNILNPRPGTPLAGQPPIDPAEAIKMIAIYRFIH
                     PHANIKLAGGKELNLGIFFLEKALKGGANGLVVGGYLTTCGNPIAEDFALLKRAGYIV
                     ERESAVAQTA"
     gene            complement(12338..13555)
                     /locus_tag="PK590_00240"
     CDS             complement(12338..13555)
                     /locus_tag="PK590_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOG23059.1"
                     /translation="MMIKQMLKDRALTDKERKNLSILEVIRKNGPISRTDISKLTELN
                     IVTVSNYVNHYIKKGLVVEGDLDESTGGRKPVLVELNPKAGYIVGVGLNMMSMVGVLV
                     DLEINVVTELKRERLPENSEKVIQQMIDLAAEIIEKAEIDKTKIVGVGVGIPGIIDER
                     GRTIRWPQSLGDKDISVCTSIKDTFEKRLGIPAFVENDANAAVLGEKWLGLDRDVRNM
                     VYMFSGVGCGILINEEIYRGATGAAGELGITSIHASKEYTQEIATQLGRWEMDIGMAR
                     HAKEQIEKGEPSVIKDYVHGDLEKIHFRDIVRAIKEKDALAMKVAEQAGQELGKKVAF
                     IVNLLNPEVVVIGGGIEDCGAPLLDSIKSSVKEWSVEEAVTPVKIIPSAFGENAVALG
                     VVGIVAREVFAQA"
     gene            complement(13699..15828)
                     /locus_tag="PK590_00245"
     CDS             complement(13699..15828)
                     /locus_tag="PK590_00245"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOG23060.1"
                     /translation="MTETQTQSQRKRFLELDKTLKSAFFMGCFFLLIPAFLFAAVRNE
                     QREDSSVSANNARGRIQCGADETLEITKIYNTVPPNKSLLTSPQFLVIDDFANYKEWK
                     NRLHNHWRLENGKARSVTMEHSREDGREMRPGQSLKILFELDPRQHFTLSTSLGKLDM
                     SKARFLAMKCKLKPNPKHGFNGRIRVSLTDWTGRSVTRDITETCAKSEKWQEAILPVE
                     YFKGLDFDQLDRLSITVLGRHKRSKANLWLDDIVFFGHNEVGFESVLDNIKDFPKVVK
                     YLSGKQRLLSMTKDSDFLNAIGQDTWRYFEEAANKENHLVLDHIKVGDFPMVGVYTSP
                     TNIAMDLICTVAARELGYISKESAQERAHLVLDALQKLPRWRGFFYNFYETNKLSINR
                     KFISSVDNAWLAAALIVTRQAFSGAEAKRASKILEEMNFEEFYDPDTNHIAIGYDLER
                     AGLTPYHYGMMISEARIISFIGIGKGDIPKEHWWYLFRTAPDAWDWQTQKPSGQWVTK
                     EGVEYFQGYYTVDSKEVVPSWGGSLFEFLMPTLIIPEDKLSPESFGRNNRVATEIHRD
                     YALNEKEYPVWGISPAAVSNGRRWFYGEYGIKKLGTKGYPEKAIIAPYAALLALETLP
                     GDVVKNLRKMLELYDIYGEYGFYDSINLKNDLVTNQYLSLDQGMSLIAIANYLKKGII
                     QNFFMKDPIAQKAQSVFEGERFFKDRK"
     gene            complement(15825..18278)
                     /locus_tag="PK590_00250"
     CDS             complement(15825..18278)
                     /locus_tag="PK590_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017814978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 36"
                     /protein_id="HOG23061.1"
                     /translation="MSQKKSKKKRVALSQKTTKEQQKFKSSYGYFTADGREYVITRPD
                     TPRPWVNVISNGDYGTIETQTGSGFSWRGNSNLSRITRWDQDLIRDNWGKYIFLRDKD
                     NGKYWSGTWKPCMTKYDFFEVRHGQGYTVTTTKKNGIKFEKTLFVDMTEPVEVWKIVL
                     TNETEKKRRLSVFSYFDWCLGNAADTHREFHKTFIEVWIDKKNGCLYGEKRAPLVPGY
                     ISTGMTEKPLRGFHGAFPKPVAYDGDKEAFLGRYGEQHAPAAVEAGKCRNSEGKWGDA
                     AASLQVDVTLGPKQSKTIVFTLGSTPTREEAVRIIKKYQTPGMADRELNKVKAFWDTL
                     IHSTEVETPDESLNFMTNTWLKYQAISGRLWAKCAYYQSSGGYGFRDQLQDCQIFFST
                     RPELAKKQILLHAEQQFPDGTVYHWWHPGTNIGAITHSSDDLLWLDFITLNYLEETDD
                     HAILDIDVKFLPDPKDPEKKVTSAPLYEHLLRAIDKALSRFSSRGLPLIGECDWNDGL
                     SHVGNKWKGETIWLGHFLYGILSRIAPLMKQRGDTAKAKDYLRRAELLKEAINQYAWD
                     GEWYWRATKDNGEILGSKNCERGKIFLNAQTWAVICGTATPERAKTAMASAKKWLYKK
                     YGPLLLTPGYNKTDPTIGYITRYAPSVRENGGLYTHAGTWAIQAECLMRNAEQAYELY
                     KSFNPVLRGLKPEIYFSEPYVTPGNVDGPDSPNYGRGGWTWYTGSGAWYAVVVLNWLL
                     GIRPTSKGLRVDPVIPKAWPGFKMKRLFRGTTYHISVTNNGQGQGVKELKVDGKKQPG
                     DLIPDFQDGQNHRVEVVIG"
     gene            complement(18323..19612)
                     /locus_tag="PK590_00255"
     CDS             complement(18323..19612)
                     /locus_tag="PK590_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOG23062.1"
                     /translation="MNKRWVKSLFVYSILLTSFFLVGCGAELSGDGKIPVKVSFWGTP
                     EEIDIITQALSDWHAEHPTIKIVFEHTPYTGYTSKILTRIAGGAAPDIIATEVDYFVT
                     FASKGVLEDLTPYMEEDTAGFKKEDFFPQIIDRFTYDGKLLALPRDIAPFACVYYNKK
                     LFDAAGIPYPTDDWNWNDLLRLSRELTKKDESGRVTQYGFYGWAWQNFVYGNGGALVD
                     DVKNPTRTLLDDPKTMEGLQFYADMSNLYGAMPTPVAFMNFGMGADRMFANDRIAMFL
                     SGIWETPLFRNYNISWDVVMFPKNAEGKRAFGTGGTGYCILKSSKNKKEAWEVIKALT
                     GPKGQELFAKRGLAQPARKEVAGGLAFAKDPQPPANKKMLNDAVQHVVFSPFHPQWRE
                     IEEKILKPKLETVFNGKKRAEQVVKEVTAEINQLLNS"
     gene            complement(19719..20663)
                     /locus_tag="PK590_00260"
     CDS             complement(19719..20663)
                     /locus_tag="PK590_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOG23063.1"
                     /translation="MKKALGRGLNALIPDSYVQGKEMEKNIPASAHVTSSAPAAPSLA
                     VSPAPQGAFRLIPVEQITPNENQPRQHFDPVGIDELANSIREKGILQPVIVKMTGQNQ
                     YQLICGERRFRAAKVCGLTQVPAVLKDVASEDFLEWALIENIQRQDLNPLEEAEAYQK
                     IMESRALSQEDVAKRVGKNRTTVTNTIRLLRLPEEVKTYIVTGQLSAGHARALLGLLT
                     PEHQRQMAKRIVEENLSVRQVELMVNRSNAHKRKAKTARHLSPEIVDLENRLVHHLGA
                     QVKIYPRKNQKEGRIEIHYSSLDDLDRILQKINLAPNG"
     gene            complement(20921..21655)
                     /locus_tag="PK590_00265"
                     /pseudo
     CDS             complement(20921..21655)
                     /locus_tag="PK590_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(22169..>23676)
                     /gene="mnmG"
                     /locus_tag="PK590_00270"
     CDS             complement(22169..>23676)
                     /gene="mnmG"
                     /locus_tag="PK590_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOG23064.1"
                     /translation="SYMLRALMAEKNLTLKQGEVTEILVRDGTCYGVRTKAGETFSGK
                     HVILTTGTFLQGLIHIGEEHYPGGRCEEPPAIGLSDSLMRHGIELVRFKTGTPPRLHR
                     ASIRWDLTEEQKGDEPPPFFSHRTTHREVEQMSCWLTHTNSEVHEILRKNLTRSPLYA
                     GRIVGIGPRYCPSIEDKIVKFADKERHQIYLEPEGRDTDEIYVNGFSTSLPKDVQDTA
                     LHKVRGLENAEILRFGYAIEYDAAPPTQQKHSLENKKIKNLFLAGQINCTSGYEEAAG
                     QGLIAGINVVKKLRGEKEFTLARTQAYIGVMIDDLVTCGTNEPYRMFTSRAEFRLLLR
                     QDNADMRLMRLGYEIGLVKNEEYQKFLERCERIKDDVKKLRIKRVGQDTAYQQIKKPE
                     MSYKLLIEKGLHASKLTENEKHFVETEIKYEGYISRQISDVKKFTKIEKRKIPQTICY
                     DDLSNISREAREKLKKIKPESLGQAARIPGISSCDISLLAIYIEKAAKKNS"
BASE COUNT         5558 a         6289 c         5302 g         6427 t
ORIGIN      
        1 ggggggggtg tttaaaatca tcctccgaag caatcgggat acaggactgc ctggatccgt
       61 cgggcatttt tttttcaacc actccttcta ccctgcccgc gagcccggaa aaaacagaca
      121 ccccttcaca ccggtctaac gcggcgatct tcgcgccttt ttcaacttga tccccggttt
      181 ttacgcaggg aacggccgcc gctttggttt tgtacgacat ggggacaaaa acttttttga
      241 caaggaaagg acgcttgagg ccgttaagga ggatggattc ttttttccgt tcaagacgga
      301 tacctcgagg caaagttctg ctcctttcag tttaagcttt gtgaagagat ctcggaaagg
      361 cctcgcctaa aattttatcc acctggatgc gctcccggaa aacttcctta agtttcccga
      421 atttacaact ttcagtctgc agatcgtcaa tttcgggaag gcttccaaga atgggcacac
      481 cggaaaggtc gtgtaccact ttcgggttgg tcaattcagc aagggagtaa ttagccaccg
      541 aaaccctgtt aaaaataatt cctttgatct caagcccgcg aaccgtcgcc gcgtccagcg
      601 tcagaagcgt gtggttaata gctcccaatc cggcggagct gacaacgatc aggggaagtt
      661 tcatgtcccg gataaggttg gccacgtaat aatccttctt gatgggaacc atcaatcctc
      721 caatcccttc cacgatcata taatcataat ggctttcaag ctcacggtag cttttgagaa
      781 tatggtcaac gtttatctct tttttttcaa gcatcgcggc aacattcggg gaaagagggt
      841 tgcgaaaccg ggacgggcta tggagcggag gaaactgccc ttcagcggcc tccatcaaaa
      901 aagcggcgtc ctgagatacc agacggtctt cctgaccgta acagccggtg gcaataggtt
      961 tcatgattcc aactttgatc cctcggtccc gtaagcaaag ggccagaccc gccgagacga
     1021 tcgttttccc aacttccgta tcggttcctg taatgaatat tccgcccttc accactcaac
     1081 tccagagcaa aaaaccacaa gaccccgcgg cagaaaaaac ccttttcgtg cggcgcataa
     1141 tatgtggtgt gtgttttgca ttatgaatta ttcctcggtc acttccttaa ttgcttggta
     1201 tgttatatca agcagtttgt tgatctcttt tatcgtcatc gccaaaggag gcatgagaac
     1261 aatcaccgac ccgaggggtc gcaaaatggc tccttttttt cgcgcgcatt caatcacgcg
     1321 gacaccaatc ttttcccgcg ggtcaaagag ttcacgcgtc cccctgtctt tgaccagttc
     1381 aatcccgacc atgattccaa cttgccgaat atcgccaacg gcttttaatt gataaaaact
     1441 tttcagccgg tttttcatca aactaatttt cggttgaagc ttcgcgagag tcttgttttt
     1501 tttgaaaagg tccaaggtcg cgatcgccgc cgcgcacgca agcggatttg ccgtgtaggt
     1561 atgcccgtga aaaaaagtct taaactcctc atagcgtccg agaaaagccc gataaatttc
     1621 ctcggtcgtc aacgtcgcgg caagcggcaa atacccgcca gtcatcccct tcgcgatggt
     1681 catcaaatca ggcgttacat tttcgtgctc cgccgcgaac atttttccgg ttcgcccaaa
     1741 tcccgtggcc acttcgtcaa aaatcaggag cgtattatac tttcgtgtca attctctcac
     1801 gcgagaaata taccccttgg gctggtcgat catccccgcc gccgcctgca tcagcggctc
     1861 catgacaaaa gcggccaaat cttcgtggta tgtccgtaaa accttttcca tcaccgccaa
     1921 agattcggtt tttacttttt caggatcctc tcccgagggc cacctgtacc ggtaaggcga
     1981 cgggatagcg atcgtttcaa aaagaagcgg gtggtaaatt tgatgaaaca ggtcaatccc
     2041 gccaacgctc acggaaccga tcgtgtcccc gtgataagcg ttggtgagtt tggcaaattt
     2101 tttctttcgg ggctgtcctt tctgctgcca atactggtat gccattttga gagcgatctc
     2161 gaccgcttca gatccggaat ccgaaaagaa aacgcgcttc aaacgccctt gtcgctcttt
     2221 cgctacaccg aatttaggtt tcggagccag ggcgatcagt ttctcagcta gttcgatcgc
     2281 tgaaggatgg gtaagcccaa gaaaagtcgt gtgcgcgatt ttccccagct gttttttgat
     2341 agcgctatcg atttcgggga cgcgatgccc aaacacgtta caccaaagcg acgaaacccc
     2401 gtcgaggtac cggcgacctt cggtatcaat caggcaacac ccctctcccc tctcaataac
     2461 cggaaaatct tcctcttgcc attcctgcat ctgcgtaaac ggatgccaga tccactgcct
     2521 gtctttagcc atcaattgtc gcgtgttcat agaccgttat tttaacctga atttctcctg
     2581 tcgagtatga gaaatatcgt ttgagaccaa caacaagtat cgcctcatcg ctttccagaa
     2641 ataaaaatga aataaaagaa gcaacagaaa taggagcgat ttcaaaagtt attcataacg
     2701 aagagctgtg atcggattca ggctggcggc ctgcttggcg ggccataggc caaacacgat
     2761 tcccactccc gccgaaaaaa taaacgcgag aaaaagcgac ccgggcgtga taatcatctt
     2821 ccattgggcc agcatggcga tctgccagga aatgagtccg gccagcgcga ttccggccaa
     2881 ccctccgaga aacgtgatga gaaccgattc cacgagaaac tgaataagaa tatcccggga
     2941 cgtcgcgcca agcgcttttc ttaacccgat ttctcgagtc ctttctttca ccgacaccag
     3001 catgatgttc ataattccaa tcccccccac gatcaacgag atcgcggcaa cacttcccag
     3061 caaaatggca aaagttttgg tggtactctt gagcgcttcc tggatatcgg caaaattccg
     3121 gatgttaaag ctgtcataac ggtccggcgt caggcgatgg cgccgaatga tcagttcgtt
     3181 gatctcatcc tgcgctgcgg gcatctgctc cgtctcggta atttcaacgt caatctggtc
     3241 cagataatct tttcccatca gccgacgcat ggccgtaata aggggaatca ccactaggtc
     3301 atcccggtca cgaaacgcgt cagccccgag cgatggcaaa atcccgatga cctggaaact
     3361 cacccggtta atcttgatgg ttttcccgat cggctcttcg tccccaaaaa gaactttctg
     3421 gatcgtgggt ccgatcacga cgacccggga acgcgacaag ttttcctcat gcgtaaaaaa
     3481 ccgccccgcg acaggttcct gatttctcat ggccgcgtat tccggggcgg ttcccatcac
     3541 gcgggtactc cagtttttat cccgaaaaac cagctgtcca gaccccatca cctgcgcgcc
     3601 cactcgcttg actgaataga ctttttgaat ggcctgcgcg tcttccagtt ttagacgagt
     3661 cgcggctccc gcctccaggc tggctccgcg aactttaacc gacccgggac ggaccgtcaa
     3721 aaggttcgac cccattcggg ccaggtcttc cttgatggcc tcacccgctc cccgccccaa
     3781 ggccagcatc gtgacaacac aagcgacccc aatcaagact cccagcatgg aaagaaaaga
     3841 tctcagctta tggctgacga tgacgcgttt cgcctgccgg aaatgttccg aacattccgc
     3901 ataactgtat tttttctttt ttgaaagccc gccctgttta aaaatatcct tctgtaacat
     3961 cggcccggct tttttgtcgc tccgggtttt atcttccacg attttcccgt cttgcatccg
     4021 gatcacgcgt tccgccagtt ttccaacctc ttcatcatgg gtgaccaaaa taacggtcat
     4081 cccctgcccg tgaagctcct taaaaatctc gagtatttcc tttcccgatt tggaatcaag
     4141 attgccggtc ggctcatccg cgagaatcat taacggttta ttcaccagcg cgcgggcgat
     4201 agcaacccgt tgctgctgtc ccccggaaag ctcgttagaa cgatgtttca tccggtgcga
     4261 caatcccact ttctcgagca tttcccgggc ccgcgcaaga tcgtttgcgc ggtcttccga
     4321 gtaaagaagc gggaggctga cgttttcaat cgcgcttgcc cgcgccatca gattaaattg
     4381 ttgaaacaca aatccaatga cgcgattacg caagaaggcg tattcctctt cctggagcct
     4441 agaagtgtct ttccccatca accggattgt tccgctatcg ggacgatcca aaaatccgag
     4501 aaggtgcatg agcgtggatt taccggaacc tgacggcccc ataatcgcga gaaactctcc
     4561 ggcctggatc gtgagagaaa cttcctggag cgcgcgaact tcaacttcac ctattttgta
     4621 gcttttggaa atccggtcga tggctagcag gctcatgatc gtttttgtga tccgcccgac
     4681 cgggaggatg aggatgggct gagcggatta ccgttttttt cctgccgctt ccttacaatg
     4741 gcaatctcct gtccttcttt caaaccctcc ttgatttcgg tcatacgccc gtccgtttcg
     4801 cctatcttga ccgctaccgg ttccggcttt cctccaaatc ctttccggta aaccgcgacg
     4861 ctcgccccag acggattggg aaactccttg ggccgcgccg tcaacgcttc ggatggaacc
     4921 agcagaacgt cctttatttc tttgacaatg aatgtcacgc tggccgacat cccgctacga
     4981 aaagacggtg gctcttcttc cggaaccaca tcgatcgcgt aaatgttgac gttgttttcg
     5041 agtcggcttt caaacgcgat atgatccact ctcccggtaa tcttttcgct ccggtaggcg
     5101 tccagtatga tctcagcgga ctgtccaagg ctgatgagcg cgagatctgt ctcatccacc
     5161 agggattcca caatgagacg gtccgaaagg accagaacag gatccgcggt cgtcaccgtc
     5221 tgtccgggct caacggaacg gacaatgata gttccgtcaa gcggcgcaag gagcggcgcg
     5281 gggcggtagg cttcttccca ccgtctcacc gcttcccctc cccgggaacg tgccgcgtca
     5341 agtaaagcgg cccgctcaat cgaactcatt cgagctaaca cttgcccctg ggtcacctca
     5401 tctccctcgc gcaccaatac ctcgtcaatc cgtcccgcga tcggcgcctt gatttcgacc
     5461 cggttgtaag gcttcaccgt tccggtcgcg gtcacggtca gagataaatt cccccgcgtt
     5521 gttttctcga tacgaaccac ctgcaggtcg cttcccggag ctccgcatga cgaaaaaatt
     5581 ttcgctccca ataaaattcc ggtgatcacg ctcgcgacct ttgccgaccg tggcaagtta
     5641 ctgattttga atttcatcgt aagcctcctc gagtgtgacg ccccggattt ttgcccactg
     5701 ggattgctga atgaaaacgt tggcgagact ttcgacatat tctttttcgg cgttggcgtt
     5761 ctcctgttcc gccgtatcaa aatccagaaa gttcaccaaa cctgtcgcgt attccgtacg
     5821 aatgatttca gctctttccc tcgcagcttc caggaatttc tttcggacct gtaccacttc
     5881 taccgcgtca atgaaaggcg cgcgtgccgc ggccagttgc gcaacggttt cgtcacgggc
     5941 gctacgaacc gcgttttcgg ccgcgtcgta atcggctttc gcttttcgga tggccgccac
     6001 atttttccca ccctcaaaaa acggcatggt gacactcacc ccgatcgagc tttgatcttt
     6061 taaatgagac gccctgtctc ccgagtaacc atattcatag gttccatcta cctgtggcca
     6121 gactgaagac tgcgccgaca tcatttccgc cttggcggat tcggcaacct tcagaagccg
     6181 cagaacggcg ggcgtttcat cggcgaggcc cgcgtaatct tcttccggca attttacaaa
     6241 cttcgtcatt ttttcaagat catcatccac tcgtaacgga aggacaaatt ccccgccgct
     6301 ttcccgggca agacgaagag cttccttttc aagctgtcgt tccgtctgtc tgacgtcaaa
     6361 agccgcttgt tccgcgatgg cctccgcgcg aagagctgat ccaagatttt cccggccagc
     6421 ttcgtaacgg agccgaacca tttccgcgtt ttgttttctt ctttcggaaa tcagccggtt
     6481 cgtctcaaac agtttttcaa gacggatcac attgatatag gatgttctca gccgatgaca
     6541 gatgtccgcg ctcatctcat tgtacgcgaa ccgttccgcc tcaagatctt tccgggcgcc
     6601 aagccacttt cctgttgttc ctaatccgtc aaaaaacgac tgtcgggcgc taatgtccag
     6661 gtttaatcga tccgaggtcg ggccactcgt cgtccgggaa gcgcttttgt gaacgcctcc
     6721 atcaaccgcc ggcagataat ctccaaaagc aatgtcccgg ctcctggcct ttgattccac
     6781 tctggccctg gcttcccgaa gatccgggtt gttggcaagc gcttgccgaa ccagggcgac
     6841 ccaggttacc gctttgggct ccgactgtcg ctcaatctct gccttcggca agtgcacggt
     6901 gagacatccg cccaaaagaa acgcgactcc aagaactgaa acgtgttttt caaacatgct
     6961 cggctttttc ccgtccgttt tctaacgtcc ctcttaaata ttcaatatat agaccctgaa
     7021 aagccttcta aagttcccgt ttttttccgt atccgtaatt atgaacgagg tctgccggac
     7081 aatttaaaac ccgcaaacac tctttgcccg agcagggtcg gaaaaatcgc ccttgccaac
     7141 aaggtgactt caggatcgat tcttcggata acccacaggt tgagttaaaa tgattctttg
     7201 ccgtggttga agcttcatcg ttttggcgag ctgatcccgg tcaatcgttc caagcaccac
     7261 cgttgaaaga ccttccgagg cgcaaaaaag ataaacgttc tggctaacat agcccgtatc
     7321 agtcgcgctg taaaacaagg cgtcttcttc ggagccgcca cggaactttc cagtgttcga
     7381 gacaaagata agtcccaccg gcgcatcctg cacaaaaacc tgtcttccga tccgtgaccg
     7441 gatatcttcc ggtatcacca ccgtcaacgc gtgttctttc gcgtcataaa gataaagcgc
     7501 ttcttttttg gcgacgtaaa tttcgatttc ttgcgtgttt ttcgcggaag gggaggtcct
     7561 ccggccgtcc gggcgattca ccccgttcgc ggcccagaga agattggaaa gaacttgcgg
     7621 cggtaattca tccggtcgaa attcccgtga acttttccgt tctgataacg cttgcatgag
     7681 gggctttcct ccctccgtgt caggtggcag taatctcata tttgtaacct ccgcggtgaa
     7741 accagggtct tgaaaaatcg tcgtgaataa aggcaacatc aggatgatca caaaaagccg
     7801 tttcattttt tctcaagcct ccttttttcc ataacttttg aaagctttta aaagcgcgcg
     7861 aatatcagct tccgtatgtg aagcgctaac cgtaatgcga agccttgctt ttcccttggc
     7921 gaccgtggga taacgtatcg ctggaatgaa gactccctct ttcagaattg actgaaaagc
     7981 ctctaacgcg cgcttttcat ttcctatcat taccggcaag atagctgact caggtttccc
     8041 caaaacaaac cccgcctcgg acaacccgac gtgtattgat cggatattat cccataactt
     8101 tttcctcaag gccgtttctt tttcaatcac cgagagggat tccagggccg acgcgctcaa
     8161 aaccggagga agcgccgtgg tgaagataaa cggccgggaa aaattaacca gataatcgac
     8221 gtaagggtca tcggccgcga caaatccgcc cagtccgccg atcgctttgg aaagtgtccc
     8281 cataattatg tcgatctcct ttgagaattt ctcggtcgcc ccgtgaccgt gcttgccaat
     8341 cacccccatc ccgtgagcat catccaccac aagcaacgct ttatattttt tcttaagccg
     8401 gacaagctcc ttcaaatccg cgaggtcgcc gtccatgctg aaaacacttt ccgtcaggag
     8461 aatttttcgc cgccccacgg acttatggag aatagcctca catttttcat actttttgtg
     8521 cggaaaaacc ctcagttccg cctgggagag ccgagctccg tccaccagag acgcgtggca
     8581 aagtttgtcc ataatgatcg tgtcaccttc cctggcaaac gcggtcagga tcccgaggtt
     8641 agcgaggtat cccgaaccaa aaacaagcgc gcgttccttg ttttttaacc gcgctatttt
     8701 tttttcgaga gcttcatggg cggtcgtgct ccccgaaacg agccttgcgg ccccggcacc
     8761 cactccgtat tttttcaggg cctgttccgc ggcccggatc acgcggggat gccgggaaag
     8821 tcccagataa tcattcccgc aaaacagagt gatttttttc ccccgccatt ccgcatgaat
     8881 cccgtccgaa ttcttcaaaa ctcggatttg tcgatagagg ttattttttt ttaggtcgct
     8941 gagttcatct ctatttgctt gtttcatgtt caagtttgaa tcctcaaatt tttcgcagga
     9001 gtactgccat ggcatgaccg ccaaacccca tcgaaacctt gagtgagacg gtcatctttt
     9061 tttctttcgc tttaaggggc gagtaatcca gatcacaagc cggatctttc tcttcaagat
     9121 tgagggtggg aggaataaag ctttctttca ttgccagcaa agttgccgcg atctcaaacg
     9181 cgccagtggc cccaagcatg tgtccggtcg ccgccttggt tgaactcatg ggtatcccat
     9241 acgcttcctc tcccagcgcc accttcattt ccgcggtctc atacaaatcc ccgtgcgtcg
     9301 tgccagttcc gtgaagatta atatagtcga tttcgtgagg acggatggcg gatcgttcca
     9361 tcaggttttt aacagctctc gacaatgcgt gatcatcccg atcgaacaaa atcgcttgcc
     9421 tcgagtcctg ggcgcaagca gattccacga tttcaccgta aacacggatt cccctcgcgc
     9481 gcgcactttc aagggtttcc agaaaaagta ccgccgcccc ctctcccacc aaaaaaccgt
     9541 cccgtcgctg atcaaaaggg cggatctcct gttttgcgag cgctctcata ttgttgtaac
     9601 ccgctagcat cagaggaact atggacgaat ccgcggcgcc ggcaaggcag tattccgcct
     9661 ggccttcctg aaccattcga aagcccgcga tcgtcgcggc gacgcccgtc gcgcaagcgg
     9721 caatataaca ttttgccggc cccttgatct tcatacgccg ggcaatccat tgatctccaa
     9781 aattaggaac tgaactggta tagatacgag cccccataat cgcgctggga ttttttaaaa
     9841 accggtcgcg aaaccgatcc atggtatgca ctccgccttt ggacgagcta atcgaaagac
     9901 ccaccacata aggatcgaca gaagctacgt caaatttcgc gtcctgaacc gcgctttctg
     9961 ccgccgcgac cgcatactgg ataaaaggat cgaggatgtg tgttccatag tgacgcggat
    10021 tgaaattttt gacacggaaa acgggcggaa attgatatcc aaagacatca gagacaagct
    10081 catcctcttt acccgaaatc ccgtttcgga ggttttgagt tacatcttca actgtattac
    10141 caatgggcga gagaaaacct atacctgtca ctacgacgcg tcgtttcata agtgggatcc
    10201 attataacgg gtggtcacca accgtccact tatcttataa caaaccagct ttcactaaaa
    10261 agctggttta accattttgt tgaagtgtgc ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnccccttaa caaaccagct ttcactaaaa agctggttta accattttgt
    10441 tgaagtgtgc ggtcggagac cgtccacttc tccccttaac aaaccagctt tcactaaaaa
    10501 gctggtttaa ccattttgtt gaagtggacg ggattcaaaa tggaacactt gacccgtcac
    10561 tcgactcaca agatctgagg agaggtatat caaaaaattc gccgcctcca ccgggtccgc
    10621 caaggtttcc aaagggcttc gtcccagggc ccgttgaatc actttttctg aaagcgcggc
    10681 cgtcagtcgg gatctcataa atcccgggtt aacggcgttc accaggatct ttttggggcc
    10741 cagttccctg gcaagcgcca gggtcatcgc gatcaacccg cctttggccg ccgcgtaatt
    10801 agacgccccc gcaagacctt ccgttccggc gcgactgaca atcatgaaaa ttttgcttaa
    10861 agaaaaatga gagttttttg tacgggcgga gcgcgacccc cttttttcaa ggagcggcaa
    10921 gagtttttga gtgaggtaga agggtgcttt aaggttcgtt gcgagaaccc gatcccattc
    10981 ttcttcggtc atcttctcca agaggtgatc tgccgctacc gcggcgttat gaatcagaat
    11041 gtccagggtt ttgttctccc gacaaaaagc ttcgagaaaa gagtcgatcg cttcggtcga
    11101 agaaagatca gctttgtgtt ttttcgcgcc gatcgtttca agctccttgg cgcgttcagc
    11161 cgcgctgaaa aacgtaaaat ggatctcggc tccatgagct attgcctgtt ttacaaaagc
    11221 ggtcccgagc tcgcttgttc cgccagtaat cagaattttt tttccggaaa gatattgatt
    11281 caaggtttac gcggtttggg caacggccga ctcgcgctca acgatatagc cggcccgttt
    11341 aagcagggca aagtcttcag cgatcgggtt cccgcaagtg gtcagatacc cgccgacaac
    11401 aagtccgttc gcgccaccct tcaaagcttt ctccaggaaa aagattccga gattgagctc
    11461 tttacctccc gcgagcttga tgttcgcgtg gggatggata aaccggtaaa tcgcgatcat
    11521 tttaatcgcc tcggccgggt cgatcggcgg ttgtccggcc aggggagttc cgggacgagg
    11581 attcaaaata ttgactggga aacaatgggg ggcatatctt tttaattcaa aagcgagttt
    11641 gagtctgtct tcagggcttt ctcccatccc aaaaatcccc cccgaacaaa tttctacatc
    11701 gttctttttt aaaaaattga gcgtttccat gcgctcatca tacgtgtgcg tggatacaat
    11761 ctcaggataa aactcacggg agcattgaag attatgatta aagcgccgca atccgacttt
    11821 tttcagctcc gagatctgct cctccgtgag attgcctaaa gaaccgtcaa ttcccagacc
    11881 cgtttcttcg gaaagtagct gaaaggtctt ccggacttgc tcgaactctc ccgcgctaag
    11941 ggtctcaccg cttgtgacaa cgcaaaagtt tcgcgccccg ttttcttcac atcgcttcgc
    12001 ggcctcgacg attttttccg gactcatcat gggatatcgc tggactccgg tgtcatactt
    12061 cgcggattgg gaacaaaatt tgcaatcttc accacagaga tagcttttgg cgttgattaa
    12121 agcgcagagg tcgggccgag aatccttaaa atggagagtc aattcatggg ctgaactacg
    12181 taaaaaatcc agatcgtcaa cggcgcgtat cgcggcaaga gagagggcct cttgatatga
    12241 aatttcacct ccggaaagaa ccttttcctt cagtttctgg attaacgttt tcgcggaact
    12301 cgcgcgggga tctcgaaaca ttgcgggtct ctggttctca ggcctgggca aaaacttcgc
    12361 gggctacaat tcctacgaca cccagcgcga cggcattttc accgaaggcc gaggggatga
    12421 tcttgaccgg cgtcacggct tcttcgaccg accattcctt aacgctactc tttatagaat
    12481 caagaagcgg cgccccgcaa tcttcaattc cccctccgat cacgaccact tccggattca
    12541 gcaggttaac gataaaagct accttcttcc cgagttcctg tcccgcctgc tcggcaactt
    12601 tcatcgcgag agcgtccttt tccttgatgg cgcgaacaat gtcgcgaaaa tgaatttttt
    12661 cgagatctcc atggacgtaa tccttgatca ccgagggttc acccttttcg atttgttctt
    12721 tcgcgtgacg agccatcccg atatccattt cccagcggcc caattgcgtc gctatctctt
    12781 gcgtgtattc cttgctcgcg tgaatgctgg taatcccgag ttcacccgcg gcccccgtgg
    12841 ccccccggta aatttcctca ttgatgagaa tgccgcatcc caccccagaa aacatgtaca
    12901 ccatgttccg gacgtcccgg tcaagtccca gccacttctc tcccaaaaca gccgcgttgg
    12961 cgtcgttttc aacaaaggcg ggaatgccga ggcgtttttc gaaagtgtct tttattgaag
    13021 tgcaaaccga aatgtcttta tcgccaaggc tctggggcca gcgaatcgtc cggccccgct
    13081 catcaataat tcccgggatg ccaactccca cgcccacgat tttggttttg tcaatttccg
    13141 ctttttctat gatttcggcg gcaagatcga tcatctgctg gatgaccttt tcggagtttt
    13201 cgggaagcct ttccctcttg agctccgtga caacgttaat ttcaagatcc accagcactc
    13261 ccaccatgct catcatgttg agtccgacgc cgacgatata accagccttg gggttcagct
    13321 caaccaggac gggtttccga cctccggtcg actcatcaag atcgccttcc accacaagtc
    13381 cttttttaat gtagtgatta acataattag agaccgtgac gatattgagc tcggttaatt
    13441 ttgaaatgtc ggtcctcgaa atcggtccgt ttttccgaat gacctcaaga atgctaagat
    13501 ttttcctctc tttgtccgtg agcgcccgat ctttaagcat ttgcttaatc atcataaggt
    13561 ctctcctgtt ttaggatttc tccagttttt ctatttttta taaccccaga tcttatcgct
    13621 atataagtcc gacaaacgcc actcattcta acctgaaaaa atcctttttt aaactcttgt
    13681 tttggcaaaa caagattttt atttacgatc tttaaaaaac ctttctccct caaaaacgct
    13741 ctgggctttt tgggcgatag ggtctttcat gaaaaagttc tggattattc cttttttaag
    13801 ataattcgca atcgctatca ggctcatgcc ctgatccaga gaaagatact gattcgtaac
    13861 gagatcattt ttcaggttaa tgctgtcata aaaaccatat tctccgtaaa tgtcataaag
    13921 ttccagcatt tttctaagat ttttgacaac gtctccgggc aaagtttcca gggccaggag
    13981 cgcggcatag ggggcgataa ttgctttttc cggataacct ttggttccca actttttaat
    14041 cccgtattcc ccgtaaaacc atcttcgtcc atttgaaacc gccgctggag aaatacccca
    14101 cacaggatat tccttctcgt ttaaagcgta gtcgcgatgg atttcagtcg cgactctatt
    14161 gttcctcccg aaactttccg gggataactt gtcttcagga ataatcaggg tcggcatcaa
    14221 aaactcaaaa agagagcctc cccagcttgg cacaacctct ttggaatcca ccgtataata
    14281 cccttggaaa tattccactc cttcctttgt aacccattgg ccggaaggtt tctgcgtctg
    14341 ccaatcccag gcgtccgggg cagtcctgaa aagataccac caatgctctt tcggaatgtc
    14401 tcccttccca atccctatga agctgatgat cctggcctca ctgatcatca tcccgtaatg
    14461 atacggggtc aaccctgcgc gttccaggtc gtagccaatc gcgatgtgat tcgtatcggg
    14521 atcataaaac tcctcaaaat tcatttcctc gaggattttt gaggctcttt tagcttcggc
    14581 gcccgaaaac gcttgtcgcg tcactatcaa tgccgccgcg agccacgcat tatcaacgct
    14641 ggaaataaat ttccggttaa tggaaagctt gttggtttcg taaaaattat aaaaaaaacc
    14701 cctccagcgc ggcaactttt gaagcgcatc cagcacaaga tgggcccgtt cctgcgctga
    14761 ctctttcgag atgtaaccca gctcccgcgc cgcgaccgtg caaataaggt ccatggcgat
    14821 attggtcggc gacgtataaa ctcccaccat cgggaaatcg cctactttaa tgtggtcaag
    14881 gacgaggtga ttttccttgt tcgcggcctc ctcgaaataa cgccaggtgt cttgccctat
    14941 cgcgttaaga aaatccgaat ccttggtcat cgataaaagc cgttgtttgc cggacaagta
    15001 tttgacaacc ttcggaaaat ccttgatgtt gtccagcacg ctttcaaatc caacttcgtt
    15061 atggccaaaa aagacaatgt cgtccagcca gagattcgcc ttggatctct tatgccgccc
    15121 caacaccgtg atcgataacc tgtccaactg gtcaaagtcc agccctttga agtactcgac
    15181 aggaagaatg gcttcctgcc atttttccga tttcgcgcat gtttccgtaa tgtctctcgt
    15241 tacagatcga cctgtccaat ccgtcagact aacccgaatt cttccgttaa atccgtgttt
    15301 tggattgggc ttcagcttgc atttcatggc aaggaaccgg gccttgctca tatccagttt
    15361 tcccaaggat gtggaaaggg tgaagtgttg acgcggatca agctcaaaca agattttaag
    15421 cgactggccg gggcgcatct cccggccatc ttcccgggag tgttccatgg taacgctacg
    15481 ggcttttccg ttctcgagcc gccaatgatt atgcaggcga tttttccatt ccttatagtt
    15541 ggcaaaatcg tctatgacta aaaactgggg agaggtaaga agagatttat tcggaggaac
    15601 ggtgttataa attttggtaa tttccagtgt ttcatcagcg ccacactgaa ttcggccgcg
    15661 ggcattattc gcggagacag aggaatcctc tctctgttcg ttcctgaccg ccgcgaaaag
    15721 gaacgccggg atcaacagga aaaaacaccc cataaaaaaa gcgcttttca gagttttgtc
    15781 caactccaaa aagcgctttc gttgcgattg cgtctgagtt tctgtcatcc gatcacaacc
    15841 tcaactcggt ggttttgccc gtcctgaaaa tccggaatca ggtctccggg ctgttttttt
    15901 ccatctactt tcagttcttt gaccccctgt ccctgaccgt tgtttgttac cgagatatgg
    15961 taagttgtcc cgcggaaaag tcttttcatt ttaaatcccg gccaagcttt cggaataacg
    16021 gggtctaccc gcaatccctt tgaggtcgga cggatgccga gaagccagtt caataccacc
    16081 acggcatacc acgcgccgga tccagtgtac caagtccatc ctccacgacc gtagttcggc
    16141 gaatcgggcc cgtcgacgtt ccccggggtc acgtaaggct cagaaaaata aatctcgggc
    16201 ttcaatcccc gcaacaccgg attaaaactt ttgtaaagtt cgtaggcctg ctcggcgttt
    16261 cgcataaggc attcagcctg aatggcccag gttccggcgt gagtgtaaag ccccccgttt
    16321 tcacgaacgg atggcgcgta acgcgtaatg tatccaatgg tgggatccgt tttgttatat
    16381 cccggcgtca gaagcagggg tccgtatttt ttgtagagcc acttcttggc cgacgccatg
    16441 gctgttttcg cccgttctgg agtcgccgtc ccgcaaatca cggcccatgt ttgggcattc
    16501 agaaaaattt ttccgcgctc acagttttta ctgccgagaa tctcaccatt atctttcgtg
    16561 gcgcgccagt accactctcc gtcccaggca tactggttga tcgcctcctt caataattca
    16621 gcccggcgaa gatagtcttt ggctttggcg gtatcgcctc tttgtttcat aaggggcgcg
    16681 atcctgctta aaattccgta aagaaaatgg ccgagccaaa tcgtttcacc tttccatttg
    16741 tttccaacgt ggctcaaccc atcattccag tcacattccc cgatcagcgg taatccccgt
    16801 gatgaaaacc tcgaaagagc tttatcaata gcgcggagaa ggtgttcata aagtggcgct
    16861 gaagtaacct ttttttcggg atctttcggg tccggaagaa acttcacgtc aatgtcaaga
    16921 atcgcgtggt cgtccgtctc ctcgaggtag ttcagcgtta taaaatccaa ccagagaagg
    16981 tcgtctgaag aatgagtgat cgctccgata ttcgttccgg ggtgccacca atggtaaacg
    17041 gttccgtcgg ggaactgttg ttcggcatgc aaaaggattt gttttttagc gagttccggg
    17101 cgagttgaaa aaaagatctg acaatcctgc agttggtcgc ggaatccata gcctccgctt
    17161 gattgatagt aggcgcattt cgcccaaagc ctcccggaaa tcgcttgata tttcagccac
    17221 gtgttggtca tgaaattcaa cgattcatcc ggcgtttcaa cctcggtcga gtgaatcaaa
    17281 gtatcccaga aagccttgac cttgttgagt tcacggtccg ccattccggg agtttgatat
    17341 ttcttgataa ttcttacggc ctcttcccgg gttggagttg aacccagggt aaaaacgatg
    17401 gtcttcgatt gcttgggccc gagcgtaaca tccacctgaa ggctcgcggc cgcgtctccc
    17461 cacttccctt cggagttcct gcattttcca gcctccacgg ccgcgggcgc atgctgttcg
    17521 ccgtaacgtc caagaaaggc ttctttatcc ccgtcgtaag ccacgggctt gggaaacgcg
    17581 ccatgaaatc cccggagggg cttttcggtc ataccggtcg atatatatcc cggaacaaga
    17641 ggggcgcgct tttcgccgta aaggcatccg ttttttttat cgatccaaac ttcaataaag
    17701 gtcttatgaa attcgcgatg ggtatccgcg gcattcccca agcaccagtc aaaataggaa
    17761 aaaacactca ggcggcgttt tttttccgtc tcgttggtaa gaactatttt ccagacctca
    17821 acaggttcag tcatgtccac gaagagtgtc ttctcaaatt tgataccatt cttcttcgtt
    17881 gtggtgacgg tatagccctg tccatgacgc acttcaaaaa aatcatactt cgtcatgcac
    17941 ggcttccagg tgcccgacca gtatttcccg ttgtctttgt cacgaagaaa aatatatttc
    18001 ccccagttgt cgcgtatcag atcctggtcc caccgggtga tgcgagacag atttgagttg
    18061 ccccgccacg aaaaccccga tccggtttga gtttcaatgg tgccgtagtc cccgtttgaa
    18121 atcacgttca cccagggacg cggtgtatcc ggacgggtga tgacatattc ccttccatcc
    18181 gccgtgaaat aaccatagga ggatttaaac ttttgctgct cctttgttgt cttttggctt
    18241 aaggcgacac gtttcttttt tgattttttt tgactcatag gggaagatcg ctcctttaac
    18301 gctagatcga atccgtcaat gattaggaat tcagcagttg attgatttcg gccgttactt
    18361 ctttaacaac ctgctccgcg cgtttttttc cgttaaaaac tgtttcgagc tttggcttca
    18421 aaattttttc ctcaatctct cgccactggg gatgaaaggg actgaagacc acgtgctgaa
    18481 ccgcgtcgtt caacattttt ttgttcgcgg gaggctgggg gtctttggca aaagctaaac
    18541 cgccggccac ttcttttctg gccggttgcg cgaggccacg cttcgcgaaa agctcctgtc
    18601 ctttaggccc cgtcagcgct ttaatgacct cccacgcttc cttcttgttt ttggatgatt
    18661 tcaggatgca atagcctgtc ccgcctgtcc caaacgcccg ctttccttcg gcgtttttgg
    18721 gaaacatcac gacatcccag ctgatgttgt aattcctaaa aagaggtgtc tcccaaatac
    18781 cggaaagaaa catcgcgatc cggtcgttcg caaacattcg atccgccccc atgccaaaat
    18841 tcataaaggc taccggtgtc ggcatcgccc cataaagatt gctcatgtcc gcataaaact
    18901 gaagcccttc catagtttta ggatcgtcca gaagcgttcg ggtaggattt ttcacgtcat
    18961 caaccaacgc ccccccgttc ccgtagacaa aattctgcca ggcccaaccg taaaacccgt
    19021 actgggtcac ccggccactt tcatcttttt tggtcagctc ccgcgaaagc ctcaaaagat
    19081 cattccaatt ccagtcatct gtgggataag ggattccggc agcatcaaaa agttttttat
    19141 tgtaatagac acaggcaaac ggcgcgatgt cgcgcggcag ggctaaaagt tttccgtcat
    19201 aggtaaatcg atcgataatc tgggggaaaa aatcctcttt tttaaaaccg gcggtgtctt
    19261 cttccatata aggggtcagg tcttcaagga cgcctttcga cgcgaacgtc acgaaatagt
    19321 ccacctcggt cgcgataatg tcgggagccg ctccgccggc gatccgggtt aaaattttac
    19381 tggtataacc cgtgtagggc gtgtgttcaa aaacaatctt gatggtggga tgctcggcgt
    19441 gccagtcgga aagagcttgg gtaataatgt caatttcttc cggtgttccc caaaagctta
    19501 ccttgaccgg tatttttcca tctccggaaa gttcagcgcc acagccgacc aaaaaaaagc
    19561 ttgttaaaag gatggaataa acgaataaac ttttcaccca tcttttgttc atgtcggcgg
    19621 ttccggattg aggttttgtt ttggtaatcg gaaaaactga cgcgtttcgc ggattataac
    19681 atggtctttt ttgaagtcaa cgatcagccc ctgtcttttt acccgttggg agcaagattt
    19741 attttttgga gaattcggtc cagatcatca agggacgagt aatgtatctc gatacgtcct
    19801 tctttttgat tttttcgcgg ataaatttta acctgcgctc ccaaatgatg aaccaagcga
    19861 ttctccaaat ccacgatctc cggacttaga tgccttgcgg tctttgcttt gcgtttgtgc
    19921 gcattgcttc gattgaccat taattcgacc tggcgaaccg aaagattttc ttcgacaatt
    19981 cgcttggcca tttgccgttg atgctccggg gttaaaagcc ctaataaggc gcgggcgtgc
    20041 ccggcagata gttgtccggt cactatatag gttttaactt cctcgggaag tctcagcaag
    20101 cgtatcgtat tagttaccgt ggttcggttt tttcccactc gtttggcgac atcttcctgt
    20161 gagagagctc tcgattccat gatcttttgg taagcttctg cttcttcaag aggattgaga
    20221 tcctgcctct ggatattctc gataagcgcc cattccaaaa aatcctcgga agccacatcc
    20281 ttaagaacgg cgggcacttg cgtcaatcca catacctttg ccgcgcggaa ccgccgctcg
    20341 ccgcaaatta actgatactg attctgtccc gtcattttca caatgacggg ttgaagtatc
    20401 cctttttccc ggattgaatt cgcaagttcg tctattccaa ccggatcaaa atgctggcgc
    20461 ggctgatttt cgttcggagt gatctgctct acaggaatta accgaaaagc gccttgaggt
    20521 gccggggaaa ccgccagact tggggcggct ggcgcggatg acgttacatg cgcggaagct
    20581 ggtatatttt tttccatctc tttcccttgt acataactat cagggataag agcattcaat
    20641 cctcttccaa gtgccttttt catgagcttc tccttaatgt tatttcacta aatgattata
    20701 aataaaaggg ttatgctttc tcatctcccg gaacaacatt ttccgagtga ttattaacgt
    20761 tggcaagatc tgtgttttcc gggtttgcgt tttccgattt gttactttgt ggtattgaac
    20821 cttcaagaac tggattctca gcaccatccg tcattttttg ttcttgtgct agtcgaatgc
    20881 ggtcatcctc agtaccaaac catttcaaaa attcggcacc caattccata tacgcctgcg
    20941 agccccggtt atgagtatca taaatcaccg ccggctttcc aaagcttgga gcttccgaca
    21001 atttcaccga ccggggaatc accgaattaa aaaccttctc tttaaaaaat tcccggactt
    21061 cgtcaatcac ctgctttgtg aggttggttt ggaagttcgc cattgtaagt acaactccac
    21121 caatcattag atcggggtta agcccttttt tcactaactc atatgtctta agaagagata
    21181 caagtccctc gagggcatag tattcgcact gaagcggtat aataacgtaa ttgcttgctg
    21241 ttaatgcgtt tatgcttatc aagccaaggg aaggcgggca atcaaggaat ataaaatcat
    21301 aatcattttt cactttttcg agttgcgtct tgagaacaaa ttctcttttt tcctggttaa
    21361 tcaattgaat ttccgcacct gcaagatctg agtttgctgt aatagcgtag aggtttttta
    21421 tcgacgtttc tttgatggct gatataggat ccgagttatc cacaagaagt tgatatgctg
    21481 tgaattcaga gttgttttta tctaacccta tgccactagt agtgttacct tgagcatcaa
    21541 gatctattaa aagaaccttt ttttctcttc cagatagaac tgttgctata tttatgactg
    21601 aagttgtctt tccaacgcca cctttttgat taaaaaagga aaatatagcg cccataaaac
    21661 ctcctcatgt ttcacgtgaa actattaatt tttattattt atcggaataa cgacgagaca
    21721 gtttatattt attttcgcaa gaactcaata tttttttgaa gtcacacttt cttctcgatt
    21781 tctatacaag gcatagaaaa aaagattgct ggagattttc gcttacgagc tatttgttcg
    21841 aaacaaaact attttacttt gtggttgaat aacaaaaaaa tctatccata tcgcatgggc
    21901 atggttttat tcaccgaata taactcttgg gcgcattgtt tcacatgaaa cagtaaaaaa
    21961 catatagcat tgcaaaacgt tgaaatgtat tgggtaatag attgtctttt cgctaatatg
    22021 tcaagttcta taggtgggtt cttaagtgat tgcctgtgag ctttttctaa tcgttaggaa
    22081 tgttaaaaga tgatgctttc acctaaaaca aactaatgtc gcttataaaa tgtaaaacag
    22141 agattttcaa aatcaaagtc tggaagtttt aagaattttt tttggcagct ttttcaatgt
    22201 aaatcgccaa aagagaaatg tcacagcttg aaattcctgg tattcgcgcg gcctgcccaa
    22261 gagattccgg ttttattttt ttaagctttt cgcgggcttc tcggctgatg ttggagagat
    22321 cgtcgtaaca aatcgtttgg gggatttttc ttttttcgat ttttgtaaat ttttttacgt
    22381 cgcttatttg ccgggaaata tagccttcat atttaatttc agtttcaaca aaatgcttct
    22441 cattttctgt taatttagat gcgtgtaacc ctttctctat caacaactta tagctcattt
    22501 ctggtttttt gatctgttgg taggcggtat cctgtccaac cctttttatg cgaagttttt
    22561 ttacatcgtc tttgattcgt tcgcaacgct ctaaaaattt ttgatattct tcatttttta
    22621 caagccctat ctcatacccc aaacgcataa gacgcatatc cgcattatct tgtctcaaca
    22681 aaagacgaaa ttcggcgcgg gacgtaaaca tccggtaagg ttcatttgtt ccgcaggtca
    22741 ctaaatcgtc tatcatcacg ccgatgtacg cttgcgtcct cgcgagagta aattcctttt
    22801 caccgcgaag ttttttgacg acattgatgc ccgcgatcaa cccttgtcca gccgcttcct
    22861 cataaccgga cgtacaatta atttgcccag ccaaaaacag attttttatt tttttgtttt
    22921 caagactgtg tttttgctgg gttgggggag cggcatcata ctcaatcgca tagccaaacc
    22981 tgagtatttc cgcgttttca agccctcgta ctttatggag agccgtatcc tggacgtctt
    23041 tggggaggga ggttgagaag ccgttcacgt aaatttcatc cgtatcccgt ccctcaggct
    23101 caagatagat ctggtgtcgt tctttatccg cgaatttaac gatcttgtcc tcaatggacg
    23161 gacaataccg cggcccgatg ccaacgattc ttccggcata aagaggggag cgggtcaaat
    23221 ttttccggag tatttcatga acctcgctgt tcgtatgagt caaccagcag gacatttgct
    23281 cgacttcacg atgggttgtt cggtgtgaaa aaaatggcgg gggttcgtct cccttttgtt
    23341 cttcggtcag atcccatctg atgcttgccc ggtggagacg tggcggggtg ccggtcttaa
    23401 accggaccag ttctatgcca tgtctcatga gcgaatccga caggccaatc gctggaggct
    23461 cctcacacct tccgccaggg tagtgttctt ctccgatatg aattaatccc tggaggaaag
    23521 ttcccgtggt aagaatgacg tgtttgcctg aaaaagtttc tcctgctttg gttcgaacgc
    23581 cataacaggt tccgtcccgg acgagaatct cggtcacttc tccctgtttc aaggtcaggt
    23641 ttttttcagc catcagcgcg cgcaacatgt agcttt
//
LOCUS       DAOSIO010000003        21121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_2759_length_21121_cov_4.900171, whole genome shotgun sequence.
ACCESSION   DAOSIO010000003 DAOSIO010000000
VERSION     DAOSIO010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 21121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21121
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2759_length_21121_cov_4.900171"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(553..2193)
                     /locus_tag="PK590_00275"
     CDS             complement(553..2193)
                     /locus_tag="PK590_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23065.1"
                     /translation="MTNERLMKKGTVFLVGALLAIMLPGTLCAKSAKFKGKATHLYKD
                     VFDQNVYYEGVNQLDFGRWGRKLFDREIPAANINIFDEVPDSAFFTNRHARKRLSAEE
                     LEKGYSETEGPDLSAPMTVTKGKQEGLHPGFFIRDARGDAYCLKFDAEGYLELATAAE
                     VIASRFYYAIGYNVPQYTMFRFRPEDIVAGENAYTYDDTGFRKKMTPDLLDQYLSFLP
                     ITEEGEYLASASKIVPGRNAGYFSFYSRRKDDPEDTINHRDRREIRAISVFSMWLNNY
                     DVREDNTLDMEVVENGRPVIKHYLIDFNSALGAAHGGPKPPMFGHEYMIDYGETAKAF
                     LSLGLWEKPWQKKWRVVDERPYESPAVGYFSNEGFDPESHKVQLPYETFRVLTKADGF
                     WAAKIMKSFTDEDVRAMVKAGRYSNAEDEDYIAKTLIERRDMIARYWFSQTTPLDQFE
                     LSGNVLRFRDLALHYGWGDPDAIDYTAEVFAGSKRRKPDEILTSQEPVFSLNPRYISP
                     NRTVELRIRVSRRGDEDPSPYIKIILDEGGVRQIRHED"
     gene            complement(2207..3520)
                     /locus_tag="PK590_00280"
     CDS             complement(2207..3520)
                     /locus_tag="PK590_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOG23066.1"
                     /translation="MINKHKKLLTLVLALLFFAGSSYAQDIYAPVYPHEPLKAKNLKD
                     PVSALLEIPFRLVRWPIDQSLVYIGKHHIDKKTFHIYDKIKGYGVTPHIAFTDSAILP
                     LVGLELDLMTMTRKKVEHPDVVANASVRYAMHNLFIVDSKVGVNRIAGTGLYTFGNFN
                     FDHRSREPFYGIGPRTSLGDSSSIRMETVYLAWNAGYEFSPSLNVLGTFAFKENQIKH
                     RAHDGKGDMTEVFAGQDIPGIHGGEELNWKMELVRDTRDTKDEASKGSYQKLSVKLTE
                     GVDSTKARFLTYQLDAAKYFSLKSPRRVLAIRAFGEHNDEINHGTIPFYDMARLGGMG
                     SSQRTSVAHRAFVYNRFYGESAILFSLEYRYAIWEYRDFRLNASVFWDEGQVFNEFNK
                     FKFSDFRESYGLGFYLSYAKHTLLNFTVAHGDEGTRVYVKNRVPF"
     gene            complement(3517..4050)
                     /locus_tag="PK590_00285"
     CDS             complement(3517..4050)
                     /locus_tag="PK590_00285"
                     /inference="COORDINATES: protein motif:HMM:NF036460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5329 family protein"
                     /protein_id="HOG23067.1"
                     /translation="MLKKVFKEKSNKAVVLLSVVLLGLILAEPRAVFPEEETEAQDVC
                     MLEKLAIPEESPAFRRLLKTTPATSEYQRARIDFLIERVGKSDFDFIWNGESFDGRRA
                     AFHLKYKYVKFRSEAPTAEAFITSVASWSRKTGRPYLARIDGEICEVRAVLFRELARL
                     DQMATEKRRETESLPAS"
     gene            complement(4031..5344)
                     /locus_tag="PK590_00290"
     CDS             complement(4031..5344)
                     /locus_tag="PK590_00290"
                     /inference="COORDINATES: protein motif:HMM:NF015194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HOG23068.1"
                     /translation="MRKQAVSFFSTLVNPFAEIHSGERMKTCLMFLYFFLTISTLYIL
                     KPVRSSLFLSEFSAEKLRFINIGEGLFLILIVWLYALLAKRLTHKVLYPLVLGFLIVT
                     MILFWYFIKRDLPYLSAGFYIWQSAFSAMVTTQFWILANDIFRPNEAKRLFGLIISGG
                     SLGGIFGGFLTSFFVRWFPTEDLLLVVALILLACIFLIGIFWKEIIVERPGGMADVAH
                     LPEIKEGNENNKKDVSGKHPFGAGSYIALVVGIVVLAKMSSTIVENQFGGMVQLVIRG
                     KDAITSFYGSFYGWLNVLSFFLQIVVTGKLLKRFGVGISLWLLPLGLAGLSLVSFLVP
                     VLSVSIFYKLYDAGMNYSIQQASKEILYLPIPAKLRRRVKPAIDMLGYRGAKTIAGLY
                     MAVCAPLFGFSVEKFGLLVLLLMPLWFVITWAIHHRLRRYAEEGF"
     gene            complement(5341..5736)
                     /locus_tag="PK590_00295"
     CDS             complement(5341..5736)
                     /locus_tag="PK590_00295"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOG23069.1"
                     /translation="MKLSLNRSAFRYSLGTRGEIAAAEYLRGKKFKILQKNYRCGIGE
                     IDLIAEKGGRIHFVEIKTRSGHGFGTPEEAVDRKKQAKIVKLAEWYLQTMKLRDTRVS
                     LDVLGVTWQENGEPVFRFIEGAFEKEDDR"
     gene            complement(5733..6410)
                     /locus_tag="PK590_00300"
     CDS             complement(5733..6410)
                     /locus_tag="PK590_00300"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOG23070.1"
                     /translation="MSLDPTPTGHSKKEIRRANFAKLLEFDRICFEGPGALAGVDEAG
                     RGPLAGPVVAAAVILGSTDELVGINDSKKLSLVQREKFFEKIVRSANVGIGMADEKTI
                     DTLNIYEATRLAMKRAVQALSHTPAFLLVDGPIRLDVPVAHRGVVNGDQKSASIASAS
                     IVAKVFRDRWMCHLHELYPAYSFDRHKGYGTAVHLEALRTFGPSLVHRRSFAPVERSA
                     RREEAGV"
     gene            complement(6411..6722)
                     /gene="rplS"
                     /locus_tag="PK590_00305"
     CDS             complement(6411..6722)
                     /gene="rplS"
                     /locus_tag="PK590_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOG23071.1"
                     /translation="MSRMKLINQELKTKKMPDFKVGDTLKVHIRVKEGDKTRLQLFEG
                     ICIRKKGRGANASFSVAKESYGDIVEKIFPLYSPIIEKIAVSSKGKARRANLYHLKNK
                     K"
     gene            complement(6780..7514)
                     /gene="trmD"
                     /locus_tag="PK590_00310"
     CDS             complement(6780..7514)
                     /gene="trmD"
                     /locus_tag="PK590_00310"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOG23072.1"
                     /translation="MALLIDIVTLFPDFFKSPLNESLLKKARERGLVNVRVHNLRDWT
                     HDRHKTADDKPFGGGAGMVLKPEPIFECVEEVAKDGWVVLLDPQGDPMSQKLARQLAK
                     KKHLVLIAGHYEGVDQRVKDHLVDQEISVGDFITMGGEAPAVCVIEAVVRLVPEVVGN
                     EESLANESFEHGVLEHPQYTRPREYRGWKVPEVLVSGHHEEVKAWQRKMSTRVTKEKR
                     PDLAVNLKNNSEKVRKTSGHSGRKNK"
     gene            complement(7597..7854)
                     /gene="rpsP"
                     /locus_tag="PK590_00315"
     CDS             complement(7597..7854)
                     /gene="rpsP"
                     /locus_tag="PK590_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOG23073.1"
                     /translation="MSVKIRMKRFGTKDKAKWRVVVCDSKSPRDGRFIEEIGYYDPLP
                     KQEKLVVKEARLEYWVKNGAQVSEALKSLLRRQKKKQKAKA"
     gene            complement(7936..9201)
                     /gene="murA"
                     /locus_tag="PK590_00320"
     CDS             complement(7936..9201)
                     /gene="murA"
                     /locus_tag="PK590_00320"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOG23074.1"
                     /translation="MDKLIIRGRARLKGEVDISGSKNAVLPIMAAALLSEEESVIENV
                     PNLWDVQTMVKLLRSLGEKAILESGVLKIKPGNHLKPFAPYKLVKTMRASVCVLGPLL
                     ARLGEAEVSLPGGCAIGPRPIDLHLKGLEALSAEIQIDHGYVHAKVTSRLRGTDIYLG
                     GGYGSSVLATGNVMMAAVLAQGRTVIENAACEPEVVDLANFLKKMGAKIQGEGSPRIE
                     IEGVKKLRGVSYRVIPDRIEAGTYMFASAITQGDVLIRKMEPGHLNAVIDKLQQAGVR
                     IEKYKNAVRVKRIGKLRASDVTTLAYPAFPTDLQAQAMALMTITPGISIITEKIYPNR
                     FMHVPELGRMGAKIFLEGPSAIVHGVRKLSGAPVMASDLRASAALVLAALVAEGETEV
                     QRIYHLDRGYEDLEKKLTALGATIRREKE"
     gene            complement(9274..10233)
                     /gene="prmC"
                     /locus_tag="PK590_00325"
     CDS             complement(9274..10233)
                     /gene="prmC"
                     /locus_tag="PK590_00325"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOG23075.1"
                     /translation="MVSDPKDGSIRSLLQKGENSLLEAGIEESREETLRILEVLGISR
                     TSVYLGGERKVSEEEERRFLKIVGERMTRRPLAYILGETCFREETLWVNEHCLIPRPE
                     TEILVEKVIAAIREKRQVFCGSPAGENGRMGSGGNPTRKFSFLDIGTGTGAIAISLLR
                     TFLNAEGTLLDISREAIEVARRNVQKYGLEGRARLIPGDFFKLSQGPAESDTPRFVFD
                     VIVSNPPYLSELDLQNIQPELKYEPREALDGGKDGLHFYRRLIPLAKGWLRAGGLLAL
                     EVGAGQSGMVSKWLQDAGYDSIHRFEDYLGIERVVTAWNDKRN"
     gene            complement(10303..11373)
                     /gene="prfA"
                     /locus_tag="PK590_00330"
     CDS             complement(10303..11373)
                     /gene="prfA"
                     /locus_tag="PK590_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOG23076.1"
                     /translation="MRERLEKTEARFVELEATLARPEATRDTYQLQRLSKEIAQIRPI
                     VELFRRYRKLEKELAELRSNLANGQEDPAMRGLIEDEIKTLQSDLERINGQLEDMVLE
                     GCEPDAARDVIVEIRAGTGGEEAALFSANLFRMYSQFAASHGLKVEVMDMSPTGIGGL
                     KEVIFGVSGDRAYSLFKYESGIHRVQRVPKTEASGRIHTSAVTVAVLPEADETEIAIQ
                     PNDLRIDVYRASGAGGQHVNKTESAVRITHLPTGFVVACQDERSQHKNKAKAMRILRA
                     KIYEFQKEKNDAEIAKNRREQVGSGDRSGKIRTYNYPDQRVTDHRIGLTLHDLSNIME
                     GHLDEIVKALAEDEKVHKLTQV"
     gene            complement(11459..11779)
                     /gene="rpmE"
                     /locus_tag="PK590_00335"
     CDS             complement(11459..11779)
                     /gene="rpmE"
                     /locus_tag="PK590_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOG23077.1"
                     /translation="MKKDIHPNYQPCTIDCTCGNSIKTRSVLPRIQVDICSACHPFFT
                     GKQKLMDTAGRIDRFKKKFQDKIVGAKKSTRATPASALKARVADMKKSNFQALKDKIK
                     AEGK"
     gene            complement(11861..13117)
                     /gene="rho"
                     /locus_tag="PK590_00340"
     CDS             complement(11861..13117)
                     /gene="rho"
                     /locus_tag="PK590_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOG23078.1"
                     /translation="MDVASLKKVKISQLQEMAHKLNIENASGLKKQDLIFKVLQAQAQ
                     QSGFMFGEGVLEILEDGFGFLRSPNYNYLPSPDDIYVSPSQIRRFNLRTGDVVSGLIR
                     PPKEGERYFALLKVELINFEETDKAKDKIAFDNLTPLYPNKRIQLETTTEEVSMRVMN
                     LLTPVGFGQRGMIVAQPYSGKTILLQKIANSITTNHPDAYLIVLLIDERPEEVTDMQR
                     SVKGEVIASTFDEPADRHVQVAEIVLEKAKRLVEHKRDVVILLDSITRLARAYNTVAP
                     HSGKILSGGVDSNALHKPKRFFGSARNVEEGGSLTVIATALVDTGSRMDEVIFEEFKG
                     TGNMELQLDRNLFQRRIYPAIDVKKSNTRKEDLLIPADELNRVWILRKVLNELNPTEA
                     MELLVDRLKKTKSNAEFLLKMNNAKD"
     gene            complement(13297..13902)
                     /gene="coaE"
                     /locus_tag="PK590_00345"
     CDS             complement(13297..13902)
                     /gene="coaE"
                     /locus_tag="PK590_00345"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOG23079.1"
                     /translation="MKNKHVIGLTGGFGSGKSTVAKMLRKKGATVIDADQIAHQTLKK
                     SNPVFKKIKALFPEALSRCGRHMDRKKIAEKIFSNPEKRERLEALIHPYVFKQIQKKT
                     EASKKRIVIAEVPLLFETGFDRGCDRTLVVTCRRPVRIARLLAKGFSRGEIASREKAQ
                     MKESLKTKKADFVIDNSGSLAKTQDNVNWLWNRLEKLKSDS"
     gene            complement(13961..16666)
                     /gene="polA"
                     /locus_tag="PK590_00350"
     CDS             complement(13961..16666)
                     /gene="polA"
                     /locus_tag="PK590_00350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOG23080.1"
                     /translation="MTSPRGSRRERLFLVDGNSFCYRAFYAIPGLTNSRGEPTNAIYG
                     FVTMMRKLLEDQRPELLAVCFDPKGKTFRHEQYKDYKAHRKPMPPELVSQIEPIREFC
                     RLSRFSMFEKQGFEADDVIGTLAARAQERGNDVWIVTDDKDAFQLVNDRVKIFQPHKN
                     NLVYDVEGVQKKFAGLGPDKIVEILALMGDASDNIPGVPGIGEKTAIKLMQRFGSVEN
                     ILKNSREISSEKIRNALKENEADLRLSLSLAKIDTQVDLEIDWNDVKVQPADEDALVE
                     FFKRYEFRGFAKRSASQNAKEKEKRDYHTVLDASGLKKLAAALKKSNTFSFDTETTSA
                     DPMKATLVGMSFSMKPYIAHYVPTTSDSYRGPGICLKEALDILGPVLENETIEKFGQN
                     IKYDTMVLRRHGVELKGISFDTMIASYLIDPVKRNHNLDDISLNYLNVKKIETESLVG
                     KGKDQISMADVSLEKISEYACEDADCVMRLVPVLREKIKELGLEDLYKKVELPLADVL
                     GKMEMNGVALDTDMLAELSRSTRREIDGIIQKIYQEAGEEFNLDSPKQLAEILFVKKK
                     LPTMKKTKTGYSTDASVLEKLAVSYEFPKLILEYRERAKLCSTYLDALPGMVNGETGC
                     VHTSYHQTTTDTGRLSSSEPNLQNIPVKTEAGRLVRKAFVPRPRSKGKGKILSADYSQ
                     IELRILAHFCHDPQLVKAFEEDRDIHAYTATLLYGVKEKEVTREMRNVAKTINFSVLY
                     GKTSYGLAQDLQISIQEADGFIRSYFARYARVNDFLESQKEKAKKNGYLTTILGRRSY
                     FPNLQSSNVALRNYAERAAINAPLQGSAADLIKLAMLRIQKRLEDEKSESLMTMQVHD
                     ELVFDAPETEAKALGKRVKEEMENAFELKVPLKVDVTIGDSWYKN"
     gene            complement(16956..18041)
                     /locus_tag="PK590_00355"
     CDS             complement(16956..18041)
                     /locus_tag="PK590_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008101749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOG23081.1"
                     /translation="MAIEEILTEAIKRDASDIHLTVNRPVTFRMVGDLVSVDDNPLTP
                     EMTESFAKEITTEAQRKKIEEVGGVDFGFTFGDKARFRVSCYKQQGHYAMVLRLLPSK
                     FYSFEDIGLPPQLVEILNIPRGLVLVTGPTGSGKTTSLATMLNYINENFPRHIITAED
                     PVEFVHQHKKSVVTQREVGEDVPTFSEAIIKSLRQDPDVVLIGEMRDIATMESAIRAA
                     ETGHLVFSTLHTTGAPRTVDRIIDVFPPEQQAQIRAQLAGTIVAVISQVLVPKLDGTG
                     RVAAFEIMFATPAIRNLIRESKTYQILSEIQTGSRYGMITLDDHLKQLYTQGIISYES
                     ALEVAVDPKELGIQLSKIQPATVKKKK"
     gene            complement(18291..18794)
                     /locus_tag="PK590_00360"
     CDS             complement(18291..18794)
                     /locus_tag="PK590_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23082.1"
                     /translation="MRVPWPWVIAIFGFLLSIRGEQKRGFSLVAIWRETLDLFLTQIL
                     AVTLYHSFLRWSTATSVIEEFGFLILWTLIYLIAKYQKKPDLFLITAVLITHAVVQVC
                     EGWLIQSSLLLVIASGLFIFQAAFKGLRRAILFSGIPPAMKGWPVLCLIAFCISVVMS
                     GMLDILF"
     gene            complement(18791..19408)
                     /locus_tag="PK590_00365"
     CDS             complement(18791..19408)
                     /locus_tag="PK590_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23083.1"
                     /translation="MKMPESFLSRMILACLPFLLYLGEQIDTWRVAGFIVVVYWLMLA
                     AFWFSNRFFPAGAGAQVFLFALALLAQFFWQSAALPPFWILSVFFLFPIDFLRDRKEK
                     RDSKVDLKKFSQYFFERAMTGVGFFIFVVVLEMIMGAVRSPEHTVIEHRPAFAFFLLF
                     LAAFLWKNQPERNASNVSGAPRPMGSRTGEDMTNVGGAGKGKANL"
     gene            complement(19545..20423)
                     /locus_tag="PK590_00370"
     CDS             complement(19545..20423)
                     /locus_tag="PK590_00370"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOG23084.1"
                     /translation="MSAPGVYLESPAPHISSGDSVAKRFWLKSAALLPVLVVVLGTSG
                     AGFLKVLTVSVLGVAGFEWLNAGFFRKKSSLQNGDAILTGVTLAVLLPSHCPALLVLV
                     ATFVAALVIKECFGGLGGRLFQPVVFARVFLDLTFAGGIKSPELFQYAGEPIFLSAIL
                     LSAFLFAIQKKIYFETPLYFVGAMILGAWVTGGQDLLRVAGIAVFAGYFLITDTVTLP
                     LTRRGTNWFAILAACLGVCLGMKYSTVTAICYAILMMNAAVPWIDHWVRPRRYASNTG
                     EMPGSFGARSGLGNKR"
     gene            complement(20420..>21121)
                     /locus_tag="PK590_00375"
     CDS             complement(20420..>21121)
                     /locus_tag="PK590_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOG23085.1"
                     /translation="VGAEKVIFVVEDVERELFELVKSKVYFLKWHWFETRSVKAIYPA
                     GQDHFLPCEIPEDSPVFNASTAFAVYEAVFLQKPFFERIVTVGGECVAEPKNLWLPIG
                     TTFQTAIQLCKGLLREPARLIAGGPMCGMAQASADTVITADTSAILALPKEIAGEKTP
                     EPCIRCNRCVDSCPVFLSPAMITLAVENGEFETAAKWGADECIECGVCSFVCPSHRPM
                     LELIRQVRTERSTSR"
BASE COUNT         5106 a         5877 c         4780 g         5358 t
ORIGIN      
        1 caggtttgtt tacaaattcg gccttttttt cactgcctta agtgggatac accccatctt
       61 gagacaaccg taataaacac ctgctgaaag taaatctccg cattctcgga cttccagcgg
      121 atttcgcctt ccggttccca aatactctca caaagacgtc caggcctcag acgatccttc
      181 agatcatccc gcaaaaaatt aaatccattc ctgtcaaaat ccaggggcct cccgtttatt
      241 ctattgaaaa actggcgctc acgaccgcgg taaccttctt ttcaagagag ctggtgtcgt
      301 cttcaaaaat gcgaaatgct ttcgcgaaaa attgaagggg cagacctctg tttggctccc
      361 ccgcttggtc ccgacggaac atagccgttt cgcaagacaa accaactcct cacggtacct
      421 gcgcccgtct ctcgactcca caggtttcac ccttgattaa aggaaagcac tacagggagg
      481 gttttacagt gggaaaacaa aagtcatttg aatttggagg ttccgctctg atggaacggg
      541 ttcgcgaaca ccttaatctt cgtgtctgat ctgccggaca ccgccctcat ccagaataat
      601 tttgatgtag gggcttggat cctcatcacc ccgtcgactg acacgaattc tgagttcaac
      661 ggtccgattc gggcttatgt agcgcgggtt cagtgaaaac accggttcct gggaggttaa
      721 aatttcgtcg ggtttccgtc tttttgaacc cgcaaaaact tcggccgtat aatcgatcgc
      781 gtccgggtcg ccccacccgt aatggagagc aagatctctg aagcgtaaaa catttccgct
      841 tagttcaaac tggtccagcg gtgtcgtctg tgaaaaccag tagcgggcga tcatgtcccg
      901 acgctcaatc agcgtcttcg cgatatagtc ttcatcttcc gcgtttgaat atcgccccgc
      961 cttgaccatg gcacggacat cttcatccgt aaaactcttc atgatttttg cggcccaaaa
     1021 accgtcagcc tttgtcaaaa cccggaaagt ttcgtaaggt agctggacct tgtgggactc
     1081 aggatcaaaa ccctcgttgc taaaataccc cacggcgggc gactcatacg gcctctcatc
     1141 gacaactcgc cattttttct gccatggctt ttcccacaat ccgagagaaa gaaacgcctt
     1201 ggccgtttcc ccgtaatcga tcatgtattc gtgcccgaac atggggggtt taggaccacc
     1261 atgcgcggct ccgagagcgc tattaaaatc gatcaggtaa tgtttaatga ccggtctccc
     1321 gttctctacc acttccatgt ccagcgtgtt atcctcacgg acatcatagt tgttgagcca
     1381 cattgaaaaa acagatatcg cccgaatttc ccgccggtcg cgatgattga ttgtatcttc
     1441 gggatcatcc ttgcgccggg aataaaaact gaagtacccc gcattcctgc cggggacaat
     1501 cttgctagca gacgccaggt actccccttc ttccgtaatg ggcaaaaagg acagatattg
     1561 atccagcaag tcgggcgtca tttttttccg aaatccggtg tcgtcatagg tgtaggcgtt
     1621 ctcacccgcc acaatgtctt ctggccgaaa ccggaacatc gtgtactggg gcacgttata
     1681 tccgatcgcg taataaaacc gggacgcaat gacttccgcc gcggtcgcca gctccagata
     1741 tccttccgcg tcaaatttca gacaataggc gtctcccctg gcatcccgaa taaaaaaacc
     1801 tggatggagt ccttcctgtt tccccttggt caccgtcatc ggcgcgctca aatcgggccc
     1861 ttcggtttca gaataaccct tctcgagctc ttccgcggag aggcgcttcc gggcgtgccg
     1921 attagtaaaa aacgccgaat ccggaacttc atcaaaaatg tttatattgg cggccggaat
     1981 ttcacggtcg aataacttcc ttccccacct cccgaaatcc aactgattca cgccttcgta
     2041 atagacgttc tggtcaaaca cgtctttgta aagatgggtc gcttttccct taaatttggc
     2101 gcttttcgcg caaagagtcc cgggaagcat gatcgccaat aaggccccaa ccaaaaaaac
     2161 tgtccctttt ttcatcaatc gctcgttcgt catatttctc cagcgcctaa aaaggcaccc
     2221 tgtttttaac atacacccgc gtcccctcat caccatgcgc gacggtaaaa ttcaaaagtg
     2281 tatgtttggc atagctgaga taaaatccga gcccgtagga ttcacgaaaa tcactgaatt
     2341 taaacttgtt aaattcgtta aagacctgtc cttcatccca aaaaaccgag gcgttcagcc
     2401 tgaagtccct gtattcccag atggcgtaac ggtattcgag actgaagaga atcgcgcttt
     2461 ctccataaaa acggttataa acaaaggccc ggtgggctac gctcgtcctt tgagaagacc
     2521 ccatgccgcc caatctcgcc atgtcataaa aagggatcgt gccatgattg atttcatcgt
     2581 tgtgttctcc gaaagcccgg atcgcaagca ctctcctcgg ggacttaaga ctgaaatatt
     2641 ttgccgcgtc aagctgatag gtcaaaaacc tggcctttgt gctatcaact ccctcggtca
     2701 actttacgct cagcttctga taacttccct tggatgcttc gtccttcgta tcccgcgtat
     2761 cacggaccaa ttccatcttc cagttcagct cttctcctcc gtggatcccc ggaatatcct
     2821 gacccgcaaa aacctccgtc atatcaccct tcccgtcgtg cgcgcgatgt ttaatctgat
     2881 tttctttgaa ggcaaaggtc ccgagcacat taagcgacgg cgaaaactca tacccggcgt
     2941 tccacgccag atacacggtt tccatgcgga tcgaggaact atccccgaga ctcgtcctcg
     3001 gaccaattcc ataaaaaggt tcgcggcttc ggtggtcaaa gttgaagttt ccgaacgtat
     3061 aaagaccggt tccggcaata cggttcactc ccaccttcga atccacgata aaaaggttgt
     3121 gcatggcgta tcgcacggag gcattcgcga caacatccgg atgttcgacc tttttccggg
     3181 tcatcgtcat aagatcgagc tccagaccta ccagtgggag aatggcagaa tccgtaaagg
     3241 cgatgtgggg cgtcacgcca tagcccttga ttttgtcgta aatgtggaat gtttttttgt
     3301 cgatatgatg ttttccaata tagacaaggc tttgatcaat aggccacctg acgagacgga
     3361 aaggtatttc cagcaaggca gaaaccggat ccttgaggtt tttggccttg agaggttcat
     3421 gcggatagac gggggcatat atgtcctggg cataagaact tccggcaaaa aacaacaggg
     3481 ccaataccaa ggtgagtagt tttttgtgtt tgtttatcat gacgccggta aactttccgt
     3541 ttctcttctt ttctctgttg ccatttgatc taggcgggcc agctctctga acaaaacagc
     3601 cctgacttcg catatctctc catcgattct cgccagataa ggacgacccg tcttgcgaga
     3661 ccacgaggcg acgcttgtga taaaagcctc ggcagtgggc gcctcggagc ggaacttgac
     3721 atatttgtac tttaaatgaa aagccgcgcg ccggccatca aaactttccc cgttccagat
     3781 aaaatcaaaa tccgattttc caacccgctc tattaaaaaa tcaatccttg cccgctgata
     3841 ctccgaagtc gcgggggtcg tttttaacaa acgtcgaaac gccggagact cttccggaat
     3901 cgccaatttc tccagcatgc aaacatcttg agcttccgtt tcctcttccg gaaaaacagc
     3961 ccgcggttca gccaatatca gcccaagcaa aacaacgctg agcagaacta cggctttatt
     4021 tgatttttct ttaaaaacct tcttcagcat aacgtcggag acggtggtgt atggcccagg
     4081 tgataacaaa ccaaagtggc atcaataata agacaagcag tccgaacttt tcaacagaaa
     4141 aaccaaagag cggcgcgcaa acggccatat aaagtccggc aatcgtcttg gcgccgcgat
     4201 aacccagcat atcgatcgcg ggctttaccc tgcgcctgag ctttgcaggg atcggaagat
     4261 aaagaatttc cttgctggcc tgctggatgg aataattcat cccggcgtca tagagtttgt
     4321 aaaaaatgct cacggaaagt accggcacga gaaagcttac aagggaaagt ccggcaagcc
     4381 ccaggggaag aagccaaagt gagatcccaa ccccgaaacg ctttaaaagt tttcccgtaa
     4441 ccacgatctg gagaaaaaag gagagtacgt ttaaccagcc ataaaaactt ccgtaaaaag
     4501 acgtgatcgc gtccttgcct cggatcacga gttgaaccat tccaccaaac tggttctcca
     4561 cgatcgtaga actcattttc gcgagtacaa cgatcccgac aaccaacgcg atataagacc
     4621 cggccccaaa gggatgcttc cctgaaacgt cctttttgtt attttcattt ccctccttga
     4681 tctctggaag atgcgccaca tcggccatgc cccctggccg ctccacgata atctctttcc
     4741 agaaaatacc gatcagaaaa atacacgcca gaaggataag ggcaacaacc aaaagcagat
     4801 cttcggtcgg aaaccacctg acaaaaaagc tcgtcaaaaa ccctccaaaa attcctccca
     4861 gcgatccgcc gctaatgatc aatccaaaaa gtcttttggc ttcgttcgga cgaaaaatat
     4921 cgttcgccag aatccagaac tgggtagtga ccatcgccga aaaagcggat tgccagatgt
     4981 aaaagcccgc ggaaagatag ggcagatcgc gttttataaa ataccagaaa agaatcatcg
     5041 tcacgatcag gaacccaagt accagcgggt aaaggacttt atgggtcaag cgcttcgcga
     5101 ggagcgcgta aagccataca attaaaatca gaaacagtcc ctcgccaata ttgataaacc
     5161 gcagtttttc ggcgctaaat tcagaaagga aaagagagct ccggacgggt ttcaggatat
     5221 agagcgtgga aatggtcaga aaaaaataaa ggaacatgag acaggttttc atcctctcgc
     5281 cggaatgaat ttccgcgaaa gggttcacga gcgtggaaaa aaatgacacc gcctgttttc
     5341 tcatcgatcg tcttcctttt caaacgcgcc ttcaatgaac cggaaaaccg gctccccgtt
     5401 ttcttgccag gtcactccaa ggacgtccag tgaaactcgc gtatcccgca acttcatggt
     5461 ttgaaggtac cattcggcca atttaacgat cttggcctgt tttttccggt caacagcctc
     5521 ctcaggcgtt ccaaaaccgt gtccgctccg cgttttaatc tcgacaaaat gaattcttcc
     5581 gcctttttcc gcgataaggt caatctcgcc tatcccgcat cgataatttt tttgaaggat
     5641 tttaaacttt ttgccgcgga gatattccgc ggccgcaatc tcgccgcggg ttcccagcga
     5701 ataacgaaac gccgaccggt ttaaagataa cttcatacac cggcctcttc tcttcgcgcc
     5761 gaacgctcga ccggcgcaaa actgcgacga tggaccagag aaggtccgaa tgtccggagg
     5821 gcctccaaat gtaccgcggt gccatatccc ttatgacggt cgaaagagta agcgggataa
     5881 agttcatgaa ggtggcacat ccagcgatca cgaaaaacct tggccacgat agacgccgac
     5941 gctatggaag cggatttttg atccccgttc acgacccccc gatgggctac agggacatcc
     6001 agccggatgg gcccgtccac cagaagaaaa gcgggtgtgt gacttagcgc ctgtacggcc
     6061 cgtttcattg caagccgggt cgcctcgtaa atattgaggg tgtcgattgt cttttcatcc
     6121 gccataccga ttccgacatt ggcggagcga acaatttttt caaaaaactt ttcccgctga
     6181 accagtgaga gttttttcga gtcattaatt ccgaccagct catccgtgga tcccaaaatc
     6241 accgccgcgg caacgaccgg ccctgccaac ggcccccgcc ccgcctcgtc cactcccgcc
     6301 agcgccccgg gtccctcaaa acaaatccgg tcaaactcca gcaacttcgc aaagtttgcc
     6361 cgccggattt ctttcttaga atggcccgta ggcgtagggt cgagggacat ttatttcttg
     6421 tttttgagat gataaaggtt ggcccgtcgc gccttgcctt tagagctgac ggcaattttc
     6481 tcaatgatcg gggaataaag aggaaaaatt ttctcgacta tgtccccgta gctttctttg
     6541 gcaaccgaaa aactcgcgtt ggcacccctg ccttttttcc ggatgcaaat tccctcaaac
     6601 agctgaaggc gtgttttgtc cccttccttc acccggatgt gtaccttgag tgtatcgccc
     6661 actttaaaat cgggcatttt tttcgtttta agttcctgat taatcaactt cattcgactc
     6721 atgactgatc tcctggttaa aaacaggcgt gccaaaaacg ctcctgttca tggttaacgc
     6781 tacttgtttt tcctgccgga atgcccggag gtttttctaa ctttctcgct attatttttt
     6841 aaattcaccg caagatcagg ccttttttct ttcgttaccc gcgtggacat ttttctctgc
     6901 caggccttca cttcctcgtg gtgaccggat accagcacct cgggtacttt ccatcccctg
     6961 tattcgcgcg gccgcgtata ctgcggatgc tccagaactc cgtgctcaaa actttcattc
     7021 gcgagagatt cttcgttccc gaccacttcg ggcaccagcc tcacgaccgc ttcgatcaca
     7081 caaaccgccg gagcctctcc tcccatcgta ataaaatctc ccaccgaaat ttcctggtca
     7141 acgaggtggt ccttcacccg ctggtctacg ccttcataat gtcccgcgat caagacaaga
     7201 tgtttttttt tcgcgagttg acgggccagc ttttggctca tcggatcacc ctgcgggtcc
     7261 agaagaacaa cccaaccgtc cttcgcgact tcttccacgc attcaaaaat cggctcgggc
     7321 ttcaaaacca tccccgcccc gccgccaaac ggcttatcat ccgccgtttt gtgccgatcg
     7381 tgagtccagt ccctcaagtt atgaacccgc acatttacaa gaccgcgttc ccgcgctttt
     7441 ttgaggagcg actcgttcaa cgggctttta aagaaatccg gaaaaagggt gacaatatct
     7501 atcaacaatg ccatagagac gcagggccga gacaagccca gcaatcttaa ccggctaaaa
     7561 agctttgaac cctttagggc tttttagcct ttcaagttaa gcctttgcct tctgtttttt
     7621 cttctgccgg cgcaaaagac ttttcagggc ttcgctcacc tgagcaccat ttttcaccca
     7681 atattcgagg cgggcttcct tcaccaccag cttttcctgc ttgggaagcg gatcgtagta
     7741 accgatttct tcgatgaagc gcccatcgcg cggagattta gagtcacaca cgactacgcg
     7801 ccacttcgcc ttgtctttcg tcccgaaacg tttcattctg attttaactg acaccttgtt
     7861 tcctccttgt tagcgtatag cgtcgagcgt ttcgcgcggg atattttact ccgcacacaa
     7921 caccgttcgc tgaatttatt ccttctctct tcttatcgtg gcgcctaacg ccgtcaattt
     7981 tttttctaaa tcttcatagc cccgatccag atgataaatg cgctgaacct cggtctctcc
     8041 ttccgccaca agggcggcaa ggacaagcgc ggcgctcgcc ctgaggtctg aggccatcac
     8101 gggcgcgccg ctcagttttc tcactccatg cacaatcgcg ctcggtcctt caagaaagat
     8161 cttggctccc atgcgtccga gttcagggac gtgcataaac cggttgggat aaatcttttc
     8221 cgtgataatg ctaatcccgg gcgtaatcgt catcagcgcc atcgcctggg cctgcagatc
     8281 agtcggaaac gccggatacg caagcgttgt aacgtctgag gctctgagtt tcccgattct
     8341 cttgacgcgc accgcgttct tgtacttctc aattcggacg ccggcttgtt gaagcttgtc
     8401 aatcaccgcg ttcaaatgac ccggttccat tttccgaata agcacgtctc cctgggttat
     8461 cgccgaggca aacatgtaag tcccggcttc aatccggtcg ggaataaccc ggtagctaac
     8521 tccacggagc tttttgacgc cttcgatctc aatcctcggc gatccctctc cctgaatttt
     8581 ggcgcccatc tttttcaaaa aattggcaag atcgacgacc tcgggctcgc aagcggcatt
     8641 ttctatcacc gtcctgccct gggcgagaac ggccgccatc atcacattcc ccgtggcaag
     8701 caccgacgat ccgtatcctc ccccaagata aatgtccgtc ccacggagac ggctcgtgac
     8761 cttcgcgtgc acataaccgt gatcgatctg gatttccgcg cttaacgctt ccaggccctt
     8821 gagatgcaga tctattggcc ggggcccgat cgcgcaccca ccggggagag aaacttccgc
     8881 ctctcccaat cgggccaaaa gaggtccgag gacacacacg gaagcgcgca tcgtcttgac
     8941 cagcttataa ggcgcgaacg gcttgaggtg gtttcccggc ttgatcttta aaacccccga
     9001 ttcgagaatg gctttctccc caagcgatct gagaagtttc accatggtct ggacatccca
     9061 aaggttcggg acgttttcga tcacagattc ttcttcggaa agaagcgccg ccgccataat
     9121 cggcagaacc gcgttctttg acccggaaat atctacctcg ccttttaacc tggctctgcc
     9181 ccgaataatt agtttatcca tatcatttcc ggcctatcgc ccaggttttc ctctcttgaa
     9241 catcatcaaa acccaacaaa tactgtttta ttttcagttc cgtttgtcgt tccacgccgt
     9301 aacgactctt tcgatcccca aatagtcctc gaaacggtgg atactatcat aacccgcgtc
     9361 ctgaagccac tttgaaacca ttccggactg tcccgctcca acctccaggg ccaataaccc
     9421 tccggccctt aaccacccct tggcaagagg gataagccgt ctataaaaat gaagcccgtc
     9481 tttcccgcca tcaagcgctt cccgaggctc atacttgagc tcgggctgaa tgttttgcaa
     9541 atcaagttcg gaaaggtacg gcgggtttga aacgatcaca tcaaacacaa agcgaggggt
     9601 atcgctttcc gcgggcccct gggacagctt gaaaaagtca cccggaatca gacgggcgcg
     9661 cccctctaaa ccgtattttt gaacatttcg ccgggccact tcgattgctt ctcgcgagat
     9721 gtcaagcagg gttccttccg cgttcaaaaa agttcgaagt aatgaaatcg ctattgcccc
     9781 ggtaccagtt ccgatatcaa gaaaggagaa ttttcgggta gggttgcctc ccgaacccat
     9841 ccgaccattt tccccggccg gtgacccgca aaaaacctgc cgtttttctc ggatcgccgc
     9901 aataaccttt tccaccaaaa tttcagtttc aggccggggg ataagacaat gttcgttgac
     9961 ccacaatgtc tcttcacgaa aacacgtttc tcccaaaata tacgcgagcg gtcgtcgcgt
    10021 cattctttcc cccacgattt tcaaaaaacg ccgctcttct tcctctgaaa ctttcctctc
    10081 tccgccaaga taaaccgatg tccttgaaat acccagcact tcaaggattc gaagcgtttc
    10141 ttcccgggac tcttcaatcc ccgcttctaa aagactgttt tctccttttt ggagcagtga
    10201 ccgtatcgat ccatctttgg ggtccgagac catcgagaac gcgccttgtg aattactttt
    10261 aaaaaacaat ttcaaaatcc tgattccgga tttcacgcgg gtctatacct gagtgagctt
    10321 gtggactttt tcgtcctcgg ccagcgcctt gacaatctca tcgagatggc cttccatgat
    10381 attcgaaaga tcgtggagag taaggccgat ccggtgatcc gtcactcgct gatccggata
    10441 attataagtt cgtattttcc cggagcgatc tccggaaccc acttgctccc gccggttttt
    10501 tgctatctcg gcatcatttt tttctttctg gaactcataa attttggccc gcaaaattcg
    10561 catggctttg gccttgttct tgtgttggga cctttcatcc tgacacgcca cgacaaaccc
    10621 cgtcggaagg tgcgtgattc gcaccgccga ttctgttttg ttgacgtgtt ggccgccggc
    10681 tccggaagcg cggtacacat ctatcctgag atcattcggc tgaatggcga tttccgtttc
    10741 atcggcctcg ggcaacaccg ccaccgtcac agccgaagta tgaattcgcc cgctcgcttc
    10801 agtcttggga acgcgttgaa cgcggtgaat accgctctca tatttaaaaa gactgtaagc
    10861 gcgatctccg cttaccccaa aaatgacttc cttcaaacct ccgatccccg tggggctcat
    10921 atccatcact tcgaccttca acccgtgcga ggcggcaaat tgcgagtaca tccggaaaag
    10981 gttcgctgaa aacagcgcgg cctcttcccc ccccgtcccc gcgcgaattt ccacgatgac
    11041 gtcacgcgcg gcgtccggtt cgcaaccctc gagcaccata tcctcaagtt gaccgttgat
    11101 ccgctcaaga tcagattgaa gggttttgat ttcgtcctcg atcagtcccc gcatcgcggg
    11161 atcttcctga ccgttcgcga gattgcttcg aagctccgcc agttcctttt cgagctttcg
    11221 gtatcgccgg aacaattcga caatgggccg aatctgcgcg atttccttgc tgagccgttg
    11281 cagctgatag gtatcacgcg tggcctcggg cctcgcgagc gttgcctcaa gctcgacaaa
    11341 cctcgcttct gttttttcaa gccgttcgcg gattctcatc gccaagacac ttcatggaac
    11401 catgacccga tacccgcgcc atcgtgacgg cgcgtggagc ggggatcgtg gtcctgtttt
    11461 attttccttc cgccttaatc ttgtctttca gcgcctgaaa attgcttttt ttcatgtcgg
    11521 ccactcgggc ttttaacgct gaagcgggcg tcgctctggt ggattttttc gcgccaacaa
    11581 ttttatcctg aaatttcttt ttgaagcgat cgatccgccc cgcggtatcc atcagttttt
    11641 gtttcccggt aaaaaacggg tgacaggcgg aacagatgtc aacctggatt cgaggcagga
    11701 cgctccgcgt cttaattgaa ttgccgcaag tgcaatcaat cgtgcagggc tgatagttag
    11761 ggtgaatgtc ttttttcatt ttaatactct cctttttttc gctgacgttc agcttttttt
    11821 gcggatccgg ttccggaatt tttttattcc gaaaacaggg ctagtccttg gcattgttca
    11881 tctttaaaag aaattccgcg tttgacttgg tttttttcaa acggtccacc aggagttcca
    11941 tcgcctccgt gggattcagc tcgttcagaa cctttctcag aatccagacg cgattcagtt
    12001 catccgccgg aatgagcaga tcttctttac gcgtgttcga ctttttaaca tcgatcgcgg
    12061 gataaatccg gcgctggaag aggttgcggt caagctgcaa ctccatatta ccagttcctt
    12121 taaactcctc aaaaataact tcatccattc gcgacccggt gtccaccagc gccgtcgcga
    12181 tcacggtgag actgccgcct tcttccacgt ttctcgcgga accaaaaaaa cgtttcggct
    12241 tgtgcaaggc gtttgaatcc acgccccctg acagaatttt cccggagtgc ggggccaccg
    12301 tgttataggc ccgggcaaga cgcgtaatcg aatccaggag aataaccacg tcccgcttgt
    12361 gctcgacaag gcgttttgcc ttttccagaa caatctccgc cacttgaacg tgtcggtcgg
    12421 cgggttcgtc gaaagtcgag gcaatgacct cgcctttgac ggaacgctgc atgtccgtca
    12481 cctcttcggg acgctcatcg atcaacagca caatcaagta cgcgtccggg tgattggtcg
    12541 tgatcgaatt ggcgatcttc tgcaggagaa ttgttttgcc gctataaggc tgggcgacga
    12601 tcattccgcg ctggccaaat ccgaccggcg tgagaagatt catcacgcgc atcgagactt
    12661 cctcggtcgt ggtttcaagc tgaattcgtt tgttcgggta aagcggggtc aggttgtcaa
    12721 aagcgatctt gtccttcgcc ttgtccgttt cctcgaaatt gatcaactcc accttaagca
    12781 gggcgaaata gcgttcccct tccttgggcg gacgaatgag tccgctcacg acatcacccg
    12841 tccgaaggtt gaaacgacgg atttggctcg ggctgacata aatatcatcc ggtgaaggaa
    12901 gataattata attcggcgac cggagaaaac caaacccgtc ctcgagaatt tcgagtaccc
    12961 cttcgccgaa catgaaaccg gactgctggg cctgggcctg caggactttg aaaatcaaat
    13021 cctgtttctt gagcccggaa gcgttttcaa tattgagctt gtgagccatt tcctgaagtt
    13081 gggaaatctt cacttttttg agactggcta cgtccagcgg cttttccccc gcccgttgtt
    13141 tctgcattgc cttttccaaa gcttcgccgt tcacctgcgc gtcatcgtca cgcatcggag
    13201 ggcgcttgga agggtgaaga tcatcgcttc ggcggtggta gtgatggtgg tttttttctt
    13261 tttgcatcgg gatgttctcc tttgaatgtt taagattcaa ctatcagatt tcagtttttc
    13321 cagccgattc cagagccagt ttacgttgtc ctgggtcttg gctaaagaac ccgagttatc
    13381 gatgacgaag tccgcctttt ttgtttttaa agattctttc atctgcgctt tttctcggga
    13441 cgcgatctcc ccacgggaaa aacctttcgc gagaagacgc gcgatcctga ccggccgccg
    13501 gcatgttacc accagagtcc tgtcacaacc ccggtcaaaa cccgtctcaa acaacaacgg
    13561 aacctcagcg atcacaattc tttttttgga cgcttccgtt tttttttgga tctgcttaaa
    13621 aacatacggg tggatcaggg cttccaatcg ctcccgcttc tcgggatttg aaaaaatttt
    13681 ttccgctatt ttttttcggt ccatatgccg gccacaccgg ctcaaagcct cggggaaaag
    13741 ggcctttatt tttttaaaaa ccgggttgct ttttttaagg gtctggtgcg cgatctgatc
    13801 cgcgtcaatc acagtagcgc ccttttttcg aagcattttc gccacggtgc ttttcccgct
    13861 tccaaaacca cccgttaatc caatgacatg tttgtttttc ataaatctaa aattacacct
    13921 taaaaacacc ggcggaacga agttagtgcc cctcaaccgg ttaattttta taccaagaat
    13981 ccccgattgt cacatccacc ttgaggggga ccttgagctc aaacgcattt tccatttctt
    14041 ctttcactct tttccccaga gcttttgcct cggtttcagg ggcgtcaaat acaagttcat
    14101 cgtggacctg catcgtcatg agggattcgc ttttttcatc ttcaagccgt ttctgaatgc
    14161 gcaacatggc gagcttaatc agatcggcgg cggaaccctg aagcggggcg ttgatcgcgg
    14221 cccgttcggc ataatttctt aacgcgacat tcgatgattg caagttcggg aaataagatc
    14281 gtcggccaag gatcgtcgtg agatagccgt tttttttggc tttttctttc tgactttcaa
    14341 gaaaatcgtt cacccgggcg tagcgggcga aataactccg gataaatcca tcggcttcct
    14401 ggatagaaat ctgaagatcc tgcgccaaac catagctggt ctttccgtaa aggacgctga
    14461 agttaatggt ctttgccacg tttcgcattt cccgggtcac ctctttttct ttgactccat
    14521 agagaagagt ggcggtgtag gcgtgaatat cccgatcttc ctcgaaggcc ttgacgagct
    14581 gaggatcatg acagaaatga gcgagaattc tgagctcaat ttgcgagtag tccgcggaga
    14641 gaattttccc ttttcccttt gaacgcggcc ggggaacaaa agcctttcgc acaagccgtc
    14701 ccgcctcggt cttcaccgga atgttctgga gattaggttc cgagcttgaa agccgccccg
    14761 tatccgtcgt ggtctgatgg taggacgtgt ggacgcaacc ggtttccccg ttcaccattc
    14821 cgggtagcgc gtccaggtac gtggagcaca gtttcgcgcg ttcgcgatac tcaagaatca
    14881 gtttcggaaa ctcgtaggac accgccagct tttcaagcac gcttgcgtcc gtcgagtacc
    14941 ccgtcttggt ttttttcatc gtgggaagct tttttttcac aaaaagaatt tccgccaact
    15001 gtttgggaga atcgagatta aattcctcac cggcttcctg gtagattttc tggatgatcc
    15061 cgtcgatttc cctgcgggtg ctccgggaaa gttccgccaa catatccgtg tcgagcgcca
    15121 ctccgttcat ttccattttc ccgagaacgt cggccagagg aagttctact ttcttgtaaa
    15181 gatcctcaag cccaagctct ttgatttttt cccgaagaac gggcaccagt cgcatcacgc
    15241 agtcggcgtc ttcgcaggcg tattccgata ttttttcaag cgacacatca gccattgaaa
    15301 tttggtcttt ccctttgccg accaggcttt ccgtttcgat tttttttacg tttaaatagt
    15361 tgagagagat gtcatccaga ttgtggttcc gtttcaccgg atcaatcagg tagctggcga
    15421 tcatcgtgtc gaaagaaatt cctttaagtt ccacgccatg ccgacggagc accatcgtgt
    15481 cgtatttaat gttctgccca aacttttcaa tggtctcgtt ttccaatacc ggccccagaa
    15541 tatccagagc ctctttcaga caaattccgg ggccccggta cgaatcactg gtcgtcggaa
    15601 cataatgggc aatataaggt ttcatggaaa aactcatccc aaccagagtc gctttcatgg
    15661 gatccgcgga cgtggtttcc gtatcaaaac tgaacgtatt ggatttttta agagccgcgg
    15721 cgagcttttt caggccggag gcgtccagaa ccgtgtgata gtcgcgcttt tctttttcct
    15781 tcgcattttg tgaagctgac cgcttcgcga accctctaaa ctcataacgc ttaaaaaact
    15841 ccacgagcgc atcttcgtcg gccggctgga ccttgacatc attccagtcg atctcgagat
    15901 ctacctgggt atcaattttt gccaacgaaa gcgagagccg cagatcggcc tcattttcct
    15961 taagagcgtt ccggatcttt tcggaggaaa tctcacgtga attttttaaa atgttttcaa
    16021 cggaaccaaa tctctgcatc agctttatag ccgttttttc tccaatccca ggcactcccg
    16081 gaatattgtc cgacgcatct cccataagcg cgagaatttc tactattttg tccggtccca
    16141 gtccggcgaa ttttttttgg acaccctcca catcataaac aaggttattt ttatgcggct
    16201 ggaaaatttt gaccctgtca ttaacgagct gaaaagcgtc cttgtcgtcg gttacaatcc
    16261 aaacgtcgtt ccctctttcc tgcgcccgcg ccgccaaagt tccgatcacg tcatccgctt
    16321 caaacccttg tttttcaaac atggaaaacc gggaaagccg gcaaaattcc ctgataggtt
    16381 caatctggct gacaagttcc ggcggcatgg gcttgcgatg cgccttgtaa tccttgtact
    16441 gttcatgtcg gaaggtcttt ccttttggat cgaaacagac ggccagaagt tccgggcgct
    16501 ggtcctccag aagtttccgc atcatcgtca caaaaccata aatcgcgttc gtcggctcac
    16561 cccgggaatt ggtcaaaccc ggaatggcat aaaaggccct gtaacaaaag gagtttccgt
    16621 ccacaagaaa cagacgttcc ctccgcgatc cccgagggga tgtcatcgac ataatattag
    16681 gctctggcta ttttctggac atttaggatt taaaacggaa aggcctgaac gatgtggtca
    16741 aagaatttcg tcggaacttt gggatgtgtt cggcagatta aataggctct ttctgaaaaa
    16801 actgactcta ggatattgat tgcgcccctt gagtcaagtt tttatttttc gtttttcaac
    16861 gccgcgcctg aattcagccc gctggcgggg tttgtcacgc gctccaaaaa tcagaaagcc
    16921 tcgcccgtat aaaacatccc ttcccgcgct tttccttatt tttttttctt aacggtcgca
    16981 ggctgaatct tggaaagctg gattccaagc tccttcggat caacggcaac ctccagggcg
    17041 gattcataac ttatgatccc ctgggtgtag agctgtttca agtggtcatc caaagtaatc
    17101 atgccgtacc ggctccccgt ctggatttcc gacaaaattt gataagtttt agattcccgg
    17161 atcagattcc ggatcgcggg cgtggcaaac ataatttcga aagccgcgac gcgtccggtt
    17221 ccgtcaagtt tcggaacgag aacctgcgaa atgacggcga caatcgttcc cgcgagctgg
    17281 gcacgtatct gggcttgctg ttccggggga aacacatcga tgatacggtc aaccgtgcga
    17341 ggcgccccgg tcgtatggag cgtggaaaac acaaggtgtc ccgtctccgc cgcgcggatc
    17401 gcggattcca ttgtcgcgat atcgcgcatt tccccgatca ggaccacgtc cggatcctgt
    17461 cgtagcgact tgataatagc ctcagaaaaa gtcggtacgt cctccccgac ttcgcgctgg
    17521 gtcaccacgg attttttatg ctgatgaacg aattccacgg gatcttccgc cgtaataatg
    17581 tgtctcggaa aattttcatt gatgtaatta agcatggtcg cgagcgatgt cgttttcccc
    17641 gatccggttg ggccggtcac gaggaccagt ccgcgtggaa tattcaaaat ctcaaccagt
    17701 tgcggaggca ggccaatgtc ttcgaacgag taaaacttgg agggtagaag gcggagcacc
    17761 atggcgtagt gtccctgttg cttataacag gaaacgcgaa aacgagcttt atctccgaag
    17821 gtaaaaccaa aatcaactcc ccccacctct tcgatttttt ttcgctgggc ctctgtcgta
    17881 atctctttgg caaacgattc ggtcatttcg ggagtaagcg gattatcatc caccgagaca
    17941 aggtctccta ccatgcgaaa ggtgacgggg cggtttactg taagatggat gtcggatgcg
    18001 tctcttttaa ttgcctccgt taaaatttct tcgatcgcca taaaaccctc tttctttgtt
    18061 ttctgaattt attttgactt tgctttcatg tttcgtcttt aaacaaaaaa tctgaacagg
    18121 cgcagggttt ttacgatccg gcgcgacatc ttgatcctcc cgcccctcgc tcgtttttct
    18181 cagccatttt tttaattggc ccgatgaagc acgagaaggt tcgcgaaaat taaacattcc
    18241 acccgagacg agacatcgcg tcaaattcat ggtggatcgc gtccatccgc tcagaaaaga
    18301 atatcaagca ttccactcat cacaaccgat atacaaaacg cgatcagaca aagcactggc
    18361 catcccttca tcgccggcgg aattccggaa aagagaatcg cgcgccttaa tcccttgaac
    18421 gccgcctgaa aaataaaaag tcccgacgcg attacgagta gcagcgagct ttggatcaac
    18481 cagccttcac aaacctgcac gacggcatgc gtgatcaaca cggctgtgat taaaaaaaga
    18541 tccggctttt tttgatattt tgcgatcagg tagatgagcg tccagagaat caaaaatcca
    18601 aattcttcaa tgacggaagt tgcagtactc catcgcaaga aggaatgata aagggtcacg
    18661 gccaaaatct gcgtaaggaa aagatccaat gtttcacgcc aaatcgcaac cagtgaaaac
    18721 cctcttttct gttctccacg tatggacagt aaaaacccaa atattgcaat gacccacggc
    18781 cagggtaccc tcataaattc gccttccctt tcccggctcc gccgacattt gtcatgtcct
    18841 cgcccgtccg cgatcccatt ggccgcggcg ccccgctcac gtttgacgcg tttcgctccg
    18901 gctggttttt ccagagaaat gcggccagaa ataacagaaa aaaagcaaaa gccgggcgat
    18961 gttcaataac ggtatgctcc gggcttctta ccgctcccat gatcatctca agcacgacga
    19021 cgaaaataaa aaaacccacg ccggtcatgg ccctctcgaa aaaatactgg gagaactttt
    19081 taagatccac tttcgagtct cgcttctctt ttctgtcccg aagaaaatca atgggaaaca
    19141 gaaaaaaaac actgagaatc caaaaaggcg gcaacgccgc cgactgccaa aaaaactgcg
    19201 ccaaaagggc cagagcgaac aaaaaaacct gggctcccgc gccggccgga aaaaaacggt
    19261 tcgaaaacca gaaggccgcg agcatcagcc agtaaaccac gacgataaag cctgccactc
    19321 gccaggtatc aatttgctct cccaaataaa gcagaaacgg cagacacgcc aaaatcattc
    19381 ttgagagaaa gctttcgggc atcttcatcg gacgaaaacc tgtttcattt gagcggactt
    19441 ttccgggcaa cttgtcattt cccggatctg aaaaaacaac gctaccatac cgaaaagtac
    19501 ggctatcaga aaaaaccatt gaaatatttt agcggctttt tttctcatct tttgtttccc
    19561 agtcccgacc gcgctccaaa cgaccccggc atctcacctg tgtttgacgc gtatcgcctg
    19621 ggtctcaccc agtggtcaat ccagggaacc gccgcgttca tcatgaggat cgcataacaa
    19681 attgccgtga cagtggagta tttcatgccg agacacaccc ccaagcacgc cgccaggatc
    19741 gcaaaccagt ttgtcccccg gcgtgtcaac gggagcgtaa cagtatccgt gattaaaaaa
    19801 taaccggcaa aaacggcgat tccggccacc cttaaaagat cctgcccacc cgtaacccac
    19861 gcgcccagaa tcatcgctcc gacaaaataa aggggcgttt cgaaataaat ctttttttga
    19921 atagcaaaaa gaaacgcgct caggagaatg gctgaaagga agatcggctc tccggcgtat
    19981 tgaaaaagct ccggagattt tattcccccg gcaaatgtga gatccagaaa aactcgcgcg
    20041 aacacgacag gttgaaacag tcgccccccc aaaccgccaa aacattcctt gatcacaagg
    20101 gccgcgacaa aggtcgccac cagaaccagt agcgccggac aatgcgacgg aagaaggacg
    20161 gcgagcgtca cgccggtcaa aatcgcgtca ccattctgaa gggagctttt cttcctgaaa
    20221 aatccggcat tcagccactc aaatcccgcc acacccaaca cggaaacggt cagcaccttc
    20281 agaaaacccg ccccgctcgt tcccagtacc accacaagca ccggtaaaag ggcggcgctc
    20341 ttaagccaga accttttggc aactgagtct ccagaggaga tgtggggggc tggggattca
    20401 agataaactc ccggcgcact catcgcgagg tgctcctttc cgttctgact tgacgaatca
    20461 actcaagcat cggcctgtga gaaggacaga caaaactgca gacaccacac tcaatacatt
    20521 catccgcccc ccattttgcc gctgtttcaa attccccatt ttcgacggcg agagtaatca
    20581 tggccggaga caaaaatacg gggcaggaat ccacgcaccg gttacatcgt atgcacggct
    20641 cgggagtttt ttcccccgcg atttctttcg gtagcgccaa aatcgcggaa gtatccgcgg
    20701 tgattacggt atccgcgcta gcctgcgcca taccacacat ggggcccccc gcaattaacc
    20761 gggcgggttc acgaagtaat cccttacaaa gttgaatggc cgtctggaag gttgtcccga
    20821 tgggaagcca gaggttcttg ggctcggcaa cgcattctcc accaacagtg acaatgcgtt
    20881 caaaaaacgg tttttgaaga aagacggcct cataaaccgc gaacgccgtg gacgcgttaa
    20941 acacgggact atcttccggt atctcgcatg gcagaaaatg gtcctgcccc gcgggataaa
    21001 tcgctttcac gcttcgcgtt tcaaaccagt gccattttaa aaaatagact ttgctcttaa
    21061 ccagttcaaa aagttcgcgt tcaacatcct cgacgacaaa aatcaccttt tcggcgccga
    21121 c
//
LOCUS       DAOSIO010000004        20606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_2836_length_20606_cov_4.816067, whole genome shotgun sequence.
ACCESSION   DAOSIO010000004 DAOSIO010000000
VERSION     DAOSIO010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 20606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20606
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2836_length_20606_cov_4.816067"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2580)
                     /gene="metH"
                     /locus_tag="PK590_00380"
     CDS             complement(<1..2580)
                     /gene="metH"
                     /locus_tag="PK590_00380"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOG23086.1"
                     /translation="MPNLKCRELCYNTRLMTHPFLELCKKRVMVLDGAMGTNLQKQFL
                     TPQDFGGKDGCNEYLVITKPESVRKVHEDFLAVGCDAIETNSFGSNRIVLSDYGLEAR
                     AYELNEKAAQIAREAARKYSTPEHPRFVLGSIGPGTKLPSLGHITFDDLKDATREQIK
                     GLMAGGVDGLIIETCQDLLQIKAAVIAAEECFAEAGRRLPVITQVTVESAGTMLLGSE
                     LAAAITTLEMFPVDVIGMNCATGPAEMVEHVRTLCESSRKLISVLPNAGLPENIDGMA
                     CYRLTPKEFADYQERFVKEFGVSIVGGCCGTTPEHLAAVVARVKGIQPRERHPVHEPA
                     CASLYNRVAYHQVPAPLLVGEQTNANGSRRFKQILERDDYDGLLEIGREAVREGAHVV
                     DLCVAFTGRDEKKDMAEAVSRFNQHVTAPLMIDSTEPQVLEEALKRISGKPIINSINL
                     EDGEDRLATICLLAKKYGAAVVALVIDEKGMARSRGEKLAVAKRIFDLAVKKHGIFPE
                     DIFFDALTFTIGSGDENSRNAAVETLEAIKLIKKELPGARTILGVSNVSFGLSPQARK
                     ILNSVFLHEALSAGLDAAIVHAERIRPIAKLEASDIEVSKNLIYNRWVDERDPLEVFI
                     KHFETRLSPEEQKAREPSGESKLSKGPVEDRLKAFVIDGMKDGLEAALDEALKKYRPL
                     EIINQILLEGMKVVGENFGAGKMQLPFVLRSAEIMKRAVSYLERFMEKKEGSERGKIV
                     LATVKGDVHDIGKNLVDIILSNNGYKVINLGIKQPIDAILKAAEEHKADAIGLSGLLV
                     KSTHIMKENLEEMNYRNITTPVILGGAALTERYVCEDLARLYRGSVFYGKDAFTALKI
                     MEKV"
     gene            complement(2601..4046)
                     /locus_tag="PK590_00385"
     CDS             complement(2601..4046)
                     /locus_tag="PK590_00385"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOG23087.1"
                     /translation="MMPKKISAEPLREMVEKIPSTPGVYLMKDRSGEILYVGKAKSLH
                     KRVRSYLSVSPALPKIKVMMSKVCLIDHIETPSEVDALLLEAQLIKKYRPRYNKELKD
                     DKSYPLLKITGDEFPRLTITRQKGDRKALYYGPYTDSKLLRQAVALIHNLFPIRKCQA
                     LPKRACLYYHIRQCLAPCVHPEVKRDYDRLIREVRHFLGGGKRSFLEYLTDRMKRASR
                     ELRFEDAQFFKEQIVALGQLKRKRFFPGRKEEGAGLSATMDLKRALKMAVCPAKIVCF
                     DVSNIQGDESVASKVSFFRELPDKIHYRRYKIRDVTGINDYAMIREALGRMLRGIREG
                     RESFIPDLIMIDGGRGHLNTALKVLREEGMERIEMIAIAERFEHVFSPKFREPIIFPA
                     GTPVLHLLEKVRDEAHRFAITYHRSLKSKKLESSLLDQIDGVGARRKRILLKSFDTLE
                     DLKRANLERLRGIQGIDSVTAQKIFDFFHPR"
     gene            complement(4129..5772)
                     /gene="murJ"
                     /locus_tag="PK590_00390"
     CDS             complement(4129..5772)
                     /gene="murJ"
                     /locus_tag="PK590_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOG23088.1"
                     /translation="MSSSVEKSGDNVTRGPRPVNEVPKTGFGKKSLVRAAVLVSAMTL
                     LGRVLGLIRDILSAKTFGTRWQWDAFIYAFMIPNLFRRIVGEGGLTSAFIPVYNETKT
                     KQGVAEAFRFANVTLCFLACALLVFVIAVQGLITAILAAGHYSEMLTLTLNLLRILFP
                     YLWFLSLYALGMGILNSHGHFLAPSLGSVILDGVWIFAILWVPSWVGTDYADRIRWLT
                     YTLLFAGFLHVAVEIPPLWKIGFRFKFIWDLAHEGLKKTWKLLLPVVMSFAVVQINIT
                     VDATLGLVIGPGANSSLWYGNRLMQFPLGIFALAMGTALLPMLSQQIASGEREAAKRS
                     LSFALRSVFFIIIPSSVGLIVFREEIIRFLFERGEFDPVSTARSAAVLLGYTIGLFAY
                     SGQKIMAAGYYAAHDTKTPMRTALVSLFLNIVLNLILMIPLKEAGLALATSISGIVQF
                     GQLIYYYPKKVGEFPFREIARSFFRILLASAAMAGICYASLAGLRSVVPGTDTLAQGI
                     QVLGSILIAIISYLALCFLFRVPEIHEAVEWIQKKRKRK"
     gene            5777..6043
                     /gene="rpsT"
                     /locus_tag="PK590_00395"
     CDS             5777..6043
                     /gene="rpsT"
                     /locus_tag="PK590_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOG23089.1"
                     /translation="MPNIDSAAKRIRSDARKRARNQAVLSEIKTLSKKLVQLTASADK
                     AAAVAQELISRYDRAVTKGVIPRGRADRRKSRVTKFVTKLSAKK"
     gene            complement(6052..7005)
                     /gene="holA"
                     /locus_tag="PK590_00400"
     CDS             complement(6052..7005)
                     /gene="holA"
                     /locus_tag="PK590_00400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOG23090.1"
                     /translation="MTSRFFLIIGDPFLRSQKIKSIVSDLEKKAKGSFDHQTYRLADT
                     PLDSILSNARTLPFLASGQIFYIQVAEKLTQGEMEILKRYAMSPDEKTCLIFEIDSSE
                     GHSELINLAKSRGKVVRLAEEEARSLAQNYLQQKLARFKKTITPGARQRILEMCGDAI
                     VFLDTMLDRILQYSGEAQLIDENMVCLFEENWRGVNVFQLADAILERDRGKARQTFHE
                     LLENSESEPRFLIGIIHWQLRQLWQGAVLREAGTSEAEILEKVKVPLFKKGPFMSAIN
                     RYGVARLEAALEALHQLDRKTKTGLAHPVSGTETWIMEFVS"
     gene            complement(7074..7607)
                     /gene="lptE"
                     /locus_tag="PK590_00405"
     CDS             complement(7074..7607)
                     /gene="lptE"
                     /locus_tag="PK590_00405"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HOG23091.1"
                     /translation="MTFAKRFIWIFLAGVIIVPLFGGCGYTTKTVLPRDIKTIYVETV
                     KNKIPILEMRSYQQGLEMDITNAVIARLQIDGNLRVAPEGQADSILQMDLKIYEQEGL
                     RFTQLESVEEYRMFIVMHLRLVDAKTNEIIWEEPDFSGAREYYVTDIKQIGEQAAVEQ
                     AVDKLAKNIVDRIVEDW"
     gene            complement(7618..10602)
                     /gene="bamD"
                     /locus_tag="PK590_00410"
     CDS             complement(7618..10602)
                     /gene="bamD"
                     /locus_tag="PK590_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOG23092.1"
                     /translation="MKFSSIVALIIPVSLVLVPGPRASADWVWSPERGEFVSTDEGGQ
                     DDSQDAFDQALDYFQEKKLDSAAEGFELILKKYPKSIVAPEAQYRLGTIHEEKGDLVK
                     AHKAYQNLLKTYPQSERFEEVVEREYEIGSAFLSGEKGVLLGLKIRPSIPMAIDIFKG
                     IVEAAPFSAFGDKAQFQLGNAYMKSGQYDPAMEAFQEVIDQYPQSPLVQDARLQMAEA
                     SYAKSQVEIRDQSALDDVSQQVERYLQKYGSSAEADRATQIRREVDERNAEKNYRIGA
                     YYEKENYLESALIYYRDTVKRYPDTAWGQKAADKLKSLEQPVTYLTAKSDEIKTEIAR
                     LEGELAAIGKSDPQKQKDLEREIKALKKKLDAVDGSKSDALLRRKQDLKRRERELKEK
                     FKAFELKKKRYQNNTSPDFQRAMDRWLASLEAERDLILEEKARLADWRAELGVSSEPF
                     YQNLLSFMQPQSPVEKVRETGEKDLYKISKAKKDLFLEKEKLYKRYGELQADLAPQLT
                     ETGVEIKRLRGSERQRISEKPSDQLSDRERSIKALDEKLDGKLAEYEKIFGKLAEQEL
                     EAVLARQAGVQTGGPVHFDVGGDVQKKSMDELLALKMHLAEKVRAQQNVVDTLSHAFN
                     RELALQEQREILKSLDARDDADLKKLRKQIKSVEKDIRQRYQDIQDRHEKKKQLLDEL
                     DQVLNGDRHTTFRKTKNAVTGVAYLARSFVFGLPNRDEQLNRQAKRSSQQKVKELQQA
                     VELESLVIEAQSIELARLEKQHEILTAKASLAGGYKFRSSLVKVPYEYIDEAVHNARR
                     ILPSGERRDTLLNRLDSQSRELEAAKKQIERVDSEIRSRSSTVAATAAEPDQASVEDR
                     EKMLREEIQRLFEQLEVQYSIYAEEQGILSADYRDKLEKEMDAPGAEKERVKRYKEFR
                     GIENSLVKLIGEELKIESEEKEVLQKRIQEADKILPGVTSKAMTQEILDEKKRAEQRI
                     SDLDTRHDFLMKEQQRYQKKDL"
     gene            complement(10694..12937)
                     /locus_tag="PK590_00415"
     CDS             complement(10694..12937)
                     /locus_tag="PK590_00415"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOG23093.1"
                     /translation="MKFPFLPLAISLSLGMVSSRYAPSVTPVVVSLLVILLPFAWFLR
                     GRKSFFPVFLILIFTSGWIGFSIDGLKPENSAHQFVSDRRLAVTGIVATLPDVKCRGR
                     GRNISFVLKSTEVNLGSRRYPKPRAVRGQVWVYWIQPATVPSVGDKVRLYGLLKKPRQ
                     AQNPGGFDQTRYLAEKNIHAMIEVIGPNSGRIVAEGSVFQPARWIGKLRLTIASKIDA
                     LYQRPESSMLKAVILGFRSEIASDLKSAFVKTGTIHLLSISGIHITMVAGGFYLLFLA
                     LGAGRRMTAMLTMAVVLCYVSISGMGLPAQRAGWMAGIVLLGVIWGRPSLSMNALCVA
                     FFLMILARPRDLWNVGFQLSYLCVFSLIAFLPRFSSCRSLPGSLGGSLVVTAGTFPLV
                     LYYFNVFSPVSLMANFVAIPVFDAALFLSLFSLLFSAVPLLGEWLIAISSAFIQLMSA
                     WIVQLSRLTWGYWFLSKPTPWQLFGYYGSLAGLLFFWGRNSLWKKQMAILCAGICLVT
                     GLTMMRTEPDAGFFVNVLCAGRDPVIHVRFSGGRNWLIGAGRRFPSDQGEKIILPYFQ
                     QKGIRALDGILVPGSYKKHWGGLMTILRELRVRTVIFPQDFHVREELSRYSDKFRYVR
                     SGETILELPDRESLMMEWAGKKDNAVFIEAGQQKICFAPCGLDPLRISRISPPPDILI
                     LPFVKKIAPWNGVEEVRLWVLPGAETEAALFLQKQAGQRYFLNEKGALNIRVFETGFL
                     TKNLVKV"
     gene            complement(13132..13740)
                     /locus_tag="PK590_00420"
     CDS             complement(13132..13740)
                     /locus_tag="PK590_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23094.1"
                     /translation="MSLIQAALNKAASSQKQKMETPSPETAGKIKTSSKPLPDWPKVM
                     DERLERELREVQRHYGSRRALGWKILAGVFILLSLVVLGTILQRVSNTGSPELTGGGV
                     PKKLNSAVPEGVRDPDGNTRAGFSSLAKIVPVMSNKNLSHQSRHALKLTGILDLGEGA
                     RAVIDDRILAVGDLVGNEWVVMKIDPDEVTLESRGEVLRLKL"
     gene            complement(13737..14546)
                     /locus_tag="PK590_00425"
     CDS             complement(13737..14546)
                     /locus_tag="PK590_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG23095.1"
                     /translation="MYESFFHLTEPPFNVTPDPRFVYLSRHHQSALSSMKYGVDRRVG
                     FIQITGEIGAGKTTLSRMFLQQIGENVHTSLVFNPKLSEIELLEVIVEDFGLKPASKK
                     RKACFDALNRFLLEESSKGFNAVLIIDEAQLLPPKALEQIRLLSNLETASQKLLQIIL
                     MGQPELNDLLKRRDLRQLKQRIAVRCHLPELSLEEVGLYIRHRLKVAGATDDIFSLEA
                     IGRIHELSGGIPRVINILADRALLAAFAAGAQRIDAPMVECAEADLQGIAV"
     gene            complement(14613..16658)
                     /locus_tag="PK590_00430"
     CDS             complement(14613..16658)
                     /locus_tag="PK590_00430"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOG23096.1"
                     /translation="MPKVEIKKKKLLVGGKEVPLISGEVHYWRLNPNCWKHTLSRVKE
                     MGLKIISTYVPWDFHEYQRGKFDFAGKTDPTRNLKAFLDLTRKEGFWLVIRPGPYIYS
                     EWPHDGVPAYAYKYHRLHPRFLDYASVYLKEVTKVFKPYLATRRGGHIILLQADNEID
                     PWPDVFGQQYGLSGPPGLFQDFIARLYENDLEALNNAWGTRYASFKEASPFIATMLAN
                     AQGIPLKGDKELKRNLDYFKFKYDYATKVAKWNVETYRNLGIDVPIYLNTYPFFYAHD
                     WSRLQSVSDMVGVDLYPSNELAEDEFEHRKFCDKIRYLSSVSHLPFIAEFQSGVWHGR
                     HYETSVLTPNHYRMICLSAFLGGIKGWNWYMLVNRDNWYMSPINEWGRTRGELYAVFK
                     QIVRAFHSLRPTSLEKLTEVAVTFNPLQYAARTLSHDNPILQALYHGDVDYDVCDPRQ
                     AVPDKKVVFYSGNQWLDRISHENLRRYVERGGALVSFVSYPRKDENFNPCQVTGFEDP
                     ERILFEFKRQFKIRLDKGQPEIQVTSSVYVFKADGCQKIQAEIKGYGNLTVGYIKKIG
                     KGRVIHLGVDPTPELVLGVLRFLSVPLFAYSVTRDVKTAIFRRGAKHYLVMVNNGNED
                     KSGTVFLPALEKCRGDMIVRDLITGEKLDQTGERRRVFNGPINRKDGRVFEIAFRKA"
     gene            complement(16810..19287)
                     /gene="leuS"
                     /locus_tag="PK590_00435"
     CDS             complement(16810..19287)
                     /gene="leuS"
                     /locus_tag="PK590_00435"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOG23097.1"
                     /translation="MVSGFKAYPFDEIEKKWQAYWLTHKTFRSPDPDECSPQKPKYYV
                     LDMFPYPSGSGLHVGHPEGYTATDIIARYKRMRGFNVLHPMGWDAFGLPAEQYAIETG
                     THPSITTKKNVETFRRQIQMLGFSYDWDREVDTTDPDYFKWTQWIFLQLYKKGLAYEA
                     EIPVWWCEGCKAVLANEEVRSDGTCDRKGHKDVFRRPLRQWVLKITAYAERLLEDLKY
                     VDWSEAIKEMQRNWIGRSEGAEVQFKIADSNLEVKVFTTRPDTLFGATYMVLAPEHPL
                     VEKITSPQCRAQVEKYRQESSRKSELERAQLEKDKTGAFTGAYAINPVNDSKIPVWIA
                     DYVMMGYGTGAIMAVPAHDTRDFEFATRFNLPVIQVVQPDDPETEWRGFVGEGTSVNS
                     TGPDVSITGLPTAEAKKKITDWLAAKGLGKASVQYKLRDWLFSRQRYWGEPIPLIHCA
                     QCGIVPVPENELPLRLPEMTDYTPTGSAEPPLAKVRDWVQCKCPKCGAVANRETNTMP
                     QWAGSCWYYLRYIDPKNPDAVVDKKKEQYWMPVDLYVGGAEHAVLHLLYSRFWHKVLF
                     DLGFVSTPEPFRKLVNQGMILGEDNQKMSKSRGNVINPDDVVRQYGADSFRLYEMFMG
                     PLEMTKPWATSGVEGVYRFLGKVWRLFMDKDGNLHPNIQSRASSQTELRALHQAIRKV
                     TEDLESLRFNTSISALMIFVNEISAHPDHSREVLEKFLLLLSVFAPHLSEELWQKMGH
                     SGSLAYEPWPEYDDQYLIQSEYEMAILVAGKVRAKMMVSAELGQEEIRKKVLEQPRVR
                     EIVGEKKIIKMIVVPGRRTVNLVLGPG"
     gene            complement(19392..19477)
                     /locus_tag="PK590_00440"
     tRNA            complement(19392..19477)
                     /locus_tag="PK590_00440"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19441..19443),aa:Leu,seq:caa)
     gene            complement(19673..>20606)
                     /locus_tag="PK590_00445"
     CDS             complement(19673..>20606)
                     /locus_tag="PK590_00445"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOG23098.1"
                     /translation="RKGVNPGPFCYRYRLEDQDLEKSIRRCGILNPLVARREKGAIRM
                     IAGHKRLYVACRLGMKRVPVIFIEKKISDPDAFQISLVSNWKQEISDMDRVVAIRKAI
                     RDFRFRPERVRDTLLPLLGLPAEESFLEYYRKADALEDEIKNLLDRRQIPFKSCALLL
                     RFSPADQKAFARFLDRRAHLTSSQCLQSLEWLGDIVKRDETTLRDILRLKSFRAISEA
                     KSLDPRQRGDLFLAEVRQRRFPGLTAFLKDFSTRSRKITFRAKNLRIEPVASFEEPGI
                     ELHARLRNAADVDGILKLLSGSVQELNALLSPVF"
BASE COUNT         5070 a         5787 c         4854 g         4895 t
ORIGIN      
        1 gaccttttcc atgatcttga gggccgtgaa cgcatccttc ccataaaaaa cgcttccccg
       61 gtacaggcgg gccagatcct cgcagacgta ccgctccgtc agcgccgctc ctcccaaaat
      121 cacgggtgtt gtaatgttcc tgtaattcat ttcctccagg ttttctttca tgatgtgcgt
      181 ggattttacg agcaaacccg aaagtccgat agcgtccgcc ttgtgttcct cagccgcctt
      241 cagaatagcg tcgatcggtt gcttaattcc aagattgata accttgtatc cgttattgga
      301 aaggatgatg tcgacgaggt ttttcccgat gtcatgaaca tcccccttga ccgtcgcgag
      361 cacaatcttc cccctctcgg aaccctcttt tttttccatg aaccgctcga gataggatac
      421 cgcccgcttc ataatttccg cggaacgaag cacaaaagga agttgcattt ttccggcgcc
      481 aaaattctct ccgactactt tcatcccttc caaaagaatt tggttgataa tttcgagtgg
      541 cctgtatttt ttcagggcct cgtccaaagc cgcttcaagc ccgtctttca tcccatcaat
      601 cacaaacgcc ttcagcctgt cttcaaccgg ccctttcgac agtttcgatt ccccggatgg
      661 ttcccgcgcc ttctgctctt cgggactgag ccgggtttca aaatgcttta tgaaaacttc
      721 gagaggatcc cgctcatcca cccatcggtt atagataaga ttttttgaaa cctcgatatc
      781 tgaagcttcg agctttgcaa tgggccgtat tctttccgcg tgaacgatcg cggcatccag
      841 tcccgccgac aaggcctcgt ggagaaacac gctgttgagg atttttcgcg cctggggcga
      901 aagcccaaac gacacgttac tcacgccaag aattgtgcgc gccccgggca gttctttttt
      961 aataagcttg atggcttcca gagtttcaac cgccgcattt cgggagtttt catccccgct
     1021 tccgatcgtg aaagtcagcg catcaaaaaa aatatcttcc gggaaaatcc cgtgtttttt
     1081 gacggcaagg tcaaaaatac gctttgccac cgcaagtttc tcgccgcgac tgcgggccat
     1141 tcctttctca tcgatcacaa gcgcaaccac ggcggctccg tattttttcg cgagtaaaca
     1201 gatcgtcgcc agacggtctt ctccatcctc cagattgatt gagttgataa tgggctttcc
     1261 ggaaatccgt ttaagggcct cttccagcac ctgaggttcc gtcgagtcaa tcataagggg
     1321 cgcggtcacg tgctggttaa aacgcgacac agcttccgcc atatctttct tttcatcccg
     1381 tccggtaaag gcaacacaaa ggtcgacgac atgggcgcct tccctgacgg cctcccggcc
     1441 gatttcaagc aacccgtcat aatcgtcacg ctcaagaatt tgtttaaacc gacgactgcc
     1501 attcgcgttt gtctgttctc caacaagaag cggcgccgga acctggtgat aggcaactcg
     1561 gttgtataag gacgcgcagg caggttcatg gacaggatga cgctccctgg gctgaatccc
     1621 cttaacccgc gccacaacgg cggccagatg ctcgggggtt gtcccacagc atcccccaac
     1681 gatcgacacg ccaaactcct tcacaaaccg ctcctgataa tcggcgaatt ctttcggggt
     1741 taaacgataa caagccatcc cgtcaatatt ttccggtagg ccggcgttcg gaagcactga
     1801 aatcagtttc cttgaacttt cgcagagggt tcggacgtgc tcaaccattt ccgccggacc
     1861 cgtggcgcaa ttcatcccga tcacgtccac gggaaacatt tcaagcgtgg tgatagccgc
     1921 tgcaagctcg gagcccaaca acatggttcc cgccgattca accgtaacct gggtaatgac
     1981 ggggaggcga cggccggctt cggcaaaaca ttcctcagcc gcgatcaccg cggccttgat
     2041 ttggagcaaa tcctggcagg tctcgataat cagtccgtca actcctccgg ccatcagccc
     2101 cttgatctgc tctcgggtcg cgtccttcag atcatcaaaa gtgatgtgcc ccagggatgg
     2161 gagcttggtc ccgggcccga tggacccgag gacaaaccgg ggatgctccg gagtagaata
     2221 ttttctcgcg gcttcccggg caatctgagc ggctttttcg ttaagctcat aagcacgggc
     2281 ctcaagcccg taatcggaaa gaacaatccg atttgagcca aagctgttcg tctcgatggc
     2341 atcacaaccg accgcgagaa agtcttcgtg aaccttacgg acggattcgg gcttcgtaat
     2401 gacaaggtac tcgttgcaac cgtccttgcc gccaaaatcc tgtggcgtga gaaactgttt
     2461 ttggagattg gtgcccatgg cgccatcaag caccatgacc ctttttttac aaagttcaag
     2521 aaacggatga gtcataaggc gggtattgta gcacaactcc cgacatttga gatttggcat
     2581 ttgaaattcc gcgcgggaat ttagcgagga tggaagaaat cgaaaatctt ttgtgccgtg
     2641 acagaatcga tcccctgaat tccccgaagc cgctcaagat tggcccgttt caaatcctcc
     2701 agagtgtcaa acgatttcag gaggattcgc ttccgcctgg ctcccacccc atcaatctga
     2761 tccaggaggg agctctcgag ctttttcgac ttgagtgaac ggtggtaagt gatcgcaaaa
     2821 cggtgagcct cgtcgcgaac tttttcaagc aggtgcagaa ccggggtccc cgcgggaaat
     2881 ataatcggtt cccgaaactt ggggctgaac acgtgttcga acctttcggc gatcgcgatc
     2941 atctcgattc tttccatacc ttcctcccga agcaccttga gagcggtatt aagatgacct
     3001 cgccctccgt caatcatgat cagatcggga atgaaagact cacgtccctc cctgatgccg
     3061 cgtaacatcc ggccaagagc ttcccggatc atggcataat catttatgcc cgtcacatcc
     3121 cgaatcttgt accgccgata atgtattttg tcgggaagtt cccggaaaaa gctgactttg
     3181 gaagcgaccg actcatcccc ctggatattg gaaacgtcga agcaaacgat tttcgcggga
     3241 cagacggcca tcttcagagc gcgtttcaga tccatcgtgg ctgaaagtcc ggccccctct
     3301 tcctttctcc ccgggaagaa tcttttcctt ttaagctgac cgagcgcgac gatctgctct
     3361 ttaaaaaact gcgcgtcctc aaacctgagt tcgcggcttg cccgcttcat ccgatcggtc
     3421 aaatattcaa gaaaacttcg ctttcctccc cccagaaaat gtctcacttc acggatcagg
     3481 cggtcataat cccgcttcac ttcagggtgg acgcaagggg caaggcattg gcggatgtga
     3541 taataaagac aggcgcgctt tggcaacgcc tgacacttcc ggatcgggaa aagattatgg
     3601 atcaacgcca cggcctgccg aaggagtttg gaatcggtat aaggtccgta ataaagggcc
     3661 ttccggtccc ccttttgccg ggtgatcgtc aatcgcggga actcgtctcc cgtgatttta
     3721 aggagcggat agcttttgtc atccttaagt tctttattgt agcgcggacg gtattttttg
     3781 attaactggg cttcaagcaa aagagcatcg acttcgctcg gcgtttcgat gtgatcaatc
     3841 agacaaacct tcgacatcat gactttgatt ttgggaaggg ccggagaaac agaaaggtag
     3901 gagcggacgc gtttgtgaag tgattttgcc ttcccgacat agagaatctc gccggaacga
     3961 tccttcatca ggtagacgcc cggcgtcgaa ggaatttttt ccaccatttc gcgaagcggt
     4021 tcagctgata ttttcttcgg catcatgccc ctctggcaac tggttatcgc gtctcacaac
     4081 tcaacacgcg cggccgagcg atcgcggcca cacccgcccg ggcccgcgct atttgcgctt
     4141 tctttttttt tggatccatt cgaccgcctc atggatctcg ggaacacgaa acagaaaaca
     4201 aagcgcgaga tacgaaatga tcgcgatcag aatactgccc agcacctgaa taccctgggc
     4261 cagagtatcg gtccccggga caacggaacg aaggcccgcg aggctagcgt agcagattcc
     4321 cgccatcgcc gccgacgcga gtaaaattcg aaaaaaggag cgggcaattt cccgaaacgg
     4381 aaattccccg acttttttcg gataataata gattaactgc ccgaactgga ctattcccga
     4441 aatcgaagtc gcgagggcga gcccggcttc tttgagtggg atcatgagaa ttaaattaag
     4501 gactatgttc aaaaagagag aaacgagagc ggtgcgcatg ggcgttttcg tgtcgtgagc
     4561 cgcgtaataa ccggcggcca tgatcttttg tcccgaatag gcaaaaagcc caatcgtgta
     4621 acccagaagg accgcggcgc ttcgggccgt tgaaaccgga tcaaattctc cccgttcaaa
     4681 gagaaaccgg atgatttcct cgcgaaaaac aatcaatccg accgaggatg gaatgatgat
     4741 aaaaaaaaca ctcctcaggg cgaatgaaag cgaccgcttg gccgcctccc gttctccgct
     4801 ggcaatctgc tggctcaaca tcggaagcaa agccgtcccc atggcaagcg cgaagattcc
     4861 gagcggaaac tgcatcaacc gattcccgta ccagagactt gagttggcgc cgggaccgat
     4921 cacaagccca agcgtcgcgt ccaccgtaat attaatctga accaccgcga agctcatgac
     4981 aaccggcaaa agcaatttcc aggttttctt gagcccctca tgggcaagat cccaaataaa
     5041 tttaaagcga aacccaatct tccagagcgg aggaatttcc accgcgacat gcaggaaacc
     5101 cgcgaaaagg agtgtgtagg tcaaccatcg tatcctgtcc gcgtaatccg ttccgaccca
     5161 cgaagggacc caaaggatcg cgaaaatcca gaccccgtca agaatcacgg agcccaggga
     5221 aggcgccaaa aagtggccgt ggctattgag aatccccatg ccaagagcgt aaagggagag
     5281 aaaccacaga tatgggaaaa gtatccggag aagattcagc gtcaaagtca gcatctcgga
     5341 ataatgcccc gcggccaaaa tcgccgtaat gagcccctgg accgcgatca caaaaaccag
     5401 aagagcgcag gcgagaaaac agagcgtcac attggcaaac cgaaaagcct ccgcgactcc
     5461 ctgttttgtt ttcgtctcgt tgtaaaccgg aataaacgcg ctcgtgagac cgccctcccc
     5521 cacgattcgc cgaaacaaat tggggatcat gaacgcgtaa ataaaagcgt cccactgcca
     5581 ccgcgtcccg aaagttttgg cgcttaaaat gtcacggatc aatcccagga cacgcccgag
     5641 cagggtcatg gcgctcacga ggacggccgc cctgaccaga ctttttttcc caaatcccgt
     5701 cttcggaact tcgttgacag gacgaggtcc acgtgttaca ttgtcgccac ttttttcaac
     5761 agaagaggac atagtcatgc ctaatataga ttcagcagca aaacgaattc gcagtgacgc
     5821 gcgcaaacgg gccagaaacc aagccgttct ttcggaaatt aaaactcttt ccaaaaaact
     5881 ggttcaactc accgcgagcg cggataaggc ggcggctgtc gcccaggagc ttatttcccg
     5941 ctatgaccgc gcggttacca aaggcgtcat tccccggggc cgagccgacc ggcgaaaatc
     6001 gcgtgttacg aaatttgtca cgaagctttc cgcgaaaaag taagtttccg gtcacgacac
     6061 aaattccata atccaggttt cagtccccga gacggggtga gccagtcctg tctttgtttt
     6121 tctgtcgagt tgatgcaaag cctccagagc ggcttccagt ctagccaccc cgtaccggtt
     6181 gatcgcggac ataaaaggcc ctttcttaaa caacgggact ttgacctttt cgagaatttc
     6241 cgcttcgctc gtccctgcct cccgaagcac agccccctgc cacaattgcc gcaattgcca
     6301 gtgaatgatt ccgatcaaaa agcgcggttc cgattcggaa ttttccagaa gttcatgaaa
     6361 cgtctgcctc gccttcccac gatcacgctc caaaatcgca tccgcaagct ggaaaacatt
     6421 aaccccacgc cagttttctt cgaacaaaca aaccatgttt tcatctatta attgcgcctc
     6481 tcccgaatac tgtaagatcc ggtcaagcat ggtgtcgaga aacacaatcg cgtcaccgca
     6541 catttccaga atccgttgcc tggcccccgg agtgatggtc tttttgaatc tcgctagttt
     6601 ttgctgtaaa tagttctgcg ccagcgaccg agcttcttcc tccgcgagac gaaccacttt
     6661 ccctcgactt ttcgctaggt tgataagttc ggagtgtccc tccgaggaat cgatttcgaa
     6721 aataagacag gtcttttcgt cagggctcat ggcgtaacgc ttgagaatct ccatttctcc
     6781 ctgggttaat ttttcagcga cttgaatata aaaaatctgc cccgaagcga gaaagggtaa
     6841 cgtcctggca ttagaaagga tgctgtccag cggggtgtca gcgaggcgat acgtttgatg
     6901 atcgaaagat cccttggctt ttttttcaag gtcggaaacg atcgatttga tcttttggga
     6961 acgaaggaaa ggatctccga taatcaaaaa aaaacggctt gtcatcgcgg ccctcttcgg
     7021 gaggtgaaat ggaaaatcat tggcgagaga atactggtga agagaagacg gcgtcaccag
     7081 tcttcaacga tacgatccac aatattcttc gccagcttgt caacggcttg ttcaacggcc
     7141 gcctgttccc caatctgttt aatatccgtc acgtaatatt ccctggcgcc cgaaaaatca
     7201 ggctcttccc agataatctc gttcgttttg gcatccacca gtctaagatg cataacgata
     7261 aacatgcgat attcttcgac ggattcaagt tgggtaaaac gaagaccttc ctgctcgtat
     7321 atcttcagat ccatctgcag gatagaatcg gcttgcccct cgggcgccac gcgaagattt
     7381 ccgtctattt ggagccgggc gatcacggca ttcgtgatgt ccatttcaag gccctgctga
     7441 taagagcgca tctccaggat cgggatttta tttttaaccg tttcgacata gatggttttg
     7501 atgtcgcgcg gaagcaccgt cttggtcgtg taaccgcacc ccccaaacaa agggacgatg
     7561 atcacacctg ccaaaaaaat ccatataaac cgttttgcaa atgtcatatg accgctccta
     7621 taaatccttc ttttgataac gttgttgctc cttcataaga aaatcatggc gagtgtccag
     7681 atccgagatg cgctgttccg cgcgtttttt ttcgtctagg atttcttgcg tcatcgcttt
     7741 cgaagtcacc ccgggaagaa tcttgtccgc ctcctgtatt cttttctgca ggacttcttt
     7801 ttcttccgat tcgatcttga gttcttctcc aatcaatttc accaaagaat tttcaatccc
     7861 tcgaaattcc ttataccgct ttactctttc tttttccgcg ccgggagcat ccatctcttt
     7921 ttccagtttg tcgcggtagt ccgcggaaag gatcccctgc tcttcagcat aaatactgta
     7981 ctggacctcc aattgctcaa aaagccgctg tatctcttcc cgaagcatct tctcccgatc
     8041 ttcaaccgaa gcttgatccg gctcggccgc cgttgccgcc acggtcgacg atcttgaacg
     8101 aatttccgaa tccacccgct caatctgctt ttttgccgct tcaagttcac ggctctgcga
     8161 atcaagacgg ttcagcaagg tatccctgcg ttccccggag ggaagaatgc gtctggcgtt
     8221 atgcacggcc tcatcgatat actcatacgg caccttgacc agcgaactcc ggaacttgta
     8281 tccgccggcc agtgacgctt ttgccgtcaa aatctcatgc tgtttctcaa gccgcgcgag
     8341 ttcaatactc tgggcttcaa tcaccagact ctccagttca acggcctgct gaagctcctt
     8401 aaccttctgc tgggaacttc gcttggcctg tcgatttaat tgctcatccc gattcggaag
     8461 cccgaaaaca aaggaacgcg cgaggtacgc cactcccgtc accgcgtttt tcgtcttccg
     8521 aaaagttgtg tgacgatccc cgttcaggac ctgatccagt tcatccagga gctgtttctt
     8581 cttttcgtga cgatcctgaa tatcctgata cctctgccgg atatcttttt cgacggattt
     8641 tatctgcttg cgaagttttt tgagatcggc atcatcccgc gcgtccagtg attttaaaat
     8701 ttcccgctgt tcctgaagcg ccaattcgcg gttaaaggca tgggagagag tatccaccac
     8761 gttctgctgt gcccgaacct tttcagccag gtgcattttt agcgcgagca attcatccat
     8821 ggattttttc tgcacatcgc cgccgacatc aaaatgaacg gggccccccg tctggactcc
     8881 cgcctgacgc gcgagaaccg cctcaagttc ctgttcggcc aactttccga aaatcttctc
     8941 atactccgcc agtttcccat caagcttttc atcaagcgcc ttaattgacc tttccctgtc
     9001 gctcagctga tcggaaggtt tttcggaaat ccgctggcgc tcggaacccc gaagccgctt
     9061 aatttcaaca cccgtctcgg taagctgggg agcgagatcc gcctggagct caccatagcg
     9121 cttgtagagt ttctccttct caagaaaaag gtcctttttc gccttggaga ttttgtaaag
     9181 atctttttcc cctgtttctc gaaccttttc aacgggtgat tgaggttgca tgaacgacag
     9241 aagattctga tagaaaggct ccgaagacac gcccagctcg gctctccagt cggcaagacg
     9301 cgctttctcc tccaaaatca ggtcccgctc ggcctccaga gaagcaagcc accgatccat
     9361 ggcccgctgg aaatcggggg acgtgttgtt ttgataacgt ttcttcttga gttcaaacgc
     9421 tttaaacttt tccttcaact cccgctcccg gcgtttcaaa tcctgtttcc ggcgaagcaa
     9481 ggcatccgac ttgctcccgt ccaccgcgtc cagctttttc ttcagcgcct tgatttcccg
     9541 ctcaaggtct ttttgttttt gcggatcact ctttccaatc gccgccagtt ccccttccag
     9601 tctcgcgatt tcagttttaa tttcatcaga cttcgcggtg agataagtga cgggctgttc
     9661 gagggatttc agtttgtcag cggctttttg tccccacgcg gtgtcggggt accgcttgac
     9721 cgtatcgcga taataaatga gggcgctttc cagataattt tctttttcgt aataagcgcc
     9781 aattcggtaa ttcttttcgg cgttccgttc atcgacttca cggcgaatct gagtcgcccg
     9841 gtcagcctcc gccgaactcc cgtatttttg gagataacgc tccacctgtt gcgacacgtc
     9901 gtcaagcgcg ctttgatctc ggatttcaac ttgtgatttc gcgtaagaag cttccgccat
     9961 ctgcaaccgg gcgtcctgaa ccagggggct ttgcggatac tggtcaatga cctcctggaa
    10021 agcttccatg gccgggtcat actgccctga tttcatgtag gcgttcccaa gctgaaactg
    10081 cgccttatct ccaaaggcgc tgaagggggc cgcttccaca atgcctttaa aaatatcgat
    10141 cgccatcgga atggaaggcc tgattttcag tccgagcaac accccttttt ccccggaaag
    10201 aaaagcggag ccaatctcgt attcccgttc gactacttct tcaaacctct cgctttgcgg
    10261 atacgtcttg aggagatttt gataagcctt gtgcgccttg accagatcgc ccttttcttc
    10321 atgaatcgta cctaaccgat actgggcctc aggggcaaca atagatttgg ggtatttttt
    10381 gaggataagt tcaaaacctt cggccgcaga gtccagcttt ttttcttgaa aatagtccag
    10441 ggcctggtca aatgcgtcct gggaatcatc ctgcccgcct tcgtccgtac tgacaaactc
    10501 gcctcgttcc ggagaccaaa cccagtccgc gctcgctcga ggtcccggca ccaggacaag
    10561 agacaccgga atgatcaggg caacaattga agaaaatttc ataaatggtt tattatcttc
    10621 aggttagaga aaatttgagt tggtactata gcacgcggca ctataccggt caatcagaaa
    10681 tcccctgttt ttcctagact ttcaccaggt ttttcgtcag aaatcccgtc tcaaaaacac
    10741 gaatatttaa agcgcctttt tcattcagga aatagcgctg gccagcctgt ttctgaagga
    10801 aaagagccgc ttccgtttcg gctcccggaa gtacccataa tcgaacttcc tcgacaccat
    10861 tccacggcgc gatctttttt acgaacggca agattaaaat atccggagga ggagaaattc
    10921 ttgaaatcct caaggggtca agcccgcacg gcgcaaaaca tatcttctgc tggcctgcct
    10981 cgataaagac ggcgttatcc ttttttccgg cccactccat catcaaactc tcacgatccg
    11041 gaagctccag gattgtttcc ccacttctga cgtatcgaaa cttatccgaa tagcgggaca
    11101 gttcctcccg gacgtgaaaa tcttgtggaa aaataaccgt ccggactctg agttcccgga
    11161 gaatcgtcat gagaccgccc cagtgttttt tatatgatcc cggcaccaga atcccgtcca
    11221 gcgccctgat ccccttctgc tgaaagtacg gtagaataat tttttctccc tgatcggacg
    11281 ggaagcggcg accggcgccg atcagccagt tgcgacctcc cgaaaaccgg acatgaatga
    11341 ccggatcacg cccggcgcaa agaacattca cgaaaaatcc ggcgtccggt tcggttcgca
    11401 tcatggttaa tcccgtcacc agacaaattc ccgcgcaaag tattgccatt tgttttttcc
    11461 ataacgaatt ccgcccccag aaaaaaagca aaccagccag ggaaccataa taaccaaaga
    11521 gttgccaggg cgttggtttt gaaagaaacc aatagcccca tgtcaggcgg gaaagttgga
    11581 caatccacgc gctcatcagt tgaatgaaag cggaagagat cgcaattaac cactcgccaa
    11641 gcaagggaac ggctgaaaac agaagtgaaa aaagagatag aaaaagagcc gcgtcaaaaa
    11701 cgggtatcgc gacaaaatta gccatcaggc ttaccggtga aaacacatta aaataataaa
    11761 gaacaagcgg gaacgtcccc gccgtcacga ctaggctccc tccgagggaa cccggaagag
    11821 aacgacatga cgagaatcgc ggaagaaaag caattagtga aaacacgcaa aggtaggata
    11881 gttgaaaccc tacattccaa agatcccgcg gtctggcgag aatcatcaaa aaaaacgcga
    11941 cacacaacgc gttcatgctg agggacggcc ttccccagat aactcccaaa agaacaatcc
    12001 ctgccatcca gcccgcccgt tgggcaggca gtcccatccc cgagattgac acataacaaa
    12061 ggacgacggc catcgtcaac attgccgtca tccgtcgccc ggcccccaac gccagaaaca
    12121 gaagataaaa tcctcccgcg accatcgtga tatgaatccc gctgatggag agaagatgaa
    12181 tggttccggt cttcacaaac gcgcttttca ggtccgaagc aatctcgctt cgaaaaccca
    12241 aaatgaccgc cttcagcatc gaagattcag gccgttggta aagcgcgtca atttttgagg
    12301 caatcgtgag acgtaatttt ccgatccatc tcgcgggctg aaacacggaa ccttccgcga
    12361 caattcgtcc cgaattcggc ccaatcactt cgatcatcgc gtgaatgttt ttctcggcaa
    12421 ggtaccgcgt ctgatcaaat cctccggggt tttgggcctg ccttggtttt ttcagcaaac
    12481 cataaagacg caccttgtca cccacggagg gaacagtcgc cggctgaatc cagtacaccc
    12541 aaacttgtcc cctcaccgct cgtggctttg gatatcgccg ggaaccaaga ttgacttccg
    12601 ttgatttcag gacaaacgag atatttctgc cccgtccgcg gcatttaaca tccggcaagg
    12661 tcgcgacgat ccccgtgaca gcaagccggc ggtctgatac gaattgatgc gcgctgtttt
    12721 caggttttaa tccatcaata ctaaaaccaa tccatcccga ggtgaatata aggattagaa
    12781 aaacagggaa aaaggatttt cgccccctca ggaaccaggc gaagggtagc aaaataacaa
    12841 gcaaagaaac aaccacgggc gtgaccgatg gagcgtaccg gctcgacacc attcccagcg
    12901 acaggcttat ggctaacgga agaaaaggaa atttcatgat gatgattatt atacaacatt
    12961 atattgcata ataatgatat ttttaaaaac ttcttccgaa acaagcgact gaaattggga
    13021 ttaccttttc aggaaatact tgagggcgag aaaaaaaaga accacaagaa gcagaagaaa
    13081 aaagaccgta tgcgaagttt ttcgatcgtc agacatcggc tcgaaaagac cttatagttt
    13141 taatcgtaaa acttcccctc gagattccag cgtcacttcg tctggatcaa tcttcataac
    13201 cacccactca ttccccacca aatccccaac cgccaaaatc cggtcatcga tcacggcacg
    13261 agccccctcg ccaagatcca gaatacccgt cagcttcaac gcatgacgac tttggtgaga
    13321 caagttttta ttgctcatga ccggcacaat cttcgccagg gatgaaaaac cggcgcgggt
    13381 gtttccgtcc ggatctcgca caccctcggg aacagccgaa ttcaattttt tcggaacacc
    13441 accccccgtt agctctggcg acccggtatt tgaaacccgc tgaagaatcg tgccaagcac
    13501 aaccagggaa agcaaaataa aaacccccgc caggatcttc cacccaaggg cgcgccggga
    13561 accataatga cgctgaacct ctcgaagctc acgctccagt ctttcatcca tcaccttcgg
    13621 ccaatcaggc aatggtttcg atgacgtctt gatctttcct gccgtctccg gagacggagt
    13681 ttccatcttc tgcttctgtg atgaagcggc cttgttaagc gccgcctgga taaggctcat
    13741 acggcaatcc cctgcagatc agcttccgcg cactccacca tgggcgcgtc aatccgttgc
    13801 gcccccgccg cgaaggccgc aaggagagcg cggtccgcta ggatattgat cacccgcggg
    13861 atcccccctg aaagctcgtg gatccggcca atggcctcca gcgaaaaaat atcgtcggtg
    13921 gctcccgcga ccttcagtcg atgcctgatg taaaggccga cttcctcgag actcaattcc
    13981 gggagatgac aacgtaccgc gatccgctgt ttcaactggc gaagatcacg gcgtttcaaa
    14041 aggtcattaa gttccggctg tcccattaaa ataatctgta gcagtttctg ggacgcggtc
    14101 tcaagattgg aaagaagacg aatttgttcg agcgccttcg gcgggagcaa ctgcgcttcg
    14161 tcaataatca ataccgcgtt aaatcccttc gaactttcct caagcaaaaa ccggttcagc
    14221 gcgtcaaaac aggcttttct cttttttgaa gccggcttga ggccgaaatc ttccacgatc
    14281 acttcaagca gttcaatttc cgaaagcttg ggattgaaaa cgagtgacgt gtggacgttt
    14341 tcgccaatct gctggaggaa cattcgcgaa agcgtggttt ttccggctcc aatctcaccc
    14401 gtgatctgga taaaccccac ccgccggtcc acgccatact tcatggagga taacgccgac
    14461 tgatggtgcc ggctgagata cacaaaccgg ggatccggcg tcacattgaa aggcggttcc
    14521 gtcaggtgga aaaatgattc gtacattgcg tctcccgtca cttaatgaac ggcacatacg
    14581 gtcttcgtct tgagaaaaac aggaaagtgg gatcaggcct tgcgaaaagc aatttcaaaa
    14641 acgcggccat ccttgcggtt aatgggaccg ttaaaaaccc tgcgccgttc ccccgtctgg
    14701 tccagtttct cacccgtgat caggtcccgg acgatcatat ccccccggca tttttcaagg
    14761 gcgggaagaa acacggtacc gcttttgtct tcattcccgt tattcaccat gaccagataa
    14821 tgttttgctc cccttctgaa aatcgccgtc ttcacatcgc gggtcaccga ataagcgaaa
    14881 agcggcacgg acagaaaccg caaaactcca aggaccagtt cgggcgtagg atccacgccg
    14941 aggtggatca cccttccctt gccaattttt ttgatgtagc ccacggtcag gttcccgtat
    15001 cccttgattt cggcctggat tttttggcaa ccatccgcct tgaaaacgta aaccgaactt
    15061 gtgacctgaa tctccggctg tcctttatcc agcctgatct tgaactgacg cttgaactca
    15121 aaaaggattc gttcggggtc ttcaaacccg gtcacctgac agggattaaa attttcgtct
    15181 ttcctcggat aactcacaaa cgagaccagg gccccccccc gctccacgta ccggcggagg
    15241 ttctcgtgcg aaatcctgtc cagccattga ttgcccgaat aaaaaaccac tttcttgtcc
    15301 gggacggcct gtcgggggtc acagacatcg tagtccacgt ccccgtggta gagggcctgc
    15361 aaaatcggat tgtcatgcga aagcgttcgc gccgcgtact ggaggggatt aaaagtgacg
    15421 gcaacttcgg tcaatttttc aagagaggtc ggcctgagcg aatgaaacgc gcgaacgatc
    15481 tgtttaaaaa ccgcgtaaag ttccccacga gtacgccccc attcattgat cggagacatg
    15541 taccagttgt cacgattcac caacatgtac cagttccatc ccttgatgcc cccgagaaag
    15601 gccgacaaac aaatcatccg gtaatgattt ggcgtcagca cgctggtttc gtagtggcga
    15661 ccatgccaga cccccgattg aaattccgcg ataaacggca aatgagacac gctcgacagg
    15721 taacggattt tgtcgcaaaa tttccggtgt tcaaactcgt cttcggcgag ctcattggaa
    15781 ggatacaaat ccactcccac catgtccgac accgactgaa gccgggacca gtcatgcgcg
    15841 taaaaaaacg ggtacgtgtt cagatagatc ggtacgtcaa tcccgaggtt ccggtaggtt
    15901 tcgacgttcc atttggcgac cttcgtggcg tagtcatatt tgaacttgaa gtagtcgaga
    15961 ttgcgtttaa gttctttatc gcccttgagc ggaattccct gcgcgttcgc gagcatggtc
    16021 gcgataaaag gtgacgcctc cttaaatgag gcgtatcgcg ttccccacgc attattaagc
    16081 gcctccagat cgttttcgta cagcctcgcg ataaaatcct gaaaaagccc gggagggcca
    16141 ctcaggccat attgctgacc aaacacgtca ggccacggat cgatctcgtt atccgcttgg
    16201 agcaaaataa tgtgaccgcc ccgtcgcgtc gcgagataag gtttaaaaac cttcgtaacc
    16261 tctttcaggt acacgctcgc gtagtcaaga aacctgggat gaagacggtg atatttgtag
    16321 gcgtaagcag gaaccccgtc atggggccac tcgctgtaga tgtaaggtcc cggccggata
    16381 acaagccaaa aaccttcctt gcgggtcaag tccagaaaag ctttcaaatt tcgcgttggg
    16441 tcggttttgc ccgcaaaatc gaatttcccg cgctgatact catgaaaatc ccacgggacg
    16501 tacgtggaaa taattttcag tcccatctcc ttgacacggg aaagagtatg tttccagcaa
    16561 ttcggattca atctccagta atgcacttcc ccggaaataa gaggaacttc tttgccgccg
    16621 accaaaagtt ttttcttttt aatttctacc ttcggcattt tgcgcaatcc atcattggct
    16681 gacacaagcg tgaacaaatc tcaggaagtc ttcacattca tcgccttaaa tcacgaaatc
    16741 tttacataaa aaacacccgg agacgtcgag cgaacagatc gtacccaatt aaccacgatc
    16801 gcacaagcac tatcccgggc ccaggactag gttgacggtc ctccttccgg gtaccacgat
    16861 catcttgata atttttttct cgccgacgat ttcccggaca cgcggctgtt caagaacctt
    16921 cttccggatt tcttcctgcc ccagttccgc ggaaaccatc attttcgccc gaaccttccc
    16981 cgccacaaga atcgccatct cgtattccga ctggatcaga tactggtcgt cgtattccgg
    17041 ccacggttcg taggcaagcg acccggaatg ccccatcttt tgccagagtt cttcggacaa
    17101 atgcggcgcg aacaccgaaa gaagcaacag gaatttctcg agaacttccc gtgaatgatc
    17161 gggatgggcg gaaatttcat tgacaaaaat catgagcgcg gaaatcgacg tgttgaaacg
    17221 aagcgattcc agatcctccg tcaccttcct gatcgcctga tggagcgccc gaagttcggt
    17281 ttgggaagaa gcgcgcgatt gaatattcgg atgaagattc ccgtctttat ccataaacag
    17341 acgccaaact tttccgagga agcgataaac gccctcaacg cctgacgtcg cccagggttt
    17401 cgtcatttca agcggcccca taaacatttc atacaaacga aacgaatcgg ccccgtactg
    17461 ccgcacgaca tcatcgggat taatcacatt cccgcgggac ttggacatct tctggttgtc
    17521 ttccccgagg atcatcccct ggttaacaag tttcctgaaa ggttccggag tcgaaacaaa
    17581 cccgaggtca aaaaggacct tgtgccagaa ccgggaatac agcagatgaa gcacggcatg
    17641 ctcagcccca ccgacataaa gatcaacggg catccagtac tgttcttttt tcttgtccac
    17701 cacggcatca gggttcttgg gatcgatata gcgcagataa taccagcagg accccgccca
    17761 ctggggcatc gtattcgttt cccggttcgc aaccgctccg cacttggggc acttgcactg
    17821 aacccaatcc cggaccttgg caagcggcgg ttccgccgac ccggtcgggg tgtaatcagt
    17881 catctcgggt aaccgcagag gaagttcatt ctcgggaacc ggcacaatgc cgcattgggc
    17941 gcagtggata agggggatcg gttcgcccca gtagcgttga cgcgaaaaaa gccagtcgcg
    18001 cagtttgtac tggacactgg ctttcccaag tcctttggcc gcaagccagt ctgtgatttt
    18061 cttcttagcc tcggccgtcg gtaagccggt aattgacaca tcggggcccg tggaattgac
    18121 cgaagttccc tctcccacaa aaccccgcca ttcggtttcg ggatcatccg gctgaaccac
    18181 ctggatcacg ggaagattaa atcgcgtcgc aaactcaaag tcacgcgtgt cgtgagccgg
    18241 aaccgccata atcgctccgg taccgtaccc catcatgacg taatcggcaa tccagaccgg
    18301 tattttgctg tcattcacgg gattgatcgc gtaagcgccc gtaaacgcgc cagtcttgtc
    18361 tttctcaagc tgggcgcgtt ccagttcact ttttcgggaa gattcctgac ggtatttttc
    18421 aacctgtgcg cggcactggg gcgacgtaat tttttcgacc agcggatgtt ccggggccag
    18481 caccatgtaa gtggcgccga aaagtgtatc cggccgggtg gtaaacactt tgacttcaag
    18541 gtttgaatcc gcaattttga attggacttc agccccttcg ctccgaccaa tccagtttcg
    18601 ttgcatctcc ttgatcgctt ccgaccaatc cacgtatttg agatcttcga gaaggcgctc
    18661 ggcgtaagcc gtaattttca acacccattg acgaagcgga cggcggaaaa catccttgtg
    18721 cccttttcga tcacaagttc cgtccgatcg cacctcttcg tttgccagaa cggccttaca
    18781 tccctcacac caccagaccg gaatttcagc ctcgtaggca agtccttttt tgtaaagctg
    18841 gagaaaaatc cactgggtcc acttgaaata gtcgggatcg gtggtgtcaa cctcacgatc
    18901 ccagtcataa ctaaaaccca gcatctggat ttggcggcgg aaagtttcga cgtttttttt
    18961 ggtcgtaatc gagggatgcg tccccgtttc aatcgcgtat tgttcggcgg gaagcccaaa
    19021 ggcgtcccat cccatcgggt ggagaacatt gaacccacgc atccttttgt agcgggctat
    19081 aatgtccgtc gcggtatatc cttccggatg ccccacatga agccccgaac ccgagggata
    19141 agggaacatg tccaggacgt aatacttggg tttttgaggc gaacattcat cagggtcagg
    19201 cgaacggaag gtcttgtgag tgagccagta cgcctgccat tttttttcaa tttcgtcgaa
    19261 gggatacgct ttaaatccgc tgaccataat tcctcaaagt tcgaaaaagg tgaggcccgt
    19321 tctgaagctt tcgtctccac gattcgcgcc tgggaaccgt cataagccac ggaccgggtc
    19381 ataaaaaaca atgcgagagg agggagtcga accctcatgg tgtttgagca ccgctggatt
    19441 ttgagtccag tgcgtctgcc agttccgcca ctctcgcggg acatacaagt tccttaactg
    19501 cctgccgtta cgaagccttt ttacctccag ctcgtctcca gaattcccct cttgtagtca
    19561 aaaaccatct ccagtgatct ctgatgacaa gagtaacgga tattaggcga atcctgctga
    19621 tttttcaatg acagaatcac gcgcgcttac ggattaaatc aggagtccac ttttaaaaaa
    19681 ccggtgacag tagcgcgtta agctcctgaa cacttcccga aagaagtttt aaaatcccgt
    19741 ccacatccgc cgcgtttcga agtcgggcgt ggagctcaat tcccggctct tcaaaactgg
    19801 ccaccggctc aatccggaga tttttcgccc ggaaagtaat cttgcgggat cgcgtcgaaa
    19861 aatccttgag aaaagccgtc aaacccggaa atcgccgctg acggacttcg gccagaaaca
    19921 aatcaccgcg ttgacgggga tccagcgact tcgcctccga aatcgctctg aaacttttca
    19981 ggcgtaaaat gtcccggagc gtcgtttcat cccgtttgac aatatccccc agccactcca
    20041 gcgactgaag gcactgggaa ctcgtcaggt gcgcgcgccg atccagaaag cgcgcgaacg
    20101 ccttctgatc ggccggtgaa aatctgagga gaagggcgca ggatttaaaa ggaatttggc
    20161 gccggtccag aaggtttttg atttcatctt ccagggcgtc cgcttttcgg taatattcaa
    20221 gaaaagattc ttcggccgga agtcccagaa gcggaaggag tgtgtcccgt acccgctcgg
    20281 gcctgaaccg gaaatcacgg atcgccttcc ggatcgccac gacacgatcc atatcggaaa
    20341 tctcctgctt ccagttcgag accaggctta tctgaaaagc gtccggatca cttatttttt
    20401 tttcgataaa aattaccggg acccgtttca tcccgagacg acacgcgacg taaagtcgtt
    20461 tgtgaccggc aatcatccgg atcgctccct tttcccttcg cgccacgagc ggattcagaa
    20521 ttccacaacg ccggatcgac ttttcaaggt cctgatcctc aagccggtac cggtaacaaa
    20581 agggccccgg gttcacgccc ttccgg
//
LOCUS       DAOSIO010000005        20305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_2886_length_20305_cov_5.276691, whole genome shotgun sequence.
ACCESSION   DAOSIO010000005 DAOSIO010000000
VERSION     DAOSIO010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 20305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20305
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2886_length_20305_cov_5.276691"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..868)
                     /gene="groEL"
                     /locus_tag="PK590_00450"
                     /pseudo
     CDS             complement(1..868)
                     /gene="groEL"
                     /locus_tag="PK590_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106662.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
     gene            complement(970..1266)
                     /locus_tag="PK590_00455"
     CDS             complement(970..1266)
                     /locus_tag="PK590_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOG23099.1"
                     /translation="MKVRPLADRVLVEILEAEEKTKGGIILPDTAKEEKTEGKVIAVG
                     TGKMLESGKVQPIEVKKGDRVLFGKYSGDDIVIDGTKHKILKESEILAVYDTNK"
     gene            complement(1332..2999)
                     /gene="groL"
                     /locus_tag="PK590_00460"
     CDS             complement(1332..2999)
                     /gene="groL"
                     /locus_tag="PK590_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098456.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOG23100.1"
                     /translation="MAKQLLFSEEARRAVLAGVDKLANAVKVTLGPKGRNVVLEKKYG
                     SPTVTKDGVSVAKEIELEDPYENMGAQMVREVASKTSDIAGDGTTTATILAQAICREG
                     LKNVTAGANPMALKRGIDKAVEAVVEELNKISKPVGRDKKEIAQIATIAANSDTTIGE
                     QIAEAMDKVGKDGVITVEEAKTTATTLDVVEGMQFDQGYLSPYFVTDAERMEVVLEEP
                     YILINEKKISAVKDMLPVLEAASKAGKPLLIIAEDVEGEALATLVVNKIRGTLTVCAV
                     KAPGYGDRRKAMLEDIAILTGGKAITEDLGIKLENVTLKDLGRAKRVTVDKENTTLIE
                     GAGKSADITGRINQIRKQIEETDSDYDKEKLQERLAKLAGGVAVIHVGAATETEMKEK
                     KARVEDAMHATRAAVEEGIVPGGGVALLRAKSVLDNLKLKGDEAVGADIIRRALEEPL
                     RQLAQNAGDEGSVVVQAVMSEKGNVGYNAETGEFEDLVQAGIIDPKKVTRSALQNASS
                     VAGLLLTTETLITDIPEEEKMPPMPGGGGMGGMGGMGGMGGMGGMDY"
     gene            complement(3234..4058)
                     /locus_tag="PK590_00465"
     CDS             complement(3234..4058)
                     /locus_tag="PK590_00465"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOG23101.1"
                     /translation="MSWKTFLGHSKKDLSFSMGKIMGNFLLAIDIGNTAVKTGLCKSG
                     AIFNCKTSKINDVPKYINKFIKSGKYNHLDVLICSVDPKKTSYIKKSMVCHNNAKLWV
                     LGSNIDLKIRHNYKEYNKLGMDRKVGCYGALKMYKMPLLIISYGTAITCDYLDRTGTF
                     QGGLIIPGPAIALKALSKSASLLPTIHFPRKKPGFLGRDTKKCMEYGILEGYGSMADE
                     LARRFKIRYDKKIRVLATGGLSKTIAPHSASIDILDPFHTLKSLALIWREKIQQFP"
     gene            complement(3988..4539)
                     /locus_tag="PK590_00470"
     CDS             complement(3988..4539)
                     /locus_tag="PK590_00470"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOG23102.1"
                     /translation="MISFRVEHLREATSTNTLALERARKGEPEGLVIVAEHQTAGRGK
                     PGRTWESPERKNLLASILLRPPITPAKAPMLTQIGCQAVAQTLETHYGIPSTCKRPND
                     VMSDGKKICGILTESITTGPHLEAVVMGIGLNVNAEINELPSGATSMRILKTRNYEIR
                     NVLENLLGALKERLIIFYGENHG"
     gene            complement(4595..5728)
                     /gene="alr"
                     /locus_tag="PK590_00475"
     CDS             complement(4595..5728)
                     /gene="alr"
                     /locus_tag="PK590_00475"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOG23103.1"
                     /translation="MQNTAGSTTVKHQYASWIELSRNHLLANLETIRARSGTPDVLAV
                     IKANAYGHGLREIAQILEGKVKFFGVATLKEGLSLKEHFPKTSVFLFGHLLPHEIPVA
                     VGSNITMSVSSFAQARAISDCAESLERKASVHLKIDTGMGRMGFRADEAVREIMKIRL
                     LQGLLLDGIYMHFPTAEVEDLFRTKQIQKFQSLLEELDRNGIAFRFRHAQNSAASLTL
                     KNSLLNIVRPGLALYGIHPDPSLRDTVKLAPVLELKSRITFIKRIESGETAGYGRTFT
                     ASSTTNLAILPLGYSHGYPWSAWKNAQVLYRGNRYPLAGKISMDYLTVNLGRQEAAEG
                     DIVTLIGKDQDERISAEDVAQWAGSIPYEIVTQLNANLPRVVA"
     gene            complement(5760..6470)
                     /gene="tsaB"
                     /locus_tag="PK590_00480"
     CDS             complement(5760..6470)
                     /gene="tsaB"
                     /locus_tag="PK590_00480"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOG23104.1"
                     /translation="MNLLAIETSGHSLSVAIKKGRAPARHAFLKGFSRHVENLIPLID
                     RLLKKERLKIKEVDTFLIGRGPGSFTGLRVGFATLKGFLAVRERPCFGAYSFDLVAST
                     IPRSGSRCASGENLAVCLNAKRDKLYARLYRSYRQGWKPAGAPFVKAPDDFLKELPAG
                     TRVAGDGLAVCDRDLLKKIHSLPEKYWTPRASALINLYEKKDPLLKPFKRPKDFLPFY
                     LRNSEAEERLKNKTQNQK"
     gene            6793..8415
                     /locus_tag="PK590_00485"
     CDS             6793..8415
                     /locus_tag="PK590_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG23105.1"
                     /translation="MISTNGITLQYGKRILFEDVNIMFTPGNCYGLIGANGSGKSTFL
                     KILAGLVEPNAGTVTAGKNERISFLRQDQFAFDEFTALSTVIMGHERLYNIIREKDNL
                     YAKSDFSEKDGLRVAELEHEFSEMNGWDAEPEAAKLLSDLGIPDARHQVPMKQLESNE
                     KVRVLLAQALFGNPDVLLLDEPTNHLDVETCLWLEDFLYEFKNTAIVVSHDRHFLDRV
                     CTHIADIDYGKIQLYAGNFSFWVESSQLALRQRTETNKDIAARRKELQEFIARFSANA
                     SKSRQATSRKKLLEKLTLEDIKPSSRKYPYIAFKAERDSGNDLLEIEGLEKSIEGEMM
                     FTGLDLQVSKGDKIAFLGPLDRVKTTLFQVLVGEIKADRGSFKWGASTTRAYYPKENG
                     KYFETDLNLVDWLRQFSKETDENFIRGFLGRMLFSGEESLKKASVLSGGERVRCMLSR
                     MMLVGANVLILDEPTNHLDLESITSLNKGLENFKGTVLFSSHDHQFIQTVANRIVELT
                     PRGIIDRRDMTLDDYLADPGLKVQRAALYAPA"
     gene            complement(8481..9443)
                     /locus_tag="PK590_00490"
     CDS             complement(8481..9443)
                     /locus_tag="PK590_00490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006891434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOG23106.1"
                     /translation="MIQTSVIIPTYNRSAMLSRAIRSVLAQKGARFELIIVDDGSADG
                     TNERDIQRMARGEKTPDKNSAHRHPSAVQIRYLFQTNQGPSAARNFGIKESRGEWIAF
                     LDSDDEWLPGKLRAQLDFFEQNPNFRIGQTEEIWIRNGQRVNPMKKHKKYGGWIFERC
                     LPLCIVSPSAVMIHRTIFNEVGFFDESLPACEDYDLWLRIASRYPIGLIDKPYIKKYG
                     GHAGQRSREFPAMDRFRIRSLAKILKEGILTPEQTMAARKTLEGKARIFIQGALKRGK
                     KKEAEEIASIVEQTRSTGPSQPKSPTNHFKIDSGATPRSGSFKC"
     gene            9522..11075
                     /gene="glgA"
                     /locus_tag="PK590_00495"
     CDS             9522..11075
                     /gene="glgA"
                     /locus_tag="PK590_00495"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HOG23107.1"
                     /translation="MMSKLMTAQKKTTKDNPRNSVTKTDLSRKLKILFIASEAVPFVK
                     TGGLGDVCGTLPKALKKLGHDVRLVLPRYWAVDRYAWKLTTALAPMGVEMGDRTIWCE
                     ALKGKADGFTVYFIEHEQYFGRAGLYDDGNWEHPDNLERFGFFSKAALQLAKDIKFRP
                     DIVHAHDWQAALAPAYLKTVYKNDPFFKKTASVFSIHNIAYQGTFKSDQYGFLGLGAE
                     NFRQEVFENYQGINLMKGAIFFADGISTVSPTYAREILSEPGGNGLSDYLNRRREDIS
                     GVLNGADYDHWDPAVDKLLPARYTADDLHGKTICKRVLQEEFQLDIEPKAPVIGVISR
                     FTQQKGFQNLMPVVANLFQETPAQMAILGTGEKWQEDFFRGLPAAYRGRAGAWIGYSN
                     RKAHLIEAGADFLLVPSLYEPCGLNQMYSLRYGTLPIVRATGGLKDTVVQYEESTGAG
                     TGFLFYDATPGAILETVKWAVRTYLDRPKHIEILRRRAMRQSFPWSDSAKHYEAIYQR
                     ARKRRLMWL"
     gene            11291..11914
                     /locus_tag="PK590_00500"
     CDS             11291..11914
                     /locus_tag="PK590_00500"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOG23108.1"
                     /translation="MSQSTKAYDKLSLSEYLAKLSSDSPVPGGGSVSAYVASLGMGLT
                     QMVARIALNRKPKAGATPEQKFQHEANRKRMEEIISSLEKAKAQALQVVSLDPQVYDE
                     VMACYRENAQPEKLEDALEHAFRLQADLALIIEMAREWNTHMKGLVQGAIANDLVVSE
                     RLLAAAFEGAYHTARINVVYMKNQERKARAEQALEELNRRYQQGRES"
     gene            11917..12774
                     /locus_tag="PK590_00505"
     CDS             11917..12774
                     /locus_tag="PK590_00505"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOG23109.1"
                     /translation="MESKRLEGKLIAAEIQTRIEKTVREILTRGASRPRLVSIQTSED
                     PSAVWYVGQQEKLAAKLGIDFQRISPQEVLTEKALIEKVQSLSADPKVHGLFISMPLP
                     EGFDADKVLLAMDSRKDVEGIHPASLGLIMLRKGKLIPPTAYAAFSLIKSTEIMLRGK
                     KAVIIGQSAIVGRPLQLLLGEARVTTLVCNTGTSEEDIKKLVGMADIVIGCAGKPGMI
                     KGEWIKKGAVVIDVGTTEVEGKLVGDIEFEAASLKAAFITPVPGGVGPLTVTMLMKNL
                     ITAYEWQNK"
     gene            12855..13643
                     /gene="panB"
                     /locus_tag="PK590_00510"
     CDS             12855..13643
                     /gene="panB"
                     /locus_tag="PK590_00510"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010505103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOG23110.1"
                     /translation="MKLTPDDIYSKKARGEKITALTAYDFPTARILDGAGVDVLVIGD
                     SLGMVLLGDETTHQVTVSDVAYHTKAVAKAAQRALVVADMPYRSYLSPEEALSNARIL
                     VRDSSANAVKIEGGVAIEEQIRHLVRNGIPVVGHIGVLPQSVEGKKYRVFGKREPEAE
                     SIMKDAKLLESLGVFAIVLECVPGTLAARVTAEVKCPTIGIGAGASTDGQILVVHDIL
                     GIRSSVSPRFVRRYADLENVIKQAAEGYCRDVAAGTFPSQKESF"
     gene            13646..15103
                     /locus_tag="PK590_00515"
     CDS             13646..15103
                     /locus_tag="PK590_00515"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG23111.1"
                     /translation="MSKGKKILVIEKEEEARELMVMRLESRHHKVIQASSTPLAMRAL
                     EREVLDLILLSTDMEPVDGKPLLQVIRSRSHLMAVPIILLAKESNLTELMLGHEKGYD
                     DFIIKPVNPLVLQIRVNLSISKTQERVEANALTHLPGNHAIEKIVSKKIRAGEKFSVL
                     YIDVNHFKSFNDRYGFQKGDDVILQTARLLVATSSAVSSAGECFVGHLGGDDFIVVTP
                     PELEDVFARQFLDDFDSVMPTYYSKEDREAGCIRVENRRGEMENFPLMSCSVAACTNL
                     YKNYTSLREISQDAVEVKSFLKTQPGSHYLQDRRSMPIQKIDEAVHILAPEIKASARR
                     HKKSRVDPLGQVLLSAGLINESQLSEALKKHFETGQRLGQTLIGMNLVPSHEVGKMLE
                     QKLKLPYFRLKDWSPSPELRELFTGDLIARHRVMPVEVTEGAVKLAMCDPFDIRTLDM
                     IERITGLKPIPCLALEDEFEVFLEKNFPSQPVDIN"
     gene            15279..15614
                     /locus_tag="PK590_00520"
     CDS             15279..15614
                     /locus_tag="PK590_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23112.1"
                     /translation="MGIESFPEIENFQKLPREVIIKGASTKFGREHGAELRGIVINNI
                     GQPIRNIRAQLVVFGKNKVPVLGTSSAADPDKLPQGGIAKFRFFLKGFKQDLKNYHLR
                     VVWNFDESS"
     gene            15785..16015
                     /locus_tag="PK590_00525"
     CDS             15785..16015
                     /locus_tag="PK590_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015163527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HOG23113.1"
                     /translation="MKTTTEIEMKAIKSRDIALVGYDAGAEILEVVFRAGGVYRYQNV
                     PEYVYQELISASSPGTYFQHYVKNTFSYVKVR"
     gene            16031..16459
                     /locus_tag="PK590_00530"
     CDS             16031..16459
                     /locus_tag="PK590_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23114.1"
                     /translation="MVLRIKTLLVVFCLLFPASGWVHARLPIRESEAFQQYLKKPRGE
                     FAKLVCLLNYYRDGDFIIRFDGFDYVPSFAFPHAKAWLFTHYNKDTASQWIRKNCYVS
                     PFERKVIYVKFPDGELEPARDMILRDLAELERAIAEVHAV"
     gene            16449..17147
                     /locus_tag="PK590_00535"
     CDS             16449..17147
                     /locus_tag="PK590_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23115.1"
                     /translation="MRSEIPRSENMIPETRSTVKIFLLTVFVICFWGAEVQAETPFKV
                     TVKPDQSSVQPGMMFLVNAGVTNRTENTVILQVPSCAFDKLWEVDGEHIRIQPWTCHQ
                     NTTERAEISPGESYEKNLILYASDVNQPGPVTFRMGFKHVLENGDEMEPVWSDPVTIQ
                     ILIPEGPLKESALPGEEARPISQSPAGVEISEVMPDEISEDEVFGIEGESPTSQHRDE
                     SFNVPDMDPAQSGV"
     gene            17165..18502
                     /gene="hemG"
                     /locus_tag="PK590_00540"
     CDS             17165..18502
                     /gene="hemG"
                     /locus_tag="PK590_00540"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HOG23116.1"
                     /translation="MIPKVVILGGGISGLVTAHELARCSPAQITLVESSTRLGGVIRT
                     LRPEFFEMEAAVDTLDGKNGSVLELCKDLELESYLVPRESALAHFFLKRNREIFPVGF
                     SPEIFKNNALTLATKMRMLGEVMIPRRKDAMDESVGSFIRRRFGNLFFTQIVQPVIRG
                     GMMADPDDLGVGEYFKRWVEAESRFGSLIRAVLSGKFPGTARMTAFSVRGGMDRLVAG
                     FLKRLPEIHVLLNSRAVTVRKDQSWIAELEDGTRLSAGAICLALPAHESSRVLDKNFS
                     DLSRDLEKINYETVAVINLVFPAQGFPREFSKTGFFIPENGRESPFSSVKFCGPEETG
                     AWIRMKVFIAEKFHREVFLLEDKALLEKVARNLEEQWGFPEPFWAQVERYWKAIAQYP
                     PGHRNLVREVDSRAQRHPGLFLAGNGYRGFGVPDCIERARETARRIADFLNRK"
     gene            18658..19239
                     /locus_tag="PK590_00545"
     CDS             18658..19239
                     /locus_tag="PK590_00545"
                     /inference="COORDINATES: protein motif:HMM:NF015743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017128 - phospholipid scramblase activity
                     [Evidence IEA];
                     GO_process: GO:0017121 - plasma membrane phospholipid
                     scrambling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid scramblase-related protein"
                     /protein_id="HOG23117.1"
                     /translation="MDVLKSIDSLVIREKKEYTGVPSTWKVRPEFCLSDSSEKECFYL
                     FSEKGTAQGFPALQESKSLTLYMTNTKGEEILYFEKKSGVLGIKMEVYGSKENFLGAI
                     QKKAHSIRHTYEVQNPAGQTVYLIEGPAMMPEVFTITKQEVKVGRMSRKMTKQFEEGV
                     SRQDHFSLIFPMGIDSQDKGVLLGGLILIDLSH"
     gene            19343..>20305
                     /locus_tag="PK590_00550"
     CDS             19343..>20305
                     /locus_tag="PK590_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014955863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOG23118.1"
                     /translation="MLEEKKIPRAWYNLAADLPNPAQPPLGPDGQPISPQMLGKVFPM
                     NLIEQEMSRERWIKIPEEVLGILYRWRPTPLRRAVFLEQYLKTPARIYYKDESVSPAG
                     SHKPNTAVAQAWYNKEFGIKKLSTETGAGQWGCALAFACKLLDLECKVFMVRISFDQK
                     PLRKMMMKVWGADCVASPSPETNAGREILKETPDTPGSLGIAISEAVERAVMDPSGET
                     RYALGSVLNHVMLHQTIIGLEAKEQLKIAGEKKVDVVIGCAGGGSNFAGISFPFVCDK
                     IHGEKITILPVEPTACPTLTRGPFAYDHGDVAKMTPLLAMHSLGH"
BASE COUNT         4771 a         4903 c         5493 g         5138 t
ORIGIN      
        1 cctggctgga tgacacgcgc gacacgatgg tctggcgctt tgaccgccgc gatctggagc
       61 acgccgcgga gcttgttaac cacgagggtg gccaaagctt caccttaatg tcctcagata
      121 caatgaggag cggttttccg agccgcgcca ctttctcaag caccgggacc agatctttga
      181 cggctgaaat tttcttctcg taaagaagaa tgtagggctc tttcaggacc acttccattc
      241 gctccgcgtc cgtcacaaaa taaggggagg aatagccgcg atcaaactgc ataccttcca
      301 ccacctcaag ttcggttttc atccccttcg cttcctcgac cgtgatgacc ccgtccttgc
      361 cgaccttttc aagcgcgtcc gcgaggagtt taccgatcgt cggatcgttg ttcgcgctga
      421 tggtgccaac ggccgtaatg tcctctttgc ccttaatagg tttggaaagt tttttgatgg
      481 actcgatgat tttcgcggcg gctttttcaa tcccgcgttt caaggccatc ggattggctc
      541 cggcggtgac gttcttcaga ccttcagtga cgatcgcctg ggcgagcacc gttgcggtgg
      601 ttgttccgtc gccagcgagg tcagaagtct tttccgcgac ctcacggacc atctggcaac
      661 ccatattttc gtaagggtcc ttcaggtcga tttctttcgc gacggtgacg ccgtctttgg
      721 tgataagcgg cgcgccgaac ttgcggtcaa tcacgacatt tctgccgcga ggccccagcg
      781 tgacagcgac cgcgcgggtc agaacatcga tgccgcgttt gatcttttgt cgcgcttctt
      841 cagaataggc caacaatttt gcgctcataa tgttgctcct ttgtttttaa aaatttgaaa
      901 catgaaacgc gaagtttgaa atccattttg ggggatggat accaacctcg ctgtttcagt
      961 tttcgaaact tacttattgg tgtcgtaaac cgccagaatc tcactttctt tcaggatttt
     1021 gtgcttcgtt ccgtcgatca cgatgtcgtc gccggagtat ttcccgaaaa gaacgcggtc
     1081 gcccttcttg acctcgatag gctgaacctt tccggactcg agcatttttc cggtaccgac
     1141 cgcgatgact tttccttcag tcttttcttc cttggcggta tccggaagga taatgccgcc
     1201 tttggttttt tcttcagctt caagaatttc cacgagaact ctgtctgcta acggtctgac
     1261 tttcatggtt ggatctccta tgatttttta gataacagtt tttgttagtg cgatacgcgg
     1321 gatatgttga cctagtagtc cattcccccc attcccccca taccgcccat gccacccata
     1381 cctcccattc ctccacctcc cggcatcgga ggcattttct cctcttcggg aatgtcggtg
     1441 atcagggttt cggtcgtcaa gagaagcccg gcgacactgg acgcgttttg gagcgcgcta
     1501 cgagtgacct ttttcgggtc gatgatacca gcctgaacga ggtcctcaaa ctcaccggtt
     1561 tcggcgttat agcccacgtt gcctttttca gacatgaccg cctggacgac aaccgaacct
     1621 tcgtcgcccg cgttttgcgc gagctggcgg agcggctcct cgagcgcccg gcgaatgatg
     1681 tcagcgccga ccgcctcatc tcccttgagc ttcaggttgt ccaggacgga tttcgcccga
     1741 agcaacgcga cgcctccgcc agggacgatg ccttcttcca cggccgcccg ggtcgcgtgc
     1801 atggcgtctt ccacgcgagc tttcttttcc ttcatttccg tttcggtcgc ggccccgaca
     1861 tggatgactg ccacaccgcc cgcgagtttc gcgagccgtt cctggagttt ttccttgtcg
     1921 tagtcggaat ccgtttcttc gatctgtttc cggatctggt tgatacggcc cgtaatgtcc
     1981 gcgctcttgc ccgcgccttc aatcagggtg gtgttttcct tgtccaccgt gacgcgtttc
     2041 gcgcggccga gatccttcag cgtaacgttc tcgagcttga tgccgaggtc ctcggtaatc
     2101 gccttgccgc ccgtcaggat cgcgatatct tcaagcatcg ctttgcggcg gtcgccataa
     2161 ccgggcgctt tgacggcaca aaccgtgagc gtcccgcgga ttttgttgac gacgagcgtc
     2221 gcgagcgctt caccctcgac gtcttccgcg atgatgagaa ggggtttccc ggctttggac
     2281 gccgcttcca ggaccggaag catgtctttt accgcggaaa ttttcttctc attaataaga
     2341 atgtaaggtt cttcgagcac gacttccatg cgctcggcgt ccgtgacgaa gtaaggggag
     2401 agataaccct ggtcgaactg catcccttcg accacgtcaa gggttgttgc ggtcgtcttg
     2461 gcttcctcga ccgtgatcac cccatcttta ccgaccttgt ccatcgcttc ggcaatttgc
     2521 tcgccgatgg tcgtgtccga attcgccgcg atcgtggcga tctgcgcgat ttcttttttg
     2581 tccctgccga cgggttttga aattttattg agttcctcaa cgacggcttc gacggctttg
     2641 tcgatcccgc gtttcagggc catcgggttg gctccggcag taacgttttt caacccttcc
     2701 cggcagatgg cttgcgcgag aatggttgcc gtagtggttc cgtcgccggc aatgtcggaa
     2761 gtctttgaag cgacttccct gaccatttgg gcgcccatat tttcataagg gtcttccagc
     2821 tcaatttcct tggctactga cacgccatcc ttggtgacgg tcggggaacc atatttcttt
     2881 tcaaggacga cattacggcc cttgggaccc aaagtcactt taacggcatt ggccagtttg
     2941 tcgacgccgg cgaggacagc ccgccgcgcc tcctcactga atagaagttg ctttgccata
     3001 ttgtgttacc tccgttgtta gtcgccatcc cgatcaaagc gaaggatctt gtacctggga
     3061 ttcttctgct gttggccccg gaatgacgaa ttctgatcaa ttagaagatt caatcgaggt
     3121 gttggcactc atgatcgctg agtgctaaca tagcgttatt ctatggatta acaaaaacag
     3181 gtcaagagga aaaattcagt gcgaaaaact tatttgaaaa gagaatgatt tttctatgga
     3241 aactgttgaa ttttttctcg ccagatgagc gcgagtgatt tgagggtatg gaaagggtcc
     3301 agaatatcga tcgatgcaga atgaggggcg attgttttag atagaccgcc cgtcgcgagg
     3361 acccggattt ttttatcgta gcgaattttg aaccggcggg cgagctcgtc ggccatggat
     3421 ccatatccct ctaaaatgcc gtattccata catttttttg tgtctcgtcc caaaaaacca
     3481 ggcttttttc tagggaagtg gatggttgga agcaaagagg cgctttttga caaggctttc
     3541 agagcgattg ctggccccgg aataataagc cctccctgga aagttccggt tcggtccagg
     3601 tagtcgcagg ttatcgctgt gccatagctt atgataagga gtggcatttt atacatcttt
     3661 aaagccccat agcaaccgac cttcctgtcc attcctaact tattgtattc tttatagtta
     3721 tgtctgattt tcaggtcaat atttgaccct aaaacccata attttgcatt attatgacac
     3781 accattgatt ttttaatata agatgtcttt tttgggtcaa cggagcatat taagacgtca
     3841 agatgattat atttaccact ttttatgaat ttattgatat attttgggac gtcattaatt
     3901 tttgacgttt tacagttaaa gatggcgccc gatttgcaga gacctgtctt gacagctgtg
     3961 ttcccgatat caatggcgag aaggaaatta cccatgattt tccccataga aaatgataag
     4021 tctttctttg agtgccccaa gaaggttttc caggacattc cggatttcat agtttcgcgt
     4081 cttgagaatt cgcattgacg ttgctcccga gggcagctca ttgatttccg cgttcacatt
     4141 aaggcctatt cccattacga cggcttcaag atgaggcccc gtcgtaattg attcggtcag
     4201 aataccacaa atctttttac catccgacat gacgtcattc gggcgtttgc aggttgaggg
     4261 gattccataa tgggtttcca atgtctgggc cactgcctga catccgatct gggtcagcat
     4321 gggggccttc gcgggtgtta tcggagggcg caagaggatg gaagcgagca gatttttcct
     4381 ctccggcgat tcccacgtgc gtcccggttt cccgcggccg gcagtttgat gttccgcgac
     4441 gatgacaaga ccctcaggtt cacccttccg ggcgcgctcc agggcaagcg tgttggtcga
     4501 tgttgcctcc cgcaagtgtt ccacgcgaaa tgaaatcaat ggggcacctc tcaagaccct
     4561 ttcaaaatga gccgccagaa tccggtttca gaattcaggc aacaactctc ggaagatttg
     4621 cgtttagttg ggtcacgatc tcatagggaa tcgatccggc ccattgggcc acgtcttccg
     4681 ctgaaatgcg ctcatcctgg tctttgccga tcaaggtgac gatgtcgcct tccgcggctt
     4741 cctgtcttcc gagattaact gttaaatagt ccatggagat ttttccggca aggggataac
     4801 ggttccctct ataaaggacc tgcgcgtttt tccatgcgct ccagggatat ccgtgactat
     4861 agccgagagg aaggatcgcg aggttcgttg ttgatgatgc cgtaaaggtg cgtccgtatc
     4921 ccgcggtttc gccgctttcg attcttttaa tgaatgtgat cctgctttta agctcgagga
     4981 cgggggccag cttgacggtg tcgcgtagcg aggggtcagg gtgaattccg tagagagcga
     5041 ggcccggtcg aacaatattt aaaagagaat tttttagggt aagactggcc gcgctgtttt
     5101 gcgcgtggcg aaaccggaat gcgatgccgt ttcgatccag ttcctcaaga agggattgaa
     5161 atttctggat ctgctttgtc cggaagaggt cttccacctc ggcggtcggg aaatgcatgt
     5221 aaatgccgtc aagtaacagt ccctggagga gccggatttt catgatctcc cggacggcct
     5281 cgtcggctct gaatcccatg cgccccatcc ccgtgtcgat tttgagatga acggaagctt
     5341 tccgttccag agattccgcg caatcagaaa tagctcgggc ctgggcgaaa cttgagacgc
     5401 tcatagttat gttagagccg accgcgaccg ggatctcgtg gggcagaaga tgtccgaaca
     5461 ggaaaacaga agtttttgga aagtgttcct taagcgacag tccttccttt aaagttgcca
     5521 caccgaaaaa cttgaccttg ccttccagga tctgcgcgat ttccctcaat ccgtggccgt
     5581 acgcgttggc tttgatcacg gcgagaacgt ccggagttcc ggaacgcgcg cgtatggttt
     5641 ccaggttcgc gaggaggtga ttcctgctca gctcgatcca cgaggcgtat tgatgtttga
     5701 ccgttgtgct accggcggtg ttttgcataa aaaagcccgg ctttcaaatt tcgaagaatt
     5761 tatttttgat tttgtgtttt attttttaat cgttcctctg cctccgagtt tctcagataa
     5821 aaaggcagaa aatccttcgg ccttttaaat ggcttgagca gaggatcctt tttttcatag
     5881 agattaatca gcgcggacgc cctaggcgtc cagtacttct cgggaagcga atggatcttt
     5941 tttaaaagat cccgatcgca aacagctaac ccgtctcccg cgacccgcgt gccggcagga
     6001 agttctttta aaaaatcatc gggagctttc acaaaaggcg ctcccgccgg tttccagcct
     6061 tgtctataag aacggtacaa acgggcgtaa agtttatcac gctttgcgtt cagacacacg
     6121 gccagatttt ctccgctcgc gcagcgagat ccggagcggg gaatggtcga agcgacaagg
     6181 tcgaaggagt aggccccgaa gcaaggtctt tcacgaacgg caagaaaacc tttcaaggtg
     6241 gcaaatccga cgcgcaaccc agtgaatgag ccggggcccc gcccgatcag gaaggtgtcc
     6301 acctctttta ttttgaggcg ttcttttttc agaagccggt ctatcaacgg gatgagattt
     6361 tcaacatggc gtgaaaaacc tttgagaaag gcgtgacggg cgggagcgcg acctttttta
     6421 attgcgacgc tcagggaatg tccggaagtt tcaatcgcca gtaaattcat gataaagcgc
     6481 gtgccgtctg ggttatgtat gtttttttat aagcggggag ctatttcctc cgaaaatttt
     6541 gctcctttca ggagcgtaaa ctccggcgga agtcgtcctg atcggcattc tattctgaaa
     6601 cgggaaaaag agaaacggga attacaaaag acgtcagaga acaaagaggg aaaggtgctt
     6661 gaccggggct gaaaaagccc cgcctttttc tgaaaatctg ccatttgttc aggagagccc
     6721 cgattgagtt cctgccattt tctggtagga tatccatccc ttcacactcc gctgaacgta
     6781 aggatccggg aaatgatctc aacgaacgga attacattac aatacgggaa acgcattctt
     6841 tttgaggatg tgaatatcat gttcacgccc ggcaactgtt acgggttgat cggggccaac
     6901 ggttccggga agtccacgtt tcttaaaatc ctcgcgggat tggtcgagcc gaatgccgga
     6961 accgtgacgg ccggaaaaaa tgagcgcatc tcttttctca ggcaggacca gtttgctttt
     7021 gacgaattta cggcgctgtc gaccgtgatc atgggccacg aacggcttta caatatcatc
     7081 agggaaaaag acaacctcta cgcgaaatcc gatttttcgg aaaaggatgg tctgcgggtc
     7141 gcggaactgg aacatgaatt ttcggaaatg aacgggtggg acgccgaacc tgaggcggct
     7201 aaacttctga gcgatctcgg aattccggat gcccggcacc aggtccccat gaaacaactg
     7261 gaatcgaacg aaaaggtccg cgtgcttctc gcgcaggcgc ttttcgggaa tcccgatgtc
     7321 cttttgcttg acgagcccac gaaccatctg gacgttgaga cctgcctgtg gcttgaggat
     7381 tttctctacg aatttaagaa taccgcgatc gtggtttcgc atgaccgcca tttcctcgat
     7441 cgcgtttgta cccatatcgc ggacattgat tacggcaaga tccagcttta cgcggggaat
     7501 ttctcgtttt gggtagaatc cagtcaactt gcgctccggc agagaaccga gaccaacaag
     7561 gatatcgcgg ctcgccgcaa ggaactccag gagtttatcg cgcggttcag cgcgaacgcc
     7621 tcgaaatcac gccaggcgac atcccggaag aagcttcttg aaaaactgac gctggaggac
     7681 atcaaaccgt cctcccgaaa atacccttat atcgcgttta aggcggaacg cgactccgga
     7741 aatgatctcc ttgagatcga ggggcttgaa aaatcgatcg aaggggagat gatgttcacg
     7801 ggacttgatc ttcaagtgag caagggggac aaaatcgcgt tcctcgggcc gcttgaccgg
     7861 gtcaagacca cgctgtttca agtgcttgtg ggcgagatca aggcggatcg gggatccttt
     7921 aaatggggag cttccaccac gcgagcttac tatccgaagg agaacggaaa atattttgaa
     7981 acggacctga acttggtcga ctggttacgg cagttttcga aagaaacgga tgaaaatttt
     8041 attcgcggtt ttttgggaag gatgcttttt tccggggaag aatccctgaa aaaggcttcg
     8101 gtgctctcgg gcggggaacg ggtacggtgt atgctttctc gcatgatgct cgtcggcgcg
     8161 aatgtcctaa tcctggacga acccaccaac catcttgacc tggagtctat tacgtcgctc
     8221 aataaaggtc ttgagaactt caaggggacg gtgctttttt cctctcacga ccatcaattt
     8281 attcagacgg tcgcgaaccg gatcgttgag ttgacgccgc gggggattat tgatcgccgg
     8341 gacatgacgc ttgatgatta ccttgctgat cccgggctca aggttcagcg cgccgctctt
     8401 tacgctcccg cgtgattttt ttttgacccg aggtcgaaaa aaacgatagc gtgatttaaa
     8461 aaaagagcag ttttacttta tcagcatttg aaagatccgc tacgtggcgt ggccccggaa
     8521 tcaattttaa agtggttggt cggagatttc ggttgggagg ggccggtgga gcgcgtctgt
     8581 tccacgatgc tcgcgatttc ttccgcttct tttttctttc ctcgttttaa tgccccctgg
     8641 atgaaaattc gggcttttcc ctccagggtt tttcttgcgg ccatggtttg ttccggtgtc
     8701 aggatgcctt ccttcagtat tttcgcgagt gaacggattc gaaaccgatc catggccggg
     8761 aattcgcggg agcgctggcc cgcgtggccg ccatattttt taatataagg tttgtcgatc
     8821 agaccgatcg gataccgtga ggcgatcctg agccagaggt cgtagtcctc gcaggcggga
     8881 agggattcat cgaaaaatcc aacctcgttg aaaatcgtcc ggtggatcat gacggcggag
     8941 ggactgacga tacaaagagg caggcatcgt tcgaagatcc agccgccgta ttttttgtgt
     9001 tttttcatgg ggttaacccg ctgaccgttc cggatccaga tctcttcggt ttgaccaatc
     9061 cggaaatttg gattctgttc aaagaaatcc aactgggccc ggagttttcc ggggagccat
     9121 tcatcatctg agtcgaggaa cgcgatccat tctccccggg attccttgat cccgaagttt
     9181 cgcgcggccg aaggaccctg gtttgtctga aaaaggtagc gaatttgcac ggcggaagga
     9241 tggcgatgcg cggagttttt gtccggagtc ttttcgccgc gcgccatgcg ttgtatgtct
     9301 cgctcatttg ttccgtcggc ggatccgtca tccacgataa tcagctcaaa acgcgcgcct
     9361 ttttgcgcga gcacagaccg aatcgcgcgc gataacatcg cggaacggtt gtaggttgga
     9421 atgatgacgc tagtctggat catggcgcgg gattatagca aaagggcgat gaaggaaaaa
     9481 tttttttacg ttccccgttc cgtggagaat ttctttgtgt tatgatgtca aaacttatga
     9541 cggcccagaa aaaaacaacg aaagacaatc ctaggaattc tgtcacgaaa acagaccttt
     9601 cccggaaact taaaattctc tttatcgcgt ccgaggcagt tccctttgtc aagaccggcg
     9661 ggttggggga tgtctgcggg acgctcccga aggcgctaaa aaaactcggg cacgatgtcc
     9721 ggctcgtgct cccccgatac tgggccgttg atcgctatgc ctggaagctc acgacggcgc
     9781 ttgccccgat gggggtagaa atgggggacc ggactatttg gtgtgaggcg ctcaaaggga
     9841 aggctgacgg atttaccgtt tatttcatcg aacacgagca gtattttggc cgggccggac
     9901 tatatgatga tggcaattgg gaacatccgg acaaccttga acggtttggt tttttctcga
     9961 aagcggcgct tcagcttgcc aaagacatca aatttcgccc tgatattgtt cacgcgcatg
    10021 actggcaggc cgccctcgct cccgcgtacc tgaagacggt gtacaaaaac gacccctttt
    10081 tcaaaaagac ggcaagtgtt ttttccatcc ataacattgc ttaccaggga acgtttaagt
    10141 cggatcaata cgggtttctc gggctcgggg cggaaaattt ccggcaagaa gtttttgaaa
    10201 actatcaggg gatcaatctg atgaaaggcg ctattttttt tgcggacgga atttcgacgg
    10261 tgagtccgac ctatgcccgc gaaatccttt ccgagcctgg aggcaacggt ctttccgatt
    10321 atttaaatcg acgtcgggaa gacatctcgg gagttttgaa cggcgccgac tatgatcatt
    10381 gggatcccgc cgtcgacaaa cttcttccgg cgcgctacac ggctgatgat ttgcacggga
    10441 agacaatctg caaacgcgtc ctccaggaag agtttcagct tgatattgag ccgaaggctc
    10501 ccgtgatcgg cgttatttca cggtttaccc agcagaaagg ttttcagaat ctgatgcccg
    10561 tggtggccaa tctttttcag gaaactccgg cccaaatggc gattctcgga acgggtgaaa
    10621 aatggcagga agattttttc aggggactgc cggccgctta tcgcgggcgg gcgggagcct
    10681 ggatcggtta cagcaacagg aaagctcacc tgatcgaagc cggcgccgat tttttactgg
    10741 tgccgtctct ttatgaacct tgtggcctaa accagatgta cagcctccgc tacgggacgc
    10801 tcccgattgt tcgagcgacc ggagggttaa aagacacggt ggttcagtat gaggaatcca
    10861 cgggagcggg cacgggattt cttttttacg acgcgactcc tggagcgatt ttggaaaccg
    10921 tgaagtgggc ggtcaggacc tatctggatc ggccgaaaca cattgaaata ttgcggcggc
    10981 gggcgatgcg acagtccttt ccctggtcgg attccgcgaa acattatgaa gcaatctacc
    11041 aacgcgcccg gaaacgtcgg ctcatgtggc tatgagcgac gcgctataaa aagatttaaa
    11101 acattccctt gaattttagt gattttcccc gtggcctatt gcgatggcat agttttttga
    11161 aatgaacagg actattttaa aagggcccgt gtctcagaag gcatgggaat ccgcaggaat
    11221 gttgaaatca tgttatcctt ttcccgtgat ctctgtagta taatcactcc cagttttcag
    11281 gaggtttttc atgtcccagt ccactaaagc ttacgacaaa ctcagccttt cggaatatct
    11341 tgccaagctt tcttcagatt cgccggtgcc cggcggcggt agtgtctccg cttatgtggc
    11401 gtcactcggg atggggctca cgcagatggt ggcgcggatc gcgctgaatc gaaaaccgaa
    11461 agctggggcg acgccggaac aaaaatttca gcatgaggcc aaccggaaga gaatggaaga
    11521 aattatttct tcccttgaaa aggccaaggc ccaagcgctt caggtggttt cgcttgatcc
    11581 ccaggtgtat gacgaggtga tggcttgtta ccgggagaat gcccagccgg aaaaacttga
    11641 agatgctctc gagcacgcgt ttcgccttca ggcggatctt gcgttgatca tcgagatggc
    11701 gcgggagtgg aatactcaca tgaaaggact tgtccaggga gctatcgcga acgatctcgt
    11761 ggtaagcgag aggttgcttg cggcggcctt tgaaggcgct taccataccg cgcgtatcaa
    11821 tgtggtttat atgaaaaatc aagaacgtaa agcgcgggcc gagcaggcgc ttgaagaact
    11881 gaacaggcgc tatcagcaag gaagggaatc ttgaaaatgg aatcaaaaag actcgaagga
    11941 aaactgattg ccgccgaaat ccaaacaagg attgaaaaaa cagttcggga aattctcacc
    12001 cggggcgctt ctcgaccaag acttgtttcc attcaaacca gtgaggatcc ctccgcggtc
    12061 tggtatgttg gacagcaaga aaagctcgcg gcgaaacttg gcattgattt ccaaagaatt
    12121 tccccgcaag aggttttaac cgagaaggct ctgatcgaaa aagttcaaag ccttagcgcc
    12181 gacccaaaag ttcacggtct ttttatttcc atgcctttac cggaaggatt tgacgcggac
    12241 aaggtgctcc tcgcgatgga ttcgcgtaag gatgttgaag gcatccatcc cgcgagcctg
    12301 ggactgatca tgctccggaa aggcaaattg attcccccga cggcctacgc ggcattcagt
    12361 cttattaaat caaccgaaat tatgttgcgc gggaaaaaag cggtgatcat agggcagagc
    12421 gcgattgtgg gaagaccgct ccagctcttg ctgggggaag cgcgagtgac aacacttgtg
    12481 tgcaataccg ggacgtccga ggaagacatc aaaaaactgg ttggcatggc ggatattgtc
    12541 atcggatgcg cggggaaacc cgggatgatc aaaggcgagt ggatcaaaaa gggcgccgtc
    12601 gtgattgatg ttggaaccac ggaggttgag gggaaactgg tgggagatat tgagtttgag
    12661 gccgcttccc tgaaagccgc ttttatcaca ccagttcccg gaggcgtagg ccctctgacg
    12721 gtaacgatgc tcatgaaaaa tttgataaca gcgtatgaat ggcaaaacaa atgacgcgtg
    12781 acacaaagtc gcgaggacgt aaaacatggc gcggattttt ttcgtggcct gtggtttagg
    12841 atatgtgacg tgatatgaaa cttacacctg atgatattta ttcaaagaaa gcaagaggcg
    12901 agaaaatcac ggcgttgacc gcgtacgatt ttccaacggc acgcatcctt gatggagccg
    12961 gcgtggacgt tttggtgatc ggggattctc tcgggatggt tcttctcggg gatgagacga
    13021 ctcatcaggt gacggtttca gatgtcgctt atcataccaa ggccgttgcc aaagccgccc
    13081 aacgcgctct cgttgtcgct gatatgcctt accggtcgta cctctctccc gaagaagctc
    13141 tttcaaacgc gcggattctt gtcagggatt ccagcgctaa tgcggtcaag atcgagggag
    13201 gggtagccat tgaagagcag attcgtcatc tggtcaggaa cgggattccc gttgtggggc
    13261 acatcggtgt gcttcctcaa agcgttgaag gcaagaaata cagggttttc gggaaaagag
    13321 aaccggaagc cgaatctatc atgaaagacg ccaaacttct ggagagtttg ggagtttttg
    13381 cgatagtcct ggaatgtgtc cccgggacgc ttgcggcgcg agtaacggcc gaggtgaaat
    13441 gtccgacgat cgggattggg gcgggagctt ccaccgacgg tcaaatcctc gtggtgcatg
    13501 atatcctcgg aatccggagt tccgtgagcc cgagatttgt gcgccggtac gccgatttgg
    13561 aaaatgtgat aaaacaggcg gctgaaggat attgccgcga cgtggcggcg ggaacatttc
    13621 cttctcaaaa agaaagtttc tgagtatgag caagggcaag aaaattcttg tcatcgaaaa
    13681 ggaggaagag gcgcgtgagt tgatggtcat gcgcctggaa tcccgtcacc acaaagtgat
    13741 ccaggcttct tccactccgc tggcgatgcg cgcccttgag cgcgaagtgc tcgatctgat
    13801 cttgctttcg acggatatgg aaccggtgga cgggaaaccc ctgctacagg tgatccgttc
    13861 caggtcccat ttgatggccg ttccgatcat cctgctggcg aaggaaagca atctgacgga
    13921 actcatgctc ggccacgaaa aaggatacga tgattttatc atcaagcccg tcaaccccct
    13981 tgttttgcaa atccgggtta atctgagtat ttcaaaaacc caggagcgtg tcgaagcgaa
    14041 cgccctgacg caccttcccg ggaatcatgc gatcgaaaaa attgtttcca agaaaatcag
    14101 ggcgggagaa aagttttcag ttctttatat tgatgtcaat cacttcaagt cctttaatga
    14161 ccgttatgga ttccagaaag gcgacgatgt cattcttcaa acggcgcggc tcttggtagc
    14221 gaccagttcc gcggtatctt ccgcgggaga atgttttgtc ggtcatctcg gaggagatga
    14281 ttttattgtg gtgacccctc cggaactcga ggatgttttt gccagacagt ttctggatga
    14341 ttttgatagt gtgatgccga cctattacag taaggaagat cgggaggccg gttgcattcg
    14401 cgttgagaac cgtcgcggcg aaatggaaaa ttttccgctt atgtcctgct cggtcgcggc
    14461 ctgcaccaat ctttacaaaa attacacatc gcttcgggaa atttcccagg acgccgtgga
    14521 ggtgaaatct tttttgaaga cacagcccgg gagccattac ctccaggatc gccgctccat
    14581 gcccattcaa aaaattgatg aagcggttca tatcctcgcg cccgaaatca aagcctcggc
    14641 cagacgccac aaaaaatcca gggtggatcc attgggccaa gtgctcctca gcgccggtct
    14701 gattaacgaa agccagcttt cagaagcgct gaaaaaacat tttgaaaccg ggcagaggtt
    14761 gggacaaaca ctgattggca tgaaccttgt tccaagccat gaagtcggaa agatgctgga
    14821 acagaaactg aaactgcctt attttagact gaaggactgg tctccctcgc cggaacttcg
    14881 ggagcttttt accggagatc tcattgcccg ccaccgggtg atgcctgttg aagtgaccga
    14941 gggtgccgtc aagctcgcga tgtgcgaccc tttcgatatc cggactctcg atatgattga
    15001 gcgtattacc ggactgaagc cgatcccctg cctcgcgctt gaagacgagt ttgaggtctt
    15061 tctcgaaaaa aactttccct cccaaccggt tgacatcaat taatccgcgg gatttaaacc
    15121 gggatcttca gtcctcaaaa cgcgtcgaat gagaaaggat tgaaaatccg cgaacattca
    15181 gcggatgttt gcttcaaaaa caaagtgaag attcttcccg ttatggtcgt ttctttgtac
    15241 agaaaccgga tggaagttgc cccctgtttc agaaaactat gggaatagaa tcttttccag
    15301 agatagaaaa ttttcaaaag ttgccccgtg aggtgatcat caagggagcg agcaccaaat
    15361 tcgggcgtga acatggcgcc gagttgcgag ggattgtcat caacaacatc ggccagccca
    15421 tcagaaacat tcgcgcgcag ctcgtggtgt tcggaaaaaa taaagtgccg gttctcggga
    15481 ccagttcggc cgcggacccc gacaaattgc cgcaaggggg gatcgcaaag tttcgatttt
    15541 tccttaaggg atttaaacag gatctcaaga attatcatct tcgggttgtc tggaactttg
    15601 atgaatcgtc gtgaaggcgt ttgaggcgtc ttctccgaaa tatcatgcgc gttaaagaag
    15661 aagttatttt ttggtaccaa atgccgggca gaggcgtgaa aggtcttacc gattcaacgc
    15721 tggaggatag ggtggtgaaa ttggcccgga tttttcacgg ttaaccggga aagagaggag
    15781 aacaatgaag acaactacag aaatagaaat gaaggcaatc aaatcccgcg atatcgcgct
    15841 tgttggctac gacgcggggg cggagatcct tgaagtggtt tttcgagcgg ggggagtcta
    15901 tcggtatcag aacgtccccg aatacgttta tcaggaactg atctcggctt cgtcgcctgg
    15961 gacgtatttt caacactacg tgaaaaacac cttctcctac gtgaaagtgc ggtaattatt
    16021 accgaagttt atggtcctgc gtatcaaaac tttgctggtg gtgttttgcc tcttgttccc
    16081 ggcgagcggt tgggttcacg cccgtttgcc gattcgggag agtgaggctt ttcaacaata
    16141 tctcaagaaa ccccgcggag aattcgccaa acttgtttgt cttctgaatt attaccggga
    16201 cggcgatttt atcattcgtt ttgacggttt tgattacgtg ccctccttcg cttttcctca
    16261 cgcgaaagcg tggcttttca ctcattacaa caaggacacg gcctcgcagt ggatccggaa
    16321 aaactgctat gtttctcctt ttgagcggaa ggttatttac gttaaatttc cggatgggga
    16381 gcttgagccg gcgcgggata tgatcctccg cgacctggcc gaactggagc gggcgatagc
    16441 ggaagttcat gcggtctgaa atcccgcgct ctgaaaacat gattcctgaa acgcgtagca
    16501 cagttaaaat ttttctcctg acggttttcg tcatctgttt ttggggggcg gaggtacagg
    16561 cggaaactcc tttcaaggta acggtgaagc ccgaccagag ctccgtgcag ccgggaatga
    16621 tgtttcttgt caatgcgggc gtaaccaatc ggaccgaaaa cactgtaatt cttcaggtgc
    16681 cttcctgcgc gtttgacaaa ctttgggaag tggacggcga acatatccgg attcagccct
    16741 ggacctgtca ccagaacacg accgaaagag cggagatttc ccccggggaa agctatgaga
    16801 aaaatcttat tctgtatgcc tcggacgtga atcagccggg accggtgacg tttcggatgg
    16861 gttttaaaca tgtgttggaa aacggggatg aaatggagcc ggtctggagt gatcccgtca
    16921 cgatccagat tttgataccg gaggggccgt tgaaggaatc ggctctaccc ggcgaggagg
    16981 cgagaccgat ctcccaaagt ccggcgggcg ttgaaatatc cgaggtgatg cccgatgaaa
    17041 tttcggaaga tgaggttttc ggtattgagg gagaatcccc gacgtcccag catcgcgatg
    17101 agtcttttaa tgtcccggac atggaccccg cccaaagcgg tgtataaaaa gccggtcttt
    17161 tgaaatgatc ccgaaggttg tgatccttgg aggcggtatt tccggtcttg taaccgcgca
    17221 tgagcttgcc cgttgctccc cggcccaaat cactctcgtt gaatcctcga cgcgtctggg
    17281 cggagtaatt cgaaccttga gaccggagtt ttttgagatg gaagccgccg ttgacacgct
    17341 tgacggaaaa aacggaagcg ttcttgaact ttgtaaggat ctggaacttg agagttatct
    17401 tgtcccccgc gaatctgctc tcgcgcattt ttttcttaag agaaacagag aaatttttcc
    17461 cgtcggtttt tctcccgaaa tttttaaaaa caacgcttta accctggcca ccaaaatgcg
    17521 aatgctggga gaggtgatga tcccgcgacg aaaggacgcg atggatgaaa gcgtgggaag
    17581 ttttattcgc cgccggttcg gaaatctttt ttttacgcaa atagtccagc ccgtgatccg
    17641 ggggggcatg atggccgatc cggacgatct gggtgtcggg gaatatttta aacggtgggt
    17701 tgaggcggaa tcacgtttcg gcagtttgat ccgtgccgtt ttatccggaa agtttcccgg
    17761 aacggccagg atgacggcct tttcggttcg aggtggtatg gatcgactgg tagcgggttt
    17821 tttaaaacga ctcccggaga tccatgttct tttaaattca agggcggtta ctgtccgaaa
    17881 ggatcagtcc tggatagccg aattggagga cggaacccgg cttagtgcgg gcgctatttg
    17941 tcttgccttg ccagctcacg aatcgtcgcg ggtgcttgat aaaaatttct cagacctttc
    18001 ccgtgatctt gaaaaaatca attacgaaac ggtggcggtt atcaatctgg tatttcccgc
    18061 ccaagggttc ccgcgtgaat tttcaaagac cggatttttt attcccgaga acgggaggga
    18121 aagccctttt tcttccgtca aattttgtgg tcctgaagag accggagcgt ggattcggat
    18181 gaaggttttt attgcggaaa aatttcatcg tgaagtgttc ttgctggagg acaaggctct
    18241 tttggaaaaa gtggcgcgaa atcttgagga gcaatgggga ttcccggaac ctttttgggc
    18301 ccaggttgaa cgttactgga aggcgatcgc gcaatatcct cccgggcatc ggaatcttgt
    18361 tcgcgaagtt gattctcgcg ctcaaagaca tccggggctt tttcttgccg gtaacggata
    18421 tcgtggtttt ggtgtgccgg attgcatcga gcgagcccgg gaaacggctc gaaggatcgc
    18481 ggattttttg aatcggaaat gatgttcccc taaaagcggc gaattcgagg cgatatggag
    18541 aaaacggttc caaacattta tggaggcggg aagataaatc gggtaaaata ccactgaaaa
    18601 ttacgtttta aaatccatct tttaaagtcg cgagattccg caaggcaagg gacgtttatg
    18661 gatgttctga aatcaatcga ttctctggta atccgggaaa aaaaggaata tacgggtgtt
    18721 ccatcaacat ggaaagtaag acctgaattt tgcttatccg attcttccga aaaggaatgt
    18781 ttctatcttt tttccgaaaa ggggactgcc cagggtttcc cggcgcttca ggaaagcaag
    18841 tcactgacgc tttacatgac gaataccaag ggcgaagaaa ttctttattt tgagaaaaag
    18901 tcgggagttc tcggaataaa gatggaagtg tacgggagca aagaaaattt tttgggagcc
    18961 attcagaaaa aggctcactc catccgtcat acttatgaag ttcaaaatcc cgcgggacaa
    19021 accgtctacc tgattgaggg gcccgcgatg atgccggaag tttttacgat tacaaaacag
    19081 gaagttaaag tcggccgcat gagcagaaaa atgacgaagc agtttgaaga aggcgtttcc
    19141 cggcaggacc attttagcct gattttcccc atgggaattg attcccagga caagggtgtt
    19201 ttgctggggg gtctgatcct gattgatttg tcgcattaaa catcctgcgg tttgtgagcc
    19261 ggaacgactt tgggataaaa agaacaagat taaaaaataa aaatattttg agaggagcgc
    19321 tgaattgaaa cctgtaaaaa tcatgcttga ggaaaagaaa ataccacgcg cgtggtacaa
    19381 cctggcggct gatcttccca accccgccca accgccgctt ggtccggacg gccaacctat
    19441 ttctccccag atgcttggga aagtgttccc tatgaatctc attgagcagg aaatgagccg
    19501 ggagagatgg attaaaattc cggaggaagt tctcggcatt ctttaccgct ggcgtccgac
    19561 cccactgagg cgcgcggtgt ttctcgagca gtatctcaaa actccggcca ggatttacta
    19621 caaggatgaa agcgtcagtc cggcggggag tcacaagcct aatacggcgg tggcccaggc
    19681 ctggtataac aaagagttcg ggattaaaaa actgtcgacc gaaaccggcg cgggccagtg
    19741 gggatgcgcg cttgcttttg cctgtaagct tcttgatctt gagtgcaagg tttttatggt
    19801 ccggatcagt ttcgatcaga aacctctgcg aaaaatgatg atgaaggttt ggggcgcgga
    19861 ttgcgtcgcg agtcccagtc ccgaaacaaa tgccgggaga gaaattttaa aggaaacgcc
    19921 tgatacgcca ggaagcctcg gcatcgcgat cagtgaagcg gtggaaaggg cggtgatgga
    19981 tccgagcggg gagacccgtt acgcgctcgg gagcgtgctt aatcacgtga tgctccatca
    20041 gacgataatc gggcttgaag ctaaagaaca gctcaagatc gccggggaaa agaaagtgga
    20101 tgtggtgatc gggtgcgccg gcggggggag caacttcgcg ggaatttcgt tcccgtttgt
    20161 ttgcgacaag atccacggtg aaaaaatcac cattttacct gtcgagccga ccgcctgtcc
    20221 tacgctgacg agaggacctt tcgcctatga tcacggggac gtcgcgaaaa tgacgccact
    20281 tctcgccatg cattcactcg ggcat
//
LOCUS       DAOSIO010000006        19995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_2942_length_19995_cov_4.225627, whole genome shotgun sequence.
ACCESSION   DAOSIO010000006 DAOSIO010000000
VERSION     DAOSIO010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 19995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19995
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2942_length_19995_cov_4.225627"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..586
                     /locus_tag="PK590_00555"
     CDS             248..586
                     /locus_tag="PK590_00555"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG23119.1"
                     /translation="MMKTLMLLLLFPILASSCANFQKSSYGSYAEAEKAFSRGKYEKA
                     IAGYRDYLEQNPGSSMAVIAEYYIGKSYAAMGMTGEAEKVYCDVIEKYPQSSWADFSQ
                     KRLDEIKLAV"
     gene            603..1027
                     /gene="rnpB"
                     /locus_tag="PK590_00560"
     ncRNA           603..1027
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK590_00560"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1169..1315
                     /locus_tag="PK590_00565"
     CDS             1169..1315
                     /locus_tag="PK590_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23120.1"
                     /translation="MKRQKARDYDPAKDGGVFLCLPGRKTGDGLGCSQAQRAQILASI
                     VGVA"
     gene            1315..1860
                     /gene="pyrR"
                     /locus_tag="PK590_00570"
     CDS             1315..1860
                     /gene="pyrR"
                     /locus_tag="PK590_00570"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012938850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOG23121.1"
                     /translation="MSAEKLVLDSASIDRALVRIAHEIFECHKAPQLLAVVGIRTRGV
                     YLAERLQKKLHEITGTEIPLGTLDITMYRDDLSEVGPNPEVRETKLPFDLNKRVVLLV
                     DDVLYTGRTVRAALDALNDYGRPKMIQLAVLVDRGHRELPIRADYVGKNVPTSVKERV
                     IIRIKEVDGKDEAVITGGNGI"
     gene            1857..2798
                     /locus_tag="PK590_00575"
     CDS             1857..2798
                     /locus_tag="PK590_00575"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOG23122.1"
                     /translation="MTAGVQWTRKDLLGLKDLSVDEIELILDTAKSFSEVSQRDVKKS
                     PALRGKTVVNLFLEPSTRTRVSFELAEKRLSADAIGMASGGSSMTKGESLWDTIANIE
                     ALKVDIIVMRHGSSGAPHRVSNYTHCGIVNAGDGINEHPTQGLLDIFTLREIKGKIKG
                     LKVVFVGDILHSRVARSNVWGLTKLGAEVVICGPKTLIPWGIEELGVKVVHDVKKAID
                     DADAVTLLRIQMERQNETLFPSIREYASTFGLTLQLFKRAKPDAILMHPGPVNRGVEI
                     EGALADSSQSYILKQVTNGIAVRMAVLYLLSGALKNE"
     gene            2791..4089
                     /locus_tag="PK590_00580"
     CDS             2791..4089
                     /locus_tag="PK590_00580"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008102165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOG23123.1"
                     /translation="MNKLNGQSLKNLLIKNARIIDPVNSIDEVMDLHVQSRKIVKWGK
                     NLQSDDAETMEAKGLCLMPGLVDLHVHFREPGYEHKETIESGVQAAIAGGFVACVAMP
                     NTRPACDNAQVVKYQMERANLLNFNLLPAGTLTQGREGKKISEMAEMKASGAVAVTDD
                     GNWVVDSGVARRAYEYAATYGLPVMTHAEDPTISLNGVMNEGIVSTKLGLRGRPNASE
                     DIATARDIELTRLCQCHLHVQHVSTRGAVDMIRRAKAEGLSVTAEATPHHIALTDTEF
                     VKYDTFYKMNPPLRTEEDRLAVIKGLEDGTIDAIATDHAPHALEEKDLDIEKAPFGTI
                     GLESAFGVVMTELYHGRKWKIGDVIRKLSVVPSEILKKTNLGRLKAGDHANFILVDLD
                     LEWCFEQKDIYSKSMNSCFFGKKLKGKVLHTFVKGFQYTL"
     gene            4313..7525
                     /gene="carB"
                     /locus_tag="PK590_00585"
     CDS             4313..7525
                     /gene="carB"
                     /locus_tag="PK590_00585"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOG23124.1"
                     /translation="MPRRKDIKKILIIGSGPIIIGQACEFDYSGTQACKALRKEGYEI
                     VLVNSNPATIMTDPEMADKTYIEPLTVETLEKIIALERPDALLPNLGGQTGLNLSSAL
                     YKSGVLEKYAVQIIGVKADAIERGEDREVFKATMAQIGIPVPQSATANSIEECEQIAQ
                     KIGYPVVVRPAYTMGGTGGGIAYNVEDLRVIALRGLTLSIARQVLIEEAVIGWEELEL
                     EVVRDSKNQKITVCFIENVDSMGVHTGDSFCVAPMLTVSAEIQKKLQEYAHKIVEAIG
                     VVGGTNVQFAHNKKDGRIVVIEINPRTSRSSALASKATGFPIAMVSTRLAAGLTLDEI
                     PYWRSGTLDKYKPSGDYVVVKFARWAFEKFSKAKDVLGTQMKAVGEVMSIGKTFKEAF
                     QKAIRSLEIGRYGLGGAKNFKQLSAEQLRERLAHPSSERIFLLYEALRQGFTVEELHR
                     MTHIGEWFIGEMRDLVMFEEEILKTSWDKFSDKDLIKAKEWGFADRYLAGLFGISETA
                     VRERRLGLGKIARYEAVPVSGVREAAYYYSTYLPGKEDSVVSKKKKIMILGGGPNRIG
                     QGIEFDYTCVHAVFALREAGYESIMINCNPETVSTDYDTANKLYFEPLTVEDVLAIYE
                     KEKPEGVIVQFGGQTPLNIAKQLKDAGVKILGTTPESIAFAEDRELFRQKMIELGIPQ
                     PESGIARSLDEALTISQNIGYPLMVRPSFVLGGRGMEIIYDEEMLRKYAAEAIKVSPE
                     HPMLIDQFLEMAIECEVDALADGEDSFVTTVMEHIEWAGVHSGDSACSIPSRTISSAH
                     LEEIRDYTTRIAKALKVTGLINVQYAICHDKVYILEANPRASRTVPIVSKVTGVPIAR
                     LATEIMLGKKLKDFPQLKKTVIPFVGVKEAVFPFNMFPEVDPLLGPEMRATGEVMGIG
                     PNFGIAFYKAQSATGAKLPLEGNVLLTVADKDKIALAPIAKKISALGFQIFATEGTAK
                     VLTARGIQNTLIKKINEGQPNIADAIKNGELHLIVNTPHGRGSQTDDSYIRTMAIQRK
                     LPYVTSLAAAEATAAGIAAAKKDKIEPKALQDYYKKSQKA"
     gene            7556..7723
                     /locus_tag="PK590_00590"
     CDS             7556..7723
                     /locus_tag="PK590_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23125.1"
                     /translation="MFKRTGIQKTLVTDSGSSVIGQTCEFDRSGGRAVRDDDLDVTSI
                     HDYHGLKSGRG"
     gene            7727..8500
                     /locus_tag="PK590_00595"
     CDS             7727..8500
                     /locus_tag="PK590_00595"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOG23126.1"
                     /translation="MVKKLFDEIVTIRENRRVNAKYRKLVFVSPELSRHVLPGQFLHV
                     KINQGRDPYLRRPFSYYRAQKNTVEILYEVLGHGTDVLSRKKKGDSLKIMGPLGNNFK
                     IDLKGKKRILVGGGIGVPPLVHLAERFPSDYLLMGTKSSAEVLPGNELKRVKAKILYA
                     TEDGSIGAKGFVTAPLEKIIKKENPQDLFIQTCGPKPMMLAVMAMARRYGVRGEASWD
                     EAMACGVGACLGCMVETTEGLKRACVDGPVFNFEELVGR"
     gene            8497..9465
                     /locus_tag="PK590_00600"
     CDS             8497..9465
                     /locus_tag="PK590_00600"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOG23127.1"
                     /translation="MKIDLTMRIKGLRLKNPVTVASGTFGTADEYAGFVNYRELGAII
                     TKTITVKPRSGNPMPRICEVSAGMLNAIGLQNRGLEDFVEHKIPYFKKLGVPLIVSIA
                     GESTKEFGLLAKAMGRFPSVVKALEVNLSCPNVEGGGVNFMRKQKRILDAIQAVRDNA
                     GVLVFAKLSPELGDVLQIAEKVLAKGTDGLSLINTLRGMSVHIKKRASRIVRDFGGLS
                     GPAIKPVALRYVYQAKKEFGVPIIASGGISNWQDAVEFLIVGADLLAVGTANFVEPMA
                     TMDILRGLKDFCRTEKIATLDALRGTYVPAEGGRKDANNECICSGA"
     gene            9440..10168
                     /gene="pyrF"
                     /locus_tag="PK590_00605"
     CDS             9440..10168
                     /gene="pyrF"
                     /locus_tag="PK590_00605"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020310987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOG23128.1"
                     /translation="MNVSAPARDKLIVALDAPSLDEARTWLESLQGVVSFYKVGLELF
                     TAFGWRAVDLVQKFGGKVFLDLKLHDIPNTVSRTLRSVCEHGVDIVNLHALGGYEMMS
                     KAGEAVQAFSAGTGKRPRLIAVTVLTSHSQEMISKEIGIKRKIRDEVLHLAKLARKAG
                     LDGIVCSPQETSMIRREFGKDFLIVTPGVRPAGSSAGDQQRIETPEKAVKAGADHIVV
                     GRPITQAADPRQAAADILAAISSL"
     gene            10270..11997
                     /locus_tag="PK590_00610"
     CDS             10270..11997
                     /locus_tag="PK590_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOG23129.1"
                     /translation="MAVIVKRVERPRELKWYQAGAMLYGDWGTSKAYVLGIAFALAGH
                     TSWFFLGLMSILTALVGVCYTMVCRAFPDGGGVYSSVKHRSQTLAVIGAFLLFANFAV
                     TSAISVLDAFHYFNFPNPEIWAIGVILLIGALNWVGPSKSGNVAAVVAVFASVVAVIL
                     FVAAVPQLGHIDLQMPSRDLGRNWATFVGIILAISGVEAIANMTGVMVEPVGKTSKRA
                     IFPVLIEVSILTFLLGLAMNAIPGLTGHTEDMLRVMGNHYIGPWFGKIVSIAFGFLLL
                     SAGNTAISALVSIQFTLAKDKELPPIFSRLNRFGMPWFALIVAVLVPVSVLIIERDLM
                     RLAALYAIGVVGAITLNLGTCAANPSIHLKKWERGLLAFTTMILVVIEITIAIEKQNA
                     LFFALTVLGVGLALRFLGKKVVLAPMPELASSVNLLTVQEAREISSLYKGTALVALKA
                     FNPALLEEAALHVKAKGENSVYLSYVEEVPPSPELPSEMEPSRESVELLGKAQEAMEK
                     HGITAVPIWQAGDNPGRMIARAARVLDVTTVVIGATKRSALINLLRGDVLRNLAHNLP
                     KASHLLIVG"
     gene            12056..12367
                     /locus_tag="PK590_00615"
     CDS             12056..12367
                     /locus_tag="PK590_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23130.1"
                     /translation="MKTAIIVFVLWLWVAIPPCFPEWFDGEVSKVDTRAQELIITEQD
                     PITEDEEATVISILSTTKFSGVKSLKEIKTGDEASAEVSYDELSDSWQAISVEVAGAG
                     A"
     gene            12364..13260
                     /locus_tag="PK590_00620"
     CDS             12364..13260
                     /locus_tag="PK590_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOG23131.1"
                     /translation="MRPRGPLPRCYTFGILTTVAKDIFRSSYHAELLSGVFKRAFEKH
                     CDLKIIPYRALLHTGISNILHRHSLDGLMIMTWRWIHPRFCQLIENTRDTRVLVVNDP
                     LPGLGVNIVQTDVAAGMRLAVNFLFQKNMRRIGMLHGPRHVTFRVRRKKVQIPFIDTQ
                     LKLNGFTKASGKRGIKVRKEWIRMTRANSESEGYRVMKKWLCDKDLPRAILCGNDDLA
                     FGAIKAVRERGFHCPGDMAVIGFDDNNRAGTHVPPLTTIRQPLMKMGEDAVNILLGKI
                     GRPGSAAISKKYLPQLIIRKSA"
     gene            13388..14179
                     /locus_tag="PK590_00625"
     CDS             13388..14179
                     /locus_tag="PK590_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA
                     nucleotide-binding domain protein"
                     /protein_id="HOG23132.1"
                     /translation="MLITIIAALIGLGYFFYPPRFAWVRASVKYLIGVFCFWAALVFA
                     RPEVQEPLWRGAGVFILLIAGLLLLLDGVTAFSIWIADRVSLLFKRKLKLPGYLEEIN
                     TAFHHLAARKVGALIVLQQSRPLKSHMRGPGLPFDAEIRSEILIPLFLTTSPVHDGAV
                     IVHKGRIQTVKAILPLATLVEGHVNFGTRHRAAIGITERTDAVAMVVSEERGEISIAS
                     DGCLVRIPNQEEFVRVMNWLLKGRSILKLKGVRFVITAKVLEDFA"
     gene            14246..15712
                     /gene="glpK"
                     /locus_tag="PK590_00630"
     CDS             14246..15712
                     /gene="glpK"
                     /locus_tag="PK590_00630"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOG23133.1"
                     /translation="MKRYILAIDQGTTSSRAVLFDLAGAVVGSAQQEFRQYFPRPGWV
                     EHDAEEIWRSCVSVIRKAVARSRIPPSAIAGIGITNQRETTVLWDRKTGRQVYRAIVW
                     QCRRTSALCARFKKYQPVFRKKTGLLLDPYFAGTKIRWLLDHVPGLQKRAANGEIAFG
                     TIDSWLIWKLTGGREHVTDLTNASRTLLLNIRTKKWDPELLDILGVPSPILPKVKRSG
                     SVFGRMVGVAGLPAGIPIAGVMGDQQAALYGQGCHRSGTSKNTYGTGCFIVLNTGKKF
                     VISKAGLLTTLACDARGRPVYALEGSVFIAGAVIQWLRDGLKVIRHSSQTEKMIRGLE
                     ETHEVYVVPAFVGLGAPYWNRHARGIISGITRGTTASHIIRASLESIAYQTRDVYDAM
                     RKDVGKHIRELRVDGGACRNNFLMQFQADILGAKVVRPKVIELTVQGAADLAAVTLGL
                     KKPSGIAKIDKVFYPSMSKGERERLCQGWMKAVKRAML"
     gene            15793..17271
                     /locus_tag="PK590_00635"
     CDS             15793..17271
                     /locus_tag="PK590_00635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOG23134.1"
                     /translation="MIRDINQFEKNQYDLLVIGGGINGASIANLSARAGIKTALLEKG
                     DFASGTSSKSTKLIHGGLRYLENLEVDLVYESLKERKVQLAVAPHLVQPLEFLIPVYQ
                     GDRRPLWMMRLGVFLYDLIAGPYRIKRHKALNATEVLRLEPGLQRKGLKGGVVYYDAQ
                     MDDARLCLENVLSASEAGAHAANYAEVKEYLKENGRVIGVKARDHIGNRNFEVHAKKV
                     VVAVGPWTDKLLKLDDLRAPQKVRTTQGIHIVYKDKLSGRALFVPSQSDKRIFFVIPW
                     LGHSLIGTTDTDYVGDPDRVKVSPDDVEYLMRETRRVFPELDMSPRKILSTFSGLRPL
                     VRKGGSASKVSRKHLFYETPSGLIFVVGGKYTTYRAVAESCLKKIFKRKTWPFALCDR
                     GKSPGSMDDVARSFNLEPETVHSLFGIYGGRVQDVLAMIAKEPSLKEKVSFDPPVIKA
                     QLLYAVQKEMACTVEDITDRRLSLGYLGPVPESVLATIREFL"
     gene            17794..19188
                     /locus_tag="PK590_00640"
     CDS             17794..19188
                     /locus_tag="PK590_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23135.1"
                     /translation="MSASTGHEGMKVGCSLIDGLWGKKELSGGGLVVQKGALMRIIVL
                     SNNPWSNNNSFGNTFSNLFQGMPDIKIANIYCKHGIPQNHIVDHHFQITEKMLIKNYL
                     ARKNLSGREIFQDCNAERLEHKERTIVDYARKKRWMIFFWLREIIWALGRWKSPELVS
                     FITRFNPDIIFKPLYSSVYMSKIALFVKGYTKKPMVCYATDDVYTWHQFSLSPLYWID
                     RLIKRKFIRDVVKQCEMLYVISDVQKEEYGKCFGKPCKTLTKGAEFDRPLYKSCLNNP
                     LKFVFTGNVSAGRWKSLALIGDSLEKINKNGIRAILYIFTETPFSKKMLHRLQSRSIR
                     VMNPVPAREIQSIHADADVLVHVESFSLKDRLVVRHSFSTKLVDYFHAARCVFAVAPP
                     DVASMCYLLKNDSAVVATSEHDVIIQLRHIIENPKVVHQYARKAWSCGKKNHLIKNIQ
                     KCLREDLNNLVMKE"
     gene            19195..>19995
                     /locus_tag="PK590_00645"
     CDS             19195..>19995
                     /locus_tag="PK590_00645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG23136.1"
                     /translation="MKVLQINAVSGIRSTGVSCVQLADYLNQNGHEGFIAYGSGLPYI
                     KGHKIGNAFDAKAHALLSRITGKQAYFSVRATEKLLGYMIQIKPDVVHLRNLHSSYVN
                     LKRLLRFLSQQDIPTVITLHDCWFYTGKCTHYTENRCFRWKNQCGNCPRLKRDNPSWF
                     FDRTAQMLKDKKSWLTQIPRLAVVGVSDWIVSEARQSFLSSASIVARIYNGIDLEVFR
                     PQPVDYLKKKHGLDGQFVILGVASRWCDSKGLNKFVMLAKTLPRDMRII"
BASE COUNT         4938 a         4402 c         5583 g         5072 t
ORIGIN      
        1 ttacgaaaga agtcggcgcg gatgattttt tgatcaagcc gtttgagacc gagcagcttt
       61 tggaaaaggt taaaaacgct atttttaagg aaaaccaata atctcctcgt gctgtttgcc
      121 tgaagaacct ggctgatttt tcaaggccta tttcattttt atgcccttga ccctgtttgg
      181 cttgccaagt aaacttgcgt ccaaggggaa ttggtaagga aaacctcaga ggtttgtgac
      241 tatggacatg atgaagacgt tgatgctttt attgcttttc ccgatattgg cttcttcctg
      301 cgcgaatttt cagaaatcaa gctacggaag ttacgcggag gcggagaagg ctttcagccg
      361 cggcaagtat gaaaaggcca ttgcggggta ccgggattat cttgaacaga acccgggtag
      421 cagcatggcg gttattgcgg aatattatat tgggaaaagt tacgcggcca tggggatgac
      481 cggagaggct gaaaaggttt attgtgacgt cattgaaaaa tatccccaat cctcatgggc
      541 tgatttttcc caaaaaaggc tggatgaaat aaaattagca gtttagattc tgttctttga
      601 gaagatcttc ctggacggtc gctcgcggaa acggtaaaca ggaaacgacc tgtaggacat
      661 cgtgggagga aagtccgggc ttcgcagggc atcgtagtgg gtaacgccca tccttctgcg
      721 gtttgtccgt taaatctttc agatttaacg aaacggacca cggaagcgga tcagagccac
      781 agagacgagt ccctgacgca agtcaggggg tgaaacgcgg caatctctac gtgaagaaag
      841 gctaaatagg gtgagatacc tgaaaaaggc ggcattgccc gtgccggtct ccgaacagga
      901 gcgtctcatc gggtcagccg aatgagctcc gcagtaatgc ggagtcaaga tagatgaccg
      961 tcacctcgtt gttttgcttg tcgaagcaac agggggacag aacccggctt acaaggaaga
     1021 tcttctcttt ttttcgctct tttctttgtt caggaaattg gcatggactg tccttgacaa
     1081 acgaaacagg agcgtatatc cttgcgataa gtttgagtgg tacaggatcc ttttaagacg
     1141 gcgcggagac gctgacaaag gtgagaaaat gaaaagacaa aaagcgcgtg attatgaccc
     1201 tgccaaggat ggcggggttt ttttgtgcct gccgggacgt aaaaccggtg acgggttggg
     1261 gtgctcgcaa gctcaaagag cgcaaattct ggccagcatt gttggtgtcg cgtgatgagc
     1321 gccgagaaac ttgttttgga tagtgccagt atcgaccgcg ctcttgtccg catcgctcac
     1381 gaaatttttg agtgtcataa ggcccctcag cttctcgcgg tcgtgggtat tcgcacccgt
     1441 ggcgtgtatc tcgcggaacg tcttcagaaa aaacttcatg aaattacggg gactgaaatt
     1501 cctctcggga cactggatat caccatgtat cgcgacgacc tcagcgaagt aggccccaac
     1561 cctgaggttc gtgaaacgaa acttcctttt gatctgaaca aaagagtcgt gcttctcgtg
     1621 gatgacgtgc tgtacacggg gcgaacggtt cgcgcggccc tggacgctct caacgattat
     1681 ggacgcccga agatgattca gctggcggta ttggtggatc gcggccaccg tgagcttccg
     1741 atccgggcgg attatgtcgg gaaaaatgtt cctacgtcgg tcaaggagcg tgtgatcatt
     1801 cgaatcaaag aagtggacgg aaaagatgaa gctgtcatca cgggagggaa cggcatatga
     1861 ccgctggcgt gcagtggacc cgcaaggatt tgttagggct taaagattta tcggtcgacg
     1921 aaattgaact tattctggat acggcgaagt ccttcagcga agtttcccag cgcgatgtca
     1981 aaaagtcgcc ggccctcaga ggcaaaaccg tggtgaacct ttttctcgaa ccgagcaccc
     2041 gaacgagagt gtcgtttgag ctcgccgaaa aaagattgag cgcggacgcg atcggcatgg
     2101 ccagtggcgg atccagtatg acgaagggag agtccctttg ggatacgatc gcgaatatcg
     2161 aggctctcaa ggtcgatatt atcgtgatgc ggcacggttc ttcgggggcg ccgcaccgtg
     2221 tttccaatta cacgcactgt ggaattgtta atgccggtga tggaattaat gagcatccga
     2281 cccaggggct tctggatatt ttcacgcttc gcgagattaa aggaaagatc aagggactga
     2341 aggttgtttt tgtcggggat atcctgcact cgcgagtggc ccgttccaat gtctggggtt
     2401 tgactaaatt gggcgccgaa gttgtgattt gcggtcctaa aacgttgatc ccctggggta
     2461 ttgaagaact gggtgtgaaa gtggtccacg acgttaaaaa agcgatagac gatgcggacg
     2521 ccgtgacgct tcttcgaatt cagatggaac gacaaaacga aacgcttttt ccaagtatcc
     2581 gcgagtacgc aagcaccttt ggcctcactt tgcaactttt taagcgcgcg aagccggacg
     2641 ccattcttat gcatccgggg ccggtcaatc gcggcgtaga aattgaagga gcgctggcgg
     2701 atagttccca gtcctacatt ctcaaacagg tcacgaacgg tatcgcggtg cgtatggcgg
     2761 tgctctacct tcttagcggg gcattgaaaa atgaataagc tgaacggaca gtcgcttaaa
     2821 aatcttctga tcaagaacgc gcggattatt gatccggtca attcgatcga cgaagtcatg
     2881 gaccttcatg ttcagagccg caaaatcgtc aaatggggaa aaaatctcca gtcggatgat
     2941 gccgaaacga tggaagcgaa gggactttgc ctgatgccgg ggctggtgga tttgcacgtg
     3001 cattttcggg agccgggcta tgaacacaag gaaaccatcg aaagcggggt ccaagcggcg
     3061 attgccggag gatttgtggc ttgtgtcgcg atgccgaaca ccaggccggc ttgtgacaat
     3121 gcccaagtgg tgaagtacca gatggagcgc gcgaatcttc tcaacttcaa ccttttgccg
     3181 gcagggaccc tgacccaggg gcgcgaaggg aaaaaaatat cggaaatggc cgagatgaaa
     3241 gcctcggggg cggtggccgt aacggacgac gggaactggg ttgtggattc cggggtggcg
     3301 cgcagggctt atgaatacgc cgctacttac gggcttccgg tcatgaccca tgccgaagat
     3361 ccgaccattt ccctgaatgg tgtgatgaat gaagggattg tctcaacgaa gttgggactc
     3421 cggggtcgcc cgaatgcctc tgaagacatt gccacggccc gggacattga attgacccgg
     3481 ttgtgtcaat gccatcttca tgtccagcac gtgtccacgc ggggagcggt ggacatgatc
     3541 cggcgcgcga aggccgaagg actttccgtg actgccgaag ccacgccgca tcacattgct
     3601 ctgacggaca cggagtttgt gaaatacgat actttttaca aaatgaaccc gccgcttcgc
     3661 accgaagagg atcgcctggc ggtgatcaag ggacttgaag acgggacgat tgacgcgatc
     3721 gcgacggatc acgcgccgca cgctcttgaa gaaaaagatc tggatattga aaaggcgccg
     3781 ttcggaacca tcggtcttga atcggccttt ggggtcgtga tgacagagct ctatcacggg
     3841 cgaaagtgga aaattgggga tgtgatcaga aaattaagcg tggtcccgag tgaaattctg
     3901 aagaaaacaa acttggggag gctcaaggcc ggagaccatg ccaattttat cctggtggat
     3961 ttggatctgg aatggtgctt tgaacaaaaa gatatttatt caaagtcgat gaattcatgt
     4021 ttctttggaa aaaaactcaa ggggaaagtc ctccacacgt tcgtcaaagg ttttcaatat
     4081 accctttgaa gacgcggaat cttcccgggt atggcccgaa gaattggttg ttaaagagct
     4141 agccccacgg tgagtcattg tcgctccgag atgacgggtg ggttttatcc ccgggcagtg
     4201 cgatatcccc agaatccaga ggatgggcgc ccgcgcaaac gatggcgatt atcttttgaa
     4261 cagtttttat caacttattt gaacacgcag gataaaaaga acgaggaaaa atatgccgcg
     4321 gcgaaaagat attaagaaaa ttctgattat tggttcaggt ccgatcatta ttggccaggc
     4381 ctgtgagttt gattattcgg gaacgcaggc ttgcaaggct ctccgcaagg aaggctacga
     4441 aattgtgctt gttaattcta atccggcgac gattatgacc gaccctgaaa tggcggataa
     4501 aacttacatt gaacctttga cggtcgaaac gcttgaaaaa attatcgccc tagaacggcc
     4561 cgacgctctt ttgcccaatc ttggcggcca gacgggcctg aacctgtcct cggcgcttta
     4621 caaaagcgga gtattggaaa agtacgcggt tcaaattatc ggggtgaaag cggatgccat
     4681 cgagcgggga gaggatcgtg aggtttttaa agcgacgatg gcccagatcg gaattccggt
     4741 cccccagtcg gcgacggcca attcgattga agaatgcgaa cagatcgcgc aaaaaattgg
     4801 ctatccggtc gtggttcgtc cggcgtatac gatgggcggt accggtggag ggattgctta
     4861 taacgtcgaa gatcttcggg tgattgcctt gcggggattg acccttagca tcgcgcggca
     4921 ggtactcatt gaagaggcgg tgattggctg ggaggaattg gagcttgaag tggtgcgcga
     4981 ttcgaaaaat cagaaaatca cggtttgttt tatcgaaaat gtagattcca tgggggttca
     5041 cacgggtgac agcttttgcg tcgccccgat gctgaccgtg tcggccgaga tccagaagaa
     5101 actccaggaa tatgcccaca agattgtgga agcgatcgga gtggtggggg ggaccaacgt
     5161 tcagttcgcg cataataaaa aagacggtcg aattgtcgtc attgagatta atccgcgcac
     5221 gtcacgttcc tcggcccttg cttccaaagc gacgggattt ccgatcgcga tggtctcgac
     5281 ccgtttggcg gcgggattga ccctggatga gattccctat tggcggagtg gaactctcga
     5341 caagtacaag cctagcgggg attatgtggt cgtcaagttc gcgcgatggg cttttgagaa
     5401 attttccaag gcgaaggatg ttctcgggac ccagatgaag gcggtcggcg aggtcatgtc
     5461 gatcggcaag actttcaagg aagcatttca gaaagccatc cgctcgcttg aaattggccg
     5521 ttatggtttg ggaggcgcga agaattttaa acagctttcc gccgagcaat tgcgggaacg
     5581 gcttgcccat ccttccagtg aaagaatttt cttgctgtat gaagcgcttc ggcaaggttt
     5641 tacggttgag gagcttcacc gtatgacgca tatcggggag tggtttatcg gagagatgcg
     5701 agatcttgtg atgtttgagg aagagatttt gaaaacgtca tgggataaat tttccgacaa
     5761 ggatttgatt aaggccaagg aatgggggtt tgccgaccga tatcttgcgg gacttttcgg
     5821 aatttccgaa accgcggtca gagaacgtcg cttgggtctg gggaaaatag cgaggtatga
     5881 ggcggtgccg gtcagtgggg tgcgggaagc ggcttactat tattccacct accttccggg
     5941 caaggaagat tcggttgtga gcaagaaaaa gaaaatcatg atcctgggcg gcgggcccaa
     6001 ccggatcgga caagggattg agttcgatta cacgtgtgtc cacgcggttt ttgcgcttcg
     6061 tgaggcgggt tatgaatcca tcatgatcaa ttgcaacccc gagacggttt ccaccgatta
     6121 cgataccgcg aacaaacttt actttgaacc gctcacggtg gaagacgtgc tcgcgattta
     6181 tgaaaaggaa aaaccggaag gcgtgattgt ccagtttggg ggtcaaacac cgcttaatat
     6241 cgccaagcaa ctgaaggacg cgggagttaa aattctgggc acgacgccgg aaagtattgc
     6301 tttcgcggaa gaccgtgagc tttttcgcca gaagatgatt gagcttggaa tccctcaacc
     6361 tgaaagcgga attgcgaggt cgctcgacga agctctcacg atttcacaaa acatcggata
     6421 cccgctcatg gtacgccctt cctttgtctt gggcggtcgc gggatggaaa ttatatacga
     6481 tgaagaaatg ctcaggaaat atgcggcgga ggccatcaag gttagtcccg aacatccgat
     6541 gctcatcgac cagtttcttg agatggccat cgagtgcgaa gtggacgcgc tggcggatgg
     6601 ggaggattct tttgtcacta ccgtgatgga gcatatcgaa tgggcgggtg tccattcggg
     6661 agattcggca tgttcgatac caagtcgcac catttccagc gctcacctgg aagaaatacg
     6721 ggactatacc acgaggatcg cgaaggccct taaagtgacg ggcttgatca atgttcaata
     6781 cgccatctgt catgacaagg tctacatcct cgaagctaat ccgcgcgcct cccgcacggt
     6841 cccgattgtt tccaaagtga ccggagtgcc gatcgcccgg ctcgcgaccg agattatgtt
     6901 ggggaaaaag ctgaaggatt tcccacaact gaaaaaaacc gtcattcctt tcgtgggggt
     6961 gaaagaagct gttttccctt ttaacatgtt cccggaggtg gacccgcttc tgggacccga
     7021 gatgcgcgcg accggagagg tgatggggat cgggcccaat tttggaattg cgttctacaa
     7081 agcccagtcc gcgaccgggg caaaactccc gcttgaaggc aatgtgcttt tgaccgtagc
     7141 ggataaggac aagatcgcct tagcgccgat tgccaaaaaa atttcggccc tcggtttcca
     7201 gatttttgcg acggaaggga ctgccaaagt tttgacggcc cgcgggattc aaaacacact
     7261 gatcaagaag attaatgaag gacagcccaa catcgcggac gcgatcaaaa acggcgaatt
     7321 gcatttgatt gtaaatacac cgcacggacg cggaagccag accgatgaca gttatattcg
     7381 aacgatggct attcagcgca agttgcccta tgtgacatcg ctggcggcgg ctgaagctac
     7441 cgcggccgga atagccgcgg cgaagaagga taaaatcgaa ccgaaggcct tgcaggacta
     7501 ctataagaag agccaaaagg cataagtttt ttttcagaag cgctggagaa attccatgtt
     7561 taaacgaacc ggcatccaga aaaccctcgt gacagattcg ggatcaagtg ttatcggtca
     7621 gacctgtgaa tttgatcgtt cgggtggccg ggctgtgaga gatgatgatc tggacgtgac
     7681 gtcaatccac gactatcatg gtctcaaatc gggcaggggc taaatcatgg tcaagaagct
     7741 ttttgatgaa attgtcacta ttcgggaaaa caggcgggtg aacgcgaaat atcgcaagct
     7801 tgtttttgtt tcgcccgaac tttcccgtca tgttttgccc gggcagtttc ttcatgtcaa
     7861 aattaatcag gggcgggacc cttatctccg acgacctttc agctattacc gcgcgcaaaa
     7921 aaataccgtt gagatccttt acgaagtttt aggacatggc accgatgtct tgtcccgaaa
     7981 aaagaagggg gattctctaa aaatcatggg accgcttggc aataatttta aaatagacct
     8041 gaaaggaaaa aaacggatcc ttgtcggagg cggcattgga gtccctcctc ttgtccatct
     8101 ggcggagcgt tttccttcag attatctttt aatgggcaca aaatcttcgg cagaagttct
     8161 gcccggaaac gagctgaaaa gggtcaaggc caagattctt tacgcgacgg aagatggctc
     8221 aatcggcgcg aaaggttttg tgacggcgcc tcttgaaaag attatcaaaa aagaaaatcc
     8281 ccaggatctt tttatccaaa cttgtggacc gaagccgatg atgctcgcgg tcatggccat
     8341 ggcgaggcga tatggagtgc ggggagaagc ctcttgggat gaagcgatgg cttgcggagt
     8401 gggggcttgt cttggttgta tggtcgaaac gacggaaggg ctcaaacggg cttgcgtgga
     8461 tggcccggta tttaattttg aggaactggt gggccgatga agatagatct tacgatgcgg
     8521 atcaaggggt tgcggctcaa gaatcccgtg accgttgcgt ccgggacctt tggcacggcg
     8581 gatgaatacg cggggtttgt gaattacaga gaacttgggg ccatcattac aaagacgatt
     8641 accgtcaagc cccgctccgg aaaccccatg cctagaattt gtgaggtttc ggccggaatg
     8701 cttaacgcga tcggtcttca aaacagggga cttgaagatt ttgtcgaaca taaaattccg
     8761 tatttcaaaa agctcggggt gccgctgatc gtaagtatcg ccggagaatc gacgaaggaa
     8821 tttggccttt tggcaaaagc catgggacgt tttccgtccg tcgtgaaggc gcttgaagta
     8881 aatctttcat gtccgaatgt tgagggcggc ggggttaatt tcatgcgaaa acaaaagcgg
     8941 atcctggacg ctatccaggc cgttcgcgac aacgcggggg ttttggtgtt tgccaaacta
     9001 tctccggaac tgggtgatgt tttgcagatt gccgaaaaag ttttggcgaa agggacggac
     9061 ggtttaagtc tgatcaatac tcttcgcggg atgtcggttc acattaaaaa gcgcgcatca
     9121 agaattgtca gggattttgg ggggcttagc ggacccgcga tcaaaccggt tgccttgcgc
     9181 tacgtttacc aggccaagaa ggaatttggt gttcccatta ttgcctcagg gggtatctcg
     9241 aactggcagg atgcggtaga gtttctcatc gtaggggccg atcttctcgc ggtggggacg
     9301 gctaattttg tggagccgat ggccactatg gatattttga gagggttaaa agatttttgc
     9361 aggaccgaaa agattgcaac gctggatgct ttgcgcggaa cctacgtgcc tgccgaaggg
     9421 ggacggaaag atgccaacaa tgaatgtatc tgctccggcg cgtgataaat taattgtcgc
     9481 cttggacgcg ccgtctcttg acgaagctag gacctggctt gagtcgcttc agggggttgt
     9541 gagtttttac aaagtgggtc ttgagctttt cacggcgttt ggctggcgtg ctgttgatct
     9601 tgtccagaaa tttggaggaa aagtttttct tgatctcaaa ctgcatgata tccccaatac
     9661 ggtcagtcgg acgctgaggt cggtttgtga acacggagtt gatattgtga atcttcatgc
     9721 cctgggcggc tacgagatga tgagcaaggc aggggaggct gtgcaagcct tttcggccgg
     9781 aacggggaaa cgccccaggc tgatcgccgt gacggtgctg acaagtcaca gtcaggaaat
     9841 gatttccaaa gagattggga tcaagcgtaa aatccgggac gaagtacttc atcttgccaa
     9901 gctggcccgg aaagccggtc tcgacggaat tgtttgctct ccccaggaaa cttccatgat
     9961 tcgccgtgaa ttcggaaaag attttcttat tgtgaccccc ggtgttcgac ccgcgggaag
    10021 ttccgcggga gatcagcaac gtatcgaaac tcccgaaaaa gccgttaagg ccggcgctga
    10081 tcacattgtg gtaggccgac ctatcaccca agcggccgat ccccggcagg cggcggctga
    10141 tattctagcg gccatctctt cattatgact tgcgcgggaa tcttgacccg cttatttcca
    10201 gtgagttagg ctttgcgaaa gatgtttatt ttgaaacgat atggctttta atctacaatc
    10261 aaaaaatcta tggccgtcat agttaaacgg gtcgaaagac ctcgcgaact taagtggtat
    10321 caggctggcg ccatgctcta tggtgactgg ggcacgagca aggcgtatgt acttggtatt
    10381 gcctttgccc tggccggtca cacctcgtgg tttttcctgg gattgatgtc cattttaacg
    10441 gcactggtgg gtgtttgtta caccatggtt tgccgcgctt tcccggatgg cggcggggtt
    10501 tactcttcgg ttaagcaccg atcccagaca ctggcggtga tcggcgcgtt tcttcttttc
    10561 gctaatttcg cggtgacgtc cgcaattagt gttctggatg ctttccatta ttttaacttt
    10621 cccaatcccg agatttgggc gattggggtc attttgctga ttggagccct caactgggtt
    10681 ggtccatcaa aaagcggaaa cgtcgccgcc gtagtcgcgg tttttgcttc cgtggtagcg
    10741 gttattcttt ttgtcgccgc ggtacctcag ctcgggcata ttgatttgca gatgccgtca
    10801 agggatttgg gacggaattg ggccacgttt gtggggatca tcctggcgat ctcgggggtc
    10861 gaagcgattg cgaacatgac cggggttatg gtggagccgg tcggcaagac ttcgaaacgc
    10921 gctattttcc cggtgcttat cgaagtctcg atcctgacgt tccttctggg actcgccatg
    10981 aatgccattc ctggcctcac gggtcacacc gaagatatgc tacgggtcat gggaaatcat
    11041 tacattggac cgtggtttgg caagattgta tcgatagctt ttggatttct tttgctctca
    11101 gcggggaata cggccatctc agctcttgtc agcattcagt tcactctcgc caaagacaag
    11161 gagcttccac cgattttttc cagactcaac cgttttggca tgccgtggtt tgctcttatc
    11221 gtggcggttc tggtgcctgt tagtgttttg ataattgaac gggatctgat gcgtctggcg
    11281 gcgctttacg cgattggggt tgtgggcgcg attacgctaa acctcggtac ttgcgcggcc
    11341 aatccgtcca ttcatcttaa aaaatgggag cggggactgc tggcattcac cacgatgatt
    11401 ctggtcgtga tcgagattac catcgcgatc gaaaaacaaa acgccttgtt cttcgcttta
    11461 acggtacttg gggtcggtct tgcgcttcgc ttcctgggga agaaagtggt gttggcgccg
    11521 atgcccgaac tggcatcgtc cgtcaacctt ttgaccgtcc aggaagccag ggagatttcg
    11581 tcgctttata agggcacggc gctggttgca ttaaaggctt tcaatcctgc gttgctggaa
    11641 gaagcggcgc tccacgtgaa agccaaaggt gagaattcgg tgtatctcag ctatgtcgag
    11701 gaggtccctc cgtctcccga gcttccgtcc gaaatggaac cgtcccggga atcggttgag
    11761 cttctcggca aagcccagga agcaatggaa aagcacggaa ttacggctgt tccaatttgg
    11821 caggcggggg acaatcccgg aaggatgatt gcccgggcgg cccgggtact ggatgtgacc
    11881 acggtggtca ttggagcgac caaacgaagc gctctcatta atctgcttcg tggcgatgtc
    11941 ctccggaatc tcgcgcataa tctgcccaag gccagtcatt tgctgattgt cggatagcct
    12001 tttttccgtt atcttgtacg cgctttaaaa acctggctcg aaggagtgtc tcacgatgaa
    12061 aaccgcgatc attgtttttg ttttatggct gtgggtggcc attcctcctt gtttcccgga
    12121 atggtttgac ggagaggtct caaaagtgga tacccgcgcg caagaattga tcataacgga
    12181 acaggaccct atcaccgagg acgaggaagc gacggtgatt tccattcttt ccacaacgaa
    12241 gttttccggc gtcaaatcct tgaaagagat caagacgggt gacgaggctt cggcggaagt
    12301 cagctacgat gagttaagcg attcctggca ggccatttct gtcgaggtgg cgggagctgg
    12361 cgcatgagac ctcgcgggcc gcttccgcgc tgttacactt ttggaattct tacgactgtc
    12421 gcgaaagata tttttcgttc cagttatcac gcggaacttc tctcgggtgt ttttaagcgg
    12481 gcttttgaaa agcattgtga tttaaaaatc attccgtatc gagcgcttct tcacaccggt
    12541 atttcgaaca ttctccatcg gcattctctg gatggattga tgatcatgac ctggcgctgg
    12601 attcatcccc ggttctgtca actgatcgaa aacactcgcg acacacgggt gctcgtcgtg
    12661 aacgatcctt tgccgggtct tggtgtcaat atagtccaga ccgatgtcgc cgcagggatg
    12721 cgacttgccg ttaattttct ttttcaaaaa aacatgcgca ggatcgggat gcttcatggc
    12781 ccccggcacg tcacgtttcg ggtccggcgc aagaaagttc aaattccctt cattgatact
    12841 caacttaaat taaatggttt taccaaagcg tccgggaaaa ggggaattaa agtaagaaaa
    12901 gaatggattc gtatgacgcg ggccaacagc gaatctgaag ggtatcgcgt gatgaagaaa
    12961 tggctttgcg ataaggacct tccgcgagcg atcctttgcg gaaatgatga tcttgctttt
    13021 ggcgcgataa aagctgtgcg tgaacgcgga tttcattgtc cgggagacat ggcggtaatt
    13081 ggttttgacg acaataatcg cgcggggaca catgtccctc cgctaaccac gatccgtcag
    13141 cctctcatga aaatgggaga agacgcggtg aatatccttc ttggaaaaat cggtcgcccg
    13201 ggatctgccg ccatttccaa aaaatatctg ccccaactga ttattcgaaa aagcgcctga
    13261 atattggctg ttggataaaa tgcaacaacc ccttgagaat caccggctac cgcgcccgtt
    13321 gtgttcacct tcataaaact tgccaaaggc cgtaaatata aagtaaaaat ttcacaaaag
    13381 gaaaatcatg cttatcacaa taattgcggc tctgatcgga ctgggctatt tcttttatcc
    13441 tccgaggttt gcctgggttc gcgcgtccgt caagtacctg atcggtgttt tttgtttctg
    13501 ggcggcgctg gtcttcgctc gtcccgaagt ccaggagccc ttgtggcgtg gtgccggagt
    13561 ttttatcctt ttgattgccg gattgctgtt gctactggat ggcgttacgg cattttcaat
    13621 atggatagct gaccgggtgt cgctgctatt taaacggaaa ctgaaattac cgggctatct
    13681 tgaagaaatc aatacggctt ttcaccacct cgcggctcgc aaggttggag cccttattgt
    13741 tttgcaacaa agccgtcctc tcaaatcgca tatgagaggc ccagggcttc cgtttgacgc
    13801 cgagattcgt tccgaaattc ttatcccgct ttttcttacg acatcaccgg tccatgatgg
    13861 cgccgtgatt gttcataaag gccgtatcca gacggtcaag gccattctgc cgttggcgac
    13921 gctggtggaa ggccatgtga acttcgggac ccgtcaccgg gcggccatcg gaattaccga
    13981 acgcaccgat gccgtcgcga tggtggtttc cgaagaacgg ggggagatca gtatcgccag
    14041 cgatggctgt cttgtccgca ttccgaatca ggaagagttt gttagggtga tgaactggct
    14101 tctgaaaggg cgtagcattc tcaaacttaa aggtgtccgg tttgtcatca cggccaaagt
    14161 gctcgaggat tttgcctgaa gatgaccgga tcgtttttgg gattttttta tttgtccttg
    14221 ctggaaaatt gagatgggaa atcctatgaa acgctatatt ctcgcgattg atcaggggac
    14281 gacaagttcg cgagcggttt tgttcgatct tgccggagct gtggtgggtt ccgcccagca
    14341 ggaattcaga cagtatttcc ctaggccggg ttgggtcgaa catgacgctg aagagatctg
    14401 gcgatcttgt gtttcggtga ttcggaaagc ggtagcccgg tctcgaattc caccttccgc
    14461 gatcgcggga attggaatta ccaatcaacg ggaaaccacc gttttgtggg accgaaaaac
    14521 gggacgacag gtttaccggg cgattgtctg gcagtgccgc cggacgagcg ctctatgcgc
    14581 ccgatttaaa aagtatcagc ctgtttttag aaaaaaaaca ggtcttcttc tcgaccccta
    14641 tttcgcgggg acgaaaatcc gctggcttct tgatcatgtc ccgggccttc aaaaaagggc
    14701 ggctaacggc gagatcgcct tcgggacgat cgatagctgg cttatctgga aattgacagg
    14761 aggcagggag catgtaacgg atcttacgaa tgcttcacga actctccttc tgaacatccg
    14821 cacaaaaaaa tgggatcccg agcttcttga tattctcggt gttccgtccc cgatacttcc
    14881 gaaagtgaaa cggtctggct cagtgtttgg ccggatggtg ggggtggccg gacttccagc
    14941 ggggattccg atcgccggtg tcatgggcga ccagcaggcg gcgctttacg ggcagggttg
    15001 ccatcgttcc ggcaccagta agaacactta tggaacaggg tgctttatcg tcttgaatac
    15061 aggtaaaaaa tttgtcatct ccaaagcggg acttttgacg acccttgctt gtgacgccag
    15121 gggccgtccg gtttacgcgc tggaggggtc ggtattcatt gcgggcgccg tgatccaatg
    15181 gctccgtgac ggtctgaaag tgatccgcca ttcttcgcag acggaaaaga tgatcagggg
    15241 acttgaagag acacacgaag tttatgttgt cccggctttt gtgggactcg gagcgcctta
    15301 ctggaacagg catgcccggg ggattatcag cggaattacg cgaggtacca cggcttctca
    15361 tattatccgc gcttcacttg agtcgatcgc gtaccagacc cgggacgttt acgatgccat
    15421 gagaaaagat gtcgggaaac acatccggga gctcagggtg gatggtgggg catgccggaa
    15481 taattttctt atgcaatttc aggcggacat tttgggagcg aaagttgtcc gtcccaaagt
    15541 gattgagctg accgtccagg gcgccgccga tcttgcggcc gtgaccctcg gtctgaagaa
    15601 gccttccggg atcgcaaaaa tagacaaggt tttttacccg tcgatgtcaa aaggtgaacg
    15661 ggaacgtctt tgccaaggat ggatgaaagc ggtgaaaagg gcgatgctct aaattaggtc
    15721 gcgatccagg cgttaacgcc catatttttt ggatcttggc cgttgcccgt ggatctgtgg
    15781 gggggggatt cagtgatccg ggatattaac cagtttgaaa aaaaccagta tgatctgtta
    15841 gtgattggcg gtggaattaa cggcgcctca atcgcgaatc tttctgcccg ggcgggtatc
    15901 aaaacggcgt tacttgagaa aggggatttc gcgagcggaa cttccagtaa atcgaccaag
    15961 cttatccacg ggggacttcg atatctggaa aatctggaag tggatctcgt ctatgagtct
    16021 cttaaagaaa gaaaagttca gcttgccgtt gccccccacc tggttcagcc tctcgaattt
    16081 cttattcccg tctaccaggg agaccggcgt cctttatgga tgatgcgtct tggtgttttt
    16141 ctctatgatc tcattgctgg accttaccgg attaaacggc ataaggccct gaacgctacg
    16201 gaggttttgc gtctcgaacc gggtcttcaa agaaaaggtc ttaagggggg agtggtttat
    16261 tatgacgctc aaatggacga tgcgagactc tgtcttgaaa atgttctctc ggcttcggag
    16321 gcaggagctc atgccgccaa ctatgcggaa gtgaaggaat atctcaagga gaacggacgt
    16381 gtgatcggtg tcaaggcgcg ggatcacatc ggaaatcgga attttgaggt tcatgccaaa
    16441 aaagttgtgg ttgccgttgg cccctggacc gataaactgc ttaagctgga cgatcttcgc
    16501 gctccccaaa aggtccggac tactcaaggc atccacattg tctacaagga caagctttcc
    16561 ggccgcgcgc tttttgtccc ttctcaaagt gacaaaagga ttttttttgt gatcccgtgg
    16621 cttgggcatt ctttgattgg tacgacagac acggattatg tgggggatcc ggatcgcgtc
    16681 aaggtttcac cggacgatgt ggagtatttg atgcgggaga cccggcgtgt ttttcctgaa
    16741 cttgatatgt caccccggaa aattctttcg actttttcag gactgcgccc cctagtgcgt
    16801 aagggcggtt cggccagcaa ggtttcccgg aaacacctct tttatgaaac accttcaggg
    16861 ctgatttttg tggtaggagg gaaatacacg acttatcgcg cggtcgccga gtcctgcctg
    16921 aagaaaattt ttaaacgaaa aacatggccg ttcgcgctgt gtgacagggg taagtcgcct
    16981 ggaagtatgg acgatgtagc ccgttccttc aaccttgagc cggagacagt tcattcgctc
    17041 ttcgggattt atggcggtcg agtccaggat gtcttggcta tgatagcgaa agagccgagt
    17101 ctgaaggaaa aagtatcctt tgatcctccc gtgatcaagg cgcaattgct ttatgctgtc
    17161 caaaaagaaa tggcgtgcac ggtggaggat attaccgacc gacgtttgtc cctgggctat
    17221 ctcgggcctg tccccgagag cgttcttgcc accatccggg agtttttata aaaaaataaa
    17281 actacttacg gtgtggtcga aaacagaggg ccccctttat gacaatgttt aaagatctga
    17341 aatcttcaag catgaaagcg aaatgatctc gataatgttt aaagtaaaca agggctagag
    17401 tgtgagcttg agttgcaggg tagatttgct aggatttgcc gaagagtaat tgcgcgcaag
    17461 gagatgctac gaaaccagtg gcgatatata aaaatttact ggctggacat tgctatgtaa
    17521 catctcggat gccaagatgg gtaggttggc tggatgagga agtaattctg attgctatcg
    17581 gcatgataca tcaaacagct tcgtgactgt tctgcgattc ttaggcgcta tttgcaactt
    17641 tgtgaatcct gatttgatgt tagtgcagag tatcttggat atcgcgggaa cgatgggtat
    17701 aagaattttc ctaggccaca aaactggatg gcgttttgac gataagattg ctgggatgta
    17761 tgtcttgtag gctactggaa ctcctattat gtagtgtcag cgagcactgg acacgaaggc
    17821 atgaaagtgg gttgcagttt gattgatggt ttgtggggta agaaggaact atcgggggga
    17881 ggcttagttg ttcagaaagg tgcgttaatg aggataatag ttttgtctaa taacccttgg
    17941 tcaaataaca attcctttgg caatacgttc tctaatcttt ttcagggaat gcctgacatt
    18001 aaaattgcca atatatattg caaacatgga ataccgcaga accacattgt agaccatcat
    18061 tttcaaatca cagaaaagat gttaattaaa aattatcttg ctcgaaaaaa tttgtcaggg
    18121 cgtgaaattt ttcaagattg caacgcagaa aggttggagc acaaagaacg tacaattgta
    18181 gattatgcta gaaaaaagcg atggatgatt tttttctggt tgagagaaat tatctgggct
    18241 cttggtcgat ggaaatctcc tgagctggtt agttttatca ctcgttttaa tcccgatatc
    18301 atttttaagc ccttgtacag ttcagtctat atgagcaaaa tcgctttatt tgtaaaaggg
    18361 tataccaaga aaccgatggt ttgctatgcc acggatgatg tctatacatg gcatcagttt
    18421 tccttatctc cgttgtactg gatagaccgg cttataaaaa ggaaatttat tagagatgtt
    18481 gtgaaacaat gcgaaatgct ttatgtgata tctgacgttc aaaaagaaga gtatgggaaa
    18541 tgttttggga aaccatgtaa aaccttaacc aaaggagctg agtttgatag acctctttac
    18601 aaaagttgtc tcaataatcc actgaagttt gtttttacgg gtaacgtcag cgctggtcgt
    18661 tggaagtcgc ttgccctaat aggggattcc ctggagaaaa ttaataaaaa tggaatcagg
    18721 gcaattttgt acatattcac ggaaacgcca ttcagtaaaa agatgctcca cagattacaa
    18781 tcaaggagca tcagggtaat gaacccagtc cccgcgcgtg aaatacagtc catacatgca
    18841 gatgcggatg ttttggtgca tgtggaatct tttagtttga aagaccgact tgtggtgcgc
    18901 cattcttttt ccaccaagct ggtcgactat tttcatgccg ccagatgtgt ctttgcggta
    18961 gcgcctccag atgttgcctc gatgtgttac ttgctaaaaa atgattcggc tgtggttgct
    19021 accagtgaac acgacgtgat aattcagttg aggcatatta tagagaatcc aaaagttgtt
    19081 caccaatacg cccggaaggc ttggagttgt gggaagaaaa atcacctgat aaaaaatata
    19141 caaaaatgtt tgcgtgagga tttgaataat ttagtaatga aggaataaaa tgagatgaag
    19201 gtgcttcaga taaatgcggt tagtggaata cgaagcacgg gtgtatcctg tgttcaactt
    19261 gccgattatt tgaatcaaaa tgggcacgaa ggttttattg cctatggtag cgggttaccg
    19321 tatataaaag gtcacaaaat cggaaatgcg tttgacgcca aggctcatgc tttgctatca
    19381 cgaattacgg gcaaacaagc atatttttcg gttcgggcga cagaaaagtt attgggctac
    19441 atgatacaaa taaaaccgga tgttgttcat cttcgtaacc tgcatagcag ttatgtcaat
    19501 ttaaagcgtt tattgagatt tctttcgcaa caagatatcc ctacggttat tactttgcac
    19561 gattgctggt tctataccgg gaagtgcaca cattatacgg aaaatcggtg tttccggtgg
    19621 aagaaccaat gcggtaattg cccaaggtta aaacgggata accccagttg gttttttgac
    19681 cggacggctc aaatgttaaa ggataaaaaa tcttggttga cgcaaatccc aaggttggct
    19741 gttgttggtg tttccgattg gatagtgagt gaagctcgac aatcattttt atcttctgcc
    19801 agcattgttg cgcggattta taatggaatt gatctggaag tttttagacc acagccagtc
    19861 gattatttga aaaaaaaaca tggtcttgat ggacagtttg ttattttagg cgtggcaagt
    19921 agatggtgtg attccaaagg attgaacaag tttgtcatgt tggctaagac gttacccagg
    19981 gacatgagga ttatt
//
LOCUS       DAOSIO010000007        18820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_3165_length_18820_cov_4.967546, whole genome shotgun sequence.
ACCESSION   DAOSIO010000007 DAOSIO010000000
VERSION     DAOSIO010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 18820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18820
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3165_length_18820_cov_4.967546"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PK590_00650"
     CDS             complement(<1..361)
                     /locus_tag="PK590_00650"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOG23137.1"
                     /translation="MLTIKNLHLKNPVMQSPMAGCTDLAFRVIARRHGLELAMTEMIS
                     AEALVRTHKKTLSLLLRSPDDQPTGCQIVGANPETMAKAAEILEPMGFALIDINCGCP
                     VPKITGQNAGSALMKDPK"
     gene            354..2576
                     /locus_tag="PK590_00655"
     CDS             354..2576
                     /locus_tag="PK590_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23138.1"
                     /translation="MSTFLPLEERLFDPSGERAGKISRRLQVAMELLKRSGIIAHLFS
                     KAKSQIEGGAMNFDEMKSMSGNENWCVIIQSYPSKLKKDLVQRVSEIFSLEKKDAKQA
                     ISNTPLILIDGLSFAMAARIKNFFSRLGATVETTNHDMIKKNCFQVMWPEVPDLSFFL
                     KDEISPSQPARSPKEQQQPVEKKSENPKAKSPFSFLKQTQAEKQEPESPGVESGDGET
                     PRPKSRESVSQNLPLEDISAPVPSGATGEPEVETPAVSRSDEEETPAADPGVWERKAK
                     ELSERLAKIQDGKQKKSGESSAELGEIKSKLKERLMAEHAKSEQEAKDQGSPQESPAE
                     KSANETGDLPELDEQNQALSRKVQELEEELRLKDIELGETDSIRDRCRDLESKIRELE
                     AQREQTMNSVDGELQNLNGQKQSLESKVAELETQLTQARAAQSQFENSSADFSREKSE
                     WAAKIGDLENQIQEKSGQIQERENHCATLQSQVDELTGKVAELNDLAQRRQSEWDEQM
                     RQNESAAQTAKDELARLTRELETERESCQTQSAELNQKIEELETQVRDQQQAVDDAAR
                     AADELKAKEAGLLQRMESSERELGKARDLLNTREQALRERDKILEDLEKKLEDLILEH
                     QEFEKIREENARFNEERADLVQKFEGQLAEQESKLAKMQEDFRRTRSRTERKLTAASR
                     ELGEWVRGVDALRSGLQKLIMFLASDTTSLEPEKRITSARSMSTRSDGASGGAPLQEK
                     "
     gene            2573..3241
                     /locus_tag="PK590_00660"
     CDS             2573..3241
                     /locus_tag="PK590_00660"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HOG23139.1"
                     /translation="MKAVTAEQMRWIDRQSAERFGVDSRQLMENAGRAVADLVAGDFQ
                     PCPVLVVAGKGNNAGDGFVAARYLSRKGFKVEVLLLANPELLSGASAANYQLLSNCPV
                     CFHHAQGTETAQEISSILSPAVLLIDAIWGVGFRGRVEGFYASVIQAMNASGRKIYSI
                     DIPSGLHADTGKVCEVAVKADVTLTLGLPKAGFYLEEGPFYSGDVRIVSIGHPESLLQ
                     PFLN"
     gene            3363..4295
                     /gene="lipA"
                     /locus_tag="PK590_00665"
     CDS             3363..4295
                     /gene="lipA"
                     /locus_tag="PK590_00665"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOG23140.1"
                     /translation="MRYYRRFPDWLRRTLPENSTARTHAILRKFQLNTVCQSAKCPNM
                     QECYSRNTATFMILGKVCTRHCGFCGVESGSLGEVLRSDEPERVAFAAQELGLKYIVI
                     TSVTRDDLDDEGAGHYAATVRAIKAMIPDSRVETLTPDFHASEDLIARVLEAGTDVFN
                     HNLEAVERLQKIARPQADYRRSLTVLETAKRISPDVVTKSGLMLGLGETESEVFEAGR
                     HLLSAGVSVLTLGQYLRPTAHHLEVKEYISPGRFADLANRLEAMGFQKVVAGPYVRSS
                     YRALETYSRLPVENTGCPGRAKHDEILLGGEKNA"
     gene            4288..4614
                     /locus_tag="PK590_00670"
     CDS             4288..4614
                     /locus_tag="PK590_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23141.1"
                     /translation="MREVLAKGLSKRVEDAVIKRWYFEEGDSVTKGDDLLELVSEDKS
                     IVTITVPVTGILAEVFFDKGETVQRDEVLCLIDEEETRLRGDGDDEEDEDGASEPDLD
                     ESEDDF"
     gene            4713..5780
                     /locus_tag="PK590_00675"
     CDS             4713..5780
                     /locus_tag="PK590_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOG23142.1"
                     /translation="MSEILKLKSPAEFIGKQGNGVISFGSGQPDLPPPPEVFQGINLK
                     RDLRYGLIQGELRLREALAHEYPGSTADNFVITNGASEALDLIFRSWGKGGKVLLPRP
                     YYYSYPPIIEMAGMEPVFTDQVEGRIDLEDFKKKVVGCKAMLINSPSNPTGRVESIET
                     LKEIERLTQRLGVYVVSDEVYKDLIYERENYHIQGDHVITVNSFSKTYCMCGVRVGYL
                     WSADKKIVEDVCEQKTHTSMNTNLVGQDMALHAMKTPKDYIVKQQLIWRERRDFIYQG
                     LTDLGFDLWKPEGAFYVLPRCRNARKFLSVLFQEYKVITYLGEWFGAPDRLRFSYALD
                     IKEMEEGLNRIRDAMKKVSVL"
     gene            5815..6624
                     /gene="murI"
                     /locus_tag="PK590_00680"
     CDS             5815..6624
                     /gene="murI"
                     /locus_tag="PK590_00680"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOG23143.1"
                     /translation="MSFTVKSPIGVFDSGLGGLTVVRQIRRILPAEEVLYLGDLAHLP
                     YGTKSESEIRRRSEQCARFLLKRGIKALVIACNSASSVAFARLRKTLPVPVLDVITPA
                     VESAVSATRNYRIGVIATYATIESGSYARILQKKCPDAEFFMKPCPLFVPLAEEGWLD
                     GAIAMQAARKYLLPIKKKSIDTLILGCTHYPLLQGVISKVMGRSVSLIDSAIPTALKL
                     KALLAKRKMSYDGVRQARLKICVTDYVRNFTRIGEAFLGETLECVRRVRIS"
     gene            6621..7304
                     /gene="queC"
                     /locus_tag="PK590_00685"
     CDS             6621..7304
                     /gene="queC"
                     /locus_tag="PK590_00685"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOG23144.1"
                     /translation="MKKAVCLFSGGMDSTVCLYFACREGFAPVAVSFDYGQRHEKEIS
                     SARQITAALQIPLHVIRLAFPWKGSSLLQHDDKVPHGRNPEQMPKKIPTTYVPARNAI
                     FLSLAFSCAEAEKAEVIFFGANAIDYSGYPDCRQEFIAAFAEAMKQGTQTGVEGKPVR
                     IMAPLLHLSKKEIVLLGKELGVPFELTWTCYEGNNRPCGTCDACVLRAKGFYEAGIDD
                     PLVGHAGLF"
     gene            7255..7986
                     /locus_tag="PK590_00690"
     CDS             7255..7986
                     /locus_tag="PK590_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOG23145.1"
                     /translation="MKQGSMIPWLAMPDFSEEKGALISEIFSSIQGEGLHMGERHLFI
                     RFASCNVRCEYCDEAEKPSRIMTVEEVFDEVIRLDQREGPHRFIALTGGEPLLEPEFL
                     KQLIAKLKSKQCRVLLETNGIEWRALTEILESCDSVSMDMKLASVGGHRDYFEEHGRF
                     LAASKGKEMCVKIVVSKDVDLAEFERHLDLIADTNPEIPVFIQSVWQEGFGDGEFLAG
                     LIDRARQRLKDVRLGFQLHKLLHIK"
     gene            8126..8836
                     /gene="rph"
                     /locus_tag="PK590_00695"
     CDS             8126..8836
                     /gene="rph"
                     /locus_tag="PK590_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOG23146.1"
                     /translation="MRIDGRKSNQLRKVDVRKSYLKFADGSCLIEMGDTKVACSASVE
                     DKVPPFCKAEGKGWVTAEYGMLPGSCPERIQREASRGKQSGRTQEIQRLIGRSLRAVV
                     DLKKLGERTIWIDADVIQGDGGTRTASVTGCYIALSLAVKKLLKDKVLAENPLIDSVA
                     AVSVGVVDGIPMLDLCYAEDSKAAVDMNVVMTGSGKLVEVQGTAENRPFSEITLNKLL
                     KLAKNGIRQLSAIQKKNG"
     gene            8829..9494
                     /gene="rdgB"
                     /locus_tag="PK590_00700"
     CDS             8829..9494
                     /gene="rdgB"
                     /locus_tag="PK590_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOG23147.1"
                     /translation="MDKADKPIRLLVATGNQKKLGEFRELFRDFPIQLLSLKDFPGIR
                     EVEETGLTFEENARIKALGYASASGCLTLGEDSGISCDALGGRPGVFSARFSGHEKDD
                     QANNRKLLESLGSVPDDQRMAHYTAAIVLAEPGREIFSTIGEVHGFINREPRGHGGFG
                     YDPIFYYPPFGKTFAEIPIEKKHEVSHRSKALRKTKMFLERYVNDRVRSGDDSREAGN
                     VAY"
     gene            9615..9691
                     /locus_tag="PK590_00705"
     tRNA            9615..9691
                     /locus_tag="PK590_00705"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9649..9651,aa:Val,seq:gac)
     gene            complement(9697..10380)
                     /locus_tag="PK590_00710"
     CDS             complement(9697..10380)
                     /locus_tag="PK590_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOG23148.1"
                     /translation="MSETRPSNNRWLIILVVFLAISSVVQTALLIGQHTKSKSQDKLS
                     VESTSRNIARQTAAPFKSSARQRVLPYTFSSGSQSNDPWEEFDSMSRRINSLMRHAFT
                     FGSPLIAGFSPDSNLNFTPAIDIEETENAYLVKSDLPGLEKDKISISVARGMLTIEGM
                     RETSSEKRNDDTGFYAQERSYGSFYRSVPLPGPVDEANIKADYKNGVLVITLPKSKVS
                     ETPQKVVID"
     gene            complement(10448..10903)
                     /locus_tag="PK590_00715"
     CDS             complement(10448..10903)
                     /locus_tag="PK590_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOG23149.1"
                     /translation="MNHLIRKENQWMDPFDMLADLHGNINRLLSSSLRRGSQENISSF
                     APSLDLREKDDRFVVRADVPGIDKKDIDISVIGNTLMIKGERKSEERQDEKGYYYTER
                     VFGTFHRSVELPVEVDSEKVTASYKDGVLEVTVPKSERAKPKQIKVDIK"
     gene            11234..12961
                     /gene="thrS"
                     /locus_tag="PK590_00720"
     CDS             11234..12961
                     /gene="thrS"
                     /locus_tag="PK590_00720"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOG23150.1"
                     /translation="MSDVNEYEPIYRLRHSASHILAQAVLDLFPGTKLAIGPVIDEGF
                     YYDFDSPVNFQDEDLPKIETRMREIIDSAQSFSERSVDKEEARKFFRDRNEPYKLEII
                     EGIPGDRVNFVQNGPFIDLCGGGHVQNTNEIKAFKLLSVAGAYWRGNENNPMLQRIYG
                     TAFPSQKDLDAYLKQREEAQKRDHRKVGKQLDLFSFHEEGPGLPFWKPKGLILKNLLI
                     AFMRQKLAAYEYVEIETPTILRDKLWRTSGHWDNYRDNMFFLERDEETYAIKPMNCPG
                     GFLMYKEEKHSYRELPLKVAEFGKVHRYERSGVLSGLFRVRGFTQDDAHIFTTPEGLK
                     ASVMETIRFTDEVYKTFGFDYHVELSTRPAKSMGTDEQWQVATDGLRNALEELKMPYK
                     VNEADGAFYGPKIDFHLKDAIGRTLQCGTIQLDMNMPERFDMNYVGEDGGDHRVIMLH
                     RAIFGSLERFIGILIEHYAGAFPVWLSPVQIRVASIAERHAAHAEQIVSRLKQEGFRV
                     DSDFRSEKIGYKVRETELMKIPYLFIVGDKEIENRCVSVRTRGKQDLGAQNLPDMIAR
                     IRQEITDQH"
     gene            12982..13566
                     /gene="infC"
                     /locus_tag="PK590_00725"
     CDS             12982..13566
                     /gene="infC"
                     /locus_tag="PK590_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOG23151.1"
                     /translation="MALDQKKVRANERIRSPQIRLIGPAGEQIGITTVEDGLRRAQDA
                     NLDLVEVASTSTPPVCRIMDLGKYLYSIEKKEKESRKKQKVIDVKEIKMTTKIEDHDY
                     LTKLRNARRFIERGDKVKLTVFFRGREIVYVDKGNQLIARFVQDLSDIAMVERNDGLE
                     GKSIQVYLMKINPAAKKKETPGGSAATAEPTKTE"
     gene            13578..13766
                     /locus_tag="PK590_00730"
     CDS             13578..13766
                     /locus_tag="PK590_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HOG23152.1"
                     /translation="MPKRKTSKTMKKRFRVTKKGKVIGSRTLRRHMMTDRSPKKKRQL
                     RRPLEASKVLAKAIRSRL"
     gene            13812..14162
                     /gene="rplT"
                     /locus_tag="PK590_00735"
     CDS             13812..14162
                     /gene="rplT"
                     /locus_tag="PK590_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOG23153.1"
                     /translation="MPRTTHTVASRRRKKKILKRAKGFVGGRRSLFRTAKETVDRAMA
                     FATRDRKQRKRQFRGLWNARINAACRANGTTYSRLIAALKKANITLNRKSLAEIAARD
                     EKAFLEILKAAQKS"
     gene            14214..15188
                     /gene="pheS"
                     /locus_tag="PK590_00740"
     CDS             14214..15188
                     /gene="pheS"
                     /locus_tag="PK590_00740"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOG23154.1"
                     /translation="MIEEIRKIRESAQAELQGIDSPESLEAFRLLYLGKKGRLPDLFK
                     KFGSIPPEQRKEVGAELNRVKEWIESSVAETRGKLGSSDGVRESFDPTLPGIPPHSGS
                     IHILQQTIEEITTLYARMGFETVEGPEIETEFHNFTALNIPLDHPSRDSFDTFYTDKG
                     QLLRSQTSTVQIRIMQARKPPLRIIAPGRVYRPDTVDASHSFMFHQIEGLMVDEKVTF
                     SDLKGVLHLFLRELFGSEIKIRFRPHFFPFTEPSVEVDIQWGKGWMEILGAGSVDPNV
                     FDAVGYDKSRVRGFAFGLGVERIAMIRHGITDIRHFYENDLRFLRQFA"
     gene            15283..17340
                     /gene="pheT"
                     /locus_tag="PK590_00745"
     CDS             15283..17340
                     /gene="pheT"
                     /locus_tag="PK590_00745"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOG23155.1"
                     /translation="MKISVQWLKDFVDMAPPVLRIADRLTLAGLEVKKVESDGAARDT
                     TFEIEITSNRPDWLSHLGVAREIAAIESLSLKSPSLDPETGRTPPSGWNLDIKDSKGC
                     PYYTGVLIEGISEFKTPDFMARRLLACGIRSINLIVDITNYVLLEYGQPLHAFDADLI
                     RGKEILIRKARAGEKLIAINGATLQLHSEDLVIADSERAVALAGVMGGQETEISERSR
                     NVFLESAFFDPGRVRRTSRRHQISSDSSYRFERRVDPEAVDAGRQRALALIKQYAKPR
                     HISACIKAGEKPRLEVPTLHLRDDEVRDTLGCEVKPAQIASILTRLGCDVKQHAPGSW
                     KVSVPSFRADLQRSIDLVEEVARIFGYNSIPETLPARPPIAVTENPRLNLEKKIRQYL
                     SGAGLHETVTFSLVAEQGLDLVKDLGTGVKIINPLQHGLHWMRPTMITSLLNVIRKNV
                     NAGADSVAIFEIANLYHMPDKGKHPAEDRTCAIALYGKKAAQTWCDAARSLNFYDLKG
                     YVEALIAAAGCQELCVAKIEKSYFSPGRAQEIRAWQTPIAFLGEISSSLLREWDLKAP
                     VFYAEISLENLVKHAQPNRPFVDWPRYPAIERDLSLIVKDDVLSGSLMQEIRGLGEGL
                     IRDVKLFDLFRGKRIPEGYKNLAFRIVYQSPERTLISDQVQKLHDRIAEILVKKYEAT
                     FQK"
     gene            17500..18753
                     /locus_tag="PK590_00750"
     CDS             17500..18753
                     /locus_tag="PK590_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005348865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOG23156.1"
                     /translation="MRPTTLDEILGQGHLLGPGKLLRRTLESDRLTSLILYGPPGTGK
                     TTLALVISRMTSANFVSINAVLSNVAEMRQIQDNARRLLRRSGQRTVLFVDEIHRFNK
                     AQQDILMPDVELGSIILVGATTENPSFSIRGPLLSRSLVCELKTLEENDIIAIMDCAI
                     RDTDRGLGKLSIVLTREAKAHLAKQASGDARRALNALEVGALTTPPDSEGKILFDLKV
                     AEESCQKRMVQYDWDGDFHYDTISAFIKSLRGSDVNAAIYWLAKMIYAGEDPRFIVRR
                     LLIFSSEDIGNADPQALVLTSSAMQALEFVGMPEGRIILSQLVSYLALAPKSNSSIMA
                     IDSALDDVKNQKAESVPTHLRDAHYKGAKKMGHGVGYQYPHNFPDAKVDQVYLPKDAF
                     YYKPRPVGFESELLARLKNLRTKRP"
BASE COUNT         4640 a         4368 c         5354 g         4458 t
ORIGIN      
        1 gtttcgggtc cttcattaag gcggaaccgg cgttctgacc cgtgattttc gggaccggac
       61 agccacagtt gatgtcgatc agggcgaaac ccatcggttc cagaatctcc gcggcctttg
      121 ccatcgtctc gggattggcg ccgacgatct gacaaccggt cggctggtcg tccggtgagc
      181 ggagtaaaag ggagagggtt tttttgtgcg tccggacaag cgcttcggcc gatatcattt
      241 cggtcatcgc gagttccaga ccgtgccggc gggcgatcac ccggaaggcg aggtcggtgc
      301 atcccgccat cggggactgc atgaccgggt ttttcagatg aaggtttttg atcgtgagca
      361 cgtttcttcc tctggaggaa aggttgtttg acccctcggg tgagcgggcc ggaaaaattt
      421 cccgtcgcct tcaggtagct atggaattat taaaacgaag cggtataata gcgcatcttt
      481 tctccaaggc aaaatctcaa attgaaggtg gcgcgatgaa ttttgatgaa atgaaaagta
      541 tgtcaggcaa tgaaaactgg tgtgtcatta tccaatctta tccttccaaa cttaaaaagg
      601 atctggttca gcgggtaagc gaaatttttt ccctggaaaa aaaggacgcc aagcaggcga
      661 tcagcaatac gcctttgatc cttattgacg gcttgtcgtt cgcgatggcg gcgcgtatca
      721 agaacttctt ctcccgactg ggagccacgg tcgagaccac caatcacgat atgataaaaa
      781 agaattgctt ccaggtgatg tggcccgagg tgccggatct ttcttttttc ctgaaagatg
      841 agatctcgcc gtctcaaccg gcccgatctc ccaaagaaca gcaacagccc gttgaaaaga
      901 aatccgaaaa tccgaaagca aaatctccct tttcattttt aaaacagacg caagcggaga
      961 aacaggaacc agaatccccg ggggtggagt ccggtgatgg tgaaacgccc cggccgaaat
     1021 cccgggaatc ggtatcgcaa aacctgcctc tggaagatat ttccgcgccg gtaccgtctg
     1081 gagcaaccgg ggagccggaa gttgaaacgc ctgccgtttc ccgttctgat gaagaagaaa
     1141 ctccggcggc tgatccgggt gtttgggaga gaaaggcgaa agaactgtcc gaacggttgg
     1201 cgaaaattca ggatgggaaa caaaaaaaat cgggcgagtc ttccgcggaa ttaggtgaga
     1261 tcaaatcgaa acttaaagaa cgtttgatgg ccgagcacgc taaaagtgag caggaggcga
     1321 aggatcaggg ctctccgcag gaatctccgg cggagaagtc agcgaatgag accggagatt
     1381 tgccggaact tgatgagcag aaccaggccc tttcgcggaa agtccaggaa ctggaagagg
     1441 agcttcgtct gaaggatatt gaactggggg aaacagacag tatccgtgac cggtgccggg
     1501 acctcgagtc gaaaatcagg gaacttgaag cacaacgtga gcagacgatg aactcggtgg
     1561 atggtgagct ccagaatttg aacggacaaa agcagtcgct tgaaagtaaa gtcgcggaac
     1621 ttgagacaca attgacacag gcacgcgccg ctcaaagcca gttcgaaaat agttccgctg
     1681 atttttcccg ggaaaaatcc gagtgggcgg caaaaatcgg tgatttggaa aatcagatcc
     1741 aagagaaatc cggacagatt caggaaagag aaaatcattg cgcgaccctt cagtcgcaag
     1801 tggacgaatt gaccggcaag gtggcggagt tgaatgatct cgcccagcgg cgtcagtcgg
     1861 agtgggatga gcaaatgcgg caaaacgaat cagcggcgca aaccgccaaa gatgagttgg
     1921 cccggttaac gcgggaactg gaaactgaac gggaatcctg ccagacccag tcagcggagc
     1981 tcaaccaaaa aatagaggaa cttgagacac aagtccggga tcaacagcag gcggtggatg
     2041 atgcggcccg cgcggcagac gaattgaaag cgaaggaagc cggacttctt cagagaatgg
     2101 aaagttcgga acgggagctt ggtaaagccc gggatctttt gaacactcgc gaacaggcgt
     2161 tacgggagag ggacaagatt ctggaggatc tggaaaagaa actggaagat ttgatactcg
     2221 aacatcagga atttgaaaaa atacgggagg aaaacgcccg tttcaacgag gagcgcgcgg
     2281 atcttgtcca aaaatttgaa ggacagcttg ccgagcagga aagtaaactg gccaagatgc
     2341 aagaggattt tagaagaacg cgaagtcgga cggagagaaa gttaacggcc gcgagccggg
     2401 aactgggcga gtgggtgcgg ggggttgatg cccttcgtag cgggcttcag aaattaatca
     2461 tgtttctcgc ttccgacacg accagccttg agccggagaa aaggattacg tcggcgagat
     2521 ctatgtccac gcgatctgac ggcgcttccg ggggcgcgcc gctccaggaa aaatgaaagc
     2581 ggtgacggcc gagcaaatgc ggtggattga ccgccagtcg gcggagcgct ttggtgtgga
     2641 cagtcgccag ttgatggaaa acgcgggccg cgcggtggcg gatcttgtgg cgggtgattt
     2701 tcagccgtgt ccggttcttg ttgtcgccgg aaaaggaaat aatgccggag acggtttcgt
     2761 cgcggcccgg tacttgtctc gaaaaggatt caaggttgag gtgttgcttc tggcaaatcc
     2821 cgaacttctt tccggggctt ctgccgcgaa ttatcagtta ctctcgaatt gccccgtttg
     2881 ttttcaccac gcccagggga ccgaaaccgc tcaggaaatt tcctcgatat tgagtccggc
     2941 tgtcctattg atagacgcca tttggggagt tggcttcagg ggccgggtgg aaggatttta
     3001 cgcttcggtg attcaggcga tgaacgcgtc tggacggaaa atctacagta tcgatattcc
     3061 ttcaggactt cacgccgaca cgggaaaggt ttgtgaggtc gcggtcaagg ccgatgtgac
     3121 cctcacgttg gggcttccca aggcgggttt ttatctggaa gaaggccctt tttattcggg
     3181 ggatgtgcgg attgtttcca tcggtcaccc tgaaagtctt cttcagccgt ttttgaacta
     3241 aacgtagatt tcagaatgag ataggtcgcc ctgaaaacgg ggtatactcc ttgacattaa
     3301 actacctttt tgataaaagt tctcaccttt ttttcgatct ccaccacgag gaaccataaa
     3361 acgtgcgata ctaccgaaga tttcccgact ggctacgccg gacgcttcct gaaaattcga
     3421 ccgctcggac gcacgctatt cttcgaaagt ttcaactgaa cacggtttgt caatccgcga
     3481 agtgccccaa tatgcaggag tgttactcca ggaacacggc cacgtttatg attcttggga
     3541 aagtttgtac gcggcattgt ggtttttgcg gagtggaaag cgggagcctg ggggaagtgc
     3601 ttcgctcgga tgagcccgaa cgcgtcgctt tcgcggccca ggaactcgga cttaaatata
     3661 tcgtgattac ttcggtgacc cgggatgatc tcgacgatga aggcgcgggg cattacgcgg
     3721 ccacggttcg ggcgatcaag gccatgattc cggattcccg tgttgaaaca ttaacgcctg
     3781 attttcacgc cagtgaagat ttgatcgcca gggtactgga ggcgggaacg gatgtgttta
     3841 accataatct ggaagcggtc gaaaggttgc agaaaatcgc gcgaccccag gctgattacc
     3901 gtcgttccct gaccgtcctt gaaacggcga aacggatttc cccggacgtc gtgaccaaaa
     3961 gcggtttgat gctcgggctt ggtgaaacgg aatcggaagt ttttgaggcg ggacggcatt
     4021 tactttccgc cggcgtcagt gttttgacgc tgggacagta tctgaggccg acggctcatc
     4081 atctggaagt gaaggaatac atatcgccgg gaagattcgc ggatctggcg aatcgcctcg
     4141 aggcgatggg ttttcagaaa gtggtggccg gaccttatgt gagaagctct tatcgtgcgt
     4201 tagaaactta tagtcgactt ccggttgaaa atacgggttg tccgggacgc gcgaaacacg
     4261 acgaaatttt gctgggaggt gaaaaaaatg cgtgaagtac tggccaaagg attatcgaaa
     4321 cgagtcgaag acgcggttat caagcgttgg tattttgagg aaggcgattc cgttaccaag
     4381 ggggatgatc tcctagagct ggtgtcggag gacaaatcga ttgtcacgat cacggtgccg
     4441 gtcacgggga ttctcgcgga ggtttttttt gataaagggg aaacggttca gcgtgatgag
     4501 gttctttgtt tgattgatga ggaagaaacg aggctccggg gagatggcga cgatgaggaa
     4561 gatgaggatg gagcctcaga accggatttg gatgaatcgg aagatgattt ttaaaacatt
     4621 ctcaagcgga ggcgaatgaa cgctagcgga gccaggcgcc tgttcgcgaa acgagggggc
     4681 gcccgttttt cgggcggcat ttgatggata cgatgtctga aattcttaag ttaaaaagtc
     4741 ctgccgaatt tatcggtaaa caagggaacg gagtgatcag cttcgggtcc ggccaaccgg
     4801 atcttccgcc gcctcccgag gtttttcagg ggatcaattt aaaaagggat ttgcggtacg
     4861 gcttgattca gggagaactg agactgagag aggctctggc ccatgaatat cccggttcca
     4921 ccgcggacaa ctttgtgatt acgaacggcg cttccgaggc gctggatctt atctttcgga
     4981 gttggggaaa gggtggaaaa gttcttttgc cgcgtcctta ctattattct tatcctccca
     5041 ttattgaaat ggcgggcatg gaaccggttt ttaccgacca ggttgaaggc aggattgatc
     5101 ttgaggattt caagaaaaag gttgtgggat gcaaggcgat gctgattaat tcaccctcca
     5161 atccgacggg acgggtcgaa tcgatcgaga cacttaagga aattgagcgt ctgacccagc
     5221 ggcttggggt ttatgtggtc tccgatgagg tttataaaga cctgatctac gaacgtgaaa
     5281 attatcatat ccagggcgat catgtgatca cggtgaactc tttttccaag acctactgca
     5341 tgtgcggagt tcgcgtgggg tacttgtgga gcgcggacaa gaaaatcgtg gaggatgttt
     5401 gtgagcaaaa gactcacacc tcgatgaata ccaatcttgt gggacaggat atggcccttc
     5461 acgcgatgaa gacccctaaa gactacatcg tgaaacagca actgatctgg cgtgagaggc
     5521 gtgactttat ttatcagggg ttgacggatc tggggtttga tctctggaag ccggaaggtg
     5581 ctttttatgt gttgccgcga tgccgcaatg cccgcaagtt tctgagcgtt ctttttcagg
     5641 aatacaaagt gattacttat cttggggaat ggttcggcgc ccctgatcgg ttgcgtttca
     5701 gctacgccct ggatattaag gaaatggaag aaggactgaa ccggatacgg gacgcgatga
     5761 aaaaagtgag cgttctgtga ttctccggtc gcgatccctc agagaattgc cgccatgtca
     5821 ttcactgtca agagtcccat aggggttttc gattccggtc tcggcgggct tacggtggtt
     5881 cgtcaaatcc gccgaatcct tcccgccgaa gaggtccttt atctagggga tttggcccat
     5941 cttccttacg ggacgaaatc tgagtcggag atacggcgac gctcggaaca gtgcgcccgg
     6001 tttttgctga agcggggaat caaggctctg gtgatcgcgt gtaattcggc ctcgagtgtc
     6061 gccttcgcga ggttgcgaaa gacccttcca gttccggtgc tggacgtgat tacgcccgcc
     6121 gtcgaatccg ccgtatcggc gacccgcaat tatcgaattg gcgtgatcgc gacttacgcg
     6181 acgatagaaa gcggaagtta cgcgagaatc cttcagaaga aatgtcccga cgcggaattt
     6241 ttcatgaaac cttgtccttt gttcgtaccg ctcgcggagg agggttggct ggatggtgcc
     6301 atcgctatgc aggccgcccg aaagtatctt ttaccgatca agaaaaaatc aatcgacacg
     6361 ttaattctcg ggtgcacgca ctatccactg ctccaagggg tcatttccaa ggtgatgggg
     6421 cgttcggttt ccctgatcga ttccgcgata ccgaccgcgc tcaagcttaa ggccctgctc
     6481 gcgaaaagaa agatgagtta cgacggcgtt cggcaagcga ggcttaaaat ctgcgtcacg
     6541 gattatgtca gaaatttcac gaggatcggc gaggcgtttt tgggagaaac gctcgaatgc
     6601 gtgaggaggg tgaggatttc atgaagaaag ctgtttgtct tttttccggc ggcatggatt
     6661 cgacagtgtg cctttatttc gcctgccgcg aagggttcgc gcccgtggct gtttcttttg
     6721 actacggaca gcgtcacgaa aaagaaatat cctcggcgcg gcaaatcacg gccgccctcc
     6781 agatccctct ccacgtgatc cgtctcgcgt tcccctggaa aggaagctct ttgcttcagc
     6841 acgacgataa ggttccccac ggtcgaaatc ctgagcagat gccgaagaaa ataccaacca
     6901 cttatgtccc cgcgcggaat gctatttttt tatcactcgc gttttcctgc gccgaggccg
     6961 aaaaggcaga ggtgattttc tttggggcca acgcgattga ttacagcggc taccccgatt
     7021 gccggcaaga atttatcgcg gcgttcgcgg aggccatgaa gcaggggacg caaaccggcg
     7081 tggaaggaaa accggtccgg attatggcgc cactgctcca cctttcaaaa aaggaaattg
     7141 ttttgctcgg aaaggaactg ggggttccgt ttgagctgac ctggacttgt tacgaaggaa
     7201 ataaccgccc ttgcggcacg tgcgacgctt gtgtgttacg ggccaaggga ttttatgaag
     7261 cagggatcga tgatcccctg gttggccatg ccggactttt ctgaagagaa gggcgccctg
     7321 atcagtgaaa tcttttcttc catccaaggc gaagggttgc acatggggga acggcacctt
     7381 ttcatccgat ttgcgtcctg taatgttcgg tgtgaatact gtgatgaagc cgaaaaacct
     7441 tcgcggatca tgacagtgga agaagttttt gatgaagtga tccgcctgga tcaacgggaa
     7501 gggccgcacc gatttattgc cttgacggga ggagagccgt tgcttgagcc cgagttcctg
     7561 aaacaattga tcgcgaagct caagagcaag cagtgtcgcg ttctcttgga gaccaatggg
     7621 attgaatggc gggcgctgac cgaaattctg gaaagttgcg attcagtgtc catggatatg
     7681 aagcttgcga gcgtcggcgg gcaccgggat tatttcgaag agcatggccg ttttctcgcg
     7741 gcttcaaaag ggaaggagat gtgcgtcaaa attgtcgtat caaaggacgt ggaccttgcg
     7801 gaatttgagc ggcaccttga cctgatcgcg gataccaatc ctgaaattcc ggtttttatt
     7861 caatcggtct ggcaggaagg attcggggat ggggaattct tggcaggatt gatcgaccgg
     7921 gcacggcaga gacttaagga cgtccgcctg ggatttcagt tgcacaaatt gcttcatata
     7981 aaatagtagt tgttaaaacc atttcagcaa aaacttatgc taacctgttt atcccgcttg
     8041 ggctaaacaa gcatcaacaa gtttttgggt tgaccggaaa acggtctcca caaaaactta
     8101 tgctaacctg tttaggggag gggacgtgag aattgacgga agaaaatcca accagctaag
     8161 aaaagttgac gtccggaaaa gttatttgaa attcgcggat ggatcttgtc tgattgaaat
     8221 gggtgatacg aaagttgcgt gttcggcgag cgtggaagac aaagtgccgc ctttctgcaa
     8281 ggccgagggg aaaggctggg tgaccgcgga atacggcatg ttgccggggt cttgtcccga
     8341 gcgtattcag cgtgaagcgt cgagaggaaa acaatcgggg cgaacccagg aaattcagcg
     8401 cctgattggt cgcagtcttc gagcggtcgt tgacctgaaa aaactggggg agcgaacgat
     8461 ctggattgat gccgatgtga tccagggcga tggcggcacg cggacagctt cagtcacggg
     8521 ctgttatatc gcgctgtccc tggcggtcaa aaaattgctg aaagacaagg ttttagccga
     8581 gaaccctctg attgactcgg tggccgctgt tagcgttggg gtagttgacg gaattcccat
     8641 gcttgacctg tgctacgcgg aggattcgaa ggctgcagtg gatatgaacg tggtcatgac
     8701 cggttccgga aagctggtcg aggtccaggg gacggcggaa aatcggccgt tttctgaaat
     8761 tacacttaac aagttactga agcttgccaa aaacgggata cggcaacttt cagcgataca
     8821 gaaaaaaaat ggataaagcg gacaaaccga tccggttgct ggtcgcgaca ggcaatcaaa
     8881 aaaagctggg agagtttcgc gagcttttca gggatttccc aatccagtta ctaagcctca
     8941 aggattttcc ggggatccgg gaagtcgagg aaaccggtct cacgtttgag gaaaacgcga
     9001 gaatcaaggc ccttggatac gcctcggcca gcgggtgcct gacgcttgga gaagattcgg
     9061 gtatttcctg cgacgccctg ggcggacggc ccggcgtttt ttcggcgagg ttttcaggtc
     9121 acgagaaaga cgatcaggcg aacaatcgaa aattactgga atctctcggg agcgtaccgg
     9181 atgatcagcg aatggcgcat tataccgccg cgatcgtcct tgccgaaccc ggccgggaga
     9241 ttttttccac gataggagag gttcacggtt ttattaatcg cgaaccgcgc ggtcacggcg
     9301 ggtttggata cgatccaatt ttttattatc cgccctttgg gaaaaccttc gcggaaatcc
     9361 cgatcgagaa gaagcatgag gtctcgcatc gctcaaaggc tcttcgaaaa acgaagatgt
     9421 ttcttgaacg atacgtgaat gatcgggtcc gaagcggaga cgactcgcga gaggcgggga
     9481 atgtcgcgta ttgacggatg atttttaggt tccgtccagc cacgaacatc ttctcaactc
     9541 tcaccgaata aactcttaac ttgaagtcat cgcgcttatc atctacaatt ctgcctccgc
     9601 gaagatctcc ccgtgggcgg ttagttcagt tggttagaac gctaccttga catggtagaa
     9661 gtcagaggtt cgagtcctct accgcccacc agttttttag tcaataacca ctttctgagg
     9721 cgtctccgat acttttgatt tgggcaaggt gattaccagg acgccatttt tataatcagc
     9781 cttgatattc gcttcgtcca cggggccggg gagggggacg cttctgtaaa aggacccgta
     9841 acttcgctcc tgcgcgtaaa accccgtatc gtcatttctt ttttctgagg aagtttctct
     9901 catcccttcg atggtcaaca tgccccgcgc gaccgagatg ctgatcttat ctttttcaag
     9961 gcccgggagg tcgcttttga cgagataggc gttttccgtt tcttcaatgt caatcgccgg
    10021 agtgaaattg aggttgctgt ccggggaaaa tccagcaatc aggggagaac cgaacgtgaa
    10081 cgcgtggcgc atcaggctgt tgattcgccg ggacatggaa tcaaattctt cccacgggtc
    10141 attgctttga gaaccgcttg agaacgtgta cggtaagacc ctttggcgcg ctgaagattt
    10201 gaagggcgcg gctgtttgac gagcgatatt cctggaagtt gattctacgg atagcttatc
    10261 ctgcgatttg gattttgtgt gttgtccgat cagcagggct gtttggacga ccgatgaaat
    10321 ggccagaaat acaacaagga tgattaacca gcggttgttt gacggacgcg tttcactcat
    10381 gacaaagttc tccctcttgt tttaaaagac tccgccccga ggacaaaatc cagggggcgg
    10441 aatggattta ctttatgtct actttaatct gtttcggttt ggctcgttcc gatttgggta
    10501 ccgtgacttc cagaacgccg tccttatagc tcgcggtgac tttttcagag tcgacctcga
    10561 ccggcagttc aacggagcga tggaacgttc caaagacacg ctcggtgtaa taatacccct
    10621 tttcatcttg acgttcctca ctttttcgtt cgcccttgat catgagtgtg tttccaatga
    10681 ccgagatatc gatatctttt ttgtcgattc ccgggacgtc ggcgcggact acaaagcgat
    10741 cgtccttctc cctcagatcc agactggggg cgaatgagga aatattttct tgcgaacccc
    10801 gtctcaatga tgatgaaagg agccggttaa tgttgccgtg gagatccgcc agcatgtcaa
    10861 acggatccat ccattggttt tctttgcgaa ttaggtgatt catgattgga acctcctttt
    10921 ttgtgttaag tgttggaaat taagaaatcc gataccatag attcaggaat catgccaatt
    10981 tcataaattt cataacttgt ttaaattcaa taagatagaa caaaattatt ttcgcggtaa
    11041 aatgtgccag tgtgaataat tggcacacca taagtgtgcg gaaatttcac tgctaaaaaa
    11101 ataaccagaa agcgatgatt gtcataatcc gataatacat aacgagttag gaaaaaagtt
    11161 gcggcttttg ccgtctgcga gtacaatacg cgaaattttt aacaggaggg gattcttcca
    11221 gattcaatga agcatgtcag acgttaatga gtacgagccg atttaccggc tccggcattc
    11281 tgcctcacat attcttgccc aagcagtcct tgatcttttc cccggaacga agctggcgat
    11341 cggtcccgtg attgatgaag gtttttacta tgactttgac tcgccggtga attttcagga
    11401 tgaggatctt ccgaaaattg aaacaaggat gcgggagatt atcgattccg cgcagtcgtt
    11461 ttcggaacgc tccgtcgata aagaggaagc gagaaagttt ttccgggatc ggaatgagcc
    11521 gtataagctc gagatcatcg aggggattcc gggagaccgg gttaattttg ttcaaaacgg
    11581 tccttttatc gatctctgcg gcggaggcca cgtacaaaat acgaatgaga ttaaagcctt
    11641 taaactgctt tccgtggcgg gagcttattg gcgcggtaac gagaataatc cgatgcttca
    11701 gcgaatttac ggaaccgcgt tcccgagcca gaaggatctg gatgcctacc tcaagcaacg
    11761 tgaggaagcg cagaaacggg atcaccgcaa ggttggcaaa caacttgatc ttttcagttt
    11821 tcatgaggag gggcccggac taccgttttg gaagcccaag ggtttgatct tgaaaaatct
    11881 tttgatcgcc tttatgcgac aaaaactcgc ggcctatgaa tatgtcgaga tcgagacgcc
    11941 gacgattctc cgggataaat tatggagaac atcgggacac tgggacaact atcgggacaa
    12001 catgtttttt ctggaacggg acgaagaaac ttacgcaatc aagccgatga attgtcccgg
    12061 cgggtttttg atgtacaagg aggagaagca ttcttatcgg gaacttcccc tcaaggtggc
    12121 ggagtttggg aaggttcatc gctacgagcg ttcgggagtt ctctccggtc ttttccgggt
    12181 tcgcgggttc acgcaggatg acgcgcatat tttcacgaca cctgagggac tcaaggcgag
    12241 tgtcatggaa accatccgtt ttacggatga ggtatacaaa acctttggtt ttgattatca
    12301 tgtggagctt tcgacgcggc cggctaaatc catgggaacc gacgagcaat ggcaggtcgc
    12361 gacggatggg ctccggaacg ccctggagga actcaagatg ccgtataaag tcaatgaggc
    12421 ggacggggcc ttttacgggc caaagattga ttttcatctc aaggacgcga tcggccggac
    12481 gttgcagtgc ggcaccatcc agctggatat gaatatgccg gagcgttttg acatgaacta
    12541 cgtcggtgaa gatggtgggg atcacagggt gatcatgctt caccgcgcga ttttcggaag
    12601 tcttgagcgt tttatcggaa tattgatcga acattacgcc ggggcgtttc ccgtttggct
    12661 ttcaccggtg cagattcgcg tggcgagcat tgccgagcgt cacgcggccc acgcggagca
    12721 gattgtttcg cggctaaagc aggaaggctt tcgtgtcgat tctgatttcc ggtccgagaa
    12781 gatcggctat aaggtgcgtg aaaccgaatt gatgaaaatc ccgtatcttt ttatcgtggg
    12841 agataaagag attgaaaatc ggtgcgtttc ggtgcgaacc cgtggcaaac aggatcttgg
    12901 cgctcaaaat ttgccggata tgatcgcgcg gatccgccaa gaaatcaccg atcaacatta
    12961 accataacct taggaggtca tttggctctc gatcaaaaaa aagtacgcgc aaacgagcgg
    13021 attcgctcgc cgcagattcg tcttatcggt cccgcgggtg aacagatcgg catcacaacc
    13081 gtggaggatg ggctcaggcg agcgcaggat gcgaacctgg atcttgtgga agtggcttcg
    13141 acttccacgc cgcccgtctg caggattatg gatctcggaa agtaccttta ctccatcgaa
    13201 aagaaggaga aagaatcaag gaaaaagcaa aaggtcattg acgtcaaaga gatcaagatg
    13261 accaccaaga tcgaggatca tgattatttg accaaactca ggaatgcccg ccggtttatt
    13321 gaacgcgggg acaaggtgaa gttgacggtt ttttttcggg gccgcgaaat tgtttatgtg
    13381 gacaagggaa accagttgat cgcgcgtttc gtgcaggacc tttcggatat cgcgatggtt
    13441 gagcgcaatg acggactgga aggaaagtcg atccaggttt atttgatgaa gattaatccg
    13501 gcggccaaga agaaggaaac ccctgggggt tccgcggcga ctgctgaacc aaccaagaca
    13561 gagtgaggat aaaagaaatg ccaaaaagga aaaccagcaa gaccatgaaa aaacgctttc
    13621 gggttaccaa gaagggaaag gtgatcggga gcagaaccct tcggcgtcac atgatgacgg
    13681 atcgaagtcc caagaaaaaa cgccagctcc gtcgccccct tgaagcttcc aaggttttgg
    13741 ccaaagcgat tcggagccga ctttaatacg atcattaaaa gccgtcttaa tcggtaagaa
    13801 aggattgagt catgccaaga acaacgcata cagtcgcaag tcgaagacga aaaaagaaaa
    13861 ttttaaaacg ggcgaaaggt tttgtcggcg gacgacgttc gctttttcga accgcgaaag
    13921 agaccgtcga ccgggcgatg gctttcgcga cccgtgaccg gaaacagcgt aaacgccagt
    13981 tccgcggatt gtggaacgct cgaattaacg cggcgtgccg cgccaatgga acgacctatt
    14041 cccggctgat cgcggcgctg aagaaggcca atatcacgct caatcgcaaa tctctcgccg
    14101 aaattgccgc gcgggatgaa aaagcttttc tggaaattct taaagccgct caaaaatcct
    14161 gaccactgag ttcgtcagtc gcgagggaaa cttcataagg cgaaactttc tttatgatcg
    14221 aagagatccg aaaaatcagg gagtccgcgc aagctgaatt gcaggggatt gattcccctg
    14281 aatccctgga ggctttccgg cttctttatt tggggaaaaa ggggcgtctg ccggatctct
    14341 tcaagaagtt tggaagtatc ccgccggaac aacgcaaaga agtcggcgct gaattgaacc
    14401 gggtcaagga atggattgaa agctcggtcg cggagacgcg cggtaaactg ggaagctcag
    14461 acggcgttcg ggaaagtttt gatcctacct tgcccggaat tccgcctcat tcgggaagca
    14521 ttcacatcct ccagcagacg attgaagaga tcactactct ctatgcccgg atgggtttcg
    14581 aaacggtgga gggtcctgaa attgaaaccg agtttcataa ttttacggcg ctcaatattc
    14641 cgctggatca tccctcgcgc gattcgtttg acactttcta tacggacaag gggcagttgc
    14701 ttcgttccca gacatcgacc gtgcaaattc ggatcatgca ggcccgtaag ccgccgctac
    14761 gaattattgc gccgggtcgc gtgtaccggc ctgacaccgt ggacgcgagc cactcgttta
    14821 tgttccacca gatcgaagga ttgatggtgg atgaaaaagt gactttcagt gacctgaaag
    14881 gcgtgctaca tctttttctg cgcgagcttt ttggctctga aattaaaatc aggtttcgtc
    14941 cgcatttttt cccttttacc gagcccagcg tcgaggtgga tatccagtgg gggaaaggtt
    15001 ggatggaaat tcttggcgct ggtagcgtcg atcctaatgt tttcgatgcg gtgggctatg
    15061 acaagtcgcg cgtgcgaggg ttcgcgtttg gtcttggcgt ggagcgtatc gcgatgattc
    15121 gtcacggtat taccgacatt cgccactttt atgagaatga tttgaggttt ttacggcagt
    15181 ttgcgtgatg aaatttgaag gcgcctgccg gcggggtgcc gttttgttcg cgggactccc
    15241 gattactttc cacgagaaaa cgaaaacaaa tgagtctgtg agatgaaaat atcagtccaa
    15301 tggttaaaag attttgtcga catggcgccg cccgtcctcc ggattgccga tcggttgacc
    15361 ttggctggac ttgaggttaa aaaagtcgag tccgacgggg ccgcgcggga cacgaccttt
    15421 gaaatcgaga ttacatccaa tcgcccggat tggctgtctc atctcggggt cgctcgtgaa
    15481 atagccgcga tagaaagtct ttctctcaag agcccctcgt tggatccgga gaccggacgg
    15541 acgccgcctt ccggctggaa tctggacatc aaagattcga aaggttgtcc ttactacacc
    15601 ggtgttttga tcgaagggat ttcagaattt aaaacgccgg attttatggc ccgaaggctt
    15661 ttggcctgcg gaatccggag tattaacctg attgtcgata ttacgaatta tgttctcctg
    15721 gaatacggcc agcccctgca cgcgtttgac gcggatttga tccggggaaa agaaattttg
    15781 atccggaagg cgagagccgg cgaaaagttg atcgcgatta acggcgcgac gcttcaactt
    15841 cattccgaag atcttgtgat cgcggacagt gaacgggcgg tcgcgctcgc cggagtgatg
    15901 ggagggcaag aaaccgagat cagtgagcgg agccggaatg tttttctgga atcggcgttc
    15961 tttgatcccg gcagagttcg ccggacttcc cgccggcatc agatttcttc tgattcttcg
    16021 tatcgcttcg aacgccgggt ggatcctgaa gccgttgacg cggggcgtca gcgcgcgctg
    16081 gccttgatta agcaatatgc caaacctcgc cacatcagcg cgtgtatcaa ggccggtgaa
    16141 aaaccccggt tggaggtgcc gacccttcac ctccgggatg acgaggtgag agacacgctt
    16201 ggttgcgagg tcaagccggc gcaaatcgcg tcgatcctga cccggcttgg ctgtgatgtc
    16261 aaacaacacg ctcccggaag ttggaaagtc agcgtccctt cgtttcgcgc ggatctgcaa
    16321 cggtcgatcg accttgtgga ggaagtggcg cggatttttg ggtataactc cattccggaa
    16381 acgcttcccg cgaggcctcc gatcgccgtc acggagaatc cacggttgaa cctggagaag
    16441 aaaattcgcc aatacctttc cggcgcggga cttcacgaga cggtgacgtt tagcctcgtg
    16501 gcggagcagg ggcttgatct ggtgaaggac cttgggacgg gggtgaagat tataaatcca
    16561 ttacagcatg ggttgcactg gatgcgtcca acgatgatca ccagcctgtt gaatgtcatc
    16621 cgcaagaacg tgaatgccgg cgcggattcg gtcgccattt tcgagatcgc gaatctttat
    16681 cacatgcctg acaagggaaa gcatcccgcc gaagaccgga cttgcgcgat agcgctttac
    16741 gggaaaaaag cggcgcagac gtggtgtgat gccgcccgat cgcttaattt ttatgatctc
    16801 aaaggttatg tcgaggccct gatcgcggcg gccggttgcc aggagctttg cgtcgccaaa
    16861 atagaaaaaa gttatttttc gccgggacgg gcccaggaga ttcgcgcgtg gcaaaccccg
    16921 atcgcttttc ttggcgaaat ctcgagctca ttactccgtg agtgggacct gaaggctccg
    16981 gttttttacg ccgaaatctc cctcgaaaac ctcgtgaaac atgctcaacc taatcggcct
    17041 tttgttgact ggccgcggta tcccgcgatt gaaagggact tgtctttgat tgtgaaagac
    17101 gatgtgctca gtggatcatt gatgcaggaa atccgggggc tgggcgaggg gttgatccgc
    17161 gacgttaaac tttttgatct ctttcggggg aagcggatcc cggaaggcta taaaaatttg
    17221 gcgtttcgta tcgtttacca gtctcctgaa cggacgttga tctccgatca agttcagaaa
    17281 cttcatgacc ggatcgctga aatcctggtc aaaaaatacg aagcgacttt tcaaaaataa
    17341 ttcttccaac cagtagggcg gagaagtcct atgtctccaa agtctgcggg ggattcgggc
    17401 gatctgtttc atgagccgat ttccgcatct ccgataagta atgaatctga ggccggatca
    17461 aatgaggcct tttcttcgtc ggcgccgctc gccgcgcgga tgcgtcccac cacgcttgac
    17521 gaaattctcg ggcaagggca tttgctgggt cccggaaagt tgcttcgcag gacactcgaa
    17581 tcggatcgct tgacttcgct gattctctac ggcccgcccg gaacgggaaa gacgaccctc
    17641 gcgcttgtta tttcccgcat gacgtccgcg aattttgtct caatcaacgc cgtcctgagt
    17701 aatgtcgcgg aaatgcgcca aattcaggat aatgcccgcc gtctccttcg tcgctccgga
    17761 caaagaaccg ttctttttgt ggatgaaatc catcggttta acaaggctca gcaggatatt
    17821 ttaatgccgg acgttgaact ggggagcatt attctggtcg gggccacgac cgaaaatccg
    17881 tcattctcta ttcgtggccc gttgctgtcc cggtctctgg tgtgtgaact caaaacgctc
    17941 gaggaaaacg atattatcgc catcatggat tgtgccattc gggatacgga tcgcggttta
    18001 ggcaaacttt cgatcgtgct gacgcgggaa gcgaaagcgc acctcgcgaa acaggcctcc
    18061 ggtgacgcgc gccgggccct caacgcgctg gaggtgggcg cgctgacgac gccgccggat
    18121 tctgaaggta aaattctttt tgacctgaaa gtcgcggaag aatcctgcca gaagcggatg
    18181 gtccagtacg actgggacgg agattttcac tacgacacga tcagcgcctt catcaaaagt
    18241 cttcggggat cggatgttaa cgcggcaatt tactggcttg ccaagatgat ttacgcgggg
    18301 gaggatcccc gttttatcgt tcgacggctc ttgatctttt ccagcgagga cattgggaac
    18361 gcggaccccc aggcgctggt tctgacatca agcgcgatgc aggcccttga attcgtggga
    18421 atgcccgaag gaagaattat cctgtctcag ttggtatcct acctcgcgtt agcccccaaa
    18481 agtaattcga gtatcatggc gatcgattca gcccttgacg atgtgaaaaa ccaaaaggct
    18541 gaaagcgtgc cgacccatct ccgggacgct cactataaag gcgctaaaaa gatggggcac
    18601 ggtgtcggtt atcagtaccc gcacaatttc ccagatgcca aggttgatca agtttacctt
    18661 cccaaagatg ccttctacta caagcctcgt ccggtgggtt ttgaaagtga gttgcttgcg
    18721 cgtctaaaaa acctccgaac aaaacggcca taaattatat ttacaggtat ttattgctat
    18781 aaaaagtaat atatatgtta ttaaatccta taaggattat
//
LOCUS       DAOSIO010000008        18520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_3226_length_18520_cov_4.669916, whole genome shotgun sequence.
ACCESSION   DAOSIO010000008 DAOSIO010000000
VERSION     DAOSIO010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 18520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18520
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3226_length_18520_cov_4.669916"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..1935
                     /gene="typA"
                     /locus_tag="PK590_00755"
     CDS             109..1935
                     /gene="typA"
                     /locus_tag="PK590_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOG23157.1"
                     /translation="MPIQKRRKNVRNIAIVAHVDHGKTTLVDALLRQSGAYDFKDGEI
                     AVMDSNPIEKERGITIFSKNASFAYKDTIFNIVDTPGHADFGSEVERILKMVDGVLLL
                     VDAFDGPMPQTKFVLRKSLELHLKPILVVNKVDRPNCRPHEVMDMTFDLFCELNATEE
                     QLDFPIVFASGKDGWAKLNLEDESRDMRPLLDTILRRVPPPIADIEQPFQMLVTMLDY
                     DNYVGRMAIGRVSHGSVKVGEQLALVKRDSPDVLHGRVTKILKYKGMKRVEVDLAEAG
                     DIVTIAGIEEVHVADTLTHPDKPEAIPAVKIDEPTIAMNFACNSSPLAGKDGGRFLTS
                     RHIRERLRREAMMNVGIRLEDVPGEDRVKVSGRGELHLSILIETMRREGYELEVSRPE
                     VIIKEEKGELFEPVEEVVIEVDPGYQGAVIEALGARKAEMKNLMTSAVGTIRMEFLVP
                     SRGILGFRSQLLMMTRGTGIMYQNFYDYQPYKGEIPDRSSGVLISQVPGEAVAYALWG
                     LQERGEIFVRPGDSLYEGMIVGVNNKGHDLVVNAIREKKQTNMRSSGADEAIQLIPPR
                     EMTLEFALEFIEKDELVEITPKNIRLRKRYLSDNDRRRARRN"
     gene            2104..2277
                     /locus_tag="PK590_00760"
     CDS             2104..2277
                     /locus_tag="PK590_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23158.1"
                     /translation="MSNAGRKRTRQSEIAQRQKRRMKIAKLKEKIESARTEGDKEVLL
                     RKLRKVAPCASLQ"
     gene            2295..2564
                     /locus_tag="PK590_00765"
     CDS             2295..2564
                     /locus_tag="PK590_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23159.1"
                     /translation="MKKVIVALFVATLMLGGTSAFAAVDCTKDSPIDQVGDWFATLGK
                     DGMEKNAVLAERKANRVAACAKREAEKAAKKVQQAGSDMKKKMGF"
     gene            2939..3511
                     /locus_tag="PK590_00770"
     CDS             2939..3511
                     /locus_tag="PK590_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOG23160.1"
                     /translation="MREIERELIEKAGKGDTRAFRELYDRTSGYVYGLAYRVTGRKHD
                     AEEVAQDVYVRIHRSLAVFRFQSAFKTWLYRVVLNTALNHLKKKRRIAGHEVEDTEGF
                     NAVASPEAVRSVEISESELKLKRMLERLNPDQRACLVLRELEGMDYQAISETLGINLN
                     TVRSRLKRAREALIAIGKKEVANDAMFQNT"
     gene            3489..3998
                     /locus_tag="PK590_00775"
     CDS             3489..3998
                     /locus_tag="PK590_00775"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOG23161.1"
                     /translation="MRCFKIRERLVTDYRDGELGKREIAQIEEHLRGCEACRELFEEV
                     TRFATLPRPAREIEMEPDSRVWENIDRKISRKRGEARGIRGLLDFITMFFKPHPALKT
                     AVAFSLVVMIGVMIYFPIRTSTPIDLEVSEYLSEQMEMMSILGSGDFERLNSDDDFPI
                     FEADFFDWA"
     gene            4060..4605
                     /locus_tag="PK590_00780"
     CDS             4060..4605
                     /locus_tag="PK590_00780"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOG23162.1"
                     /translation="MNKQWIVKPMVFLAVVSLMMATPLAYGYHGTGEGKQGRSGRFDF
                     KRGGGPECMFKDLGLTQEQQDQLKARRGAEKEQWKTGREALKTKMRALHEEISKPDMD
                     KAKVDAIVMEINTLKGQEFARRTEGIVAMKEILTPEQFVKFQEHHQKKMEKKQWKWKK
                     GPKGGRGDFPQKGDDVSGPAV"
     gene            4720..4959
                     /locus_tag="PK590_00785"
     CDS             4720..4959
                     /locus_tag="PK590_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23163.1"
                     /translation="MIDKPRLKKLLEECIKLETDAIALYAQKIEAPAFFQVFSPEDRE
                     RVQKALAALAEDARSHKGILEAVLAKVQGAEKNEF"
     gene            4949..5488
                     /locus_tag="PK590_00790"
     CDS             4949..5488
                     /locus_tag="PK590_00790"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOG23164.1"
                     /translation="MNSKEEAVAYLQKVLAQERATIHKIQEVISVVKDPQIKSPLEDV
                     FCDEVRHGSFAVLIYSLLQGVAQEEVKAYVFFRQLLDSLLPRPGREKRRYLREPVLGV
                     VRMRSRESALRYEAKCMDFSLSGIGFESDRNVPVNDIYDCEIELYGRPVSLERSGKVI
                     WTKEVLPGTFFGGVQFEGV"
     gene            complement(5508..8207)
                     /locus_tag="PK590_00795"
     CDS             complement(5508..8207)
                     /locus_tag="PK590_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23165.1"
                     /translation="MKILAKLKNWKLAAILSLVYGTASLSFAAAPDSAGSPPQIIPIP
                     DVATPPSGPSADSAALPPKTVLIRGPEKPVSYAEKPYLPVTPDFYLKDRELPLSEPLK
                     DFIRKTLIPKEALYFKPDVGLDEKTGMPYDHIRLRLKEKLLSEVGNYTAASKLSLLIP
                     YLLKIIQEKPELQEAPLSAAEATVFLKKTLLTILDYIGKYPEYGGFLPWVDIRPNATI
                     APASTKLPSLDNGQMTWALAATVAGLEDSPKKDLQEIASLASTILSYQNYRKFYDPEK
                     KLLHGTIQYDPLTQVWSGDKTYYLNDMFEGTMAVLWGVLNGQIPEEAWDNLAIPTTEY
                     MTASREKITTLTGFRSSFQEHWALAYLPFMDSALGPLYQNYLYTQADHARKNRLPGFL
                     STAYDPKGVYRQMGIPAIACNPVDRSDVGVFFATAMGMLISPVVGASWLSEFYQFNDF
                     VTPYGALESVGRDGYADIFTADAKGMTLLAASGGVVEESIKYMQERSVPKKQISMYVK
                     MIELLHAKFKQMLVERNHEPMYLPTQPYPAPTDDTFEVKILPLADPGPVYNVSDHLQS
                     GHLHGKNVRSVGLPTLEQDVAPGKPFAFEYEIPAYFEYFDQWAFRGTYIDRAVSISDM
                     RYLSVTIPTRSDPASFEIELKSDGITLAAAVIDTTKPGILSEDGQYKTLVHTLEPIPE
                     ASYKPFNYIGVALHDPRYLLSKSGAHDRKGTIHLQNISLSKSEISSGAAAVAQQGPEE
                     FEVFRFWRPTHGGLPFHKNEAKSLLHFAGGKGWRGGYIPYTNLSKFNYLYLRVRNTEE
                     GCNCFYIELKHEGDPLIHQFKIPIHVPPDRKWHIFEIAIPEGIQKTFNYFALSDPYAP
                     FDLGAILLSQIPVAIPSIEPVALPETGKKPLECAYRPCVSNET"
     gene            complement(8549..9187)
                     /locus_tag="PK590_00800"
     CDS             complement(8549..9187)
                     /locus_tag="PK590_00800"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOG23166.1"
                     /translation="MGLIKLLYQDPVAFLILATLLLYSVILHEVAHGWVAYLFGDDTA
                     KRSGRLTLAPLPHLNLMGTLALFLIGFGWAQPVPVNYSRLSYQRLGLFCVSFAGCAAN
                     ILIASIAIALLQIETIRALPFLAKSLPILIYINITLGALNLIPIPPLDGSKIVMCFLP
                     EQLNRALSRIEPYGFFILILLIVSGLLTPVIISVQKLIFSAIVSILDFLRLI"
     gene            complement(9343..10764)
                     /locus_tag="PK590_00805"
     CDS             complement(9343..10764)
                     /locus_tag="PK590_00805"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOG23167.1"
                     /translation="MGPVESLRHFIPELILLGGSLTALCLDLFLKGKRYIGWFSVLVL
                     AAASCLLTSPREPLELFYGFFHLDSFANIFRALALIAVAATLLISFGFSPIRKDGQEG
                     EFYGLFLLMAFALITVAAANNLLMIFLTVEFVSILSYLLTGFLKNDPKAKEAAVKYLL
                     FGSFCSGVMLYGMSLLFGAAGSISLPSIGHALNSLPAMKPIATVGLLAFFVGLGFKIS
                     MAPFHLWAPDVYEGAPTPVTAFLTVAPKALGFAVLMRVLSAMFGTSPIESPVILMWLS
                     ILTMTIGNLTAISQKNIKRLLAYSSIAQAGYILMGLAVFPSTLGRDAILVYLFAYLFT
                     NLGAFAIVTFIGEHTGDDEIASYAGLSRRSPVSATLLTVFLLSLTGLPPLAGFIGKYY
                     VFAAAIEANFILLAVAAALNSAVAAYYYFRIIRVMYLVPPTDPQTSLPKPSVSSSVVT
                     WLMFAGVLGLGLFPACFIRLILN"
     gene            complement(10793..12268)
                     /locus_tag="PK590_00810"
     CDS             complement(10793..12268)
                     /locus_tag="PK590_00810"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOG23168.1"
                     /translation="MSSHLLSWIVFTPLLGAFILLCVPKRAAHSIRWIALGTTALTAV
                     LVCLAFPVFRASEPGFQLVERVVWIPQFGVQYLLGVDGISFPMIVLTALISLMACLGS
                     LTHIRVKGGREKEYYFLFLLLETGMMGTFLALDLFLFYIFWEVVLVPMYFLIGIWGGP
                     RKEYAAIKFFLYTLAGSLFMLLGILAVYFAAPIHTFDMTVLANVKFGLLFQKIVFLAF
                     FLGFAIKVPIFPFHTWLPDAHVEAPTPISVILAGVLLKMGTYGFLRFSYPLFPEAAHW
                     FQPAMGILAVIGIIYGGFIALAQTDFKKMVAYSSVSHMGFVLLGLAALNADGFRGALL
                     QMFNHGTITGGLFLLVGVLYDRAHTRELNAFGGLGATMPVYAGILTFFSLASLGLPGL
                     SGFVSEFLTLLGSYQYSKLITGLAAIGILLAAAYLLYMIHRVLLGNLNPKWAKLPDIT
                     ALELSTLVPLMIFILGIGLYPKIILNYMIPTLDALLIAIKI"
     gene            complement(12346..14295)
                     /gene="nuoL"
                     /locus_tag="PK590_00815"
     CDS             complement(12346..14295)
                     /gene="nuoL"
                     /locus_tag="PK590_00815"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOG23169.1"
                     /translation="MVTADWIYLVPLFPLMAFVVNLLWGARFLKNKAAWVSIVASVAA
                     CAVALPICVKVFGGARFSNEFVWLTIGSGTLRFGYLVDPLSASLLSMVTIVGTLIQIY
                     AAGYMAGDPRFSRFFAYVSLFMFAMLTLTIANNFILFFMAWEIMGLCSYLLIGFYFEK
                     TSAAKAGLKAFLTTRVGDVGFLIGMLTLFATLGTLDFSEINSLLTGTSQARLGINTSL
                     LAISALCIFCGTIGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVFLIARSFVI
                     FMAVPGTMPVVTTIGCVTAFMAAFIALTQTDIKRILAYSTISQLGYMVTALGMQGLGA
                     GTFHLITHAFFKALLFLGAGSVIHGTHVQDIRQMGGLFKKMPHTALTFIIASLALAGV
                     PPLSGFWSKDEILLTAFASGHSLVFWTLLLTAFMTAFYMFRLVFMTFFGKARDPKVHA
                     HESPALMTLPLWALAVGAILLGIPGSPMTHHWFQRFLAHAIHHPEYHPSTFVMTCSVV
                     AAISGIALAGIIYSKNIRWAEAIAKTFRPLYQLSFNKFYFDEIYSKYIIRPFNAVGAI
                     FFKFDASIIDGAVNGAGTGSLWIGAVKNWIDQHIVDGLVNFMGTFTRLLNSIVKRLQT
                     GFVQNYLLIAFLSFLVFIIWELKLI"
     gene            complement(14289..14603)
                     /gene="nuoK"
                     /locus_tag="PK590_00820"
     CDS             complement(14289..14603)
                     /gene="nuoK"
                     /locus_tag="PK590_00820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009361911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOG23170.1"
                     /translation="MNLIPLNHILIFSLALFGIGIYGILTRRNIIGILISIELVLNAA
                     NINLIAFSKTTSLSPETGLVFALFVIAIAAITVAVGLAIVLALYRSRKTIFADEVHLL
                     KW"
     gene            complement(14600..15115)
                     /locus_tag="PK590_00825"
     CDS             complement(14600..15115)
                     /locus_tag="PK590_00825"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOG23171.1"
                     /translation="MDLLQLAIKTGIYALMAGTLVFALGVVMLKNLFHAALSLAAVLI
                     GTAILYLVFHAEFMAAVQILLYVGAVITVIIFAIMLTEHIAERSIIQRNKQSLPALLG
                     VLALLVLIGKVLLKTPWPVNPSTLDARVSVSDLGVALMGAYVFPFEVISILLVGALIG
                     AVVIARKDSRP"
     gene            complement(15119..16258)
                     /gene="nuoH"
                     /locus_tag="PK590_00830"
     CDS             complement(15119..16258)
                     /gene="nuoH"
                     /locus_tag="PK590_00830"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOG23172.1"
                     /translation="MDQLFVNLKTWFLNLLGLFLPDWLVMLASIVVSGVVILCLGPFI
                     MMYLTLLERKVIGRIQNRYGPNRVGPWGLLQPVADGIKMFTKEDIIPAYGDKLIHLLA
                     PIVIVVPALMVFLVVPFGRNMTAVDLSIGALYFLAISSITTFPIFMGSWASRNKYSML
                     AGMRTAAQMVSYEIPMTLAIVPVIMMSQSLSTGAIVAAQNGLKWFVFTPWGLVGFLIF
                     FFCAIVECNRPPFDLPEAESELIAGFHTEYSGMKFAFFYMAEYMNAFTLAAIAVTLFL
                     GGWQGPLFPSWLWFFGKTFALIFMMIWFRGTFPRFRVDQLMGFAWKFLLPMTLVNILV
                     TGFWHFNPGAVGWGVSGAVLVGFFFAITALNRPFVPEKRIYELAE"
     gene            complement(16280..16867)
                     /locus_tag="PK590_00835"
     CDS             complement(16280..16867)
                     /locus_tag="PK590_00835"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HOG23173.1"
                     /translation="MKHFFKKGSVPFWGFGILSGLGVTLKNFIDSYFKKPDSGGVTTI
                     QYPEEKSADFEAARNFPFLVYDETPGKPRCVACGICERECPVKCIHVVMAKDEAGKPL
                     RKPAVFDIDYELCMNCGNCEEVCPFHSIFMDHDYEIASYDRSSLLRPMSKLLKSNDYF
                     HKIRPVDAGKTDAVIRAAQEKKAAATKQKDSAGGQ"
     gene            complement(16864..18006)
                     /locus_tag="PK590_00840"
     CDS             complement(16864..18006)
                     /locus_tag="PK590_00840"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOG23174.1"
                     /translation="MTLLDEIKKDGEILEINFGPQHPSTHGVFRMRVLLEGETVRELK
                     PVMGYLHRNQEQIAENTTYAGIIGFTDRMDYINALSNNFSYCLTAEKLASIEVPERAA
                     YIRIIMAELARLINHSAAVGFLLNDLGSFFTPLLYALRERERILDVFEAVTGSRMMCN
                     YFRFGGVKRDLDEDSLAAIKTIVRKFPRFLDEFETLLTSNEILCMRCQNVGVLPADLA
                     VNAGITGPTLRASGVNYDIRKIDRYGLYDRFDFKVPLGKKGDVYDRYYIRILEMRESV
                     KILEQALQALPAGDFIHPKTAPLLRNPRNFKPPVGEAYGRTESPKGELGFYLVSDGTP
                     QPWRCHVRAPSLINLSALEAMCLGQKIADIMIILGSIDIVLGETDR"
     gene            complement(18010..>18520)
                     /locus_tag="PK590_00845"
     CDS             complement(18010..>18520)
                     /locus_tag="PK590_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOG23175.1"
                     /translation="LLLEDPQDLTRVAQYVKESPAFRLDYLSSVTASDFLDYLETIYH
                     FYSMEKKSGPWVLRVRVPRENARVPSLTPLYQSAELQEREAYDLFGILYEGHPDLRRL
                     FLWEGFEGHPLLKDYEQENADTLEARDVEWLEAHGVKVPETMKKNSAELKASGKRALV
                     EKPAEPRQA"
BASE COUNT         4833 a         4365 c         5294 g         4028 t
ORIGIN      
        1 ggggcagcct cctacaggcc tcaagaaatt aactgcctaa agtgggatac acccgggaga
       61 cagtatttcc cttttgatct tgtgtttttc tgttacaatc cacgccttat gccaattcaa
      121 aagcgaagaa aaaacgtacg aaatattgcc atcgtggcgc acgttgacca cgggaaaaca
      181 acgctggtcg acgcgctcct cagacagtcg ggggcctatg atttcaagga cggcgaaatc
      241 gcggtgatgg attctaaccc gattgaaaaa gaacggggga tcacgatttt ctcgaaaaac
      301 gcctcgttcg cttacaaaga cacgatcttt aacatcgttg acacgcccgg ccacgcggat
      361 ttcgggagcg aggtggagcg tatcctcaag atggtggacg gtgtcctgct tcttgtcgac
      421 gcctttgacg gccccatgcc ccagacaaaa tttgtgcttc gaaaatccct ggagctccat
      481 ttaaagccca tccttgtcgt caataaggtc gaccgtccca actgccgtcc ccacgaagtg
      541 atggacatga cgtttgatct tttttgcgaa ttgaacgcga cggaggaaca gcttgatttc
      601 ccgatcgtgt tcgcttcggg gaaagacgga tgggcgaagc tgaatcttga agacgagtct
      661 cgggacatga ggccccttct tgatacaatt ctccgcaggg tgcctccgcc gatcgccgat
      721 attgagcaac ctttccagat gctcgtgacc atgctggact atgataatta tgtgggaagg
      781 atggcgatcg ggagagtttc tcatggctcg gtcaaggtgg gggaacagct cgcgctcgtg
      841 aagcgggact ctcctgacgt tcttcacgga cgggtgacga aaattttgaa atataaaggg
      901 atgaagcgtg ttgaggtgga cctggccgaa gcgggtgata ttgtcacgat cgcggggatt
      961 gaagaagtgc acgtggcgga cacgctgacg catcccgaca agccggaggc cattccggcg
     1021 gtcaagattg atgagccgac gatcgcgatg aatttcgcct gtaactcaag ccctctcgcg
     1081 ggcaaggacg ggggccgttt tctgacctcc cggcatattc gggagagact tcggcgcgaa
     1141 gcgatgatga atgttggaat tcgtctggag gatgtaccgg gagaagaccg tgtcaaggta
     1201 tctggccggg gcgagcttca cctttccatc ttaatagaaa ccatgcgccg cgaaggttac
     1261 gaactcgagg tttcgcggcc ggaggttatc atcaaggaag aaaagggcga actttttgaa
     1321 cctgtcgagg aggttgtcat tgaagtggac ccgggttacc agggcgccgt catcgaagct
     1381 cttggggccc gcaaggctga aatgaaaaac ctcatgacga gcgctgtagg gaccatccgc
     1441 atggagttcc tcgtcccttc gcgcgggatc cttggttttc ggagccaact tctgatgatg
     1501 actcgcggta cgggcattat gtatcagaat ttttatgatt atcagccgta caagggagaa
     1561 attccggacc gctcatccgg agtgcttatt tcccaggtcc cgggggaggc tgtcgcgtat
     1621 gccttgtggg ggctacagga acgcggtgaa atttttgtgc ggccgggaga cagtctttac
     1681 gagggcatga ttgttggcgt gaataacaaa ggtcacgacc tggtcgtgaa cgccattcgc
     1741 gaaaagaagc aaaccaacat gcgttcgtcg ggggcggatg aagcgatcca attgattcct
     1801 ccccgggaaa tgacgcttga attcgcgctt gagtttatcg agaaggatga actggtcgag
     1861 atcacgccaa agaatatcag actccggaaa cgttatttga gtgacaatga ccgtcggcgg
     1921 gcgagacgca actagcctta tgctttggga aaaaacggtt gacctgtcaa tgaggtgaac
     1981 actacaatcc cgtttcaagc ggatgtggct ggattacctc gataaaggaa aagattaaaa
     2041 acttttgtgt ttttgaggta cctggaaaat ctaaaaagta ttaactgaaa gaggagttgt
     2101 gtcatgtcaa acgcaggaag gaaacgcacg cgccagagcg agatcgctca acgtcaaaaa
     2161 cggcgaatga aaatcgcgaa attgaaagaa aaaattgaat cggccaggac cgagggggac
     2221 aaggaagttc ttttgcgcaa gctccggaaa gtggcgcctt gcgcgtcttt acaataacca
     2281 gaaaggatga caaaatgaaa aaagttatcg tggccttgtt tgtggcgact ttgatgttgg
     2341 ggggaacgag cgctttcgcg gcggtcgatt gcacgaaaga tagccccatt gaccaggtgg
     2401 gtgactggtt cgcgacgctg gggaaggatg gaatggagaa aaacgccgtg ttggcggaac
     2461 gtaaggcgaa ccgcgtggcg gcctgcgcca agcgggaagc cgagaaagcc gccaaaaagg
     2521 ttcaacaggc cggcagtgac atgaaaaaga aaatgggttt ctaacgagcc agtatgtcaa
     2581 atcaaaaagg ccggggttat cgccccggcc ttttttttga aaaaacatag cggctggaca
     2641 ccgcgagtag atactgtaac aagtttcaag gcgatgggta atttttttac taccgtaatc
     2701 ctccctccgg aaatgccgcc aggcgtcttt agagttttcg aagattgtag ctctgaaagg
     2761 agattacgac tcgtttcttc tgaaaaccgg atgacatata gcttagctga caagaacaat
     2821 tttgtttcaa gaccctttgt attgttgcca actgtttacg gggcaagagt aaattatttt
     2881 ttgagggaac tttttccccc ggggacgggt ctaacaggag tgagctgagg actggacgat
     2941 gcgggaaatt gaaagagagc ttattgagaa ggctggaaag ggagatacgc gggcgttccg
     3001 ggagctttat gaccggactt ccggttatgt ttacggcttg gcgtaccggg tgacgggccg
     3061 caagcatgac gcggaagagg tcgcgcagga tgtctatgta agaatacatc gctccttggc
     3121 ggttttccgc tttcagtcgg ctttcaagac ctggctttat cgagtggtct tgaacacggc
     3181 tctaaatcat ctcaagaaaa aaagacgtat agcgggacat gaggtggagg acaccgaagg
     3241 ttttaacgcg gtagcttcgc ccgaagcggt ccggagcgtt gaaatatcgg aatcggagct
     3301 gaaattgaag cggatgctgg aacggttgaa tcccgaccag cgggcttgtc tcgtgctacg
     3361 tgaactggaa gggatggatt atcaggcgat ttccgaaaca ttggggatta atctgaatac
     3421 cgtcagatcc cggttgaaaa gggcgcgaga ggctttgatc gcgattggaa aaaaggaggt
     3481 ggcaaacgat gcgatgtttc aaaatacgtg agcgattagt caccgattac agggatggcg
     3541 agcttgggaa acgagagatt gcccagattg aagagcattt gaggggttgc gaagcgtgcc
     3601 gtgaactttt tgaagaagtg acgcggttcg cgaccctccc tcggcccgcg cgagaaattg
     3661 agatggagcc ggattcccgc gtttgggaaa atatcgatcg aaaaatttca cgaaagcggg
     3721 gtgaggcgcg tgggattcga ggtttgctgg attttataac catgtttttt aagccgcatc
     3781 cggctctaaa aaccgcggtt gcgttttctc tggtcgtcat gattggggtc atgatctatt
     3841 ttccgatcag gacgtcgact ccgatcgatc tggaagtttc cgaatattta tcggagcaaa
     3901 tggagatgat gtccatcctc gggagtggag attttgagcg gctaaactct gacgatgact
     3961 ttccgatttt tgaagcggat ttttttgatt gggcctgaag gtgacgggaa cttttcggga
     4021 ctttgcatgg tcttacttgt caaaaaggag gacgtgaaga tgaataaaca atggatcgta
     4081 aaaccaatgg tttttttggc ggtcgtgagt ttgatgatgg cgacacctct cgcctacggc
     4141 tatcacggaa cgggtgaagg caagcaagga agatcggggc gtttcgattt taaaaggggc
     4201 ggtggcccgg aatgtatgtt caaggatctc ggattgaccc aggaacagca ggatcaactg
     4261 aaggcccgcc gtggcgctga aaaagagcag tggaaaacag gccgtgaggc gttgaaaaca
     4321 aaaatgcggg cgcttcacga agagatttca aaacctgata tggacaaggc caaggtcgat
     4381 gccattgtga tggagattaa cactcttaag gggcaggagt ttgcccgtcg gacggaaggg
     4441 attgtggcaa tgaaggaaat tctcacgccg gaacagtttg tcaaattcca ggaacaccat
     4501 caaaaaaaga tggagaagaa acagtggaag tggaagaagg gtccgaaggg aggacggggg
     4561 gattttcccc aaaaaggcga tgatgtttcg ggccctgctg tttgaatgaa aggatgtcgg
     4621 tttccaaaac ccgtgttccg caagtcgcgg agcacgggtt tttcgtgtcg aggcgcccgc
     4681 gctctttacg cccctgaggg ttggtggtag tatttcacca tgattgacaa acccaggctc
     4741 aaaaaacttc tcgaagaatg tatcaaactt gaaacggacg cgatcgcgct ttacgctcaa
     4801 aagattgaag cccccgcttt ttttcaggtt ttctcgcccg aagacagaga gcgcgttcaa
     4861 aaggcgctcg cggctttggc tgaggatgcc cgtagccaca agggaatttt ggaagcggtt
     4921 cttgccaaag tccagggagc cgagaagaat gaattctaaa gaagaggccg tcgcttatct
     4981 gcaaaaagtt ctggcccagg aaagggccac gatccataaa atccaggaag tgatcagcgt
     5041 cgtcaaggac ccgcagatta aaagcccgct agaagatgtt ttctgcgatg aggttcggca
     5101 tggttctttc gcggtgctga tttattccct cttgcaggga gtggcccagg aggaggtcaa
     5161 agcttatgtt tttttccgcc agttacttga ttctcttctt cccaggccag gccgggaaaa
     5221 acgccggtat cttcgcgaac ccgttctcgg ggtggtccgc atgcggagtc gtgaaagcgc
     5281 tcttcgctat gaagcgaaat gcatggattt ttcgctgagt gggatcggat ttgaatcgga
     5341 tcgaaatgtt ccggttaatg atatttatga ttgtgaaata gaactttacg gccgtcccgt
     5401 gtctctggaa cgaagcggca aagtcatctg gacgaaagaa gttttgccgg ggaccttttt
     5461 cggtggcgtt cagttcgagg gtgtgtagtt tgccacggga aaaccggtca tgtctcgtta
     5521 ctcacgcaag gacggtaagc gcattccagc ggttttttcc cggtttcggg caacgcgacc
     5581 ggttcgatgg aagggatggc gaccggtatt tgactgagca ggatagctcc aaggtcaaac
     5641 ggggcgtagg ggtcggacag ggcgaagtag ttaaacgttt tctgaatgcc ttcagggatc
     5701 gcgatttcaa aaatgtgcca ctttcggtcg ggtggaacgt gaatgggaat tttgaattgg
     5761 tgaatcaacg gatctccctc gtgttttaac tctatataga agcaattgca tccttcctcg
     5821 gtatttcgga cacggagata aaggtaatta aactttgaaa gattggtgta aggaatgtat
     5881 ccccccctcc agcccttacc tcccgcgaaa tgaagcaatg acttcgcctc gtttttatgg
     5941 aaggggaggc ctccatgcgt ggggcgccaa aatctaaaaa cttcaaattc ctccggcccc
     6001 tgttgggcca cggccgccgc gccggaagaa atctctgatt ttgaaagaga aatgttttga
     6061 agatgaatcg tccccttccg gtcatgggcc ccggattttg acagcagata gcgcgggtca
     6121 tggagagcga cgccgatata gttgaacggt ttataactcg cctcagggat gggctccagg
     6181 gtgtggacca gagttttgta ttgcccgtct tccgaaagaa tcccgggttt tgtggtatcg
     6241 atcacggcgg cggccagcgt gataccgtcg cttttcagct cgatttcaaa actggctgga
     6301 tcggaacgag tggggatcgt gacgctcaag tagcgcatgt cggaaatgct gacggctcga
     6361 tcaatatagg ttccccggaa ggcccactga tcgaaatatt caaaataggc cgggatttca
     6421 tattcgaagg cgaaaggttt gcccggcgcg acgtcctgtt caagggtggg taatcccacg
     6481 gaacgcacgt tttttccgtg aagatgcccg ctttgcagat ggtccgagac gttataaacg
     6541 gggccgggat ccgccagggg gaggatcttg acttcgaagg tgtcatccgt tggagcggga
     6601 taaggttggg ttggaaggta catcggttca tggtttcgct ccacgagcat ctgtttgaac
     6661 ttggcatgca agagttcgat cattttaacg tacatggaaa tctgtttttt ggggacagac
     6721 cgctcctgca tgtatttaat cgattcttcc acaacgccgc cactcgccgc gagaagggtc
     6781 attcccttgg cgtcggccgt gaagatatcg gcgtaaccgt cacgaccgac cgattccagc
     6841 gctccgtagg gagtgacaaa atcattgaat tgataaaatt cggaaagcca ggacgcgccg
     6901 accacgggag aaattagcat tcccatcgcg gtcgcgaaaa aaactccgac gtcacttcga
     6961 tcaacggggt tgcaggcgat agcgggaatt cccatctggc ggtaaacccc cttggggtcg
     7021 taggccgtac ttaaaaatcc cgggagccgg ttcttccgcg cgtgatccgc ctgcgtgtaa
     7081 agataatttt gataaagtgg tcccagcgcc gaatccatga aaggaagata agccagcgcc
     7141 caatgttcct ggaacgagga gcgaaagccg gtcagggttg tgattttttc ccgggaagcc
     7201 gtcatgtact ccgtcgttgg aatggccaga ttgtcccacg cctcttccgg aatttgtccg
     7261 ttcaggactc cccaaagtac cgccatggtg ccctcgaaca tgtcgttcag gtagtaagtt
     7321 ttatctcctg accatacctg cgtcagcgga tcgtattgga tggtgccgtg gagaagcttt
     7381 ttttcagggt cgtaaaattt ccggtaattc tggtaactca ggatggtgct ggcaagggaa
     7441 gcgatctctt gcaggtcttt tttgggcgag tcttcaagtc cggcgaccgt agccgccaac
     7501 gcccaggtca tttgcccgtt atcaagggac ggaagtttgg ttgacgcggg cgcgatggtg
     7561 gcgttggggc ggatgtcgac ccagggaaga aatccgccgt attcggggta ttttccgatg
     7621 taatccagta tggtcagaag cgtttttttg agaaaaacgg tggcctctgc cgctgaaagc
     7681 ggggcttcct ggagctccgg tttttcctgg attattttta gcaggtaagg gatcagaaga
     7741 gagagtttgg acgccgccgt gtaattgccg acttcactta acaatttttc ttttagacgc
     7801 aggcgaatat ggtcataagg catgccggtt ttttcatcca ggcccacatc gggtttgaag
     7861 tataaggctt ctttcggaat cagggttttg cgaatgaaat ctttcagtgg ctcggaaagt
     7921 ggaagctccc ggtctttcag gtaaaaatcg ggagtgaccg ggaggtaagg tttttcagcg
     7981 taagaaaccg gtttttcggg tccgcgaata agcactgttt ttggaggcaa ggcggcggag
     8041 tcggcggaag ggccagacgg tggagttgct acgtcgggaa tcggaatgat ttgaggcggt
     8101 gatcccgcgg aatcaggcgc ggccgcaaat gacagggaag ctgtcccata gacaagggaa
     8161 agaatggcgg caagcttcca gttttttaat tttgccagaa ttttcatata aattagattt
     8221 tttgatcgtc gatcggcata aataaaagat ccagagctga aattcttcgt tctttgggcc
     8281 cgcgcggtag ggggatcaga cgaaaaacgc ggaattcctt caagattcaa cattttaaaa
     8341 gtcttgagct tgcccgacaa aagaacccat atcttttttc tgaaaatgat cgagtatctt
     8401 ccccaactca aaaacagctg aggggaatat aacccatccg gtattgtttt acaaatccct
     8461 ggtttttact tgctagcact tactatcaac tttatacaat aacgtttatt accttccttt
     8521 tttcgcgtgg attcagtttg tggattctct agatcagacg caaaaaatca aggatcgaaa
     8581 caatcgcgga aaagatcagt ttttgaacac tgataatgac gggagtgaga agcccgctaa
     8641 cgatcagaag gatcaaaatg aaaaatccgt acggttcaat ccgggagagg gcgcggttaa
     8701 gctgttccgg gagaaagcac atcacaattt tggagccgtc cagcggaggg attggaatga
     8761 gattcagggc gccgagcgta atattgatat agatgaggat cggtagcgac ttggcaagaa
     8821 acgggagggc acggattgtt tcaatctgta gcaaggcgat cgcgattgac gcgatcagga
     8881 tattggccgc gcatcccgcg aatgagacgc agaaaagccc aagtctttgg tatgaaagac
     8941 ggctatagtt gaccgggacc ggttgggccc agccaaagcc tatcagaaac agggcgagtg
     9001 tccccatgag attaaggtgt ggcaaaggag cgagcgttag ccgtcccgat cgtttggcgg
     9061 tatcgtcgcc gaaaagatag gcgacccacc cgtgcgcgac ttcatgcaga atgacggagt
     9121 aaagcaagag tgtcgcgaga atcaaaaaag cgaccggatc ctgataaaga agtttgatta
     9181 atcccacgga tttttccttt ccctgaaaat tttaaaattt ttatcgcttc cattatatga
     9241 aaaaagagag gtttttttgg aatattaaat ttccgaattt gacacggatc aatgggggcg
     9301 atatttcttt gactttgaag agaatctggg actttgaaag acttaattaa gaatgagccg
     9361 gataaagcag gcggggaaaa gaccgagacc gaggacgccg gcgaacatga gccaggtaac
     9421 gacggaagag gagacagatg gcttgggaag cgaggtctgg ggatcggtcg ggggcaccag
     9481 gtacatgacc cgaatgattc ggaagtagta gtaggccgcg acggcactgt tcagggcggc
     9541 cgcgacggcc agcaggataa agttcgcttc gatggcggcg gcgaagacgt aatattttcc
     9601 gataaatccc gcgagcggcg gaagtcccgt gagcgaaaga aggaaaaccg ttagtaatgt
     9661 cgcggagacg ggggaacgcc gggacaatcc ggcgtaagag gcgatttcat catctcccgt
     9721 atgttccccg ataaacgtga cgatcgcgaa agccccgaga ttggtaaaaa gataggcgaa
     9781 aagataaacc aaaatcgcgt cgcgccccag cgtggatgga aataccgcga gccccatcag
     9841 gatgtagccc gcctgcgcga tcgaagaata ggcgaggagg cgtttgatgt ttttctgaga
     9901 gatcgcggtg aggtttccga tcgtcatcgt gaggatcgag agccacatca ggatcactgg
     9961 cgattcgatg ggtgacgtcc caaacatggc gctgagcact cgcatcaata ccgcgaatcc
    10021 aagcgctttc ggagcgaccg tcaaaaacgc ggtgaccggt gtcggcgcgc cctcgtaaac
    10081 gtccggcgcc caaaggtgga acggagccat cgagatcttg aagccgaggc caacgaaaaa
    10141 agcgagaagc cccacggtcg cgatcggttt catggcggga aggctgttta aggcatgtcc
    10201 gatcgatggg aggctgatgg aacccgccgc tccgaaaagg agtgacatcc cataaagcat
    10261 gacccccgag cagaaacttc cgaagagtag gtacttaacc gcggcctctt tggccttcgg
    10321 gtcatttttc agaaatcccg tgagcagata agacaggatg gaaacgaatt caaccgtgag
    10381 aaagatcatg agcagattgt tggcggccgc gaccgtgata agcgcgaaag ccatcaaaag
    10441 gaatagtccg taaaactcac cttcttggcc atctttcctg atcggggaaa aaccgaagga
    10501 gatcaacaag gtcgcggcga ccgcgatcag ggccagagcc cggaaaatgt tcgcgaacga
    10561 gtcgaggtgg aaaaagccgt aaaaaagctc aagcggttcc cggggcgaag ttaggagaca
    10621 ggatgccgcc gcgagaacga gaaccgagaa ccagccgata taccgcttcc ccttgagaaa
    10681 caggtccagg cacagagcgg tcaacgagcc tcccagcaag atcagctccg gaataaaatg
    10741 tcgtaaactt tcaacgggtc ccatgacttt tccttttaaa gttcataagc cattatattt
    10801 tgattgcgat taagagcgcg tccagcgtcg gaatcatgta attcaggata attttcggat
    10861 aaagaccaat tccgagaata aagatcatga gcggcacgag cgtggaaagc tcgagcgcgg
    10921 ttatgtccgg aagttttgcc catttggggt tcaaatttcc gagcaagact cgatggatca
    10981 tgtaaaggag atacgcggcg gcgagcagga tcccgatcgc ggccagcccc gtgatgagct
    11041 tcgaatattg ataggatccc agaagcgtta aaaactcgct gacaaagcct gaaagcccgg
    11101 ggaggccgag agaagcgagc gagaaaaacg tcaggatgcc cgcgtagacc ggcatggtcg
    11161 cgccgagccc accaaaagcg tttaactccc gcgtgtgagc gcggtcatag aggacgccga
    11221 caagaagaaa gaggccgccc gtgatggtcc cgtgattaaa catttgaagt agcgcgcccc
    11281 tgaaaccgtc ggcgttcagg gccgcgaggc cgagtaacac aaagcccatg tggctgacgc
    11341 ttgaataggc caccattttc ttaaaatccg tctgtgccag cgcgataaag ccgccataga
    11401 tgatgccaat gaccgcaaga attcccatgg cgggctgaaa ccaatgggcc gcttccggaa
    11461 aaagaggata gctgaaccgg aggaatccgt aggtccccat tttcaggaga acgccggcga
    11521 ggatgaccga gattggggtt ggcgcctcga cgtgcgcgtc ggggagccag gtgtggaacg
    11581 gaaaaatagg gaccttgata gcgaacccga ggaaaaacgc gaggaaaaca attttttgaa
    11641 aaaggagtcc gaatttcacg ttcgcgagta ctgtcatatc aaacgtatgg atgggagcgg
    11701 cgaagtagac ggcaagaatg ccaagtagca tgaagagact tcccgcgagc gtgtacagga
    11761 aaaatttgat cgcggcatat tctttccgcg ggccgcccca gatgccgatc aaaaagtaca
    11821 tcgggaccag caccacttcc cagaaaatgt agaacagaaa aaggtcgagg gcgagaaatg
    11881 tccccatcat gcccgtttca agaagcaaaa acaggaaata atattctttt tcccgtccgc
    11941 ctttgacccg gatgtgcgtg agggaaccga gacaggccat cagcgaaatc agcgccgtga
    12001 gaacgatcat cggaaaactg attccatcca cgccgagaag gtactggacg ccaaattgcg
    12061 gaatccacac gacgcgctcc acgagctgaa aacccggctc cgaggcgcga aaaaccggaa
    12121 aggccagaca gacgagtacg gccgtgagcg cggttgtccc gagcgcgatc caccggatcg
    12181 agtgcgcggc ccgtttgggc acgcagagca gtatgaatgc tccgaggagc ggtgtaaaaa
    12241 cgatccagct tagtaaatgt gaggacattc caatctcctt ttcggtacag agtatggcgc
    12301 gcggcgtccg gtttttcacc gtccgcgaaa cgccctactc cttactcaga taagttttaa
    12361 ttcccaaatg ataaaaacaa gaaaactcaa aaaagcgatc aaaaggtaat tttgaacaaa
    12421 gcccgtctgc agacgcttca cgatcgaatt cagcaggcgc gtgaacgtgc ccataaagtt
    12481 gacgagcccg tccacgatgt gctggtcgat ccagtttttg accgcgccga tccagagact
    12541 gcccgtgccg gcgccgttga ccgcgccgtc gatgatcgac gcgtcaaatt tgaagaagat
    12601 cgctccgacc gcgttgaaag ggcggatgat gtattttgaa taaatttcat caaaataaaa
    12661 cttgttgaat gaaagctgat aaagagggcg gaacgttttg gcgatcgcct ccgcccagcg
    12721 gatgtttttt gagtaaatga ttcccgcgag cgcgatgccg gagattgcgg cgacgaccga
    12781 acaggtcatc acaaacgtgg acggatgata ctcggggtga tggatcgcgt gagcgagaaa
    12841 tcgctgaaac cagtggtgcg tcatgggaga gcccgggatt ccgagcagga tggcccccac
    12901 ggcgagcgcc cagagaggca aggtcatcaa ggccggagat tcgtgagcgt gaactttcgg
    12961 gtctcgcgcc tttccaaaaa aagtcatgaa aacaagccgg aacatgtaaa acgcggtcat
    13021 gaaagctgtc agaagaagcg tccagaaaac aagcgaatgg ccggaagcga acgcggtaag
    13081 aaggatttcg tcctttgacc agaaaccgga aagcggcggt acgcccgcga gcgcgagact
    13141 cgcgatgata aatgtcagag cggtatgtgg catctttttg aaaagaccgc ccatctggcg
    13201 gatgtcctgg acatgagttc cgtggatgac gcttcccgcg ccaaggaaaa gcagggcctt
    13261 gaaaaacgcg tgcgtgatta gatggaatgt tccggcgcca agaccttgca ttccaagggc
    13321 cgtgaccata tagccgagtt gggaaatcgt cgagtaggcc aggatccgtt tgatgtccgt
    13381 ttgcgtgagc gcgatgaagg cggccatgaa cgccgtgaca cagccgatcg tcgtgacgac
    13441 gggcatggtc cccgggacgg ccataaaaat gacgaaggag cgggcgatta aaaaaacgcc
    13501 agccgcgacc atggtggcgg cgtggataag cgcgctcacg ggcgtcggtc cctccatcgc
    13561 gtcaggtaac cacacgtgaa ggggaaattg ggcggatttt ccgatcgttc cgcagaaaat
    13621 acacagggct gatatggcca gcaggctcgt gtttattcca aggcgggcct ggctggttcc
    13681 cgtcaggaga gaattaattt cagaaaaatc cagtgtgccc agcgtggcaa aaagcgtgag
    13741 cattccgatc agaaaaccga cgtctcccac gcgggtcgtt aggaacgctt ttaatccggc
    13801 cttggccgcc gaggtttttt caaaatagaa accgatcagg agatatgaac aaaggcccat
    13861 gatctcccac gccatgaaaa aaagaataaa attattggca atcgtgagag tcagcatggc
    13921 gaacataaaa agggaaacgt aggcgaagaa acggctgaag cgcgggtcgc cggccatata
    13981 gcccgcggcg taaatctgga tcagggttcc gacgatggtg accatggaaa gaagcgacgc
    14041 ggaaagggga tcgacgagat agccaaacct gagcgtaccg cttccgatcg tcagccacac
    14101 aaactcgttt gaaaagcgcg ctccgccaaa aaccttaacg cagatcggaa gagccaccgc
    14161 gcacgcggcg acacttgcca cgatcgaaac ccacgcggcc ttgtttttaa ggaagcgggc
    14221 gccccaaagg agattcacga caaacgccat caacggaaac aaggggacta aatagatcca
    14281 gtcagcggtt accatttcag taaatgaacc tcatcggcaa agatggtttt tcgggagcgg
    14341 tagagcgcga gcacgatggc gagccccaca gcgacggtaa tcgcggcgat cgcgatcaca
    14401 aaaagcgcga acaccagacc tgtttccggt gaaagggatg tggtcttgga aaaggcgatc
    14461 agattgatat tggccgcgtt taaaacaagt tctattgaaa tcaggatccc gataatattc
    14521 cgccgggtca ggatgccgta aatcccgatg ccaaaaagcg cgagtgaaaa aatcaaaatg
    14581 tgattgagcg ggatcagatt cacggccgag aatcctttct cgcgatgacc acggccccga
    14641 tcagcgcccc aactaaaagg atcgagatca cctcaaacgg gaaaacataa gcgcccataa
    14701 gcgcgacgcc gagatccgag accgaaaccc gcgcgtcaag cgttgagggg ttaacgggcc
    14761 acggcgtttt aagaagaacc tttccgatga gaacgagaag cgcgagtacg cctagcaggg
    14821 cgggaaggct ctgcttgttg cgctgaatga ttgagcgttc cgcgatgtgc tcggtgagca
    14881 tgatcgcgaa aatgatgacc gtaatgaccg cgcccacgta gagaagaatc tggacggcgg
    14941 ccatgaattc cgcgtgaaac acgagataga gtatcgcggt tccgatcagg actgccgcga
    15001 gcgaaagcgc cgcgtggaaa agatttttga gcatgacgac tcccagcgcg aacacaagcg
    15061 tgccagccat gagcgcgtaa attcccgttt taatcgccag ttgcaataaa tccatcagtt
    15121 actccgccag ttcataaata cgtttttccg ggacgaaggg acggttcaac gcggtgatcg
    15181 cgaaaaaaaa tcctacgagc accgctccgg aaacgcccca tcccacggct cctggattga
    15241 aatgccaaaa gcccgtgacc aatatgttga ccaacgtcat cggtaaaaga aatttccacg
    15301 cgaaccccat aagctggtcc acccggaaac ggggaaaagt cccccggaac cagatcatca
    15361 taaaaatcag agcgaatgtt ttaccaaaaa accataacca ggaggggaat aacgggccct
    15421 gccagccccc gagaaaaagc gtgaccgcga tcgcggcaag cgtaaacgcg ttcatgtatt
    15481 cggccatata aaagaacgcg aatttcatac cgctatactc cgtgtgaaac cccgcgatga
    15541 gttcggactc cgcttccgga aggtcaaacg gcggacggtt acactcgact atggcgcaga
    15601 agaaaaagat caaaaatccc accagccccc acggcgtgaa cacgaaccat ttcagaccgt
    15661 tctgggccgc gacgatcgcg cccgtggaaa gactttggct catcatgatc acgggaacga
    15721 tcgcgagggt cattggtatt tcgtaggaaa ccatttgggc ggcggtgcgc atgcccgcga
    15781 gcatggagta cttgtttctc gacgcccagc ttcccatgaa aatcgggaac gtcgtaattg
    15841 atgaaatcgc gagaaaataa agcgcgccga tcgaaagatc gaccgccgtc atgttgcgtc
    15901 caaaggggac gacgaggaat accatcaggg ccgggactac gatcacgatc ggcgccaaaa
    15961 ggtggatcag tttgtcgccg taagcgggaa tgatatcttc tttcgtgaac attttaattc
    16021 cgtccgcgac aggttgcaaa agtccccatg gtcccacccg gtttgggccg taacggttct
    16081 gaatgcggcc gatgactttt cgctccagca gggtcaggta catcatgata aacgggccga
    16141 gacagaggat taccaccccg ctgaccacga ttgaggcgag catcacgagc cagtcgggga
    16201 gaaacaatcc cagcagattt aaaaaccaag ttttcagatt tacaaacagt tgatccatgt
    16261 ttctccaata tccaatcgct tattgtccgc cagctgaatc tttttgcttt gtcgccgcgg
    16321 ctttcttttc ctgggccgct ctgataaccg cgtcggtttt tccggcgtct accggacgga
    16381 tcttgtgaaa atagtcgttc gatttcaaaa gtttgctcat gggtctcagg agcgaactac
    16441 ggtcgtagga agcgatctcg taatcatgat ccataaagat cgaatgaaaa ggacaaacct
    16501 cctcgcagtt tccgcaattc atgcaaagct cataatcaat atcaaaaacc gcgggtttcc
    16561 gcaaaggttt tcccgcctcg tccttggcca tcaccacgtg aatgcatttg accgggcact
    16621 cgcgttcgca gattccacaa gcgacgcacc gtggctttcc cggcgtctca tcgtatacca
    16681 ggaacgggaa gtttcttgcc gcctcaaaat ccgcggattt ttcttcaggg tactgaatgg
    16741 tagtaacgcc gcctgagtcc ggttttttga aataagaatc gatgaaattt tttaacgtga
    16801 ctcccaggcc ggagaggatg ccgaaccccc agaaggggac ggagcctttt ttgaaaaaat
    16861 gtttcatcgg tcagtctccc caagaacgat atcgatgctt cccaagatga tcataatgtc
    16921 cgcgattttt tggccgaggc acatggcttc gagtgccgaa agattgatca gtgaaggggc
    16981 ccggacgtgg cagcgccacg gttggggtgt tccgtcactc acgagataaa atccgagttc
    17041 gcccttggga gattcggtcc ggccgtaagc ttctccgact ggcggtttga aattccgggg
    17101 atttcgaagc aagggggcgg ttttggggtg gatgaagtca cccgcgggga gcgcctgaag
    17161 agcttgctcc agaattttca cgctttcccg catttcgaga atgcggatgt agtaacggtc
    17221 gtaaacgtca cctttcttcc caagcggcac cttgaaatca aatcgatcat aaagcccgta
    17281 acggtcgatc ttgcgaatgt catagttgac tcccgaagcg cgaagcgttg gccccgtaat
    17341 gccggcattg accgcgagat ccgccggaag aacgccgacg ttttggcaac gcatgcagag
    17401 gatttcattg gaagtcagaa gcgtctcaaa ctcatcgaga aaccgcggaa atttccggac
    17461 aatggttttg atcgcggcaa gagagtcttc atcgagatcc cttttaacgc cgccgaaacg
    17521 gaaatagtta cacatcatgc gcgagccggt gaccgcctca aagacgtcga gaatccgttc
    17581 ccgttcccgg agggcgtaaa gaagaggggt aaaaaaagaa ccgaggtcgt tcaggagaaa
    17641 accgaccgcg gccgagtgat taatgagcct cgccagctcg gccatgatga tccggatgta
    17701 agcggcgcgc tcgggaacct cgatggacgc gagtttttcg gcggtcaggc aataagagaa
    17761 attgttggaa agggcgttga tgtagtccat ccggtccgta aatccgatga tccccgcgta
    17821 agtggtgttt tcggcgatct gttcctgatt acggtggagg tagcccatga cgggtttcag
    17881 ttcacggaca gtttcgccct ccagcagaac gcgcatgcga aacactccgt gggttgaggg
    17941 atgttgcggc ccgaagttga tctcgagaat ttcaccgtct ttttttattt catccaaaag
    18001 ggtcatattt tatgcctgtc tgggctcggc gggtttttca accagggccc gcttgcccga
    18061 cgccttgagt tccgcggaat tttttttcat cgtttcagga accttgacgc cgtgggcctc
    18121 cagccattcg acgtcccgag cctccaatgt gtccgcgttt tcctgctcgt aatcttttaa
    18181 aagcggatgc ccttcgaatc cttcccagag gaaaaggcgc cggagatccg gatgaccttc
    18241 gtacagaatt ccgaacaggt cgtaggcctc ccgctcctga agctccgcgc tttgataaag
    18301 cggggtcagg gaagggactc gcgcgttttc cctcggaacc cggactcgga ggacccaagg
    18361 gccggatttt ttttccatgg aatagaaatg ataaattgtc tcgagataat ccagaaaatc
    18421 agaggccgtg acgcttgata aatagtccag acgaaaggcc ggagattctt tgacgtactg
    18481 agcgactcgg gtgaggtcct gaggatcctc aagaagaagt
//
LOCUS       DAOSIO010000009        18194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_3298_length_18194_cov_4.056067, whole genome shotgun sequence.
ACCESSION   DAOSIO010000009 DAOSIO010000000
VERSION     DAOSIO010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 18194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18194
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3298_length_18194_cov_4.056067"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            437..7942
                     /locus_tag="PK590_00850"
     CDS             437..7942
                     /locus_tag="PK590_00850"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HOG23176.1"
                     /translation="MRESPSHLAEHTGWRRLEGEDRDRVIAELMDKDQEGVIRYFARW
                     VRATSAMESALWEGWRAFVAGLRFAKIYFNNHHKIYHQQFVTDSEGRIWILKKRRRRV
                     ESETPENDLRVEMLVYLLLKDRVSIAEIHFLTEAEAAQLGIPAGEAGNFFLSRVVLND
                     GSSDEIGEEELRRSFSRIFVADVWLRKVDHMVSNYSFRHAAPVSIDQEEALDYLTLAP
                     NSEGLAWFILAFLLNGIWQQGYTLIAHNWPSLREEMQLKLVLMDMMSQGLKGKIGTVI
                     TTAVDYGLLAGFREAKMLDPASMREAVEEFQSIDRSTILATAREAGYQQREAELVVDY
                     IVAYQKTLASDVRKLWIILNADRVAFEVEKLLSENPENPLSDIREFTTSSRSELRSGG
                     RFKSSEAGRSELREKPFPDPPMGQSPSTQETGYPAFSSRPELWDFQFEMAGHPWSYLL
                     DVLGFRPLDGQTRLVSGVMDVFMDPGRAWILEDLGQLDTEIEDERIAQIVFRNGLPET
                     FRFSDDSVISVGGWIHQFESSVWHGQPLYHFRSVYESHRPESSEFRRHLMEIVKTRKS
                     RSQAVEKILVIGPGMGHEVLTLLEEFPEADFYAADINPLAAANTQANVALAGKLGYKM
                     DKVRVVEGDVFSSPVVKDAGTFDLIVFNAPLLDWDTNVSLEDAAVFSSGLDTFLEECA
                     NYLTSSGAVILGMDNVLTGFQGLRVFYPYGLEVVEEPRITTRTGARSVFHLRKKTSPI
                     ALVRNVDGNSPRSEVRKRGSSTKQSLPDLFPKMLKLMDERRLVVKTHLAWVPAVVVFG
                     SARIPRGHPYYQKAVDLGEAMWRSGFVPRTGAGPSIMEAVLEGYQNTRRLDGVFPGPH
                     NQTQGVKIELPFGTKANDATEQAVFYDRFITRKLALHENIFGTIALPGGFGTLDEVFE
                     VWRRGRPVVLLGKDFWEPVIREWYASWERAGLTARVDLRPTRPYITDDVSKALHYIEE
                     YSNRVVYKTDRYALRRILKNLKHNLIRLRQMPRAVTMVGRPVEGSKELVIAEQLAYRI
                     GEKAKIPVRVASRNLLLHRVFDNAEKHGWLDLLQAVLFYPASDPSKTPAEKGLGKRLI
                     RVNDESNHQFLTTNNSYAFVFFPGGVGTMNKLFDILQIMQTNKGPRKPIILVGRSFWQ
                     PIKDAIVRAMLQSAGQLSLIAPYDPDLLRVVDTVEEAWNELNGYVPVSIGKAENISAI
                     TKDFLRRARAFSGRKIKRLRILSTSGRLVDVIPVPAQVHGKTIDEDVFSDWVVSQVGN
                     LYEGTSGEIYLEKEGADLVLVFKNFVSDLKSNVSGKLDPKKTLKARAETRTSDLNLPL
                     RLQSLAKWNAIGRTQEIRIVKVAADTFFVKTPFVQHEFNLTRTQKFQDSWEVRLWDSG
                     EGRRIGTMVFDEKENQLRIFNVEKEFLPVGAPDILLHYLVEQFPGKDRNRRVGIRLLN
                     NHWLPELGSNLWYLTVNQLQMLWGHPGLEIIENVPEEDSSPGIFSDGLTDEEDVAREK
                     RAEVRVKLDSNLRLSLPADWKNWRSLVGKTVTLVVTGEPGTIEIWLPEKIRGSSDEET
                     RVMMRTMRGRRRLRRSSSRKFPKKISEQGRIHVNVREFFPDLLRDFRPGAELEIQDNG
                     HYIKVTAMPFRENKGTVRSELRYGVKERATNIFRKSIQVQKKEQDVFRSELRAQTAHG
                     RESDGREARLFMDKRQALSDHLVRVLGEAAESGIHKALASLPETVVEGAYSRRFNLEP
                     LFYQKIFRAAWSQDPRHAMIQLSQGLKTAHRFNEEGVVYFDEVVPPDPDSEWRWNEEK
                     SETAFGNVSRKNQRFYLEWAWFPQAMMWLPVQMFYENGAGSKMIKTILSSDRTILKIG
                     TDLARRNLGELGERAGSYRIYSKTPSSGHISIARKKLPSGRRTGNYAFENKSGELCEV
                     WMEQNGVPSRFSFAGERESEELKIFLRKDRTGRVSEYLESHLRINTPLKQLTDELGSF
                     WIQGIRVQAGDRQGVINITKYRAESIEDESREIEYSQSYRVPKEYTGQTAEVFVGEHG
                     VPTLIRIPSQEALPPYEMFLKTLEIRDTESGTGGEILNSFVGDVQASHIRQALAQSAR
                     RMKQGQSGDPFVVVTHVQALVFWEAQGVRGGRRRAEPGLIRRINTGGQTIYVRQTVRR
                     GEFESAPVLAEVFYRIPEVDSGPLRPWKVRLGIYDEKVAVGVPESVFRPFAEYIVEMS
                     GDLPENYASIQEVEISLGVKKLKSDGSDAFDFPKGTSPDSTVRSEQRFPVMEIGVAGR
                     GTLMKKTYKHDSERRVAVDAADGVFRRSSVLGMGKTGLSRALGFGIPAVYANESSKRV
                     TTSSSFLLADPSRLKNAHEQIVVIFDEMPAAGDPVLETLIDVMSKSGFLSFQIVIPGL
                     SMDKRSRFEKDILKRLGKNRQAARAFLNIAVEESRISGFVGRNSENLLVYDMRENGEE
                     ALRRMAPEVTRAGRGKGVLFVYNDDAVALDHKSLALLAAIDALLEKHLPGEIQPASAI
                     LAFVDKMKTQVMSLRAVSSAA"
     gene            8093..>18194
                     /locus_tag="PK590_00855"
     CDS             8093..>18194
                     /locus_tag="PK590_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23177.1"
                     /translation="MTKTSKKILSLITLLCFVFSQSVDAYPGMGIEMRTSRETPSFFQ
                     IQIPSDLATVDGIFEAPARPDPRMILHVQNAHANYDAQQKIKQLLEYLEKTYAIKTIF
                     VEGASEDLNPDYLKFFPDAERNRKLADYLARQGELTGAEMFLLESGAGVRSAERDNGV
                     GARDPLPVRAHGIERAELYRENYEALKKVFGSEAVVSKYLEGFEVRLDKLASNVFLPD
                     LRKILREWKKFENGHREFIPYVKDLVADANRFLGEDLESLFAQVEWPQISRLLVLQMM
                     EKNLDAEKGISEKERVVRFLKEKRVSQNLITGIETFQDQRVTMPLGESAGGTVQPRDL
                     MEQLVMEAGPKGFLFHDYPYFSLYAGYLILKSELDPKGLFDEIGLLFRKILDELAKTD
                     QQKALLDLYRDEELVRKLLNLELTRKNWEEVLARKARLEPEVLVDRLKELGATKEQKR
                     SAWGEDHGTAGEKRAPEPVSRSKKFREIIDIQNAAYAFYDAARRREEVFYEKIDETMR
                     ERRISKAVLVTGGFHTDGITELLREHEISYGILTPRLSEKSDEHLYRSLMLQNQPSTF
                     ELSYLEATSRLISFVGQTKQMNREQVLRNLKPVLKAIGYSGSFKNVRQVIEAFNHSSF
                     SADAGIQIDPEPVRKKGDKPVYRVVLSGTFATKDQILGVIQRLATELPKPTDNPAALA
                     SIAKLAPALAQAGTRTGDVVKTGEVRPPDDKDAEKTVQRIVVAHAKAAGVPTAEYAAA
                     LVREFASHSETGNENHTFDVARPAPRKEARAETRVAEFTYRELLKGFMTMLRSHQRPV
                     HVRGIWPGGGVDPNPNIRNYIQTLRLNRRQKVIPDVIRVYLPITTVEGQLVTGSGRYE
                     DSMVPKSEELLPADVWHALRQASEDGLFELVELGDRAAYDNAARELGVPEQSFFETMV
                     SNNRVLFVSEWVEGTTLDAVLGNTQNPDMHREVGRNLGRVLRELHQNGILVDDNNIDQ
                     YIVTPEGQVLRLDLEKIYGEGAPQEGFEAQYSFFADALRQDYPFFSESFVEGYGENLR
                     ANRSEQRSRIDQVREKLNGMIGIGGWDSIINAVSKEEVEKHLKSLNDGQMPYIGEHID
                     PRLLDIHEKIEILAMREGLSFDEAHKLAREFEQLFRDWLLGPRRGATAALIWPSGEET
                     VIYRMRDMPGDHDILELVVNRKGELYIRRSAWRRDKTTGEWGWQYADFMDHLIQLVGS
                     RIKEDPRFENYQRIYENLMVTVALEKKWGSAVEDLEFISSYPYSELIEIGKKYGIDWT
                     EEEWFPFAADVLFAEAGVDQFMPPVKLAINRAQGEAMRKKGSVPSPAQRPAPILKAMT
                     FDGRTVEVPSSRSESRADTRADAVRSESRASQPDESIYDGMSDLQKEIFNIWKKFADP
                     ALNRIQDAQPVIEVMRAIVDAIREIGEEEFYRELEKTKKHLAKALESVDDYVALWGGP
                     HTSRRWVGLQILEGIKPPKLVTYFEPDKYYFGPRIREIAFAADTIVIADDASYSGKQI
                     RETMEFILKVNPNAKFIIAVPFMSDTAVRKIKSKGTLSQKPSVKILPHNKILTVKEIL
                     NQKNILTQKEFSTPEGVFRNNPGVFGINPDRLLTQSVTLLWYKVSNAVSFYATIVAIV
                     QSVKDVTPPYQEEDSDYYRKETEEYRGFVFGSRPESRQPTGMLAGKPKAESRQEQARS
                     EVRASQPDESIYDGMSESAEEILDKWMGIKSVASALSRIQGAQRVRVIEVMRKIVGAR
                     RVIGKKEFEQELEKTKKHLAKALEPEESKVRHVVLFRGPHTSPRWVWSQIREGIRSLK
                     GETYFEPYEDYFEKGGLIRKIAFSADTFVIADDASYSGEQIRETMEFILKVKPKAKFI
                     IAVPFMTDYAVRKIKSKGTLSQKPSVEILPHKKILILQDILTDNEQKIFSQYSNADIF
                     GKEFVGGKERNLLTTTVTLLWYKIPDGWSFYGLLSQAIQLVRLAQHAQPVIPPYRRQN
                     SKYFSDEEKEYKAFLQKSRSEARVGEETTAPDRAGLGVGLEYCRVHEKALRAEGLDLR
                     ESPLSKGSVEGRDFYDFAARFNTMEEAAKRLADIFIGINRSEEFPDLRLEDPGYWKRY
                     LEWIAGAEEKSRQQLYKIMAESEYRSLRSHQVFWDSYRDHQGALAQEGLQIKETGEVV
                     LTFRGAEKMTATEKAYWISRLFNKHNILGGEFADRSSQRGFGGSVVSRDVLAWVRYFN
                     RQHGYAGVPHGTRRFWEIWRPRDIKEREYFLIESLRSLTPSYPAPFSKKADEAIREAE
                     AGKIKEPIKRKSRVGSPIILSGPPEVATVITTSPASDFLAPGTVLDPRGKPIVPRAPP
                     SGERKISVGIWLAIASGWIVAITLAAILFYSHLFYSQPPQSVSIADSGYHVEFPAEGV
                     LESPHAFDREQLLESGRGEGELPIHISSWVEELSENPSPVSPVIGGPSSFLVYLTYPL
                     KTLTKPSDRPFTRQGVDTHAMRIFTGELGEPSILNVKGGTLPAGFLPLYISPGFTADG
                     SRQSSQPIVMESGQPRDDGEVRFEEVEIIPASFNGKRIPVPDRWVVTDFKVNGQTAPA
                     GQSLDFGTWGITVQPNGIVSTSGSSPHAGTKVEATVREGEGLAKTLNPPGSNEVDDAR
                     VAREIIDIPKEIRGELDNIRARFENGELTREGVAGAVHELMKQYVRYYLDHKQASLDR
                     DETWSNYFRRAITDNGYIVANCDDLATLERIWLLYTGLPPEDVNFVSGILTDPKSPHS
                     RFIGPTQPHSDKTLHATLRIATPGSGSSEIIHEATRGVGVVEENQYEITKSSDLRDHV
                     RDAQEAIRAYKSGDRNPQSWDIGLTDLADALLGEDLSPGQRTTVAKIIAENYLYALGK
                     TGHRPANELSGRILFETANILLRDGELSDELKASLDEIREKIVGWIKEKVSEETPLSS
                     VLKDPGTADAYNLFFNECQADLSADLDSLWSLAMSRVDPWTELSVDAKTAMSALPEDA
                     EGGDSFHVRMLKSFATGEPVNPSDTLEALGREDVSNSVKAELGEKLRATTETGEDMVA
                     LLEAWARLDPNSDQPGEKQLHIEKGLINSFNPVITLETPCVTITGLVDMAKSMSPSNR
                     YMFLGKDPRLAALAAYMTGENIPQNGQELGGYLATQLAKTLSSEAYAYLADLSKPLEG
                     LPVLRFFGMLSGEGNEIRGTIRGNAPLDNLLKKLFNVEKVGISELRFLRETIIREKEP
                     DTETQPSARQEVRTSRSEARAEIRAKQLELFEDVTLGARTLTIGVAQLAKYLEAGILA
                     RQGDGRVDLGHRADSKMRGAVGAVNSGFAVRRQEFSVGGKTFSAGELLIQILDGNIEF
                     VDDRDPGDIYDRAPGGHFAFRGEKRSEVRGSQLAVVQGETLIMPPDDRNLVRLGPWVV
                     SGHYLPAHSGWKLHIQM"
BASE COUNT         4646 a         3982 c         5107 g         4459 t
ORIGIN      
        1 ttctaactgg tcccaacgaa aaaagtctct tcgcatccag attcctccca cgctttttcc
       61 taaaattaaa aatgaagatt atgcggtcct tttttccccc acgggttcgg aggtttttta
      121 tcttctcccg ggaagcgaca ttgatgctct cgcccggggc gatagaaaaa gatttggtga
      181 gttctccagt tatgaggaat ggctgaattt tgcaagttgg gttggaagcc agagccggca
      241 agtcgtcttg ataaatcgtt caggagagat ttccgttgag gaaaaatggc ttcgagaggc
      301 ccatattaac ccccgtcgac ttgctatggg atctcaaggg gacttacttg ttttatttga
      361 tgggcagaat taccggaatg ccattaacgc gccagacttt cgaccgcaag atttgggtcc
      421 agtcgccaga tccgaaatgc gcgaaagtcc cagccacctg gctgaacata ccggatggcg
      481 tcgtcttgaa ggggaagatc gggaccgggt tatcgcggaa ctgatggata aggaccagga
      541 aggcgtcatt cgttattttg cgagatgggt tcgcgcgact tcagcgatgg agtctgccct
      601 gtgggaaggg tggcgtgcct ttgtcgcggg tttgaggttc gccaagattt attttaataa
      661 tcatcacaaa atctatcacc agcaatttgt aacggactcc gagggcagga tctggatact
      721 taaaaaaaga aggcgccgcg ttgaatccga gacaccagag aatgatctcc gggtggaaat
      781 gctggtctac ctgctcctga aagacagggt cagcatcgcc gaaatccatt ttttgacgga
      841 ggcggaagcc gctcaactgg gtattccggc cggggaagcc ggaaattttt tcctgtcgcg
      901 agtcgtttta aatgacggct cctccgacga aattggcgag gaagaactcc gcaggagttt
      961 ttccagaatt tttgtcgcgg acgtgtggct ccggaaagtg gatcacatgg tttcgaatta
     1021 tagttttcgc catgcggcgc cggtttcgat cgatcaagaa gaagctctgg attatctgac
     1081 gctggccccg aattctgaag gattggcctg gttcatcctt gcgtttcttt taaacggtat
     1141 ttggcaacag ggttacaccc ttatcgcgca taactggcca agtcttagag aggaaatgca
     1201 actgaagctg gttctgatgg atatgatgag ccaaggcttg aaaggaaaaa tcgggacggt
     1261 tattacgacc gcggttgact atggcctgct ggccgggttt cgggaggcca agatgctgga
     1321 tccggccagt atgagggaag ccgttgagga atttcaatcg attgaccggt cgacgatcct
     1381 tgcgaccgcc cgggaggcgg ggtatcagca acgggaagca gaattggttg tcgattatat
     1441 tgtcgcgtat cagaaaacgt tggcctcgga cgtgagaaag ctctggatta tcctaaacgc
     1501 tgaccgggtt gctttcgagg tggagaaact tctgagcgag aaccccgaga atcccctttc
     1561 cgacatcagg gaatttacga catcgtcccg ttccgagttg cgatcggggg gcagattcaa
     1621 atcttccgaa gcgggacgtt cggaattacg tgaaaagccg tttcctgacc cacccatggg
     1681 acagagccct tcaactcaag aaacggggta tcccgctttt tcttctcgcc ccgaattatg
     1741 ggattttcag tttgaaatgg cagggcatcc ctggagttat ctgcttgatg tcttggggtt
     1801 cagacctctt gatggtcaaa ccagattagt ttcaggcgtg atggatgtgt tcatggatcc
     1861 tgggagggcc tggattttgg aagacctggg acagctggat accgaaatcg aagatgaaag
     1921 gattgcccaa attgttttta gaaatggcct tcccgagaca ttcaggtttt cggatgattc
     1981 tgttatttct gttggaggct ggatccatca atttgaatca tcagtgtggc atggtcagcc
     2041 gctttaccat tttcggtcag tctatgaatc tcacaggccc gagtcttcgg aatttagacg
     2101 tcatctcatg gaaattgtaa aaacccgaaa gtcccgttcg caagcggtgg agaaaattct
     2161 cgtgatcgga cccggcatgg ggcatgaggt attgacgctt ctggaggagt ttccggaagc
     2221 tgatttttat gcggctgata tcaatccgct cgcggctgct aacacccagg caaacgtggc
     2281 gttggcgggg aaacttggat acaagatgga taaggtgagg gtggttgagg gggatgtgtt
     2341 ctcttctccg gttgtgaagg atgcggggac ctttgatctg attgttttta acgcgcccct
     2401 gctggattgg gacaccaacg tttcactgga ggatgcggcg gtatttagtt cggggctgga
     2461 tacgttcctt gaagaatgcg cgaattattt aacgagttcc ggcgctgtga ttttggggat
     2521 ggacaatgtg ctgaccggat tccagggatt gcgcgttttt tatccgtatg gtctggaggt
     2581 agttgaagaa ccccggatca cgacccgtac gggagctcgg tcagtttttc acttgagaaa
     2641 aaaaacctct cccattgcgc ttgtccgaaa cgtggatgga aattcccctc ggtcggaggt
     2701 gaggaaaagg ggttcatcaa cgaaacaatc gttgccggat ttattcccga agatgttgaa
     2761 attgatggat gaacgccggc ttgtggttaa aacccatctt gcctgggtgc cggccgtggt
     2821 cgtgtttgga agtgcgcgaa ttccccgagg gcatccgtat taccaaaagg cggtagatct
     2881 cggggaagcg atgtggcgct ccgggtttgt gcccaggacc ggcgctggcc ccagtatcat
     2941 ggaagcagtt cttgaagggt accagaatac ccgccggtta gacggagttt ttcccggtcc
     3001 ccataatcaa acgcagggag tcaagattga actgccgttt gggacaaaag cgaacgatgc
     3061 gactgaacaa gcagtttttt atgatcggtt tatcacgcga aaacttgctc ttcatgaaaa
     3121 tatattcggc accattgctt tgcccggggg atttggaacg cttgatgaag tgttcgaggt
     3181 gtggcgtcgc ggccggccgg tggtgctatt gggcaaggat ttttgggagc ctgtgatccg
     3241 ggaatggtac gcgagttggg aaagggccgg gttgacggca cgtgtggatc ttcggcctac
     3301 gcgtccttat atcaccgatg atgtctccaa ggcgctccac tatattgagg agtattcgaa
     3361 cagggtggtt tataaaacgg atcggtatgc cttgcggcgg attctgaaaa acctgaagca
     3421 taatctgatc cgtttgcggc aaatgccccg cgcggttacc atggtgggtc gtccggtgga
     3481 aggaagcaaa gaacttgtga ttgcggaaca gcttgcctac cggattgggg aaaaggcgaa
     3541 gattcccgtt cgagtggcaa gccgcaatct gcttctacac cgcgtttttg acaatgccga
     3601 gaaacatgga tggctggatt tactccaagc ggtccttttt tatccggcga gtgatccttc
     3661 caaaacaccc gcggaaaaag gtttgggaaa acggttgatc cgggtcaatg atgaatcgaa
     3721 tcatcagttt ttgaccacca acaactccta tgctttcgtg tttttccccg ggggagtggg
     3781 gaccatgaac aaactttttg atatcctcca gattatgcaa accaataaag gcccccgcaa
     3841 accgatcatt ctggtcgggc gttctttttg gcaaccgatc aaggacgcga ttgtccgggc
     3901 catgcttcag tcggcaggcc agctttctct catcgctcct tatgaccctg atctgttgcg
     3961 tgttgtggat actgtcgagg aagcctggaa tgaacttaac ggctatgtcc cggtttcgat
     4021 tggcaaagct gagaatattt ccgcgataac aaaagatttc ctgagaaggg cccgtgcttt
     4081 ttcaggccga aagatcaaga gacttcggat tttatcaact tcaggacgcc tggtggatgt
     4141 tattccggtg ccggcgcaag tccatggaaa aacgattgac gaagatgttt ttagtgactg
     4201 ggtcgtcagc caggtgggca atctttatga ggggacatcc ggcgagatat acctggaaaa
     4261 agaaggcgcg gatctcgtcc tagtatttaa aaattttgtt tctgatttga agtcaaatgt
     4321 ttccggaaag ttggatccca aaaaaacatt gaaggcccgc gctgaaacaa gaacctccga
     4381 tcttaacctt cccctaaggc tccaatcgct tgcaaagtgg aatgcaatag gccggacgca
     4441 ggaaatccgg attgtgaaag tagccgccga cacttttttt gtgaaaactc catttgtaca
     4501 acacgaattt aatttaaccc ggacccaaaa gtttcaggat agctgggagg tcaggctttg
     4561 ggatagcggt gaggggcgac ggatcggaac aatggttttt gacgagaaag aaaaccagct
     4621 tcgtattttt aatgttgaaa aggagttttt accggtcgga gcgcctgata ttttactgca
     4681 ttatctagtg gaacaatttc cgggaaaaga caggaaccgt cgagtgggga ttcgcctttt
     4741 gaataatcac tggcttccgg aattaggatc gaatttgtgg tatctcacgg tgaatcagct
     4801 ccaaatgtta tgggggcatc ccggtttgga aattattgaa aacgtcccgg aggaagattc
     4861 gagccccgga attttttcgg atgggctgac ggatgaagaa gacgttgctc gtgaaaaacg
     4921 ggccgaggta agggttaaac tggattccaa ccttcgtctc agcctcccgg cggactggaa
     4981 gaactggcga agcctggtcg gaaagacggt gacgcttgtt gttaccggag aaccgggcac
     5041 gatagagatt tggttacctg aaaagatccg gggttcctct gacgaagaaa cacgtgtgat
     5101 gatgcgaaca atgcgaggac ggcgtcggct acggcggtcc agctcgcgca agttccctaa
     5161 aaagataagc gagcagggga gaattcacgt taatgtccgt gagttttttc cggacctgct
     5221 ccgggatttt cggccggggg ctgaacttga gatccaggat aacggtcatt atattaaagt
     5281 gacggccatg ccgtttcgcg aaaataaagg gacggtccgc tccgaactgc gttacggggt
     5341 gaaggagcgc gccacgaata tttttagaaa atcgatacag gttcagaaaa aggaacagga
     5401 cgtgttccgg tcggagcttc gggcgcaaac agcccatggg cgggagagtg acgggcgtga
     5461 ggcaaggctg tttatggata aaaggcaggc gctttcggac catctggtca gagttttggg
     5521 tgaagccgcc gaatccggca ttcataaggc gcttgcttct ctccccgaga cggtggtgga
     5581 aggcgcgtat tcacgccgtt tcaatctgga accgttattc taccaaaaga tttttcgtgc
     5641 cgcgtggagc caggatcccc gccacgcgat gatccaattg tctcagggat taaaaacggc
     5701 ccatcgtttt aacgaggaag gggtcgttta ttttgatgaa gtagttccgc ctgatcctga
     5761 cagtgagtgg aggtggaatg aagaaaaatc ggaaactgcc tttggaaatg tgtctcgcaa
     5821 aaatcagcgt ttttatctcg aatgggcgtg gttcccccag gcgatgatgt ggctccccgt
     5881 acagatgttt tatgaaaatg gcgccggatc caaaatgatc aaaaccattt tatcttcgga
     5941 ccggacgatc cttaaaatcg ggaccgacct agcccggcga aatctgggtg aactgggaga
     6001 gcgcgccgga agctaccgga tctactccaa aacaccttcg tccgggcata tttccatagc
     6061 ccggaagaaa ctgccatcgg ggagaaggac ggggaattat gcatttgaga acaagagcgg
     6121 ggagctctgt gaggtttgga tggagcaaaa cggtgtcccc agccgtttca gttttgcggg
     6181 agagcgggaa tcggaagaat taaagatctt cttaaggaag gaccgaaccg gacgtgtttc
     6241 ggaatatctg gaatcacacc taagaatcaa cacaccgctt aagcaactga ctgacgaatt
     6301 aggaagtttc tggatccagg ggattcgtgt tcaagccggg gatagacaag gcgttatcaa
     6361 tataacgaag tatcgcgcgg aatcgatcga agatgaatct cgggaaatcg aatattcaca
     6421 atcttacagg gttcccaaag aatataccgg acaaaccgcg gaagtttttg tcggggagca
     6481 tggggtgccg actctgatcc ggatcccgtc ccaggaagcc cttccgccct atgagatgtt
     6541 tctcaagacg cttgaaattc gtgacactga aagcggaacc ggaggtgaaa tcctcaactc
     6601 gttcgtcgga gatgttcagg cttcgcatat caggcaggcg ctggctcaat cggcccggag
     6661 aatgaaacaa ggtcaatcgg gagatccgtt tgtggtggtg acgcacgtcc aggctctggt
     6721 tttttgggaa gcccaggggg ttcggggagg tcgacgcagg gcggagccgg gtttgatccg
     6781 caggataaat acggggggac agacgattta tgtccgccaa actgtccggc gcggtgaatt
     6841 tgaatcggcg cctgttttgg ccgaggtttt ttaccgaatt ccggaagtag attccggccc
     6901 gttacgtccg tggaaagttc gtctgggtat ctatgatgaa aaagttgcgg tgggggttcc
     6961 tgaatctgtt tttcggccat ttgccgagta cattgtcgaa atgtccgggg atttaccgga
     7021 aaattatgcg tcaatacaag aagttgaaat ctcccttggg gtaaaaaaat taaaaagtga
     7081 tggttctgat gcttttgatt ttccaaaggg gacttctccg gattccacgg ttcgttccga
     7141 acagcgcttt ccggtcatgg aaattggtgt ggctgggcgc gggacgttga tgaagaagac
     7201 atataaacat gattcggagc ggagggttgc ggttgacgcg gcagatggag tgtttcgccg
     7261 ttcgagtgtg cttgggatgg ggaaaacagg gctttcaaga gccctgggtt ttggaattcc
     7321 ggcggtctac gcgaatgaga gttcgaaacg cgtgacgact tcctcctcat tcctgttggc
     7381 tgatccttca aggctgaaaa atgcccatga gcagattgtg gtgatttttg acgagatgcc
     7441 cgcggcgggt gatcctgttt tggaaacgtt aatagacgtc atgtcgaaaa gcgggttttt
     7501 atcgttccag atcgtgatac ccggtctttc catggataaa cgttcccgtt tcgaaaagga
     7561 tattttgaaa cggctgggaa agaaccggca ggccgcgcgc gccttcctta atattgccgt
     7621 tgaggaatcc aggatatccg gatttgtggg acgtaatagc gaaaatcttc tggtttatga
     7681 catgagggaa aatggtgagg aggctttgcg gcggatggcg ccggaggtca cgcgggctgg
     7741 ccgggggaag ggagttcttt ttgtgtacaa tgatgacgcg gtggctctcg accataagtc
     7801 tttggcgctt ctcgcggcga ttgacgctct tttggagaag catcttcccg gggagatcca
     7861 acccgcgagt gcaatcctcg cctttgttga taagatgaag acccaggtaa tgtctctccg
     7921 ggctgtttcc tccgcggcct aaaaatcata aaacaatata cgcatcccgt ttattctcaa
     7981 caacttaaga gtaatataaa gtcttataaa tgtttgacat tttgaaagct atagggcatg
     8041 ctttatttgt gaagtaatca aatatctttt tgcaaataaa atatagcaat ctatgacaaa
     8101 aacatcaaaa aaaattctta gcttaatcac gcttctgtgt tttgtattct cacagagtgt
     8161 tgatgcctat cctggtatgg gaattgagat gagaaccagc cgggagaccc cgtctttttt
     8221 tcaaattcag attccgtcgg atctcgccac ggtggacggt attttcgaag ctccggcgag
     8281 acccgacccg cgaatgatcc tccatgtcca gaacgcgcac gccaattacg acgcgcagca
     8341 aaagatcaaa cagctccttg agtatcttga aaagacctac gcgatcaaaa cgatcttcgt
     8401 tgaaggcgct tccgaagacc tgaatcccga ttacctgaaa tttttcccgg atgctgaacg
     8461 gaatcgaaaa ctcgcggatt acctggcgag gcagggcgaa ttgaccgggg cggaaatgtt
     8521 ccttctcgaa tccggggccg gggttcggag cgcggagcgc gataatgggg tgggcgcgcg
     8581 agaccccttg ccggtacggg ctcatggaat tgagcgggcg gaactttacc gcgagaacta
     8641 tgaggcgctc aagaaggttt ttggttccga agcggtcgtc agcaagtacc ttgagggatt
     8701 tgaagtgcgg cttgacaagt tggcgtccaa cgttttttta cccgatctcc ggaaaattct
     8761 tcgtgagtgg aaaaagtttg aaaatggcca ccgcgagttt attccgtacg tgaaggatct
     8821 tgtggcggat gccaatcgtt ttctcgggga agatctcgag agtttgttcg cgcaggttga
     8881 atggccgcaa atttcgaggc tcctcgttct ccagatgatg gagaaaaatc tggacgcgga
     8941 gaaggggatc tcggaaaagg aaagggttgt ccggttcctg aaagaaaaaa gagtttcaca
     9001 aaacctgatt accggcatcg aaacattcca ggaccagcgc gttaccatgc ctctgggaga
     9061 atccgcgggc ggaacggttc agccccggga tcttatggaa cagcttgtga tggaagcggg
     9121 ccccaaagga ttcctttttc acgattatcc ttacttcagc ctttatgccg gttatttgat
     9181 cctcaagagc gaacttgatc cgaagggtct ttttgacgag atcggcctgc ttttcagaaa
     9241 aatcctggac gaactcgcaa aaaccgacca gcagaaagca ctgcttgatc tttaccgcga
     9301 cgaagaactg gttcgcaaac tcctcaacct tgagcttacc cgcaagaact gggaagaagt
     9361 gctcgcccga aaagcccgtc ttgaaccgga agttctggtt gatcggttaa aagaacttgg
     9421 ggccacgaaa gagcaaaaac gtagtgcgtg gggcgaagac catggaacag ccggggaaaa
     9481 acgtgccccg gaacccgtgt cccggagtaa aaaattccgt gaaatcatcg acattcaaaa
     9541 cgccgcgtat gcgttttatg acgccgcgag gaggcgcgaa gaagtatttt atgaaaaaat
     9601 cgatgaaaca atgcgcgaaa ggcggatttc aaaagcggtc cttgttaccg gcggtttcca
     9661 cacggatggc atcaccgagc ttcttcgtga acatgagatc agctatggaa ttcttacgcc
     9721 gcgcctttcc gaaaaatccg acgagcatct ctaccgttcc ctcatgctcc agaaccagcc
     9781 cagcaccttc gagttatcgt acctggaagc gacttcacgc ctgatctctt ttgtgggaca
     9841 gacaaagcag atgaatcgcg aacaagttct cagaaatcta aagccggtct tgaaagcaat
     9901 cggttacagc ggaagcttta aaaatgtccg ccaggttatt gaggctttca atcattctag
     9961 cttttcggca gatgccggca ttcaaattga tccagaacca gtcaggaaaa agggagacaa
    10021 acctgtttac cgggttgttt taagcgggac attcgccacg aaagatcaaa ttcttggcgt
    10081 tattcagcga ttagcgacgg agttaccgaa gcctactgat aatcccgcgg cgcttgcttc
    10141 cattgcgaaa ttggcgcctg ccctagctca agcgggaacc agaaccggtg atgttgtgaa
    10201 aacaggagag gtcagaccgc cggatgacaa ggatgctgaa aagacagttc agagaattgt
    10261 tgttgcgcac gcgaaagcgg ccggagttcc taccgctgaa tatgccgctg ccttggtcag
    10321 ggagtttgcc agccactccg aaacgggaaa tgaaaatcac acatttgacg ttgcgcggcc
    10381 agccccccgc aaggaagcgc gggcggaaac gcgggtagct gagtttactt accgggaact
    10441 tttaaaagga tttatgacta tgttacggtc ccatcagcgg ccggttcacg ttcggggcat
    10501 ttggcctggc ggtggggtgg atccaaaccc aaatatcaga aactatatac aaactctgcg
    10561 gcttaacagg cgccagaaag tgattcccga tgtcattaga gtttaccttc cgataacaac
    10621 cgtggaggga caactggtta ccgggagtgg aaggtatgag gatagcatgg ttccgaaatc
    10681 ggaagagctt ttgcccgccg atgtttggca cgccttgcga caagcttcgg aagacggact
    10741 tttcgaactg gtggaattgg gcgatcgcgc ggcatatgac aatgcggcgc gagagttggg
    10801 tgttccggaa caaagttttt ttgaaactat ggtttctaat aatcgggtac tttttgtgtc
    10861 tgagtgggta gaagggacga ctttggacgc tgttcttgga aacacgcaaa atcctgatat
    10921 gcatagagaa gttggcagga atcttggccg tgttctgcgg gaattgcatc aaaatggtat
    10981 tttggtggat gataacaata ttgatcaata tatcgttact cctgagggtc aggttctgag
    11041 acttgatctc gagaaaatct atggggaggg tgcaccgcag gagggttttg aagcacaata
    11101 ttcttttttt gcggatgccc tgagacagga ttatccgttt ttttcagagt cttttgtgga
    11161 ggggtacgga gaaaacttgc gtgcaaatag aagcgaacag cgcagtcgca tcgaccaggt
    11221 tagggaaaaa ctgaatggca tgataggcat cggaggttgg gacagcatta ttaatgcggt
    11281 gagcaaggaa gaggttgaga agcatttgaa aagtttgaat gacggccaaa tgccctatat
    11341 tggcgagcat atcgatccac ggttattgga tatccatgaa aaaattgaaa ttttggcaat
    11401 gcgcgaggga ctttcctttg atgaggcgca taagcttgcc agggaatttg aacaactttt
    11461 tcgtgattgg ctgttggggc ctcgccgcgg agccacagcg gctttaatat ggccgtccgg
    11521 cgaagaaacg gttatttatc gcatgcgcga catgcctgga gatcatgaca tactggaact
    11581 ggtagtgaat cgaaaaggtg agctttatat tcgacgctcc gcttggcgca gggataagac
    11641 cacaggagaa tggggatggc aatacgcgga ttttatggat catttgatcc aattggttgg
    11701 gagcaggata aaggaggatc ctcgctttga aaattatcag aggatttatg aaaacctcat
    11761 ggttaccgtt gcattggaaa agaagtgggg gagtgcagtt gaggatctgg agttcatttc
    11821 gtcatacccg tatagtgaat taatagagat cggaaaaaaa tatggtattg actggaccga
    11881 agaagaatgg tttccgtttg cggcagatgt tctttttgcc gaggctggtg ttgaccaatt
    11941 catgcctcca gtgaaactgg cgatcaatcg ggcgcaaggg gaggcaatga gaaaaaaagg
    12001 atctgttcct tcgcctgctc aacgaccagc tcctattctt aaagccatga catttgatgg
    12061 gagaacagtt gaagttccct cctcccgctc cgaatcgcgg gctgatacga gagccgacgc
    12121 cgtccgatcc gaaagccggg catcccaacc cgacgaatcg atatatgacg gcatgtcgga
    12181 tttacagaaa gaaattttta atatatggaa aaagtttgct gatcctgctt taaacaggat
    12241 ccaagatgct cagccggtta ttgaagtgat gcgagcgatt gttgacgcaa tacgagagat
    12301 cggggaggaa gaattttatc gggaactgga aaaaacaaaa aagcatctgg caaaagctct
    12361 ggaatcggta gacgactatg ttgctctttg gggagggccg catacctccc ggcggtgggt
    12421 agggctccag attctggaag gcataaagcc tccgaaacta gtaacttatt ttgaacccga
    12481 taaatattac ttcggaccgc gcatccggga aatagccttc gcggctgata caattgtgat
    12541 cgcggatgat gcttcttact cggggaagca gatcagggaa actatggagt ttatattgaa
    12601 ggttaatccc aacgcgaaat tcattatagc tgtgcctttt atgtcggata ctgccgtccg
    12661 taagattaaa tccaagggta cattgtccca aaaaccgtcc gtcaaaatcc ttcctcacaa
    12721 caaaatactg acagtaaaag agattcttaa tcaaaaaaac attcttacac aaaaagaatt
    12781 ttctactccc gagggtgttt ttcggaacaa tccgggtgtt tttggaatca accctgaccg
    12841 gcttctcaca cagtccgtga cattattgtg gtacaaggtt tcgaatgccg tgtcattcta
    12901 tgcgacgatc gttgcgattg ttcaatctgt taaagatgtg acacctcctt atcaagaaga
    12961 ggatagcgat tactacagga aagagaccga agaataccgg ggatttgttt tcggttcccg
    13021 accggagtcc cgtcagccaa cagggatgtt ggcgggaaaa ccaaaagctg aatctcggca
    13081 ggagcaagct cgttcggaag tccgggcatc ccaacccgac gaatcgatat acgacggcat
    13141 gtcggaatct gctgaagaaa ttttagataa gtggatgggg ataaagagtg ttgcgtctgc
    13201 tttaagcagg atccaaggtg ctcagcgggt tcgggttatt gaagtgatgc gaaaaattgt
    13261 tggcgcgagg cgagtgatag ggaaaaaaga atttgagcag gaattggaaa aaacaaaaaa
    13321 gcatctggcc aaagctctgg agccggaaga atcgaaagtc cgccatgttg ttctctttag
    13381 agggccgcat acctccccgc ggtgggtatg gtcccagatc cgggaaggaa taaggtcctt
    13441 gaaaggagaa acttattttg aaccctatga agattatttc gaaaagggcg gccttatcag
    13501 aaaaatagct ttttcagctg acacattcgt gatcgcggat gatgcttctt actcgggaga
    13561 gcagatcagg gaaactatgg agtttatatt gaaggttaag cccaaagcga aattcattat
    13621 agctgtgcct ttcatgacgg attatgccgt ccgtaagatt aaatccaagg gtacattgtc
    13681 ccaaaaaccg tccgtcgaaa tccttcctca caaaaaaatt ctgatattgc aagatattct
    13741 gactgataat gagcagaaaa tatttagcca atattcgaat gcggatattt ttggaaaaga
    13801 atttgttggt gggaaagaac gtaatcttct cactacgacc gtgacactat tatggtataa
    13861 aattccggat ggctggtcat tctatgggct actttctcaa gctattcaac ttgttcgact
    13921 tgcccagcat gctcaacctg tgattccccc ctacaggcgg caaaatagta aatacttttc
    13981 ggacgaggag aaagaataca aggcatttct tcagaagtct cgctccgaag cgcgggttgg
    14041 agaagaaacg acagcgccgg accgcgctgg gctgggcgtt ggtctggaat attgccgtgt
    14101 ccatgaaaaa gcgttgcgag cggaggggct tgatctacgg gaaagcccat tgtctaaagg
    14161 atcggtggaa ggaagggatt tttatgattt tgccgcgcgt ttcaatacca tggaggaggc
    14221 ggcaaaacga cttgcggata tttttattgg tatcaaccgt tccgaggagt ttccggattt
    14281 acgccttgaa gatccgggat actggaaacg ctatttggag tggattgcgg gtgcagaaga
    14341 gaaatccagg cagcaacttt acaagattat ggccgagagt gaatatcgtt ctcttcggag
    14401 ccatcaggtt ttctgggatt cttatcgtga tcatcaggga gcgttagcgc aagaggggtt
    14461 acaaataaag gaaacggggg aagtagtttt aactttcagg ggcgccgaaa aaatgacggc
    14521 aaccgaaaag gcctactgga taagccgact ttttaataaa cacaatattc taggaggcga
    14581 gtttgcggat cgttcttcgc aacggggctt cggaggatcg gttgtctcga gggatgtgct
    14641 cgcgtgggtg cgttatttta accgccaaca cgggtatgcc ggagttccgc acggcaccag
    14701 gagattttgg gagatctggc gtcctcgcga tattaaggaa agggaatact ttttgatcga
    14761 gagtttgagg agcctaacac cctcttaccc ggctcccttt tcaaagaaag ccgatgaggc
    14821 tatacgtgag gccgaagccg ggaaaataaa agaacctatc aaaaggaaaa gccgagttgg
    14881 atcaccgatt attttatctg gccccccgga agttgccacc gtcataacaa cgtctcccgc
    14941 gtctgatttt ctggctccgg gtacggtgct ggatccccga ggaaaaccta ttgtgccgcg
    15001 ggcgccacct tctggtgaaa ggaaaatcag cgttggtata tggcttgcca tcgcttcagg
    15061 atggatcgtg gctatcacct tagcggccat tttattttat tcgcatttat tttattcaca
    15121 gccgccgcaa agtgtttcaa tcgctgattc cgggtatcat gtcgagtttc ccgcagaagg
    15181 cgtcttggag agccctcacg cttttgaccg tgaacagttg cttgaaagcg gaaggggtga
    15241 gggagaattg cctatacata tcagcagttg ggtggaggaa ctttcagaaa atccttctcc
    15301 ggtttccccg gtaattgggg gcccgtcttc cttcctggtt tatttgacct atccgctaaa
    15361 aacattgact aaaccatcgg ataggccctt tacccgtcag ggggttgata cccacgcgat
    15421 gcggattttt accggagaac tcggagaacc gtccattctc aacgtgaaag gagggacatt
    15481 gccggccgga tttttgcctc tatacatttc gcccgggttt acggcggacg gatcgcggca
    15541 atcaagccaa cccatcgtaa tggaatcggg gcaacctcgt gacgacggag aggttcgttt
    15601 cgaagaagtg gaaatcattc cggcttcatt taatggcaaa aggattcccg tcccggatcg
    15661 ctgggtggtc acagatttta aagtaaacgg gcagacggcg cccgcgggtc aatctttaga
    15721 tttcggtacc tggggtatta cggttcagcc taacggcatt gtttcgactt ccggttcttc
    15781 tcctcatgcc gggacgaaag tggaggcaac agtccgggaa ggtgaaggtc tggccaagac
    15841 gttgaatcct ccgggttcga atgaggtcga tgatgcccgg gttgcacgcg aaataatcga
    15901 tatccccaag gaaatccggg gtgagcttga taatatccgg gctcggtttg agaacggcga
    15961 gttaacccgg gaaggcgttg ccggggctgt tcatgaactt atgaaacaat acgtacgcta
    16021 ttatttggat cataaacaag cgtctctgga tagagatgag acctggagca attattttag
    16081 aagggcaatc acggacaacg gttacattgt tgctaactgt gatgaccttg ccacacttga
    16141 aaggatctgg ttgctctaca cagggttgcc tccggaagac gtgaatttcg tgtcaggaat
    16201 tcttactgac cccaaaagcc cgcatagcag gtttatcggc ccgacgcaac cccactcgga
    16261 taaaactctg cacgcgacct tgaggattgc cactccgggt tctggttcgt ccgaaatcat
    16321 ccatgaagca acgcggggtg ttggagttgt cgaagaaaac caatacgaaa tcacgaaatc
    16381 cagcgatttg cgggaccatg tcagggacgc tcaggaagca atcagggcat acaagagcgg
    16441 cgaccggaat ccgcaaagtt gggatatcgg gctcacggac cttgcggatg cgttgctagg
    16501 agaagacttg agtccgggac aaagaacaac agtcgcaaag atcatcgcgg agaattatct
    16561 ttacgcgttg ggtaaaaccg gacacaggcc tgcaaatgag ctctccggac ggatcttgtt
    16621 tgagacggca aacatcctcc tccgggatgg agaattatct gatgaattga aggcgagtct
    16681 tgacgaaatt cgtgaaaaaa tcgttggctg gattaaagaa aaagtaagcg aagaaacgcc
    16741 tctttccagt gtcttgaagg atcccgggac ggccgacgcg tacaatttgt ttttcaacga
    16801 atgccaggct gatttgtcgg cagatcttga cagcttatgg tcgcttgcca tgagcagggt
    16861 tgatccctgg accgaactct ccgtggatgc gaaaactgcc atgagcgccc ttcccgaaga
    16921 tgcggagggt ggcgattcct tccatgttcg catgctgaaa agtttcgcga caggggagcc
    16981 tgtgaatccc tccgacacgc ttgaggcgct tggcagggag gacgtaagca attcggtcaa
    17041 agccgaattg ggagagaaac ttcgtgcaac gacggaaacg ggagaagata tggttgcctt
    17101 gctagaggcc tgggcccggc tcgatccgaa cagtgaccag ccgggtgaga aacaactcca
    17161 tattgaaaaa ggtttgatta attcattcaa tcccgtgatt acccttgaaa cgccctgtgt
    17221 gactattacg gggcttgtcg atatggccaa aagcatgagt ccttctaatc gttacatgtt
    17281 tctgggcaag gatccgcgtc ttgcggccct ggccgcttac atgacagggg aaaatatccc
    17341 gcaaaacggc caggagcttg gtggttatct ggctacgcag ttggcaaaaa cgttatcatc
    17401 ggaagcctac gcatatctgg cggatctatc aaaaccgtta gaaggactgc cggtgcttcg
    17461 atttttcgga atgctgagcg gagaaggtaa cgagattcgg ggaactattc gcggcaatgc
    17521 gcctttggat aacttgctca agaaattatt taacgtggag aaagtcggaa tttccgaact
    17581 cagattcttg agagaaacaa tcatccggga aaaggagccc gacacggaaa cgcaaccttc
    17641 cgcgcgacag gaagtgcgca cgtcccgctc cgaagcgcgc gcggaaattc gggcaaaaca
    17701 gctggagctt tttgaggatg tgactttggg cgcgcggact ttgacgatcg gcgtggccca
    17761 actcgcaaaa tatctggagg cggggatcct cgcgcggcag ggcgatggaa gggtcgattt
    17821 agggcacagg gccgactcaa aaatgcgggg ggcggttggt gctgtcaatt cgggatttgc
    17881 tgtgcgcagg caagaatttt cagtaggagg caagacattt tcagcagggg agcttttaat
    17941 tcaaatcctg gacggcaaca ttgagttcgt ggatgaccgt gatcctggtg atatttacga
    18001 tcgcgctccc ggcggccatt tcgcttttcg cggtgaaaag cgttctgaag ttcgcggtag
    18061 ccaacttgcg gttgtgcagg gggagacttt gatcatgcct ccggatgatc gaaatctggt
    18121 gagattgggg ccatgggtcg tgtcggggca ttatcttcca gcacactctg gctggaagct
    18181 tcacattcaa atgg
//
LOCUS       DAOSIO010000010        17688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_3410_length_17688_cov_4.292293, whole genome shotgun sequence.
ACCESSION   DAOSIO010000010 DAOSIO010000000
VERSION     DAOSIO010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 17688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17688
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3410_length_17688_cov_4.292293"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1928
                     /gene="dnaE"
                     /locus_tag="PK590_00860"
     CDS             <1..1928
                     /gene="dnaE"
                     /locus_tag="PK590_00860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOG23178.1"
                     /translation="RHASIHAAGVVISDVPLTERVPLFKTEDQVCTQFTMKDLEKIGL
                     LKMDFLGLKTLTVIDKAVKIVKKMHGVNLDIHHLPEDDPTTYEMLARGDAFGIFQLES
                     SGMRDLLKKMKPSKFEHLVALLALYRPGPLGSGMVDDFVKRMHNPKLIQYDHPGLEPS
                     LKETYGVILYQEQVMRIVSDLAGFTLAQADSLRRAIGKKIPEVMAKEKKAFIDGASKH
                     GVKERIAEKIWDQIDYFSGYGFNKSHSTAYAAVSYQTAYLKAHYPVEFMTALLTSEMN
                     NTDKVVQYIEACKKIGVDVLPPAVNESFKEFTCIGKNIRFGLAAIKNVGTTAIESIIT
                     SREKDGAFKNFFEFVQRVDLRVCNRKVLESLIKCGAFDEWKLHRSQLMAMLDTALEMG
                     SNLQKDRNRGQMSFFENTVGEGTFHDEQATIPAIPEWPENQKLAFERELIGFYVSSHP
                     LSKYTKVLKNYATATSDTLPEFNHQAEATMGGIVDQLKEILTKKGDKMAFLTLQDLVG
                     SCEVVVFPSVYRETSNLIQKDQLIFVRGKVDARDDPPKILADEIVPIDEVPKRFTRLV
                     AINLKTAGLVPDTLKEVRRILLRHKGTVPVYISLMDPKGRRTVFDSGENLKVETSDAL
                     FDELESLLGENSVKIRS"
     gene            2139..2411
                     /locus_tag="PK590_00865"
     CDS             2139..2411
                     /locus_tag="PK590_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOG23179.1"
                     /translation="MTKKDIVMKVSNETNLTQIDVKKIVQKTLDVISESLQAGETVEL
                     RNFGVFKVKTRRGRLGRNPRTGQEVTVPEKKVVVFKPGLILKVKVK"
     gene            2483..2761
                     /locus_tag="PK590_00870"
     CDS             2483..2761
                     /locus_tag="PK590_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOG23180.1"
                     /translation="MADKGKVKWFSNVKGYGFLRKDGEERDIFVHYSAIQGDGYKRLN
                     PDEEVEFEVVEGEKGPQAQNVVRTKKPKEEPADGAGESKPEEKKKDGK"
     gene            2870..3682
                     /locus_tag="PK590_00875"
     CDS             2870..3682
                     /locus_tag="PK590_00875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOG23181.1"
                     /translation="MIARDHDSRPHERRLRRFYIPQPLSQAGDFIRLDASQTHHLRHI
                     IRLSLGDHCLVTDGQGWEGEAEVREFTRDGGTKLAICRITRTTEPRTNKTVFRVMPAL
                     LQKGKTDFLIEKAQELGLDEFWPILSDRCEIKIPEEKLVKTVDRWRRIAIEASKQSGA
                     LMAVKISEPVAFRKAIETLAEDDGLLIFHPSPEAVPFARWINERSQVADPLKSLNILI
                     GPEGGFTDDEIEWTLWRGKKKNVWIVSLGDTVLRADTAFISILGTLRFLEIV"
     gene            3750..5102
                     /gene="mtaB"
                     /locus_tag="PK590_00880"
     CDS             3750..5102
                     /gene="mtaB"
                     /locus_tag="PK590_00880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOG23182.1"
                     /translation="MMKFTIKTFGCKVNQYESQAIRESLQAGGAREIEKPGEPADLVI
                     VNTCTVTRDADRSACYWIRRLKRENPDSRLAVTGCLAERDRAILEGMKEVDFIFKNQE
                     KQRIADFLIAGTPKTKSVGMVSDRAEHKGFLPLSVSMSDGKTRATVKIQDGCNNACSY
                     CKVVLARGPSRSRSLGDILDEVRRLADNGYKEIVLAGVQLGSYRDGIGDHSNSLVRVI
                     EKCSKIQGIVRIRLSSIELTDIRDPLIRSFRDIPKLCPHLHIPIQSGSNSVLKNMNRR
                     YSAEDCVKLIEKMRSKIPDFCLTTDVIVGFPGESEDDFGESLKVLETLRPLKSHVFPY
                     SPREGTRAALLRGLPGRVVRDRANRMIRRMDCVSREVRKAFIGKTVEVLVEHRNPRSR
                     YREGRTANGLRVFFDFPKEGQGCIIPVVLRELFEDGIAGTWQNSGEGDRDGFFMESDR
                     "
     gene            5074..5661
                     /locus_tag="PK590_00885"
     CDS             5074..5661
                     /locus_tag="PK590_00885"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOG23183.1"
                     /translation="MDFLWNLIDSIQKSAMYLVIKDLAPIDWVAIFFVLFGMLQGSRK
                     GFIDMLAKSLNVTIVIILTLTFYPLISAYLVKTIQALPKAVADPLVFIPTGVVAWLVT
                     GWCFNAIGRIFKVEVSSFLKFFGGVFFGGIYSVLLLSLFVQMVLLIPGEMTQKPFTQR
                     GSYSGQALAKLAPGVQRFVAAPFVGTRESRSRPAV"
     gene            5666..7459
                     /gene="dnaG"
                     /locus_tag="PK590_00890"
     CDS             5666..7459
                     /gene="dnaG"
                     /locus_tag="PK590_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOG23184.1"
                     /translation="MALDLPRNREQEDIVERVAAANDIVEVISQFVPLKRAGRNFKAC
                     CPFHQEKTPSFMVQPEKQLFHCFGCQAGGDVFSFLMKYENLSFPEALKMLADRARITL
                     PEKRYAKGDSSHKDQIYEIYQCAVELYQRLLAGESGKKARDYLAKRGMTDETLREFKV
                     GWAPDEWRTLYDHLLKKGYPEALLKQSSLIQQSAKGNYYDTFRGRILFPIMNLQNKPV
                     AFGGRLLGDGEGPKYLNSSENPVFSKRRELFGLNVAKRYIDANWPNLILVEGYMDFLA
                     LYQAGFKNTVATLGTALGEDHARLMKRFAEEVIVVYDGDDAGESAALRGLEILLEGGG
                     QVKLASLPKGFDPDDFVRKRGADAFRELLKNAQDFFDYKLKTLLERYPKNDALGLAKI
                     SREMLETFRKVKNPVMLAEYFKRLSREIGIDERSLRAEFENISRRGSDLARRRGEKTV
                     EKKTAPATPQEEVLLLALMMENSNFLDEAERQLNPETMRSPVSRELFKFLVDSGGVAK
                     KKPLSTVLNKIQNTAYREQLVGALAALEDTPDRKQIFQDCLRTIRRNGALDRQEKLRG
                     LIHEAERNGDSAKLLAYTREFQEQLKETREI"
     gene            7651..8292
                     /locus_tag="PK590_00895"
     CDS             7651..8292
                     /locus_tag="PK590_00895"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG23185.1"
                     /translation="MKSLIAPIRCWQRNLLDTLVRKYYHQALEARAEGMVRLFERDIP
                     ERCRILDLGGGWGFYNEPFKRRGHESLILDVVKPGYQKAPVVIYDGGRIPFGDRAFDV
                     TLMATMLHHVPDPESMMAEVRRVTRTKVVVIEDLYHHAIGRLWTIARDRILNMEFLGH
                     PHQFRKHEEWIQFFREKDFRLEKFRKFYTWISGFRILNGLYVWECGENVGAIG"
     gene            8273..8716
                     /locus_tag="PK590_00900"
     CDS             8273..8716
                     /locus_tag="PK590_00900"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23186.1"
                     /translation="MSVLSVEQVLWMNEGEGITARGKIPHDSEFFKDHFPEFPVLPGV
                     LAIEMLKQTAEKYLESFSEDGAGGSRFFLKQIRATRFRKYLRPGDEWESRLTLTQSLE
                     GETVWDAKLYYHGEVAVSAKLTLARIKHPPVGASDIPLTEGKGEL"
     gene            8713..9120
                     /locus_tag="PK590_00905"
     CDS             8713..9120
                     /locus_tag="PK590_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOG23187.1"
                     /translation="MNQEIFKKVQETLAGALGVETSEITPESSLKRDLGAESIDFIDI
                     IFRLEKSFDLKIPANDLFPSYIFTDERFVKEGAVTPEGIQELRTKLPYLELGDFEKDP
                     QVAELADYFTVKMILRYLEERLGKSSGCSGACQ"
     gene            9162..9578
                     /locus_tag="PK590_00910"
     CDS             9162..9578
                     /locus_tag="PK590_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOG23188.1"
                     /translation="MSRSPQEKNTPGWILRGRPKTLHGIGIDIVSRSRMKKFLSRHRE
                     MILRRFLNSREREQFSEIRLTRLAELFTAKEAFFKACNEPWMGLEGFQGMRITKRIGD
                     YFEMEWHVAGTRRCYRGDGSFFRDGDLVGAQAMIWS"
     gene            9581..10015
                     /locus_tag="PK590_00915"
     CDS             9581..10015
                     /locus_tag="PK590_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23189.1"
                     /translation="MRWLLLDQVVAVHRGKKAVTLSRVPQAPYSREVLFIEMMAQTGG
                     LLVGAESDYNNDVIFAKIETATFTEPPAPGTALTVEASADGLAPEGGWIEGQITSSGG
                     VVAKAKLLLMSVGRLIPESQRSIAFHDNFMNHFKVREKVTVS"
     gene            10012..11301
                     /locus_tag="PK590_00920"
     CDS             10012..11301
                     /locus_tag="PK590_00920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOG23190.1"
                     /translation="MTRRRVVVTGFGVMSACGKGWSPYWNAAVHGQSAIREVEHLKLN
                     GSSLKYAGFIADFDPKQFVTNRKSLKVMSREIQLAVAAGKLALEDAKLLDMAYDQSRI
                     GVVLGTGVINNDLDEIGAGFKSAIDGSGRFSMAQFGQDGVRSLYPLWFLKYLPNMPAC
                     HVSIAYHLRGPSNTITTSSAASAQAIGEAYRVIERGDADIMLAGGTDSKLNAMGISRF
                     NLLGLLSTRTGAPETVYSPFDEKHDGIVLGEGAGIMVLESLENAKRRGAMIYAEITGY
                     GSSSDFNFDPRDEDDFTGKRVAMKRALEDSGIEHHDVDVIVANGSGVPQADIQEALAI
                     HSFYMGSFGKLNVTALKPLTGNLVYASGGVEAVIAVLIANKKTIPPVANLNKPYPECP
                     LPLVKGKARSAEVQFVMLNNFGLGGQNASLVFKHVKT"
     gene            11288..12580
                     /locus_tag="PK590_00925"
     CDS             11288..12580
                     /locus_tag="PK590_00925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOG23191.1"
                     /translation="MSKRRVVVTGVGAVTPLGNNVRDTWLRVTQGKSGVGPITLFDAS
                     TFATRIAGEVKGFNFERWIERNPPLASAARGTFFALHAAAEAYKDAMLKPFTVDSRRF
                     GIYFGGGDSGIDFDPFVKTLMASFAASGDLVDKGEYVHQSLRHMNALRELESQPFMTV
                     THLTRLFDIRGPVSNCLTACAASSQAIGEAYEWIKRGDADVMMTGGSHSMIYPLGVVG
                     FSLLTALSTRNDEPQRASRPFEKKRDGFVIAEGAGVLILEELSHAQKRKAHIYGEVVG
                     YGSTADSYRLTDMDPEARGASEAMRIAMTKAGIEPADVDYINAHGTSTGVNDSLETVV
                     IKKVMGEYAPKVPVSSTKSMLGHMIAAAGGVEAIFCLLAMRDSIIPATINYEEPDPEL
                     DLDYVPNTARRASVNVALSNSFGFGGQNICLALRKYNG"
     gene            12573..13664
                     /locus_tag="PK590_00930"
     CDS             12573..13664
                     /locus_tag="PK590_00930"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG23192.1"
                     /translation="MANNLIFMTGATGVLGQELVKELLLTTDATIAILSRAKNRVPHD
                     ERVQKILKATGMDAHFGSRVRVLEGDVTRSKFGLKKSDLDLLERDCKVFYHIAALTAL
                     NGTEKECQEINVGGTKNALELAWDLRKKGSLERFVYFSTAFVAGSVWHYRSPEDSLPE
                     KPAFANYYESSKHQAEGMVREAMGQGMPVTIIRPSIVVGHSETGEVSDFNVIYPFIKL
                     FAHGILTKLPTRPENTFNIVPIDFVIKATAAIMRNPRSINKCFHLVAPNPPAIGMLLS
                     LRDAFPKSAVIEMVEPSSFQKAQLDPQQQMVFAMLEPYLGYLNGDLTFDTKNTEEILR
                     GTGIKMPVTDLAFLKTLITYAVKSGYLVI"
     gene            13866..15272
                     /locus_tag="PK590_00935"
     CDS             13866..15272
                     /locus_tag="PK590_00935"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG23193.1"
                     /translation="MKVLLIEPPPASKFGNLRTLGSIGTLKADMAWPPLDLMIIAGLL
                     KQHSIPVALYDANSLKATFEDVQKKIESQKPDLVIFTTSTPSLYHDLQTARVAKRVSR
                     NILTAVTSTHVNALPKETLALSDDLDFCIPNDSEGTVLDLIQKDGRPEDVLGLTYRKD
                     GKIIQNAPRPVCTNLDELGIPAHDLIDLDIYRDPFTKRMPMAVTYTSRGCINYPPCVM
                     CSACFYNRVRYRSTELVMRELRWLKSLGVQELRFPFEAGFNDNRRAHELFDAMIQEKL
                     GMTFTCNGRADCLSEEKVRQMKAAGCRAINIGCESTHPETVEFIKKRVSAEQVVRAVA
                     MAKKAGLETLVYFLFGFPYETKESMKKTLEFAKRLDADLVTFGIAIPHPGTEFYRYLK
                     DHGLLSSDQWDLYDPMFPPPYAYPELSSREIFDFARYAYRSYYLRPRFILKRFLKFNL
                     VRETKNFLGFMNRYVTGS"
     gene            15269..16690
                     /locus_tag="PK590_00940"
     CDS             15269..16690
                     /locus_tag="PK590_00940"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG23194.1"
                     /translation="MTRLLLLHPSGKLLGRKDNLYTNESLVPSLGLASVAAYCRACGI
                     ETRIYDLRLENNGIEGVFERIDDLNPALVGITAFTTEITSAGVLASRIKQKYPDLQIS
                     VGGPHGTAMPQETLLEFEAFDSVVCGEGEKTTAELVVATQVGGCPDLSKIPGLVYRKE
                     GKIFSNPTREPVADLDTLPFPAWDLFELERYNGIFPLSASRGCPYRCYFCTPNYLGKK
                     VRVKSHQKIMEEITWLVERFGAKRIQFTDAMMGLLKDESIQMCDELIRSGLGREIQWD
                     CETRADAVSLGLFQKMKEAGCEWVALGVESGNERILGEVIKKGETKDQIRVAVQLAKR
                     AGLKVRTFFILGHYTETPETIRETIRFALELNPDAIAFGLIVPNPGSEFRKIAETKGS
                     GFRILHNRWEDYQQFNYNCLESETFSLAELKRWQARAYFTFYAHHPIKGFFLFFAQSA
                     YNYRLSGLLKLPLMLLRNLLGKS"
     gene            16848..>17688
                     /locus_tag="PK590_00945"
     CDS             16848..>17688
                     /locus_tag="PK590_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG23195.1"
                     /translation="MTESLPQKTRVAVVLPCYRVLPHIQKTLAGIGPEVWRIFVVDDA
                     CPDQSGDFVKRHCRDPRIEVLYHDKNQGVGGAVLTGYQAALDAGADIIVKLDGDGQMD
                     PGLVTDVIAPIVAGEADYTKGNRFFDPEGVRTMPRLRLFGNAVLSLMTKLSSGYWNLF
                     DPTNGYTAIHARVARRLPFHKISKGYFFETDILFRLNTLRAVVMDVPMNARYGTEKSN
                     LKVSRILGEFLFKHLRNFLKRIFYNYYLRDMSLASLELPLGVFFFFSGGFYGACHWAR
                     SSQA"
BASE COUNT         4228 a         4055 c         4996 g         4409 t
ORIGIN      
        1 cccgtcacgc ctctatccac gcggccggtg tggtgatctc ggatgtcccg ttgacggagc
       61 gggtcccgct cttcaagact gaagatcagg tttgcactca gttcacgatg aaagaccttg
      121 aaaagattgg gcttctcaaa atggattttc ttggtctcaa gacgctgact gtcattgata
      181 aagcggtcaa aattgtgaaa aagatgcatg gggtgaatct cgatattcac catcttcccg
      241 aagatgatcc cacgacttat gagatgctcg cgagggggga tgccttcgga atttttcagc
      301 tggaaagttc cgggatgcgg gatcttctta aaaaaatgaa accgtcgaaa tttgagcatc
      361 tggtcgcgtt gctcgcgctg taccgtccag gccctcttgg aagcggcatg gtggacgatt
      421 ttgtgaagcg aatgcataac ccgaaattga ttcagtacga tcatccgggc cttgagccga
      481 gcctgaagga aacttacgga gtgatcctct atcaggagca ggtgatgcga attgtaagcg
      541 acctcgccgg ttttacgctc gcccaagcgg actcgctccg tcgcgcgatc gggaaaaaga
      601 ttccggaggt catggcgaag gaaaagaagg ccttcattga tggggcgtcc aagcacggcg
      661 ttaaggaaag gattgccgaa aaaatatggg atcagattga ttacttttcc ggatatggtt
      721 ttaataaatc gcactcgacg gcgtatgccg cggtatcgta tcagaccgct tatctgaagg
      781 cgcattaccc cgttgaattt atgaccgccc ttctgacctc cgaaatgaac aatacggaca
      841 aggtggtgca gtacatcgag gcctgcaaaa agatcggggt tgatgtgctt ccgcccgccg
      901 tcaacgaaag cttcaaggaa tttacctgta ttggaaaaaa catcaggttt ggcttggcgg
      961 cgatcaaaaa cgttgggacg accgcgattg aatcgattat cacgagccgc gaaaaggatg
     1021 gggcgttcaa gaacttcttc gaatttgttc aacgggtcga tctgcgggtc tgcaaccgta
     1081 aggtgctcga aagtcttatc aagtgcggcg cgtttgatga atggaagctc caccgctccc
     1141 agctgatggc gatgcttgat accgcgctcg agatgggatc taatttgcaa aaagaccgga
     1201 atcgcggaca gatgtctttt tttgaaaata ccgttgggga aggaacattc cacgatgaac
     1261 aggctactat tcccgctatt cccgagtggc ccgaaaatca gaaacttgcc tttgaacggg
     1321 agcttatcgg attttatgtt tcatctcatc ccttatcgaa atatacgaaa gttctcaaga
     1381 attacgccac cgcgacctca gatactctgc cggagtttaa tcatcaggcg gaagcgacga
     1441 tgggcgggat cgttgatcaa ctgaaagaaa ttctgacgaa aaaaggggac aaaatggcct
     1501 ttttgacttt gcaggatctg gtcgggtcct gtgaggtagt ggtattcccg agcgtttaca
     1561 gggagacatc gaatctgatt cagaaagatc agcttatttt cgtgcgcggc aaggtggatg
     1621 cccgcgacga cccccctaaa attctggcgg atgagattgt tccgatcgac gaagtgccga
     1681 agcggtttac ccgacttgtg gcgatcaatc tcaaaacggc agggcttgtc cccgacacgc
     1741 ttaaagaggt ccgacgtatc ctccttcgtc acaaaggaac ggttcctgtc tacatcagcc
     1801 tgatggatcc caaaggccgg cgcacggttt ttgattcagg cgaaaatctc aaagtggaaa
     1861 cctcggacgc cctttttgat gagctggaat ctcttctcgg tgaaaattcc gtcaaaatcc
     1921 gttcgtgata tgtgatcagg agttttgttc aagcggaatg gatgtacctg ttttttgggg
     1981 ggaaatgttg ctttatttcg ttaactccct gtgtttattg aatttatgat gaaatcgctt
     2041 gacggcattc gccggatttg ctatctttct ctatcttcaa acaggacaag cagttgggat
     2101 aattccaacc gtgttacttc atgaaggagg tgagaatttt gacgaaaaaa gatattgtca
     2161 tgaaagtttc taacgaaacc aatctcaccc aaattgatgt caaaaagatt gttcaaaaaa
     2221 ctctcgatgt tatttctgaa agtctccagg cgggggaaac ggtcgaactt cggaatttcg
     2281 gtgtctttaa ggtgaaaacg cgcaggggac gtcttgggcg taaccctcgt accggccagg
     2341 aggtcacggt tccggaaaaa aaagttgtcg ttttcaaacc gggtcttatt ttaaaagtga
     2401 aagtgaagtg agcggctgtg ttttgttcgt gatcccacac gggatgattt ttgaagtttg
     2461 aatctaaaag gaggatgcga ggatggctga taaaggtaag gtgaagtggt tttcgaacgt
     2521 gaaaggctat ggtttcctgc gcaaggatgg cgaagagcgg gatatttttg ttcattattc
     2581 cgcgattcag ggagacggtt ataaacggct caacccggat gaggaagtgg aatttgaggt
     2641 tgtcgaaggc gagaagggac cgcaagccca aaacgttgtc cgtaccaaaa agcccaagga
     2701 agaaccggct gatggcgcgg gagaatccaa gccggaagaa aagaagaaag acggcaaata
     2761 aaaacagcgt tgtattcacc tgaaatcatt ccataaagct aaggaagctc ggaccatggt
     2821 ctgagcttcc ttgtctgttt ataacccgat tattttgagg ttgaaagcca tgatagcccg
     2881 cgatcacgat tcccgtcctc atgaaaggcg gcttcggcgt ttttacattc ctcagccgtt
     2941 aagtcaagct ggagacttta tccggcttga cgcctcacaa actcatcatc ttcgccatat
     3001 cattcgattg tcactcgggg atcattgtct tgtaacggat ggacagggct gggaggggga
     3061 agctgaggtc cgggaattta cccgggatgg cggtacgaaa cttgctatct gccggattac
     3121 tagaaccacg gagccgcgga cgaataaaac tgtcttcagg gtcatgcccg cgttgcttca
     3181 aaagggcaag accgattttt taatcgagaa agcccaggaa ctcgggcttg atgagttttg
     3241 gccgattctg agtgatcgtt gcgaaataaa aataccggaa gagaaactcg tcaaaacggt
     3301 agatcggtgg cggaggatag cgattgaggc tagcaaacaa tcaggcgccc tcatggccgt
     3361 caaaatatcc gaacctgtcg cgttcagaaa ggcgatcgaa accctggctg aagatgacgg
     3421 ccttctgatt tttcatccgt cacccgaggc tgtacccttc gcgcgatgga tcaacgagcg
     3481 ctcccaagta gcggatcccc tgaaatcact caacattctg atcggtcctg aaggtggttt
     3541 tacggacgat gaaattgaat ggaccctgtg gcgggggaaa aaaaagaacg tgtggatcgt
     3601 gagtctgggt gatactgtgc ttcgggcgga caccgctttt atcagtatcc tcggtacgct
     3661 tcgttttctc gaaattgttt gaaggattta tgctctcccc aaagttcagg attttaaacc
     3721 gtggtgtccc cagggttcct tgaatttcaa tgatgaaatt tacgatcaaa acatttggct
     3781 gtaaggtcaa ccagtatgaa tctcaggcca ttcgcgaatc cctgcaggct gggggggctc
     3841 gtgagattga aaagcccgga gagccggcgg acctggttat tgtcaatacc tgtacggtga
     3901 cgcgtgatgc ggatcgttcg gcgtgctact ggatccggag attgaagcga gaaaatccgg
     3961 atagccgtct cgcggtgacg gggtgcctgg ccgagcggga tcgagcgatc cttgaaggaa
     4021 tgaaagaagt tgattttatc ttcaagaatc aggaaaaaca aaggatcgcg gattttctta
     4081 tagcgggaac tccaaagacg aaatccgtcg ggatggtttc tgatagggcg gagcataagg
     4141 ggtttttgcc actttcggtg agcatgagtg atggcaagac ccgcgcgact gtcaagattc
     4201 aggatggatg taacaacgcg tgtagctact gtaaagtggt tttggctcgt gggccttcac
     4261 ggagccgttc tttgggggat atccttgatg aagtaaggcg gctcgcggac aatggctata
     4321 aagaaatagt tctggcgggt gttcagctgg gatcctacag ggatggcatc ggggatcatt
     4381 ctaattcgct tgtgcgggtt atcgagaagt gctccaaaat tcagggaatt gtacgaattc
     4441 gcctgagctc cattgaactg acggatatcc gtgatccctt gatccgttca ttccgcgaca
     4501 ttcccaaact ttgccctcat ttgcatattc cgattcagag cggttcaaat tcagttctga
     4561 aaaatatgaa tcgccggtat tccgcggagg attgtgtgaa gctcatcgaa aagatgagat
     4621 caaaaattcc ggatttttgt ttgacgaccg atgtgattgt cggtttcccg ggtgaaagcg
     4681 aggatgattt cggcgagagc ctcaaggtcc ttgagacctt gcgtccgctc aaaagtcatg
     4741 tttttcctta cagcccccgc gagggaacgc gagcggctct tctccggggg ttgcctggac
     4801 gggtggtgcg cgatcgcgcg aatcggatga tcagacggat ggattgcgtt agccgggagg
     4861 tgaggaaggc atttatcgga aagacggtcg aggtgcttgt ggaacacagg aacccccgga
     4921 gccgttaccg ggaaggaagg acggcgaacg ggttaagagt gttttttgat ttccctaagg
     4981 aaggacaagg atgtatcata cccgttgttc tacgagaatt gtttgaagac ggcattgccg
     5041 gaacatggca aaactcagga gagggtgacc gggatggatt ttttatggaa tctgatcgat
     5101 agcatacaaa aaagcgcgat gtatcttgtc attaaggatt tggctccgat tgactgggtg
     5161 gcgatctttt ttgtcctctt tggcatgttg caggggtcac gaaagggttt tatcgatatg
     5221 ctggccaagt cccttaatgt tacgatcgtt attatcctga cattgacttt ttatccgctc
     5281 atcagcgctt atcttgtcaa aaccattcag gcattgccga aggctgtcgc ggatccactg
     5341 gtttttatcc ccaccggagt ggttgcttgg ttggtcaccg gctggtgttt taacgcgatt
     5401 ggcagaattt ttaaggtgga ggtttcttct tttctgaagt tttttggcgg agtttttttt
     5461 ggcggaatct attccgttct tctgctgagt ctgtttgtcc aaatggtcct gttgatcccg
     5521 ggagagatga cgcaaaagcc ttttacccag cgtggatcct attccgggca agcccttgcg
     5581 aagctggcgc ccggtgttca gcgatttgtt gccgctccgt ttgtagggac gcgagagtcc
     5641 cgcagtcgac cggcggtttg atctcatggc acttgatttg ccacgcaatc gggagcagga
     5701 ggatattgtc gagcgtgtcg cggccgcgaa tgacattgtc gaggtgatct cccagtttgt
     5761 tcccctgaaa cgcgcgggcc ggaatttcaa ggcctgttgt cccttccatc aggaaaagac
     5821 tccttcgttc atggtccagc ccgaaaaaca actttttcat tgtttcggat gtcaggcggg
     5881 tggcgacgtg ttcagctttc tcatgaagta tgagaacctt tccttccctg aagctctcaa
     5941 aatgctcgcg gatcgggccc gcatcacgct ccctgaaaaa cgttacgcca agggtgattc
     6001 ctcacataaa gatcaaattt acgaaattta tcagtgcgcg gtggagcttt atcagcggct
     6061 tctcgcgggg gaatcgggaa agaaagcgcg ggactatctc gcgaagcgtg gcatgacgga
     6121 cgaaaccctt cgagagttta aggtcggttg ggcgcctgac gaatggagga cgctctatga
     6181 tcaccttttg aagaaaggtt atccggaggc cctcttgaaa cagtcttcgc tcattcagca
     6241 atccgctaaa ggtaattatt acgacacgtt tcgggggcgc atacttttcc cgattatgaa
     6301 ccttcagaat aaacccgtgg cctttggcgg ccgattgctc ggggacgggg aaggccccaa
     6361 atatctgaat tcttcggaaa atccggtttt ttcaaaacgg cgtgagcttt ttgggctgaa
     6421 cgttgccaaa aggtacatcg atgcgaattg gccgaatctc attctggtcg aaggatatat
     6481 ggattttttg gccctttatc aggcgggatt taagaatacg gtcgcgacgc tcggcacggc
     6541 cctgggggag gatcacgctc gtttgatgaa gcgttttgcc gaagaggtga ttgtggtata
     6601 tgacggagac gatgccggag aatcggcggc gctccggggc cttgaaattc ttctggaggg
     6661 aggggggcag gtcaagctgg ccagtctgcc caaaggtttc gatcctgatg attttgtgcg
     6721 aaaacgagga gcggacgcgt tcagggagct gcttaaaaat gcccaggatt tttttgatta
     6781 caagctcaaa actttgcttg agcgatatcc gaaaaatgac gcgctgggtc tggcgaagat
     6841 cagccgggaa atgcttgaaa cttttcgcaa ggtcaaaaat ccggtgatgc tcgcggagta
     6901 tttcaaacga ctgtcacgtg agatcgggat cgacgaacgt tctctgcgag cggagtttga
     6961 aaatatatca cgccggggct cggatctggc gcgtcggcgc ggtgaaaaaa cagttgaaaa
     7021 gaaaacagct ccggcgacac ctcaggaaga agtgttgctt cttgccttga tgatggaaaa
     7081 cagtaatttt ctcgatgaag cagagcgtca actcaatccc gaaacgatgc gttcaccggt
     7141 cagccgggag cttttcaagt ttcttgttga ttcgggagga gtcgcgaaaa aaaagccgct
     7201 ttcgaccgtc ctgaacaaaa tccaaaatac ggcttaccgg gaacagttgg tgggggcact
     7261 tgccgctctc gaagacacgc cggatcgaaa gcagattttt caggattgcc tgcggacgat
     7321 acggagaaat ggagctctcg accgtcagga gaaactccgg ggcctcattc atgaggccga
     7381 gcgaaacggt gattcggcaa aacttctcgc ttacacccgt gagtttcagg aacagctgaa
     7441 agaaacacgc gagatttgat ttttcaaaaa tgacattccg gacatgatcg ctaaaaagat
     7501 gcttgggagc aaaaaagctc ctgtgttata attccccgat ttttggggaa gcctgaagcg
     7561 gtttcatagg tctgttactc acttttcaac ctctgggaaa ataacggttg ggggtgtctg
     7621 tgcggccgcc cggaggaagt gaattcaatc gtgaaatcat tgatagcccc gatccgttgc
     7681 tggcaacgta atcttcttga tacgcttgtg cgaaagtatt atcaccaggc gcttgaggcc
     7741 cgggcggaag ggatggttcg gctttttgag cgtgacattc ccgagcggtg ccggatcctg
     7801 gacctcggag gtggctgggg gttttacaat gagccgttta aacggcgggg tcacgagtcg
     7861 cttattctgg atgtcgtgaa accgggctat caaaaagcgc ccgttgtgat ttatgacggc
     7921 ggcagaattc cttttgggga tcgcgcgttt gacgtgacgc tgatggcgac gatgctacat
     7981 catgttccgg atccggaatc gatgatggcg gaagttcgac gcgtgacccg gacgaaggtg
     8041 gttgtgatcg aggatcttta tcatcatgcc atcggccggt tgtggacgat tgccagggac
     8101 cggattctca atatggaatt tctgggtcat ccgcaccagt tcaggaaaca cgaggaatgg
     8161 atccagtttt tccgggagaa ggattttcgc cttgaaaaat tccggaagtt ttatacctgg
     8221 atttcggggt ttcgaatttt aaacggtttg tacgtgtggg agtgcggcga aaatgtcggt
     8281 gctatcggtt gaacaagttc tgtggatgaa cgaaggcgaa gggattacgg cgcgcggaaa
     8341 aatccctcat gactcggagt tttttaagga tcatttccct gaatttcccg tactgccggg
     8401 tgtgcttgcg atcgagatgc ttaagcagac cgcggagaaa tatctggaat cgttttccga
     8461 agacggggcc ggagggtctc gttttttttt gaagcagatt cgcgcaaccc gttttaggaa
     8521 gtatcttcgg cccggagatg agtgggaaag ccgattgacg ctcactcaaa gcctggaagg
     8581 tgagacggtg tgggacgcga aactttatta tcacggtgaa gtggcggttt ccgcgaagct
     8641 gacgcttgcg aggatcaaac accctccagt cggcgcgagc gatatccccc taacggaagg
     8701 aaaaggagaa ttatgaatca agaaattttt aagaaagtac aggaaacatt ggccggagcc
     8761 cttggagtag agacctcgga aattaccccc gaatcttccc tgaaacggga tcttggcgcc
     8821 gaatcgattg attttatcga catcattttc cggctggaaa aaagttttga tcttaaaatt
     8881 cccgccaatg atcttttccc aagttatatt tttaccgacg agagattcgt gaaggaaggt
     8941 gcggttacgc ccgaggggat tcaggaattg cgcaccaaac ttccgtactt ggaactcggt
     9001 gattttgaaa aggatccgca ggtcgcggag cttgccgatt attttacggt caagatgata
     9061 ctccggtatc ttgaagagag actgggaaaa tcatccggtt gttcgggcgc ttgtcagtag
     9121 aagtttgccc caacttgatc tatttgccga aaaaattacc tgtgtcgcgt tccccccaag
     9181 aaaaaaacac tccgggatgg atattacgcg gaaggcccaa gaccttacat ggcatcggca
     9241 ttgatattgt ttcccggtcg cgtatgaaga aattcttgtc gcgtcatcgg gagatgatcc
     9301 tgcggcgttt tttgaattcg cgggagcgtg agcagttttc ggagatacgg ctcacgcggc
     9361 tggcggagct ttttacggcg aaggaagctt tttttaaggc atgtaacgag ccctggatgg
     9421 ggcttgaagg atttcaggga atgcgaatta ccaaacgaat cggcgattat tttgaaatgg
     9481 aatggcacgt cgcgggaacc cgcagatgct acaggggcga cggatctttt tttcgagacg
     9541 gggatcttgt cggtgcccag gccatgatct ggagctgacc gtgcgctggc ttcttctgga
     9601 tcaggtggtg gcggtgcatc gtgggaaaaa agccgtaacg ctgtctcgcg ttccccaagc
     9661 tccttattca cgcgaggttc ttttcatcga aatgatggcg cagacgggag gccttttggt
     9721 tggcgcggaa agtgattata acaatgacgt tatcttcgcc aagatcgaaa cggccacttt
     9781 caccgagcct ccggctcccg gaacggcgct tacggtagag gcatccgcgg atggtcttgc
     9841 ccccgagggt ggatggattg agggacagat cacttcttcc ggaggcgttg tcgccaaagc
     9901 caaacttctt ctcatgtccg tggggcggct tatcccggaa agtcaaagat cgatagcgtt
     9961 tcatgataat ttcatgaacc attttaaggt cagggagaag gtgacggtgt catgacgcgc
    10021 cgtcgggtgg tagtgacagg tttcggagtg atgagcgcgt gtggtaaagg atggagtccg
    10081 tattggaacg cggcggttca cgggcaatcc gcgatccgcg aagtggaaca tctcaagctg
    10141 aacggatctt cgctcaaata cgcggggttt atcgcggatt ttgatcccaa acagtttgtg
    10201 accaaccgga aatctttgaa agtgatgtcc cgggaaattc aactggctgt cgccgcggga
    10261 aagctcgcgc tcgaggacgc gaaacttctg gatatggctt acgatcaatc gaggattggc
    10321 gttgtgctgg ggaccggagt tattaataat gaccttgatg agattggagc gggtttcaag
    10381 agcgcgattg acggttccgg ccgtttttca atggcgcaat ttggtcagga cggggttcgc
    10441 tccctttacc ccctgtggtt tcttaaatat cttcccaata tgccggcctg tcacgtttcc
    10501 atcgcgtatc atttgcgtgg ccccagcaac accattacaa cctcctcggc ggcgagtgcc
    10561 caggcgatcg gggaagccta ccgggtgatt gaacgcgggg acgcggacat tatgcttgcc
    10621 ggaggcacgg attcaaaatt aaacgcgatg gggatctccc gctttaatct tctcgggctt
    10681 ctgtccacgc gaaccggagc tccggaaact gtttacagcc ccttcgacga aaagcatgac
    10741 gggattgtct tgggggaagg ggcggggatc atggtgcttg aatcgcttga aaacgcgaaa
    10801 cgtcgcgggg cgatgattta cgcggagatc acggggtatg gatcttcatc ggattttaat
    10861 tttgatccgc gagacgagga cgattttacc gggaaacggg tcgcgatgaa gcgcgcgctt
    10921 gaggattccg gtattgaaca ccacgatgtg gatgttatcg tcgccaacgg aagcggagtt
    10981 cctcaggcgg atattcagga ggccctcgcg atccattcgt tttacatggg atcatttgga
    11041 aaactgaacg tgacggccct gaaacctttg acgggcaatc ttgtttacgc ttcggggggt
    11101 gttgaagcgg tgattgccgt attgattgcc aacaaaaaaa cgattcctcc cgtggccaat
    11161 ttaaacaaac cttaccccga atgtcccttg ccgcttgtga aaggcaaggc ccggtcggct
    11221 gaagttcagt tcgtcatgct caacaatttt ggactgggcg ggcaaaacgc atccctggtg
    11281 ttcaaacatg tcaaaacgta gagttgttgt cacgggagta ggcgcggtga ccccgctcgg
    11341 gaacaatgtc cgcgacacct ggttgcgtgt cacgcagggc aaatccggag tgggaccgat
    11401 cactcttttt gacgcctcga cattcgcgac ccggatcgcg ggtgaagtga agggtttcaa
    11461 ttttgagcgg tggattgaac gtaacccgcc tttggcctca gccgctcgcg gtacgttttt
    11521 tgcgctccat gccgccgcgg aggcgtacaa ggacgcgatg ctaaaacctt tcactgtcga
    11581 ttcccggcga tttggaattt attttggcgg aggtgacagc gggatcgatt tcgatccttt
    11641 cgtcaaaacc cttatggcgt cctttgccgc cagcggggat ctggttgaca agggggaata
    11701 tgtccatcaa tcgctacggc acatgaatgc tcttcgggag ctagaaagtc agccttttat
    11761 gacggtgacg cacctgacgc ggctgtttga tattcggggt cccgtttcca actgcttgac
    11821 ggcctgtgct gcgtcgtcgc aggcgatcgg agaggcttat gagtggatta aacggggaga
    11881 tgccgatgtg atgatgaccg gaggatctca ttcaatgatt tatccgctgg gtgtggtggg
    11941 cttcagtctg ctgaccgccc tctcgacccg taatgacgag ccccagagag cgtcgcggcc
    12001 ttttgaaaaa aagcgggatg gtttcgtgat cgcggaaggc gcgggtgttt tgattctcga
    12061 agagttatct cacgcccaaa agcggaaagc gcacatttac ggcgaggtgg tgggatatgg
    12121 ttcgaccgcg gattcttacc ggctgacgga catggatccc gaagcgcgcg gggcttcgga
    12181 agcgatgcga atcgcgatga caaaggccgg gatcgagcct gccgatgtcg actacatcaa
    12241 cgcgcacggc acctcgaccg gagtgaacga ctcccttgaa accgttgtga tcaaaaaggt
    12301 gatgggagaa tatgctccga aggttccggt gagttcgaca aaatccatgc tgggacacat
    12361 gatcgcggcg gcgggcggag tggaagccat tttttgtctt cttgccatgc gcgactccat
    12421 cattcccgca acgatcaatt acgaggaacc ggatccggag cttgatcttg attatgtccc
    12481 aaatacggcg cggcgagcca gtgtgaatgt cgcgctttcc aactcgtttg gatttggcgg
    12541 ccagaatatc tgtctcgcct tgaggaaata caatggctaa taatcttatt tttatgacag
    12601 gtgcgaccgg ggttctcggg caggaactgg tcaaggagct tttgttaacc accgacgcga
    12661 cgatcgcgat cttgtcccgc gcgaaaaacc gtgtgccgca cgacgaacgt gtccagaaaa
    12721 ttctgaaagc gacggggatg gatgcccatt ttggatctcg cgtgcgggtg ctggagggag
    12781 atgtcaccag gtcaaagttc gggcttaaaa aaagcgatct ggatttgctt gaacgggact
    12841 gcaaggtgtt ttatcacatc gcggccctga cggctttgaa cggcaccgag aaagagtgcc
    12901 aagagatcaa tgtcggagga acaaagaacg cgcttgaact tgcctgggac ctgaggaaaa
    12961 agggatcgct cgaacgtttt gtttatttca gtacggcttt tgtcgccggc agtgtgtggc
    13021 attaccgctc cccggaagat tcgctccctg aaaaacccgc gtttgcgaat tattacgaat
    13081 ccagcaaaca tcaggctgaa ggcatggtgc gcgaagcgat gggccaaggg atgcccgtga
    13141 cgattatccg gccgagcatt gttgttgggc attccgagac gggagaggtt tcggacttta
    13201 atgtcattta tccgttcatt aaactttttg cccacgggat tttgacgaaa ttgcccaccc
    13261 ggcccgagaa cacgtttaat atcgtgccca ttgattttgt gatcaaggcg acggccgcga
    13321 tcatgcgaaa cccccggtcc atcaataagt gctttcacct tgtcgcgccc aatcctccgg
    13381 cgattggcat gcttctttct ctcagagacg cgtttcccaa atccgcggta attgaaatgg
    13441 tagaaccctc gagttttcag aaagcccagc tcgatcccca acagcaaatg gtcttcgcga
    13501 tgctcgagcc ttacctcggt tatttaaacg gcgacctgac ctttgacacg aaaaacacgg
    13561 aagagattct tcgtggaacc gggatcaaga tgcccgtgac ggaccttgct ttcttgaaaa
    13621 cccttattac ctatgccgtg aaatccggct atctggtcat ctgatcacag gagtggataa
    13681 aatatcgtac cgattttggc gcgcggaacg ggcacgaaaa catgaaatga ggatttcgcg
    13741 cgtgacgttg catttggcaa aatcctgagg atggacgggc atgttctggt gttccgtcgt
    13801 cggagagccg ggatgctttg acggggaaga ttgccttttt gaaaaactgg gttgaggatc
    13861 aggaaatgaa agtactgttg attgagccac ctccggcttc aaaattcggt aacttgagaa
    13921 ctctcggaag catcggaacc ctcaaagcgg atatggcatg gccgccgctg gatctgatga
    13981 ttattgccgg gcttctcaaa cagcattcta tccccgtggc tctttatgat gccaattcgc
    14041 tgaaagcgac ctttgaggat gttcagaaaa aaatcgagtc ccaaaagccg gatttggtca
    14101 tttttacgac ttcaacgccc agcctttacc atgatcttca aacggcgcgg gtagcgaaga
    14161 gggtatcgag aaatatcctg acggcggtga cctcgaccca tgtcaacgcg ctcccgaaag
    14221 aaaccctggc gctgagcgat gacctggatt tttgtatccc taacgactcg gaaggaacgg
    14281 tcctggacct gatacaaaaa gacggccggc cggaagatgt cttgggcctt acttaccgca
    14341 aagatggaaa aattatccag aacgcgcccc ggccggtttg taccaatctg gatgaacttg
    14401 gaattccggc ccatgatctg atcgatcttg atatttaccg ggacccgttc acaaaacgga
    14461 tgccgatggc cgtgacctac acatcccgag gctgtatcaa ctatccgcct tgcgtgatgt
    14521 gctcggcctg tttttataac cgggtccggt atcggtccac ggaactggtg atgcgggaat
    14581 tacgctggct gaagagcctc ggggtccagg aactgagatt cccttttgag gcgggattca
    14641 acgacaaccg gcgggcccat gagctttttg acgccatgat tcaggaaaaa cttggcatga
    14701 cgtttacctg caacggccgg gccgactgtc tttcggagga aaaagttcgt cagatgaagg
    14761 ccgcgggatg cagggccatc aacattggtt gtgaaagtac tcatcccgag accgtcgaat
    14821 ttatcaagaa aagagtctcc gcggagcagg tcgtcagggc ggtggcgatg gccaaaaaag
    14881 ccggactgga aaccctggtt tattttttgt ttggtttccc ttatgaaacg aaagagagca
    14941 tgaaaaaaac gctcgagttc gcgaaaagac tggatgcgga tctggtgact ttcggcatcg
    15001 caatccctca tcccgggacc gaattttaca ggtatctgaa ggatcatggt cttttgtctt
    15061 ccgatcaatg ggatctttac gacccgatgt ttcctccgcc ttacgcttat cccgaattat
    15121 cgtcccggga gatctttgat tttgcccgat acgcctaccg ttcttactat ttgaggccca
    15181 ggtttatcct gaaacgattc ctgaagttta atcttgtccg cgaaacaaaa aattttctcg
    15241 ggttcatgaa ccgctatgtg acaggatcat gacccggtta ttgcttcttc atccatccgg
    15301 aaaacttctc gggcgaaagg acaatctcta caccaacgaa tccctggtgc catccctcgg
    15361 acttgcgagt gtcgcggcct actgccgggc ttgtggtatt gagacccgga tctacgatct
    15421 ccgtctcgaa aacaacggaa tcgaaggggt ttttgagcga atcgacgact tgaatcccgc
    15481 cctcgtcgga attaccgcat ttaccaccga aattacgtcc gcgggcgttc tggcgtccag
    15541 gatcaaacag aagtatccgg atcttcagat cagtgtggga gggccgcacg gtaccgcaat
    15601 gccccaggaa acactgttgg aatttgaggc gtttgattcg gtagtatgtg gtgaagggga
    15661 aaagaccacg gcggaattag tggtggctac ccaggtgggg ggatgccctg atctttcgaa
    15721 gatccccgga ttggtttacc ggaaagaggg caagattttt tcgaacccaa cgagggagcc
    15781 tgtggctgat ctggacactt tgccgtttcc cgcgtgggat ctttttgagc ttgaacgcta
    15841 taacggaatt ttccctctca gcgcgtctcg cgggtgtccc taccgctgtt atttttgcac
    15901 gcctaactat cttggcaaaa aagttcgtgt caagagtcac caaaaaatca tggaggagat
    15961 cacctggctt gtcgaacggt ttggcgcgaa acgtatccag ttcacggatg ccatgatggg
    16021 tttgcttaag gatgaatcga tccagatgtg cgacgagctg atccgtagcg gacttggccg
    16081 agagatacaa tgggattgtg agactcgcgc ggatgccgtc agtctcggtc tttttcagaa
    16141 aatgaaggag gccgggtgcg agtgggtcgc gttgggagtg gaaagcggca acgagcggat
    16201 tctcggggaa gtgattaaaa agggagagac caaggatcag atccgtgttg cggtccagct
    16261 cgcgaagcgg gcgggactca aggtgaggac cttttttatt ttgggacatt acacggaaac
    16321 gcccgaaacg attcgcgaga cgatccgttt cgcgcttgag ctcaatccgg acgcgatcgc
    16381 gtttggattg atcgtgccga atccgggttc cgaattcaga aaaattgcgg aaaccaaggg
    16441 aagcggtttc agaattcttc acaatcggtg ggaagattat cagcagttta actataactg
    16501 ccttgagtcg gaaaccttct ctctggcgga attgaaacgg tggcaggcga gggcctactt
    16561 tactttttac gcgcaccatc cgataaaagg tttttttctg ttttttgccc agtcggcgta
    16621 taactacagg ctatcaggtc ttttgaagct tcctcttatg cttctgcgaa atttgttggg
    16681 gaaatcctga tcaaattgat cgtggacgcg aagcctggca gaggaaaagc gatttaaaaa
    16741 atcggaacac aaaaagctga tgacacattt tgggccttat gacaaaatac gtcatttgaa
    16801 tgtccgggac gtttcggtcc cattcttcaa ccggaaattt acgagctatg actgagtcgc
    16861 ttccacaaaa gacacgagtg gctgtcgttc ttccctgtta ccgggttctt cctcatatcc
    16921 agaaaacact cgcggggata ggtccggaag tctggcgaat ttttgtcgtg gatgacgcct
    16981 gccctgatca atccggagat tttgtcaagc gacattgccg ggatccgcga atcgaggttc
    17041 tttaccacga caaaaaccag ggggttggcg gggctgtttt gactggctat caggccgctc
    17101 tggatgccgg ggccgatatt atcgtgaagc ttgacgggga cggacaaatg gacccggggc
    17161 ttgtgacgga cgtgatcgcg cccattgtgg ctggggaggc cgattacacc aaaggtaacc
    17221 ggttctttga tcccgagggg gtccggacca tgccgcgtct ccggctgttt ggaaacgcgg
    17281 tgctttcctt gatgacgaaa ctttcgtccg gctactggaa tctgtttgat ccgaccaacg
    17341 ggtatacggc gattcacgcg cgggtcgcgc gacggcttcc gtttcacaaa atcagtaaag
    17401 gatatttttt tgaaacggac atcctttttc gtcttaatac gttgcgggcg gtcgtgatgg
    17461 atgttccgat gaacgcgaga tacggaaccg aaaaaagcaa cctgaaggtc tcgagaattt
    17521 tgggagaatt tttattcaag catctccgta attttctgaa aagaattttc tacaattact
    17581 atcttcggga catgtcgttg gcgtctctgg aacttccgct cggagtgttc ttttttttct
    17641 ccggcggttt ctacggcgcc tgtcactggg cccggtcctc gcaggccg
//
LOCUS       DAOSIO010000011        16371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_3719_length_16371_cov_4.708446, whole genome shotgun sequence.
ACCESSION   DAOSIO010000011 DAOSIO010000000
VERSION     DAOSIO010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 16371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16371
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3719_length_16371_cov_4.708446"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="PK590_00950"
     CDS             complement(<1..990)
                     /locus_tag="PK590_00950"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG23196.1"
                     /translation="MRILIITQYFWPENFRINDLAKGLVEKGHEVVVLTGIPNYPQGD
                     FYDGYGYFKNLRQEYQGVKIFRVPLVPRGNGGKLRLTLNYLSFAFCASLWGPFLCRGK
                     FEAIFVFQTSPVTVGIPAVVLKKLKSAPLLFWVLDLWPESLEVVGFCRSSWGVTLVDV
                     LVRWIYRSCDKILVSSKGFVASIQARGVSEEQILYFPNWVEPVYREISLKEKTGKFFE
                     DLPRGFRVLFAGNIGEPQDFGTIVAAAERLKPYHDIHWIIAGDGRMAAWAKTAVSSRG
                     LATHFHFIGKYPSEKMPELFSLADVMLVTLKKDPGLSTFVPGKIQSYLACGKTI"
     gene            complement(1111..1239)
                     /locus_tag="PK590_00955"
     CDS             complement(1111..1239)
                     /locus_tag="PK590_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23197.1"
                     /translation="MNEIIALLIVAAAVIILLVRFFKTGGGGCRNCGKTCFPADKK"
     gene            complement(1342..3357)
                     /locus_tag="PK590_00960"
     CDS             complement(1342..3357)
                     /locus_tag="PK590_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOG23198.1"
                     /translation="MSHKNGKKTKEIVLVGNPNTGKTTIFNALTGLRHTTGNYPGVTV
                     EKRAGSMTLADGRKVSVIDLPGAYSLQPRSPDEQVVHHVLFGAQKGMPPPDLVLVILA
                     ANNLERNLYLATQTADLGLPTIFVINMWDIARETGLEIDLVKLEAMTGVRCVPAAGMQ
                     GTYIKKLKRVIWKELKKKSSEKASVSFPGDTSKRVFVAPEIRYRMIENIVHAVVRRAE
                     GETCGVSEKLDRVLTHRVWGLLAFMGVMAFVFQSIFSWAQIPMSWISRTIERFSVFLE
                     KVLPDGALQSLLVDGVIAGVGNVVVFLPQIMLLFFFIAFFEDTGYMARAVFVLDRVMK
                     TVGLNGKSFLPLLSSFACTVPGVLATRTIEDRNDRLATILVAPLMSCSARLPIYTLLT
                     AAFIPSQFFFGFISLQGVTLLAMYLLSIGTGLGMAAIFRKTFLKGNRMPFVFELPPYR
                     MPNLANVFAVMWDRSKEFLVRAGSVIFLFSIVLWFCVSYPKNPAAEARFESARRQALS
                     ELSGEALDTKLEEIERDQKAIQIQSSYAGSLGRMIEPVIRPLGFNWEIGIGIVASCAA
                     RELLISTLAIVHHARSEGEGVTAGLIHALRSAEDPTTGGALYTPLVAVGLMVFYVFAC
                     QCFSTYVILKKETGSWGWTFFTIFYMTVLAWGGAFIVYQGGRLLGFQ"
     gene            complement(3440..3751)
                     /locus_tag="PK590_00965"
     CDS             complement(3440..3751)
                     /locus_tag="PK590_00965"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOG23199.1"
                     /translation="MNLVTLDRMLPGEGGRVHGYQEPKELHHRLKELGLVEGTRLRVR
                     RCAPLGDPMELSIRGYHLSIRKEDARHILVIRDEGPCGCENRRGHRGGQEWRGKHQIS
                     K"
     gene            complement(3748..4179)
                     /locus_tag="PK590_00970"
     CDS             complement(3748..4179)
                     /locus_tag="PK590_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOG23200.1"
                     /translation="MKSKRKRDFSKFLRQKSYRVTAERLRLLEGVRRQRGHFNVDELV
                     RRLHDQGLKVSRDMVYRNIPILLESGVIEQSFKTSRDTFYESAYQKKHHDHILCRQCG
                     KVAEFEDTDIEKLQEIIAQRVGFKLEYHCHQLVGLCAKCRK"
     gene            complement(4349..4612)
                     /locus_tag="PK590_00975"
     CDS             complement(4349..4612)
                     /locus_tag="PK590_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23201.1"
                     /translation="MQCPLSGCFFFSPSVIFFIVAALSLGLGLLSILKPERSILLYQK
                     IMKYFNWKVEPIDPARELRGTRTLGGWLTVLAVLLGMVTLLKF"
     gene            complement(4788..6824)
                     /locus_tag="PK590_00980"
     CDS             complement(4788..6824)
                     /locus_tag="PK590_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HOG23202.1"
                     /translation="MAHTYRDHPDPQKRKAPPKKKLEKPEEREIWVGDTAVIERHFLH
                     LNKLLRAEERSEQDRFQRDFIDQKPEDRERGGKAIYRLELLDVHYNPSGQKLLTFALK
                     TKRPLPRYSLAVGDIVRLSGFQTPEADCPVGAVYERDRTRITVAFSGKLPAWVDRENS
                     FQLSVAANLSTYERMREALRLVKEADHSRIAVLRDISLGLRTPKFDDPVSVTKFPFLD
                     STLNEIQQKAVCMAHEAKDVLLVHGPPGTGKSRVLIEIIRQAVARSETVLVSAPSNAA
                     CDHLVECLAAHKAPVTRLGQPARVSERIREHTFSYKLAAHPFAKMIEENEARLEQISR
                     QKERRQVRRIIEWEERQEMRTEIEQIRKDIRDLRAQIFKQVWNSSDIVVATHVGAADP
                     IIKAKSFDWVIIDEATQGIEPSTWIPMLYAGKVIMAGDHCQLPPTVHSFFKGREGSLA
                     LTLFERFYEKLGDDSKIRLERQYRMHENIMNFPSKKFYDGKLVADERVRCHQLTDLPF
                     VNGKGLDLTPAIFLDTAGLGYEEEGEEGTGSWSNPQEAKRVVRELDKILKAGVRPADI
                     AIISPYSAQVKLLTGMMMGDSQDPGEIRELEIDSVDAFQGREKEVVIVSLVRSNSEGE
                     VGFLADTRRMNVAITRAKRKLILVGDSATLCRLPFYDDLLKYMETVGGYQSAWE"
     gene            complement(6933..7349)
                     /locus_tag="PK590_00985"
     CDS             complement(6933..7349)
                     /locus_tag="PK590_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOG23203.1"
                     /translation="MTAHTEYLWMNTKDRYAIVNITDEVESVIQKSGVKEGLCLVNAM
                     HITASVFINDHEPGLHRDFLNWVEKLAPYGRDRYQHNLTGEDNGDAHLKRTIMGREVV
                     VAITDGKLDFGPWEQIFYGEFDGQRKKRVLVKIIGE"
     gene            complement(7558..7914)
                     /locus_tag="PK590_00990"
     CDS             complement(7558..7914)
                     /locus_tag="PK590_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase system-associated protein, TIGR04073
                     family"
                     /protein_id="HOG23204.1"
                     /translation="MKKILLAFVILGLATGTSAIAAEKLQADNAASKLARGATNIATC
                     WGEYITQVPASVEKSPDYMTALFYHVLRGTVFTVRRAAVGVYDVVSFPFPGNNCYGPV
                     IEPETIFTPTVEVLSN"
     gene            complement(8145..8696)
                     /gene="amrA"
                     /locus_tag="PK590_00995"
     CDS             complement(8145..8696)
                     /gene="amrA"
                     /locus_tag="PK590_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOG23205.1"
                     /translation="MMLSQSEQQKTLRYINDLLKARLTHGPEPELDLSEEFLEKKRGI
                     FVTLKRKGMLRGCIGQIIGHEPLRESIRCMALAAAFEDPRFPPLALSELDGLHIHVSL
                     LTEPASICSYKDIRVGIDGIIVSCSWKKGVYLPEVAIETGWDAKTFFESCALEKAGLD
                     ETELSGAKIEVFQTEGFGGDGNL"
     gene            complement(8742..9584)
                     /gene="amrB"
                     /locus_tag="PK590_01000"
     CDS             complement(8742..9584)
                     /gene="amrB"
                     /locus_tag="PK590_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOG23206.1"
                     /translation="MSHEKPIIRKGIVAGQFYPDRPDVLRHEVRTFLDHASSRPDPSV
                     RGLIVPHAGYMYSGGIAAEAYRAIDGAQAEVIVVAAFLHRIFIQGVFIDKADFYETPL
                     GRIPVNKTLAEKIRDASPYLADTISGDLNEHSLEVQLPFLQMAVKDFTLLPVYIGEPS
                     VANAEALSEALSRELAGKRALFVFSTDLSHFHPYKEAVDMDHKLIRMIGDRDVRRLER
                     SNYAGEIEACGFGPVITSLFLAEKLGWEGPRLLKYANSGDITGEKGSVVGYAAMAYKV
                     NPEA"
     gene            9820..11106
                     /locus_tag="PK590_01005"
     CDS             9820..11106
                     /locus_tag="PK590_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOG23207.1"
                     /translation="MLNRFFKLRERHTTLGTEVVAGTTTFLAMAYIILVNPAILASTG
                     MDKTALILVTCLATALATLATGLFANAPIAMAPGMGLNAFFAYSLVLTDHLTWQTALG
                     VVFLSGLFFFLLTAVGIRQKIVEAIPRSLISAVSAGIGLFITFIGLVNLGIIQKNEVT
                     LVAAAPITPTVMVGLAGFLLMLLLEMHRIKGAMILGIMMSTLIALWTGLVPWPKEFVT
                     FHFDLSPIAFKLDILAALKGGLFGAIFSLMFMHLFDSIGSLVACCHQAKRVDEKGRLI
                     GLDRLLSIDALASMAGAILGTSTITAYVESTTGIGQGGRTGLTSVVTALLFVLAIIFV
                     PLISVVPVYATAPALILVGFFMIKEVREINFEDVEEGFPAFVIIVMIALSYSIATGLA
                     FGFLSYVLFKILRLKFAEVKPVMWVIAGLLCLHFIV"
     gene            complement(11136..11681)
                     /locus_tag="PK590_01010"
     CDS             complement(11136..11681)
                     /locus_tag="PK590_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23208.1"
                     /translation="MKRFLCCFVLILSLSFSVSAEEPAVRERIIGSAFKGLANAYLAS
                     CDFKKEKQRYIERLDKTSDEDFMVRYGSYWLLIEKLPVRIKIENGFSQTMTREQLISR
                     LREIRREDLQEIINGVPDSAISKEFDRRIKRLGKKVEKNDLAQQIQILWQRILKKAGQ
                     SGEKTHKVSRPQGKGNSPTLN"
     gene            11844..12908
                     /gene="amrS"
                     /locus_tag="PK590_01015"
     CDS             11844..12908
                     /gene="amrS"
                     /locus_tag="PK590_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOG23209.1"
                     /translation="MNRTPDALYWKDLPDGRIECALCPRRCKLFDQQRGFCFVRVREG
                     NRMVLATYGRTSGFCVDPIEKKPLFHFLPGSSILSFGTAGCNLGCLFCQNWNLSKSKG
                     TDTAAEQASPEEIAKAAKSARCESVAFTYNDPVIFHEYAVDTAQACREHGIKTVAVTA
                     GYVSPEPRREFYKWMDAVNVDLKGFTENFYKKYCRASLGPVLETLEYIKKETKTWLEI
                     TTLLIPGLNDSEKELHDMSSWIVKTLGPDVPIHFTAFHPDYKMNGISPTPPETLQKAR
                     TISRDHGIRYCYTGNVRNPDGESTFCHHCGKKIIGRNWFKLTEWNLKDGSCRFCETRC
                     PGHFEDGPGRQGDQGKFIPF"
     gene            complement(12963..13523)
                     /locus_tag="PK590_01020"
     CDS             complement(12963..13523)
                     /locus_tag="PK590_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOG23210.1"
                     /translation="MKCRIVEIKARCSDVSRIREILKSRRADFRGTDHQIDTYFRVPR
                     GRLKLREGQIENALIFYQRSNQKKSKRCDAFLYQCPEPDLMKKILNVAFGVLAVVDKM
                     REIYFIKNVKFHIDQVKGLGRFVEIEAQGRTPECRITELRRQCDHYRKLLGIAPEDLL
                     ADSYSDQLICKRRNRGTTCLRGRRGR"
     gene            complement(13679..14524)
                     /locus_tag="PK590_01025"
     CDS             complement(13679..14524)
                     /locus_tag="PK590_01025"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOG23211.1"
                     /translation="MKFLGKILIFIVAVFVVVVLARNVIVKAAVELGAKAAVGLPVNI
                     KMLDIGLQQTYLDIEGLNVGNPEGFGDETMVDVPKVFVDYHLSDIMSGKLHLEKVEFD
                     LRQFTVVRNRDGKLNINSIKGIEQDKTSSGKPETPKKETKAMPIQLDHVHLKIGKVVF
                     IDRSGAQESVREFNINLDESYENITEQGALIRVIVLRVMTKTPLAMLTNFDLGSLQSS
                     VGGIVGSATDLASQTAAKGLDTIKTTTSMAGSLAGDAKDTVKDAAGTVKSIAGKLKNP
                     FGSGN"
     gene            complement(14582..15283)
                     /locus_tag="PK590_01030"
     CDS             complement(14582..15283)
                     /locus_tag="PK590_01030"
                     /inference="COORDINATES: protein motif:HMM:NF015142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOG23212.1"
                     /translation="MTIKRNHNKRRALKIPVILGIVLLVTAGWFVVHQLAGKNVFCSF
                     SHKMRPTDGVLLYVAPPHSVSAKQHNFHVVSPGVWRSAQPSKESLLRMKAHGLKTVVN
                     LRSGRAIERWERDTTESLGIQHYHFPMSAREEVPIKTIDAVLSVISDTAKQPALVHCR
                     AGKDRTGLMIAAYKISQNPQASFRDIYQEMLMYGYDEKKYPAILTSLRKWAESRGYAE
                     IARAIEFQEKAFLKK"
     gene            complement(15449..15697)
                     /locus_tag="PK590_01035"
     CDS             complement(15449..15697)
                     /locus_tag="PK590_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23213.1"
                     /translation="MFIFHPSETYRPVLPGILPKNKPCLMCDVASWSGTWVFSMLNPM
                     RDSRFMDLSDIIMIGARSDCCVTFCCIPLCLVEWLDSL"
     gene            complement(15794..>16371)
                     /locus_tag="PK590_01040"
     CDS             complement(15794..>16371)
                     /locus_tag="PK590_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HOG23214.1"
                     /translation="EDIPVSEVAYQIALSLRDEIRDYEANGIKIVQVDEAAFREKAPV
                     KKRNWPDYFDWAVKSFNLTVNTDPITQIHTHMCYSHFGEIMQYIMRMDFDVISIEVSR
                     DKGEDILRSFGRADFKRQIGLGVWDIHSPQVPSVAKMEELAKRFLEKIPPENFWLNPD
                     CGLKTRGWDETRQAIFNLVETARTLRDQFKK"
BASE COUNT         4075 a         4516 c         3700 g         4080 t
ORIGIN      
        1 tatggtctta ccacatgcta aataagactg tattttccct ggaacgaatg tcgacaaacc
       61 aggatctttt ttaagggtca caagcatcac atcagccaga gaaaaaagct ccggcatttt
      121 ctccgaagga tatttcccta tgaaatggaa atgtgtcgcc agtccccgcg aactgaccgc
      181 tgtttttgcc caagcagcca tacgtccgtc ccctgcaata atccaatgaa tatcgtggta
      241 cggtttcaat cgttcggccg cggcaacaat tgtgccgaaa tcctggggtt ctccaatgtt
      301 cccggcaaac agaacgcgaa accctcgagg aagatcttca aaaaattttc ccgttttttc
      361 ttttagagat atctcccgat acacgggttc cacccaattg ggaaagtaca aaatctgttc
      421 ttccgatacc ccgcgggcct gaatacttgc cacaaatccc tttgaggaaa ccaatatctt
      481 gtcacatgac ctgtaaatcc agcgcaccaa aacatccact aacgtcactc cccaagaaga
      541 cctacagaaa cctactacct ccaaactttc aggccacaaa tcaagaaccc aaaacaaaag
      601 tggcgcagac ttcaactttt ttaataccac agccggaatc ccgaccgtaa caggagaagt
      661 ttgaaaaaca aagatcgctt caaattttcc acggcacaag aaagggcccc ataaactggc
      721 acagaaagca aacgaaagat agttaagagt aagtctcagc ttgccaccat tcccccgggg
      781 gacaagcggt acgcgaaaaa tttttacccc ctgatactcc tgcctaagat ttttaaaata
      841 accgtaccca tcataaaaat caccttgagg atagttcggt attccggtaa gcaccacaac
      901 ctcgtgccct ttttcgacaa gtcccttcgc aagatcattg atgcgaaaat tttccggcca
      961 aaaatattga gtgatgataa gaatacgcat taaaattcac ttttccttaa aaaattcgga
     1021 tcccgaaccc ctaagatgtt ccgaaatatc ttacggctct ttgccgatat tttttagcct
     1081 gccttagtaa gaaaaaaccg atggttaata ttatttttta tcggcgggga aacaggtttt
     1141 tccgcagttt cggcaacccc caccgcctgt tttaaaaaag cgaaccagga gaataataac
     1201 ggccgcggca acgatcaata acgcgataat ttcattcatg tccataaata cctcgtggat
     1261 acaagtcgag gatagcggat tgtttaaact ccgaagctcc atccttcccc gtcatccgca
     1321 tccgtcctcc tttcccgtct tttattgaaa tcccaataac cgtcctccct ggtagacaat
     1381 aaacgcgccg ccccaggcaa gaacagtcat gtaaaaaata gtgaaaaaag tccatcccca
     1441 ggaacccgtc tcttttttga gaatgacata cgttgaaaaa cactggcacg cgaacacgta
     1501 aaacaccatc aaccccacag ccaccaaagg ggtataaagc gctcctccgg tcgtgggatc
     1561 ctccgccgac cgaagggcgt gaataagtcc ggcggtcact ccttctcctt cacttcgcgc
     1621 gtggtgcaca atcgcgaggg tgctaatcag caattcgcgg gccgcgcacg aggccacaat
     1681 cccaattccg atttcccagt taaaaccgag cggacggatc acaggctcaa tcattcgccc
     1741 aagagatccc gcgtagcttg actgaatctg gatcgctttc tggtcgcgtt cgatctcttc
     1801 aagcttcgtg tcaagcgcct ccccgctcag ttcgctcagg gcctgccttc tggcactctc
     1861 aaaccgcgct tcagcggccg gatttttggg ataactcacg caaaaccaga gcacgatcga
     1921 aaaaagaaat atcacgcttc cggcccgaac caaaaactcc ttggaccggt cccacataac
     1981 cgcgaagaca ttcgccagat tcggcatccg gtacggcggc agctcgaaga caaacggcat
     2041 ccggttccct tttagaaaag tcttccgaaa aatagccgcc atcccaagtc cggtcccgat
     2101 acttaaaagg tacatcgcga gaagggtcac tccctgaagc gagataaatc caaagaagaa
     2161 ctgggacgga ataaaagcgg ccgtcaataa cgtgtaaatc ggaagtctcg cgctgcaact
     2221 cataagcggc gcgaccagga tcgtcgcgag ccggtcgttt ctgtcctcga tcgtccgggt
     2281 agcgagcact ccgggtaccg tgcacgcgaa ggaacttaac agaggcaaaa acgacttacc
     2341 attcagaccc actgttttca tgacacggtc cagcacaaag accgcgcgcg ccatgtaacc
     2401 ggtatcctca aaaaaagcga taaaaaagaa aaggagcatg atttgcggca aaaaaaccac
     2461 gacgtttccg acgccggcga tcacgccgtc cactaaaaga ctttgtaacg cgccgtccgg
     2521 caggactttt tccaaaaaaa ccgaaaacct ttcgatggta cgggaaatcc agctcatggg
     2581 aatctgggcc cacgaaaaaa tcgattgaaa aacaaacgcc atgaccccca taaaagcgag
     2641 aagtccccag acccggtgag tcaaaactct gtccagcttt tcagaaacgc cgcatgtttc
     2701 gccttcggcc cggcggacca ccgcgtggac gatattttcg atcattcgat accggatctc
     2761 gggagcgaca aaaactctct tcgacgtgtc cccggggaat gacacggacg ctttctcgct
     2821 tgattttttt ttgagttctt tccagataac ccgcttgagt tttttgatat aagtcccctg
     2881 cattcccgcc gccggaacac agcggacacc cgtcatcgcc tcaagtttca ccagatcaat
     2941 ctccagcccc gtttcacgcg cgatgtccca catattgatc acaaaaatgg tcgggaggcc
     3001 caggtcggct gtttgagtag ccaaataaag attccgttca agattattcg cggcaagtat
     3061 cacaagcact agatccggcg gaggcattcc cttttgcgct ccaaacagca cgtggtgcac
     3121 gacctgctca tccggtgatc gcggttgaag gctataagct ccgggaagat cgatgacgga
     3181 caccttgcga ccatccgcca gcgtcatgct ccccgcccgt ttttccacag tgacgcccgg
     3241 ataattgccc gtcgtgtgac gaagccccgt cagggcgtta aagatcgtcg ttttccccgt
     3301 gttgggattt ccgacaagta cgatttcttt agtttttttt ccgttcttat gactcatgtt
     3361 tttatatttc agctcgcccc tttgccggtt ttccggaaag ggaccggaac acacgccgcg
     3421 gaggaaattt ttgcctttgt cattttgaga tttgatgttt gccgcgccat tcttgccccc
     3481 cgcggtgtcc gcgcctgttt tcacaaccac atggtccctc gtccctgatg acaagaatgt
     3541 gcctcgcatc ctccttgcga atagaaagat gataaccccg gatggacagt tccatcggat
     3601 ccccgagagg cgcgcaccgc ctcactcgca aacgtgtccc ctcgacaagc ccaagctcct
     3661 tcagccggtg atgcaactct ttgggctctt ggtagccgtg gacccgcccc ccttcgcccg
     3721 gcaacattct gtccaatgta acgagattca tttccggcat ttcgcgcaaa gccccacaag
     3781 ttgatggcag tgatattcca gtttgaaacc tacccgttga gcgatgatct cctggagttt
     3841 ttcaatgtcc gtgtcctcga attccgccac ctttccgcat tgccggcaca aaatatgatc
     3901 gtgatgtttt ttttgatagg cggattcgta aaaagtgtcc cggcttgttt taaagctctg
     3961 ctcaatcacg cccgattcaa gaaggatggg gatgttgcgg taaaccatgt cgcgagaaac
     4021 cttcagtccc tggtcatgga gacgtcgcac cagttcatcc acattgaagt gtccgcgttg
     4081 ccgcctgact ccttcaagaa gacgcaacct ttccgcggta acgcgataac ttttttgcct
     4141 caaaaattta gaaaaatccc ttttcctttt tgatttcatg ggaatccctt ctggagctta
     4201 tcaagactta ttcttgataa ggaatatagc agaacggata tggggcgtca atcaatgaac
     4261 gggaaggcaa acttcaaagc taaaagaagg cggagaaaca gcgaatcaaa tttacggcta
     4321 ttttcttgtg tttacgcggt ctcaaaacct aaaactttaa gagagtcacc atgcccagta
     4381 gcaccgccaa tacggtcagc cacccgccaa gcgttcgggt gccgcggagc tctctcgccg
     4441 gatcgatcgg ctcgactttc cagttaaaat atttcatgat tttttggtaa agcaagatgg
     4501 accgctccgg tttcaggatc gacaaaagac ccaacccgag ggacaaagcc gcgacgatga
     4561 aaaaaatcac ggacggcgaa aagaagaaac aaccggacaa gggacattgc attaaaaacc
     4621 tccttttaag ttaaacgcgg gccggaaacg cttccacaag tgtttccacc ccgttgattc
     4681 tcgaaagatc agaaatctcg aaaggggccc atgaagccgc ttctggagag cccggggatc
     4741 aggaaaaatc cggcccgctc tgattttctt tatcctctat cgcccgctca ctcccacgcg
     4801 ctctggtaac cgccaacggt ttccatgtat tttaaaagat cgtcataaaa gggcagacgg
     4861 caaagagtcg cgctgtcgcc cacgagaatc agtttgcgtt tcgcgcgtgt tatcgcgaca
     4921 ttcatccgcc gcgtatccgc caggaatcca acttcccctt cggagtttga ccggacgagc
     4981 gagacgatca cgacttcctt ttcacgaccc tgaaacgcgt ccacggaatc aatttcaagc
     5041 tccctgatct cacccggatc ctggctgtct cccatcatca tgccggtcaa caatttaacc
     5101 tgggcgctgt aggggctgat gatggcaata tcggccggcc ggactccggc tttgaggatt
     5161 ttatccagtt cgcgaacaac ccgtttcgcc tcctggggat ttgaccagct gccagtccct
     5221 tcctcgcctt cctcctcata acccagtccc gcggtatcaa gaaaaatcgc cggagtaagg
     5281 tccaggccct ttccgttcac gaaaggaaga tccgtgagct ggtgacagcg aacccgttcg
     5341 tcggccacta attttccgtc ataaaatttt ttcgacggaa aattcatgat gttctcgtgc
     5401 atgcgatact gccgttcaag ccggattttg ctatcatcac caagtttttc ataaaaacgt
     5461 tcaaaaagcg tcagggccaa ggagccctct cttcctttga aaaaagaatg aaccgtcggc
     5521 ggaagctggc agtgatcgcc cgccataatc actttccccg cgtaaagcat cgggatccag
     5581 gtcgagggct caatcccctg ggtcgcttca tcaataatga cccagtcaaa tgacttggcc
     5641 ttgataatcg ggtccgccgc gcccacgtgc gtcgctacca caatgtcgga cgagttccac
     5701 acctgtttaa aaatctgggc ccgtaggtcc cggatgtctt tacgaatctg ttcgatctcc
     5761 gtccgcattt cctgacgttc ttcccattca atgatgcgac gaacctgccg ccgctccttt
     5821 tgtctggaaa tctgttcaag tcgcgcctca ttttcctcga tcattttcgc gaagggatgg
     5881 gcggcaagtt tgtagctgaa cgtgtgttcg cgtatccgtt cactcacgcg agccggctgt
     5941 ccaagtctcg ttaccggagc cttgtgggcc gcgagacatt ccaccagatg atcgcaggcg
     6001 gcattgctcg gcgcggacac cagaaccgtt tcactccggg cgacggcttg ccggattatc
     6061 tcgatcagta cccgggattt ccccgtcccc ggaggtccat gaacaagcag aacatccttc
     6121 gcttcatggg ccatgcaaac cgctttttgc tgaatttcat tgagggtcga atccaaaaag
     6181 gggaacttcg tgacggacac ggggtcgtca aacttcggcg ttcgcaaacc gagactaatg
     6241 tcacggagaa ccgcgatccg ggaatggtcg gcctctttga cgagtctcaa ggcttcccgc
     6301 attcgttcat acgtggacaa attggcggcc acggaaagct gaaaggaatt ctcgcgatca
     6361 acccaggccg gcaattttcc gctaaaagcc accgtgattc gcgtccggtc tctttcgtag
     6421 accgcgccca cggggcaatc cgcctctggt gtttgaaatc ccgaaagtcg aacgatatcc
     6481 cccacggcca aactgtacct cggcaaggga cgttttgttt tcagcgcgaa ggtcagcagc
     6541 ttttgcccgg aaggattgta atgaacatcc agcagctcga gccggtaaat ggccttgccg
     6601 ccccgctccc tgtcctccgg tttttgatcg ataaaatccc tctgaaaccg gtcctgttcg
     6661 gagcgttcct cggcgcgaag cagtttgttt aaatgaagga aatgccgttc gatgacggcg
     6721 gtgtcgccca cccagatttc acgttcttcg ggtttttcca gctttttttt aggaggggct
     6781 tttcgctttt ggggatcagg atgatcgcga tacgtatgag ccatttgtca tctttcggat
     6841 ttaagtccaa aattcaaact ggtttcggaa tggatgatcc gcctccggct ccgttgggtg
     6901 acaggtcggg aatttcagac aaattcctga aactattctc caatgatctt tacaagcacg
     6961 cgctttttcc gctgtccgtc aaactcgccg taaaaaatct gctcccacgg gccaaaatcc
     7021 agttttccgt ccgtgatggc cacgaccact tcgcggccca tgatcgttcg cttaagatgc
     7081 gcgtccccgt tgtcttcccc agtcaaattg tgctggtacc tgtcccgacc gtacggcgcc
     7141 agtttctcaa cccaattgag aaaatcccgg tgaaggccgg gctcgtgatc attaatgaaa
     7201 acgctcgccg taatatgcat ggcatttacg agacaaagcc cctccttcac cccgcttttt
     7261 tgaatcaccg attcaacttc atccgtgata ttcacaatcg cgtagcggtc tttcgtattc
     7321 atccagaggt actcagtgtg agcggtcatc acaaaatctc ccgtcttggg gggcattcta
     7381 tcacaaaagc cggcataaaa acgttcgggg tggacgccgc tcaactgccc tgggtactcg
     7441 ccgtgaggat tatctcgggt cgtgcccaaa cggggaaata caagcgttta tgaatttagg
     7501 cgtccgcccg aaagtgagag cgacgaatta aattcgtaac tgattttttg tattcgccta
     7561 gttcgacaag acttccacgg taggagtgaa aatggtttcc ggttcaatca ccgggccgta
     7621 gcagttattg ccgggaaacg ggaaactcac gacgtcataa accccgaccg ccgcccgacg
     7681 aaccgtaaaa accgttccgc gaaggacatg ataaaaaagg gccgtcatgt aatccggact
     7741 cttttcaacg gaagccggga cctgggtaat atactccccc cagcaagtcg cgatattagt
     7801 cgctccccgc gcaagttttg aggccgcgtt atccgcctga agtttttcgg ccgcgatcgc
     7861 ggaagtgccc gtggcaaggc ccaaaataac aaacgctaat aaaatttttt tcatttctat
     7921 cctccgggtt ttagatgatc agtacgccgt tcgttaaaat cgggtaatca ttgctcgtgt
     7981 cttgtttccc tcttttcaaa ggggaaccca acggggtcaa aaaagcccca cattcccccg
     8041 aaaggaaatt tctcccgaac ctggcaaaca ggacaacaaa ccggttaata aatcgtccat
     8101 ttataacaaa ttttgaggtt ttttatacca cgaattcttc agagtcaaag attgccgtcc
     8161 ccgccaaacc cttccgtctg aaaaacttcg atcttcgctc cagacagctc ggtctcatcg
     8221 agacccgcct tttcaagagc gcaactttca aaaaaagtct tcgcgtccca ccctgtttca
     8281 atcgcaacct ccggaagata gacccctttt ttccagctgc aactcacaat aatcccgtct
     8341 attccgacac gaatgtcttt ataggaacat atggacgccg gctccgtcag caaagagaca
     8401 tggatatgaa ggccgtccaa ttcactcagc gcgagcggag ggaacctcgg gtcttcaaac
     8461 gcggcggcca gcgccataca ccggatactt tcccggagcg gctcatggcc gatgatctga
     8521 ccaatgcaac ctcgcaacat ccctttgcgt tttaatgtca cgaaaattcc gcgttttttt
     8581 tccaggaatt cttcggagag gtccaactcg ggctccggcc catgagtgag tcgcgctttc
     8641 aataagtcat tgatgtaacg aagtgttttt tgttgttcgg attgacttag catcatctcg
     8701 gccactctct cttcgatcgg gccgcgaatc cggccccgtg ttcatgcctc cggattaact
     8761 ttgtacgcca tcgccgcgta accgacaacc gatccttttt ctcccgtaat atctcccgag
     8821 ttcgcgtact taagaagccg gggaccttcc caccccaatt tttccgccaa aaacaaggat
     8881 gtaatcacgg ggccaaagcc gcaagcttca atctcgcccg cgtagtttga acgttcaagt
     8941 ctcctcacat cacgatcccc tatcattcga atcagcttat ggtccatgtc caccgcttct
     9001 ttgtagggat gaaaatgtga caggtccgtg ctgaaaacaa aaagggcgcg ctttccggca
     9061 agctcgcggc tcaacgcttc cgaaagcgcc tccgcgttcg ccacggatgg ctcaccgata
     9121 taaacgggta agagggtaaa atccttgacc gccatctgaa gaaaagggag ctgaacctcc
     9181 agggaatgtt cattcagatc acccgagatg gtgtcggcca gataaggact ggcgtctctg
     9241 attttttcgg caagtgtttt gttcacgggg atccggccga gaggcgtttc ataaaaatcc
     9301 gctttatcaa taaagacgcc ttgaataaaa atccggtgta ggaaggccgc cacaacaatc
     9361 acctctgcct gggctccgtc aatcgcgcga taggcttccg cggcaattcc tccggagtac
     9421 atgtaccccg cgtgcggaac aataagcccc cgaacggaag gatcgggccg cgaactcgcg
     9481 tggtcgagaa aagtccgcac ctcgtgccgt aaaacatccg gccggtcagg ataaaactgt
     9541 cccgcgacaa ttcctttcct gatgatcggc ttttcgtgag acatggtctt tacctccccg
     9601 ggctattcta ccttcaaaaa accttcgcgc aaccttcctg tctgactata atgaagccat
     9661 gtatcaagtc aggatcgcgt cttttgacgc taaaccccat gtcaagattt cattcgaaaa
     9721 atgctaatcg cgcctgccaa tgtttctcac gctgttaaag ttgtcgaacg gtttaaaacg
     9781 cttttcatca tgatcaaata ttagtatcca taccctttta tgcttaatcg attttttaaa
     9841 ctccgggaac gacacaccac ccttggcacc gaggttgttg cgggaaccac gacattcctg
     9901 gccatggctt acattatcct tgtcaacccc gccatccttg cttcgacggg aatggataaa
     9961 acggccctga tccttgtcac ttgtcttgcc acggcgctgg cgacactcgc cacgggactt
    10021 tttgccaatg cccccatcgc gatggcgccg ggcatgggac tgaatgcgtt cttcgcctac
    10081 tccctggtcc tgacagacca cctcacctgg caaacggcgc ttggtgtcgt ctttttatcc
    10141 ggtctctttt tctttttact gactgccgtg gggatccgtc aaaaaatcgt cgaggcaatc
    10201 ccccgctccc tgatttcggc ggtttcggcc gggatcggcc ttttcattac gttcatcggt
    10261 ctggtcaacc tcgggatcat tcaaaaaaat gaggtgacgc ttgttgccgc ggcaccgatt
    10321 actccgaccg tcatggtcgg cctggcaggt tttttgctga tgcttctgct tgaaatgcac
    10381 cggatcaagg gggccatgat tttgggaatc atgatgtcaa ctctgatcgc gctttggaca
    10441 ggactggtgc cgtggccaaa ggagtttgtc acgtttcact ttgacctaag ccctatcgct
    10501 tttaaactgg atattctcgc cgcgctcaaa gggggcctct ttggcgcgat cttcagcctg
    10561 atgttcatgc atcttttcga ttcgatcggc tcccttgttg cctgttgcca ccaggcaaaa
    10621 agggtcgatg aaaaagggcg tttgatcggc cttgaccggc ttctctcgat cgacgcgctc
    10681 gcgagcatgg cgggcgccat ccttggcact tccacgatca ccgcttatgt cgagtctacc
    10741 accggaattg gccagggagg gagaacgggg cttacgtccg ttgtgaccgc tcttcttttt
    10801 gttctcgcga tcatctttgt cccactaatc tctgtcgtgc ctgtttacgc caccgccccc
    10861 gccctgatcc ttgtcggttt tttcatgatc aaggaagtgc gggaaattaa ttttgaggac
    10921 gtggaagaag gctttccggc atttgtgatt atcgtgatga tcgcgttaag ctattccata
    10981 gcgaccggcc ttgcattcgg gttcctcagc tatgtccttt tcaagatcct ccgcctgaag
    11041 ttcgcggagg tgaaaccggt catgtgggtc attgccggtt tgttatgtct tcattttata
    11101 gtttgagaat ccagtcaatg aaaaaccata ttctttcagt tcaaagtcgg tgaattaccc
    11161 tttccttggg ggcgggatac tttatgagtt ttttcgccag actgacccgc tttcttcagg
    11221 attctctgcc aaagaatttg gatttgttgg gccaagtcat ttttctcgac cttcttcccc
    11281 agccgtttga tccgccggtc gaattcctta ctgatggcgc tgtccgggac accgttaatg
    11341 atctcctgca aatcttcccg ccggatttcc cgaaggcgtg aaatcagctg ttcgcgagtc
    11401 atcgtctggg aaaacccgtt ttcgatcttg atccgcaccg gcagtttttc aattagtaac
    11461 cagtaagacc cgtagcggac cataaaatct tcgtcggaag ttttatcaag acgttcgata
    11521 taacgctgtt tctctttttt aaaatcgcag ctggcaagat aggcgttggc cagccctttg
    11581 aaggcgcttc cgatgatacg ctccctgacc gccggctcct cggcagaaac ggaaaaactc
    11641 agggaaagaa ttaaaacaaa acaacacaga aatcttttca cggaactcct tccttgtcgc
    11701 gtaacaccgc gatctatgaa ccgtcttaaa gcgttattat caaccgcgtt ttgaaataat
    11761 gcacggtttt tccattaggg aagtgttacc tcaaggcgta ttttattgtt ccatagcttt
    11821 cgatccggga ccataatacc ctcatgaaca gaactcctga cgccctctac tggaaagatc
    11881 ttcccgacgg acggatcgaa tgcgcgcttt gcccccgtcg ctgtaaactc ttcgatcagc
    11941 aacgcggatt ctgttttgtt cgggttcggg aaggaaacag gatggtcctc gccacttatg
    12001 gccgcacaag cggattttgc gttgacccga tcgagaaaaa acctcttttc cattttctgc
    12061 ccggatcttc catcctctcc ttcggtacgg caggatgtaa tctgggatgt ctcttttgtc
    12121 aaaactggaa cctctcaaag tcaaagggaa ccgacacggc cgccgaacag gcatcaccag
    12181 aggagatcgc aaaagcggca aaatcagccc ggtgcgaaag cgtcgctttc acttataacg
    12241 atcccgtcat ctttcacgaa tacgcggttg acacggccca ggcgtgccgg gaacacggca
    12301 tcaaaacagt cgcggtaacc gcagggtacg tttctcccga accgcgcagg gaattttaca
    12361 aatggatgga tgccgtcaac gtggacctga aaggatttac cgaaaatttt tacaaaaaat
    12421 attgccgggc gtcattaggc ccggtgcttg aaacgctcga atacatcaaa aaagaaacaa
    12481 aaacctggct cgagatcacg acactcctga tcccggggct caatgattcc gaaaaagaat
    12541 tgcatgacat gtcatcgtgg attgtcaaaa cgctggggcc cgatgtgccg atccatttca
    12601 ccgcattcca tccggactac aaaatgaacg ggatctctcc cacgccgccg gagacattac
    12661 aaaaagcgcg aacgatatcc cgagatcacg gcatccgtta ctgctacacc ggaaacgtca
    12721 gaaaccctga tggcgaatct accttttgcc atcattgcgg aaaaaaaata atcggccgga
    12781 actggtttaa acttactgag tggaacctaa aagatgggag ttgtcgattt tgcgaaaccc
    12841 gttgtccggg ccactttgaa gacgggccgg ggcgccaggg ggatcaggga aagtttatcc
    12901 ctttttaata atcggggaca aaaaactcgt cgtctctgtt gtgccaacca caaaaaaaca
    12961 acttaccggc ctcttcgccc gcgcaaacat gtcgtcccgc gatttcgtcg cttacaaatg
    13021 agttgatcgc tataggaatc ggccagcaaa tcttcgggag cgattcctaa caatttccgg
    13081 taatgatcac actgccgtcg caattccgtg atacggcatt ccggggttct tccctgcgcc
    13141 tcgatctcca caaatcttcc gagacccttc acttggtcga tatggaactt tacatttttt
    13201 ataaaataaa tttccctcat cttgtcgacg acagccaaaa ctccaaaggc cacattcagg
    13261 atttttttca tcaggtccgg ctcgggacac tgatataaaa acgcgtcgca tcgtttggat
    13321 tttttttgat tgctccgctg gtaaaaaatg agggcatttt cgatttgtcc ttcccgcagt
    13381 ttcaatcgcc ctcggggaac tctgaagtag gtatcgatct gatgatcggt cccgcggaaa
    13441 tcagctctcc gggacttaag aatttcccga atccgggaaa catcagaaca gcgggccttg
    13501 atttcaacga tgcgacactt catatctttc gatcaaacct ttccggcagg aatggaaaca
    13561 ataaaaatcg cttctttccc ggggctattt cccacgcgaa atttcccgtc attcttcccc
    13621 gccagaatcc cgcccagacc gcggaaaagg gaaaaaccaa aaaactcgcg cgattttttt
    13681 agttgccaga accaaagggg tttttaagtt ttccggcaat gcttttcacg gttccggcgg
    13741 cgtccttgac cgtatctttg gcgtctcccg cgagagatcc cgccatcgac gtcgtggtct
    13801 tgatggtgtc caaacccttc gccgcggttt gggaagcaag gtcggtcgct gaaccgacga
    13861 ttcctcccac gctcgactga aggcttccca gatcaaagtt cgtcaacatg gcaagaggtg
    13921 tctttgtcat cacgcgcagg acaatcaccc ggatcagggc cccctgttcg gtaatgtttt
    13981 catagctttc gtcgagatta atattgaatt cgcgaacgga ttcttgagct ccgctccgat
    14041 cgataaaaac cactttcccg atcttgagat ggacatgatc cagctggatc ggcatcgcct
    14101 ttgtttcttt tttgggtgtt tccggtttgc cggacgatgt cttgtcttgc tcgatccctt
    14161 taatcgaatt gatgttcaac tttccgtccc tgttcctcac caccgtaaat tggcgaagat
    14221 caaattcgac tttttccaaa tgaagtttac cgctcatgat atcggacaaa tgatagtcca
    14281 cgaacacctt cggcacatcc accatcgttt cgtccccgaa cccttcagga ttgccgacat
    14341 tcaacccctc aatatcaaga taagtttgct gaagaccaat atcaagcatc ttgatattga
    14401 ctggaagtcc aaccgcggcc ttcgcgccca actccacggc cgccttcacg attacattac
    14461 gggccagaac caccacgaca aaaacagcga cgataaaaat cagaatcttt ccgagaaatt
    14521 tcatgtgttt ctcctttttt gttgttgatg ttgcaaccct tgtcggggat gaaaccgacc
    14581 tttatttttt aagaaaagct ttttcctgaa attcaatcgc ccgcgcgatt tccgcatatc
    14641 ctcgcgactc agcccacttc cggagtgagg tgagaattgc cggatatttc ttctcgtcat
    14701 agccgtacat cagcatttcc tgataaatat cgcggaaaga agcttgaggg ttttgggaga
    14761 tcttgtaggc cgcgatcatc agccccgtcc gatcctttcc ggcccggcag tgcacaagcg
    14821 ccggctgttt cgcggtgtcc gaaataaccg aaagaaccgc gtcgattgtc ttgataggaa
    14881 cctcttcgcg cgcgctcatc gggaagtgat aatgctgaat ccccaaactt tccgttgtgt
    14941 ctctctccca gcgttcaatc gcgcgacctg agcgaaggtt cacaacggtt tttaacccgt
    15001 gggccttcat cctcaaaagt gattccttgc ttggctgggc cgagcgccaa acacccggcg
    15061 aaaccacatg gaagttgtgt tgtttcgcgc tgaccgaatg cggaggcgcg acataaagga
    15121 gtaccccgtc ggtcggcctc attttatgag aaaaagaaca aaaaacattt ttcccagcca
    15181 actggtgaac aacaaaccat ccggcagtga ctaacagaac gattccaagg atgactggaa
    15241 ttttaagagc tcgtctttta ttgtgattgc gtttgattgt catattttcc tattaaacgt
    15301 tgaatcgtcc tgcgcgcgct aaaaaaatca atccttcaat ggaagcttct tcgcgggaga
    15361 aaatccgatt tgagctattc tcattccatt caagcggaac gtcaagcttt tcgagaaaac
    15421 tcaggataag caccgatctc aaaaaccctc acaaactgtc cagccattcc accagacaaa
    15481 gaggaataca acaaaaagtg acgcaacaat ctgaacgtgc cccgatcatg ataatatccg
    15541 agagatccat aaagcgggag tcccgcatgg gattgagcat cgaaaagacc catgttccgc
    15601 tccacgaggc cacatcacac atcaggcagg gtttgttctt cgggagaatt ccgggcaaca
    15661 cagggcgata cgtctctgag gggtgaaata taaacattca cgtcaacaaa aaatatgacc
    15721 ggatttgaac ttgaaaagcc cctgcaaggc aacctggtta aggatttctt ggccggaata
    15781 acgtatcatc agttcatttt ttaaattgat cccgaagcgt tcgagctgtc tcaaccagat
    15841 taaaaatcgc ttgtcgggtt tcatcccaac cccgcgtttt taaaccacaa tccggattca
    15901 accaaaagtt ttcgggtggg attttttcta gaaaacgctt cgccagttcc tccatcttcg
    15961 ccaccgaagg gacttggggt gaatgaatgt cccaaactcc cagtccaatc tggcgtttaa
    16021 aatctgccct tccaaatgag cgaagaatat cttccccctt gtccctagaa acttctatcg
    16081 agatcacatc aaaatccatt cgcataatgt actgcataat ttctccaaag tgggagtaac
    16141 acatatgcgt atgaatctgg gtaataggat ctgtattgac cgtaagattg aacgatttta
    16201 ccgcccagtc aaaataatct ggccaatttc tttttttaac aggagccttc tctcgaaagg
    16261 cagcttcatc gacttggact attttgattc cattcgcttc atagtctcga atttcatcgc
    16321 gcaaacttaa ggcaatctga tacgcgactt cggaaactgg aatatcttca c
//
LOCUS       DAOSIO010000012        15853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_3871_length_15853_cov_5.097544, whole genome shotgun sequence.
ACCESSION   DAOSIO010000012 DAOSIO010000000
VERSION     DAOSIO010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 15853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15853
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3871_length_15853_cov_5.097544"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..508)
                     /locus_tag="PK590_01045"
     CDS             complement(170..508)
                     /locus_tag="PK590_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23215.1"
                     /translation="MKYRNRSKGFALVTVLVVVIVVAALSAGVIQVLNSYTSLKQVSL
                     EGIKAQYLAEAAMQWALYQCRSAGGVCPTTEDTIIQMPSGDLPDGMTSVISINAGSGG
                     TYDIKTTITY"
     gene            complement(505..1014)
                     /locus_tag="PK590_01050"
     CDS             complement(505..1014)
                     /locus_tag="PK590_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOG23216.1"
                     /translation="MELVRNQRGLGLLELIVAISLLAVVLMGATSIFLSMKKTGSSFY
                     ATESSLMEAVLAANEEMVQKITVSNSAVPDSTATPPNSKITIRVDEANTPANTSDDKT
                     YVYALNGSNLTRKIGTGAAVVIAKDIRALTFTPTGNNTITINLEVQPTGGGKEHIKTT
                     ATARGRSSR"
     gene            complement(1005..1454)
                     /locus_tag="PK590_01055"
     CDS             complement(1005..1454)
                     /locus_tag="PK590_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOG23217.1"
                     /translation="MKHQGLSLVEVVVSLVLLSVLALGVSSVFSLVSSPDRRSETGSR
                     DLQAANYARQTLEELKNAVSSDEMGAGAALLDGTNPGCKDDLDFVTEGSPCSEEGKTY
                     SKGTNPADPTSPVIAGTPFSRSYTVWDISDGKGGVACKKVTVNVTWN"
     gene            complement(1558..2766)
                     /locus_tag="PK590_01060"
     CDS             complement(1558..2766)
                     /locus_tag="PK590_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOG23218.1"
                     /translation="MKYKIITSFFCLSLIFPGFALAGQGLRESSFPCPANIQKIRVLV
                     VKPRNEIKISCVAPYEIHDSRGARVSAGEKLSVVSVRGQDPNILFGGRPVPGDLLIVQ
                     SAGAGVRVGDSRVYGDTIAIKRVAGNRVEVINYLPVDDYLKGVVPVEANPLWGLEALK
                     AQAVASRTFAVYKMTEQAKEPYDVTSGVYSQVYAGKKIENPRTNQAVEDTAGEVLTFK
                     KRIFPGYFHSTCGGRTTDASQVWRIKPLEPLRGVECKFCAKSPHYRWESVFTPEEIKT
                     KMAEKGGIPVQEVKGIRCGKIDLSGRAHEMIIRSTWMEKTVDADAFRVWMGPDKFKSN
                     LITKISTTPEGKFKIRGRGWGHGAGMCQYGMKYFGELGYGYREILGYYYPGAEIVRLP
                     DSGRGNVFEK"
     gene            complement(3080..3574)
                     /locus_tag="PK590_01065"
     CDS             complement(3080..3574)
                     /locus_tag="PK590_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HOG23219.1"
                     /translation="MGKTTRLTFLFVLIGLVALSGCASRKTSKKVDTLQAQMGAVTDE
                     LVRLDQSLQDTRAAISAEEAYQRQLQSAIVESESRLGALQEEETVITNIYRTPSGFEL
                     PAVNIQKALKKAGYYQGALDGKIGPATRDALKAFQRDNGLTADGVCGRQTWSKLKVYL
                     DSIK"
     gene            complement(3761..4567)
                     /locus_tag="PK590_01070"
     CDS             complement(3761..4567)
                     /locus_tag="PK590_01070"
                     /inference="COORDINATES: protein motif:HMM:NF020287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HOG23220.1"
                     /translation="MENSTPKKNLTIKEGDAVLLCFEDEKSYLIEVTRGKRASIHCGR
                     PILVDDWIGQPFGKKVVCEHGSGYLLKPTLEDLMMKASRESGIIYPKDAAFLIMKAGI
                     TNGSRVIEVGTGSGSLTMALAQTVAPNGHVYTYDRRTDLPKNAHKNVARAGLNDFVTF
                     KQRQASDPFDETGVDAIILDIPQPWEEVASVKRALRGGGRVVSLNPTFNQIEKMAEEL
                     RANGFILVESRELLEREILARGGKTRPVQRMIGHTEFMLFAVCPLDPTQP"
     gene            complement(4615..5265)
                     /locus_tag="PK590_01075"
     CDS             complement(4615..5265)
                     /locus_tag="PK590_01075"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOG23221.1"
                     /translation="MKKLIIYDLDGTLVDTRQDIINSVRYALKELNGPPVTDDEIKDA
                     VGTGLHSLIRQVFRTDDEKLADRGAQLYRKHYTSHMLDHSRLYPGARELLEFFKPRTQ
                     AVITNKPNPFSKQILEALGVGGYFIDVLAGDNGLPFKPDPASLFYLMGETKAKPEETL
                     FIGDSPVDIQTARNAGVEIVCLGHGFTPEKTLARANPDYLVHHYDEILKLARQKKW"
     gene            complement(5262..6056)
                     /locus_tag="PK590_01080"
     CDS             complement(5262..6056)
                     /locus_tag="PK590_01080"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG23222.1"
                     /translation="MGDFAGHALGPDSRVLAFKKLTGSLRGLSKGIRSVSRGDLKYKI
                     DVRGNDEIAELSRDFNRMAEDLNRTHQKNQAYFYGVIRSLVTILEARDPYTRGHSERV
                     AELSANMATQMGLAPDRIEMLKQTAILHDIGKLGIHEAILCKKEKLTERDWELLRTHP
                     ALGEDILKPVSLDPEILAAVRGHHERHDGTGYPDRIAGEDIHVFSRILSVADAYDAMT
                     TTRSYRQAMKHDEAIAELKKHSGSQFDPKIIDLLIRCLTQGEKPAS"
     gene            complement(5993..6670)
                     /locus_tag="PK590_01085"
     CDS             complement(5993..6670)
                     /locus_tag="PK590_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23223.1"
                     /translation="MNRLKRWLESFRVRVTLTLVFSMLFMGVVSDTMLQHAALQVQFE
                     NLRDRLKAAASIVASAIDAEMIRQIPLNRSGVQSPMFRALFERFEKIKQENPWVGFIY
                     ILAPKEGGARWQFVVDADPYLLHRKNVTAYPGDEYDVTNFPEIREALKGPVAERIIKS
                     DDWGTMLSGYAPIVDGDGNPVAIVGVDTLVSDIYRMRKKIQEKVLWAILLGTLWALIL
                     GFWLSKN"
     gene            complement(6667..10020)
                     /locus_tag="PK590_01090"
     CDS             complement(6667..10020)
                     /locus_tag="PK590_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOG23224.1"
                     /translation="MNRQKPASLSLTQNQKQAIRELDTNILVSAGAGTGKTRVLVERI
                     LHILKNNKAGISELLVLTFTDKAANEIKVRLSERLRDMGLERARRELEQVAIGTFHGF
                     ATRLLKEHPVESGVDPDFRVIENERADLLKDEIVTTVLETSFQESDEAFQLVRYLGET
                     AVRSAILKLFATARHEGKMFSVFFEENDLKRNALLERAEKEILRECERLIPKLSGELD
                     ETAWSRFVKNQRWDWNTLSDFREWKGSYSLKRKEGWKEWRVLADELAALRMEPLAVPW
                     RAHLENLALRFETAYEFQKQEENGLDFDDLQIRAVNLFLDPRPAMRKIRDRYQQRFKF
                     ILVDEFQDTNYLQTRFVDLLSSGSNVFVVGDFKQSIYGFRGAEPGIFLARETQSREDP
                     GWIRVPLTENFRSRAALLKTLNEIFRELWDEDQFPFEALSARAEPDPRHEGQVPVEIL
                     VTHLDPGEEKAHARIREALAIAGRLKDLHEKEGVPYGDMALLFRGMTMSGIYEYAMKV
                     FDIPYFIVAGRGFYAQPEIRDLANFLTHLEKPLLDIPLAASLRSPLFHVTDSTLFWLS
                     RWAKTGDERNPLAEALKEKRYEKIPEISQEQKKSLAEFSALADELRGLKDRISLAELI
                     DKILERTGYELSILATPGGARRYANVKKLMAMVREHESFDRMPLARFLAMMERLEVQE
                     VRESEAQISAEGEESVRMMTIHGAKGLEFPVVVVADLGARGGGQESKAVVAHANEGYA
                     LRLVNEVTRAAEEPFFYRKLDEKITAREREERKRLFYVACTRARSRLFLSGVHEVRKN
                     PKNSYGDMLTWMDWVMTIGEKLGIPKASGHDRTPNFVKKNADNFREQMERTLKELVPV
                     EGEPLPEKTGLVTTILKSVDLPVSAYVLFVKDPLAFWRAYQIGWKTTGGDVEGRVPSG
                     TDDEESVALDFPLSPDSRDFGTAMHAFLECFDIRHPEKYLEEEALDRFFKGLGGEAVP
                     KARNILKKFFSAPLFRKLQAARKVEREIDFVLNERRGLIHGKLDMLFQDAEGAWHVLD
                     FKTAEGDAVAARTSGYDLQIELYALAVHRILGFTVRSGIIYYLENSSAVTLDFSGQGD
                     GLEKTREKITGLQEAILDFCNQKMGAGKV"
     gene            10121..10555
                     /locus_tag="PK590_01095"
     CDS             10121..10555
                     /locus_tag="PK590_01095"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOG23225.1"
                     /translation="MTTDQRKFYRHPIKAPIRLREVTGNDARDSKSEDISSGGLSFFW
                     PSFLAKGTRLELSIPIEQQLFKMKARVAYCRKDGGMDLFRTGVCFEDSTSAFRAKLAE
                     EIIQIRNYREQMRSLRGKEISEEEAADQWIRRNAENFAKLLG"
     gene            complement(10639..11592)
                     /locus_tag="PK590_01100"
     CDS             complement(10639..11592)
                     /locus_tag="PK590_01100"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOG23226.1"
                     /translation="MMGRGSRVAQVGRHLEMLRRYFSFKKFFNLCRAFFCFLVGREKI
                     PTMPAFLKAEVSRKCEMGCPLCWAYAKKEERFYPLDKFKRLIDLFSPWIFEVSLYDIG
                     EPLWCENLNDYIRYAHQKRVGTSISTSLSLVKEDSYWEDLVRSGLDCLIVAIDGISED
                     VYKKYRRNGDLNLVMANLEKILAARKKYRAKLVIEWQMIDFEWNRHEQDAACRMSRKM
                     GIDIFRLIQNAIPPRFEAQKDREFLRKRNCLLPYIIFIVNVFNEVNPCYKYYNEHMKI
                     GDLEKESFAEIWNGENIAQIRSPRLIRQRKICNKCFEASLF"
     gene            complement(11711..12463)
                     /locus_tag="PK590_01105"
     CDS             complement(11711..12463)
                     /locus_tag="PK590_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOG23227.1"
                     /translation="MKFKFNLFGKKKKISSGIYSVYQPIVDIATWDIKGYEALVRSEK
                     ETPLEMFQKSYEDGTVVPFDFHCLEVAIGALPNLAEGQYLFLNIESPTLEQAFDKGKK
                     GEVFLDGVKEYSEKIVFELAEGVKRSDFEFIRRGVTFIRSLGYRFALDDLTEIGAKTF
                     QMASLRPDFFKIDLRLVRGIADNHVNQQIVKEMVHLGRMNGSEMVAEGVENKEDYKYI
                     RNMGIRYGQGFYFARPEKELLTYLEPPEEART"
     gene            complement(12493..13683)
                     /locus_tag="PK590_01110"
     CDS             complement(12493..13683)
                     /locus_tag="PK590_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HOG23228.1"
                     /translation="MKEREQSQKISWPHFEKDEIAAVSRVLKSGKVNYWTGSEGRLFE
                     KEFALFVGCRYAVALANGTVALETALRALGIGPGDDVIVPSRTFIASASCAVMCGAWP
                     IVADVDSVSQNITVDTLKAVLTSRTRAIIAVHLAGWPCDMKPIMAFARQRGLKVIEDC
                     AQALGASYQGRPVGSLGHVAAFSFCQDKILTTGGEGGMLTTNDQAIWKKAWTYKDHGK
                     RHGTSSGLKFLPRFQWVHDSFGTNGRMTEMQAAIGRVQLRKVRRWVRKRRTHAEILTS
                     YFKKIPAFRITMPTIETGHSFYKYYVFLRPEKLKKGWSRARILRAMNRKGIFCSVGSC
                     GEIYLEKAFRKKNLGPVRRFPVARKLAETSLMFLIHPTLSDRHLRRVGKVVTEIMSRA
                     SAPA"
     gene            complement(13693..14100)
                     /locus_tag="PK590_01115"
     CDS             complement(13693..14100)
                     /locus_tag="PK590_01115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOG23229.1"
                     /translation="MKQNNEKRFAKWKRPRIVDGRPTRYGWIVQGKKNFKLGKNTDIG
                     AFTYIHAKHGVFIEDEVQVGSHCSIYSHSTIDGKKGSVTLGRNCKIGSHSVMMPGVRV
                     GKNSVVGAFSFVNKSIPDNVVAWGVPARVMRKV"
     gene            complement(14097..14744)
                     /locus_tag="PK590_01120"
     CDS             complement(14097..14744)
                     /locus_tag="PK590_01120"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOG23230.1"
                     /translation="MRNRPLLKIKRAIDFCLAFFCLVAGAPVLGLCVACLWIAQGRPI
                     FFKQVRPGYRGRPFVLYKFRTMHETKNNHGDLLPDGQRLSFVGRFFRSMSLDELPEIW
                     NVLKGDMSFIGPRPLLMQYLSRYTSEQMRRHDMMPGITGWAQVHGRNAISWEEKFKYD
                     VWYVDHWSFWLDLKIFFLTIWKVFKREGISQAGQATAQEFKGSSDKAVTGKDLNS"
     gene            complement(14767..>15853)
                     /locus_tag="PK590_01125"
     CDS             complement(14767..>15853)
                     /locus_tag="PK590_01125"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOG23231.1"
                     /translation="SSVAREIDVQDLLGRNPVVPNIAFLEKNLNGKRFLVTGGCGSIG
                     KELVKQIARYGPGKIIVVDVNETGIFWLDYELSRLLSRDRYVLYLCDVRNIGKLEKIV
                     DRERPEVIYHAAAYKHVPISAQNPSEYVSTNVLGTKNVIDLAQRKDFIKKFCLVSTDK
                     AVNPVNVMGVTKRMAELLVLRETPNREDKKITFVRFGNVLGSRGSVVPLFKDMIEKGG
                     PVKVTHPDVERYFMDLQEAVHLVLQAPFFTGNGAAFILDMGTPVKIQDLARLAIRLSG
                     LEKEGIRIEYTGLRPGEKITEELYDPRYERWDKTDFEKIAALTAIRVVPEISDKQFEK
                     VNSFALAGDDESVYRWLKEFFPQISVF"
BASE COUNT         3837 a         4506 c         3563 g         3947 t
ORIGIN      
        1 gtgattgcct gcctggcgtt ttccttgtcg atggttctta aaaaattcgg aactgaaatc
       61 gcgacaagaa cactcgccac gaccacaaca atgagaattt ccgccaaaga aaaacctttt
      121 ttactcattt tatttttcct cacattcagt tttcacgaaa gcctggtatt taataagtaa
      181 tggttgtctt gatgtcatag gttccgccag aaccagcgtt aatagaaata acactcgtca
      241 tcccgtctgg caaatcaccc gatggcatct ggataatagt atcctcagta gtgggacaaa
      301 caccgcccgc cgatctgcac tgatagagcg cccactgcat cgcggcctca gccaggtact
      361 gagccttgat cccttccaga ctcacctgtt tcagactggt ataactgtta agcacctgaa
      421 tcactccggc cgaaagcgcc gcgacaacaa tcacgacgac aagcacggta accaaggcaa
      481 accccttgct tcggttcctg tatttcatcg agaactcctc cctcgcgcag tagcggtggt
      541 tttgatatgc tccttccctc cccccgtcgg ctgaacctcc aggttgatag tgatggtgtt
      601 attcccggtc ggtgtaaatg tcagtgccct aatatctttg gcaattacaa ccgccgcccc
      661 cgtgccaatt tttcgagtga gattactccc atttaacgcg tacacatagg ttttatcatc
      721 cgaggtattg gcgggagtat tggcttcatc gaccctgatt gtgatttttg agttcggggg
      781 agttgccgta gaatcaggta ccgcactgtt ggaaaccgta atcttctgga ccatttcctc
      841 attcgccgcc agcaccgctt ccatgaggga agattcggtc gcgtaaaaac tgcttcctgt
      901 ttttttcatc gaaagaaaaa tggaagtagc tcccatcaaa acaaccgcga gaagagagat
      961 cgccacaatt aattccaaaa gcccgagccc ccgctggttc cggactaatt ccatgttaca
     1021 ttcaccgtca ccttcttgca ggcaacgcct cccttcccat cggaaatatc ccagacggtg
     1081 taacttcggg aaaacggagt acccgcgatc acggggcttg tcggatcggc aggattggtc
     1141 cccttagaat aggtttttcc ctcttcactg cagggagagc cttcagtgac aaaatctaag
     1201 tcatccttac aacccggatt ggtaccatca agaagagcgg cgccggcgcc catttcatcg
     1261 gagcttacgg cattcttgag ctcctcgaga gtttgacggg cgtaatttgc ggcttgaaga
     1321 tcgcgacttc cggtttcgct acggcggtcg ggactactaa cgagggaaaa cacggaagaa
     1381 acgcccaatg cgaggaccga gaggagaacc agactgacca ccacctcaac cagcgacaac
     1441 ccttgatgtt tcacagtagc cccctctttt tttaaagttc tatcgcggaa cgtcactatg
     1501 ctacttttta aaaaaaacaa gtcaatcttt ctttgaagcg atcaagccac agcccgttta
     1561 cttttcgaag acattgccac ggccggaatc gggaagtctg acaatctcgg ctccaggata
     1621 ataataaccg agaatttccc ggtatccgta acccagttcg ccgaaatact tcatcccgta
     1681 ctggcacatc ccggccccat gcccccatcc acggcccctg attttgaatt ttccttccgg
     1741 ggtcgtggaa attttagtga tcaggttgct tttgaatttg tcggggccca tccagacccg
     1801 gaacgcgtcc gcgtccaccg ttttttccat ccacgtcgag cgaatgatca tctcatgcgc
     1861 ccgccccgac aaatcaattt ttccgcacct tatgcctttc acttcctgga ccgggatccc
     1921 gcccttttcg gccatctttg tcttgatttc ctcaggggta aaaaccgatt cccaacggta
     1981 atgtggcgac ttcgcgcaaa acttgcactc gactcccctg agcggttcca ggggctttat
     2041 tctccaaacc tgactggcat ccgtggtacg cccgccacac gtggaatgaa aatatcccgg
     2101 aaaaattctt tttttaaagg tcagaacctc gcccgccgtg tcctcgaccg cttgatttgt
     2161 tctgggattt tcaatttttt taccggcata aacctggctg taaacgcccg aagtaacatc
     2221 gtaaggttct ttcgcctgct ccgtcatttt ataaacggcg aaagttcggg acgcgacagc
     2281 ctgcgccttc agggcctcaa gcccccaaag aggattagct tcaaccggca cgacaccttt
     2341 caaataatca tccacgggaa gatagttaat cacctcgacc cggtttcccg ccactctctt
     2401 gatcgcgatc gtgtcaccgt aaacccgtga atcccccacg cgaacgcccg ccccggcgct
     2461 ttgaacgata agcaaatctc cggggaccgg tctgccccca aaaagaatgt ttgggtcctg
     2521 tccccgcacg ctcacgaccg aaagtttttc tccggccgat actcgcgcgc cccgggaatc
     2581 gtggatttca tagggcgcca cgcaggaaat tttgatttca ttacgcggct tgactaccag
     2641 cacccgaatt ttttgaatgt tcgcgggaca aggaaaggaa ctttcccgaa gaccctgccc
     2701 ggctagggca aatcccggaa aaataagact taaacaaaaa aaactggtga ttattttata
     2761 tttcatgact ttcccgtttc ctgacctgat ttctacgttt ttaagtcaaa accattatcc
     2821 cttgttacgg ttcaaagggt cgtcgcattg actgcgcgaa agcgttttcc ccgatgccgt
     2881 tatcctgccc acgacctaga gggagcgaag cgaaggattt tgcttcagat tctcttttcg
     2941 ggtccaaacg ccatcaaggt cgccagcgga acgcggcgga aaaaccaaaa aatcgaaaaa
     3001 tgcaaacttc caaataaaaa cccccgcaac ctgttttcag gatgcggggg tttctcaaaa
     3061 aaaccttcgc gtgacgctct tacttgatgg aatcgagata aactttgagt ttggaccagg
     3121 tctgtcggcc gcaaacacca tccgcggtaa gtccgttatc ccgttgaaag gccttgagcg
     3181 cgtcgcgcgt cgccggcccg atttttccgt caagggcccc ttgataatac ccggccttct
     3241 tgagcgcttt ctgaatgttg acggccggaa gttcaaaccc tgaaggcgtc cggtagatat
     3301 tggtaatgac cgtctcttct tcctgcaaag ctccgagccg actttccgac tcaacgatcg
     3361 cggactgcaa ttgtctctgg tacgcctctt ccgcggaaat cgccgcgcgc gtatcttgca
     3421 acgactgatc caacctgacc agctcatcgg taaccgcgcc catctgcgcc tgaagcgtgt
     3481 cgaccttttt ggaggtcttc cgcgacgcgc aaccactgag cgccacaagc ccgatcaaaa
     3541 caaaaagaaa cgtcaaacga gttgtttttc ccatcgtgat ttctcctgat tggtgatctt
     3601 ctaagttaaa taatggctac gagagcccca agtctaccaa aatcaattcg gcttagcaat
     3661 cccaattcaa taaatcagaa aacgaggctt tgggtttcgg ccgtttgata ttgcgcacca
     3721 agaatccgcc ggcctttttt gtctttcccc aaaaaatacg tcagggctgg gttgggtcaa
     3781 gcgggcaaac cgcgaagagc ataaattccg tgtgaccgat catgcgctgg acgggacggg
     3841 ttttgccgcc cctcgcgagg atttccctct cgagaagctc ccggctttcc acaaggataa
     3901 atccgttcgc tctcaactcc tcagccattt tttcgatctg gttaaaggtc gggttcaaac
     3961 tgacgacccg gccgccgccg cgcaatgcgc gcttcacgga agccacttct tcccagggct
     4021 ggggaatatc aagaataatg gcgtccactc cggtctcatc aaaaggatcc gacgcctgcc
     4081 gctgtttgaa agtgacaaaa tcattgagac ccgcgcgcgc cacgttttta tgcgcgttct
     4141 tcggaaggtc cgtccggcgg tcgtaagtgt agacgtgccc gttcggcgcc accgtttggg
     4201 cgagtgccat cgtcagagac cccgagcccg tgcccacctc gatcacccgc gaaccgttcg
     4261 tgataccggc tttcatgatg aggaacgccg cgtctttcgg gtagataatc ccgctttcgc
     4321 gtgacgcctt catcatgagg tcttccaggg tgggtttcag gagatatccg gagccgtgtt
     4381 cacaaacaac ttttttaccg aagggctggc caatccagtc atcaaccaga atcggacgac
     4441 cgcaatggat cgacgcgcgc ttgccgcgtg tcacttcaat caggtagctt ttttcgtctt
     4501 caaagcagag gagaaccgcg tcgccttcct tgatcgtcag attttttttc ggggttgagt
     4561 tttccacggg cttaaactat cagatattct gaatttctca atttataaaa aactctacca
     4621 ttttttttgc cgggcaagct tcaggatttc gtcgtaatgg tggaccaggt agtcggggtt
     4681 cgcacgcgcc agagtttttt ccggcgtaaa accgtggccg agacaaacaa tttcaactcc
     4741 ggcatttcga gcggtctgga tgtcaaccgg actgtccccg ataaaaaggg tttcttccgg
     4801 tttcgctttg gtttcgccca tcaggtaaaa aagagacgca gggtccggtt tgaacggaag
     4861 cccgttatcc cccgccaaaa cgtcgatgaa ataaccgccc acgcccaggg cctcaaggat
     4921 ttgttttgaa aaaggattgg gcttgtttgt aatcaccgcc tgggttcgcg gtttgaaaaa
     4981 ttcgagcagt tccctggctc ccggataaag acgcgaatgg tccagcatgt ggctcgtgta
     5041 atgcttccgg tagagttggg ccccgcgatc ggccagtttc tcgtcgtccg tccggaaaac
     5101 ctggcgaatc agcgaatgca atcccgttcc gacggcatcc ttgatttcgt catcagtcac
     5161 cgggggaccg tttaactctt taagcgcgta gcggacgcta ttgatgatgt cctgccttgt
     5221 atccaccagc gtcccgtcga gatcgtaaat aatgagtttt ttcatgaggc gggtttttct
     5281 ccctgcgtca aacaacggat caggagatcg attatcttcg ggtcaaactg ggatccactg
     5341 tgttttttta gttcggcaat cgcttcgtcg tgtttcatgg cctgacggta agaccttgtg
     5401 gtcgtcatcg cgtcgtaggc atccgcgact gaaagaattc tggagaaaac atgaatgtct
     5461 tctcccgcga tccggtcagg ataccccgtc ccgtcatgcc gctcatgatg tccccgaacc
     5521 gccgccaata tctccggatc cagcgacaca ggtttcaaaa tatcttcgcc aagagcgggg
     5581 tgtgttcgaa gcagttccca atccctctcg gtcagttttt ccttcttaca aaggatggct
     5641 tcatgaatcc cgagtttgcc gatgtcgtgg agaatggccg tctgcttcag catttcgatc
     5701 cggtcgggag caagtcccat ctgtgttgcc atgttagccg aaagctcggc gacccgctcc
     5761 gagtgcccac gggtgtaagg gtctctcgct tcaaggattg tgaccagcga ccggatgacc
     5821 ccgtaaaaat aagcctggtt tttctggtgg gtacgattca gatcctcggc catccggtta
     5881 aaatcccggg acaattcggc gatctcgtcg tttcccctga cgtcgatttt atatttcaga
     5941 tcgccgcggc tcacgctccg gattcccttt gaaagtcccc gcaatgaccc ggtcagtttt
     6001 ttgaaagcca gaacccgaga atcagggccc agagcgtgcc cagcaaaatc gcccacaaaa
     6061 ctttttcctg aatttttttc cgcatccggt aaatgtccga gacaagcgta tccactccca
     6121 cgatcgcgac cggattcccg tccccgtcaa caatgggcgc gtaacccgag agcatggttc
     6181 cccagtcgtc acttttgata atccgttccg ctaccggacc tttaagcgcc tcacggatct
     6241 ccggaaaatt cgtgacgtca tattcatcgc ccggataagc ggtcacgttt tttctatgta
     6301 agagataagg atcggcatcc accacaaact gccagcgggc acccccttct ttcggcgcca
     6361 gaatgtaaat gaaacccacc cagggatttt cctgtttgat tttttcaaat cgttcaaaaa
     6421 gcgcgcgaaa catcggggat tgaactccgc tccggtttaa cggaatctga cggatcatct
     6481 cggcatcgat cgcggacgct acgatactgg ccgccgcctt aagacgatcc cgaagatttt
     6541 caaactggac ttgaagagcc gcgtgctgaa gcattgtatc gcttactact cccataaaaa
     6601 gcatcgaaaa aaccagggtc agtgtcacgc gaacccgaaa actttccagc catcgtttca
     6661 atcgattcat acctttccgg ctcccatctt ttgattgcaa aagtccaaaa tcgcttcctg
     6721 taatccggtg atcttctccc tggtcttttc cagaccgtca ccctggcctg aaaaatccag
     6781 cgtcaccgcg gatgaatttt cgagataata aatgattccg gaacgaacgg taaagccaag
     6841 aatgcggtga accgccaggg catatagttc aatctgaaga tcatacccgc ttgtccgggc
     6901 cgcgaccgcg tccccttccg ccgtcttgaa atccaggaca tgccacgccc cttcggcgtc
     6961 ctgaaagagc atgtccagtt taccatgaat aagtccccgg cgttcattca gcacaaaatc
     7021 tatttcccgc tccactttcc tggcggcctg gagtttccgg aaaagaggcg cactgaaaaa
     7081 ttttttcaaa atgttcctgg ccttggggac agcctcccct cccaatcctt taaaaaaacg
     7141 gtccagcgct tcctcctcca gatatttctc gggatgacgg atatcaaaac attccagaaa
     7201 cgcatgcatc gccgttccaa aatcccggga atccggtgac aggggaaaat ccaaagctac
     7261 ggattcttca tcgtccgtgc cactgggaac ccgtccttca acgtctcccc cggttgtttt
     7321 ccagcctatc tggtaagctc tccagaacgc cagcggatct ttcacaaaca ggacgtaagc
     7381 ggaaacggga agatcaacgc ttttaaggat cgtcgtgacc agcccggttt tttccggcaa
     7441 aggttccccc tccaccggca ccagttcctt cagagttcgt tccatttgtt cccgaaagtt
     7501 gtccgcattt tttttcacaa aatttggcgt acgatcatga ccgcttgcct tcggaatccc
     7561 gagcttctca ccaatcgtca tgacccagtc catccaggtc aacatatccc cgtagctgtt
     7621 ttttgggttt ttcctgacct cgtgaacccc gctcaaaaaa agccgtgatc tggcgcgcgt
     7681 gcaggcgaca taaaacaacc gtttgcgttc ttcccgttca cgggccgtga ttttttcatc
     7741 cagtttccga taaaagaaag gttcttccgc cgcgcgcgtc acctcattca ccaaccgaag
     7801 agcgtaaccc tcgttggcgt gcgccacgac cgccttggac tcttgccctc ctccccgcgc
     7861 tcccagatcc gccaccacca cgacgggaaa ttcaagcccc ttcgcgccgt gaatcgtcat
     7921 cattcgaaca gactcttcgc cttcagcgga aatctgcgct tcggattcac ggacttcctg
     7981 aacttcaagt cgttccatca tggctagaaa cctggccagc ggcatccggt cgaacgattc
     8041 atgctcccga accatcgcca tgagtttttt gacattggcg tagcggcgcg cccccccggg
     8101 cgtcgcgaga atagaaagct catatcccgt ccgttccaaa atcttgtcga tgagttccgc
     8161 gagagagatt cggtccttca ggccccgcag ttcatcggca agggctgaaa attcggccag
     8221 ggactttttt tgctcctgtg aaatttccgg aatcttttca tatctttttt ctttcaacgc
     8281 ttcggccagc ggatttcgtt catcccctgt cttggcccat cttgaaagcc agaacagcgt
     8341 gctgtccgta acgtgaaaaa gcggagagcg cagagacgcc gcgagaggga tatccagaag
     8401 cggtttttca agatgcgtca gaaaattcgc cagatctcga atctcgggct gggcgtagaa
     8461 accgcgacct gccacaataa aatatggaat gtcgaaaacc ttcatcgcgt actcgtaaat
     8521 cccgctcatc gtcataccgc gaaaaagaag ggccatgtct ccgtaaggga cgccttcctt
     8581 ttcatgcaga tccttcagcc ggccggcgat cgccagagcc tcacggatcc tcgcgtgcgc
     8641 tttttcctca cccggatcaa ggtgggtcac aaggatttca acggggactt gcccttcgtg
     8701 cctgggatcc ggttcggccc gagcggaaag cgcctcaaag ggaaactggt cttcgtccca
     8761 aagttctcta aaaatctcgt tcagggtctt gaggagagcg gcgcggcttc tgaagttttc
     8821 cgtgagggga acccggatcc atccgggatc ttcgcgggat tgcgtttctc tcgcgagaaa
     8881 gatgccgggt tcggcgccgc gaaaaccgta aatcgactgc ttgaaatccc cgaccacgaa
     8941 cacattcgac ccggacgaaa gcagatcgac aaatcgcgtc tggagataat tggtatcctg
     9001 aaattcgtcg accaggataa acttgaacct ttgctggtaa cggtctctga ttttccgcat
     9061 ggccgggcgc ggatccagga aaaggttcac ggcccgaatt tgcagatcgt caaaatccag
     9121 cccgttctct tcctgttttt ggaactcgta ggccgtttca aaacggagcg caagattttc
     9181 cagatgggcg cgccagggaa ccgccagagg ttccattctg agggccgcca gttcgtcggc
     9241 gaggacacgc cactccttcc acccctcctt gcgcttcaat gaatacgatc ccttccattc
     9301 cctgaagtcc gagagtgtgt tccaatccca ccgttgattt ttcacaaagc ggctccacgc
     9361 agtttcgtcc aactctccgg aaagtttcgg gatcaatcgt tcgcattcac gaaggatttc
     9421 tttctccgcc ctctccaaaa gggcgtttcg tttcaaatca ttttcttcaa aaaacaccga
     9481 aaacattttt ccttcgtgcc gtgccgtagc gaacaatttc aggatggcgg accggacagc
     9541 ggtctctccc aggtaacgca cgagttgaaa cgcttcatcc gactcctgaa aactggtttc
     9601 cagtaccgtc gtcacaattt catccttaag gagatcggcg cgctcgtttt cgatcactcg
     9661 aaaatccggg tcaacgcctg attcgaccgg atgctctttc aggagccggg tcgcaaatcc
     9721 gtgaaatgtt ccgatagcga cctgctccag ttcccggcga gctcgctcaa gccccatgtc
     9781 ccgaagtctc tcggaaagtc ttactttaat ctcattggcc gccttgtcgg tgaaagttaa
     9841 aacgagaagt tcggaaattc ccgctttgtt atttttcagg atatggagga ttcgttcgac
     9901 tagaacacgg gttttccctg tcccggctcc cgcggacacg agaatattgg tgtcaagctc
     9961 gcggatagcc tgcttctgat tttgggtaag agataaactt gctggttttt gacgattcat
    10021 gagcgtgaag ttttgatttt gaagccgttc agggaagcgg cattatacaa aatcttttcc
    10081 ggctatggaa aaaaaccaaa catcagctat actttgatcc atgacgaccg atcagagaaa
    10141 attttacaga cacccgatta aggcgcccat ccggctgagg gaagtcacgg ggaacgatgc
    10201 gcgcgattct aaaagcgagg atatttcgag cgggggcctt tccttctttt ggccttcttt
    10261 cctggcgaaa ggaacgcgcc ttgaactctc gatcccgatt gaacaacagc ttttcaaaat
    10321 gaaagcgcgc gttgcctact gccgaaagga tggtggcatg gacctcttca gaaccggagt
    10381 ctgttttgag gattccacga gcgcgtttcg cgcgaaactc gccgaggaaa tcattcaaat
    10441 tcgtaactac cgtgagcaaa tgcgatcgct ccgcgggaaa gagatcagtg aagaagaagc
    10501 cgcggaccag tggatccgcc ggaacgccga aaacttcgcg aaacttctgg gttaatccgc
    10561 gcttccgctc gcaataccgc acacaagctt cctcctcgcg cgcctcgacc ttttccgtat
    10621 ccggaagata accggctttc aaaagagcga tgcttcaaaa catttattgc agatcttcct
    10681 ctgcctaata agccggggac ttcgaatctg cgcgatattt tccccgttcc agatctccgc
    10741 gaaactctct ttttctagat cgccgatctt catgtgctcg ttgtagtact tatagcaggg
    10801 attaacctcg ttaaagacat taacgataaa aatgatgtag ggcaaaagac agtttctttt
    10861 tctcaaaaat tcccgatcct tctgggcctc gaaacgggga gggatcgcgt tttgaatgag
    10921 cctgaaaata tcaatcccca ttttccgtga cattcggcaa gcggcatctt gctcatgccg
    10981 gttccattca aaatcaatca tctgccattc aataacgagc ttggcgcggt actttttccg
    11041 agccgccaga attttctcca aattagccat caccaaattc agatcgccgt ttctccggta
    11101 ttttttgtac acatcttcgc tgatcccatc aatcgctacg atcaaacaat ccagacccga
    11161 ccgcacaaga tcttcccagt aagagtcctc cttaaccaga gacaggctcg tagaaatcga
    11221 tgtcccgaca cgtttttggt gagcataccg gatatagtcg ttaaggtttt cacaccacaa
    11281 gggctcacca atatcgtaaa gtgaaacttc aaaaatccat ggggaaaaca aatcaataag
    11341 ccgtttgaat ttgtccagcg ggtaaaaacg ctcctctttc ttcgcgtaag cccagcacaa
    11401 ggggcatccc atctcgcact tgcggctgac ttctgctttt aagaatgcgg gcatcgtcgg
    11461 aattttttcc cgacccacaa gaaaacaaaa aaaggcgcga cataaattga aaaacttctt
    11521 aaatgaaaag tagcgtcgaa gcatttcaag atgcctgccc acttgcgcta ccctggaacc
    11581 tcgccccatc atgctaatcg ctccctcccg ctaaagagct ttcgtgaaaa acaagcgttg
    11641 gcacgctcac cgcgacgata tgttttcttc aaaacaaccg accgattccg gcactcctgc
    11701 tggtattatt tcaggttcgc gcctcttcag gaggctcaag ataggtcaaa agttcttttt
    11761 ccggcctcgc aaaataaaaa ccctgcccgt aacgaatgcc catattgcga atatatttgt
    11821 aatcctcttt gttctcaacg ccctctgcca ccatctcaga accgttcatg cggccaaggt
    11881 gcaccatttc cttcacaatc tgctgattaa cgtgattgtc cgcaataccc cggacaagcc
    11941 ggagatcaat cttgaaaaaa tccggtctga gcgatgccat ttgaaatgtt ttcgctccga
    12001 tctcggtcaa atcatccagc gcgaaccggt atccaagcga ccggataaaa gtcacacccc
    12061 ttctaataaa ttcaaaatcg gatcgtttaa cgccttccgc gagctcaaaa acaatttttt
    12121 ccgagtactc tttgacaccg tcaagaaaaa cttccccttt tttccctttg tcgaaagctt
    12181 gttcaagcgt gggagactca atgttaagga aaagatattg cccctccgcc aaattcggaa
    12241 gcgcgccaat cgcgacctcc aggcaatgaa aatcaaaagg gaccaccgtc ccatcctcat
    12301 aagatttttg aaacatctcg aggggagtct ccttttcgct ccggacgagg gcctcgtacc
    12361 ctttaatatc ccaggtcgcg atatcaacaa taggttggta aacggaataa ataccactac
    12421 tgattttttt ctttttccca aacagattaa atttgaattt cataaatttc aacttacttc
    12481 ccgttcttga cttcaggcag gggccgaggc ccgcgacatg atttccgtca caaccttgcc
    12541 cacgcgacgg agatgcctgt cagataatgt cggatgaatg aggaacatga gacttgtctc
    12601 ggcaagtttt ctcgcgaccg ggaagcgtct caccggaccg agattttttt ttcggaatgc
    12661 tttttcaagg tagatctctc cgcaactccc gaccgaacaa aagatgcctt ttcgattcat
    12721 ggccctgaga attcgcgctc ttgaccatcc ctttttcagt ttttcaggac gcaaaaacac
    12781 ataatatttg tagaaactgt gccctgtttc aatcgtcggc atcgtgatac ggaaagcggg
    12841 aattttttta aaatagcttg tcagaatctc cgcatgggtc ctccgtttcc taacccacct
    12901 gcgcacttta cggagctgaa ctcggccgat ggccgcctgc atttcggtca ttcgaccatt
    12961 cgtcccaaat gaatcgtgaa cccactgaaa ccggggaaga aatttaaggc cagaggaagt
    13021 cccgtgccgc ttgccgtgat ccttgtaggt ccacgccttt ttccagatgg cttgatcgtt
    13081 ggtggtcagc atcccaccct cccctcccgt cgtcagaatt ttgtcctgac aaaaagagaa
    13141 ggccgcaacg tgtcccaggg aaccaacggg acgcccctga taagaagctc ccagcgcctg
    13201 cgcacaatct tcaatgactt tcaaaccccg ttgccgggca aaggccatga tcggcttcat
    13261 gtcgcagggc caccccgcca gatgaaccgc gataatcgcc cgcgtgcggg aagttaaaac
    13321 cgccttaagg gtgtccacgg ttatattctg actgacagaa tcaacgtcag ccacgatggg
    13381 ccacgcgcca cacatcacgg cacaactggc ggaagcaata aaggtcctgc ttgggacaat
    13441 cacatcatcg ccaggcccaa tccccagcgc ccgtaaagcc gtttcgagag ccacggtccc
    13501 attggccaag gccacggcat aacgacaccc cacaaacaaa gcaaactctt tctcaaaaag
    13561 gcgtccctcc gaacccgtcc agtaattaac cttgccggat ttcaatacgc gcgataccgc
    13621 cgctatttcg tctttctcaa aatgcggcca tgaaatcttt tgagactgct cgcgttcttt
    13681 catctttggc ctttaaacct ttctcatcac cctggcaggc acgccccaag cgaccacgtt
    13741 gtccggaata cttttattca caaaactgaa ggccccgaca accgaatttt tccctaccct
    13801 aactcccggc atcatcacac tatgacttcc gattttacag ttccgtccca gcgtgacaga
    13861 acctttctta ccatcgatag ttgaatgcga ataaatcgaa caatgagaac ccacctgaac
    13921 ttcatcttct atgaagaccc catgtttcgc atgaatgtag gtaaaggcgc ctatatcagt
    13981 attctttccc aatttaaaat tttttttccc ttggacgatc cagccatatc gggtgggtct
    14041 tccatccact attctggggc gtttccattt cgcaaagcgt ttttcattat tttgtttcat
    14101 gaattcaaat ccttccccgt gacagcctta tctgacgaac ctttgaactc ctgtgctgtc
    14161 gcctgtcccg cctggctgat tccttcccgt ttaaaaactt tccagattgt aagaaaaaaa
    14221 atctttaaat ccaaccagaa actccagtga tcgacatacc agacgtcata tttgaatttt
    14281 tcttcccagg aaatcgcgtt tcgtccgtgg acctgggccc agcccgtgat cccgggcatc
    14341 atgtcatgcc gccgcatttg ctccgacgtg taccggctca gatactgcat cagtaaggga
    14401 cgcggaccga tgaaactcat atctcccttc aacacattcc aaatttcggg gagttcgtcc
    14461 aggctcatcg aacgaaaaaa tcggcccacg aaactaagcc gttgtccatc cggcaggaga
    14521 tctccatgat tattttttgt ctcgtgcatc gtacgaaatt tatagagaac aaacggccgt
    14581 ccccggtatc ccggccggac ctgcttaaaa aaaatcggcc ggccttgggc gatccagagg
    14641 catgcgacac aaagtcccag aaccggagcg cccgcgacaa ggcaaaaaaa ggccagacaa
    14701 aaatcaatgg cccgttttat ttttaacaaa ggacgattcc gcatagaatt cgcctcatgg
    14761 gaaaagttaa aacacactga tctgcgggaa aaattccttt agccagcgat aaaccgattc
    14821 atcatccccc gcgagggcga aggagttgac tttctcaaac tgcttatcac tgatctcagg
    14881 gaccacccgg atggcggtca gcgccgcaat cttctcgaaa tctgttttgt cccatcgctc
    14941 ataccgcggg tcgtacaatt cttccgtaat tttctcaccg ggacgtaacc ctgtgtactc
    15001 aatccggatt ccttcttttt ccagtcctga taaccggatc gcaagacggg caaggtcctg
    15061 gatcttgacg ggagttccca tgtccagaat aaaagcggct ccattccccg taaaaaaagg
    15121 cgcctggagg accaggtgaa ccgcttcctg aagatccata aaatagcgtt ccacgtccgg
    15181 atgagttact ttcaccggac cgcctttttc tatcatgtct ttaaacaaag gaaccacact
    15241 gccgcggctt cccagaacat tcccgaaccg gacaaatgta atttttttat cctcccgatt
    15301 cggcgtttca cgcaaaacca gcagttcggc catccgcttg gtcactccca taacattgac
    15361 cggattcaca gctttatcgg tcgacaccag acaaaacttc ttgatgaaat ccttgcgttg
    15421 ggcgagatca ataacatttt ttgttcccag aacgttcgtc gaaacatact ccgaaggatt
    15481 ttgggcagaa atggggacat gcttgtaagc ggccgcgtga taaatcactt ccggcctttc
    15541 cctatcaacg attttttcca gcttaccgat attcctgacg tcgcacaaat aaagcacgta
    15601 ccggtcgcgg gaaagaagcc gtgacaattc ataatctaac cagaaaattc cggtttcatt
    15661 gacatccacc acaatgatct tccccgggcc atatcgcgca atttgtttga ccagttcttt
    15721 tccgattgag ccacaccccc cggtgacaag aaaccgtttt ccgttcagat ttttttccaa
    15781 aaaagcgata ttgggaacca cggggttgcg ccccaacaaa tcctgaacat caatctcccg
    15841 cgccaccgac gac
//
LOCUS       DAOSIO010000013        15100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_4072_length_15100_cov_4.522300, whole genome shotgun sequence.
ACCESSION   DAOSIO010000013 DAOSIO010000000
VERSION     DAOSIO010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 15100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15100
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4072_length_15100_cov_4.522300"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..922
                     /locus_tag="PK590_01130"
     CDS             95..922
                     /locus_tag="PK590_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOG23232.1"
                     /translation="MYEILSKTKLNNDVEEMVIRAPYVARRCEPGQFIILRVDDEGER
                     IPLTIAEYDSQKGTVSIIYQVVGFTTRKLSEKKAGEFIADFVGPLGKPTRLHAHKRVI
                     GIGGGVGAAPLYPQLRKLSEMGTEVDLILGARNKDLIILEEKFRTFCKNIYITTDDGS
                     YGMKGLVTDKLKELVEGGNQYDEVIAIGPLVMMKFVSLLTKQYNLKTSVSLNPIMIDG
                     TGMCGGCRVTIAGETKFACVDGPDFDGHQVDYDELLRRQGMYKTEENHACRIGLGKV"
     gene            925..2325
                     /gene="gltA"
                     /locus_tag="PK590_01135"
     CDS             925..2325
                     /gene="gltA"
                     /locus_tag="PK590_01135"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOG23233.1"
                     /translation="MAPNMNPKKVPIPEQSPAERVKNFNEVALGYTPEQAQEEASRCL
                     QCKARPCVEGCPVNVRIPEFVKKVAEGDFDGAYRILVSENCLPAICGRVCPQETQCEA
                     RCVRGKKGESVAIGRLERFVADYAMEKMSADAPRIERNGIKVAVAGGGPAGLTCAGEL
                     ARLGYEVTVFEALHALGGVLMYGIPEFRLPKKLVKKEIAGALKLGVKIEKNVIIGKSI
                     TVDELFQEGFQAVFIGCGAGLPSFMKIEGENLNGVYSANEFLTRANLMKAYDFPNSPT
                     PVKVGKRVAVVGGGNVAMDAARTARRLGAEEVYIVYRRSEKELPARLEEIHHAKEEGI
                     IFKFLTAPVKVLGESGWVTGMECVEMELGEPDASGRRRPVAKKGSHFVFPVETVIVAI
                     GGTPNPLIRQTTPGLETQKWGGIVVDEATMKTAKDGVYAGGDIVTGAATVILAMGAGK
                     KAARAIHENFQKKASG"
     gene            2470..2898
                     /locus_tag="PK590_01140"
     CDS             2470..2898
                     /locus_tag="PK590_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOG23234.1"
                     /translation="MKLITRNTDYAVRALCYLARQDQKSVPVSEMVTALKIPRPFLRK
                     LLQTLSTEGILDSFKGQAGGFALAKTPSKIRLTDLIRIFQGTVELNECVFRKKICPNR
                     STCKLKREIDAIEKYVLKRLSGVTVASLLGQAEHGEVERG"
     gene            2971..3765
                     /locus_tag="PK590_01145"
     CDS             2971..3765
                     /locus_tag="PK590_01145"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG23235.1"
                     /translation="MTQHPYQYIYGPVASWRLGRSLGIDPLSTDQKTCSFDCVYCQTG
                     RTKVLTLERKIFVPTEALLEEVRRVPAEPLDVVTFAGNGEPTLALNLGDMIRGIRTVR
                     SERIVVITNGSLIWRPDVREDLALADFVMVKLDAGSSDSLEAVNHPAEGIGFEEICEG
                     LRVFRKNYHGKLALQIMFVSSNKDLVADIVSLAQTIRPDEIEVNTPLRVCAVKPLTAE
                     EMVPITDLFRKTFTRIPVKSVYEEERKKSQPYCRQSTDRRRGQELP"
     gene            3887..4816
                     /gene="nadA"
                     /locus_tag="PK590_01150"
     CDS             3887..4816
                     /gene="nadA"
                     /locus_tag="PK590_01150"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOG23236.1"
                     /translation="MNQSHNKSSAEELLQEIAVLKEERHAVIIAHNYQPPEIQDLADH
                     VGDSLELSRLAAKTDARVIVFCGVKFMAETAAILSPDKTVLLPAREAGCTLADCATAG
                     RIREMKQKYPGALVVTYVNSSAEVKAESDICCTSANAVDVVKSVDPSKQIIFAPDYNL
                     GHYVSKVTNRRMILWDGCCPTHATLSTSGVEKAREKWPKAIFMMHPECKPAALELADY
                     VGSTSAMIRYAKQQPDGTEFIVGTEGGLLHKLEKENPGKVFHVASDFLVCPMMKMISL
                     GDVRDSLEKMQSVISVPETIRVKARAAVEAMLL"
     gene            4864..6633
                     /gene="ilvD"
                     /locus_tag="PK590_01155"
     CDS             4864..6633
                     /gene="ilvD"
                     /locus_tag="PK590_01155"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOG23237.1"
                     /translation="MKKAIHEGFEAIQKKGSRVVAEGKHMLPAAGLMVATGVISSIQD
                     RSRPFVTVINSYTTHIPGHVHLNILGEKVVQALKVKGYNVWYANIGAAICDGIAMGHF
                     GMKYSLPSRELIADQIESIIGAHPCDAWIGIGNCDKIIPAMLNSMVRLNIPSVYVSGG
                     PMLAGRNKSDLITVFEGVGQEAAGKISAGQLTEMANTACRTCGSCAGMFTANSMNCLA
                     EVIGLALPGNGSIPAAAWTDTKTLSWAVNPERIALVEKAADVLDNMLKKKIRPRDIVT
                     KKSIDNAFILDLAMGGSSNTVLHTLALACEAGIDYPLERINRLAKRTPNLCKVSPSRP
                     DIHMEDVHRVGGIAAILKAVVEDGRAPLNLDVKTCTGTLRDMVEKAPSADGDVIRIGD
                     RTFSKESGLAILYGNIAPQGAVVKTAGVEKDMMKFTGRAKCYDSQDEALEAILKGKVV
                     DGDVVVIRYEGPRGGPGMMEMLSPTSAIKGRGLRAALITDGRFSGGTRGLCIGHISPE
                     AAAGGPIAIIKNGDVIEIDAVKRTMELLVPAKEIKSRFRKLKPFKSKVTGGWLGRYSL
                     HVLSAHTGAVMDNSALFENKKQK"
     gene            6641..7129
                     /locus_tag="PK590_01160"
     CDS             6641..7129
                     /locus_tag="PK590_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOG23238.1"
                     /translation="MISEKMAKAINEQINKEIYSAYLYVGMAAYAGGLGLGGIANWFQ
                     SQAKEELGHAQKFMHYLSEQGSDLVLKAIAAPDQKFTSVAAAFDKTLEHEKEVTASIR
                     DLVTLAKKEADYATEIFLQWFVTEQIEEEASVSDIIQKLAIGGKMGNSLLMLDKTLGL
                     RK"
     gene            7148..8341
                     /locus_tag="PK590_01165"
     CDS             7148..8341
                     /locus_tag="PK590_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOG23239.1"
                     /translation="MKAIRIADDVWWVGAIDWNARHFHGHTYTTKRGTTYNAYLIVDE
                     KITLVDAVYGPFTDEMIQRIRTVIPVEKIDTIIANHIEPDHSGAYPELLKLCPNAKLY
                     GSARAGQGLERYYHQKFDFAPVKTGDKLSLGKKTLRFIEIPMIHWPDSMMTYLEEDQI
                     LFSNDAFGQHYATNERFDDEVPWEILMEEAAKYYANILWPFSHLIAKKLVELAEMNLP
                     IRMIATSHGVSWRKEPRKIITAYERWAANVTDPKIVIVYETMWGSTAKMARKIAEGVA
                     TSGVKCEIHDIAATDRTEVIYDMFSAKGYIVGSSNHDSGMLPNMVSFMEFLKGLHPKN
                     RIGCAFGSYGWSGIAIKKLEETLKESGVEIVQPALSVVYAPDEAQLAACHEFGRKIAE
                     FVKAV"
     gene            8346..8915
                     /locus_tag="PK590_01170"
     CDS             8346..8915
                     /locus_tag="PK590_01170"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG23240.1"
                     /translation="MKTFDAIEKRHSTREYKDQPVPKEMLEALVDAGRRAPSARAVEP
                     WEFVVVTDRGRLDILASLVSTGAFLKQATACIVVLCEDVKYFLEDGAAATQNILLAAT
                     DLGLGTCWIAGDKKEYAPDALKLLDVPPGYKLVSLVAVGWPAKSSHQHKKRKLKEVIH
                     WERFVERPRNKDPRIERVLEANSKLFPPK"
     gene            9186..10463
                     /locus_tag="PK590_01175"
     CDS             9186..10463
                     /locus_tag="PK590_01175"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOG23241.1"
                     /translation="MLSELSVKGKTIDLIVIGAGPAGLMAAISAASKGARVLILEGEA
                     SSGKKLLVTGGGHCNITNRAVSERDFYSDDPRTVRNVLRALPVSKTVDFFREWGLSLM
                     TEEDGKMFPRDRKAASVLGTLLGAARERKVNLLTRSEVLALHCRHGIFSTEGKGFSFS
                     SKAVIVATGGLSYPATGSRGAGYKIAESFGHSIVAPSPALVPFETADADWRGLAGITL
                     ETALGLFRRGQWFAESRGPLLFTHQGFSGPAVMNLASPWARFGGQGALTICANFLPQI
                     SEKSLRERLREKTVAKPAMTIKKFLSHDIPERLAGVLLKKANIVSEKQLANFSKKERE
                     LLFRMVRSCVLPVKGPLGYDRAEVTAGGIPLKEVNPKTLESNIAPGLFFAGEVLDVDG
                     RIGGFNLQWAWSSGYVAGCAAAKKLGQPIRSRL"
     gene            10596..11984
                     /locus_tag="PK590_01180"
     CDS             10596..11984
                     /locus_tag="PK590_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOG23242.1"
                     /translation="MRLFFFTCLMSVFFAVSGSAEELPPLPRGPMILTSATQAMQNAD
                     FWIRRIEGAERVVKTREEIEKFNEYIRSLVRENVDIFRLDFRRPGKQIADQLQLEFNT
                     LRNRKLFGVDNQYVQKSFFDDQIKPLVQIDKVPSTIRMKWGAAVRTTSVRALPTMVKL
                     LEDKGDVEFDQLQFTLIKLWTPVGIYHSSSNGQWLYIQAPYVRGWVKAKDIAVFPSRD
                     DLKKYAASDKFFVVTGESASVFKDPARTTLLQRASMGTVIPRAPIPSSQLAAGSSEPY
                     TVWMPYRKSDGSAGVAKAYISRKSDVQRNYPVYSQANILRQAFKLLGFRYGWGGQYNG
                     RDCSGFVHDVFLSFGIAFPRDSKQQSLVGIQLGFFEPFEEDIQKEKALGSLRPGLALL
                     KMPKHQMIYLGQIGKQHYVIHSTWAERTGQDPRKDEKNRINQVVVSDLNLNGNSYIGS
                     LFDRIVSINEIE"
     gene            11971..12537
                     /locus_tag="PK590_01185"
     CDS             11971..12537
                     /locus_tag="PK590_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23243.1"
                     /translation="MRSNKSFLPSWARGTLCLWFALVCVLGFPKDTWAEKFESAEYGF
                     AIEAPEGWQARPAVYEGVVATFWKEGSLATFHVIERDLGEAKTVADLEWKDLFAPKFD
                     EILIRQESETMVGGEKARYCIYKIRPGLFKQQMEGKHDFKYMNYIVIHENRLYSITFK
                     GLESSFGQDYSGFLESVRTIRFLDSQKP"
     gene            12569..12958
                     /locus_tag="PK590_01190"
     CDS             12569..12958
                     /locus_tag="PK590_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23244.1"
                     /translation="MNFQELVGEVQKIKFREIRSRTETYFEAVIPKQDLGSLETILQE
                     HFGKPLKPAGVFAEGEAKRYSKPYGGVRHEQTMYLRKLERSFEYAFLWPWGCGTLLTI
                     KVIQGEAPKGSPRELDFRAIWQSLWNK"
     gene            13014..13949
                     /locus_tag="PK590_01195"
     CDS             13014..13949
                     /locus_tag="PK590_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOG23245.1"
                     /translation="MKYAIGVDIGGTKIGVVLGNRNGALLVRREISTLLNQGARGCLN
                     QLVHTIEGIIRERGMTSRDLLGIGVGAPGAVNSRKGILPRSPNLPGWANIPLRGILAR
                     RFKLPVYLANDANAAALGELMFGAGKSARHFIYITVSTGVGSGIVIDGKLYEGAGFVA
                     GEFGHVSIVPDGLKCHCGRRGCLEAYASGTAIARFYGKSGKKKVEGAKGVGIAARLGD
                     KTAIKSYRKAAYYLGIGIAGLMNVLNPEVIVIGGGVLKSAPAIYWKEMIQSAKSHAWP
                     EAFCSTRVLKSTILDRSGDLGALALVFRNSAPGRT"
     gene            complement(13931..14004)
                     /locus_tag="PK590_01200"
     tRNA            complement(13931..14004)
                     /locus_tag="PK590_01200"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13968..13970),aa:Arg,seq:tcg)
     gene            14073..14996
                     /locus_tag="PK590_01205"
     CDS             14073..14996
                     /locus_tag="PK590_01205"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOG23246.1"
                     /translation="MPFADVWNGDRILKLHLKARKNFCEFFKNRYTVISGLIFFICGC
                     ATTTERGIDVEAARQSSSNYDEHAVGAKIHQEILSRFYPYTDPKVVKYVSHVGTNLAT
                     HASRKDLRYQYTILYDEKIYATSSPGGYIYVTTGMLNFLQNEAELAAILAHEIGRLQY
                     KDPRFTQTDDVVNVATQAGSQIAPMFGPIGSLAALGLVLLKAYVDSTQLTPDELLIES
                     DRRAMDYLLRSGYDPQSLMDTLEYFLRAGEKSIPLFYDYYQSRPITESRVLAVRKGFQ
                     KLPLSDKELKTRPLEYQEVTKGVREIYKDRY"
     gene            complement(15006..15087)
                     /locus_tag="PK590_01210"
     tRNA            complement(15006..15087)
                     /locus_tag="PK590_01210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15051..15053),aa:Leu,seq:tag)
BASE COUNT         3722 a         3415 c         4369 g         3594 t
ORIGIN      
        1 ggatcgggac gttcgattgc ctgaagccgg tgagcgtacc cggtttgctt gaccctctgg
       61 gtataattca tctaaccaat ttggaaatga tcccatgtac gaaattctct caaagacaaa
      121 actgaataat gatgtcgagg aaatggtgat ccgggcgcct tatgtggcgc gtcggtgtga
      181 accgggccaa ttcatcattc tccgcgtgga tgacgaaggt gagcgaatcc cccttacgat
      241 cgccgaatat gattcgcaaa agggcacggt atcgatcatc taccaggtgg ttggattcac
      301 tacccggaaa cttagcgaaa agaaagcggg ggaattcatc gcggatttcg tgggccctct
      361 tgggaagccc acccggcttc acgcccacaa acgtgtgatc gggatcggcg ggggtgtcgg
      421 cgcggcgcct ctttatcccc agttgaggaa actgagtgaa atgggaaccg aggtggatct
      481 tattctcggc gcgcgtaaca aggacctgat tatcctcgaa gagaaattca ggacattttg
      541 caaaaatatc tatatcacga cggatgacgg aagttacggt atgaaaggtc ttgtgacgga
      601 caagttgaag gagcttgttg agggcggcaa ccaatatgac gaagtgatcg cgattgggcc
      661 gcttgtcatg atgaaattcg taagcctttt gaccaaacaa tacaatttaa aaaccagcgt
      721 ttccctcaac ccgatcatga ttgatggtac cggaatgtgt ggcggatgcc gtgtgaccat
      781 cgcgggtgag acaaagtttg cctgcgtgga tgggcccgat tttgacggcc accaggtaga
      841 ttatgatgag ttactgcgcc gacaagggat gtacaagacc gaggagaacc acgcctgcag
      901 gatcggtctg ggaaaagtgt aaaaatggcg ccaaacatga atcctaaaaa ggtgccgatc
      961 cccgagcaaa gccccgcaga acgcgtcaag aatttcaatg aagtcgcttt aggttatacc
     1021 cccgagcagg cccaggaaga ggcgtcccgc tgtctccagt gcaaagcccg cccctgtgtt
     1081 gaaggttgtc cggtcaatgt cagaattccg gagtttgtca aaaaagtcgc ggaaggtgat
     1141 tttgacggag cttaccggat cctcgttagc gaaaattgtc tcccggcgat ctgcggccgc
     1201 gtatgtcccc aggaaaccca gtgtgaggcg cgttgcgttc gagggaaaaa aggggagtcg
     1261 gtggcgattg gaaggctcga gcggtttgtg gcggattacg cgatggagaa aatgtccgcg
     1321 gacgctcccc gaattgagcg aaacgggatc aaagtcgcgg tcgcgggcgg agggcccgcg
     1381 gggcttacgt gcgcgggaga actcgcgcgt ctcgggtacg aggttaccgt ttttgaagcg
     1441 cttcacgcgc ttggcggggt tttaatgtac ggaattccgg agttccggct tcccaaaaag
     1501 ttggtcaaaa aagagatcgc gggcgctttg aaactcgggg tcaagattga aaaaaacgtg
     1561 attatcggga aatcgatcac cgtggatgag ctttttcagg aaggattcca ggctgttttc
     1621 atcggttgcg gcgcgggact tccttctttc atgaaaattg aaggcgagaa cctgaacggc
     1681 gtttattccg cgaatgaatt tctgactcgc gcgaacctga tgaaggctta tgatttcccg
     1741 aattcgccga ctccggtgaa agttgggaaa cgggttgccg tggtcggcgg agggaatgtc
     1801 gccatggacg cggcgcgaac cgcgagacgg cttggggctg aggaagttta tatcgtctat
     1861 cggcgaagtg agaaagaatt gccggcaagg cttgaggaga tccaccacgc gaaagaagag
     1921 gggattattt ttaaattttt aacggcgccg gtaaaggtac ttggagagag cgggtgggtg
     1981 acggggatgg agtgtgtcga gatggaactt ggcgagcctg acgcgtccgg ccgccggcgg
     2041 ccggtggcta aaaaaggcag tcactttgtt tttccggttg agacagtgat cgtggcgatt
     2101 gggggaacgc cgaatcctct cattcgccag acgacgccgg gtcttgagac ccagaagtgg
     2161 ggcggcatcg ttgtcgatga agcgaccatg aagaccgcca aagacggggt ctatgccggc
     2221 ggcgatatcg tgaccggcgc ggctacggtg attttggcga tgggagccgg gaaaaaagcg
     2281 gctcgagcga ttcacgagaa ttttcaaaag aaagcctccg gatagggaag aatttttttt
     2341 gcttgataaa tgatactaaa gtagtatcat taaacatgat atagctgatt ttattttcca
     2401 tccctttgcc tcgtgagggg acggaaacgc gtctgaaacc cctctggagt tgtcgggaga
     2461 acaaggctga tgaagctgat tacgcgaaat acggactacg cggtgcgcgc tctttgttat
     2521 ctcgcgcggc aggatcagaa atccgtgcca gtttcggaga tggtgacggc gttgaaaatt
     2581 ccgaggccgt ttctgaggaa gcttttgcaa acgcttagca cggagggcat actcgattct
     2641 tttaaggggc aggcgggcgg tttcgccctt gccaaaaccc cctccaagat ccggttgact
     2701 gatttgattc gtatttttca ggggacggtt gaactgaacg agtgtgtttt tagaaaaaag
     2761 atttgtccga accgaagtac ctgtaaatta aaacgcgaaa ttgacgcgat tgagaaatat
     2821 gttcttaaac gactttccgg cgtgactgtt gcttcattgt tgggccaggc ggaacatggg
     2881 gaagttgaac ggggttaact ggtttgtgga gcttgtgtcg ggaagtataa tggctccggc
     2941 tttgagatac atgtttaatc caattccttt atgacacaac atccatatca atacatttac
     3001 gggcctgttg cttcctggcg gctggggcgt tcccttggga ttgatcctct ctcaaccgac
     3061 cagaagacct gttcgtttga ctgcgtctat tgccagaccg gacgaacaaa agttttaact
     3121 cttgagagga agatatttgt tccgacggag gcgttgcttg aagaagtccg gagagttcct
     3181 gcggagccgc tggatgtcgt taccttcgcc gggaatggtg aaccaaccct tgctttaaac
     3241 ctgggcgata tgattcgggg gatacggacg gtgcgttccg agaggatcgt tgtcattacg
     3301 aacggttctt tgatctggcg accggatgtc cgtgaagatt tggccctggc ggattttgtc
     3361 atggtaaaac ttgacgcggg ctcatccgac tccctggagg cggtcaatca tccggcggaa
     3421 gggatcggtt ttgaggaaat ttgcgaggga cttcgggtgt tcaggaaaaa ttatcacggg
     3481 aaactcgctt tgcagatcat gtttgtctcg tctaacaagg atctggtcgc ggatattgtg
     3541 agcctggctc aaacgattcg tcccgatgag atcgaggtaa acactccttt gcgggtgtgc
     3601 gccgtcaaac cgttaacggc tgaagaaatg gttccgatta cggatctttt ccgaaaaact
     3661 ttcacccgga tccccgtcaa aagcgtttat gaagaagaac gaaaaaagag ccagccgtac
     3721 tgtcggcagt ccaccgatcg tcgcaggggc caagaactac cctgaagggg tggtccgcct
     3781 gagcgatgac cggtgatggt ttaaagccaa aggtggccat aaagccatag agttcgttgc
     3841 aactcaggtt aaaaaaaagt aaaatgccat aattccggag ttaatcatga atcaatccca
     3901 caataaatct tcagctgagg agcttcttca ggaaatcgcg gtcctgaaag aggaacgtca
     3961 tgccgtcatc atcgcgcaca attatcaacc tcccgaaatt caggatctcg cggatcacgt
     4021 cggagactcg ctcgaactga gtcggctcgc ggccaaaacg gatgcccggg tcattgtttt
     4081 ttgtggcgtg aaatttatgg ctgaaaccgc ggccattctg tctcctgaca aaacggttct
     4141 tttgcccgcg cgcgaggccg gatgcacgct ggcggactgc gcgacagccg ggcggattcg
     4201 ggaaatgaaa caaaaatatc ccggcgccct ggtggtgact tacgtgaaca gttcggcgga
     4261 ggtgaaggcg gaaagcgaca tctgttgcac gtccgctaac gcggtggatg tcgtgaagtc
     4321 ggtggatcct tcgaaacaga ttattttcgc gccggattac aatcttggtc actatgtgtc
     4381 caaagtgaca aatcggcgaa tgatcctctg ggatgggtgc tgtccgacgc acgcgactct
     4441 ttcgacatcg ggtgtcgaga aggcccggga gaagtggccg aaggcgattt ttatgatgca
     4501 tcccgaatgt aagcccgccg cgcttgagct ggcggattat gtcggcagta catccgcgat
     4561 gattcgttac gctaaacagc agcccgacgg aacggaattc attgtcggca ctgaaggcgg
     4621 gcttttgcac aagttggaaa aagaaaatcc cggcaaggtc tttcacgttg ccagcgattt
     4681 tttggtctgc ccgatgatga aaatgatctc gcttggggat gtccgcgatt cgcttgagaa
     4741 aatgcaatcc gtgatttccg tgcctgaaac aattcgcgtg aaagccaggg cggccgtgga
     4801 agcgatgttg ctctaacggg aacgccggtc aaatctgcag gataattttt gagaggaaat
     4861 atcatgaaaa aagcgatcca tgaaggtttt gaagcaattc agaaaaaagg gagccgggtt
     4921 gtggcggaag gaaagcacat gcttccggcc gctggtttga tggtcgcgac gggggtgatt
     4981 tcttccattc aggatcgttc tcgtccgttt gtgacggtca tcaattccta cacgacgcat
     5041 atccccggac atgttcattt gaacattctc ggcgaaaaag tggttcaggc cctcaaggtc
     5101 aaagggtaca acgtctggta cgcgaatatc ggcgccgcga tttgtgacgg gatcgcgatg
     5161 gggcatttcg ggatgaaata ttcgttgccc tcaagggaac tgatcgctga ccagatagaa
     5221 tcgattatcg gggcgcatcc ttgtgacgcc tggatcggga ttggtaactg cgacaagatt
     5281 attcccgcca tgctgaactc gatggtgcgt cttaatatcc cttcagtcta cgtaagcggc
     5341 ggcccgatgc tcgcggggcg caacaaatca gatcttataa cggtatttga aggggtcgga
     5401 caagaagcgg ccggaaagat ttcggcgggc caactgaccg agatggccaa tactgcctgc
     5461 cgaacctgcg gaagttgcgc gggaatgttt accgcgaact ccatgaattg tctcgcggag
     5521 gtgattggtc tcgcgctccc cgggaacggc tccattcctg ccgcggcgtg gacggacaca
     5581 aagactcttt cctgggcggt aaatcccgag cggatcgcgc ttgtggaaaa agcggcggat
     5641 gtcctagata acatgctcaa gaaaaaaatc cggccgcggg atatcgtgac caaaaaatcg
     5701 atcgataacg cgttcatctt ggatttggcc atggggggat cctcgaatac ggtgctccac
     5761 acgctggcgt tggcttgtga ggctgggatc gattatccat tggagcggat taaccggctt
     5821 gccaaaagaa ctcctaatct ttgcaaagtg tcaccttcaa ggccggacat tcacatggag
     5881 gatgtccatc gcgtgggcgg gattgcggcg attcttaaag cggttgtcga agatggccgc
     5941 gcgcctctta atcttgatgt caagacttgc accggtacgt tgcgggatat ggtggaaaaa
     6001 gctccttcgg cggacggaga cgttatcagg atcggggatc gcacgttttc caaagaaagc
     6061 gggcttgcca tcctgtacgg aaacattgct ccgcagggcg ctgtggtcaa aacggccggt
     6121 gtcgagaagg acatgatgaa gtttacggga cgcgcgaagt gttacgattc gcaggatgaa
     6181 gcgctcgaag ccatcttgaa gggtaaggtc gtggatggtg atgtcgtggt gatccgttat
     6241 gaggggccga gaggaggccc ggggatgatg gaaatgttat ctccgacttc cgcgatcaag
     6301 ggacgtggac tacgcgccgc tttgatcacg gatggccgat tctcgggagg gacgcgggga
     6361 ctttgtatcg gacatatttc ccctgaagcc gccgcgggag gaccgatcgc gattatcaag
     6421 aacggcgacg tgattgaaat cgacgccgtg aaacgcacga tggaacttct tgttccggct
     6481 aaagaaatta aatcacggtt ccggaagttg aaacctttca aaagtaaagt gaccggtggg
     6541 tggcttggcc gttattccct gcatgtgttg agcgcgcata ccggcgccgt gatggacaat
     6601 agcgctttat ttgaaaacaa aaaacagaaa tgaggttgtt atgatcagtg agaaaatggc
     6661 gaaagcgatt aacgagcaaa tcaataaaga aatctactcg gcttaccttt atgtcggcat
     6721 ggccgcctat gccggaggtc tggggctggg aggaattgcc aattggtttc aatcgcaggc
     6781 taaagaggaa ctcgggcacg ctcaaaagtt catgcattac ctgagcgaac aggggagtga
     6841 tctggtcctt aaggcgatcg cggcgcctga tcaaaagttc acttcggtgg ccgccgcttt
     6901 tgataaaact ctggaacacg aaaaagaagt gaccgcttcc attcgtgact tggtaacgct
     6961 cgccaaaaag gaagccgact acgcgacgga gatttttctt cagtggtttg tgaccgagca
     7021 aattgaagag gaggccagcg tctccgatat tattcagaag ctcgcgatcg gtgggaaaat
     7081 ggggaattct ctcctgatgc tcgacaagac gctggggctc cggaaataaa tcggaaaaat
     7141 taaacgaatg aaggcgatac ggatagcgga cgacgtttgg tgggtggggg ccattgattg
     7201 gaatgcccgg cattttcacg gacataccta cacgacaaag cgcggtacga cctataacgc
     7261 gtacctgatc gttgatgaaa aaataacgct ggtagacgcg gtgtacgggc cgttcacgga
     7321 tgagatgatt cagcggatcc ggacggtcat tccagtggaa aagattgaca cgatcattgc
     7381 caatcacatt gagccggacc attcgggcgc gtatccggaa cttcttaaac tttgtccgaa
     7441 cgcgaagctc tacggaagcg cccgcgcggg acaagggctg gagcgctatt atcaccaaaa
     7501 gttcgatttc gcgccggtaa agactggtga caaattatca cttgggaaga agacgctccg
     7561 ttttatcgaa atcccgatga tccactggcc ggacagcatg atgacttacc tggaggaaga
     7621 tcagatcctt ttttcaaacg acgcctttgg acagcattac gcgacgaacg agcgatttga
     7681 cgatgaggtc ccgtgggaaa tcttgatgga agaggcggcc aaatattacg cgaacatcct
     7741 ctggccgttc agtcatttga tcgcgaaaaa gctagtcgag ctcgcggaga tgaatcttcc
     7801 gatccgcatg atcgcgacct cgcacggagt cagctggcgg aaagagccgc ggaaaataat
     7861 taccgcttat gagcggtggg cggcgaacgt gacggatccc aagatcgtga ttgtttatga
     7921 gacgatgtgg ggatcgaccg cgaaaatggc ccgaaaaatc gcggaaggcg ttgctacctc
     7981 gggagtcaag tgtgaaatcc acgacattgc cgcgacggat cggacggaag tgatttatga
     8041 catgtttagc gcgaaaggct acatcgtagg atcttccaat catgacagcg gcatgctccc
     8101 caacatggtg tcgttcatgg aatttctgaa aggtctgcat cccaaaaaca ggattggctg
     8161 tgcttttggt tcctacggat ggtcgggtat cgcgatcaag aaacttgaag agacgctgaa
     8221 ggaatccggg gtggagatcg ttcagccagc gctgtctgtt gtctacgctc cggacgaggc
     8281 ccagctcgcg gcttgccacg agttcgggcg aaaaatcgca gagtttgtga aggctgtgta
     8341 gttacatgaa aactttcgat gctattgaaa aacggcacag tacccgggaa tataaggacc
     8401 agccggttcc gaaggaaatg ctggaggcgt tagtggacgc gggccgacgc gctccgagcg
     8461 cccgggcggt tgaaccttgg gagtttgtgg ttgtaacgga tcgcggaagg ctcgatattc
     8521 ttgcttctct tgttagcacg ggggccttcc tcaaacaagc cacggcctgt atcgttgtgc
     8581 tctgtgaaga tgtgaagtat tttcttgagg atggggccgc ggccacccag aatattcttc
     8641 tcgcggcgac tgacctgggg cttggaacct gctggatcgc gggagataaa aaagaatatg
     8701 cccccgacgc gctgaagctc cttgatgttc ccccggggta taaattggtc agtctcgtgg
     8761 cggtcggatg gccggctaaa tcttcgcatc agcacaaaaa aagaaaattg aaagaagtga
     8821 ttcattggga aagatttgtc gagcgcccac gcaataagga cccccggatt gaaagggttc
     8881 ttgaagcgaa cagtaagttg tttcccccga aatagcaccg cggttttcgt ctgtttgtta
     8941 agacgaataa aattgatccg gccgaaagtg tcagcgtagt catggaatta tcggcatctt
     9001 gttgtaaccg ttttcgattc aacgaaacac agtaacaaaa aacaagttgc gttacgggat
     9061 atgcccaaag gggagccagg ctgtcaatgg cgtaaagatt tgagcttttt gaaattactg
     9121 acgaacccct gcgttttgtt aaataacgag gcgcggggtt ttgtcatttt aagggcgaac
     9181 tgaagatgct ttctgagtta agcgtaaaag ggaaaacaat cgatttgatc gtcattggcg
     9241 ccggtccggc gggccttatg gcggccatct cggcggcttc aaaaggtgct cgcgtattga
     9301 tcctcgaggg ggaagcgagt tccgggaaaa agttactcgt cacgggaggc gggcactgca
     9361 atattacgaa ccgcgcggtg agcgaacggg atttttattc ggatgatccg cgaactgttc
     9421 gcaatgtatt gcgggcgctt cctgtttcga agacggttga ttttttcagg gagtggggcc
     9481 tttcgcttat gacagaagag gatggaaaaa tgttccctcg ggaccggaag gccgcgagcg
     9541 ttcttgggac gctcctcggc gccgcgcggg aacggaaagt aaatcttttg acgaggagcg
     9601 aagttttagc gcttcattgc cggcacggga ttttttcgac tgagggaaag ggattttcgt
     9661 tttcctcaaa agccgtgatt gtcgctaccg gcggattatc gtatcccgcg accggaagtc
     9721 gaggcgcggg ttataagatt gccgaatctt ttgggcattc aatcgtggcc ccttcaccgg
     9781 cgcttgttcc cttcgaaacg gcggatgctg attggcgtgg cctcgcgggg ataactttgg
     9841 agaccgcgct tggattattc cgccgcggac agtggtttgc cgaatctcga gggcctcttc
     9901 ttttcacgca tcaggggttt agcggcccgg cggttatgaa tctcgcttcc ccctgggccc
     9961 gattcggtgg ccagggagcg ctgacgattt gtgccaattt tttaccacaa atcagtgaaa
    10021 aatctttgcg ggaacgtttg cgggaaaaga cagtcgcgaa accggccatg acaatcaaaa
    10081 aatttctttc tcacgatatc ccggaaagac ttgccggagt tctcttgaaa aaggcaaata
    10141 ttgtttcgga aaaacaactg gcgaattttt caaaaaaaga aagggaactg ctttttcgga
    10201 tggttcgttc ctgcgttctt cccgtgaaag ggccgcttgg gtatgacagg gcggaggtca
    10261 cggcgggcgg gattcccctg aaagaggtga acccaaagac gcttgaatca aacattgcgc
    10321 ccgggctttt ttttgccgga gaggttttgg atgtggatgg ccggatcggt ggatttaatt
    10381 tgcaatgggc ctggtcgagc gggtatgtgg ccgggtgcgc cgccgcgaaa aaattggggc
    10441 aaccgataag gtcccgactt tgaattatga tggaaaatgg gccgttttcg ggggcatcaa
    10501 acgttgaagg ttaagacttt gccggtaacg gtcgaaattt atcgggacat tccgaaacat
    10561 ctgccataaa atattgatcc aaaatgagga aaattatgcg cctattcttt tttacgtgtc
    10621 tcatgagcgt ttttttcgcg gtatcgggga gtgccgagga attgcctccg cttcccaggg
    10681 ggcccatgat tttgacgagc gcgacacaag cgatgcaaaa cgcggatttt tggatccggc
    10741 gaattgaagg cgcggagagg gtcgttaaaa cgcgcgagga gatagaaaaa ttcaacgaat
    10801 atatccgtag cctcgtcaga gagaatgtgg atattttccg actggatttt cgccgaccgg
    10861 gcaaacagat tgccgaccag cttcagcttg agtttaacac gctcagaaac cggaaactat
    10921 tcggggtcga taatcaatat gtccaaaaaa gtttttttga cgaccagatc aagcctctcg
    10981 tccagattga caaggtccct tccacgatcc ggatgaaatg gggagccgcg gtccggacca
    11041 cgtccgttcg ggcgctcccg accatggtga aattactcga agacaagggg gatgtggagt
    11101 tcgaccagct tcagttcacg ctcatcaaac tctggacgcc cgtcggcatt tatcacagct
    11161 ccagtaacgg acaatggctt tacatccagg cgccgtacgt gcggggatgg gtcaaggcga
    11221 aggatatcgc cgttttcccg tctcgtgatg accttaaaaa atacgcggct tccgataaat
    11281 tttttgtggt cacgggcgaa agtgccagtg tttttaaaga tcccgccaga accacgttgc
    11341 ttcagcgggc gtccatgggg acggtgattc ccagagcgcc cattccatcg tcccaactgg
    11401 cggctggatc cagtgaacct tacacggttt ggatgcctta ccgtaaaagt gacggaagcg
    11461 cgggagttgc gaaggcttac atcagcagga aaagcgatgt tcagagaaat tatcccgtgt
    11521 attcccaggc caatatcctg cgccaggcgt tcaagttgct tggatttcgg tacggatggg
    11581 gtggtcagta taatgggcgc gactgttcgg ggttcgtgca tgatgttttt ttgagtttcg
    11641 gaattgcttt cccgcgagac tccaaacaac agtccctcgt tggaattcaa ctcggatttt
    11701 tcgaaccctt cgaagaggat atccagaagg aaaaggccct tggttctttg cggccggggt
    11761 tggctcttct aaaaatgccg aagcatcaaa tgatttatct ggggcaaatc ggaaaacagc
    11821 actatgtgat tcattccacg tgggccgaac gcacgggaca ggaccccagg aaagatgaaa
    11881 aaaaccggat taatcaagtg gtcgtctctg accttaatct gaacggaaat tcctacattg
    11941 ggtcactgtt cgacaggatt gtctccatca atgagatcga ataaatcatt tctcccaagc
    12001 tgggctcgcg ggacgttgtg cctgtggttc gcgctcgttt gtgttctggg attcccgaaa
    12061 gatacgtggg ccgaaaaatt tgaatccgct gagtatggtt ttgccatcga ggcgccggaa
    12121 ggatggcagg cgcggcccgc ggtttatgag ggagtggtcg cgactttctg gaaagaagga
    12181 agtttggcga cttttcatgt gatcgagcgc gacctgggtg aggcaaaaac cgtggctgat
    12241 cttgagtgga aagatctttt tgccccaaaa tttgatgaaa ttctcattcg ccaggaatcg
    12301 gagaccatgg ttggcggaga gaaggcaagg tattgcatct ataaaatcag gccgggtttg
    12361 ttcaaacaac aaatggaagg gaagcatgat tttaaataca tgaattacat cgtcattcat
    12421 gaaaacaggc tctattccat tacgtttaaa gggctggaat cttcgttcgg ccaggattat
    12481 tccggatttc ttgaatcggt gcgaacgatt cgctttttag actcacaaaa gccgtgacgg
    12541 ctcccaacgg aaagttagag gttacgtcat gaactttcaa gaactggttg gagaagtcca
    12601 aaagataaag ttcagggaaa ttcgctcccg cacggaaacg tattttgaag ccgtcattcc
    12661 caaacaggat ttaggctctc ttgaaacgat cctccaggaa cattttggaa aacctctgaa
    12721 acccgcgggt gttttcgcgg aaggagaggc taaacggtat tcaaagccct acggcggagt
    12781 tcggcatgag cagaccatgt acctccggaa gcttgaacgc agtttcgaat atgccttttt
    12841 atggccttgg ggttgtggca ctctgcttac catcaaggtg atccagggcg aggctcccaa
    12901 aggttctccc agagaacttg atttccgggc catttggcaa tctttgtgga ataaataatt
    12961 ttttcttccc gcccgcggtt gaattccagt gacttcacag tagaataacg cctatgaaat
    13021 acgctatcgg tgttgatatc gggggaacca agattggtgt ggttcttgga aatagaaatg
    13081 gggcacttct tgtccgacgc gaaatttcga cccttcttaa tcaaggcgcc cggggttgtc
    13141 taaaccaact tgttcatacg attgaaggca taataaggga aagagggatg acctctcgag
    13201 atcttcttgg gatcggcgtg ggggcacccg gcgctgtaaa ctcgcgaaag ggtattctgc
    13261 ctcgttctcc taatctgccc gggtgggcca atattccgtt gcgtggcatt ctggcaaggc
    13321 gattcaagct tccggtatac cttgccaatg acgcgaacgc cgccgccctt ggagaactga
    13381 tgtttggcgc ggggaaaagt gcccggcatt tcatttacat cacggtcagt actggggtgg
    13441 gaagtggaat tgtgatagat gggaaacttt atgaaggcgc gggttttgtt gccggagaat
    13501 tcgggcatgt cagcattgtt cctgatggac taaagtgcca ctgcggccga cggggttgtc
    13561 tcgaggcata cgcttctggc acggccattg cacgttttta tggcaaatca ggaaagaaaa
    13621 aagttgaggg cgcgaaaggg gtcgggattg cggcccggtt gggtgacaaa accgcgatca
    13681 agagttacag gaaagcggct tattatcttg gaatcggaat cgcgggtctc atgaatgttt
    13741 tgaatccgga agtgatcgtg attggcgggg gagttttgaa atcagccccc gcgatatact
    13801 ggaaagaaat gattcaaagc gcgaaaagtc acgcgtggcc cgaagcgttt tgcagcaccc
    13861 gagttcttaa gtccacgatt ctcgaccggt ctggcgatct tggtgcgttg gcgctggtat
    13921 tcagaaacag cgcgcccggc aggacttgaa cctgcaaccc tctgctccga aggcagatgc
    13981 tctatccaat tgagctacgg gcgcatttta aattttcaga tcttcatcga aatgggatct
    14041 tctaagtggc caccagaaat tattgaggaa aattgccatt tgctgacgtc tggaatggag
    14101 atcgtatcct gaaacttcac cttaaagcaa gaaagaattt ttgtgagttt tttaaaaaca
    14161 gatacacggt tatttcgggg cttatttttt ttatttgcgg gtgtgccaca acgacggagc
    14221 gggggattga cgtggaggcg gcgcgtcaaa gttcatcgaa ttatgatgag cacgccgtgg
    14281 gagcgaaaat tcatcaggag atcctctcgc ggttttatcc ttacacggat cccaaagtcg
    14341 tgaagtatgt gagccatgtt gggacgaatc tggcaaccca cgccagccgg aaagacctcc
    14401 gttatcaata taccattctc tatgacgaga agatttacgc gacatcatct cccggcggat
    14461 atatttatgt gacgaccgga atgctcaatt tcttgcaaaa tgaggcggag cttgccgcta
    14521 ttctcgcgca tgaaatcgga aggcttcaat acaaggaccc tcggtttacg caaaccgatg
    14581 atgtggtgaa tgttgccacg caggcgggtt cccagatcgc gccgatgttt ggtcccatcg
    14641 gatcgcttgc cgctcttggg cttgtcttgc tcaaggcgta tgttgacagt acccagttga
    14701 ctcccgatga attgcttatc gaaagtgacc ggcgggcgat ggactatttg cttcggtcgg
    14761 gttatgatcc ccagtcgctg atggataccc tcgaatattt tttacgggcg ggagagaaat
    14821 ctataccgtt attttatgac tattatcaat cacggccgat tacggaatcg agggttttgg
    14881 ccgtccgtaa aggatttcaa aagcttccac tttctgataa agagctcaaa actcgaccgc
    14941 tcgaatacca agaggtcaca aaaggggtga gagagattta caaggataga tactaacgaa
    15001 actaatgcgg ctagagggag ttgaaccccc acccctttcg ggacaagatc ctaagtcttg
    15061 cgcgtctgcc agtttcgcca tagccgcata aaatttcgta
//
LOCUS       DAOSIO010000014        14616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_4226_length_14616_cov_4.311448, whole genome shotgun sequence.
ACCESSION   DAOSIO010000014 DAOSIO010000000
VERSION     DAOSIO010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 14616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14616
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4226_length_14616_cov_4.311448"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PK590_01215"
     CDS             complement(<1..575)
                     /locus_tag="PK590_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 family protein"
                     /protein_id="HOG23247.1"
                     /translation="MRAVVKNTGFDNLKYLNEQSAAILERIERFSDKLYLEFGGKMIG
                     DYHAMRVLPGFDPNVKMRLLHKLRDKAEIVMCIHAGDIERRKMRADFGITYDADIFRM
                     IDEYRGWELDVAAVVITRFAEQPAAKIFKNKLERRGIRTYTHRFTKGYPTDVDTIVSD
                     EGYGANEYIVTKKPLVIVSGPGPGSGKLATCL"
     gene            complement(679..954)
                     /locus_tag="PK590_01220"
     CDS             complement(679..954)
                     /locus_tag="PK590_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOG23248.1"
                     /translation="MPTYEYECSACGHQFEKFQAMNDQPIRKCPACGKKKVQRMISGG
                     AGVLFKGSGFYQTDYRSQGYRESAKKDKAPTSTPTCSGACDSCPAKN"
     gene            complement(1125..1919)
                     /locus_tag="PK590_01225"
     CDS             complement(1125..1919)
                     /locus_tag="PK590_01225"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOG23249.1"
                     /translation="MKFRFNICLGAGVLFFCFLTMGVHARDPEKIFVASEKPFGTFLL
                     GEKIKFKVTYAGITVGEAESWVKEIVRINGRDAYHIEINIRSKFVLDWIYKVRDTHHS
                     YVDVEKLHSLRYEKVLHEGRYWTDEVMEYDPSKNVGRFFSKKDNSRKEMLIPKNVQDQ
                     ISCGYYYRTLDVRPGTKAVIPVNADEKNWDLEVLAHNVEEMKIDGMGIFQALEVEPII
                     MFEGFFVRRGKVRGWMSMDERRIPLKLKVKVPVLGDVTATIVDYQP"
     gene            complement(2094..2447)
                     /locus_tag="PK590_01230"
     CDS             complement(2094..2447)
                     /locus_tag="PK590_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOG23250.1"
                     /translation="MRIPSFMLAGETSLAETAAILEMSSLFIGNDGGLVHLARALEVP
                     LAAFYGPVPPEIYGPYPGSENAIALFKRDLACRPCYHKFRFKNDCSTVACLKTLAPEE
                     AFKQMEPLLQTMSRG"
     gene            complement(2473..3192)
                     /locus_tag="PK590_01235"
     CDS             complement(2473..3192)
                     /locus_tag="PK590_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOG23251.1"
                     /translation="MKNIRSVLIVHPYGIGDLLFVTPVLRALRLLPTVGTVDLLLGSR
                     TREVVEHNPHVDEIFVLDKDKLRRQTRFENFRELLDLGLKLRARRYDLLLDFSLRPEN
                     AFWAFFFLGIPKRAGFAYKRRGIFHNIRYPLPEGYSRQHAADFYAGLAELAGIPVRDR
                     FLEYYFPPREKREIEIKIKDRLAGIGKNFITVSPGGGDSWGREAVFKRWPVKYFAELI
                     GRLQTKLGFKGGLYHRCQVRG"
     gene            complement(3251..4297)
                     /locus_tag="PK590_01240"
     CDS             complement(3251..4297)
                     /locus_tag="PK590_01240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOG23252.1"
                     /translation="MKNITLPDSPRILVTRTDRIGDLVLTTPVFKALRDQFPKAWIAA
                     LVFIEHREVVEGNPFLDEVILYDKQGLEKNIWGQLGFARRIRKKKFDLVIHCHGTNRM
                     HLVAWLAGIPARIGYDRRAPWALTHVKEYDKKFGEKQEAEYLFDLLAPLGITRPEVLE
                     SFFPVTDRAKRSFDHLMFHLKIPQGKPWVILSPSASDVTKMWPAGKFSELATRIHHDM
                     DGIIMAIGSAKDRAIVESVREKSNVPIMDLGGRLTLGMLAALLEKSAILVSNDSGPVH
                     IASAVGCPVVSIFGRYEPGLGPNRWRPLGRNCHVVARDISEVPCSERKFTYIDEITVD
                     AVYAAARDLLTRSV"
     gene            complement(4294..5316)
                     /locus_tag="PK590_01245"
     CDS             complement(4294..5316)
                     /locus_tag="PK590_01245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOG23253.1"
                     /translation="MKNPESILVVTLSNIGDVILTTPVMAALRLKFPDAKMTVVVGPK
                     AKPVLEKSGDISRIIVYDKKATLPGKVAFLRELWKDRYDMVVDLRNTAIPFLVFARKR
                     SPLFRAYTKINMRERHLEVLRMAGIRNGTKGEETFFSGNTRLPNFQFFSSADELSVFS
                     KLGSIGIQGKSGWILVAPGAASERKRWPPELFREVVRNLVLRTRKDVFLVGSRDERPI
                     AEVVARGMPASVKILSGETSLSETAFLVSKASLVISNDSAIMHLGFEIKTPTVGIFGP
                     TDHEKYGHVHPSFRIARENPTQCHCNSHRLPYAERSCFHGLTPEKVLCLCNELLNRDT
                     PAARPS"
     gene            complement(5313..6380)
                     /locus_tag="PK590_01250"
     CDS             complement(5313..6380)
                     /locus_tag="PK590_01250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOG23254.1"
                     /translation="MKILVVMKNWLGDLLFQMPALDLIKQKYPSAEIFCVAPERCREI
                     LEANPAVSGFIAFDEKSTHRFWFHRIGFVFHLRKLGPWQEGYLFHRSRSRAAFLWLAG
                     VKKRIGFGRGRKFFLAESLKEPAEKLHQLDYFMELMKGAGYQVPVSPKYRFYFREGDE
                     VQALKILRQEGLAEGARYVCFHLGANWEPKRWPVSHFAKLAFKIFGKWGVPIVVTGSA
                     GDRAIFENFRAEIGRLTGNGELDPAVVDLVGKTALRISAWIYKNAMCLVTGDSGPMHM
                     ASGAGARVVALFGPTDPHLTGPRGIGENRVLQYVPSGYSIPFFGKELPEEGWLSKITP
                     EAVFEAVEQLGAFPPQPGQNL"
     gene            complement(6475..8571)
                     /gene="waaF"
                     /locus_tag="PK590_01255"
     CDS             complement(6475..8571)
                     /gene="waaF"
                     /locus_tag="PK590_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HOG23255.1"
                     /translation="MKILQLLPALEIGGVERGVVDMARSLKEEGHETVVVSSGGTLVA
                     ELQRMGVPHYGLPVHQKSIVSLLLVDQLVKIIERERIDIVHARSRVPAWIGWLASRKT
                     GIPFITTCHGHYSVHPMSFVMGYGKRVIVISNAIGRRMIDEFRVPQDRIRLIPRGVDL
                     SQFVFSLKRFEEKPKRFRIIHVGRFSPNKGQVEFLKALHLLRSRFPNFEALIVGAENR
                     GRTRYTKLIHQTIKQFGLDTCVKVLGPSRDIPGLLAESDLLVLSSTLPEPFGRVIIEA
                     GAVGIPVAATWLGGVPDIIDHGRHGYLFPPKNVPAMAEAMHAMLTDRPWAKECAIALR
                     KKIEEKFNVEQMVKKTLEVYLEVRKKKKILVIKLGAMGDLVLVVPSLRMLKNRYPDSS
                     ISILVDKKLAPIISNCPYLDDLILVDRKKLGNPLYLLKTARRIRQENFDMSVDLHNNK
                     YTHLLAYLGGVVQRCGFARGFWGRLLNRSDKTFGLADTPVRHQFRVLAKAGVQDFEDE
                     LELWPKKERIEKAEQTLLALDLKADTRVIGFVMGSSPKWPTKRWDPQFFKKLAEKIFD
                     HYDARILLLGSPGEEGLADVFRDFDPDRVVNLIGKTTLEDLPAFFQKLHFVVSGDTAL
                     LHVAAAMHVPTAAIFGPTDPKRHVPPSRKIAIFTKNLSCQPCYQGRCKEKEMMGCLNH
                     VTVDEVFDAARRFLDL"
     gene            complement(8738..10687)
                     /locus_tag="PK590_01260"
     CDS             complement(8738..10687)
                     /locus_tag="PK590_01260"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HOG23256.1"
                     /translation="MEKILDWIAYGVARSLIFLFGKIPIETSLGIGDAIGKTVFYFSK
                     KRRVAYVNLKNALGTRLSPRERWKTVRDHYGHIGQNIVEVMSFPKLTPRHIEDHIKIH
                     HLERFEDLVKSGRGGVLLTGHFGNWELLQVVAGLRGTPIYVLARDQKFPKVNELLNQL
                     RETHGSVAVGRGVGVRALIKALREKKMIGVLGDQSAGKTEGIILPFFGKKTSVPTGAF
                     ELAIRTDSLLMPSYMVRIDRSRHEIFVEETLQDDPSADTETRVRSLARQYLDSLERFI
                     SQYPGQWLWENKRWKYSWSRKIVILSDGKAGHFKQSDVVAEMMSSLTEFHGRAGLEFQ
                     TEKIHIEYRSSLHRALLFLMAPFLKPWIQGRIEWLRIFLKPQCHQAVEEATADFIIAA
                     GSGLAPVQQILARETGAKTIVIMKPPFPYSLMNYDLAVVPAHDDGILPKNSTRCVIMP
                     SGYRPPDRSGDTAILRTQLGETGKARIAVFLGGKTRGFDITVPDVEKTAMILRRICRK
                     SAEYMLTTSRRTSRMTEQFLKSVLAQDPACRYLVIANEDNPPFAAGGMMQLADLVIVT
                     EDSLAMISEAVGAGKKVIVLHLGEGELPAKHYRFHQILIQRGLVTMSGLEDLDRTVAE
                     LMKKEVPPMLDRERNALLQKLGSLL"
     gene            complement(10687..11733)
                     /gene="lpxK"
                     /locus_tag="PK590_01265"
     CDS             complement(10687..11733)
                     /gene="lpxK"
                     /locus_tag="PK590_01265"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOG23257.1"
                     /translation="MENLKNYFRLVAEGKCRDAASFCLCPLLSVVSFVYGVGVRGLRW
                     AHEKKYLKRSKLPFPVISVGNLTWGGTGKTPLVEFLARKISAFRKTPLILTRGYNHDE
                     VEEFRHHMPTVVVGVGRDRVKTALGIVKEKRVDVAILDDGLQQVAVERDVEIIVVNSL
                     NPFGNQHLIPRGILREPMSVLKRATVVVLTHVNLVPPEQLKSLREQIQSLSPRAHFVE
                     AYMEPLFLYRAKNRSRVSLEKMRHKKVATFAAVGTPRSFQLVLQHASIKTARNFEFLD
                     HHEYKESELKEIKEISRSADVDEIITTEKDFYRSRDLITQVLNPLVLAARLRIRSGEE
                     ILTDRLLRLLGIKR"
     gene            complement(11681..12982)
                     /locus_tag="PK590_01270"
     CDS             complement(11681..12982)
                     /locus_tag="PK590_01270"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HOG23258.1"
                     /translation="MKIRQAEDPKRLWRERWGNLSGNRKKSPDSGKTVWLHAVSVGEV
                     NAVEKFVQELLERDRDLDVFLTTVTPTGQRIAKKMEGPRVRVAYFPFDISFVVRRFLD
                     EIQPVAVVLAETEIWPNLLIEVKRRNIPVGVVNARLSERSFKRYRAAGFLLSGLWKKI
                     DFVLAQTREDARRFRELGVFEKSVREMGNMKFDQVELERGKSLSPDLIRERFGVNGED
                     LVLIAGSTHPGEEKILADVFLELRKRFPALKMIVAPRHIERSGRLLKDLSTGSCRIVL
                     ASRKPAPGSFDVLVLDQLGILKELYQIADLVFMGGSLVPHGGQNPVEAARFARAVMHG
                     PHVFNFNMIYRELGQGGGAFLVREPGDLKAMSEKFLSDPALRREAGVKANQIVNRLKG
                     ASKCQAEWVLMFLASRMASERKKTDGESQELFSAGGRGEMP"
     gene            complement(13091..13975)
                     /gene="xerC"
                     /locus_tag="PK590_01275"
     CDS             complement(13091..13975)
                     /gene="xerC"
                     /locus_tag="PK590_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOG23259.1"
                     /translation="MATDQRVEDFLGFLTTEKGASIHTVKNYAIDLREFVKFLSEKDL
                     AEITYLDIRSFLAFLKTREYSKSSIARKLACLRSFFKYLTRENVLTQNPAAGIATPKK
                     EKRLPEFLNPDEITKLLEAPAKVSWEEKRDKAILEMLYSSGFRVSELVGLNHDDCDLF
                     AGWVRVRGKGKKERIVPVGQTAISCLKRYLDEKPPKEPQHAIKKPLFVSRLGGRLTDR
                     SIRRMILKYVKRVGLGKEVSPHMLRHSFATHMLDRGADLRSVQELLGHANLSTTQIYT
                     HVTTQRLKDAYATAHPRA"
     gene            complement(13980..>14616)
                     /locus_tag="PK590_01280"
     CDS             complement(13980..>14616)
                     /locus_tag="PK590_01280"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HOG23260.1"
                     /translation="NDASLVKLLEEKGIGRPSTYAPTISTLLNRDYCEKKGGALIPTE
                     LGEMITDLLVEYFPKVLEAEFTALMEEGLDEVEEGTREWKKVLYDFYGPFNELLIKAR
                     EEMKNFKREPVPTEFKCDICGKQLLIKFGRFGKFLACSGFPDCKYTRSIPTGFFCPEP
                     GCGGELVKRKAKGRRAFYGCSNYPQCTYTVQQLPKKGENSEPSSDPTSHPT"
BASE COUNT         3702 a         4148 c         3277 g         3489 t
ORIGIN      
        1 agacaagtcg ccagtttgcc gctaccgggg cccgggcctg aaacgatgac aagcggcttc
       61 ttcgtcacaa tgtactcgtt ggcgccgtac ccctcatcgc ttacgatcgt atccacgtcg
      121 gttggataac ccttggtaaa ccggtgggtg taggtcctga tcccgcgccg ctcaagtttg
      181 tttttaaaaa ttttagcggc aggctgttcc gcgaaacgcg taataacgac cgccgccaca
      241 tcaagctccc acccgcgata ctcatcaatc atgcggaata tatcggcgtc atacgtgatt
      301 ccgaaatccg cccgcatttt ccgccgctca atatcacccg cgtgaataca catgacaatt
      361 tcagccttgt cgcgaagctt gtgaagaagc cgcattttca cgttaggatc aaaacccggg
      421 aggacccgca tcgcgtgata atcaccgatc atcttccccc cgaattcaag gtagagctta
      481 tcgctaaatc gctcaatacg ttccaggatg gcagccgact gctcattgag atatttgaga
      541 ttatcaaaac ccgtattctt caccaccgcc cgcatcacgc ctccctgttt ttgtcgcaga
      601 acgccctaac tcgaaatctt cgtaatccgt tgaccgttac acatcacggg atcgtttgtg
      661 ttttgaaatg acctgccgct agttttttgc cgggcacgaa tcgcaggcgc cagaacaagt
      721 cggagttgac gtcggcgcct tgtctttttt cgcggattcc ctgtacccct gggagcgata
      781 atccgtctga taaaatccgc ttcccttgaa taaaactcca gccccacccg agatcatacg
      841 ttgaaccttt tttttcccgc aggccggaca cttccggatt ggctggtcgt tcattgcctg
      901 aaatttttca aattggtgcc cgcaggcact gcattcgtat tcatacgtag gcatatttct
      961 ctaatccctt tctttttcca tgaagcgccg attataggga aaaaaaactc caggggtact
     1021 attttttgcc agaggccgtc tcataaaaac cgccatcccg ccactaccca agcaaccgaa
     1081 agatggaacc caaaaactaa ttccgcaaaa tcggctccgc cagctcaggg ttgatagtca
     1141 acgattgtcg cggtcacatc tcccaaaacg ggcaccttta ctttaagctt caagggaatc
     1201 cgccgctcat ccatactcat ccagccgcga actttcccgc gtcgcacaaa aaacccctcg
     1261 aacatgatga tgggctctac ttcgagggct tgaaaaatgc ccattccgtc aatcttcatt
     1321 tcttcaacgt tatgcgccaa aacctcaaga tcccagttct tctcatccgc gttcacggga
     1381 ataaccgctt tggtccccgg cctaacatcc agtgtccgat agtaatatcc gcacgaaatc
     1441 tgatcctgga cattctttgg aatcaacatt tctttgcggc tgttgtcttt tttggaaaaa
     1501 aatcttccca cgttttttga cggatcatat tccatgacct cgtccgtcca gtaacgtcct
     1561 tcgtgaagga ccttttcgta gcgtagcgaa tgcagttttt caacatcaac gtaagagtgg
     1621 tgtgtgtcgc gaactttgta gatccagtcc agtacaaatt tagagcggat attaatctcg
     1681 atgtgatagg catcacggcc gttgatccgg acaatctcct tgacccagga ttcggcttca
     1741 cccacggtaa tccccgcgta agtcacttta aatttgatct tctccccgag gagaaacgtg
     1801 ccgaatggtt tttcggaagc cacaaagatt ttttcgggat cacgggcgtg aactcccatc
     1861 gtgagaaaac aaaaaaacag aactcctgcc cccaggcaaa tattaaatct gaatttcatc
     1921 gcatctccgt ttcaaaaaat ggtcaaggct cattacggac cggggaattt cttcccgcga
     1981 gatcctggat ttttacacaa tctttcgtaa tcccaaacaa cacgaaactt ccgttatcgc
     2041 aatcgctaca aatcccccgc caatcccgaa catttgccgc tctggatttt attttaacct
     2101 cgggacatcg tctgaagtaa aggctccatc tgtttgaaag cctcttcagg cgccagggtt
     2161 ttcagacagg caaccgtgga acaatcgttt ttgaagcgaa atttatgata acaggggcgg
     2221 caggcaagat cccgcttgaa aagggcaatt gcattttcag accctggata aggcccgtaa
     2281 atttctgggg gaacgggccc ataaaaagcg gcgagaggaa cttcaagagc ccgagccaaa
     2341 tgaaccaggc ccccgtcatt tccgataaaa agcgaactca tttccagaat cgcggccgtc
     2401 tccgccagag aggtttctcc agccagcatg aacgatggaa tcctcaaaga atctttaagc
     2461 tcctgacaaa gttcaacctc ggacctggca cctatgatac aaaccccctt taaaaccaag
     2521 cttcgtctgc aggcgtccta tcagctccgc aaaatacttg acgggccagc gtttaaacac
     2581 agcctccctt ccccaggaat ctccccctcc gggactgacg gtaataaaat tttttccgat
     2641 tccggcaagc cggtctttta tttttatttc aatttctctt ttctctcggg gtggaaagta
     2701 atactcgaga aaccggtcac gaaccggtat tccggcaagt tccgcaagcc ccgcgtaaaa
     2761 atccgcggcg tgttgccggg aataaccttc aggaagcgga taacggatat tatgaaaaat
     2821 accgcggcgt ttgtaggcaa accccgcgcg tttcggaatc cccagaaaga aaaacgccca
     2881 gaaggcgttc tcgggacgga gcgaaaaatc gaggagaaga tcataccgtc tggcacgaag
     2941 ttttagcccc aaatccagaa gctcacggaa attttcaaaa cgggtctggc ggcgcagttt
     3001 gtccttatca aggacaaaaa tttcgtcgac gtgcggattg tgctccacca cttctctcgt
     3061 ccgggacccc agaagaagat ccaccgttcc taccgtgggt agcaaacgca atgccctgag
     3121 aacgggcgtt acaaaaagca aatccccgat cccataagga tggacaatta aaacacttcg
     3181 gatatttttc attcagcctt accctttcac gaatccgctc aaaccccgtg tgtcaacaag
     3241 gccctcaccg tcaaaccgag cgggtcaaga gatctcgtgc cgccgcgtaa accgcgtcaa
     3301 cggtaatttc atcaatgtaa gtgaactttc tttcgctaca aggaacctcg gaaatatctc
     3361 tagccacaac gtggcagttc cggcccagag gccgccatcg atttggccca agcccgggct
     3421 cataacgccc gaaaatagac acgaccggac aacccaccgc ggacgcaatg tgtaccggtc
     3481 cggaatcgtt agagaccagg atggcggatt tctccaagag agcggccaac atcccgagag
     3541 tcagtctgcc cccaagatcc atgatcggta cattgctttt ttctctgacg ctttccacga
     3601 tcgctctgtc cttggcagag ccaattgcca taataattcc atccatgtcg tgatgaattc
     3661 gcgtggcaag ctctgaaaat ttcccggcag gccacatctt cgtcacgtcg ctggcggaag
     3721 gactcaggat gacccatggt ttcccttgtg ggattttgag atgaaacatc agatggtcaa
     3781 aggatcgttt cgcgcgatcc gtcacgggga aaaagctctc aagaacctca ggtctggtaa
     3841 tcccaagcgg ggccagcaga tcaaataggt attcagcttc ctgtttctct ccgaattttt
     3901 tatcatactc tttaacgtga gttagcgccc atggcgcgcg gcgatcataa ccgatacgcg
     3961 ccggtatgcc cgctaaccat gccacaagat gcatccggtt cgtaccgtga caatggatga
     4021 caagatcaaa tttctttttg cggattcgcc tcgcaaatcc aagctggccc caaatgtttt
     4081 tttcaagacc ttgtttgtca taaaggatta cttcatcaag aaagggattt ccttcaacca
     4141 cttcccgatg ctcaataaaa acaagcgccg cgatccaggc ttttggaaat tgatcccgaa
     4201 gcgccttaaa aacgggagtc gtaagaacaa gatccccgat acggtcggtc cttgtcacaa
     4261 ggatacgggg agaatcggga agcgtgatat tcttcatgat ggccgggcgg cgggagtatc
     4321 ccggttcaaa agctcattgc acaaacaaag tactttttcg ggggtcaggc cgtggaaaca
     4381 actgcgttcg gcataaggca aacgatggga attacagtga cactgtgtgg ggttttcccg
     4441 cgcgatcctg aaactcggat gaacatgccc gtatttttca tgatccgtcg ggccaaaaat
     4501 tccgaccgtg ggagttttta tttcgaaccc aagatgcatg atcgcgctgt cattggagat
     4561 taccagtgac gctttcgaga caagaaaggc cgtttccgac agtgacgttt cacccgacag
     4621 aattttgacg gaagccggca tccctctggc aacgacttca gcaatcggac gctcgtccct
     4681 tgatcccacg agaaaaacgt ccttgcgggt tcgcaacaca aggttccgca ccacctcccg
     4741 aaacaactca ggcggccacc gcttgcgttc actcgcagcc ccgggcgcaa caaggatcca
     4801 tcctgacttt ccctgaattc cgatggatcc cagcttagaa aaaactgaaa gttcatccgc
     4861 tgaactgaaa aattgaaaat tgggcaaccg ggtatttccc gaaaaaaaag tttcctcgcc
     4921 tttcgtcccg ttccttatcc ccgccattct cagcacttca agatgccgct cccgcatgtt
     4981 gatctttgtg taagcgcgaa aaagcgggct ccttttacgg gcaaaaacaa ggaacgggat
     5041 cgccgtatta cgaagatcca ctaccatatc gtaacgatct ttccaaagct cacgcaaaaa
     5101 tgccacctta ccgggaaggg tcgctttttt gtcgtagaca ataatccgcg aaatatcgcc
     5161 gcttttctca agcacaggct tcgctttggg cccaaccacc accgtcattt ttgcgtcggg
     5221 aaatttcaag cgaagcgccg ccatcaccgg agtcgtcaat atcacatcgc cgatattact
     5281 caatgtcaca acgaggatac tttcgggatt cttcataaat tttgtcccgg ttgaggtgga
     5341 aacgcaccca gttgttccac cgcttcaaac actgcttcgg gggttatttt tgaaagccag
     5401 ccttcttcag gaagctcttt cccaaaaaac ggtatggaat acccggaggg gacatactgc
     5461 aaaactctat tctccccaat cccgcggggc ccggtcaagt ggggatccgt gggaccaaaa
     5521 agggctacga cacgcgcgcc agcgcctgat gccatatgca tcggcccgga gtcacccgtg
     5581 accaggcaca tggcattttt ataaatccag gcggaaatcc gaagggccgt ttttccgaca
     5641 agatccacga ctgcgggatc gagctccccg tttcccgtca agcgtccgat ttcggcccga
     5701 aaattttcaa atatagccct atcccccgcc gatcccgtaa ccacgatggg aacaccccac
     5761 ttcccgaaaa ttttaaaggc aagcttcgcg aagtgagaca cgggccaccg cttgggttcc
     5821 caattcgccc ccagatggaa acagacgtac ctcgcccctt ccgcgagacc ttcctgtctc
     5881 agaattttta gagcctgaac ttcatctcct tcccgaaaat agaacctgta tttcgggctg
     5941 acagggacct ggtaaccagc gcccttcata agctccatga aataatcgag ttgatgaagt
     6001 ttctccgccg gttccttgag cgattccgcc aaaaaaaact ttctccctcg cccaaaccca
     6061 attcgttttt tgacacccgc gagccaaaga aaagcggcgc gggaacggga acggtgaaaa
     6121 aggtatcctt cctgccaggg acccagtttt ctcagatgaa aaacaaaccc aattcggtga
     6181 aaccagaacc ggtgggtgga tttttcatca aacgcgataa accccgaaac tgccggattc
     6241 gcctccagaa tttcccggca ccgttccgga gctacgcaaa aaatttccgc ggaaggatac
     6301 ttttgtttga tcagatcaag cgccggcatc tgaaacaaaa ggtcacccag ccagtttttc
     6361 attacaacta gtattttcat aaattcagcc gatacccagc ggagagtttc aacacacttt
     6421 taaaaatcaa gaccatccaa tccctctctt gatttcactt tttatttcaa aatctcacaa
     6481 atccagaaat ctccgggccg cgtcaaaaac ctcatccacc gtaacatggt tcagacagcc
     6541 catcatttct ttttccttgc accttccctg ataacacggt tgacaggata aatttttcgt
     6601 gaaaatcgcg atttttctgg atggcggtac gtggcgtttt ggatccgtcg ggccaaaaat
     6661 cgcggccgtc ggaacatgca tggccgccgc gacatgaagt aacgccgtgt ctccggaaac
     6721 cacgaaatga agtttttgaa agaaagcggg gagatcttca agggtcgtct ttccaatcag
     6781 attcacaaca cgatccggat caaaatcacg aaagacatcc gccaatcctt cttctccggg
     6841 cgagcccagc agaagaatcc tcgcgtcata gtggtcaaaa attttttcag cgagcttctt
     6901 aaaaaactgc ggatcccatc gcttggtcgg ccatttcgga ctcgatccca tgacaaatcc
     6961 gatcaccctg gtatccgcct tgagatccag ggcaagaagc gtttgctccg ctttttcaat
     7021 ccgctctttc tttggccaca gttcgagttc atcctcaaag tcctggacgc ccgctttagc
     7081 aagcacccgg aactgatgac ggaccggagt gtcggcgagc ccaaatgtct tgtcggaccg
     7141 attaagaagt cttccccaga accctctcgc aaatccgcat cgctggacca cccctcccag
     7201 ataagctagg agatgtgtgt acttgttatt gtgaaggtcc actgacatat caaaattctc
     7261 ctgccggatt cgccgcgcgg tcttgagtaa gtagagcgga ttcccgagtt tttttctgtc
     7321 gaccaaaatg agatcgtcca ggtaaggaca attcgagatg atgggcgcaa gtttcttatc
     7381 caccagaatc gaaatcgaag aatccgggta tcggtttttg agcattctca aactcggcac
     7441 caccaaaacc agatccccca tggctccaag cttgatcaca agtatctttt ttttcttccg
     7501 tacctccaga tacacttcca gcgttttttt aaccatctgt tcaacattga atttttcttc
     7561 gatctttttc cgaagcgcga tcgcgcattc cttcgcccac gggcggtccg taagcatcgc
     7621 gtgcatcgcc tccgccattg ccgggacatt cttcggaggg aacagataac catgccggcc
     7681 atgatcaata atgtcgggga caccccccag ccacgtcgcg gccacgggga ttccgaccgc
     7741 cccggcctca ataatcactc tcccaaacgg ctccggcaag gtggaagaaa gaacaagaag
     7801 atccgactcg gcgagaagcc ccggaatatc gcgcgacggc ccaagcacct tcacgcaagt
     7861 atcgagccca aattgtttta tcgtttgatg gatcagtttc gtataccggg tcctgccccg
     7921 attttccgcg ccgacgatta aagcttcaaa gttgggaaac cggcttctca aaaggtgcaa
     7981 cgccttaaga aattccactt gtcctttgtt cggcgaaaaa cgtccgacgt gaataatccg
     8041 gaaccgttta ggtttttctt caaaccgttt cagggaaaaa acaaactggg acagatcaac
     8101 ccctcgggga atcagccgga tccggtcttg aggcactcga aactcgtcaa tcatccttcg
     8161 tccaatcgcg ttggagatga caatcacacg ctttccgtac cccatcacaa agctcatcgg
     8221 atgaacggag taatgcccat gacaagtggt aataaaaggg attcctgttt tccgagaggc
     8281 cagccatccg atccaagcgg gaacccggct tcgggcatga acaatatcga tccgctcccg
     8341 ctcaatgatt tttacaagct gatcaaccag caataaagag acgatcgact tttgatgaac
     8401 cggaagccca taatgaggga cccccatgcg ttggagttcg gcaaccaggg tcccgccgga
     8461 agaaacaacg acggtctcat gcccttcctc ttttaaagaa cgggccatat ccaccacccc
     8521 acgttccaca ccaccgatct caagcgccgg taacaattga agtattttca taccacggtc
     8581 tctgagtcca tattgtctgt ttctgggttc atttacaaaa aagccaccgt ccctcgcggt
     8641 attagactaa aaatccggga acgcctcaac gcaaaatcat aaagataagc acatgacaca
     8701 acccgcttga ttccgcacgc gttcttttgt tctcatttta taacagagat ccgagtttct
     8761 gcaaaagtgc gtttcgttca cgatccagca tgggaggcac ttctttcttc ataagttcag
     8821 cgacggttct atccagatct tcaaggccgc tcatcgtgac aaggccccgc tgaatcagga
     8881 tctgatgaaa ccggtagtgt ttggccggaa gctcaccttc tccaagatgc aaaacgatca
     8941 ctttttttcc cgcacccacc gcttcggaaa tcatcgccaa gctgtcttcc gtcacaatca
     9001 caaggtccgc gagttgcatc atcccccccg cggcaaaagg aggattgtcc tcattcgcga
     9061 tcacgaggta acggcaggcg ggatcctggg caagcacact tttaagaaac tgctccgtca
     9121 ttcgactcgt tcgccgggaa gtcgtcagca tgtactcggc agatttccgg caaattcgcc
     9181 tgaggatcat ggctgtcttt tccacgtccg gaaccgtaat atcaaatccg cgggttttgc
     9241 caccgaggaa caccgcaatc cttgcctttc ccgtttcccc aagctgggtc ctgaggatcg
     9301 ccgtatctcc cgagcggtca ggaggccggt atccgctcgg cataatgacg caccgcgtgg
     9361 aattcttggg aaggatcccg tcatcgtgcg cgggaaccac ggccaaatca tagttcatca
     9421 aggaatatgg aaacgggggt ttcataatga caatggtttt tgctccggtc tccctcgcga
     9481 gaatttgctg aacgggagcc aaaccggacc ccgccgcaat gataaaatcg gcggtcgcct
     9541 cttcaaccgc ctggtgacat tgcggtttta aaaatatccg gagccattca attcgcccct
     9601 gaatccacgg cttcagaaat ggcgccatca gaaagagaag agcgcgatgg agggaagaac
     9661 ggtattcaat atgaattttt tcagtttgaa attcgagccc cgccctgccg tgaaattccg
     9721 tgagagagga catcatctcc gcgacaacat ccgactgctt aaaatgcccg gcctttccat
     9781 ccgaaagaat cacgattttt cgggaccagc tatatttcca ccttttgttc tcccagagcc
     9841 actgtcccgg gtattggcta atgaaacgct ccagagaatc aaggtactgg cgcgccagtg
     9901 atctcactct ggtttcagta tccgcggaag ggtcatcttg aagtgtttcc tcgacaaaaa
     9961 tttcatggcg cgaccgatcg attcgaacca tatacgaagg catcaaaagt gagtcggtcc
    10021 ggatcgcaag ctcaaaagcg ccggtcggaa ccgacgtctt cttcccgaaa aaaggaagaa
    10081 ttatgccttc cgtttttccg gcagactggt ccccgagaac gccgatcatt tttttctccc
    10141 gaagcgcctt gattaaggcg cgaactccga ccccgcggcc gacggccacc gacccatgcg
    10201 tctcacgaag ttggttaaga agctcgttca ccttcggaaa tttctggtca cgggcaagca
    10261 cataaatggg cgtcccgcgt aaaccggcca caacctggag aagctcccag ttaccgaagt
    10321 gtccggtgag aagtacgccg ccccgtcctg attttacaag gtcctcaaag cgctccaggt
    10381 ggtgaatttt gatatgatct tcaatgtgcc tgggagtcag cttcgggaaa ctcatcacct
    10441 ccacgatgtt ctgtccgata tgcccatagt ggtcccgaac cgttttccat cgctcccggg
    10501 gagataatcg ggttcccagg gcgtttttga gattaacgta agcgacccgc cgttttttcg
    10561 aaaaataaaa aacagttttc ccgatcgcgt ccccgatccc caggcttgtt tcgatgggaa
    10621 tttttccaaa aagaaaaatc agggatcgcg cgaccccgta agcgatccaa tcgagaattt
    10681 tttccattaa cgtttaatcc ccaaaagacg caacaaccga tccgtcaaaa tttcctcccc
    10741 ggaacgaatt cgaagccttg ccgcgagcac gaggggatta aggacttgcg tgattaaatc
    10801 ccgggaacgg taaaaatcct tttccgtcgt gataatctcg tcgacgtctg ccgaacggct
    10861 gatttcctta atctccttca attccgattc cttatattca tggtgatcca gaaactcaaa
    10921 atttcgagcg gtcttgattg aagcgtgttg cagtaccagc tgaaacgagc gcggggtgcc
    10981 tactgccgca aacgtggcga cttttttatg tcgcattttt tcaagtgaaa ccctcgaacg
    11041 attcttggcg cgataaagaa aaagaggttc catgtaagct tccacaaaat gcgctcgagg
    11101 cgaaaggctc tggatctgct cccgaagact cttgagctgt tcggggggca cgagattaac
    11161 gtgggtcaga acgaccaccg tcgccctttt taaaacgctc atgggctcac gaagaatacc
    11221 gcgcgggatg aggtgctggt tgccaaacgg gttaagagag ttaacgacaa taatctccac
    11281 gtcccgctcg accgccacct gctgaagccc atcatccaga atcgcgacgt ccacccgttt
    11341 ttccttcaca atacccaggg ccgtcttcac tcgatcacgg ccgacaccaa cgaccaccgt
    11401 tggcatgtga tgccgaaact cttcaacctc gtcgtgatta tatccacgcg tgagaatcag
    11461 cggggttttt cggaacgcgg aaatttttcg ggcaaggaac tccacaagcg gcgtttttcc
    11521 tgttccaccc caggtgagat ttccgacact aatgacgggg aacggcaatt tggaacgttt
    11581 cagatatttt ttttcatgcg cccatcgcaa ccccctgacc cccaccccat aaacgaacga
    11641 cacgacacta agcaggggac acaagcaaaa agaggccgcg tcacggcatt tcccctctgc
    11701 caccagccga aaatagttct tgagattctc catctgtttt cttcctttct gatgccatcc
    11761 gggacgcgag gaacatcaac acccattccg cctggcattt gctcgctccc ttaagacgat
    11821 tgacgatctg attcgctttc acacccgcct cacggcgcag ggccggatcc gatagaaact
    11881 tctctgacat agcctttagg tctcccggtt cccgtacaag aaaagccccg ccgccctgac
    11941 cgagctcgcg gtagatcatg ttgaaattaa agacatgcgg tccgtgcatc acggcacggg
    12001 caaagcgtgc cgcttccacc ggattttgtc cgccatgcgg aaccaggctt ccgcccatga
    12061 acacaagatc cgcaatttga tacagctctt ttaaaatgcc aagttggtcc agcaccaaaa
    12121 catcaaaact tccgggcgcg ggttttcggg atgccaaaac aattcgacac gaacccgtgg
    12181 aaagatcctt caacagcctt ccggaacgtt cgatgtggcg gggagccaca atcatcttga
    12241 gggcgggaaa tctctttcga agttctaaaa aaacatccgc aaggattttt tcttctcccg
    12301 ggtgcgtact gccagctatc aaaacaagat cttccccgtt taccccaaac cgttccctga
    12361 tcaaatcagg cgacaagctc ttgccccgtt ccagctcaac ttgatcaaac ttcatattcc
    12421 ccatctccct gaccgatttt tcaaaaaccc ccaattcgcg aaatcgccgg gcgtcttccc
    12481 gtgtctgggc gagcacaaaa tctatcttct tccagagccc agacaaaagg aaacccgcgg
    12541 cacgatatcg tttaaacgac cgttccgaca acctcgcatt tacaacgccc acgggaatat
    12601 tccgccgctt cacctcaatc aggagattcg gccaaatctc agtttcggcg aggacaaccg
    12661 ccaccggctg aatctcatcc aggaaacgcc ggaccacaaa cgagatatca aacggaaagt
    12721 aggccacccg gacccgggga ccttccattt ttttcgcgat tcgctggccc gtgggagtca
    12781 ccgtcgtgag aaaaacatca agatcccggt cccgttccag caattcttgg acaaattttt
    12841 cgacggcatt aacttctccg acactaaccg cgtgaagcca caccgttttt ccggaatccg
    12901 gacttttttt ccggtttcct gaaagattcc cccaccgttc ccgccaaagc cgcttggggt
    12961 cttccgcctg tctgatcttc atcagaaaaa ccggaaaata aaacattcca aataccaaga
    13021 aagccaggtt atataaaaat atcatcgcca tgatgaatac ttcccgcctc cgcaacacgg
    13081 cccggatcgc tcaagctctt ggatgtgccg tcgcataggc atccttaaga cgctgagtag
    13141 tcacgtgcgt gtaaatctgg gtcgtcgaaa gattcgcgtg gcccaaaagt tcctgcacgc
    13201 tcctgagatc cgctccccga tccagcatgt gagtggcaaa agagtgcctc agcatatggg
    13261 gcgaaacttc tttcccaaga cccactcgtt tcacgtattt caaaatcatt cgccgaatac
    13321 tacgatccgt caaccggccg cccagtcggc tgacaaacag cggttttttg atcgcgtgtt
    13381 gcggttcttt ggggggtttt tcatccaggt accgcttaag acaggaaatc gcggtttgtc
    13441 ccaccggtac gatccgctcc tttttccctt tcccccgaac cctcacccaa cccgcaaaaa
    13501 gatcgcaatc atcatgattc agccccacaa gctcgctcac gcgaaaaccc gagctgtaga
    13561 gcatttcgag aatggccttg tcccgttttt cttcccagct caccttggcc ggcgcttcca
    13621 acagtttggt gatctcatcc ggattaagaa actcgggaag tcttttttct tttttgggag
    13681 tggcaatgcc cgccgcggga ttttgcgtca aaacgttttc acgagtcaaa tacttgaaaa
    13741 aagaacgcag gcaggcgagt tttctcgcga tggaactttt actgtattcc cgtgttttca
    13801 gaaaagccag aaaggagcga atgtccaggt acgttatttc cgcgagatct ttttcagaaa
    13861 gaaacttcac gaattcgcga aggtcaatgg cataattttt taccgtgtga atggaagccc
    13921 ctttttccgt ggtaagaaac cctagaaaat cctcaacgcg ctgatcggtc gccataattt
    13981 caggtgggat gagaggtcgg atcggaggag ggctcggagt tttcgccttt tttgggaagc
    14041 tgttgcaccg tataagtgca ttgaggatag ttggaacaac catagaatgc gcgtctcccc
    14101 ttggccttgc gcttcacgag ttctccgcca caccccggct cggggcagaa aaaacctgtc
    14161 gggatggacc tcgtatattt acaatccggg aacccggaac acgcaagaaa tttcccaaat
    14221 cgtccgaact tgatcaaaag ttgtttgccg cagatatcgc atttaaattc cgtcgggaca
    14281 ggttcccgct taaaattttt catctcttca cgggccttga tcaaaagctc attaaagggc
    14341 ccgtaaaaat cataaagcac ctttttccac tctctcgtcc cctcttccac ctcatccagt
    14401 ccctcttcca tcagagccgt aaactcagcc tcaagtacct tggggaaata ttccaccaga
    14461 aggtcggtga tcatttctcc cagttccgtc gggataagcg ccccgccctt cttttcgcag
    14521 taatcccggt tcagcaatgt tgaaatcgtc ggggcataag tactcgggcg cccgatgcct
    14581 ttttcttcaa gaagcttcac gagacttgcg tcattg
//
LOCUS       DAOSIO010000015        14385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_4302_length_14385_cov_4.438730, whole genome shotgun sequence.
ACCESSION   DAOSIO010000015 DAOSIO010000000
VERSION     DAOSIO010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 14385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14385
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4302_length_14385_cov_4.438730"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PK590_01285"
     CDS             <1..509
                     /locus_tag="PK590_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23261.1"
                     /translation="FKIAGLAFLALNIASLGVVAYAEDDAGSTPAGWSQGKKTGWDSG
                     SHPPGWAKREARKGEIKLKEATGEAGKATRGAKVKNAQEKTAKEAKRAEEKARKDAEK
                     LKAKSEKEAKKAQKKAEKEAKKLQKENDEARKRAEKQVRKQERESRKKAEELTGKSSR
                     QAKGFAGF"
     gene            585..1361
                     /locus_tag="PK590_01290"
     CDS             585..1361
                     /locus_tag="PK590_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG23262.1"
                     /translation="MKKNKKKAIIIGTGSGIGRALAFKLSGEGYFVGLTGRDPEKLKA
                     LQAELPGESVARPLDLRDPREAIHSLGNLIMYMQGMDLMIINAGVLFRNEELVWAKEE
                     ETVKVNVAGFAAMANSAVHYFQKQKSGTLVGISSVAGHRGSGRSPAYNASKAFMINYM
                     EGLRQKLFGTAIRVIDVRPGFVDTDMIRGNRGLFGVISPEQAADQIFRAIRKGKKKVY
                     VPGWWGPLTYCFRKLPEWIYHSGYKKYLSWDTRVRARRSQ"
     gene            1371..1910
                     /locus_tag="PK590_01295"
     CDS             1371..1910
                     /locus_tag="PK590_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOG23263.1"
                     /translation="MRKLTHVELLERQQKRKRVPKLPFCVVLNHIRSLHNVGSIFRTA
                     DGAGVEKLYLCGITGHPPDSKITKTALSAEKEVPWEYCQDAVEVLRRKKQEGYEIVLL
                     EQLAQSIPYETWTPQGPVCLVIGNEIEGVSDELLALCDRTIEIDMAGVKNSLNVSVAF
                     GIAAYHIRNQIRERARADS"
     gene            1916..2188
                     /locus_tag="PK590_01300"
     CDS             1916..2188
                     /locus_tag="PK590_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOG23264.1"
                     /translation="MTILFSGTVQGVGFRWTAESLARRFPVTGYVRNLEDGRVEVVAE
                     GDEPVLVEFLSAIRHSGMKNYIRGVETCWSEAQSCFKGFRISYEKK"
     gene            2256..2975
                     /locus_tag="PK590_01305"
     CDS             2256..2975
                     /locus_tag="PK590_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOG23265.1"
                     /translation="MRWAVIADIHGNLEALERVEAYLKEHQLERVMCLGDSISYGANP
                     NECLAWVFRHCECHVLGNHEAALVHESLRWDFGGWARVALDWTAERMDPGLMARLREL
                     SYSQIHELMTMAHGTIDRPEAFEYLTEGEAVIKSFSALKTFAGFVAHTHIPGWFSEKK
                     PQGGGYLDEGKIRLAKEDRYLLNPGSVGQPRDYDPRLSFAVFDSEELTFEIVRLPYDN
                     EKAAKKIRSEGLPRTLADRLL"
     gene            2998..5040
                     /gene="ligA"
                     /locus_tag="PK590_01310"
     CDS             2998..5040
                     /gene="ligA"
                     /locus_tag="PK590_01310"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOG23266.1"
                     /translation="MDATALKKKIESLRREIERHNRLYYDDARPEVSDFEYDRMMREL
                     IDLEKKHPEFLTPDSPSRRVGGAPLKEFKTVRHSVPMLSLDNTYSREELADFDERVAK
                     VLGAGKYSYFVEEKVDGVSIALVYEKGFLKLGATRGDGKQGDDITENIRTIQSIPLRI
                     PVPGSGFKGPPPAVLEVRGEAYIPTRQFEKINEEKERMGEELFANPRNACAGSLKLLD
                     PALVAARKLDAFMHGFVRCEGGDHPQSQSQAMRLLRSLGFKTVPDSEKCATLDEVYQK
                     IDSIAAKRDQLPYETDGVVIKVDALEGQRILGMTSKSPRWMIAYKYPAAQAETVLEDI
                     KIQVGRTGVLTPVAILKPVRLAGTMVSRASLHNQDEIKRLDVRIGDHVFIEKSGEIIP
                     QVISVNTEKRAGDLPKFVFPKQCPVCGESVLKHGGEVAVRCLNPLCPAQLKGAIRHFA
                     GREAMDIENLGVSIIEQLVDKGLVKSVADLYTLRQKDLEALERMGAKSAKNLVEGIEQ
                     SKKASLARLIYALGIPDVGVHTAYILAGQLGSMARLSESSAKDLEGIREIGPVTAQSI
                     LDFFARSSTKKLLRRLEESGIAMDRAEQTAGESPLQGKTVVLTGTLERLDRAEAEALL
                     RKFGAHPSGSVSKKTDVVVAGPGAGSKLDKARALGIRIWDEKQFLKELGMEGGRDA"
     gene            5033..5161
                     /locus_tag="PK590_01315"
     CDS             5033..5161
                     /locus_tag="PK590_01315"
                     /inference="COORDINATES: protein motif:HMM:NF023880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose acetyltransferase domain-containing
                     protein"
                     /protein_id="HOG23267.1"
                     /translation="MREYGKMIAGQLYNTLDPELTRMRRAALELCDTFNRSTPRRA"
     gene            5303..6226
                     /gene="xerD"
                     /locus_tag="PK590_01320"
     CDS             5303..6226
                     /gene="xerD"
                     /locus_tag="PK590_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOG23268.1"
                     /translation="MNKSAKTPDKSGNKNQFLFDEFLNYLSVEKGLAKNTLEAYRRDL
                     VRYDEFIKKEKISDWSDIRRAHILKFLAAEGRRGLESTSVARALVSIKLFHRYLAKER
                     YAREDVTSVLESPKLWKKLPYFLTPQETEAILNAPDPKTPMGIRDRALLECLYATGMR
                     VSELTGLTIENVYLDNHFIKCRGKGSKERIVPIGSVAVSACQTYLEKVRRKIKAPTSH
                     FFLGRNGKGLTRQSVWKMIKKYAIRAGITKPIKPHTFRHSFATHLLERGADLRVVQEL
                     LGHSDISTTQIYTHVSGNRLKSVHAQFHPRG"
     gene            6326..6607
                     /locus_tag="PK590_01325"
     CDS             6326..6607
                     /locus_tag="PK590_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23269.1"
                     /translation="MRKVSKMIGLGVIVAWALFPLPSVAVAQSDVQSADSSKTRTSDD
                     YSVIQQDLQQSKLSDALKRIQMLEQENRFLAKRVELLERNVNDIKNDRY"
     gene            6973..7047
                     /locus_tag="PK590_01330"
     tRNA            6973..7047
                     /locus_tag="PK590_01330"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7009..7011,aa:Glu,seq:ctc)
     gene            7131..8441
                     /gene="purB"
                     /locus_tag="PK590_01335"
     CDS             7131..8441
                     /gene="purB"
                     /locus_tag="PK590_01335"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOG23270.1"
                     /translation="MIPRYTRPEMAKVWTEENKLQKWLDVELVALDGLARYGYIPRDI
                     PSKVRAKAKFNIQRVKEIEKVTNHDVIAFLTNVAEYVGPAGRYVHFGLTSSDILDTGL
                     ALQLVDASQILKREIQKLIHILSKQAKKHAGTLMVGRSHGVHAEPTTFGLKMALFYAE
                     FQRNLERFERATENIRFGKISGAVGTFANVDPKVENYVCRKLKLAPAPVSTQILQRDR
                     HAEYMTVIAVIGGSLEKLATEIRGLQKTECYEVQEYFAKGQKGSSAMPHKRNPITCER
                     IAGLARILRGNALAALENIALWHERDITHSSVERVIFPDSTILLDYMLGLMQDVIENL
                     QVNKERMLSNMESSRGMVFSQGLLLKLVEKGLTREDAYKLVQNAAKRIWEDVNANLRE
                     VVLNDKEIRKHLSASEIQSVFSYKRHTRNVSAILKRAGVLKTRK"
     gene            8481..9401
                     /locus_tag="PK590_01340"
     CDS             8481..9401
                     /locus_tag="PK590_01340"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOG23271.1"
                     /translation="MVRDLEGNAILETHLPLPRIAVGKVRDIYGVGSDKLLIITTDRL
                     SAFDVVMPTPVPLKGKVLNQITLFWQNYFRDVIPQHIITADVDEMSLPDEVIKNFRHE
                     IEGRSMLVRKAQPLEIECVVRGYITGSGWKDYQKTGMVCGHKLLPGMKQCEKFPEPLF
                     TPSTKSKAGHDVNIDFGEAIRIVGKDVAMQVRDLTVALYKKGQEYAASKGIIIADTKF
                     EFGIRDGEIILIDEILTPDSSRFWPADQYETGHDQPSFDKQIVRNYLETLAWDKRPPG
                     PELPPDIIEKTTAAYCEIFKRLTGKDIRQV"
     gene            9535..9690
                     /locus_tag="PK590_01345"
     CDS             9535..9690
                     /locus_tag="PK590_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23272.1"
                     /translation="MKFKARIYTSLKKSVLNPQWQTTLRVLAVFDKTKLVSCHDLGAI
                     SFAMERR"
     gene            9690..11123
                     /gene="purF"
                     /locus_tag="PK590_01350"
     CDS             9690..11123
                     /gene="purF"
                     /locus_tag="PK590_01350"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018123538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOG23273.1"
                     /translation="MGGENIKHHCGIFGIYNHPQAARLTQLGLFSLQHRGEEAAGICA
                     SDGEDLHLVKGWGLVGDVFDETSFSSLRGNHAIGHVCYSMAGASTPLNAQPYKVDYSR
                     GQIAIAHNGNLVNAQILRAELEAYGSIFGSTMDSEILIHLIAKPTYRTHEEGIVESVK
                     RVAGAYSLVILTENQLYGIRDPHGFRPLCLGKLGDSYVLASETCAFDLIEAKFVREIE
                     PGEVVVIDRKGVRSHFPQKGTREAGASPAHCIFEHVYFARPDSRVFHENVGLIREKFG
                     RQLAKEHPADADIVVPVPDSGTFAAMGFSKESGIPMAFGFIRNHYIGRTFISPGASNR
                     SFKVKIKLNLIREIVEGKRVVVVDDSIVRGNTAKSRVKLLRAAGAKEIHMRISCPPHV
                     SPCYYGIDFPSKKELLAANYTVAEIKKFLGVESLGYLSLEGMLGSVTRPRKDFCVACF
                     TGKYPTPIYGGRGKNKLEQKAVKCCGH"
     gene            11137..12171
                     /gene="purM"
                     /locus_tag="PK590_01355"
     CDS             11137..12171
                     /gene="purM"
                     /locus_tag="PK590_01355"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOG23274.1"
                     /translation="MKKTDKKEWTYEKAGVPHLKGDETYNRKIVSLIRSTHVKGVMGS
                     ALGFASLFDLKKSGFKDPLLVASTDGVGTKLELARLRSRHDTIGIDLVAMCVNDLICC
                     GAKPLFFLDYYATGKFQPSVVGEVLRGVTDGCRQAGCALVGGETAVMPGFYGDKAPGQ
                     AAQYDMAGFAVGAVDRKKVISGGGIRQGNIVLGIQSSGFHSNGYSLLRKIFSDKELKG
                     PIGKELLTPTFIYVRPILSLIKRVAVKGIVNITGGAFYDNLPRVIPQGLTAVIDSRAW
                     DIPPVFRKAQKAGNISDFNMFKTFNMGIGMVVILEPGQVQRARSILKTFRLRSWPIGK
                     IIKGQEVKVI"
     gene            12168..13448
                     /gene="purD"
                     /locus_tag="PK590_01360"
     CDS             12168..13448
                     /gene="purD"
                     /locus_tag="PK590_01360"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012938624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOG23275.1"
                     /translation="MRVLLVGSGGREHALAWKIAQSPKLTKLFCAPGNAGIAEHAECV
                     DISSDDIEGLLKFAIEQAIDLTVVGPEAPLVAGLYDQFNARGLKVFGPSAKMALLEGS
                     KSFSKDVMSRFGVPTAAYEVFTNAKEAMQYTLETEMPIVIKADGLAAGKGVVICDTSE
                     QAVSTLSKMMEQKVFGPAGNKVVIEKKIEGEELSILVLTDGETILPLASARDHKRAYG
                     HDRGPNTGGMGAYSPSAQFDESMIGSIIDQTIRPVIDGLRKEGSPYRGVLYAGLMMTA
                     DGPYVLEYNCRFGDPETEVILPRLKTDLLPVLAEIADGKLCQKSLEWYEKACMTVVMA
                     SGGYPGSYQKGLSIHGIPAAKAIRDVVVFHAGTALNEKKEVVTSGGRVLAVTALGDNL
                     RAAYDLAYQAIQHIHFEGGFFRRDIGRKAMEVLK"
     gene            13445..13954
                     /gene="purE"
                     /locus_tag="PK590_01365"
     CDS             13445..13954
                     /gene="purE"
                     /locus_tag="PK590_01365"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOG23276.1"
                     /translation="MTQALVSIIMGSDSDLDVMQECAEVLKEFNVPFSMQVMSAHRTP
                     DIVAKFASLAAKNGIRVIIAGAGGAAHLAGAVAAHTTLPVIGVPLAATDLSGMDALLA
                     TVQMPAGIPVATVAVGKPGAKNAALLAVQILSLTNSELAKVLESYKIKLAESCQKKNE
                     KLQEKLKKT"
     gene            13983..>14385
                     /locus_tag="PK590_01370"
     CDS             13983..>14385
                     /locus_tag="PK590_01370"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOG23277.1"
                     /translation="MSVPEIIKLDPRDPDCVQLKRVVDASREGEVVGFPTETVYGIGG
                     LMSVPGVEKRLQEIKGRREGKPFAFHIGDWDMIDRLGVVQTPMVRFLMRSFWPGPITL
                     VVKNTAGETIGVRFPRNKVARLLIRESGEPFI"
BASE COUNT         3506 a         3279 c         4284 g         3316 t
ORIGIN      
        1 ggtttaagat cgcggggctg gcgtttcttg ccctgaatat tgcgtcactg ggtgttgtgg
       61 cttacgcgga agatgacgct ggatcgacgc ccgcgggatg gagccaggga aagaaaacag
      121 ggtgggatag tggcagtcat cctcccggct gggcgaaacg ggaagcccga aaaggtgaga
      181 tcaagttaaa ggaagcgacg ggggaagccg ggaaggcgac ccggggagca aaggtgaaaa
      241 atgcccagga aaaaaccgcg aaggaagcga agcgggctga ggaaaaagcc aggaaggacg
      301 cggaaaagct taaagccaag tctgaaaaag aggcgaagaa ggcccaaaag aaggcggaga
      361 aggaagccaa aaaactgcag aaagaaaacg acgaggcaag gaaacgggct gaaaaacaag
      421 tcagaaaaca ggaacgggaa tcccggaaga aggctgagga attaacggga aaatcttcca
      481 ggcaggcaaa aggttttgcc ggcttttaaa tcggagaaga cgataaaaaa ggcgttacct
      541 gatcttgttc agggaagcgc ctttttttat ccgtcagaca acctatgaaa aaaaataaga
      601 aaaaagcgat catcattgga accggcagtg gaattggccg cgctttggcc ttcaagcttt
      661 ccggggaagg atatttcgtg gggctgacgg gacgggaccc tgaaaagctg aaagccttac
      721 aggccgaact tcccggagaa agcgtggcgc ggcctctgga tcttcgtgat ccacgagagg
      781 ctattcacag cctcgggaat ctcatcatgt acatgcaggg catggatctt atgatcatta
      841 acgcgggggt tttgtttcgc aacgaagagc ttgtctgggc caaggaggaa gaaaccgtta
      901 aggtcaatgt cgcgggattt gccgcgatgg caaattcggc cgtccattat tttcagaaac
      961 aaaaatccgg cacgctggtg gggatttctt ccgtggcggg ccaccgggga agcgggcgtt
     1021 cgccggccta taacgcctcc aaagctttca tgattaatta catggaagga ttgcgccaga
     1081 agcttttcgg gaccgccatt cgggtgattg acgtccgtcc cggttttgtg gacacggaca
     1141 tgatacgcgg taatcgggga ctctttggcg tgatttcgcc ggagcaggcc gcggatcaga
     1201 tcttcagggc gatccggaaa ggcaaaaaaa aggtctatgt ccccggatgg tggggaccgc
     1261 tcacgtattg tttccgcaaa ttgcccgagt ggatttatca ttcaggttac aaaaaatatc
     1321 tgtcctggga cactcgtgtc cgggcccggc gatcgcagta accgcgcccc atgagaaagt
     1381 tgacccacgt tgaacttctt gaacgtcagc aaaaacggaa acgggtcccc aagctccctt
     1441 tttgcgtggt cctgaatcac atacgaagtc ttcacaacgt cgggtcgatt tttcgaacgg
     1501 cggatggagc cggcgtggag aagctgtacc tgtgcggtat taccgggcat cctcccgatt
     1561 ctaaaatcac aaaaaccgcg ttaagcgccg aaaaagaagt gccctgggag tattgccagg
     1621 acgcggtcga agtcctcagg cggaaaaagc aggaagggta cgaaatcgtt cttttggaac
     1681 agctcgccca aagtattccg tatgaaacgt ggactcccca agggcctgtt tgtcttgtga
     1741 tcggtaacga aatcgaaggt gtttcggatg agttgctggc cctgtgtgac cggactatcg
     1801 agattgacat ggcgggagtc aaaaattccc tgaatgtgag tgtggcgttc gggatcgccg
     1861 cctatcatat ccgaaaccag attcgcgaaa gggcgcgggc ggattcatga aaaggatgac
     1921 gattttgttc agcgggacgg tccagggggt gggattccgg tggacggcgg agtctctcgc
     1981 ccggagattt ccggtcacgg ggtacgttcg taatctggaa gatggtcggg tggaagtggt
     2041 cgcggaaggg gacgagccgg ttctggtcga gtttctttcc gccattcgtc attcgggaat
     2101 gaaaaattat attcgcgggg tcgaaacgtg ctggtcggaa gcccagagtt gtttcaaagg
     2161 ttttcgaatt tcctatgaga aaaaataaga ctcgggtcaa gcgtctgtcc ggtgaaggcc
     2221 ggagggggcg ctgagccggg ggcatggagg gcgggatgcg ttgggcggtg attgcggaca
     2281 ttcacgggaa tctggaagcg cttgaacggg tagaggccta tctgaaggag catcaactgg
     2341 agcgggtgat gtgcctcggg gattcgatta gctatggagc aaatccgaat gaatgcctcg
     2401 cgtgggtttt tagacattgc gagtgtcatg ttctcgggaa tcatgaagcg gctctcgtcc
     2461 atgaatcgct ccggtgggat tttggcggtt gggcccgggt cgctctggat tggacggcgg
     2521 agaggatgga tcccgggctc atggcgcgtc tccgggaact ttcctattca caaatccacg
     2581 aattaatgac gatggcgcat ggaacgattg atcggccgga agcttttgag tatctgacgg
     2641 aaggcgaggc cgtcataaaa agcttttcgg cgctcaaaac attcgccgga tttgtcgctc
     2701 acactcatat cccggggtgg ttttccgaaa aaaaacctca aggcggcgga tacctggacg
     2761 aagggaaaat ccgtctcgcg aaagaggatc gttatcttct gaatcccggg agcgttggtc
     2821 aaccgaggga ctatgacccg cggctttctt ttgccgtttt tgattcggaa gaattaacat
     2881 ttgaaattgt ccggctccct tacgataatg aaaaagccgc aaagaaaatc cggtcggaag
     2941 gattgccccg aacattggca gaccgactgc tttaaatctt aattcgggcc cgggatgatg
     3001 gacgccacag cgttgaaaaa aaagatcgaa tcgcttcgcc gtgaaattga aagacataat
     3061 cgtctgtact atgacgacgc gaggcccgag gtttcggatt ttgagtacga ccggatgatg
     3121 cgtgagttga tcgatcttga aaagaaacat cccgaatttc tcactcccga ttctccctcg
     3181 cgccgcgttg gcggggcccc acttaaagag tttaaaacag tccggcattc ggtgccgatg
     3241 ctttccctag ataacaccta ttcccgggaa gaactggcgg attttgacga acgggtcgcg
     3301 aaggtccttg gggccggaaa atactcctat tttgtggaag aaaaggttga cggtgtttcg
     3361 atcgcgcttg tttacgaaaa aggtttcctg aaacttgggg cgacgcgtgg ggacggcaag
     3421 cagggagatg acatcacgga aaatatcagg acaatccagt cgatcccgct caggattccc
     3481 gtgccgggga gtggtttcaa ggggccgcct ccggcggtgc tggaggtcag aggcgaggcc
     3541 tacattccga cgaggcaatt tgaaaagatc aatgaggaaa aggaaagaat gggcgaggaa
     3601 cttttcgcca atccccgtaa tgcctgtgcc ggaagcttga agcttctaga tcccgcgctg
     3661 gtggccgcgc ggaaactgga tgcctttatg cacggtttcg tgcgttgtga agggggagat
     3721 catcctcaaa gccagagcca ggccatgcga cttctgaggt cactgggctt caagactgtt
     3781 cctgattccg agaagtgcgc gacgctggat gaggtttacc aaaagattga ctccatcgcg
     3841 gcgaaacggg accagcttcc ttatgaaacc gacggagtcg ttatcaaggt ggatgcgttg
     3901 gagggccaga ggattcttgg catgacctca aagtcaccgc gctggatgat tgcctataaa
     3961 tatcccgcgg cgcaggccga gaccgtttta gaagatatca aaatccaggt tgggagaacc
     4021 ggcgttctga ctcccgtcgc gatcctcaaa cctgttcgac tggcggggac gatggtttcg
     4081 cgggccagtc ttcataacca ggatgaaatc aagcggctgg atgtccggat cggtgatcac
     4141 gtttttatcg aaaaaagcgg cgagatcatt ccccaggtca tttcggtcaa taccgaaaaa
     4201 cgcgcggggg atttgcccaa gtttgttttt ccgaaacaat gtccggtttg cggtgaatcg
     4261 gtgctcaaac acggtgggga agtagcggtt cgatgcttga atccgctgtg tccggcgcaa
     4321 ttaaagggcg cgatccgtca tttcgcggga cgtgaagcca tggatatcga aaacctcggt
     4381 gtttcgatca ttgagcaact ggtggacaaa ggtttagtca aaagcgtggc cgacctttat
     4441 acgctcaggc aaaaggatct ggaggccctg gaacggatgg gagcaaagtc cgccaaaaat
     4501 ctggtggaag gtattgagca aagtaaaaag gcgtcgctcg cgcggcttat ttacgcgctc
     4561 ggaattcccg atgttggcgt tcatacggcg tacattctgg cgggacagtt ggggagtatg
     4621 gcccggcttt ccgagtcgtc cgcgaaagac cttgaaggaa tacgtgaaat cgggccggtg
     4681 accgcgcaat ccattcttga ttttttcgcg aggagttcca cgaaaaaact tttgcgccgt
     4741 ctcgaagaat cggggatcgc gatggatcgg gcggaacaaa cggccggcga aagtccgttg
     4801 caggggaaga ccgttgtact gaccgggacg cttgagcgtc tggatcgcgc cgaagcggaa
     4861 gcgttgctcc gaaagtttgg agcgcatcct tcggggtcgg tgagcaagaa gacggatgtt
     4921 gtcgtggccg ggcccggcgc cggcagtaaa cttgacaagg cccgagcgct tggaatcagg
     4981 atttgggacg aaaagcagtt tcttaaagag cttgggatgg aaggggggcg tgatgcgtga
     5041 atacgggaag atgattgccg gacaactcta taacacgctg gaccccgaac tgacccggat
     5101 gagacgggcg gcgcttgagc tttgcgacac cttcaaccgt tccactccac ggcgcgcttg
     5161 attcgcttcg ttaaaaaacc ctgccgtccg gactaacgcg agcccgtcac gagcccgtga
     5221 ggtatcgtca attcgggata ggaaatcctg gaataactta accgtctcca gttcggatat
     5281 cgattggccg atacctgagt caatgaacaa atcagcgaaa acacccgaca aatccggcaa
     5341 taaaaatcaa tttttgtttg atgaatttct gaattatctt tcggtcgaaa agggccttgc
     5401 gaagaatacg cttgaggcct accgtcggga tcttgtccgc tacgacgaat ttattaaaaa
     5461 agaaaaaatt tctgactggt cggatatccg tcgggcgcat attctgaaat ttctcgcggc
     5521 ggaaggccgg agggggcttg agtcgacttc ggtcgcgagg gcgcttgttt ccataaaatt
     5581 atttcaccgc tatcttgcga aagaacggta cgcgcgcgaa gacgtcacaa gtgttctgga
     5641 atccccgaaa ctctggaaaa aattgccgta ttttctcacc ccgcaggaga cggaagcgat
     5701 cctgaacgcg ccggatccaa aaacgccgat gggtatacgt gaccgggcat tgctcgaatg
     5761 tctctacgcg acgggcatgc gggtttccga gttgaccggg cttacgatcg aaaacgttta
     5821 tctggataac cacttcatca agtgccgcgg aaagggaagc aaggagcgga ttgtgccgat
     5881 cggaagcgtc gcggtttcag cgtgccagac ttatctcgaa aaagtccgcc gtaagatcaa
     5941 ggctcccacc agtcattttt ttctcgggcg gaacggtaag gggttgaccc gtcagtcggt
     6001 gtggaagatg ataaaaaaat acgcgatccg ggccggcatt accaagccga taaagccgca
     6061 caccttccgg cattcttttg ccacgcatct gcttgagcgg ggcgcggatt tgcgcgtggt
     6121 ccaggagctt ttgggccatt cggatatttc cacaactcaa atttacaccc acgtgtccgg
     6181 aaatcgcctc aagtctgttc acgcgcaatt tcatcctcgg ggataacaga agtccccagg
     6241 gttttgtttc ccttcacgat aatcgttggt ttttcgcctc tttggccttt ggttcgtccc
     6301 ccaggatatg gtatacttcg gccctatgag aaaagtgagc aagatgatag ggctaggggt
     6361 gatagtcgcg tgggcgttgt tcccgttgcc aagtgtggcg gtagcgcagt ctgatgtcca
     6421 gtccgctgat tcgtcaaaga cccggacctc tgacgattat tcggtgatcc agcaggattt
     6481 gcagcagagt aaattgtcgg atgccctcaa gcgaattcag atgctggaac aagagaaccg
     6541 tttcctcgcg aagcgggtcg aattgcttga gagaaacgtc aacgacatca agaatgaccg
     6601 ttactgagta agctcgggaa aatcatcgtg aatttgaatc gccggtctcg cattcggcag
     6661 agggggcaag ttcacgagta cttgcgcggc tccgtgtggc tttgaaaagc ggatcgggca
     6721 ctctcctccg attttatcac gtcaacggct gatttcgccg gattgacaag gggatagggc
     6781 gccagtaaaa tgcggccgcc tttggttttt tcctctcgca ccaaatttca tgtgtgtaaa
     6841 gggatacgtg tttttaacgc gcggtcgact gcgtagtacg ggataccgga tgcgatctgg
     6901 aagtatttaa gtgcccctca gggtgagcgg gtcaggaaaa ctccctgaca tctcacttaa
     6961 atggtctgtc atggcgctat cgtctagcct gatctaggac gtcaccctct caaggtgaaa
     7021 acacgggttt aaatcccgtt agcgccaggt ttttttcgaa gtgccgcgcg gagagaatgg
     7081 gttacacggt tttttaatct taacgttttt tatctttatt gaggtgcagt atgatcccgc
     7141 gatacacccg gccggaaatg gccaaggtct ggactgaaga aaataagttg cagaagtggc
     7201 tcgatgtgga gttggtagcg ctcgatgggc tcgcccgcta cggttacatt cccagggaca
     7261 ttccttccaa agttcgcgcg aaagctaaat ttaatattca gcgcgttaaa gaaattgaaa
     7321 aagttaccaa tcacgatgtg atagcgtttc tgacgaatgt cgcggaatat gtcgggcctg
     7381 ccggccgata cgtgcatttc ggattaacat cttctgacat tctggatacg gggctcgccc
     7441 tgcaactggt cgacgcttcc caaattctga aacgggaaat ccagaagctg atccatattc
     7501 tttcaaaaca ggctaagaag catgccggaa cccttatggt gggacgttct cacggcgttc
     7561 acgcggagcc gaccaccttt gggcttaaaa tggccctgtt ttatgccgag ttccagagaa
     7621 acctcgaaag gtttgagcgt gctaccgaga atatccggtt tggcaagatt tcgggcgcgg
     7681 ttggtacttt tgccaatgtg gatcccaaag tagaaaacta tgtatgccgt aagctcaaat
     7741 tagcgccggc tcccgtatca acccaaattc tccaacgtga ccgccacgcg gagtatatga
     7801 ccgttatcgc ggtgattggt ggttccctcg agaagcttgc caccgaaatt cgaggtcttc
     7861 agaaaaccga gtgctatgaa gtccaggaat atttcgcgaa agggcagaag ggttcttccg
     7921 cgatgccgca caaacgaaat ccgattacct gtgaacggat cgccgggctt gccagaattt
     7981 tgcgagggaa cgctctggcg gcgctggaaa atatcgcgct ttggcacgaa cgggatatca
     8041 ctcactcgtc cgtggagcgc gtgatttttc cggacagcac catcctgctg gattacatgc
     8101 ttggtttgat gcaggacgtg attgaaaatc ttcaagtcaa caaggagcgg atgctttcga
     8161 acatggagtc gagccgtggg atggtatttt cccaggggtt gcttctgaag ctggttgaaa
     8221 aagggttgac gcgtgaagac gcttacaagc tcgtccagaa cgccgcgaag cggatttggg
     8281 aggatgtaaa cgcgaatttg cgggaagtgg tgttgaacga taaggaaatc cggaagcatt
     8341 tatcagcgtc cgaaattcaa agcgtgttca gctataaacg ccatacccgg aatgtttccg
     8401 cgatcttgaa gcgggctggt gttttaaaaa ctcgcaagtg aagagttttg tttcttccac
     8461 cttaatttaa cggagacgtg atggtgaggg atcttgaagg gaacgctatt ctggagaccc
     8521 atttgcccct gccacgaatt gcggtcggga aggtgcgtga catttacggc gtgggaagcg
     8581 acaagctttt gatcatcacg accgaccggt tgagcgcgtt tgatgtggtg atgccgaccc
     8641 ccgttcccct gaaggggaaa gttcttaacc agatcactct cttttggcag aactattttc
     8701 gggatgtgat cccgcaacat attatcaccg ctgatgtcga cgagatgtcg ctcccggatg
     8761 aggtgatcaa gaattttcgt cacgaaatcg aaggccgctc catgctggtg agaaaggccc
     8821 aaccgcttga aatcgagtgc gtggtccgcg gttatattac gggttcgggc tggaaggatt
     8881 accaaaagac gggcatggtt tgtgggcata agctcctccc ggggatgaag cagtgcgaaa
     8941 aattcccgga gccgcttttt acgccgtcca ccaaatccaa agcggggcac gatgtcaata
     9001 tcgatttcgg cgaggcaatc cggatcgtgg ggaaagacgt cgcgatgcaa gtccgcgatc
     9061 tgacggtcgc gctctacaag aaggggcagg aatacgccgc ttccaagggg atcatcatcg
     9121 cggacacgaa gtttgaattt ggaattcgcg atggcgagat tatattgatt gacgagattt
     9181 taactccgga cagctcccgg ttttggcccg cggatcaata tgaaacaggc cacgatcagc
     9241 cgagttttga caagcagatt gtccggaatt atctggaaac gctcgcctgg gacaaacggc
     9301 ccccgggacc ggagcttccc ccggatatta ttgaaaaaac gacggcggct tattgtgaga
     9361 tctttaagag gctgaccggg aaggacattc ggcaggtttg aagttttgtc gaaacacaga
     9421 ttgaagcggt cgattggatt tatgcggagg ttgcctgttg acacgaagca attgatagtt
     9481 gaacggcaac catggatgag agggaagacc tcaaaatgaa aggaatcatc tgcgatgaag
     9541 tttaaagccc ggatttatac cagtttaaaa aaatccgtgc ttaatcccca gtggcagacg
     9601 acgttgcgcg tccttgcggt ctttgataaa accaaactgg tgtcttgcca cgaccttggt
     9661 gcgatttcct tcgcgatgga gagaagataa tgggcggcga aaacatcaaa caccattgcg
     9721 gtattttcgg gatttacaat catccgcagg ccgcgcgcct gacccagctc ggcctttttt
     9781 cactacagca tcgcggagaa gaggcggcgg ggatctgcgc ctcggacgga gaggatttgc
     9841 atcttgtgaa agggtggggg cttgtgggcg atgtttttga tgaaacctct ttctccagct
     9901 tgcgggggaa tcacgcgatc gggcatgttt gttattccat ggccggagcc tcgacgccgc
     9961 tgaacgcgca accttacaaa gttgattatt cgcggggaca gatcgcgatc gctcataacg
    10021 gtaatcttgt gaacgcccaa atccttcgcg cggaacttga ggcctacggt tcgatttttg
    10081 gaagcacgat ggattccgaa atccttatcc atcttatcgc aaagccgacc taccggacgc
    10141 acgaagaagg aatcgtcgaa agcgtgaaac gggtggcggg ggcgtacagt cttgtcatat
    10201 tgactgaaaa tcagctttac ggaatccgtg atccgcatgg cttcaggcct ctgtgtctgg
    10261 ggaaacttgg agatagctat gtcctcgcgt ccgagacctg cgcctttgac ttgatcgagg
    10321 ccaagtttgt ccgtgaaatt gaaccggggg aagtggtggt gatcgaccgg aaaggcgtgc
    10381 gtagccattt ccctcaaaag ggaacgcggg aggcgggagc ttcccccgcg cattgtattt
    10441 ttgagcacgt ttatttcgcg cgccctgatt ccagggtttt tcatgaaaat gtcggtttaa
    10501 tacgggaaaa gtttggacgc cagcttgcca aagagcatcc cgcggacgcg gacatcgttg
    10561 ttccggtacc cgactctggc acgttcgcgg cgatggggtt ttcaaaagaa tccgggatac
    10621 cgatggcttt tggttttatc cgcaatcact atatcggtcg cacgtttatc agcccgggcg
    10681 cgtcgaaccg atcgtttaag gtgaagatta aactgaatct gatacgtgaa attgtggaag
    10741 gaaaacgcgt ggtggtagtg gatgattcga tagttcgcgg aaatacggct aaaagcaggg
    10801 ttaaattact gcgcgccgcg ggcgccaaag aaatccatat gaggattagc tgtccgccgc
    10861 acgtgtcgcc ctgttattac ggaattgatt ttccttcaaa aaaagaactg ctcgcggcaa
    10921 attacacggt cgcggaaatt aaaaaatttc tcggggtcga aagcctgggc tatttaagcc
    10981 ttgaaggaat gctgggttcg gtgacgcggc cgcgtaagga tttttgcgtg gcctgtttta
    11041 ccgggaagta tccgacgccc atttatggcg ggcgaggtaa aaataaactt gaacaaaagg
    11101 cagtcaaatg ttgcggacat tgatccgcgg ggaaatatga aaaaaacgga caaaaaagag
    11161 tggacttacg aaaaagccgg tgtcccgcac ctgaaagggg acgaaactta caatcggaag
    11221 atcgtgagcc tgattcggtc gacgcatgtg aagggcgtga tggggagcgc gcttggcttc
    11281 gcgtcgcttt ttgatttgaa aaaatccggg ttcaaggatc ccctgttggt cgcgtccacg
    11341 gatggcgtgg ggacgaagct cgaactggcc cggctacgtt cccggcatga tacgatcggc
    11401 attgatcttg tggcgatgtg tgttaatgat ctgatctgct gtggcgcgaa accgctgttt
    11461 ttcctggatt attacgcgac ggggaaattt cagccttcgg tggtgggcga agttcttcgg
    11521 ggcgtgacgg atggttgccg gcaggccgga tgcgcgcttg tcggtggtga gacggccgtg
    11581 atgcccggat tttatggaga taaggcgccg gggcaggcgg cacaatatga catggcggga
    11641 tttgccgtcg gggcggtgga tcgaaagaag gtaatctcag ggggaggaat tcgacaaggg
    11701 aacattgtgc tcgggattca gtcttcggga ttccattcga acggatattc gctcctgcgg
    11761 aaaatatttt cagacaagga attaaaaggt ccgatcggga aagagctttt gacgcccact
    11821 tttatttatg tcaggccgat attgagtctg atcaaacggg tcgcggtcaa ggggatcgtg
    11881 aatattacgg gcggcgcttt ttatgataat ctcccgaggg tgatccctca gggactgacc
    11941 gccgtgattg attctcgcgc gtgggacatt cctcctgttt tccggaaggc ccaaaaggcg
    12001 ggaaacattt cagattttaa catgttcaag acgttcaaca tggggattgg tatggtggtt
    12061 attttggaac ccggacaggt tcaacgggcg cgatccattc tcaaaacatt tcgcttgcgg
    12121 tcttggccaa tcggcaagat tattaaaggt caggaggtca aagttatatg agagtgcttc
    12181 tggtcggctc cggaggtcgg gaacatgcgc tggcatggaa aatcgcgcaa agtccgaaac
    12241 tgacgaagtt gttctgcgcg cccgggaatg ccgggattgc ggaacacgcc gagtgtgtcg
    12301 atatctcttc ggacgatatt gaaggtctcc tgaaatttgc gattgagcag gcgattgatc
    12361 tgacggtggt agggcctgag gctccgcttg tagcgggtct ttatgaccaa tttaatgccc
    12421 ggggattgaa ggtttttggc ccgtccgcga agatggctct tctcgaaggg agtaagtcgt
    12481 tttcgaaaga tgtaatgagc cggtttgggg ttcctaccgc ggcgtatgaa gtttttacaa
    12541 atgccaagga ggcgatgcaa tacactctcg agaccgaaat gccgattgtg atcaaggcgg
    12601 acggtctcgc ggccggcaag ggagtagtga tctgtgacac gtcggagcaa gctgtttcaa
    12661 ccttgtccaa gatgatggaa caaaaggttt ttggtccggc gggtaataaa gttgtgattg
    12721 agaaaaagat cgaaggggaa gaactttcga tccttgtgct gacggatggc gaaaccatcc
    12781 tgccgctggc aagcgcgcgg gatcataaac gcgcttatgg tcatgaccgg gggcccaata
    12841 ccggcggaat gggcgcttat tctcccagcg cccagtttga tgaaagtatg atcggctcga
    12901 tcattgatca gacgatacgc cctgtgattg atggactgag gaaagaggga tctccttacc
    12961 ggggtgttct ttacgccggc ttgatgatga ccgcggatgg gccttatgtg ctcgagtata
    13021 actgccgttt cggagatcct gaaaccgaag tcatattgcc gcgtctcaaa acggatctgc
    13081 tacctgtttt ggcggagatc gcggatggca aactttgcca gaaatccctt gaatggtatg
    13141 agaaggcatg catgaccgtg gtaatggctt cggggggata tcccggttct tatcaaaaag
    13201 ggctttctat tcatggtatt ccggcggcca aggcgatccg ggatgtcgtg gtgtttcacg
    13261 cgggaaccgc gctaaatgaa aaaaaggagg ttgtgacttc cggcggacgt gtgctggccg
    13321 taacggcgct cggggacaat cttcgcgcgg catatgatct ggcttatcag gcaatacaac
    13381 acatccactt tgaaggcgga tttttccgcc gggacatcgg tcgaaaggcc atggaggtgc
    13441 tcaaatgacc caggcgcttg tttcaattat catgggaagc gattcagatc tggatgtgat
    13501 gcaagagtgc gctgaagttc tcaaggagtt taacgtaccg ttcagtatgc aggttatgtc
    13561 ggcccaccga actccggaca ttgtcgcgaa gtttgcttct ttagcggcca aaaatggaat
    13621 cagagtaatc atcgccggcg cgggaggcgc cgctcatctg gcgggagcgg tggcggccca
    13681 taccacgttg ccggtcatcg gagttccgct tgcggcgacc gatttgagcg gcatggacgc
    13741 gttgctggcg acggttcaga tgccggcggg tattccggtc gcgacggtgg cggtcgggaa
    13801 acccggagca aaaaatgcgg cgctgttggc cgtgcagatt ttgagcctga cgaattctga
    13861 gctggcgaaa gttctcgaaa gttataaaat caagttggcg gaatcctgcc agaagaaaaa
    13921 tgaaaagctt caggaaaaac taaaaaaaac ctgagccatc gacgaggtat cgattaccca
    13981 aaatgagtgt ccccgagatt ataaaacttg atccacggga tccggactgt gtccagctta
    14041 agcgggttgt ggacgccagt cgggaagggg aagtggtcgg atttcccacc gaaacggttt
    14101 atggcatcgg cgggctgatg agtgtgcctg gcgtcgagaa acgcctccag gaaatcaaag
    14161 gaagaaggga agggaagccg tttgcgtttc atatcggcga ttgggatatg atcgacaggc
    14221 ttggcgtggt tcaaactcct atggtgcgtt tcctgatgcg aagtttctgg cctggtccga
    14281 ttactctggt tgtgaaaaac acggcgggag aaactattgg agttcgtttc ccgagaaata
    14341 aagtcgcaag gcttttgatc cgggaatcgg gggagccttt tatcg
//
LOCUS       DAOSIO010000016        14218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_4371_length_14218_cov_4.264492, whole genome shotgun sequence.
ACCESSION   DAOSIO010000016 DAOSIO010000000
VERSION     DAOSIO010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 14218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14218
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4371_length_14218_cov_4.264492"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..1285
                     /locus_tag="PK590_01375"
     CDS             68..1285
                     /locus_tag="PK590_01375"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOG23278.1"
                     /translation="MKKIIFGLLVLTVLGCVFWFLNHDSSPVPATLVSVHKGAVSVYS
                     VDPKGEKEFLGTVREGESIRATSRREGPLEMFNSMKKIAPKAMATFMAMVDQTVAEKA
                     PGVRRRIFTEADLQKYYNNLMRGLGCSDGVVSVDSYGMMMAGKFTGIPIAVHWQLGTD
                     KMNYGAPYLTLNQVKVGGVELPAFLVKKLSADATKVLGKGFYNVELIDIAYRDKSIDV
                     EFRRLPTTQISSPESYLPAKGLLSEAEEEIQGKVESIPPAEQERIAQAVAMKQKGIQY
                     VTIDKPGKGVFVKHRDGGWIPAEDGMVILEQDEIRTAPNVSVDILLDEGKAGSLEIKG
                     GSLLKALDLKTNFATGETSTLLNLAVGKLLAKVEPLTGNSSFSVRTPSAISGVRGTSF
                     EVEIEEPSRKFPF"
     gene            1334..1897
                     /locus_tag="PK590_01380"
     CDS             1334..1897
                     /locus_tag="PK590_01380"
                     /inference="COORDINATES: protein motif:HMM:NF020295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOG23279.1"
                     /translation="MASDVSIKTEFTPNPDSLKFNVSAPLCGHGTVHFASAEESRKSP
                     LAAELFKIAYVKEVMIGPDHVTVTRNEKAETWGVVIPLVTEVIKSYLSCGKENPKRCD
                     QCADVVESPRNETERRIVQILEEQIRPALVRDGGDVVFCGFEGGIVKLQLKGACAGCP
                     SATMTLKHGIEAYLKEYIPEVKEVVRV"
     gene            2060..2236
                     /locus_tag="PK590_01385"
     CDS             2060..2236
                     /locus_tag="PK590_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23280.1"
                     /translation="MAKKNLKALSVKKVITFKIRNRKGYAAIYDRNLTEGRTIAQALA
                     RMAKAVKRMGYLLG"
     gene            2413..3396
                     /gene="trpS"
                     /locus_tag="PK590_01390"
     CDS             2413..3396
                     /gene="trpS"
                     /locus_tag="PK590_01390"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOG23281.1"
                     /translation="MSRVLSGTRPTGKLHLGHLVGALENFKKLQGHDSFFMIADLHAL
                     TTEYEHKTTIAEKTIEVVVDYLASGLDPDQCTIFIQSEVGEHATLGLLFSMITPLGWL
                     ERNPTYKDQLRELKGKDLHTHGFLGYPVLQAADIAIYKAEKVPVGEDQLPHLELTREI
                     VRRFHHLYGKEIFPEPEAVLTPIPRVPGLDGRKMSKSYGNCIDLSDTREEVIRKVKTQ
                     MVTDPARKRKEDKGDPEKCPVFFYHKFFNPEKTEAVAHACRNASRGCVDCKMEMCEKL
                     NLFMDPVRERRKTWMNKQKEIREILDKGAVKARSVAQQTLTEVSKAMGLLA"
     gene            3411..4316
                     /locus_tag="PK590_01395"
     CDS             3411..4316
                     /locus_tag="PK590_01395"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOG23282.1"
                     /translation="MEEGKNGRPVPETPPSRLPETGTVSGDTVDPLHVKLQAYDGPLD
                     LLLDLIRKNEMNIYDIPIAEITNQYLSYLQQMKQLDLEVAGEFIVMAATLIYIKSKTI
                     LPAEKEVEESDGEDPRAELVRKLLEYQAFKEAAKELGILQTERGKMFTRQISDYYLGD
                     MQPEDAGIDTFSANFFDLVMAFQNVLAKKKRENPHEVFEEVISIEEKIVQVQSFLMER
                     KKMKFSELFPDTWSRNELVATFLALLEIVKIRFARVFQDRTFGEIVIEKRDESEYKRT
                     SPEEIPSAEVSPVKENPPSDLNGDR"
     gene            4384..5253
                     /gene="scpB"
                     /locus_tag="PK590_01400"
     CDS             4384..5253
                     /gene="scpB"
                     /locus_tag="PK590_01400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOG23283.1"
                     /translation="MSQTVKEREKQAKMKTLRKELDREIQDQLEESGVLETLVDVQRY
                     KGEAIHEEQLKDPTVAKKVVEALIFASSKPLSPAEIRKATRVLTASQIEKIVLELKDE
                     YQRQERPFEIVEIAGGYELSTKKEFAPWILRIELQRKARQATQSALETLAILAYRQPL
                     TRAEIESLRGVDVSGVVSTLMEKNLIKIVGKKEVPGRPFLYGTTDKFLEHFGLKSLQE
                     LPSLSEIQQIVESSVKKEQLLGTTKIVDVPEESVSPRVEPEDSGTPEKEPGIESNNSE
                     VEQHGSDAPAKEN"
     gene            5222..6301
                     /gene="pheA"
                     /locus_tag="PK590_01405"
     CDS             5222..6301
                     /gene="pheA"
                     /locus_tag="PK590_01405"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOG23284.1"
                     /translation="MDLTRLRKKIDEVDSKIIGLLNDRTRLAREIGRKKIESGKAVYV
                     PEREFEIYQKIEQIAGKGELPGDALKAIYREIMSAALAMEKPLVIAYLGPEATFTHLA
                     ALSKFGTSVSYKPCASFAEIFREVEMDRADYGVVPIENSIEGAVNHTLDMFIDSELKI
                     CSEIFFEISHNLMTNASSLNQVKRVYSKAEVFGQCRLWIEANLHHAERMETSSTAAAA
                     GRAAREDGAAAIGSRLAATLHNLPVMAEAIQDIANNVTRFLVISKQVPAMTRHDKTSI
                     VVSIRDKVGALYEMLLPIKKFRINMTKIESRPSRKKAWDYYFFIDLEGHVENERVRKM
                     LEAMEPKVKFLKVLGSYPAMGRPKA"
     gene            6380..7489
                     /gene="hisC"
                     /locus_tag="PK590_01410"
     CDS             6380..7489
                     /gene="hisC"
                     /locus_tag="PK590_01410"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOG23285.1"
                     /translation="MIPFKKHIQDIRPYEPGKPIRELQREYGIRNVTKLASNENPLGR
                     PSTKAMRAMRKAIREVHLYPEGSCHYIAERLSQELDLPTDQIIFGNGSNEIIELVGRG
                     FLSEGDEVISSEMSFLVYPILTQVCGGRFQAVPVTRDYRCDLPGILARVTPKTKVIFI
                     ANPNNPTGTYVTAQEMEAFLAKVPPHVIVCLDEAYVDFVDAGDFPKMLNYVREGRPNL
                     VVIRTFSKAYGLAGLRIGYAAASKEMVAYLYKIRQPFNVNSVAQAAAVATLDDKFFLW
                     KTKWMVARGRKYFYRQFRRLGLEYLPSQANFILLNLKKDGNEVFQELLRSGMIVRAMG
                     AYKLPTWIRVTIGRRSENAQLIRLLKTNLENNQSS"
     gene            7563..8579
                     /gene="aroF"
                     /locus_tag="PK590_01415"
     CDS             7563..8579
                     /gene="aroF"
                     /locus_tag="PK590_01415"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOG23286.1"
                     /translation="MIIVLKKNATPQEIDHICTKVRELGLTPNVSRGVERVVIGVIGD
                     EEKLQVQPLEVFPGVEHVMSILKPYKLASRDYRTENSEFEMNHGVKVGGKKIVVMAGP
                     CSVENYDMLLNVAKAVKKSGASFLRGGAFKPRTSPYSFQGLGEEGLKYLRQAADETGL
                     LVCTEVMDTRQVELVSQYADMLQIGARNMQNFDLLKECGISKKPVLLKRGLSATVKDL
                     LMSAEYLLSKGNFKVMLCERGIRTFETSTRNTLDLNAIPVIKKETHLPVLVDPSHGTG
                     FRDYVAPMAMAGVAAGCDGLMVEVHECPEEAKSDGEQSLRPKQFDELMAKARAVARAV
                     GRDI"
     gene            8666..9499
                     /locus_tag="PK590_01420"
     CDS             8666..9499
                     /locus_tag="PK590_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOG23287.1"
                     /translation="MFKKIVIIGMGLMGGSLAAACRKKFPKTRIVGVTRNPGALALAL
                     KRKWIHEGTRDVLAGARDADCVVICTPVDSYLPQLEKLDRVVADRALVTDVGSVKKAI
                     HDLVNAKTWKKVSFVGAHPMVGSHERGINFASPGLYGEGMVILTRDSKTDRRSFQKAK
                     YFWGRLSKKTVVLDPRTHDKLIAEISHLPHALAASLARSVSTRSLSLAAAGFRDTTRV
                     AASDISVWRPIFQLNRRQLFSAMTRFEKELKHFKAVLRSDDPAALGRYLSRAQRIRQS
                     L"
     gene            9593..9769
                     /gene="rpsU"
                     /locus_tag="PK590_01425"
     CDS             9593..9769
                     /gene="rpsU"
                     /locus_tag="PK590_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOG23288.1"
                     /translation="MLKVDIGEHEPLEKALKRLKKKMEREGILKLLKARKHYEKPSEK
                     RRRKARTSKTRRPF"
     gene            10228..11727
                     /locus_tag="PK590_01430"
     CDS             10228..11727
                     /locus_tag="PK590_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23289.1"
                     /translation="MDFQTRQFIRENSEKKKPEEIARILGLKERHVRREIERQKAKQK
                     LSAPKQEAYGFVLPNLTHLLFVFFLFSVLIGAIYSNTLQASFHFDDNYYIRENLALRD
                     LANLRAIWLWDPPRFLPNLTFALNYHFGGVNVLGYHLVNILIHIAASLMVYFFMLALF
                     QTPRLKSHSLSERSKDVALLAGLLFASHPIQIQAVTYIVQRHAAFATLFYVTTCFLYV
                     CARLRLNKKIYWVAVLFCIASMFTKQITFTLPIAILVIEFFLFPLDNSRFVKDRVKSL
                     TPFLVTIIIIPMLRLLEMQISPQKATQLFNTAFITPLHYLLTQFNVIREYLRFLFWPS
                     GQSIYHSFPISFTLMEPKTLLSLGLLIALFSTALALRKKQPAITFGIFWFFLTLFVES
                     SILPIAHVIFEHRMYLPMVGLCLAFSALLVSIIKSRKVLGVIAVFFVAALCFVTYQRN
                     EVWKTDESLWKDAAKKYKNEKRQLTYDEGVIVRTRAEDVPFSEWKHDAQ"
     gene            12350..13630
                     /gene="hisS"
                     /locus_tag="PK590_01435"
     CDS             12350..13630
                     /gene="hisS"
                     /locus_tag="PK590_01435"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOG23290.1"
                     /translation="MASKFQGFPGMDDILPGEIEKWQWVEEKARIFMEAGGFREIRTP
                     ILEPTELFIRSIGQSSDIVHKEMFSFKDRGERDMTMRPEMTASVARAVLEKGLLTQAK
                     SLRLYYMGPMFRAERPQAGRKRQFHQIGAEIINEGREADFESIALLHGFLQYAGLKEF
                     ALHVNDLGGEEDRDRVRVALVKYFGREKEKLCKDCQWRLEKNVLRIFDCKIPSCQPVI
                     EKAPWEEFAPLSAGFQALIQKLAEREIACTVNRRLVRGLDYYNGVVFEVTAGGLGAQN
                     AVAGGGRYDCLYQDLGGAKTPCTGFSIGVERLISAVESRNGPHFASLREKTVYLAPLE
                     DDAAIAKVIRNTALGLRERGFRIETTHGVFSLSQHLKRADKVKARYAVIAGTDEMAKG
                     VLIVKDLANRTQQEVKMDEIVSFLSLSSAAGSRS"
     gene            13676..>14218
                     /locus_tag="PK590_01440"
     CDS             13676..>14218
                     /locus_tag="PK590_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOG23291.1"
                     /translation="MLRSVTCGELNKSFVGKTESLTGWVDTRRDHGNLIFVDLRDRYG
                     LTQVVFNPQVHAAMHKVAETLRSEYVIRVRGKVAERPAGTVNPKIPTGEIELQAEEVE
                     ILNASQQLPFELGEKNVGEELRLAYRFLDLRRRDMVESLTLRHRVTKLVHEYLDKQRF
                     IEVETPYLTKSTPEGARDFLV"
BASE COUNT         3550 a         3225 c         3958 g         3485 t
ORIGIN      
        1 aagagatagg ctatactttg gatgaaggta acaggcagtg aaataaattg acgaaaagga
       61 gagcgaaatg aaaaaaatca tttttggctt attagttttg accgttctgg ggtgtgtttt
      121 ttggtttttg aatcacgatt cgtctcctgt tcctgccacg ttagtaagtg tgcataaggg
      181 cgctgtcagc gtgtactcgg tggatccgaa gggagagaaa gaattcctgg gaaccgtccg
      241 ggaaggagaa agcatccggg cgacttcccg acgtgaaggg cccttggaaa tgtttaactc
      301 gatgaagaaa attgccccca aagccatggc gacctttatg gcaatggtgg accagacggt
      361 tgcggaaaag gccccagggg tgcggcgccg gatcttcacc gaggctgatc tgcaaaaata
      421 ttacaacaac ttgatgcggg gcctgggatg cagtgatgga gttgtttccg ttgattccta
      481 cggaatgatg atggccggga aatttacggg tatccccatt gccgtgcact ggcagcttgg
      541 caccgataag atgaattacg gagccccgta ccttaccttg aatcaggtca aggtcggtgg
      601 ggtggaactg cctgcttttc ttgtgaaaaa actttcagcc gacgcgacca aggttttggg
      661 gaaaggattt tacaacgtcg agctgattga tatcgcgtat cgcgacaaga gcattgatgt
      721 cgagtttcgc aggcttccga cgactcagat ttcctcacct gaatcttatc tccctgccaa
      781 gggtctcttg tccgaggccg aggaggaaat tcaggggaag gtcgagtcga tcccgccggc
      841 agagcaagag cgtatcgccc aggccgtggc catgaagcaa aagggaatcc agtatgtcac
      901 cattgataag cctggcaagg gtgtgtttgt aaaacacagg gacggcggat ggatccctgc
      961 agaggacggg atggttattc tggaacaaga cgagatcagg actgctccga atgtttccgt
     1021 ggatattttg ctggatgagg ggaaggcggg gagtcttgag atcaagggtg gcagtctgct
     1081 gaaagccctt gatctaaaaa ccaattttgc gacgggggag accagtacgc tactgaacct
     1141 tgccgttgga aagcttctgg cgaaggtcga accgttaacg ggaaactcca gcttttcggt
     1201 tcggaccccc agcgctattt cgggagttcg cggaaccagc tttgaggtag aaatcgagga
     1261 gccatccagg aaatttcctt tttaaagaaa agtcacctga cggttccctt aactctgagg
     1321 attgatttta gacatggctt ccgatgtttc catcaaaacg gaattcacgc cgaatccgga
     1381 ctctcttaaa tttaacgtta gcgcgccgct gtgcgggcat gggacggttc acttcgcttc
     1441 ggccgaggaa tcccgaaaat ctcctctcgc ggcggagctt ttcaaaatcg cgtatgtcaa
     1501 ggaagttatg atcggccccg accatgtgac ggtgacgcgt aatgagaaag ccgagacgtg
     1561 gggcgtcgtc attcctcttg tgacggaagt aataaagtct tacctttcct gcggaaaaga
     1621 aaacccaaaa cggtgcgatc agtgtgctga tgtcgttgaa agcccgagga atgagacgga
     1681 gcgacggatc gttcagattc ttgaagaaca aattcgtccg gctcttgtcc gcgacggggg
     1741 agatgtggtc ttctgcgggt ttgagggagg aatcgtcaaa ctccagctga agggcgcttg
     1801 cgccggatgt cccagcgcga ccatgacctt gaaacatggt atcgaagcct acctgaaaga
     1861 atatatcccg gaagttaaag aggttgtcag agtctgacac tgatttctcg ccgatcagtt
     1921 ttcccggatt ttggcgcgtg ccgcgcgaga ccttcatcct ttttgacaaa cgccgataca
     1981 taaatacccg ttcacaatgg aagtcgttgg caggggtgaa tctggtatga ccgtcacttg
     2041 ggcacgacag gaggaaccta tggctaaaaa aaatctgaaa gcgcttagcg taaaaaaagt
     2101 gataaccttt aaaatccgca atcgaaaagg atacgccgcg atttacgatc gcaatctgac
     2161 ggaaggacgg acgattgccc aggctctggc ccgcatggcc aaggccgtga agaggatggg
     2221 gtaccttctg ggctgacgct ccacgaaaga aaacggtttg ggggtgatgg atcctcgacg
     2281 gcaggagacg cgccaggtcg tttcgttatc cgttttccgg tccatgttat ccgtgtttgg
     2341 ggttgtttga caccattcac cccatccttt aatatacgat ccattcatca cttacggagg
     2401 atccacacaa ctatgtcacg tgttttaagc ggtactcggc ctacgggcaa gctccatctt
     2461 ggtcatctgg taggcgcttt ggagaatttt aagaaacttc aggggcatga ttcctttttc
     2521 atgattgcgg accttcacgc cctgaccacc gaatatgaac acaaaacgac gatcgcggaa
     2581 aagacgatcg aagtggtggt ggactatctg gcgagcggtc ttgatccgga ccaatgcacg
     2641 atttttatcc agagtgaggt gggagagcac gcgacactgg gccttctttt ttccatgatt
     2701 actcccctgg ggtggctcga gcgtaatccg acttacaagg accaacttcg tgaactgaag
     2761 gggaaagatc ttcacacgca tggttttctg ggttatcccg ttctccaggc cgcggatatt
     2821 gcgatttaca aagctgaaaa agttccggtg ggagaggatc aattgccgca tcttgagctg
     2881 acccgtgaga ttgtccgccg gtttcatcat ctctacggaa aagaaatttt tccggaaccg
     2941 gaagccgttt tgactccgat cccgcgcgtg cccgggcttg acggccgaaa gatgagtaag
     3001 agttacggaa attgtattga tctgtcggat acgcgcgaag aagttatcag gaaagttaaa
     3061 acccagatgg tgacggatcc cgcgcgcaag cgcaaagaag acaaagggga tcccgaaaag
     3121 tgcccggtct ttttttatca caagtttttt aatccggaaa aaacggaagc ggtggcgcac
     3181 gcctgcagaa acgcttcgcg cggctgtgtg gactgcaaga tggaaatgtg cgaaaaacta
     3241 aacctgttta tggacccggt acgagaaaga cgtaaaacat ggatgaacaa acagaaagaa
     3301 attcgggaga ttctggacaa gggagcggtg aaggcgagat cagtcgcgca gcaaaccctg
     3361 acggaagttt ccaaggcgat gggacttctg gcgtaaaggg gacggattct ttggaggaag
     3421 gaaaaaatgg gcgccctgtt cctgagacgc ctccttcgcg actgccggag accgggacgg
     3481 tttccggaga caccgtggac ccgcttcacg taaaacttca ggcttacgac ggccccctgg
     3541 atcttcttct tgacctgatc aggaagaacg agatgaatat ctatgatatt ccgatcgctg
     3601 aaattacgaa tcaatacctg agttaccttc agcagatgaa acagctggat cttgaagttg
     3661 ccggtgaatt tatcgtgatg gcggcgaccc tgatctacat caagtcgaag acgatcctcc
     3721 cggccgaaaa agaagtggaa gaaagcgatg gggaagatcc gcgggccgag ctcgtgagga
     3781 aactgcttga ataccaagcc ttcaaggaag cggcgaagga attggggatt ctccagaccg
     3841 agcgcggaaa aatgttcacg cgtcaaatct cggattatta cctaggagac atgcaacccg
     3901 aggacgccgg aattgacacg ttcagcgcca atttttttga cctggtgatg gcgtttcaga
     3961 acgttctcgc gaagaaaaaa cgtgagaatc cgcacgaggt ttttgaagaa gttatttcca
     4021 tcgaagaaaa gattgtccag gtgcaatctt tcctgatgga gcgaaaaaaa atgaaattta
     4081 gtgaactctt ccctgataca tggagccgga acgagcttgt ggctactttt ctggcgctcc
     4141 ttgagatcgt caagatccgt ttcgcgcgcg tgtttcaaga ccggactttt ggcgagatcg
     4201 tcattgaaaa gagggatgag agcgagtata aaagaacttc acccgaggag attccttcgg
     4261 cggaggtgtc tccggtgaaa gagaacccac cgtcagatct taacggagat cggtaacttt
     4321 taacggaaaa gcggatcggg aaaaccgctg gatgtcaaac gttgaacgcg gagaatggac
     4381 gggatgagtc agacagttaa ggaacgggaa aaacaggcta aaatgaaaac gctccggaag
     4441 gagcttgatc gtgaaattca ggatcagctt gaggaaagcg gcgttctgga aactcttgtg
     4501 gacgttcagc gatacaaggg agaagcgatt catgaagaac agctgaaaga tccgacggtg
     4561 gcaaaaaaag ttgttgaggc gttgattttc gcgtcctcga aacctctttc gcccgcggaa
     4621 atacgaaaag ccaccagagt cttgacggct tcccagatag agaaaatagt cctggagctg
     4681 aaggatgagt atcaacggca ggagcgacct tttgaaatag ttgaaatagc cggaggttac
     4741 gagctttcga caaaaaaaga atttgcgccg tggattttga gaatcgagct tcagcgaaaa
     4801 gcccgccagg ccacccagtc cgcgctggag acacttgcca ttctcgcgta ccggcaacct
     4861 ttgacccggg ccgaaattga gtcgcttcgc ggagtggatg ttagtggcgt ggtgagtacg
     4921 ctgatggaga aaaatttaat caagattgtg gggaaaaaag aagtcccggg ccggcccttt
     4981 ctttacggga cgacggataa gtttctggag cactttggac ttaaatctct gcaagagctt
     5041 ccgagtctga gtgaaattca gcagatcgtg gaaagttcgg ttaaaaagga acagttgctg
     5101 ggaactacca agatagttga cgttcccgag gaaagcgttt ctccgcgggt tgagccggaa
     5161 gattccggaa cgccggagaa ggaacctggg atcgaatcca acaacagtga ggtggagcaa
     5221 catggatctg acgcgcctgc gaaagaaaat tgacgaagtt gattcgaaaa ttatcggttt
     5281 actgaacgat cgtacccgac tggcgcgcga gattggccga aagaagatcg agtcgggaaa
     5341 agcggtttat gtccccgagc gtgaattcga gatttatcaa aaaatcgaac agatagccgg
     5401 taagggagag ttacccgggg acgcgctcaa agcgatttat cgcgagatta tgtcggcggc
     5461 cctcgcgatg gaaaaaccgc ttgtgatcgc gtaccttgga cccgaagcga cgtttacgca
     5521 tctcgcggcc ctttccaagt ttggaacgag cgtttcatat aaaccgtgcg ccagtttcgc
     5581 ggaaatattt cgcgaggtgg aaatggatcg tgcggactac ggagtggtcc ctatcgaaaa
     5641 ctccattgaa ggggcggtga accacaccct tgacatgttt attgattctg aactcaagat
     5701 ctgttcggaa atcttttttg agatctccca taacctgatg accaacgcgt cgtctctgaa
     5761 ccaggtcaag cgcgtctatt ccaaggcgga agtttttggc caatgccggt tgtggattga
     5821 ggccaattta catcacgcgg aacggatgga aacgtcgagt accgcggcgg cggccgggag
     5881 agcggcccgg gaagacgggg ccgcggcgat tggctcccgg ttggcggcga ccctccacaa
     5941 tcttccggtg atggcggagg ccattcagga catcgccaat aatgtgaccc ggtttctggt
     6001 catttcaaaa caggttcccg cgatgacccg gcacgacaag acgtcgatcg tggtgtcgat
     6061 tcgtgacaag gttggcgcgt tgtatgagat gcttcttccg attaaaaaat tccggattaa
     6121 catgacaaaa attgaatcgc gtccgtcgag aaagaaggca tgggattatt atttttttat
     6181 tgatcttgaa ggccacgtgg aaaacgaacg tgtccgaaaa atgctcgagg cgatggaacc
     6241 aaaggtcaag tttctaaaag tcctcggttc ttatcccgct atgggaaggc cgaaggctta
     6301 aattgaaaga gggccctgga cacgcccatg cgccacgatc ccgtggctta tggcttgagg
     6361 cgtgggggcc gggtcaaata tgattccgtt taaaaaacat attcaggata tacgcccgta
     6421 cgaaccgggg aagccgatcc gggagcttca acgcgagtat gggattcgca acgtgaccaa
     6481 gctggcgtcg aatgaaaatc cgctcgggcg tccttcgaca aaagcgatgc gcgcgatgcg
     6541 caaggcgata cgcgaagtcc acctctatcc ggaaggaagc tgtcattaca tcgcggagcg
     6601 actgtcccag gaacttgatc tgccgacgga tcagattatt ttcgggaacg gatcgaatga
     6661 aatcattgaa ctcgtgggac gaggatttct ctcggagggt gacgaggtaa tttcgtcgga
     6721 aatgtctttt ctggtttatc cgattttaac gcaggtgtgc gggggccggt ttcaggccgt
     6781 tccggtaacg agggactatc gctgtgatct ccctggaatt ctggcccgcg ttacgcccaa
     6841 aaccaaagtt atttttatcg ccaatccgaa caatccgacg gggacttacg tgacggctca
     6901 ggagatggaa gcgttcctcg cgaaggttcc tccgcacgtg atcgtttgtc tggatgaggc
     6961 ttatgttgat tttgtggacg cgggggattt cccgaagatg ctgaattacg tcagggaagg
     7021 ccggcccaac ctggtagtga tcaggacatt ttcgaaggct tacgggcttg cgggccttcg
     7081 aatcgggtac gccgcggctt caaaagagat ggtcgcctat ctgtacaaga tccggcaacc
     7141 gtttaacgtg aactcggtag ctcaggcggc ggcggtcgcc acgctggatg ataaattctt
     7201 tttgtggaag accaaatgga tggtggctcg ggggcggaaa tacttttaca gacaattcag
     7261 gcgtctcggg ctcgaatatt tgccaagtca ggcgaatttt atcctgctca atttgaaaaa
     7321 agacggaaac gaggtttttc aggaactttt gcgaagcggc atgatcgttc gggcgatggg
     7381 agcttacaaa ctcccgacgt ggatccgggt cacgattggc cgccggagcg aaaacgcgca
     7441 attgatccgg ttgctaaaaa ccaatctcga aaacaaccag agttcgtaac ttgccgggcc
     7501 cgtgaacact tcagctgttt tgaagaggcg ctctgctgaa cccgtaaaga ggttaaaaaa
     7561 acatgattat cgttcttaag aaaaacgcga cgccccagga aattgatcac atttgtacca
     7621 aagtgcggga actcggtctg accccgaacg tgtctcgcgg tgttgagcga gttgtgatcg
     7681 gggtgatcgg ggatgaagaa aaattacagg ttcagccgct ggaggttttc ccgggggtag
     7741 aacatgtcat gtcgattttg aaaccctaca agcttgcgag ccgggattac cgcactgaaa
     7801 actcggaatt tgaaatgaac cacggcgtga aagtgggcgg taaaaaaatc gtggtgatgg
     7861 cggggccgtg ctcggtagaa aattatgaca tgcttttaaa cgtcgcgaag gccgtaaaaa
     7921 aatcgggggc ctcttttttg cgaggaggtg cgtttaagcc gaggacatca ccctattctt
     7981 ttcaggggct tggtgaagag gggctcaaat acctgcgcca ggccgcggat gaaacgggtc
     8041 tgctggtttg cacggaagtg atggataccc gccaggtcga attggttagc cagtatgccg
     8101 atatgctcca gattggcgcg cgaaatatgc agaattttga tcttctgaaa gaatgcggga
     8161 ttagcaaaaa accggtgctg ctgaaacgag gattgagcgc gacggtgaaa gatcttttaa
     8221 tgtccgcgga atatttgctg tccaagggta atttcaaagt catgctttgc gagcggggga
     8281 tacggacttt cgagacttcg acccggaata cgctcgacct gaatgccatt cccgtcatca
     8341 aaaaagaaac ccacctgcca gtcctggtgg atccttcgca cgggacgggt ttcagggatt
     8401 atgtcgcgcc gatggcgatg gccggggtcg cggcgggatg tgatggtttg atggtcgaag
     8461 tacacgaatg tccggaagaa gcaaaaagtg acggggaaca atcgttgcgg ccgaagcagt
     8521 ttgacgaact gatggcgaaa gcccgcgcgg tcgcgagggc ggtcggtcgg gacatttgag
     8581 atggtggtga gcaacgctca atgggttagc gtggcattgc gccctgaagg ctgtgcgggt
     8641 aaccatggat ggtgaaacga ctgctatgtt taaaaaaatt gtcatcatag gaatgggact
     8701 tatgggtggg tcgctggccg cggcttgccg gaaaaagttt ccaaaaacac ggattgtcgg
     8761 tgttacgcgc aaccccgggg ccctggcgct ggcgctcaaa agaaaatgga ttcatgaggg
     8821 gacaagggat gttctcgcgg gagcgcggga cgccgattgc gtcgtaattt gcactccggt
     8881 agattcgtat cttcctcaac ttgaaaagct tgatcgcgtg gttgccgatc gtgccctcgt
     8941 cacggacgtc gggagcgtca aaaaggctat tcacgatctc gtaaacgcga agacgtggaa
     9001 gaaggtatcg tttgtgggcg ctcacccgat ggtgggttcc catgagcggg gaattaactt
     9061 cgcatcgccc ggcctttacg gggaaggcat ggtgatcctt acccgtgatt caaaaacgga
     9121 tcgccggagt tttcagaagg caaaatattt ctggggtcga ctttcaaaaa aaacagtcgt
     9181 gcttgatccg cgaacgcacg ataaattaat cgccgaaatc agtcatctcc cgcacgccct
     9241 cgcggcctct ctggcgcgct ccgtttcgac gagatccttg tctcttgccg cggccggatt
     9301 tcgggatacg acccgggtgg cggcctctga catttctgtc tggcggccca ttttccagct
     9361 gaaccggcgc cagctttttt ctgcgatgac gcggtttgaa aaggaactga aacatttcaa
     9421 agccgttttg cgttccgatg atccggccgc tcttggtcgt tatctctcgc gggcacagcg
     9481 aattcgccag tccctctagc ggtattttga cattataatt ttctttgatt ccccaaaggc
     9541 tttccgtata ataccgcctc aaattaatcc acaccagaaa gggtgatcct gagtgttaaa
     9601 agtcgatatc ggtgagcacg agcctcttga aaaggccctc aagcgcttga aaaagaaaat
     9661 ggagcgcgaa gggattctta agcttcttaa ggcgcgtaaa cattacgaaa agccttccga
     9721 gaaaaggcgc cgcaaggccc gcacctccaa gacccgcaga ccattttaac ttgccggctc
     9781 gttggtactt tcctccttgg aacatcacgc gacgggggat tctgtcaaca cgggaatcct
     9841 tgagaactgc ttcacgaatt attgcgcatg acgtgagagt ctcactggtt gacgggtttc
     9901 gcgagagaga ccagaccctg gcggttacgc ggagctttca gaatgcgcac cgaaacctac
     9961 ccaacaccgg acgccgccta taaggagaca ttggtctccc aatccgccgt tttgttaatc
    10021 cctccctttg gatggcgttc ggtgtttgag tgtccagttc ttgccttcgg cgtgtgtgcg
    10081 ttgttgtttt tgtggtattc tctggaacgc gtgaagtgaa ccggtttatt tcgttttttt
    10141 gaaataatcg tagaggaaat gctttagaag tcttatctct ttttggatat aatggcacgc
    10201 ctacagagga aagacgccgg aacatctatg gattttcaaa caagacaatt tattcgagaa
    10261 aattctgaga aaaagaagcc ggaggaaatc gcccgaatcc tcggtcttaa agagagacat
    10321 gtcagaaggg agatcgagcg gcagaaggca aaacaaaagc tttctgcgcc aaaacaggaa
    10381 gcttatggtt tcgtccttcc aaatctcacc catcttttat ttgttttttt cttgttttct
    10441 gttcttatcg gtgcgattta ttccaatact cttcaggctt cttttcattt tgatgataat
    10501 tattatattc gcgagaacct ggcgcttcgc gatcttgcca atctccgagc gatctggcta
    10561 tgggatcccc cgcgattttt accaaacctc acgtttgcgt taaattatca tttcggaggt
    10621 gtgaatgttt taggttacca ccttgttaat atccttattc acattgcggc aagccttatg
    10681 gtttatttct tcatgttagc gcttttccag acccctcgac tgaagagcca ttctctttcg
    10741 gagcgctcaa aggatgtcgc ccttcttgcg ggacttttgt tcgcatctca cccgattcaa
    10801 attcaggctg tgacgtatat tgtgcaaaga cacgcggcgt tcgctactct attttatgtg
    10861 acaacttgtt ttttgtatgt ttgcgccagg ctgaggctca acaaaaaaat ctattgggtt
    10921 gcggttctct tttgcatagc atccatgttc actaagcaga ttacctttac cctgcccatc
    10981 gccattttgg taatcgaatt tttccttttc cctctcgata attcacgctt tgtgaaagac
    11041 cgcgtgaaaa gtcttacgcc tttccttgtg acgataataa ttatcccgat gcttcgtctt
    11101 ttagaaatgc agatttcacc acaaaaggct actcagcttt ttaatacggc ttttatcact
    11161 ccgctacatt atcttcttac ccaatttaat gttattcgag agtatctccg tttcctgttc
    11221 tggccaagcg gtcagtcgat ttatcattcg ttccccatct cctttaccct tatggaaccc
    11281 aagacccttc tttctcttgg gcttttaatt gctttgtttt ctacagctct agctttgaga
    11341 aaaaaacaac ctgccattac gtttgggatt ttttggtttt ttttaacatt gtttgttgaa
    11401 tcaagtattc tcccgattgc gcatgtcata tttgaacatc gaatgtattt gccaatggtc
    11461 ggcctctgcc tggctttttc agcattgctg gtctcgatca ttaagagtcg caaggtttta
    11521 ggcgtgatcg ctgtgttttt tgtagctgct ctttgctttg tgacgtacca gaggaatgaa
    11581 gtttggaaaa ccgatgagag cttatggaag gatgcagcaa aaaaatacaa gaatgaaaaa
    11641 cggcaactta cttatgatga aggagttatt gttcggacaa gagccgaaga cgtgcctttt
    11701 tccgaatgga aacatgacgc gcaataaggt tcacacagaa cttgttgaag gtgttgcaag
    11761 gcctcgttta tcttctctgc aaggttctat aatccccgct ccatggagcg ataaaatcgt
    11821 cactgaccat cttttcaagc cggacatttt gtatcctgtt tagcaagaat aagttgcgga
    11881 aaatcgaccc ttcaaagaca ggacaaaacg gacgaatttg acatcatttt gtccggttaa
    11941 atgtcgtctg tggagctgtt tccgaatttg gctggtaatc ggtggcgcgg gtagatatgg
    12001 tggtgttcca atcggatagg agggagaggt tctctattca tctcatcttg aaccgatcga
    12061 tttttaaagt ccggttcacg gaagatgttt tgttgtctca atcccagaaa tatcccttga
    12121 gacctgctac ctcatgttaa aaccttatca tgaggatcat ggttggcccg ttgcgatttt
    12181 atcgcgcgag ttgttggatt ctagcgattt tctcgtcgat tgtttttcgg agtttgaggc
    12241 ggacacaccc gggggattct gattatggaa aagacgatct ggttttggta tgatgcgtca
    12301 tcttttgaaa agtctggacg gaagaaagga cggaaggatc ggacacctta tggcatctaa
    12361 atttcagggt tttcccggca tggatgatat cctccccggt gagattgaga agtggcagtg
    12421 ggtcgaagag aaagcccgga tttttatgga ggccggcgga tttagggaaa tccggacccc
    12481 gattcttgaa ccgacggagc ttttcattcg ttcgatcgga cagagcagtg atattgtcca
    12541 caaggaaatg ttttctttta aagatcgggg cgagcgggac atgaccatgc gccccgaaat
    12601 gaccgcgtcg gtcgcccggg ctgttcttga gaaaggactt ctgacccagg cgaaatccct
    12661 tcggctttac tacatgggcc ccatgtttcg cgccgagcgt ccccaggcgg gtcgaaagcg
    12721 ccagttccac cagattggag ctgaaattat caatgaaggt cgcgaggcgg attttgaatc
    12781 gatcgcgttg cttcacgggt ttcttcaata cgcggggctg aaggagtttg cgcttcacgt
    12841 taatgatctc gggggagaag aagaccgtga cagggtccgc gttgcgcttg tgaaatattt
    12901 tgggcgggaa aaagaaaagc tctgcaagga ttgccagtgg cggcttgaaa aaaacgttct
    12961 ccgcattttt gattgtaaaa tcccgtcgtg ccagcctgtg atcgagaagg cgccatggga
    13021 ggagtttgcg ccgctgagcg ccgggtttca ggctctgatc caaaaactgg cggaacgcga
    13081 aatcgcctgt actgtgaacc ggaggttggt gcgcggcctg gattattata acggtgttgt
    13141 ttttgaagtc acggcgggtg gactgggcgc gcaaaacgcg gtggcgggcg gtggacggta
    13201 cgattgtctt tatcaggatc ttggcggcgc gaagactcct tgcacgggat ttagcatcgg
    13261 ggttgaaagg ctgatttccg cggttgaatc ccggaacggc cctcattttg cctctctcag
    13321 ggaaaaaacc gtttatctgg cgcctctgga agatgacgcc gccatcgcaa aggtgatcag
    13381 aaataccgcg ctgggactca gggagcgtgg cttccggatt gagacgacgc acggagtatt
    13441 ttcactcagt cagcacctga agagggcgga caaggtgaag gcgcgttatg cggtgatcgc
    13501 gggaacggac gaaatggcaa agggagtcct gattgtgaag gatcttgcca accgaactca
    13561 acaagaagtt aaaatggatg aaatcgtgtc ttttttaagc ctttcatccg cggcgggctc
    13621 ccgctcttga accgggcctg aaacggctga ttgataaaaa ggagaaagag gatttatgct
    13681 tcgtagtgtg acgtgcggtg aattaaataa aagttttgtc ggaaaaaccg aatccctgac
    13741 cgggtgggtg gacacccgcc gggaccacgg taaccttatt tttgtggatt tgcgcgaccg
    13801 ctatggcctg acgcaggtgg tttttaatcc gcaggttcac gcggcgatgc acaaggtcgc
    13861 cgagaccctt cgctcggaat atgttattcg tgtcaggggc aaagtcgcgg agcggcccgc
    13921 cggcaccgtg aatcccaaga ttccgacggg tgaaatcgag ctccaggccg aagaagtcga
    13981 aattctgaat gccagtcaac agcttccctt tgagctgggt gaaaaaaatg tcggggagga
    14041 gctgcgcctt gcttaccggt ttctggatct caggcggcgg gacatggtgg aaagtctgac
    14101 gctcaggcac cgtgtgacga agcttgtgca cgaatacctc gacaaacagc gttttattga
    14161 agttgagacg ccgtatttaa ccaaaagcac ccccgagggc gcgcgcgact ttttggtt
//
LOCUS       DAOSIO010000017        13823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_4526_length_13823_cov_4.583454, whole genome shotgun sequence.
ACCESSION   DAOSIO010000017 DAOSIO010000000
VERSION     DAOSIO010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 13823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13823
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4526_length_13823_cov_4.583454"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PK590_01445"
     CDS             <1..454
                     /locus_tag="PK590_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOG23292.1"
                     /translation="VLLRGDEVGDVMFYGRGAGPHPTASAVIADLLDIAKNNIEGPVD
                     DKTVIRALGNRIDIKNITSILSRYYLRFYVIDKPSVLARISKILGVCQVSISDVIQRE
                     RSEGKVVPLILLTHESPEKDVRTAIRSINRLPFVKGASQVIRMEEAGA"
     gene            487..1560
                     /gene="thrC"
                     /locus_tag="PK590_01450"
     CDS             487..1560
                     /gene="thrC"
                     /locus_tag="PK590_01450"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOG23293.1"
                     /translation="MSPKVAKRIGIIERYQEYLPVNGKTPIVSLNEGNTPLILAEALS
                     KELGLKNVRIYYKFEGLNPTGSFKDRGMTMAISKAVEAGSKAVMCASTGNTSASAAAY
                     ATRCGLKCFVLIPEGKIAKGKLVQAFRYGAKTIMIKGNFDQALELVKEITNDGKITLV
                     NSLNPYRIEGQKTAAFEIVDDLGDAPTYHAIPVGNAGNITAYWKGYQEYKKAGKSGIL
                     PKMLGFQAAGSAPIVLGHPVKNPETIATAIRIGNPASWKTAVAARDESGGLIDAVTDE
                     EITKAYRFIAEKEGVFAEPASAAGVAGVIKLAKQNFFKEGTVLVITLTGAGLKDPDFA
                     LSFNDPVDTIEANLDAVKEVVYS"
     gene            1557..2759
                     /gene="apgM"
                     /locus_tag="PK590_01455"
     CDS             1557..2759
                     /gene="apgM"
                     /locus_tag="PK590_01455"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOG23294.1"
                     /translation="MKYIVVSMSGAADHPAEELGGKTPLEVAKVPHLSFFTKAGKLGN
                     AKVFPDRVPLAPDASLFGLLGYGGNLYTGRGALEAANLELTLDDREVAFCMNFITEAS
                     GLLADSTAGSISTKESRALINFLNKKISSDFVRFFAGTAHRHIAVIKDAHGFEALSAK
                     TNPPEDVTGQKIEDVLPNGAGEELLKKLMFDARLLLQDHEINQVRVDLGENPANMIWL
                     WGQGKKPALKPLRELYDLTGGAMVAVREYAKGLGRVAGLTVMEVKEENEDPSVFYDRI
                     SKIALDALEEKDFVCIHLHQPDEASRAGDLKSKIFAIEGIDSFIFSKIRKYFERQKEA
                     RLLITPCHATLWKMRTAVRDSVPFTVFGKNIMADGVERFSEVTSKTSDLKITKNTELM
                     PFFITKVT"
     gene            2851..4068
                     /locus_tag="PK590_01460"
     CDS             2851..4068
                     /locus_tag="PK590_01460"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOG23295.1"
                     /translation="MALIVQKYGGSSVANSEKIMRVARRVILTKKAGHKVVVVVSAMG
                     DMTDDLIKLAEQINKNPEDRERDMLLATGEQVSISLLAMAIHKLGHKAVSFTGPQVGI
                     ITDAFHSKAHIQNISPGRIRKALEAGKIVIVAGFQGKTRDGEITTLGRGGSDLTAVAL
                     AAALRASCCEIYTDVDGIFTSDPRVIADAKKIDQITYDEILELASLGAKVVHSRAVEV
                     GKKFNMPIWVCNSHKNIRGTLITKETKKMEEVVVSGVALAEDEAKITLFRVPDRPGVA
                     AKIFQALADANVNVDMIIQNKTQTNSTDISFTVYKNELAKAIKVVQGNARKLGIKDIV
                     CDENIAKVSIVGIGMRSHSGVASKMFSTLAKHKVNIDMISTSEIKISVVVAGDKGKVA
                     ARAIHKAFGLGKK"
     gene            4114..5679
                     /gene="cimA"
                     /locus_tag="PK590_01465"
     CDS             4114..5679
                     /gene="cimA"
                     /locus_tag="PK590_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011012077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOG23296.1"
                     /translation="MKKKKILFYDTTLRDGSQMEGISLSLRDKLMIAKRLDRFGMHYL
                     EGGWPGSNPKDMEFFRAIKKAGLRHARVAAFGSTRRPHVKVKDDPQLKALLDAGTKTV
                     TIFGKSWNMHVRDVFKVSLKENLLMIYETVAYLKDKKIEVIYDAEHFFDGFKANPDYA
                     IKSLQQAAKAGADNLSLCDTNGGTLPHEIRRAILRVKRAVKTPLGIHCHNDSELAVAN
                     SIQAVGEGCALVQGTINGFGERCGNANLVSIIPNLQLKMGIQCFSPSKMKELTSLANY
                     VAGICNMPLMNNQPFTGRSAFAHKGGVHINAMTKNARTYEHIEPKAVGNYRRFLVSEL
                     GGKTNIMIKARELELHFQKDSPESKKILTEIQRLEYEGYQFEAAEASFELLVQRLLGN
                     VKPFFKIESVSVRTEAIGDNNPRSVADVKLTAKGEQHFESAEGDGPVNALDSALRKAL
                     RKAYPVIDEIHLTDYKVRVVNSEAGTAAKVRVFIEFQDKVKSWVTVGVHENIIEASWK
                     ALVEAIEYKLYKG"
     gene            5754..8513
                     /locus_tag="PK590_01470"
     CDS             5754..8513
                     /locus_tag="PK590_01470"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOG23297.1"
                     /translation="MNLDKQYDSREVEAKWYRLWEEKGYFRPRSDRDTDMAARDAYVI
                     VIPPPNVTGILHMGHALNNTIQDILTRWNRMKGKDVLWVPGVDHAGIATQNVVEKKLA
                     KEKKTRWDLGREKFLEEVWKWKQEHGHTITRQLRRLGASCDWTRERFTMDEGLSQAVR
                     EAFVTLYERGLIYRGNYIINWCPRCQTALSDEEAAHKDLQGGLYHIKYPLVGKQGPFE
                     KMGEDHVVVATTRPETMLGDTAVAVHPKDPRYQHLVGAKILLPLLDREIPVIADDFVD
                     PEFGTGIVKVTPAHDPNDFAMGLRHQLEQINVMTPDGKINENGGVYEGMDRFEARKKV
                     VHDLETLGLFIKRDTHLHAVGHCYRCDTVVEPYLSKQWFVKMRPLAEKALKAHEEGKT
                     VFYPDRWTKVYTNWLKNIRDWCISRQIWWGHQIPAWHCACCAEKSQSHGSQESHAARG
                     VRQEPGVIVSREKPSKCPTCGSTELKQDPDVLDTWFSSWLWPFSTLGWPEKTEDLKKF
                     YPTSDLVTAPEIIFFWVARMIMAGLEFMGEVPFRRINIHGTVRAANGLKMSKSLGNSI
                     DPLEVIEEMGADALRYSMIMLSAQDVYLSREKFEVGRNFTTKVWNACRFVLIQLEDMK
                     DASTDPDQFREMKLSLPSRWILSRLEGKTAEIEKYLSEFGLSQAASELYHFVWDDFCD
                     WYIELAKPMTQGRDIALKTETQKVLFFVCERMLRLLHPFMPFMTEEIWQKFNAKVTSC
                     GPESIMLAEWPREAEGRFHDTEAQKAVCLLQNAVSGIRDLRARFQIPPATKPKAIIAC
                     KEKSVMETFREFEREVKVLARLDSLELVDKFEKTGAMVANTFADFDVFISVEGLLDLE
                     MEKKRIQKKMAEAETWLASIQKKLANPNFAEKAPADVLEKEKEKLENAKKLLVSYQAQ
                     LAGLD"
     gene            8572..9369
                     /gene="nadC"
                     /locus_tag="PK590_01475"
     CDS             8572..9369
                     /gene="nadC"
                     /locus_tag="PK590_01475"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOG23298.1"
                     /translation="MNFLREALREDIGRGDVTSNLLIPANAWGSARVIAKEKGIFCGQ
                     RLVRELFRIADPAVKLEFKVRNGDRFRPGMVVFKMRGNIRSILKVERTMVNFISHLSG
                     ISTKTRLFVDKVKKYPVVILDTRKTTPGWRELEKVAVQVGGARNHRQGLDEYIFVKEN
                     HRIHGSLKKLRNCPGQFEIEVRSLGELWEAIQLKPQVILLDNFTPREAKKSVEIVRRE
                     TRKIFLEASGGITLDNVRQYAMAGVDSISVGSLTHSVEAVDFSLLVD"
     gene            9424..11448
                     /gene="uvrB"
                     /locus_tag="PK590_01480"
     CDS             9424..11448
                     /gene="uvrB"
                     /locus_tag="PK590_01480"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOG23299.1"
                     /translation="MNHFKLISAMTPKGDQPQAIEKLVKGFQAGKREQVLMGVTGSGK
                     TFTMAQIIAKLGKPTLVISHNKTLAAQLYSELKDFFPENAVEYFVSYYDYYQPEAYIP
                     HTDTYIEKDASINDDLDRLRLSATSSLLSRKDCVIVSSVSCIYGLGAPEDWQGLLITI
                     ARGELAERDKFLSGLIGLQYERVDTELKRGSFRVRGGRVDLFPSYGENPYRIDFGTEA
                     IERITLLDPVKFAAIREIDKLAVYPAKHFVTPTERLESALVSIEQEMKAQVRKLTGEG
                     KDLEAKRLESRTLYDLEMLKEVGYCGGVENYSRHLSGRAPGSTPYSLIDYFPDDFLVI
                     IDESHQSIPQIRGMYNGDASRKKVLVDHGFRLPSALDNRPLKFPEFENKIKQCLYVSA
                     TPGPYEMGRCPEVVEQVIRPTGLMDPVIEVRGTRGQIDDLIGEIRIRAKKSERTLVTT
                     ITKKMAEDLSRYLKDLGIKVSYIHSEFDAFERVEILRDLRLKKYDCVIGVNLLREGLD
                     LPEVSLVAVLDADKEGFLRSDVSLIQIAGRAARHVNGRVIMYAEKVTDSMRRAIDETA
                     RRRKVQEEYNRANGITPTGIIKEIREGIERWKKAEEFVHDVVHESKSEYQTKNYLAFL
                     KTRMEAAARALEFDKAARYRDEIRKLESDKTEKENPRKGKRKAPGKKRSR"
     gene            complement(11441..12472)
                     /gene="murB"
                     /locus_tag="PK590_01485"
     CDS             complement(11441..12472)
                     /gene="murB"
                     /locus_tag="PK590_01485"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOG23300.1"
                     /translation="MEIKEHYSLKEHHTFKIPVQARYFAEARTADEILELSRHPVFRS
                     QEKLVLGSGSNVFFTKNFEGLVIKIAIPGIEITKRQDGYAIIRAGAGVNWQELVDWSL
                     QQNFAGLENLSMIPGTVGASPIQNIGAYGIELKDVCEGLEAMDLESGEIREFTSEECR
                     FGYRNSIFKNELRGRYAILAVSLKVSDLSAPGVTYRPIASYGDLKTKLEAMDPKTLSP
                     RLVSETVSEIRRTKLPNSTLLGNAGSFFKNPVLAEERAKQLIKEHPQVPWFKDSQGIK
                     ISAGWLIEQCGWKGKRVGNTGCYEKQALVIVNYGAATAKEILAFAYNVQSSVRETFGI
                     ELAPEVNII"
     gene            complement(12538..12975)
                     /locus_tag="PK590_01490"
     CDS             complement(12538..12975)
                     /locus_tag="PK590_01490"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOG23301.1"
                     /translation="MRSHNRISQILSAWLPVILWAGLIFTLSGIPHLSSGWRYDFLLR
                     KIAHVTEYFILTLLTYRGLKLSWNTGPTPLNLATIAFVLIYALLDEIHQSFVPGRAGT
                     WQDVLFDASGIIIFFLLSKIWRGKIPSSEADNPCSPPEDPDGK"
     gene            complement(12962..13540)
                     /locus_tag="PK590_01495"
     CDS             complement(12962..13540)
                     /locus_tag="PK590_01495"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOG23302.1"
                     /translation="MEYRFNKNQPSRLLPLVAVILWAALIFSLSSIPGLHSGLSKDFL
                     LRKIAHVTEFFILSYLLYRAFATSFSLKKGLLFFCVGGLCLLYAISDELHQSFVPTRS
                     PAFIDVMIDSLGFFAFLAYFGSSRTRKTVVACAFCFLLICPTIATSGEKCAACDRARA
                     TASLPVTIEKLPEPAPEPEPGNSDKLLPHAES"
BASE COUNT         3465 a         3401 c         3943 g         3014 t
ORIGIN      
        1 ggtcttgctt cgcggagacg aggttgggga tgtgatgttt tacggccggg gggcgggacc
       61 tcaccctacc gcgagcgcgg tgatcgcgga tcttcttgat atcgccaaaa acaatatcga
      121 aggtcccgtg gacgataaaa ctgtcattcg cgcgcttggg aacagaatcg acataaagaa
      181 cattacctcg attctttcgc gctactatct ccggttttat gtcattgata agccgagcgt
      241 gctcgcccgg atttcgaaaa ttctcggggt ctgccaggta agcatctcag atgttatcca
      301 gcgggaacgg agtgagggga aagtggtccc cctcattctt ctgacccacg aatcaccgga
      361 aaaagatgtc cgcacggcca ttcgttcgat caaccggttg cccttcgtga aaggcgcgtc
      421 acaggtcata cggatggaag aagcgggagc ctgaacgcgc ggatcattaa cggtggaagg
      481 agtgatatgt cccccaaggt ggctaaaaga ataggaatta tcgagcgtta ccaggaatac
      541 cttcctgtga acggaaaaac cccgatcgtt tcattgaatg aagggaacac ccccctgatt
      601 ctcgctgaag cgctctccaa agagcttggc ctgaaaaatg tccggatcta ttacaagttt
      661 gaagggttga acccgacggg ttccttcaag gaccggggaa tgacgatggc gatttcgaag
      721 gcggtcgagg ccggctcgaa ggccgtgatg tgcgcgtcga cggggaacac gtccgcgtcg
      781 gcggcggcgt acgcgacccg gtgcggcctg aaatgttttg tcctgatccc ggaaggtaaa
      841 atcgcgaaag gaaaactggt gcaggccttc cgctacgggg caaagacgat catgatcaag
      901 gggaattttg atcaggccct ggagcttgta aaggagataa caaacgacgg gaagatcacg
      961 ctggtgaact cgctgaaccc ataccggatc gaaggtcaga agacggccgc gtttgagatc
     1021 gtggatgacc tcggagacgc gccgacttat cacgcgattc ccgtcgggaa tgccggtaat
     1081 atcacggcct actggaaggg gtatcaggag tataaaaagg ccgggaaatc cggaatactt
     1141 ccgaaaatgc tggggtttca ggcggccgga tccgcgccca tcgtgctggg acatcccgtc
     1201 aaaaatccgg agaccatcgc gaccgcgatc cggatcggaa atcccgcgag ctggaaaact
     1261 gccgtggcgg cccgtgacga atcgggcggg ttgattgacg cggtgacgga cgaggagatc
     1321 acgaaggctt atcggtttat cgcggagaag gaaggcgttt tcgcggagcc ggcatcggcg
     1381 gcgggagttg ccggagtgat caaactggca aagcagaatt ttttcaagga agggacagtc
     1441 cttgtgatta cgttgacggg cgccgggctg aaggatccgg atttcgcctt gagttttaac
     1501 gatcctgtcg atacgataga ggccaatctg gacgcggtca aggaggtcgt gtactcatga
     1561 aatacatcgt ggtgagcatg tcgggcgccg cggatcatcc cgcggaagaa ctgggcggca
     1621 aaactccgct tgaagtcgct aaagtcccgc atctttcctt ttttacgaaa gcggggaaac
     1681 tcggtaacgc caaagtgttt ccggatcgcg tgcctctcgc gcctgatgcg tcccttttcg
     1741 gactgctcgg ctacgggggc aatctttaca ccggccgggg agcgttagag gcggccaatc
     1801 tggagctgac cctggacgat cgcgaagtcg ctttttgcat gaactttatt accgaggcgt
     1861 ccggcttgtt ggctgattca accgcgggaa gtatttcgac caaggaatcc agggcgctca
     1921 ttaattttct caataaaaaa atatccagcg attttgtccg tttttttgcg gggaccgcgc
     1981 atcgccacat cgcggtgatc aaggacgctc acggctttga ggccctttcc gcgaagacaa
     2041 atccccctga agacgtgacg ggacaaaaga ttgaagatgt gcttccgaat ggggccggag
     2101 aagaattgct caagaaattg atgtttgacg cgaggttgct tctccaggat cacgagatca
     2161 accaggtccg cgtggatctt ggcgaaaatc cggccaacat gatctggctt tggggacagg
     2221 ggaaaaagcc ggccctgaaa cctttgcggg aactttatga tttgacgggt ggcgccatgg
     2281 tcgccgtccg ggaatatgcc aaggggcttg gccgcgtggc gggtttgacg gtcatggaag
     2341 tcaaggaaga gaatgaagac ccttctgttt tttatgacag aatttctaaa atagctctcg
     2401 acgcgctgga agaaaaagat tttgtctgca ttcatctcca tcagcccgat gaggcctccc
     2461 gtgccggcga tcttaaatcc aagatcttcg ccatcgaagg gattgattct ttcatatttt
     2521 ccaaaatccg gaaatatttt gagaggcaaa aagaggcgcg tcttctcatc accccttgtc
     2581 acgcgaccct ctggaagatg aggacggcgg tccgcgatag tgtcccgttt accgtgttcg
     2641 gaaaaaacat tatggccgac ggagtggagc gtttttcgga agtgacgtcc aaaacttccg
     2701 atctcaaaat tacgaagaac acggaactca tgcccttctt cataacaaaa gttacgtaac
     2761 gtgtgacgcc gcccaggaac cacgaaaaat cgcgtgtagc atcccgaaat atttccagat
     2821 caaagagaca tcaaattcag agaggaaaaa atggcgttaa ttgtacaaaa gtacggaggt
     2881 tcttcagtcg ctaattccga aaaaatcatg cgtgtcgcca ggcgggtgat tctgaccaaa
     2941 aaggccgggc ataaggtcgt ggtggtcgtg tcggcgatgg gagacatgac cgacgacctg
     3001 atcaagctcg cggagcagat caataaaaac cccgaagacc gtgaaagaga catgctgctg
     3061 gcgaccggcg agcaggtttc gatctcgctt ctggcgatgg ccatccataa acttgggcat
     3121 aaggcggtat catttaccgg tccgcaggtg gggattatta cggacgcgtt tcacagcaag
     3181 gcccacatcc agaatatcag tcctgggcga atcaggaagg cccttgaggc gggaaagatc
     3241 gtgatcgtgg cgggtttcca ggggaagacg cgggacgggg aaatcacgac tttggggcgc
     3301 ggaggctcgg acttgacggc ggtggcgctc gcggcggcgt tgcgggcaag ttgttgcgaa
     3361 atttacacgg acgtggacgg catttttacg tctgatccgc gcgtgatcgc cgacgcgaaa
     3421 aaaatagatc aaatcaccta cgatgaaatt ttggagctcg cgtctctcgg cgccaaagtc
     3481 gtgcactcgc gggcggttga agtcggtaaa aaattcaaca tgcccatctg ggtgtgtaat
     3541 tctcataaaa acattcgagg aactctcatc acgaaggaga cgaaaaaaat ggaagaagtg
     3601 gtcgttagcg gtgtcgcgtt agccgaagat gaagccaaga ttaccctgtt tcgggtgccg
     3661 gaccgccccg gggttgccgc gaaaattttt caggccctcg cggacgcgaa cgtgaacgtg
     3721 gacatgatta ttcagaacaa aacccagacg aactccacgg acatttcatt taccgtgtac
     3781 aaaaacgagc tcgcgaaagc catcaaggtc gtccagggaa acgcccggaa gcttggtatc
     3841 aaggacatcg tctgcgatga aaacatcgcg aaggtcagca tcgtcgggat cgggatgcgt
     3901 tcccattctg gcgtcgcgtc caaaatgttc tcgactctcg cgaagcacaa ggtcaacatc
     3961 gacatgatct caacttccga gatcaagatt tccgttgtgg tggccggaga caaggggaaa
     4021 gtcgcggcac gcgccatcca taaggcgttc gggttgggga agaaataaac ggccttgcct
     4081 gagttcggcg aaagccagac cggagaagat tgaatgaaga agaaaaaaat cctgttttac
     4141 gatacgactt tgagagacgg atcccaaatg gaagggatta gtctgtcttt acgcgacaag
     4201 ctgatgatcg cgaagcggct tgaccggttc gggatgcact acctcgaagg ggggtggccg
     4261 ggatccaatc cgaaagacat ggaatttttc cgcgcgatca aaaaagcggg ccttcgacac
     4321 gccagggtcg cggcctttgg ctccacgcgg aggcctcatg taaaggtcaa ggacgacccc
     4381 cagctcaagg ccctgctcga cgcgggaaca aaaaccgtca cgatttttgg aaagtcctgg
     4441 aatatgcacg tgagggatgt tttcaaggtg tcgcttaaag aaaatcttct gatgatttat
     4501 gagaccgtgg cttacctgaa ggataaaaaa atcgaggtta tctacgacgc ggagcatttt
     4561 tttgacggat tcaaggccaa tcccgattac gcgattaagt cgcttcagca ggccgcgaag
     4621 gcgggcgctg acaacctttc actctgcgat acgaacggcg gcacgcttcc ccacgaaatt
     4681 cgtcgcgcga tccttcgcgt caagcgggcg gttaaaaccc cgctcgggat ccattgccat
     4741 aacgactcag aacttgccgt ggccaactcg atccaggctg tgggggaagg ttgcgccctt
     4801 gtccagggga ccatcaatgg attcggggag cgatgcggaa acgcgaatct cgtgagcatt
     4861 atcccgaatc ttcagctcaa gatgggaatc cagtgtttct ctccgtctaa aatgaaagaa
     4921 ctgacgtccc tggccaatta tgtggcggga atttgcaata tgccgctcat gaacaaccag
     4981 ccgttcacgg gccgctcggc gttcgctcac aagggcggag ttcacattaa cgcgatgaca
     5041 aagaacgcca gaacttatga gcacattgag ccgaaagccg tcgggaacta ccggcgtttt
     5101 ctggtatctg aactcggggg caagacgaat attatgatca aggcacggga gcttgaactg
     5161 cattttcaga aagattcccc ggaatcgaaa aaaattctga ccgaaattca gcgtcttgag
     5221 tacgaaggct accagtttga ggccgcggaa gcgagttttg aactgttggt ccagcggttg
     5281 cttgggaacg tgaagccctt tttcaagatc gagtccgttt ccgttcggac ggaggccatc
     5341 ggcgataaca accctcgctc ggtcgctgac gtcaaattga cggcgaaggg ggaacagcat
     5401 tttgaatccg cggaaggcga cgggccggtg aacgcgcttg attccgccct gcgcaaggcg
     5461 ttgcgtaaag cgtatcccgt gatcgatgaa attcatttaa cggactataa agtgcgggtg
     5521 gtgaactctg aagccgggac cgcggcgaaa gtccgggttt tcattgagtt tcaggacaag
     5581 gtaaagtcct gggtcacggt gggtgttcac gaaaacatta tcgaagccag ctggaaggcg
     5641 ctggttgagg cgatcgaata taaattgtac aagggatagt gcggcgtgaa ggcatagggt
     5701 gcgaggagca aaagccttga cgccccgctc tttaccccgg acagcatact aatatgaatt
     5761 tagacaaaca atatgactcc agggaagttg aagcgaagtg gtaccgtctc tgggaagaga
     5821 aagggtattt caggccccgg agtgatcggg acacggacat ggcggcccgt gacgcgtatg
     5881 tcattgttat tcctcccccg aacgtaaccg gcatcctcca tatgggccac gccctgaaca
     5941 atacgataca ggacattctc acgcgctgga accgcatgaa aggcaaggac gtcttatggg
     6001 tcccgggcgt ggaccacgcg ggaatcgcga cccagaacgt tgttgagaaa aaactcgcca
     6061 aagaaaaaaa gactcgctgg gatcttggtc gcgaaaaatt tcttgaagaa gtctggaaat
     6121 ggaaacagga acacggccat acgattacgc ggcagcttcg ccgtctcggg gcctcttgcg
     6181 actggacacg cgagcgcttt acgatggatg agggtttgtc ccaggccgtg cgtgaagcgt
     6241 ttgtgacgct ctatgagcgc ggtctcattt accggggaaa ttacattatc aactggtgcc
     6301 cccgctgtca gaccgcgttg tccgatgaag aagcggccca caaggatttg cagggcggcc
     6361 tttaccacat caagtatcct ctggttggca aacagggccc ctttgaaaaa atgggagaag
     6421 atcacgtggt ggttgccacg acccgccctg aaaccatgtt gggagatacg gcggttgcgg
     6481 tccaccccaa ggacccccgt taccagcatt tagtcggagc gaaaattctt ttacccctgc
     6541 tcgaccggga aattcccgtc atcgcggatg attttgttga tccggaattc ggaaccggga
     6601 tcgtcaaggt gacgccggcg cacgatccta acgatttcgc gatggggctt cgccatcagc
     6661 tggaacagat taacgtcatg accccggacg ggaagatcaa tgaaaacggg ggtgtttacg
     6721 aagggatgga ccgttttgag gcccggaaaa aagtggtcca tgatcttgaa actctggggc
     6781 tttttatcaa gcgcgatacc catcttcacg ccgttggcca ttgttaccgc tgtgatacgg
     6841 tggtggaacc ttatctttcc aaacagtggt ttgtgaagat gcggccgctc gcggaaaaag
     6901 cgcttaaggc ccatgaagaa gggaagacgg ttttttatcc ggatcgctgg accaaggttt
     6961 atacgaactg gctgaaaaat atccgcgatt ggtgcatttc ccgccagatc tggtgggggc
     7021 atcagattcc ggcttggcac tgcgcgtgtt gcgccgaaaa gtcacaaagc cacgggtcac
     7081 aggaaagtca cgcggcgcgc ggcgtgcgtc aggaaccagg cgtgattgtt tcccgcgaaa
     7141 aaccgtccaa gtgtcccact tgtggaagca cggaactcaa acaggatccc gatgttcttg
     7201 atacctggtt ttcgagctgg ctttggccgt tctcgactct gggctggccc gaaaaaaccg
     7261 aagacctgaa gaaattctat ccgacgtcgg accttgttac ggccccggag atcatctttt
     7321 tttgggtcgc ccgtatgatt atggccggtc ttgaattcat gggggaagtg ccgttccggc
     7381 gaatcaacat ccacggaaca gtgcgtgccg ccaacggtct caaaatgtcc aagtcacttg
     7441 ggaactcgat cgacccgctt gaagtcattg aggaaatggg ggctgacgcc ctgcggtaca
     7501 gcatgatcat gctttccgcc caggacgtct atctttcccg cgaaaaattc gaagtcgggc
     7561 gtaattttac caccaaagtc tggaacgcct gccggttcgt cctgatccag ctggaggaca
     7621 tgaaagacgc gtccaccgat ccggatcagt ttagggaaat gaaattatca ctacccagcc
     7681 gctggatcct ttcccggctt gagggaaaga cggcggaaat tgaaaaatac ctttcggagt
     7741 tcggactctc ccaggcggcg agcgagcttt accattttgt atgggatgat ttctgcgact
     7801 ggtacatcga gctcgcgaag ccgatgacgc aagggcggga catcgccctt aaaaccgaga
     7861 cgcaaaaagt gcttttcttt gtatgcgaaa gaatgcttcg tcttctccat ccttttatgc
     7921 cgttcatgac ggaagaaatc tggcagaagt ttaacgcgaa agtaacgagc tgcgggccgg
     7981 agagcatcat gctcgctgag tggccgcgtg aggcggaagg ccggtttcat gataccgaag
     8041 cccaaaaggc ggtgtgcctt ttacagaatg ccgtaagcgg gattcgcgat cttcgggcgc
     8101 gttttcaaat tcctccggcg accaaaccca aagccatcat tgcctgcaag gaaaaatcgg
     8161 tcatggaaac gttccgggag tttgagcggg aagtcaaggt gttggccaga cttgacagtc
     8221 ttgagttagt cgacaaattc gagaaaaccg gagcgatggt cgccaatacg ttcgcggatt
     8281 ttgacgtctt tatttcggtt gaaggacttc ttgaccttga aatggagaaa aaacgaattc
     8341 agaaaaaaat ggcggaggcc gaaacctggc ttgcctccat ccagaaaaaa cttgccaacc
     8401 ccaactttgc ggagaaagct cccgctgatg tcctggagaa agaaaaagag aaacttgaaa
     8461 atgcgaaaaa acttctcgta tcctaccaag cacaacttgc cggtctcgac taaggcctca
     8521 cgcgccgatc gcgggatcca tggccattcc ctgccggttt caaggcggac tttgaatttt
     8581 ctgcgggaag cgctccggga agatatcggc cggggggacg tgacatccaa cttgcttatc
     8641 cccgccaacg cctgggggag cgcccgcgtg atcgccaaag aaaaggggat tttttgcgga
     8701 cagaggcttg tgagggagct ttttagaatc gctgatcctg ccgtgaagtt ggaattcaaa
     8761 gtccggaacg gggacaggtt ccgacccgga atggtcgtgt tcaagatgcg cgggaacatt
     8821 cgttccattt tgaaagtgga gcgaacgatg gtgaatttca tcagtcatct ttcgggcatc
     8881 tcgaccaaaa cccggctttt tgtcgacaag gttaaaaagt atcccgtcgt cattcttgat
     8941 acgcgaaaaa ccacgccggg ttggagggaa ctggaaaagg tcgcggtgca ggtggggggc
     9001 gcgcgaaatc accggcaggg gcttgatgaa tatattttcg tgaaagagaa ccaccggatt
     9061 cacggatctc tcaaaaaact ccgaaactgt cccgggcagt tcgagataga ggttcgcagt
     9121 ctcggggagc tttgggaggc gattcagctg aaaccccagg tgattctgct tgataacttt
     9181 accccgagag aggcgaaaaa atcggttgaa atcgttcgca gggaaacccg caaaattttt
     9241 ctcgaagctt cgggtggaat caccctcgat aatgtccgtc aatacgccat ggccggcgtt
     9301 gattcgattt cggtcggttc cctcacgcac tccgtcgaag ctgttgattt ctccctttta
     9361 gttgattaac acggtgtttt gccgttccct ccggaccacg gcgaatcggg tttagggtga
     9421 aatatgaatc atttcaaatt gatatcagcc atgaccccga agggcgacca gccgcaggcg
     9481 atcgaaaagc tggtgaaggg attccaggcg ggaaagcggg aacaggtgct gatgggcgtg
     9541 accggttccg gaaaaacctt cacgatggcg cagatcatcg cgaaactcgg gaaaccgacg
     9601 cttgtcatct cgcataacaa aacactggcc gcccagcttt attccgaact gaaagatttt
     9661 ttccccgaaa acgcggtcga gtattttgtc tcttactacg attattacca gcctgaagcc
     9721 tacatccctc acacggacac ctatatcgag aaagacgctt cgattaatga cgaccttgac
     9781 cgcctgcgtc tttcggccac gagctctctt ctttcccgca aagattgcgt cattgtctca
     9841 agcgtgtcct gcatctacgg gcttggagct ccggaggact ggcaaggcct gcttatcacg
     9901 attgccaggg gggagctggc cgaacgcgac aagttccttt ccgggctgat tggactccag
     9961 tatgagcgcg tggacaccga attgaaacgc gggtcgttcc gggtgcgcgg tggaagggtc
    10021 gatcttttcc catcgtacgg ggaaaatccg taccggattg attttggcac ggaagcgatc
    10081 gagagaatca cgctactgga tcccgtcaaa ttcgcggcga tccgggaaat tgacaaactc
    10141 gcggtttatc cggccaagca cttcgtgacg cccacggagc ggcttgaaag cgccctcgtg
    10201 tccattgaac aggaaatgaa ggcccaggtg agaaaactga caggggaggg aaaggacctt
    10261 gaggcgaaac ggcttgagtc tcgaactctg tacgatctgg aaatgctcaa ggaggttggc
    10321 tactgtggag gagttgaaaa ttattcgcgc cacctttcgg ggcgcgctcc gggctccacg
    10381 ccttattcgt tgattgatta ttttccggac gattttctgg tgatcattga tgaatcccac
    10441 cagagcattc cccaaatccg cggaatgtat aacggagacg cgtctcgaaa aaaagttttg
    10501 gtcgatcacg gcttccgcct tccctcggcg ctggacaatc ggccgctcaa attccccgaa
    10561 tttgaaaata agatcaaaca atgtctttac gtgtcggcca ctcccgggcc ttacgagatg
    10621 gggcgttgcc ccgaagtggt cgaacaggtc atccgtccca ccgggttgat ggacccggtg
    10681 attgaagttc gcgggacgcg gggccagatt gacgatttaa tcggagagat caggatccgg
    10741 gcgaaaaaaa gcgagcggac gcttgtcacg accatcacga aaaaaatggc cgaggattta
    10801 agccgctatc tcaaggacct tgggattaag gtgagctata ttcactccga atttgacgcc
    10861 tttgagcggg tcgaaatcct gcgcgacctg cggcttaaaa aatatgactg cgttatcggg
    10921 gtcaatcttc tccgggaagg tctggatttg cccgaggttt ctttggtggc ggtgcttgat
    10981 gccgataaag agggattttt gagaagcgac gtttcactga tccaaatcgc gggccgggcg
    11041 gcgcgtcatg tgaacggcag ggtgatcatg tacgccgaaa aggtgacgga ttccatgcgg
    11101 cgggccattg acgaaaccgc gcgtcggcgg aaagtccaag aggaatataa ccgggcgaac
    11161 ggcattacgc cgacgggtat cataaaggaa atccgtgaag gaattgaacg ctggaaaaag
    11221 gccgaagagt ttgttcatga cgtggtccac gaatcaaagt ccgaatatca aacgaaaaac
    11281 tatctcgcgt ttctcaaaac gagaatggag gcggccgccc gcgcccttga gtttgacaag
    11341 gccgcccgct atcgtgacga aatccgcaaa ctcgaatcgg acaagacgga aaaagagaac
    11401 ccacggaagg gcaaaaggaa ggcgcccggg aagaagcggt ctagataatg tttacctcgg
    11461 gcgcgagttc gatcccgaag gtttccctga cggaactttg cacgttatag gcgaaagcga
    11521 gaatttcctt ggccgtcgcc gcgccgtaat tgacgatcac aagggcctgt ttttcgtaac
    11581 agccggtatt tcccacccgt tttcctttcc agccgcattg ttcgataagc catccggcgg
    11641 aaattttaat tccctgggaa tctttgaacc agggaacctg ggggtgttcc ttgatgagct
    11701 gtttggccct ttcttccgcc aggacgggat ttttaaaaaa acttcccgcg tttccgagca
    11761 gggtggagtt gggaagtttc gtccgccgga tttctgaaac ggtttcactg accaagcggg
    11821 gagaaagcgt tttggggtcc atcgcctcca gttttgtttt caggtcacca taactggcaa
    11881 tgggccgata agtgacgccg ggagccgaga gatcggatac tttcagggac acggcgagga
    11941 tggcataccg ccccctgagc tcgtttttaa aaatgctgtt ccggtagcca aaccggcatt
    12001 cttcagaggt aaattcgcgg atttcgccgc tttcgaggtc catagcctca agtccctcgc
    12061 atacgtcctt cagctcgatc ccgtaggcgc ctatgttttg tatcggggaa gccccgacgg
    12121 tcccggggat catggaaaga ttttcgaggc ccgcgaagtt ttgctgaagg gaccagtcca
    12181 cgagttcttg ccagttgacc ccggcgcccg cgcggatgat agcgtacccg tcctgccgtt
    12241 ttgtgatttc gatcccgggg atggcgatct tgataaccaa accttcaaaa tttttcgtga
    12301 aaaagacgtt acttccactc cccaaaacaa gcttttcctg actacggaag acgggatgcc
    12361 gcgagagctc aaggatttcg tccgcggtac gcgcctcggc gaaataacgc gcctgcacgg
    12421 ggattttgaa cgtgtggtgt tctttcaaag aataatgctc tttgatttcc atagccaaat
    12481 tctaccttat cggcatgaat ttggcacgaa aagctttgga gatttccggt cttttagcta
    12541 tttcccgtct gggtcttcgg gaggggaaca tggattatcg gcctctgacg aggggatttt
    12601 tcctcgccag atctttgaca gaagaaaaaa tatgatgatg cccgaggcgt caaacagaac
    12661 gtcttgccag gttcccgccc ggccagggac aaaggactgg tgaatttcat ccagcagggc
    12721 gtagataagc acgaaggcaa tcgtcgcgag attcagtggc gtggggcctg tgttccaaga
    12781 aagcttcaac ccccgatagg tcaggagggt aagaatgaaa tattccgtga cgtgggcgat
    12841 ctttctgagt aaaaaatcgt aacgccatcc cgaggaaagg tgggggattc ccgaaagcgt
    12901 gaaaatcagt ccggcccaca ggatgaccgg gagccaggcg cttaaaattt gagaaattcg
    12961 attatgactc cgcatgaggg agcaacttgt cggaatttcc aggctccggt tctggcgcgg
    13021 gctcagggag cttttcaatc gtgaccggga gggacgcggt cgcccgggcc ctgtcgcagg
    13081 cggcgcattt ttcaccgctg gtcgcgatgg tggggcatat caataaaaaa caaaaagcgc
    13141 acgcgacgac tgttttcctg gtccgggagg agccaaaata ggctagaaaa gcgaaaaagc
    13201 cgagggaatc aatcatcacg tcaatgaaag ccgggctacg ggtcgggacg aaggactgat
    13261 gcagttcgtc cgagatggcg tagaggaggc ataacccccc gacacagaaa aagaggagtc
    13321 cttttttaag ggaaaatgag gtcgcgaacg cccggtaaag aagataagag agaataaaaa
    13381 attccgtcac gtgggcgatt ttcctcaaca ggaaatcctt cgaaagcccc gaatggaggc
    13441 ccgggattga ggaaagactg aaaattaacg cggcccagag aataaccgcg acgagcggta
    13501 aaagacgcga tggctgattt ttgttaaaac gatattccat ttctttatcc ttctttctta
    13561 cgctaatttg ggacgcgctt cccgttcact ttcggaagca agaaagcgcc gccggaaaaa
    13621 ccccaacggc tctttaacga ctggtagcag ggcaggattt aaacccacga tcccgaataa
    13681 ttgaccgcct ttgaccaaca ctaacccaac gccgcgggaa accaaagttt aacaggcgct
    13741 ttctgaaaaa gcaaataacc tcagataagc gagttgactc atattaaatc ttaccataga
    13801 gcgatcattg gccgaatcgt tga
//
LOCUS       DAOSIO010000018        13667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_4595_length_13667_cov_4.118645, whole genome shotgun sequence.
ACCESSION   DAOSIO010000018 DAOSIO010000000
VERSION     DAOSIO010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 13667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13667
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4595_length_13667_cov_4.118645"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..6449
                     /locus_tag="PK590_01500"
     CDS             57..6449
                     /locus_tag="PK590_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOG23303.1"
                     /translation="MAEPNRGKIDELEDLDHDLSILRKNEPELVKLTMEEIQETLALV
                     QNAITGRGLSLRSKIRSSPSDHPEGVLQTENQEGPQTGSDTLQISKNPDFPEIFDQVK
                     MLLKSYKISGRYFGDANIRSLLTIILDSRISDSVKRDKMRECLSSLLKPARQAYEQGK
                     LERAGQLLEVILSVNDKARSGASDPLLLELFKEAEEIGSNIQKIKTWLARRVIADNEG
                     TRVIRPKDLPVMLREWEAFSQKEREIIQRAYANGEELNWIEKWIEKTHNILIKSVRRR
                     SRKINNLQRFIVQGRTSLKKKPLAMAGMIVVNFIVDIFFIHPVLKVATIAGAIWLIKV
                     VGMGALQVVIAVGSAALLLIEGGLVRLFIIYPLFKLGYPKLLRINLKGLIVNFVPSGP
                     GNFAPFLFFNHDLGIDMPLALKERYLLSGQVERMLAYYPGIAEEIIVDITEAIYQAQL
                     KFGDSFNYRQFKIRRVEEEYIREGEESKDQLISYIKREDLSSHFPFRVKLKDRFFRWV
                     GFPAQGYHKGTRPTLFIGDSRQLKKIKQLLKNDGLSEKQLEGIFQLDLTKYFQKISVS
                     RKTVFLRKRPLAGEFIQPMFPPPKKNTHRSPKGKSKKPAQNHKGLESVRSGGDKKNRS
                     ELRQTSHQENQVPDVTWEELEQQGYGLDFNREQVKGKNFYKWVGGLKKDGRIIKGSSF
                     TLQVFPRYRLLCIESFFPAFPINDMNGKGRALLRKLLTFEDYTGFSVVSFATGKFPAS
                     LAKMKELHPVVDLTEAPWPVLLTESSDPEIRRKIESLYDRMIGIHAPFAERKEVLGAL
                     DEATFIGEVTASFYGLPAAREETQGRSVPRNSVESFDALSDRERMQVIIDAMKEFWHV
                     GALAGNTIREPSPSNIQMIEDLVRHFDIGTGRKKILETTGFGDGLTAAILAVATDADV
                     VGIEPDPARFKIGKAFLEFLEFKGIELPNLRLLQENLLDHEINDYDLIYFDYTPPVGG
                     RESFPQKVLTKMEKSLKAGSVFVAPRLGSEDPSSILDVETRIVRKFTAGSGLLAHRYD
                     RLGKISTSVFWKKESMNWGEVPGRSEVRSDIETADRDFARQFRKLDSARDEDSKVLMA
                     VREVMNQLPVFGKSETGPFGISRADKMILMNSDTDLGTFQKQVSRYFAEDENSRLRYP
                     EMMSWIRDRLDSAREEEAQTRRFSMESSHEERGVTDRAGELSGSPLSDPQGVVFSEND
                     LQEKMNMLFSKAGVYRRNMRGLITRKGIYFPSALDTVINVLKTMDLDRRKHAVILDAG
                     HGQGEFVFYLAKLIQSWYPDLEVELIGIEFEDEIFDESMRLLEVFKREGLSEKISIRF
                     HQGDFNSKDFEPYFQKADYVYYFSKGTNDIRSFARTLAETMNKPGAKIVEYGDGDLAE
                     YMQLGHAPVQSRMSPVSPRILVHEFISGQRGQRSESRSEQQGKGSGIFLQTRRVEYSD
                     GKYRLRNRNQSFSQWRGLARSVLRILETEFDNLVNSDISADREKEVKQILQNTLYGLA
                     PDVRQIVFSRNREIRRRLNALRNALASNNREVFLRVVRGLNQLRWLQEPELLSTVQLF
                     RGLRDISENKWHVVGSPQRMNGYLDQLEARLQRADDLGRLFQRFVLKYAELRSGKRFL
                     SHEEAQKEAFEATVTQGDFDFTRQSDNRTLLLFYQLIFVSSRQHPEFLESISRIAGQQ
                     AFRGPGRIPDEAPELATGIPNDLWMPQVVREALLKFEPLRLRSELRQSKETGKYFVLN
                     KPVGFITTEKQEPNAPDTPIAWDLLPPDVKNGRHFLGRLDKDTSGLLLVGGNAELNQL
                     LLNPKRNPDHKVQKTYEAVVSGHMTEDSANRLKQGVIIPAEGKKYLVHANELRILERF
                     REGKRPLTRLEIVISEGKFHQVRTMLRVVGHHVLELQRTAFGSILLGDLAPGSWRELS
                     LEEYSWLSGRLPQSPERSLGGVFEEEDAGGTSGKGSELRRSADDKVPGRHLSSSDFDT
                     KENIAELEKPAVFVIEKEQFERMSPGELRQMMVFAAINRKNLWLVIPDMLEGGNGQFL
                     EDLKSLGVRISRNLPEIAKSSGARVIGFSDRETDTAGKLRMRLGQKDPRLAKGVKEYF
                     AFEGGNGFFLAQLVSQPEDLAVMNGFRYDRTGRFQAALNAFLNHLMNSYVVTAISA"
     gene            6746..7756
                     /gene="rfaE1"
                     /locus_tag="PK590_01505"
     CDS             6746..7756
                     /gene="rfaE1"
                     /locus_tag="PK590_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HOG23304.1"
                     /translation="MDNTLSTAEKKRFLDIISGFRTARILVIGDFILDQFVWGNVSRI
                     SPEAPVPVVEVQRESYMPGGSLNVAHNIRSLLGLAYPCGVVGRDLYGRMLLKTMRQQG
                     IETGGIIYDLARPTTVKTRVIANSQQMVRFDREKAEDISRANLLKILKFVQQKIKMID
                     VIILEDYGKGVMQPFLVREVVKLARKNKKPVIVDPKEKHFSYYSGVTCITPNRKEAYG
                     GYERAVRPYQKTPTLERVGWGLMKKLRLNSVLITLGEDGMALFEKGGKITRVPTSARE
                     VYDVSGAGDTVIGVLALSLAAGAKMKEAAHIANIAAGIVVGKLGTATASQEELKEAIK
                     KS"
     gene            7857..8600
                     /gene="kdsB"
                     /locus_tag="PK590_01510"
     CDS             7857..8600
                     /gene="kdsB"
                     /locus_tag="PK590_01510"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOG23305.1"
                     /translation="MKALGVIPARLGSTRLPEKILREIAGKPMIQHVWENAGKARTLE
                     RVIVACDDEKIADCVRGFGGQAVMTRRDHPNGTSRIAEVIQKLDHEIVINIQGDQPLV
                     SPQALDEMVNLFRISRAPEMLTLALRMTDRDAFVNPNVVKVVCDEGGSALYFTRSPVP
                     YAQGEQDRNFSFLKHLGVYGYRREFLLEFVKWKPGILEMTEKLEQLRVLERGRKIRVI
                     ETDFDFISVDTEEDLREVERRLAKARSLK"
     gene            8685..10364
                     /locus_tag="PK590_01515"
     CDS             8685..10364
                     /locus_tag="PK590_01515"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOG23306.1"
                     /translation="MPKYIFVTGGVVSSLGKGIASASIAKILEAKGLKVAIQKLDPYL
                     NVDPGMMDPYQHGEIYVTDDGAETDLDLGHYERYTHSTYGRENSLTSGQVYHKVIMKE
                     RRGDYLGGTVQVVPHITDTIKDGIRDVSKGKRHDVVITEIGGTVGDIESLPFLEAIRQ
                     FRHEVGRENSLFIHLTLVPFIKAAGELKTKPTQHSVGTLREIGIQPDILLCRTEKKIG
                     QSIKAKIALFCNVDIESVIEARDVSSIYEVPLAFKEQGIDKVICQKLRIEYKAGSLHT
                     WKKDVVDIALRPRFSVKVAVVGKYTELQDAYKSIYESLRHGGIASDSQVIIKRINSEK
                     LKTERSLHALKGVAGILVPGGFGVKEIEGKLLAIQFAREKKIPFLGICLGMQCAVVEF
                     ARNVLNMKGANSTEFNKKTPFPVISLLEEQEKMKIPGDAMRRGAYPCEVKKDTKAWKA
                     YKTEQISERHRHRYEFNNTYREKFQKAGVKFSGVSPNGKLVEMIEIEDHPWFLACQFH
                     PEFKSKPDRAHPLFREFIASALEYQKANFPKSALEPVATRPDSEEKIIIHE"
     gene            10357..11190
                     /gene="kdsA"
                     /locus_tag="PK590_01520"
     CDS             10357..11190
                     /gene="kdsA"
                     /locus_tag="PK590_01520"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007958967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOG23307.1"
                     /translation="MNKVSLGKIIFGNPRQLVLIGGPCVIESEQGAFKLARAIQKITA
                     RLNLPYVFKASYDKANRSSIRSYRGPGMREGLRILARIKKDLGVPVLTDIHTPEQAEP
                     VAEVADILQIPAFLCRQTDLLVAAAKTGKIVNVKKGQFLSPWEVKNIVHKLEECGTKK
                     ILLTDRGSSFGYNNLVSDFRGIPVMRSLNYPVVFDATHSVQIPGGRGNSAGGAPQFIP
                     TLSRCAVVAGADAVFMEVHENPSRALSDGANALALKKLGPILKQLVALKKVVAAGGMV
                     S"
     gene            11187..12152
                     /locus_tag="PK590_01525"
     CDS             11187..12152
                     /locus_tag="PK590_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012823641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOG23308.1"
                     /translation="MKNLQKIGKEIINIETKALIRLRGKIGKNFEKAVLMMAHCHGRI
                     ILSGMGKPGFIARKLVATLASTGTPSLFLHPAEAVHGDLGMVTSKDLIIAISQSGETE
                     EIVRLLPILKKKGVPLIAMTSVPKSTLAKHAEVVLDLGVTREACPLNLAPSASTTASL
                     AMGDALALTLLKIKKFRSEDFAELHPGGSLGRRLLRVKDLMRTGKSNPVVKIGTPVRQ
                     VLIKITSARAGSCTIVNRKGRLIGIFTDGDLRRHLRQHGESILSRPIETVATLNPRVI
                     GGDKLAEEAFHILETLRIDEIPVVDKKMRVIGLLDVQDLMRAGIV"
     gene            12305..13243
                     /locus_tag="PK590_01530"
     CDS             12305..13243
                     /locus_tag="PK590_01530"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG23309.1"
                     /translation="MAKKKVYRYPLYLLARIAAMVPLILPRDWALALARAAGYAAYHL
                     IPRQRKKIIQNLEFAYAGEKTKNEIHRIASRVIGNLLETFVDFLRFAKLTREKAVAFI
                     DTGDSCNRCKDILQEGKGLIIITPHFGNWELLAGIFGLQGFQGAVIGRRIYYAPYNDW
                     IVGLRKSVGVETIYQTEAVRQVHRCLKKGEIVGLLPDQDIDKARGIFVDFFGKPAYTL
                     TAPVKFAMACGSPMLPAFMIRRPGNRYQLVLGDVIRPSAAGGNRDEAVKKYTEIWMRS
                     FEKIIRAYPEQWGWMHNRWRTTPEMIKSSQRKAVRK"
     gene            13240..>13667
                     /gene="lptC"
                     /locus_tag="PK590_01535"
     CDS             13240..>13667
                     /gene="lptC"
                     /locus_tag="PK590_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOG23310.1"
                     /translation="MIKKLIVGFIALVVFYVVFVQVQVVITRQEKAKQVTGEKAEDSV
                     HKVYSFQFAKYTPEGQKEIEIEGDSADIMADTVHLMNVMAKAYAEEVPVTVTADKGRY
                     DKKENLIHIEQNVVATTEDGTRLLTEELDMHPDRHVIENEV"
BASE COUNT         3600 a         2934 c         3760 g         3373 t
ORIGIN      
        1 cgattctttg ggcctccgac ctacaaattc ttcgggttct tcacccggat gtttttgtgg
       61 cagagcctaa tcgcggaaaa atcgatgaac tggaggatct ggatcatgat ctctcgatcc
      121 ttcgaaagaa tgagcccgag ctggtcaaac tgacaatgga agagattcag gaaacccttg
      181 ctcttgtcca aaatgctatt acgggcagag gtttgtctct ccgctcgaaa attcgctcat
      241 cgcctagcga tcatccggaa ggcgttctcc agactgaaaa tcaggaaggt ccccagacgg
      301 ggtctgacac acttcagatt tcgaaaaatc cggactttcc ggaaattttt gatcaagtga
      361 aaatgctatt aaaatcctac aagatctctg gacggtattt cggggatgca aacataagat
      421 ctcttttaac gataattcta gattcaagga tctctgatag cgtcaaacgg gacaagatgc
      481 gcgaatgtct gtcaagcctt ttaaaaccag cccggcaagc atacgagcag ggaaaactgg
      541 aacgtgccgg acagttgctg gaggttattc tatccgttaa tgacaaggcc cgttccggag
      601 cttcagatcc tttgcttttg gagttattca aggaagctga agagattgga tcaaatattc
      661 aaaaaattaa gacatggctc gcccggcggg taatagcaga taacgaaggg acgagggtga
      721 ttcggccaaa ggatcttccg gttatgctcc gggaatggga ggctttttct cagaaggaac
      781 gagaaattat tcaacgggct tatgcgaatg gagaggaatt gaattggatc gaaaaatgga
      841 tcgaaaaaac gcacaatatt ctgattaagt ctgtgcgaag acgatcgcgg aagatcaaca
      901 accttcagag attcattgtt cagggacgga cttctttgaa aaagaagcca cttgccatgg
      961 cgggcatgat agttgtcaat tttattgtcg atattttctt tatccatccc gttttgaaag
     1021 tagcgaccat tgccggggcc atctggctga taaaggttgt cggtatggga gcgttgcagg
     1081 tggtcattgc tgtagggtcg gcggcgcttc tccttataga aggggggttg gtgaggcttt
     1141 ttatcattta tcctcttttt aaactgggtt acccgaaact actccgtatc aatctaaaag
     1201 gtttgattgt taacttcgtc ccttcggggc caggtaattt tgcgccgttt ctttttttta
     1261 accatgattt agggatcgat atgcccttgg cgcttaagga gcggtatttg ctttcggggc
     1321 aggttgaaag gatgcttgct tattacccgg ggattgctga agaaatcatt gtcgacatta
     1381 cagaagccat ctaccaggcc caactcaaat tcggagattc gtttaactat cgccagttta
     1441 agattcgcag ggtcgaagaa gaatatattc gggaaggcga agaaagtaaa gatcaactca
     1501 tttcatatat caaaagagag gatctcagta gccattttcc atttcgtgtc aaattaaaag
     1561 atcgtttttt tcgatgggtg ggtttccctg ctcaggggta tcacaagggg acgaggccca
     1621 cgctttttat aggagactcc cggcaactta aaaaaataaa gcaacttcta aagaatgatg
     1681 gcctttcgga gaagcagctg gagggaatct ttcagctgga tctgacaaag tactttcaga
     1741 aaattagcgt ttccaggaaa actgtttttt tgagaaagag accgttggca ggtgagttta
     1801 ttcaaccgat gtttccaccc cccaagaaaa atacgcatcg atccccaaag ggtaaatcga
     1861 aaaaacccgc tcaaaatcac aaaggacttg agagtgttcg ttcgggggga gataagaaaa
     1921 atcgcagtga gctccgacag acgtctcatc aggaaaatca agtccccgac gtgacgtggg
     1981 aagaactaga gcaacaagga tacggcctcg attttaaccg agagcaggtg aagggaaaaa
     2041 atttttataa gtgggtgggt gggctgaaaa aagacggaag gataattaag ggttctagtt
     2101 ttactctgca ggttttcccc aggtatcggt tgctttgtat tgaatcgttt ttcccggctt
     2161 tccctatcaa cgatatgaac ggaaaaggtc gcgccctttt gcgcaagttg ctgacattcg
     2221 aagattacac agggttttca gtggtgagct tcgcgaccgg aaagttcccg gcatccctcg
     2281 caaagatgaa agagcttcac cctgtggtgg acttaacaga agccccttgg cctgttttac
     2341 tcactgaatc ttcggatcct gaaattcgaa ggaaaataga atcgctttac gaccggatga
     2401 taggcatcca cgcccctttt gccgagagga aagaagtgct gggcgcttta gatgaggcca
     2461 cttttatcgg ggaagtgacg gcctcgtttt acgggttacc cgcggcaaga gaagaaaccc
     2521 agggccgatc cgtgccacgc aattcggtgg aaagcttcga tgctttatct gaccgggaac
     2581 gaatgcaggt cattattgat gcgatgaagg agttctggca tgtgggagct ctcgcgggaa
     2641 atacaatcag ggagccctcc ccttccaata ttcaaatgat cgaagatctt gttcgccact
     2701 ttgatattgg aaccggacgc aaaaagattt tagaaacaac cggattcggc gatggcctca
     2761 cggcggcaat cttagctgtg gcaacggatg cggacgttgt gggcatcgaa ccggatcctg
     2821 cccgtttcaa gatcggaaag gcttttctgg aatttttaga attcaaaggg atcgaacttc
     2881 cgaatctccg gcttttacag gaaaatcttc ttgatcacga gatcaatgat tacgatctca
     2941 tttatttcga ttacacaccc cccgtgggcg gtcgggaaag ctttccccaa aaagtgctca
     3001 caaaaatgga aaaaagcctt aaagccggaa gtgtctttgt cgctccgagg ctgggtagtg
     3061 aagatccttc aagcatcctt gatgttgaga ctcgcattgt tcgtaagttt accgcgggat
     3121 ccgggcttct cgcccatcgg tatgaccgat tggggaaaat atcaacatcg gtattttgga
     3181 aaaaagaaag tatgaactgg ggtgaggttc cagggcgttc ggaagtacgg tcagacatcg
     3241 aaaccgcaga cagggatttt gcccggcagt tcagaaagct ggattctgca agggatgagg
     3301 attcgaaagt tttgatggct gtgcgtgaag tcatgaatca actgccggtt tttgggaaaa
     3361 gtgagactgg gccgtttggt atttcaagag cggataagat gatcttgatg aatagcgaca
     3421 cggatttggg aacttttcaa aagcaggtga gtcgctattt tgcggaagat gaaaacagcc
     3481 gattgcgtta tcccgaaatg atgtcgtgga tcagggatcg attggatagt gcccgggaag
     3541 aagaggcgca gacgaggcgg ttttccatgg aaagtagcca tgaagaaaga ggggttactg
     3601 acagggcggg cgaattgtca ggctcgccat taagcgatcc ccagggggtc gttttttcag
     3661 aaaacgacct gcaagaaaaa atgaatatgc ttttttctaa agcgggggtt tatcgcagga
     3721 atatgagggg gctcattacg cggaaaggaa tttattttcc gtcagctctg gatacagtca
     3781 tcaatgtcct gaaaacaatg gatcttgaca gacgtaagca tgccgtgatt ttggacgccg
     3841 gacacgggca gggagagttt gttttttatt tggcgaaatt gattcaatca tggtatcccg
     3901 atctggaggt cgaattgatt gggattgagt ttgaagatga aatctttgat gaaagtatga
     3961 gacttctgga agtgttcaag cgagagggtt tgtcggaaaa aatatccatc cggtttcatc
     4021 agggtgattt taacagcaaa gactttgagc catattttca gaaggccgat tatgtttatt
     4081 actttagcaa agggacgaat gacattcgct cttttgcccg tacgcttgca gaaacgatga
     4141 ataaaccggg tgcgaaaatc gttgagtatg gtgatgggga cctggctgag tatatgcagc
     4201 tcggccatgc tccagttcaa tcccggatga gtcccgttag ccccagaatc ctggtgcatg
     4261 aattcatctc cgggcagcgg ggacagcgtt ctgagagccg cagtgagcaa caaggaaaag
     4321 gaagcggaat ttttcttcaa accagacggg ttgaatactc agacggaaag tatcgtctca
     4381 ggaatcgtaa ccagagtttt tcgcagtggc gcggtcttgc ccgatctgtt ttgcgtatcc
     4441 ttgaaacaga atttgacaat ctcgtgaatt cggacatatc agcagaccgg gaaaaggaag
     4501 taaaacaaat ccttcagaat accctttacg gtttggcccc cgacgtaaga cagattgttt
     4561 tttcgcgaaa cagggaaatt cgaaggcgct tgaatgcttt gcggaatgcc ctggcttcaa
     4621 acaacaggga agtttttctg cgtgttgtcc gtggtttaaa tcagcttcgc tggttgcagg
     4681 aacctgaatt gcttagtacg gtccaattgt ttcgtggcct cagggatatc tcggaaaaca
     4741 aatggcatgt agttggttcg ccacagagaa tgaatggcta tttggatcag ttggaagcaa
     4801 ggctccagcg ggcggatgac ttgggacggc tcttccagcg gtttgtgttg aaatatgctg
     4861 aattaagatc agggaaaaga ttcctttctc acgaggaggc ccaaaaagag gcttttgagg
     4921 caactgtcac tcaaggtgat tttgatttta cccgccagtc tgacaatcgg accttgctcc
     4981 ttttttacca gctcattttt gtttcctccc ggcaacatcc ggaatttctg gagtccataa
     5041 gccggatcgc gggtcagcag gccttcaggg gcccggggcg gatacctgat gaagcccccg
     5101 agcttgccac agggataccg aatgatctct ggatgcccca ggttgttcgt gaggcattat
     5161 tgaaatttga acccttgcgt ttgcgttctg aactgcggca gtccaaagag accggaaagt
     5221 attttgttct aaataaaccg gtgggtttta tcacgacaga aaaacaggag ccgaatgcac
     5281 cggatacgcc catcgcctgg gatttgctac ctcccgatgt taagaacggc aggcattttc
     5341 tcggaagact tgataaagat accagcgggc tccttttggt gggaggaaat gccgaactga
     5401 accagttact gcttaacccg aaacgaaatc ctgatcataa ggtccagaaa acttatgaag
     5461 cggtagtgag cggacatatg acggaagatt cggccaatcg gctcaaacag ggggttatca
     5521 tcccggctga aggaaaaaaa tatctggttc acgcgaatga gctgagaatc ctggaacggt
     5581 tccgggaagg taaacggccc ttgacccggc ttgaaattgt gatatcggaa ggcaagtttc
     5641 atcaggtgcg aactatgtta agagtggtgg ggcaccatgt ccttgagctt caaagaaccg
     5701 cctttggttc gatcctttta ggggatcttg ctccgggtag ctggcgagaa ttaagtttgg
     5761 aggaatatag ctggctctcg ggacgcttgc cgcaatcacc cgaacgctca ttaggaggcg
     5821 tttttgagga agaggatgcg ggtggtactt cggggaaagg cagtgaacta cgacgaagcg
     5881 cggatgacaa ggtaccaggg cgacatcttt catcttccga ttttgatacg aaagaaaata
     5941 ttgccgagct agaaaaaccg gcagtattcg tgattgaaaa agaacaattt gagagaatgt
     6001 ctcccggtga acttcggcag atgatggtgt ttgcggcgat aaacagaaag aacctttggc
     6061 tggtcattcc ggacatgctg gaggggggaa acggccagtt cctcgaagat ttgaaatccc
     6121 ttggcgtccg catctccagg aatctgcctg aaatcgcaaa aagttccggt gccagggtga
     6181 tcggtttttc ggacagggag acggacacgg ccggaaaact cagaatgcga ttagggcaaa
     6241 aagatccgcg tctggcaaaa ggtgtcaaag agtattttgc gtttgaagga ggaaacggat
     6301 tttttctggc gcagcttgta tcgcaacctg aagatctcgc cgtgatgaac ggattccggt
     6361 atgacaggac aggtcgtttt caggcggcac ttaacgcctt cttgaaccac ctcatgaata
     6421 gctacgtcgt aacggctatt tctgcttaag ccccggagga aatcttttag cgctgggcgc
     6481 aatatttttt caagatagct gggtttgttc gtccctgata ttttgatgga tgcgaactcg
     6541 taacaaaacg tctcacctca ggccttaaaa taacggctaa cccgtttaaa taaaaggagt
     6601 tgtaaacttt ttccttcatt tgacacctct tgggcagggt gttaatatgt gcttttccta
     6661 ggggcaggaa gcatccaacc attgcccggt gtttagttcc tcaaaatgaa agtggaatac
     6721 cgggatctga ttttacaggg cacgcgtgga taacactctt tcgacagctg aaaaaaagcg
     6781 gtttttggat atcatcagcg gattcaggac ggctcgtatc ctcgtgatcg gtgattttat
     6841 tctcgatcag tttgtttggg gaaatgtctc ccgaatctcc ccggaagccc ccgttccggt
     6901 ggttgaagtc cagcgtgaat cgtatatgcc cggcggttcc ctgaatgtgg cgcacaatat
     6961 tcgttccctt ttgggtttgg cctacccttg cggggtcgtg gggcgtgacc tttacgggcg
     7021 gatgcttctt aaaacgatgc gccaacaggg cattgagacc ggaggtatca tctatgatct
     7081 cgcgcgtccc accacggtca aaacgcgggt gatagcgaat tcccagcaaa tggtgcgctt
     7141 tgaccgtgaa aaagcggaag atatttctcg cgccaatctt ctgaaaattc tcaaatttgt
     7201 tcagcaaaag atcaagatga ttgatgtcat tatcctggaa gattacggga aaggggtcat
     7261 gcagcctttc cttgtgcgcg aagtggtcaa gttggcccga aaaaataaaa agcccgtcat
     7321 cgttgatccc aaggaaaaac atttcagcta ttattcggga gtgacctgta taacgccgaa
     7381 ccgcaaagag gcttacggag gctatgagcg agcggtccgg ccctatcaga aaactccgac
     7441 gcttgaacga gtgggatggg gtcttatgaa aaaattgcgg ctcaattctg ttttgatcac
     7501 actgggtgaa gacggaatgg cgctttttga aaaagggggg aagattacgc gcgtccccac
     7561 gtcagcccgt gaagtttatg atgtctccgg cgcgggtgat accgtgatcg gggttctggc
     7621 gctgtctctt gccgcgggcg cgaaaatgaa ggaagccgcg catattgcga atatcgcggc
     7681 gggaattgtg gttggcaaac ttgggacggc gaccgcgagt caggaagagc tgaaagaagc
     7741 catcaaaaag tcatgagacg acaagtccga atcctgagtt acgaaacacg aagcgttatc
     7801 ggggcactga tgtcccaacg cggcaaacaa aagtttttga gttttcaagg atgcttatga
     7861 aagctcttgg cgtgattccc gcccggcttg gttctacgcg gcttccggaa aaaatccttc
     7921 gcgagattgc cgggaagccc atgatccagc atgtttggga gaacgccggg aaagcgcgaa
     7981 cccttgaacg ggtgattgtc gcctgtgacg atgagaagat cgcggactgc gtgagaggat
     8041 ttggcgggca agcggttatg acccggcggg atcatcctaa cggtacgtcg cggatcgcgg
     8101 aagtcataca aaaacttgac catgagatcg ttatcaatat tcagggagat cagccgctgg
     8161 tatctccgca ggcgctggat gagatggtaa accttttccg gatttcccgc gctcccgaaa
     8221 tgctaacctt ggcccttcgc atgaccgata gggacgcgtt cgtgaatccg aacgtggtga
     8281 aagttgtttg tgacgagggc ggaagcgccc tttattttac gcgttccccc gtgccgtatg
     8341 cccaggggga acaggatcgc aattttagtt ttttgaagca cctcggcgtc tatggatacc
     8401 ggcgggaatt tcttctggag tttgttaagt ggaaacccgg aatccttgaa atgaccgaaa
     8461 agctggagca actcagggtt cttgagaggg gacgaaagat tcgcgtcatt gaaacggatt
     8521 ttgattttat cagcgtggat acggaagaag acctgcgcga agttgaacga aggcttgcga
     8581 aagcccgaag tttgaaataa gcccgacttt cccgaacaca gtaaaaaata aattcaaaat
     8641 atttttttaa gatagcgggg aagaaagaga ggaaggaagt tgtcatgccc aagtatattt
     8701 ttgtgacagg cggcgttgtt tcgtcactcg gtaaaggaat tgcgtccgcg tctatcgcga
     8761 aaattctgga ggcgaaagga ttaaaagtcg ccatccagaa gcttgatcct tatcttaatg
     8821 tggatcccgg gatgatggac ccctatcaac acggggaaat ttacgtgacc gatgatggcg
     8881 cggagacgga ccttgatctg ggacattatg aacgttatac ccacagtacc tacggacggg
     8941 aaaatagtct tacgagcggc caggtttacc acaaggtcat catgaaagag cgccggggag
     9001 attatctcgg cggaacggtc caggtggtgc cacacatcac ggacactatc aaggatggta
     9061 ttcgggacgt gtcgaaaggg aaacggcacg atgtggttat taccgaaatc gggggcacgg
     9121 tgggtgacat cgaatcgctc ccttttcttg aagctatccg ccagttccgg catgaagtag
     9181 ggcgtgaaaa ttcactgttt atccatctca cccttgtccc ttttatcaag gccgcgggag
     9241 aactcaaaac gaagcccacc cagcattcgg ttggcacgtt gcgtgagatc ggtatacagc
     9301 ctgatattct cctttgccgg accgaaaaaa aaattgggca gtcgatcaag gcgaaaatcg
     9361 cgctgttttg taacgtggac attgaatcgg tgattgaggc gcgtgatgtt tcttccattt
     9421 acgaagttcc tctcgcgttt aaggagcaag gcattgacaa ggtaatttgt cagaagctca
     9481 ggattgaata caaggcggga agtctccata cctggaaaaa ggatgtggtg gatatcgcgc
     9541 ttcgaccccg tttttcggtg aaagtggcgg ttgtcgggaa gtacacggag cttcaggacg
     9601 cttataaatc tatctatgaa tcgctaaggc acggcggtat tgccagcgac agccaggtta
     9661 ttatcaaaag gattaattcc gaaaaactta agacggagcg ttcgcttcat gccttaaaag
     9721 gggtggcggg cattcttgtc cctggcgggt ttggggttaa agagattgaa gggaagttgc
     9781 tggcgataca gtttgcccgg gagaaaaaaa tccctttcct tggaatttgt cttggtatgc
     9841 aatgtgccgt ggtggagttc gcgaggaatg tgcttaacat gaagggcgcc aattcgaccg
     9901 agtttaacaa gaagactccg ttcccggtga tcagtttgct tgaagagcag gagaagatga
     9961 aaattcctgg agatgccatg aggcgggggg cttatccttg cgaagttaaa aaagacacga
    10021 aggcctggaa ggcgtataag accgaacaaa tttcggaacg gcaccgccac cgttatgaat
    10081 ttaataacac ctatcgcgaa aaatttcaaa aagccggcgt caaattctca ggagtttcgc
    10141 cgaacggaaa actggttgaa atgatcgaaa tagaggacca tccgtggttt ttggcctgcc
    10201 agtttcatcc ggagtttaaa tcgaagcctg accgcgcgca tccgctcttt cgtgaattta
    10261 tcgcaagcgc cttggaatat caaaaggcca attttcccaa atcagccctt gaacccgtcg
    10321 caacccgtcc ggacagcgag gagaaaataa ttattcatga ataaagtcag cttaggcaaa
    10381 attatctttg gcaaccctcg acagctggtc ctgattggcg gtccttgtgt tatcgagagc
    10441 gaacaagggg cctttaagct cgcaagggct attcagaaga ttacggcgcg acttaacctc
    10501 ccgtatgttt ttaaggcgag ttatgataaa gccaaccgct cttccatccg ttcgtatcgc
    10561 gggccgggca tgcgggaagg gctcaggata ctcgcgagaa tcaaaaagga cctgggagtt
    10621 cctgtcctga cggatattca tactcccgag caggcggagc cggtcgcaga agtcgcggat
    10681 atcctccaga tcccggcgtt tttgtgccgc cagaccgacc ttctggtcgc ggccgccaaa
    10741 accggaaaaa tcgtgaacgt gaagaagggg cagtttcttt ctccttggga agttaaaaac
    10801 attgtgcaca agcttgaaga gtgcggcaca aaaaaaattc ttttgacgga ccgtggctca
    10861 agcttcgggt acaataacct tgtcagtgat tttcggggaa ttccggtcat gcgctcgttg
    10921 aattatccgg ttgtttttga tgccacccac tcggtccaaa ttccgggggg acggggaaac
    10981 tcggcgggcg gcgcgcccca attcatcccg acgctttcgc gttgcgcggt tgtagcgggc
    11041 gctgacgctg tctttatgga agttcacgaa aatccgtccc gggccctgtc agacggcgcc
    11101 aatgccctcg cgctaaaaaa actggggcca attttaaaac agttggtagc gttgaagaaa
    11161 gttgtcgccg cgggaggaat ggtgtcatga aaaatcttca gaaaatcggc aaggaaatta
    11221 ttaatatcga gacgaaagcc ctgatccggc ttcgcgggaa aatcgggaaa aattttgaaa
    11281 aagccgtact gatgatggcc cattgccatg gccggattat tctaagcggg atggggaaac
    11341 ccggatttat cgccaggaaa cttgtggcga ctctggcttc gaccgggacg ccgagccttt
    11401 tccttcatcc ggcagaggcc gttcacggtg acctcgggat ggtcacgtcc aaagatttga
    11461 ttatcgcgat ttcccaaagc ggggaaaccg aagaaattgt aaggctgttg ccaatactca
    11521 agaaaaaagg cgtgccgctt atcgccatga ccagcgtgcc gaaatcgaca ctggcaaaac
    11581 acgcggaagt cgtgctggat ctgggggtca ctcgcgaagc ttgcccgctc aatctcgctc
    11641 cttccgcgtc gactacggcc tctctcgcga tgggggacgc gctcgcgctg accctgctta
    11701 aaataaaaaa atttcgaagt gaagatttcg cggagcttca tcccggcggc agtcttggaa
    11761 gacgcttgct tcgggtgaaa gacctcatgc gtacgggcaa gtcgaatccc gtggtcaaga
    11821 tcggaacgcc tgtcagacag gtgttgatca agattacgtc cgcgcgggcg ggaagttgca
    11881 cgatcgtgaa ccgcaagggt cggctgatag ggatttttac ggatggtgat ttaagacgtc
    11941 acctgcggca gcacggtgaa tcgattttga gccgtcctat cgaaacggtg gcgaccttaa
    12001 acccccgcgt gataggaggg gacaagcttg ctgaagaggc gtttcatatt ctcgaaaccc
    12061 tcaggatcga cgaaatcccc gtggtggata aaaaaatgcg tgttatcggg ttgctggatg
    12121 ttcaggatct tatgagagcg ggaattgtct gagaaaataa atcctgaaat ttgaaacatg
    12181 aatcaaaacg cgacacttta attaaaaagc cgaagcattt cattttgggg aactagaaac
    12241 attaatactt ggggtttgtt tcgggtttct tgcttcgaat ttcaggaatg atgcgacggg
    12301 ttttatggct aaaaagaaag tttaccgtta tccgctttac ttgttagcgc gaattgccgc
    12361 gatggttccg ctgattttgc caagagattg ggcgctcgct ttggcccggg cggcggggta
    12421 cgcggcgtat cacctgattc ctcgccagcg aaaaaaaatt attcaaaacc tggagttcgc
    12481 ctacgcgggg gaaaagacga agaatgaaat ccatcgtatt gcttccaggg tcatagggaa
    12541 tctccttgag acatttgtgg atttcttgag gttcgccaag ctgactcggg aaaaagctgt
    12601 ggcatttatt gatacgggag attcttgcaa tcgctgtaaa gatattttgc aagaagggaa
    12661 ggggctcatt ataataacac ctcacttcgg gaactgggag ctcttggccg gaatttttgg
    12721 actccaggga tttcaggggg cggtgatagg acgccggatt tattacgcgc cgtataatga
    12781 ctggatcgtc ggtctccgaa agtcggtggg tgtggagact atttatcaga ccgaggcggt
    12841 ccgacaggtt catcgctgtc ttaaaaaagg agagatcgta ggtcttttac cggatcagga
    12901 tattgacaag gcgcggggga ttttcgttga ttttttcgga aagccggcct acaccttgac
    12961 ggccccggtc aagttcgcga tggcatgtgg ttcaccgatg ttgcccgcgt ttatgatcag
    13021 gcgtccggga aaccggtacc agttggtttt gggggatgtg atacgtcctt ctgccgccgg
    13081 ggggaacagg gacgaagcgg tgaaaaaata cacagaaatc tggatgcgaa gtttcgaaaa
    13141 gataattcgg gcatatcccg agcagtgggg atggatgcat aaccggtggc gtactacccc
    13201 ggaaatgatt aaatcgagtc aaaggaaggc tgttcgtaaa tgataaaaaa attgattgtt
    13261 ggatttatag cccttgttgt tttttatgtg gtttttgtgc aggttcaggt ggtgattacc
    13321 cggcaggaaa aagcgaaaca ggtcacggga gaaaaagcgg aggattccgt ccacaaggtc
    13381 tattcctttc agtttgcgaa atacaccccg gaaggccaga aagaaatcga gatagaaggc
    13441 gactcggccg acatcatggc ggatacggtt catttgatga atgtcatggc caaggcttat
    13501 gcggaagagg ttcctgtgac tgtgacggcg gacaaggggc gatacgataa aaaggaaaat
    13561 ctcattcaca ttgagcagaa cgtggtggcg accaccgagg acggcacgcg gcttttaacc
    13621 gaagagctcg acatgcatcc cgaccggcat gtcattgaaa atgaagt
//
LOCUS       DAOSIO010000019        12929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_4893_length_12929_cov_4.047305, whole genome shotgun sequence.
ACCESSION   DAOSIO010000019 DAOSIO010000000
VERSION     DAOSIO010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 12929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12929
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4893_length_12929_cov_4.047305"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /gene="rsfS"
                     /locus_tag="PK590_01540"
     CDS             <1..278
                     /gene="rsfS"
                     /locus_tag="PK590_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOG23311.1"
                     /translation="LTHYFIVTHGNSAPHVRAISEHIMVEMKKRKMPVFHVEGLQDAK
                     WVLLDFGSVIVHVFYKETREFYSIERLWGEGKLLLKRDEVDEKQDAE"
     gene            357..2012
                     /gene="argS"
                     /locus_tag="PK590_01545"
     CDS             357..2012
                     /gene="argS"
                     /locus_tag="PK590_01545"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOG23312.1"
                     /translation="MKFKIETTLATIVEKLFTARGIEKPAGFSCELHPPRGAGRGDLS
                     TNVAFKLSKILKKKPAEIAGMIIAAFEELGDSPVERMEIAGGGFINFFFRKKDLGSVI
                     VAIHQADRRYGSSDYGKGKKVIVEFVSANPTGPMTIAHGRQAAVGDSLCRILKAAGYD
                     VHAEYYLNDAGRQMNLLARSVWARYHEILGHEMALPEDGYKGEYVRQIARDIIEKEKK
                     DNLLQGPEDKAIEYCGAYAVETIMSWIKTDFAKMRVHFDHYFSEKTLYEKKSVEHALE
                     VLRQKGFLFENEGALWFRSTDFGDDKDRVVKKSTGEYTYLAPDIAYHLSKYERGYEVL
                     VNFWGPDHHGYIPRLRAACQALGYPPEAIHIGIVQLTTLFREGQPVRMSTRAGEFVTL
                     RELYDEVGVDATRFFFTMRRQESPLDFDLDLAKERSQENPVYYLQYAHARIASLLKFA
                     EEKVTTEVDLGLLSSREEGELIGCLSQFPEMIVAASRLLEPYRLADYLRQVAACFHKF
                     YSFHRIVTEDKDLTKARLLLSDATRVVLRNGLELLGISQPESM"
     gene            2121..3086
                     /gene="miaA"
                     /locus_tag="PK590_01550"
     CDS             2121..3086
                     /gene="miaA"
                     /locus_tag="PK590_01550"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOG23313.1"
                     /translation="MLTSPVVLLIVGPTASGKTSLSIELAKRLGGEIISADSMLVYKG
                     MDIGTAKPSLKERLKIPHHGIDLVEPTRKFSVFQYRQFALKTIRDVVSRGKVPIVVGG
                     TGFYVRALLEGLSPLPGADAKIRKQLERELKQEGPERLYKRLLKIDPKRARDIGPHNV
                     RRVMRALEIYLVSGRKPSLPAAPSPGLRELGYCPVVIGLNRDRAFLYEEINRRVERMF
                     RKGLLAEIKKLSKKRLSETARQGVGYKEILADALKTSGLAAEWERRARTQGRDRIKRA
                     TRRFAKRQWTWFKKEKGIRWVWWPEGLPTQAVAEFVQNSMLGPLS"
     gene            3171..4250
                     /gene="hflX"
                     /locus_tag="PK590_01555"
     CDS             3171..4250
                     /gene="hflX"
                     /locus_tag="PK590_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOG23314.1"
                     /translation="MADRVFLVIARHSRMNAADTAAMLAEMKELTLSANGTLVGETRA
                     NIAEPSPSHYIRGGKLEELRVRTLETRANVVIFSVDLSAVQARNIENALGGVRVVDRT
                     GLILDIFARRAVSRDGKLQVELAQLQYLLPRLTGKGVIMSRLGGGIGTRGPGEQKLEI
                     DRRKIRNRIEHIKGQIGKLSRHRALVRASRRQKGLRQVVIVGYTNAGKSTLLNALTGA
                     DAYVENRLFATLDPKTRIFNDAAGDPVLFTDTVGFIRDLPHGLVKAFHATLEEATEAD
                     LILMVVDASDDQALKHKGVVEQVLKEIGAEARPRVMALNKVDLLNDQAKARIADQFPE
                     ALLVSASQKSGLADLLKKIRETLFV"
     gene            4388..7264
                     /locus_tag="PK590_01560"
     CDS             4388..7264
                     /locus_tag="PK590_01560"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HOG23315.1"
                     /translation="METVMESRASIELFKAGQGPWLDTISRRLLKSGQLKKWIEEDGL
                     LGVTSNPSIFQQAITQGEGYEQDIRKMLARGASTLEIYDSLTIADIRETCNLFLPVFE
                     GTRREHGFVSLEVLPCLAANAEATIREAQRLFSAVHCPNLMIKVPATPAGILAVEELI
                     ALGINVNVTLIFSQANYREVARAYIAGLTRFVAKGGDPANVHSVASVFVSRIDTEIDK
                     QLDLKLAGERDRVKRMALEELKGKAAIANTKLIYQDFKEIFSSDEFRLLRDRGAFVQK
                     VLWGSTSVKNPSYPDLVYVENLVGHDSVNTLPQATWEALLDHGEIRANSIEENIDESR
                     DIVSRLKKFGIDIHEVCEKLQRDGVKAFVVAFETLMSVIEKKRFENLKCAGAVKTTYA
                     FPEKTQKAIEVAIRDFEKKDLHARWLAKDPTLWTNDPAHHKVILNRLGWVEIAEKVTG
                     KLYELDLLQEKIASEKVRDIVLLGMGGSSLAAEVMASILKKPSHRSPSAAKPRFHLLD
                     TTDPLSISRVNDVIRYDKTIFIVGSKSGSTIETRSQYQFFFHAVKRFYRGDEAKAASR
                     FVIVTDEGSPFAQMGKAMNLAGLFLNPSDIGGRYSALSLFGLVPAASLGMNVRKVLKD
                     AQRALSNMRASRDLKKNRGIYLGIVLGILALQGKDKLTFLTSPSLASFADWAEQLIAE
                     STGKEGKGITPVVGEPFLGLEKYGSNRVFVLLRQKGENEKGWADRSRALKAKGFPVID
                     CLWDDGINVGGEFLVWEIATSIAGVVLRINPFDEPNVKESKDITGRILQKIQKKKALP
                     KPAMSLKASSARLSTVEKNLLGKFFGKLKPNGYLSLLAYIDRTVASKKDLQAIQRLLA
                     AALRVPALSGFGPRYLHSIGQLYKGGPRNGSFIEFYVRDTKDLKIPGECYGFSQLKTA
                     QAMGDYLATSNKGLPVLVVDLGRDPNRGLRTFRSKLEAYLKSAV"
     gene            7364..8170
                     /locus_tag="PK590_01565"
     CDS             7364..8170
                     /locus_tag="PK590_01565"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOG23316.1"
                     /translation="MFQNRKSPLHNLRFLFFAVFLGTGVFFSGCASTPPPPPMPNFRP
                     ASVMTVNLPAPTTGTKRDIYHVVGPYESLWRIAIVYGKDMDSIMRDNQISDATKIKKG
                     QKLLIRNTYGPRPNIPLYPTTRWTHIVIHHTATDVGNAYTIDSAHVNRGWERGMGYDF
                     LIDNGTHGKSLGQIEVGPRWLKQIDGAHVKEADWNKKAIGIALMGNYSENPMPAPMMD
                     NLVFLVATLKNFYRIPEQNIVGHRDVPGAATECPGKLFPWNEFKRRVRMY"
     gene            8421..8633
                     /locus_tag="PK590_01570"
     CDS             8421..8633
                     /locus_tag="PK590_01570"
                     /inference="COORDINATES: protein motif:HMM:NF025005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HOG23317.1"
                     /translation="MINNMVLSTEIEWIGYEHRRSMLQVEFIAGPVYQYDNVPEKIYS
                     DFLTAPSHGRFFESNIKGKFSCRKVR"
     gene            8859..10439
                     /locus_tag="PK590_01575"
     CDS             8859..10439
                     /locus_tag="PK590_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium symporter"
                     /protein_id="HOG23318.1"
                     /translation="MTQFTHTTKPRERWNTRLGLILAMAANAVGLGNFLRFPVQCANH
                     GGGAFLIPYFIAVILLGLPLMWIEWGIGRFGGKYGHGTTPGMFHYLWRHPIAKYIGAI
                     GIAVPFLLVIYYNYIVSWTLAYSVFSFTGKYAGLTSFEGMSEFLAAYQGKVSNAHFPT
                     IATAFLFFLLTHGIMMWVVSHGVTRGIERLVRIALPLIIVFGVILVVRVLFIGTPNPA
                     HPENNIANGFGFLWNPNFSVLKDSKVWLAATGQVFFTLSVGVGAIQTYASYLKPKDDV
                     VLSSIATCATNEFCEVILGASIAIPVTFAFFGLAQTRLIAAGGAFDLGFFAMPIIFQQ
                     MFLGQFFGAIWFFLLFLAGISSTVAMVQPVMAFLQEEFRMTRKSASWVVSVMVLFFSF
                     PVIFFLKHGFLNELDFWVGTFGLVVFAIIEVLIFLWVFGERRAWKEINSGSEIHIPRV
                     VIRIIKYVTLAYLVVLLVFWFAQDGISFLLMKNVPREDFPYVWFARFMMLAVSALMIF
                     LVHLAWQRKRRIRQRMPD"
     gene            10749..>12929
                     /locus_tag="PK590_01580"
     CDS             10749..>12929
                     /locus_tag="PK590_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23319.1"
                     /translation="MSDKTVKNKSIRFRITAAIVLVCFITTSVSSPVSANASPAGSAV
                     LPPVSLSELTIPDELGTIVRSVGVQDGAAADPDKTNVRDSSRRTVILIQDAHAVNDAQ
                     DKIQKIIAHLQTHHYADMLLVEGAQGRLDPTILRNYPDENAKQRVVREYLERAELAGV
                     ESAAVLNDTLGHFEGAEDWALYSENFNAYHRAQAEKESLLDILSRHERKLETENTGRI
                     NSELQDFLKRWTGFRSGSVSFLDFLISLQEFSNLAKEEEDGFSELKRILDALGYEKSS
                     QSAELEPTVKKMASEFKRKFANVMDVRDQANFYMHYQKFLTGQSGAGEMLTCLLRLGR
                     KLGQSPKLSPEMRRLLGHTQTLTGIKGNRLFEDLNRFLGMVAMSLAKDSDDVSAIRRY
                     EELFVLEDLVKLELTHHSLFSLMKSEARGAKFETDSRSSDLRTSDKTGILNLDDFDLG
                     FLPDLGLSVPDFEKKLQPALDFYRAALERDRVFYDRIKAAPESARAIVLIAGGFHTSG
                     IERLLRENRDSYAVVTPRIGSLTGSENYLRVMNNDLSWQEYIGATYYDGLIRHAIRKL
                     TEDLSESEFRQTAKRWRDNLIRDLASQNRITDFGQYSQYLGDLNRIYAERFAKDLKPL
                     TKEDILGVIKRELEKFKSDVLVSLWKEVEDGPQGATAGFSGLEERKEPSVTKPSDLAF
                     DIPSSKPLMLSPALKLAPENLALTAPRSEVRQQTVAARDVSEVLR"
BASE COUNT         3038 a         3033 c         3624 g         3234 t
ORIGIN      
        1 gcctgaccca ctattttatc gtgactcacg gaaatagcgc cccccacgtt cgcgcgattt
       61 ccgagcacat catggtcgag atgaaaaaac ggaagatgcc tgtttttcac gtggagggcc
      121 tgcaagacgc gaaatgggtc ctgctcgatt ttggttcggt gattgtccac gtcttctaca
      181 aggaaacccg cgaattttat tcgatcgagc gtctttgggg ggagggaaaa cttcttttaa
      241 agcgggacga ggttgacgaa aaacaggacg cggaatgatg ccagttcccc ccgcgtcagg
      301 tgttgctttt cggttccttt tacccctctt ttttaatggt ctgaccaggt gactgtatga
      361 aatttaaaat cgaaaccact ctcgcgacga tcgttgagaa actttttacc gccaggggga
      421 ttgaaaagcc cgccggtttt tcgtgcgaac ttcatccccc gagaggggcc ggccgggggg
      481 atctttccac gaatgtcgcc ttcaagctgt ccaagatttt gaagaaaaaa cccgccgaga
      541 tcgcggggat gattatcgcc gcgtttgagg agctggggga ttcgcccgtg gaacgaatgg
      601 agatcgcggg cgggggattt atcaattttt tctttcgcaa gaaagatctg ggttcggtga
      661 ttgtcgcgat ccaccaggct gaccgccgct acggcagttc agattatgga aagggaaaaa
      721 aggtcatagt tgaatttgtg agcgccaatc ccaccggacc gatgacgatt gcgcacggga
      781 ggcaggccgc cgtgggagat tccctgtgcc ggatcctgaa agccgccggt tatgatgttc
      841 acgccgaata ttatctgaac gatgccggcc gacagatgaa tttgctcgcg cgaagcgtct
      901 gggcccgcta ccacgagatc ctggggcatg aaatggcgct tcctgaggac ggttataagg
      961 gcgagtatgt ccgccaaatc gcccgtgaca taattgaaaa agaaaaaaaa gacaaccttc
     1021 ttcaagggcc ggaagacaag gcgattgaat attgcggcgc ctacgcggtg gaaacgatca
     1081 tgagctggat caagacggat ttcgcgaaaa tgagagtcca ttttgaccat tatttcagcg
     1141 agaaaacact ctatgaaaaa aaatccgtgg agcacgcgct tgaggtgttg cggcagaaag
     1201 ggtttctttt tgaaaacgag ggggcgcttt ggttccgttc gaccgatttc ggggatgaca
     1261 aggatcgggt ggtgaaaaaa tcgacggggg agtacaccta tctggcgccg gatattgcct
     1321 atcatctctc aaaatatgaa cggggttatg aagtgcttgt taatttttgg gggcctgatc
     1381 accatggcta cattccacgg cttagggcgg cttgtcaggc tctgggttat ccgccggaag
     1441 cgatccatat cgggattgtt cagctcacga cacttttccg ggaaggacag cccgtgcgaa
     1501 tgtcgacccg ggccggggag tttgtaaccc ttcgcgagct ttatgatgaa gtgggcgttg
     1561 atgccacccg ttttttcttt acgatgcggc gacaggaaag cccgcttgat tttgatctcg
     1621 atctcgcgaa ggagcggtct caggaaaatc cggtttatta ccttcagtac gcccacgccc
     1681 gtatcgccag ccttctgaag ttcgcggagg aaaaggtcac gacggaagtg gatctgggac
     1741 tgctttcctc cagggaggaa ggtgaattga tcggttgtct atcacagttc cccgagatga
     1801 tcgtggcggc gagccggctc ctcgaaccct accggctcgc ggattatttg aggcaagtgg
     1861 cggcgtgttt tcataagttt tattcttttc accggattgt gaccgaggac aaggatctca
     1921 cgaaagcgcg tttgcttttg tccgacgcga cacgcgttgt gcttcggaac ggtctcgaat
     1981 tacttggcat ctcacaaccg gaatcgatgt aggattggga gggtggggct tggcagacag
     2041 aacaacgttt ttcccacccc tcgaacactt tacccttatt ggcgcggatg ataaccccgt
     2101 acgcccgtga atgaaaatac atgctgactt ctcccgttgt cttgttgatt gtcggtccga
     2161 ccgcttcagg aaaaacttcc ctctcaattg aactcgcgaa acgtcttgga ggcgagatta
     2221 tttctgccga ttccatgctt gtttacaaag gaatggatat tggcaccgcg aagccgtcct
     2281 taaaagaacg tctcaaaatt ccgcatcacg gcattgatct tgtcgaaccg acccggaaat
     2341 tttccgtgtt tcaataccgt caattcgccc tgaaaactat ccgcgatgtt gtctcacgcg
     2401 gtaaagtccc gattgtggtg ggcgggaccg ggttttatgt cagggcgctt ttggaggggt
     2461 tgtcgccttt gcccggggct gacgcgaaaa ttcgcaaaca actggaacga gaattgaagc
     2521 aggagggacc tgagcggctc tataagcggc ttttgaagat tgaccccaag agagcccgcg
     2581 acatcgggcc gcataacgtg aggcgcgtta tgcgggcgct tgagatttat ctggtttcag
     2641 ggcgaaagcc ttccctgcca gccgctccaa gtcccggtct gcgtgaattg ggttattgcc
     2701 cggtggtcat cggacttaac cgggacagag ctttccttta tgaggagatc aatcgccggg
     2761 ttgaacgaat gtttcgaaaa gggcttttgg cggagattaa aaagctttcg aaaaaacggc
     2821 tctcggagac ggctcgtcag ggagtgggct ataaggaaat tcttgccgat gccttgaaaa
     2881 cttcgggttt ggcggcggag tgggaacgcc gggcgcgaac acagggtcgg gacaggatca
     2941 aacgagcgac gcggcggttc gcgaagcgtc aatggacctg gtttaaaaaa gaaaaaggaa
     3001 tccgttgggt ttggtggcct gaaggtctgc caacccaggc ggtagcggaa tttgttcaaa
     3061 actccatgct gggcccactg tcataacttt aattctccaa aaacggggca gggatcatga
     3121 atctgatttt tttcggaggg cgcgcggggg ttacggagtg atgactttct atggcggata
     3181 gagtgtttct tgtgattgcc aggcattcac gcatgaacgc ggcagatacg gcggccatgc
     3241 tggccgagat gaaagagttg actctctccg ctaacggaac cctggtgggg gaaacgcgcg
     3301 cgaatatcgc ggagccgagt cccagtcatt acatccgggg cgggaagctt gaagagctca
     3361 gggttcgtac cctcgaaacg cgggcgaatg tcgtcatttt cagcgtggat ctttcggcgg
     3421 tacaggctcg caacattgag aacgcgctgg ggggcgtccg ggtggttgac cggacgggat
     3481 taattctcga catttttgcc cgtcgcgcgg tttccagaga tggaaaactt caggtggagc
     3541 ttgcccagct ccagtatctc ttgccgaggc tgaccggaaa aggcgtgatt atgtcgcggc
     3601 tcgggggtgg aatcgggacc cgggggcccg gggaacagaa actcgagatt gaccgacgaa
     3661 aaatccggaa tcggattgaa catatcaaag gacagattgg gaagctttcg cggcatcggg
     3721 cgctggtcag ggccagccgc aggcaaaagg gcctgcgtca ggttgtgatt gtcgggtaca
     3781 cgaatgccgg aaaatcaacc ctcctgaacg cgttgaccgg ggccgatgcc tacgtggaga
     3841 atcgcctgtt cgccaccctc gatcccaaaa cccggatttt taatgatgcc gccggagatc
     3901 cggttctttt cacggacacg gtcggcttta tccgggatct gcctcacggg ctcgtcaaag
     3961 cctttcacgc gacccttgaa gaagcgactg aagccgacct gatcctgatg gtggtggacg
     4021 cctccgacga ccaggctctg aaacacaagg gtgtggtgga gcaagtcttg aaagaaattg
     4081 gcgcggaggc ccggcccaga gtgatggcgc tcaacaaggt ggatttattg aatgatcaag
     4141 cgaaggcccg tatcgctgat cagtttcccg aagccctgct tgtttcggcg agccaaaaat
     4201 cagggttggc ggatctgctg aaaaaaatcc gagagacgct ttttgtctga tgcccgattt
     4261 ttcgcaggct gtttcagaaa agtttcatga aagcgggata ggctatgtta taatcccgct
     4321 tgtttgaccc gcccaagatg gcttaatttc aggaaatatc caacaccact tgttgagaga
     4381 ggacattatg gagactgtga tggaatcacg cgcgagtatt gaacttttta aagcggggca
     4441 ggggccctgg cttgacacga tcagccggag gcttttgaaa tccgggcaac taaaaaaatg
     4501 gattgaggaa gacggcctcc tcggcgtgac ctcgaatcct tccatctttc agcaggctat
     4561 cacccagggt gagggttacg agcaggacat tcgaaaaatg ctcgcccggg gagcttccac
     4621 gcttgaaatt tatgattcac ttacgatcgc tgatattcga gagacctgta atctttttct
     4681 tccggttttt gagggaacac gaagggagca tgggtttgta agcctcgagg tcctcccgtg
     4741 tcttgcggcg aatgcggaag ccacgattcg tgaagcccaa cggctgtttt cggccgtcca
     4801 ttgtcccaat cttatgatca aggttccggc cacgccagca gggattctgg cggtggagga
     4861 gctgatcgct ctcgggatta acgtcaatgt aacgctgatt ttttcccagg cgaattaccg
     4921 cgaagtggcc cgggcatata ttgccggtct gacgcgattt gtggcgaaag gcggagatcc
     4981 cgcgaatgtc cattcggtgg cgagtgtttt cgtgagccgg attgatacgg agattgataa
     5041 acagctggat ctgaaacttg ccggggagcg ggaccgggtg aaacgaatgg ccctcgaaga
     5101 attaaagggc aaagccgcga tcgcgaatac caaactgatc tatcaggatt ttaaggagat
     5161 tttctcttcg gacgagttcc ggttgctccg ggatcgcggg gcatttgtcc agaaagtcct
     5221 ttggggaagt acgagtgtca aaaatccttc ctatcccgac cttgtttacg tcgaaaacct
     5281 tgtgggtcac gacagtgtca ataccctgcc acaagccaca tgggaagctc ttctggatca
     5341 tggagagatc cgcgcgaatt ctatcgagga gaatattgac gagtcgcgcg atatcgtttc
     5401 ccgtctcaag aaattcggaa tcgatatcca cgaggtatgc gagaagctac aacgggatgg
     5461 ggtcaaggcc ttcgtcgtgg cttttgagac cctgatgtcc gtgatcgaga agaaacgttt
     5521 tgaaaatctt aaatgcgccg gcgcggtcaa gactacctat gcttttcctg aaaaaacgca
     5581 aaaagccatt gaggtcgcga tcagggattt tgaaaaaaag gatctgcatg cccggtggct
     5641 cgcgaaggat ccgaccctct ggacaaatga tccggctcat cacaaggtga tcctgaaccg
     5701 gctggggtgg gtggagatcg ccgagaaggt gacggggaaa ctttatgaac tggatctttt
     5761 acaggaaaag atcgcgagcg aaaaggttcg cgatattgtg ttgcttggca tgggaggttc
     5821 cagtctggcg gccgaagtga tggcgtcgat tctcaaaaaa ccttcccatc gctcaccgtc
     5881 ggcggccaaa ccacgctttc acctgttgga caccacggat ccgctcagca tttctcgggt
     5941 caatgacgtg atccggtacg ataaaacgat atttatcgtt ggaagtaaat ccggttccac
     6001 cattgaaacc cgttcccaat atcagttttt ctttcatgcc gtcaagcggt tctaccgggg
     6061 cgacgaggca aaggccgcga gccggttcgt gatcgtgacc gatgaggggt cgccgttcgc
     6121 ccaaatgggg aaggccatga acctggcggg acttttcctg aacccctccg atataggcgg
     6181 acgttattcg gcgctttcgc tttttggact tgtgccggcg gcgtctctcg ggatgaatgt
     6241 ccggaaagtg ctcaaagacg ctcaaagggc tctctcgaat atgagggctt ccagggactt
     6301 gaaaaaaaat cgcgggattt acctcggaat tgtcctcggg attctggcgc ttcagggaaa
     6361 ggacaagttg acgtttctga cgtcaccgtc actggcttct ttcgcggatt gggcggagca
     6421 actgatcgcg gaaagtaccg gaaaagaagg taagggtatc actcctgtcg ttggagagcc
     6481 ttttctcggt ctggaaaaat acgggtcgaa ccgtgtgttt gttcttctgc ggcagaaagg
     6541 ggaaaatgaa aagggatggg cggatcgatc tcgcgcgctc aaggcgaaag gttttccggt
     6601 cattgattgt ctttgggacg atggcataaa cgttggcggg gaatttctcg tctgggaaat
     6661 cgcgaccagc attgccggcg ttgttctgcg gatcaatccg ttcgacgagc ccaatgtgaa
     6721 ggagtcgaag gatattacgg ggcgcatcct tcagaaaata cagaaaaaga aagctctgcc
     6781 gaagccggct atgtcgctta aggcatcatc ggcgcgtctt tccacggttg aaaaaaatct
     6841 gcttggcaag ttttttggca agttgaaacc caatggctat ctgtcgttgc ttgcctacat
     6901 tgaccggacg gtggcttcca aaaaggattt acaggccatt cagcggcttc tggcggcggc
     6961 attgcgtgtg ccggctctgt caggctttgg tccgcgttat ctgcattcga tcggacagct
     7021 ctataagggc ggtcccagga acgggagctt catcgagttt tacgttcgcg acacaaagga
     7081 tttgaagatt cccggtgaat gctatggatt cagccagtta aagacggcgc aggcgatggg
     7141 ggactactta gcgacgtcaa acaaggggct tccggtgctt gtggtggatc tgggacgtga
     7201 tcccaacagg gggcttcgaa cctttcgctc gaaactggag gcgtatctga agtctgccgt
     7261 gtgatttcac gcgtcgcgcc ggtttcctga aggcgcgatt aaaaataagc tcaagcaaaa
     7321 ggctaacagg gcgaaaatat agtcaaaagg atttaaacac gccatgtttc aaaaccgaaa
     7381 atctccgctc cacaatctga ggtttctttt tttcgcggtt ttcctgggga cgggtgtttt
     7441 tttctcagga tgcgcctcga cgcctcctcc gcccccaatg cccaattttc gtccggcaag
     7501 cgtcatgacg gttaacctgc cggctccaac gaccggcacc aagcgtgaca tctaccatgt
     7561 ggtcggaccc tacgagtctc tttggcggat cgcgatagtt tacgggaagg acatggactc
     7621 gatcatgcgc gataaccaga tttcggacgc gactaaaatt aaaaaagggc agaagctcct
     7681 gatccggaac acctatgggc cgcgtccgaa tattccgctc taccccacaa cgcgctggac
     7741 gcatatcgtg atccatcaca cggccaccga tgtcgggaac gcttatacga tcgattccgc
     7801 gcacgtaaac cgcggctggg agcggggaat gggatatgat tttctgattg ataacggaac
     7861 tcacgggaaa agtttaggcc aaattgaggt ggggccccga tggctgaagc agattgatgg
     7921 cgctcacgtt aaagaggcgg actggaataa aaaggcgatt ggcatcgccc tgatgggaaa
     7981 ttatagtgaa aatcccatgc ctgcgcccat gatggataac cttgtttttc tcgtggccac
     8041 tctcaaaaat ttctacagaa taccggagca gaatatcgtt ggacaccgcg atgtcccggg
     8101 ggcggcgacc gagtgtcccg gaaagctttt cccctggaac gagtttaaac ggcgggtccg
     8161 gatgtattga tcgaaaatcg aaattccgga cgaagaaaat tatttcactc accaaccaga
     8221 cacgtattcc tcgctggcct cccatcccca atcttctgaa atatcagtaa aaacaagcgc
     8281 gcgtggtcaa gatttaactt tttttgccgt aacatacaag gtccgaggct gggaagaaaa
     8341 tttgatggta acgctgttga gataacctcg gatttaaaaa aaacagttaa atcatttcta
     8401 atctaaaagg tggagggact atgatcaaca acatggtatt atcgactgaa atcgaatgga
     8461 tcggatatga gcatcgtcga agcatgttgc aggtggaatt tattgccggg ccagtctatc
     8521 agtatgacaa tgtccccgag aagatctact ctgatttttt gacggctccg tctcacggaa
     8581 gatttttcga aagtaatatt aagggaaagt tttcctgccg taaagtacgc taattcaccg
     8641 gttttatttt ttacttgagg gtttttgcgc gatgtttttg gcgttatttc tgtttatcca
     8701 tccggtgaat ccgtctcttg ccccgcgctc gctgaccgag gatactctcg tcattcaaag
     8761 ttgaggattg ccccaacacg gaatcctgaa cggctccgtc ctctccttgc aattcgggaa
     8821 ggcttacgta gaataggcct tttgcggatg ggaggattat gacacaattc acacatacca
     8881 cgaagccgcg ggaacgatgg aacacccgat tgggactgat cctggccatg gcggcgaacg
     8941 cggttggtct tggcaatttt cttcgttttc ccgttcaatg cgcaaaccat ggcggtggag
     9001 cttttctcat tccttacttt atcgcggtta ttcttctcgg acttcccctc atgtggatcg
     9061 agtggggaat cgggcgtttc ggaggcaagt acgggcatgg gaccactccg gggatgtttc
     9121 actacttgtg gcgtcatccc atcgccaagt acatcggcgc gatcgggatc gcggtaccgt
     9181 ttctgctggt gatctactac aactacattg tgtcctggac cttggcctat agcgtgtttt
     9241 ctttcacggg gaagtacgcg gggctgacga gttttgaggg gatgtcggaa tttctcgcgg
     9301 cctatcaggg aaaagtctcc aacgctcatt tcccgacgat tgcgacggcg tttctttttt
     9361 ttctgttgac gcatggcatt atgatgtggg tggtttcgca tggggtgacc cgggggattg
     9421 agcgactggt ccggattgcc ttgcctctca tcattgtctt tggagtgatc cttgtggttc
     9481 gggtgctgtt cattggcacg ccgaatccag cccacccgga aaacaacatc gcgaacgggt
     9541 ttggttttct ctggaatccg aatttttcag ttttgaagga cagtaaggtc tggctggccg
     9601 ccacagggca ggtttttttc acattaagcg tcggtgtcgg ggcgatccag acttacgcga
     9661 gctatctcaa acccaaggat gatgttgttc tctcaagtat cgcgacttgc gccacgaatg
     9721 agttctgtga agttattttg ggggcgtcga tcgccatccc ggtgacgttt gcctttttcg
     9781 gtctcgctca gacgcgcttg atcgcggcag ggggggcttt tgatcttggt tttttcgcga
     9841 tgccgattat tttccagcaa atgttcctgg ggcagttttt tggggccatc tggttttttc
     9901 tcctgtttct tgcggggatt tcctcgacgg tcgccatggt tcagcccgtc atggcatttt
     9961 tgcaggagga atttcgaatg acgcgaaaat ccgcatcatg ggtggttagc gtcatggttt
    10021 tgtttttctc gttccccgtg attttttttc tgaaacatgg ttttctgaat gagcttgatt
    10081 tctgggtggg aactttcggg ttagtggttt ttgccattat tgaggtgctg atttttctct
    10141 gggtgtttgg cgagcgaaga gcctggaaag aaattaactc ggggtcggag atccatatac
    10201 cgcgtgtcgt gatccgaatt ataaaatatg tgacgctggc ctatctggtt gttcttttgg
    10261 tgttctggtt tgcccaggac ggaatttctt ttttgctgat gaaaaatgtg ccgcgagaag
    10321 attttcctta cgtgtggttc gcgagattta tgatgctggc ggtgtccgcg ctgatgattt
    10381 ttctggtgca cctggcgtgg caacgtaagc gtcgcatccg gcaaagaatg cccgactgat
    10441 tttttcacgc gtcttctgcc tttccgcaac gtagatttga atccctgaat tttccgaaat
    10501 ggtagtcttg tctaatatct atagcaaaaa atagcaaaat atatcataaa gatataataa
    10561 gcaaaagata ttaaaagata ttatatggac atacttgaaa taactttatt atgatggcat
    10621 gctttaagta gatgaaaaca atcaagatgt gaacattaga ttagctgatt tccttcgact
    10681 gtcttgacgt ggaggagcgt taaggcgctt caaactttta ataactttta actaagacgg
    10741 cattttttat gagcgacaaa actgtcaaaa acaaaagtat ccggtttcgg attactgccg
    10801 ctatagttct ggtttgtttt ataaccacga gcgtgtcgtc acctgtgtcc gcgaatgctt
    10861 cgcccgcagg ttccgcggtt ctgcctcctg tgtccctttc cgagttaacc attccggatg
    10921 agctgggaac cattgtgaga tcggttgggg tacaagatgg ggcggcggct gatccggaca
    10981 aaaccaatgt ccgagattct tccaggcgaa ccgttatcct gatacaagac gcccatgccg
    11041 tgaatgatgc gcaggataaa attcaaaaaa taatcgcgca tctgcaaacc catcactatg
    11101 ccgacatgct ccttgtggaa ggggcgcaag gccgtctgga tcccacgata ctccgaaatt
    11161 atcccgatga aaatgcaaaa cagagagtcg tcagggaata tctcgagcgg gcggaacttg
    11221 ccggcgtgga atctgccgcc gtattaaatg acacgcttgg ccatttcgag ggagcggaag
    11281 actgggccct ttattcggaa aacttcaacg cctatcaccg ggcgcaggcg gaaaaagaaa
    11341 gtctcctgga catcctgtcg cgccatgagc gcaagctcga aacggaaaat accggtcgaa
    11401 ttaattcgga gttgcaggat tttcttaaac ggtggacggg gtttcggtcc ggctctgttt
    11461 cctttcttga tttccttatt tccttacagg agttttcaaa tctggcgaag gaggaggagg
    11521 atggattctc ggagcttaaa agaattttgg acgcgctggg ttatgagaaa tccagccaat
    11581 cggccgagct cgaacccacg gtaaaaaaaa tggcctccga attcaaaagg aaatttgcga
    11641 acgtgatgga tgttcgcgac caggcgaact tttacatgca ctatcagaaa ttcctgacgg
    11701 ggcaatcggg agcgggtgaa atgttgactt gtcttctacg gctgggacgg aagttggggc
    11761 agtcgcccaa gctcagtccg gaaatgcgtc gtcttctggg gcacacgcag actctgaccg
    11821 ggattaaggg aaacagactg ttcgaggatc tgaaccggtt tctcgggatg gttgccatga
    11881 gcctcgctaa agactctgac gacgtgagcg cgatcaggcg ctatgaagaa ttgttcgttc
    11941 ttgaagatct tgtgaaactg gagttgaccc accactcgtt attctcactg atgaaatctg
    12001 aggcgcgcgg cgcgaaattt gaaacggatt cccgaagctc cgatttacgg acttccgaca
    12061 aaaccgggat cctgaattta gatgattttg atttgggatt tcttccggat ttgggattga
    12121 gcgttccgga ttttgaaaaa aaattacagc cggcccttga tttttaccgg gccgccctgg
    12181 aacgtgaccg ggtattttac gatcgcatta aagcggcgcc cgaaagcgcg cgcgcgattg
    12241 tcctgattgc cggaggtttt cataccagcg ggattgagcg actgcttcgc gaaaaccgcg
    12301 actcgtacgc ggtcgtgacc cccaggatcg gatccctcac gggctcggaa aattatcttc
    12361 gcgtcatgaa taacgatctt tcctggcagg aatatattgg cgccacctac tacgatggat
    12421 tgatccgtca cgcgattcgc aaattgacgg aagacctcag tgagtctgag ttccggcaaa
    12481 cggccaagcg ttggcgcgac aatcttatcc gggatcttgc ctctcaaaac aggatcacgg
    12541 acttcgggca atatagccaa tatcttggtg atttaaaccg gatttatgcc gaacgtttcg
    12601 ccaaagatct taaacctctg accaaggaag atattctggg ggtgatcaaa agagagttag
    12661 agaagtttaa atcggatgtg ctggtttcac tttggaaaga agtcgaagat ggtccgcaag
    12721 gcgccaccgc cggattttcg ggactggagg aacgaaaaga accttctgtt acaaagccct
    12781 cggatctcgc gtttgatatt cccagttcca agccgttgat gcttagcccg gctctaaagc
    12841 ttgctcccga gaatctggca ttaacagcgc ctcgcagtga agttcgccag cagacggtag
    12901 cggctagaga cgtttctgaa gtcttgagg
//
LOCUS       DAOSIO010000020        12865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_4926_length_12865_cov_4.390398, whole genome shotgun sequence.
ACCESSION   DAOSIO010000020 DAOSIO010000000
VERSION     DAOSIO010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 12865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12865
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4926_length_12865_cov_4.390398"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /gene="tig"
                     /locus_tag="PK590_01585"
     CDS             <1..1362
                     /gene="tig"
                     /locus_tag="PK590_01585"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOG23320.1"
                     /translation="VVFRMDFFKKKKDAQDAEPQDDSHSDVATGAPFKVKLRDGKVCE
                     KILTIEVGKDKILNEYENYYAAIAPKAKVPGFRPGKVPRDVIEMHYEKNASDAVLEHL
                     LSESLPYAIREKGLSPLATPELHDIQFSKEKLSFRAHIEIRPKVKLSKVEGLSAKREK
                     VDVKPEEVEKSIENLREIHCQYKAVERPAAMGDFVIADYVCVIENKEFEKRKDDWIEL
                     KENEYLKGFSAQLVGVKAGDTKDVHLTMPESMQNKNMAGKPAIFRMTVKEVKEKILPA
                     VDEELAKQAGDYKDLADLREKMKQDLVKRKEDESDAAFEKALFDELLKHNKLDIPQGL
                     LARRTEHLIDQSKQRFISQGGTEELFEQEKEKIRKEFEDEAKRQIHLAFLLDEIAEAR
                     QIKVEEPDLKKKYTDLSTRYRQPAEVIEKYYQEHKEALEALRDQVRNEKVIEFIKANA
                     KQS"
     gene            1443..2039
                     /gene="clpP"
                     /locus_tag="PK590_01590"
     CDS             1443..2039
                     /gene="clpP"
                     /locus_tag="PK590_01590"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOG23321.1"
                     /translation="MSYLVPMVIETTGRGERAYDIYSRLLKDRIVFVGTPIDDLIANM
                     IIAQILFLNMEDPSKDINMYINSPGGSVTAGLAIYDTMQFVKCDVATYCIGQAASMGA
                     FLLAAGTKGKRYVLPNARVMIHQPWGGAQGTASDIHIQATEILKLKEQLNRLLAHHTD
                     KSKEQIEKDTDRDYFMSAEEARSYGIVDQVVTSKTEIK"
     gene            2039..3157
                     /locus_tag="PK590_01595"
     CDS             2039..3157
                     /locus_tag="PK590_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOG23322.1"
                     /translation="MIQPAILLTPGPTPVPESIRQLMAEPIFHHRTPRYRKIFGDVSQ
                     RLKKVFLTQNPVYTFAGSGTLAMEAALVNFHSAGDKVLVVENGKFAERFTAIAKAFGL
                     SPIGLKVPYGEAPAPRQIEEMLAKDKDIKAVCVQLCETSTAVLSDIKAIGEIVSRTEA
                     LLIVDAISGLGADRLETDLWKVDLVVSGSQKALMLPPGLAFLSVSPKAKARMATAKLP
                     KFYYDLKHYEKSMANDDTPWTPALTLVLGLQKSLDMIESEGIDAVFKRCSENAQYTRE
                     TLQKMGLKLFSKAPSSTVTAACLPDGVDGEKLCKIMRDEKGVVVAGGQGEQMKGKIVR
                     ICHMGAIGREDLETGLKVLAETLKEMKSSSGSCCCSCQ"
     gene            3225..4820
                     /gene="serA"
                     /locus_tag="PK590_01600"
     CDS             3225..4820
                     /gene="serA"
                     /locus_tag="PK590_01600"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOG23323.1"
                     /translation="MTMMKVLVSDSLGQGGMDVLRREKNLKVDEKVGLKPEELKAIIG
                     DYDAILVRSATKVTKEIIEAGKKLRVIGRAGVGVDNVDLEAATHRGIIVMNTPEGNTI
                     STAELSFSMLMAVARNLPQADAHVKKGEWKRNKFKGSELNGKTLGIVGFGRIGREVAK
                     RANAFNMKIMVFDPFISKESVKEWPVEFVDVPTLLGNSDFLTFHTPLTAETRNLLNRE
                     TFKLCKKGMRIVNCARGGIVDETALAEALRSGQIAGAAFDVFSSEPPPADHPLLKLPQ
                     FICTPHLGAVTNEAQENVALDVAVQVIDALNDRAIKNAVNMPNLDPDTLKGLKPWLVL
                     AEKIGSLYPQLFDGSIKKVEVRYGGDPTRFKTAPLTVAILKGILTPICGDTVNFVSAP
                     NIAKERGINVSEGTSDATIDFSSFIDVEVLQGKEKYRIMGTLFNKEPRIVKINDVLID
                     VVPEGWVLFIRNEDLPGVVGSIGTILGKNGVNIAEMSLGRVGKGKKAFAMTAINIDSE
                     VPAKVVAQIKKAKPVIDVRVVKL"
     gene            4902..6176
                     /locus_tag="PK590_01605"
     CDS             4902..6176
                     /locus_tag="PK590_01605"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOG23324.1"
                     /translation="MNCILIGAQWGDEGKGKVIDILAAKSDVVVRYQGGPNAGHTIKV
                     KGRKFVLHLIPSGVLYPKKVCVIGNGVVIDPESLFEEIEMLDRNGIRIRNRFFISDQA
                     HLIFPYHRLMDQLREASLKKGKAIGTTKRGIGPCYADKMARVGIRICDLRSMDYLRQR
                     LSDVVKDRNAVLTKVYGHRPISFAKTFAYCQKIRGRLLELAIDTPRYLYDAVRARKKI
                     LFEGAQGTLLDVDHGTYPFVTSSNATVGGAITGSGIAPTFIDRIIGVVKAYTTRVGEG
                     PFPTQFPPDLMEIVQKKGEEFGSTTGRARRCGWFDAVIAEHAVRINGLDEMAIMKLDV
                     LSGMKELKIAIAYKVNGKMTRDYPHAVNEFSSAKPVYETLPGWDEDITGCKKWNDLPL
                     NARRYLKRIEQLTGTPIKIVSVGSQRHQTIFL"
     gene            6191..6919
                     /locus_tag="PK590_01610"
     CDS             6191..6919
                     /locus_tag="PK590_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOG23325.1"
                     /translation="MLNEIPKHVAVIMDGNGRWAKSKGFPRIKGHEEGVRRVEEIVKV
                     AAQKGIRYLTLYTFSKENWRRPKEEVNFLMGLLSRYLDEKINTLIEDNVVLNVIGEMS
                     DLPETIQKKLRDARKNSERNTGLTLTLALSYSSRAEILRAIKKIADRVKKQDLDIAGI
                     DESLVNANLDTAGIPDPELLIRTSGEMRISNFLLWQISYAELYVTPKFWPEFTSEEFE
                     KAIDEYRRRERRFGATEPARGLPS"
     gene            6996..7811
                     /locus_tag="PK590_01615"
     CDS             6996..7811
                     /locus_tag="PK590_01615"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOG23326.1"
                     /translation="MLNKVTIGTLGKRLLMSLIMVPAVVATIFYAPHWLFFLMVVGFI
                     MVALNEFFSIAENKGLIIHRGFGLCFGALLPFAAYASLQLPLVLAACVALFIFLFNRR
                     SPDRTITSVAVSVFGIVYVAWFFSYLIKLRLLPNGAEWVFYTILIVKGGDAGAYFIGR
                     KFGKNKLLPLVSPNKSVEGAIGQMVTTLALSVGAVFFIDVSWYHFVFLGIGVGGLAVF
                     GDLAESLIKRDAGVKDSGQMPGLGGMLDVVDSLLFTVPFVYFYLTFVLGLERI"
     gene            7808..8962
                     /locus_tag="PK590_01620"
     CDS             7808..8962
                     /locus_tag="PK590_01620"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOG23327.1"
                     /translation="MSGVKRLTILGSTGSVGENTLRIVRIHPDRFRVEALAAKKSIAR
                     LYEQAVEFRPKVVCVYDESLRDKTWVAKFKKLGVKLVSGDSGLEEVSTIPSVREVICA
                     MVGAVGLQPIFSAVRAGKIIAVANKEPLVMAGSLLLKEAAKRGVAVLPIDSEHSALWQ
                     CLENQHPDFVKKLILTSSGGPFRNFRGSLANVTRAQALNHPKWKMGPKITIDSATLMN
                     KGLEVIEAANLFRVEAKKIEVLIHPEAIVHALVELADGSHLAHLGVADMRLPIQYAMS
                     YPERLDNHLPNLDLTRLRGLHFEKPDTRRFPCLKLGYEASKIGGTMPAVLNAANEIAV
                     QAFLDQKAGFLDIPKTIEKTMTRHRLVREPGLEDILESDLWARQIAREFI"
     gene            9034..10155
                     /gene="rseP"
                     /locus_tag="PK590_01625"
     CDS             9034..10155
                     /gene="rseP"
                     /locus_tag="PK590_01625"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOG23328.1"
                     /translation="MQNLLSVFVNVLPAIVVFGILIIVHEWGHFIACRLRGVKVEKFS
                     IGFGPELFGWRSRETHFVVSAFPLGGFVKPAGEDTLSVGAEGAKPGDFLAASVGSRMM
                     IVIAGVIMNFFLAFVIFSVVYMIGRPLPGTVIGGFIQGYPAEASGLETKDKILKVDGT
                     PVFTWKDVLETLDRSRGESLSIEVSRGGKVVSFSLSPKIEETRDIFGKKVTLKRVGIT
                     PDPEVVVSERYGFFQSIARGGEAVLFHTTLTYKAIYYICARQLSPKNLMGPLGIIQVS
                     GQAAKAGVASLLLLTAILSVSLAVINLFPIPALDGGHLVFLLIEALFRKPVPAPIQEK
                     VTNAGFFALMGLMVLVFYNDIVNLQILDKLKNLLHISGS"
     gene            10194..11879
                     /locus_tag="PK590_01630"
     CDS             10194..11879
                     /locus_tag="PK590_01630"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003385849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOG23329.1"
                     /translation="MRWTQTLIPSLRNVPKDAEALSHQLALRAGLIRQLASGVYSYLP
                     LGFRVLHKVIGIIREEMNKAGASEVLLPALHPAEIWKKSGRYETLGEDKIAFKNRAGH
                     EFVLGPTHEEVITDLVGTNIKSFRDLPLTLYQIQTKFRDEARPRFGVIRTKEFIMKDA
                     YSFDRDEAGLDSHYQKMRAAYHNIFNRCGLKFQVVSADTGMMGGKMSQEFMVICPYGE
                     DRVASSESGYLASQDIAERARPELSVSQPPLEKPEPFDTPNLRTIDEISHRFKIAPSR
                     LIKTLIFIADDRPVAALVTGDSEVNEGKLRKLIGAKTIRQAEAHEIREATGAPVGFAG
                     PVGLKIPMYADWDVTAVTDGVTGANEKDKHLRHVAYGRDFTPVAAGDIRFVKDGDLAP
                     DGKGRLTLTTSMEIGHIFKLGTRYSESIGATFLDEKGERKPAIMGCYGIGVNRILAAT
                     IEQHHDEKGIKWPSEIAPFQVEIITINQADPKITTAADRLYDELNAAGVEVLYDDRDE
                     RAGVKFNDADLIGIPRQVVIGERNLAQGKVEIKDRQTGLTKMMDLCSVVSSLL"
     gene            11951..>12865
                     /gene="dinB"
                     /locus_tag="PK590_01635"
     CDS             11951..>12865
                     /gene="dinB"
                     /locus_tag="PK590_01635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOG23330.1"
                     /translation="MNWPRKFIHIDMDAFFAAVEQRDRPELRGKPVIVGGDPQSRSVV
                     STCSYEARRFGIHSAMPSSRARRLCPQAIFILPDFEKYSAVSKTVMSILRNYTPLVEQ
                     VSLDEAYLDVMENKLRIDDPVGLAKLIKQHIFAATRLTASAGIATAMFLAKIASDFSK
                     PDGLTVIPPREEVVFLKNLPVRKLPGVGPKTEAVLHRIGVRTCGDLREMSPQSLRGHF
                     GKWGNHLSQMANGTEEREVVPFWEPTQVSKEETFEKDILRVDWLKAKLGKLSRQVFTE
                     LVYKGKSGRTIVLKVKYHDFEQMTRSRTL"
BASE COUNT         3082 a         3127 c         3719 g         2937 t
ORIGIN      
        1 gtggtgttta ggatggactt ttttaaaaag aaaaaagacg cgcaagacgc ggaacctcag
       61 gatgattccc acagcgatgt tgccacaggc gctcccttca aggtgaaact ccgggacggc
      121 aaggtttgcg agaaaatcct tactattgaa gtcggcaaag acaaaattct gaatgagtac
      181 gagaattatt acgccgccat cgcgcccaaa gctaaagtgc ctggtttcag acctggaaaa
      241 gtgccgcgtg acgtcatcga aatgcattat gaaaaaaacg cctccgacgc ggtgcttgaa
      301 catctgcttt cagaatccct gccttacgct attcgggaaa aagggttgag cccgctggcg
      361 acgcccgagc ttcatgacat tcagttttcg aaagaaaagc tgagtttcag ggcccatatt
      421 gaaattcgtc ccaaagtaaa gttgtccaaa gtggaagggc ttagcgcgaa gcgggaaaaa
      481 gtggacgtga aaccggaaga agtggaaaaa tcgatagaaa accttcggga aatccattgc
      541 cagtacaagg cggttgaaag accggcggcc atgggtgatt ttgtgatcgc cgattatgtt
      601 tgcgttatcg aaaacaagga atttgaaaaa cgcaaggacg actggatcga gctcaaggaa
      661 aatgagtacc ttaagggatt ttccgcccag cttgttggcg tgaaagcggg ggataccaaa
      721 gatgttcatc tgacgatgcc cgagagcatg cagaacaaga acatggccgg aaagcccgcg
      781 attttcagaa tgaccgtcaa agaggtcaaa gagaaaatac ttcccgcggt agatgaagag
      841 ctggccaaac aggccggaga ctacaaggat ctcgcggacc ttcgggaaaa aatgaagcag
      901 gatcttgtca aacgcaagga agacgaatcg gacgcggcct ttgagaaggc cctttttgac
      961 gagcttctga aacataacaa gctggacatt ccccagggat tgttggctcg gcggaccgaa
     1021 cacttgatcg atcaatcgaa acagcgcttt atttcccagg gtggaacgga agagcttttt
     1081 gagcaggaga aggaaaagat ccggaaggaa tttgaagatg aagccaagcg gcaaattcac
     1141 ctggcttttt tgctggatga aattgccgag gcccggcaaa tcaaggttga agaaccggat
     1201 ctaaagaaaa aatacacgga tctttcaacc cgctatcgtc agccggcgga agttatcgag
     1261 aaatattacc aggagcataa agaagccctg gaagccctgc gggaccaggt tcgaaacgaa
     1321 aaagtaatcg aatttatcaa ggctaatgcc aaacaatctt gacgtttagc acgtggcgtc
     1381 tggcccgcgc gagaggggcg cggcaggcgt tgtgagctca tcgccaagag cggaggttga
     1441 aaatgagtta tctagttccg atggtgattg aaacgaccgg gagaggcgaa cgggcctacg
     1501 atatctactc ccgccttctc aaggatcgga ttgtttttgt ggggactccg attgatgacc
     1561 tgattgccaa tatgattatc gcgcagatcc ttttcctgaa tatggaagat ccttccaaag
     1621 acatcaacat gtatatcaac tcgcccggtg gatcggtgac cgcgggattg gctatttacg
     1681 atacgatgca gtttgtcaaa tgtgacgtgg ccacgtactg catcgggcag gcggccagca
     1741 tgggggcctt tttgctcgcg gccgggacga aggggaaacg ttatgtcctg ccgaatgccc
     1801 gggtgatgat acatcaaccc tggggaggcg cgcaggggac ggcgtctgac attcacatcc
     1861 aggcgactga aattttaaaa ctcaaagaac aactgaaccg gcttttggcc catcacacgg
     1921 acaagtccaa ggaacagatc gaaaaagaca ccgaccgtga ttatttcatg tcagccgaag
     1981 aggccagaag ttacggaatt gttgatcagg tagttacttc aaaaacggag ataaaataat
     2041 gatccagcca gctatcctct tgaccccggg ccccaccccg gtccccgaat ccatacggca
     2101 actgatggcg gagcccattt tccatcatcg cacgcccagg taccgcaaga ttttcgggga
     2161 tgtttcccag cgtctgaaaa aggtattcct gacgcaaaat cccgtttaca cattcgcggg
     2221 ttccgggacc ctggccatgg aggccgcgct ggtcaatttt cattcggctg gagacaaggt
     2281 tctcgtggtg gaaaatggaa agttcgccga aaggttcacc gcgatcgcga aagcgttcgg
     2341 cctcagcccg atcggattga aggttcctta cggggaagcc ccggccccta ggcagattga
     2401 ggaaatgctc gcgaaggata aagacatcaa ggccgtttgt gtccagcttt gtgaaacttc
     2461 gacggcggtg ctcagtgata tcaaggcgat cggggaaatt gtttccagga ccgaggcctt
     2521 gttaatcgtt gatgccattt cgggacttgg cgctgaccgc cttgagaccg acctgtggaa
     2581 ggtggatttg gtggtgagcg ggtcccagaa agcgttgatg ttgcccccgg ggcttgcgtt
     2641 tctgagtgtc agcccaaagg cgaaggcccg gatggcaacg gccaagcttc cgaaatttta
     2701 ttatgatctc aagcattatg aaaaatccat ggcgaatgac gacacgccat ggacgcccgc
     2761 cctgaccctt gttctcgggc ttcagaaatc tctcgatatg attgagtcgg aggggatcga
     2821 cgcggtattt aaacggtgta gcgagaacgc ccaatacacg cgtgaaacgc tccagaaaat
     2881 ggggcttaag cttttctcga aagctccatc ctcgaccgtg acggccgcct gtttgccgga
     2941 tggcgtggat ggcgagaagc tctgcaaaat catgcgtgat gaaaaaggcg ttgtcgttgc
     3001 gggtggacag ggtgagcaga tgaagggaaa gatcgtccgc atttgccaca tgggcgcgat
     3061 cgggcgcgag gatctcgaga cggggctgaa agtgctcgcg gaaaccctaa aagagatgaa
     3121 gtcttcttcg ggttcctgtt gctgttcctg tcaatgacgc gggcatgggc gggaagcgtt
     3181 tttctgtcag ggaaattttt tgaatccttc ataggagttt aacgatgaca atgatgaaag
     3241 ttcttgtttc cgattcactg ggccaggggg ggatggacgt acttcgccgg gagaaaaatc
     3301 tcaaggtcga cgaaaaggtc ggtctgaaac ccgaggaact gaaggcgatt atcggggatt
     3361 atgatgctat tcttgttcgt agcgcgacca aggtgacgaa agaaatcatc gaggccggaa
     3421 aaaaattgcg cgtgatcggc cgggccggcg tcggcgtgga caacgtggat cttgaagccg
     3481 cgacccatcg cggcattatc gtcatgaaca ctcccgaagg caataccatc tcgacggcgg
     3541 aactgagttt ttccatgctg atggcggtcg cgcggaatct cccgcaagcc gacgcgcacg
     3601 tgaaaaaggg agaatggaaa cggaacaagt tcaagggcag tgaattgaac ggaaagaccc
     3661 tcgggatcgt cggatttggc cgaatcggcc gagaggtcgc gaagcgcgcg aacgccttca
     3721 acatgaaaat tatggtgttc gatcccttta tttccaaaga atccgtcaag gaatggccag
     3781 ttgagttcgt ggatgtcccg acccttctcg ggaactcgga ttttcttaca tttcacacgc
     3841 cgctgacggc cgaaacccgg aaccttctca atagggagac gttcaaactt tgcaaaaagg
     3901 ggatgaggat cgtcaattgc gcgcgcggag ggattgttga tgaaaccgcg cttgccgagg
     3961 ctttgcggag cgggcagatc gccggggccg cgtttgacgt cttttcgagc gaaccgcctc
     4021 ccgcggacca tccgctcctg aaacttcccc agtttatctg caccccgcat ttgggcgccg
     4081 tcaccaatga agcgcaggaa aatgtggcgc tggatgtcgc cgtccaggtg attgacgccc
     4141 tgaatgaccg ggcgatcaaa aacgcggtga atatgcccaa tctggatccc gatacgctca
     4201 aggggctgaa accgtggctt gtcctcgcgg aaaagatcgg gtcgctttat ccccagcttt
     4261 ttgacggcag tatcaaaaaa gtagaggtcc gctacggagg cgatcccacc cgttttaaaa
     4321 cggccccgct gaccgtggcc attctgaaag ggattttgac cccgatttgt ggcgataccg
     4381 tgaattttgt cagcgccccg aacatcgcca aagaacgggg aattaacgtt tccgaaggga
     4441 cgagcgacgc gacgatcgac ttctcgagct ttatcgatgt cgaggtgctt cagggcaagg
     4501 aaaaataccg gattatggga accctattta acaaagaacc gcgcatcgtc aagatcaatg
     4561 acgtcctgat cgacgtggtg ccggaagggt gggtcctttt tatccgaaac gaggatttgc
     4621 cgggcgtggt gggaagcatc ggaacgattc tcgggaaaaa cggggtcaac attgccgaga
     4681 tgtcgctcgg acgcgtggga aaagggaaaa aggctttcgc gatgacggcg atcaatattg
     4741 acagcgaagt ccccgctaaa gttgtggcgc aaatcaagaa ggcgaagccg gtcattgacg
     4801 taagggttgt gaaactataa taaagtaccc ggggacagtc cctccgcgaa tccggagact
     4861 gtccccgttt tctgtccact gttaaaaggg agaatctcaa attgaattgt attttaatcg
     4921 gcgctcagtg gggcgacgaa ggtaagggta aagtcattga cattcttgcc gcgaaaagcg
     4981 acgtggtggt tcgttaccag ggcggcccga atgccggcca caccatcaag gtgaaaggtc
     5041 ggaaattcgt attgcatctt attccttccg gcgtgcttta ccccaaaaag gtttgcgtca
     5101 tcgggaacgg agtcgtgatt gatccggaat cgctttttga agaaattgaa atgctggatc
     5161 gcaacggtat caggatccgt aaccggtttt ttatttccga tcaggcccat ctgatttttc
     5221 cttatcatcg gctgatggat cagttgaggg aggcgagcct taaaaaaggg aaagccatcg
     5281 gcacgaccaa acgggggatc gggccctgtt acgcggataa aatggcgcgc gtcgggatcc
     5341 ggatctgtga cttgcggtca atggattatc tccggcaaag gctttcggac gtggtgaagg
     5401 atcgtaacgc ggttttgacc aaggtttacg gacatcgtcc catttctttc gcgaagactt
     5461 tcgcctattg tcaaaaaatc cggggacggt tgctggaact tgccatcgat acccccaggt
     5521 atctttacga cgcggtccgg gcccgtaaga aaatcctttt tgaaggggcc caggggaccc
     5581 tgcttgacgt ggatcacggg acttatcctt ttgtcacttc gtcaaacgcc acggtgggag
     5641 gcgcgatcac gggatccgga attgccccga cgttcattga ccggatcatc ggcgtggtga
     5701 aggcctatac gacgcgcgtg ggagaagggc cgtttcccac gcagtttcct cccgatttga
     5761 tggaaattgt tcaaaaaaaa ggagaagaat tcggatccac gaccggccgc gcgagacggt
     5821 gtgggtggtt tgacgccgtg atcgcggaac acgcggtgcg gattaacggg ctggatgaaa
     5881 tggcgatcat gaaactggac gtcctttcgg gcatgaaaga gctcaagatc gcgattgcct
     5941 acaaggtgaa cggaaaaatg actcgtgatt atccgcacgc cgtcaacgaa ttttcttcgg
     6001 cgaaacccgt ctatgagacg cttccgggat gggatgaaga cattacggga tgtaaaaagt
     6061 ggaacgatct tcccttgaac gcgcgtcgat acctgaaaag aattgaacag ctgaccggca
     6121 ctcccatcaa gattgtttcg gtcggcagtc agcgacatca gaccattttt ctctaaaaaa
     6181 ggcgggccct atgctgaatg aaattcccaa gcacgtcgcc gtgatcatgg atgggaacgg
     6241 ccgttgggcc aaaagtaaag gttttccccg aatcaagggc cacgaggaag gcgtgcggcg
     6301 cgtcgaagaa atcgttaagg tcgcggcgca aaagggtatt cgctacctga ccctctacac
     6361 cttctccaag gaaaattgga ggcggcccaa ggaagaagtc aattttttga tgggccttct
     6421 ttcgaggtat ctggatgaga agattaacac gctcattgag gacaatgtcg ttttgaacgt
     6481 gatcggggag atgagcgatc ttcccgagac tatccaaaag aaacttcggg atgcccggaa
     6541 aaattccgaa cgaaacacgg ggctgaccct gacgctggcc ctcagttatt cctcgcgcgc
     6601 cgaaatcttg cgagccatca aaaaaattgc ggatcgcgtg aagaaacagg acctggacat
     6661 cgcagggatc gacgaatcgc tcgtcaacgc gaatcttgac acggccggaa ttcccgaccc
     6721 ggagcttttg atccgcacga gcggtgagat gaggatcagt aactttttgc tctggcagat
     6781 ttcgtacgcc gaactttacg tgacgccgaa gttttggcct gagttcactt ccgaagaatt
     6841 tgaaaaagcg atcgatgaat accgtcgccg ggaacgccgt tttggcgcca ccgaacccgc
     6901 gcgaggactt ccgtcgtgag agcgcctctg gattgggcca aggccctgaa gatggtatgt
     6961 ccgctcggaa aaaaaacaga tcagttgggg aacctatgtt aaataaagtg acgataggca
     7021 cgcttgggaa aaggctcctc atgtctttaa ttatggtgcc ggcggtcgtg gcgacgattt
     7081 tttacgctcc tcactggctc ttttttctga tggtcgtggg atttatcatg gtggccctca
     7141 atgagttttt ttcgatcgcg gaaaacaagg ggctaattat tcaccggggc tttggtcttt
     7201 gctttggagc tttgctcccg ttcgcggctt acgcgtccct gcaacttccg ctggtgttgg
     7261 ccgcctgcgt cgcgcttttt attttcctgt ttaaccgccg gtccccggat cgaaccatca
     7321 ccagtgtcgc ggtcagtgtt ttcgggattg tttacgtggc ctggtttttt tcttacctca
     7381 ttaaactgcg attgcttccg aacggcgccg aatgggtttt ttacacgatc ctgatcgtca
     7441 aggggggaga cgccggcgct tatttcatcg gccgaaaatt cggaaaaaac aaactgcttc
     7501 cgctggtcag tcccaacaaa tccgtggaag gggcgatcgg acaaatggtg acgacgcttg
     7561 cgctttccgt tggggcggtt ttttttatcg atgtgtcctg gtaccacttc gtgtttctcg
     7621 gaattggggt gggcgggctt gccgttttcg gggatttggc cgagagcttg atcaagcggg
     7681 acgccggtgt taaagattcg ggacagatgc cgggtcttgg cggcatgctg gacgtggtcg
     7741 acagccttct ttttacggtc cctttcgtgt atttttacct cacttttgtt ctcggccttg
     7801 aacggatatg agcggggtca agcgtctcac catccttggt tccaccgggt cggtgggaga
     7861 gaacactctc aggattgtca ggatccatcc tgaccggttt cgggtcgagg ccctggccgc
     7921 caaaaaaagt attgcccgct tgtacgaaca ggcggttgaa tttcgcccca aagtggtttg
     7981 cgtctacgat gagtcgctcc gggacaagac atgggtcgcg aaatttaaaa aacttggcgt
     8041 gaagctcgtg agcggggatt cgggccttga ggaggtttcc acgattcctt cggtccggga
     8101 ggtcatctgc gccatggttg gcgcggttgg tcttcagccg attttttccg cggttcgcgc
     8161 gggtaaaatc atcgcggtcg ccaataaaga gccgctggtg atggctggaa gccttttgct
     8221 taaagaggcc gcgaaacggg gagtcgcggt tttgccgatc gacagtgagc attcggcgtt
     8281 gtggcagtgc ctcgaaaatc agcatcctga tttcgtcaaa aaactgatcc tgacttcttc
     8341 gggtggcccg ttccggaatt tccggggctc tctcgcgaac gttacgcggg cccaggcgct
     8401 taatcacccg aaatggaaaa tgggccccaa aatcacgatc gattcagcga cgctcatgaa
     8461 caaggggctc gaggtgattg aggccgccaa cctttttcgg gtagaagcaa aaaagattga
     8521 agttttaatc catcccgaag cgatcgttca tgccttggtg gagttggccg atggctcaca
     8581 tctggcgcat ctcggggtgg ccgacatgcg tcttcccatt cagtatgcca tgagttatcc
     8641 ggagcggctc gacaatcatc tcccgaacct tgatctcacc cggctccggg gacttcactt
     8701 tgaaaaaccg gatacccgtc gtttcccgtg tctcaaactt ggttatgagg caagcaagat
     8761 cggagggacg atgccggcag tcctgaacgc ggccaatgag attgccgtgc aggccttttt
     8821 ggatcaaaag gccggattct tggacattcc gaagacgatc gaaaaaacaa tgacgcggca
     8881 tcggctggtg cgcgaacccg gccttgagga catccttgaa tcagatctct gggcccgtca
     8941 gatcgcccgg gaatttattt agttgagggg ccggagagtt tccccggccc gttcactctt
     9001 aattgaacgc gagtcgaatg aggtgaaata atcatgcaaa atcttttatc ggtttttgtg
     9061 aacgtcctcc cggcgattgt ggtgtttggc attttgatta tagtccatga atgggggcac
     9121 ttcatcgcgt gccggttacg tggtgtcaag gtcgagaagt tttctatcgg atttggtccc
     9181 gagcttttcg gctggcgttc ccgggaaacg cattttgttg tttcggcgtt tccgctcggg
     9241 gggtttgtga agcccgcggg ggaagataca ctatcggtgg gggccgaggg ggccaaaccc
     9301 ggtgattttc tggccgcgtc ggtcgggtcg cgaatgatga tcgtgatagc tggcgtaatc
     9361 atgaattttt ttctggcgtt cgtgatcttt tccgtggtgt acatgatcgg acgcccgctc
     9421 cccggaacgg taatcggcgg ttttatccag ggatatcccg ccgaggcgag cggacttgaa
     9481 accaaagata aaatcctgaa ggtggacggg acgcccgttt ttacctggaa ggacgtgctc
     9541 gaaacgctgg accgcagtcg cggcgaatcg ctatcaattg aagtgtcgcg ggggggaaag
     9601 gtagtttcct tttccctgag tccgaaaatt gaagaaacca gggatatttt tgggaaaaag
     9661 gtgacgttga aacgagtggg gattacgccg gatcccgagg tggttgtgtc ggagcggtat
     9721 ggattttttc aatcaatcgc ccggggagga gaggcggtcc tttttcacac gaccttgacc
     9781 tataaggcga tttactatat ttgcgcccgt cagctttccc cgaaaaatct gatgggaccc
     9841 cttggcatta tccaggtcag cggtcaggcc gcgaaggcgg gagtagcgtc acttcttttg
     9901 ctcacggcga tcttgagcgt ttcgctcgcg gtgatcaact tgttcccgat ccccgcgctg
     9961 gatggagggc atctggtttt tttgctgatt gaggcgctgt tccgtaaacc ggttcccgcg
    10021 cccatccagg aaaaagtcac gaacgctggc tttttcgcgc ttatggggtt gatggttctt
    10081 gtgttttata acgatattgt caacttgcag attctggaca aactaaaaaa tctactgcat
    10141 attagcggca gttaaaacag cgcggcgccg cggcccgcgt aaaggatttt atcatgcgtt
    10201 ggacacaaac actgattccc agtcttagaa acgttcccaa agacgccgag gcgctcagcc
    10261 atcagctggc gctcagggcc gggctgatcc gccagctcgc atcgggtgtt tacagctatt
    10321 tgcctctcgg gttcagagtt cttcacaagg tgatcgggat tattcgcgaa gaaatgaaca
    10381 aggcgggcgc gtccgaagtc ctgctacccg cccttcatcc ggccgagatt tggaaaaagt
    10441 cgggccgcta cgaaacgctg ggggaagaca agatcgcgtt taagaaccgg gcgggccacg
    10501 agtttgtgtt gggccccacc catgaagaag tgatcaccga tctggtggga acgaacataa
    10561 aatccttccg ggacctgcct ttgacccttt atcagatcca gactaaattt cgtgatgagg
    10621 cccgtccgcg atttggagtg atccggacga aggagtttat catgaaagac gcttacagct
    10681 ttgaccgcga cgaggcggga ctggattcac attatcaaaa aatgcgcgcg gcttaccaca
    10741 atatttttaa tcgttgtgga cttaagtttc aggtggtgag cgcggatacc ggaatgatgg
    10801 ggggcaaaat gagccaggaa tttatggtca tttgccctta cggggaagac cgtgtcgcga
    10861 gttctgaaag cggttatctc gcgagccagg acatcgcgga gcgagccagg cccgaacttt
    10921 ccgtttccca gcctcccctg gaaaaaccgg aaccctttga cactcccaac ctcaggacga
    10981 tcgacgagat ttctcaccga tttaaaatag ctccgtcgcg gttaatcaaa acgttaattt
    11041 ttatcgcgga cgaccgccct gtcgccgctc tcgtgacggg ggacagtgag gtcaacgaag
    11101 gaaaactccg gaagctgatt ggggcaaaaa cgattcgcca ggctgaggcc cacgagattc
    11161 gcgaagcgac gggcgctccg gtcgggttcg cgggccccgt cggacttaaa attccgatgt
    11221 acgcggattg ggacgtgacg gccgtcacgg acggcgtcac gggcgccaat gaaaaggaca
    11281 agcatctccg gcatgtggct tacgggcgtg attttacgcc cgtggcggcc ggtgacatcc
    11341 gtttcgttaa agacggggat ctcgcgcctg atgggaaagg ccggttgacg ttgacgacca
    11401 gtatggagat aggtcacatt ttcaagctgg ggacacgata ttccgaatcg atcggcgcga
    11461 cttttcttga cgaaaaaggg gagaggaaac ccgcgatcat gggctgttat ggaatcggcg
    11521 tgaaccggat tctcgcggcg acaattgaac agcatcatga tgaaaaagga atcaagtggc
    11581 cctccgagat cgcgcctttt caggttgaaa tcattacgat taatcaggcg gatcccaaaa
    11641 tcacgaccgc ggccgatcga ctttacgatg agttgaacgc ggccggcgtg gaggttctct
    11701 atgatgaccg cgacgaacgc gcaggcgtca agtttaacga cgcggacctg atcggtatcc
    11761 ctaggcaggt cgtgatcgga gaacgcaatc tcgctcaggg gaaggttgag atcaaggatc
    11821 ggcagacggg tttgacgaaa atgatggatc tgtgttcagt ggtttcttct cttttataga
    11881 tttgagcgtg gcgtatcgga tccgcgtttg tctcgcgcgc ctacaccttg cgccgcgcgc
    11941 gtgtgcttaa atgaattggc cccgtaaatt cattcatatc gatatggacg cgtttttcgc
    12001 ggccgtggaa caacgtgacc ggcccgagct caggggaaag cccgtcattg tcggagggga
    12061 tcctcaaagc cggagcgtgg tcagcacctg ctcttatgaa gcccggcggt ttggaatcca
    12121 ttccgccatg ccgtcctcac gagccaggcg cctttgtccc caggctatct tcatccttcc
    12181 ggactttgaa aaatattccg cagtctcgaa aacggtcatg tcgatcctcc gcaattacac
    12241 gccgctcgtg gagcaggtat cactggatga ggcttatctt gatgtgatgg aaaacaaact
    12301 ccggatcgac gatcccgtcg ggctcgcgaa actgatcaaa cagcatatct tcgcggcaac
    12361 ccgtctcacg gcttccgcgg ggattgccac ggccatgttc ctcgcgaaaa tagcttctga
    12421 tttcagtaag ccggacggct taacggtgat tcctccccgc gaggaagtcg tttttctaaa
    12481 aaatcttccc gttcgaaaac ttcccggagt gggacccaag accgaggcgg tgctccaccg
    12541 gatcggggtg aggacctgcg gggatcttcg ggagatgtct ccccagagcc ttcgaggcca
    12601 tttcggaaaa tggggaaacc acctttctca aatggccaac gggacagagg agcgggaggt
    12661 ggtacctttt tgggagccga cccaggtgag taaggaagag acatttgaaa aggacatcct
    12721 tcgggtggat tggctcaagg caaaactggg gaaactttca cggcaagtgt tcaccgaact
    12781 ggtttataaa gggaaatcgg gacggacgat cgtcctgaaa gtcaaatacc acgatttcga
    12841 acaaatgacc cggtcccgga ccttg
//
LOCUS       DAOSIO010000021        12673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_5006_length_12673_cov_4.266683, whole genome shotgun sequence.
ACCESSION   DAOSIO010000021 DAOSIO010000000
VERSION     DAOSIO010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 12673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12673
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5006_length_12673_cov_4.266683"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3407
                     /locus_tag="PK590_01640"
     CDS             <1..3407
                     /locus_tag="PK590_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23331.1"
                     /translation="DPVKVEVLREYIRLTQDETIAGYRFDTIELATVTGKPAGKSPEG
                     DSMIFPADPANPYLGGSQGREKATRQGGFFALERVLHHQRKSSVKGVSYALQGFGNVA
                     GFFARIANRSGMVAKAIQSAYPAGKDETGKTRFVQFTIYNEKGIDIAALDQWILSESN
                     RLGKKPGEFSLENFPGARVIANEDFWKLNVDVMVPAATNNVITEKNAGLINAPVILEL
                     ANGPTSVEGDKILLSRGKLVIPDVLANAGGVIVSYFEMIQNYTHDRWTTAMVDRMLSE
                     RMSQATDDAMRIMNSYGKTFRQAVYMSALMKIADAQIARDPELKARYEDGTIQPYDSH
                     GLSRRPETTNHLNFLTQRDRLAALLAETAREFNAEIVARVDRISGRFSKGVARNPVVL
                     ITGPESAGKEVVAESIVKELQRREDFKQSGRKVHFVDYDLVNPEEFEQLLSGQRTQLL
                     QPQYTFYGMPVWTPITVNVGDVIVVEGRAAFSVANLKPKDQGANLYFGKEDVYKIFVN
                     VPPSFNLTVSKRDQAFVPKGMPLTSLHLRFIREILDRQNRRGETMTGAGIVGLLEGWH
                     GLKEMQNRTVYPQWSATDETINMYFSEEIAVIRNQALRLLVLALRHARQELPGSLMLA
                     RQMESAIAFLREVNPVNPEELPEGHPVRQWAVNHQATTRDGFERNREGGRDGSARLSR
                     AEARVSLDTPTLIGMVIKIVTDYRSDQPFPLEARSLLWSAKTAIGNGDFEGAIKSLDK
                     ANVALAGLASDYTRRRIIQLRDVLSKRIARSENRSENGEDETIEEGSRWGKVFEATFY
                     AGSFVIAANVPLLLYAYAVGRWANQMEGFTAVPFFLTIGGTTVILMGIGAGIFNGLLF
                     VGKRFFGFSPLKTVLQDKGVSRRAPSEPKPESDDSVSRKRSEARSTSVLMEEMLFPTQ
                     LIEERWDLKQVINPFMLTLIREFYYGIIPERVREGIETGLNTAGQALRAMMEAVGVSP
                     ADATGLSTADRKEIFQRALEILGIRKPSVDAADVFVLGAELLKKLRENDGRGLVAFRE
                     ALQGSLVAVVTDSETLASINQILGEAGLEPYLSTQKLKNHLPKGAVVRAVLLAGETLS
                     EEDNRLLRIAERNVIRMTQGMLDRFMLVMEQIVTAIMGEKAISRSA"
     gene            3639..4853
                     /gene="tyrS"
                     /locus_tag="PK590_01645"
     CDS             3639..4853
                     /gene="tyrS"
                     /locus_tag="PK590_01645"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOG23332.1"
                     /translation="MFPSVKEQISVFKENLVELISERELEAKLTESLQKNRPLRIKYG
                     ADPSAPDLHLGHTVPLRKLRQLQDLGHQVVFIIGDFTAQIGDPSGRSETRKMLSPEDV
                     ARNAKTYQDQVFQILDKKKTEVRYNSEWLAKMTPGDFLRLTGQYTVARLIERDDFSKR
                     YKAGDPIALVEFLYPLMQGHDSVVVRSDVELGGTDQKFNLLVGRELQKTDGQAPQVVM
                     TLPIIEGTDGVQKMSKSLNNHIALKDSPREMFGKVMSISDPMMERFYRYVSAIPVKEV
                     EAIIQGLKTGQLHPREMKASLAERIVALFHGAENGKRAREEFDEIFKKKGLPDQIPTV
                     KFLKSEMDIVSILVEAGLVASKGEARRLIEQGGVKVDQVKVTDVKQVIKLDHELIVQC
                     GKRKFVKIESAS"
     gene            5050..6066
                     /locus_tag="PK590_01650"
     CDS             5050..6066
                     /locus_tag="PK590_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG23333.1"
                     /translation="MVTIRKPRSTEGLVRFESVTKRYGNKFAVENLSFEIAQGEVVGF
                     LGPNGAGKTTAMRILAGYFSPTSGRVILDGMDLFKEPQRAKRHVGYLPEVVRLYPDMR
                     VREYLRYVAELKGIRSQSIPMEIEEKISLCGLTDVERRLIGRLSKGFRQRVGLAQALI
                     GDPPVLVLDEPTTGLDPKQISEIRALIRDLGKRRAVILSTHILPEVSMICNRVIMIHQ
                     GKVLASGTVRELESCLKDREAIFVTVPGAQYQETAEGILSKISGVENVRVVERHENEI
                     QFCLETAQDEDLRPEITKFFVESKIPVLEIRRDQLTLEEIFLKLLKDGWFQQRTKIEA
                     KAET"
     gene            6146..6925
                     /locus_tag="PK590_01655"
     CDS             6146..6925
                     /locus_tag="PK590_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOG23334.1"
                     /translation="MRKVFAIARKEFGSFFHSWTGVMTAVMFFAVTGIFFVILTLNFA
                     QISMDPRSAGLSSFPNISQTHYVFGSLFLNLNVILLFLVPLITMRSFAEERKHETLEL
                     LFTYPLTDLEIVAGKLLGLIWFFEMLLVPLAGYALVFYWIGGELDWGPVLSAFLGFWV
                     LGMAYLSAGVFVSTLTKSSVMSAVGTFGILVIFWMMDFAPMSGGGGVSVVVNAMSPLR
                     HFRNFTYGILDLRDLVYFAFFFFYFLFLSLRSIETRNWKES"
     gene            7037..8440
                     /locus_tag="PK590_01660"
     CDS             7037..8440
                     /locus_tag="PK590_01660"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOG23335.1"
                     /translation="MRRFFLKFQLILEAALLFATLILVGVILDRYDYRWDLTQEKVYS
                     LPPVTSGVLKELSSQPVEVYAFYPQGDDARRGLEVFLRECQRYHPDLRYYFYDPARRP
                     GMAKKFQITQPATILIRAGEREERVVDPNEEAFTNAFLRILHPRNVDVCFTSGHGEVQ
                     LAGEDPTSYQMFRVALEGYNARIHEIVLLRDHVPDVCQVVVVAGPRWELVPGELADLK
                     SAFERGKGVLLLLDPMDPGTGQSFIEFAKSMGVSLSENVVVDKTSRIVGGDFLMPLVS
                     QYFVNHPVSKTMKQATFFPLLRSVQPSSDSIPGLEVVPLGMTSAGSWAETDLAALENG
                     YATFDVEKDIAGPLSIAVAIEQKTDDEKPDASGVQPTGSSLSPAGRMIMIGDSDFLNN
                     SYLNLSGNKEFGLRIMQWLAKDERFVEVKKPQFKFKPLMLDVPKQTFLILSSLVMYPL
                     IFFIGGGLYLVIRSRRS"
     gene            8437..9420
                     /locus_tag="PK590_01665"
     CDS             8437..9420
                     /locus_tag="PK590_01665"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOG23336.1"
                     /translation="MKLFRTLVIGVIFALAVSVYVYQSRLDQEILSLNPDEVNRSFTL
                     GPDQHVDRIAIHDAVKNTEIVLERAEEGWRVESPVSYPADSRTVEGLVMAARFAASQT
                     RLRAEKDWTEYGLDKPGLELRVETRAKREGVLIFGDASPVGKALYARWTGERGYILVP
                     PEIKSVFSVSVYGLREKAIFQTPPAEMVKVYVEMGPNAYEWKKEGGRWFWMEPIEKFG
                     REIAEDRMQLIFKGLAGLNVMEFLDDNQKSDAELGFFIIHDKIKITHHSGEEEIFHFG
                     NEVPLKNSYYGKMEKNGVVVLVDRMKIIQFLDLMKALEVSADRTVSSHAEA"
     gene            9501..10190
                     /locus_tag="PK590_01670"
     CDS             9501..10190
                     /locus_tag="PK590_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOG23337.1"
                     /translation="MTLVERIQNLKQSINRWATRDSHDPALVRLVIVTKTVPPEMIRS
                     AYDAGERDFGENRVQEWREKKEELPTDIRWHLIGHLQTNKVKYVVGQVHLIHSLDRLE
                     LADEIERQAQKKGIHEVPCLLQVNMSGEETKSGMDPDQVSRFAAAMANRPSIKVKGLM
                     TIGPFTEDEARIRECFRATRRLFESLKTEQRSHAWEILSMGMSADYKIAIEEGANMLR
                     IGSLIFPRNLK"
     gene            10201..10992
                     /gene="proC"
                     /locus_tag="PK590_01675"
     CDS             10201..10992
                     /gene="proC"
                     /locus_tag="PK590_01675"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOG23338.1"
                     /translation="MKQKIGFIGAGNMGSAILEGVLKKKIVSASRIIVYDKIAAKSQM
                     LSKRFHVARADCIGDVFRESDVVLLAIKPQDFKEFAAETRDLIRQGQWIVSILAGLSV
                     DRIREEMGRVDVVRAMPNLGAKVGQSMTVITGSKKRLALAEKIFQGCGDVVRLPEKCF
                     DDVTAVSGSGPAYFFYLMELMSEFALRKGFPADVATRLAIQTGLGAVLVAKNSEVSCS
                     QLRKMVTSRKGTTEAALTVLQRRKFGRIFQSALVAASKRSRQLRK"
     gene            11073..11378
                     /locus_tag="PK590_01680"
     CDS             11073..11378
                     /locus_tag="PK590_01680"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOG23339.1"
                     /translation="MFIIAEILKALAMLVNGITTILYWLLFARIIVSWIPVDPYSSIV
                     QFLYQVTDPILSWFRQIPLRIGAIDFSPILAFIFLYFVNRVLVTILLTIAYKFQGGM"
     gene            11378..12226
                     /locus_tag="PK590_01685"
     CDS             11378..12226
                     /locus_tag="PK590_01685"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOG23340.1"
                     /translation="MAMKRFCREKNGLMDRIGECLAYMREKTDFKPEIALILGTGLGR
                     LADRIKPERILAYDELPHFPQSTVQSHKGQLVLGEIAGKKVAAMEGRFHYYEGYSLEE
                     VTFAVRVLRELGTRKLVVSNAAGGLNLEYRKGEIVLITDHINFMGVNPLIGPNEEKLG
                     PRFPDMCEPYSRRLIDLALKSAQEVQIPVRQGVYIGVAGPCLETRAEYRMMRHWGADL
                     VGMSTVPEVIVAVHMGMEVLGVSVVTDLCDPDHLEPCDIQEIIKTANEAGPKLDKLIA
                     ATIQKF"
     gene            12332..>12673
                     /locus_tag="PK590_01690"
     CDS             12332..>12673
                     /locus_tag="PK590_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG23341.1"
                     /translation="MPIEKSYKNTLNLPQTSFPMKANLVQREPEILNRWETEGIYKKV
                     REKSEGKPNYTLHDGPPYANGRIHIGHALNKVLKDLIVKFQTMRGYDAFYVPWWDCHG
                     LPIEHACLKEMG"
BASE COUNT         3095 a         2738 c         3588 g         3252 t
ORIGIN      
        1 cggatcctgt taaggtggag gtgcttcgtg agtacatccg tctgacccag gatgaaacga
       61 tagccggtta tcgatttgat acgatcgaac ttgcaacagt gaccggaaaa cctgccggga
      121 aatctcccga aggagattcg atgatattcc ccgcggatcc cgccaatccc tatcttggag
      181 gttcccaggg gcgggaaaag gccacaagac aaggtggatt ttttgccctg gagcgggttc
      241 ttcaccacca gaggaagtcc tcggtcaaag gggtgagtta tgcccttcag gggtttggaa
      301 atgtggctgg tttttttgcc cgtattgcaa atcggtcagg catggttgcg aaggccattc
      361 agtcggctta cccggccgga aaggatgaga caggcaagac ccgtttcgtc caatttacaa
      421 tttataacga aaaaggaatt gacatcgcgg cccttgatca atggatttta agcgagtcca
      481 accggcttgg gaaaaaaccg ggcgagtttt cgctggagaa tttccccggg gcccgtgtga
      541 ttgcgaatga ggatttctgg aagctgaacg tggacgtgat ggtgcccgcc gcgaccaaca
      601 atgtcattac cgagaagaat gccggattga ttaatgcccc cgtgattctt gaactcgcga
      661 atggacctac ctcggtggaa ggggataaga tattgctttc tcgcggcaaa ctggtgatcc
      721 ccgatgtgct tgcgaacgcg gggggcgtga ttgtgtccta ttttgagatg atacaaaatt
      781 atacgcatga tcgctggaca acggcgatgg tggaccggat gctttctgag cggatgagcc
      841 aggccacgga tgacgcgatg agaattatga attcttacgg gaaaaccttt cgtcaggcgg
      901 tttacatgtc ggcgcttatg aagattgctg acgcccagat tgccagggat cctgaattga
      961 aagcccggta tgaggacggg actatacaac cctacgattc tcacggactg tcacgccgcc
     1021 cggagacaac gaatcatttg aattttctga cgcaaagaga ccgacttgcg gcattgcttg
     1081 cggaaaccgc gcgagaattt aatgcggaaa tcgtggcaag agttgacaga atatcggggc
     1141 gattttcgaa aggggttgcc cggaatccgg tcgtactgat taccggtcct gaatctgccg
     1201 gtaaggaggt ggttgctgaa tcgattgtga aggagttgca gagaagagag gattttaaac
     1261 agtcgggccg caaggtgcat tttgttgatt atgaccttgt aaatcctgag gaattcgaac
     1321 agctattgag tggtcagcgt actcagttat tacagccaca gtacacgttt tatgggatgc
     1381 cggtatggac tcccatcacg gtgaatgtgg gtgacgtgat tgtggtagaa gggcgtgccg
     1441 cgttttctgt ggccaatctt aagccgaaag accaaggcgc gaatctgtat tttggtaaag
     1501 aagatgttta caagatcttc gttaacgttc caccgagttt taatttaacg gtttccaaac
     1561 gggatcaggc atttgtcccg aaaggaatgc cgctgacgtc attgcatctc cggtttattc
     1621 gtgagattct ggaccgccag aatcgacgtg gcgaaacaat gactggtgcc ggtatcgtgg
     1681 gcctactcga aggatggcat gggttaaagg aaatgcaaaa ccggacggtc tatccccaat
     1741 ggtccgctac cgatgaaacc attaacatgt atttttcgga ggaaatagcc gtcattcgaa
     1801 accaggcgct cagacttttg gttcttgcgt tgaggcatgc tcgacaggag ttgccgggtt
     1861 cactgatgct tgcccgacag atggaaagcg cgatcgcttt tttgcgtgaa gttaatccgg
     1921 ttaatcccga agagcttccg gaaggccatc cggttcgcca gtgggcggtt aaccatcaag
     1981 caacaaccag ggatggtttt gagagaaatc gagagggcgg ccgagatgga tcggctcgtt
     2041 tgtctcgcgc ggaagctcgc gtcagcctgg ataccccaac tctgattggc atggttataa
     2101 agattgtaac ggattacagg agtgaccagc cattcccgtt ggaagcgcga agtttgctct
     2161 ggagtgcgaa gactgctatt gggaatggtg atttcgaggg cgcgatcaag tcattagaca
     2221 aagctaacgt ggccttggct ggtttggcga gtgattatac gcgccggagg attattcagt
     2281 tgcgtgatgt tctttccaag cggattgctc gttcggagaa tcgttccgaa aatggtgaag
     2341 atgaaacgat tgaagagggg agccgttggg ggaaggtttt tgaggcaacc ttttacgcgg
     2401 gaagttttgt aatagcggcg aacgtaccac tcctccttta tgcgtacgcg gttgggcgtt
     2461 gggctaacca gatggaaggt tttacggctg tcccattctt tctcaccatt gggggaacca
     2521 ccgttattct gatggggatt ggggctggga tctttaatgg attgcttttt gtgggcaaaa
     2581 gattttttgg tttttctcca ttaaaaacgg tattgcagga taagggcgtg tctcgcaggg
     2641 ctccttctga gccaaaaccg gaatccgatg attcagtttc cagaaagagg agcgaagccc
     2701 ggtcgacttc cgttttgatg gaagaaatgt tatttccaac acagctgata gaggagcgtt
     2761 gggacctgaa acaggtgatc aatcctttca tgctgacttt gattcgggaa ttttattatg
     2821 gcatcattcc cgaacgtgtg cgtgagggga ttgaaacggg ccttaatact gcgggacaag
     2881 cgttgcgagc catgatggag gccgtgggtg tttcaccggc tgacgcgacc ggattgtcga
     2941 ccgctgacag aaaggaaata ttccagagag ctctggagat tttggggatc cggaaaccct
     3001 cggttgatgc agccgatgtg tttgttcttg gagcagagct tctgaagaag ttgcgggaaa
     3061 atgacggccg gggtcttgtg gcgtttcgtg aggcgttaca gggttccctg gttgccgttg
     3121 ttactgattc ggaaacgctg gcctcaatca atcagattct tggagaggcc ggactggagc
     3181 catacctttc cactcaaaaa ctcaaaaacc atcttcccaa aggcgctgtg gtcagggcgg
     3241 tgcttcttgc cggagaaact ctttctgaag aggataaccg tttactccgg attgcggagc
     3301 gaaacgttat cagaatgacg caaggcatgc tggatcgctt tatgctcgtc atggaacaga
     3361 ttgtgacagc gataatgggc gagaaggcga tcagccgaag cgcctaaaaa atagcgcaga
     3421 atcggggaca ctcattttaa atctgaaaag tgagtgtccc cgatgtttcc cgagagtatg
     3481 gttgatcggg aatttccagt tttttcttct ctcttttaat tttccccgtt agaatacagc
     3541 cgtttttgta actccttttg aaaccaatac ctggtttttg atgtccgccg gaaatgaagt
     3601 cagttgccgg aggctcaata cgatgtgtga gaggaaaaat gtttccatcc gttaaagaac
     3661 aaatttccgt ttttaaggaa aatcttgttg agcttatttc ggaacgtgaa cttgaagcga
     3721 aactgacgga atctcttcaa aagaaccgcc ctctccggat caaatacgga gccgatcctt
     3781 cggctcccga ccttcacctc ggacacacag ttcctttgcg taaacttcgc caacttcagg
     3841 atttaggcca tcaggtggtt tttattattg gcgattttac cgcccagatc ggagatcctt
     3901 cgggccgttc cgagacccga aaaatgttat ctccggagga tgtcgcgaga aacgcgaaga
     3961 cctaccagga tcaggttttt caaatcctcg acaagaagaa gaccgaagtc cgctacaatt
     4021 ctgagtggct ggcgaaaatg acacctgggg acttcctccg gctgacgggg caatacaccg
     4081 tggcgaggtt gattgagagg gatgattttt ccaagcgata caaggcgggt gatcccattg
     4141 ccctggttga gttcctttat cctctcatgc agggacatga ttctgtcgtg gtgcgttcgg
     4201 atgttgaact tggtggtacg gatcaaaagt ttaatctttt ggtgggccgg gaactccaga
     4261 aaacagatgg tcaagcgcca caggttgtga tgaccctgcc gatcatcgaa ggaacggacg
     4321 gagttcagaa gatgtcaaag agtctcaata atcacatcgc tctgaaggat tcgccgcgcg
     4381 agatgtttgg taaggtcatg tcgatctcgg atcccatgat ggagcggttt taccggtatg
     4441 taagcgccat tccggtaaaa gaagttgagg ccattattca gggccttaaa acagggcaac
     4501 tgcatccccg cgaaatgaaa gcgtctcttg ccgaacggat cgtggcgttg ttccatgggg
     4561 ctgaaaatgg caagcgcgcg cgggaggagt ttgacgagat tttcaaaaaa aaaggtctgc
     4621 cggatcaaat ccctacggta aagtttttaa agtccgagat ggacatcgtt tcgatacttg
     4681 tcgaggccgg gttagtggca agcaagggag aggcgcgcag gttgatcgaa caaggcggcg
     4741 tgaaggttga tcaggtgaaa gtgacggacg ttaagcaggt catcaagctt gaccacgaac
     4801 tcattgtgca atgtggaaaa cggaaattcg taaaaatcga atcggcttca taatatcaag
     4861 acctgttttc tgatttcctg aaaaataatg gggcgacaat atgcgtgaaa ccgatctccg
     4921 tagaaattcc gcggaagata ttgaaatgaa acgtaagtga acgggcgcgt gatgattgct
     4981 gctcttaaaa aatttacttg agacttgatg tgactacata ccacatatta cataccacat
     5041 actgaaatta tggttaccat ccgaaaaccc cgatcaaccg aaggcttggt ccgttttgaa
     5101 agcgttacca aacggtacgg caacaaattt gcagtcgaga atctttcctt tgaaattgcc
     5161 cagggagagg tggtcggttt tctcggaccc aacggggccg ggaagaccac cgccatgcga
     5221 attctcgcgg gctatttctc tccgacgtcg gggcgtgtca tcctggacgg catggatctt
     5281 tttaaagaac cccagcgggc aaaaagacat gtgggctatc ttcccgaagt cgtacgactt
     5341 tatccggata tgcgcgtcag ggagtacctc cggtacgtgg ccgaactcaa gggtatcagg
     5401 tcccaaagca taccgatgga aatagaagaa aaaatttccc tttgtggcct cacggacgtg
     5461 gaacgccgtt tgatcgggcg tctttccaag ggatttcgtc aacgggtcgg gcttgcccaa
     5521 gccctgatcg gggatcctcc ggttcttgtt ttggatgagc cgacgacagg attagatccg
     5581 aaacaaatct cggaaattcg cgcgctcatt cgggatctcg gcaagcggcg agcggtgatt
     5641 ctttcaacgc atattcttcc cgaagtgtcg atgatctgta acagggtcat catgattcat
     5701 caggggaaag tgcttgccag cgggacggtt cgggaacttg agtcttgcct caaggatcgt
     5761 gaagcgattt ttgtgacggt ccccggggcg caataccagg aaactgccga gggaattctt
     5821 tcaaagattt cgggtgttga aaatgtcagg gtggttgaac gccatgaaaa tgagatccag
     5881 ttttgcctgg aaacagccca ggacgaggat cttcgtcccg aaattaccaa gttctttgtg
     5941 gagagcaaaa ttccggtgct tgaaattcgc cgcgaccagc taacgctcga agaaattttt
     6001 cttaaattgc ttaaagatgg ctggttccaa caaagaacaa aaatcgaggc aaaagccgaa
     6061 acgtgaaaga ttcactaaat caaaatcgac actgcttaat gacctctgtt ttccgaaatt
     6121 ctgttgtgct catggagggc gagaaatgag aaaggttttt gcgattgcgc gaaaggagtt
     6181 cggctccttt tttcactcct ggacgggggt gatgacggcc gtcatgtttt ttgcggtgac
     6241 ggggattttt tttgtgatcc tgaccctgaa tttcgcgcag atttccatgg atccccgaag
     6301 cgcgggcctt tcgagtttcc ccaatattag ccagacgcat tatgttttcg ggtcgctttt
     6361 tttaaatctg aacgtcattt tgctgttttt ggttccgctt ataacgatgc gttcgttcgc
     6421 ggaagaaaga aaacacgaaa cccttgagct tttgtttacg taccccttga cggaccttga
     6481 aatcgtcgcc ggcaaattgc ttggtctgat ctggtttttt gagatgctcc tcgtcccttt
     6541 ggcgggttac gcgctggttt tttactggat cggcggagag ctggactggg ggcccgtttt
     6601 gtcggccttt ctgggtttct gggtactggg catggcgtat ctttcggcgg gtgtctttgt
     6661 ttcgacgctc acgaaaagtt cggtcatgag cgcggtggga actttcggaa tattggtgat
     6721 tttctggatg atggatttcg ctcccatgtc gggaggggga ggcgtgtctg tcgttgtgaa
     6781 cgcgatgtcg ccactgcggc atttcagaaa cttcacttac ggcattttag atcttcgtga
     6841 cctggtatat ttcgcgttct ttttctttta ttttcttttt ctgtcgcttc gttcgattga
     6901 aacgcggaac tggaaagagt cctgatggaa aaacatttgt ggggaaaatc ggcggatgat
     6961 tgttccccgg gaattggaaa ataaaaaatg aacttgtaaa accagttcga agccttcgat
     7021 acattttaaa aattttatga gacgattttt ccttaaattt cagctgatcc tggaggccgc
     7081 gcttcttttc gcgactttga ttctcgttgg cgtgattctc gaccggtatg attaccgttg
     7141 ggatctcacc caggaaaaag tgtattcgtt accgcccgtc acgtccggtg ttctcaagga
     7201 actgagttct caaccggtgg aggtgtatgc gttttatccc cagggagatg acgcgaggcg
     7261 cggcctcgag gttttcctca gagaatgtca gaggtatcat ccggatctgc ggtattattt
     7321 ttatgacccg gcacggcgtc ccgggatggc gaaaaaattt caaattaccc agcccgcgac
     7381 gatcctgatc cgcgcgggtg agcgggaaga aagggtcgtg gaccccaacg aagaggcctt
     7441 cacaaatgcc tttcttcgaa ttttgcatcc ccgaaacgtg gacgtttgtt ttacgagtgg
     7501 ccatggcgag gtccagctgg ccggggagga tcccacaagt tatcagatgt tccgggtcgc
     7561 tctggaaggc tacaacgccc ggattcacga gatcgtgctt ttacgtgatc atgttccgga
     7621 tgtttgtcag gtggtcgtgg tggcggggcc ccgttgggaa ttagtcccgg gagagctggc
     7681 agatctcaag agtgcttttg aacgcgggaa gggcgtcttg cttcttctcg atccgatgga
     7741 tcccgggacc ggtcaatcgt ttattgaatt tgcgaagtcg atgggagtta gcttgagtga
     7801 aaatgttgtg gtcgataaaa ccagccgtat tgtcggaggt gatttcctta tgcctctcgt
     7861 cagccaatac tttgtgaatc atccggtgtc gaaaaccatg aaacaggcga ccttttttcc
     7921 acttctcagg agcgttcaac cttcttcgga ctcgatcccg gggctggaag tggttccgct
     7981 ggggatgaca agcgccggaa gctgggctga aacagatttg gccgctcttg aaaatgggta
     8041 cgcgacgttt gatgttgaaa aggacatagc gggcccgctc tcgatagcgg tcgccattga
     8101 gcaaaaaaca gacgatgaaa aaccggacgc aagcggcgtt caaccgaccg gatcttctct
     8161 atcgcccgcc ggaagaatga ttatgattgg cgacagtgat tttctgaata acagttacct
     8221 caacctttcg ggcaataagg aatttgggtt gcggatcatg cagtggctcg ccaaggatga
     8281 acgttttgtc gaagttaaaa agccccagtt caagttcaag cccctgatgc tggacgtgcc
     8341 gaagcaaacc tttctcattc tatcttccct tgtcatgtac ccgctcattt tctttatcgg
     8401 gggcggactt taccttgtga tccggtcgcg ccgctcatga aacttttccg caccctggtt
     8461 atcggcgtta tttttgctct tgccgtgtca gtttatgttt accagagccg tctggatcag
     8521 gagattctct ccctgaaccc ggatgaagtg aaccggagtt ttacgctggg gccggatcag
     8581 catgtggatc gtatcgcgat tcacgatgcg gtgaaaaata cggaaattgt gctggaacgc
     8641 gcggaggaag gatggcgggt tgaatctccc gtgagttatc cggcggattc tcgaacggtc
     8701 gaaggtttgg tcatggcggc gcggtttgcc gcaagccaaa cccggcttcg ggctgaaaaa
     8761 gactggacag aatacgggct cgacaagccc gggctggagc ttcgtgttga aacccgcgcc
     8821 aagcgggaag gggttctgat tttcggggat gcttctcccg tcgggaaagc gctttacgca
     8881 cggtggaccg gggaacgcgg ttacatcctc gtcccgccgg agatcaagtc tgtttttagc
     8941 gtaagcgttt acggtttgcg tgagaaagca atctttcaga cgccgccggc ggaaatggtc
     9001 aaagtctatg ttgagatggg gccgaacgcc tatgagtgga aaaaggaagg cgggcgatgg
     9061 ttttggatgg agccgatcga aaaatttggc cgtgaaatcg cggaagatcg gatgcaactt
     9121 atttttaaag ggttggcggg gctcaatgtg atggagtttt tggatgataa tcaaaaatcc
     9181 gatgctgagc ttggtttttt tatcattcac gacaaaatta aaatcaccca tcattcaggg
     9241 gaagaggaga tttttcattt tggcaacgaa gtgccattga aaaattccta ttacgggaaa
     9301 atggaaaaaa atggcgtggt ggtcctggta gatcgcatga aaataatcca gtttctcgat
     9361 ctgatgaagg cgctcgaggt ttccgctgat cggactgttt cgagccacgc cgaggcatag
     9421 cttttttgag aaaatagcag gggaaatatc ttccgggata gcgcgatttt ccgaataagc
     9481 gcggagtgtt caaaaaactt atgacccttg ttgaaagaat tcaaaatctg aaacagtcga
     9541 tcaatcgttg ggcgacccgg gacagccacg atcccgccct ggttaggctc gtgatagtga
     9601 ccaaaaccgt tcctcccgaa atgattcgct cggcctacga tgccggtgag cgtgattttg
     9661 gggaaaatcg tgtccaggaa tggagggaga aaaaagaaga gctcccgact gacattcgtt
     9721 ggcatctgat tggacatttg caaacgaaca aggtcaaata tgtcgtgggc caagttcatc
     9781 taattcattc cttggatcgc ctggaactgg cggatgagat tgagcgacaa gcccaaaaaa
     9841 aagggatcca tgaagttcct tgcctgcttc aggtgaatat gtcgggagaa gagacgaagt
     9901 cgggcatgga tcctgaccag gtgtcccgtt ttgcggccgc catggctaac cggccgtcta
     9961 tcaaagtgaa ggggcttatg acgattggac cttttacgga ggatgaggcc aggattcgtg
    10021 agtgcttcag ggcaacacgt cggctctttg aatctcttaa aacggaacaa aggtcacacg
    10081 cctgggagat actctcaatg gggatgtcgg cggactataa gattgccatt gaagaaggtg
    10141 caaatatgct tagaattggt tctttaatct tccctcgaaa cttaaagtaa ggattttgtc
    10201 atgaagcaaa agatcggctt tatcggcgcg ggcaatatgg ggtccgcgat ccttgaaggg
    10261 gtgcttaaaa aaaagattgt aagtgcgtct cgtataatag tttatgataa aattgccgcg
    10321 aaatcacaaa tgctttccaa aaggtttcac gttgccaggg ccgattgtat tggtgatgtt
    10381 tttcgcgagt cagatgttgt tttgttggcg attaaacccc aggattttaa ggagtttgcg
    10441 gctgaaaccc gagatttaat ccggcaaggt caatggatcg tttcaatttt agccgggctt
    10501 tcggttgacc ggatccggga agaaatgggg cgggtagacg tggtgcgtgc catgcccaat
    10561 ctgggtgcca aggtggggca atcgatgacg gtgatcaccg ggagtaaaaa aagattggcc
    10621 cttgccgaaa aaatctttca gggatgcgga gatgtcgtcc gtcttccgga aaagtgtttt
    10681 gacgatgtta ccgcggtgag tggaagtgga ccggcgtact ttttttatct gatggagctt
    10741 atgtcggagt ttgccctgcg gaaaggattc cctgcggatg tcgcaacccg gcttgcgatc
    10801 cagacaggtt tgggagccgt gttggtcgcg aaaaattcag aggtttcgtg ttctcagctt
    10861 aggaaaatgg tcacctccag gaaagggacc acggaagccg cgttgacggt cctccaacgt
    10921 cgaaaattcg ggaggatttt tcagtctgcg ctggtggcgg cttcgaaacg aagccggcaa
    10981 ttaagaaagt aaaaaagaaa gacagggtgc agggttaggc gaaacaggga tcgattcctt
    11041 tttcgcttgg catcggttgt cggaaaggat ttatgtttat tatcgcggaa atattgaaag
    11101 cgctggcgat gttggtgaat ggcattacga ccattctgta ctggttgctc tttgcccgga
    11161 tcatcgtgtc ctggataccg gtagaccctt actcaagcat cgttcagttt ttatatcagg
    11221 tgacggatcc gattttgtcg tggttccggc aaattccttt gcggatcggg gctatagatt
    11281 tttccccgat tcttgctttt atttttctgt attttgtcaa tcgggtgctt gtgacgatac
    11341 ttttgacgat tgcgtataaa ttccaggggg gcatgtaatg gccatgaaac ggttttgcag
    11401 ggaaaagaac ggtttgatgg atcggatcgg agaatgtctt gcctacatga gggaaaaaac
    11461 agattttaaa ccggaaatcg ctttaattct gggaacaggg cttggccgat tggccgatcg
    11521 aatcaaaccg gagcggatat tggcttatga tgaattgccc cattttcctc agtcgaccgt
    11581 ccagtcgcac aaggggcagt tggtgttagg tgagattgcc ggaaaaaaag tcgcggcgat
    11641 ggagggacgg ttccactact atgaggggta ttcgctcgaa gaggttactt tcgccgtgcg
    11701 agtgctccgt gaacttggaa cgaggaagct ggtcgtttca aacgcggccg gcggattaaa
    11761 ccttgaatac cggaaaggag agattgtcct gattacggat cacatcaact ttatgggcgt
    11821 caatccgctg atcggaccca acgaggaaaa actgggaccg aggttccccg atatgtgcga
    11881 accttattcc cggcgcctga tagaccttgc gctgaaatcg gcgcaagagg ttcagatacc
    11941 ggtgcgacag ggagtttata tcggagtggc ggggccctgc cttgaaactc gcgcggaata
    12001 ccggatgatg cgccattggg gagcggatct tgtcggaatg tcgacggtac cggaggtgat
    12061 tgtggccgtt cacatgggga tggaggttct gggggtgagc gtggttacgg atctgtgtga
    12121 tccggatcac ctcgagccgt gcgatattca ggaaatcatc aagacggcga atgaagcggg
    12181 gcccaaactg gataaattga ttgccgcgac aattcaaaag ttctaaggtt tccgggtaac
    12241 tggaataata cacagagaac acagaatttt ttcatggagc gacacttgca agccgtcgcc
    12301 cggttttaag ataaaaagaa aatacggatt tatgccaatc gaaaagtcgt ataaaaacac
    12361 actcaatctc ccgcaaactt cttttcccat gaaggctaac ctcgtccaga gagagcctga
    12421 gattttgaac cgttgggaga cggaaggaat ttataaaaaa gtccgcgaaa aatccgaagg
    12481 gaagccaaac tatacgcttc atgacgggcc gccttacgcg aatggccgga ttcatattgg
    12541 acacgcgctg aacaaggtgc tgaaggattt gatcgtcaaa ttccagacca tgcgtggtta
    12601 tgatgcgttt tatgtcccct ggtgggattg tcacggcctg ccgattgagc acgcgtgcct
    12661 gaaggaaatg ggg
//
LOCUS       DAOSIO010000022        12506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_5078_length_12506_cov_4.718015, whole genome shotgun sequence.
ACCESSION   DAOSIO010000022 DAOSIO010000000
VERSION     DAOSIO010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 12506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12506
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5078_length_12506_cov_4.718015"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..6278
                     /locus_tag="PK590_01695"
     CDS             57..6278
                     /locus_tag="PK590_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOG23342.1"
                     /translation="MGFGTSVWHRLFKNDAYPNPSDSTSAGVRSADGASAVAAVHRHE
                     VAPLDPDSTVVAITNGDRREISSSVFRKIFKNLFPGEDPEFITAEQIVSVKRAAKEKL
                     EEEMQWGLDPDKIVISYSGRMVQEKAGRSRAFHTQNLRELVRLGAQVIIYGNVQSSIE
                     SREMFEELRRLSDELNGVGPGKLIVRTGWSIEDQRKLLAATDLQVQDSDRDRYRGTGA
                     AEYTEADVSVNGGLQMGPAWLEGIIQKQGIVLDRTRPGSGNTIIPASDNVTPDAANDD
                     PVAYMNAFRWVIDTFNNDRIHLASYQAASVRLSRVLNSDLPAAEYLRQFDRGARKKDA
                     LKELARRIAEAKKGPGFQAALDEALEKITRMDRNEAKEEFGANKLPAIMAVVTILKPE
                     LLDRIRLWNAREHEVLSALKNHPEMKDLFEKGMRRFHPIVMRDEELADEDRLAVSLSL
                     GGRNLVMVMDLAFNNYSQEEGKTWGRIGEIESIVSDPGAHYRVFDWSLDPGEKVTYPS
                     EHFGQKMMSDWMVGIPVRDRERDRNGLPQRGKSYQALSVSVVRSEARASQLDLPGLPL
                     EMEALAERSVTFIDRDMSVETVAERIQNNQVIYLAPPLEVGYDYGKYEEQLVQMAVTK
                     IGPALERVRRHEAGIRKPYERKVFILRGMVDPGTSKAVYDAIKWNAHPPKGDDYDFDL
                     VFQPDFTFLDARTEKTEEPIVVFGVMGEDDGKDAAFQAATKTVLDRIFGQFYQGKKIQ
                     YMRIRSAEMSKEMLIIYLGAKLAHFDDVAELSRAYGADLSAAAFGAGLDKRIRTIFTN
                     PSLGFGGRLFVYLHWIYAQRLYKAAAELEGQTHQKLNDRKMEIEQKIRSAVERLERGE
                     NITEIVKDLPPQLHLLFTIKTILKINKQNILDFLRSIERAYESMYGSGSLRGKKVALL
                     GVGYSSQSGQITASPALLLIERLIVDEGISEFVIADPDAKAAFMKWVEEKRGENPRFN
                     AVKFEFTDNIYDAAKVSDLTIIPTDSNKALRDIDIKRLGDALQGRPVFDGMNIFGLRA
                     DGTYRYKLEDVRDAGINLVGVGRLPLGPELDAQTGYRLGDSVVVRDVAGYRQVLQERG
                     GSVTAQQKSVTVIGGGYVGLVTAANLAGLGHRVRVVDIKAKQKEMDALNGPETAVPIY
                     EPGLREMIIDGKAKGLITFSTDLEPAVQAASIVYLAVGTPAQDTGEVDLKYILQASGD
                     IGEAIKKHGGAKTLVVKSTVTPDTFKKMDETLAAKGLKLGKDYALVSNPEFLREGQAI
                     QDVTQPDRTVLGFYAAMSLEDRKRAEKEILELWYPLMLKKEHTVLLTDTASSTIVKYL
                     ANSFLAISITLSNLFSSAAEADHADFMEVRMPFKADDRIGGNAFLNAGVGYGGSCFPK
                     DVLALNFSSKDATGHVLPLIDIATGFNEYFKTAVVARTVESVARFPQAARPLEGKSVI
                     MLGMAFKADTDDMREASSASVLHELLVRGATQVRLHDPILSLPNVPPREVILDHFLDT
                     LYKIFRDGEEFQKAYQQHKALWIARGEPVDGEEKNYFKRTYFPEKFIKTGRVVFEPDL
                     DKLGKADIVFLVTEWRQYKGLDLSRFKQTDRKLWVVDGRNLFYDRRDEISQFAEYRGI
                     GTAFFGPRSEAREAFAEVGRMITTKKLRLPKWVGDYSKATRNRVFATPEERMSVAVEL
                     SRLNVENGTGGPFGAVVFDRKSGKIFSMGVNLVVSERASYAHAEMVALTLAQERLKNF
                     SLDHDGREFELVTSSQPCAMCFGAIPWSGVKGVVVGARGEDVETITGFDEGPIHPEWD
                     KELLRRGIDVKRDILRDKAIEVLHAYVRKKGTIYNGKAESEKSVRSETRVYSGLEFQQ
                     IPEMNYEEFLAWYETQSKASRTAMENLTVEIMQGIAPLLANRFKVIEDMAGKFILNSE
                     SMRLLREKIEADRFEVARKILGVKTVEGYDALVLTKELALDRKGLFAIQKVFGGMPVI
                     ILADESGLSDLDRDFISRIPEASRPVFLSAALLKTPGDLSEAVRKSLRGKSSGRMMSL
                     KAMAGTTDAMATLLKRQLKDEVVILTTSMFENFLSVAGVGGLVEQMQAEYLAIVRSA"
     gene            6355..6519
                     /locus_tag="PK590_01700"
     CDS             6355..6519
                     /locus_tag="PK590_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23343.1"
                     /translation="MNYWIKWFLPGVLFIVTAFWAGTLPSLVFALAGFLSFFTIGVTL
                     IIEGRKRNKK"
     gene            6581..6973
                     /locus_tag="PK590_01705"
     CDS             6581..6973
                     /locus_tag="PK590_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23344.1"
                     /translation="MNYFQALSLIMGTGMLVGGLWVVFRPGAWQKLVFRVMSEKRPDW
                     VLGVNAAWTLLVLFTWGMFFGEMALASFVVTFVMSLTLLKVLGSVFFWKQYREAALTL
                     FREPLAMRTVMVSTAAIGFALLSLGLMV"
     gene            7258..9291
                     /gene="hyfB"
                     /locus_tag="PK590_01710"
     CDS             7258..9291
                     /gene="hyfB"
                     /locus_tag="PK590_01710"
                     /inference="COORDINATES: protein motif:HMM:NF005086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HOG23345.1"
                     /translation="MEIFFYAQILLHLLGGGVGFLFNPRGAHASRATHGIAVLASLAG
                     VVFSGNILLTGHGWFHEWRGSLPVGPLSLSADPFSALFILIISLLSLAASVFAIGYVR
                     EYQGRKNIASLGLFYHAFILAMLLVVTVQNAFYFLLFWELMSLASYFLVIFEHEKQEA
                     RSAGTLYLVMTHIGTAFIGTAFWLLFMHSGSYEFQAFRESAAALPLGLRNVIFFAALI
                     GFGTKAGVIPLHVWLPRAHPQAPSHVSALMSGVMIKMAIYGLWIFLFSFLGEGPGWWG
                     PVVLGVAILSTFGGVLYALMENDLKKLLAFSSIENIGIVLLGIGMALVFSSADQPLYK
                     GFALIAALYHVINHAMFKGLLFLGAGSVLSTTHTGNIENLGGLIRTMPWTAAGFLVGA
                     VAISALPPLNGFVSEWLIFISLLLGFESAEVTFHFFSPLLAALLGLAGALVATCFVKA
                     FGISFLGQPRSHHAAESRESGMSMLLGMGILAATCLGLALGAPWVFRILGETVETSSG
                     GFFSMEVFVQKIFFLSPSGFEAGRFSPLIIALVITVLLLGIVLLLRLFFGKASIRTGP
                     SWDCGMPGLAPRMQYTSTGFSQPFRRILSFLYQPTRRVELEDEGMKILRTAQRFESRI
                     TQHVDEWVYQPLIELVGRLSRKVKYIQTGHIQLYLSYIFVTLILLLLFFGGRA"
     gene            9291..10241
                     /locus_tag="PK590_01715"
     CDS             9291..10241
                     /locus_tag="PK590_01715"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOG23346.1"
                     /translation="MSAIILSLFQVIFLMVLAPAVSWFIKRMKAASQNRRGPTLVQVY
                     SDLGKLFRKDMVISETASWIFHLMPFVLFASTVAAALIVPVLGNHALLGFMGDAILFV
                     YLFALGRFFLALAALDAGTTFGGMGSSREMTLSSLAEPAMLLAFFSLGFRSHTLSLGG
                     MVSYFAGAEPFQVLFFTSLAFGALLIVTIAESGRIPVDNPATHLELTMIHEAMILEYS
                     GRYLALIEWGHQIKQLVFLTLLANLFLPWGIAGSWSFSALGLALVLYLAKVFFLAFVI
                     GWIEIHSAKLRLFRVPDFLSVAFVLAILSLLGQMIVGRGV"
     gene            10238..10897
                     /locus_tag="PK590_01720"
     CDS             10238..10897
                     /locus_tag="PK590_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HOG23347.1"
                     /translation="MTTETLASLIHFLGILLLISSFWILTSSQIRTSIRAYAAHSFFL
                     ALITGVIAIANHIEHLLISAILTFGLKAIVIPHVFFRMIRRLGIRKEVESYVNIPFTL
                     LLAVLLVFVSFYVTRDGSFLKPVAFGECIPLAIATVFFGMLMMTTRKKAISQILGLLL
                     IENGLFLMAVTMTFGMPLLVEMGIFFDLLVAVMIMGVFIFKIRDAFESIDTDEMTGLK
                     G"
     gene            10902..12377
                     /locus_tag="PK590_01725"
     CDS             10902..12377
                     /locus_tag="PK590_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HOG23348.1"
                     /translation="MTIEKVLGTPLVFAFLCLIPCSKRMTRVIFTAGAILAALGAWSF
                     VFGVSGSPRVFAFGGNLMMDSLSAVFVLLITGVGVLSSAYAGGYLFRDGETAIPSVKL
                     RRYAFFFHLFLFTMLLTVFSNNLGIMWIAIEGTTLASAFLVNTDDKKSSIEAAWKYLI
                     ICSVGISLAFFGVILAYYSAVARTAVGESGLLLNWTFLLENARQLDPKIIQLAFIFVL
                     VGYGTKAGLAPFHSWLPDAHSQAPAPVSALLSGVLLSCAVLGILRFHILTTAATGSNF
                     SGTLLVVFGVFSVMIAAPFILEQKDYKRMLAYSSVEHMGLAAVGLGFGSAIGIYGALL
                     HIVNHAFTKSLLFFTTGHILYLFQTKEISQVKGIFRRSPFLGAIFFAATLAIAGMPPF
                     NIFVSEFLILSAGFQAKKFVACGILLVLLSLVFIGFLKHSNQMTFGESEKVPVKPKSL
                     VMNGVLLVGMVLILMMGFYIPGPLQHLLQEASRVAAGGASL"
BASE COUNT         2588 a         2988 c         3595 g         3335 t
ORIGIN      
        1 ttgtgtggat ggtcactcac acctatcgaa accgcgggag cgttgattac gggaggatgg
       61 gttttgggac ttctgtttgg caccggcttt ttaagaatga cgcttatccg aaccccagcg
      121 attcgacaag cgctggcgtc cgctcggccg atggcgccag tgccgtggcc gcggtccacc
      181 gtcacgaggt tgctccgctg gatccggatt caaccgtggt cgcgattacc aacggcgatc
      241 gccgcgaaat ctcaagctct gtttttcgga aaattttcaa aaatcttttt ccgggggagg
      301 acccagaatt tatcactgcg gaacagatcg tttcggtcaa acgagccgcc aaggaaaaac
      361 tcgaagagga aatgcagtgg gggctggatc cggacaaaat tgtgatttca tactccggaa
      421 gaatggtgca ggagaaggcg ggccgtagcc gtgcgttcca cacccaaaat cttcgtgagc
      481 tggtgcggct cggcgcacag gtcattattt acggaaatgt tcagtccagc atagaaagcc
      541 gggagatgtt tgaagagctg aggcgcctct cggatgaatt gaatggagtg ggtcccggaa
      601 aactgattgt tcgcacgggc tggagcattg aagatcaaag aaagcttctt gccgcgaccg
      661 atctccaggt tcaggattcg gatcgtgacc gttatcgagg taccggagcg gctgaatata
      721 cggaagcgga cgtatcggtc aacggcggtc ttcagatggg gccggcatgg cttgagggca
      781 ttatccagaa acagggaata gtgcttgacc gtacccgccc cggttcggga aacacaatca
      841 tccccgcgag cgacaacgta actccggatg cggcgaatga tgatcccgta gcctacatga
      901 atgccttcag gtgggttatc gatactttta ataatgatcg aattcatttg gcgtcttacc
      961 aggcggcgtc cgtgcgctta agcagggttt tgaattctga tttgccggcg gccgaatacc
     1021 ttcgtcaatt tgaccggggg gctcgcaaaa aggatgcgct gaaggaactg gcccggcgga
     1081 tcgcggaagc gaagaaaggg ccgggttttc aggccgcgct ggacgaggcg cttgaaaaaa
     1141 taacccgaat ggatcgtaac gaagctaagg aggaatttgg cgccaataaa cttcccgcga
     1201 ttatggccgt ggtaacgatc ctgaagccgg agctgcttga ccgaatccgt ttgtggaacg
     1261 cccgggagca tgaagttctt tctgcgttga aaaatcatcc ggagatgaaa gatctttttg
     1321 aaaagggaat gaggcgtttt catcccattg tgatgcgcga tgaggagctt gcggatgaag
     1381 accggctggc ggtttcgttg agtctgggtg gcagaaatct ggttatggtc atggatcttg
     1441 cttttaacaa ttacagccag gaagaaggaa aaacctgggg gcggattgga gagatagagt
     1501 ctattgtttc cgacccaggg gcgcattacc gggtttttga ctggtcgttg gatccgggag
     1561 agaaggtaac ctatcccagc gaacacttcg ggcagaaaat gatgagcgac tggatggtcg
     1621 gcattcccgt tcgggacaga gagcgcgacc gcaacggttt accacaacgg ggcaaaagtt
     1681 accaggccct tagcgtgtct gtcgtccgtt cggaggcgag ggcatcacag ctggatttgc
     1741 cgggcctgcc tttggagatg gaggctttgg ctgagcgctc cgtgacgttt atcgacaggg
     1801 atatgagcgt ggaaaccgtc gccgagcgta ttcaaaataa ccaggtcatt tatctggcgc
     1861 ctccgcttga ggtgggctat gactacggaa aatatgaaga acagctggtc cagatggcgg
     1921 tcacaaagat cggtccggcg ctcgaaagag ttcgccgcca tgaggcgggc ataagaaagc
     1981 cttacgagcg aaaagttttt atccttcgcg gcatggtcga ccccggcact tcaaaagccg
     2041 tttacgacgc cattaaatgg aacgcccatc cgcccaaggg cgatgattat gattttgacc
     2101 tcgtgttcca accggatttt acgtttctgg acgcgcggac ggaaaaaacc gaagaaccca
     2161 ttgtggtttt tggcgtgatg ggggaagacg acggaaagga cgccgctttt caggcggcca
     2221 caaaaacagt cctggaccgg attttcgggc agttctatca ggggaaaaag attcagtaca
     2281 tgcgcattcg tagcgccgag atgtccaagg aaatgctcat catttacctg ggggctaaac
     2341 tggcccactt tgacgatgtg gccgagcttt cccgggctta cggggccgac ttaagcgccg
     2401 ccgcgttcgg ggccggcctt gataaacgca tccgcaccat tttcacaaac ccctcattgg
     2461 ggtttggcgg caggctcttt gtctacttgc attggattta cgcccagcga ctttataaag
     2521 cggccgcgga actcgaagga cagacgcacc agaaactgaa tgaccggaaa atggaaatcg
     2581 aacaaaaaat ccgttccgcg gtcgagcggc tggagcgcgg cgaaaacatc acggagatcg
     2641 tcaaggattt gccgccgcaa cttcatctgc tctttaccat aaagacgatt ttgaagatca
     2701 ataaacagaa tattctggat ttccttcggt cgatagaacg cgcttatgaa agcatgtatg
     2761 ggagtgggtc cctgcgcgga aagaaagtgg cgctcttagg cgttggttac agttctcaaa
     2821 gtgggcagat caccgcctca ccggccctgc tccttatcga acggttgatc gtcgatgagg
     2881 ggatcagcga gtttgtgatc gcggaccctg acgcgaaagc cgcttttatg aaatgggtgg
     2941 aagaaaagcg cggggaaaat ccgcgcttta acgccgtgaa atttgaattc accgataata
     3001 tttatgacgc cgcgaaggtt tcggatctaa cgatcattcc gacagattcc aacaaagccc
     3061 tccgggatat cgatatcaaa cgtttgggcg acgcgcttca gggccgcccc gtgtttgacg
     3121 gcatgaatat tttcggactc cgggccgacg gcacttaccg gtataaactg gaagacgtac
     3181 gcgatgccgg catcaacctc gtgggggtcg gccggttacc gcttggaccc gaattggacg
     3241 ctcaaaccgg ctatcggctt ggggacagtg ttgtggtccg tgatgttgcc ggataccggc
     3301 aggttctcca ggaaagaggc gggtctgtta cagcgcaaca aaagtcggtg actgtgatcg
     3361 gaggcgggta tgtggggctt gtgacggccg ccaatctggc agggctcggt cacagggttc
     3421 gtgtggtgga catcaaagcc aagcaaaaag aaatggatgc cctgaacggc ccagaaaccg
     3481 ccgttcccat ctatgaaccg ggtcttcgcg aaatgatcat tgatgggaag gcgaagggtt
     3541 tgatcacgtt ctcgacggac cttgagccgg cggtccaggc ggcttcgatc gtttatcttg
     3601 cggtggggac tcccgcgcag gatactggcg aagttgattt gaaatatatc ctgcaagcat
     3661 ccggggatat cggggaggcg atcaaaaaac atggcggagc gaaaacgctt gtcgttaaat
     3721 ctaccgtgac gccggacacc ttcaagaaaa tggacgagac gcttgccgcg aaagggctta
     3781 aactcgggaa agattacgcc ttggtcagca atcccgaatt ccttcgcgaa ggccaggcca
     3841 ttcaggacgt gacccaaccc gaccgcacgg tccttggttt ctacgcggcc atgagtctgg
     3901 aagaccgtaa acgcgcggaa aaagaaattc tcgagctgtg gtatcccttg atgctgaaaa
     3961 aagaacacac ggtccttttg acggataccg cgagctcgac cattgtcaag tacctggcga
     4021 acagttttct ggccatttct atcacgcttt ccaatctttt ttcctccgcc gcggaagcgg
     4081 atcacgccga ttttatggag gttcgtatgc cgtttaaggc cgatgaccgg atcggtggca
     4141 acgcgttcct gaatgccggg gtcggatacg gcgggtcttg cttccccaaa gatgttttgg
     4201 ctctcaactt cagctccaag gacgcgacag gacatgtgct tcccctgatc gatatcgcga
     4261 cgggctttaa tgaatatttt aaaacggcag tggtggcacg cacggtcgaa agcgttgccc
     4321 ggttcccgca agccgcccgg ccgctggagg ggaaaagcgt cattatgctt ggcatggcct
     4381 tcaaggcgga caccgatgat atgcgcgaag cttcaagcgc gtccgttctt catgaattac
     4441 ttgtcagggg cgcgacgcaa gtacgccttc acgatccgat cctttccctg ccgaatgtcc
     4501 cgccgaggga agttatcctg gatcattttc tggatactct ttacaagatc ttccgggacg
     4561 gggaagaatt tcagaaggct tatcagcaac acaaggccct ttggattgcc cggggggaac
     4621 cggtggacgg tgaagagaag aactatttca agaggactta tttcccggag aaattcatta
     4681 agaccggacg cgtggtattt gagccggatc ttgacaagct gggaaaggcg gacatcgttt
     4741 ttctcgtgac ggaatggcgc cagtacaaag gacttgatct ttcccggttc aaacagacag
     4801 accggaaact ttgggtggtc gacggacgga atctttttta cgaccgtcgt gacgagatca
     4861 gccagtttgc cgagtatcgt ggcatcggga cggccttttt cggcccccgt tctgaagcgc
     4921 gtgaggcctt tgccgaagtt ggcaggatga ttacgactaa aaaacttagg cttccgaaat
     4981 gggttggaga ttactcgaag gccacgcgga atcgtgtgtt tgcaactccc gaagaacgga
     5041 tgagtgtcgc cgtggagctt tcgcggctta atgtggaaaa tgggactggc gggcctttcg
     5101 gggcggtggt ttttgaccgg aagtccggaa agattttttc gatgggtgtg aatctggtgg
     5161 tttcggaaag agcctcttat gcgcacgcgg aaatggtggc cttgaccctg gctcaggaac
     5221 gtttgaaaaa ttttagtctc gatcatgacg gaagggagtt tgaactggtg acgagttctc
     5281 aaccctgcgc gatgtgtttt ggggcgatcc cgtggtctgg agtcaaaggg gtcgtggtgg
     5341 gcgcccgagg ggaggatgtt gaaacgatca cgggttttga tgaggggcca atccatcctg
     5401 aatgggataa agagcttttg cggcggggga ttgacgtgaa acgtgatatt ctcagggata
     5461 aggcgatcga ggttctccac gcttatgtgc gaaagaaagg aacgatttat aacggcaagg
     5521 ctgaatctga aaaatcagta cgttcggaaa cgcgcgtcta ttcaggcctc gagtttcaac
     5581 agattcctga aatgaattat gaggaatttt tggcctggta cgaaacgcaa agcaaggctt
     5641 cgcggaccgc gatggaaaat ctgactgtcg aaatcatgca agggatcgcg ccgcttctgg
     5701 ccaaccggtt taaagtcatc gaggatatgg ccggtaaatt catcctgaat tctgaatcga
     5761 tgcgattgtt gcgcgagaag attgaagcgg atcgttttga ggttgcccga aaaattcttg
     5821 gagtgaaaac ggtcgaaggg tatgacgcgc ttgttctgac caaagaactg gcgcttgacc
     5881 gcaaagggct tttcgcgatc cagaaggttt ttggcggcat gccggtgatt attctggcgg
     5941 atgaatccgg tctcagcgat ctggaccgcg atttcatttc ccggatcccc gaggcgagcc
     6001 ggcccgtttt tctgtccgcc gccctgctga aaacccccgg tgatctttcc gaagcggtga
     6061 gaaaatccct gagggggaaa agcagcggac gcatgatgag tctgaaggct atggccggca
     6121 ccaccgatgc aatggcaacc cttttgaaac gacagctcaa ggatgaagtg gtgatcctga
     6181 cgacgagcat gtttgaaaac ttcctgagcg ttgccggcgt gggcggtctt gtggaacaga
     6241 tgcaggccga gtacctcgcg attgtccgtt ccgcgtaaga ccagtccctt ctcccggttt
     6301 tcaaatactc tttttgcgct gtactctctt cggtgagatc cttacaataa ggccatgaat
     6361 tactggataa aatggtttct ccccggcgtc ttgtttatcg tcaccgcttt ttgggcgggg
     6421 actcttccca gtctcgtctt cgcgctcgct ggttttcttt cgttttttac gatcggggtt
     6481 accctcatca tcgaaggtcg caaaagaaat aaaaaataat cggatcccgg gtaaattttt
     6541 aagccacaat ccgcgttttt cagtcgaaag ggatttaaaa atgaattatt tccaggcgtt
     6601 gagtctgatc atgggaaccg ggatgctcgt gggcgggctt tgggttgtgt ttcgcccggg
     6661 agcctggcaa aagttggtct tccgggtgat gagcgaaaaa agaccggact gggttctcgg
     6721 cgtcaatgcc gcgtggacac tgctagttct ttttacctgg ggaatgtttt tcggggagat
     6781 ggccctggcc tcttttgtgg tgacgttcgt catgtccctg acgcttctta aagttttggg
     6841 gagcgttttt ttctggaagc agtaccggga agcggccctg acgctgttca gggagccgtt
     6901 ggccatgagg actgtcatgg tctcgaccgc cgcgatcggc ttcgcgcttc tgagccttgg
     6961 gctcatggtt tgaaaccggg cgccacggga ttttttttaa aaaacgtccg gcaatccttg
     7021 aacagtgtcg gaaacaagac cccataaaga gccgcacaaa aacaggaatt tatggtatca
     7081 tgcggcccct tccgggttgc ccccgaagaa acctcggcca gcgggataaa aacttaaagg
     7141 gcgattatct cacgcaagat tttgcggaaa gggcgggcgg ggattttttg tgtcggggcc
     7201 ttatgatgtt ttaaatcctg caagtttgct tgcaagtttt gaggattgtt caggaccatg
     7261 gaaatatttt tttacgctca aattctcctc catttattag ggggaggagt gggttttttg
     7321 ttcaatccgc gcggcgcgca cgcgagcagg gcgacgcacg ggatagccgt tttggcgagt
     7381 ctcgcgggcg ttgtcttttc gggcaatatc cttttgacgg gacatggctg gtttcatgaa
     7441 tggcggggca gtctgccggt cggcccctta agtttatccg cggatccctt ttccgctctt
     7501 tttattttga tcatatcact cctttctctc gcggcctctg tttttgccat cggttatgtc
     7561 cgggaatacc aaggcaggaa aaatattgcc tcgttgggtc ttttttacca cgcgtttatt
     7621 ctcgcgatgt tgcttgttgt caccgttcaa aacgcgttct actttcttct tttttgggag
     7681 cttatgtccc tcgcctctta ttttcttgtg atctttgaac acgaaaaaca ggaagcgcgt
     7741 tccgccggaa ccctctatct ggtcatgacg catattggta ccgcgttcat cggaaccgcc
     7801 ttctggcttc ttttcatgca tagcggttct tacgagttcc aggccttcag ggaatcggcc
     7861 gctgcacttc ccttaggtct caggaacgtt attttttttg ccgcgcttat cgggttcggg
     7921 accaaagcgg gcgtgattcc tctccacgtc tggttgccgc gggctcatcc ccaggcgccc
     7981 agccatgtgt cggcgctgat gtcgggggtt atgatcaaga tggcgattta cggcctttgg
     8041 atatttcttt tcagcttttt gggagaaggt ccgggatggt ggggaccggt cgttcttggg
     8101 gtcgctattc tttcgacgtt tgggggagtc ctttacgctc tcatggaaaa tgatctgaaa
     8161 aaactgcttg ccttctccag tatcgaaaat atcggcattg tccttctggg catcggaatg
     8221 gccctcgttt tttcctcggc tgatcagccg ctctacaagg gttttgccct gatcgcggcc
     8281 ctgtatcacg taattaacca tgccatgttt aaaggacttc tgtttctcgg cgcggggtcg
     8341 gtgctgtcaa cgacccatac gggaaatata gaaaatcttg gggggcttat tcgcacgatg
     8401 ccctggaccg cggccggttt tttggtcgga gccgtggcga tttccgcgct tccgccgttg
     8461 aatggttttg tcagtgaatg gcttattttt atctcgcttc ttttggggtt tgaaagcgcg
     8521 gaagtgactt ttcatttttt ttcgccgcta ctggccgctc tcctcggttt ggcgggcgcg
     8581 ttggtcgcga cctgtttcgt gaaggctttt ggtatcagct ttttgggtca accgcgatct
     8641 catcacgcgg ctgaatcgcg tgaatcggga atgtcgatgc tactgggaat ggggatcctc
     8701 gcggcaactt gtctgggtct tgctctggga gctccctggg ttttcaggat cctgggggaa
     8761 acggtcgaga cgtcttccgg ggggtttttt tcgatggaag tttttgttca gaagattttt
     8821 ttcctgtctc cttccggttt cgaggccggt cgtttttctc ctttgatcat cgctcttgtc
     8881 atcacggttt tgcttttggg gatcgttctt ctgctccgcc tctttttcgg gaaagcatca
     8941 atccggacgg gcccaagctg ggattgcggc atgccgggcc ttgcgccccg aatgcaatat
     9001 acgtcgacgg gtttttccca gccatttcgc cgaatccttt cttttcttta tcagcccacg
     9061 cgtcgggtgg agctggagga tgaagggatg aaaatcctga ggaccgcgca acgctttgag
     9121 tcgaggatta cccagcacgt ggatgaatgg gtctatcaac cgttgattga acttgttggc
     9181 cgactttcac ggaaggtgaa atatatccag accggacata ttcagcttta tttgagttat
     9241 atttttgtca cgctgattct cctgctcctg tttttcggag gacgcgcctg atgtcagcga
     9301 tcattttgtc ccttttccag gtgatattcc tgatggtttt ggcgcccgcg gtgagctggt
     9361 tcatcaagag gatgaaagcg gcctcgcaaa atcgcagagg gcctactctg gtacaggttt
     9421 attcggacct cggaaaattg ttccggaagg atatggtgat ttcggagacc gcgtcctgga
     9481 tttttcatct catgccgttt gtactttttg cttccacggt agccgcggcc ctgatcgttc
     9541 cggttttggg aaatcacgcc ttgttgggtt ttatggggga cgccatcctc tttgtttatc
     9601 ttttcgcgct gggccgtttt tttctggcgc tcgcggcgct ggacgctggg acgacgtttg
     9661 gcgggatggg ctcgagccgg gaaatgaccc tttcgtctct tgccgaaccg gccatgctcc
     9721 tcgccttctt ctcattaggc tttcgatctc acaccctgtc cctgggcgga atggtttcat
     9781 atttcgccgg ggcggagcct ttccaggtgt tgttttttac gagccttgct tttggcgcgc
     9841 ttttgatcgt gaccatcgcg gaaagcggaa ggattccggt cgataatccc gccacgcacc
     9901 tggagctgac catgattcat gaagcgatga ttctggagta ttccgggagg tacctcgccc
     9961 tgattgaatg ggggcatcag atcaaacaac tggtgttttt gactcttctc gcgaatcttt
    10021 ttctcccctg gggaatcgcc ggctcgtgga gtttctccgc gctggggtta gcgcttgttc
    10081 tctatctcgc caaagttttt tttctggcgt ttgtcattgg gtggattgaa attcattcgg
    10141 cgaaattgcg tcttttcaga gtaccggact ttttgtcggt ggcgtttgtt ctggcgattt
    10201 tgtcgctgtt gggacaaatg attgtcggga ggggcgtatg accacggaga ctctggcgtc
    10261 cctgatccat tttctcggca tccttctttt aatctcttcg ttctggattc tcacttcttc
    10321 ccagatcagg acgtcgatcc gcgcctatgc cgcgcattct tttttcctcg cgctcattac
    10381 cggggtgatc gcgatcgcga atcacattga acacctgctg atttcggcga ttttaacctt
    10441 cggcctgaag gccattgtca ttccgcatgt ttttttcagg atgatccggc ggctcgggat
    10501 ccgtaaagaa gtcgaaagtt acgtcaatat tccttttacg cttcttctgg ccgtcctgct
    10561 ggtatttgtt tctttttatg tcacgcggga cggcagtttt ctgaaaccgg tcgcctttgg
    10621 cgaatgtatc cctttggcga tcgcgaccgt ttttttcgga atgttgatga tgacgacccg
    10681 gaaaaaagcc atcagccaga tccttggatt gcttttgatc gaaaacggtc tttttttaat
    10741 ggcggtgacg atgaccttcg ggatgccgct tttggtcgaa atggggatct ttttcgactt
    10801 gctggtggcg gtcatgatca tgggtgtttt tattttcaaa atccgcgatg cttttgaaag
    10861 catcgacacg gacgaaatga ccggtttgaa aggatgatgt gatgacgatc gagaaggtat
    10921 tggggacgcc ccttgttttt gcctttcttt gcctgatccc gtgttccaaa aggatgaccc
    10981 gcgtgatttt tacggcgggg gcgatcctag cggccctcgg ggcctggagt tttgtttttg
    11041 gagtgtccgg atcgccccgt gtgttcgcgt tcggcggcaa tctcatgatg gattcacttt
    11101 ccgcggtgtt tgtgcttttg atcacgggcg tgggagtgct ttcgtcagcg tacgccggag
    11161 ggtatctttt cagggacggc gagacggcta ttccttccgt gaaactccgc cgctacgcct
    11221 tcttttttca tctctttctc ttcaccatgc ttttgactgt tttttccaat aatctcggaa
    11281 ttatgtggat cgcgattgaa gggacgactc tggcctccgc ttttctggtc aacacggatg
    11341 acaaaaaaag ttcaattgaa gcggcctgga agtacctcat tatttgcagt gtcggtattt
    11401 cgctcgcgtt ttttggagtg atcctggctt attattcggc ggtcgcccgg acggcagtgg
    11461 gagaaagcgg cttgttactg aactggactt ttttactgga gaacgcccgt cagctggacc
    11521 ctaaaattat ccagctcgcg ttcatttttg tgctcgtggg ttacggcacc aaggcgggcc
    11581 tcgcgccttt tcattcctgg cttcccgatg cccacagtca ggctccggcg cccgtgagcg
    11641 cgctactttc cggcgtgctt ttgagttgcg cggtcctggg tattttgcga tttcatatat
    11701 tgaccacggc ggcgacaggc tcgaactttt cggggacgct actcgttgtg ttcggggttt
    11761 tttcggtgat gatcgcggcg ccgtttattt tggaacaaaa ggattacaag cggatgctgg
    11821 cttattccag cgttgagcac atgggtctcg cggcggtggg gcttggtttc gggagcgcga
    11881 tcggaattta cggggccctg cttcatattg tcaaccacgc gttcaccaag tccctccttt
    11941 ttttcaccac gggacatatt ctttatctgt ttcagaccaa ggaaatcagc caggtgaaag
    12001 gaatttttcg gcggagcccg tttctggggg ctattttctt tgccgccact ctggcgattg
    12061 ccgggatgcc tcctttcaat attttcgtca gtgagttttt aattttgagc gcgggatttc
    12121 aggcgaagaa attcgtggcc tgcggaattc tcctggtgct gttgtctctc gtctttatcg
    12181 gttttttaaa gcattctaat caaatgacgt tcggcgaatc tgaaaaagtt ccggtgaaac
    12241 ctaaaagtct cgtgatgaac ggggtgctcc tggtgggaat ggtcctgatt ctcatgatgg
    12301 gtttttatat tccggggccg ctccagcatt tacttcagga agcaagccgc gtggcggcgg
    12361 ggggcgcctc tttatgaaga cggtcatcga cgggctcaaa gggaaattcg aaaacaagat
    12421 tcacaaggtc tgggacgggt gcgggaagca ggtgcatgtt agcgtggacc gggtgtttct
    12481 ccttgagatc gtggcgtttc tcgtga
//
LOCUS       DAOSIO010000023        12456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_5095_length_12456_cov_4.058705, whole genome shotgun sequence.
ACCESSION   DAOSIO010000023 DAOSIO010000000
VERSION     DAOSIO010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 12456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12456
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5095_length_12456_cov_4.058705"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..1444)
                     /gene="cysS"
                     /locus_tag="PK590_01730"
     CDS             complement(26..1444)
                     /gene="cysS"
                     /locus_tag="PK590_01730"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOG23349.1"
                     /translation="MGIKFHDTLSGQLKDFQAGDTGEVSLYTCGPTVYHYAHIGNFRT
                     FVFEDLLRRFLESEGYKVRHVMNITDVDDKTIAGAQREKKSLREYTSFYTDSFLEDRK
                     SLNILPADVMPNATDEIPAMISLIETLMTKEVAYKAEDGSVYYRVAEFPGYGKLSKKK
                     LDGNIAGVRVNQDEYEKEEGADFVLWKAAKENEPSWDSPWGKGRPGWHIECSAMCMRH
                     LGETVDIHAGGEDLVFPHHENEIAQSEAATGKPFARYWLHARHLLVNGEKMSKSKGNF
                     FTLRDLLQKGYDPMAIRYVLMSVHYRQQLNFTLESLKEAREVIEKLDNCYFQCLSRIR
                     IAGQKNGGELNLGASAAEIGKALGDDLNIAKALAIFQKTVSEINVHLAHLSETGLSAC
                     VEFFERMDRIFGFDISSVREIPSDVMRTLSEYVEARKVKDFATSDQLRKAIAEKGWQV
                     KDGRPGEPSTVKKNRRGWDGKK"
     gene            complement(1461..2723)
                     /gene="ispF"
                     /locus_tag="PK590_01735"
     CDS             complement(1461..2723)
                     /gene="ispF"
                     /locus_tag="PK590_01735"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOG23350.1"
                     /translation="MRHKHKGYHVPTISLIIPAAGLGTRFHQSSGAAFSKSRRRDSKL
                     FYDLAGEPVLARTLAAFAEIPEIREVVLAVSPGMRSEIKRVLGRAACQKIRYVTGAKT
                     RAGSVLSALRRTSPSSEWVMVHDGARPLVTTAAIRQLIRGSVGWDGVILARKVIPTIK
                     RVLRSDGRIESTVDRSTLYEAETPQMVRRSVLIKAYKMNPCALQATDEAGLLESIGAR
                     VRVISHDGWNPKITTVTDLFLAESMIQNSNPTCTRTGIGFDTHRLVPGRKLWLGGIAI
                     PSPKGSLGHSDGDALLHAIADAMLGVIGRGDIGEWFPDKDPRWKDVKSGVIVEKVLAE
                     SKKAGWLPYHVDSNIILETPRLGPYKMKIRRRIARLLQLPVEDVSVKARTREGLGAEG
                     EGLAITCQAVVSMKRIQIAEGDIRKWRS"
     gene            complement(2813..3988)
                     /locus_tag="PK590_01740"
     CDS             complement(2813..3988)
                     /locus_tag="PK590_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOG23351.1"
                     /translation="MIIFIRLLFLAGCATFGYLAPFSWLDGLNFLDRNWLSAAICLMG
                     GGVFVLLDIFFEQYTVKNILSIILGLAIGLLTHKLFMMVVAYGNFAPEAERTFAILTA
                     VLFSYLGIITILRGQDEFSLIIPFVRLDTKGAKEDLILLDTSVIIDGRIADIANTHFI
                     GGKLILPRFVLKELQLISDSSDPLKRNRGRRGLDVMNAIRENPNVTVKIHEMDFPEFN
                     TVDAKLVKMAQVIEAKVFTNDYNLNKVAELQGVKVLNINDLANALKPVVMPGEEMEIK
                     ILKEGKEHEQGVAYLDDGTMVVVDAARRRIGQTTKVTITSVLQTQAGRMIFGKLSDED
                     EGRRDRHDRGRGDRWHENRERGRGDRHERNGGRNGHHHQPGGQSVNQGQGPATPPNH"
     gene            complement(4138..4950)
                     /locus_tag="PK590_01745"
     CDS             complement(4138..4950)
                     /locus_tag="PK590_01745"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOG23352.1"
                     /translation="MKKSANEMTVGIFVIIGFILLTLTLFFVSGVYLFRSGYHVKVRY
                     EYVSILNKGAPVRMAGVRVGEVSQVRLYFDETKKKNCVEVKLFIEKGIEIRENYTFKI
                     QGTHVLSEPHIEISPVAGDARLLKGDDMVEGISPVPVEALIDRAHEISDEISEMMKKF
                     RANVEDEGMIADLKTMVKNFSELSVALNTSVSGSEKELSQSVKNIEASTASLERILGQ
                     VESGEGTLGKLLMEDEIYNEMRAFVSDIRAHPWKLLKKDSGNSRKWYYLFLK"
     gene            complement(4947..5717)
                     /locus_tag="PK590_01750"
     CDS             complement(4947..5717)
                     /locus_tag="PK590_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG23353.1"
                     /translation="MIEVRNIHKQFGDKDVLRGVDLTVRDGETLVIIGRSGCGKSVLL
                     KHIMALLEPDEGDIKIDGRSMFSMTPDEQDRFRLKLGMLFQGAALFDSLTVRENVGFS
                     LYEHTRFSEEAIEMKIREKLSWVGLSNIEDLMPSSLSGGMKKRVGLARAICHEPSIIL
                     YDEPTTGLDPINADVINELILQKQRRLHVTSIVVTHDMTSAFKVADRIAMLYEGKIIA
                     TGTPHQIRNSKDPIVQQFVTGSATGPITQGRKKETEIL"
     gene            complement(5730..6542)
                     /locus_tag="PK590_01755"
     CDS             complement(5730..6542)
                     /locus_tag="PK590_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014425740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG23354.1"
                     /translation="MSRWSYLKTLVSVRLWLENFGAGCLNFFYHAGGIAILFRDTCVL
                     IFRRSIRWPMVWDQMLKIGVTSLPLVFLTALFTGMVLALQSAYQLRLFAAEQFTSDLI
                     SVSLCRELGPVLTAMVVAGRVGASIAAELGTMKVTEQIDALKALATNPIQYLVIPRFI
                     AGVCMLFMLTIYADCIGMLGGYLIAVFKLGMSSHMYIKRGFDALVVKDIITGLIKAFF
                     FGGIITVVGCYFGFKARDGAEGVGRATTLAVVVSLILVIASDALFTAIFYFF"
     gene            complement(6550..8478)
                     /locus_tag="PK590_01760"
     CDS             complement(6550..8478)
                     /locus_tag="PK590_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23355.1"
                     /translation="MKKHSFLKGLILLLLFFLIAVVLAAKVFLHQFATSEEFKRFAES
                     RAGEYLRAKVSIGQIRPYHFNQLALENVIIELPEIRGNSQLIHLDRLLFRYHLSHLWK
                     GKFDAPAGVTFRNPSLMIEEDKFPYQYFETAASAPGGFGMPTIDFKGGEIRYRVSSLG
                     KDIVLRNIEGTITAEEGTKARVNIRATASGILVGGVDVHGTVDAFQKTHDLWLDLKGM
                     DFSSEIPIPLRGLTGKLHWSGKNLSFEQVQTTLHGWNAKVSGAFVNRDGRPETTVRLV
                     VGKERPWMEAGFKIDMSKEFMEGFLDASDAGKFSFNGKIRSEGNRWILDPLTLNDHYQ
                     GKCELDFVTGNYEIAVEREMKRISIHSNLCRLDFAVQGRLDHLDFLGLDIVTQGRIYI
                     HAISPNWQDRQFRFKADFETDYFILNQEPFHDLKGAFEVTPYGISGIRCTWGRKFEMT
                     GQMVFPLKSPQMKLIVRTADFDLGQVQFFASKPLPKEMGGKLGGKLLIEGDLKRPEII
                     GVFNVSDGKWGKLDYDRGIIQFRGFAPYFPLQESKIWKGRSVFFLTGAVDLTLDNVMA
                     GVKIETLDRLVVWKGLEVTLHEQEANVQLAGSDLDHLKPVSSIEVGPVKQFESKTGEP
                     EESRPDEKGFTVGTSLKF"
     gene            complement(8486..10954)
                     /locus_tag="PK590_01765"
     CDS             complement(8486..10954)
                     /locus_tag="PK590_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOG23356.1"
                     /translation="MFDRFTERARKVIILAKEEAKRFNHDYIGTEHILLGLIKEGESV
                     AAAVLQNLGLSLDTIRLEIEKLVQFGPSTVVSGDIPFTPKAKKVIELALDEARRLGHN
                     YIGTEHLLLGLIKEGEGVASHVLMNIGLDLNKVRAEVIKLLGSATPGSDSAAMSGGAQ
                     ASGMGSGGSKSKTKTPALDAFGRDLSQLAREGKLDPVVGRNDEIERVIQILARRTKNN
                     PVLLGEAGVGKTAIVEGLAQRIIAGDIPEILLDKRMIVLDLALMVAGTKYRGQFEERI
                     KAVMDEIKRADNVIIFIDELHTLVGAGGAEGAIDASNILKPSLSRGEIQCIGATTLDE
                     YRKYIEKDAALARRFQTVMVDPPSVDDTVQILKGLRDKYEAHHRVKITDEALSEAAKL
                     SDRYITGRFLPDKAIDIIDEAASRARLSVTTLPKDLKKMETEIESLRREKEASIKGQD
                     FEKAAKLRDEERKAKEELQKMKKNWKESKSEVEIQVTAEDIAFVVSKWTGVPLARLEE
                     KETARLLKMEEEIHQGVIGQDEAITAIANAVRRSRSGLRNPRRPIGTFIFLGPTGVGK
                     TLLARALAEFMFGDRDAVITVDCSEYSEKFNVSRLVGAPPGYVGYEEGGQLTEKVRRR
                     PYSVVLLDEIEKAHPDMFNILLQLMEEGRLTDSLGRKVDFRNTIVIMTSNVGADLVRK
                     QGDLGFGVRTDDMNYDSLKTKLLDETKKVFRPEFLNRVDDIIVFHPLSRQDLEQIIHI
                     ELGEVKDRLKEKAMELLLSDEVLKFLIDQGYDPVFGARPLKRTIQRYVENVLAEEILS
                     GKFQEGDKIQAEIRGDRVVFEKAT"
     gene            complement(11031..>12456)
                     /locus_tag="PK590_01770"
     CDS             complement(11031..>12456)
                     /locus_tag="PK590_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOG23357.1"
                     /translation="QVKKEFLQETSVEKVRELENRIQTLEDQNRAWQNEKQILTEEKA
                     YLDSVRLFAGQQIPEDLVTKMPSPQDLEGTLSFLGSKLKENFSRVHAIDLEIRENGKK
                     LVALRRELGEISGGRQKTRQIVTVDVEVSRAGHLDLEVSYKVSGASWSPVYDARAQFD
                     KSETELVTYAIVSQGTGEVWEDVDMTLSTAQVSVGGTMPEVNSWFVHPFQPRQPLRGA
                     SMKLRAKSLGMADMAMRSGGEAEEPMLATNMALEAENLYAQAAQKGVSVVYKLTRKVT
                     VRPDGSDQKVPVTAQILSSSFEYSAYPRMVPLAYLTSRVTNAKDLQLLSGPVNIFLDG
                     DFVGNSYVQNIAPGEEFDLYLGVDEGVKVKRELLEKKSDDVLIANIPSPNRKVTYKYK
                     LTAENYKAQAGKLNLFETIPVSQDERIKVKVSQVSQDPQDKDWKDRKGVWRWQLQLAA
                     GGKVEIFYTVVVEFPRDMTVEGLE"
BASE COUNT         3065 a         3655 c         2713 g         3023 t
ORIGIN      
        1 tccccgcttt aaaaccggaa tccgcttatt ttttcccatc ccagcccctg cggttttttt
       61 tgaccgtcga aggctcgcca ggacggccgt ctttgacctg ccagcctttt tcagcgatag
      121 ccttccgaag ctggtccgag gtcgcgaagt cctttacttt tctggcttca acatattccg
      181 aaagtgttcg catgacatca gagggaatct cccggaccga tgaaatgtca aatccgaaga
      241 ttcgatccat tctctcaaaa aattccacgc aagcggatag tcccgtctcc gaaagatgcg
      301 cgagatgcac gttgatctcg ctcacggtct tttgaaaaat agccagggcc ttggcaatat
      361 tcagatcatc ccccagggcc ttcccgattt cagccgcaga agcgccaagg ttcagttccc
      421 cgccgttttt ttgcccagca atcctgattc ggctcaggca ctgaaagtag cagttgtcca
      481 gtttttcgat gacctcgcgg gcttctttca ggctttccag cgtgaagttg agctgttgac
      541 ggtaatggac cgacatcagg acataacgga tcgccatcgg gtcataccct ttttggagaa
      601 gatcgcgaag cgtgaaaaaa tttcccttgg atttggacat cttctccccg ttgacgagaa
      661 ggtgcctggc gtggagccaa taacgggcaa aaggtttgcc ggtcgcggcc tcggattggg
      721 cgatttcatt ttcgtggtgc gggaacacaa gatcctcgcc tcccgcgtga atatccaccg
      781 tttccccgag atgacgcatg cacatggcgg aacactcaat gtgccagccc ggccgccctt
      841 ttccccaggg cgaatcccag gaaggttcgt tctctttagc ggccttccaa agcacaaaat
      901 ccgccccctc ttctttctca tactcatctt ggttcacgcg gactcccgcg atatttccat
      961 ccagtttttt cttggaaagt ttgccataac cgggaaattc ggcaacccgg taatagaccg
     1021 aaccatcttc cgccttgtac gcgacctcct tggtcatcag ggtctcaata agagaaatca
     1081 tcgccggaat ttcatcggtg gcattcggca tcacatcagc gggcaggata ttcagagatt
     1141 tcctgtcttc cagaaaagaa tccgtgtaaa aagaagtgta ctcccgaagc gatttctttt
     1201 cacgctgggc gcccgcgatc gttttatcat cgacatccgt gatattcatc acgtgccgga
     1261 ctttataacc ctcagactcg agaaaacgac gcaacagatc ctcaaacaca aaggtccgga
     1321 agttaccgat atgcgcatag tgatagaccg tcggaccgca ggtataaagg gagacttcac
     1381 cggtatcgcc ggcttgaaag tccttaagtt gtccggataa ggtgtcgtga aattttattc
     1441 ccatttgaaa tgaccgtcct ttagctacgc cacttgcgaa tgtcgccttc cgcgatttga
     1501 atccgtttca ttgaaaccac cgcctggcaa gtaatcgcca gcccttcgcc ttccgctccc
     1561 aacccttcgc gagttcgagc cttgaccgaa acatcctcaa ccggaagctg tagtaaccgc
     1621 gcgatccttc gcctgatttt catcttgtac ggcccaagcc tgggagtttc aagaatgatg
     1681 ttagaatcga catgatacgg aagccagcct gccttttttg attcggccaa tactttctcg
     1741 acaatgacac cgctttttac gtccttccag cggggatctt tgtccgggaa ccattcacca
     1801 atatctcctc ttccgatcac gcctagcatc gcgtccgcaa tcgcatgaag caacgcgtcc
     1861 ccatcggagt gtcccaacga ccccttgggc gatggtatcg cgatccctcc gagccataat
     1921 ttgcggcctg gaaccagccg atgcgtatca aaaccaatcc cggttctggt acacgttggg
     1981 ttgctgtttt gtatcatgga ttccgccaga aagaggtcgg tcacggttgt aattttagga
     2041 ttccatccat catgagaaat gacccggact cgggctccaa tcgattccag aagtccggcc
     2101 tcatcagtcg cctgtaaagc gcaaggattc atcttatacg ctttgatcag cacggaacgg
     2161 cgcaccatct gcggcgtctc cgcctcataa agcgtgctcc tgtccaccgt cgactcaatg
     2221 cgcccgtcgg acctcaaaac acgcttaatc gtcggaataa ctttcctggc caagatcact
     2281 ccgtcccagc caacgctccc ccggatcagt tgccgaatgg ccgccgtcgt gacgagcggt
     2341 cgcgcgccat catgaaccat tacccactct gacgacgggg aagttcgtct aagcgcgctc
     2401 aaaaccgatc cggctcttgt cttcgctcca gttacataac gtatcttctg acaagcagcg
     2461 cggccaagaa cgcgttttat ttcagaacgc atcccgggac taaccgccag aaccacttcc
     2521 ctgatctccg gaatttccgc gaacgcggcc aacgttctgg cgagtaccgg ctcccctgca
     2581 agatcataga aaagtttgga atcccggcgc cggcttttgc tgaaagcggc accggaactt
     2641 tgatgaaaac gagtcccaag acccgccgcg gggatgatca gactgatcgt tggaacatgg
     2701 taacctttgt gtttatggcg catgagatat caaactggaa gagttcaacg cgtcaacact
     2761 catccaaaat cgtcgcgcgt cggacgaatc ataaacgctc acaacgtctc ttttaatgat
     2821 ttgggggtgt cgcaggtcct tgcccctggt taacgctctg tccacccggc tggtgatgat
     2881 gtccattgcg acccccgttg cgctcatgac ggtcacctcg tccacgttcc cggttctcat
     2941 gccaacgatc cccgcgtccg cgatcatgac gatcccggcg cccctcatcc tcgtccgaaa
     3001 gcttcccgaa aatcatccgg ccagcctgag tctggagcac actcgtgatc gtcactttcg
     3061 ttgtctgtcc gatacgccgt cgggcggcat ccacaaccac catcgtgccg tcatcaagat
     3121 aggcaacccc ttgctcatgc tccttgcctt ccttgagaat cttgatctcc atttcctcac
     3181 cgggcatcac aacgggcttg agggcgttcg caagatcatt gatattaagt accttcacac
     3241 cctgaagctc cgccaccttg ttgagattat aatcattggt aaataccttg gcctcgatca
     3301 cctgggccat tttcaccagc tttgcgtcca ccgtattgaa ttccggaaaa tccatctcat
     3361 gaatcttgac cgtcacgttc gggttttcac ggatagcgtt catcacatcc aagccgcggc
     3421 gaccgcgatt ccttttaagg ggatcggaag aatcggaaat aagctgtagc tccttaagca
     3481 cgaaccgggg caaaatcagc tttcctccga taaagtgagt gttcgcaata tccgcgatcc
     3541 ggccgtcaat aatcacggac gtatcaagca aaatcagatc ttcttttgcg cctttagtat
     3601 ccagtcgcac aaatggaatt atcaaactaa actcatcctg tccgcgaaga atggtaataa
     3661 ttccgaggta ggaaaaaaga acggcggtca aaatcgcaaa agtgcgctcc gcctcgggag
     3721 caaaattgcc atacgccacg accatcataa aaagcttatg tgtcagaaga ccgatcgcga
     3781 ggcccaggat aatcgaaaga atattcttga ccgtatactg ctcaaaaaaa atgtctagca
     3841 aaacaaaaac ccctccgccc atcaggcaga tcgcggccga gagccagtta cgatccaaaa
     3901 aattaagccc atccagccag gaaaacgggg ccaggtaacc aaacgtcgcg caacccgcga
     3961 gaaacaacaa ccggataaaa attatcatga tcactccttt gatatttcaa aagcagaaat
     4021 attttttatg atttaaaaat gagtcgctcc cgtcacgcca cggtacgcga ctcccttccc
     4081 gtgcgtccgc cgtcccactt ccaaaaagca ctctccaggg acaggggcaa ctcacggtta
     4141 ttttaaaaac aggtaatacc attttctgga attacctgag tctttcttga gcagtttcca
     4201 gggatgcgcc cggatatcac tgacgaaggc acgcatctca ttgtaaatct catcctccat
     4261 cagcaacttc cccaaggtcc cttcgccact ttcaacctgg cccaggatgc gttcaaggga
     4321 agccgtcgac gcttcaatgt ttttgacgct ttggctcagt tctttttcag atccggaaac
     4381 cgaggtatta agcgccacgc tcaattcgga gaaattcttc accatcgtct tgaggtcggc
     4441 aatcattcct tcatcctcga cattggcgcg aaacttcttc atcatttccg aaatctcgtc
     4501 ggaaatctcg tgcgcacgat caatgagagc ttccaccggc accggactga ttccctcaac
     4561 catgtcgtca ccctttaaaa gccgggcgtc accagccacc ggggaaatct cgatatgagg
     4621 ctcactcaag acgtgcgtcc cctgaatctt aaaagtgtaa ttttccctga tttctattcc
     4681 cttttcaata aaaagcttca cctcaacaca atttttcttc ttcgtttcgt caaaataaag
     4741 ccgcacctga cttacctccc caacgcggac acccgccatc cggaccggcg cacctttgtt
     4801 gagaatgctg acatactcat aacgcacctt cacatgatag cccgagcgaa acaggtagac
     4861 cccactgaca aaaaacaggg tcagcgtgag caggataaat ccgatgatga caaaaattcc
     4921 aaccgtcatt tcgttagctg attttttcat aaaatctccg tttctttttt cctcccctga
     4981 gtgatcgggc cagtggcgga tcccgttaca aactgttgaa cgatcggatc tttggaattc
     5041 cgaatctggt gcggagtccc ggtggcaata atttttcctt cgtaaagcat ggcgatccgg
     5101 tcggcaacct tgaacgcgct ggtcatgtcg tgagtgacca cgatggatgt cacgtgaagc
     5161 cgtctctgct tctgcaaaat aagttcattg attacgtccg cgttgattgg atccagaccc
     5221 gtggtcggct catcataaag aatgatgctc ggttcatgac agatagcgcg ggcaagcccc
     5281 accctttttt tcatgcctcc cgaaagactg gacggcatca agtcctcaat attcgaaagt
     5341 cccacccaac tcaatttctc ccgaattttc atttcaatcg cttcctcaga aaaccgcgta
     5401 tgttcataaa gtgagaaccc gacattttcc cggacggtca acgaatcaaa gagagccgcc
     5461 ccctgaaaca gcatccccaa tttaagacgg aaccggtcct gctcatcggg ggtcatcgaa
     5521 aacatggagc gcccgtcaat cttgatgtca ccttcgtccg gctcaagaag agccataata
     5581 tgcttcagaa gcacgctctt tccacacccc gagcggccaa taatcacaag ggtctcccca
     5641 tcccgaacag taagatccac gccccgaagg acatccttgt ccccaaactg cttatgaata
     5701 ttccggacct cgatcatcaa cttatccctt taaaaaaagt aaaagatagc ggtgaaaagg
     5761 gcgtccgaag cgatcaccaa aataagcgac acaacaaccg cgagggtggt cgcccgtccc
     5821 actccttccg cgccatcccg agccttgaac ccgaaataac accccaccac cgtgataatt
     5881 ccgccaaaaa agaaagcctt gatcagtccc gtaataatat ctttgaccac cagggcatca
     5941 aacccccgct tgatatacat gtgggaactc atccccagtt tgaaaacagc gatgagatat
     6001 cctcccagca tcccgataca gtcggcataa atcgtcaaca taaaaagcat gcaaaccccc
     6061 gcgataaacc ggggaatgac aagatactga atgggattgg tcgcgagggc cttaagggcg
     6121 tcgatttgtt ccgtgacttt catcgtcccg agttccgcgg caatggaggc tcccacccgg
     6181 cccgccacca ccatcgccgt caacacaggg ccgagttccc ggcaaagcga caccgaaatc
     6241 aggtcggaag taaattgttc cgcggcaaac aggcgcaatt gataagcgga ttgtaacgcc
     6301 aacaccattc ccgtaaaaag agccgtcaga aaaacaagcg gtagcgatgt cacgccgatt
     6361 ttaagcatct ggtcccatac catgggccat cgaatgctcc gcctgaaaat caggacgcat
     6421 gtatcacgaa aaagaatggc aatgcctccg gcgtggtaaa aaaaattcag gcaaccagcc
     6481 ccgaaatttt caagccataa ccgcacggac accagtgttt tcaggtaact ccaacgtgac
     6541 ataaaactcc tagaatttta aactcgttcc aaccgtaaaa cccttttcat ccggacggct
     6601 ttcctcaggt tcccctgtct tggattcgaa ttgcttcacg ggccccactt caatcgacga
     6661 cacgggcttc aaatgatcaa ggtcagaacc ggccaactgg acattggctt cctgctcgtg
     6721 gagggtcact tcaagcccct tccagaccac cagccgatcc aaagtctcta tttttacgcc
     6781 tgccataaca ttgtcaagcg tcaaatcgac cgcgcccgtt aaaaagaaaa cactgcggcc
     6841 cttccagatt ttagactctt gcaggggaaa ataaggagca aagccccgga actgaataat
     6901 tccgcgatcg taatccagct ttccccattt cccgtccgag acgttgaaga caccaataat
     6961 ttcaggacgt ttcaggtcgc cttcaataag aagtttccct cccaacttac cgcccatttc
     7021 tttcgggagc ggcttagaag caaaaaattg gacctgcccg agatcgaaat ccgcggttct
     7081 aacaattaac ttcatctgtg gcgattttag ggggaaaacc atttgtcccg tcatttcaaa
     7141 cttccgtccc caggtacaac gaatcccgga gattccgtac ggcgtcacct caaaggcccc
     7201 tttcaggtca tggaaaggct cctgatttaa aataaaatag tccgtctcaa aatccgcctt
     7261 gaatcgaaat tgccgatcct gccaattcgg agaaatagca tggatataaa tccgtccttg
     7321 cgtcacgatg tcaagcccca gaaaatccag atgatccagg cggccctgca ccgcgaaatc
     7381 aagacgacaa aggttggagt ggatcgaaat acgtttcatt tcccgctcca cggcaatttc
     7441 atagtttccg gtcacaaaat caagttcaca ctttccctga taatgatcat tcaaggtcaa
     7501 gggatccaaa atccaccgat ttccttcgga gcgaatcttg ccgttaaagg aaaactttcc
     7561 cgcgtcggaa gcgtcaagaa atccttccat gaactccttg ctcatgtcaa ttttgaaacc
     7621 ggcttccatc catggtcttt ccttccccac aaccaaacgc accgttgtct caggacgccc
     7681 gtcccgattc acgaaagccc ccgatacttt cgcgttccat ccatggaggg tcgtctgcac
     7741 ctgctcaaaa gaaaggtttt ttccactcca atgcaatttt ccggtcaaac cccggagcgg
     7801 gatggggatc tcggaagaaa aatccatgcc tttcaaatcc agccaaagat catgcgtttt
     7861 ctgaaaagca tccactgtcc catgaacgtc aacccctccc acaagaattc cgcttgccgt
     7921 tgcccgaata ttgacccgtg cttttgtacc ttcttctgcc gtaatggtcc cttcgatatt
     7981 ccgaagcacg atatccttgc cgagcgatga cacccggtaa cggatttctc cgcccttgaa
     8041 atcaatcgtg ggcattccaa atcctccggg ggcgctcgcg gcagtttcaa aatactggta
     8101 gggaaatttg tcttcttcga tcatcagtga gggattccgg aacgtcactc cggcaggagc
     8161 atcgaatttg cccttccaga gatggcttaa atgataacgg aaaagcaatc ggtccagatg
     8221 tatgagttgc gagttgcccc ggatctcagg caattcgata atcacatttt caagggcgag
     8281 ttgattgaaa tgatagggcc ggatttgccc gattgagact ttggcgcgaa gatattcacc
     8341 cgcccgactt tcagcgaatc gcttgaactc ttccgaggtg gcaaactggt ggagaaacac
     8401 tttcgcggcc aaaaccaccg cgatcagaaa aaaaagcagg agcagtatga gacccttcag
     8461 aaacgaatgc ttcttcacgg atcgcttacg tcgctttctc gaaaacaacc ctgtcgccgc
     8521 ggatttcagc ctggatcttg tccccctcct ggaattttcc gctaagaatt tcttccgcta
     8581 gcacgttttc cacgtatcgc tggatcgtcc ttttgagcgg ccgcgcccca aacacggggt
     8641 catagccctg atcaatcaaa aatttgagga cttcgtcaga aagcagaagc tccatcgcct
     8701 tttccttgag acgatccttc acttcaccaa gttcgatatg aatgatctgt tcaagatcct
     8761 ggcgagacaa cggatggaaa acgatgatat cgtcgaccct gtttaaaaat tccggacgaa
     8821 acactttttt cgtctcatcc aacagtttgg tctttaacga atcgtagttc atgtcatctg
     8881 ttcttactcc gaaaccaaga tctccctgtt tccgcacaag atccgccccg acattggacg
     8941 tcatgatcac gatcgtgttc cggaaatcaa ccttgcgccc cagggaatct gtcaggcgac
     9001 cttcttccat caactgcagg aggatgttaa acatgtcagg atgcgctttt tcgatttcgt
     9061 cgagcaagac aaccgaataa ggccggcgac ggactttttc ggtcaactgg ccaccctctt
     9121 catagcccac gtatccgggg ggcgctccga ccaaccttga aacgttaaac ttctcgctat
     9181 attccgagca atccaccgtg atcacggcat cccggtcccc gaacataaac tccgccaggg
     9241 cgcgtgccag caaagtttta ccgacacccg tcggccccag aaaaataaaa gtcccgatgg
     9301 gacgtcgcgg attacgaaga cccgaccgtg aacgtctgac ggcgttagca atcgcggtaa
     9361 tcgcctcatc ctgaccgatc accccctgat ggatctcttc ctccatttta agaagccgcg
     9421 ccgtctcttt ctcttcaagg cgcgcaagcg gaacgccggt ccacttggaa accacaaacg
     9481 caatgtcctc ggctgttacc tggatttcaa cttcagactt ggattccttc cagtttttct
     9541 tcatcttctg gagttcttcc ttcgccttac gttcttcatc gcgaagtttc gccgcttttt
     9601 caaaatcctg tcccttgatg gacgcctctt tttcacggcg gagcgattca atctccgtct
     9661 ccatcttctt caaatctttc ggcaacgtcg tcaccgacaa acgggcacgg gaagccgctt
     9721 catcaataat atcaatggct ttatccggca aaaatcggcc cgtgatgtac cgatccgaca
     9781 acttggccgc ttcggagagc gcttcatccg taatcttgac gcggtgatga gcctcgtatt
     9841 tatcacgtag ccctttcagg atctgaaccg tgtcatcaac cgacggcgga tccaccatca
     9901 ccgtttggaa tcgccgcgcg agcgccgcgt ctttttcaat atatttccgg tattcatcca
     9961 gtgtcgtggc tccgatacac tgaatttctc cgcgagataa agacggcttc aggatatttg
    10021 acgcgtcaat cgcgccttca gctccgccgg ccccaaccaa cgtgtgcaat tcatcaatga
    10081 aaataatgac attatccgcg cgtttaatct cgtccatcac agccttaatt cgttcctcaa
    10141 actggccgcg atatttcgtt cccgccacca tcaacgcaag atcaagaacg atcatgcgct
    10201 tgtcgagtaa aatttcggga atatcgcccg cgatgatccg ctgggccagc ccctcgacaa
    10261 tcgccgtttt ccccactccg gcttcaccca ataggaccgg attgtttttg gttcgacgcg
    10321 cgagaatctg aatcacacgt tcaatctcgt cattccgccc cacgaccgga tccagcttcc
    10381 cttcgcgcgc gagctgtgaa aggtcacggc caaaagcgtc cagcgccggt gtttttgttt
    10441 tcgacttact tcctccacta cccattccgg aagcttgcgc ccccccgctc attgccgctg
    10501 aatcgctccc cggcgtggct gaaccaagaa gtttgatcac ctcggcgcga accttgttca
    10561 ggtcaagacc gatattcatg aggacatgac tcgcgacacc ttccccttcc ttaatcagtc
    10621 ccagcaaaag gtgctccgtc ccgatatagt tatgccccag acgccgcgct tcatccagcg
    10681 cgagctcaat aacttttttc gctttcggcg taaaaggaat gtcgcccgaa acgaccgtac
    10741 tcggcccaaa ctggacaagt ttttcgatct caagccggat cgtgtcaagc gaaagtccga
    10801 ggttctgcaa cacagccgcg gcgacgcttt ccccctcctt gatcagcccg agcaaaatat
    10861 gttcggttcc gatgtaatca tggttaaatc gtttcgcctc ttctttcgcg agaataataa
    10921 cttttctggc tctttccgta aaacgatcaa acatttcaac ctccagataa tcgatctttt
    10981 tattaaaaaa atacgcggcc gggcaaacgc cgctcgtaat ttttgactct ctattcgaga
    11041 ccctcgactg tcatatcccg agggaattcg accacaaccg tgtaaaaaat ttcgaccttc
    11101 cccccggcgg caagttgcaa ctgccaacgc cagacaccct tcctgtcctt ccaatccttg
    11161 tcttggggat cttggctgac ctgggaaact ttgaccttga tgcgctcatc ctgcgaaacc
    11221 ggaatggtct cgaacagatt aagcttgccc gcctgggcct tataattttc ggcagttagc
    11281 ttatatttat aagtcacttt cctgttgggc gacggaatat tggcgatcag cacatcgtcc
    11341 gatttctttt cgagcaactc acgttttact ttcacgccct cgtccacgcc aaggtaaagg
    11401 tcaaactctt ctcccggagc gatattctgg acataagaat ttcccacaaa atctccgtca
    11461 agaaagatat tcaccggccc gctcaagagt tgaaggtctt ttgcgtttgt cacccggctc
    11521 gtgagatagg cgagtggcac cattctggga tacgccgaat actcgaaaga agaagaaaga
    11581 atttgcgcgg taaccgggac cttttgatca gaaccatccg gacgaaccgt cacttttcgg
    11641 gtcagcttgt aaacaacgga tactcccttt tgggcagctt gcgcgtagag gttctcagct
    11701 tctaaagcca tattggttgc tagcataggt tcctccgctt ctccccccga tctcatggcc
    11761 atatcggcca tcccaagact tttggctcgc aatttcatgg acgcacccct tagaggctgg
    11821 cgcggctgga aaggatggac aaaccagctg ttgacttcag gcatcgtccc accaacgctc
    11881 acctgagccg tcgagagtgt catatccaca tcctcccaca cttccccggt gccctgactc
    11941 acgatcgcgt aagtgacaag ctcggtctca gatttatcaa actgcgcgcg cgcatcatag
    12001 acgggcgacc acgaggcccc gctcaccttg taggaaacct cgagatccag atgaccggca
    12061 cgggacactt ccacgtcaac cgtcacgatc tggcgcgtct tctgccgccc tcctgaaatt
    12121 tcacccaatt cgcgtcgcaa ggcgaccagc ttttttccgt tttctcggat ctcaagatca
    12181 atcgcgtgaa cacggctgaa attttctttc agtttcgatc caagaaaact gagggtgcct
    12241 tccaggtcct gcggggacgg cattttcgtc acaagatctt cagggatttg ctggcccgcg
    12301 aacaaacgaa ccgaatcaag atacgccttc tcctccgtca ggatttgttt ctcgttctgc
    12361 caggcgcgat tttgatcttc aagcgtctga atcctgtttt ccagttcacg cactttctca
    12421 accgaggttt cctgtaaaaa ctcttttttg acctgc
//
LOCUS       DAOSIO010000024        12437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_5106_length_12437_cov_4.277419, whole genome shotgun sequence.
ACCESSION   DAOSIO010000024 DAOSIO010000000
VERSION     DAOSIO010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 12437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12437
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5106_length_12437_cov_4.277419"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /gene="rpsO"
                     /locus_tag="PK590_01775"
     CDS             <1..181
                     /gene="rpsO"
                     /locus_tag="PK590_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOG23358.1"
                     /translation="ILTERINGLSPHFEKHAKDHHSRYGLIKMVSRRKKLLAYLKRED
                     PEKYQSLIASLELRK"
     gene            238..2418
                     /gene="pnp"
                     /locus_tag="PK590_01780"
     CDS             238..2418
                     /gene="pnp"
                     /locus_tag="PK590_01780"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOG23359.1"
                     /translation="MFKIERVSSNIGGKEIIIETGHLARQAHGAVTVQCGGTVVFVAA
                     VAGGDTDQVRDFFPLTVDYRERTYAAGKIPGGYFKRESKPTEKETLTSRLIDRPLRPL
                     FPEDYLTEVQISAIVLSFDGENPTEVLSMIGASTALLISDIPYTVPLSAVRVGLVDGN
                     FIINPSQRELEKGDLDLVIAGTPEKVVMIEAGAREISEEKMFEAIKFGHEAIKKTAAL
                     QMELVKKAGKPKKAVKPVEIPAEITQKVNEVAANEFQKVFGLPTKEEQEGFFKSLYEK
                     IMAQLDTKAEGFDETFVKIAFSRLEHEKVREHMLATGKRADGRGQRDIRPIKCETGIL
                     PRTHGSSLFTRGQTQSLSVVTLGTGDDEQRIDALEGEQLRRFMLHYNFPSYSVGECKP
                     NRGPGRREIGHGALAARSLEPVIPFDENFPYTVRLVSEIMESNGSSSMATVCGGTLAL
                     MDAGVPIKAPVAGIAIGLVTDKSRWKVLTDIAGIEDHLGDMDFKAAGTRDGLTALQMD
                     LKIDGITDEILHEAFDHAKQARFKILDMIAEAIHAPKELSSYAPRITTLRINPEKIGA
                     LIGPGGKNIRKIVEETGAKIDIEDDGRVFVAAVDAAVSDAAIARIKGCTAEAEVGKIY
                     EATVQKLMPFGAFCEILPDLDGLCHVSEIADGFVKSPGDYMRVGDKVTVKVLGVDERG
                     KISLSIKAAVPGGLPILPEDQRVVVEAEDRGPRRGGGRGGRDRR"
     gene            2482..2931
                     /gene="dut"
                     /locus_tag="PK590_01785"
     CDS             2482..2931
                     /gene="dut"
                     /locus_tag="PK590_01785"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOG23360.1"
                     /translation="MNKLKVRVKTFSHFDGLELPRYMTEGSSGIDLLAACADNIELKP
                     GERKLVPTGLAISLPRDTEVQIRPRSGLAIKHGITILNTPGTIDSDYRGEIQIILINL
                     GQELFTIKRGMRIAQMVFGQVVKAELQMADNLDETCRGAGGFGHTGH"
     gene            3017..5395
                     /locus_tag="PK590_01790"
     CDS             3017..5395
                     /locus_tag="PK590_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOG23361.1"
                     /translation="MKKERINEIWGVFFLLLGLFTLGALLSYHPEDISFYTSHPSEPA
                     RNWTGLIGAYLAFGLFLAFGQSAFVIPLVLLLWARSFFYQQVPERKMFKFLGLGIALF
                     SLATIVSITVNPEMSLTAGGAAGYVVGKWLHGSLGKLGSYIFAGAALLLSVLLATDFL
                     IFPLVKKIAENLRAVFARKPQKPVKAQKESRGFFSAMKEKKAAKEKQKEKAEIKLPPE
                     FANPTKIKAYQPISPDPNKKTEGGRPEFGKEDSRKPLPSPTVKLARNGKDSSKNETSA
                     DDADNSFKGIIGSDATVQRYEFPPTDLLRLPKTEKIEGDDLAANSRAIEGTLKEFGID
                     VKVVEVEQGPKITLYELLPAPGVRANSISAYEDDLSMALKATSIRLIIPIPGKQTIGV
                     EVPNSVSRPIVIREMIEHPDFRSHRQRLPLLLGKDTAGKPMIADLTTMPHILIAGTTG
                     SGKTVCIKSIIAGFLYYCTPAQLKMVMIDPKMVELAKYNKIAHMLSPVVTDVRKAANT
                     LNWVVQEMERRYRLLAAVGVSNIQSFNERELTEEDKKQVTEAAKDSENVIPASLPYIV
                     VIIDELADLMLVAQDKVETAITRLAQLSRAVGIHLILATQRPSVDVITGVIKANFPAR
                     VAFKVASVVDSRTILDNKGADKLLGKGDMLFIQPGSDNMIRGQAPLIMDDETDRIVKF
                     VAQQGQPEYHSEIQAVQNGTANAATGGEKDELFDEAKRIVLTSRTASTSYLQRRLGLG
                     YTRAARIIDQLEHAGVIGPQQGAKPREILMPNPAGDYSEATPVSSEGSEENV"
     gene            5439..6017
                     /locus_tag="PK590_01795"
     CDS             5439..6017
                     /locus_tag="PK590_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013934308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOG23362.1"
                     /translation="MTGKRVLLHACCAPCCASIIKRLSDSGFEVTVFFYNPNVYPREE
                     YAKRKQEVIRYAGKNRIFFVDGDYDPDRWFEWTKGYDESPERGERCGLCFDMRLKKTA
                     AYAAENGFQVFTSSLGISRWKDFDQVTRSGKKAASLFPGTIYCDINWRKDGGIELMSR
                     ITHEEKFYRQDYCGCLYSLGNYKQKTKNFPAS"
     gene            complement(6109..6486)
                     /gene="crcB"
                     /locus_tag="PK590_01800"
     CDS             complement(6109..6486)
                     /gene="crcB"
                     /locus_tag="PK590_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOG23363.1"
                     /translation="MAKWIALLSGGALGTLARYGLGGIIHQFLGSSFPYGTLAVNLTG
                     CGLIGFLAVLSEDKFLLSPSSQIFLMIGFCGAYTTFSAFMLETANLMKDGENVRAFMN
                     IFASIVFGFLALKTGILIAKLIN"
     gene            complement(6491..7054)
                     /locus_tag="PK590_01805"
     CDS             complement(6491..7054)
                     /locus_tag="PK590_01805"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOG23364.1"
                     /translation="MKKSLGKKTILFPSPILIVCSYAADGTPNAMNVAWGGIACSEPP
                     AVAISVRETRKTFENIMTQKAFTVCIPSAKYIKEADYFGVVSGKNENKFKTTGLTPVK
                     ADHVNAPYIKEFPLALECRLLQTVKLGSHTQIIGEIVDVKADETILNAKGFPDIEKMQ
                     PCVFDPGTSSYYGSAKFLTQAFQAGKK"
     gene            complement(7063..7392)
                     /locus_tag="PK590_01810"
     CDS             complement(7063..7392)
                     /locus_tag="PK590_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOG23365.1"
                     /translation="MRAIVLLTISNIFMTIAWYGHLRFKHAALWKVILISWLIAFVEY
                     CFQVPANRIGSYQFETAQLKTIQEIITLIVFCVFSVVYLKEGLKWNYLVGFAFIVLAA
                     FFIFKKW"
     gene            complement(7401..7880)
                     /locus_tag="PK590_01815"
     CDS             complement(7401..7880)
                     /locus_tag="PK590_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HOG23366.1"
                     /translation="MKTLSILKLGGSVLFCMSAGAVSSLYTLKAIPDWYATLNKPSFT
                     PPSWTFGPVWSVLYVMMGIAAWQVWSKGLKERAVRKALVFFLIQLVLNTAWSFLFFGL
                     RSPILGLVDILLLWLAILITINLFFKVSLIAAVLLIPYLFWVTFASGLNLGIVLLNP"
     gene            complement(7877..9466)
                     /locus_tag="PK590_01820"
     CDS             complement(7877..9466)
                     /locus_tag="PK590_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG23367.1"
                     /translation="MKIVLLQPPIRDFYETDIRLQPLGLCYLKATIQKHFSSVRVIVK
                     DYHAGWGRRTLPWPRELTYLKPYYGKADKSPFCTFHHFYHFGADYETIARDLKKESPD
                     LIGISSLFTAYYQEVLETASAIKREIQVPIFAGGSHASALPETLLAHPAIDFVIRGEG
                     ERAIVKFLKQWQRKADSLSSKKKLVPSPTRNFPVDKIPFPDFSDLSRSHYLYEGKPLC
                     FILTSRGCPYHCSFCSVHETFGHQYRRRSNDNILREIQLRYEQGYRVFDLEDDNLTFD
                     KKAACLLFKKIILLFPKNDLQFLAMNGICYWHLDHELLSLMRQAGFTHLNLSLVSSNP
                     SVCQTARRPLDLKKYRSVIEDAFRLGFKIVSYQILGLPGETEHSMARTLILHAKLPVL
                     IGASPFYVAPGKVNEPPPSTVNTRLTGLLAWTTPKQRDSVYTFFVITRILNFLKGISL
                     QGDSSLKTALRVARKSERRSVAGVKILKMLLEGRGLHGWNHDSFELQPRFRTEHFYKV
                     WRKIDRIATQEGRSIILKGKL"
     gene            complement(9482..10030)
                     /locus_tag="PK590_01825"
     CDS             complement(9482..10030)
                     /locus_tag="PK590_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23368.1"
                     /translation="MTWEKPWKDSEVSYSLKREDGHYVLKATLRVNSDPGCLIQVVFS
                     SYHMQKLMAKDADRIEILHETPGYYELRSVHHNKLCRLDTVYSRRLSETDGRIAFELI
                     RCTQESKVAPKLVSSKGYYEIKPDSKGAILSYYEKTRLEAKRWDPRAMALVKIVARDT
                     VLFLKKIKKYSETSACRHAPKK"
     gene            complement(10105..10815)
                     /locus_tag="PK590_01830"
     CDS             complement(10105..10815)
                     /locus_tag="PK590_01830"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOG23369.1"
                     /translation="MNPYTRHPILYEDNAMVVIHKPSGVLSHPNVKGQPAAYEGPYDP
                     DRRIFQTPAGPLWLIHRLDQETSGILLAAKTPESAKIFRTSFEKKEVEKDYVVLVARK
                     PLPPQGRWSDFLAEKKRAGGIKVFVTKGPKPNALLHYITKDFYPRTNLALIEVRLVTG
                     KTHQIRVQAAYHGHPVAGDRVYGNFAVNKELRHHLGLNRLFLHAWRIKIRHPSSSKIL
                     DIETALPEELEKALGRAQ"
     gene            complement(11168..11440)
                     /locus_tag="PK590_01835"
     CDS             complement(11168..11440)
                     /locus_tag="PK590_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOG23370.1"
                     /translation="MDLKVFTATLSAIFFAEIADKTQLVSLTMAGKTAKPWAVLLGAV
                     AGFSLVTIFSIVAGTLLGKYLHPEHIRYVGASAFVVIGILMFFNKI"
     gene            complement(11842..>12437)
                     /locus_tag="PK590_01840"
     CDS             complement(11842..>12437)
                     /locus_tag="PK590_01840"
                     /inference="COORDINATES: protein motif:HMM:NF037962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HOG23371.1"
                     /translation="EAIPVILAQKDQAQLVLDILGVKIIIATVAGYLIDAFHRPDTHA
                     PHAPVPRVGEHLHCSCHSHSHNEPWWKTYLLFPLKHTASISVFIFLISIALGFLIETA
                     GEENLGKIFFHGSLFQPALAVAIGLIPNCAASVAIAEMHLKGVITFGSAIAGLCASGG
                     LGILVLIKESKDMKETLAILSLLALISLAAGIVLNLF"
BASE COUNT         3200 a         2839 c         3322 g         3076 t
ORIGIN      
        1 cattctaacc gagcgtatca acgggctttc ccctcatttt gaaaagcacg ccaaggacca
       61 tcattctcgc tacgggctca tcaagatggt gagtcgccgc aaaaagttgc ttgcctatct
      121 caagcgtgaa gacccggaaa aatatcagag tctcatcgcg agtctcgaac tcaggaaata
      181 atttcctgtt tttccgcacg tcccagaatc tattcaaaat tgaaaaggaa cccacccatg
      241 tttaaaatcg aaagagtcag ttctaatatc ggcggtaaag aaattatcat agaaacagga
      301 catttggccc gtcaggccca cggcgccgtg accgtccaat gcggcggaac cgtggtcttt
      361 gtggcggcgg tcgccggagg cgatacggat caggtacgcg atttttttcc tctgacggtg
      421 gattatcgcg aaagaactta cgcggccgga aaaatccccg ggggctattt taaaagggaa
      481 agcaaaccca ctgaaaaaga aacgctaact tcccgtctta ttgatcgtcc cttacggcct
      541 cttttcccgg aagattatct gacggaagtg cagatcagcg cgatcgttct ttcgtttgac
      601 ggagaaaatc ccacggaagt tctttcaatg attggggcat cgacagcgct ccttatttct
      661 gacattccct atacggtgcc gctttccgcg gttcgtgttg gattggttga cggaaatttt
      721 attatcaatc cttcccagcg ggaacttgag aagggggatc tggatcttgt gattgccgga
      781 actccggaga aagttgtcat gattgaagcg ggggccaggg agatctctga agaaaagatg
      841 ttcgaggcga tcaaattcgg gcatgaggct atcaaaaaaa cggcggccct tcagatggag
      901 cttgtaaaga aggccggtaa acccaaaaaa gccgtgaaac cggtcgagat tcccgccgaa
      961 atcacgcaaa aagtaaatga agtcgcggct aacgaatttc aaaaagtgtt tggtttgccc
     1021 accaaggaag agcaggaagg ttttttcaaa agcctttatg aaaaaatcat ggcccagctt
     1081 gacaccaagg cggaagggtt cgacgaaacg ttcgttaaaa tcgctttttc tcggctggaa
     1141 catgaaaaag ttcgtgagca catgcttgcc accggcaaac gcgcggatgg gcgaggacaa
     1201 cgggatattc gtccgatcaa gtgtgaaacg ggaattcttc cgagaacaca cgggtcgtcg
     1261 ctttttaccc gcggccaaac acaaagtctc agcgtggtca ctttggggac gggggatgat
     1321 gagcagagga tcgacgcgct cgaaggtgaa cagttgaggc gatttatgtt gcattacaat
     1381 tttccttcct acagcgtcgg agagtgtaag cccaaccgtg gtcccggacg tcgtgaaata
     1441 gggcacggcg ctcttgccgc gcgttctctt gaacccgtga tcccgtttga tgaaaatttc
     1501 ccgtacacgg ttcgccttgt ttccgagatt atggaatcga acggatcaag ctccatggcg
     1561 actgtttgcg gtggaacact cgccttgatg gacgccggtg tgccgataaa ggcgcctgtc
     1621 gcgggtatcg cgatcggtct tgtgacggac aagagccgct ggaaagttct gacggatatt
     1681 gccggtatcg aggatcatct gggggatatg gattttaagg ccgctggaac ccgggacggg
     1741 ctgaccgcgc ttcagatgga tcttaaaatt gacgggatta cagatgaaat tctccacgag
     1801 gcttttgatc acgcgaaaca ggcgcggttc aagattttgg acatgatcgc ggaggcaatt
     1861 cacgcgccca aagagctttc ctcctacgcg cccaggatca cgacgcttcg cattaacccc
     1921 gagaagattg gagcgctgat tggtcccggg ggcaagaata ttcgcaaaat cgtggaagag
     1981 acgggcgcca agatcgacat cgaggatgac gggcgggttt ttgtcgcggc cgtcgacgcc
     2041 gcggtttcgg atgccgcgat cgccaggatt aaaggatgca cggctgaagc tgaggtaggg
     2101 aaaatttacg aagcgactgt ccagaaactg atgccctttg gcgcgttttg cgaaattcta
     2161 ccggaccttg acggtctttg ccacgtttcg gaaattgccg acggttttgt caaaagcccc
     2221 ggggattata tgcgggtggg cgacaaagtg acggtcaagg tgctaggggt tgatgaacgc
     2281 ggaaaaatct ctcttagtat caaggcggcg gtgccgggcg gacttccgat ccttcccgag
     2341 gatcagcggg tggtggtgga agccgaggac agggggcccc gaagaggggg tggacgcggc
     2401 ggccgggatc gcagataact tcaaccggat ttcagaaagg gtagcctccg cgaactgaac
     2461 ggaagctgcc ctttttttct tatgaacaaa cttaaagtgc gagtgaagac gttttctcat
     2521 tttgacgggt tggagcttcc gcgatatatg acggaaggtt caagcggcat tgatctgctt
     2581 gcggcttgcg cggataacat tgagttgaaa cccggagagc gaaaacttgt tcccacgggc
     2641 cttgcgatta gtttgcctcg cgacacggaa gtccagatcc gtcccagaag cgggcttgcg
     2701 atcaagcacg gaatcacgat cttgaataca cccggaacca ttgattccga ctatcgcggg
     2761 gaaatccaga ttattttgat caatttgggg caggagttgt ttaccattaa acgaggtatg
     2821 cgtattgccc agatggtttt tggccaagtg gtaaaggctg agctccagat ggccgataac
     2881 ctcgatgaga cttgccgggg cgccggagga tttgggcata ccggtcatta atcatttacg
     2941 gcggtcggca tttaccgatg ccatcatcgc ccttcctgtt tgctgtgtgc cgaccgctaa
     3001 acactgattt gaatttatga aaaaagaacg tattaatgaa atttggggtg ttttttttct
     3061 tctgctgggc ctgtttacgc tgggagcttt gctgtcttac catcctgaag atatttcgtt
     3121 ttacacctct catccttctg agcccgcccg gaactggacg ggattgatcg gggcttatct
     3181 ggcttttgga ctttttttgg ctttcggcca gagcgctttt gtgataccgc ttgtccttct
     3241 gctttgggcg cgcagttttt tttatcagca ggtcccggaa cgtaaaatgt ttaaatttct
     3301 cggtcttggg atcgctcttt tttcgctggc gacgatcgtt tccattacgg ttaatccgga
     3361 aatgagtttg acggccggtg gcgccgcggg atatgttgtc ggaaaatggc tccacggcag
     3421 tttgggaaag cttggcagtt atattttcgc gggcgcggcg ctccttctgt cagtgttgct
     3481 tgcgactgat tttcttattt ttccgctcgt aaaaaaaata gcggagaacc ttcgggcggt
     3541 cttcgcgcgg aagcctcaaa aacccgtcaa agctcaaaaa gaatcccgtg ggtttttctc
     3601 cgcgatgaaa gaaaagaagg ctgccaaaga aaaacagaaa gaaaaagctg aaattaaact
     3661 gcctccggaa ttcgcgaatc ccaccaagat aaaagcctac cagccgattt cacccgatcc
     3721 caataaaaaa acagagggcg gtcggccgga atttggcaaa gaagattctc gcaaacccct
     3781 tccgtccccg acggtgaagt tggcacggaa cggaaaagat tcctccaaga acgagacatc
     3841 ggccgatgac gcggacaatt cgtttaaggg gatcatcggg tccgacgcga ctgtccagcg
     3901 gtacgaattt cctcctacgg atcttctcag gctcccgaaa acggaaaaaa tagaagggga
     3961 tgacctagcc gcgaattccc gggctattga gggaaccctc aaggaatttg gtattgacgt
     4021 gaaagtggtg gaggtcgagc agggccccaa aattacgctt tatgaactcc ttccggcgcc
     4081 gggtgtgcgg gcgaattcga tatccgctta tgaagacgat ttgtcgatgg cgcttaaggc
     4141 gacgagtatc cgtcttatta ttccgatccc cggcaaacaa acgatcgggg ttgaagttcc
     4201 gaactccgtg tcgcgcccca tcgtgattcg cgaaatgatc gaacaccccg attttagatc
     4261 acatcgtcag cgtctcccgc ttcttctcgg gaaagatact gccgggaagc cgatgatcgc
     4321 ggatttgacg acgatgccgc atatcctgat tgccggaacc acgggaagcg gaaaaacggt
     4381 ttgtatcaaa tcgattattg cgggtttcct ttattactgt acgcccgccc aactcaagat
     4441 ggtcatgatt gatccgaaga tggtggagct ggcgaaatat aacaagattg cccacatgct
     4501 ttcaccggtg gtgactgatg tccgtaaggc cgcgaacacc ctcaactggg ttgtccagga
     4561 gatggaacgc cgttaccggc tcttggccgc cgtgggggtc agtaacatcc agagttttaa
     4621 cgaacgtgaa ctgactgaag aagacaagaa gcaggttacg gaggccgcca aagattccga
     4681 aaacgttatt cccgcgtctt tgccctacat tgtcgttatc atcgatgagc tcgcggatct
     4741 catgctggtc gcccaggata aggtcgaaac ggccatcacg cgccttgccc agctttcccg
     4801 agcggtgggc attcacctga ttcttgcgac acagcgtcct tccgtggatg tgattaccgg
     4861 agtcattaag gctaatttcc cggcccgcgt cgccttcaag gttgcttcgg tcgtggattc
     4921 ccgaacgatc ttggataaca aaggcgccga caaactgctt ggcaagggag acatgctttt
     4981 tattcagccg ggaagtgata atatgatccg tggacaggct cccctgatta tggatgacga
     5041 aacggacaga atagtcaaat ttgtcgccca gcagggacag ccggaatatc acagtgaaat
     5101 tcaagccgtc cagaacggaa cggctaatgc cgcgacgggt ggtgaaaaag acgagctttt
     5161 tgatgaagcg aaacggattg tcctgacaag ccggaccgcc tcaacctcgt atcttcaaag
     5221 acgtctcggc ctcggctata cccgcgcggc gcgaatcatc gaccagctgg aacacgccgg
     5281 ggtgattggt ccccagcagg gggcgaaacc ccgcgaaatc ctgatgccaa atccggcagg
     5341 ggattattcc gaggccactc cggtcagttc agagggatcg gaagaaaacg tctgaggaag
     5401 ggacaccctt gatcccgatg aaaatttgtc ttcccgaaat gactggtaaa agggttttgc
     5461 ttcatgcctg ttgcgcccct tgttgtgctt ccatcataaa acgattaagt gattccggtt
     5521 ttgaagtgac cgtgtttttt tacaatccga atgtttatcc gcgtgaggaa tacgccaagc
     5581 gcaaacagga agtcatccgg tacgcgggga aaaaccggat tttttttgtc gatggagatt
     5641 atgacccgga tcgctggttt gaatggacaa aaggctacga tgaatccccc gaaagaggtg
     5701 agcggtgcgg gctttgcttc gatatgagac ttaagaagac agccgcttat gccgcggaaa
     5761 acggatttca agtttttacg tcaagcctcg ggatctcgag atggaaggat tttgaccagg
     5821 tgacccgatc agggaagaag gcggcatctc tttttcccgg aacgatctac tgcgatatta
     5881 actggcggaa ggacggcggg attgaattga tgagccggat tacccatgaa gaaaaatttt
     5941 accgccagga ctattgcggg tgtctttatt ctttgggaaa ttacaaacaa aaaaccaaaa
     6001 acttccccgc aagttaattg cggagaggtt ttccttcccg cgcgtccgct tctctgataa
     6061 aaattccggg caacttttca tctgccggaa gtttcatgga aattcccgtt aattaatcag
     6121 ttttgcgatc agaatgccgg tttttaaggc caaaaaaccg aaaactatac tcgcaaaaat
     6181 attcataaaa gcacgaacgt tttctccgtc tttcatgaga tttgcggttt cgagcataaa
     6241 ggctgaaaaa gtggtatagg cgccgcaaaa cccgatcatg agaaaaattt ggctggaggg
     6301 ggacaagaga aatttgtcct cactcaggac ggcgagaaaa ccgatcagtc cacagcccgt
     6361 caggttgacg gccagagttc cgtaggggaa agatgatccc aagaactggt ggatgattcc
     6421 tcccagtccg taacgagcga gggttccaag cgccccgcct gagagaagag cgatccattt
     6481 ggccataaag ttattttttt ccggcctgaa acgcctgggt tagaaacttc gcgcttccgt
     6541 aataggacga agtccccggg tcaaagacgc aaggttgcat cttttcaatg tctggaaatc
     6601 ctttcgcgtt aaggatcgtc tcatcggctt ttacgtccac gatctcgccg ataatttgcg
     6661 tgtgacttcc cagtttgact gtttgaagca gacggcattc gagtgcgaga ggaaactcct
     6721 tgatgtaggg cgcgttaacg tgatccgcct tgaccggcgt caaacctgtt gttttaaact
     6781 tgttctcatt ttttccggaa acgacaccaa agtaatccgc ttctttgatg tattttgcgg
     6841 acgggatgca aacggtgaaa gctttttgag tcataatgtt ttcaaaagtt tttcgagttt
     6901 cccggacaga gatcgcgaca gccggaggtt ccgaacaggc gatgccgccc caggccacgt
     6961 tcatcgcgtt cggggttcca tccgcggcgt aggagcaaac gatcaatatg ggagacggga
     7021 aaagaatggt ttttttgccg agagattttt tcatggatta cctcaccatt ttttaaagat
     7081 gaaaaatgcg gccaatacga tgaaagcaaa accgaccaga tagttccact tcagcccttc
     7141 cttcaaatac acaactgaaa aaacacaaaa aacaatcaag gtgataattt cctgaatggt
     7201 tttaagctgg gccgtctcaa actggtatga tccgatccgg ttggctggca cctggaaaca
     7261 gtattcaacg aaggcgataa gccagctgat aaggataacc ttccagagcg ccgcgtgttt
     7321 gaaccgcaaa tgcccatacc aggcgatggt cataaaaatg tttgaaattg tcaaaagaac
     7381 aatcgcgcgc ataggccgtt ttagggattg agaagaacaa tccctaaatt aagtccactc
     7441 gcgaaagtga cccagaacaa ataagggatc aggagaaccg ccgcgatcag cgagactttg
     7501 aaaaacaaat ttatcgtgat aaggatggca agccataaca gtaaaatatc gacaaggccg
     7561 aggatcgggg aacgaagtcc aaaaaacaaa aaagaccagg ccgtattcag gacaagctga
     7621 atcagaaaaa agacaagagc ttttctgacc gcccgttctt tgagtccctt ggaccagact
     7681 tgccacgccg ctattcccat catgacataa agcacgctcc agacggggcc gaaggtccag
     7741 ctcgggggcg tgaaggacgg tttattcagg gtggcatacc agtcggggat ggccttaagg
     7801 gtatagaggg aactcactgc ccccgcgctc atacaaaaca gcaccgatcc cccaagtttt
     7861 agaatcgaaa gcgttttcat aattttccct tcaggataat cgagcgtcct tcctgggtgg
     7921 caattctatc aattttgcgc cagactttgt agaaatgttc tgtcctaaat cggggttgaa
     7981 gctcaaagct gtcgtggttc catccatgga gacctcgccc ctccaataac attttcagga
     8041 tcttgacgcc tgccacggag cgtctctcgg attttcgtgc gacgcgaagc gctgttttta
     8101 aagaagaatc tccctgaagc gaaattcctt taagaaaatt taaaatgcgt gtgatcacaa
     8161 aaaacgtata aacagaatcc cgctgtttgg gggttgtcca tgccaaaagt ccagtcaggc
     8221 gcgtattgac ggtcgaggga ggcggctcat tgactttgcc tggagccaca taaaacgggg
     8281 acgcgcctat cagcacggga agttttgcgt ggagaatcag cgtgcgggcc atggagtgtt
     8341 cggtttcgcc cggcagtcca aggatttgat acgaaacgat tttgaaacca agtctgaacg
     8401 cgtcctctat gacggaacga tattttttca ggtcaagcgg ccgacgggcc gtttgacaga
     8461 cagaggggtt ggagctgacg agggaaaggt tgagatgcgt aaaacccgcc tggcgcatga
     8521 gtgagaggag ttcgtgatcg agatgccagt agcagattcc gttcatcgcg agaaattgca
     8581 gatcattttt ggggaaaagg aggattattt ttttaaaaag taaacaggcc gcttttttgt
     8641 caaaagtcag gttgtcatct tcgaggtcaa agacccgata accctgttcg tagcgaagct
     8701 gaatctcccg caaaatatta tcgtttgacc ggcgcctgta ttgatgccca aaggtttcgt
     8761 gaacggaaca gaatgaacag tgatagggac atcctcttga ggtgagaata aaacagaggg
     8821 gttttccttc atacaggtaa tgagatcggg agaggtctga aaagtcgggg aaaggaattt
     8881 tatcaaccgg gaaattacgc gtcggggaag ggactagctt cttttttgaa cttaaggagt
     8941 cagctttcct ttgccactgt ttcaaaaatt tcacgatggc ccgttctcct tcgccccgta
     9001 tgacaaaatc aatggcaggg tgggcgagca gggtttcggg gagggcggaa gcatgtgatc
     9061 cacccgcgaa aatcgggacc tggatttccc gcttgatcgc gcttgctgtc tcaagaactt
     9121 cctgatagta agccgtaaaa agggaagaaa tgccgattag atcgggggat tcctttttaa
     9181 ggtcacgggc aatcgtttca tagtcggcgc caaaatgata aaagtgatga aaagtacaaa
     9241 aagggctctt gtccgctttg ccgtaatagg gtttcagata ggttaattcc cgtggccagg
     9301 ggagggtcct tcgaccccat ccggcgtgat aatcctttac gatgacccgg acggacgaaa
     9361 aatgtttttg gatggtggcc ttgagataac acaacccgag gggttgcagg cgtatgtcgg
     9421 tttcataaaa atcgcggatg ggtggctgga gaagaacgat tttcatgagg gtcaggagac
     9481 tttatttttt cggagcgtgt cggcaagctg aggtctctga gtattttttt atttttttaa
     9541 gaaacaaaac ggtgtcacgc gccacaattt tgacaagcgc catagccctc ggatcccaac
     9601 gcttggcctc aagccgtgtt ttttcgtagt agcttaaaat ggcccctttc gaatccggct
     9661 tgatttcata ataacccttt gaactcacca gcttgggcgc gactttactt tcctgggtac
     9721 atcggatcag ctcaaatgcg attcgcccat cagtttcaga aagacggcgg ctgtaaacag
     9781 tatcaagacg gcaaagcttg ttatgatgaa cggagcggag ctcataatac cccggagttt
     9841 cgtgaaggat ttcgattcga tccgcatctt ttgccatcag tttttgcata tgataggagc
     9901 taaagacgac ttgaatcagg catcccgggt ctgaattcac ccggagtgtt gcctttagaa
     9961 cgtaatgccc gtcttcacgt ttgagtgaat atgaaacttc agaatccttc cagggttttt
    10021 cccaggtcat tcccaagtga gccgtgagga tgaggagaaa aaatataaaa atgagtggat
    10081 agacgcgaga tcgaaacagt tgaatcattg tgcccttccc aacgccttct caagctcctc
    10141 cggaagggcg gtttcaatat caagaatttt actgcttgaa ggatgcctga tttttatacg
    10201 ccaggcgtgc agaaaaagtc ggttgagccc taagtgatgt ctgagctcct tgttcacggc
    10261 aaaatttcca tagacgcggt cgccggcgac cggatggcca tgataagctg cctgcactcg
    10321 aatttgatgg gttttccccg tgactaagcg gacttcaatc aacgcgaggt ttgtccgcgg
    10381 ataaaaatcc ttcgtgatgt aatggagcaa agcattgggt ttggggccct tcgtgacaaa
    10441 aaccttaatg ccgcccgccc gttttttttc ggcaagaaaa tccgaccaac ggccttgggg
    10501 ggggaggggt tttctcgcaa caagcactac gtagtctttt tcgacttctt ttttttcaaa
    10561 agaggttcgg aaaattttcg cggactccgg agttttcgcg gccagcaaga tcccggaggt
    10621 ctcctggtca aggcggtgaa taagccagag cgggccggcc ggagtttgga aaattcgccg
    10681 gtcaggatcg taaggtccct cataagccgc gggttggcct ttgacgtttg gatgagaaag
    10741 aacgccggac ggtttatgaa tgacgaccat cgcgttgtct tcgtaaagga tggggtgtcg
    10801 tgtgtaaggg ttcatgacgc atcgcgatat gaccggcgtt cacttttccc gcggttacaa
    10861 aggcagagat ttttgaaagc ttgctctttg agctttttat ccagactttc cattggcttt
    10921 ttaagaaacc ttttgggggt tttgtttgcc ttgaggttgc agggtttaca tcgtgtataa
    10981 aacaaagtcc gacttcatct ttcgacaaag tcggactttg taaaggcggc tgattaacgg
    11041 cagcaacggc cggttttctt tgctttcttc ttggcctttt tcgctggttt ctttttcgcg
    11101 gttttttttg ctttctttgc catttcatgc tcctttgttt tagtcgggac aagtcgtccc
    11161 gggttggtta gattttgttg aaaaacatta aaattccaat aacgacaaac gcggaggctc
    11221 ccacatagcg aatgtgttcg ggatgcaggt acttcccaag cagagttccg gcaacgatgg
    11281 aaaagattgt gacgagtgaa aaccccgcaa cggccccaag cagtacggcc caaggcttgg
    11341 cagtttttcc tgccatggtc aaactgacaa gttgtgtctt gtctgcgatt tccgcgaaga
    11401 agattgccga aagagtcgcg gtaaacactt ttaaatccat gaaatcctcc gtcaagcttt
    11461 cttaacgagg tgattttatc tcaaaattgg gagaggggga agcttaataa cgagatttat
    11521 gaaaaatttt tttaaaaatc aatcattctc aaccgatttt tgaagaaaat cgcacgtaaa
    11581 atgcttgttt cagaattgtt ttgcggcaag cgaaccgatt cttccagttt aatgcgatga
    11641 attttttaag tctgatcaaa gatcatttcg attttttaaa cttgatacct tcatttttgg
    11701 attgtattga acaaattgcg tgacaaactt tgagagaggg atctgacagt gctaaattgc
    11761 actagagatg ggatgctggg ataggcagtc aggcgtgatg tatcaagcgg ttttttaata
    11821 aggccggttg gaaggtgaaa gttagaaaag gttcaaaacg attccggcgg ctaggcttat
    11881 gagcgcgagc aggctgagaa tggcgagagt ttccttcatg tctttacttt ccttgataag
    11941 gacgagaatc ccaagtcctc cactcgcgca aaggcccgcg atggcggaac caaacgtgat
    12001 gacgccctta agatgcattt ccgcgatcgc gacagatgcc gcgcaatttg gaattaatcc
    12061 gatggcaacg gcaagggcgg gctgaaacag ggatccgtga aaaaagattt tacccaggtt
    12121 ttcttcaccg gccgtttcaa tcaaaaaacc cagggcgatg gaaattaaaa aaatgaaaac
    12181 ggaaatactc gcggtgtgct taagaggaaa aagcagataa gttttccacc agggctcgtt
    12241 gtgtgagtgt gagtggcagg agcagtgcag gtgttcccca acacgaggga ccggggcgtg
    12301 aggggcgtgt gtgtctggcc gatgaaacgc atcaatcaga tagccggcga cggtggcaat
    12361 gatgatttta actccaagaa tatcgagaac cagttgcgcc tgatcttttt gagcgaggat
    12421 cacggggatg gcttcgt
//
LOCUS       DAOSIO010000025        12151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_5262_length_12151_cov_4.317130, whole genome shotgun sequence.
ACCESSION   DAOSIO010000025 DAOSIO010000000
VERSION     DAOSIO010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 12151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12151
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5262_length_12151_cov_4.317130"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3551
                     /locus_tag="PK590_01845"
     CDS             <1..3551
                     /locus_tag="PK590_01845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG23372.1"
                     /translation="IARTIQDILEHFQIPFKVVATIDDLRRMNTQPPPQKGKGMVVYW
                     RGDDALRVDQMLNRALSEKVEDVNRRFRALKKEMIMGLLLPSVNGFLFLKRITGFDLK
                     KAFSILTYRSMDIEDLGLMFLATLIDRELYARGIRPPATQAFDSEGRTGDKLFEGFTG
                     RIGYRWADSRPWVDDRRRYKPEDVHRTLALGSDPADRYRRPAVWEREGIELFLAELAD
                     KERRRIFSRSELREKEVSSKTYQLDIDDTKEGVGAFVHEFGNFLTSSGILANMELWGY
                     QLNPESKLAKFCSTLQDVMENYNEDPLGNLSEVVNDLYKIFHEASWKGLENELGALKE
                     PVEADFTIEYLKHTLQLQEYFYWFLASHLVGVEEIPGKTLWLKETLESVVSISDALRG
                     KVEISEELQSAGQKIELTTDGAALAQVLLNLLRNAQRAVEVKFGKNSPEKGKIRVRIR
                     RGGGSHPRILVTVWDNGVGMSSDVQKKIFSESVTTKETQEGHGFGLMVVAERVKNRLG
                     GRIRFRSKEGEGTVFMLQLPVDINHPLKNISEDTYQKDQSQRSETREREMPSTAKVSP
                     TSSPDGAVSYIAANFPDYKSLDLPSEIFGGNGTDLIAVLIDVAVQSGLSPIVAVKGAL
                     NYARFEGNIDWRNVSDFDLTLYIPALTHHSDSQVLHRQISEGLSRELEKRGHKMSREE
                     TEGLVEVSIYNFRDHYDHVISLDIQPMSALAGNDLLLNYNPFDQYFGRPDAVDGFKQL
                     IDYSFSRTSVLNLAEAQYRDIYKRAKAMRSSSDVRDVQKTFKRLAQLAYWIGDSLRHR
                     KYLEKYQNAFDQLFEDKSNRQRLEDDLDKELPLMDPDLIGRDLLRKSLAVFLNIAPEA
                     EVQRAEVRAVLGPDILKVGEGERIGYAPEFIEGLEKSGFNNDPFYARVLSAALAGKII
                     RQGGMTAEDSASILKALMTAGSKKTGDWVGEVKKILELEDKLGLFAELHRIKKGASLT
                     GVITEGLLQECRVLLTANGQQRISLLIAADTSDKEALNVAAQEMVKREMEWADGEGLD
                     FSGRFEIVIVNARMLGPELRNFSNRQRGALHKVLKGRQSELKDVADFFVISVGEDMFP
                     SLQGSLDTISGTTIVRPDEVERNPKLFGASRFAGVQASTDKVAGGAELLDGRTDLFTP
                     VDRHLRHYRFVVDGLRGLASQLFQAVESLRRIAVAA"
     gene            3752..5449
                     /locus_tag="PK590_01850"
     CDS             3752..5449
                     /locus_tag="PK590_01850"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG23373.1"
                     /translation="MNQKRFHVILVKPSKYDDEGYVIRWWRAVVTSNSLACLVSLTND
                     AGARMILGPDVELVTHIFDETVEKIPVVKLCRTIKKAGERAVVSLVGVQSNQFPRALD
                     LARQFRKEGFSVMIGGFHVSGTTAMLPEKIPELERSADGVTLVAGEVEGNWDRLLRDA
                     YAGKLERKYNFLEVRPDLQGVPGPIMSRENLRYFINTHSSFDAGRGCPFRCSFCTIIN
                     VQGHAMRPRTADDIEKLVRENHRHGVNHFFITDDNFARNPNWETIADRLIYLKEKEGL
                     RSSLMLQTDTMAHKIPRFIEKMTHAGCRRVFIGMESVNPRNLDACGKRQNKISEYRRM
                     LQAWRDHGALTCAGYIIGFPNDTYESIMRDVEFLKKEIPLDLAEFFVLTPLPGSEDHQ
                     KMTRQGTRMDTDLNGYDADHVVMEHPQMSREEWTRAYRDAWSSFYSREHCLTLLKRRK
                     GPRRRLLFSSLIWFRSAFFLTGVHPLLGGYFRSHGRKDRRPGMPLEPIPVYYTKRGIE
                     FLKYSFGMLTTLWDLWWLNRKAQAPENESYTDSAIQPEPVEPTSNFATDKNSSVCAS"
     gene            5477..6265
                     /locus_tag="PK590_01855"
     CDS             5477..6265
                     /locus_tag="PK590_01855"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOG23374.1"
                     /translation="MIDAHLHLQHIPRKRVLESILAEAPAREVDYFFCNAIKLADCEL
                     LSELAAEYDSIIPFYGAHPWEVPNLTEGWDRELESYMAEPGCGVGEIGLDKARDIDFT
                     AQKDVFTRQIELAGQYRKPVMIHVVRAWGEVLPVLRKCAGSTVFLIHAFRGSVEVLEE
                     LVDLGAYVTFSWKLFKQKYGEPVSKVKSLIRIVPEDRLLLETDFPYIGEKVFSTLTAE
                     KYFECLDETYHIAAALLDRDVEDLKNQTRRNGSAFLSLPSSNES"
     gene            6286..7053
                     /locus_tag="PK590_01860"
     CDS             6286..7053
                     /locus_tag="PK590_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011187382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOG23375.1"
                     /translation="MTGRFFCTELLIGKEKVARLKRSFVTIVGLGAVGGYVAEALGRL
                     GVGKIRLVDYDRLETSNLNRNILALESTLGESKVEAMRKRLLDINPACCVETLEVFAA
                     EDSFPSIFSDRPDAVIDAIDSLNPKARLLVYCHENQIPVFSAMGAATRTRIQSIQTGD
                     LFKSRGCPLAREVRKRLRKSGIQSGIFCVYSDEPARQVPNVLPNEEDNYPRGRKRAKL
                     GSLPTIPAIFGLTLAHHAAEYLCGSFDTSVHEVSGLA"
     gene            7352..7552
                     /locus_tag="PK590_01865"
     CDS             7352..7552
                     /locus_tag="PK590_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOG23376.1"
                     /translation="MIQGTVKWFNNSKGYGFISREGGADVFVHFSAITGDGYKSLEEG
                     QKVQFEVTTGPKGEQASNVSKI"
     assembly_gap    7569..7578
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7669..9090
                     /locus_tag="PK590_01870"
     CDS             7669..9090
                     /locus_tag="PK590_01870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOG23377.1"
                     /translation="MDSSQFWLIVSIALYMVVMLGIGVWTSRKIHDTHDYILAGKRLG
                     WWLSIGTIFATWFGAETCMGSSRTAYEKGILGVIADPFGAALCLILAGLFFAKFFHDK
                     NYKTLVDYFDQRFGRWAAGLLSVLYIPVYVGWVGAQLLAFGIILNSVTGIEPMTAIII
                     STAVVIVYTYMGGMWAVSVTDLVQMLILIAGFLVLFPILYFEVGGFGSLRAQLPPEMF
                     HFWPRQGSTLDWLMYLQAWMLVGLGSLPGQDLFQRLMSPRSAHVAKWSALIAGVLYVV
                     IGLFPVLMGMMGRIALPTVGRSESILIDLARAYLPLPMIALMLGALLSAIMSTVSAAL
                     LAPASIIGHNIIPFFYPRISESAKLQWCKNGIWIIGVISFFVAIYFQNVYTLCTQSWG
                     ILLVGVAAPLIFGVFWKKTNTAGALAGAVAGTVSWIVLALALSDDYPTHLFGFAISCV
                     TLVVVTLLTSFFPRSHSAQAKIG"
     gene            9280..9867
                     /locus_tag="PK590_01875"
     CDS             9280..9867
                     /locus_tag="PK590_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOG23378.1"
                     /translation="MTFLWILLGVVVLTILAVVSIYNALVMLKNRFKNAFAQIDVQLK
                     RRYDLIPNLVETAKGYLQHERVTLEAVIQARNQAVSAAQKAASIPGDPQAMGNLKTAE
                     TGLSGALGKLFALSESYPDLKANQTMMSLMEELTSTENKISFARQAYNDSVMAYNINR
                     EKFPNVLFASSFGFMPAALLELEGAEERKKPDVKF"
     gene            9886..11898
                     /locus_tag="PK590_01880"
     CDS             9886..11898
                     /locus_tag="PK590_01880"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOG23379.1"
                     /translation="MNFFEQQDKARQRTFVLLFFYVLAVLATIISVYGAVRLCWYFMP
                     VSRKSPLYMPVYDTVPVDFWDLNCFLTVTGIVLLVVLTGTLIKIAQFSKGGVAVAKML
                     GGEQILPGTKDPDERKVLNVVEEMSIASGVPVPAVFLLKNEYGINAFAAGFSTQDVVI
                     GVTRGAVSLLSRDELQGVIAHEFSHIFNGDMRLNMNLTGFLNGLLLIALTGKTLLRGV
                     RGGHGQSSNRGSGATVFVILLFALLLLVVGYIGVFFCNWIKSAVSRQREYLADASSVQ
                     YTRNPSGLAGALKKIAGLPDGSFIRQTRAEEVSHFFFSDGVAVFGGTIFSTHPPIHAR
                     IRLLDPSFDGKYPVVRSIAPAETRAFRSPRPANPHLGEELAKWEKKMSLSQTPRTDLG
                     HESLAESVVFQVGVITDNHLNRASEIREALPALFREAVTSVTGSIALVYGLLIDRRNT
                     DVRSHQLEYLARHDDPSVYKMLLRLLPDFSSLKTEWCLPLLDLATPTLKTQTSERYEK
                     FREHVETLIHEDQSVSVFEFVLKQVVLHRLDLFFGKAKKTTVVYSDWKPILPDCEILL
                     SYIAGAGSFFRPLVEKSFFKAVESLGQDFSWKLIPFELLTYDQIVGALKKMAAASPKL
                     KEQFLKACVHCIEHDEQLDIRELELLRAFASTLDCPIPPFIPQAEN"
BASE COUNT         2790 a         2731 c         3405 g         3215 t
ORIGIN      
        1 agatcgctcg aacgattcaa gacattctcg agcattttca gattcctttc aaagtggtgg
       61 cgacgatcga tgatttaaga cgaatgaaca ctcaacctcc gccgcaaaaa ggaaaaggga
      121 tggtggtgta ctggcgagga gatgatgctc ttcgcgttga tcaaatgttg aacagagcct
      181 taagtgaaaa agtagaagac gtaaaccggc gctttcgtgc cctcaagaag gaaatgatca
      241 tggggctttt gcttccatct gtgaatggtt tcctgttttt aaagaggatc accggatttg
      301 atttaaagaa ggctttttcg attttgacat atcgttccat ggatatcgaa gatttgggtc
      361 ttatgtttct ggctaccctg attgaccggg agctttatgc gagaggaatc aggccgccag
      421 ccacgcaagc gtttgattcg gaaggccgga caggtgacaa acttttcgag ggattcacag
      481 gaaggattgg ctaccggtgg gcggattcac gtccctgggt ggatgatagg aggcgctata
      541 aacctgaaga tgttcatcga acgcttgctc tgggatcgga tcccgcggat cgttaccgcc
      601 gccctgcggt atgggagcgt gagggaatag aattatttct tgcagaactt gcggataagg
      661 aacgccggag gattttttca cgctcggaac tgcgggagaa ggaggtttct tcaaaaacct
      721 atcagttgga tattgatgat acgaaggaag gtgttggagc gttcgtccat gaattcggca
      781 attttttgac gtccagcggt atcttggcga acatggagct gtggggatat cagctgaatc
      841 ccgaatcaaa actggcgaag ttttgctcga cactgcaaga cgttatggag aattataatg
      901 aagatccgtt agggaattta tctgaggttg tgaatgattt atacaagatt tttcacgagg
      961 cctcttggaa aggtctggag aatgagcttg gcgcattaaa ggagccggta gaagccgatt
     1021 tcacgattga atatctaaaa cacactctcc aactgcaaga atatttttac tggtttctgg
     1081 cttcgcatct tgtgggagtt gaggagattc cgggcaagac cctttggctt aaagaaacgc
     1141 tagagtccgt tgtgagtatt agcgacgcgt tgcgaggcaa agtggaaatt tccgaagaac
     1201 ttcagagtgc gggtcagaaa attgagctga cgacagatgg ggcagctttg gcccaggtgt
     1261 tgcttaacct tctgaggaat gcccaaagag cggtagaagt gaagtttggg aaaaattcgc
     1321 cagaaaaagg aaaaattcgt gtgaggatca gacggggagg tggttcccat ccccgtattt
     1381 tggtgaccgt ttgggataat ggagtgggga tgtcgtctga tgtgcagaaa aagatttttt
     1441 ctgagtcggt aaccaccaag gaaacacaag aagggcatgg gttcggactt atggttgtgg
     1501 ccgagcgagt caaaaatcgc cttgggggca ggatcagatt tagatcgaag gaaggcgagg
     1561 ggactgtatt tatgttacag ctacccgtgg atatcaatca cccgctgaaa aatatctctg
     1621 aagacactta tcaaaaggat caatctcaac gttcagagac aagagagagg gagatgccgt
     1681 ccacggctaa agtttctccg acctcctccc cagatggcgc agtttcttac atcgcggcaa
     1741 atttccctga ttataagtca ttagacctac cgtcagaaat ttttggaggg aacggcactg
     1801 atttgatcgc agtgctgatt gatgtggcgg tgcaatcagg cctttcaccg attgtggcgg
     1861 tgaaaggcgc tctgaactat gcccggtttg aagggaatat tgattggcgg aacgtgagcg
     1921 attttgattt gaccctttac attccggcac ttacgcatca cagtgattcc caggttttgc
     1981 acaggcagat ttctgaaggg ttgtcgcgtg agcttgaaaa gagaggacat aaaatgtccc
     2041 gcgaggagac agagggactt gttgaagtct cgatctataa tttcagagat cattatgatc
     2101 acgtgatatc gcttgatatc cagcccatga gcgctctggc cggtaacgat ttattgttaa
     2161 attataatcc ttttgatcaa tatttcggac gaccggacgc ggtggatggt tttaaacagc
     2221 tgattgatta ttcattttct cgaacctcgg tactgaatct tgcggaggcc caataccggg
     2281 atatttacaa aagggcgaag gcgatgcgct catcctcaga tgtccgggat gtgcaaaaga
     2341 cattcaagcg gttggctcag ttagcctact ggatagggga ttctcttcgc cacagaaagt
     2401 atttggaaaa atatcagaac gctttcgacc agctctttga ggacaaatcg aatcgccagc
     2461 gattggagga tgatcttgac aaggaactgc ccctcatgga tcccgatttg atcgggagag
     2521 acctattgcg aaagtctctg gcagtgtttt taaatattgc cccggaggcg gaagttcagc
     2581 gtgctgaggt tcgtgccgtc cttggtcctg atattttgaa agtgggagag ggtgagagga
     2641 ttggttatgc tccagagttc attgaggggc ttgaaaagtc aggattcaac aatgatcctt
     2701 tttacgcaag ggttctttcc gcggctttgg ccggaaaaat tattcgacag ggagggatga
     2761 cggcggaaga ttcggccagt atcttgaaag cattgatgac ggcaggctca aagaagacgg
     2821 gggattgggt tggagaggtg aaaaagatcc ttgaactgga agacaagttg gggctttttg
     2881 ccgaacttca ccgtatcaag aaaggtgctt cgcttaccgg ggttattacc gagggacttc
     2941 ttcaggagtg ccgagtttta ttgacagcga atggtcaaca gcgtatcagc ctcctcattg
     3001 ctgcggatac ttcagataag gaggccctta acgttgcggc tcaagaaatg gtgaagcgtg
     3061 aaatggaatg ggctgacgga gaggggcttg atttttcagg gcgctttgag attgttatcg
     3121 ttaatgcgag gatgttgggt cctgaacttc ggaatttttc gaaccgccag cgaggtgccc
     3181 tgcacaaggt cctcaaggga cgacaatccg agcttaaaga cgtagctgat ttttttgtca
     3241 tcagcgttgg cgaagatatg ttcccatcgc ttcaagggtc gcttgatacc atcagtggaa
     3301 cgacgatcgt gaggcccgat gaagttgaaa ggaatccgaa gctatttgga gcctccagat
     3361 ttgctggagt ccaggcttca acggacaagg tggctggagg cgccgaactt ctggatggaa
     3421 gaaccgatct tttcactcca gtggaccggc atctgcggca ttatcggttt gtggtagacg
     3481 gtctgcgggg tcttgcttca caactttttc aggcggtgga atcgctccgc cgaattgcgg
     3541 tcgcggccta atatactctt caattcccgg acagagtgga cgaggacgcg actctttgat
     3601 gcggacaaag cattttgctt cagggcccgg cctttcttgc agttcctcat gcggggcggt
     3661 gtgatttgag gtataatgcg tgccctgacg tttacgaaat caatcttaag cgcctactcc
     3721 aggaacagac cagattaaaa caggatattt tatgaatcaa aaacgtttcc atgtgatcct
     3781 tgtcaaaccc tccaaatatg acgatgaagg ctacgtaatc cgctggtggc gcgctgtagt
     3841 caccagcaat tccctcgcct gcctggtttc tctcacgaac gacgcggggg caagaatgat
     3901 tctcggtcct gacgtagagc tggtaacgca tatttttgac gagaccgtcg aaaaaattcc
     3961 ggtggtgaaa ttgtgccgga cgatcaaaaa ggccggagaa agggcggtgg tcagtcttgt
     4021 cggagtccag tcgaatcagt ttccaagggc tctcgacctt gcccggcagt tccggaaaga
     4081 gggtttttcc gtgatgatcg gcggttttca tgtgtcgggg accacagcca tgcttccgga
     4141 aaaaattcct gagctcgaac gttcggcaga cggtgtcacg ctggtcgcgg gggaggtcga
     4201 ggggaactgg gatcgtcttc tccgggacgc ttacgcgggc aaacttgaaa gaaaatacaa
     4261 ttttcttgaa gtcagaccgg atctccaggg tgttcccggg cccatcatgt ctcgtgaaaa
     4321 cctccgttat tttatcaata ctcacagcag ttttgacgcg gggagaggat gcccgttccg
     4381 ctgttccttt tgtacgatca tcaatgtgca ggggcacgcg atgcggccgc gtacggctga
     4441 cgacattgag aaactggtcc gtgaaaatca tcgacacgga gttaatcatt tttttatcac
     4501 ggatgataat ttcgcgcgga atcccaattg ggaaacgatc gcggatcgct tgatctatct
     4561 taaggaaaag gaagggttgc ggtcttcgct gatgttacag actgacacga tggcccacaa
     4621 gattccccgt tttatcgaga aaatgacgca tgccggctgt cgccgtgttt ttatcggtat
     4681 ggaaagtgtg aatccccgaa atctggacgc ctgcggcaaa cgccaaaaca agatttcaga
     4741 gtaccggcgc atgctccagg catggcgtga tcatggggcc ctgacttgcg ctggatatat
     4801 tatcgggttt ccgaacgaca cgtatgaatc gatcatgcgt gacgtggagt tcctgaaaaa
     4861 ggaaatccct cttgatctgg ccgagttttt tgtcctgacg ccgcttccgg gttcggagga
     4921 tcatcagaaa atgacccggc agggcacccg gatggacacg gatctgaatg gttatgacgc
     4981 ggatcacgtc gtcatggaac atccccaaat gtcgcgggaa gaatggacgc gcgcttaccg
     5041 ggatgcctgg agctcttttt acagccggga gcattgcctg accctcttga aacggagaaa
     5101 agggcctcgc cgtcggttgc ttttttcttc gctcatctgg tttcgcagtg cttttttcct
     5161 gaccggagtc cacccacttc tggggggata cttcagatcg cacgggcgaa aggatcgccg
     5221 gcccggcatg ccgctggaac cgataccggt ttactatacg aagcggggga ttgaattctt
     5281 aaagtacagc tttggaatgc tgacaactct ctgggatctc tggtggctca accggaaagc
     5341 ccaggctccc gagaacgaaa gttacacaga ttcagcaatt cagcccgaac cggtcgagcc
     5401 aacttctaat tttgctactg acaaaaattc aagtgtctgt gcctcctaga cgggagtttt
     5461 ttgaaaggaa attgtattga ttgacgccca tcttcatctt cagcatattc cccggaagcg
     5521 tgttcttgaa tcgatccttg ccgaagcgcc ggccagggaa gtggattatt ttttctgtaa
     5581 tgccatcaag cttgcggatt gtgagcttct gagcgaactg gcggctgagt acgactccat
     5641 cattcctttt tatggtgcgc atccgtggga agtccctaat ttgacggagg gttgggatcg
     5701 ggaactggag tcttatatgg cggaacccgg gtgtggcgtc ggagaaatcg gactggataa
     5761 ggcgcgggac attgatttta cggctcaaaa agatgttttt acccgccaaa tcgaacttgc
     5821 cgggcagtat cgaaagccgg ttatgattca cgtggttcgg gcctggggtg aggtccttcc
     5881 ggttcttcga aaatgcgcgg ggagcacggt ttttctgata cacgcgtttc gcggttccgt
     5941 ggaagtgctt gaggaattgg tcgatcttgg cgcttatgtg actttttcct ggaagctttt
     6001 taaacaaaag tacggagaac ccgtctcaaa agtgaaatcc ctgatccgga tcgttccgga
     6061 agatcgactg cttcttgaaa cggattttcc ctacatcggg gaaaaagttt tttcaacgct
     6121 gacggcggag aagtactttg aatgtctcga cgagacttac catatcgccg cggcattgct
     6181 ggatcgggac gttgaagatc tcaaaaatca gacccggcgg aatggatcgg cttttttaag
     6241 cctgccatcc tcgaatgaaa gttagggggg gaatccttga cggagatgac ggggcgattt
     6301 ttctgtacag agcttctgat cgggaaggaa aaagtcgcgc gactcaagcg atcgttcgtg
     6361 acgattgttg gtttgggcgc ggtcggagga tatgtcgcgg aggctctggg cagactgggc
     6421 gtgggaaaaa ttcggttggt ggactatgac aggcttgaga caagcaacct caaccggaac
     6481 attctcgcgc ttgagtcgac gctgggggag agcaaagtcg aggcgatgag aaaacgactc
     6541 ctggatatca atccggcctg ctgcgtcgag accctggaag tttttgcggc ggaagattca
     6601 tttccctcga ttttttcgga ccgtccggat gcggtgattg acgctattga ttccctgaac
     6661 cccaaggccc gtcttttggt ttattgtcac gagaatcaaa ttccggtctt ttccgcgatg
     6721 ggcgcggcaa cccgaaccag gatacaatcg atccagacag gggatctttt taaatctcgg
     6781 ggatgtccgc tggcgcggga ggttcgcaag cggcttcgaa aaagtggaat tcaaagcgga
     6841 attttttgcg tttattccga cgaaccggca cgccaggtcc cgaacgttct tcccaacgaa
     6901 gaagataatt atccgcgggg aagaaaacgc gccaagcttg gaagtttgcc gacgatcccc
     6961 gcgatctttg gtcttacgct cgcccatcac gcggcggagt atttatgcgg aagttttgac
     7021 acgtcagttc acgaagtgag cggtttggcc tgaagactga tcggccaata aatttttctt
     7081 tcccatggga ttgacaaatc ttcaaagatg aggcaaactt ccgcctgtag taagcaatga
     7141 gggtgaacgt tgtatggccg caaggcaaaa cgatccgcct cgcggttttt ttgttttacc
     7201 cccgtttttc ggtctttgac cggagggcgg gccagttcca aaaggtcgtg gttgatcttg
     7261 atcacgcagg aaagttggtc tggtcatttg ttactatggg aacgggaaag tcccggtctc
     7321 aaaattattt tgcaaaggag caacatcaac catgattcaa ggtaccgtga agtggttcaa
     7381 taattcgaaa ggctatgggt tcatctcccg cgagggtgga gcggatgtgt ttgtgcattt
     7441 ttccgccatt acgggtgatg gctataaatc gcttgaggaa ggtcagaagg tccagtttga
     7501 agtgaccact ggccccaagg gcgaacaagc ctcgaacgtt tcgaagattt aatcacttcg
     7561 agaggttgnn nnnnnnnnaa aggcccgaca gcttaagctg ccgggccttt gtgtgtctgc
     7621 gagcacgttt ttcctcacaa accacaaaat ttttggagta aaagagccgt ggattcttct
     7681 caattttggc tgatagtaag tattgctctc tatatggttg tgatgcttgg gatcggggtc
     7741 tggacaagtc gaaaaattca cgacacccac gactatatcc tggctgggaa acggctcggc
     7801 tggtggctct cgatcgggac gatttttgcg acctggtttg gcgccgagac ctgcatgggt
     7861 tcctcccgaa cggcctatga gaaggggatc ctcggggtta ttgccgatcc gtttggcgcg
     7921 gctctctgcc tgattctggc gggacttttt ttcgcgaaat ttttccatga caaaaattat
     7981 aaaacactgg tggattattt tgaccagcgg tttggccgat gggcggcagg acttttgagt
     8041 gtgctttata ttcccgttta tgtcggatgg gttggcgccc aactgctggc tttcgggatc
     8101 attctgaatt cggtaaccgg gatcgagccg atgaccgcca tcatcatttc gaccgctgtg
     8161 gtcatcgttt atacctacat gggagggatg tgggccgttt cagtaacgga tcttgtgcag
     8221 atgctgatcc tgatcgcggg atttcttgtt ctctttccaa tcctttattt cgaagtgggc
     8281 ggatttggat ccttgagggc acagcttcct ccggaaatgt tccatttctg gccgcgccag
     8341 ggttcaacgc tcgattggtt gatgtatctt caggcctgga tgctggtggg gctcggttcg
     8401 cttccggggc aggatctttt tcaacggctc atgtcgccgc gaagcgcgca cgtggcaaaa
     8461 tggagcgctt tgatcgcggg agtcctttac gtggtgatcg gactttttcc cgttctgatg
     8521 gggatgatgg gacgaattgc gttgcccacg gtgggccgca gtgaatccat cctgatcgac
     8581 cttgcccggg cctatttgcc gcttccgatg attgccctta tgctcggagc gctcctttcg
     8641 gctatcatga gtacggtcag cgccgcgctt ctggcgccgg ccagcattat cggccacaac
     8701 atcatcccgt ttttttatcc ccgtatttct gaatcggcca agcttcaatg gtgcaagaac
     8761 gggatatgga taatcggcgt catctctttt tttgtcgcca tctatttcca gaatgtttat
     8821 accctttgta cccagtcctg gggtattctc ttggtcggag tagcggcgcc gcttattttt
     8881 ggggtgttct ggaaaaaaac caatacagcg ggcgcgctgg caggggcggt cgcggggacg
     8941 gtatcctgga ttgtgttggc gctggccctt tccgacgatt atcctacgca cctttttggt
     9001 tttgccattt cgtgtgtgac gcttgtggtc gttactcttt taacttcatt ttttccgaga
     9061 agccattctg cccaagcgaa aatcggttga tctcccttta agaaggaagg agaatgcccc
     9121 cctgacctgg cgaatggcgt ggtagggtaa agattttggt ctgggattgt cgaggcagac
     9181 aggtttgccc gcgcgggagc ttgacgaaat tcattgttca gtcataggat ttcgagggtg
     9241 ccggattttt caggtaaaaa atatttcaag gagggatgta tgacatttct ttggattctg
     9301 ctgggtgttg ttgttttaac cattcttgcc gtggtgagta tttataacgc gcttgtgatg
     9361 ctcaaaaacc ggttcaagaa tgcctttgcc caaattgacg tgcagttgaa acgtcgttac
     9421 gatcttattc ccaatcttgt tgaaacagcc aagggttatc ttcagcatga gcgagtgacc
     9481 cttgaggcgg taatccaggc gaggaaccag gcggtcagcg ccgctcaaaa ggccgcgtcc
     9541 atccccggcg accctcaggc gatgggcaat ctgaaaaccg ctgaaacggg gttaagcggg
     9601 gcgctcggaa agctttttgc gctgtcggag tcttatccgg atcttaaggc caaccagacg
     9661 atgatgtcgc ttatggaaga gctgacctca accgagaaca agatctcgtt tgcgcgacag
     9721 gcctataacg acagcgtcat ggcttataat atcaaccgcg aaaaatttcc caatgtcctt
     9781 tttgcgtcgt ccttcggttt tatgcccgcc gctcttcttg aactggaagg tgcggaagaa
     9841 aggaaaaagc cggacgtgaa gttttaaccc gtttccctgt cagcgatgaa tttttttgaa
     9901 cagcaggaca aagcccggca acggacgttt gttctgctgt ttttttatgt cctcgcggtt
     9961 cttgccacga ttatttcggt ttatggggcc gtgcgtctgt gctggtattt tatgccggtt
    10021 tctcggaaga gtcctctcta catgccagtc tatgacacgg tccctgttga tttctgggat
    10081 ctgaattgtt tcctgaccgt tacggggatt gtgttgctgg tggttttgac ggggaccttg
    10141 attaagatcg cgcaattttc caaagggggt gttgcagtcg caaaaatgct tggaggtgaa
    10201 caaattctgc cgggcacaaa ggatcctgac gaacgcaaag tcttgaatgt ggttgaagag
    10261 atgtcgatag catccggcgt gccggtcccc gcggtttttc tcctgaaaaa cgaatatggg
    10321 atcaacgcct ttgcggcggg tttttcaacg caagacgtgg tgatcggtgt cacccgcggg
    10381 gctgtcagtc ttctttcgcg ggatgaactt cagggtgtca tagctcacga gttcagtcat
    10441 atttttaacg gtgacatgcg gctcaatatg aatctgaccg gattcctgaa tgggttgctt
    10501 ttgattgcgc tgacaggcaa gaccctcttg cgtggggtca ggggcggtca tggccaatcc
    10561 agcaatcggg gaagcggcgc gactgttttt gtcattctgc tctttgccct tctccttttg
    10621 gtggtgggat atatcggggt ttttttctgc aactggatca aatcggctgt ttcacgtcag
    10681 agggaatatc tggccgacgc gtcgtcagtt cagtacacgc gcaatccctc gggtctcgcg
    10741 ggagcgctca aaaaaattgc gggccttccc gacggttctt ttattcgcca gaccagggcg
    10801 gaagaagtca gccatttctt tttttccgat ggtgttgcgg tgttcggggg gaccatcttt
    10861 tccacgcatc ccccgattca cgccaggatt cggttgctgg atccttcatt tgacgggaaa
    10921 tatcctgtcg ttcggtcaat cgctcccgcg gagacaagag cgttccggtc ccccaggccc
    10981 gcaaaccctc acctcggcga agaacttgca aaatgggaaa agaagatgtc tctttctcaa
    11041 acgccgcgaa ccgatttggg acatgaatct ctcgcggaat cagttgtttt tcaggtggga
    11101 gtgattacgg acaatcatct gaaccgggcc tcggagatcc gggaagcatt gccggcgttg
    11161 tttagggagg cggtgacgtc agtgactggt tcgatcgcgc tggtctacgg acttttgatc
    11221 gaccggagga atacggatgt tcgtagccac cagcttgagt atctcgcccg gcatgatgat
    11281 ccctcagttt ataaaatgct tctcaggcta ctgcctgatt tttcaagtct gaaaaccgaa
    11341 tggtgtttac cgcttttgga tcttgcaact ccgacgctga aaacccagac gtccgagcgc
    11401 tacgaaaaat tccgggaaca cgtggagacc cttattcacg aggatcaatc ggtttcggtt
    11461 tttgaatttg tgctgaagca ggtggtgttg catcgtctgg atctcttttt tggaaaggcc
    11521 aaaaagacga cggtggttta ttcggactgg aaacccattc ttcccgactg cgaaattttg
    11581 ctgtcataca tcgcgggggc gggcagtttt ttcaggcctc tggtggaaaa atcttttttt
    11641 aaagcggtcg aatcattggg acaggatttt tcatggaaat tgatcccgtt cgaactacta
    11701 acgtatgacc agattgtcgg tgcgttaaaa aaaatggcgg cggcgtctcc caagctcaag
    11761 gaacagtttc tgaaggcgtg tgttcattgt atcgaacatg acgaacagct cgacatccgt
    11821 gaactggaac ttctgcgggc ttttgcctcg accctggatt gtccgatacc gcctttcatc
    11881 ccccaggcag aaaactgaca cccctgagtc gggcccgtaa tgatgccaat ttctgagaat
    11941 gggccgtcaa gaaagaacgc catgagattt cggctcgaaa ccgtggccct tgattttccg
    12001 cgatgaaaaa aaacatcgcg gagcccgaca gatatttttc ccttccggac gctctcagtt
    12061 tccctggcgg tgctttggga atttcgggct tttgcgtagt ttgccgggaa accgatcccg
    12121 ttgaacattt tcagtttgat gcgtggtcgt g
//
LOCUS       DAOSIO010000026        12140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_5270_length_12140_cov_4.270666, whole genome shotgun sequence.
ACCESSION   DAOSIO010000026 DAOSIO010000000
VERSION     DAOSIO010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 12140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12140
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5270_length_12140_cov_4.270666"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="PK590_01885"
     CDS             complement(<1..1110)
                     /locus_tag="PK590_01885"
                     /inference="COORDINATES: protein motif:HMM:NF016827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23380.1"
                     /translation="MALQHFTVMVFCRMKQNLQQFQQQKLLLSPQMRQYLRLLRLPVA
                     DLRQAVHAVLEENPMLEEANLPLDPELATAESPQEPHDEENDPSPVISGDWEGAEPLD
                     FLRPSVDFSKRPASVSQKLKDFQDSLLTPPESLFDYLEWQLRFLDLSGTEQRIAKQII
                     GNITEDGYLAATTEEIAKAAQTDPAAIGPVLTKIQELDPPGVAARDLKEALFLQLQRK
                     GPGAALAKEMVLHHLELVARKDYKSLAKIFHKDERAIQNAIDMISRLEPKPGRSFYAE
                     EPIAVTPDVTVSTKEGTTGGLEIRMNEEFIPRLTLNQEYRKMLRGRDLDEKTARFLKE
                     KLAGGIDFIKALELRKNTLQGITEEIVKAQPLFFTR"
     gene            1276..2112
                     /gene="accD"
                     /locus_tag="PK590_01890"
     CDS             1276..2112
                     /gene="accD"
                     /locus_tag="PK590_01890"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOG23381.1"
                     /translation="MSQAPALMITRGLRKKSMPEGLWTKCPSCQTVVFNKTLKENLLV
                     CAKCNYHFIMGAWERIYHLVDDKTFREIAANLQPGDPLKFSAVKSYSEKVKADQKRTG
                     LKEACIIGEGKMNGHPVALGVTDSRFIMGSMGSVVGEKVTKLTEYALENKLPLIIVSG
                     SGGGARMHEGALSLMQMAKTSVALTRYQKERFPFISILTNPTMGGVMASFASLGDIII
                     AEPRALLGFAGPRVIEQTIRQKLPPDFQTSEFFVEHGMVDMVIDRRDLKQKITTILEI
                     LC"
     gene            2131..3237
                     /gene="ugpC"
                     /locus_tag="PK590_01895"
     CDS             2131..3237
                     /gene="ugpC"
                     /locus_tag="PK590_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOG23382.1"
                     /translation="MANVALKNVSKIYKGDVKAVNGVNIDIQDREFVVLVGPSGCGKS
                     TTLRMIAGLEEISEGEIFIENTRVNDVPAKNRDIAMVFQNYALYPHMTVYENLAFGLK
                     LRRYPRREIERRVHEAAEILGLSLDMLQRRPKELSGGERQRVAVGRAIVRKPKVFLFD
                     EPLSNLDAKLRGQMRMELQKLHNRLQSTMIYVTHDQIEALTLGDRVVVMNKGVVQQIA
                     DPINLYERPINRFVGGFIGSPPMNFLKGTIAAENGNYFFSDKSLKLRILPHYYPRISA
                     YKNQPVLMGIRPEDIYDKIFAQDARPEFTTTATVDVVEPMGAEIFLYLTACSNSFVVR
                     VSNQDTATMNQDLQVVFDMSKAHFFDPQTGVVIF"
     gene            3251..4696
                     /locus_tag="PK590_01900"
     CDS             3251..4696
                     /locus_tag="PK590_01900"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOG23383.1"
                     /translation="MGVPARRHLVFLAGVGVFAFSVFLIFQSLAQLRTTLHPEVYLQR
                     LSLIFAALMFPILTTWWRFKWIGVNILMIFLVIPLVLFVVSVTKISVFFWVYLAYAIL
                     MLVLYRTEHQFENQLSMILIEEEKLQDEINNLEVVFKAKGEGISTFFEKYSTYYNLRK
                     LAEELTMSLSWTELAQKTVDRAMDFIPRGDYAKMTLARSDERNLPVVASRRMRPVGRW
                     TAKQGDLFDFWCIKNRKRLIVMDSQDDFRFALRENDKRENLRSLIVAPIFFENRVSGT
                     LAIHATRPNVFTHEDLRLLDAIATLASSAISNAMLYEKTEQLAIKDSLTGLFVRRYFY
                     ERLKEEHRRALLNQKTMSLIMCDLDHFKECNDRFGHQGGDLMLVQLAEILGQSLKNAL
                     LARYGGEEFAILLPETTKAEAFKIAEGLRKTVETTPFHIRREKVGMTVSAGVSNLPDD
                     TLDIELLVHKADQALYRAKREGRNKVCVSTS"
     gene            4678..6063
                     /locus_tag="PK590_01905"
     CDS             4678..6063
                     /locus_tag="PK590_01905"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOG23384.1"
                     /translation="MRKHVLILLFQLGLAAIILHQTRELILVYYLLAPVLLLPAFYFY
                     KERSRGMLLVALLVAAAVNLRYFFLDPMPRLFLFWGGQAVFFMLSCAYSRELRIALDY
                     ASSKKNEVRKNLENLRAKFQTRISSLKHLEAQVGSLMNLFEIARDFSECMDFATLSDF
                     LLKKLRSELSFQRMRIFILPLTPASREKGGVKLYEMTLDGVQEIERELSDEEKTDFDS
                     MALTKNMVRREKMWFFPILEAEKLTAVFSIDGVQDEDLARVEVLSAYLVLLIKKIQLY
                     ETVRELAIIDELTQVFVRHHFVERLEEEVRRSIRFKLPLTVLMLDIDHFKRYNDEFGH
                     LVGDATLKEVADLLKKNLRRVDLVGRYGGEEFVVAMPETRMENACEVAERIRSNIARH
                     DFRVYNDRTKVTVSQGLRVFDAASIREPEKVDVPKLVLELIHHADESMYRAKEEGRNR
                     VCVYKEPHNLL"
     gene            6060..8369
                     /gene="lptD"
                     /locus_tag="PK590_01910"
     CDS             6060..8369
                     /gene="lptD"
                     /locus_tag="PK590_01910"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HOG23385.1"
                     /translation="MSQCFFKRIAFFSTLLLALTALIPVSFSQDDTPTTPSEKQPVTA
                     PRNLPYPTVRDQSRAEVEAVADTLEYQKDSRKLVARGNAVVTHQNIKLLADYAEVETE
                     TKKSFAKGHVIIFENETPILTGDEVYYDFSTRQASMPGAKAIQTPWYARGEEVQQVKQ
                     GEIHVRHGGVTTCNLENPHYEIRCKKATLYSGVKIRMVNVTIHVLGKPVFWLPYLEVP
                     LNWNIPFQVRVGSSDQYGTYIELSKGITFNKYLSGKWHADWRSKRGFGGGWDQNYDFG
                     KYAKGDVKLYWTQDKRAPTPNHYDADGKLNPYELREDRDRGRITWRHRTDFSEYTNII
                     MRYHRVADGYFLKDFAENEYRAEMEPHSFVTATHNTQRWGAMVHAEKKMNSHENIIER
                     LPQVRLDWKNQPLMGDKVYNVSRLQFDNLGKKIGYSDYNEDVIRVDGYSRLIAPVKLG
                     DVKMTPYLGYRATEYSRDLRTDGAHFRQVLEYGLDVRTQAYKTFDVTFEKAGIEVNQL
                     RHIFEPNVRVQGTDSTLSYEKIAHFDTVDMIDDASEIVFGMDNRLQTKRVANGRNQRV
                     DIVSMNTYAHFAISPQDPNIQGAKFTLVENELTLRPYDWLQFQHRLEYDAARNYIKRC
                     NNDIVIRHQRLKLTFGHRFIHRQYDYTQNLDIDTSQQFVFDGEYKLNDLWTLAGYIRW
                     DQKGSSLQEWQVSASRDLHDFILDFGYNVRDSLINSKNNELFFNFRMKDFPGMDFGLG
                     GGRATFSPPRIGETVEGASQDRSLLSKSF"
     gene            8427..9725
                     /locus_tag="PK590_01915"
     CDS             8427..9725
                     /locus_tag="PK590_01915"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOG23386.1"
                     /translation="MKLAVIGSGYVGLVTGACFSNLGHDVVCVDNDPEKIRTLKAGKI
                     FFYEPGLHEMVSETVAAGRLSFTTEIKKVVNRCEVIFICVNTPSLPDGGADLSSVEKV
                     AKVLAKNLRKYCLIVAKSTVPVQTGEWIRKTVKRHVRPGVTFDIASNPEFLREGSAIH
                     DFLKPDRVVVGIDSRRAEKIFRDLYASMKAPLIVTDIKSAELIKHASNSFLAAKISFA
                     NGLSRICDIVGADVDQVTLGMGLDPRIGPQFLKAGVGFGGACFPKDLKAFLHISDKLG
                     ARFDLLQEVIRINESQAMYFVRKIVKEMKSLKEKTIGVLGLAFKSDTDDMRNAPSLAV
                     IPELQKEGARIQAYDPQAMEKARPHLKRVQYVAGLYEAVKGADAVVLLTEWKKFQNMD
                     CRKARKLMKGKYFFDGRNMFEPSEMKRYGFRYVSIGRPSK"
     gene            9779..10510
                     /locus_tag="PK590_01920"
     CDS             9779..10510
                     /locus_tag="PK590_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOG23387.1"
                     /translation="MKGIILAGGLGTRLYPLTKVTNKHLLPVYDRPMIYYPIETLVKS
                     GIRDILIVTGGNNAGDFLKLIGNGKQFGLKHINYTYQEGEGGIAHALSLAEHFADGKK
                     IVVILGDNICEANISSYVQSFRKQPDGARILLKKVPDPERFGVATIRGSRITKIVEKP
                     KKPESAYAVTGIYMYDKSVFEFIKTLKPSRRGELEITDVNNWYLKRGAMEFDVLPGYW
                     TDAGTFESLFRANALVARHKGVGRS"
     gene            10507..11484
                     /gene="rfbB"
                     /locus_tag="PK590_01925"
     CDS             10507..11484
                     /gene="rfbB"
                     /locus_tag="PK590_01925"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOG23388.1"
                     /translation="MKKILVTGGCGFIGSNFIRFFLRERPRWAVINLDKISYSGNPKN
                     LQDLKKHPRYEFVRGDICDKKRVASVMAKASAVVHLAAETHVDRSIDSADEFIRTNVL
                     GSTVLLEASLARGIERYVHMSTDEVYGSVPRGKSNELSPLAPNSPYAASKAAADLLVR
                     SFQVTHKHPAMIVRCSNNYGPYQFPEKVIPLFVTNLLEDKPVPLYGEGKNCREWIFVE
                     DACRAILTVLEKGKPGGIYNVGSGQERSNLQLTRAILKQMEKKDSMIRRVQDRPGHDF
                     RYALDFGKIRKLGFKPWWNFERGLKTTVDWYKVNEGWWRPLKKDRFTVK"
     gene            11442..>12140
                     /locus_tag="PK590_01930"
     CDS             11442..>12140
                     /locus_tag="PK590_01930"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG23389.1"
                     /translation="MVASLEERQVYGEMMAGARQKVLITGVGGMLGTDLAEILKNDFE
                     IIGLDKKPAPHLGRPIEICDLLQERKLRDVMLAHRPSVLIHCAALTDVDYCEEHPEEA
                     ETQNVEATRNLVVLANELDIPLVFFSTDYVFGGTREGEYLEDDTGKPLSVYGRTKWEA
                     EEVIRSTAKRAALCRISWLYGVYGRSFPRAILGQTESVKNIRVVSDQTGRPTYTKDVA
                     GAILRMLSEKKETVF"
BASE COUNT         2947 a         2744 c         3370 g         3079 t
ORIGIN      
        1 ccgcgtaaaa aacaggggtt gagccttgac gatttcttcc gtgattccct gaagggtgtt
       61 ctttctaagt tccagcgcct taatgaaatc tattccgccc gcgagttttt ctttgaggaa
      121 acgcgcggtt ttttcgtcca gatcccggcc tcggagcatt tttcgatatt cctgattcag
      181 cgtgagccgg gggatgaatt cctcgttcat ccggatttcg agacctccgg tggttccttc
      241 tttggtggag acggtgacgt cgggcgtgac cgcgatgggt tcttccgcgt aaaagcttct
      301 tcccggcttg ggttcgagac gggagatcat gtcgatggca ttttgaatcg cgcgctcgtc
      361 cttatgaaaa atcttggcga gcgacttata atctttccgc gcgaccagct ccaggtgatg
      421 aagaaccatc tctttggcaa gtgccgcccc cggtcctttc cgctggagct ggaggaagag
      481 agcctctttg aggtcccggg cggccactcc ggggggatct agctcctgga tttttgtgag
      541 aacaggcccg atcgcggcgg gatcagtttg ggcggccttg gcaatttctt cggtggttgc
      601 cgccagataa ccgtcctctg tgatgttccc gataatctgc ttggcgatcc tttgctcggt
      661 cccggaaagg tccaggaaac gaagttgcca ttcgagataa tcgaaaagac tttccggagg
      721 agtcaggagg ctgtcctgaa aatcctttag tttttggctg acgctcgcgg ggcgttttga
      781 aaaatcaacg gacgggcgga gaaaatccag cggttcggcc ccttcccaat ctccggaaat
      841 gacgggggag ggatcgtttt cctcgtcgtg tggttcttgg ggactttccg cggttgcgag
      901 ctcgggatca agcggcaagt tcgcttcttc cagcatggga ttttcttcca ggacggcatg
      961 gacggcctgg cgcagatccg cgaccgggag acgcagaagg cgaaggtatt gccgcatttg
     1021 cggtgagagc aaaagctttt gttgttggaa ttgctggaga ttttgtttca tgcggcaaaa
     1081 taccatgacc gtaaaatgtt gtaaagccat gagatttctg aagttacaaa agagaaaccg
     1141 attgacactc acgtcttatt ttttataatg aacacgttcg tgtttcggga ttcaccacga
     1201 gacagagctg aagttttctc tcgtggtttt tttatggtga cagaatagac catgacttct
     1261 aaaaaggaac cgacaatgtc ccaagcgcct gccctgatga ttacccgggg gctgagaaaa
     1321 aaaagcatgc ccgaaggtct ttggaccaaa tgccccagtt gtcaaaccgt tgtttttaat
     1381 aaaactctca aagaaaactt gctggtttgc gcgaagtgta actaccattt cattatggga
     1441 gcctgggagc ggatctatca tttggtggat gacaagacat ttcgtgaaat tgccgcgaat
     1501 ttgcagccag gggaccctct caagttttcg gccgtcaagt cttattctga aaaagtaaag
     1561 gcggatcaaa aacgcaccgg gctgaaagaa gcctgtatta tcggggaagg caagatgaat
     1621 ggtcatccgg tcgcgctcgg cgtgacggac tcgcgtttca tcatgggttc catggggtcg
     1681 gtcgtgggcg agaaagtcac gaagttaacc gagtatgccc ttgagaataa actccctttg
     1741 attattgttt ccggttccgg cggcggtgcc cgtatgcatg aaggcgcgct cagccttatg
     1801 cagatggcca aaacctcggt ggctttgacc cgttaccaga aggaacggtt tccgtttatt
     1861 tcgattctga caaatccgac catgggcggc gtgatggcaa gttttgcgtc ccttggtgac
     1921 attattatcg cggagccccg cgccttgctc gggttcgccg ggccccgggt cattgaacaa
     1981 acgatccggc aaaaactccc gcctgatttt caaacttccg aatttttcgt cgaacacggg
     2041 atggtggata tggtgattga ccgcagggac cttaagcaaa aaattacgac gattctcgaa
     2101 atcctgtgct aactcgggga ttttttaatt atggccaacg tagctcttaa aaacgtttcg
     2161 aagatttata aaggggatgt caaggctgtc aacggagtga atattgatat ccaggaccgg
     2221 gagtttgttg tgcttgtcgg gccgtcgggc tgtggcaaat cgactacgct tcggatgatc
     2281 gcggggcttg aggaaatttc ggagggagaa atctttatcg aaaacacgcg ggtcaacgat
     2341 gtccccgcga aaaaccgcga catcgccatg gtttttcaaa attacgcact ttatccccac
     2401 atgacggtct atgaaaacct ggccttcggt ttgaaactcc gccggtatcc ccgtcgggag
     2461 attgaacgtc gggtacatga agccgcggaa atcctcgggc tttcgttgga tatgcttcag
     2521 cggcgcccga aggaactttc gggcggcgaa aggcaaagag tggcggttgg tcgcgcgatt
     2581 gtccggaaac ccaaggtttt tctttttgac gaacctctga gtaatctcga tgccaagtta
     2641 cgcggacaga tgcgcatgga attgcagaaa ctgcacaacc ggcttcagtc gaccatgatc
     2701 tatgtgactc acgaccaaat cgaggcgttg acgcttggag atcgggttgt ggtgatgaac
     2761 aaaggggttg tccagcagat cgcggatcct atcaaccttt atgagcgacc gataaaccgt
     2821 tttgttgggg gatttattgg ttccccgccg atgaattttc ttaaaggaac gattgccgcg
     2881 gagaatggca attacttttt ttccgacaaa agtttaaaac tgcgtattct tccacactat
     2941 tatccaagga tctctgctta caaaaatcaa ccggtgctga tggggattcg gccggaagat
     3001 atttatgaca aaatttttgc ccaggacgcg cgaccggagt ttacgaccac ggcgaccgtg
     3061 gatgttgtgg agccgatggg agccgagatt tttttgtacc tgacagcctg cagtaacagt
     3121 tttgtggtac gggtttccaa tcaggatacg gcgaccatga atcaagatct tcaggttgtt
     3181 tttgatatga gtaaagcgca ttttttcgat cctcaaacag gtgtagtgat attttgagac
     3241 cttaaaaatc atgggtgtgc cagcccgacg tcatctagtg tttttggctg gagtgggggt
     3301 ttttgctttt tcggttttct tgatatttca gtcccttgcc cagcttcgta cgaccctcca
     3361 tcccgaagtt tatcttcagc gattgtcgtt gattttcgcg gctctcatgt ttccgatcct
     3421 cacgacctgg tggcgcttta aatggattgg cgtcaatatc ctgatgattt ttttggtgat
     3481 cccgctggtc ttatttgtgg tttcggtgac gaagatatcg gtgtttttct gggtttatct
     3541 tgcctatgcg atcctgatgc tggttcttta ccgcaccgaa catcagtttg aaaaccagct
     3601 ttccatgatt ttgatcgagg aagagaaact gcaagatgag atcaataatc tcgaggtcgt
     3661 tttcaaggcc aaaggggagg ggatcagtac tttttttgaa aagtattcga cctactataa
     3721 cctccggaaa ctcgccgaag aactcacgat gagcctttcc tggaccgagc tcgcgcaaaa
     3781 aacggtggat cgggcgatgg attttattcc ccggggcgac tacgcgaaaa tgacgttggc
     3841 gagaagtgat gaaagaaatc ttcccgtggt ggccagcaga cgtatgcgcc cggtcgggcg
     3901 gtggacggcg aagcaggggg atctttttga tttctggtgc atcaaaaatc gcaagcgtct
     3961 aattgtcatg gattcgcagg atgattttcg cttcgcgctc cgcgaaaacg acaaaagaga
     4021 aaatttgcgg agtttgatcg tcgcgccgat tttttttgaa aacagagttt caggaacgct
     4081 cgcgattcac gccacccgtc ccaacgtgtt tacgcatgaa gacctccggt tgctggacgc
     4141 gattgcgacc ctcgcttcct cggccatctc caacgccatg ctgtatgaga agacggaaca
     4201 attggcgatc aaagattcgc tgactggact ttttgtccgg aggtatttct acgagaggct
     4261 caaggaagag catcgccgcg cgcttttaaa tcagaaaacc atgtcgctaa tcatgtgtga
     4321 cctggatcac ttcaaggaat gcaatgaccg gtttggccat cagggcggcg atttgatgct
     4381 ggttcagttg gccgaaatcc tcggacagtc tctgaagaat gcgttactcg ctcgctacgg
     4441 aggtgaggaa ttcgcgattt tacttcctga aacgacaaaa gcggaggcct tcaagattgc
     4501 tgagggtttg aggaaaactg tagaaaccac cccgtttcac attcgccgcg aaaaagttgg
     4561 aatgacggta tcagccggcg tctcaaacct tccggatgat acgctggata tcgaactgct
     4621 ggtccataag gcggatcagg cgctctacag agccaaacgt gaggggagga acaaggtatg
     4681 cgtaagcacg tcttgatcct tttgtttcaa cttggcttgg cggcgatcat tcttcatcag
     4741 acccgggaac tgattctcgt gtattatctt ttggcgcccg ttttgctgct tccggctttt
     4801 tatttttaca aggagcggtc gcgcgggatg ttattggtgg cgctactcgt ggcggctgct
     4861 gtgaatctgc gttacttttt tctggatccg atgccccgac tttttctttt ttggggaggg
     4921 caagcagttt tttttatgct ttcatgcgct tattcccggg agctgaggat tgctttggat
     4981 tacgcctcaa gcaaaaaaaa cgaggtccgg aaaaatctgg aaaatttgcg ggcgaaattt
     5041 caaacccgga tttccagcct taagcacctc gaggcgcagg tggggagcct gatgaacctt
     5101 tttgagatcg cccgggattt tagcgagtgc atggattttg ccactttgtc ggattttctc
     5161 cttaaaaaac tgcgaagtga actttcgttt caacggatgc ggatttttat tttaccgctg
     5221 acgcccgcgt ccagggagaa aggaggcgtc aagctttacg agatgaccct ggacggtgtt
     5281 caggaaatcg agcgggagtt aagtgatgag gaaaaaaccg attttgattc aatggcgctc
     5341 accaaaaaca tggtgagacg tgaaaaaatg tggtttttcc ctattttgga ggccgagaag
     5401 ctgaccgctg tattttcgat tgacggtgtt caggatgaag atctggcccg tgtggaggtt
     5461 ttgagcgcgt atctggtcct tttgatcaag aaaatccagc tttatgaaac cgtgcgtgag
     5521 cttgcgatta ttgatgagtt gacccaggtt ttcgtgcgtc atcactttgt tgaacggctt
     5581 gaggaagaag ttcgaaggag catccggttt aaactgcctc tgacggtatt aatgttagac
     5641 atcgatcatt tcaaaagata taatgacgaa ttcggacatc tcgtcggcga cgcaaccctg
     5701 aaagaagtcg cggaccttct caaaaaaaat cttcgtcggg tggatcttgt gggccggtac
     5761 ggaggggaag agtttgtggt tgctatgccg gagacgcgta tggaaaacgc gtgcgaggtc
     5821 gctgaacgta tccgctccaa tattgcgagg cacgacttcc gggtttacaa tgaccgaacc
     5881 aaggtgacgg tgagtcaggg cctccgggtt tttgacgcgg cttcgatcag ggagcccgaa
     5941 aaggtagatg tgccgaagct ggttttagaa ctgatacacc atgccgatga atcgatgtat
     6001 cgcgctaaag aagaaggacg taaccgggtc tgtgtttaca aggaacccca taatctgtta
     6061 tgagccaatg tttttttaaa aggatcgcat tttttagtac gcttctgctt gccctcacgg
     6121 cgttgattcc cgtctcattt tcccaggatg ataccccgac aaccccttcc gaaaaacagc
     6181 ccgtgacggc cccccgaaac ttgccttatc cgacggtgcg ggatcaatcc cgggctgaag
     6241 ttgaggcggt cgcggacacc ctcgagtatc aaaaagattc gcggaagctg gtggcgcgcg
     6301 gaaatgcggt cgtgacccac cagaatatca agcttttggc cgattacgcg gaagtggaga
     6361 cggaaaccaa aaaatcattt gccaagggac acgtaattat ttttgaaaat gaaacaccga
     6421 tccttactgg tgatgaagtg tactatgatt tttcaactcg ccaggccagc atgccaggcg
     6481 cgaaagcaat tcaaacgccc tggtacgcgc ggggcgagga agtccagcaa gttaaacagg
     6541 gtgaaattca cgtcaggcac ggcggcgtga cgacttgcaa tcttgaaaat cctcactatg
     6601 aaatccggtg taaaaaggca accctctaca gcggggtcaa gatccgcatg gtcaacgtca
     6661 ctattcacgt tctcggtaaa ccggtattct ggttgccgta tcttgaagtt ccgcttaact
     6721 ggaatattcc atttcaggtc cgcgttggta gcagtgacca gtatggcacc tacatcgaac
     6781 tttcaaaggg aataacgttc aacaagtacc tgtccggaaa atggcacgcg gattggcggt
     6841 cgaagcgcgg atttgggggt ggatgggatc agaactatga ttttggtaag tacgcgaagg
     6901 gggatgtcaa actctactgg acccaagata agcgggcgcc gacccccaat cattacgatg
     6961 ccgatggcaa attgaatccc tatgaattgc gtgaagatag agatcgtgga cgaattacct
     7021 ggcgccatcg gaccgatttt tcggaataca cgaacattat catgcgttat catcgagtcg
     7081 ctgacggata ttttttgaag gattttgccg aaaatgagta tcgtgccgag atggagccgc
     7141 attcctttgt gaccgccact cataacacgc aacggtgggg cgcgatggtc cacgcggaga
     7201 aaaaaatgaa ttcccacgaa aacatcattg agcgccttcc gcaggtccga ctcgactgga
     7261 aaaatcagcc cctcatgggc gacaaggttt ataacgtcag ccggttgcag tttgataact
     7321 tgggcaaaaa aatcggttac agcgattaca acgaagatgt tattcgggtg gacggttaca
     7381 gccggttgat cgcgccagtc aagctgggcg atgtaaaaat gactccttat ctgggttacc
     7441 gcgcgacgga atacagtcgt gatctccgga cggacggggc ccatttcagg caagtgcttg
     7501 aatacggtct tgatgtcaga acacaggctt acaaaacgtt tgatgtcacc tttgagaagg
     7561 ccgggatcga agtgaaccaa ttgcggcata tttttgaacc gaatgtgagg gtccaaggaa
     7621 cggattccac attgagttac gagaaaatag cgcatttcga cacggtagac atgattgacg
     7681 acgcgtctga aattgttttt gggatggata accggcttca gaccaaacgc gtcgcaaacg
     7741 ggcgaaacca gcgggtggat atcgtcagca tgaacacgta cgctcatttc gcgatttctc
     7801 cgcaggaccc taacattcaa ggcgccaagt ttacgttggt cgaaaatgag ttgaccctca
     7861 ggccgtatga ctggcttcag tttcagcatc gcctggaata tgacgccgcg cgaaattaca
     7921 ttaaacggtg caataacgat attgtgatcc gtcaccagcg tcttaaactc acgtttggcc
     7981 accgattcat tcatcggcag tatgactata cgcaaaatct cgacattgat acttcccaac
     8041 aatttgtgtt tgacggagaa tataagctca atgacctgtg gactttggcc ggttacattc
     8101 gatgggacca aaaagggagt tcgttgcagg aatggcaggt gtcggcaagc cgggatcttc
     8161 acgattttat tctggatttc ggttataacg tccgggattc cctgatcaat tcaaagaaca
     8221 atgagctttt ttttaacttc cggatgaaag attttcccgg catggacttt gggctgggcg
     8281 gggggcgggc gacgttttca cccccacgca ttggtgagac cgtcgaaggg gcaagtcagg
     8341 atcgatccct tctttccaag agtttttaat taacccttca tttctgttta atctcacaac
     8401 gggaacttaa ggagtattct tctgcgatga aactggcagt gatcgggtca gggtatgtcg
     8461 ggttggtgac cggggcgtgt ttctcgaacc ttggccatga tgtggtttgc gtcgataacg
     8521 accctgaaaa aatacgaacc cttaaggcgg gaaaaatctt tttttatgag cccggtcttc
     8581 atgagatggt gtcggaaacc gtggccgcgg ggcgtttgtc ttttacgacc gaaatcaaaa
     8641 aagtagtgaa ccgttgcgaa gttatattta tctgcgtcaa tacgccgtca cttccggatg
     8701 gaggggcgga cctgagctcc gtggaaaagg tggcaaaggt tctcgcgaaa aacctgcgca
     8761 agtattgttt gatcgtcgcg aaaagcacgg tccccgtcca gaccggagag tggatccgca
     8821 agacggtgaa acggcacgta aggcccggcg ttaccttcga tatcgcgtcg aatcccgagt
     8881 tcctgcggga aggttccgcg attcatgatt ttttgaaacc ggatcgtgtt gttgtcggga
     8941 ttgactcacg ccgggccgag aagatctttc gggatcttta cgcgagcatg aaagcaccct
     9001 tgatcgtcac ggacatcaaa agcgccgagc tgatcaaaca cgcctccaac agttttttgg
     9061 ccgccaaaat ctcctttgcg aacggtttgt ccagaatttg tgacatcgtg ggggccgacg
     9121 tggaccaggt gactttggga atggggctgg atccccggat tgggccgcag tttctcaagg
     9181 cgggagttgg tttcggaggg gcgtgttttc cgaaagacct gaaagcgttc cttcatattt
     9241 ccgacaagct gggcgccagg tttgatcttt tgcaggaagt tatccgaatt aacgaaagcc
     9301 aggccatgta ttttgtccgt aaaattgtaa aagaaatgaa gtctctcaaa gaaaaaacaa
     9361 tcggggtgct ggggctggcc tttaaatcgg ataccgatga tatgcggaac gctccatccc
     9421 tcgcggtgat tccggaactt caaaaagaag gagcccgcat acaggcctat gatccccagg
     9481 cgatggagaa ggcacgcccg catttaaaac gggttcaata cgtcgccgga ctttatgagg
     9541 ccgtcaaggg agcggacgcg gttgtgttac tgacggaatg gaaaaaattc cagaatatgg
     9601 attgccggaa ggcccgaaag ttgatgaagg gaaagtattt cttcgacgga cgtaacatgt
     9661 tcgagccttc tgaaatgaag cgttatggtt ttcggtacgt gagtatcggg cgaccatcga
     9721 agtgaacgtg aattttcgga acttcatttt ccggtagacc gggaggggag gttggccaat
     9781 gaagggtatc attcttgccg gaggattggg aacgcggctt tatccgttga ccaaggtcac
     9841 gaacaagcac ttgctgcccg tctacgacag gcccatgatc tactacccga ttgaaactct
     9901 ggtcaaatcc gggatccggg acattttgat cgtaaccgga ggaaacaatg ccggcgattt
     9961 cctgaaactg atcggtaatg gcaagcagtt cggcttgaag cacatcaatt acacctacca
    10021 ggagggagag ggcgggatcg cgcacgcgct gagcctcgcg gaacatttcg cggacgggaa
    10081 aaaaatagtc gttattctcg gggacaatat ttgtgaagcg aatatctcat cgtacgttca
    10141 gagttttaga aaacaaccgg acggcgcgcg gatccttctg aaaaaagttc cggacccgga
    10201 gcggtttggt gtcgcgacga tccggggctc ccggattacc aaaattgtcg aaaaacccaa
    10261 aaaaccggaa agcgcttatg ccgtgaccgg aatttatatg tacgataaga gtgtttttga
    10321 gtttatcaag actctaaagc cctcacgccg gggtgagctc gagatcacgg atgtcaacaa
    10381 ctggtatctt aagcgaggcg ccatggagtt tgatgttttg cccggttact ggacggacgc
    10441 gggaaccttt gagtcgcttt ttcgggcgaa cgcgcttgtg gcgaggcaca agggcgtggg
    10501 acgatcatga aaaaaatttt ggtgacgggt gggtgtggat ttatcggttc caattttatc
    10561 aggtttttcc tgcgtgagcg accccgttgg gccgttatca atctcgacaa gatcagctat
    10621 tcgggaaatc cgaaaaatct tcaagaccta aaaaaacatc cccgctatga gtttgtccgg
    10681 ggcgatattt gtgacaagaa acgggtggct tcggtgatgg cgaaagcttc ggcggttgtt
    10741 catctcgcgg ccgaaacgca cgtggatcgg tccattgatt cagcggatga gtttattcgc
    10801 acgaatgttt tgggaagtac ggttttgctt gaggcttcac tcgctcgcgg gattgaacgc
    10861 tatgttcata tgtccacgga cgaagtttac gggagtgttc ccagaggcaa atcaaatgag
    10921 ttgtcgcctc ttgcgccgaa cagtccctac gccgcttcca aagcggccgc ggatttgctg
    10981 gtgagatctt ttcaggtcac tcacaagcat cccgccatga tcgttcgttg ttcgaataat
    11041 tacgggcctt atcagtttcc cgaaaaggtt attccgcttt ttgtgacgaa tttgcttgag
    11101 gacaagccgg tgcctctcta cggggagggg aagaattgcc gggagtggat ttttgtcgaa
    11161 gatgcctgcc gggccatttt gacggtgctc gaaaaaggaa aacccggtgg gatttataat
    11221 gtggggtcgg ggcaggaacg atcaaacctc cagttgaccc gggccattct gaaacagatg
    11281 gaaaaaaagg attcgatgat acgtagggtc caggaccgtc ccggacacga ttttcgttac
    11341 gcgcttgatt ttggaaaaat ccggaaactc ggatttaaac cgtggtggaa ttttgaacgg
    11401 ggattgaaaa caacggtgga ttggtacaag gttaacgaag gatggtggcg tcccttgaag
    11461 aaagacaggt ttacggtgaa atgatggccg gcgcgagaca gaaggtgctg attaccggag
    11521 tcggtggaat gctcggaacc gatctggctg aaattctgaa aaatgatttt gaaatcatcg
    11581 gcctggacaa gaagcccgcg cctcacctgg ggcgcccgat cgagatttgc gacctccttc
    11641 aggagcgcaa actaagagat gttatgctgg cccatcggcc ttcggttttg atccattgtg
    11701 ccgctttgac ggatgttgat tattgtgaag agcatccgga ggaagccgag acgcaaaacg
    11761 ttgaggcgac ccgcaatctt gtcgtattgg ctaacgaact ggatattccc ctcgtatttt
    11821 ttagtacgga ctatgtcttt ggcggtaccc gggaagggga ataccttgag gatgatacgg
    11881 ggaagccgct cagtgtttac gggcgcacca aatgggaggc ggaagaggtg attcgttcaa
    11941 cggcgaagcg ggccgcgctg tgccgaatca gctggcttta tggggtttat ggccgttcgt
    12001 ttccaagagc gatcctgggc cagactgaat cagtcaagaa tatcagggtc gtgtcggacc
    12061 agacgggacg accgacttac acgaaggacg tggccggcgc tattctgcgg atgttgagtg
    12121 aaaaaaaaga gacggtattt
//
LOCUS       DAOSIO010000027        12121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_5277_length_12121_cov_4.700232, whole genome shotgun sequence.
ACCESSION   DAOSIO010000027 DAOSIO010000000
VERSION     DAOSIO010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 12121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12121
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5277_length_12121_cov_4.700232"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PK590_01935"
     CDS             complement(<1..568)
                     /locus_tag="PK590_01935"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOG23390.1"
                     /translation="MKKTKLKIMVFVVTALCAVSGMALAGAPDASQKIGVAAGITGEV
                     RSVSLSGEERALGSGDPVYAGDTISTGANSKMQILLLDQTIFTIGASSGIKLDEFVYD
                     PATSEGKVNADIIQGAFRFISGKIAHRKPENMSVDLPAGVIGIRGTCVAGQSEGDRSL
                     VVLLGDSSGQNSGAINVSNNVNGQSVGVD"
     gene            complement(606..2036)
                     /locus_tag="PK590_01940"
     CDS             complement(606..2036)
                     /locus_tag="PK590_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG23391.1"
                     /translation="MRIKGNLFARLFFCFFILTFGVVSPALAEDLAQEVRAVRREVWS
                     ERYADAVAQKSGALEESKKFKTGVTFDDILKDPDNLELNFQFAQQQIANNELIGAAAT
                     LERILLVKPNLADVRLLYGVVLYRLNSLDEAKRELDSLQRLQLPRDIEAQVNEYRKRI
                     DARKRRTRFGLRETIGWGYDTNRNAAPSSKFQLAGEVPVGLEGTNRKRDDTHFTNITT
                     VDFRHDLGMPAGHELFGSFTYFLQEQTKVDSLDIGMFQYELGGRYKSKWINVAPSFVA
                     SNILLSRETYLRTLGLNILMDRQITKKVSGFMNFRYDYQRYSGISENTTGAQRKGPEY
                     DYLWGISYAPVSTMRIIPSIGYTNKNAKSRFDGYDRFNLNLTHTWALWRGTFLINTAN
                     LYFDRYDEPETAIAGRFRHDKIFRYRVTAGAPLSALGLGKVMPKMMRDIVFTLSYEYY
                     RSLSNITNYAYTNNKIEGLFTKRWEF"
     gene            2268..4226
                     /locus_tag="PK590_01945"
     CDS             2268..4226
                     /locus_tag="PK590_01945"
                     /inference="COORDINATES: protein motif:HMM:NF019758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG23392.1"
                     /translation="MEHTDFVLDPFQQQAIEWIEKDHSLLVSAPTGSGKTLIAERAVE
                     KAISRGKNVIYTAPIKALSNQKFRDFQKRFGPDQVGILTGDVAINPGAPLLIMTTEIY
                     RNSLFENSERIKNTGWVIFDEVHYMDDYERGTVWEEAILFTPPEIRILALSATIPNVR
                     ELASWMQTAHDRPIAVIEETRRPVPLHFFYQCQGKFFTNLKSLRKEGYYSRDNWELSP
                     RERRRGFRQMRAKPNRLNELLDHLIREDRLPAIYFAFSRKRTAYLAWEIAHYELLDKH
                     ERVKVTEIFDKLCEKYDLTEERSAQDIRPLVEQGIAYHHAGMLPTLKEVIERLFTSRL
                     IKLIFTTETFALGINMPARTVIFDELEKFYGTGFKTLTTRDFYQMAGRAGRRGMDPEG
                     FVYCRILPHDIPFPQVERVIFGKPEPVRSQFNAAYATLLNLYRDLGEDLLEIYPKSFH
                     YFQSSRRMRDNGFDLIQNKLSLLKELNYIQDGKLTEKGEFASSIFGYELLLTEMYQDK
                     VFDEFDETRLNVFLMGLIFEPRKGVEEPPKLSPQNEKLLKLIQHYHRIIHKKEQVYRI
                     RPYTKPPYFHLAKAVESWTRGEPFDRVTRHSPEDEGSLVRYFRMVIQLLREITHAPHA
                     SDDVRKKAASARKLIDRDVVDAEKQLRA"
     gene            complement(4329..7466)
                     /locus_tag="PK590_01950"
     CDS             complement(4329..7466)
                     /locus_tag="PK590_01950"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOG23393.1"
                     /translation="MTKTLLIGPAGFGKTHAVLDSFEKSLRDGDPLTASSFFVVPSAE
                     HTERVVSMLIQRGLPGFFHKRVTTLSRLVDSVFQIPEIPVASGMTRTLLVRELVSEND
                     WEYFREVKDRPGFVNLALGLITELKESLITPEAFRERMNSLKNFEPAFGAKYEALAAI
                     YEGYEVKLKERGMRDSQDTIRIFRERSEKKSGEVRFRDLWLDGFFDFSNLQFEYLREL
                     SSMTERMTVTLTVEEGEAFEMVQSTRRALEGIGFTVVKTGTRNFRTKNPALIHLRENL
                     FREPRGNSARSVCSSRGVIFLESVGVEGEIELIAREIHKIYSRGNCRYSDFAVLFRQV
                     RHYGPMIASVFSRYGIPVEIHERERLKFSPWIGAVISLLLIFRNGWHRDDVLAFLKSG
                     YVTRIGEMPKTEEWLSRFEAGVFMNGVLEGREMWEKDWAGEESLVVNSESGKKKVLET
                     LIRLESDFLKAQSVEEHIRILKQAVTRTFGMIETSDAYTPMVRRDAVSVNRFETLLEE
                     IRLNETRGRGRPVSFEFFADQFLGLVELDVYSLHERDKNRVQIYDVSLARQKEYKTVF
                     VAGLLERVFPIQIRENALLSDWERRMINAGLEHPLQELLPRQSLERYLFYLAVTRASE
                     RLYLSCPRVNLEGKESLKSFFLEDVREIFGSGDSVQVIRQDLSRPYPSVDESITGRER
                     EIAVMGVAREMSEKKRGKGKLDGAAPVSLEKLLDALRPVKASLEDEAIRAGGYFEIRE
                     VSPTRLEEFARCPYRYFAHQILKLQDPMEDMTARQKGNIMHWVLEVYFREWIRQNGKR
                     PLPAEFVETKLAEAFEKYPLSSERKYREELDRAEVREMLFSVIEKEAETLLSRKFQPA
                     YVEFGFGPGKERDAPAFVIVPGKKGIAIRGRIDRIDIDPERKAALVVDYKRTAKLDRS
                     ALELGTSLQLPLYILVAEKLLGLTLVGGELFALKEGKRSGFYQKDECEKYGLQVSKLS
                     GMSGEEFRRALDRSVDFVKRFTEEIRGLNILVRPRDCEKYCPYAALCRIEKWRIPVIL
                     QEIREADARAVGQGS"
     gene            complement(7590..8021)
                     /locus_tag="PK590_01955"
     CDS             complement(7590..8021)
                     /locus_tag="PK590_01955"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOG23394.1"
                     /translation="MYQVAKTLYFNYGHRLLGGHDKCSHLHGHSAKVRIVLRARKLDR
                     RGMVVDFFDIKNTIGHWIERELDHKMILNARDPLVKLLQKAGEPVVTVQGNPTAERLA
                     EWIFKESRRMKLPVVEVSLWESEDSMAVYTANLKSRERNEP"
     gene            complement(8009..9202)
                     /locus_tag="PK590_01960"
     CDS             complement(8009..9202)
                     /locus_tag="PK590_01960"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOG23395.1"
                     /translation="MDSREKPHGDVPRGKNNVRPLLEKQDSSPLIEKIRANDYCLRSG
                     RLEIYLAREFGFCYGVERAVEYAYETRLKFPDRRIFLTDEIIHNPRVNRELRELGIRF
                     LSGPSACGLAVTDLDEKDVVIMPAFGVPVNALKEYQERGCVIVDTTCGSVMSVWRRVE
                     SYARDGFTSVIHGKYDHEETRATCSRAGKYLVVRDQAQARQVCDLILRGGDKASNTPR
                     ENDLFLADFKRVCSEGFDPGKDLEKIGFANQTTMLMSESIVISGMLREGLEARYGNEA
                     ARARFRHFDTICSATQDRQDAIRALGEKHMDLILVVGGYKSSNTGHLAEIASRYAPTF
                     HIEDADGILSKEKISHRNIDTQMITETDGWLPGGTAKVGITAGASTPNHVIEEVIERL
                     MECTR"
     gene            complement(9209..10207)
                     /locus_tag="PK590_01965"
     CDS             complement(9209..10207)
                     /locus_tag="PK590_01965"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HOG23396.1"
                     /translation="MIIGLTGKNGSGKGEAANFLKERGFHYYSLSDALREEAGKRGQP
                     LARETLVALGNELREKEGPGCLAERIYAKLDPEKHYIIDSIRHPAEVQVFRRRADFKF
                     LRVNAPERLRFERMKQRGRENDPETWEDFQALEAKESKSETKTDQQLDQTLALADLQV
                     DNNGPLKELHDKLRQLLVKIAQSSTRPGWDEYFMNIAKVVALRSNCMKRKVAAVIVKE
                     KRIISTGYNGTPRGITNCNEGGCPRCASVETSGKDLGECICSHGEENAIVQAAYHGVS
                     VKGATIYTTFSPCLMCTKMIINSGIQEVVYQATYPLSDTSFKLLQQAGVAIRQLSV"
     gene            complement(10440..11027)
                     /locus_tag="PK590_01970"
     CDS             complement(10440..11027)
                     /locus_tag="PK590_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23397.1"
                     /translation="MPHTVAQLNIMALEKILKKHIGDFASENTAARVVFRGLEVMGVG
                     LWPVLDHFLFRSANPEERAREFTELGYTRDISSRILGRPRDPVHVYRCRCLPAILIEE
                     TRAPESKEWGKVFGENAPYVMGIRVADIDEATFHLEKQAVRFLRPGAGKPGEDIRAIA
                     AIPAFQDGKEKSVLLLIERHVTGVNYFAPGFWNKA"
     gene            complement(11131..11442)
                     /locus_tag="PK590_01975"
     CDS             complement(11131..11442)
                     /locus_tag="PK590_01975"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG23398.1"
                     /translation="MAYVITEKCHGERYGLCATVCPTEAIYPGTYQGKDFMVIDPTVC
                     ISCGACAAECPVGSIVESEDMDPTWAKINADLAVEFKSNPKVAPRARNESPKKPTNQL
                     K"
     gene            complement(11588..>12121)
                     /locus_tag="PK590_01980"
     CDS             complement(11588..>12121)
                     /locus_tag="PK590_01980"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOG23399.1"
                     /translation="ELSHIMSHDILITTIVAAITGLPGMIAAMLPRSADWGSGERYER
                     GETHPVVFFLTLLWIPVAVVLVRGFIPRRREYKADRQGGTWCGNPLYLASALRKLGPE
                     SEKFPLRSAEPATAHLFIVYPLSGQRWTSFFRTHPPVEARIARLEAQYRDSRRSDERL
                     CSSQPPHLWPEGATDCP"
BASE COUNT         2971 a         3487 c         2831 g         2832 t
ORIGIN      
        1 tgtcgactcc gaccgattgc ccattcacat tattgctgac gttaatcgcg ccggaattct
       61 gtcccgaaga atcgccaaga agcaccacaa gggaacgatc tccctcgctc tgacccgcca
      121 cacaggtacc ccggattccg atcactccgg cgggaagatc gaccgacatg ttttccggtt
      181 ttcggtgagc gatctttccg gaaataaatc gaaaagcgcc ctgaataata tcagcgttca
      241 ccttgccctc ggaggtcgcg ggatcataca caaattcatc cagcttgatc ccagaagaag
      301 cgccgatcgt aaaaatcgtc tgatccagaa gtaaaatctg cattttggaa ttcgcgccgg
      361 tagaaatcgt atcgcccgcg taaacagggt ctccgcttcc cagcgcgcgt tcttcacccg
      421 aaagcgacac gcttcggacc tccccggtga ttcccgccgc gaccccgatt ttttgtgagg
      481 cgtccggcgc tccagcgaga gccattccgg aaaccgcgca aagcgccgtg acgacaaaaa
      541 ccatgatttt caattttgtt ttcttcatct gttttctccc ctcacgtgac cgtgatgccc
      601 cgggcctaaa actcccagcg ttttgtgaaa agtccttcga tcttgttgtt agtgtaggca
      661 taattcgtaa tattggaaag cgaccggtaa tattcgtagg aaagcgtgaa cacaatgtct
      721 cgcatcattt tgggcatcac cttaccaagc cctagggccg aaagcggcgc gccggccgta
      781 acccgatagc gaaaaatctt gtcgtgacgg aaacgtcccg cgatcgcggt ttcgggttca
      841 tcgtagcggt caaaatagag atttgccgtg ttgatcagaa aggtcccccg ccaaagcgcc
      901 caggtatgcg tgagattaag attgaacctg tcatacccgt caaatcggga cttcgcgttc
      961 ttgttcgtgt atccgatgct cgggataatc cgcatcgtag aaaccggcgc gtagctaatg
     1021 ccccacaaat aatcgtactc cggccctttt cgctgggcac ccgtcgtatt ctcggaaatt
     1081 ccggaataac gctggtagtc gtaccgaaaa ttcatgaatc ccgaaacttt tttcgtgatc
     1141 tggcgatcca tcaggatatt gagccccagt gtcctcaggt aggtttcccg ggaaaggagg
     1201 atattacttg ccacaaaaga aggcgcgacg ttaatccatt tgctcttgta acgaccgcca
     1261 agctcatact ggaacatgcc aatgtccaga gaatccacct tcgtctgttc ctgcaaaaaa
     1321 taggtgaaag agccaaaaag ttcatggccc gccggcatgc cgaggtcatg tctgaaatcc
     1381 accgtagtga tattggtaaa atgagtatca tcccgtttcc gattcgtccc ctcaagtccg
     1441 acggggactt caccggccag ttgaaatttg gacgagggcg ccgcgttgcg gttggtgtcg
     1501 tatccccatc cgatcgtttc gcggagtcca aaacgggtac ggcgtttgcg ggcgtcgatc
     1561 cgtttccggt attcattgac ctgcgcctcg atgtcccggg gaagttgcaa tctctggagc
     1621 gagtcgagct cccttttggc ttcatccaga ctgttcagcc ggtaaagcac cactccataa
     1681 agaagccgca catcggccaa attgggcttc acgagcaaaa tccgttctag ggtcgcggcc
     1741 gccccaatca actcgttatt ggcgatctgt tgctgggcaa attgaaagtt aagctccaga
     1801 ttgtcagggt ctttcagaat atcatcgaag gtcacgcctg ttttgaactt tttagattct
     1861 tcaagcgctc ccgatttttg ggccaccgcg tccgcgtagc gttcagacca aacctcacgc
     1921 cgaaccgccc ggacttcctg cgccagatct tcggccaaag cgggcgaaac cactccaaaa
     1981 gttaaaataa aaaaacagaa aaacaggcgg gcaaacaagt ttcccttaat tcgcataaga
     2041 ttcgtctccc ggataaattt gaagtattga ataactttcg gactctctcg tcctgatcgg
     2101 cacagtttac cccgtatgtt tcataaaatc aaaaaatacc tttcatcacg acaaaatatt
     2161 tgggcgtctg gacagagacg ttaatctcga aagaccgact caataaaaaa caatagcggg
     2221 acaaatatcg acttggtcac aaaaaaaata aggttatact atgccgcatg gaacacaccg
     2281 attttgtcct cgatcctttt cagcaacagg cgatcgaatg gattgaaaaa gatcactccc
     2341 ttctggtttc cgctccgacg ggatcaggaa aaacgctcat cgcggaaaga gcggtcgaaa
     2401 aagcgatctc tcgcggcaaa aacgtcattt ataccgcccc catcaaggcc cttagcaatc
     2461 agaaattccg cgatttccag aagcgattcg ggcctgatca ggttggaatc ctcacgggcg
     2521 acgtcgcgat taatccggga gcgccgcttc tgatcatgac aactgaaatt taccggaact
     2581 ccctttttga aaatagcgag agaattaaaa ataccggctg ggtcatcttc gatgaagtcc
     2641 attacatgga tgactatgaa cgcgggacgg tctgggagga agcgatcctt ttcacccctc
     2701 ccgaaatccg gatcctcgcc ctctcggcca ccatcccgaa cgtccgggag cttgcttcct
     2761 ggatgcaaac ggcccacgac cggccaatcg cggtcattga agaaaccagg cgtcccgttc
     2821 cgctccattt tttttatcaa tgccagggaa aatttttcac caacctgaaa tccctccgca
     2881 aagaaggcta ctattcacgg gacaactggg agctctcgcc acgggaacga agacgcgggt
     2941 tccggcaaat gcgcgcgaaa cccaaccgtc tgaacgaact gctcgatcat ttgatccggg
     3001 aggatcgtct gcccgcgatc tatttcgctt tcagccgcaa acggacggcg tacctcgcgt
     3061 gggaaattgc ccactatgaa ctgcttgaca agcatgaacg cgtcaaagtt acggagattt
     3121 tcgacaaact ctgcgaaaag tatgacctca cggaggaacg ctccgcccaa gatatccggc
     3181 cgctcgtgga acaaggcatc gcctatcacc acgcgggcat gcttccgacg ctcaaggaag
     3241 tgatcgagcg gcttttcaca agccgcctga tcaaactgat tttcaccacc gaaaccttcg
     3301 ccctcgggat caacatgccg gcccggacgg tcatttttga tgagcttgaa aaattttacg
     3361 gcacgggatt caaaaccctg acgacaaggg atttttatca aatggcggga cgcgcgggac
     3421 gaaggggcat ggacccggaa ggattcgtct actgccggat cctgcctcac gacattccct
     3481 tcccccaggt tgaacgcgtc attttcggaa aacccgaacc cgtccgcagt cagttcaacg
     3541 ccgcctacgc gaccctgctt aatctctacc gggatctcgg agaagacctg ctcgaaattt
     3601 acccgaaatc gttccactat ttccagtcct cccgccggat gcgtgataac gggttcgacc
     3661 tgatccagaa caaactctct cttttaaaag aattgaatta tatccaggac ggaaaactca
     3721 cggaaaaagg ggaattcgcg tcctcgatct ttggctatga gctactactc acggaaatgt
     3781 accaggacaa ggtctttgac gaattcgatg aaacacggct taatgtattt ttgatgggat
     3841 tgatttttga gccccggaaa ggcgtggagg agccaccgaa actttcaccc cagaatgaga
     3901 agctcctcaa actcattcag cactatcacc ggatcatcca caaaaaagaa caggtctatc
     3961 gaattcgtcc gtacacaaaa cccccgtatt tccacctcgc caaggccgtg gaatcctgga
     4021 cacggggaga gcctttcgac cgggtcacgc gccattcccc ggaagacgaa ggaagcctcg
     4081 ttcgctattt tcggatggtc attcagcttt tgcgggaaat cacgcacgcg ccgcacgcct
     4141 cagatgacgt tcgaaaaaag gcggcgagcg ccagaaaatt aattgatcgt gacgtggtgg
     4201 acgcggaaaa acagctgaga gcttgaatcc tccggcttaa accacaaaac ccccgagaca
     4261 atctcccaag tatttttact gtttctcaaa aaaaatggaa aacgcgaagg ggatttgtaa
     4321 aaccaattct agctcccctg acccacagct cgcgcgtctg cctcccggat ttcctgcaat
     4381 atcacgggga tacgccattt ctcgatccgg caaagagcgg cataagggca atatttttca
     4441 caatcccgcg gccggacaag gatgttgagc ccccggattt cttcggtaaa acgcttcacg
     4501 aaatccacgc tccggtcaag cgcccggcga aattcttccc cgctcatccc cgacagttta
     4561 ctaacctgga gtccatattt ttcacactcg tccttttgat aaaacccgct ccgtttaccc
     4621 tctttcaggg caaaaagctc gccgcccacc agggtgagcc ccagcagttt ttcagccacc
     4681 agaatataaa gcggaagctg aagcgatgtg ccaagctcaa gcgccgagcg atccagcttc
     4741 gcggtccttt tgtaatccac cacgagggcc gcctttcgtt caggatcaat atcaatccgg
     4801 tcaatgcgtc cccggatcgc gatccccttt ttcccgggaa cgatcacaaa cgccggagca
     4861 tcgcgttcct tgccgggacc aaaaccgaac tcgacataag cgggctgaaa ttttcgggac
     4921 aaaagggttt ccgcctcttt ttcgatgacg gaaaaaagca tttccctgac ctcggcccga
     4981 tccagttcct cgcgatattt tctttccgaa gaaagcgggt atttttcaaa agcttcggcc
     5041 aattttgttt cgacaaattc agccggtagc ggtcgttttc cgttttgacg gatccattcc
     5101 cggaaataca cttcaagcac ccagtgcatg atatttccct tctgccgggc ggtcatgtcc
     5161 tccatcgggt cctgaagttt tagtatttga tgagcaaaat accggtacgg acacctggca
     5221 aactcctcaa gacgagtcgg gctcacctcc cgaatctcaa aatatcctcc cgcgcgaatc
     5281 gcctcatcct caagggaagc tttaaccggt cggagcgcgt cgagaagttt ttcaagagag
     5341 accggggcgg cgccatccag ttttcccttc ccccgttttt tttcactcat ttcccgcgcg
     5401 actcccatca cggcgatctc ccgttccctt ccggtaatcg attcatccac ggacggatac
     5461 ggccgtgaaa ggtcctgccg aatgacctga acggaatctc cgctcccaaa aatctcacgc
     5521 acatcttcca gaaaaaaaga cttgagggat tccttgcctt ccagattgac gcggggacat
     5581 gacaggtaaa gccgctcact cgcccgggtc accgcgagat aaaaaagata ccgctcaaga
     5641 ctctggcgcg gcaaaagttc ctgaagcgga tgctcaagcc cggcattgat catccttctc
     5701 tcccaatcgg aaagcagggc attttcgcga atctggatcg gaaacacccg ttcgagcaac
     5761 cccgccacga aaacggtttt atattccttt tgacgcgcaa gcgacacgtc ataaatctgg
     5821 acgcgatttt tgtcgcgctc atgaagggaa taaacatcca gctcgactaa tcccagaaat
     5881 tggtccgcga aaaactcgaa agaaaccggt cttccccgcc ccctggtttc attcagccgg
     5941 atctcttcga gaagcgtctc aaaccggttc acggaaaccg cgtcacgccg gaccatcggc
     6001 gtgtacgcgt ccgatgtctc gatcatcccg aacgtccggg tcaccgcctg tttaaggatc
     6061 cggatatgtt cctccacgct ttgagctttc aaaaaatccg attccagtcg gatcagggtc
     6121 tccagaacct tttttttgcc gctttcggaa ttcaccacaa gcgattcttc gccggcccag
     6181 tctttctccc acatctcgcg gccctcgagc acgccattca taaaaacacc ggcttcaaat
     6241 ctcgaaagcc attcttccgt tttcggcatt tcaccaatcc gagtcacgta accggatttc
     6301 aaaaacgcga gaacgtcgtc cctgtgccag ccgttcctga agatcagaag cagggaaata
     6361 acggctccga tccacggcga aaatttgagc ctctctcgct catgaatctc gaccggaatt
     6421 ccatacctcg aaaaaacaga agcgatcatc ggtccgtaat gtcgaacctg gcgaaacagc
     6481 acggcaaaat cactgtaccg gcagttcccc cgggaataga ttttgtggat ttcccgcgcg
     6541 atcagctcaa tttctccctc aaccccaacc gactcaagaa agatcacccc acgggaggaa
     6601 caaaccgatc tcgccgagtt gccccgcggc tccctgaaaa gattttcccg cagatggatc
     6661 agcgccggat ttttcgtccg gaaatttcgg gttcccgttt taacgacggt aaaaccgatc
     6721 ccctccagcg cccgccgggt cgactggacc atctcaaagg cctccccttc ttccaccgtc
     6781 agggtcacgg tcatacgctc cgtcatggac gaaagctccc ggagatattc aaactgaaga
     6841 tttgaaaaat caaaaaatcc gtcaagccag agatcccgga accggacttc cccggatttt
     6901 ttctctgaac gctcccggaa aatccttatc gtgtcctggg agtcccgcat gccgcgttct
     6961 ttcaatttga cttcatagcc ttcataaatc gcggccaggg cttcatactt cgcgccaaaa
     7021 gcgggctcga aatttttaag actgttcatc cgttcccgaa acgcctccgg cgttatcagg
     7081 gattccttga gctcggtgat aagccccagc gcaagattga cgaacccggg ccgatctttc
     7141 acttcccgaa aatactccca gtcattttca ctgaccagct cccgaacaag cagggtccgg
     7201 gtcatgcctg acgccacggg aatctccggt atctgaaaaa cggaatcgac taatcgcgaa
     7261 agagtggtaa cccgcttgtg aaaaaatccg ggtagtccgc gctggatcag catggatacc
     7321 acgcgctcag tatgctcggc ggaaggaacg acaaaaaaag aagaggcggt cagagggtcc
     7381 ccgtcccgca aacttttttc aaacgagtcc aggacggcat gcgtctttcc aaatccggcg
     7441 gggcctatta acaatgtttt tgtcatggga taaaaatgaa gtccggattt cgggtctcgg
     7501 aatccggaat acgcttaaaa agagtgaagg acgccaaatg acatttgtac tgttgggtct
     7561 ttttgacgcc ggaacaaggg atcattgttt cagggttcgt ttcgttcccg ggactttaaa
     7621 tttgccgtat acaccgccat actgtcctca ctttcccaaa gagaaacttc caccaccggg
     7681 agtttcatgc gccgcgattc tttaaaaatc cattcggcca gcctttcagc cgtcggattg
     7741 ccctggacag tcaccacggg ttctcccgct ttttgaagca atttgacgag aggatcccga
     7801 gcgttcagga tcatcttgtg atccagctcg cgctcaatcc agtgaccaat cgtgttcttg
     7861 atgtcaaaaa aatcaacgac catgccccgc cggtcaagtt tccgggcccg cagaacaatc
     7921 cgaaccttcg cgctatgtcc gtggagatga gaacatttgt cgtgcccgcc aagaagccgg
     7981 tggccgtaat tgaaataaag tgttttcgct acctggtaca ttccatgagc ctttcgataa
     8041 cttcttcgat gacatggttg ggggttgaag cgcccgccgt aattccaact ttagcggtcc
     8101 ctccgggaag ccatccgtcg gtttccgtga tcatttgagt atcaatgttt cgatgactga
     8161 ttttttcttt cgacaggata ccatcggcgt cttcaatatg gaacgtcggc gcgtacctgg
     8221 aagcgatctc cgccaaatgg ccggtgttac tcgatttgta accaccaaca acgagaatta
     8281 aatccatatg cttttcgccc agggcccgaa tggcgtcttg gcggtcctgg gtggccgaac
     8341 aaatggtgtc aaaatgacgg aaccgcgcgc gggcggcttc attcccgtag cgggcctcca
     8401 gcccttcccg aagcatcccc gaaatcacaa tcgattcgct catcagcatc gtggtttgat
     8461 tcgcaaaacc aatcttttca aggtccttcc ccgggtcaaa gccttccgaa catacccttt
     8521 taaaatctgc cagaaacagg tcgttttccc gaggggtatt tgacgccttg tcgcctcctc
     8581 tcaggatcaa atcgcaaact tgcctggcct gcgcctgatc tctcactacc agatatttcc
     8641 ccgcccgcga gcaggtcgcc ctcgtttctt catggtcgta ctttccgtgg atcacggaag
     8701 taaaaccgtc ccgggcgtaa gattccacgc gtcgccagac gctcatcacc gaaccgcagg
     8761 tcgtgtccac gatcacgcac ccgcgctcct gatactcttt caacgcgttg acaggcacgc
     8821 cgaatgccgg catgatcacg acatcttttt catcaagatc ggtcacggcc agtccgcagg
     8881 ccgaagggcc ggataaaaac cggatcccga gttcgcggag ctcacggttc actctcgggt
     8941 tatgaataat ttcatccgtt aaaaaaatcc tgcgatccgg gaatttcagg cgcgtctcat
     9001 acgcgtattc caccgcccgt tccaccccgt aacaaaatcc aaattccctg gcaagataaa
     9061 tctccagacg tccacttcgc aggcaataat cattggcgcg gattttttca atgagggggc
     9121 tcgaatcctg cttttcaagc aagggacgaa cgttgttttt cccccgggga acatcaccgt
     9181 ggggtttttc gcgagaatcc atggcgcgct agaccgaaag ctgtcggatc gcgaccccgg
     9241 cctgctgaag aagtttgaag gacgtgtcag aaagcggata agtcgcctga taaacaactt
     9301 cttgaatccc ggaattgata atcattttag tgcacatcag acacggagaa aaagtcgtat
     9361 agatcgttgc ccctttcacc gaaaccccgt gataggccgc ctgcacgatc gcgttttctt
     9421 ccccatgcga acaaatacac tccccgagat ccttcccgga agtttcaacc gacgcgcaac
     9481 ggggacagcc gccctcgttg caattcgtaa tcccccgggg agttccgtta taaccggtcg
     9541 agataatcct tttttctttc acgatcaccg ccgcgacctt tcgcttcatg cagttggacc
     9601 gcaatgccac cactttcgcg atattcatga aatattcatc ccatcccggc cgggtcgaac
     9661 tttgggcgat tttaacgaga agttgcctga gcttgtcgtg aagttctttc agcgggccgt
     9721 tattgtccac ctggagatcg gcgagcgcga gagtctgatc aagctgttgg tcggtcttgg
     9781 tttcactttt tgattctttg gcctcaagcg cctgaaaatc ttcccatgtt tcggggtcat
     9841 tctcccggcc gcgttgtttc atgcgctcga aacgaagcct ctcgggagca ttcaccctca
     9901 aaaatttaaa atctgccctg cggcggaaaa cctggacttc ggcggggtga cggatggaat
     9961 cgataatata atgtttttca ggatcaagct tcgcgtagat cctctccgcg agacaacccg
    10021 gcccttcttt ttcccgcaac tcattgccta acgccacaag cgtctcgcga gcgagaggtt
    10081 gaccgcgttt cccagcctct tcacgaagag cgtccgagag agaataataa tgaaaacccc
    10141 gttccttgag gaagttggcc gcctcccctt ttcccgatcc atttttcccc gttaatccga
    10201 taatcattga agatccttta tctgttgggc cccgttagaa aatttccgct ggtactcata
    10261 ccataagcca ttttttgtac ccaccacaaa aaatgatgct accctgtgcc ctctgggcta
    10321 aacaagcata agccattttt tgtacccacc acaaaaaatg atgctaccct gtttgcccct
    10381 tggactaaac aagcataagc cattttttgt acccaccaca aaaaatgatg ctaccctgtt
    10441 taggctttat tccagaaacc cggcgcgaaa taattcacgc ccgtcacgtg tcgttcgatc
    10501 agcaacaaaa cacttttttc ttttccgtcc tgaaaagccg gaatggcggc gatggcccga
    10561 atgtcttctc cgggtttccc ggcgcccggt ctcaaaaaac gaacggcctg tttttcaaga
    10621 tggaaagtcg cctcatcaat atccgcgacc ctgatcccca tcacataggg cgcgttttcg
    10681 ccaaaaacct tcccccactc ttttgactcc ggggccctgg tttcctcaat caagatggcg
    10741 ggaagacacc ggcaacggta aacatggaca ggatcgcgag gacggccgag aattctcgat
    10801 gaaatatccc gggtgtagcc cagctccgta aattcccggg cgcgttcttc cggattagcc
    10861 gagcgaaaca ggaaatggtc aagcacgggc caaagcccaa ctcccatcac ctccagcccc
    10921 cggaacacca ctcgcgccgc cgtattctcc gaagcaaaat ccccgatatg ttttttgagg
    10981 attttttcga gagccataat attcaactgc gcgaccgtgt gcggcatgac ggagatccgt
    11041 gttgcctcca gcgacgggaa agaccccgcc tcgccatttc gcgttctcgg caaaatgaac
    11101 cggcagggtg tttccggtta aaactttctt ttacttcagc tgattggtag gctttttggg
    11161 cgattcgttc ctggcccgag gagccacctt gggattggat ttaaattcaa ccgcgagatc
    11221 cgcgttaatt ttggcccagg tcggatccat atcttcggat tcgacgattg aacccacggg
    11281 acattctgcc gcgcaagcgc cgcaggaaat acaaaccgtc gggtcaatga ccataaaatc
    11341 tttcccctga taggtgccgg gatagatcgc ttcagtcggg cagaccgtcg cgcaaagacc
    11401 gtaacgttcg ccgtgacatt tctccgtaat aacgtaagcc attctcgatt tctccttttg
    11461 ggttgtgttg caaatgaaag ggtattgtag ccgcgccctc tggcatcgtc aatgttttag
    11521 gaaaatttca gagcaatttt tggaggtgtt tttgaatctt tctcacaggg aacacccacg
    11581 cctgccctta aggacaatcg gtagccccct cgggccacaa gtggggcggt tggctggaac
    11641 aaagacgctc atcactccgt ctggaatcac gatattgcgc ctcaagcctc gcgattctcg
    11701 cctcgacggg cgggtgggtc ctgaaaaagg aggtccacct ttgcccggaa agtggataaa
    11761 caataaaaag atgcgcggta gcgggttccg cgcttcttaa tggaaatttt tccgattcag
    11821 gtcccagttt ccgaagcgcg cttgccaggt agagtggatt tccacaccat gtcccgcctt
    11881 gacgatcagc tttatactca cggcgtcgtg ggataaatcc gcgcaccaaa accaccgcga
    11941 ccggaatcca aagtagcgtc agaaaaaaaa caaccggatg ggtctctccc cgttcataac
    12001 gttccccgct accccagtcc gcggatcgag gcaacatcgc cgcaatcatc ccgggcagtc
    12061 ccgtaatggc cgccacaatc gtagtgatca aaatatcatg actcatgata tgggaaagct
    12121 c
//
LOCUS       DAOSIO010000028        11721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_5472_length_11721_cov_4.897651, whole genome shotgun sequence.
ACCESSION   DAOSIO010000028 DAOSIO010000000
VERSION     DAOSIO010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 11721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11721
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5472_length_11721_cov_4.897651"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="PK590_01985"
     CDS             <1..755
                     /locus_tag="PK590_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOG23400.1"
                     /translation="LSWNVNGIRAVQKKGFLEWLAREKPDILGVQETKAKPEQLDIFL
                     MNPEGYHTYWNSAVRPGYSGVALFTREKPLKVTNGFGVKKFDEEGRVITAEYPDFAFL
                     TIYFPNGKASEERLQYKMDFYDETRRWVRRLRKKQPNIIVCGDYNTAHKEIDLARPKE
                     NEGVSGFLPIERAWMDAWVADGMVDIFREFHGGPWNYSWWDMKSGARERNVGWRIDYH
                     FVTESLVPKVRDASILKDVTGSDHAPVEIQLK"
     gene            837..1277
                     /locus_tag="PK590_01990"
     CDS             837..1277
                     /locus_tag="PK590_01990"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOG23401.1"
                     /translation="MKSLTKSILINAPLEKIWGLMADTSRTPEWVDGVRESERTGERR
                     EGKGFRWRESSILKNQNIDMEHEFTEWDPMTSARIKTSLPMNGWMDRVLKFTPGSDGV
                     QVKIDAQWDLGIAGMLLGEQKVFEVLQTSFEQTLLNWKEMVEKP"
     gene            complement(1323..3563)
                     /locus_tag="PK590_01995"
     CDS             complement(1323..3563)
                     /locus_tag="PK590_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23402.1"
                     /translation="MRRHPKIILASAVITGAFLFSSTLTATEPLSIDCLSVSAETGKV
                     IERYKGAGERTLIQIQDVHAHFTAQENIAAILEHLRTGFQVDKVALEGAWTSTELPQS
                     RAIPTSREKQLLARTLLENNLITGPIYAALLSPLPIQLTGIEDAELYEQNRTLYLAHL
                     EALPEINKKLAAYHERMIRSQKMLWNRDLLSFAGQVSKFRGSGDLGSYLPETLSAAAQ
                     HDISTADFDQILLIEEIMAKEKQLRGDRLQDEIRSLIRDFKNTPYSMEELLRGNKIPE
                     ERLGFYPEIKTLRELTVLKDRISLAALREQMDTLAETIVRALAKTEDELALWDKNKRF
                     FVARDILLLQATPDTLRLAEKELSLIREEFNSARLSRELELCLKFYAIVKQRDDIFMK
                     KILESPHLQGTLAVVTGGFHTDGLSEKLRKAGISYITISPELSNEKWSETLYEQRMTE
                     DLAAGLTTSALKEKDIASRENEEGKIVAREPATSVPEPSSSSPAPRTVHDQTLSDLRN
                     RLEVVDERFPPAFATLTATRNIQTALEVYQGRKTPAVSDASSPAHGQASGLGALLNHD
                     GAPLDFETFRRLPKEVQRSILANALEESSAISRPKIVVVARASVLKNLIDEKNAGPYV
                     RELVARDFLATAMDIPPATLPEELLGGPGIRRYEDSPGIAEVLSSQDLRRRTGKIPPV
                     VIDSEYESHRLLVLQASVPSLLLYRLLEPGSWLYEAAVKNPGFLSLLENLVAEILSEG
                     LVQRAA"
     gene            3890..>11721
                     /locus_tag="PK590_02000"
     CDS             3890..>11721
                     /locus_tag="PK590_02000"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOG23403.1"
                     /translation="MNSLKKLNKFLAGLVAVTFFATNTLTPSPLAFASVEPANPSRFA
                     DFKIPSEYGKVVDFAPALISGNLLRDSSFHGHQKDDSRILIHIQEAHANYDAQNNIRN
                     LLNYLAQNYGAKLIFLEGAGGKLQPERLNFFPDDPELQKAVTERLMKAGELTGAEAFL
                     IEKSGDANVQSLASNVSRSEGWGVENVDAYRSNWELYRSVYQKRETADRFLEDFYLQW
                     RKRASREFDKNLRDFILRYAEYEETKISLEAWIETLKSAAMAHLFLDVPAAEQRTPGK
                     RGTSDGSVPSAGMKMPLDLTDVHAQVNWPALVRYFRLKEAGKRIDETKVEIEKAGFLN
                     DISAGNGPFGASRSPALKVTDEVEEIFRSAKTGNLPVHKTRFVFERLMDVLPENFSFD
                     AYPNLRLYIQQIILTSEIDSRMLQSEMKELAKRILESLLKTDREKELVTELREYQQLK
                     KLFHLELSRDEYEREVRRNLTPEKLARLSGAARGDKRPAAGKTVMPAPNSALNVLFSE
                     AIRFYDGAIDREEKMMENAGKILGSRGDKAAVLITGGFHTDGFRKQITESGHSYIGIT
                     PNIGSVTREEEENYLRALLGTRGEQVTEKAIIQRSSSNVLPGNIVAPLAGNPDFMRQT
                     LGADYARNRFARIRNLVREVISALRPATSETLWARLDRTLRDLLSPAEMAGARSEARG
                     EDKKLKDQFEDQIQKHQKFIEQKINLFQRDPDVSKKYLRPNMETFQAGFFEIASFLRR
                     SLRVIRDIEPQVKRVYEMTMDWLRSMPDNFWSREAAPGVAEKTAKSVGILAAALRHLD
                     EYGSAITLLETFIRKTNIRNDVFLLSHLMLAHSSLATRYYAEKRTWDSRAEHLKSLEY
                     FALFLPLAEINDSLVRMLWTVKGTTLPVVFAIGRKDPARAQAVMEALERVNAAILEVL
                     ASSPRLDASIKSSLYEIQIRITEYLTSEQKKKETTVSVQPQEHKKTETVVDLPAQQTS
                     QPRIDKKIRWQQGEEERRKTGASRIQGGQRSFPGDEAAPKRRALEKVKEKLLGLLKAG
                     SEEDRELGKPGLQEEITSLNKPLGQEAVEVLSDFLWRVDEMTTEEQVYQVIDDALARS
                     ARSEIRDSVPEGNIGQKTEVSIIPPIAVRDIKTWMQAFELIRWIGNQPDITPNLSLIR
                     QLSLYLRERKKDLRLNTTRDYDQYPDDLAFGGRNAMLPLIRKAPTELSRSIKELALAL
                     PYAFSDTTLDSRSTLWGIAEDLDERTLRTKFARDLRGILHGPTFSYSVLPMVRRYINF
                     LETGDQGDGRFLTAEEIPVFLQDGDKAIQSHLSSLTLAERNRLMRDRVLLEQQEPGIR
                     EAVRVYGEIKELETALEILFETAAKPDTFKFWLNESFVKNEPPLKVALQTVVESFKEM
                     PFQRITKLINARAVVTETLDKTSDPGVRFSLYHLDRSIGQVMNLSLGTLLEAREGEPA
                     WEVEDALILIRNLLDELFDSGFITRYTVQWSDLLDDPDLSFSQLRDVLGVIEKGTRTE
                     FAKWAKDYHRDGEKIYGSEYKRLDQAIALMRKEKFATTAAMALLKAVKDRINQELPAH
                     EAKVARSESVEKKQGWLFEKITHIDDFKDSKQQFWGLERKVFGGKGTYLILMGSLLGL
                     PVPSAFILPSSMGKSLEIFEEKKKSFEDDILRNLRILEENWSRKMGRPFRYGAIPSDP
                     LNPEFEPLFLSIRSGSVFIMPGISETAVAIGFSPEIYEYILKTRGGQAGHKAFISFVE
                     SIGVSLGIPREKFENVIQKHISDARNLGLSYVDERILAQIVADMKDILKDGGVFDQFN
                     ELYKNPERQLLSLIPIIFQSWNSPKAKRYRQEKGISDDWHTPVIVQAYVFGDRDEQSG
                     SGTALSHDRTGEEAFNGEWIPMVQGMVHMQGRDTALSLNELEKRDPASYQVLKKGTML
                     LARHMGVPQMVEFTIEGGKPWFLQTVYDQRVGNAESFPDLDYEVVRHSSRVGGGNVAY
                     ENSGGLSGVVVFNPSDILREDILKKAADYDAVILASAFPTSDDSPDVINANRQLKKLA
                     GKRLIMLTQKGGLTSHAAVVANYEKIPTVVDVQGLDLGLESAALGGQPFFEGDVVTVD
                     FGTGGIYRGRIPQRSESRLASGEGHMIWPFQSVMHIAWEMAPFVKKGGLADVIGELPL
                     AMKRKYPGIDINVIIPYHRDINLGGVVPVSAGTFQYESPEGIQSWEAFKIEHHGLTVF
                     LLQRLSTGHQKFAPLFQKGYQTDNFYEGANFSKAAVEFMAQNNIRPDIIHPHDQTAAH
                     VPVILRDTRDAFFMESAVFYTAHHFAGAYQGKEDLGRARSYLMGLGLSARLFSEGGPV
                     VWGGKFNALRGASLYPDMVSTVSGHFAWEAANSDLSEGLQHVLQPRYVDTKTFLGITN
                     AIDKVMWSSRKGAGIQFPFEASTMEIGKRANKEYLQVKYGLKQDANAPLLSFVSRYDP
                     WVKGFDIVPAVVGELLNNYEELQVVFAGAGDKGVENALADLKKIYPDRVYVSPGFVDA
                     DTVRFILAGSDFVGMPSRIEPAGLVQQQGLLLGAVPIVTRVGGLEETVHDPFFTHGIG
                     VQTGFKSNLTDHLAMTFSWACVRALEMFRANKNLLTRIRLDGLDSVSSWDDRVDEYFE
                     AYTIALRNRQQFIHP"
BASE COUNT         2911 a         2800 c         3227 g         2783 t
ORIGIN      
        1 tattaagctg gaacgttaat ggaatccggg cggtgcagaa gaaagggttt ctggagtggc
       61 tcgcgcggga gaaaccggat atcctgggcg tccaggagac aaaggcaaag cccgaacagc
      121 tcgacatctt cctgatgaac cccgaaggtt accacaccta ctggaattca gcggtccgcc
      181 ctggctacag tggcgtggcc ctttttaccc gggagaaacc gctcaaagtg acgaatggtt
      241 tcggcgtgaa aaagtttgac gaggaagggc gcgtgatcac cgcggaatac ccggacttcg
      301 cgtttctgac catttatttc ccgaacggga aggcgagtga ggagcggctt cagtacaaaa
      361 tggattttta cgatgagacg cggcgatggg tccggagatt gaggaaaaaa cagccgaaca
      421 tcattgtctg cggcgattac aacacggcgc acaaggaaat agacctcgcg cgcccgaagg
      481 agaacgaagg ggtgtcggga tttctgccga tcgaacgggc ctggatggac gcgtgggtcg
      541 cggacgggat ggtggatatc ttccgcgaat tccacggcgg gccgtggaat tactcctggt
      601 gggacatgaa aagtggcgcc cgcgagagga acgtcggctg gcggatcgac taccattttg
      661 tgaccgaatc gctcgtcccg aaggtccggg acgcgtcgat ccttaaagat gtcactggct
      721 ctgaccacgc gccggtggaa atacaattaa aataaaatac gaggccgcgc gccacgcgcc
      781 acacggaaag ttttccgtct tgtgacgtgt gccgtcgtgg ttccgtgacg acaattatga
      841 aatcacttac taaatcaatt ttgatcaatg ccccgctcga aaaaatctgg gggctcatgg
      901 ccgacacgag ccgcacgccc gagtgggtgg acggagttcg ggaaagcgag cggaccggcg
      961 agcggcgcga gggaaaaggg tttcgctggc gcgagagctc catcctgaaa aaccagaaca
     1021 tcgacatgga acacgaattt acggaatggg acccgatgac ttccgcccgc attaaaacgt
     1081 cgctccccat gaacggctgg atggaccgcg tgttaaaatt cactcctggt tctgacgggg
     1141 ttcaagtcaa gatcgacgcc cagtgggacc tcggtatcgc ggggatgctt cttggcgagc
     1201 aaaaagtttt tgaagtcctc cagacgagct ttgaacagac cctcctcaat tggaaagaaa
     1261 tggtcgaaaa accctaggcc gcgagcgggg aattgatcgc gcggcttgtg ccgagtcgcg
     1321 tgtcaggcgg cccgctgaac aaggccctcc gataaaattt ccgccacgag attttcaagc
     1381 aaagacaaaa atcccggatt tttgaccgcc gcttcataaa gccatgagcc gggttccagc
     1441 agacggtaaa gcaacaggga aggaaccgac gcctgaagga ccaaaagccg gtggctttcg
     1501 tattcggaat caatcacaac gggagggatc ttccccgtac ggcgccggag atcctgactg
     1561 gataaaacct ccgcgatgcc gggactgtcc tcgtaacgcc tgatgccggg gccgcccaga
     1621 agttcttcgg gaagggtcgc gggaggaata tccatggctg tcgccagaaa atcccgcgcg
     1681 acaagttccc ggacataagg ccctgcgttt ttttcatcaa tcagattttt aaggacactc
     1741 gcgcgggcga ccacgacgat tttagggcgg ctgatcgcgg atgattcctc aagagcgttc
     1801 gcgaggatcg accgttgaac ttcctttggc aagcgccgga aggtttcgaa atcaagcgga
     1861 gccccgtcgt ggttcaggag agccccgaga ccggacgcct gcccgtgagc gggcgatgaa
     1921 gcatcagaaa cggccggcgt cttgcggccc tgataaacct caagcgcggt ctggatgtta
     1981 cgggtcgcgg tcagcgtcgc gaaagcgggc gggaagcgtt catcgacaac ctctagccga
     2041 ttacgcagat ccgaaagcgt ctggtcgtga actgtccggg gggccgggga agaagacgaa
     2101 ggctccggga cggatgtcgc gggctcccgt gccactattt ttccctcttc gttttcccgg
     2161 gacgcaatgt ctttttcttt taacgcgctt gtcgttaatc cagcggccag atcttccgtc
     2221 attctctgtt cgtaaagcgt ttcgctccat ttttcgttgc tcagttcggg agagatcgtg
     2281 atgtaagaga tcccggcttt tctcaacttt tcggaaagtc cgtccgtgtg aaaccctccg
     2341 gttaccacgg cgagagtgcc ctggagatga gggctctcca ggattttctt cataaaaata
     2401 tcatcgcgct gtttgacgat ggcgtaaaac ttcaggcaaa gttccagctc ccgggataat
     2461 cgggcggaat taaattcttc acgaatgagg gaaagctctt tctcggcaag acgaagggta
     2521 tcgggggtgg cctgaaggag aagaatgtcc cgcgcgacaa aaaaacgttt atttttatcc
     2581 caaagcgcca gttcgtcttc ggtcttggcc agcgcgcgga cgattgtttc tgccagggtg
     2641 tccatttgct ctcttaacgc ggcaagtgaa atccggtcct tcagcacggt caattcccgg
     2701 agcgttttaa tctccggata aaacccgagt ctttcttccg gaattttgtt gcccctcaac
     2761 agctcttcca tcgagtaagg cgtgtttttg aaatcccgga tcaaggacct gatctcatcc
     2821 tgaagcctgt ctccacggag ctgtttttcc ttggccataa tttcttcgat aaggaggatc
     2881 tggtcgaaat cagccgtcga aatatcatgt tgagcggccg cgctcaaagt ctcgggcagg
     2941 taacttccga gatcccccga tccccgaaat tttgaaacct gtccggcgaa agaaagaagg
     3001 tcgcggttcc agagcatttt ttgcgaacgg atcattcgtt catgataagc cgcgagtttt
     3061 ttattgattt cggggagggc ctcgagatgg gcgaggtaga gagttctgtt ttgttcatag
     3121 agttccgcgt cctcgatgcc ggtgagttgg atcggtaggg gggaaagaag ggccgcgtaa
     3181 atggggcccg tgatgagatt gttctccagg agagttctcg ccagcaattg tttttcccgc
     3241 gacgtgggaa tcgcccggct ctgggggagt tcggtcgagg tccaggctcc ctccagcgcg
     3301 actttatcaa cctggaatcc ggttcgcaaa tgctcgagaa tcgccgcgat attttcttgc
     3361 gcggtaaagt gggcgtgaac atcctgaatt tgaatcagag tcctttcgcc cgcgccttta
     3421 tagcgctcaa tgacctttcc ggtttcagcg gagacggaaa ggcaatcaat agaaagcggt
     3481 tccgtggccg ttagagtgga tgagaataaa aacgctcccg taattaccgc ggaggcaagg
     3541 atgattttgg gatgtcgtct catacgattt ctcccctcaa gttttaataa aacagaagtg
     3601 aagagcgcgc atagcaagaa gaaaataaag cgctgacacg ggtataacct aaccgagaaa
     3661 ttccaatcta tcaagttcaa tgctacgtta cgtctgacct tcagaaaaat agcaatttat
     3721 agcactggag cactaaggca atctaaagat taaatacaaa tacaaacttt aataaagtct
     3781 tttttaatac cttgaatttt aaagcaagtg tgttattttt cttgggtaag tttgttctta
     3841 ccgaaggttc gattgttaat ccaatatatc caatatatag aagacgcgca tgaattctct
     3901 aaaaaagctc aataaatttc tggccggatt ggtggctgtt actttcttcg cgaccaacac
     3961 tttgactcct tcgccgttag cctttgcctc ggttgaaccg gcgaacccgt cccgattcgc
     4021 ggatttcaaa attccttcgg aatacggcaa ggtcgtcgat tttgcccccg ccctgatttc
     4081 aggaaatctt cttcgcgact cttcgtttca tggccatcag aaggatgatt cccggatttt
     4141 gatccacatc caggaagccc atgccaatta cgacgcgcag aacaacatcc gaaatcttct
     4201 gaattacctc gctcaaaatt acggggcaaa gctgattttt cttgaggggg ccggaggcaa
     4261 acttcaaccc gaacgcctca acttttttcc ggatgatccc gaattgcaaa aagcggtgac
     4321 cgaacgcctg atgaaagccg gtgaattgac gggcgcggaa gcctttctga tcgaaaaatc
     4381 gggggacgcg aacgttcagt ctttagcctc gaacgtttcc cgctccgaag ggtggggcgt
     4441 tgaaaacgtg gacgcgtaca gaagcaactg ggagttgtac agatctgttt accaaaagcg
     4501 tgaaaccgcg gaccggtttc tcgaagattt ttacctccag tggcggaaac gggcgagtcg
     4561 ggagtttgat aaaaacttgc gcgacttcat cctccgttac gcggagtacg aagaaacgaa
     4621 gatttcgctc gaggcatgga tcgagacact gaagagcgcg gcaatggctc acttattcct
     4681 cgatgtcccg gccgcggaac agcggacgcc cgggaagcgt gggaccagcg atggctcggt
     4741 cccgtcggcc ggcatgaaaa tgccacttga tttgacggac gttcacgccc aggttaactg
     4801 gcccgcgctg gtgcggtact ttcgcctcaa ggaggctggc aagcggatcg atgaaacgaa
     4861 ggtcgaaatc gagaaagccg gttttttaaa tgatatttcc gccggaaacg ggccgtttgg
     4921 cgcttcccgc tctccggccc tgaaggtgac agatgaggtt gaggaaatat ttcgctccgc
     4981 gaaaaccggc aatttgcccg tccacaaaac ccgttttgtt tttgagcgtc ttatggatgt
     5041 tcttccggag aatttttctt ttgacgccta ccccaatctc cgtctttaca ttcagcagat
     5101 catcctgacg tccgagattg attcccggat gcttcaaagc gaaatgaaag agcttgcgaa
     5161 gcggattctt gaatcgctcc tcaagacgga tcgggagaag gaactcgtca cggaacttcg
     5221 ggaatatcag cagttaaaaa aacttttcca tctggagttg agccgggatg aatacgagcg
     5281 ggaggtgcgg cgaaacctca ctccggaaaa gttggccagg ctgtcgggcg cggcgcgtgg
     5341 cgacaaacgt ccggcggctg gaaaaacggt gatgcccgct cccaactccg cgctgaacgt
     5401 cctgttttct gaagcgatcc ggttttatga cggggcgatt gaccgcgaag aaaaaatgat
     5461 ggaaaacgcc ggaaagattc tcgggtctcg cggggacaag gcggcggtgc ttattaccgg
     5521 cggttttcac acggatggtt tcaggaagca gatcacggaa tccggtcatt cttatatcgg
     5581 gattacgccg aacatcggga gtgttacccg ggaagaggaa gaaaattatc tccgcgcgct
     5641 actcgggacc cgcggagaac aagtcacgga aaaagcaatc attcagcgtt cctcgtcaaa
     5701 tgttcttccc ggtaatatcg tcgctcctct ggcgggaaat ccggatttta tgcgtcagac
     5761 actcggagcc gactatgcca gaaatcgctt tgcccgtatc cgtaacctgg ttcgagaagt
     5821 aatttccgcg ttacgtccgg ctacaagcga aaccctttgg gcccgccttg accgaacgct
     5881 ccgcgatctt ctttctccgg cggaaatggc cggagcccgt tccgaagcgc gaggcgagga
     5941 caaaaagctt aaggatcagt ttgaagatca gattcagaag caccagaagt tcatcgagca
     6001 aaaaataaac ctgtttcaaa gagatcctga cgtttcaaaa aaatatctcc gtcccaacat
     6061 ggaaactttt caggccggtt tttttgaaat agcgagtttt ctgcgaagat ccctgcgggt
     6121 cattcgcgat attgaacccc aagtgaaacg ggtttatgaa atgacgatgg actggctacg
     6181 gtcgatgccg gataatttct ggtcgcgcga agcggctccc ggtgtagcgg aaaaaaccgc
     6241 aaaatcggtt ggtattttgg cggcggcgtt gcggcacttg gacgaatacg ggtccgcgat
     6301 cactctttta gaaactttta tccggaaaac aaatatccgg aacgatgtgt tccttctgtc
     6361 tcatctcatg ctggcccatt ccagccttgc cacccgctat tacgctgaaa aaaggacgtg
     6421 ggatagccgg gcggagcacc tcaagtcttt agagtatttc gccctttttc tcccattggc
     6481 ggagattaac gattcgctgg tgagaatgtt atggacggtg aaaggcacga ccttgcccgt
     6541 ggtttttgcg atcgggcgta aagatcctgc cagggcccag gcagttatgg aagcgcttga
     6601 acgtgttaac gcggccatac tcgaagtgtt ggccagctcg cccagattgg acgcatcgat
     6661 taaaagttct ctctacgaga tacaaataag aataaccgag tatctgacct ctgaacaaaa
     6721 gaaaaaagaa accactgtat ctgtccagcc ccaggaacac aaaaaaacgg aaacagtagt
     6781 tgatctgccg gcacagcaaa cctctcaacc ccgcattgac aaaaagatcc gttggcaaca
     6841 aggggaagaa gagcgccgaa aaaccggggc atcccgaatt cagggagggc agagatcgtt
     6901 tccgggggat gaagcggccc caaaacgccg ggctttggag aaggtgaaag agaaactcct
     6961 cgggttactg aaagcgggtt cggaggagga tcgtgaactc gggaaacccg gcctccagga
     7021 agaaattaca tctttaaaca aacctctcgg acaggaagcc gttgaggttt tgtcagactt
     7081 tttgtggcgg gtggatgaga tgaccacgga agaacaggtt tatcaggtca ttgacgacgc
     7141 gttggcccgc tcagcccgtt ctgaaattcg agactctgtc cctgagggaa atatcgggca
     7201 aaaaacagag gtttcgatta tcccgcctat cgcggtccgt gacattaaaa cctggatgca
     7261 ggcctttgaa ctgatccggt ggattggaaa ccagccggat ataactccga atctcagtct
     7321 gattcgtcag ctcagcttat atctccgtga gaggaaaaaa gacttgagat taaatacaac
     7381 gcgtgattac gaccaatatc ccgatgacct tgcgtttggc ggccggaatg caatgttgcc
     7441 gcttatccgg aaagctccca cggaattaag tcgctcgatt aaggagcttg ctctggctct
     7501 tccgtacgcc ttttcggaca ccactctcga ttctcggagt acgctctggg ggattgcaga
     7561 agatctggat gagaggaccc tccgtacgaa atttgcaagg gaccttcggg ggatattaca
     7621 cggcccgact ttttcttaca gcgttcttcc gatggttcgg cgatatatca actttctcga
     7681 aacgggagat caaggtgacg gacgtttcct tacggcagaa gagatccccg tatttctaca
     7741 agatggggac aaagcaatac aaagtcattt gagcagtctg accttggctg aaagaaacag
     7801 gttgatgaga gaccgggtat tgctggaaca acaggagcct ggtattcggg aagcagtgag
     7861 agtctatggg gaaatcaaag aattggaaac agccctcgaa attctttttg aaaccgcggc
     7921 aaaacccgac acatttaaat tttggcttaa tgaaagtttt gtaaaaaatg aacctccgtt
     7981 aaaagttgcg cttcaaacag tcgttgaatc ttttaaagaa atgccttttc agcggattac
     8041 caaacttatt aatgcgcgag ctgttgttac cgagacgctt gacaaaacgt ctgacccggg
     8101 cgtgaggttt tccctctatc atctggatcg aagcatcggg caggtgatga acttaagtct
     8161 ggggacattg cttgaggcac gagagggaga gccggcatgg gaagtggagg atgcgctgat
     8221 ccttatcagg aatctgttgg atgaactttt tgatagcggt tttattactc gatatacagt
     8281 tcagtggtct gaccttttag atgatccgga cctgagtttc agtcagcttc gagatgttct
     8341 gggcgtcatc gagaagggaa cccggaccga atttgccaaa tgggcaaagg attatcatcg
     8401 tgatggtgag aaaatatacg gttcggagta taaaagactg gatcaggcca ttgcgttaat
     8461 gagaaaagaa aaattcgcga ctacggcggc gatggcgctc ctgaaagccg tcaaggatcg
     8521 tatcaatcag gaactacccg ctcatgaggc gaaggtcgcc cgctcggaaa gcgttgaaaa
     8581 aaaacaaggg tggttgtttg aaaagataac gcacattgac gattttaagg attccaaaca
     8641 acaattttgg ggccttgaac gaaaagtttt tggcggtaag ggaacttatc tgatcctcat
     8701 gggttccctc ctaggccttc cggtccccag cgcgtttatc cttccttcct ccatgggaaa
     8761 atcgcttgag atttttgagg agaaaaagaa gtcctttgaa gatgacatct tgcggaatct
     8821 gagaattctg gaagaaaatt ggtcccggaa aatggggcgt ccctttcgtt atggcgctat
     8881 tccgtccgat cctttaaatc cggaatttga gcccctcttt ctttcgattc gctccggttc
     8941 cgtatttatt atgccgggga tctctgagac tgccgtggcg atcggattct ctccggaaat
     9001 ctatgaatat atcctcaaaa caaggggggg acaggcaggc cataaggctt ttatttcctt
     9061 tgtcgagtcg atcggagtga gccttggaat tcctcgtgag aaattcgaaa atgtgatcca
     9121 aaaacacatt tcagatgccc gaaatctcgg gctaagttac gttgatgaaa gaattttggc
     9181 ccaaatcgtg gctgatatga aagacatttt aaaagacggt ggagttttcg atcagttcaa
     9241 cgaattatat aaaaaccccg agcggcagct tctttctctc attcccatca tatttcaatc
     9301 ctggaactcg cctaaagcaa aacgttaccg tcaggaaaaa gggatttctg atgattggca
     9361 tacgcctgtc attgtccagg cctacgtttt tggagacagg gacgagcaat cgggctcagg
     9421 aacggctctt tcgcacgatc gaacaggtga ggaggctttt aacggagagt ggattcccat
     9481 ggttcaaggg atggttcaca tgcaggggcg ggatacagct ctttccttga atgagttaga
     9541 aaaaagagat cctgcaagtt atcaagtttt gaagaagggt accatgttat tagccaggca
     9601 catgggagtt ccgcagatgg tggagtttac gatcgagggc gggaaaccct ggtttcttca
     9661 aaccgtttat gaccagcgcg ttggaaacgc cgaatccttc cctgacttgg attatgaagt
     9721 tgtgagacat tcatctcgtg tcgggggcgg aaatgtggcc tatgaaaatt ccggcgggct
     9781 tagcggggtc gttgtcttca acccttctga tattttgcgc gaagacattt taaaaaaagc
     9841 agcagactac gatgccgtta ttctggccag cgctttcccc acatcggatg attcaccgga
     9901 tgtcattaat gccaaccggc aattgaagaa gcttgccgga aaacgcctca tcatgcttac
     9961 tcaaaaaggc ggtttgactt ctcatgcggc ggtagtggcg aactatgaaa agattccgac
    10021 ggtggtggat gttcaaggtt tggacctggg gcttgaatct gccgcgcttg ggggacagcc
    10081 gttttttgaa ggagatgtcg tgacagttga ttttgggacg gggggcattt atcgcggacg
    10141 tattccccaa cgctctgaat ctagacttgc ttccggagaa ggacacatga tatggccgtt
    10201 tcaaagcgtg atgcacatcg cctgggaaat ggcgcctttc gtgaaaaagg gcggtctggc
    10261 ggatgtgatc ggtgaattgc ctttggcgat gaaaagaaaa tatccgggga tcgatataaa
    10321 cgtgatcatc ccgtatcacc gtgacatcaa tctcgggggt gtcgtgcccg tttcggccgg
    10381 gacattccag tatgaatcgc ccgaaggtat tcagtcctgg gaagctttca aaattgaaca
    10441 ccacgggctt actgtctttt tgcttcagcg gctttcgaca gggcatcaaa aattcgcccc
    10501 gcttttccag aaaggttatc agacggacaa tttttatgag ggcgccaatt tttccaaagc
    10561 cgccgttgaa tttatggcgc aaaacaatat ccggccggac atcattcacc cgcatgacca
    10621 gacggcggcg cacgtgccgg tgatcctccg ggatacgagg gatgcctttt ttatggaatc
    10681 cgccgtgttt tataccgcgc accatttcgc gggcgcttat cagggaaaag aagatttggg
    10741 cagggcacga tcgtatttga tgggcctcgg cctcagcgcc aggctattta gcgagggagg
    10801 gccggttgtt tggggaggaa agtttaatgc gttgagaggc gcctcgcttt atccggatat
    10861 ggtttcgaca gtgagcggcc actttgcctg ggaagcggcc aactctgatc tcagtgaagg
    10921 gcttcagcac gttctccagc cgcgttacgt tgacacaaaa acatttctcg ggatcacgaa
    10981 cgcgatcgat aaagtcatgt ggtcttcccg caagggggcc gggattcagt ttccgtttga
    11041 agcttcgacg atggagatcg gcaagcgggc caacaaggaa tacctccagg taaaatacgg
    11101 attaaagcag gacgcgaacg cgcctctgct ttcgttcgtg agccggtatg acccgtgggt
    11161 gaaagggttt gacattgtac cggctgtggt tggggagctt ctcaataatt atgaagagtt
    11221 gcaggttgtt ttcgcgggag cgggggataa gggtgtcgag aatgccttgg ccgacttaaa
    11281 aaaaatctat cccgatcgag tctatgtctc cccggggttt gtggacgcgg acacagtccg
    11341 gtttattttg gccggttccg attttgtcgg aatgccttca agaatcgaac ccgcgggact
    11401 tgtccagcaa cagggccttc tgctcggcgc cgttccgatt gtgacgcgcg tcggcgggct
    11461 tgaggaaacg gttcatgatc ccttctttac ccacgggatc ggggtgcaga cgggtttcaa
    11521 gtctaattta acggatcatc tggccatgac tttttcctgg gcgtgtgtgc gggcgcttga
    11581 aatgtttaga gcgaacaaga atctgttaac acggatccgt ctggacggtc tggactccgt
    11641 gagttcctgg gacgaccggg tcgatgaata ctttgaagcc tatacaatcg ctctgcgaaa
    11701 tcgccagcaa tttatacacc c
//
LOCUS       DAOSIO010000029        11380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_5650_length_11380_cov_4.940132, whole genome shotgun sequence.
ACCESSION   DAOSIO010000029 DAOSIO010000000
VERSION     DAOSIO010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 11380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11380
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5650_length_11380_cov_4.940132"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PK590_02005"
     CDS             complement(<1..486)
                     /locus_tag="PK590_02005"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008285858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOG23404.1"
                     /translation="MTSRKISRTLGVNIDHVATLRQARRGFEPSVLEAAREARRGGAD
                     GITVHLREDRRHIQDKDVLELKRQIRLPLNLEMSIAPEILRIALKVRPEKACLVPEKR
                     QEITTEGGLDLFRKKRQLRRTIRALLHEEIEVSLFIDPDERQVREAAHLGASAIEIHT
                     GA"
     gene            complement(493..1434)
                     /locus_tag="PK590_02010"
     CDS             complement(493..1434)
                     /locus_tag="PK590_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23405.1"
                     /translation="MIPEWLRLNWGLKIISLILAVGLWYYALGEENIELTRVIPLEVT
                     IENPQLTIAKLSTDVIQVTFSAPRSLMVDLAGQEIRALHKIGPEIKAAGEYSFRVEHS
                     EILLPSFQARVLTIEPEVVTITLDEVIMQKLEIQPDFSGDPAIGYKLLKDEVRMDPNA
                     ILAQGPKSVLEAMKSVKTEPIDLVGRIRSFHRTMEVQLPEGVKPMSESLVDVYIPIRE
                     EFDEKEFRDVPVRVLRVPESNGVITIEPERVNFFLKGSKRYLEGLNAEDILAFVDLGT
                     MKKGDYDLPLQIVLPENVSLKDKEPIVIKTRISSAKD"
     gene            complement(1431..2249)
                     /gene="cdaA"
                     /locus_tag="PK590_02015"
     CDS             complement(1431..2249)
                     /gene="cdaA"
                     /locus_tag="PK590_02015"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOG23406.1"
                     /translation="MFQLIFSFWRPIVEILVIWAMVYSIIRFFQGTRAMQVLLGLLIL
                     VILFLVAKFFELNSIIWVMSKLFAVGVVAFLIIFQPEMRRALARIGTTMSGAFLQKNG
                     LMEEILRAVDYMSRQRIGALIAIEREVGLKNYIESGMMIDAKVSAELLWSIFDPHTPT
                     HDGGVIVVGDRIASCGSLFPLTQTLELPSSMGTRHRAAIGLTEENDAVCIVVSEETGN
                     ISVAVSGNLTRALDAENLKRILEGLFYPAENKNFLKEFIEKNRKIFRFQRQEPL"
     gene            complement(2251..2466)
                     /locus_tag="PK590_02020"
     CDS             complement(2251..2466)
                     /locus_tag="PK590_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOG23407.1"
                     /translation="MNDFARTLIFFGLILIGMGILAAFFSKIPMLGKLPGDIFIRRGS
                     FTFYFPITTCVLLSLILTLVLTIFGKR"
     gene            complement(2601..3743)
                     /locus_tag="PK590_02025"
     CDS             complement(2601..3743)
                     /locus_tag="PK590_02025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOG23408.1"
                     /translation="MKIGVLTLPLNDNYGGLLQAFALRTVLRSMGHDSIVIDRRRKRL
                     SNIRKFASIAKSRMLGRSIYPKLLLSEYQSGVILKEIKSFQKRYISHFTESITTDGGM
                     SCLNKMGFDAFVVGSDQCWRPRYLPNVRNYFLDFAENQRNIRRIAYAASFGVSHWEIN
                     EPDTRACRKLARKFDGISVREDTGVALVKKYLGRDDAVHVVDPTMLISAEQYMEIFAN
                     EDAPRVSGDLKVYILDKNQEKDLMTQQIAARFGWNLFEMQPHKRLGSEKVTDDNAGDF
                     SYPNPVQWVRGFRDAKFVVTDSFHGVVFSILFNVPFIAIGNVNRGLARFESLLSKLGL
                     SNRFVTDLSPDKINDAISQEIHWHRVNAIVENERSKSMDFLKKHLQ"
     gene            complement(3892..4608)
                     /locus_tag="PK590_02030"
     CDS             complement(3892..4608)
                     /locus_tag="PK590_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23409.1"
                     /translation="MKKLFIIGLTALLIPGILAGCSKAEQAKPLPFVEDQAGAVLPAG
                     SEEAVDEVRMIAEEIQTQSEEALEALETPVEEAEAQLAGLTALTENLKSESQEALAVL
                     EEKMDQVPEMEEVMEEVGMDAAEEAVEDLEEVIEDVDMDVAEEAVAGLEEKVAQVSDA
                     GAEAEGVMEDIDMDAVEENLRKLLAAEEAEEMNMEETESLTEEESDDLAAGSEPMEVG
                     NGTEEVGEATVAENSGTPIS"
     gene            complement(4659..5678)
                     /gene="ruvB"
                     /locus_tag="PK590_02035"
     CDS             complement(4659..5678)
                     /gene="ruvB"
                     /locus_tag="PK590_02035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOG23410.1"
                     /translation="MADDPLTNEEFKPEELDLEVTLRPATLDDFVGQSQVKENLKVFI
                     DAAIGRRESLDHILLFGPPGLGKTTLANIIAKKMEATIHQVSGPVLERAGDLAGILTN
                     LEKGDVLFIDEIHRIPHVVEEYLYSAMEDFSIEIMIDKGPHARSVKMQIPHFTLIGAT
                     TRSGLLTAPLRSRFGIVERLNYYDDEALATIVKRSAKILEIEIDHAGAREIAGRARGT
                     PRIANRLLRRVRDYAQVKAGGMITPDVADQGLKMLGVDKEGLDPMDKRILEYILKDCR
                     GGPVGVNSLAVGVGEDSETIEEIYEPYLIQRGFLKRTALGRIATERAYQHFQMTPPAA
                     EQQLF"
     gene            complement(5746..6348)
                     /gene="ruvA"
                     /locus_tag="PK590_02040"
     CDS             complement(5746..6348)
                     /gene="ruvA"
                     /locus_tag="PK590_02040"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOG23411.1"
                     /translation="MYYFLNGKIVEKTPAVAILDVNGIGYEVRISVNTFSALPDLGQS
                     VKLLTHFVVREDAQLLYGFATEEERDLFRLLLSVSGIGPKTALTLLSGMTISELKTAI
                     LDGSIAVLSSITGIGKKTAERVIVELRDKLTRGAGKDSKDIVHDMNVSDSAVEDAIQA
                     LVALGYTKLKAKEVTQRVLKDGLGKKLSVEDIIRKALKHV"
     gene            complement(6695..7180)
                     /locus_tag="PK590_02045"
     CDS             complement(6695..7180)
                     /locus_tag="PK590_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23412.1"
                     /translation="MCLSSFMMLLAFFAVLVLLVHSYGGQIRTVHANLETVKSFLKVP
                     EVEYKFFGIHFDLRGRYRGRKIVYTYRMSERRATAYYNISVEPKILLPEQKGFILDYP
                     RPTQNTQLVRGKIRYARRSLFGEGFFDPKLCLFSPGKVSEILEELSEVARRVESGEWR
                     P"
     gene            complement(7167..7688)
                     /gene="ruvC"
                     /locus_tag="PK590_02050"
     CDS             complement(7167..7688)
                     /gene="ruvC"
                     /locus_tag="PK590_02050"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOG23413.1"
                     /translation="MRVIGVDPGTHRAGIGILEADGAKYKIVYSGIIRANSKDPIAKR
                     LLHVFESLQTVIQSYHPEVMALETLFFAKDIQAVERIGEARACAMLAASKQGIEVIEY
                     APTSVKRSVTGNGRATKEQVQFMVMRLLNLKTAPSLDASDALAIALCHLHHRLSHKRM
                     LYKNLLQSEHVSK"
     gene            complement(7792..8538)
                     /locus_tag="PK590_02055"
     CDS             complement(7792..8538)
                     /locus_tag="PK590_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOG23414.1"
                     /translation="MSGHNKWASIKHKKAAADSKRGAAFTKIIREITVAAKSGGGDPT
                     MNPRLRTAIQAGKDANMPADNIERAIKKGTGELEGVTYEEVTYEGFGPGGTAMLVACL
                     TDNKNRTASEIRAIFGKNGGNMGGAGTVAWMFEKKGLIVVSHTGTTEDQLMEVAINGG
                     AEDMAAVGETFQVTTAPSDLWVVREAIEKAGIKIESANLVMNPKNMTAVDAGVARTVV
                     KLLEALEDHDDVQNVYSNADIPDEVMKELA"
     gene            complement(8663..9502)
                     /gene="folP"
                     /locus_tag="PK590_02060"
     CDS             complement(8663..9502)
                     /gene="folP"
                     /locus_tag="PK590_02060"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOG23415.1"
                     /translation="MIWKARERIASFDRTLLSGIVNVTPDSFSDGGKYLDPAKAVQHA
                     LELLADGADMLDLGAESTRPGSKSTSAAEEIKRLLPVLRKIRKQTDGIISIDTTKAEV
                     AKRCLDEGADAINDTSNLRVSGKKMVAAVKSFKAGLILMHSRGTPETMREMTEYQDLS
                     SEVLKEIAGSVEMALKGGVERERIMIDPGLGFAKNTEQNIAILRDLKPFLMTGYPVMV
                     GPSRKSFIGAITGRDACDRDFGTAACVASCAMQGIHVLRIHHVRGMRDVIKVTEAIKT
                     NNQ"
     gene            complement(9499..11331)
                     /gene="ftsH"
                     /locus_tag="PK590_02065"
     CDS             complement(9499..11331)
                     /gene="ftsH"
                     /locus_tag="PK590_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOG23416.1"
                     /translation="MPLGLLILLQVFVFPAMEMKEMNYSEFYRKLVANPETGEILSCE
                     LVDNVIHGKLITGSFFTVHIPINDLEIIPLMRRNIVDFSVSPPQTFWKNLIYSVMPVL
                     LLIGFFWFFVYRGMQQGGNRVFSFGKSRAKLVGKEKSPITFKDVAGVDEAKEELQEII
                     EFLKDPQRFTRLGGKIPKGVLLMGPPGTGKTLLAKAVAGEAAVPFFSISGSDFVEMFV
                     GVGAARVRDLFEQAKRAAKTGGKGAIIFVDEIDAVGRQRFAGIGGGHDEREQTLNALL
                     VEMDGFDTHAGVILIASTNRPDVLDPALLRPGRFDRQVVIERPDIKGREEILRVHSRT
                     VKLAAEVDLAKLARQTPGFSGADLANLINEAALLSARRKKDAVAQPELEESIERVMVG
                     PERRSRVISKREKRIKAVHESGHAIVALFTPNLDPVHKISIIPRGYGIGGYTLQVPDE
                     DRNLITRKELLDRIMVLLGGRVAEEIYFGEISSGASDDLEKATHYAQAMVCELGMSEK
                     LGNLTFGKKDRQVFLGRDLMREKDYSEQTAVLIDNEVRSIVDGCYARARDLITEHQDK
                     LRTLADALLEKEVLESDEIRQLVGLPVQEPATNDQPKTDEGPAT"
BASE COUNT         2712 a         3278 c         2577 g         2813 t
ORIGIN      
        1 agcgcctgtg tggatttcaa tggctgaggc cccgaggtgc gcggcttccc ggacctgcct
       61 ttcatccgga tcgataaaaa gactgacctc gatttcctca tgaagaagcg ccctgatcgt
      121 ccggcgaagc tgtctctttt ttctgaaaag atcgagtccc ccttccgtcg taatttcctg
      181 acgtttttcg gggacaagac aagccttttc gggcctgact ttgagcgcga tccgtaatat
      241 ttccggggca atcgacatct cgagattaag cggcagacga atttgccgct tcagctctag
      301 gacatccttg tcttggatgt gccggcggtc ttcccgtaaa tggaccgtaa tgccgtccgc
      361 gccccctcgt cgcgcctccc tcgccgcttc aagcacggag ggctcaaaac cccgtcgcgc
      421 ctgacgaagc gtcgcgacat gatcaatatt gacacccagt gttctggaga tctttcggga
      481 ggtcatggga gatcagtctt tggcggaaga aatcctggtt ttgatcacga tcggctcttt
      541 gtctttgaga cttacgtttt ccggaagcac gatctggagc ggcaaatcat aatccccctt
      601 cttcatcgtt cccagatcca cgaaggcaag aatgtcttcg gcgttcagtc cctcaagata
      661 acgttttgat cccttcagaa aaaaattgac ccgctccggc tcgatcgtga tcacaccgtt
      721 cgattccggg acacgaagaa cccggacggg aacgtcccgg aattcttttt cgtcaaattc
      781 ttcacgaatg gggatataga catccacaag cgattcactc atgggcttga cgccctccgg
      841 aagctggacc tccatggtcc gatgaaaaga acggatcctt ccgaccagat cgatcggttc
      901 cgtcttcacg gatttcatcg cttcaagaac gctcttgggc ccctgcgcaa gaatggcatt
      961 gggatccatc cgcacctcgt ctttcaaaag cttgtatccg atcgcggggt caccggaaaa
     1021 atccggctga atttcaagtt tttgcataat cacttcatca agcgtgatcg tcacgacctc
     1081 aggctcaatc gtaaggacgc gcgcttggaa actgggcaga agaatttcgg aatgttcgac
     1141 ccgaaacgaa tactcgccgg ccgctttaat ctcgggaccg attttgtgaa gggcgcgaat
     1201 ttcctggccg gcaagatcga ccatcaggga tcgcggcgcc gaaaacgtca cctggatcac
     1261 gtcggtcgag agcttcgcga tcgtcaactg cggattttca atcgtgactt ccaggggaat
     1321 cacccgcgtc agctcgatat tttcctcgcc cagggcgtaa taccagagac cgaccgccag
     1381 aatcaacgag ataatcttca acccccaatt cagacgcaac cattccggga tcataggggc
     1441 tcctgacgct ggaagcggaa tattttcctg ttcttttcga taaactcctt gagaaaattc
     1501 ttgttctcgg caggataaaa aagtccctca aggatccgtt taaggttctc ggcatccagg
     1561 gccctggtca gattgccaga cacggcgacc gaaatgttac ccgtttcctc ggagacaacg
     1621 atacaaaccg cgtcgttttc ttccgtcagg ccgatcgccg cacggtgacg ggttcccatc
     1681 gatgacggta gctccagcgt ctgggtcaac ggaaaaagcg atccacagga agcgatccgg
     1741 tcgccaacga caatcacgcc tccgtcatgg gtcggggtat gcggatcaaa aatactccag
     1801 agaagctccg cgctaacctt ggcgtcgatc atcatcccgc tttcaatgta gtttttaagc
     1861 cccacttccc gctctatggc aatcagcgcc ccgatacgct ggcgggacat gtaatccacc
     1921 gctctcaaaa tctcttccat gagaccgttt ttttgaagaa aagcgcccga catggtcgtt
     1981 ccaattctgg cgagcgcgcg tctcatttcg ggttgaaaaa taatcagaaa ggccactacc
     2041 ccgaccgcga acagtttgga catgacccag ataatgctat taagctcgaa aaatttcgcg
     2101 acaagaaaaa ggatgaccag aatcaacaac ccaaggagca cctgcatcgc gcgcgttccc
     2161 tgaaaaaaac gaatgatgga gtacaccatc gcccagatca cgaggatttc aacaatcggc
     2221 cgccagaatg aaaaaataag ctgaaacatt ttatctcttt ccgaaaatag tcaggacaag
     2281 ggtcaggatg aggctgagca aaacgcaggt cgtgatcgga aaataaaaag tgaaacttcc
     2341 gcgacgaata aaaatatccc ccggaagctt tccgagcatc gggatcttcg aaaaaaaagc
     2401 cgccagaatc cccatgccaa tcaaaatcaa cccgaaaaaa ataagagttc ttgcgaaatc
     2461 attcatgatc gcccgcctca agttgcgcta atataacaca gcccctccag acgaggcaac
     2521 gaatactggc cgattcctca ttcccctcct actaacgttt cggaccctat cgcgaggcga
     2581 aatggctacg gaaaaacaat ttattgcaaa tgctttttca ggaaatccat tgatttgctt
     2641 ctttcgttct ccacgatagc attcaccctg tgccaatgga tttcctgact gatcgcgtcg
     2701 ttaattttgt ctggagacag atccgtgaca aatcggttac tcaatcccag tttactgagc
     2761 aaagattcaa acctcgccaa tccgcgattt acgttgccaa tcgctataaa tggcacatta
     2821 aaaagaatcg aaaaaacgac accatggaat gaatccgtaa cgacaaactt ggcgtcccgg
     2881 aaccctctca cccattggac agggttgggg tacgaaaaat cccccgcatt atcatccgtt
     2941 actttttctg agcctagtcg tttgtggggt tgcatctcaa aaagattcca gccgaatctt
     3001 gccgctatct gttgcgtcat caaatctttc tcttgatttt tatccaagat gtacactttc
     3061 aaatcacctg ataccctggg agcatcctcg ttcgcgaaaa tttccatgta ttgttctgcc
     3121 gaaatcaaca tcgtcggatc aaccacatga accgcgtcat cccgccccaa atatttctta
     3181 accaatgcga cgccggtgtc ttctcttacg ctgatcccat cgaattttcg cgctagcttt
     3241 ctgcacgccc tggtgtccgg ttcatttatt tcccagtgag acacaccaaa tgacgcggca
     3301 tacgcgatcc gtcgtatatt tctttgattt tccgcgaagt ctaaaaaata attccgaacg
     3361 tttggcaaat aacgaggccg ccaacactga tcactcccca ccacaaaggc atcaaaaccc
     3421 atcttgttga gacacgacat cccaccatcg gtcgtaattg attctgtgaa atgggaaata
     3481 tatctcttct gaaagctttt tatctctttc aaaataacgc cagattgata ttcacttaaa
     3541 agcaacttgg ggtaaatgct tcgtcctaac atcctgcttt tggctatgga ggcaaacttt
     3601 ctgatgttgg ataaacgctt tcggcgccgg tcaatgacta ttgaatcgtg gcccattgat
     3661 cgcaaaaccg tccgcaacgc aaaagcttgc aaaagtccac cataattgtc attcagaggc
     3721 aaggtaagta cgccaatttt cacatcggat ccagagtttt acgtggttga ccatccactc
     3781 aaaataaaaa cctgttagga aatgttgcca ggttcgatgt tatggcaact tcattaaacg
     3841 cttgaagcaa gatcgccgct ggaatggagg ttccagcggc gatctgtcct tttaggagat
     3901 tggagttcct gaattttctg ctactgttgc ttccccaact tcctctgtcc cattgcccac
     3961 ttccatcggc tcagaaccgg ccgcgaggtc gtccgattct tcctcggtga ggctctcggt
     4021 ttcttccatg ttcatctctt cggcttcctc ggcagcaaga agcttcctga ggttttcttc
     4081 gaccgcgtcc atgtcgatat cttccatcac tccctctgcc tcggcgccag cgtcagagac
     4141 ctgagcgact ttttcttcta gacccgccac agcctcttcc gcgacatcca tatcaacgtc
     4201 ttcaattact tcttccagat cctccacagc ttcttccgcg gcatccatac caacttcttc
     4261 catcacttcc tccatctcag gaacctggtc cattttttcc tctaaaaccg ccagggcttc
     4321 ctgcgattca gacttaagat tttccgtcaa ggcggtaagc cccgcaagtt gcgcctcggc
     4381 ctcctcaact ggcgtttcaa gtgcctcgag agcttcttct gattgggtct ggatctcttc
     4441 ggcaatcatc cggacctcat caacagcctc ttccgaaccc gccggtaata cggcaccagc
     4501 ctgatcttca acgaacggca atggcttggc ttgttcagct ttggaacatc cggccaaaat
     4561 cccggggatc agcaaagccg tcaaacctat gataaacagc tttttcattg acttcctcct
     4621 ccttggttgg ttgaactaaa cattgataaa aaaacacttt aaaaaagttg ttgctccgcg
     4681 gcaggcggtg tcatctgaaa atgctgatac gcgcgctccg tcgctattcg tcctaacgcc
     4741 gtccttttca aaaacccccg ctggatcagg taaggctcgt aaatctcctc gatcgtttca
     4801 gaatcctcgc ctactccaac cgcgagactg ttcaccccga ccggcccccc ccggcaatct
     4861 ttcaggatat attcgagaat tcgcttgtcc ataggatcaa gaccttcctt gtcaacccca
     4921 agcattttca aaccttgatc cgccacatca ggcgtgatca ttccccccgc cttgacctgg
     4981 gcataatccc ggacacgccg gagcaaccgg ttggcaattc ggggagttcc tcgcgcgcga
     5041 ccggcgatct cccgggctcc cgcgtggtca atttcaatct ccagaatttt ggcggaacgt
     5101 tttacgatcg tcgcgagcgc ctcatcatca tagtaattca gtcgttcaac aatcccgaat
     5161 cgcgaccgga gcggggccgt caaaagcccg ctacgcgtgg tggccccgat aagggtaaaa
     5221 tgcgggattt gcattttcac ggagcgcgcg tgcggcccct tgtcgatcat gatctcgatt
     5281 gaaaaatctt ccatggccga gtaaagatat tcctccacca cgtgcgggat ccggtgaatt
     5341 tcatcgataa aaagcacatc ccccttctca agattggtca aaatcccggc aaggtcaccg
     5401 gcgcgttcaa gaacggggcc tgaaacctgg tgaatggtcg cttccatttt tttcgcgata
     5461 atattagcca aagtggtctt cccgagaccg ggagggccaa agaggagaat gtggtcgagc
     5521 gattctctgc gcccgatcgc ggcatcgata aaaaccttga ggttttcctt gacctgactt
     5581 tgcccgacaa aatcatccag cgtcgccggg cgaagcgtca cttcaaggtc aagttcttca
     5641 ggtttaaatt cctcatttgt caatgggtcg tctgccataa aattccttac acaagaaaca
     5701 caaaaaacgg tccgttggat catccttcac gtatggtgcc gtattctaaa cgtgtttgag
     5761 cgctttgcgg attatatctt cgacgcttaa cttcttccca agcccgtctt tcaaaactct
     5821 ctgcgtgact tctttagcct tcaacttcgt gtagccaagc gccaccagcg cctggatggc
     5881 atcctcgacc gccgagtctg acacgttcat gtcgtggacg atgtcttttg aatcctttcc
     5941 ggccccgcga gtcagtttat ctctcaattc cacgataaca cgttccgccg tttttttccc
     6001 aataccggtg atcgaggaaa gcaccgcgat tgaaccgtcc agaattgccg tttttagttc
     6061 ggaaatcgtc atcccggaaa gaagcgtcaa agccgtcttg ggaccaatcc ccgaaacgga
     6121 aagcagaagc ctgaaaagat cccgctcctc ctcggtcgcg aaaccgtaca gaagctgcgc
     6181 atcctcgcgc accacaaaat gcgtcaaaag tttgacgctc tgaccaagat ccgggagcgc
     6241 cgaaaacgtg ttcacggaaa tcctgacctc gtaaccgatt ccgttcacat ccagaattgc
     6301 cacggcgggc gtcttttcaa caatttttcc atttagaaag taatacattt ttcactttga
     6361 taaattcaat cacggtttct ttcccggagg gctcgccgcc atgatcgtcc atgtcaacgc
     6421 cgccagcgcc aggctccatt tcggctcaag aggataccat ctttttgaag aaaacaaata
     6481 aaaagtaccg cctgattttt atttatcaac ttttcctttt cgcggagtgc gagttgattc
     6541 atacccaatc ttgctatgaa gcaatattgg gggagaaacg cctgttagcg catcacttat
     6601 accagacgaa cagccctttt tttactgaat tcatgaaaaa tgcgagctga ctataaagac
     6661 tgtgactacg gggtcaatga gtgttttggg ctcgttacgg cctccattct cctgactcaa
     6721 cccgtcgggc gacttcgctt aattcttcaa gaatctcgct tactttcccc ggagaaaaaa
     6781 gacaaagctt gggatcgaaa aacccttcgc cgaacaagga acgcctggcg taacggatct
     6841 tcccgcgaac cagctgggta ttctgcgtcg ggcgcggata atccaggatg aaccctttct
     6901 gctccggcaa caagattttt ggctcgacgg aaatattata atacgcggtg gctctacgct
     6961 cgctcatccg gtaggtataa acgatctttc ggccccggta gcggccccgc aagtcaaaat
     7021 gaattccgaa gaacttatac tcgacctcgg gcactttcag aaaactcttg accgtctcca
     7081 gattggcgtg aacggtccgg atctgcccgc cgtaggagtg aacgagcaga accagcaccg
     7141 caaaaaacgc caagagcatc ataaaactac ttagacacat gttcggattg taacagattt
     7201 ttataaagca tgcgtttatg agacagccgg tggtgtaaat ggcacaaggc aatcgcgagg
     7261 gcatcggagg cgtcaagcga aggcgccgtt ttgagattta aaagtctcat gaccatgaac
     7321 tgcacctgct ccttggtcgc acggccattc cctgtcacgg aacgcttgac cgacgtcggc
     7381 gcgtattcga tcacctcaat accttgtttg gaagccgcaa gcatggcgca agcgcgcgcc
     7441 tccccgattc gttcaacggc ctggatgtct ttggcaaaaa agagggtttc gagcgccatc
     7501 acctcaggat gataactctg gataacggtc tggagagatt caaaaacgtg gagcaatctt
     7561 ttggcgatag gatctttgga gttcgcgcga atgatcccgc tgtagacaat tttatacttc
     7621 gccccgtccg cttcgagaat tccgatgccg gccctgtggg tcccgggatc aacacctatc
     7681 acgcgcatga catcaatgcc ccgcgcgtaa aggccgtgac ccgttgtccg cggttcacag
     7741 aaaaggaact tttttcaatt ccgccaaccg cgatcaggga accgcgaatc gtcatgcgag
     7801 ctctttcatc acttcatccg gaatgtcagc gttcgagtag acgttctgga catcgtcgtg
     7861 gtcttcaagc gcttcaagca acttgaccac cgtgcgcgcg actcccgcgt caacggccgt
     7921 catattcttg ggattcatga ccagattggc gctttcaatc ttgatccccg ctttttcaat
     7981 cgcttcccgt accacccaga gatctgaagg agcggtggtc acctggaatg tttctccgac
     8041 agccgccata tcctccgcgc ccccattaat cgccacttcc atcagttgat cttccgtggt
     8101 tcccgtatgg ctcaccacga tcaggccctt cttctcaaac atccaggcga ctgtcccggc
     8161 tccccccatg tttccgccgt ttttcccaaa aatagcccga atctcggacg ccgtccggtt
     8221 cttgttatcc gtaagacacg cgaccagcat cgccgttcca cccggcccaa atccttcgta
     8281 agtgacttct tcataagtca caccttccag ctcgcccgta cctttcttaa tggcgcgctc
     8341 aatattgtcg gccggcatat tcgcgtcctt gcccgcctga atagcggtac ggagacgcgg
     8401 gttcatggtg ggatcgccac ctcccgattt cgccgcgacc gtgatttcgc ggataatctt
     8461 cgtaaaagcc gcgcctcgtt ttgagtccgc ggccgctttc ttgtgcttga tactcgccca
     8521 cttattatgt ccggacattg tcttcctcct ctagcggatt caacagcctc atctcccgtg
     8581 aaaaatttca acgacgctca aaccctcgtc gttcaaaagc gttacgctct tcacatctca
     8641 caattggcga tttgggattc ggttattggt tatttgtttt aatggcttcg gtcactttaa
     8701 taacgtcacg cattccccta acatgatgaa tgcggagcac atgaattcct tgcatcgcac
     8761 atgacgcgac acacgccgcc gtcccaaaat ctcggtcaca agcgtcgcgt cccgtaatgg
     8821 ctccgataaa ggacttgcgg gaaggcccca ccatgacggg atagccggtc ataaggaatg
     8881 gttttaagtc gcgaagaatc gcgatatttt gttccgtatt tttggcaaac ccaagtcccg
     8941 ggtcaatcat aattcgttcg cgctccacgc cgcctttgag cgccatttcc acgcttcccg
     9001 cgatctcttt caaaacttcc gaagacaaat cctggtattc cgtcatttcc cgcatcgtct
     9061 ccggcgtgcc ccggctgtgc atcaaaatca gccccgcctt aaaactttta acggcggcaa
     9121 ccattttttt tcccgaaacc cgcaggttac ttgtgtcgtt gatcgcgtcc gccccttcat
     9181 cgaggcatcg cttcgccacc tcagccttgg tggtatcaat cgagataatc ccgtcggtct
     9241 gtttccggat cttccggagc acgggtaaaa gcctcttgat ttcttcggcg gcgctggtac
     9301 ttttcgagcc tggtctcgtc gactccgccc ccagatcaag catgtcggcg ccatccgcga
     9361 gaagttcgag cgcgtgctgg accgctttcg ccggatcaag gtactttccg ccgtcggaaa
     9421 aagagtccgg agtgacgttg acaattcccg acaggagcgt acgatcaaaa ctggctattc
     9481 tttcgcgggc tttccagatc acgtagcagg gccttcgtcg gttttaggtt ggtcattcgt
     9541 ggccggttcc tggacaggaa gtcctaccaa ctgccgaatc tcgtccgatt caagaacttc
     9601 cttttcaaga agcgcgtcgg ccaaggtgcg gagcttgtcc tgatgctccg taatgagatc
     9661 gcgcgctctc gcgtaacacc cgtcaacgat gctccggact tcattatcaa tcagaaccgc
     9721 cgtttgttcc gaatagtctt tctcacgcat caggtcgcgc ccgagaaaaa cctgccggtc
     9781 ttttttacca aaggtcaggt tcccgagctt ttcactcatc cccaactcac acaccatcgc
     9841 ctgggcataa tgcgtggcct tttcaagatc atccgacgcg cctgagctga tttcaccaaa
     9901 ataaatctct tcagcgacac ggcctccgag gagcaccata atccggtcca ggagttcttt
     9961 cctggtaatg aggtttcggt cctcatccgg gacctgaagc gtatagccac caattccata
    10021 cccccgcgga ataatagata tcttgtgaac cgggtcgagg ttcggggtaa agagggcgac
    10081 gatcgcgtga cctgattcat gaacggcctt gatccgtttt tcacgtttcg aaattaccct
    10141 gcttctgcgc tcgggaccga ccatcacgcg ctcaatcgat tcttcaagct caggttgggc
    10201 cacggcgtct tttttccggc gcgcggaaag cagagccgcc tcgttaatca gattggcaag
    10261 atcagctccg gaaaaacccg gcgtctgccg tgccagcttc gcgagatcaa cctccgccgc
    10321 cagtttcact gtccgggaat ggacacgtaa aatttcctcg cggcccttga tgtcggggcg
    10381 ttcgataacc acctgccggt caaagcgccc cggacgaagc aatgccggat ccagaacatc
    10441 aggacgattg gtcgacgcga ttagaatgac gccagcatgc gtgtcaaatc cgtccatctc
    10501 gacaagaagc gcgtttaggg tttgctcgcg ttcatcgtgt cccccgccga tacccgcaaa
    10561 acgctggcgg cccaccgcgt cgatttcgtc cacaaaaata atggctccct tacctcccgt
    10621 tttcgcggct cgcttggctt gctcaaaaag atcccgtacg cgcgcggccc ccacgcccac
    10681 aaacatctca acaaaatctg atccgctgat cgagaaaaaa ggtaccgccg cttccccggc
    10741 taccgctttg gcaagaagag tttttccggt cccgggaggt cccatcaaaa ggacaccttt
    10801 cgggatcttt ccgccaagac gcgtgaagcg ttgcggatct ttcaaaaact cgataatttc
    10861 ctgtaattct tccttggctt cgtcgacccc cgcgacgtct ttgaatgtaa tcgggctttt
    10921 ttctttcccc acaagtttgg cgcgcgactt cccgaagctg aacacgcggt tcccgccctg
    10981 ttgcatgccc cgataaacaa aaaaccagaa aaatccgatc aaaagaagca ccggcatcac
    11041 cgaataaata agatttttcc aaaaagtttg cggcgggctg accgagaaat cgacaatgtt
    11101 cctccgcatc agcgggataa tttcaaggtc attgatcggg atatgcacgg taaaaaaaga
    11161 tcccgtgatg agtttcccgt gaatcacgtt gtcgaccagt tcgcaagata gaatttcccc
    11221 cgtttccgga ttcgccacca gcttccggta aaactcggag taattcattt ccttcatttc
    11281 catggcaggg aacacaaaaa cctgcaaaag aatcaggagt cccaaaggca caagaaaaag
    11341 ccagattgtc ctgtttttat cgggctctgt gtttcttgga
//
LOCUS       DAOSIO010000030        11185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_5764_length_11185_cov_4.349596, whole genome shotgun sequence.
ACCESSION   DAOSIO010000030 DAOSIO010000000
VERSION     DAOSIO010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 11185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11185
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5764_length_11185_cov_4.349596"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PK590_02070"
     CDS             complement(<1..201)
                     /locus_tag="PK590_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23417.1"
                     /translation="MDSIPCPLSVCWIKPVKKLFLIPEKAVLANMLPHGFHQVYKKMD
                     VMQSNQLRAQNLMAVHQMPEIGP"
     gene            274..585
                     /gene="yajC"
                     /locus_tag="PK590_02075"
     CDS             274..585
                     /gene="yajC"
                     /locus_tag="PK590_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOG23418.1"
                     /translation="MFESIAHAAGETQTPQQVNPLVQFFPLIAIFFVFYFFLIRPQQQ
                     QQKKLREMVAGLKKGDKVITTGGILGEVSSIQPDYVVLSIGDSGAKLEVLKSAIAGLR
                     Q"
     gene            1022..3196
                     /gene="secD"
                     /locus_tag="PK590_02080"
     CDS             1022..3196
                     /gene="secD"
                     /locus_tag="PK590_02080"
                     /inference="COORDINATES: protein motif:HMM:NF009583.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOG23419.1"
                     /translation="MDKYYKWKVLLIVAATVFCLWKAYPLQEKINLGLDLQGGIQLLL
                     QVDMDKVPEEARKDVADRVVEIVRNRIDSFGVKEPDISKQGSSQVVVKLPGMTDRDRA
                     KEIVGKTAFLEFRLVSDDMDLLKKASDGQVPEGYEYKKIKSEAGDDMLLVSKEALLTG
                     AHLMTASVGFDNYGGAMVQLSFDDEGAKIFDRATFQNIGKRLAIVLDGTVHSAPVIRD
                     RIPNGRAQITGNFKAAEASDLALVLRAGALPAPVSVVEERTIGPGLGKDSIEKSVKAA
                     IFGVILVFLFMPVYYLLPGVVAAIGLVFYVIFMVGSIAALGATLTLPGIAGFILSVGM
                     AVDANVLISERMREEVVAGKGSRAAISAGYHKAFSAILDSNVTTLITSILLFIFGTGP
                     VQGFAVTLSVGIIASMFSALLVTRVIFDYLSKNNPNLNLRMHQMFKKTNIQFLKGRIL
                     AYGFSIITIGIGIAVFMTRGTQNYGVEFTGGTLTQLGFEEAVDVGDLRDALEEEGLKG
                     LTIQSYGEAKDNQVIVKMSGHETSRVEKAVRSVIGEKGCEILRVDQIGPSVSKDLTRK
                     ALWAVLWSTVGILIYLAWRFEWKFALAAVVALFHDTIFTFGIYALSGREINLPTIAAV
                     LTIMGYSVNDTIVTFDRVRDNLKLMRKTPFAEIVTLSINQTLSRTFLTSFTTILVTLA
                     LFIFGGAGINDFAFAMLVGFGVGIYSTIFVATSLVVDWKAHK"
     gene            3279..4922
                     /gene="recJ"
                     /locus_tag="PK590_02085"
     CDS             3279..4922
                     /gene="recJ"
                     /locus_tag="PK590_02085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOG23420.1"
                     /translation="MTSRAYSPVFQKLLEKRGFTDPRAIESFLHPDLSRLHDPFLMKN
                     MREVKARLETAIRDKEKILIHGDYDADGITGVAVMVKMLEKLGGDFITFLPERERDGY
                     GVSEEAIRKAHQENVRLLVTIDCGITAREEIRLARSLGIEVIVIDHHRLPADDLPDTN
                     FILNPLQEDCPYPFKDLSAAGLVFKLAQSMIGESAAEWLDLVAISTISDIAPLRGENR
                     TLAKFGLQALGERKNVGIQCLAEIAKLKSVKIDTGHVGFVLGPRINAAGRMSSPDTAL
                     RLLLTPSKREAASLANILNEENKARQRQEREITREAIAKVERTVNFNKERVIVVDGRE
                     WHQGVIGIVASRLVEKYERPAIVISVAGGVGKGSGRSIKGFHLFNALRSCAGKLEEFG
                     GHEQAAGLSILEKNIDGFRKSINEYAFANLDPAIMVKAVPIDLEIGLKDLTSVFIREL
                     ELMEPFGAGNPRPVFLTRRLQVKSGPRKVYGETYEVVVSGEGVNYSARINEKIMYRLA
                     AGRPEMKYDLAYSVRTRAWDGIETLSLDAKEMKESPDRN"
     gene            complement(4919..5182)
                     /gene="rpmB"
                     /locus_tag="PK590_02090"
     CDS             complement(4919..5182)
                     /gene="rpmB"
                     /locus_tag="PK590_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOG23421.1"
                     /translation="MKICVICDKKPLVGNSIARRGLSKKTGGIGLKTTGITRRRFMPN
                     LQKVKIRFPNGTIRRVNVCASCLQSGKVTKAPVRKKTETQAAG"
     gene            5322..5642
                     /locus_tag="PK590_02095"
     CDS             5322..5642
                     /locus_tag="PK590_02095"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HOG23422.1"
                     /translation="MARAIKKTTKTTKTAKTVATSEKAIEFKFFAPQAKSVCVGGNFN
                     GWKAEKTPMKKDANGNWKTSVVLRAGRHEYRYLVDGNWQNAQEPVECIPNAFGSWNCV
                     IEVR"
     gene            5675..7144
                     /locus_tag="PK590_02100"
     CDS             5675..7144
                     /locus_tag="PK590_02100"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOG23423.1"
                     /translation="MKILFVTSEMSPFAKTGGLGETVSALASAVCGKGHEVSVFMPRY
                     RCIDIQKWGLEIEINYFEVIIGNTLEATRVFSKKLDDGVKVYFIEHPDYFQRDDLYGT
                     PVGDYQDNDRRFTYFQRATLKAIEYLKLRPEVIHCHDWQTGLIPVYLKAVETKYAPFR
                     KAKSILTVHDFGFQGNFPPDSFPSTGLGWEYYKSDLLEFYSKFSFLKGGLVFADEVVT
                     VSEHYSREVQTKEFGSGMEGVLAARKDTVTGILNGLDVDAWDPSKDRDIESRYSMTTL
                     SKRKANKTLIQKEHGFAVDHEVPLIGLVSYLSTQKGLDILIPAFETFADMNIQVVLLG
                     TGEELYHQVLRDLAKRNKAWLGVHILFDLKMARQFYAGCDMMFFPSHHEPSSLGHLIA
                     CRYGAIPVAHATGCLADAIQPFDLTTGTGNGFLFEKDTTESLVDAVKRAVSVFKKEKL
                     WKALVKNAMGMDFSWSATAEQYIQLYETVKRRTVKVAKV"
     gene            7251..8330
                     /locus_tag="PK590_02105"
     CDS             7251..8330
                     /locus_tag="PK590_02105"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOG23424.1"
                     /translation="MKTYKIAVLPGDGTGGEVVREGNKVLSAAAEKFGFKLDRQEYDL
                     GGERYKRTGEVLPDSVLEELRGFPAIYLGAVGHPDVKPGILEKGLLLKLRFELEQYIN
                     LRPIKLFPGVECPLKGKGPEEIDFVVVRENNEGLYVGEGEFINKGTPDEVAVQVSRNT
                     RKGVERCLRYAFEYTRKRNSRKKTLTLCGKTNVLTYAFDLWDRVFHEIGAKDYPDVKR
                     DYAHVDATCMWMVKNPEWFDVIVTDNMFGDIITDLGAMIQGGMGIAAGGNINPKGVSM
                     FEPIGGSAPKYTGKNVINPLAAICAGQMLLEHLGEEAAARAIEDSVKWVTAKKLKSLA
                     AGKMGYSTTEVGDLVVKGLDPQNRS"
     gene            8361..9362
                     /locus_tag="PK590_02110"
     CDS             8361..9362
                     /locus_tag="PK590_02110"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013321868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOG23425.1"
                     /translation="MRKLNVAILGVTGAVGQEILNILEERKFPFASLKMLASGRSAGK
                     DLQFMGQTYRVEEATPDSFKRIDLVLSSAGGSISRALAPHAVKAGAVVVDNTSAFRMD
                     PEVPLVVPEINPEDIAKHKGIIANPNCSTIIMLVPLFPIHKVAKIRRIIAATYQSASG
                     AGAKAMEELRTQAQDFLAGEKVVPQVLPHPIAFNVFSHNSKIGPDGCCEEETKMVNET
                     KKIFHDDSVQVMATCVRVPVFRAHSESLFIETEKPVTADEARELLAKAPGVKVVDDRE
                     KNYFPMPLETTGQDDIFVGRIRTDPTVRNGLAMFVSGDQIRKGAATNTVQIAEFLVK"
     gene            9426..10187
                     /gene="truA"
                     /locus_tag="PK590_02115"
     CDS             9426..10187
                     /gene="truA"
                     /locus_tag="PK590_02115"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOG23426.1"
                     /translation="MTRNIKLVLEYDGAPFFGFQKQPGKPTVQQALEHALSRFFNQPM
                     KISAASGRTDTGVHAENQVVNFRTASDRDPGLIRKGLNAHLPPHIVVKSVEEVSPDFH
                     SRYDAKSKIYEYWVWNAPFRSPLLNARVFHVPFHLNLPEIRKAARRLVGRHDFSSFTS
                     GSALSGGPGKKKGGRVRTVKKISIQKKGDLLILQVEADGFLYHMVRNIVGTLLEVGRG
                     KRRPEEMAKILRAKSRRFSGSTAPAKGLTLRQVIY"
     gene            10340..>11185
                     /locus_tag="PK590_02120"
     CDS             10340..>11185
                     /locus_tag="PK590_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23427.1"
                     /translation="MNDLTKKIKTGLKNNPESKKDELIRKAVAAITTAFFIFSGVPVP
                     TSFANSHQTVMNELNQPVSTVTLEQANAAAPATRGSIPSGAAIPADNPLSSPTPESEP
                     EDPEFRETIYILPSSQAERVADIDSLMELLASKGITRITRDQIEALKPAQTDRMHIWG
                     AAYHVSQNGDQIAIAFSAVKEYGDTVMIKGVVPSWHPYSRSYFSNILYSASPDSDPVV
                     IDRSACVAHMGFTADGLLAIEGETGGSSVYNSEGEVVAFANHVVELYPVPSGWTRAAS
                     NANYAF"
BASE COUNT         2711 a         2634 c         3153 g         2687 t
ORIGIN      
        1 agggcctatc tcaggcatct ggtgaacagc catgagattt tgggctcgca attgattact
       61 ttgcataacg tccatttttt tgtaaacttg gtgaaatcca tgcggaagca tattcgccaa
      121 aacagctttc tcaggtataa gaaacagttt tttaaccggt ttgatccaac acacagatag
      181 ggggcagggg atagagtcca aggaacagga aaagattttc cgtgtcccgg acgtcgcgtc
      241 ctctacgctc tagcggaacc ttaggagttt aaaatgtttg agtccatcgc gcacgcggca
      301 ggagaaacgc aaaccccaca gcaagtgaac ccgttagtcc agtttttccc tctcattgcc
      361 atttttttcg tgttttattt ttttctgatc cgtccccagc agcaacagca aaagaagctc
      421 agggagatgg tggcgggact caaaaaaggc gacaaggtta tcacgaccgg cggcattctg
      481 ggagaggtgt ccagtatcca gccggattac gtggtgttga gcatcgggga ctcgggcgcc
      541 aagcttgaag tgctcaagag tgcgatcgcg gggctcaggc aataaaaaag ttgtaagcgg
      601 taaggtacga tgggaaagaa agcggttcga atatttaaag acttggttgt ttggcaaaag
      661 gcgcgtgagt tgaccttggc tatttatcag atcaccaagg cattccctca gacgaaaaat
      721 tcgggatcat tagccagctg agacgcgcag tcagttcagt tgccaccaat gttgtcgaaa
      781 ggcatataaa gaaacagccg gaaggatttt tcgcgctttt tgaatttggc cgacgcgtcc
      841 ttggaggaaa caaaatatca cttgatgttg ggcaaagatt tgagatatct ggatggcagc
      901 cattttgatc aattaatttt aaagtgcgaa gaagttgggc gcattctttt tggttttcag
      961 aagagcttaa aagttcattc ttatcgctta acacttaacg cttaacgcta gaaaggaaaa
     1021 catggataaa tattacaagt ggaaagtgct tttgattgtg gcggccacgg tgttttgcct
     1081 ctggaaggcc tatcctctcc aggagaaaat taatctcggc cttgatctcc agggtggaat
     1141 acagcttctg ttacaggtgg acatggacaa agtccccgaa gaagcgagaa aggacgtggc
     1201 ggaccgcgtg gtggagatcg ttcgtaaccg gattgactcc tttggtgtca aagaacccga
     1261 tatttccaag cagggaagtt cgcaggtggt agtgaaactt cccggcatga ccgaccgcga
     1321 tcgcgccaag gaaatcgtcg gcaagacggc ttttcttgag tttcgtctcg tttcggacga
     1381 tatggatttg ctcaagaagg cttcggacgg acaagttccg gaaggatatg agtacaagaa
     1441 aatcaagtcg gaagcgggcg atgatatgtt gctggtttcc aaagaagccc ttttgacggg
     1501 ggctcatctg atgacggctt ccgtgggttt tgataattac ggcggcgcga tggttcagct
     1561 aagttttgac gatgaaggcg ccaagatttt tgacagggcg acgttccaga atatcggaaa
     1621 gcgtctcgcg atcgtgctgg acgggacggt tcattcggcc ccggtgattc gcgaccggat
     1681 tccgaacggg cgcgcccaga ttaccggaaa tttcaaggcc gctgaagcca gcgatctcgc
     1741 gctcgtgctt cgagcgggcg cccttccggc tccggtgtcc gtggttgaag agaggaccat
     1801 cggcccggga cttggcaagg attccatcga aaagagcgtg aaagcggcca ttttcggcgt
     1861 gatcctcgtt ttccttttta tgccggtcta ttatcttctg ccgggtgtgg tggccgccat
     1921 cggacttgtt ttttacgtca tttttatggt cggttccatc gcggcgctcg gggcgactct
     1981 gacccttccc ggtattgccg gtttcatttt gtcggtggga atggcggtag acgcgaacgt
     2041 gctgatttca gaaagaatgc gcgaggaagt tgtcgcgggg aaaggttccc gggcggccat
     2101 ttcagcgggc tatcacaagg ccttttcggc cattcttgac tccaacgtga cgacgctgat
     2161 tacctcgatc ctcctgttca tttttggtac gggccctgtc cagggatttg ccgtaactct
     2221 ctctgtcggt attatcgcga gcatgttttc ggcccttctc gtcacccggg tcatttttga
     2281 ttatctgagt aaaaacaatc cgaacctgaa tcttcgcatg catcagatgt tcaaaaagac
     2341 caacatccag tttttgaaag gacgcatcct ggcctacggt ttttccatca tcacgattgg
     2401 aatcggaatc gcggtgttta tgacgcgggg aacccaaaac tacggagttg aattcaccgg
     2461 cgggacccta acccagcttg gttttgaaga agcggtggat gtgggtgatt tgcgggacgc
     2521 tcttgaagaa gaggggttga aaggtttgac gatacaaagt tacggtgagg ccaaggacaa
     2581 tcaagtgatc gtaaaaatgt caggtcacga aacctccagg gtcgaaaagg cagtccggtc
     2641 cgtgatcgga gaaaagggct gtgaaatctt gcgagtcgac cagatcggcc cttccgtgag
     2701 caaggatttg acccgaaaag ccctttgggc cgttctttgg tccacggtcg ggattttgat
     2761 ctatctggcg tggaggtttg aatggaagtt cgcgctcgcg gccgtagtcg ctcttttcca
     2821 tgacacgatt tttacgtttg gaatctacgc gctttcagga cgcgagatca atcttccgac
     2881 gatcgcggcg gtgcttacca tcatgggata ttccgtcaat gacacgattg tgacttttga
     2941 ccgtgtccgc gacaacctga agctcatgcg aaagacgccg ttcgcggaaa tcgtgaccct
     3001 gagtatcaat caaacgctga gccggacgtt tctcacgagt ttcacgacga ttctcgtgac
     3061 cctcgcgctg ttcattttcg ggggagccgg aatcaatgat ttcgcgtttg ccatgctggt
     3121 tgggttcggc gtcggcatct actcgacgat ttttgtcgcg acgtcgcttg tggtggattg
     3181 gaaagcccat aagtaagcaa taagcggtaa gcgtaaagcg ataagttttt ttcttaacgc
     3241 ctgacgctta ccgctttacg cttttaaatt gttgaagcat gacctctcga gcttattctc
     3301 ccgtttttca aaaactcctc gaaaagcgcg gttttaccga tccgcgggcc attgaatctt
     3361 ttttacatcc cgacctctcg cgtctccacg acccttttct catgaaaaac atgcgggaag
     3421 tcaaggcgcg ccttgaaacg gcgatccgcg acaaagaaaa aatcctgatc cacggggact
     3481 atgatgccga cggcattacg ggcgtcgcgg tgatggtgaa gatgcttgaa aaacttggcg
     3541 gtgattttat cacgttcctt ccggagcgtg agcgtgacgg ctacggcgtc agtgaggaag
     3601 cgatccggaa ggcgcatcag gaaaatgtga gactgcttgt cacgattgac tgcgggatta
     3661 cggccaggga agagatccgg cttgcccgga gtctcggtat cgaggttatt gtgattgacc
     3721 atcaccgttt gccggcagat gaccttcccg acacaaattt cattttgaat cctttgcagg
     3781 aagattgtcc ttaccctttc aaggatctct ccgcggcggg gcttgttttc aaattggcgc
     3841 aaagcatgat cggagaatcg gccgcggaat ggctggatct cgtggcgata tcaacgatca
     3901 gcgatatcgc gccgcttcgg ggtgaaaatc gaacgctggc aaaattcggg cttcaggcgc
     3961 tcggcgaacg gaaaaacgtc gggattcagt gtcttgccga aatcgcgaaa ctcaaaagtg
     4021 tcaagattga tacgggccac gtcgggtttg tcctggggcc caggattaac gcggcggggc
     4081 ggatgagttc ccctgacacg gcgctccgtc ttttacttac cccgtcgaaa cgggaggccg
     4141 ccagtcttgc caatatcctg aatgaagaaa acaaagcgcg ccagcggcag gaaagagaga
     4201 ttacccggga agcgattgcg aaggtggaac ggacggtcaa ttttaacaaa gagcgggtca
     4261 ttgtcgtgga cgggcgggaa tggcatcagg gagtgatcgg gattgtggca tcgaggcttg
     4321 ttgaaaagta tgaacgtccc gcgattgtca tttcggtggc gggcggtgtc ggaaaggggt
     4381 ccggacggtc gattaagggg tttcatcttt ttaacgcgct acgatcttgc gcggggaagt
     4441 tggaagaatt tggcgggcac gaacaggcgg cggggctttc cattctggaa aaaaatattg
     4501 atgggttcag aaaatcgatc aatgaatatg cttttgccaa ccttgatccg gcgatcatgg
     4561 tcaaggcggt gccgatcgac cttgaaatcg gattgaagga cttgacctcc gtgtttatac
     4621 gggaactgga gctgatggaa ccgttcggag cggggaaccc gcgtcccgta tttcttacgc
     4681 gacgacttca ggtcaaatcg gggccgcgta aagtttacgg ggaaacttat gaagtggtgg
     4741 tttcgggaga aggggtgaat tattcggccc ggattaacga gaaaataatg taccgtctgg
     4801 cggccgggcg tccggaaatg aaatacgatc tggcgtattc ggtcagaacc agagcctggg
     4861 acggaataga gacactgagc ctcgatgcga aagaaatgaa ggaatcgcca gaccggaact
     4921 aaccggcggc ttgagtttcc gttttttttc ggaccggcgc tttggtgact ttgccggact
     4981 gaagacagct cgcgcagaca ttaacccgcc gtatcgtccc gttcgggaac cggatcttga
     5041 ccttctgaag gttcggcatg aaccgacggc gcgtaatgcc cgtggttttt aaaccgatcc
     5101 cccctgtctt tttcgaaaga ccccggcgag cgatagaatt tcccactaaa ggttttttgt
     5161 cgcagatcac gcagattttc atagaggcgg tattatagtg agcagttgct gcttggcaag
     5221 ctgttttttc tgatatatac tcctcgcact tccggtactt ctaatttttc agaacccggt
     5281 ttaaccaatc tccctacgta aaaaaaggaa ggagttctct catggcccga gcaatcaaaa
     5341 aaacgactaa gacgactaaa acggcaaaaa cagtagcgac ttcagagaaa gcgatcgaat
     5401 tcaagttttt tgccccccag gcgaagagtg tttgcgtggg tggtaatttc aacggctgga
     5461 aggcggaaaa aacgcccatg aaaaaggacg ctaacggtaa ctggaaaact tccgttgtcc
     5521 ttcgcgcggg acgtcacgaa taccgttacc tggtggacgg gaattggcaa aacgcccagg
     5581 aaccggtcga gtgtattccg aacgccttcg gtagctggaa ctgcgtgatc gaagttcgat
     5641 aagcgtcatc aagaccgccg actaaaccac tttcgtgaaa attctctttg ttacctccga
     5701 aatgtccccg tttgccaaga cgggggggtt gggtgagact gtctccgcgc tggcttccgc
     5761 tgtttgcggc aagggacacg aggtctctgt tttcatgccc cggtatcgtt gtatcgatat
     5821 ccagaagtgg gggcttgaga tcgagatcaa ctatttcgaa gtgattatcg ggaacaccct
     5881 cgaggccacc cgggtttttt ccaaaaaact cgatgatggt gtcaaagtct actttatcga
     5941 acatcccgac tactttcagc gcgacgatct ctacggaact ccggtcgggg attaccagga
     6001 caacgacagg cggttcacct attttcagcg ggcgacgctg aaggcgatcg agtacctcaa
     6061 attgcggccg gaggtgattc attgccacga ctggcagacg ggattgattc cggtgtatct
     6121 caaggcggtc gagacgaagt acgcgccctt tcgcaaggca aaaagcattt tgacggttca
     6181 cgatttcgga tttcagggga attttccgcc agactctttt ccttcgaccg gactgggttg
     6241 ggaatattac aaatcggatc tccttgagtt ttacagcaaa ttcagttttc tcaaaggcgg
     6301 tcttgttttt gccgatgaag tcgtgacggt gtcggaacat tacagccggg aagttcagac
     6361 gaaagaattt ggctccggta tggagggcgt tctcgcggcc aggaaggaca cggtcacggg
     6421 gattttgaac ggactggacg ttgacgcgtg ggatccgtca aaggatcggg acatcgaatc
     6481 ccgttacagt atgacgaccc tctccaaaag aaaggccaat aaaacgctga tccagaagga
     6541 gcacgggttc gcggttgatc acgaggttcc gctgatcggg ctagtgtcct acctgtcgac
     6601 ccaaaagggc cttgatattt tgatccccgc gtttgaaacg ttcgcggata tgaacataca
     6661 ggtcgtttta ctcgggacgg gcgaagagtt gtatcaccag gttcttcgtg atctcgcgaa
     6721 acgaaacaag gcctggctcg gcgttcatat tcttttcgac ctgaaaatgg cacggcaatt
     6781 ttacgccggt tgcgacatga tgttttttcc gtcccaccat gagccgagca gtttgggaca
     6841 tctgatcgcg tgtcgttacg gcgccatacc agtggcgcac gccaccggtt gtttggcgga
     6901 cgcgatccag ccttttgacc taacaaccgg cacgggaaac ggttttctct tcgagaagga
     6961 tacgaccgaa tcgctggtgg acgcggtgaa acgtgccgtg tcggttttta aaaaagagaa
     7021 gttgtggaaa gcccttgtca aaaatgcgat gggaatggat ttttcgtggt cggcgacggc
     7081 ggaacaatat attcagcttt acgagacggt gaaacgccgt accgtcaagg tagccaaggt
     7141 ctgatttctc aaagaacgtt ccttccttga aactggttca gctttgggcc gtcatggttg
     7201 agaaggcgat ttttacttaa aaagttcacc tacaatagga gatcataaaa atgaaaacat
     7261 acaagatagc cgtactcccc ggagacggaa cgggtggcga ggtggtgcgc gaaggcaata
     7321 aggtcttgtc ggccgccgcg gaaaagttcg ggtttaaact ggatcggcag gaatacgatc
     7381 ttggcgggga acgttacaaa cgaaccggtg aagtgcttcc cgacagcgtg cttgaggagc
     7441 ttcgcgggtt tccggcgatt tacctcggcg cggttggaca tccggacgtg aagcccggta
     7501 ttctcgagaa agggcttttg ctgaaacttc gtttcgagtt ggaacagtac attaatcttc
     7561 gtcccatcaa gcttttcccg ggagtggaat gtcccctaaa aggcaaaggt cccgaagaga
     7621 ttgatttcgt ggtggtgcgc gaaaataacg aggggcttta tgtcggagaa ggtgaattta
     7681 tcaataaagg aaccccggac gaagtcgcgg tccaggtgag ccgcaatacc cgaaagggcg
     7741 tcgagagatg tttgcggtac gcgtttgaat acacgcgcaa acgcaattcc aggaaaaaga
     7801 cgctcacgct ttgcggcaag actaacgttt tgacgtacgc gttcgatctt tgggaccggg
     7861 tttttcatga aattggagcc aaggattatc cggacgtgaa acgggattac gcgcacgtgg
     7921 acgccacgtg catgtggatg gtcaaaaatc cggaatggtt cgacgtgatc gtgaccgaca
     7981 atatgtttgg cgatattatc acggatcttg gcgcgatgat tcagggaggg atggggatcg
     8041 ccgcgggcgg caacatcaat cccaaagggg tctccatgtt cgaaccgatc ggcggatccg
     8101 cgcccaaata caccggcaag aatgttatca atccgctggc ggcgatttgc gcggggcaga
     8161 tgctactgga acatctaggc gaagaagcgg ccgccagagc gatcgaggat tccgtcaagt
     8221 gggttaccgc gaagaaactc aagagtctcg cggccgggaa aatgggttat tccacgaccg
     8281 aagtcggaga tttggtggtc aagggactgg atcctcaaaa tcggtcgtaa aatcctaatg
     8341 acaaaaagga gatttgaaac gtgagaaagc tgaatgttgc gatcctcggt gtgactggcg
     8401 cggtgggcca ggaaattttg aatattcttg aagaacggaa atttccgttc gcgtctctca
     8461 aaatgctggc ctcgggacgt tccgcgggca aagatcttca gtttatggga caaacttaca
     8521 gggtcgagga ggcgacgccg gattccttta aaaggattga cctcgtgctt tccagcgccg
     8581 gaggttcgat ttcccgggcc ctcgcgccgc acgccgtcaa ggccggagcg gtggtggtag
     8641 ataataccag cgcgtttcgc atggatcccg aggtccctct cgtggtgccg gagattaatc
     8701 ccgaagacat cgccaaacac aaggggatta tcgcgaatcc gaactgttct accattatca
     8761 tgctggtccc gctttttccg attcacaagg tcgcgaaaat tagacgtatc attgccgcga
     8821 cgtaccagtc cgcgagcggc gccggagcga aggccatgga agaactccgg acccaggcgc
     8881 aggatttcct tgccggggaa aaggtggttc cgcaggtgct tcctcatccg atcgcgttta
     8941 atgttttttc ccataactcg aaaatcggtc ccgatgggtg ctgtgaggaa gaaaccaaga
     9001 tggtgaacga gacgaaaaag atttttcatg atgattcggt ccaggtgatg gccacctgtg
     9061 tccgcgtgcc ggtgttccgg gcgcattccg aatctctctt tattgaaact gaaaaacccg
     9121 tgacggcgga tgaagcgcgg gaactgctcg cgaaagctcc cggggtcaag gttgtcgatg
     9181 accgggagaa aaattatttt ccgatgcccc ttgaaacaac ggggcaggac gacatttttg
     9241 tcggacggat ccgcacggac ccgacggttc ggaatggtct cgcgatgttt gtttcgggag
     9301 accagattcg caaaggcgcc gcgacgaata cggtgcagat cgcggagttc cttgtcaaat
     9361 aggccgcaag ccacaaggac gcgcaagcgc gtgtgagtct tgggcgtgtg gcggatgtct
     9421 cgtttatgac gcgtaacatc aaactggtac tcgaatatga cggcgctccg ttttttgggt
     9481 tccagaagca acccggaaag ccgacggtcc agcaagccct tgagcatgcc ctatccaggt
     9541 tttttaacca gccgatgaaa atttctgccg cgagcggacg gacggacaca ggggttcacg
     9601 cggaaaacca ggtcgttaat ttcaggacgg ccagtgaccg ggatccggga ttgatccgta
     9661 aaggacttaa cgcccatctt ccgccgcaca ttgtggtgaa gtcagtcgaa gaggtttcac
     9721 ccgatttcca ttcccgctac gacgcgaagt ccaagattta tgaatactgg gtctggaatg
     9781 ctcccttccg ttctcctctc ctgaacgctc gcgtttttca cgtcccgttc cacctgaatt
     9841 tgccggagat tcgaaaagcc gcgcggcgtc tggtcggccg tcacgatttc agctcgttca
     9901 cgtccggatc ggcgctctcc ggcggccccg ggaagaaaaa aggcggccgg gttcggacgg
     9961 taaaaaaaat aagcatccag aaaaaggggg atcttctgat tctccaggtc gaggcggacg
    10021 gcttcctcta tcatatggtt cgtaacattg tcgggaccct tcttgaagtc ggaaggggaa
    10081 agcggcggcc cgaagaaatg gcgaaaattc tccgggcgaa atcacgccga ttttccggaa
    10141 gtaccgcgcc cgccaaagga ttgaccctgc ggcaagttat ctattgagac ggcatattcc
    10201 attaaaaaat ccccctcatt aagcaggttg cttcccttga caaaagatta aaatatgtta
    10261 atgtttgcgc agaatttaag gtttttaaaa tacttttgaa agtaattttg caagtaataa
    10321 aaaaatagga ggtgtggcca tgaatgacct cacaaaaaaa attaagacgg gattaaaaaa
    10381 taatccggag agtaaaaaag acgaacttat acgtaaggcg gttgcggcaa ttacaacggc
    10441 tttttttatt ttcagcgggg ttcctgtgcc cacttccttt gccaattcgc atcaaacagt
    10501 catgaacgaa ttaaatcagc cggtatcaac cgtgacactt gaacaagcca atgcggcggc
    10561 cccggcgacg aggggttcca ttccgtccgg tgccgcgatt cccgccgaca atcctttatc
    10621 ttccccaacc cccgaatcag agcccgaaga tccggaattc cgggagacta tttatatcct
    10681 gccctcgtcc caggctgaaa gagtggcgga tatagattcc cttatggaat tgctcgcgtc
    10741 aaaagggatt acgagaatca cccgtgacca gattgaggcg ctgaagcccg cgcagacgga
    10801 taggatgcac atttggggag ccgcttacca tgtgtcacaa aatggcgatc aaatagcaat
    10861 agctttttca gcagtcaagg aatatgggga tacggttatg atcaaggggg ttgttccatc
    10921 atggcatcct tattctcgtt cctatttttc gaacattctc tatagtgctt ctcctgacag
    10981 cgatcccgtt gtgatcgata ggtccgcctg cgtagcccat atgggattta cggcagatgg
    11041 cctcctagcg attgagggag agactggtgg atcgtcagtc tacaacagtg aaggggaagt
    11101 cgtggctttt gccaaccatg ttgttgagct ttatcccgtt ccgagcggct ggacgcgggc
    11161 ggcgagtaac gcgaattacg cgttt
//
LOCUS       DAOSIO010000031        11124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_5794_length_11124_cov_4.567712, whole genome shotgun sequence.
ACCESSION   DAOSIO010000031 DAOSIO010000000
VERSION     DAOSIO010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 11124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11124
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5794_length_11124_cov_4.567712"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PK590_02125"
     CDS             complement(<1..593)
                     /locus_tag="PK590_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23428.1"
                     /translation="MLKADSGTRVKREFQLTTYYPAPHGDYKNINTTEDTRLATSSGN
                     VGIGTTSPHKDAKLEVNNTLRLTPVTGDAVAQKGGAEGAIRYSKDANGKDAGGLLYRN
                     ASGWMPLAGGGGTGGFKIVQDTFSTCCGECSVSVDCGTGYQLIRVGMGEGSGAATKAL
                     LGFSGAGSSVGPWSCYGSQTCKSPNDCKKDWAMIGLCA"
     gene            complement(860..1660)
                     /locus_tag="PK590_02130"
     CDS             complement(860..1660)
                     /locus_tag="PK590_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23429.1"
                     /translation="MLGQKLRVIKRVYRLRKIYFCFFIAVTVCLPHGYSSGLAICSKE
                     LHLVFTPLNYQDPSDFIKDREILVERLRKTVPFSEFPARLKIWDLGLAEKQIGEVFLP
                     TDVFPYVRVDQEFIKKVYERVGANYKLVLLNQTGSTDAAEFSTLEATSVIVLGRRHFN
                     TDFYFSSAFLHELGHSLGLRDETSKNDARACNPGYPNCAKSKEEAEIWWGKWVGQNSQ
                     VGFFKGCCGRAEYFKPTALSLMNDIYHSSSFGPVNEEYLRRVFLNCSQ"
     gene            complement(1773..2786)
                     /gene="tilS"
                     /locus_tag="PK590_02135"
     CDS             complement(1773..2786)
                     /gene="tilS"
                     /locus_tag="PK590_02135"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOG23430.1"
                     /translation="MKRNPMSDFLADFQNHIANQSFFRGKEKILIACSGGPDSIALLR
                     LMVALAPKHRWQLGVLHYNHRLRKSAGNDLRFVRFLSKELGLAFHSGSGNVRREAVKT
                     GTSIEEAARKMRYDFFLGTAKKLGIQKIALGHTLDDQAETVLMRILQGTGLRGLQGIR
                     EVHRMEGIDLIRPLLKFSKKEILDFCRTEKIEFRKDESNRSVRFFRNRIRLKLLPVLK
                     RDFNPRITEALARIPAIASREIALLDRLEAQAWKAAVKKVGTKRIELKREVFFQMESP
                     LQFRIIERALKKIHPQSGLSYEAWERIWRGFSRARNRWSLPKDIDFALTPKTVTVFKK
                     SSP"
     gene            complement(2803..2875)
                     /locus_tag="PK590_02140"
     tRNA            complement(2803..2875)
                     /locus_tag="PK590_02140"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2840..2842),aa:Met,seq:cat)
     gene            3034..4032
                     /locus_tag="PK590_02145"
     CDS             3034..4032
                     /locus_tag="PK590_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOG23431.1"
                     /translation="MASKIHPKYASRTRPPVEPRKFDTVLFDIDNVLVDTRRSYLEAI
                     RYTVEIFLTHGDVPFFSKSSSGKMPELLSTHDVEKFKMLGGFNDDWDCCYGILVYLLS
                     LPVTNRTIEALKKASNFDKLVKSVKERPLKVSGITAIYGRPSTLVIEKVARIFQEVYL
                     GKDIFPRLTHNKMRYWKKRGLIDREKPIFKSPLLQKIKDAGMKLGICTGRSSYEAAYA
                     LKAFGMDKLFDAITTMDDVKQEERLKKESLRKPHPFSLLKTTEKLGKKLHYLYVGDLP
                     DDVLAANRAKKYVSICSAAFPMMAADPFRAQKELEAAQPDYVLKSPSELFKILGIK"
     gene            complement(4108..5187)
                     /locus_tag="PK590_02150"
     CDS             complement(4108..5187)
                     /locus_tag="PK590_02150"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOG23432.1"
                     /translation="MKILQRYVLRELMLPCLLCFAVLCFIFMAGYLVKAADLIIGRGV
                     PFTQTLYVLLLVVPSLVSYTAPMSLLTGILIVFGGFSQHNEFRAMKASGVHPLRFMTP
                     PLILGFALSVFMFVFNDQVAPHARFELRRATKKMIMLHPMALIEPGRFVKISSDIIFL
                     TKRVEKNMMLDIVAHEGVDTDRPVRTIVAERGEIVTDPETGKLEVHLYDGSVSESVDK
                     GIQSVQFQTYRFPTLAENDVQKMGKKKKDMTLAEILVDVSEKTDPKDRRELWAVFHQR
                     IAFAFASLVFVFVGIPIATVVKRGEVVISFGISMAAASIYYVLFAIAQTLATRTGLPP
                     YVSLWIPNIVLLGVGTYLLRKAILS"
     gene            complement(5215..6453)
                     /locus_tag="PK590_02155"
     CDS             complement(5215..6453)
                     /locus_tag="PK590_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOG23433.1"
                     /translation="MLAVDKMMEYIAYFMLGLSLNLTPCVYPMLTITLSLFHGGRAES
                     HGQSFLKALAYVSGITVMFTSVGLLAALTGEFFGGLLQNFWVLFLMGLFIIALSLSMF
                     GLYTFQLPAWLMPQRRALREKTWFEFFLSGLLAGVVAAPCVGPVVLSLLTAVSQMGDA
                     VYGAVCFLILSIGMGFPYLILGTFSGLIRRLPKSGAWLVWFERLLAVVLFSFGCFYIL
                     IAFRWPIIHWLIPATLLGGGIYLGWLERSNRESRKFEIFKRVAGTLIAGVGLWMIASW
                     MWTYVDRGVVWEDYRQGVLDESREIGEPVVIDFYADWCIPCHDLHKSTFTNPGVIRAL
                     ERFRKVRADATRLDNEDTKNLIRLFGVYGVPTVIFLDEAGREVEEMRVSGFVGPKEFL
                     GMMRETGLLPSSDSEMLTNA"
     gene            complement(6484..7104)
                     /locus_tag="PK590_02160"
     CDS             complement(6484..7104)
                     /locus_tag="PK590_02160"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOG23434.1"
                     /translation="MIGKNILVMDLETQKSFKEVGKSKLDSLSKLKVSVVGVYDYLTD
                     QYRAYEEKELMDLDKRIQTADMLIGFNIRRFDMPVLQPYIFKSVDLLPVLDLLDDIEK
                     ARGHRASLDSVARATLKEGKSGDGAEALLLYKEGRLTELKKYCLDDVRLTRGVYEYGY
                     KEGKILFTSTWDYKTYEIPVNWRQSTEAFLSKKNTPGGNDFPTSLF"
     gene            complement(7128..7853)
                     /locus_tag="PK590_02165"
     CDS             complement(7128..7853)
                     /locus_tag="PK590_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOG23435.1"
                     /translation="MMTYSSKIPKEIRAVFLDAGGTLFRPYPSVGYHYRAVAARYGLV
                     ATEEDVEMAFRRVWEKHDAMGGLKGHVTEKNERDFWYKIVSEVFRGFGPLSDFEACFT
                     ELFDLFSRPTVWRFFPETEDVLRALKKKGYTLGLISNWDSRLVNLCEGFDLDRFMDFY
                     LISAIFGAAKPDPKIFRAALEKANVKAEESVHVGDSLEDDVRGAYQAGIRAIWLDRSG
                     RREKLAGHEREVLTVIRDLRELV"
     gene            complement(7837..8586)
                     /locus_tag="PK590_02170"
     CDS             complement(7837..8586)
                     /locus_tag="PK590_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG23436.1"
                     /translation="MSLTLGFPIPVFGSDPLPEDTLVAGTPSILELIAEEKAIKRKLG
                     EDPGDPALYFELSNVYASLFDITRKKKPESLKWLLKSGNALEKVILIDPGHKVARFNL
                     GIVYKRQGEMERAREEFKRVIRFSDPATDSRLISACWIQIGMIYSEQGFYDEAKDAFE
                     KALEFDYANHDIRDAIEEVKAAKLSGNFTEQSGIFSEQTMMRTRSSHDPYANEGWGTQ
                     PDSFGGVAQALPYLGQLLAQKFTQGGDDDLL"
     gene            complement(8612..8893)
                     /locus_tag="PK590_02175"
     CDS             complement(8612..8893)
                     /locus_tag="PK590_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23437.1"
                     /translation="MKTLSAHVLQVAEDGSSLTADFRHPATGKVYRLIFHMDSESGLS
                     GIKSLRNLRTGQVVSIDYVDGPAGKKIIRRIAKVKLSGPPPGLEKFNGL"
     gene            complement(8982..9497)
                     /gene="tpx"
                     /locus_tag="PK590_02180"
     CDS             complement(8982..9497)
                     /gene="tpx"
                     /locus_tag="PK590_02180"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019638819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOG23438.1"
                     /translation="MNTMERKNAVTFQGTPLTVVGPELKVGDAAPDFYVVDHALKTVT
                     LKSSQGQIRLVSAVPSIDTPVCELQTKRFNSEAAKLPADIVLMTVSMDLPFAQTRWCG
                     ATGIDRMQMVSDYQAASFGQAYGVLIKELRLLARSVFVIGPDDRIKYVEYVKEVTHHP
                     DYDKVLSFLKG"
     gene            complement(9547..9906)
                     /gene="queF"
                     /locus_tag="PK590_02185"
     CDS             complement(9547..9906)
                     /gene="queF"
                     /locus_tag="PK590_02185"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOG23439.1"
                     /translation="MAHPSKKIEVFKNPNSGRNYKIDLECPEFTCLCPKTGQPDFATL
                     RICYIPDKLCVELKSLKLYLWSYRDEGSFHEAVTNKILDDLVKAVKPRQMTLVADFFV
                     RGGIHTVITVEYFKDKK"
     gene            complement(9917..10510)
                     /gene="metW"
                     /locus_tag="PK590_02190"
     CDS             complement(9917..10510)
                     /gene="metW"
                     /locus_tag="PK590_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOG23440.1"
                     /translation="MSPYKNKLDHKVIYGLIESGAKVLDLGCGDGDLLSLLVRGKKIK
                     AQGVELSNDAIHACVEKGLSVFHSDIDSGLPYYPDQSFDYVILNQSLQEVKKVETVIE
                     ESLRVGRKVIVGFSNFAHFQERLSLFFGGKAPVSSSLPHEWYRTPNLRFLSVKDFREF
                     CAKKKYKILKSRFLGRKNLVWGLPNLFATNAIFVITK"
     gene            complement(10706..>11124)
                     /locus_tag="PK590_02195"
     CDS             complement(10706..>11124)
                     /locus_tag="PK590_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23441.1"
                     /translation="EIIEETAYPNLFLIPSREAKFSAPYALRSKRMKEMLNVLKETFD
                     VVIVDTPPVLVLNDAVAISELADIRVVVVEWGKTVIKSVLRTVSKIAPSDSALTGIIL
                     NKVKHWGKQYYYEHYYGARSGYFSKDVGTPKDVTPQ"
BASE COUNT         2998 a         3034 c         2460 g         2632 t
ORIGIN      
        1 gcacatagcc ctatcattgc ccagtctttt ttgcaatcat tagggctttt gcatgtctga
       61 ctcccgtagc acgaccatgg ccccacagag ctacctgccc cggagaaacc aaggagtgcc
      121 ttcgttgccg caccagaacc ttcccccata ccaacccgta tcagttggta gccagttccg
      181 caatcaactg aaacactgca ttcaccgcaa caagtagaaa aagtatcctg gacaatctta
      241 aaccctcccg tgccaccacc acccgccaag ggcatccagc cactggcatt tctataaagc
      301 aatccgccag catctttacc gtttgcgtcc ttactatacc gaatggcgcc ttccgctcct
      361 cccttttgtg ctactgcatc acccgtgaca ggtgtcagac gcagtgtatt gttaacttca
      421 agcttagcat ctttgtgagg agacgtggtc ccgatcccga catttcctga cgaggtcgcc
      481 agtcgggtat cttcggtcgt gttgatattt ttataatcac catgcggcgc ggggtagtaa
      541 gttgtcagtt gaaattctct cttgaccctg gtccctgagt ctgcctttag caccggtatt
      601 tgcataaaca gtagcgcaat cactccgcaa aacattattt gttttttatt cttcatgttc
      661 ttcctcccgt taaatttcaa tcattgcccg ccaacatgtc attataccgt tctctccctt
      721 cagctctcca taaaaaaatc cagggacggg aaaagaagct catctcttag ccgtgcgccg
      781 caccctccct gccacaagaa aatttctgct gtcttccggc atgtagcagt ttttattctt
      841 ccctcttgag ccggattgtc tattgtgagc aattaaggaa tactcgtctg agatattcct
      901 cattcacagg cccaaagctg gaagagtgat aaatatcatt catgagcgat aatgccgttg
      961 gcttgaaata ctctgcgcgc ccgcaacaac ctttaaaaaa tccgacttgt gaattttgcc
     1021 cgacccactt accccaccat atttcagctt cttcctttga tttcgcacaa ttcggatatc
     1081 ccggattaca tgcacgagca tcattctttg aagtttcatc ccgaaggccc agcgaatgcc
     1141 ctaattcatg aaggaaggcg cttgaaaaat aaaaatccgt attgaaatgc cggcgaccca
     1201 gcacaatcac cgaggttgct tccagagttg aaaattccgc agcatccgtg gatcctgtct
     1261 ggtttaaaag aaccagttta tagttggcgc cgacccgttc ataaactttt tttatgaact
     1321 cttgatctac gcgcacgtag ggaaacacat ccgtcgggag aaaaacttcc ccgatttgct
     1381 tttcagccaa tccaagatcc caaatcttca atctcgctgg aaattcactg aaagggactg
     1441 ttttccgcaa tcgttcaacc agaatctccc ggtcttttat aaaatccgaa ggatcctgat
     1501 agttcagagg agtaaaaacc agatgaagtt cttttgaaca aatggccaaa cccgaagaat
     1561 atccatgggg aaggcaaact gtcaccgcaa taaaaaaaca aaaataaatt ttcctaagtc
     1621 tataaacccg tttaatcacc ctcagctttt gccccagcat acctcacagc tttagttttt
     1681 tagattctct aaacatccgg acatcccggt tccttttcaa tttcctgacg ttgttaaaag
     1741 tcaaaataac ctgttccctt cacgtccgga attcacgggg aagatttctt gaaaacagtg
     1801 accgttttcg gagtgagggc aaaatcaatg tccttcggaa gggaccaacg gttacgggca
     1861 cgggaaaacc ccctccagat ccgttcccag gcttcatagg aaagcccgct ctgcggatga
     1921 atttttttga gagcccgttc gatgatccga aactgtaggg gcgattccat ctgaaaaaag
     1981 acctcccgct tcaattcaat acgtttcgtc cccacctttt ttacggcggc cttccaagct
     2041 tgcgcttcca gtcggtccag aagcgcaatt tcccgggaag caatcgccgg aatccgggcc
     2101 aatgcctccg tgatccgggg attaaaatcc cgcttgagga ccggcaataa cttcaggcgt
     2161 atccggttcc ggaaaaaccg gacggaacgg ttactttcat ccttgcgaaa ctcaattttt
     2221 tctgtacggc aaaaatcaag gatttccttt ttggaaaact tgagaagagg gcggatcagg
     2281 tcaatcccct ccatccggtg gacctcgcga atcccctgga gtccccgaag gcctgtcccc
     2341 tgcaaaatcc tcatcaggac agtttccgcc tgatcatcaa gggtatgtcc cagggcgatt
     2401 ttttgaattc caagtttctt cgccgtgccg agaaaaaaat cataccgcat ctttctggcg
     2461 gcttcttcga tggaagttcc ggtcttgaca gcctctcgtc tgacgttacc ggatccggaa
     2521 tgaaacgcaa gccccagttc cttcgaaaga aaccgaacaa agcgaagatc gttccctgcc
     2581 gatttccgga gacgatggtt gtaatgaagg acgcccagtt gccagcgatg tttcggggcc
     2641 agggcaacca tcagtctgag aagcgcgatc gaatcggggc caccggaaca ggcgatgagg
     2701 attttttctt ttccccggaa aaaagactga ttcgcgatgt ggttttgaaa atccgcgagg
     2761 aaatcactca tggggttccg tttcatggct tcaaaaagac attgccggag ggcgggatcg
     2821 aaccgccgac acgaggctta tgagtcctct gctctaccaa ctgagctacc ccggcaagac
     2881 agatcactga caagggaaat aaaaccgcca catccttctc aagcggtggc gggtatacta
     2941 gcacaggcgc cttcattcgc caagagcggc ccgtttcaaa aaattaatga gaagtttctt
     3001 tacttaccga aaagttgggc gtaaacttgg ttcatggctt ccaagattca tcccaaatac
     3061 gcatctcgta ctcgtcctcc ggttgagccg cgcaagttcg atactgttct tttcgatatc
     3121 gacaatgtcc ttgtcgacac gcgccgctct tacctggaag ctattcggta taccgtggag
     3181 atcttcctca cccacggcga cgtccctttt ttcagtaaat cttccagcgg taaaatgccc
     3241 gagcttttgt cgactcatga cgtggaaaaa ttcaagatgc tcggcggatt taacgatgac
     3301 tgggattgct gttacggaat actggtctat ctcctgtcac ttccagttac caaccggacg
     3361 attgaggctt taaaaaaagc ttccaatttc gacaaactcg taaaatcagt caaggaacga
     3421 cctctgaagg tttctggcat taccgcgatt tacggccggc caagcaccct cgtgattgaa
     3481 aaagtggcaa gaatcttcca ggaagtctac ctgggcaagg acattttccc ccgcttgacc
     3541 cataacaaga tgagatactg gaaaaaacgc ggactgatcg accgggaaaa acctattttc
     3601 aaaagccccc tcctccagaa aatcaaggat gccggaatga aactcggaat ttgcacgggc
     3661 cgatccagct acgaagcagc ctacgcgttg aaagccttcg ggatggacaa actttttgat
     3721 gccataacga ccatggatga cgtcaaacag gaagagcgcc tcaaaaagga gtccttaaga
     3781 aaacctcatc cgttttcgct cctcaaaacc acggagaaac tggggaaaaa acttcactac
     3841 ctttatgtcg gcgacctgcc tgacgatgtc ttggccgcaa atcgcgcgaa aaaatacgtt
     3901 tcgatttgtt ccgccgcctt ccccatgatg gcggccgatc cgtttcgcgc ccaaaaagaa
     3961 cttgaagccg cccaacccga ttacgtcctg aagtctcctt ccgaactttt caaaattctc
     4021 gggatcaagt aactcccttc caaaatttcc gacgggagat tcctttttag aaacgaaatt
     4081 accagaaatt cgggaaactg aaaattttca ggagaggatt gctttccgaa gaaggtaagt
     4141 ccctacccca agtaatacga tgttcgggat ccagaggctc acgtaaggtg ggaggccggt
     4201 acgggtcgcg agcgtttgag cgatggcaaa aagcacgtaa taaatacttg ccgcggccat
     4261 cgagatgccg aaactgatca ccacttcacc gcgtttcacg acggtcgcga tgggaatccc
     4321 aacaaaaacg aagacaagcg acgcgaaggc gaatgcgatg cgctggtgaa acacggccca
     4381 aagttcacgc cgatccttgg gatccgtctt ctcggaaaca tcaacgagaa tctcggcaag
     4441 ggtcatgtcc ttttttttct ttcccatttt ctggacatca ttctcagcca aagtcgggaa
     4501 cctgtaggtc tggaattgaa ccgactggat ccccttgtcc acagattccg aaacgcttcc
     4561 gtcataaaga tggacctcaa gttttcccgt ttccggatcg gtcacgatct ccccccgttc
     4621 cgccacaatg gttcgcaccg gacgatcagt gtcgacgcct tcatgggcca ctatatccag
     4681 catcatgttt ttttccacgc gttttgtcag aaagatgata tcgctcgaaa tcttcacgaa
     4741 ccggccgggt tcgatcaacg ccatgggatg gagcataatc atcttcttgg tcgcccgcct
     4801 gagttcgaac cgggcgtgag gcgccacctg atcgttgaag acaaacataa aaacactcaa
     4861 cgcaaatccg aggataagcg gcggtgtcat aaacctgagg gggtgaacac ccgaggcctt
     4921 catggcccgg aattcattat gctggctgaa accaccaaac acgataagga ttcccgtcaa
     4981 aaggctcatc ggcgccgtgt aactcaccag ggaagggaca acaagcaaca gaacataaag
     5041 cgtctgagta aacgggacgc cccgtccgat gatcaggtca gccgccttga ctagataccc
     5101 cgccataaaa ataaaacaaa gcacggcaaa acacaaaaga cagggaagca ttagctctct
     5161 gagaacatat cgctggagaa ttttcatagg cgtctcgagg ataataggag ggggtcaggc
     5221 atttgtcaac atttcggaat cagaagatgg taacaacccg gtttcacgca tcatacccag
     5281 aaactccttc gggccgacaa aaccggagac ccgcatttcc tcgacctccc ggcccgcttc
     5341 atcaagaaaa ataaccgtcg gaaccccata aaccccaaaa agacggatca gatttttcgt
     5401 gtcttcatta tcaagacggg ttgcatcggc cctcaccttc ctaaaccgtt ccagggcacg
     5461 gatcacaccg ggattcgtaa acgtggattt atggaggtcg tggcaaggga tacaccagtc
     5521 cgcgtaaaaa tcaatcacaa ccggctcacc aatctctcgt gattcgtcca agacaccctg
     5581 tctataatct tcccagacca ctccgcggtc aacgtaagtc cacatccagc tcgcgatcat
     5641 ccacaaaccg actcccgcga tcaaagtacc ggccacgcgc ttaaaaattt caaactttct
     5701 tgattcccgg ttcgaccgct ctaaccagcc gagataaata ccacccccga ggagcgtcgc
     5761 gggaatcagc caatgaatga tcggccatcg aaacgcgatg aggatataaa aacaaccaaa
     5821 actgaaaagg acgaccgcca gtagccgctc aaaccagacc agccaggcgc cggatttggg
     5881 aaggcgacgg atcaatcctg aaaaagtccc gaggataaga taagggaacc ccatcccgat
     5941 cgacaggatc aaaaaacaaa ccgcgccata aacggcatcg cccatctgac tgacggccgt
     6001 caaaaggctc aggaccacag gacctacgca gggagcggcc accacccccg cgaggagtcc
     6061 cgaaagaaaa aattcgaacc aggtcttttc cctcaatgcc cgccgctgag gcatcagcca
     6121 ggcaggcagt tggaaggtat ataggccaaa catgctcaag gacagggcga tgataaaaag
     6181 ccccatcaaa aaaagaaccc agaaattctg aagaaggcct ccaaaaaact cgcccgtcag
     6241 cgcggccaga agaccgactg acgtaaacat caccgtaatt cccgaaacat acgccagggc
     6301 cttcaggaaa ctctgcccgt ggctttcggc gcggcctccg tgaaaaagcg atagcgtgat
     6361 cgtcagcatt gggtaaacgc acggtgtcaa attcaggctt aatccgagca taaaataggc
     6421 aatgtattcc atcatcttat ccaccgcgag cattcactcc agcatcgttt gaaagcgaat
     6481 ccattaaaac aggctggtgg gaaaatcgtt tcctcccgga gtattctttt ttgaaagaaa
     6541 cgcttcggtg gactgtctcc agttcaccgg aatttcgtag gttttataat cccaggtcga
     6601 cgtaaaaaga attttccctt ccttgtatcc gtactcataa acacccctgg tgaggcgaac
     6661 gtcatccaga caatattttt taagctcggt caaacgcccc tctttataaa ggagcagagc
     6721 ttcggctccg tcgccggact ttccttcttt caaagtcgca cgcgccacgc tatccagact
     6781 ggcgcgatgc ccacgcgctt tttcgatatc atcaagcaga tccagcacgg gtaacaaatc
     6841 gaccgattta aaaatgtagg gttgaagcac gggcatgtca aaccgccgga tattaaaccc
     6901 gatcagcatg tccgccgtct ggatccgctt gtcgagatcc atcaattcct tttcttcgta
     6961 ggcgcgatat tggtctgtca gataatcgta aacccccacg accgaaacct tgagtttcga
     7021 aagactgtca agcttcgact tcccgacttc cttgaacgac ttttgtgtct caagatccat
     7081 gacaagaatg tttttcccta tcatcttccc tttcctctcg cgatcctcta gaccaattcc
     7141 cttaaatcgc gtatcaccgt cagaacttcc cgttcatgcc ccgcaagttt ctcacgacga
     7201 ccggaacgat caagccagat cgcccggata cccgcctgat aagctccacg aacgtcgtct
     7261 tctaggctgt cccccacatg caccgattct tcggctttca cgttcgcttt ttcgagcgcg
     7321 gctcgaaaaa ttttcggatc cggcttcgcg gctccaaaaa tcgcgctaat caggtaaaaa
     7381 tccatgaacc ggtcgaggtc aaacccttcg caaagattga cgagccggct gtcccaattt
     7441 gaaataagtc cgagagtata ccctttcttt tttagagcgc gcaagacatc ttcggtttcg
     7501 gggaaaaatc gccagacggt cggccgcgaa aaaagatcaa aaagttccgt gaagcaggct
     7561 tcaaaatccg aaagaggccc gaacccccgg aaaacctcgg aaacaatttt ataccagaag
     7621 tcccgctcgt ttttttcggt cacatgcccc ttcaagccac ccatcgcatc gtgcttctcc
     7681 cagacccggc gaaacgccat ttcaacgtct tcttcggtcg ctaccaggcc atacctggcc
     7741 gctacggccc ggtaatgata acccaccgaa ggatatggcc ggaaaagagt ccctccggcg
     7801 tcaagaaaaa cggcgcggat ctctttggga attttactag agtaagtcat catcccctcc
     7861 ctgggtgaat ttctgcgcga gaagctggcc caaatacgga agcgcctgag ccactcctcc
     7921 gaaagagtcc ggctgagttc cccagccctc attagcgtag ggatcgtgcg aagatctcgt
     7981 ccgcatcatt gtctgctcgg aaaatattcc ggattgttcg gtaaaatttc ccgacaattt
     8041 tgccgcttta acttcctcga tcgcgtccct gatgtcgtgg tttgcgtagt caaactccag
     8101 ggctttttca aaagcgtctt ttgcttcatc ataaaacccc tgttcagaat aaatcatccc
     8161 aatctggatc cagcaggctg aaatcaatcg cgaatcggtg gccggatctg aaaaccggat
     8221 caccctttta aactcttctc gagcccgctc catttctccc tgacgtttat agacgattcc
     8281 gagattgaac cgggcgacct tgtgaccagg gtcaatcagg ataaccttct caagggcatt
     8341 gccgcttttt aaaagccatt tcaaagattc cggctttttt ttacgagtga tgtcaaaaag
     8401 cgacgcataa acattggaaa gttcaaaata aagggctgga tcccccggat cctcgcccaa
     8461 ctttctttta atcgctttct cctccgcgat cagttcaagg atcgacgggg tcccggcaac
     8521 taaagtatct tcggggagag gatcggagcc gaaaacaggg atcggaaatc ccagtgtcag
     8581 actcaagaga aggaagcaca acaagttttg cctaaagacc attgaacttt tcgagccccg
     8641 gtggcggtcc cgaaagcttc acttttgcaa ttcgccggat gatttttttg ccggccggac
     8701 cgtccacata gtcaatcgaa accacctgtc ctgtccgaag atttctgaga ctttttatac
     8761 cgcttaatcc ggattctgaa tccatatgaa aaatcaaccg gtaaaccttg ccggtggccg
     8821 ggtgacgaaa atcggccgtt aaacttgatc catcctccgc gacctgaagc acgtgcgctg
     8881 aaagagtttt catcacgggc ggattcgccc aaacgttgga agagaaaaca acaccgaccg
     8941 tggtgagtat gatccaagat gcgaaacgcg acataagaag atcaaccttt taaaaaactt
     9001 aacaccttat cgtagtccgg atgatgggtc acctctttca catattccac gtatttgatc
     9061 ctgtcatccg ggccaatcac aaaaactgaa cgcgccaaaa gccgcaactc cttgattaaa
     9121 acaccgtacg cctggccaaa agacgccgcc tggtaatcgg acaccatctg catcctgtca
     9181 attcccgtgg cgccacacca ccttgtctga gcgaaaggaa gatccatgct gacggtcata
     9241 agaacgatat cggcgggaag tttggccgct tcggaattga aacgtttggt ctggagttcg
     9301 caaacgggag tgtcaatcga agggacggca ctgaccagac gtatttgccc ttgagacgat
     9361 ttcagagtca ctgttttcag ggcatgatca accacataaa aatccggcgc cgcatccccc
     9421 actttaagtt ccggccccac caccgtgagc ggggttccct gaaaagtgac cgcgttttta
     9481 cgctccatgg tattcatcgc acactccagc gctgtaaggt ttttgtcgtg atcagatgtt
     9541 gaaaatttat ttcttatctt tgaaatattc gaccgtaata accgtgtgaa taccaccccg
     9601 aacaaaaaaa tccgccacaa gggtcatttg ccggggcttc actgccttca caaggtcatc
     9661 caggatcttg ttggtcacgg cttcgtgaaa cgagccttcg tcgcgatagc tccaaaggta
     9721 cagcttcaga cttttgagtt cgacgcataa cttatccgga atatagcaaa tccggagggt
     9781 tgcaaaatca ggctgtccgg ttttcgggca aaggcacgta aactccggac attcaagatc
     9841 aattttgtaa tttcgccccg aattcggatt tttaaaaact tctatttttt ttgacggatg
     9901 cgccacggcc aaaaacctat tttgtgatca caaaaatcgc gtttgtcgcg aaaaggttgg
     9961 ggagccccca gacgagattc tttctgccca aaaaacgaga cttgaggatc ttgtattttt
    10021 ttttcgcgca aaactccctg aaatctttaa cggaaaggaa ccgtaaattg ggcgtccggt
    10081 accactcatg cggaagcgag gagctgacgg gcgccttgcc tccaaaaaag agagataacc
    10141 gctcctggaa atgagcgaag ttggaaaaac caacaatgac ctttctgcca acgcggagcg
    10201 actcttcgat caccgtttca acttttttca cttcctgcaa actctggttc aatatgacat
    10261 aatcaaacga ctgatcagga taataaggca ggccgctgtc gatatcgctg tgaaaaacac
    10321 tcagcccctt ctcgacacaa gcatgaatag cgtcattcga cagttcaact ccctgggcct
    10381 tgatcttctt cccgcgaaca agaagactaa gcaaatctcc gtcgccgcaa ccaagatcaa
    10441 gcaccttggc cccggattcg ataagaccat aaataacctt gtgatcgagt ttatttttat
    10501 agggtgacat gtcccctcca gaggaagatg cgatcgtaag cttcccgcca agatttttca
    10561 agaataaact gggagaataa gcaaatcagc ggctaacgta aaaaactcag ggaagagcca
    10621 aaaaccaaac gaagcgcgaa ccgattcgtt tttagatagt ggcctccgat atcactcgcc
    10681 tgagatccta gagctcagaa cgtttctatt gaggcgtaac gtctttcggc gtgccaacat
    10741 ccttggagaa atacccgctc ctggcaccgt aatagtgctc ataataatat tgtttgcccc
    10801 aatgtttgac cttgttgaga ataatgccgg tcaaagccga gtccgagggg gcgattttgc
    10861 taacggttcg aagtactgat ttgatcacag tctttcccca ctccacgacc accacacgaa
    10921 tatcagccaa ttcgcttatg gcaacggcgt cgttaagcac cagcacgggc ggagtatcta
    10981 caataacgac gtcaaaagtt tccttaagta cattcaacat ctctttcatg cgttttgacc
    11041 gaagcgcgta aggggcgctg aatttggcct cccgggacgg gatcagaaaa agattgggat
    11101 aggcggtttc ttctataatc tcgt
//
LOCUS       DAOSIO010000032        11121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_5796_length_11121_cov_4.086933, whole genome shotgun sequence.
ACCESSION   DAOSIO010000032 DAOSIO010000000
VERSION     DAOSIO010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 11121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11121
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5796_length_11121_cov_4.086933"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2217
                     /gene="ppdK"
                     /locus_tag="PK590_02200"
     CDS             <1..2217
                     /gene="ppdK"
                     /locus_tag="PK590_02200"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014094464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOG23442.1"
                     /translation="FIQMFGDVVMEVPHDEFEHVLEKKKQQVGAKADTDLNANDLKEV
                     VRQYKKVYQDHTGEEFPQDPFDQLVKSVNAVFNSWNCPRALKYRQINEIRGLLGTAVN
                     VQAMVFGNMGETSGTGVCFTRNPSTGENKFYGEYLVNAQGEDVVAGIRTPEPISVLAQ
                     KWPAVYKQLDTIRLRLERHYRDMQDIEFTIQEGKLYILQTRSGKRTAAAAVRTAVEMV
                     KEKMISQKDAILRVAPKQLDQLLHPTFDPKQQKTKQPIATGLPASPGAATGRVVFTAD
                     EAEAWVERGETVVLVRVETSPEDIGGMNVAVGILTSRGGMTSHAAVVARGMGKCCVAG
                     VSGINIDYKAKRFTAGKVTVKEGDFISLDGTLGYVYEGKLNTIEPTLSGDFGTLMKWA
                     DGVRVLKVRTNGDTPLDATVARKFGAEGIGLCRTEHMFFEGDRIIAVREMILADDTAG
                     REKALAKLLPMQRGDFEGIFEAMKGLPVTIRLLDPPLHEFLPQEEENQKEMAQVMNVP
                     VDVIKNKVNALHEFNPMLGHRGCRLGITYPEIYAMQVRAIIEAACNLKKKGIKVIPEI
                     MIPLIGTKKEFDILKELSIGVINKVFEEKKTKVDYMIGTMIEVPRAALTADRVAEAAD
                     FFSFGTNDLTQMTLGFSRDDSGNFMGAYLEQNIFEKDVFQSIDEEGVGSLVRLAVEKG
                     RKTKKDLKIGICGEHGGDPDSVDFCHRVGMNYVSCSPYRVPIARLAAAQAALRGKK"
     gene            2319..3503
                     /locus_tag="PK590_02205"
     CDS             2319..3503
                     /locus_tag="PK590_02205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOG23443.1"
                     /translation="MAGKNKKKKTGSVRQFKKRRVQVKARRRRSKRSRLVVRRSHLGS
                     KLSSSKSSRSSSKSKTEKLLLKEKAFQEEASNLVRKEALLETTGERRKQLREEDEPDD
                     NHSEDSTDQTLDRTAMKIYLDQIEHIPLLTPEEEISLAKLVQRGGPKGEEARHRMIRS
                     NLRLVIAIAKRYTHMGLPFSDLVEEGNIGLMRAVEKFDPKRGYRFSTYASWWIKQGMM
                     RSLSNHGKTIRIPVYMYDIIARWRRVRDALTQRLNRPPTRREIAKVLEIPVAKVREIE
                     NIANKPSSLNTPISLDGTAELIDLIEDDVANNPDSRAAEVLKSERIQNLLDHLDERER
                     KILTLRFGFGEHDPHTLEEVAQHFNVTRERIRQIEAVALKRIRARLVLEGDIFENYLT
                     HQ"
     gene            3542..4063
                     /locus_tag="PK590_02210"
     CDS             3542..4063
                     /locus_tag="PK590_02210"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOG23444.1"
                     /translation="MSVTSLRSKVRDVADFPKKGILFKDITPVLRDPAAFREVIALLA
                     KAVKKSKADLVAGIESRGFLFGAPVATKLGIGFVPIRKKGKLPWRTKRVRCTLEYGQD
                     VLEIHADAVKRGQKVAIVDDLLATGGTAQAAAKLVRLIGGKVVLQAFVIELGFLNGRK
                     KLAKCDVSSVLKF"
     gene            complement(4132..4205)
                     /locus_tag="PK590_02215"
     tRNA            complement(4132..4205)
                     /locus_tag="PK590_02215"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4169..4171),aa:Arg,seq:cct)
     gene            4222..4860
                     /locus_tag="PK590_02220"
     CDS             4222..4860
                     /locus_tag="PK590_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23445.1"
                     /translation="MGIDLKIQNLKNALSFIFVLGAIFIFVTPAFSDEASDLNVQPET
                     MAEFETLKSEWEKVRDQQIRMIQDKEQDLERLKEELFQKMRDGNPQNFSGDYAGTPPT
                     LGEDDRLAAAAADPRVIESFEQKIAELKEANRKLSGEISELKARNRELEMVAGGSLAS
                     DSGRDELRKDKEELDIQKAAFQREREKFFQEMARQRERLRKAELDQAGGGLN"
     gene            4891..5418
                     /locus_tag="PK590_02225"
     CDS             4891..5418
                     /locus_tag="PK590_02225"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOG23446.1"
                     /translation="MRRELLARRQAIPRSKRLQKSRKILRKLFKHPRFLAAKKIAFYF
                     DIAPEVQTRLCLETLVKDKEIYLPRVNLNTRRLSLCRIQSIKNDLQRGAYSIMEPKAT
                     CPRRSVSRMEVIVVPGVGFDKKGNRMGRGAGYYDQLLRKAGKIFKIGLCFREQMVRQL
                     PVTKTDVPMDCVITD"
     gene            5480..6703
                     /gene="xseA"
                     /locus_tag="PK590_02230"
     CDS             5480..6703
                     /gene="xseA"
                     /locus_tag="PK590_02230"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015734457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOG23447.1"
                     /translation="MKILDGKIYTISELNKEVRGVLETRYTGIWVEGEISNFKRHSSG
                     HIYLTLKDEQSQISAVFFSFYHKGLKFEPEDGHKVLVFGRVSVYEARGQYQFYIERIE
                     PKGVGALQLAFQQLKERLEKEGLFRSERKRPVPKFPKTVGVITSPTGAAIHDILNVIN
                     RRFCGTHILLYPVKVQGEGAGEEIAEAIRIMNQRGEAEVLIVGRGGGSLEDLWAFNEE
                     PVARAIFDSAIPVISAVGHETDWTIADFVADLRAPTPSAAAELVVASRGELELKIKDA
                     AERLRQGMIFRLRERRETLKNLAASAAFRQPRYLLEQFIERMDELGKRLQTSLTNWMT
                     FKKQVFQTATGKLNVLNPLGILERGYSVTFDARGAVLKSAENLPTGTEIRTRLHQGEL
                     VSRVIATKETCSPRT"
     gene            6738..7043
                     /locus_tag="PK590_02235"
     CDS             6738..7043
                     /locus_tag="PK590_02235"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOG23448.1"
                     /translation="MAEKKEKIDFEKAMESLEKTVAALEGGELTLDQALKKYEEGVGL
                     VRACQSQLTEAEKKIEVLTRALDGSFKKEPFDACGKPAAKVRKKGLVRGENSSDEED"
     gene            7044..7871
                     /locus_tag="PK590_02240"
     CDS             7044..7871
                     /locus_tag="PK590_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOG23449.1"
                     /translation="MADYGTCFKVHLRLIEQTLKKELQSRAGRPKAIHQAMQYAVFTG
                     GKRFRPILVLAACEACGGRMKDAIRPACALELIHTYSLVHDDLPALDNDAVRRGRLTC
                     HKKFGEATAILAGDGLLTLAFEILSGVQPAERAIRLVREISIAAGARGMIAGQVEDIE
                     TASRRLDLATHDFICANKTGKLIYASVRAGALVADASPKQLVLVDRYARAVGLAFQVV
                     DDLLDQDGYCRFMPEDRVVQKAGQLIKTAKKVAGKLGAKARCLVLLADFLESRIPQK"
     gene            7887..9818
                     /gene="dxs"
                     /locus_tag="PK590_02245"
     CDS             7887..9818
                     /gene="dxs"
                     /locus_tag="PK590_02245"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOG23450.1"
                     /translation="MTNWIHKINSPDDLKKVPLEKLPEVAEEYRQFLIESVAKTGGHL
                     GASLGTLELTMALHYVLNSPTDKICWDVGHQAYVHKMITGRRDQMATLRQPGGLSGFP
                     APWESCHDPFIVGHAGTAISQAIGLACARDIRKGNEQVVAIVGDGALTAGLSFEALNH
                     AGQLKKNMLIILNDNEMSIAKNVGAVSRYLNKVLTHPLYNKVRGEMEKQLQKFPRLQK
                     LANTSLDSMKHLFVPGVLFEEFGFRYFGPVDGHNVIHLVKILKDILALNTCSLLHVIT
                     KKGRGCEYAEQDQEKGHGVAPFNVKTGVKMEGARETNSTHGISFTQAFSDAILKHARR
                     DPSVVAVTAAMLSGTGLTDFQKVFPERCFDVGIAEQHAVTFAGAMARSGLKPVVAVYS
                     TFMQRSHDSIMHDVALQRTGVVLALDRAGIVGADGATHQGIFDMAYLGSVPGIVIGAP
                     KDEFEMEKMLELGLEASVPFVLRYPRANIAKLFDEFPKRSFQTGEGEVMFDGEDVTLL
                     ALGSMVEVACKAHALLREKGVSARVVNMRFAKPLDETLILESIRKTRMLFTLEEHVIT
                     GGFGAKVLEFLEIRNLRTVALKRLALPDEFIEHGSREKQLDSFGLSPEKVADSVIKEL
                     SGQKKCEKSAGFGIEPNPR"
     gene            9835..10098
                     /locus_tag="PK590_02250"
     CDS             9835..10098
                     /locus_tag="PK590_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23451.1"
                     /translation="MEPNEEIKQGDAGVAGETKEAKFKRLAEQRVAAALDKIRLIGNL
                     ASSQYAYNGEQVAKIFSALKGSIEDIEDKFHRTLERKKETFHL"
     gene            10205..11044
                     /locus_tag="PK590_02255"
     CDS             10205..11044
                     /locus_tag="PK590_02255"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOG23452.1"
                     /translation="MIKKIGIVVNQKKRGAIELAAKIRDWLEKRDLVVVDSLTEPIEQ
                     FIEDTDFVISLGGDGTMLSIVKHLRNRSVPVLGINLGGLGFLTEVKQSEVFEALAAYF
                     QIPEIEDRIMLSCTAWSKTKKMERRFLALNDIVISREGLARILRLDVFVSGEKLTSFR
                     GDGVIIATPTGSTGYSLSADGAVVHPKLEVFIITPICPHALSLRSLVIGADEKIMVQV
                     RTDDPAEKALLTADGQEKVEIDDSYIVEIGRSNAPFKLIKSTKRSYLETLREKFKLPD
                     TEA"
BASE COUNT         2811 a         2536 c         3273 g         2501 t
ORIGIN      
        1 tttatccaga tgttcggcga tgtcgtgatg gaagtgccgc acgatgagtt cgagcatgtg
       61 cttgaaaaga aaaaacagca ggttggcgcg aaggctgata ccgatctgaa cgccaatgac
      121 ctgaaagagg ttgtccgtca gtataaaaaa gtctatcagg atcacacggg agaagagttt
      181 ccccaggatc cgtttgacca gttggtgaaa tccgtgaacg cggtgtttaa ttcctggaat
      241 tgcccgcgcg cgctcaagta tcgccagatc aacgaaatcc gcggtttgct tggcacggcg
      301 gtgaacgtcc aggcgatggt gtttggcaac atgggggaaa cgtccggaac gggtgtctgt
      361 tttacccgta acccttcgac cggcgagaat aagttttacg gcgagtatct tgtgaacgcg
      421 cagggcgagg acgttgtggc cgggatccgg accccggaac cgatttcggt actcgcgcaa
      481 aaatggccgg cggtatacaa gcaactcgat actatccggt tgcgtcttga gcggcattac
      541 cgggacatgc aggatattga atttaccatt caggaaggga aactttacat ccttcagacc
      601 cgtagcggaa aacgcacggc ggccgcggcg gtccgtaccg cggtggaaat ggtgaaggaa
      661 aaaatgatct cccagaagga cgcgatcctt cgggtcgctc ccaaacagct ggatcaattg
      721 ctccatccga cgtttgatcc caaacagcaa aaaacaaaac aaccgatcgc gacgggtctt
      781 ccggcttctc cgggcgccgc aaccggcaga gtggtgttta ccgctgatga ggcggaagcg
      841 tgggtggaga gaggggagac cgtggttctc gtccgtgttg aaacttcgcc cgaagatatt
      901 ggcgggatga acgtcgcggt cggtattttg acctcacgcg gcggcatgac gtcgcacgcg
      961 gcggtggtcg cccgcggtat ggggaaatgc tgtgtggcgg gagtgagcgg cattaacatt
     1021 gattataagg cgaagcggtt caccgcgggg aaagtgaccg tgaaggaagg ggattttatt
     1081 tccctcgacg gaacgcttgg ctacgtttac gaaggtaaac tcaataccat tgagccgacg
     1141 ctttccgggg acttcggaac tcttatgaag tgggccgatg gtgttcgcgt tctcaaggtg
     1201 cgaacgaatg gcgacacgcc gcttgacgcg accgtggccc gcaagtttgg tgccgagggg
     1261 attggtcttt gccggacgga acatatgttt tttgagggag atcggatcat cgccgttcgc
     1321 gaaatgattt tggccgatga cacggcggga cgcgagaagg cgcttgccaa acttctcccg
     1381 atgcaacgcg gagattttga aggtattttt gaggcgatga aggggcttcc cgtgacgatc
     1441 cgtttgctgg atccgcctct tcatgagttc ctcccgcagg aagaagaaaa ccagaaagaa
     1501 atggcgcaag tcatgaatgt gccggtggat gtcatcaaga acaaagtcaa tgccctgcac
     1561 gagttcaatc cgatgttggg gcatcgcggc tgtcgtcttg ggatcacata tccggagatt
     1621 tatgccatgc aggttcgcgc gatcattgag gcggcttgca acctcaagaa aaagggaatt
     1681 aaagtgattc ccgagatcat gatccccctt atcgggacca aaaaggaatt tgacattctg
     1741 aaagagcttt cgatcggggt gatcaacaag gtttttgagg aaaagaagac caaggttgat
     1801 tatatgatcg gaacaatgat tgaagttccg cgggcggcgt tgaccgcgga tcgtgtcgcc
     1861 gaggcggcgg attttttctc gttcgggacg aacgatctga cgcagatgac cctcgggttt
     1921 tcacgcgatg attccggaaa cttcatgggt gcgtatctcg agcagaatat tttcgagaaa
     1981 gacgtgtttc agtccattga tgaagaagga gtcggctccc tggttcgtct ggcggtcgaa
     2041 aagggtcgta aaacaaagaa ggacctaaaa atcggaattt gtggtgagca tggcggagat
     2101 ccggattcgg ttgatttttg tcaccgggtc gggatgaatt atgtcagttg ctcgccgtat
     2161 cgtgtgccga tcgcgcggct cgcggcggcc caggcggcgc ttcgaggaaa gaaataagcg
     2221 ccaacgttta aaaaccattg ctccttcgga aggtcagggg aaactctggc cttccgaaga
     2281 taacgccggc aggcgagaga tatcacctaa cggttctcat ggcaggaaaa aataaaaaaa
     2341 agaaaaccgg ctctgttcgc caatttaaaa aacggcgtgt tcaggtaaaa gcaaggcggc
     2401 gtcgctctaa gcggtcccgg cttgtggttc gccgttccca cctcggttcc aagctgtctt
     2461 catccaaaag ctcgcgttcc tccagtaaaa gcaaaacgga aaaactcctt ctgaaagaaa
     2521 aagcgtttca ggaggaagct tcgaatctcg tcaggaagga agccctgctt gagacgacgg
     2581 gggagcgtag gaagcaactt cgcgaagagg atgagcctga cgataaccat tccgaagatt
     2641 cgaccgatca aacgctcgat cgaaccgcga tgaaaattta tctggatcaa attgagcaca
     2701 tccctctttt gacgccggag gaggaaatat cgctggcgaa actcgtgcaa aggggcggcc
     2761 ccaaaggaga agaagcccgt caccgtatga tccgttcgaa tctccgtctc gtgattgcca
     2821 ttgccaaacg ctacacgcac atgggacttc ccttttcaga tctggtggaa gaagggaata
     2881 ttggcttgat gcgcgcggtc gagaagtttg atccgaagcg ggggtaccgg ttttcaacct
     2941 acgcgtcctg gtggattaaa cagggaatga tgcgctcgct ctccaatcat ggaaagacga
     3001 ttcgtattcc ggtttatatg tacgatatta tcgccagatg gcggcgggtt cgtgacgctt
     3061 taacccagag actgaatcgt cctccaacgc ggcgcgagat tgccaaggtg cttgaaattc
     3121 cggtcgcgaa ggtccgtgaa attgaaaaca tcgcgaataa acccagttca ctgaacacgc
     3181 cgatcagtct tgacgggacc gcggagctca ttgatctcat cgaagatgat gtcgccaata
     3241 acccggattc ccgggcggcg gaggtcctga agagtgaaag aattcaaaat ctcctggatc
     3301 atctggacga gcgggagaga aaaatcctca ctttgcgatt cgggtttggg gagcatgacc
     3361 cgcacaccct ggaagaagta gctcagcact tcaatgtgac gcgggagcgg attcgtcaga
     3421 tcgaggcggt ggccttgaaa agaattcgtg cccggcttgt tctcgaaggt gacatcttcg
     3481 aaaactacct tacgcaccag taagtttttt ttgatcacac tttaatcccc gggaggaacc
     3541 catgagtgtt acttcgttaa gatccaaagt acgcgacgtc gcggattttc ccaaaaaagg
     3601 aattcttttc aaggatatca cgcccgtcct cagggacccc gcggcttttc gcgaggtgat
     3661 cgccctgtta gcgaaagccg ttaaaaaatc aaaggcggat ctcgtggcgg gaattgaatc
     3721 ccgcggcttt ctttttgggg ccccggtcgc gacgaaactc gggatcggat ttgttccgat
     3781 ccggaaaaag ggaaaacttc cctggcgcac gaaacgggtt cgttgcacgc ttgaatatgg
     3841 tcaggatgtt ctcgagattc acgcggacgc cgtcaaacgc gggcagaaag tggccattgt
     3901 ggatgatctc ctcgcgaccg gaggaacggc gcaggcggcg gcgaagcttg tccggttaat
     3961 tggcggtaaa gtggttcttc aggccttcgt gatcgagctg gggtttctta acggcaggaa
     4021 aaaactcgcg aagtgcgacg tgtcttcggt gttgaagttc tgaaatccgt tacgcctctc
     4081 gttcaaggga ggaaaatgga aatcgtcttt cgggaaacaa tctgaaaaaa atgcccccag
     4141 catgaatcga acatgcaacc ctctccttag gacggagatg ctctatccaa ttgagctatg
     4201 ggggcaatga ggcagtatta tatgggtatc gatctgaaaa tacaaaattt aaaaaacgcg
     4261 ttgtctttta tttttgtttt gggggcaatt tttatttttg tcacgccagc tttttcggac
     4321 gaagcttcgg acctcaatgt ccagccggag acgatggcgg aatttgagac tctgaaatcg
     4381 gaatgggaaa aagtccgcga ccagcagatc cggatgatcc aggacaagga acaggacttg
     4441 gagcggctga aagaagaact ctttcaaaag atgcgggatg gaaaccccca gaatttctcc
     4501 ggagattatg cgggcacgcc cccgactctt ggggaagatg acaggcttgc ggcggccgcg
     4561 gcggaccccc gggtcatcga atcttttgaa caaaaaatcg cggaattgaa ggaagcgaac
     4621 cggaaacttt cgggtgaaat ttcggaactt aaagcgagaa atcgtgaatt ggaaatggtg
     4681 gcgggcggga gcctcgcttc tgattctgga cgtgatgagc ttcggaagga caaagaggag
     4741 cttgatattc aaaaggcggc gtttcagagg gaaagggaaa aatttttcca ggagatggcc
     4801 cgccaaaggg aaaggcttcg gaaggctgaa ctggaccagg ccggaggcgg tctgaattaa
     4861 gcgatgcctt ctccctcctc taaaaatgcc gtgaggcgag aactcctggc gaggcggcag
     4921 gcgattcccc gctcgaagcg ccttcaaaaa agccggaaaa tcctcaggaa gcttttcaaa
     4981 cacccccgat ttttggcggc aaaaaagatc gcgttctatt ttgatattgc tcctgaagtt
     5041 caaactcgtt tatgtctgga aacgctcgtt aaggataagg aaatctatct tcctcgcgtt
     5101 aacctgaaca cccggcgact ttcgctgtgc cggattcaat cgatcaagaa cgatctccaa
     5161 aggggagcct attcaatcat ggaacccaag gctacgtgtc cccggcgaag tgtctcccgg
     5221 atggaggtga tagttgtccc cggtgtcgga tttgacaaaa aagggaaccg gatggggcgc
     5281 ggcgcgggat attacgatca attattgcgg aaggccggga aaatttttaa gatagggctg
     5341 tgtttccggg aacagatggt tcgccagctt cccgtgacaa aaacagatgt ccccatggat
     5401 tgtgttatta ccgattagac cggaagcggg tctttttttc gccggggcca gacggggaat
     5461 gtgagcgaaa actaaacgaa tgaagatact agacggtaaa atttatacga tcagtgagct
     5521 gaacaaggag gttcgcggcg ttcttgaaac gcgttatacg ggaatttggg tggaaggaga
     5581 gatttcgaac ttcaaacgcc acagttccgg acacatttat ttgacgctga aggatgaaca
     5641 gtcgcaaatt tcggcggttt ttttcagttt ctatcataaa ggactaaagt ttgaaccgga
     5701 ggatggtcac aaggtactgg tgtttgggcg cgtttcggtt tatgaagcgc gggggcaata
     5761 tcaattctac atcgaacgca ttgagcccaa aggagtgggg gcgcttcaac tggccttcca
     5821 gcagttgaaa gaacggttag aaaaggaagg tttgttccgg agcgaacgga aaagaccagt
     5881 gcccaaattc ccaaagacgg ttggggtgat tacttcaccg accggcgcgg cgatccatga
     5941 tatattgaat gtcattaacc ggagattttg cggcacccat attcttcttt accccgtcaa
     6001 ggtccagggg gaaggggccg gcgaagaaat cgcggaagcg attcggatca tgaaccagcg
     6061 cggcgaggcg gaggttttga tcgttggccg cgggggagga agtctcgaag atctttgggc
     6121 cttcaatgaa gagccggtcg ctcgcgcgat ttttgattcc gcgatccctg tcatttcggc
     6181 cgtggggcat gagacggact ggacaatcgc cgattttgtg gcggatcttc gcgctccgac
     6241 tccgtcggcg gccgctgaac ttgtggtggc aagccggggg gaacttgagc tgaagataaa
     6301 agacgcggcg gaacggctaa gacagggaat gatttttcgg ctgagggaac gtcgggaaac
     6361 gctgaaaaat ctggcggcga gcgccgcgtt tcggcaaccg cgctatttac ttgaacagtt
     6421 tatcgagcgg atggatgaat tgggaaagag gctccagact tccctgacga actggatgac
     6481 ttttaaaaaa caggttttcc agaccgcgac cggaaaattg aatgtgttaa atccgttagg
     6541 aatcctggaa cgtggatatt cggtgacgtt tgacgcccgg ggcgctgtgc tcaagtcagc
     6601 cgagaatctt ccgacaggga cagaaatcag aacaagactt catcagggtg aactggtttc
     6661 cagagtgatc gcaacgaagg agacatgttc tccccggact taaaacgccc gacttttttc
     6721 gggttgaaag gatgtccatg gcagagaaaa aagaaaaaat agattttgaa aaggcgatgg
     6781 aaagtctgga aaagacagtc gcggcgcttg aaggcggaga gctcacgctg gatcaagcgc
     6841 tcaaaaaata tgaggaaggg gtcggtcttg tccgtgcctg tcaatcccaa ttgactgaag
     6901 cggagaaaaa gatcgaggtt ctgacccggg cgcttgatgg ttcgttcaaa aaagaacctt
     6961 ttgacgcgtg cggaaagccg gcggcgaagg tcagaaaaaa aggccttgtt cgcggtgaaa
     7021 attcttcaga cgaggaagac taaatggccg attacgggac ctgtttcaaa gtacatctca
     7081 ggctgattga acagacgctc aaaaaagagc ttcaaagtcg cgcgggaaga cccaaggcga
     7141 tccatcaagc catgcagtac gcggtgttta ccggcggaaa acggttccgg ccgattcttg
     7201 ttctcgcggc ttgtgaggct tgcggaggtc ggatgaagga cgcgattcgt ccggcctgtg
     7261 cgcttgagtt gattcatact tactcgctcg tgcacgatga tcttcccgcg ctcgataatg
     7321 atgctgtccg ccggggaagg ttgacgtgtc acaagaaatt cggagaagcg acagccattc
     7381 tggccgggga cgggttgctg accctagctt ttgaaatcct ttcgggagtc caacctgccg
     7441 aaagggcgat ccggcttgtg cgggagatat cgatcgcggc cggagcgcgt ggtatgattg
     7501 caggccaggt cgaagatatt gaaaccgctt ccagacggct tgaccttgcg acgcatgatt
     7561 tcatttgtgc caataaaacc ggaaagttaa tttacgcgag cgtgagagcc ggcgccctgg
     7621 tggccgatgc ttcgccgaaa cagcttgttc tcgttgaccg gtacgcgcgc gcggtcggac
     7681 tggcttttca ggtggtggat gacttgctgg atcaagacgg ttattgccgg tttatgcctg
     7741 aagaccgggt ggttcaaaaa gccggacaat tgataaaaac ggcaaagaag gtagctggca
     7801 aacttggggc caaggcccga tgtttggtgt tattggcgga tttcctggaa tcacgaattc
     7861 cccaaaaata agaggatgtt acaacggtga caaactggat acacaaaatc aatagccctg
     7921 acgacctgaa aaaggtgcct cttgaaaaac tgcccgaggt ggcggaagaa taccgtcagt
     7981 ttctgataga atcggtcgcg aaaacggggg ggcatctggg agcaagtctc ggaactcttg
     8041 agctgacgat ggcgcttcac tatgtgttaa attcaccgac ggacaagatt tgctgggatg
     8101 tcgggcatca ggcgtatgtt cacaaaatga ttaccggtcg acgtgatcaa atggcgactt
     8161 tacgccagcc gggcggcctg agcggatttc cggcgccctg ggagagttgt catgacccgt
     8221 tcatcgtggg acacgccggt accgccattt cgcaggccat cgggcttgcc tgcgcgcggg
     8281 acatcagaaa ggggaacgag caagtggtcg cgatagtcgg ggatggtgcc ctgacggctg
     8341 gcctttcgtt tgaagcgctc aatcatgccg gacagctcaa aaagaacatg ctgattattt
     8401 taaatgacaa cgagatgtcg atcgcgaaga acgtgggcgc ggtcagccgc tatttgaaca
     8461 aagtcctcac tcatccgctt tataacaagg tgcgcggtga aatggaaaag caactccaga
     8521 aatttccccg gcttcaaaaa ttggcgaata cctctcttga tagcatgaag cacctctttg
     8581 tcccgggcgt tctttttgaa gagtttggct tccggtattt tggccccgtg gacgggcaca
     8641 acgtgatcca tttggtcaag attctgaaag atatccttgc cctgaatact tgcagtctcc
     8701 ttcacgtgat aacgaaaaag ggacgaggct gcgaatacgc ggaacaggat caggaaaagg
     8761 ggcatggtgt cgcgccgttc aatgtcaaga ccggagtaaa aatggaaggc gcgagggaaa
     8821 caaattctac gcacgggatc tcgtttaccc aggcgttttc ggacgcaatt ttgaagcatg
     8881 cccgcaggga tccgtcagtg gtggccgtga cggcggcgat gctttcaggg accggtctga
     8941 ctgattttca aaaagttttc ccggagcgct gttttgacgt ggggattgcc gaacaacacg
     9001 cggtcacgtt tgccggagcc atggcccgaa gcgggctcaa acccgtggtg gcggtttatt
     9061 cgacctttat gcaacgctcc catgattcga tcatgcatga tgtggctctc caaagaaccg
     9121 gggtcgtgct tgccctagac cgggcgggga ttgtgggcgc ggatggcgcg acacatcaag
     9181 ggatttttga catggcgtat ctcggttcgg tgccggggat agtgatcggg gctccgaaag
     9241 atgaatttga aatggagaaa atgcttgagc tcgggctgga agcgtccgtt ccctttgtac
     9301 tgcgctatcc gcgggccaat atcgccaaac tgtttgatga atttcccaaa cgttcttttc
     9361 aaacgggtga aggggaggtc atgtttgacg gcgaggatgt gacgcttctc gcgctgggga
     9421 gtatggtgga ggttgcctgc aaggcgcacg cgcttctcag ggaaaaaggg gtctcggcgc
     9481 gagtggtcaa catgcggttt gcaaaacctc tcgatgaaac tctgattctg gagtcgatta
     9541 gaaaaacccg gatgcttttt acgcttgagg agcacgtcat aaccggcgga tttggcgcca
     9601 aagttcttga gttccttgag atcaggaatc tcaggacggt cgccctgaaa cggctggcgc
     9661 ttcccgatga atttatcgaa catggttccc gcgagaaaca actcgactcg tttgggctgt
     9721 ccccggaaaa ggtggctgat tccgtgatca aagaactaag cggtcagaaa aaatgtgaaa
     9781 aatcggcggg ttttgggatc gagcccaacc cgcgctaaca aaggagagat tatcttggaa
     9841 ccgaacgaag agataaaaca gggtgacgcg ggtgttgcgg gcgagacaaa agaagcgaag
     9901 tttaaacgac tggccgaaca gcgagtcgcg gcggcgctcg acaaaatccg gttgatcggg
     9961 aatctcgcga gttcacaata cgcctacaat ggtgagcagg tcgccaagat tttttcggca
    10021 ttaaaaggct caatcgagga catcgaagac aaatttcatc ggacccttga acgaaaaaaa
    10081 gagacatttc acctttaacc tttttttgtg tttcggagct ttctgttttg atctgaaaat
    10141 gagccaaagg tgaaggcttt tggtgtgagt tttgcttaag cgatccagtc caccggagga
    10201 cccaatgata aaaaaaatcg gaattgttgt gaatcagaaa aaaagagggg cgattgaact
    10261 tgccgcgaaa attcgcgact ggctcgagaa gcgggatctt gtggttgtgg attccctgac
    10321 tgagccgatc gaacaattta ttgaagatac ggattttgtg atttctctcg ggggcgacgg
    10381 cacgatgctt tccatcgtga aacatttgcg gaaccgctcc gttccggtat tggggattaa
    10441 tctggggggg cttggttttt tgaccgaagt gaaacaatcg gaagtttttg aagcgttggc
    10501 ggcatatttt cagatccccg agattgaaga cagaatcatg ctgtcttgta ccgcctggag
    10561 caaaaccaag aaaatggagc gccgctttct ggccttaaac gacatcgtga tcagccggga
    10621 agggctcgcg cggattttgc gcctggatgt ttttgtgtcc ggcgaaaaac tcaccagttt
    10681 ccggggagac ggggtgatta tcgcgacgcc gacgggttcg accggatact cgctttccgc
    10741 ggacggggcg gtggtgcatc ctaaactcga agtgtttatc attaccccga tctgtccgca
    10801 cgcgctcagc ctccggtcgc tcgtgattgg cgccgacgaa aaaataatgg tgcaggttcg
    10861 cacggatgat cccgctgaga aggctctgct gacggctgat ggtcaagaaa aagtggaaat
    10921 cgacgatagc tatatcgttg aaatcgggcg gtcgaacgcc ccgtttaaat tgatcaaaag
    10981 cacgaagcgg agttaccttg aaactctcag agaaaaattc aaacttcccg ataccgaagc
    11041 gtgaggagca aggtcggttg ctcattgtgg atgacgagac gtcgatttgc gaagagtttt
    11101 cggaaaccct ggagcacgaa g
//
LOCUS       DAOSIO010000033        10928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_5915_length_10928_cov_4.346455, whole genome shotgun sequence.
ACCESSION   DAOSIO010000033 DAOSIO010000000
VERSION     DAOSIO010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10928
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5915_length_10928_cov_4.346455"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..950
                     /locus_tag="PK590_02260"
     CDS             183..950
                     /locus_tag="PK590_02260"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HOG23453.1"
                     /translation="MDVHETIHKVAELLKKSKSILFITGAGVSADSGLPTYRGLGGLY
                     DDVETEDGIPIEMALAGDTLRKRPEVTWKYLSQVEQKCRGARPNRAHEIIAEMERRFE
                     RVWVLTQNIDSFHHVAGSRNVIDIHGDMHDIQCEECGWKTRVENYDGLEIPPRCPKCD
                     AIVRPDVVFFGECLQPGKVRSMEREIGRPFDVYFSIGTTSVFPYIKHPMILASHLGRP
                     TIEINPDTTDISGWVDIRIPMGAAVALDALWQELAAP"
     gene            complement(1299..3566)
                     /locus_tag="PK590_02265"
     CDS             complement(1299..3566)
                     /locus_tag="PK590_02265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG23454.1"
                     /translation="MTPARGKEDPVIFFTKIRFILTLGLLAILATGAFMLLIYEVKRA
                     EHDALVIATMENQWGFIHKANFLASEYAQSNTPPERQILRKEVHEALSLTLVFTSNQP
                     ASLALSSLPADLQRTLYHLYCHPPSRLNDEILDYINSVKNFMMGTPVHVSSDYPQLRA
                     LNAQTLRVLSMLRGSVRQYQRESLRRIVFLKNLGIVFFASTLLALLITGVLTFIPILR
                     KIRGYLDQLKNVNQTLEKKVRERTLELEQKAVELSASNQQLQDEVQERLRAENNLRNA
                     NVFLDSVIENIPDMIFIKDANELRFVRFNRAGEQLLGYSRKELIGKNDYCFFPREQAD
                     FFIQKDRDTLKNKVLLEITEEPINTLKKGPRILHTKKIPILDEAGNPIYLLGISEDIT
                     QRIMTEKQLEENMTAMENALDGIARLDKNMNFLMVNKAYAAMLGYTPEEMTGLNLFST
                     ISQEDQNKVKTASETLEKTGKLELEVKALRKDKSVLHQFLVLIPPGGENKFFDGFHCF
                     ARDITEHKYRESVEIKSDLIQMVSHELRTPIHSVKEGINIVLEGLTGPLNAEQRETLE
                     ISKRCADRLTRLVNDVLAFHKLDAGVIEFRIKKTDINALVCDVAESMGPLVKEKNLRF
                     EVKTDASVPAADMDPDKIVQVITNFLQNAIKFTSQGSITLSTAFKNNRVVVSVQDTGI
                     GIQQKDLPKIFKKFSQLESAQSIAPGGTGLGLAISKKIVETHHGTVSVESEYKKGSLF
                     SFSLPLSQPVTNPGA"
     gene            complement(3563..5257)
                     /locus_tag="PK590_02270"
     CDS             complement(3563..5257)
                     /locus_tag="PK590_02270"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG23455.1"
                     /translation="MIEKRGDVTSWNILLVEDDLDFANILKIRLSKERNPSLAITISP
                     SLERALDSLRINHYDLILLDLMLPDSSGIETFSKIKAEARHKPVVIITGLDNDQVAID
                     AVRRGAEDYLVKSDISSRFLTRIIHHAIDRHRIKEKLASVTGRLRETSLRLEKMALMD
                     PLTELFNRRGLQQALTRELQLMLREGHGLLALLLDIDNFKHINDSLGHPVGDMLLKEI
                     AKKIKEVVRESDHVARIGGDEFMLLLPKTSHGEGIHLAERIRMTIADTVIYVSDRDTL
                     QITASIGLTPVSSNVTSVDELLAIANPLLRQSKQKGKNCISLDEEEVSCPDAKISTYA
                     EQLSAVLRRGTDFFALKQPILNLTDLSVIGYEFLSRLNNGSIVMPNDFFRVAREQNML
                     TLVDHYCFRSCVSASAPLAPELYRHINLFPATIVDISPDEVVEKLSVSCPAKSYCLEI
                     SEQQILGDPSHLIDPVETFKRSGVSIAIDDVGFGNTCLENLILLEPDVIKLDKRYIRG
                     IARDAQIEKTLRRVLKIAEDLDAEVIAEGIESREDLDTLRGLGVKYGQGFYLGAPA"
     gene            complement(5271..5717)
                     /locus_tag="PK590_02275"
     CDS             complement(5271..5717)
                     /locus_tag="PK590_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG23456.1"
                     /translation="MKVQNDTPVASQSGSALELQMPNTQKKRILVVEDEPSANKFLTL
                     RLQKLGYEVIAVFDGATGLREAIDKIPDLLILDLMIPKLPGEEVCKALRESFDERIAK
                     IPIIMLTGKDTLVDKVVGKVIGADAYLTKPYEFNDLLTEINRIMSS"
     gene            6172..8037
                     /locus_tag="PK590_02280"
     CDS             6172..8037
                     /locus_tag="PK590_02280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOG23457.1"
                     /translation="MDLVMGAKYLAWLCPVGCVLAAAKIGRLCFPGAFLRDRYLLVLF
                     HAGAGLVIFSMVLFAAVLMGLATRPVLWRITSFCLILGLHELIRLISGIRGKRSLLRV
                     HLIPVTLLSVYFFLQGILTLLPPTACDETIYHLHVPQKWLETGGKYFFADNIYAYFPQ
                     LADLYFLLGLGALGEPAAKFFHVTWGALLAGAIFRVSRKWLGPRQAWCPVFVWLLIPS
                     VLVTMHWAYVDLTFAFYAFMALVLLMEYRDIPSRRTWAGCALMAGGAAATKYTGIQFA
                     LILACLFMILHRRRQARQIWEPILVLGGLAGLVLSPYLVRNFIQTGYPLFPFAFPGLT
                     VHSVNWDPDRAMLFVSWLQTFGTRIGEESAWDTLWAPIRIFIKGRFFDHVNFDGIIGP
                     TFLLIPFFFREIKKDSGLRVLGLFSLFFVVYWTLTTKQVRFFFPVLPALSILLVCGAA
                     RAGGKRLLGVVMVLAAGLSWLGFREIVRVDPVPYLSGEEGREEFLRRRIDVYPIYKES
                     MKQLGPDDKVYLLDMRNHGYFLDKPWTGDYVFEHYNLERFLKGLKSPKEVGRFFSDRG
                     VTHLLVGTDITFSETMGLEKREALLFSSFLRENALPLLARRKHIFFKLKETGEAT"
     gene            8034..8717
                     /locus_tag="PK590_02285"
     CDS             8034..8717
                     /locus_tag="PK590_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG23458.1"
                     /translation="MKLSVVMPVYNEKKTVEEVIRRVLGLAMEKELVIVDDASTDGTR
                     EILARYENTPHVRIFYHEKNQGKGAALQTGFRGVTGDLVVIQDADLEYDPKEYPDLLE
                     PILDGRADVVYGSRFLGGPHRVLYYWHYVGNCFLTMLSNMLSNINLTDMETCYKAFRR
                     EVLDKVEIRSRRFGCEPEFTMKVAKHKFRIYEVPISYSGRSYEEGKKITWKDGLAAIF
                     TMIYYRFFD"
     gene            8893..9129
                     /locus_tag="PK590_02290"
     CDS             8893..9129
                     /locus_tag="PK590_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23459.1"
                     /translation="MGSEKRSVTLDIIVSEVFKWIGFAWTGLGVFFTLQFFEAIAEGD
                     KITNLEYNMRMYQLALEWAIVAIPAFVASYFGKK"
     gene            complement(9975..10499)
                     /locus_tag="PK590_02295"
     CDS             complement(9975..10499)
                     /locus_tag="PK590_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HOG23460.1"
                     /translation="MMQTERLRGVVVPKTLRIVPAGSKGFSLVANRKFRLGEKVCFLA
                     GELVDAAHSTPEAVQISDTQFIDTNYLVAEDFINHSCSPNTRLDIECRQFTALRDISR
                     GEEITYHYLTTEWDMEAWGADFRCACGSLHCLGHIRGFKYLPLAEKLRIKPLLSPLLR
                     GKLEEIERLPQEKI"
     gene            complement(10519..>10928)
                     /locus_tag="PK590_02300"
     CDS             complement(10519..>10928)
                     /locus_tag="PK590_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homospermidine synthase"
                     /protein_id="HOG23461.1"
                     /translation="PSLRIITDEDIKGNDALGALIMGHPYTSWWTGSILDIREARKLV
                     PHQNATTIQVAIGVVAAVTWIIKNPTRGVCQPDDLPHDEVLKVAKPYLGTFVSEPSDW
                     TPFKNYQSFFPKNPQAKLDRKNPWAFQNFLHRD"
BASE COUNT         2528 a         2442 c         2979 g         2979 t
ORIGIN      
        1 tggggcagcc tcctacaggc ctcaagaaat taactgccta aagtgggata caccctcaag
       61 atgcttgagt tagatcgcga aatacattag gctcactatt ttgagaagaa tcggcgctgg
      121 gacgcggcca tggattctgc cgcgccgata ttgcaggcaa acttttaccc cggagcattt
      181 gaatggatgt ccacgaaacg atccataaag tcgcggaact gttaaagaag tcaaaaagca
      241 ttctgtttat caccggagcg ggggtttccg cggattcggg gttgccgaca tatcggggac
      301 ttggcggact ttatgatgat gttgaaaccg aagacggtat tccgatcgaa atggcgctgg
      361 caggggatac gctccggaag agacccgagg taacctggaa atatctttcc caggtcgaac
      421 agaaatgtcg cggagcaaga ccgaaccggg cccacgagat cattgccgaa atggaaaggc
      481 ggttcgagcg tgtttgggtt ctcacacaaa atatcgacag cttccatcac gtggccggct
      541 ccagaaatgt gatcgacatc catggggaca tgcatgatat ccagtgtgaa gaatgcggtt
      601 ggaagacccg cgtcgagaat tatgacggtc tcgagatacc tccgcgctgt cccaaatgtg
      661 atgcaatcgt ccggccggat gttgtctttt ttggcgagtg cttgcaacct ggcaaagtca
      721 ggtcgatgga acgggagatt gggcggccgt ttgatgtcta tttttccatc ggaacgacaa
      781 gtgtttttcc ctatatcaaa caccctatga tcctggcctc acatcttggg cgtcccacga
      841 tcgagattaa tcctgacacg acggacatct ccgggtgggt ggatatcagg atccccatgg
      901 gcgccgccgt agctcttgat gccttatggc aggaactcgc cgctccctaa ttgatgctgg
      961 cgcagatgag cgtacaaccc agacggagga aagcatcatg gacgtccgaa tggcgctcgt
     1021 aatagattca cagacgacga aatcagttca gccagtttaa ggaccgttca attaaccatt
     1081 gatcattgcc agggtgatga ttgctacgag acgtcgatca atttttggct ggatattcct
     1141 tttttgaagt ttgttacgga aatccgcgga actgtagcct tttcgattcg gaacgggtgg
     1201 ccgcccattg gaacccctgg gtttagttag gcgctttact ttaataaaac gcgacatgag
     1261 tttgtcagag cggattggaa atacctgtgt aaccgcctct aggcgcccgg attggtgacg
     1321 ggctgggaga gcggcagaga gaaggaaaac agactgcctt ttttatactc cgattctacg
     1381 gaaacggttc cgtgatgcgt ctccacaatt ttttttgaga tagccaatcc aagtccagtt
     1441 ccgcccggag cgatgctttg cgccgattcc agctgggaaa attttttgaa aattttgggc
     1501 agatcttttt gctgaattcc gatgcccgtg tcttggaccg aaactaccac gcggttgttt
     1561 ttaaaggccg tcgagagggt gatcgaacct tgagaggtga acttgatcgc gttttgcaga
     1621 aagtttgtga tgacctgcac gatcttgtca gggtccatat cggctgccgg aaccgacgcg
     1681 tctgttttaa cttcaaaacg aaggtttttt tctttaacga gcggccccat ggattccgcg
     1741 acatcacaaa caagggcatt gatatctgtt tttttgatcc ggaattcaat gaccccggcg
     1801 tccagtttgt gaaaagcgag gacgtcgttc acgagacgcg ttaaacggtc cgcgcatctt
     1861 tttgatattt caagggtctc tctttgctcg gcatttagcg gccctgtcag cccctccagg
     1921 acaatgttga tgccttcctt gacggagtga ataggagttc gaagttcatg ggaaaccatc
     1981 tggatcaggt cggacttgat ttcgacggat tcccggtatt tatgttccgt aatatctctg
     2041 gcgaaacaat ggaatccgtc aaagaatttg ttctcgccgc cgggggggat caggacaagg
     2101 aactgatgca acaccgattt gtcttttcgg agggccttga cttccagctc caatttcccg
     2161 gtcttttcca gtgtctcgga agcggttttt actttattct ggtcttcctg gcttattgtt
     2221 gaaaacagat tcagtccggt catttcttcc ggcgtgtatc ccagcatggc ggcataagcc
     2281 ttattcacca tcaaaaagtt catgtttttg tcgaggcggg cgattccatc cagggcgttt
     2341 tccatggcgg tcatattttc ctcgagttgt ttttccgtca tgatccgttg ggtgatgtct
     2401 tccgaaatcc cgagcagata gattggattt ccggcctcgt cgagaattgg aatttttttg
     2461 gtgtgtaaaa tccgaggccc ttttttcagg gtgttgatgg gttcttcggt gatttctagt
     2521 agcaccttgt ttttgagagt gtcgcgatct ttttgaatga aaaaatcagc ttgttccctg
     2581 gggaaaaagc aatagtcgtt tttgccgatg agctctttgc gggagtaacc gaggagctgt
     2641 tcgccggccc ggttgaagcg aacaaaacgc aattcattcg cgtccttgat aaaaatcatg
     2701 tccggaatat tttcgatcac ggaatccaga aagacattcg cgttccggag attgttttct
     2761 gcccgcaatc tttcctggac ttcatcttgg agctgttggt ttgacgctga aagctcaacg
     2821 gctttttgtt ccagttccag cgtccgttcc ctgacttttt tttcaagggt ctgattgacg
     2881 tttttcagct ggtcgagata gcctctgatt ttccggagga tggggatgaa ggtgagaact
     2941 ccggttatca gaagagctag cagggttgag gcaaagaaaa cgatccccag atttttcagg
     3001 aagacgatgc gccggaggct ttccctctgg tattggcgaa cgcttccccg aagcatgctt
     3061 agcacgcgca gcgtttgtgc gttcagtgcc cgaagctggg gataatcaga actgacatgg
     3121 accggagtac ccatcatgaa gttttttacg gaattgatat agtccagaat ttcgtcgttg
     3181 agccgggagg gaggatggca atagaggtgg tagagagtcc gttgaagatc cgcggggagc
     3241 gaagaaagag cgaggctcgc gggttggttg cttgtaaaaa caagggtcag agaaagggcc
     3301 tcatgaactt cttttctcag gatttgccgc tctggcgggg tgttggactg cgcgtattca
     3361 gacgcgagaa agttggcttt atgaataaat ccccactgat tttccatggt cgcgatcacc
     3421 agcgcgtcgt gctcggcccg ctttacctcg taaatcagaa gcatgaaggc gccggtagcc
     3481 aggatagcca acaggcccaa cgtcaggata aaccggattt tcgtgaagaa aatgacgggg
     3541 tcttccttgc cgcgagctgg ggtcatgcag gggctcccag ataaaaaccc tgcccgtatt
     3601 tcacgccaag ccccctgagg gtatcaagat cttcccgcga ctcaatccct tccgcgatca
     3661 cttcagcgtc caggtcttcg gcgatcttga gaacccgccg gagtgttttt tcgatctgag
     3721 cgtcccgcgc gattccccgg atatatcttt tgtccagctt gataacgtct ggttcaagga
     3781 gaataaggtt ttcaaggcaa gtgttcccaa atccgacgtc gtcgatcgcg atggaaacac
     3841 cggaccgttt aaaagtttct acggggtcaa tcaggtggga aggatccccg aggatttgtt
     3901 gttcgctgat ttcaagacag taacttttgg cgggacagct gacggagagt ttttcaacga
     3961 cttcgtcggg agaaatatca acgattgtcg cggggaaaag gttgatgtgc cggtaaagct
     4021 cgggggcgag gggagcggag gccgaaacac aggagcggaa acaataatga tcgacgagcg
     4081 tgagcatgtt ttgttctcgc gcgacccgga aaaaatcatt cggcatcacg atggagccgt
     4141 tgttcagacg gctcaaaaat tcataaccta taacggaaag atccgtcaga ttcaggatgg
     4201 gttgtttgag cgcgaaaaaa tctgttcccc ggcggaggac ggcggagagc tgctcagcgt
     4261 aggtggagat tttggcgtcg gggcagctaa cttcctcttc atccagggag atgcaatttt
     4321 ttcctttttg tttgctctga cggagcaaag gattcgcgat cgcgagcagt tcatcaacgg
     4381 atgtcacgtt gcttgaaacg ggggttagcc cgatacttgc cgtgatttgg agcgtatcgc
     4441 gatcactgac ataaatcacg gtgtcggcga tagtcattcg gattcgctcc gccagatgga
     4501 tcccttcgcc atggcttgtt ttggggagaa gaagcatgaa ttcatctcct ccaattcgcg
     4561 ccacatgatc gctttcccga accacttcct tgatcttttt cgcgatttcc ttaagaagca
     4621 tatcgcccac gggatgtccc aaagagtcat tgatgtgctt aaaattatcg atatcaagga
     4681 gaagcgcgag gagaccgtgg ccctctctca gcattagctg aagctcccgg gtcaaagcct
     4741 gctgaaggcc gcgccgatta aaaagttcgg tcaagggatc catcaaagcc attttttcga
     4801 ggcgtaaact ggtttccctg agccgtccag tgacggatgc cagcttctcc ttgatccggt
     4861 gacggtcaat cgcgtggtgg atgattcgcg tgagaaagcg cgagctaatg tcgcttttga
     4921 ccaggtaatc ttccgcgccg cgtcggacgg catcaatcgc gacttgatcg ttatcgaggc
     4981 cggttatgat gacgacgggt ttgtgcctgg cttcagcctt gatctttgag aaggtttcaa
     5041 tgccggagga gtcaggaagc atgagatcaa gcaggatgag atcatagtgg ttgatccgga
     5101 gagagtcaag ggccctttca agggaaggag agattgtgat agcgagcgac gggttccttt
     5161 ctttactcag gcgaatcttc agaatgttag caaagtctaa atcatcttca accagcaaga
     5221 tattccaact cgttacgtcg ccgcgctttt cgatcatgcg ttctcccttc tcaggacgac
     5281 atgatgcgat tgatttctgt taaaagatca ttgaactcgt agggctttgt cagataagcg
     5341 tctgccccga tgacttttcc gactaccttg tcgaccaggg tatccttgcc ggttaacatg
     5401 atgatgggaa tcttggcgat cctttcgtca aagctttccc gaagggcttt gcagacttct
     5461 tcgccgggaa gtttggggat catcagatca agaattaaaa gatccggaat tttatcgatg
     5521 gcttccctga ggcctgtcgc gccgtcaaac acggcgatga cctcatagcc aagcttttgg
     5581 agccttagtg tgaggaactt gttggcgctt ggctcatctt cgacgaccag aatacgtttt
     5641 ttctgtgtat ttggcatttg caactcaagg gcgctcccgg actgcgaggc gacaggggtg
     5701 tcattttgca ctttcatgac catgcgagcc tcctcatgaa aggagctact ttcttatgcg
     5761 ttttcaggat acaatccgct atagaatgac actttcttaa attctaaaag aagtatactc
     5821 cctcacccgt caaaaagcaa atcaggccgt tttttggcct cggcacggag ttttgccatt
     5881 gggcttgtgg ggctcttggg tcaaattttt cacaaagaaa cagcgggggc gctttcacaa
     5941 atattttcaa cccacttttc ctccagggac tggaaagccc aaaaagagcc tatgaaaccg
     6001 ttttcaattt tttgacgggg tgcgtgtcaa aaggttgatg gaatctcaaa tggatgaatt
     6061 gcgctctaca aacttgaaat cctgatttac aattcccaag cagaaagatt tctcgctcgg
     6121 agatgataat ctgcgggcgg tcaaagcttg taacaaaata cggggaaaca aatggatcta
     6181 gtgatgggag cgaaatattt ggcgtggtta tgtccggtgg gctgtgtttt ggccgcggca
     6241 aaaataggcc gcttatgttt tcccggagct tttttgcgag atcgatatct tttagtgttg
     6301 tttcacgcgg gagcgggtct tgtcattttt tccatggtgc ttttcgcagc cgttttaatg
     6361 gggctggcaa cgcgcccggt tctttggcga ataacaagtt tttgtcttat cctgggtttg
     6421 catgaactga tccggctgat ttctggaatt agggggaaaa ggtcgctttt gcgtgttcac
     6481 ctgataccgg ttacattatt gtccgtgtat ttttttctcc agggaattct cactcttttg
     6541 cctccgacgg catgcgacga gacaatctac catctccatg tccctcaaaa gtggctcgaa
     6601 acgggcggaa aatatttttt cgcggataat atttacgcct atttcccaca gctcgcggat
     6661 ctttactttc tgctgggact tggagcgctg ggggagccgg ccgcgaaatt ttttcatgtt
     6721 acttggggcg ccctgctcgc gggtgcgatt tttcgagtgt cgcggaagtg gcttggcccg
     6781 cggcaggcct ggtgtcctgt ttttgtgtgg cttttgatac catctgttct ggtcacgatg
     6841 cactgggctt atgtcgactt gacctttgcc ttttacgcgt tcatggcgct tgttctcctc
     6901 atggagtacc gggacatccc cagtcgaagg acgtgggccg gatgtgcctt gatggccgga
     6961 ggagctgcgg cgacaaaata tacgggaatc cagttcgcct tgattctcgc ctgccttttt
     7021 atgattttgc acagacgacg tcaggccaga cagatttggg agccgatact tgttttgggg
     7081 ggactggcgg ggctcgtgct ttccccgtac cttgtccgaa actttattca aacaggttac
     7141 cctcttttcc cgtttgcttt tccgggacta accgttcatt ccgtaaactg ggatccggat
     7201 cgggcgatgc tttttgttag ctggcttcag acatttggca cgcgcattgg agaggaaagc
     7261 gcgtgggaca ctctttgggc gccgatcagg atttttatca aaggccgatt ttttgatcat
     7321 gtcaatttcg acgggatcat cgggccgact tttttgctga taccgttttt ttttcgggaa
     7381 ataaaaaaag attcagggct tcgggttctc gggctctttt ctctgttttt tgttgtctac
     7441 tggactttga cgacaaaaca ggtccgtttt ttctttcccg ttttgcctgc gttgtccatc
     7501 ctgcttgtct gcggcgcggc gagggcaggg gggaagcggc ttttgggagt tgtgatggtg
     7561 ctggcggctg gtttgtcctg gttggggttt cgggagattg tgagggtcga tcccgttccg
     7621 tatctgtcag gcgaggaagg acgagaagag tttctccgac ggcgaatcga cgtatacccg
     7681 atttacaaag aatccatgaa acaattgggc ccggatgata aagtctatct tctggatatg
     7741 agaaatcacg gctattttct tgataagccc tggacggggg attatgtgtt tgagcattat
     7801 aatctggaac gatttctgaa aggtctgaag tcgccgaaag aagtcggccg ttttttttct
     7861 gatcggggag tgacccatct gctggttggt acggacatta cgttttcgga gaccatgggg
     7921 cttgaaaagc gggaggcctt gcttttcagc tcttttttaa gagagaatgc cctgcctctt
     7981 ctggcgcgga ggaaacacat attttttaaa ttgaaagaaa cgggagaagc gacatgaaac
     8041 tatccgtggt gatgccggtt tacaatgaga aaaagactgt ggaagaagtg ataaggcgag
     8101 ttttgggact tgccatggaa aaggagctgg tgatcgtgga tgacgcgtcg acggacggca
     8161 cccgggaaat tctggcgcgt tatgagaata ccccgcatgt ccggattttt tatcatgaaa
     8221 aaaaccaggg caagggagcc gcgcttcaga cagggtttcg cggcgtgacg ggggatctgg
     8281 ttgtgataca ggacgcggac ctcgaatatg acccaaagga atatccggat cttcttgaac
     8341 caattctgga cggccgtgcc gacgttgttt atggatcacg ttttttgggc gggcctcacc
     8401 gtgttcttta ctactggcac tatgtgggga attgtttttt gactatgctt tcgaatatgc
     8461 tttccaatat caacctaacc gatatggaaa cctgctacaa ggcgtttcgt cgcgaggtcc
     8521 tcgacaaagt tgaaattcgt tcccggcgtt ttggctgtga gccggaattt acgatgaagg
     8581 tagcgaaaca caagttccgg atctatgaag tcccgatcag ctacagcggg cgaagctacg
     8641 aagaaggtaa aaagatcacc tggaaagacg gattggccgc catttttacc atgatctatt
     8701 accgattttt tgactgattc ttccgctccc agacgtggct atccgcgccg gtgataacaa
     8761 tcctgtggga tgagttggtc acgctttagg gcgggcttgt ttgggtcgat aagggacgca
     8821 gaagtcatct aaaacttaag ttttccttag atcgcaagag aaatcgggag aaaacaaaag
     8881 ggggttgcgg tgatgggaag tgaaaaacgt tcggttacat tggatatcat cgtatctgaa
     8941 gtcttcaaat ggattggatt tgcatggact ggattgggtg ttttttttac gcttcaattc
     9001 tttgaggcta ttgcggaagg cgataaaatc acaaaccttg aatacaacat gagaatgtat
     9061 caactcgcac ttgaatgggc gatagttgcg atacctgctt ttgtcgcatc ttactttggc
     9121 aagaaatgag ttatcgacga acggaaaagg ggtttgggaa tgaacggtga aagaataatt
     9181 atgggagagg gagggggagg ttatgaggat ttttggcgca ttgtttttgt gtttgctttt
     9241 agttggatgt gcgacaaggg caaaaatgaa caatctcagt cttggtatga caaaatccca
     9301 ggttctaagt gttatgggac ggccggcttc gacagcggct acgcatggca cagaagtatt
     9361 aaggtatgat ttgcatgata acgtatttga cgaacacgca agcgaatatt gggttacatt
     9421 ggaaaatggg agagtgtcag aatacggtcg ggcaggagat tggaaatagt gcaatctatt
     9481 atctggacaa tcaaaatcct aataacaaaa tgcccgaatg aataagaaat acaacgatct
     9541 tgacgctaag ctctccaggt tgaccgccgt ttttttcagc cattatctcg acatgaacta
     9601 ctaaaatcca gggacggttc ttgctagtcg tggcgccctg ggagtccgga ggcgcgtgat
     9661 ttctgacatc cctggggttc atttgtgagt ttgtttgctg agggtgcggg cgaaatcttt
     9721 tcctctgaat gttctgacag gtacagatct ggtttcgaat tacttaaaat gatacttgcg
     9781 gatttgccca agggagcagg tcgagggagg gcttacctct tcaggtgccg gaagttgccg
     9841 tgctatagtt tctcaaacag gaaattccgc tttccctaaa ttgggcatat ttcgatttat
     9901 tcgactattg gaatacggtt gatttgggaa ccggtcctga tgcctggtaa ataaaccgca
     9961 taaacgcgcg attttcaaat tttctcttgt gggagtcgtt ctatttcctc gagttttccc
    10021 cggagcagag gagacaaaag cggttttatc cggagttttt cagcgagggg cagatattta
    10081 aaaccccgaa tatgcccgag gcaatgaagg gaaccgcagg cgcaccggaa atcagctccc
    10141 caggcttcca tgtcccattc ggtagtcagg tagtgatacg taatctcttc gccacgggaa
    10201 atatcccgga gagcggtaaa ctgccggcat tcgatgtcaa gccgtgtgtt gggtgagcaa
    10261 ctatggttta tgaaatcctc agctaccaga tagttcgtat cgatgaactg ggtgtcggaa
    10321 atttggaccg cttccggggt tgaatgggcg gcgtcgacga gctccccggc cagaaagcag
    10381 accttttctc cgagacgaaa ttttcgattc gcgacaagcg aaaagccctt actgcctgct
    10441 ggaacaatcc tcaaggtctt cggaacgacg actccgcgga ggcgttcagt ctgcatcatg
    10501 gatcctcttc ttgtgtcgtt agtctctgtg cagaaagttt tgaaaagccc aggggttttt
    10561 cctgtccagc tttgcctggg gatttttcgg aaaaaaggac tggtagttct tgaacggggt
    10621 ccagtcgctc ggttccgaaa caaaagttcc cagataaggt ttcgcgacct taagcacttc
    10681 atcgtgggga agatcatccg gctggcagac tccccgcgtc ggatttttga ttatccatgt
    10741 gacggccgcc acaactccga tggcaacttg gatggtggtg gcgttttggt gcggaacgag
    10801 ttttctggct tcccgaatgt ccagaatact tcccgtccac caggaagtgt acgggtgtcc
    10861 catgatcagg gcccccaagg cgtcattgcc tttaatgtcc tcatctgtga tgatccgcag
    10921 agaaggct
//
LOCUS       DAOSIO010000034        10866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_5948_length_10866_cov_4.545833, whole genome shotgun sequence.
ACCESSION   DAOSIO010000034 DAOSIO010000000
VERSION     DAOSIO010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 10866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10866
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5948_length_10866_cov_4.545833"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..642
                     /gene="tmk"
                     /locus_tag="PK590_02305"
     CDS             22..642
                     /gene="tmk"
                     /locus_tag="PK590_02305"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOG23462.1"
                     /translation="MKKKGIFITFEGAEGSGKSTQIREAAKFFRKKGREVILLREPGG
                     TKISELIRKILLDRANQGMSHATELFLYLAARAQVVEEKILPALREGKVVIADRFEDS
                     TRAYQGFGRGFSLEAIESMSRLVRGTLIPDLTFVLDVDITKGLARGGRHDRIEWEALS
                     FHRKVRQGYLTLARKEPGRMLVLDTDQPVSVVKQCVLERLQRVFGK"
     gene            632..1504
                     /locus_tag="PK590_02310"
     CDS             632..1504
                     /locus_tag="PK590_02310"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23463.1"
                     /translation="MANKTQKLAARILESHMARRRVSHAYIFSGSGEALEMAELAGER
                     GSADIKEEFAVAFAAALLSGSEKLFQVAESSISARIWKRNHPDVRWIGEDQEERSIKV
                     GEDSSERGTIREIIHWAGIKPYESPWKLCIVLKSERLTDVAANAFLKTLEEPPPHTVF
                     LLLVENKTHLLETIQSRCFEIRIPASESFAVPRSLAGLATIPVKEILESYPSLSKEDL
                     RRKLDGLMPFIREKITNLVSADSPDLDRWLEAMDLVYDSKAALGDNVNYKLMLTRLAM
                     RLSRILPVDGKGLI"
     gene            1504..3048
                     /gene="metG"
                     /locus_tag="PK590_02315"
     CDS             1504..3048
                     /gene="metG"
                     /locus_tag="PK590_02315"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOG23464.1"
                     /translation="MTNYYLTTPIYYVNGKPHIGHAYTSILCDTFARFHRMLGEEVFY
                     LTGTDEHGIKVQKAALSEGKTPQAFVDEIMPKFRELWTLLGVRYDYFIRTTDAEHKAG
                     VQKTLSALEAQGDIYKASYHGWYCTPCESFWTELQLKEGKCPDCGRAVERLEEENYFF
                     KLSKYQGWLMDYIQTHPDFIRPDYRRNEILGFLREPLEDLCITRPKSRLSWGIEYPGS
                     QDHVVYVWFDALLNYLTALGYPAEESKFKKYWPVDCHMLGKDILRQHAVYWPIMLRAM
                     GVEMPKLVLAHGWWKIGGEKMSKSLGNIVDPVAIVKKYGSDAFRYFLMREVTLGLDGT
                     YSEDLLKERYTSDLANDIGNLWHRTASMMEKYFGGKVPTSSQGAEKTPLWQNVIGLWD
                     KVCRDMLAYDPRESLAKIWTVVTQANQFVEEKKPWVLAKHEDQKQELADTLYVLAETV
                     AHIGVLLQAFLPETSRKITSRLKLSDPFAIKDESAFRKPLVKAGTVIEKGDPLFPRLE
                     DKIAPC"
     gene            3042..3809
                     /locus_tag="PK590_02320"
     CDS             3042..3809
                     /locus_tag="PK590_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOG23465.1"
                     /translation="MLTDTHLHLHFSQYDLDRDEVIRRAIAGGVGLMINVGTDLEDSR
                     KAVQVAETYPEVYAAVGYHPHESKNASREALAELEKLIAHPRVVGVGEVGLDYFHNHS
                     PQETQREIFRVFLSWYRRHQKPIILHCREAYEDMLRLLKDELELPCRGVIHCYSSDSE
                     MMFKFLELGFYVAFGGALTYKKNETLREACRQCPLDRLLIETDAPYLAPQSRRGERNE
                     PLFLVETAKFIASLRGCDPEKLFTETTANARRLFDLC"
     gene            3848..4897
                     /locus_tag="PK590_02325"
     CDS             3848..4897
                     /locus_tag="PK590_02325"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOG23466.1"
                     /translation="MLLIVLIGVFYAAITTGTGRKIRASQAVPSQAMEEFKAAERQFN
                     EQSFSGWASEEFRNQNPLLTRSFEFFTLFFLLAIVAGLGIDFILLVRPSLRKKLLYRP
                     DPPPVREWPFSILLRTVVLFITWGIVLSVAIGLLESFFLKTGSENLLMILHTLILDVL
                     GLILVVYFLKKQGSSWRELGFNIPQGKVFREMGIGITGYLGTLPLFVLVLMALMYFSS
                     LFSYEPPPHPLVNVFVEEEKRQPFLVIFSILFGAVIGPVFEEIFFRGFCYPILKGKIG
                     KVGAMVVSSAFFASIHHTGFVFWPIFILGMALAYLYETRRSLLAPMTLHVVHNSLLIG
                     YFFLAKQVISSSHPL"
     gene            4912..5976
                     /gene="mtnA"
                     /locus_tag="PK590_02330"
     CDS             4912..5976
                     /gene="mtnA"
                     /locus_tag="PK590_02330"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006773644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOG23467.1"
                     /translation="MQNKKVPFAPIKFSRHVLYLLDQRKLPIREVWVACRNSRMVWKA
                     IRDMIVRGAPAIGIAAGYGLYLGVRHFQGDQKGFWETLRDQGRFLDSARPTARNLACA
                     LERIMKAVHCAGDRSVEELKKLVLAEAIRIHHEDIDLCRKIGVFGSELFKKGDVLLTH
                     CNAGGLATSGYGTALAVFYALKEKKIPFSVFVDETRPLMQGARLTAWELQKSKIPATL
                     ICDNMAASLMRAGKIHGVVVGADRIAMNGDTANKIGTYGVAVLAKVHGIPFYIAAPST
                     TFDLKAKTGEDIPIEERPHHEVREIQGRPLAPKGMKVFNPSFDVTPHGYISGIITEIG
                     VLRPPFTKSIRRIGTLLEKS"
     gene            5963..6970
                     /locus_tag="PK590_02335"
     CDS             5963..6970
                     /locus_tag="PK590_02335"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-monophosphate kinase"
                     /protein_id="HOG23468.1"
                     /translation="MKNHSAGVVAGLTEGMGMNEIDFIEHLRRKISRSRAVSVGIGDD
                     AAVLAPLRRGRLVISTDMLVENVDFRIKKDGQRKAPDDFLTPEQIGRKALAVNLSDMA
                     AMGAKPLAFVLTIGKPTYISTKWLDRFYNGMMRFAREYGVNCIGGDFSRAREFSVSVT
                     ILGVAEKPILRSGAKPGDWIGITGKLGGSIRRHHFDFTPRVKEGLLLSRHSLVRSMID
                     VSDGLFQDLQHILRASGVSAEVDLEAIPVSSDAIVLCHGDTVAALKRALTDGEDFELL
                     LTVSSRQKAVLEPLWKRKFPSVGITWIGRIVKGRPEVQWFSKNKKIRIPNLSCVGYQH
                     F"
     gene            7018..7443
                     /gene="tsaE"
                     /locus_tag="PK590_02340"
     CDS             7018..7443
                     /gene="tsaE"
                     /locus_tag="PK590_02340"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOG23469.1"
                     /translation="MKKKSVITNSSGETIRFAKKFAKELKPGSVLCLEGDLGSGKTTF
                     VKGLAEGLGLKNPSQVKSPTFVLMHVYKARIPLYHFDCYRIDRVDELSNIGVSDFACD
                     PEAITCVEWAEKARELIPPYAVRICFEILGVSKRRLVIG"
     gene            7554..8234
                     /gene="radC"
                     /locus_tag="PK590_02345"
     CDS             7554..8234
                     /gene="radC"
                     /locus_tag="PK590_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010676114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOG23470.1"
                     /translation="MIKSIKEWPENERPREKLLGRGSETLSDAELLAVIFGHGVRGMD
                     AVSFSRVLLSEAGGLRSLLNRPQDFSGKMKGMGRTKVAILCAVRELGLRHLREKITGQ
                     NFVRSPQAVADYLCAALRDRKREVFKVIFLDKSLRILGEKDLFIGTIDEAAVHPREIV
                     KSALEFHASNLVLVHNHPSGKIDPSREDYEITQKIKAACQTVSIRILDHIIVGENQFF
                     SFSERNAL"
     gene            complement(8306..8992)
                     /locus_tag="PK590_02350"
     CDS             complement(8306..8992)
                     /locus_tag="PK590_02350"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOG23471.1"
                     /translation="MQKKLLLFDIDGTLLLTGGAGKIALEEAFAEIFGVSDCWKNLDP
                     HGKTDPAIFDEIAIRELGRVLSEEEFYRLMTRYEDLFGAHIHQSPHFELMPGVIPLLE
                     HLSQNDELFLALGTGNFEGAGRMKLARGGIEHYFRTGGFGNHARERHLILLAGIQQSE
                     KIANQKFDKRQTFVIGDTERDIAAANQIGLRSIAVLTNGRTHEDFRPHSPDHILKDLT
                     DITAFLSCLE"
     gene            9199..>10866
                     /locus_tag="PK590_02355"
     CDS             9199..>10866
                     /locus_tag="PK590_02355"
                     /inference="COORDINATES: protein motif:HMM:NF017043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23472.1"
                     /translation="MLQPNLPNLDTPVKLTPMMEQYRSIKKTLSKDTVLFFRLGDFYE
                     MFFEDAVRASDILNITLTGREGGEAGRVPMCGFPHHAFQGYVRTLLDSNLKVAICEQI
                     GDPEQAKGLVERRITRIITPATYLDDETPQKSLQYMAAILTDKNTAILAYLELSTGEF
                     YVREVLPDRLADELSLLGPREVVIPKSLESRKDLTGFIRETIQATLTVYEDWIFEYED
                     SMRLLVETFKLGSQKAVTFHDHPLAVGATGAILYYLKDHFHSALEHVQKPRLFNVSQF
                     MTLDRQAQKSLELTASLSDRKDYGSLLSVIDRTLTAMGSRILYHWLTHPLLEVREIRR
                     RQDSVRDLIQEGRRTETIAKLLKGVKDMERTLSRLNYGVANARDLVNLRIFLERVPEI
                     RNHLAAFESELIRSLQKNIQPFPAISELIGRAIVDEPPLAVKDGGLIRDGYSGDLDDL
                     RGISKNGKQWILDFQRREIERTGIKSLKIKYSQVLAIPSRLVNLICRRYRKIMSVDRH
                     WQMPRGLLSLNLKNGTKRFPALRIEYKRWNTRSFLKFVRRCLRNCTRF"
BASE COUNT         2651 a         2372 c         3001 g         2842 t
ORIGIN      
        1 tttaaagcgg ggacaagaaa tatgaagaaa aaagggattt ttattacatt tgaaggagcg
       61 gaaggaagcg ggaagagtac acagatcagg gaagcggcga aattctttcg gaagaaaggg
      121 cgggaagtga tccttcttcg tgagcctggc gggacaaaga ttagtgagtt gatccgcaag
      181 attttgctgg atcgcgcaaa tcaggggatg agtcacgcca ccgagctttt tctttatctc
      241 gcggcgcgcg cccaggtcgt ggaggaaaaa attttacccg cgcttcggga aggaaaagtt
      301 gtcatcgcgg accggtttga agattcaacg agggcctatc agggatttgg gcgcggattt
      361 tcgctcgagg cgatcgaaag catgagccgg cttgttcgcg ggacattaat cccggacctt
      421 acgtttgtgc tcgatgttga tattaccaag gggctcgcgc gtggtggccg tcacgaccgg
      481 attgaatggg aggccctgag tttccaccgg aaagtgcgtc agggctattt gacgcttgcg
      541 cgaaaggaac cgggtagaat gctggtgctt gacacggacc agcccgtttc ggttgtcaaa
      601 caatgcgttc tggagaggct acaacgtgta tttggcaaat aaaacccaaa aactggcggc
      661 acggatactg gaatcgcata tggcccggcg aagagtgtcg cacgcctata tttttagtgg
      721 ttctggcgaa gctttggaga tggccgagct agcgggcgag cgtggtagtg ccgatattaa
      781 agaagaattt gcggtggcgt ttgccgcggc gcttctgtcc ggaagtgaaa agcttttcca
      841 agtggcggaa tcttcgatct cggcgcggat ctggaaacga aaccatccgg atgtccgctg
      901 gattggcgaa gatcaggaag aacggtcaat taaagtcgga gaggacagca gtgagcgggg
      961 cacgattcgt gaaattattc attgggcggg aattaaacct tacgaaagcc catggaagct
     1021 ctgcatcgtt ttgaagtcgg agcggctgac ggatgtggcg gcaaatgctt ttttgaaaac
     1081 gctggaggag ccgccgcctc atacggtgtt cctcttgctc gtggagaata aaacacatct
     1141 tctcgagacc atccagtccc ggtgctttga gatcaggata ccggcttccg agagttttgc
     1201 tgtgccacga agtctcgcgg gacttgcgac gattccggtg aaagaaattt tagagtctta
     1261 tccctcgctt tccaaagaag atttgagacg aaaactcgat ggtctgatgc cgttcattag
     1321 ggaaaaaatc acgaatcttg tttcagccga ctcgccggat ctggaccgct ggctggaagc
     1381 gatggatctg gtttatgatt ccaaggcggc cctcggagac aatgtgaatt acaaactcat
     1441 gctgacccga ctcgcgatgc gcctttcaag aatattacca gtagacggaa aaggattaat
     1501 ttgatgacga attattattt gacgacaccc atctattacg tgaacggaaa gcctcatatc
     1561 ggacacgcgt acacgagcat tctttgcgac acgttcgcgc ggttccaccg gatgctcggc
     1621 gaggaggtct tttatctcac gggaacggat gaacatggga tcaaggtgca aaaagcggcc
     1681 ctaagcgagg ggaaaactcc ccaagccttt gtcgatgaga ttatgcccaa atttcgggaa
     1741 ctctggaccc ttctcggagt ccggtatgac tactttattc gcacgacgga tgctgagcat
     1801 aaagcggggg ttcagaaaac cttaagcgcg ctcgaggcgc aaggtgacat ttacaaggcc
     1861 tcttatcatg gctggtattg cacaccgtgc gaatcttttt ggacggaact ccagcttaaa
     1921 gaaggaaagt gcccggattg cggtcgtgcg gttgagcggc ttgaagagga aaattatttt
     1981 tttaagctgt caaagtacca gggatggtta atggattata ttcagactca tccggatttt
     2041 atccggcccg attaccggag aaatgaaatt ctagggtttc tccgcgagcc ccttgaagac
     2101 ctttgcatta ccaggcccaa atcgcgtttg tcgtggggaa ttgaatatcc cggttctcaa
     2161 gaccatgtcg tttatgtctg gtttgacgcg ttacttaatt atttgaccgc tctcggttat
     2221 cccgcggaag aatcaaagtt taaaaaatac tggccggtgg attgtcacat gcttggaaag
     2281 gatatcttgc gccagcacgc ggtgtactgg ccgatcatgc tccgggccat gggtgtggaa
     2341 atgcccaaac tggtgctggc ccacgggtgg tggaaaatcg gaggagagaa aatgtcaaag
     2401 tctctcggaa atatcgtgga tccggttgcg atcgtaaaaa aatacggtag cgacgcgttc
     2461 cggtattttt tgatgcgtga agtcactctg ggactcgacg ggacgtattc cgaggatctc
     2521 ctgaaagaac gatacacgag tgatctcgcg aacgatattg gcaatttatg gcaccggacg
     2581 gcatccatga tggagaagta ttttgggggg aaagtcccaa cgagctctca gggtgcggaa
     2641 aaaactccgc tttggcaaaa tgtcatcggg ttgtgggaca aggtttgtcg ggatatgctg
     2701 gcttatgatc cgagagaatc gctcgcgaag atctggaccg ttgtcaccca ggctaatcag
     2761 tttgtcgagg agaaaaaacc ttgggtgctc gcgaagcacg aggatcagaa acaggagctt
     2821 gccgatacac tttatgtgtt ggctgaaacg gtggcgcata tcggtgtttt gcttcaagcc
     2881 tttctgccgg aaacctcgcg gaaaataaca agtcgcttga agctctccga ccctttcgcg
     2941 attaaagacg aatccgcttt cagaaaaccg cttgtgaaag cgggaacggt gattgaaaaa
     3001 ggagatcctc ttttcccccg tcttgaagac aagatcgcgc catgctgacg gacacgcatc
     3061 ttcatctcca tttttcccag tatgatctgg atcgcgatga ggtgatccgc cgcgcgattg
     3121 ccgggggagt ggggctcatg attaatgtcg gaacggatct ggaagattcc cgcaaagccg
     3181 tccaggttgc cgaaacctat cccgaggtgt atgccgcggt aggctatcat cctcatgaaa
     3241 gcaagaacgc aagtcgggaa gcgctcgcgg agttggaaaa acttatcgcg catccccgag
     3301 tggtcggggt gggagaggtc gggctcgatt attttcacaa tcattctccc caggagaccc
     3361 agcgggagat atttcgagtg tttctgtcct ggtatcgccg acatcaaaaa ccgatcatac
     3421 tccactgtcg tgaagcttac gaagatatgc tccggttgct gaaagatgaa ttggagcttc
     3481 cgtgtcgcgg agtgatccat tgctactcgt cggattcgga aatgatgttt aaattcctgg
     3541 agctcgggtt ttacgtcgcg tttggaggtg ctttaactta taaaaaaaat gaaaccctga
     3601 gggaagcctg tcgccaatgt cccctggatc gattgctgat tgaaactgac gcgccttatc
     3661 ttgcccctca atcaagacgg ggtgagagga atgagcccct ttttttggta gaaacggcta
     3721 aatttatcgc gtcgcttcgc ggttgcgatc cggagaagct ttttacggaa acgacggcca
     3781 acgcccgaag actttttgat ttatgctaaa ccgggttttt cttttcttaa ggcacgaacg
     3841 ggtatacttg ctcctcatcg tgttgatcgg tgttttttac gcggcgatta cgacggggac
     3901 ggggaggaaa attcgtgcgt cgcaggcggt tccatcacag gctatggagg aattcaaagc
     3961 cgcggagcgc caatttaatg aacaatcatt ctcagggtgg gcgtccgagg agtttaggaa
     4021 tcagaaccct cttttaaccc gctcttttga attttttact cttttttttc tgctcgccat
     4081 tgtcgctggg ctggggattg attttatcct gcttgtccgt ccttcgttgc gcaagaagct
     4141 tctgtatcgc cctgacccgc ccccggttcg tgaatggccc ttttccattc tgctcaggac
     4201 cgtggtcctt tttattacgt gggggattgt tctcagcgtg gcgattggtt tgctggaatc
     4261 gtttttttta aaaacgggtt cggaaaacct gctcatgatt cttcatacgt taatactaga
     4321 tgttctcggc cttatacttg tagtttactt tttaaagaaa caggggagtt cctggcgcga
     4381 acttggtttt aatattccgc agggaaaggt attcagggaa atgggaattg gtatcaccgg
     4441 gtatcttggg actctgcctt tgtttgttct ggtgctgatg gcgctgatgt atttttccag
     4501 tctgttcagc tatgagccgc cgccgcatcc ccttgtgaat gtttttgtgg aagaagaaaa
     4561 gcggcaaccg tttctggtta ttttctcaat attgtttggg gcggtgatcg ggcctgtttt
     4621 tgaggaaatt tttttccggg gattctgtta tcccattctt aaaggaaaga tcggaaaggt
     4681 gggagcgatg gtggtgagct cggctttctt cgcgtcgatt catcatactg gatttgtttt
     4741 ttggcctatt tttattctcg gaatggcgtt ggcatatctt tatgaaacga ggcgttcgtt
     4801 gctagcgccg atgacgcttc atgttgttca taattcgcta ttgatcggat acttttttct
     4861 cgcaaaacag gtgatcagtt caagccaccc actttgataa aggaaacagg catgcaaaac
     4921 aaaaaggttc cattcgcgcc aatcaagttt tcacgacatg tgctttatct tttggaccag
     4981 cggaaacttc cgattcggga agtctgggtt gcttgtcgga attcccgaat ggtctggaag
     5041 gccatccggg acatgattgt tcgcggggcg cccgcgattg gtattgccgc ggggtatggt
     5101 ctctatctgg gggttcggca tttccaggga gaccagaagg gattttggga aactctgcgc
     5161 gaccagggac gtttcctgga ctcggcccgg cccaccgccc gtaatcttgc ttgcgcgctg
     5221 gagagaatca tgaaagccgt tcattgcgcg ggggatcgaa gtgttgagga gctgaaaaaa
     5281 ctggtgctgg cggaagcgat ccgtattcat catgaagaca ttgatctttg ccgaaaaatc
     5341 ggggtgttcg gatccgaact gttcaagaag ggggacgtcc tcctgaccca ttgtaatgcc
     5401 ggagggctcg caacttccgg gtatggaact gccctcgcgg ttttttatgc gttgaaggag
     5461 aaaaaaatac cgttttctgt ttttgtggac gagacacgac ctctgatgca gggggcgcgc
     5521 ctgacggcct gggagcttca aaaaagcaaa attcccgcta ccctgatatg cgacaatatg
     5581 gcggccagcc tgatgcgtgc gggaaaaatt catggcgtcg tggtgggcgc agaccggatc
     5641 gcgatgaatg gagacaccgc aaacaaaatc ggaacttatg gtgtcgctgt gctggcgaag
     5701 gttcacggga ttccttttta cattgccgcg ccgagtacaa cttttgatct gaaggcaaaa
     5761 accggggagg atattcctat tgaagaacgg ccgcaccacg aggtgcgcga aattcaggga
     5821 aggccgcttg cgccgaaggg aatgaaagtg tttaatccgt catttgatgt gacgccgcac
     5881 ggctacattt ccggcattat cacggaaatt ggggtcttgc gcccgccatt cacaaaatcg
     5941 atacgacgaa ttggcacttt acttgaaaaa tcatagcgcg ggcgtggtgg ccggtttgac
     6001 tgaggggatg gggatgaacg aaattgattt tattgaacac ctgaggcgga aaatttccag
     6061 atcacgcgcg gtgtcggttg gaatcggcga tgatgcggcg gttttggcgc cgctacgcag
     6121 gggaaggctt gtcatttcca cggatatgct ggttgaaaac gttgatttca gaataaaaaa
     6181 agacggtcaa cgtaaagcac ccgacgattt tttgacacct gaacaaattg gccggaaggc
     6241 gctagcggtc aatttgagtg atatggcggc gatgggggcg aaaccgctgg cgtttgtgtt
     6301 gacgatcgga aaaccgacgt atatcagcac aaaatggctg gatcgttttt ataacggaat
     6361 gatgaggttt gcccgagagt atggtgttaa ttgtatagga ggagattttt cacgagctcg
     6421 agaattttcg gtcagtgtga ccattctggg agtcgccgag aagccgatac tccggtcggg
     6481 cgcaaagccc ggagactgga tcggaatcac gggaaagctg ggcgggtcga tccgccgaca
     6541 tcattttgat ttcacgcccc gagtgaaaga agggttactg ctttcccgcc attctcttgt
     6601 tcgtagtatg attgatgttt cggatggtct ttttcaggat cttcagcaca ttttgcgcgc
     6661 gtcgggtgtc tcggctgagg tggatcttga agcgatccct gtctcttcag acgcaatcgt
     6721 tctgtgtcac ggggatacgg ttgccgcgct taaacgggcg ttgacggatg gtgaggattt
     6781 tgagctgttg ttaaccgtgt catcgaggca aaaggcggtt cttgagcccc tatggaaacg
     6841 aaagtttcct tcggtcggga taacctggat agggcggatc gtaaaaggca ggcctgaggt
     6901 tcaatggttt tcaaaaaata aaaaaattcg gatccccaat ttgtcctgcg ttggctatca
     6961 gcatttttaa aacaaaaaga ttttttcgga agtctctaaa agtccgtcct gtgtgatatg
     7021 aaaaaaaaat cagttattac gaactcttcg ggtgaaacga ttcgcttcgc gaaaaaattt
     7081 gcgaaagaat tgaagcccgg cagtgtgctt tgtctggaag gggatttggg gagcgggaaa
     7141 acgacttttg tgaaaggact ggctgaaggt ttggggctca agaatccttc tcaggtaaaa
     7201 agcccgacct ttgtcctgat gcatgtttat aaggcgcgga tccctttata tcactttgac
     7261 tgctaccgta tcgacagagt cgatgagtta tcgaatatcg gagtttctga ttttgcgtgc
     7321 gatcctgagg ctattacttg tgtcgaatgg gctgaaaagg cgcgcgaatt aattcctcct
     7381 tacgccgtcc gaatttgttt cgaaatcctg ggcgtttcta agcggcgact tgtcattggc
     7441 tgattaaaac tctccgaaga aagaagaaaa cttgttttta aatatgcgga ataataaaat
     7501 aaaaaatgca aataaagcat tgacaaagac actgcaggct gtagtatggc gacatgatca
     7561 agagcataaa ggaatggcct gaaaatgaaa ggccgagaga aaagttgctg ggcaggggga
     7621 gtgagacgtt atcggatgct gagttgctgg cggtgatttt tgggcacggg gttcgtggca
     7681 tggatgcggt cagtttttcg cgtgtcttgc tcagtgaggc gggcgggctg aggtcgttgc
     7741 ttaatcggcc gcaggatttt tcaggaaaga tgaaagggat ggggcggacc aaggttgcca
     7801 ttctttgcgc cgtccgtgag cttggattaa gacatctgcg ggaaaagata accgggcaga
     7861 acttcgtccg aagtccccaa gcggtggcgg attatctttg cgcggcgctc cgggatcgga
     7921 aacgcgaggt ttttaaggtg atttttctcg ataaatctct gagaattctg ggggaaaagg
     7981 acctttttat cggaacgatt gatgaggcgg ccgttcatcc gcgagaaatt gtaaaatccg
     8041 ccctggaatt tcatgcttcc aacctcgttt tggtccataa ccatccctcg gggaaaatcg
     8101 atcccagccg ggaggattac gagatcacgc aaaaaataaa agctgcctgc cagactgttt
     8161 cgattcgaat actggatcac atcattgttg gagaaaatca atttttcagt ttttctgaac
     8221 gaaatgcttt gtgaggacaa acttggcgtt cgtatccttc caaagaataa aaaatggcag
     8281 ggcttttttg atttgtgttt ctcttttatt ccagacagga gagaaaagcg gtgatgtcgg
     8341 taagatcttt tagaatgtga tcaggcgagt gagggcgaaa atcctcatgt gttcgaccgt
     8401 tggtaaggac tgcgatgctg cgcaatccta tttgattggc ggccgcgatg tcacgttcgg
     8461 tatctccgat gacaaaggtt tgtcgcttgt cgaatttctg gttcgcgatt ttttcggatt
     8521 gctggatacc tgccagaagg atcagatgtc gctctctcgc gtggtttcca aaaccccctg
     8581 tccgaaaata atgttcaatt ccaccccggg cgagtttcat tcgccccgcg ccctcaaagt
     8641 taccggtacc cagagccaaa aacagctcat cgttttggga gaggtgttcc aagagaggaa
     8701 tgacacccgg catcaattca aaatgagggg actggtggat gtgagctcca aagagatcct
     8761 cgtatcgcgt catgaggcgg taaaattctt cctcggaaag tacgcgacca agctccctta
     8821 tggcgatttc atcaaagatt gccgggtctg ttttaccgtg agggtccaga tttttccagc
     8881 agtcggaaac gccaaatatc tccgcgaaag cctcttcgag agctattttg cccgctcctc
     8941 ctgtgagtaa aagcgtaccg tcaatatcga ataacaacag ttttttttgc atgcgagatt
     9001 ttatcatgcc atgcaatggt cggctttttt gcgggaaagc agtctcagaa cagctaaaag
     9061 cagttaaaag cgctttcatg attattttgc atccgctata ctaatgcccg cgatggctgt
     9121 gttttgacga ttgattttgt gaattgaggt tttatcgcat ttttgaaccc gtttatgatt
     9181 ttcaggagtg agctgactgt gttacagccg aaccttccta atcttgatac tccggtcaaa
     9241 ttgacgccga tgatggagca gtaccgatcc atcaaaaaga ctctttctaa ggacacggtc
     9301 cttttttttc gactgggaga tttttatgaa atgttttttg aagatgctgt gcgcgcttca
     9361 gacattctta atatcaccct gacgggtcga gaaggcggtg aggcgggccg ggtccccatg
     9421 tgcggatttc ctcatcatgc ctttcagggc tatgtcagga ccttgctcga tagtaacctc
     9481 aaggtcgcga tctgcgaaca aatcggagat cctgaacagg ccaaggggct tgtggaacgc
     9541 aggataacgc ggatcatcac acccgccact tatctggatg acgaaacacc ccagaagagc
     9601 ttgcaatata tggccgctat tcttacggac aaaaacaccg cgatcctggc ctatcttgag
     9661 cttagtaccg gcgagttcta tgtgcgcgag gtattgccag accggttggc tgatgaatta
     9721 tcccttctgg gaccgcgtga agtcgtgata cccaaatctc tggaatctcg caaagatctg
     9781 acgggtttca ttcgagaaac gatccaggcg actttgacag tctacgaaga ctggattttt
     9841 gaatatgaag attcgatgag gcttttggta gagactttta aattaggatc ccagaaagcg
     9901 gtgacttttc acgatcatcc tttggcggtt ggcgctaccg gcgcgattct ctactacctt
     9961 aaggaccatt ttcacagcgc cctcgagcat gtgcagaagc cgcgcctttt taacgtaagt
    10021 cagtttatga cccttgatcg acaggctcag aaaagcctgg aactcaccgc gtccctcagc
    10081 gaccggaaag actatggatc cctgctttcg gtgattgatc gaaccctgac ggccatggga
    10141 tcgcgtatcc tttatcactg gctcactcat cctttacttg aggttcgtga aattcgaaga
    10201 cggcaggatt ccgtcagaga cttgattcag gaaggacgcc ggactgaaac aatcgcgaag
    10261 ctcctcaagg gagttaagga catggagaga actctcagcc ggctgaatta cggtgtggcg
    10321 aacgcaaggg atcttgtaaa cctccggatt tttcttgaaa gagttccgga gattcgcaac
    10381 catttggcgg cttttgagag tgaattgatt cgttctctcc agaaaaatat ccagcccttt
    10441 cccgcgatca gtgaattaat tggccgcgcg atcgttgacg aaccgcctct tgccgtgaag
    10501 gatgggggac tgattcggga cggttattca ggagatctcg atgatcttcg cggaatctca
    10561 aaaaatggga aacagtggat ccttgatttt caacgtcggg agatcgaacg caccggcatt
    10621 aagtcgctaa agattaaata ttcgcaggtt ttggctatac catcgagatt agtaaatctt
    10681 atttgtcgca ggtaccggaa aattatgtcc gtcgacagac actggcaaat gccgagaggt
    10741 ttgttgtccc tgaacttaaa gaatgggacg aaaagatttc cggcgctcag gatagaatac
    10801 aagcgttgga ataccagatc tttcttgaaa ttcgtcagaa ggtgcttgag gaactgcacg
    10861 cgcttc
//
LOCUS       DAOSIO010000035        10765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_6013_length_10765_cov_5.146032, whole genome shotgun sequence.
ACCESSION   DAOSIO010000035 DAOSIO010000000
VERSION     DAOSIO010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 10765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10765
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6013_length_10765_cov_5.146032"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PK590_02360"
     CDS             complement(<1..580)
                     /locus_tag="PK590_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23473.1"
                     /translation="MTNTKKTVFVLAIAVFFLSPLKMFAQELVSEDVLERAVQDPFPS
                     GLLAISDYKYPVFLYAPRGYRADRSYPMIMVAPSEGVGGEEGIAYWKSIADQNMVFVL
                     STAGVWVESDDVPTNLDTWLLQVKKDVQERFPVNDKKIYVAGKNTGAHYAAYLATNYP
                     QEFAGAVLLGQAWAGHFSRLIHSSSNLSKQQLF"
     gene            complement(840..1232)
                     /locus_tag="PK590_02365"
     CDS             complement(840..1232)
                     /locus_tag="PK590_02365"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOG23474.1"
                     /translation="MLKKVFLFLAILLAAGLILSFIYLRSFSRYHELKQEEERLVQRI
                     QEIDSQIKSLTDEKYLLQNDIEYLEKVVREELGFVKPGEIIYELVTAKNSAGPVVVVD
                     KSPAPSTGVDASTATTSTAISGKKTKKH"
     gene            complement(1226..2542)
                     /gene="eno"
                     /locus_tag="PK590_02370"
     CDS             complement(1226..2542)
                     /gene="eno"
                     /locus_tag="PK590_02370"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOG23475.1"
                     /translation="MANTTIVNVNAREILDSRGNPTVEVDVVLQDGVLGRAAVPSGAS
                     TGEHEAVELRDGDKARYLGKGVLKAVEHVNTEIARELKGKDASDQSGIDKRMIELDGT
                     PNKARLGANAILGVSLACAHATCVSRKMPLYELIGGPSANLLPVPMMNIINGGVHADN
                     NVDLQEFMIMPFGAPTFRDALRMGAEVFHNLKKILHAKKLSTSVGDEGGFAPDLKSNH
                     EALDVIMEAIEKAGYKAGTDVKIALDPASSSFYGQDKLGGNQNPGKYVLCAEAQPVKS
                     AEDMVNFYEALAGKYPIFSIEDGLAENDWDGWKKLTDRIGAKVQLVGDDLFVTNTKRL
                     AMGIEKKTANSILIKVNQIGTLTETIEAVRMAHQHGYTAVMSHRSGETEDTTIADLAV
                     ALSTGQIKTGSTSRTDRICKYNRLLRIEEALGSKAVYAGLKLGKAC"
     gene            complement(2734..3318)
                     /gene="purN"
                     /locus_tag="PK590_02375"
     CDS             complement(2734..3318)
                     /gene="purN"
                     /locus_tag="PK590_02375"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006694314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOG23476.1"
                     /translation="MKRLAFLVSGNGSNMANLIRRIKKGEIRRAEAALVISNKPDVKA
                     LGRALEFGVPSVVISHGDYDSRESFDEALSECLDRHKIDLVCLCGFMRILTEGFVRKY
                     YGRLVNIHPSFLPDFPGSHAIRDAWNARVKETGVTVHFVDAGVDTGTVILQKKVPVLS
                     GDTFETLETRIHETEYDVYPEALNLVLEGKVSAR"
     gene            complement(3358..4050)
                     /gene="rsmI"
                     /locus_tag="PK590_02380"
     CDS             complement(3358..4050)
                     /gene="rsmI"
                     /locus_tag="PK590_02380"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOG23477.1"
                     /translation="MLSFVATPIGNLGDITIRALEVLRAADFIACEDTRETVKILKHY
                     EIPEKQFLSYHEHSPEIRVHEILAQVRQGKRVAVVTDGGMPSISDPGFTLVREAIREG
                     VPFEVLPGPSAVLTALVASGLAVDEFSFLGFLPNKSASRKKRLAEVADREETLIFFES
                     PYRLPSALRDMFEVFGDREAVVAREMTKKFEERLRGRLSALVRDVESRPRKGEVVILV
                     SGKNRKRVLSES"
     gene            complement(4094..5101)
                     /locus_tag="PK590_02385"
     CDS             complement(4094..5101)
                     /locus_tag="PK590_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOG23478.1"
                     /translation="MKTTLTLETIYAPIAAGIQRVPAVIKETLRTPSRQIEDVVQYFF
                     SKHGKLLRPALVFLGGGMARSLAPAAEVISERQETSEVYLAAATEIFHAATLIHDDII
                     DSAPIRRGLPSLHVKWGPQTAVLAGDFFHDKAVVTIFRHGNERIIPLFLKTAGEICEG
                     EVQELSEKNNINLSESTHFEILGKKTASLLACSLQTGALIWGMDETRVDALGRYGHAF
                     GMAFQIIDDYLDFTGRESEFGKTLGADLEEGVYTLPVIHCLASSDRDKVVRILHARNH
                     AKRFPMLKKLLQKSGALDYSLEKAQGFIRQAKQALAPFPESEFKNSLCELADYVIQRE
                     R"
     gene            complement(5142..6038)
                     /gene="rsmA"
                     /locus_tag="PK590_02390"
     CDS             complement(5142..6038)
                     /gene="rsmA"
                     /locus_tag="PK590_02390"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOG23479.1"
                     /translation="MGEDLPMLSQVEILKKYHIRVRGHLGQHLLMDPNMVRKIVDGLG
                     LDKAEPVLEIGPGLGSLTVELLSRGHPVLAIEKDKRFVEILQNEIVPAFPGQLSIVNR
                     DILECDLSEMIRGREPEALHDFREKMSLTKSGFKVVSNLPYYISTPILFHLIAHRACF
                     HSAVLMLQKEVAARMTAKPGTPDYGRLSVSASIFGNTETLFDVSPKCFLPAPEVTSRV
                     IRYTFVPDEGLTEDESLPEIIRIAFSERRKTLLALLLKYWKPKKNRMELESIFAQLGF
                     ALNARGEELATGQFRKLAEALK"
     gene            complement(6070..7089)
                     /gene="pdxA"
                     /locus_tag="PK590_02395"
     CDS             complement(6070..7089)
                     /gene="pdxA"
                     /locus_tag="PK590_02395"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOG23480.1"
                     /translation="MTKHQLLRHPATAVRAPVIAITMGDPGGIGPEVVVKCLKRLKRG
                     PRCRYVVIGSQAVFSFLNKQTSLDSSFQRFPSDGRVLQKGIVYFWDIGGAKFDKSRET
                     VANGQMAIRAISIASDLAKEGSVQAIVTAPINKVSARLAQKGFVGHTEFFAQAAGVKH
                     YAMMFVGASLKLTLATIHVPLKRVSGLLSSDLIFEKIILTADALKNDFKIKNPKIAVC
                     AFNPHGKECGGGEEITVISPAIRRAQRKGILASGPYPSDQIFYSARRGAFDALITMYH
                     DQALGPFKMIHFHDGVNMTLGLPYIRTSPDHGTAYDIAYQGKADPSSMMAALKLAETL
                     VLNRS"
     gene            complement(7111..8079)
                     /locus_tag="PK590_02400"
     CDS             complement(7111..8079)
                     /locus_tag="PK590_02400"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOG23481.1"
                     /translation="MKKFLLILCLFASLGVPLGGAQEELLDRVIAVVNDEIITQSELD
                     SILRPLYEDYKEQYSGDELVDFVKEARQKILNQLIEDHLVYQEAVSQEIVITEDEIDR
                     ELEDLGKRMEGYDEINSMLEREGMTMKALREKIKKQLMIRRLQDIQIRSRVVISPAEI
                     ESFYKENPEKFQRTARVKIKSLTVRKSAESREQGTMDEAARDKIKSFYLKIKQGDNFD
                     DLVRKYSEDSRARQEKPADWIEKGSMIESVDEAIFSTPQGQLTDIVESPIGFHIFRIE
                     EKEDARQVTFEESREQISGYLFRAKSNERFKEWTEQLKRAAYISIR"
     gene            complement(8153..10066)
                     /gene="mfd"
                     /locus_tag="PK590_02405"
     CDS             complement(8153..10066)
                     /gene="mfd"
                     /locus_tag="PK590_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOG23482.1"
                     /translation="MNIKHFKTGETIRFEDLESTLQELRYVRKNLVELPGEYSIRGGT
                     VDVYPVTYRMPVRLDFRGNTLYQIRDFSPPEGKSFTLFEEVFLIPLSGHYERKVRRLE
                     EKFGAFEPVTGMGELHRGDFVVHFHYGIGRFLGSKMIRLEGKKERCFAIEYAEGEILY
                     LSMKEPIERYIGGSSAAPKLTRLNTKEWERIKQRTRNALHKVAGDLLELQARRNVQQG
                     FAFPRDHEWQKIFEDEFPFDETEGQKRAIDEVKRDMESSKPMDRLLCGDVGYGKTEVA
                     IRSAFKAVTGGKQVAFLVPTTILAEQHYILLKNRVKTFPVTVEILSRFQSPSEQKAVV
                     RSLKTGATDIVVGTHRLLSGDIKFKELGLVVIDEEQRFGVRHKESLKKMRSQIDVLTL
                     TATPIPRTLHMALLGVRDMSVIDTPPRNRLPIKTFVTESHDLVIRQAIEREVERGGQV
                     YFVHNRVENIEKVHEKLRKILPNIQFGIAHGQMPVEMLEKVMKQFMKGAIHCLISTNI
                     IEAGIDIPNANTLIVNRADCFGLADLYQLRGRVGRFKEMRQAYAYFLTPRGSMTPDAA
                     KRLAAIERFIELGSGFKIAMEDLEIRGAGNLLGHEQSGFIHAVGFDLYCRMLKKAVEE
                     HRSGNPEPETQSS"
     gene            complement(10493..>10765)
                     /locus_tag="PK590_02410"
     CDS             complement(10493..>10765)
                     /locus_tag="PK590_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG23483.1"
                     /translation="RRDLFLSLLGKIDCFTTTKPGGAFYLFVNIKKTGLSAFEVSKRL
                     LEEAHVAVIPGEPFGSAEHIRLSYAVSEKEIYSAVERMANCFKQKK"
BASE COUNT         2708 a         2947 c         2361 g         2749 t
ORIGIN      
        1 aaaaaagctg ttgcttagac aaattggaac tcgaatgaat tagccgcgaa aaatgtcccg
       61 cccatgcctg ccccaacaaa accgcccccg cgaactcctg gggataattt gtcgccaaat
      121 aggccgcgta atgagctccc gtattttttc ccgcgacata gattttcttg tcattcaccg
      181 ggaatctctc ctggacatct tttttcacct gtaacaacca tgtatccaga ttggtgggca
      241 catcgtcgct ttcaacccac acgcctgccg tcgaaaggac aaacaccatg ttttgatccg
      301 caatgctttt ccagtaagca atcccttcct ctccccccac cccttcactc ggagcaacca
      361 taatcatggg atagcttcga tctgccctgt aacccctcgg ggcatacaag aaaaccggat
      421 atttataatc cgaaatcgcc aaaagaccgg acggaaacgg atcctgaacc gccctttcca
      481 aaacatcctc gctcaccaac tcttgagcga acatcttgag agggctcaga aaaaaaacag
      541 caattgcaag gacaaagacg gttttctttg tatttgtcat aagaacacct ttggtctcgc
      601 gagggttaaa tgatgtaagt gactgaaagt aaagaggttg ttattataat gattcgcctt
      661 atgttgtcaa gggcatccag agagataaat tttcctctcc ctcagtgtga gcgggtcgag
      721 aaaactccac aatacctcac gttttcttcg gtcggaagct aatgtatctt tggctatatc
      781 aataggttac gacgatccag acagggaacg tccgtatctt ttccggaatt accgaggggt
      841 tagtgtttct ttgttttctt cccagaaatc gcggtggacg tggtagcggt ggaggcatct
      901 actcctgttg aaggcgccgg agacttatcc acaaccacca caggccccgc cgagtttttt
      961 gccgtcacga gttcgtagat aatctcgccg ggtttaacaa atccgagctc ttcgcgcaca
     1021 accttctcaa gatactcaat atcattctgg agaagatatt tttcatccgt gagggacttg
     1081 atttgggaat caatttcctg aattcgctgt accagtctct cctcttcctg ttttaactcg
     1141 tgatacctgg aaaaagaccg cagatagata aaagaaagta tcagccctgc cgccaacagg
     1201 atcgcgagaa acagaaagac cttttttagc atgctttccc aagcttaagc cccgcgtaca
     1261 cggctttgga tcccagagcc tcctcgatgc gaagcaatcg attgtattta caaatcctgt
     1321 ccgtgcggga agttgacccg gttttgatct gacccgtgct aagcgccacc gcgagatccg
     1381 cgatcgtcgt atcttcggtc tcaccactgc ggtggctcat gacagccgtg taaccgtgtt
     1441 ggtgcgccat gcggacagcc tcaatcgttt ccgtgagtgt tccgatctga ttgaccttaa
     1501 ttaaaataga attggccgtt tttttctcaa tccccatcgc gagccgcttc gtattagtca
     1561 caaaaagatc gtctcccacg agttgaacct tggccccgat ccgatccgtc agttttttcc
     1621 acccatccca gtcattctcg gccaaaccat cctcgatcga aaaaatcggg tatttgcccg
     1681 cgagcgcttc ataaaaattc accatatcct cggcagattt tacgggttgg gcctccgcgc
     1741 acagaacgta tttcccggga ttctgattcc cgcccaattt gtcctgcccg taaaaactcg
     1801 aactggccgg atcaagagcg attttaacgt ctgttcccgc cttatagccg gctttttcaa
     1861 tcgcttccat aatcacgtcc agcgcctcat gattagactt aagatccggg gcgaatcccc
     1921 cttcatcccc aaccgatgtc gacagcttct tggcatgaag aatttttttc aggttatgaa
     1981 acacttccgc ccccatccgt aaagcgtccc gaaacgtcgg agcgccaaaa ggcatgatca
     2041 tgaactcctg aaggtcaaca ttgttgtccg cgtgaactcc gccgttaatg atattcatca
     2101 tcggtaccgg caagaggttc gccgaagggc cgccgattaa ttcataaaga ggcatctttc
     2161 tcgaaacaca agtcgcgtga gcgcaggcaa gcgaaactcc cagaatcgcg tttgctccca
     2221 gccgcgcctt attcggagtt ccgtcgagtt ctatcatccg cttgtcaatt cccgattgat
     2281 ccgaagcgtc ttttcccttc agctcccggg caatttccgt gttcacgtgt tccacggcct
     2341 tgagaactcc cttgccaaga taacgcgcct tatcgccgtc acgaagttct accgcttcat
     2401 gttctcccgt ggaagcaccg ctggggacag ccgcacggcc aagaaccccg tcttgtaaaa
     2461 ccacgtccac ttcaaccgtc gggttacccc gggaatcaag aatttctcgc gcgttaacgt
     2521 tcacaatcgt cgtatttgcc attggatgct cctttattgt tgggattgcc agagaaaaaa
     2581 agacaatact tcagatgctt ttcccgatcc gtcacacaac acccgacaga tccttctctt
     2641 ccaaagggaa agagtttttc cttctaacgg ggaagacgtc cgaaacaggc ggtgattata
     2701 acaaatcccg cagaggtgtc tacagtgaga aacttagcgg gcgctcactt ttccttctaa
     2761 cacaaggtta agcgcttccg gataaacgtc atattcagtt tcatggattc gagtttcaag
     2821 cgtttcaaac gtgtcgccgg aaaggaccgg gacttttttc tggaggatca ccgttccggt
     2881 atccactccc gcatcgacaa agtggaccgt cacacccgtt tctttgaccc tggcgttcca
     2941 cgcgtccctg atggcgtggc tcccggggaa atccgggaga aatgagggat gtatgttaac
     3001 gagacggccg taatattttc gaacaaatcc ttccgtcaga atccgcataa acccgcagag
     3061 gcagacgaga tctatcttgt gccgatcgag acattcggac aaagcttcgt caaaagattc
     3121 ccggctatcg taatccccgt gagaaatcac aaccgaaggc accccaaact ccagggcccg
     3181 ccccagcgcc ttcacgtccg gtttgtttga aatcacaagc gccgcttcag ccctccgaat
     3241 ttctcctttt ttaattcgcc ggatcaaatt ggccatattc gatccgtttc ccgaaacaag
     3301 aaaagccaac cttttcatat ccattcacct tcttaaagat tcacaagaca tccgacgcta
     3361 ggattcggaa agcacgcgct tgcggttttt cccgctgacg agaatgacca cttctccctt
     3421 tcggggccga ctctcaacgt cccgcacaag cgcggacaac cgtccacgca accgttcttc
     3481 aaattttttg gtcatttcgc gcgcgacgac cgcctcccga tccccaaaaa cttcaaacat
     3541 atccctgagc gccgagggaa gtcgatacgg cgattcaaaa aagataagcg tctcttcccg
     3601 atccgcgact tcggcaagcc gcttctttcg gctggcactt ttattcggca aaaaacccag
     3661 gaatgaaaac tcatccaccg cgagcccgct ggccactagc gccgtcaaca cggccgaagg
     3721 tcccggcaaa acctcaaacg gcacaccttc gcgaattgcc tctcgaacca gcgtgaaacc
     3781 gggatccgaa atcgacggca ttccaccatc cgtcacaacc gccacgcgct tcccctgccg
     3841 aacttgcgcg agaatttcgt ggacacggat ttcaggactg tgctcatgat aactgagaaa
     3901 ctgcttctcc ggaatctcgt aatgcttcag aattttgacc gtctcacgcg tgtcttcaca
     3961 ggcgataaaa tccgccgccc tcaaaacctc aagggcccgg atcgtaatat cgccaaggtt
     4021 cccgatggga gtcgcaacaa aactcaacat atcaaaaggt ttaacatttc gtaatgcctt
     4081 gagccttcat cgcttaccgt tctctttgga tcacgtaatc cgccagctca caaagactat
     4141 ttttaaattc agattccgga aatggcgcca acgcctgttt tgcctgacga ataaaaccct
     4201 gggctttttc caacgaataa tccagcgctc cgcttttctg gagaagcttt ttcaacatgg
     4261 ggaaacgctt cgcgtgatta cgcgcgtgca gaatacggac gactttgtcg cgatctgaag
     4321 aagcaagaca gtggataaca ggtaatgtat agaccccttc ttcaagatcg gccccgagtg
     4381 ttttcccgaa ttcactttcc cgacccgtaa aatccaggta atcatcgatg atctggaaag
     4441 ccatcccgaa cgcatgcccg tacctgccaa gggcatccac tcgtgtttcg tccattcccc
     4501 aaatgagcgc tccggtttgt aatgagcagg caagcaggga agctgttttt tttcctaaaa
     4561 tttcaaagtg cgttgattcg ctcaaattaa tattattctt ttcactcagc tcctggactt
     4621 ccccttcaca aatctcaccg gccgttttca aaaaaagcgg aatgatgcgt tcgtttccgt
     4681 gacgaaaaat cgtgacaacg gccttgtcat gaaaaaaatc accggccaaa accgccgtct
     4741 gaggccccca cttcacgtga agcgatggca gccctcttcg aatcggggcg gaatcaatga
     4801 tgtcatcgtg aatcagcgtg gcagcgtgaa aaatctcggt cgcggccgca agatagactt
     4861 ccgaggtctc ctgacgctcg gaaatcactt cagctgccgg cgccaaagaa cgggccattc
     4921 ctccgcctaa aaataccagc gccgggcgga gcagttttcc gtgtttggaa aaaaaatact
     4981 gcaccacgtc ttcaatttgc cggctcgggg tgcggagcgt ttctttgatc accgcgggaa
     5041 ctcgctgaat ccccgccgca atcggcgcgt aaattgtttc gagggtcaag gtggtcttca
     5101 tggtccgggt attataacgg aaaaaggaaa tctatcccct gctatttcaa ggcctcggcc
     5161 agctttctaa actgccctgt ggcaagttct tctccccgcg cattcaaagc gaaaccaagc
     5221 tgggcgaaaa tcgattcgag ttccatccgg ttttttttcg gtttccaata tttcaaaaga
     5281 agcgctaaaa gggtttttcg ccgctcgcta aaagcaatcc ggattatttc aggcaaggat
     5341 tcatcttccg tcaaaccttc gtccggaaca aacgtgtagc gaatcaccct cgatgtaacc
     5401 tcaggcgcgg ggagaaaaca tttcggggag acatcaaaca gggtttcggt gtttccaaaa
     5461 atcgaggcgg aaacgctcaa ccgaccgtaa tccggggtcc cgggctttgc cgtcatcctc
     5521 gccgccactt ctttctggag catcaaaacg gcagagtgaa aacaggcgcg atgcgcgatc
     5581 aaatggaaaa ggatgggcgt tgagatgtaa tagggaagat tgctgaccac cttgaacccg
     5641 gattttgtca acgacatctt ttccctgaaa tcgtggagag cctcgggctc ccttcctctg
     5701 atcatttcgg acaagtcaca ctcgagaatg tcccggttca caatcgacag ttgtccggga
     5761 aaggctggca caatttcatt ttgaaggatt tcaacaaatc ttttatcttt ttctatcgcc
     5821 aataccggat gcccacgtga tagcagttca acggtcaagg aacccagccc tggcccaatt
     5881 tctagaaccg gttctgcttt gtcaagccca agaccatcca caatctttcg caccatattc
     5941 gggtccatca aaagatgctg gccgagatgt ccacgaaccc gaatatgata ttttttcaga
     6001 atctccacct ggctcagcat cgggagatct tctcccatcg gggtaattga tcatggcgca
     6061 tcatgaccat cagctcctat tgagaaccag agtttcagcg agtttgagcg cggccatcat
     6121 tgaagaaggg tccgccttcc cctgatatgc gatatcatag gccgttccat gatccggaga
     6181 agttcggata tagggaagac caagggtcat attcacgcca tcatgaaaat gaatcatttt
     6241 gaaaggccct aacgcctgat catgatacat ggtgatcaaa gcatcaaacg cgccgcggcg
     6301 ggcagaataa aaaatctggt cggaagggta gggccctgac gcaagtattc cttttctttg
     6361 ggcgcgtctg atcgcgggac taataacagt aatttcctcc ccgccgccac actctttccc
     6421 gtgcggatta aaggcacaga ccgcgatttt gggatttttt attttaaagt catttttaag
     6481 cgcgtccgcc gtcaaaataa ttttttcgaa aatgaggtct gaggaaagaa ggccgctcac
     6541 tcttttaagc ggaacatgaa tggtcgcaag ggtcaatttc agactggcgc cgacgaacat
     6601 catcgcataa tgtttcacgc cagcggcttg agcgaaaaat tccgtgtgtc ccacaaatcc
     6661 cttttgggct aatcgggccg aaaccttgtt gatcggagcg gtcacaatcg cctggacact
     6721 cccctccttc gcaaggtcag aggcaattga aatagcgcgg attgccattt gaccattggc
     6781 gacggtttct cgcgatttat cgaacttcgc gcccccaatg tcccaaaaat aaacaatccc
     6841 cttctgcaaa acacgaccat cggaaggaaa tctttggaaa gaggaatcaa gactcgtctg
     6901 tttattaaga aaggaaaaaa ccgcctggga gccaatcact acatatcggc atcgggggcc
     6961 acgcttgagc cgtttcaggc acttcacaac aacttccggg ccaatcccgc ccggatcccc
     7021 catcgtgatg gcaataaccg gtgcccggac cgcagttgcc ggatgacgca gaagctgatg
     7081 tttcgtcatc cccgtacctc ccatccggtt tcagcgtatt gagatatagg ccgcccgctt
     7141 caactgttcc gtccattctt tgaagcgctc attacttttg gcccgaaaaa gatacccgga
     7201 aatctgctcc ctcgattcct caaatgtcac ctggcgggca tcttcttttt cttcaattcg
     7261 gaaaatgtga aatccaatgg gggattcgac aatatccgtc aactgtccct ggggcgtaga
     7321 aaagatcgct tcatccaccg attcgatcat cgaccctttc tcgatccagt ccgccggttt
     7381 ttcttgccgg gccctggaat cctcagaata tttcctgacc agatcgtcaa aattatctcc
     7441 ctgcttgatc tttaaataaa acgacttgat tttgtctctg gccgcctcat ccatcgtccc
     7501 ttgttcccgg gattcggcgc ttttcctgac cgtaagggac ttgatcttga cgcgagcagt
     7561 ccgctgaaac ttttcagggt tttctttgta aaagctttca atctccgcgg gcgaaataac
     7621 aacgcgggaa cgaatttgaa tatcctgaag tctccggatc atcagttgtt ttttgatttt
     7681 ctcccggaga gctttcatcg tcatcccctc tctctccaac atactgttaa tttcatcgta
     7741 cccttccatt ctcttgccga ggtcttccag ttcgcgatca atctcatctt ccgtaatcac
     7801 gatctcctgc gacaccgcct cctgataaac gagatgatct tcaatcaatt gatttaaaat
     7861 cttctgccgc gcctctttta caaaatcgac aagctcatcc ccgctgtatt gttctttgta
     7921 gtcttcgtaa aggggccgca aaatcgagtc cagctcggat tgggtaataa tctcgtcatt
     7981 caccaccgcg atcacccgat caagaagctc ttcctgggca ccacccagcg ggactccgag
     8041 ggaagcaaac aaacatagga taagcagaaa ctttttcata gtacgctcat tatacaacag
     8101 tttagaagcc gttcctagta agcgaaaaat tcatttttta agttactttc acttaagaag
     8161 actgagtttc tggttccggg ttgccggatc ggtgttcttc caccgctttt ttaagcattc
     8221 tgcagtaaag atcgaacccg acagcatgaa taaatccgct ttgttcatgt ccgagcaaat
     8281 tcccggctcc ccgtatttca agatcttcca tagcaatttt aaatcccgag ccaagctcaa
     8341 taaatcgctc aatcgcggca agccgttttg ccgcgtctgg agtcattgag ccacggggtg
     8401 tcaggaaata agcgtaggcc tgtctcattt ccttaaatcg tcccaccctg ccacgaagct
     8461 ggtataaatc cgccagtccg aagcagtccg cgcgattgac gatcaaagtg ttcgcattgg
     8521 ggatatcaat ccccgcctcg atgatgttgg tcgaaattaa acagtggatt gctcccttca
     8581 taaattgttt catcaccttt tcaagcattt caaccggcat ctgcccatgg gcgattccaa
     8641 attgaatatt cggtaaaatt tttctgagtt tttcatgcac tttttcgatg ttctcaacac
     8701 ggttatgcac aaaataaacc tgcccccctc gctctacttc ccgttcgatc gcctgccgaa
     8761 tcactaaatc gtgggattcc gttacgaagg tcttgatggg aagcctgttc cggggagggg
     8821 tgtcgatgac cgacatatca cgaacaccga gaagcgccat gtgaagcgtc ctcggaatag
     8881 gggttgcggt aagcgtcaat acgtcaatct gcgaacgcat ttttttgaga gattccttgt
     8941 ggcgaactcc aaatctttgc tcttcatcaa tgaccacaag tcctagttct ttaaatttaa
     9001 tgtccccgga cagaagtcgg tgggtaccca ccacaatatc agtcgcgcct gttttgagag
     9061 agcgaaccac ggccttctgt tccgaagggc tttgaaacct cgaaagaatc tccacggtca
     9121 cagggaacgt tttgacccga tttttaagta aaatgtagtg ctgttccgca agaatggtgg
     9181 tcggaacaag aaacgcaacc tgttttcccc ccgtgactgc cttgaacgcg gagcgaatcg
     9241 cgacctcagt ttttccgtat cccacatctc cacaaaggag ccgatccatc ggctttgagg
     9301 attccatatc acgtttcacc tcgtcgatag cccgcttctg gccttcggtt tcatcaaacg
     9361 ggaattcgtc ttcaaagatt ttttgccact catgatcccg gggaaacgcg aacccttgct
     9421 gcacattgcg acgcgcctgg agttcaagaa gatcgcccgc cactttgtgc agggcattgc
     9481 gagtacgctg tttgatccgc tcccattctt tggtgttcag ccgggtgagt ttaggggcgg
     9541 ccgaggaccc gccgatatag cgctcgatcg gctctttcat ggaaagataa aggatctcgc
     9601 cctcagcgta ttcaatcgca aaacatcgtt ctttttttcc ttccaaccgg atcattttgc
     9661 ttccgagaaa ccttccgatc ccgtagtgga agtgcacaac aaaatcacct cggtgaagtt
     9721 cgcccattcc ggtgacaggc tcaaaagctc caaacttttc ttccagtctt cgcaccttcc
     9781 gctcatagtg tcccgaaagt gggatcaaaa acacttcctc aaaaagggta aaactttttc
     9841 cttccggtgg agaaaagtcg cgaatttggt agagggtgtt tccgcggaag tcaagacgca
     9901 caggcatccg gtaagtcacc ggataaacat caaccgttcc tccccggata gaatattctc
     9961 cgggaagctc cacaagattt tttcgaacat agcgaagctc ctggagagtt gattccaggt
    10021 cctcaaaccg gattgtttca cctgtcttaa aatgtttaat gttcattttc taatggggac
    10081 ttgcttttta aaaccgttac cgccagcgcg attccatcaa aaaacttttt cgattaatgt
    10141 ctttcgtcaa aatctcaaga cgcccgatta aacctgatat tcatttttga ttcacaaaaa
    10201 gcaacaccga tgtatcgcgt ctcgggccaa ataactgggc gttttcacaa aagggattat
    10261 agaacagcgc tcaaagagcg gcacatgttt ttcatatttt caattgtcac gaaaattgaa
    10321 ctctatgagg tgttttatgg taagacgatt tttcgaggga aattaaaaaa acacacgctg
    10381 gcaggcaacc catccagaca gcgacttgag taaagccgcc gtttttgatc cgttcctcat
    10441 ccacaagcca atctatccgg ctctaaaaaa ctgctctttt cagaaacgag acttactttt
    10501 tttgcttaaa acagttagcc atgcgctcta ccgcggaata aatctctttc tcggaaacgg
    10561 cataacttaa ccggatgtgc tcagcgctac caaaaggttc tccgggaatc acggcaacgt
    10621 gagcttcttc aagaagtcgt ttggaaactt caaacgcgga aagacccgtc tttttgatgt
    10681 tcacgaaaag gtagaaggcc ccgccgggtt ttgtcgtcgt aaaacagtca atttttccca
    10741 ggagcgacag gaaaaggtcg cgccg
//
LOCUS       DAOSIO010000036        10678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_6078_length_10678_cov_4.595689, whole genome shotgun sequence.
ACCESSION   DAOSIO010000036 DAOSIO010000000
VERSION     DAOSIO010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 10678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10678
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6078_length_10678_cov_4.595689"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="PK590_02415"
     CDS             complement(<1..975)
                     /locus_tag="PK590_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HOG23484.1"
                     /translation="MKSYRITLIPGDGVGPELAETTRACLEATGVKFDWDIQTAGLDV
                     MESEGTPLPERVVESIRRNKVAIKAPITTPVGKGFRSVNVALRKELDLYACLRPCKSY
                     DGVRSRYKDIDLVIVRENTEDLYAGIEFQKGTAEALQLLKEIDRLSARKIREDSGISI
                     KPISVAGTERIVKFAFDYARQNKRKKVTAVHKANIMKFSDGLFLEVSREVAKQYPDIE
                     FEDRIVDNMCMQLVQKPEAYDVLVLPNLYGDILSDLCAGLVGGLGVAPGANIGTSGAL
                     FEATHGSAPKYKGLNKVNPTAMILSGVLMLKHLGETQAAERLEKAVSDV"
     gene            1177..1968
                     /locus_tag="PK590_02420"
     CDS             1177..1968
                     /locus_tag="PK590_02420"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOG23485.1"
                     /translation="MKTSKIKSTLLEAIETAGKIVKRAFGKKQRITQKGEFNIVTETD
                     KAAEKAALGVILKNFPHHSILAEESPEVRGSDGCWIIDPIDGTTNFAHGFPLTSVSIA
                     FEREGRLEMGGVLDPIRDEFFFAERGKGAFLNKKRIRVSEIKTLKDALVVTGFPYDRK
                     KNPDDYIAMLRAFMTRVQGIRRTGSAAIDLCYVASGRFDGYYEMKLNPWDKAAGMLIV
                     KEAGGHLTDFSGRPLSLTNVQNLATNGHIHAAMLSVLRSFKNLGK"
     gene            complement(2023..2355)
                     /locus_tag="PK590_02425"
     CDS             complement(2023..2355)
                     /locus_tag="PK590_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23486.1"
                     /translation="MKKIISLALVFSMVLVPAAFAASPWASGQNYGEKTGGKLGYGLT
                     NTVLGWTKIFSTPNDYSSAGKNVWQGVGQGLIDAGVTTVLGAVHLVTFPIPVDIDIPS
                     PVDLSGGK"
     gene            complement(2373..2534)
                     /locus_tag="PK590_02430"
     CDS             complement(2373..2534)
                     /locus_tag="PK590_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23487.1"
                     /translation="MSFREDNNFSAGKRSKKRLKKIAMACRYGIILLSEGIGCGKIRP
                     MVTLSEQGI"
     gene            2594..3670
                     /gene="mutY"
                     /locus_tag="PK590_02435"
     CDS             2594..3670
                     /gene="mutY"
                     /locus_tag="PK590_02435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOG23488.1"
                     /translation="MSFKPVISKKTFQKKLLSWFHKEKRPLPWRNNKDPYRIWISEIM
                     LQQTQVATVIPYYSRWLKAFPSLSSLARAPLSQVLTLWEGLGYYRRARMLHQTARFIA
                     RKLQGRIPEKADLLEQLPGIGRYTAGAIASIAFHEKVPVLDGNVIRVLTRLFAIRSSI
                     DKGRTLKTLWELAGSLLPDHSPGDFNQAMMELGATICHPQNPACGKCPLAKLCVSRRL
                     GNPMTFPRKTRKEKYEKIKMFTLILESENQKTFLCRQPPGHWWHGLWTFPFWDSRKEL
                     SDFLKQISCEAERVGKIRHSVTRYHITLDVYHSKLIRKIRPRVPWTGKWVRFEDLRRV
                     ALPSPHRKAATLAIRCAGKNRLLS"
     gene            complement(3733..3951)
                     /locus_tag="PK590_02440"
     CDS             complement(3733..3951)
                     /locus_tag="PK590_02440"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG23489.1"
                     /translation="MEQTKAQEKPQYPRRERSQIMTPKEAAKYLGFHLVTIYRLLKKG
                     EVPATKIGGQWRFKKDVLDSWLMDRMNQ"
     gene            4245..4832
                     /locus_tag="PK590_02445"
     CDS             4245..4832
                     /locus_tag="PK590_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23490.1"
                     /translation="MKIAKILASFVLCLALIVPSGFASDIWEKASSDTYKEKVGPMFV
                     RGLLNVVTSPVDILVQTVDKTKDGPPLIGTLTGMAGGLGCTALRAGSGLVDVGLFWVP
                     GFNGFPVNRSYHNCLLCDQVKSSDYYQAPVVQQVYTPQPVVTQTYTPQPVATQYVSEA
                     APQPVVSVVKEMPRAVEEVPAPAKKARDPYRYVKK"
     gene            complement(4904..5449)
                     /gene="pyrE"
                     /locus_tag="PK590_02450"
     CDS             complement(4904..5449)
                     /gene="pyrE"
                     /locus_tag="PK590_02450"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOG23491.1"
                     /translation="MEEQTLKEKLLKIIKEKAVIHGERKLASGKVSHYYIDGKQVTLD
                     PQGLFLTGKIILQVAQAVNADAIGGPTLGADPIAAAVSLLSSHSGQPVKAFIVRKEAK
                     DHGMQKMIEGPQIEEGDRIVIVEDVITTGGSVLKAIQEVEKLGGKVVKVTCLVDRNEG
                     AQETLASYNYSPIFTLKELGL"
     gene            complement(5520..6257)
                     /locus_tag="PK590_02455"
     CDS             complement(5520..6257)
                     /locus_tag="PK590_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOG23492.1"
                     /translation="MKVRNIIVILVCPENPDNIGAVARAIKNMGFESLRLVTPPARWR
                     FKGRKMAMSAGDILEKARVFRSLKDASADVSVLIGTTRRCGKERGAFLAFDRAIGKIR
                     RESLKGGVGIVFGCESKGLSNEHIRLCDYRMTIPTGRAYPSLNLAQAVMVILFSVFGK
                     RNLSNRESRAEEKPLKKKEVESTLMCFAGALRSLGYREGGANLLPRILQTTRDLIKRS
                     GLWEREAQMIKGLSRRITEKVDEFKRD"
     gene            complement(6277..6651)
                     /locus_tag="PK590_02460"
     CDS             complement(6277..6651)
                     /locus_tag="PK590_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23493.1"
                     /translation="MKKTMMLVILTGIIFCRPGLASETSTGPDPDLRQGNNVKVVMAR
                     GWKNFFTFPGEIVRTGQNEKQHHPKAWPLTYAPCLVSNVIIRATSSLNDMFVLPTYVW
                     ATKDPRPITHFFDLPDYVWEKV"
     gene            complement(6756..8048)
                     /locus_tag="PK590_02465"
     CDS             complement(6756..8048)
                     /locus_tag="PK590_02465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOG23494.1"
                     /translation="MQAQEDSRIIGARLRTARSLWSMAKKATMPADRFLSRFFFQNRK
                     KIGSRDRKFLSETIYNAFRHKTFLELWLREVLPRQNRVERDEDELFCILAAAKLGFVS
                     ELLFEELGLGEGVYSSLRGHVLPPNFVSSSPEETLAAVYSFPLWLVTRWTGRYGVEPT
                     ERLLKFFLSRPLLSIRVNPLKTSREKLIALFQKSGIASEPAKRSPYGLLIPERNNVPE
                     MPAFREGHFEVQDEGSQLVCQKTEAKPGEFIWDVCAGGGGKSLLLAAMMENRGRIVAT
                     DIRAHKLDELKKRAKRAGAFNIFPTDLNRVNELTVTKKGFDKILVDAPCSGTGTLARN
                     PDAKWRMDESWLQKFPAEQTEIIEKALPYLKKNGKLYYATCSLEPAENERVVEKILET
                     HPEMKCLPCGVSGENFFRLWPPESKTDGFFMAIMEKTQ"
     gene            complement(8260..8691)
                     /locus_tag="PK590_02470"
     CDS             complement(8260..8691)
                     /locus_tag="PK590_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23495.1"
                     /translation="MKKTLACVMILFLATMFVTPSAFAKGAKEFGASLLLPPSGQVMN
                     DEFGTTKSKIMMGIEVAAITTTIILGFVPGGAIVWAGVGPLLANHLWSATDAYVSAKR
                     KGQDPYLEGQMMEAQRTLDVSRQNRFARESDIRQRILSAGE"
     gene            complement(8815..10335)
                     /locus_tag="PK590_02475"
     CDS             complement(8815..10335)
                     /locus_tag="PK590_02475"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOG23496.1"
                     /translation="MKTVLVSKQGNAKTDILFVGVFKDEKKPGAIKKTEPLFARILEA
                     AAKKGRFSGKSQEIFASFQTSYRRASEVVAIGLGDKKDLKAVTLRKIAGKIIEIASAR
                     KALKIRVMADTFLGGALEARDVAAIFTELAGLATYRFFRYKTKKKDELPKFPETMELV
                     FDDQKTLKELKPVVSRAEMITKSVAYARDLNNEPGNVMNSSRLVEESRSLAKEKGLKC
                     TVLGLPELKKLGMNGLLAVNQGSVTPPALIILEHGEEHKAKGTVCLVGKGVTFDTGGI
                     SIKPSKGMEEMKFDKSGAIAVIATLGLLADLKLPLHVVGLAPCVENNVANDPQRPGDI
                     IRMYNGKTVEVVNTDAEGRLILADALSYCEKFKPVAIIDAATLTGLCHYTFGDKACAI
                     LGTDQKLIDVIREAGEKTGERCWQLPLWDDYADAIKGNHSDINNTGDGYAGTITATMF
                     LKEFVPAKVPWIHLDIAGTAYTAKNRYDCPKGATGFGVRLFAELLSNWNRENYLSA"
     gene            complement(10353..10634)
                     /locus_tag="PK590_02480"
     CDS             complement(10353..10634)
                     /locus_tag="PK590_02480"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOG23497.1"
                     /translation="MIYLPLMVMLKGMDTHRAVGTSLFVIVFTGAFGAFFHHKAGMVD
                     VKMALLMGIFSIAGVWVGTKISVNADPAILRKIFSVFLVVMAIKLFFTK"
BASE COUNT         2583 a         2981 c         2419 g         2695 t
ORIGIN      
        1 cacgtccgac acggcctttt caagacgctc cgcggcctga gtttctccca gatgtttaag
       61 catcaaaact cccgacaaaa tcatggcggt agggttgacc ttgttaagcc ccttgtactt
      121 gggagccgat ccgtgcgtgg cctcaaaaag cgcgccgctg gttccaatat tggcgcccgg
      181 cgccacgccc aatccgccca caagccccgc gcagagatcg gaaagaatat ccccgtaaag
      241 gttaggaagc accaaaacgt cgtacgcttc cggcttttgg acaagttgca tgcacatatt
      301 gtcgacgatc ctgtcttcaa attcgatatc cggatactgt ttggccacct cacgggaaac
      361 ctcgagaaaa agtccgtccg aaaatttcat aatgttggcc ttgtggaccg cggtcacctt
      421 ttttcttttg ttttgcctgg cataatcaaa cgcgaacttc acgatccgct ccgtcccagc
      481 cacggaaatc ggcttgatgc tgatccccga atcctcccgt atcttgcgag cggaaagtct
      541 gtcgatttcc ttgagaagct ggagcgcttc cgccgtaccc ttctggaact caattccggc
      601 gtaaaggtct tcggtgtttt cgcgcacaat cacgagatca atgtccttgt agcgggaccg
      661 aaccccgtca taggatttgc acggcctcaa acaggcgtaa agatccagct cctttctgag
      721 cgcgacgttc acggaccgga accccttgcc caccggagtc gtgatcgggg ccttgatcgc
      781 gaccttgttt ctgcgaatgg attcaaccac ccgttccggc aaaggcgttc cttccgattc
      841 catcacatcc aggcccgccg tctgaatatc ccagtcaaac ttcactcccg ttgcttcaag
      901 acacgcccgg gtcgtttcgg ccagctcggg acccactcca tctccgggga tcagggtaat
      961 acggtaagat ttcatacatc actcctctta taggtacggc tcagcttagt tgcgattcat
     1021 aagccgttcg gttacaatgc ccttccttct gaacggcata agctttttga cttttgttat
     1081 ttaaaatcct tggtacggaa agacaccttc cgccaaaact ttcttatgcc cgcttgatcc
     1141 atttaggaaa acacgctgaa aggaactgta ttttatatga agacctccaa aattaaaagc
     1201 accctcttgg aagccattga gacagccgga aaaatcgtga agcgggcctt cgggaaaaaa
     1261 cagcgtatca cccaaaaagg cgagtttaat atcgtcacgg aaactgacaa agccgccgaa
     1321 aaggccgctc tcggtgtcat cctgaaaaac ttcccccacc attccatcct cgcggaagaa
     1381 tctcccgaag tgcgtggttc ggatggttgc tggatcattg accccattga tggcacgacc
     1441 aatttcgcgc atggttttcc gctcacctcc gtatcgatcg ctttcgagcg ggagggccgt
     1501 ctggaaatgg gcggggtgct ggacccgatc cgggatgagt tctttttcgc tgaacgggga
     1561 aaaggggcat ttctcaacaa aaaaaggatc cgggtttccg aaataaaaac cctaaaggat
     1621 gccctcgtag tcaccggctt cccctatgac cggaaaaaaa atccggacga ctacatcgcc
     1681 atgctccgcg cctttatgac aagggtccag gggatccgcc gcacgggatc ggcggccatc
     1741 gatctttgct acgtggcgtc gggacggttt gacggttatt acgagatgaa actgaacccc
     1801 tgggacaagg ccgccggaat gctgatcgta aaggaagccg gcggacattt aacggatttt
     1861 tcaggccgtc cgctttccct gacgaacgtt caaaacctcg ccaccaatgg tcacatccac
     1921 gccgcgatgt taagcgttct tcgctccttt aaaaacctcg ggaaataaaa aacgccccgt
     1981 ccggagacgg ggcgtttcaa aaaataactt ctgatcttct tcttacttcc cgccgctaag
     2041 atccacggga ctggggatat caatatccac cgggatcggg aaggtcacca aatgaacggc
     2101 gccaagaacc gtcgtcacgc cggcatcaat taacccttgt cccactccct gccagacatt
     2161 ttttcccgcc gaagaataat cattcggggt agaaaaaatc ttcgtccaac cgagaactgt
     2221 gttagtcaga ccatagccaa gtttaccacc ggttttttct ccgtagttct gtccgctagc
     2281 ccagggactc gccgcgaacg ccgcaggaac gagaaccatg gaaaacacca acgccaagct
     2341 gattattttc ttcactgttt ttcctccttg gtttaaatgc cctgttctga aagagtaacc
     2401 atcggtcgta ttttgccaca accaatccct tccgacaaca ggataatgcc gtatcgacac
     2461 gccatcgcaa tcttctttaa tctttttttt gatcgttttc cggcagaaaa attattgtct
     2521 tcacggaagc tcatttcgtg aaagtttttt caggggcttg tcgccaaact tcgcgcggga
     2581 tatgataccc ggcatgtctt tcaagcctgt catctccaaa aaaacttttc agaaaaaact
     2641 gctttcctgg ttccataaag aaaaacgtcc gcttccctgg cgaaacaaca aggaccctta
     2701 caggatttgg atttcggaaa tcatgctcca gcaaacccag gtggcaaccg tgatccccta
     2761 ttactcccgg tggcttaaag cattcccctc gctgtcatca ctggcccgag ctccgctcag
     2821 tcaggtcctc accctatggg aaggattggg ttattatcgg cgggccagaa tgttgcatca
     2881 aaccgcccgg ttcatcgccc ggaagttgca gggcaggata ccggaaaaag ccgacttgct
     2941 tgaacaactg cccggcattg gccgctatac ggcgggagcc attgccagta tcgcttttca
     3001 cgaaaaggtc cccgtcctgg atggaaatgt catccgggtt ttgacccgac ttttcgcgat
     3061 ccgttcgagc atcgacaaag gtcgaacctt aaaaaccctg tgggagcttg ccgggtcact
     3121 tttaccggac cactcacccg gagatttcaa tcaggcgatg atggagctgg gcgccaccat
     3181 ttgccatccg caaaatccgg cctgcgggaa atgccccctg gcgaaattat gcgtctcacg
     3241 ccgccttgga aatcccatga cctttccccg taaaacccgg aaagaaaaat atgaaaaaat
     3301 aaagatgttt acgctaatcc tggaatctga aaaccaaaaa actttccttt gcagacaacc
     3361 tccgggccac tggtggcatg gactatggac ttttccgttc tgggactccc gaaaagaatt
     3421 gtccgatttc ctgaagcaaa tttcatgcga ggccgagagg gtgggaaaaa tccggcacag
     3481 tgtaacccgt tatcacatta ctttggatgt ttaccattca aagttaattc gaaaaatacg
     3541 cccgcgtgtc ccctggaccg gaaaatgggt gcggtttgaa gacctccgga gagttgctct
     3601 gccgtcccct cacagaaaag ccgcaactct cgcaatcaga tgcgcgggga aaaacaggct
     3661 actttcttga catctcagac aataccaccg aaaaaacatc cgtttgtccg ggtatcgaaa
     3721 cccgcgacgc tcttattggt tcattcgatc cattaaccag ctatccagaa cgtccttctt
     3781 gaaacgccat tgtccgccaa tttttgttgc aggaacttct cccttcttga ggaggcgata
     3841 aatcgtcaca aggtgaaacc cgagatactt cgccgcttcc ttgggcgtca ttatctggct
     3901 tctctcgcgc ctcgggtact gaggtttttc ctgcgctttg gtttgttcca ttatttttct
     3961 ccttattgag ttgaatttgc tatttttaag actgatatag ttatatacta caatacaaac
     4021 tacaccaaat actataaatt gtcaatagat gctataaatt acaacttctt tatatttttg
     4081 taacttattg atatgtttaa tgttacaaaa aaatgtcccc gccaaaaaaa aatcctgttg
     4141 attgttattt ttattaggtg tactattccg gttgcgatca atttatagta atcttctgct
     4201 accgtcgcta tcattaaaag aaaatttcaa aagggaggtt gctcatgaaa attgcgaaga
     4261 ttttagcatc gtttgtactc tgccttgcgc tcatcgttcc ctcaggattc gccagtgaca
     4321 tctgggaaaa agcgagttcg gacacttaca aggaaaaggt cggtcccatg tttgttcgtg
     4381 gccttttgaa cgttgtcacc agtcccgttg acatccttgt ccagaccgtc gataaaacca
     4441 aggacgggcc tccacttatc ggaacgctca cagggatggc cgggggtctc ggttgcacag
     4501 ctctccgggc aggttccggg cttgtggatg tcggcttgtt ttgggttccg ggttttaacg
     4561 gatttccagt caaccgatcc tatcacaatt gtttgttgtg cgatcaagtg aaatcttcgg
     4621 attactatca agccccggtg gttcagcagg tttatactcc tcagcccgtg gtgacgcaga
     4681 cgtatacgcc tcagccagtc gcgactcaat acgtctccga ggcggccccg caaccggtcg
     4741 tttcggtagt taaagaaatg ccccgggcgg ttgaggaagt tcccgcgccc gcgaaaaaag
     4801 cgcgtgatcc ctaccggtac gtgaagaaat aagcattctc caaaaacaaa aaacccgttt
     4861 acgtttcaaa cgtaaacggg tttttttatg ccgatagtcc gaattacaaa cctaattctt
     4921 tcaaagtaaa aatgggggag tagttatagc tcgctaaagt ttcctgagcc ccttcgttcc
     4981 ggtcaaccag acatgtgact ttcactactt tcccgccaag tttctcaacc tcctgtattg
     5041 ccttgaggac cgaccctccg gtcgtgatca cgtcctccac tatcacaatc ctgtctccct
     5101 cctcaatttg ggggccttct atcattttct gcataccgtg atccttggct tctttgcgaa
     5161 cgataaaagc cttgaccggc tgcccgctat gagagctaag aagggaaaca gccgcggcaa
     5221 tcggatcggc gcccagagtc ggccccccga tcgcgtccgc gttgacggcc tgcgccacct
     5281 ggagaatgat cttccctgtc aagaacaaac cctgtggatc aagagtcact tgcttgccat
     5341 caatgtaata atgcgacacc ttcccggaag caagtttccg ctctccgtga atcacggctt
     5401 tttccttgat aattttgagc agtttttctt tcaacgtctg ttcctccatg atgtggctcc
     5461 ttcacaaaaa acctccgagc cgaggcgtcc gtaaatccca attcggggag agtatatcat
     5521 taatcccgtt tgaattcatc gaccttttcg gtaatcctgc gggaaagccc cttgatcatt
     5581 tgagcctctc tctcccaaag cccgcttcgc ttgatgagat ctctcgtcgt ctgcaagatt
     5641 ctgggcaaaa gattggcgcc tccttcccga taaccaagcg accggagggc ccccgcaaaa
     5701 cacatcaagg tcgattcgac ttcctttttt ttgaggggtt tctcttcggc cctggattcc
     5761 cggttcgaca aattcctttt cccaaaaact gaaaagagaa tcaccatgac agcttgcgca
     5821 agattcaggg acggataggc gcggcccgtc ggaatagtca tccgataatc gcataaacga
     5881 atatgctcgt tggaaagacc tttggattca cagccgaaaa caatcccgac gccacccttc
     5941 agcgattccc tccggatttt tccgatcgcc ctgtcaaacg caagaaacgc gcctcgctcc
     6001 ttaccgcatc gccgtgtcgt tccaatcaaa acgctgacgt cggcggatgc gtcttttaaa
     6061 gagcgaaaaa ctctcgcctt ttccaagata tcgccggcgg acatcgccat ttttcttccc
     6121 ttgaaccgcc atcgtgccgg gggcgtcaca agacggagtg attcaaatcc catattcttg
     6181 atcgcccggg ccacggcacc gatattgtct gggttttcgg gacagacgag aatgacaatg
     6241 atattccgaa ctttcatgaa aggggtctag aggcgttcag actttttccc agacataatc
     6301 gggcaaatca aaaaaatggg taattggccg cgggtctttc gtcgcccaaa cataggtcgg
     6361 aagaacaaac atgtcattca gagaagacgt ggccctgatg atcacgttcg acacaagaca
     6421 gggcgcgtat gtgaggggcc aggccttcgg atgatgctgt ttttcattct gtccggtcct
     6481 cacgatttct ccgggaaatg tgaaaaaatt cttccaccct cttgccatca cgaccttaac
     6541 gttgtttccc tgccggaggt cagggtcagg cccggtggat gtttccgaag ccaggcccgg
     6601 ccgacaaaaa atgattcccg tcaagatcac caacatcatt gtttttttca tggtctcccc
     6661 tttgcccttt tggcatattg aactgattaa atcacttttc ccccgataaa tccgaaaacc
     6721 gctttacaac tagaggtttt ctctgcctct tctctttact gcgtcttttc catgatcgcc
     6781 atgaaaaaac catcggtttt gctttcgggc ggccacagcc tgaaaaagtt ctctcctgaa
     6841 acaccacacg gcagacactt catttcagga tgcgtctcaa ggattttttc aaccactcgc
     6901 tcgttttccg ccggttccag agagcaggtc gcgtagtata gtttgccgtt tttcttcaag
     6961 tatggcaaag ctttttcaat aatctccgtt tgttcagcgg gaaatttttg aagccacgat
     7021 tcgtccattc tccatttcgc gtccgggttt cgcgccaatg tcccggtccc cgaacaggga
     7081 gcgtccacca aaattttgtc gaaccctttt tttgtgaccg tcaattcgtt cacgcgattc
     7141 aaatcggtcg ggaaaatgtt aaacgccccg gcccgtttgg ccctcttttt taattcatca
     7201 agtttatggg cccggatgtc cgtcgcgaca attcttcccc tgttttccat catcgcggcc
     7261 agcaaaagac tcttccctcc ccctcccgcg caaacatccc agataaattc tcccggtttc
     7321 gcctctgtct tttgacaaac aagctggctc ccctcatcct ggacctcaaa gtgtccttcc
     7381 ctgaaggcgg gcatttcagg cacgttgttc cgttccggaa tcagaaggcc ataaggggat
     7441 cgcttcgccg gttctgacgc aatgcccgat ttttgaaata gcgcgatcag tttttctcgg
     7501 cttgttttaa ggggattgac tcgtatggaa agtaaagggc gggagagaaa aaacttcagc
     7561 aatctttcgg ttggctccac cccgtaacgc cccgtccaac gcgtcacaag ccagagcgga
     7621 aacgagtaaa ccgcggcgag tgtttcttcc ggagaacttg acacaaaatt cggcggaaga
     7681 acatgccccc gcagggaaga ataaacgccc tccccaagac caagctcctc gaaaagtaat
     7741 tcgctcacaa aacccagttt agccgcggca aggatacaaa aaagctcatc ctcgtcccgt
     7801 tcgacccggt tttgccgggg caagacctcc cgaagccaga gttcgagaaa tgttttatgc
     7861 cggaaagcgt tataaatggt ctcggagaga aatttgcggt cacgggatcc gatctttttc
     7921 cggttttgaa aaaaaaagcg actcagaaac cggtccgctg gcattgtcgc tttttttgcc
     7981 atactccaga gagatcttgc cgtccggagc cgagccccga taatcctgct atcttcttgc
     8041 gcctgcatag ggattatttt ccccgtccta tcaataattt tttaacctgg tcaagacaac
     8101 gggagttttt cccgttccag ctccagagac accttcctca cggagcttcg gacccacaaa
     8161 ccaaaaaggc ctgatccgtc caaagcaacg aatgaacaac ccttcgtcgg gacgatcagg
     8221 cctttcagga gatctacggg tctgactaaa aactttttct tattcgcccg cgctcaaaat
     8281 gcgctgacgg atatcggact ctctggcaaa ccggttttgt ctggaaacat ccagggtcct
     8341 ctgcgcttcc atcatctgcc cttccagata ggggtcctgg cccttccgtt tcgccgacac
     8401 ataggcgtcc gtcgcggacc aaaggtggtt ggcaagtagc ggcccaacac cggcccaaac
     8461 aatggcgcct ccggggacaa agcccaggat aatggtcgtg gtgatcgcgg cgacctcaat
     8521 ccccatcata atcttggact tagtggttcc gaactcatcg ttcatgacct gtccggaggg
     8581 aggcagtaaa agcgacgccc cgaattcttt ggctcccttg gcaaacgccg aaggggtcac
     8641 aaacatggtg gctagaaata atatcatcac acaagcaagg gtcttcttca tggtcagtct
     8701 ccttaaatta aaacacgctc cagcctccaa gttaacggct tgaaggggaa aaaactttaa
     8761 acttccccca ctcaaaaaca aaccggtttt gaaggttggc gtttttttta tagattacgc
     8821 gctgagatag ttttcgcggt tccagtttga aagaagctcg gcaaaaagtc tgaccccaaa
     8881 tcctgtcgct cccttgggac aatcgtaacg gtttttggcc gtataagccg ttcccgcgat
     8941 atcaagatgg atccacggaa ctttggccgg aacaaactct ttgagaaaca tcgtcgccgt
     9001 aatcgtcccc gcgtaaccat caccggtatt gttaatgtcg gaatgattcc ccttgatcgc
     9061 gtccgcgtaa tcatcccaaa gaggaagttg ccagcaacgc tctccggttt tttctccggc
     9121 ttcgcgtatc acatcaatca gcttctggtc cgttccaaga attgcgcacg ccttgtcacc
     9181 gaacgtgtaa tggcaaagtc ccgtaagcgt cgccgcgtca atgatggcta ccggcttgaa
     9241 cttctcgcaa tacgaaagcg cgtccgcgag gatgagccgg ccttcggcgt ccgtgttgac
     9301 cacctccacg gttttcccgt tatacatcct gatgatgtca ccgggccgtt gaggatcatt
     9361 cgccacatta ttttccacgc atggcgcaag cccgaccacg tgaagcggca acttcaggtc
     9421 cgcaagaagt cccagggtcg cgataacggc aatggcgccc gatttatcaa acttcatttc
     9481 ttccatccct ttagacggct tgatcgagat cccgcccgta tcgaacgtca cgccttttcc
     9541 gaccagacag accgttccct tcgctttatg ctcttcccca tgttccagaa taatcagcgc
     9601 cggaggggtc acgcttccct ggttaaccgc cagaagaccg ttcatcccga gctttttcag
     9661 ttcgggaaga ccgagaaccg tacactttaa tcccttttct ttcgcgaggc ttctggattc
     9721 ctcaaccagc cgggaagagt tcatgacgtt gcccggctcg ttgttaagat cacgggcgta
     9781 agcgacgctc ttcgtgatca tttcagcccg gctgacaacc ggctttagct ctttcaaagt
     9841 tttctgatcg tcaaaaacca gctccatggt ttccggaaac ttcggaagct cgtctttttt
     9901 cttcgtcttg taacggaaaa atcggtatgt cgcgagacct gcaagttccg taaaaatcgc
     9961 ggcaacatct cgcgcttcca gagcgccgcc caaaaaagtg tccgccatca cgcggatctt
    10021 caaggctttt cgcgcgctcg cgatctcaat gattttcccg gcaatcttgc ggagggtgac
    10081 cgccttcaaa tcctttttat caccgaggcc gatcgcgacg acttccgaag cgcgccgata
    10141 agacgtctgg aatgaagcga aaatttcctg ggattttccg gaaaatcgcc ccttcttcgc
    10201 ggcagcctcc aggatcctgg cgaacaaggg ttctgttttt tttatggccc caggcttttt
    10261 ttcatcttta aaaacgccca caaaaaggat gtccgttttg gcgttacctt gtttggaaac
    10321 gagaactgtt ttcattgaaa acctccggaa agttatttgg taaagaaaag tttaatggcc
    10381 atgaccacaa gaaacactga aaaaattttt ctaagaattg ccgggtccgc attcaccgaa
    10441 attttcgtgc ccacccacac gcccgcaatg ctgaagatcc ccatcaaaag cgccatcttc
    10501 acgtcaacca tgccggcttt atgatggaaa aaagcgccga aagctcccgt aaaaacaatc
    10561 acaaaaagcg acgtcccgac cgcccggtgg gtgtccattc ctttcagcat caccataaga
    10621 ggcagataaa tcacgccgcc gccaagcctc gactgcggta ttgagttctg cgttcaca
//
LOCUS       DAOSIO010000037        10564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_6158_length_10564_cov_4.525169, whole genome shotgun sequence.
ACCESSION   DAOSIO010000037 DAOSIO010000000
VERSION     DAOSIO010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 10564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10564
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6158_length_10564_cov_4.525169"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /gene="sufB"
                     /locus_tag="PK590_02485"
     CDS             <1..1230
                     /gene="sufB"
                     /locus_tag="PK590_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOG23498.1"
                     /translation="HYPPIDYQNIIYYSAPKSEKEGPKSLEDVDQEILKTYEKLGIPI
                     EEQKRLAGVAVDAVFDSVSVATTFKEKLRESGVIFCPFSEAVREHPELIEKYLGSVVP
                     VRDNFFSALNSAVFSDGSFCYIPKGVRCPMELSTYFRINTAETGQFERTLIVAEDDSY
                     VSYLEGCTAPIRDKNQLHAAVVELVAHDRASIKYSTVQNWYAGDREGKGGIYNFVTKR
                     GLCKGVRSKISWTQVESGSSITWKYPGCVLMGDDSVGEFYSVAIVNNHQQADTGTKMI
                     HIGKRTKSTIISKGISARHANATYRGLVKILPKAVQARNFTQCDSLLIGHQCGANTFP
                     YIEVENHSSKAEHEATTSKIDEDQLFYCRQRGMSPDNAVSMIVNGFCKEVFKNLPMEF
                     AVEASRLLGVTLEGSVG"
     gene            1376..2146
                     /gene="sufC"
                     /locus_tag="PK590_02490"
     CDS             1376..2146
                     /gene="sufC"
                     /locus_tag="PK590_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015005861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOG23499.1"
                     /translation="MLQIKNLHVSIEGKEILKGIDLAIQAGEVHAVMGPNGSGKSTLT
                     RVLAGHPSYQIMKGTVTYEGKDLLRMSVEDRARAGVFLAFQYPIDIPGVNNAYFLRSA
                     LNEIRKSRGLEELDAMDFLTVLKEKMKIVEMDEKFISRPVNEGFSGGEKKRNEILQMA
                     VLEPNLAVLDETDSGLDIDALRVIAGGVNKMRNRSRAILLVTHYQRLLNYIEPDKVHV
                     LMGGKIVLSGGKDLALQLEEKGYEWVSPVRDPSSAFDA"
     gene            2180..3481
                     /gene="sufD"
                     /locus_tag="PK590_02495"
     CDS             2180..3481
                     /gene="sufD"
                     /locus_tag="PK590_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOG23500.1"
                     /translation="MSNMKELQPYEEAYQRLGNQRQAEAEWHRVMRDDAMRSFLGKGF
                     PTTRDESWKYTDLSAIAGARFIMEPTTSRVVFDGTEVPKNSMDAYFMFFANGWFRAEL
                     SDLPEGIRGMILSEIRSGDRFRTILDELNVFTKDSLEDLNFALGMDGIFIDVPPGVCL
                     EKPIYIVHRSDCHEGLCQPKTIMRLGKNAQAVLIEDFRGVDGKMCFTNSFAGVVLEEG
                     ASLEHYKLQHESRGSFHIATFKAVQKEKSVLNSISFALGAKLARQTAMIALNGQRSEC
                     NLSGLVLGAEHQTLDHVTRVVHAVPDCKSDQRFKGILGGHSRGVFTGRVLVQRDAQKT
                     DARQTNRNLLLSETARADSRPQLEILADDVKCTHGATVGQLDEDAIFYLRSRGIGQKT
                     ARRILASGFAQEIVDSIRHEKFRIFINDMTQKRFLDLEATA"
     gene            3478..4701
                     /locus_tag="PK590_02500"
     CDS             3478..4701
                     /locus_tag="PK590_02500"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOG23501.1"
                     /translation="MSLLDIQKIRDDFPLLREKVYDKPMIYLDNAATTQKPRAVIDTV
                     ERFYAKENANIHRGIFCLSEQTTRRYEGARYKVREFLGADSEKEIVFVRGATEAINLV
                     AQTYGRAFLKAGDEILITHMEHHSNIVPWQMLRDAIGVKLKVAPVNDSGELIMEEFRN
                     LLTDQVKFVSVAHVSNALGTINPVKEIIRLAHEKGAKVLVDGAQAVAHRVVNVRDLDT
                     DFYVLSGHKLFAPTGIGVLYAKSELLESMPPYQGGGDMIRSVTFEETTYNRIPYKFEA
                     GTPHIAGVIGLGAAIDYLREVGLDTIEAYEHELLEYGARRLLEIPGLRLIGTARNKSA
                     ILSFVLGEAHAHDIGTILDRAGIAIRAGHHCAMPLMQRFGVPATARASLAFYNTKEEI
                     DCLTNALREVTEVFK"
     gene            4698..5153
                     /locus_tag="PK590_02505"
     CDS             4698..5153
                     /locus_tag="PK590_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HOG23502.1"
                     /translation="MNEEIRELYQQMIMDHNKRPRNFRKLSAATAQAEGFNPLCGDHL
                     HVYVKMDGNRIEDISFEGSGCAISRAAGSMMTDALKGKTREEAKAIFEEVRAMLSRDV
                     HQPFEKKHLGKLEVFAGICEFPARIKCASLPWHAMMEALEPTGKQVTTE"
     gene            5219..5542
                     /locus_tag="PK590_02510"
     CDS             5219..5542
                     /locus_tag="PK590_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HOG23503.1"
                     /translation="MAEKTLEEKIWDELRLCFDYEIPVNIVDLGLVYSVRLNDLDGGG
                     KEAVIEMTFTSAMCPMADTIKRDVERRVGSVEGIKKVTLQIVWDPPWDVSRMSEAAKL
                     QLGML"
     gene            5677..6054
                     /locus_tag="PK590_02515"
     CDS             5677..6054
                     /locus_tag="PK590_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23504.1"
                     /translation="MDKKEFYAKSEKIINDTFKAVKKYAKIMAKKTGEAASVTKLLIR
                     RATLEHQIAKACSRLGSKVYDQTVRKGETINLEDAEVVSVIDEIKKLEQQHEDVEITL
                     EKERSKLKSSKTKSGKGKTKSST"
     gene            6093..6177
                     /locus_tag="PK590_02520"
     tRNA            6093..6177
                     /locus_tag="PK590_02520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6127..6129,aa:Leu,seq:gag)
     gene            6247..7617
                     /locus_tag="PK590_02525"
     CDS             6247..7617
                     /locus_tag="PK590_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014955834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOG23505.1"
                     /translation="MIPSHIFREYDIRGKANAELTEEVALAIGKAFGTVLVREGKKKV
                     AVGHDHRWSAEFLYRGLTEGLRSTGLTVIRIGLVPTPVVYFYVTTQNMDAGISLTGSH
                     NPPDENGFKFHHATRSFYGTDIQNLRNLIERNDFVTGDGKVETPDHESLLKDYENYIA
                     NIFKFTKRLKVVIDTGHGMGAVTALGLVKRFGHDLTVLYGDLNSKMPDHQADPSVPAN
                     MKDLQAKVLELKADIGIAFDGDADRIGVVNEKGEIIYGDKLLLIYARELLARKPGATI
                     IGDVKSSKQVYDGIAKAGGKPLMWKTGHSLIKAKMKETGAAMAGEMSGHMFFKDRWLG
                     FDDAVYAACRVLEILDRANAPFSSLLSGVPNLPSTPEIRFDCPEAIKFRVVEKAIENF
                     KRQYTVVDIDGARVEFADGWGLVRASNTQPALVMRFEAQTEKRLQEIRALIEGEVKKI
                     YQEMNG"
     gene            7614..8666
                     /locus_tag="PK590_02530"
     CDS             7614..8666
                     /locus_tag="PK590_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOG23506.1"
                     /translation="MNWAIIMAGGSGTRFWPESRKSRPKQFLSLFGRQTLLEQTFHRL
                     RRFIPRDRILVFVSESKVALTRQLLKIPAGQVIGEPVGRNTAPCALWAASYVMRKYPS
                     AVLGIFPADHYIRDEKAFTKAMRLSYGIAGQKGMPVTLGICPDFPHTGYGYLEMGRRL
                     APGVFYLKQFREKPDLPSAKKYLKSKRFLWNAGIFIWRADCLLEAGRQYLPDVFKITT
                     AIAGEGCSRTSVKKLFSKCPAISIDYGLMEKMTGQILTVPVSMGWNDVGGWASLKKLL
                     ARRSNDNVSQGKTLLVESSGNFVRSQTKLIATVGLQDHVVIETSDAILVCPMKKTEEI
                     RKIVSALETAGMGQFL"
     gene            8753..9613
                     /locus_tag="PK590_02535"
     CDS             8753..9613
                     /locus_tag="PK590_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOG23507.1"
                     /translation="MNSVSDLTLWKAVLLGLVQGLTEFLPISSSGHLVLFQNIFGMKD
                     PMISFDIALHAGTLIAVMVYFWGEIMETLTGFYGFLQGKKEMGCCEKNLDGRATHVSP
                     LLWIYILITLIPTGLMAVGFRKGIESAFADLRIVAVCWLVMGCALVLSGKFGKGTRLM
                     SEMGWRRALGIGAAQAVALFPGISRSGSTILAGMATGLKSDEAAKFSFLIYIPAIAGA
                     IVLDLKDGLTYFRYCGWETLIGFLSAAVSGYFVIRWLMAIIKKARFFIFGYYCVALGG
                     ILLFHLLMSR"
     gene            9620..9928
                     /locus_tag="PK590_02540"
     CDS             9620..9928
                     /locus_tag="PK590_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23508.1"
                     /translation="MNLLRVITRNGKDKSVWNRHLIPEINVPAIKKLSPEGLLSDFSR
                     LAYISGDLFQKVVSCVAAAAERNVRILQKTGQPREFPVNIYYFEEGYLKGLFYEVIWP
                     "
     gene            9925..10452
                     /locus_tag="PK590_02545"
     CDS             9925..10452
                     /locus_tag="PK590_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOG23509.1"
                     /translation="MMEIYQGIKFEVVRVPDESVADTRVDELILAGKTLSTKGFCPAN
                     SGNMSFRRPDGFVITRAGSELGKLEPRDFVWVKDVDIGQKKVFAVGTANPSSEAMMHF
                     LIYGARPEVSVILHAHALDLKNAVITKESHPYGTLEFAHSAVEILKNHDLVILKGHGF
                     VSVGGTVEESLSKIR"
BASE COUNT         2613 a         2327 c         2998 g         2626 t
ORIGIN      
        1 cactaccctc caattgatta tcagaatatt atttattatt ccgcgccgaa atccgaaaag
       61 gaaggaccga aaagtctgga ggatgtcgat caggaaattt tgaagactta tgaaaaactt
      121 gggatcccga ttgaggagca aaaacgtctc gcgggggtcg cggtggacgc ggtctttgat
      181 agtgtgtccg tggccacgac tttcaaggaa aaactcaggg agtcgggagt gattttttgt
      241 ccgttttccg aggcagtccg ggagcatccc gagctgatcg aaaagtatct cggaagcgtg
      301 gttccggttc gggacaattt tttttcggcg ctgaattccg cggttttttc ggacgggtcc
      361 ttttgttaca ttcccaaggg ggttcgttgc ccgatggagc tttcgaccta tttcagaatt
      421 aacacggccg aaacggggca atttgagcga acgctgatcg tggcggagga tgactcctac
      481 gtcagttatc tggaaggctg tacggccccc attcgtgaca aaaatcagct tcacgcggct
      541 gtcgtggagc tggtggccca cgaccgggct tcgatcaagt attcgacggt ccagaattgg
      601 tacgcgggag accgggaagg gaaaggcggc atttataatt ttgtcacgaa gcgtggtctt
      661 tgcaaggggg tgcggtccaa gatttcatgg acgcaggttg agagcggttc ttcgattacc
      721 tggaaatatc cgggttgcgt tttgatgggg gacgattcgg tcggggagtt ttattccgtc
      781 gcgatcgtca acaatcatca acaggcggat accggcacca agatgataca catcggaaaa
      841 aggacgaaga gcacgattat ttccaaggga atttcggcgc gacatgccaa cgcgacttat
      901 cgtgggcttg taaaaatttt acccaaagcg gtccaggcca ggaatttcac gcaatgcgat
      961 tctcttttga ttggtcatca atgcggcgcg aatacgttcc cgtacattga agttgaaaac
     1021 cactcatcga aggcggaaca cgaggccaca acttccaaga tcgatgaaga ccagcttttt
     1081 tattgccgtc agcgcggaat gtcacccgac aatgctgttt ccatgatcgt gaacggtttc
     1141 tgtaaggaag tgttcaaaaa cctccccatg gaatttgccg tggaggcatc acggttgctc
     1201 ggggttaccc tcgagggaag cgttggctaa ttcgcgggtt tttaaagatg aatcccctga
     1261 tgagttttcg ataaaaacta agggataatt gggatcgacc cgcctccgca gtttgtttct
     1321 tgatcggagt tcaaaagaaa cagcagggtg tggaaaatta attaaaggag ccgtcatgct
     1381 acaaatcaaa aacttacacg tcagtatcga aggcaaagaa atccttaagg gaatcgatct
     1441 ggcgatccag gcgggagaag tgcacgcggt gatgggcccc aacggatccg gaaagagtac
     1501 gctgacccgg gtactggccg gacatccgtc ttaccagatt atgaagggga cggtgacgta
     1561 tgagggaaaa gatcttcttc ggatgtccgt ggaggatcgc gcccgtgccg gtgttttttt
     1621 ggcctttcag tatcccatcg atattcccgg agtgaacaat gcctattttc tgcggtccgc
     1681 ccttaacgag attcgaaagt cccgcgggct ggaggaactg gatgccatgg attttcttac
     1741 cgtgctgaag gaaaaaatga agattgttga aatggatgaa aaatttatct cccgtcccgt
     1801 gaacgagggt ttctcgggcg gagaaaaaaa acggaatgaa atactgcaaa tggcggtcct
     1861 tgaacccaat cttgcggtgc tggatgaaac ggactccgga ctggatattg acgcgcttcg
     1921 ggttatcgcc gggggcgtca ataaaatgcg gaaccggagc cgggcgattc ttcttgttac
     1981 gcattatcag cgtcttttga actacattga acccgacaag gtgcatgtcc tgatgggagg
     2041 caagattgtt ctttcagggg gaaaagatct ggcgttacag ctcgaagaaa aagggtacga
     2101 atgggtgagc cccgttcgag atccgtctag tgcgtttgac gcttagtcca acctgtttta
     2161 aatcggataa agggttttca tgtccaatat gaaggaatta caaccgtacg aagaagccta
     2221 ccagagatta gggaatcagc gtcaggcaga ggcggaatgg caccgggtga tgcgagacga
     2281 tgccatgcgg tcatttctgg gaaaaggatt ccccacgacc cgggatgaat cctggaagta
     2341 cacggactta agcgcgattg ccggcgcgag gtttatcatg gaaccgacaa cctcccgggt
     2401 ggtgtttgac gggacagagg ttccgaagaa ctcgatggat gcctatttta tgtttttcgc
     2461 caacggctgg tttcgcgcgg agctttccga tcttcctgag gggatccggg gcatgatact
     2521 ttcagagatt cgatccggag atcggtttcg gacgatcctg gacgagttaa acgttttcac
     2581 gaaagattcg cttgaagatc tgaattttgc tttggggatg gacgggattt ttattgatgt
     2641 ccctccggga gtttgcctgg aaaagccgat ttatattgtc caccggtcgg attgtcatga
     2701 aggtctttgc cagccgaaaa cgatcatgag attggggaaa aatgcccagg ctgttttaat
     2761 cgaggatttc cggggcgtgg acgggaagat gtgttttacg aattcgtttg ccggagtggt
     2821 tctcgaggag ggcgcgtcat tggagcatta caagttacag catgaaagcc ggggctcttt
     2881 tcacatcgcg actttcaagg cggtccaaaa agaaaaaagc gtcttgaatt ccatttcctt
     2941 cgcgttgggt gccaaactcg cgcgacagac cgcgatgatc gccctgaatg gacagcgatc
     3001 agagtgcaac ctgtccggac tggttttggg agcggaacat cagacgctgg atcacgtaac
     3061 gcgagtggtc cacgcggttc cggattgtaa aagcgatcag agattcaagg ggattttggg
     3121 cggtcattca cgcggtgttt ttaccggccg ggtgcttgtg caacgggatg cccagaaaac
     3181 ggacgctcgc cagacgaaca gaaatctgct tctttctgaa acggccaggg ccgactctcg
     3241 tccccaactt gaaattttgg cggatgatgt caaatgtacc cacggcgcta ccgtcggcca
     3301 gcttgacgag gatgccattt tttatcttcg ttctcgcggt ataggccaga aaaccgcgcg
     3361 gagaattctc gcttcggggt tcgcgcaaga aattgttgat tcgattcgtc atgaaaaatt
     3421 taggattttc atcaatgaca tgacccaaaa gagatttttg gatctggagg caaccgcatg
     3481 agtttgctcg acatacagaa aatacgcgac gactttccgc tgttgcgcga aaaggtctat
     3541 gataagccga tgatttatct tgataacgcg gcgacgacgc aaaagcccag ggctgtgatc
     3601 gataccgtgg agcggtttta cgcgaaggaa aacgccaata ttcatcgcgg catcttctgt
     3661 ttaagcgaac agacgacccg gcgttatgaa ggcgcacgtt acaaggtccg ggaatttctg
     3721 ggggccgatt ccgaaaaaga aattgtgttt gtgcgtggag cgacggaagc gatcaatctg
     3781 gtcgctcaaa cctacgggcg tgcctttttg aaagcgggcg atgaaattct gatcacgcac
     3841 atggaacacc actccaacat tgttccgtgg caaatgcttc gggatgcgat cggagtgaag
     3901 ctcaaagtgg ctccggtgaa tgattcgggt gaacttatca tggaggaatt cagaaatctt
     3961 ttgacggatc aggttaaatt tgtgtccgtc gctcatgtgt caaacgcgct tgggacgatc
     4021 aacccggtca aagaaatcat tcggctggca cacgaaaaag gcgccaaagt tctggtcgat
     4081 ggggctcagg cggtggcgca ccgggtagtg aatgtccggg accttgatac ggatttttat
     4141 gtgctttccg gccacaagct tttcgcgcca acgggaattg gcgtgcttta cgcaaaatcg
     4201 gaattgctcg aatccatgcc gccttatcag ggtggagggg atatgatccg gtccgtgact
     4261 tttgaggaaa ctacttacaa ccggatccct tacaaatttg aggcggggac accgcatatc
     4321 gcgggtgtga ttggccttgg cgccgcgatt gactatcttc gggaagttgg gctcgacacg
     4381 atcgaggcgt atgagcacga attgctggag tatggagccc ggagactttt ggaaattccg
     4441 ggtctcaggt taatcggaac ggcccggaat aagtcagcga tcctttcgtt tgttctgggc
     4501 gaagctcacg ctcacgatat cgggacaatt ctggatcgcg ctggtatcgc gattcgggca
     4561 gggcatcatt gcgccatgcc gcttatgcag aggtttggcg tcccggcgac ggcccgcgcc
     4621 tcgcttgctt tttataacac aaaggaagaa atcgactgtt tgacgaatgc cctgagagaa
     4681 gtcaccgagg tgtttaaatg aatgaagaga tacgggaact ttatcagcag atgatcatgg
     4741 atcataataa acggccaaga aatttccgga aactttcggc cgcgacggct caggcggaag
     4801 gatttaatcc tctttgcggt gatcaccttc atgtttacgt taaaatggac ggcaaccgga
     4861 ttgaggacat cagctttgag ggttcaggtt gcgcgatttc gagggcggca ggttccatga
     4921 tgacggacgc gttgaaggga aaaacccgtg aggaggccaa ggccattttt gaagaggtcc
     4981 gcgccatgct ttctcgcgac gttcatcaac catttgaaaa aaaacatctg gggaagctgg
     5041 aagtctttgc ggggatttgt gaatttcccg cccgcattaa atgcgcgtcg cttccctggc
     5101 atgccatgat ggaagcgctt gagccgacgg gaaaacaggt gacgaccgaa taattcaacg
     5161 acacaagacc gaaactcctc gtgaggcgcg tggcgaggag cgtgtagatg gaaaagtgat
     5221 ggctgaaaag actctggaag aaaaaatctg ggatgagctc cggctttgct ttgactatga
     5281 gatccccgtt aacatcgttg atcttggtct cgtttactcc gtaagattga acgaccttga
     5341 cgggggcggc aaggaagcgg tgattgagat gactttcacg agcgcgatgt gcccgatggc
     5401 cgacaccatt aaaagagacg ttgaaaggcg cgtcggatcg gtcgaaggta ttaaaaaagt
     5461 caccctccag attgtttggg atccgccctg ggacgtatcc cggatgtctg aagcggccaa
     5521 gctccagctc gggatgttgt agaaatcggg cgccgaaaat tgaggttcaa ttccatgttg
     5581 atatcttttt taaatatgat aaaacatact gcccttcggg ggttttttcg gcgttgaggc
     5641 atttcttcca aacctaaaag aaaagcggga gtttccatgg acaagaagga gttctacgca
     5701 aagtctgaaa aaattattaa tgacactttt aaagcagtga aaaagtacgc gaaaatcatg
     5761 gccaaaaaaa cgggcgaggc ggccagcgtc accaaactcc tgattcgaag ggcaaccctg
     5821 gagcatcaaa tcgccaaagc ttgttccaga cttggaagca aggtttacga tcaaaccgtg
     5881 cgcaaggggg aaacgattaa tcttgaggac gctgaggttg tgtcggtgat cgatgagatt
     5941 aaaaagctgg aacagcaaca cgaagacgtt gaaatcactt tggaaaagga acgaagcaaa
     6001 cttaaatcct cgaaaaccaa aagtggcaaa gggaagacga aaagtagtac ttaggatcgt
     6061 tcgtactgct gtcaatccca ggctaagttt aggcgcgggt ggtgaaattg gcagacacgc
     6121 cagcttgagg ggctggttcc gcaaggagtg caggttcaag tcctgtcccg cgcaccattt
     6181 acaacaaccc atgcgtcaga ggttcaaggt ttaagctgga caccctggct tcctggtagg
     6241 agaaacatga taccatccca tatttttcgt gaatatgata ttcgtggcaa ggcgaatgcg
     6301 gagttaaccg aagaggtcgc tcttgcgatc ggcaaggcct ttgggactgt gctggttcgg
     6361 gaagggaaga aaaaagtcgc ggttgggcac gatcaccgct ggagcgcgga attcctttat
     6421 cggggtctga ccgaggggct tcggagtacc ggactcaccg tgatccggat agggcttgtc
     6481 ccgaccccgg tcgtttattt ttatgtcacg acccaaaata tggacgcggg catttccctc
     6541 acgggatctc acaacccgcc ggatgaaaac ggtttcaaat ttcatcacgc gactcgatct
     6601 ttttacggca cggacattca aaatcttcgt aacctgattg agcggaatga ttttgttacg
     6661 ggagacggaa aggtcgaaac gcctgatcat gagtcactgc tcaaggatta cgaaaattat
     6721 atcgcgaaca ttttcaagtt tacgaagcgg ttgaaagtgg tgattgatac ggggcacgga
     6781 atgggagccg tcaccgcgtt ggggctggtg aagcggttcg ggcatgacct gacggttctt
     6841 tacggggatt tgaattctaa aatgccggat caccaggcgg atccttcggt gccggcgaac
     6901 atgaaggatc ttcaggcgaa ggtgcttgaa cttaaggcgg atattggaat cgcgtttgac
     6961 ggggatgccg accggattgg tgtcgtcaat gaaaaagggg agatcatcta tggcgataaa
     7021 ctcctcttga tctatgcccg agaacttttg gcacgaaaac ccggcgcgac tattatcggg
     7081 gatgtcaaga gctccaaaca ggtatacgac ggtatcgcga aagccggtgg caaaccgctg
     7141 atgtggaaaa cgggccattc cctgatcaag gcgaaaatga aagagacagg cgccgccatg
     7201 gcgggagaga tgagcgggca tatgtttttc aaggatcgct ggcttggatt tgacgatgcg
     7261 gtttacgcgg cgtgccgggt tcttgaaatt ctggatcggg ccaacgcgcc tttttcgtcc
     7321 cttctttcgg gcgtgccgaa ccttccgtcg acccctgaaa ttcgctttga ttgtcccgag
     7381 gcgattaaat tcagggtggt tgaaaaagcg attgaaaatt tcaagcggca gtacacagtg
     7441 gtggatatcg acggcgcccg cgttgaattc gcggacggtt gggggcttgt gcgggcttcc
     7501 aacactcagc ccgcgctggt gatgcgattt gaagcccaga cggaaaagcg gctccaggaa
     7561 attcgcgcgt tgattgaagg tgaagtaaaa aagatttatc aagagatgaa cggatgaact
     7621 gggcgatcat catggcgggg ggaagtggca cccgcttttg gcccgaaagt cgcaagagtc
     7681 gtcccaaaca gtttctctcg ttatttggca ggcagaccct ccttgagcag acattccatc
     7741 gccttcgaag atttattccc cgcgaccgta tcctggtctt cgtgtctgag agtaaggtcg
     7801 ctctgacgcg gcaacttctc aaaatcccgg cgggacaggt tatcggagaa ccggtgggac
     7861 ggaacacggc gccctgcgcc ctctgggcgg cttcttatgt catgcgaaaa tacccatcgg
     7921 ccgttttggg gatttttccc gccgatcatt atatccgtga cgaaaaagcg tttacgaaag
     7981 caatgcgcct gtcttacggg attgctggac agaaagggat gccggtgacg ctcggcattt
     8041 gtccggattt tcctcacacg ggttacgggt atctcgaaat gggacggcga ctggctcccg
     8101 gagtttttta tctgaagcaa ttcagagaga agccggatct tccttccgcg aaaaaatatc
     8161 tgaaatcgaa acgttttctc tggaatgccg gaatttttat ctggcgcgcg gactgcttgc
     8221 tggaagctgg ccgtcaatat ttgccggatg ttttcaagat cacgactgca attgccgggg
     8281 aagggtgttc ccgaacatcc gttaaaaaac ttttttcgaa gtgtcccgcc atatcaattg
     8341 attacggtct gatggagaaa atgacggggc aaatacttac cgtgccggtt tcgatggggt
     8401 ggaatgacgt ggggggctgg gcttcactca aaaaacttct cgcgagaaga tcgaatgata
     8461 atgtcagcca ggggaaaaca cttctcgtgg aatcttccgg aaacttcgtg agatctcaaa
     8521 ctaaactgat tgctacggtt ggactgcaag atcatgtcgt tattgaaacg tctgatgcca
     8581 ttctggtttg cccgatgaaa aagactgaag agatacgaaa aattgtttcg gcgcttgaaa
     8641 ccgccgggat ggggcagttc ctttgacggt ttcggagagc ttccgtttta acgcgggcgg
     8701 ggaggtttag ttttatgttc cttcagtttt ttgcgacctg gagaaaaacg ccgtgaattc
     8761 tgtttctgat cttaccttgt ggaaggccgt cctccttggc ttggtccagg gattgacgga
     8821 atttctgcct atctcgagca gtggtcacct cgtattattt caaaatatat ttgggatgaa
     8881 agaccccatg atctcctttg acatcgcgct tcacgcgggg acgttaatcg cggtcatggt
     8941 ttatttctgg ggtgagatta tggagaccct gacgggattt tatggtttcc ttcagggtaa
     9001 aaaggaaatg ggttgttgtg aaaaaaatct cgacggtcgc gcgactcatg tatctccgct
     9061 actttggatc tatattttaa tcacgctgat ccccaccggg ctgatggcgg ttgggttcag
     9121 gaaggggatc gagtcagctt ttgccgatct ccggattgtg gcagtttgct ggctggtgat
     9181 gggatgcgcg ctcgtcttga gcggaaaatt cgggaagggg acccggctga tgtccgagat
     9241 gggatggcga agggccctgg ggattggcgc cgcgcaggcc gtggcattat tccccggtat
     9301 ttcaagatcg ggaagtacta tcctggcggg aatggcaacg gggctcaaaa gcgatgaggc
     9361 ggcgaaattt tcttttttga tttatattcc cgccatcgca ggcgccatcg ttctggatct
     9421 gaaggacggt ttaacttact tccgctattg cgggtgggaa accctgatag gttttctttc
     9481 ggcggctgtt tccggatatt ttgtcattcg ctggctgatg gcgattatca aaaaagcccg
     9541 tttttttatt tttggctact actgcgtcgc gcttggtgga attcttcttt tccatctttt
     9601 aatgtcgcgg taactcccca tgaacctctt gagggttatc acccgtaacg gcaaagataa
     9661 atcggtttgg aatcgccatc tcattccgga gatcaatgtg cccgcgataa aaaaactctc
     9721 accggaaggg ctactttctg atttttcccg cttggcgtat atctctggcg atctttttca
     9781 gaaagtcgtt tcttgtgtcg cggcggccgc ggaacgaaac gtccggattc tccaaaaaac
     9841 aggacagcct cgcgagtttc ccgtcaatat ttattatttt gaagaaggat atctgaaagg
     9901 tcttttttat gaggtgatct ggccatgatg gaaatttatc aagggatcaa atttgaggtg
     9961 gtgcgcgtgc cggatgaaag cgttgcggat accagagtcg atgagcttat cctcgcgggc
    10021 aagactttat cgacgaaagg attttgcccg gccaatagcg gcaatatgag cttccggagg
    10081 ccagacggat tcgtcatcac acgggcagga tccgaacttg gaaaattaga gcctcgcgat
    10141 tttgtttggg tgaaagatgt tgatattgga caaaaaaagg tttttgcggt gggcacggcg
    10201 aatcccagtt cggaggcgat gatgcatttt cttatttacg gcgctcggcc ggaagtatct
    10261 gtcatcctcc atgcccacgc ccttgatctg aagaacgcgg ttatcacaaa agagtcccat
    10321 ccgtatggga ccctggagtt tgctcattcg gccgttgaaa ttttaaaaaa tcacgatttg
    10381 gtcattttga aagggcatgg ttttgtaagc gtgggaggaa ccgttgaaga atctttgtcg
    10441 aaaatccggt gagtttttca tgggtatttt acgaaaactc cgaatagggg agacttttta
    10501 aaaaacatta gaaactatgt tttataaaaa aaatatcacc ttcctcttgg agaagagata
    10561 ggct
//
LOCUS       DAOSIO010000038        10263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_6358_length_10263_cov_4.456701, whole genome shotgun sequence.
ACCESSION   DAOSIO010000038 DAOSIO010000000
VERSION     DAOSIO010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 10263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10263
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6358_length_10263_cov_4.456701"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /gene="groEL"
                     /locus_tag="PK590_02550"
     CDS             <1..823
                     /gene="groEL"
                     /locus_tag="PK590_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011526708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOG23510.1"
                     /translation="LQIAAVKAPGFGDRRKAMMEDIAILTRGKFISEDLGIKLENVDL
                     DMLGTAKRVVIDKDNTTIVGSAGSEKEIKARCEQIKAQIEKSDSDYDKEKLQERLAKL
                     SGGVAVVKVGAATEVEMKETKARMEDALHATRAAIEEGIVPGGGVALLRSIASLDGLK
                     LQGDEETGAQIVYRALQEPARRIAYNAGFDGSVVVKKILEGKGSFGFNAQSGEFSDLI
                     EDKVIDPARVVRVAVENSTSIAGLILTTECAVAEKLKEKKENEKKPASNYAGSIQ"
     gene            complement(848..1543)
                     /locus_tag="PK590_02555"
     CDS             complement(848..1543)
                     /locus_tag="PK590_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG23511.1"
                     /translation="MAVRSGGGLSKLFLVMLLGVTVFAWWSDTYAVLIQVTLVAATVG
                     WVMKRILGQGVPRRGLQWFYGILFFPVLLFPLLRCHYKVPYVFCRACPSPCPWGISRT
                     FLFNGFLILNLLGRSWCSNLCPFGTFQELQSSIFRKHLRQPSWLNGVAYFILLATLGF
                     YLAPLFRWPWLNWFDVGNYDFVKVTAIAALLIMLTAFLIPKFWCRNLCPVGTLHLLFM
                     RLRIKKTGDKHFR"
     gene            2037..5150
                     /locus_tag="PK590_02560"
     CDS             2037..5150
                     /locus_tag="PK590_02560"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOG23512.1"
                     /translation="MTVESSRKNSSSLSRSPGEGIEIERYFTNAGENPLDKVIYDQRT
                     SVITNPDGSVVFKMDNVEVPEGWSQLATDIAVSKYFRKAGVPETGSETSVRQLIFRVA
                     HAIRLAGEKLGGYFKTSGDAQTFEDELIHLLVNQNCAFNSPVWFNCGLDLYNIKGSGG
                     NWYWDPSTDTLLETTDAYSHPQCSACFIQSVEDDLMSIFDLVKNEARLFKYGSGTGTN
                     FSKIRGKMERLSGGGTSSGLMSFLEVLDRGAGATKSGGTTRRAAKMVCLDMDHPEIVD
                     FINWKVREEKKVAALIAAGYSSDFNGEAYKTVGGQNANNSVRISNAFMKSYLEDGKWQ
                     TTFRTTGQVAETFETRHLMRQISFAAWACADPGVQFDTTINDWHTCANTDKIRASNPC
                     SEFMFLDNTACNLASINIMKYLNQDGSFDIEAFRRTIRIFTLAMEIIVDHASYPTQEI
                     AKNSHLYRPLGLGYANLGTFLMVSGIPYDNPRAFAVCSALTAIMTGHAYKTSAEIAAA
                     KGPFAEYQKNESSMLKVIEKHRAAAYQIPAEHCWDDLLKAAQEDWDLALEFGKHHGYR
                     NAQVSVIAPTGTIGLLMDCDTTGIEPDFALVKFKKLAGGGYFKIVNQSVPESLKRLGY
                     HPAEIQDIIEYIQGTARLEGTPWINRQTLGEKGLLAEDLEKIEAVLPSVFDLGFAFTK
                     WTLGEETMKRLGFKPEDYNHPGFNPLESLGFSRSEIEEANNVVCGMMTIEGAPHLKHE
                     HLPIFDCANKCGKYGKRFLEPMAHVRMMSAAQPFISGAISKTVNLPKETTVEEIEDIY
                     IQAWKMGVKAVALYRDGSKLSQPLNTKFKDSADEKSDLPKLQRRRLPKKRTGMTIEAR
                     VGGQKIYLRTGEYEDSALGEIFIDIHKEGAAFRSMMNCFAIAVSLGLQYGVPLDEFVN
                     VFTFTRFEPQGMVEHPNIKMSTSIVDYIFRVLGMEYLGRTDFVQVPPDPSTLAFARKR
                     EARPVKNDTVEINLKEPSRGKASGANEHLSRMMGDAPFCPNCGHVTVRSGACYKCLNC
                     GSSLGCS"
     gene            complement(5278..10218)
                     /locus_tag="PK590_02565"
     CDS             complement(5278..10218)
                     /locus_tag="PK590_02565"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23513.1"
                     /translation="MSGVVKGRFVKEPKAHGFIFLVSLFVVIAPSIANLGSAAVVGTW
                     PLWGRALVSIVTTMLSFAGLWGEIYLLTGILLDSGKHIAPSGDTIFKNVSTGMKKGLA
                     YSGILMTLLYAMAIVFMTHTAQQIMMSFPYLIGILAGILVFPLVKTIIESFDGSLPFF
                     DRLAFSYRNPVLYARGAVVGFGFAAMIVQGYIDKAMSDRVTFGLIIGLIASGGVSLFR
                     DIVYMTRGCGKIQTWRLYLVDSLLGGFLGSAVAFYLDAPQVSVVLYKFKLYVTTRFSQ
                     DVYHNYPYFNSLGHYVQPVLISKWGLTDLGFCPGGVKLLFTESLDGVIKWSVATWLFA
                     FNKVFMQAFFDKDKTPIKFFFSKAGVAQLSELMIYVLRWGLWMSPIIYTFLRMMPDPT
                     WYNQDGAIRTVFATWNSLTMSPGDFRAWSLTVFVWILAFDFFRILIWMDHMGLRVATL
                     VNLSFLGMDKLDEKIARFIGPAAAQRYIPEGVKRFTTWAPLLIPFYLPRGQEWDHVWS
                     TAEQMQNASRGKGLIPWIQSLSASQTLLLLVVSVAVMTGVVSIVRAFRGRSRKRQVAE
                     FSIANREYRVTMKENSEVYGEVLGKECDVSRRSYDLVDPCGRVLFLVDTAKEPESKDR
                     AWPVCGNFPERYFSPSKAEKTEDVLRITNSSHGIQTSVEITLPDQDSTAELWTVTLEN
                     RTNEARKLKIIPYLEWVLNGWMHDRFHTQYQRLYPEMQYVNASNAMLSWNKSTKSMGF
                     LASDIAPEGFLTGRVDFIGRARSVWAPRIVETLDFLEAKDTPACPTFDPIGSFLIHAP
                     LAAGEKKTVRLMIGYAKKKEQALEMVTKHLKPDPTKISSDFQPGKHSLLIGHGEIPEG
                     TPQPYYEFKNQGNTLAVKTPYTPRPFDHGMSNPVHSVMVTNRGLHTSCNGNSQQNRLT
                     PDWPDTVTREIPSEAIYLYEPESREWYSPTHHPLNDRAAKNECEFSVDGTAVFRMSKG
                     NLSTELTVFVPTGDPMGVYLLTVRNHENRPRKIRVAPYFQMALAFQPERSGPLVTKYD
                     KTLDALFFENPRNIFRSGPAFVSMSIPADCFETRRGRFFGSGRGPVDPYLVENAIPDE
                     TQLTDQAQIAAFVGTLDLPARGERTVAIILGQADRKGQAVQLVQKYKNMETVRKTLES
                     TKAWWLGMMGTVTLQTNQPEFDRYLNWLKYQAIAERIWARRGFYQTSGAFGFRDQLQD
                     TVNLTWVDPALARKQIILHASQQFIEGDVFHWFFTLTDGRSAFSCRSHASDNLLWIAW
                     GVGEYIRITGDDSILDEMTSYVSSEFPFAPLPKNKHGLGALYGRSTRMDTVYRHCMRS
                     IDTVLERKMGRNGLPLMGVGDWNDGLDEIGSGGKGESVWLGFFLYYILREMLPAIERK
                     DGIRRKEHYAAKMKSLAEALEKTWRGDRYLRAIHDDGTEIGVKDSGVWEIDALTAAWS
                     VMTGINFERGVTIFNTALSVLERGSVILLGWPALREDTKPYLGRSSKYPEGVRENGMY
                     CHGVQWLVRAARLITEEYEKRGDHAKADEYRATAFRLWSKISPLAHVTPEEIEIYGGQ
                     PNKQPADFLTTFEPGRMIWNGYTGAAGWMLRQAFEGVVGAILVHNEMILPEDLTKPRG
                     PIWVLKVDRDISKSPFRKEFDLTNPQRKVAIFPPES"
BASE COUNT         2689 a         2826 c         2505 g         2243 t
ORIGIN      
        1 gctccagatc gcggcggtca aagcgcccgg ttttggcgat cgccgcaagg ccatgatgga
       61 agacatcgcg atcctcaccc ggggcaaatt catctccgag gatcttggta taaaactcga
      121 gaacgtggac cttgatatgc tcgggaccgc gaaacgcgtg gtgatcgata aagataacac
      181 caccatcgtg gggagcgccg gctcggaaaa agaaatcaag gcccgctgtg agcagatcaa
      241 ggctcagatt gaaaaatccg actcggatta tgacaaagag aagctccagg aacggctcgc
      301 gaaactttca ggcggcgtgg cggtcgtgaa agtgggagcc gcgaccgagg tcgaaatgaa
      361 agaaaccaag gcccgcatgg aagacgcgct tcacgcgacc cgcgcggcca ttgaggaagg
      421 tatcgtcccc ggcggcggag ttgccctttt aaggtcgatc gcgtctttgg acggcctgaa
      481 gctccagggc gacgaagaga caggcgccca gatcgtgtat cgcgcgctcc aggaaccggc
      541 acgcagaatc gcttataacg ccggatttga cggttcggtc gttgtcaaaa aaatcctgga
      601 aggcaaaggg agttttggct ttaacgcgca gtccggtgaa ttctcggatc tgatcgaaga
      661 taaagtcatc gaccccgccc gggtggtccg ggttgcggtg gagaattcca cttcaatcgc
      721 ggggctcatc ctcacgactg aatgcgcggt cgcggaaaaa ttgaaggaaa agaaagaaaa
      781 cgaaaagaaa ccggcttcca actacgccgg cagtatccag taatacaaac cggggacagt
      841 cgctttttca cctaaagtgc ttgtccccgg ttttttttat cctcaaacgc atgaacagca
      901 agtgaagagt tcccaccggg caaaggttac ggcaccagaa cttcggtatc agaaacgcgg
      961 tcagcatgat cagcaaggcc gcgatcgccg ttactttcac aaagtcatag ttcccaacgt
     1021 caaaccagtt cagccaaggc catctgaaaa gcggcgccag ataaaaccct aaagtcgcga
     1081 gtaatatgaa ataagcgacc ccgttcaacc acgaaggttg ccgcaaatgt tttcggaaga
     1141 tcgagctctg cagttcctga aacgtcccaa aagggcaaag atttgaacac caggacctgc
     1201 ccagaaggtt gagaatcaaa aaaccgttaa acaaaaatgt ccgcgaaatc ccccacgggc
     1261 acggcgacgg acacgcgcgg caaaaaacat acggaacctt gtaatgacat cgtaaaagag
     1321 gaaacagaag caccggaaaa aacaagatcc cgtagaacca ttgcaatccc cttcgcggga
     1381 caccttggcc caaaatacgc ttcatcaccc aaccaaccgt cgccgcgacc agcgtcacct
     1441 gaatcaatac ggcataagta tcactccacc aggcaaaaac ggtcactccc aaaagcatta
     1501 caagaaaaag cttgctaagc cccccacccg acctgacggc catacctcct ccttcccaaa
     1561 ccgcgcgcct gaaaatattg tcgctccaga ataacatgat tttccctgtt ttaaaaatgg
     1621 acggttctat tttttaaaaa agtaaatttt tcattttgtc agttccgtta atttgacgcg
     1681 acaacaggac atttctgttt cgactggaaa aaatttctga atttctgttt gacttcactt
     1741 ttctctttgt tagaattcct cccgtttgcg tcacacgttt ttttaggaca atgatttttg
     1801 agctccaaca ccgtcgggac ttagctccgg atccgggacg gttgaagatg atggcacgga
     1861 gcgaaagact tgccactctt aatggataac gcgaaaacga gaaatctgaa agtgttcgca
     1921 ctaaatacta catatagtat ttatcccttc caaacaccac aaaatataga caaagtttat
     1981 aaaagttttt atctaacacc ctttaccgaa caactacctg accggagatc aaacccatga
     2041 ctgttgaatc atccaggaag aattcatcgt ctctctcacg ctcacccggg gagggaattg
     2101 aaatcgaaag atattttaca aatgccgggg aaaatcctct ggataaagta atctacgatc
     2161 agagaacatc cgtgatcaca aatcctgacg gctcggttgt ttttaaaatg gataacgtgg
     2221 aggtcccgga ggggtggagc caactggcca cggacatcgc ggtttcaaaa tatttccgca
     2281 aggcgggagt tcccgagacg ggcagcgaaa caagcgtccg ccagctcatc ttcagggtcg
     2341 ctcacgcgat ccgcctggcg ggagaaaaac ttggaggcta tttcaaaacg tccggggatg
     2401 cccaaacctt tgaagatgaa ttaattcatc ttctcgttaa ccaaaattgc gctttcaatt
     2461 cccccgtctg gtttaattgc ggccttgatc tgtacaacat taaagggagc ggcggtaact
     2521 ggtactggga cccgtccacc gatactctcc tcgagacaac cgacgcttat tcccacccac
     2581 agtgttccgc ctgttttatc cagtcggtcg aggatgattt gatgtcgatc ttcgaccttg
     2641 tcaaaaatga agcgcggctt ttcaagtacg gttcggggac cggcaccaat ttttccaaga
     2701 tccgcggaaa aatggaaagg ctctcgggcg gaggaacgtc ttctgggttg atgtcctttc
     2761 tcgaagtgct tgaccgaggc gcgggcgcta ccaaatccgg agggaccacg cgacgagccg
     2821 ccaaaatggt ttgcctggac atggaccatc ccgaaattgt ggattttatt aactggaagg
     2881 tacgcgaaga aaaaaaagtc gccgccctga tcgcggccgg gtattcctcc gattttaacg
     2941 gtgaagctta taaaaccgtc ggcgggcaaa acgccaacaa ctcggtccga atctctaacg
     3001 cgttcatgaa gtcttatctc gaagacggaa aatggcaaac cacctttcga accaccgggc
     3061 aggtcgctga aacatttgaa acgcgccacc tgatgagaca gatttctttc gcggcctggg
     3121 cctgcgcgga tccgggagtt cagtttgaca ctacgatcaa cgattggcat acatgcgcca
     3181 atacggacaa aatacgggcg tccaatcctt gctcggagtt tatgttcctc gacaatacgg
     3241 cctgcaacct ggcttcaatc aacatcatga agtatttgaa ccaggacggt tcctttgaca
     3301 ttgaggcttt tcgccgaacg atccgtatct tcacactggc catggaaatc atcgtcgatc
     3361 atgcctcata tccgacccag gaaatcgcta aaaacagtca tctttatcgc ccgctgggat
     3421 tgggttacgc gaatctggga acattcctca tggtcagcgg aattccctac gacaacccca
     3481 gggcctttgc ggtctgctca gccctgacgg caatcatgac cggacacgct tacaagacct
     3541 cggctgagat tgccgccgcc aaaggcccgt ttgccgaata tcagaaaaac gaatcatcca
     3601 tgctgaaggt gatcgaaaaa catcgcgccg ccgcttacca aatccccgcg gaacactgtt
     3661 gggatgatct cctcaaggcc gcccaggaag attgggatct tgccctggaa ttcggaaaac
     3721 accacggtta ccggaacgcc caggtctcgg taatcgctcc caccggaacg atcggtttgc
     3781 tcatggattg cgacaccacg ggtattgagc cggattttgc gcttgtcaaa ttcaagaaac
     3841 tcgcgggggg aggttatttc aagatcgtga accagtccgt tcccgagtcg cttaaaagac
     3901 tgggttatca ccccgcggag atccaggata tcattgaata tatccaggga acggctcgcc
     3961 ttgaagggac cccgtggatc aatcgccaga ccctcggtga aaaagggctc ctcgcggaag
     4021 atcttgaaaa aatcgaggcc gttcttccat ccgtatttga tctcggcttc gcgttcacaa
     4081 aatggacgct tggcgaggaa accatgaaac gtctcggatt caagcccgaa gattataatc
     4141 acccgggttt taacccgctc gagtcactcg gattttcgcg ttccgagatc gaagaagcca
     4201 acaatgtggt ctgcggcatg atgacgattg aaggagcgcc tcacttaaaa catgaacatc
     4261 ttcccatttt tgactgtgcc aacaaatgcg gaaaatacgg gaagcggttt ctcgagccaa
     4321 tggctcatgt caggatgatg tccgccgcgc aacccttcat ttccggcgcg atctccaaga
     4381 cggtgaatct gccgaaggag acaacggtcg aggaaattga ggacatttat atccaggcct
     4441 ggaaaatggg agtcaaggcc gtggcgcttt accgtgacgg ctcaaaactt tcacaacccc
     4501 tcaatacgaa attcaaagat tctgccgacg aaaaatccga cctccccaaa ctccagagac
     4561 ggagactccc caagaaacga actggcatga ccatcgaggc ccgggtcggt ggacaaaaaa
     4621 tttacctccg gacgggagaa tacgaggaca gcgccctcgg tgaaattttt atcgacatcc
     4681 ataaagaagg cgccgctttc cgctccatga tgaactgttt cgcgatcgcg gtatcgctcg
     4741 gacttcagta cggagttcct cttgacgagt tcgtcaacgt attcactttc acaagattcg
     4801 agccgcaagg aatggtcgaa catcccaata tcaaaatgtc cacctcgatc gtcgactaca
     4861 tttttcgggt cctcgggatg gaatatcttg gccgcacgga ctttgtccag gtcccgccag
     4921 acccctccac tctcgctttt gcccggaaac gggaggcccg acccgtgaaa aacgatactg
     4981 tggagataaa tctgaaagaa ccctcgagag gaaaagcttc cggggctaat gaacatcttt
     5041 cccggatgat gggcgacgcg cccttttgcc caaattgcgg gcacgtcaca gtgagaagcg
     5101 gcgcttgcta taaatgtctg aactgcggaa gcagtctggg ctgttcctga gcgaaatagg
     5161 agttagcttt tactttctcc ggaatttcgt cgctccccct catgggggca aactatcttt
     5221 cctgaaaaaa agcctctccc ccgaaaaaca gggaagaggc ttttttcgtc aaaaccctta
     5281 agactccgga gggaaaatag caacctttcg ttgcgggttt gttaaatcaa actccttccg
     5341 gaacggactt tttgaaatat cacgatccac cttaagcacc cagataggcc ctctcggctt
     5401 cgtgagatcc tccggcaaaa tcatttcatt atgcacgagg attgcgccca ccacaccttc
     5461 aaaagcctgg cgaagcatcc atcccgccgc tcccgtgtag ccattccaga tcatccggcc
     5521 cggctcaaac gtcgtaagga aatcggctgg ctgtttgttc ggctggccac cgtagatttc
     5581 aatttcctca ggcgtcacgt gggcaagcgg tgaaatcttt gaccagagac gaaatgcggt
     5641 cgcgcgatat tcgtcggcct tggcatgatc accccgtttt tcatattcct ccgtgataag
     5701 cctcgcggcg cgcaccagcc actggacacc atggcaatac atgccgtttt cacgcactcc
     5761 ttccggatat ttactgctcc ggccaagata aggttttgtg tcctcccgta aagccggcca
     5821 tccgagaaga atgacgcttc ccctttccag gacactcagc gctgtgttaa agatcgtaac
     5881 accacgctca aaattgatcc cggtcatcac cgaccaggcg gcggtcaagg cgtcaatttc
     5941 ccagaccccg ctatccttca cgccaatttc cgtgccatcg tcgtggatcg cccgaaggta
     6001 ccggtcgcca cgccaggtct tctcaagagc ttcggcaagc gatttcatct tcgcggcgta
     6061 atgctccttc cgccggatac catctttgcg ttcaatggca ggtagcatct ctcgcaaaat
     6121 gtagtagagg aaaaaaccga gccacacact ctcacctttt cctccactcc cgatttcatc
     6181 caaaccgtcg ttccagtctc ccactcccat caggggaaga ccattccggc ccatcttgcg
     6241 ctcaagcacc gtatcgatgg atcgcataca gtgacgatac actgtgtcca tgcgggtcga
     6301 acggccataa agcgctccca atccgtgctt gtttttggga agcggagcaa acggaaactc
     6361 agacgacaca tacgacgtca tttcatccag gatgctatca tcccccgtaa tccggatgta
     6421 ctccccgact ccccaggcaa tccagagcag gttatccgaa gcatgcgaac ggcaagaaaa
     6481 cgccgaccgt ccgtccgtca gagtaaagaa ccagtgaaaa acatcgcctt caataaactg
     6541 ctgagaggcg tgcaaaataa tctgcttgcg cgcgagcgcc ggatccaccc aggtcaggtt
     6601 cactgtgtcc tgaagctgat cccggaaccc gaaagcgccg cttgtctggt aaaaaccccg
     6661 tcgtgcccag atacgctcgg caatggcctg atatttgagc cagttgagat agcggtcaaa
     6721 ttccggctgg ttggtctgga gagtcaccgt ccccatcatc ccaagccacc acgcctttgt
     6781 gctctcgaga gtctttctga ctgtttccat atttttgtac ttctgaacca gttgcaccgc
     6841 ctgccccttc cggtccgctt gtcccagaat aatagcgacc gtgcgttccc cccgggcggg
     6901 aagatcaagc gttcccacaa aagccgcgat ttgggcctgg tcagtcagtt gtgtttcatc
     6961 cggaattgca ttttccacca ggtacgggtc cacggggccc cgtccactgc caaaaaaccg
     7021 tccccgcctg gtctcgaagc aatcggccgg gatcgacatg gaaacaaaag ccggtccgga
     7081 gcgaaaaata tttctcggat tctcgaagaa aagggcgtcc agtgttttgt cgtacttcgt
     7141 taccagaggc cctgaccgtt ccggctgaaa cgcgagcgcc atctgaaaat acggcgccac
     7201 ccgaattttc cgtggccggt tttcatggtt gcgaaccgtt agcagataca cccccatcgg
     7261 atctccggtc gggacaaaca ccgtaagctc cgtcgacaga ttgcctttgg acatacggaa
     7321 aaccgccgtc ccgtccacgc taaattcaca ttcgtttttc gccgcccgat cattcagcgg
     7381 gtggtgggtc ggagaatacc attcccggct ttccggctcg tagaggtaga tggcctcgga
     7441 ggggatttcg cgagtcaccg tatcgggcca gtctggagta agcctgtttt gctgggaatt
     7501 tccattgcaa gacgtatgca acccgcgatt ggtcaccatc acggaatgaa cagggttgga
     7561 cattccatgg tcaaaaggcc gcggcgtgta aggcgttttt acggccagcg tattaccctg
     7621 attcttgaat tcatagtacg gttgaggagt cccttccggg atttcaccgt gtccgatcaa
     7681 gagggaatgt ttccctggtt gaaaatctga tgaaattttc gtgggatcag gctttagatg
     7741 ctttgtcacc atttcaagcg cctgttcttt cttcttcgcg tacccaatca taagacgaac
     7801 agttttcttt tctccggcgg cgagaggcgc gtgaatcaga aaacttccga tcgggtcaaa
     7861 cgtcggacaa gcgggggtat ccttggcttc gagaaaatca agcgtctcga cgattctcgg
     7921 cgcccaaacg cttcgagccc ggccgataaa atccacgcgc ccggtcagaa acccttcggg
     7981 cgcgatgtcg gacgccaaaa accccatgga cttggtgctt ttattccagg aaagcatcgc
     8041 gttggaggca ttaacgtact gcatttcagg gtaaagccgc tgatactgcg tgtggaaccg
     8101 gtcatgcatc cacccgttaa gcacccattc gagatacgga attattttga gtttccgcgc
     8161 ttcgttcgtc ctgttctcca gcgtgacagt ccacagctcg gccgtgctgt cttggtcggg
     8221 caacgtaatt tccaccgagg tctggattcc gtggctcgag tttgtaattc gaaggacatc
     8281 ttcagttttt tcagcttttg agggcgaaaa atacctctcc ggaaaatttc cgcagacagg
     8341 ccaagcccgg tctttgcttt ccggctcctt cgcggtatcc accagaaaaa gaaccctgcc
     8401 acagggatcc acgaggtcat aagagcggcg actcacgtcg cattccttcc cgagcacttc
     8461 tccgtagact tcactgtttt ctttcatggt aacgcggtac tcgcggttcg cgatcgaaaa
     8521 ctcagccact tgtctcttcc tcgatcgccc gcgaaacgcg cggacaatcg aaaccactcc
     8581 ggtcataacc gccaccgaga caacaaggag cagaagagtc tgggaagcgg aaagcgattg
     8641 aatccaggga atcaatccct tcccccgact ggcattctgc atttgttcgg cagtgctcca
     8701 gacatgatcc cattcctgtc cgcgtggcaa ataaaacggg attaaaagcg gagcccatgt
     8761 cgtaaaacgt ttgacacctt cggggatata gcgctgggcc gccgcgggac cgataaatcg
     8821 cgctattttt tcatccagct tgtccatccc gagaaagctc aggttcacga gcgtcgcgac
     8881 ccgaagcccc atgtgatcca tccatatcag gattctgaag aaatcaaacg cgagaatcca
     8941 gacaaagacc gtgagactcc acgcgcggaa atctcccggg gacattgtca gactgttcca
     9001 ggtcgcgaac acggtccgga tggcgccatc ctggttgtac caggttggat ccggcatcat
     9061 gcggaggaac gtatagatga tcggagacat ccagagtccc caccgaagca catagatcat
     9121 caattcggaa agttgcgcca ctcccgcttt ggaaaagaaa aatttgatcg gggtcttatc
     9181 cttgtcgaaa aatgcctgca taaaaacttt gttaaacgcg aaaagccagg tggccacgga
     9241 ccatttaatg accccgtcca gagactccgt aaaaagaagt ttcacgccgc ccggacaaaa
     9301 cccgagatcc gttaatcccc acttgctaat cagcaccggc tgaacataat gccccaggga
     9361 attgaaatag ggataattgt ggtaaacgtc ctgggagaaa cgcgttgtga cgtaaagttt
     9421 aaacttgtag agaacaaccg aaacctgcgg cgcgtcaaga tagaacgcca ccgcgctacc
     9481 aaggaatcca ccaagaagcg aatccaccag ataaagacgc caggtctgga tctttccgca
     9541 accacgcgtc atatagacaa tgtcccggaa aaggctgact ccgccagagg caatcagtcc
     9601 gatgatgaga ccaaacgtaa ccctgtcgga catggccttg tcgatatatc cctggacaat
     9661 catcgcggca aaaccaaaac cgacaaccgc tccccgagcg taaagaaccg gattacggta
     9721 gctaaaagca agacggtcaa aaaacggaag acttccgtcg aagctctcga tgatggtttt
     9781 caccagcgga aacaccaaaa ttcccgcgag aataccaatc agatacggaa acgacatcat
     9841 gatctgctga gccgtatggg tcataaaaac aatggccatc gcgtaaagaa gggtcatcaa
     9901 aattccgctg taggccagac cttttttcat tccagtggaa acatttttaa aaatggtgtc
     9961 gccggaaggc gcgatatgct tgccggaatc aaggaggatt ccggtcagga gataaatttc
    10021 tccccatagg cccgcgaaag agagcatggt agtcacgatt gaaacaagag ccctgcccca
    10081 aagcggccac gtcccaacga ctgccgcgga accaagattt gctatcgaag gcgcgattac
    10141 cacgaacaat gaaacgagaa aaataaaacc gtgggccttg ggttctttga caaaccgtcc
    10201 cttgacaaca ccgctcattc cattggcaag caattcattg aaaataaaaa ctaccagaaa
    10261 acg
//
LOCUS       DAOSIO010000039        10254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_6363_length_10254_cov_5.024218, whole genome shotgun sequence.
ACCESSION   DAOSIO010000039 DAOSIO010000000
VERSION     DAOSIO010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 10254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10254
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6363_length_10254_cov_5.024218"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..763
                     /locus_tag="PK590_02570"
     CDS             257..763
                     /locus_tag="PK590_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23514.1"
                     /translation="MKIFSTFKSDNPAFPFTFEYPQDEWVLLETSGRREIYDEVYLRG
                     KPDSGGEFAPSISIFVRPNEMRKPAEELLSNQLRMEEGLPEFEILKREKVSIDKQKVS
                     LVTFRHKAWVPLGATDGRMVEIKVRMLFFINGEKSYQIVTRTAAEQAVQIFEVFNHIL
                     KTFKFIDR"
     gene            856..2523
                     /gene="dnaX"
                     /locus_tag="PK590_02575"
     CDS             856..2523
                     /gene="dnaX"
                     /locus_tag="PK590_02575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOG23515.1"
                     /translation="MDYLVYARKFRPQTFDEIVGQETVTVTLKNAIKQGRIPQSFLFS
                     GPRGVGKTSTARILAKALNCKEGPTEKPCGKCLACKEIERGNSMDVIEIDGASNRRIE
                     EIRDLRENVKFKPTSGNFKIYIIDEVHMLTLEAFNALLKTLEEPPPHVKFIFATTESH
                     KVPLTILSRCQRFQFKRIPVPEVVKKLESIAKKEKLKCDEKALFLMAKASEGALRDAE
                     SLLDQLASFSGGKITEEDVLTLLGASSDEIYLDVLTAIRERKGADLFALVKKIYEEGG
                     ELAQFAAGLFEVFRHLLVLKVIRETPGFIDASEAMVKGLKKKAADFSEGELLLALSML
                     QNLQYQLRRPVASPKLLVETALLRLMNVGRLKSVEELVGKNKFAPASASTAGSITKPL
                     PEKKTVPPPQKSNEPVKFKKTAGDENSVSPQHSSRDSTESVDLNAVVSVWPQVIESVK
                     AKRMSLGLYLAASEPMEVDDGMIALGFPAEFKFHKETLEKESNRKLIEEMFCQALGKK
                     VRVQCVMTEKDGIHQDLPPETPSTDNSPDIVAQALEIFDGARVIRKE"
     gene            2528..3127
                     /gene="recR"
                     /locus_tag="PK590_02580"
     CDS             2528..3127
                     /gene="recR"
                     /locus_tag="PK590_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOG23516.1"
                     /translation="MRAYSNLMTQLIEALSKLPGIGRRSAERIVFYLLKTRKDETLVL
                     GELILQLRESVTFCSVCHNFSDQKICHICSDPARDQGVICVVEDPKDVSAIEKTGEYA
                     GVYHVLLGALSPLDGIGPEQIMVYDLLDRVKKKKIREIILATNPNTEGDTTALYLARV
                     LAPLKVKVTRIARGIPVGSHIEYTDQATLSRALSGRQPA"
     gene            3124..3921
                     /locus_tag="PK590_02585"
     CDS             3124..3921
                     /locus_tag="PK590_02585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG23517.1"
                     /translation="MIRSVRGLVALVTGASSGIGEAMAYALAEGGADLILVSRDRARL
                     ERISEEIKKRYGVRIWIFETDLTSFSNCESLFQFTETQKIAVDVLVNNAGFAHYGPFE
                     KCEPRDGARMIRLNVESLTVLTRLFLPGMQRRKKGGVLNVASIAGFQPIPYLSVYAAT
                     KAFVLSFSEALWAENLKHGVRVFCLCPGNTRTRFHETAGIMEQKIFFCAPADAVARFA
                     LRKFLKSRRPTAIFGFWNKVMIYAERFGPRALVVFFTNRIYGPSRAR"
     gene            4028..4098
                     /locus_tag="PK590_02590"
     tRNA            4028..4098
                     /locus_tag="PK590_02590"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4060..4062,aa:Gly,seq:tcc)
     gene            4447..5637
                     /gene="nusA"
                     /locus_tag="PK590_02595"
     CDS             4447..5637
                     /gene="nusA"
                     /locus_tag="PK590_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOG23518.1"
                     /translation="MSQDLAASIEYIEKEKGVSREVLMEALRHALLSACRKSFPEKGE
                     GFDVQIDPNTFRIKVFLDGQEVDDPGFGRIAAQTAKQVIIQKLREAERESIFSEYSHK
                     QGDIVSGSVHRIEKKAIIVDFGKTEGILPAREQSPVDNFRQGHLVRAYVMEVKKTSKG
                     TEVLLSRTHPELVRKFFEMEVPEIHDQVVEIKGIAREAGSRSKIAVTSKDEKIDCVGS
                     CVGVRGQRVKNIVRELNGEKIDIIRFSEDAETYIKNAMAPAELQEIKLIPEKRRAEIL
                     VADDQLSLAIGKRGQNVRLASKLIGWELDVRSRSQLLPLTSVEGIGPEIEAALKTAGI
                     HNVRDLSKMKIEDLAKIEGVGEKMAEEILKTARQISIPKSETQEDGEVSQDTEEKPAE
                     ETGL"
     gene            5639..7900
                     /gene="infB"
                     /locus_tag="PK590_02600"
     CDS             5639..7900
                     /gene="infB"
                     /locus_tag="PK590_02600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOG23519.1"
                     /translation="MAEKKEKTKKKVVKKATKPAAEKKTTVKKSVASKTGEKKSSRVA
                     KEKKIAVKPSKEAPVSQVTAPEISGAPAASPIFSDQISEGGETKKDEKKIQPPAPAKT
                     PEPLLKQEEKKIVPPAPAKIPEPVQTPVATPQAAVQPVSEKPGAKTPAPPEPPPVPSV
                     PKPEPVQPKKIEIRFPITVGDLAQKINKRIPEVIKTLMGLGIFANVNQLLNEEIVFAL
                     AEAIGVAIAKEEDEVVEKIQKATSEEDAAKEKLRPPVVTLMGHVDHGKTSLLDSIRST
                     DVAGREKGFITQHIGAYSVDIPGKGHVTFLDTPGHEAFTAMRARGADVTDIVVLVVAG
                     DDGVMPQTIEAIHHAREAKCPIIVAINKCDLPAANPQQVMSGLQKHDLMPEEWGGKTI
                     CVKVSAKTGKGVPELLEMLLLQSEVMELKANPDCPAVGTVVESHLSKGSGPVATVIVR
                     KGTLHHGDMVVAGPYMGRVRVMKNDKGKTLQKAGPAYAVELSGLNGVPEAGEKLYAVE
                     DEKTAKRITEQRMLEIRERQMRGATRHMSLESLYSKISEGIFKELKLIVKGDTQGSVE
                     ALAQSLEKLSTDKCRLHVIHGAVGGINESDVMLAAASDALVIGFHVKADPKAMTMAEK
                     EGVDIRFYGIIYDAVEKIRKAMEGILEPELEEVAEGKAQIRQIFKSSKVGTIGGARVV
                     KGRLLRQRNVRLVRNNVVVFEGKFSSLKHFKDDVKEVAEGHECGISLAGFNDLREGDI
                     VETYRIDKIAATL"
     gene            7993..8349
                     /gene="rbfA"
                     /locus_tag="PK590_02605"
     CDS             7993..8349
                     /gene="rbfA"
                     /locus_tag="PK590_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOG23520.1"
                     /translation="MQGKRTERVGSLLQMELSKLILERVKDPRLGFVTITHVKVTADL
                     KSAVVFFTVLGDQGKKEETLNVLKRAAGFLQREIGQFLEMRYTPRLQFELDDMVDKSF
                     EIDEVIKKIENPPPKA"
     gene            8353..9081
                     /gene="truB"
                     /locus_tag="PK590_02610"
     CDS             8353..9081
                     /gene="truB"
                     /locus_tag="PK590_02610"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007277178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOG23521.1"
                     /translation="MPPPPEFIDGILLIDKPTEWTSHDVCAFVRKRFKFKKVGHAGTL
                     DPLATGLLVVLIGKATKYSMALSSCDKEYCGTLELGVQTDSHDRQGNVIAESACDHIT
                     LERVREEAKAFTGEILQVPPMVSALKHKGTRLYKLARQGKTVPREGRKVTVHEFRFDK
                     QEGRFINFSTHVSKGTYVRTLVNDLGEALGCHACLAGLRRIRSGQFRIEQSVTMEALR
                     NMTVDEISGKMFPLHTVSGHATHH"
     gene            9065..10069
                     /gene="ribF"
                     /locus_tag="PK590_02615"
     CDS             9065..10069
                     /gene="ribF"
                     /locus_tag="PK590_02615"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HOG23522.1"
                     /translation="MRLITDLRAYRTRNLQRLVLAIGNFDGFHLGHQKILAYVVKQAR
                     KNHALSAVLTFRNHPQVVLHPQKKKLLLFSFDQKLAFLQKAGVDICLAPFFTRVFSKM
                     SPEVFVEKILAKQLRVLEVCMGVDARFGYRRRGTTSLMENLARKYGFLFKRMEPVLIG
                     KRPVSSSWIRELLVEGEIEKVNRCLGRPFSIWGDIVRGKGHGIHLGFPTANLESYANI
                     QIPLGVYVASARFLRRKMSFATDSDDSTRFFKEVSPWIPGVMNFGKRPTYPSSETPQP
                     VLETHLFDFKKRVYGKTMEVALHKFIRPEKRFADEGVLKKQIGKDVEVARKWHRKRPR
                     "
BASE COUNT         2711 a         2282 c         2802 g         2459 t
ORIGIN      
        1 cttttaacat tcaggcgtct catttcatca acgttttgac gggagaatta gtgccggaaa
       61 gtacagcaaa agcttatgtt aaaagccatg acgtttacgc taagttaccc gactattaaa
      121 tgtgtttcga aaggatgatc ctatgtgttt tttgtcaaaa aataaactct ggataatcgg
      181 cgcaggcgtt cttattatct ccttgtttta tttttatgtt gataacaaca cgaataacac
      241 caacaaggat tttttggtga aaatattttc cacttttaaa agcgataatc ctgctttccc
      301 ttttaccttt gagtatccgc aagatgaatg ggtcttattg gaaacaagtg gacgacgcga
      361 gatttacgat gaagtttatc ttcgaggtaa gccggattct ggaggagagt ttgcgcccag
      421 catctctatt tttgtccgcc ccaatgagat gaggaagccg gcggaagagc tcctttccaa
      481 tcagttaagg atggaagaag gattgccgga atttgaaatt cttaaaagag agaaggtctc
      541 cattgataaa cagaaggttt ctctcgttac tttccggcat aaagcttggg ttccgcttgg
      601 tgctactgat gggcggatgg ttgaaataaa ggtaagaatg ttatttttta ttaacggtga
      661 gaagtcttac caaattgtaa cacgcacagc cgcggaacag gccgttcaga tttttgaagt
      721 ttttaatcat attttaaaaa cgttcaaatt tatagaccga taaatatttt actgataaga
      781 acttcttgaa aacactgttc ttttcccgca tcatacagtt cttctgatac ttctcaacgg
      841 caaccaagga ttttcatgga ctacctcgta tacgcccgta aatttaggcc gcaaaccttc
      901 gatgaaatcg tggggcagga gaccgtcacg gtcacgctca agaacgcgat taagcagggg
      961 cggatccctc agagtttcct tttttccggt ccccggggcg tgggaaaaac ttccacggcc
     1021 cgcattttgg ccaaggcgct taactgcaaa gaaggcccca cggaaaagcc ttgtggaaag
     1081 tgtctggcct gcaaggaaat cgagcggggg aactcgatgg atgtgatcga aattgacgga
     1141 gcgtccaacc gccgcatcga agaaatcagg gatttgcgcg aaaacgtaaa attcaagccg
     1201 acgtccggaa atttcaaaat ctacattatc gatgaagtcc atatgctgac tctggaagcg
     1261 tttaatgcgc tcctgaagac cctcgaagaa cctccgcctc acgtgaaatt tatttttgcg
     1321 acgacagaat cacataaggt gcctcttacc atcctttcgc gttgccagcg gtttcagttc
     1381 aagagaattc cggttccgga agttgtgaaa aaacttgaat cgatcgccaa gaaagaaaaa
     1441 ctcaagtgtg acgagaaggc tttgtttctg atggccaagg ccagtgaagg cgcgttgcgg
     1501 gatgctgaaa gcctgctgga ccagctcgcg agtttttcgg gcgggaaaat cacggaagaa
     1561 gacgttctca ctctcctggg ggcttcttcg gatgaaatat atcttgacgt tctgacggcc
     1621 attcgtgaga gaaaaggggc ggatttgttc gccctggtaa aaaaaatcta cgaagaaggc
     1681 ggcgagttag cccagttcgc ggccggtctt tttgaagtct tcaggcatct tctggtactc
     1741 aaagtgatac gcgagacccc cgggtttatt gatgcgagtg aggcgatggt gaaaggcctt
     1801 aaaaaaaagg cggctgattt ttcggaagga gaacttctac tggccttgtc catgcttcaa
     1861 aatcttcagt atcagttgcg acggcccgtg gcgtcaccca aattacttgt tgaaaccgcg
     1921 ttgctcaggc tcatgaacgt cggtcgtctc aagtcagtcg aggagcttgt gggaaaaaac
     1981 aaatttgccc cggcatcagc ctcgacggcg ggttcaatca caaaaccctt gcctgaaaaa
     2041 aaaacagttc cgccgcctca aaaatcaaat gaaccggtaa aatttaaaaa gacagcgggg
     2101 gatgagaatt ctgtatctcc tcaacactcc tccagagatt cgacggagtc ggttgatctg
     2161 aatgccgtgg tatcggtgtg gccgcaggtc atcgaaagtg tgaaagcgaa acggatgtcc
     2221 ctggggcttt atcttgccgc gtccgaacct atggaagtag acgacggaat gatcgcgctt
     2281 ggatttcccg cggagtttaa atttcataaa gagactcttg aaaaggagag taacaggaag
     2341 ctgattgaag aaatgttttg tcaggcgctc ggtaagaagg ttcgcgttca atgcgtgatg
     2401 acggaaaaag acgggatcca tcaggatctc ccccccgaga ccccttctac ggacaattca
     2461 ccggacattg ttgctcaggc ccttgagatc ttcgacggcg cccgcgtgat tcgcaaggaa
     2521 tagtgtgatg cgagcctatt cgaatctgat gacccagctt atcgaagctc tttccaaact
     2581 tccggggatc ggtaggaggt cggcggagcg cattgtgttt tatcttctca aaaccagaaa
     2641 agacgaaact ttggtgttgg gggaactgat cctccagctt cgggaaagcg tcactttttg
     2701 ctccgtttgt cataattttt cagaccagaa aatttgccac atatgttcgg atcctgcccg
     2761 tgaccaggga gtgatttgcg tggtcgaaga tccgaaagat gtttccgcga tcgaaaagac
     2821 cggtgaatat gccggcgtct atcatgtttt attgggggcg ctttctccgc tggacggtat
     2881 tgggcctgaa caaattatgg tttacgatct tctggaccgc gttaaaaaga aaaaaatccg
     2941 tgagattatc cttgccacga atccgaacac tgaaggcgat acgaccgccc tttatcttgc
     3001 gcgggtgctc gcgcctctga aagtgaaagt gacccggatc gcgcgtggaa ttccagtggg
     3061 cagtcatatc gagtacacgg atcaggcaac tctttcccgg gcgctttccg gacgccagcc
     3121 cgcatgatcc ggtccgtccg tggtttggtt gctcttgtca ccggggcgtc ttcaggtatt
     3181 ggggaggcca tggcttacgc cttggcggaa gggggagcgg atcttatttt ggtctcacga
     3241 gacagagcga gactggagcg aatatcggaa gagattaaga aacgttatgg agttcggatc
     3301 tggatttttg aaactgatct gacaagtttt tccaactgcg agagtctttt ccagtttact
     3361 gaaacacaga agatagcggt tgatgtgctt gtgaacaatg ccggttttgc ccactacggc
     3421 ccatttgaaa aatgtgagcc gcgggatggg gcccggatga ttcgcttgaa cgttgaatcc
     3481 cttaccgttt tgactcgcct ttttttacca ggaatgcaac ggaggaaaaa ggggggcgtg
     3541 ctcaatgtcg cctcgatcgc cggctttcaa cccatcccct acttatccgt ctatgcggcg
     3601 accaaggcgt ttgttctgag tttttccgaa gcgctctggg ctgaaaacct gaaacacgga
     3661 gtccgtgttt tttgtctctg ccccgggaat acgcgtaccc ggtttcatga aacagcgggg
     3721 attatggaac agaaaatatt tttttgcgcg ccagccgacg cggtcgcccg gttcgctctc
     3781 cgcaaatttc tgaaaagtcg aagacctacc gctatctttg gtttctggaa taaagtaatg
     3841 atttacgcgg agcggtttgg gccgagagct ctggtggtct ttttcacgaa ccggatttac
     3901 ggcccgtctc gcgcgcgata gaccgatcta aacctgttct cggatgacaa aattagcgtc
     3961 atgagaaagt tgcatgtcgc tctattcttg attagaatgt cggccgtttc atttcgctga
     4021 caagcacgcg ggcatagctt aatggtaaag tcccagcctt ccaagctggt catgcgggtt
     4081 cgattcccgc tgcccgctga tttcaggtag acttccctca taatgttggg ccctccttcg
     4141 aaaattccta tgtatatcaa tatgtccgga aggcgtgaga aaacgccaat ctgaagaaca
     4201 aagactgaag ccgaatccgg acactgcccc gaaggttgga tttttgtgta gtttgaatga
     4261 caaaacccta cggcataaaa aaacgcaacc cttttatttt caataagtta cgagaaaacg
     4321 aagcaaaccc gccgttgact tttaaaactt tccccctata atacgccttg ttaattttgg
     4381 ttattgcgaa taagtgggca aaaacccact ttttttttct aaacaggtaa tgataggtag
     4441 gacaaaatgt cacaggacct tgcggcgtca atcgaataca tagaaaagga gaaaggggtt
     4501 tccagggaag ttcttatgga ggcccttcgg cacgctctcc tgtcagcttg ccggaaaagc
     4561 tttcctgaaa aaggggaagg ttttgatgtc cagattgatc cgaatacgtt ccggatcaag
     4621 gtgtttctgg atggccagga agtagatgac ccgggatttg gacggattgc cgcccagacg
     4681 gccaagcagg tgattatcca gaaacttcgc gaagcggagc gggaatctat tttttcggaa
     4741 tacagccaca agcagggaga tattgtctcc ggcagtgtgc accggattga aaaaaaggca
     4801 attatcgtcg attttggaaa aacggaaggg attctccccg cccgtgaaca atccccggtt
     4861 gataatttcc gccaggggca tttggtcagg gcttacgtga tggaagtcaa aaaaacatcg
     4921 aaggggaccg aagtcttgtt gtcccgcact caccctgagt tggtccggaa attctttgag
     4981 atggaagtgc cggaaattca cgatcaagtg gttgaaatta aggggatcgc gcgtgaggcc
     5041 ggatcccgaa gcaaaatcgc cgtgacatcc aaagatgaaa aaatagattg cgtgggttcc
     5101 tgtgttggtg tgcggggaca gcgcgtgaag aatatcgtgc gggagctgaa cggagaaaag
     5161 atcgacatca tccggttcag cgaggatgcc gagacgtaca tcaagaatgc catggcgccc
     5221 gcggaactcc aggagattaa actgatcccc gagaaacgtc gcgccgaaat tttggtcgcg
     5281 gacgatcagt tgtcgctcgc gatcggaaaa cggggacaga acgttcgcct tgcctccaag
     5341 ctgatcgggt gggagctgga tgtgcgcagc aggtcccagc ttttgccgct cacgtcggtt
     5401 gaggggattg ggccggaaat tgaagccgcg ctcaaaacgg ccggaatcca taatgtgaga
     5461 gacctttcga aaatgaagat cgaagacctc gccaaaatcg agggggtcgg agagaagatg
     5521 gccgaagaga ttttaaagac ggcgagacag atttcaatcc cgaagagcga gacccaggaa
     5581 gacggtgagg tttcgcaaga tacggaagaa aaacccgcgg aagaaacggg gctttaagat
     5641 ggcagaaaaa aaagaaaaga caaaaaaaaa ggtagtgaag aaggcgacaa aacccgcggc
     5701 cgagaagaag acgacggtca aaaaatcggt cgcttcaaag acgggtgaga aaaaaagctc
     5761 gcgggtggcc aaagaaaaaa aaatagctgt caagccctcc aaagaagcgc ccgttagcca
     5821 agtcacggcc cctgaaattt caggggcgcc ggccgcttcc ccgatctttt ctgatcagat
     5881 ttcggaaggg ggcgaaacga agaaggatga gaaaaagata caacctccgg ctccagcgaa
     5941 aacgccggaa cctctcctca aacaggaaga gaaaaagata gttcctcccg cgcccgctaa
     6001 aattccggaa cccgttcaaa cgcccgttgc cacgcctcag gcggcagttc aaccggtttc
     6061 tgaaaaaccc ggggccaaga cacccgcgcc tccggaacca ccgccagtac cgtccgttcc
     6121 taaaccggag ccggttcagc ccaaaaaaat tgaaatccgt tttccgatca cggttggcga
     6181 tcttgcccaa aaaataaata aaaggattcc tgaagtcatt aaaactttaa tggggctggg
     6241 aattttcgcg aacgtaaatc agttgttgaa cgaggagatt gtgttcgcgc tcgcggaggc
     6301 cattggggtc gcgatcgcga aagaggaaga tgaagtggtt gagaaaattc agaaagccac
     6361 ttcggaagaa gacgccgcga aggagaagct tcgcccgccc gtggtgactt tgatggggca
     6421 cgtggatcat ggaaaaactt ccctgcttga ttcgattcgc agtacggatg ttgccgggag
     6481 agaaaaggga tttattactc agcacatcgg cgcttacagc gtcgatattc ccgggaaagg
     6541 tcatgtgacg tttcttgata ctccggggca cgaggctttt actgccatgc gggctcgtgg
     6601 cgccgacgtg acggacattg tggtgcttgt ggtggcggga gatgacggcg tgatgcctca
     6661 aacgatcgag gcgattcacc acgcccgtga ggcgaagtgt ccgattatag tcgccatcaa
     6721 taaatgtgat ctccccgcgg ccaatcccca gcaggtgatg tccgggcttc agaaacacga
     6781 tttgatgccg gaagaatggg gtggaaagac catttgcgtc aaggtgtcgg ccaaaacggg
     6841 aaaaggagtt ccggaacttc tggaaatgtt gctactgcag tccgaagtga tggagctaaa
     6901 ggcgaaccct gattgtccgg cggtgggaac ggttgttgaa agccatttgt cgaaagggag
     6961 tggccctgtc gcgaccgtga ttgtgcggaa ggggacgctt catcatggcg acatggtcgt
     7021 ggccggcccc tatatgggac gagttcgggt aatgaaaaat gataaaggga agactcttca
     7081 aaaggcggga ccggcttacg cggttgaact tagcggactg aacggtgtcc cggaagccgg
     7141 cgaaaagctt tatgccgttg aagatgagaa aacggcgaaa cggattactg agcaaagaat
     7201 gcttgaaatt cgtgagcggc agatgcgtgg ggccactcgc catatgtcgc tggaaagtct
     7261 ttattcgaaa atttcggagg gaatcttcaa ggaattgaag ctgatcgtta aaggtgatac
     7321 gcaaggatct gtcgaggcgc tggctcagtc gctcgaaaag ctcagtacgg acaaatgtcg
     7381 gttgcatgtc attcatggcg cggtgggtgg cattaacgaa tcggacgtga tgctcgcggc
     7441 ggcttcggac gcgcttgtga tcgggtttca tgttaaagca gacccgaagg cgatgacgat
     7501 ggcggaaaaa gaaggagtgg acattcgctt ttacgggatc atttatgacg cggttgaaaa
     7561 aatccgtaaa gccatggaag gaatcctgga gccggaactc gaagaggtcg ctgaaggaaa
     7621 agcgcaaatc cgccagattt ttaaatcctc caaggtagga accatcgggg gggcacgggt
     7681 ggtcaaagga cgtctcctca ggcaacgcaa cgtgcggctg gtccggaata acgtagttgt
     7741 ttttgaagga aagttttcct ccctgaaaca tttcaaggat gacgtcaaag aagtcgcgga
     7801 aggccatgag tgcggtattt ctctcgcggg atttaatgat cttcgtgagg gtgatatcgt
     7861 ggaaacctac cggattgaca agattgccgc gaccctctaa acatctgagg agtcgggaaa
     7921 gtttccgact cattcaccct tttgggttcg tgttttgtat ttttaatttt aatttatgtc
     7981 aaaggatgag caatgcaagg aaagcgtacg gaacgcgtgg gttcgttgct tcagatggaa
     8041 ttgagcaagc tcattctgga acgagtgaag gatccgcgtc ttggttttgt caccatcacg
     8101 cacgttaaag tgacggcgga cctgaaatcg gcggtcgttt tttttacggt tttgggcgat
     8161 caggggaaaa aagaggagac cttgaatgtc ctgaagcggg cggccggatt tctccagcgt
     8221 gaaattgggc agttcctcga gatgcgatac acgccgaggc ttcagtttga acttgatgac
     8281 atggtcgaca aaagttttga gattgatgag gtcataaaaa agatagaaaa tccgcctccc
     8341 aaggcctagc ttatgccgcc accccctgaa tttattgacg gaattcttct tatcgataag
     8401 cccaccgaat ggacatctca cgatgtttgc gcctttgtcc gaaagcggtt taagtttaaa
     8461 aaagtaggtc atgctggcac tctggatccc cttgcgaccg gtcttctcgt ggttttaatc
     8521 ggcaaggcca ccaaatattc gatggcgctg agctcttgcg ataaggagta ttgcggaacc
     8581 ctggagttgg gcgtccagac tgactcccac gaccggcagg ggaatgtgat tgcggagtcg
     8641 gcctgtgacc acatcactct tgaaagagtc agggaagagg cgaaggcgtt tacaggcgaa
     8701 attctccagg tgccgccgat ggtctccgcc ttaaagcata aggggacgag gctttataaa
     8761 cttgcgcgac agggcaagac tgttccccgg gagggacgaa aagtgacggt gcatgagttt
     8821 cgttttgaca aacaggaggg aagattcatc aatttttcca cccatgtgtc caaagggacc
     8881 tatgtgagaa cgcttgtcaa tgatctcggt gaggctctgg gttgtcatgc ctgccttgcc
     8941 gggctaaggc gaatccggag cggccaattt agaatcgaac aaagcgtcac gatggaagcg
     9001 ttacgcaata tgacggttga tgaaattagt gggaagatgt ttccgctcca cacggtttcg
     9061 ggccatgcga ctcatcactg atttgcgcgc ttatcggaca agaaatctcc agcgccttgt
     9121 tctggcgata ggtaattttg atgggtttca cctcgggcat cagaagattc ttgcttatgt
     9181 ggtcaagcaa gcccggaaga accacgcgct gtcggctgtc ttgactttcc ggaatcatcc
     9241 ccaggttgtt ctccatcccc aaaaaaagaa attgcttctt ttttcttttg atcaaaaact
     9301 cgcgtttttg caaaaggcgg gagtcgatat ctgtctggcg ccttttttta cgcgagtttt
     9361 ttccaaaatg tctccggaag tttttgtgga gaaaattctt gcgaagcagc tccgggtcct
     9421 tgaggtttgt atgggagttg acgcgcgctt cggctatcgg cgtcggggga cgacgagttt
     9481 gatggagaat ttggcccgga aatatggatt tctgttcaaa cgaatggaac ccgtcctgat
     9541 cgggaaaagg cccgtttcaa gttcatggat ccgggaactt ttggtggaag gagagattga
     9601 aaaagtgaac cgctgtctcg gacgcccgtt cagcatttgg ggggatattg ttcgggggaa
     9661 gggccacggg atccacctcg gttttccgac ggcaaatctg gaatcttatg ccaacataca
     9721 gattccgcta ggggtttatg tggcttccgc gcgattttta cggaggaaga tgagctttgc
     9781 cacggattct gatgattcaa cccgtttttt taaggaagtt tctccatgga ttcctggcgt
     9841 catgaacttt ggaaaacggc cgacttatcc ttcgtccgaa acaccccaac cagtgcttga
     9901 aacgcacctt tttgatttta agaagcgggt ttacggaaaa accatggaag tggctcttca
     9961 taagtttata cggcccgaga aacggtttgc cgatgaaggt gttttaaaga agcaaatcgg
    10021 caaggatgtg gaagtggccc gaaaatggca ccggaaacga ccacgatgat tgtttttaag
    10081 aaactcttta caaacccatg ggtgcggggt atacttccgc aacctcagag atgagagaaa
    10141 cgacataact caaagagaga gaaggagtta caaagacgca tgctagcgaa gacgaagaaa
    10201 aaagagatca ttgccaagaa caagaaaacg aagaacgata ccggatccgc cgaa
//
LOCUS       DAOSIO010000040        10235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_6376_length_10235_cov_4.497839, whole genome shotgun sequence.
ACCESSION   DAOSIO010000040 DAOSIO010000000
VERSION     DAOSIO010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 10235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10235
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6376_length_10235_cov_4.497839"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PK590_02620"
     CDS             <1..258
                     /locus_tag="PK590_02620"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOG23523.1"
                     /translation="DSNIGDNVNVGAGVITANFDGKNKRPTRVGKNVFLGSNTVLVAP
                     VHVPTCVKTGAGAVVTRKTRIRKNDIVVGVPAKSVSQKKRR"
     gene            263..1252
                     /locus_tag="PK590_02625"
     CDS             263..1252
                     /locus_tag="PK590_02625"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOG23524.1"
                     /translation="MTKIRIHKKNNGSMVLLTGGANPALGQEIAKLLGIRTGNVTLGR
                     FPEGEIQVQIHDNIRGKDVFIIQPTCTPPNESLMELLILIDAARRASAKRITAVLPFF
                     GYARQDRKDRPRVPITAKLVANLIVSAGANRVLTVDLHAGQIQGFFDIPVDHLYSIAV
                     LGAYFKKKKLKNLVVVSPDVGGIKMARGYAKLFGCDLAVVDKRRESASTTHVMHIIGS
                     VAGKNVVLLDDLISTGGSIVEAAKALKAHGAKDIYACIVHPVLAGSAIERIKNSPIKE
                     LIVTNSIPLTKEKRISKITQLSIAPLLADAIWRIHNDVSISTLFFNNELGVEA"
     gene            1405..2103
                     /locus_tag="PK590_02630"
     CDS             1405..2103
                     /locus_tag="PK590_02630"
                     /inference="COORDINATES: protein motif:HMM:NF004128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOG23525.1"
                     /translation="METFELQVKPRTVEGSRKARNVRRQELIPAIVYGHGMKPLALEI
                     PEREFLKAIQTKAGENVMISLKVEGVTLQESTCLIKAMQHDPVTDQISHVDLMMISMT
                     EKITVKVPVVVVHGDEAPGVKEGGALDVVHYEIEVECLPTQIPEKIEIDVKDMKINDV
                     VHVSQLVIAEGVQCLLDPEDVVVAIRPPREEEVAAPAEDGPAQPEVIEKGKKDKEGED
                     GEAPKASAPAKETK"
     gene            2157..2768
                     /gene="pth"
                     /locus_tag="PK590_02635"
     CDS             2157..2768
                     /gene="pth"
                     /locus_tag="PK590_02635"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOG23526.1"
                     /translation="MKLIIGLGNPGQEYKKTRHNMGRRLAEFLAEKEGRKFRRLKSLE
                     AHVAEMSWENAPSVVVAYVETYMNLSGKRIRPLFDHYEVSDLQDLLIVVDDIALPFGK
                     MRLRAGGSAGGHNGLTSIEEAFQSAEYPRLRLGIGIQHEEHPERSAGTGTYEPVRDYV
                     LSEFSSAEEKDMTAFLERGAESCRRWARGPFEKAVQWVNSAKS"
     gene            2932..3255
                     /gene="rpsF"
                     /locus_tag="PK590_02640"
     CDS             2932..3255
                     /gene="rpsF"
                     /locus_tag="PK590_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOG23527.1"
                     /translation="MKKYEGLFIFPPEDKDQALEVTKWIEKFQGKVLQKADLGKRTIG
                     YQIRKFRDGKFLVFNFEMDPSKVQDFRKDLEQQETLLKYMVTVLREESRPSVSTATSQ
                     TTPQS"
     gene            3272..3751
                     /locus_tag="PK590_02645"
     CDS             3272..3751
                     /locus_tag="PK590_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOG23528.1"
                     /translation="MAANLNKVMLIGNLTRDPELRYLPSGQAVTTFSIACNRTYVAKT
                     GEKKEEVSFIRIVVWARRAEVCNEYLKKGSAVFVEGRLQSRSWDAPDGSKRSAVEVIA
                     DNVQFLSRGGGAKSSDQADVPSMDVDDSIFEAPDASGKKNQASVSQDELKPDEDIPF"
     gene            3888..4082
                     /gene="rpsR"
                     /locus_tag="PK590_02650"
                     /pseudo
     CDS             3888..4082
                     /gene="rpsR"
                     /locus_tag="PK590_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012594993.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
     gene            4098..4544
                     /gene="rplI"
                     /locus_tag="PK590_02655"
     CDS             4098..4544
                     /gene="rplI"
                     /locus_tag="PK590_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOG23529.1"
                     /translation="MELLLIKDVFDVGKKGDVVKVREGYGRNFLLPRNLAIPATRKNQ
                     QFFEEQRARSAARKLKEKTAAEKKAKELAELTITLEVKAGEGDKLYGSVGPEEVCAAL
                     VQKGYEFDKKQIRMREHLKNLGTHHVDLEIYPQVKTRLTVELVRQP"
     gene            4571..5938
                     /gene="dnaB"
                     /locus_tag="PK590_02660"
     CDS             4571..5938
                     /gene="dnaB"
                     /locus_tag="PK590_02660"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOG23530.1"
                     /translation="MANSETSVKLYEKVPPQNREAEMSVLGAMFFDEMALAKGIEILR
                     PEYFYDLRHQRIFDVVAGLFERNQAVDLITASEELRKRDQLEDVGGVSYLTQLTMAVP
                     TAANIEYYAKIVKEKALLRQLIQSATQIVQNSLESDSNAKEMIDTAEKMIFDIGQAQV
                     EGKSFQIKEIIHESMEAIDQLFQKKQHVTGLATGFHEFDTMTAGLQPSDLIIVAGRPS
                     MGKSAFATAIVEHAGINLKKPVVIFSLEMSKEQLVQRMLCSHARVDAQKVRTGYLSHQ
                     DWPKLTSAAGKLSEAPIFIDDSPGQTVLEIRAKARRLKMKHDIQLIVIDYLQLMQGVC
                     VAESRQQEISEISRSLKALAREIKVPVIAVSQLSRAVEQRTGNRPQLSDLRESGAIEQ
                     DADLVVFLFREEYYAPSEENKNKAESIIAKQRNGPTGSVPLVFIKEWTRFDNPEFYRS
                     DEEQQ"
     gene            5935..8265
                     /gene="bamA"
                     /locus_tag="PK590_02665"
     CDS             5935..8265
                     /gene="bamA"
                     /locus_tag="PK590_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOG23531.1"
                     /translation="MKRIGCLILAITLMMTSAPLHGSEELVASSADSPAPQAPAEAKK
                     IAAIEVRGNKIVSTATILSKIQSQPGSPLRQQLTNEDIKRLYDTGFFQDVRFDVTEIA
                     DGYKLIVQVDEKPIIRRIEIQGNTVFKEDKLRKTLGVMEGQILDQKAVKKGETEIRQL
                     YANKGFRFIDIQTEIVTDPQTRESIVTMKIFEGKKFKIKEVRFEGNQSFSSKQLAKLM
                     KTRPQMWVLFGTFKEEVFEKDLERISFHYQKEGYLDVKVVPEFDYLKEKNQINILIKI
                     EEGQHYVTGEVRFKGNTLFPESELWQELEMLPGLTYSQFYVSQDMESIRNFYASEGYI
                     EARVAPDVVLNRETGKVDVTYEIAEGELFFVEKVQIRGNTKTRDKVIRRELRIRPGER
                     FDGAKIEKSKQRLENLGYFEEITYDTEPTDVATNRRDLIFRVKEKRTGELSFGGGVSS
                     IDTFMGFAEISQRNFDLFNWPRLTGGGQTLSLRGQIGTINQQYDFNFIEPYLFDKPIT
                     MDFNTYYTVRDDYNVDFKEKRFGISEIFSHLFKDVFRVGGGYVLERVKLDDIASDAPQ
                     DVLDFAGKNWLSRVRTFAVYDNRDNVFNPTKGRTVNVGADLVGSFLGGDQDYYILRAG
                     YTEYMSLRKNHLVEFQARIATAQDFGDSTSVPVFDRFYAGGLGTVRGYGYRRVSPKQN
                     DNPVGGQTMAIINLDYSFPIPKLDMFRGVVFADAGMVNKSSYDLSFGDVVVSVGPGIK
                     VKTPIGPLAFYYGFPLVNKDTEDENGRFEFSLSRSF"
     gene            8293..8802
                     /locus_tag="PK590_02670"
     CDS             8293..8802
                     /locus_tag="PK590_02670"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOG23532.1"
                     /translation="MKKIVSALFFAGLMTWLASPAVAVEMKIAYVAVDEVFDNYQKTK
                     DQDLVLKTAGEAKERERNEMMNALRAMEDELALLSADSRAKKQEQLIEKKRQIEDFDR
                     TVRQQLAEQRDKTVREIFEEIDSVVQVVCERGGYDLVFNKRVLLFQKSNFDITSQVSA
                     ELAKRYKKA"
     gene            8814..10097
                     /gene="lpxC"
                     /locus_tag="PK590_02675"
     CDS             8814..10097
                     /gene="lpxC"
                     /locus_tag="PK590_02675"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOG23533.1"
                     /translation="MKQRTLKKSIHFEGIGLHTGQKVSVRINPAAENSGILFSRMDLD
                     PALKVKASFENVRSDAMRQTALAVGSGLVRTIEHLMAAFHGLGVDNAWVEIDGEEVPG
                     MDGSSQEFSRLLLEAGFEEQGAERKYLEVKSPVYVDHGDQSIVIVPAQSFQVSYTLSY
                     QDENLEDQYMTITVLPEIFERELAPARTFCLKKEAEQLLAMGFGKGANPLNTLIFENN
                     RPIGNELRYANEACRHKIVDILGDLFLCGKFIRGHVIAQRSGHSLNLELVRKLVMTKE
                     TSLDLSSKKTLTIDEIQKIIPHRYPFLFVDKIENFEPGLRATGWKQVTMNDYFFQGHF
                     PGHPVMPGVLIVEAMAQVGGVIMLGEPENRGKIAYFMSVDQVKWRNPVRPGDSLRMEV
                     EVLKKRSKFGQCSGKAYVGDQLVCEADVKFAVVDA"
     gene            10058..>10235
                     /locus_tag="PK590_02680"
     CDS             10058..>10235
                     /locus_tag="PK590_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23534.1"
                     /translation="MRSGCEIRRGGCLKIHPTAIVHPDAILGEGVEIGPWAIVEGTVK
                     VGARTKVGPRVTIEG"
BASE COUNT         2692 a         2184 c         2876 g         2483 t
ORIGIN      
        1 gattcgaaca taggggataa cgttaacgtc ggagcaggcg tcattacggc caattttgac
       61 ggtaagaaca agcgtcctac ccgggtgggg aaaaatgttt ttttaggttc taatactgtt
      121 ttggtggcac ccgttcatgt gccaacttgc gtgaagaccg gggctggtgc cgtggtgacc
      181 cgtaaaacac gaatacgaaa gaacgatatt gtggttgggg ttccagcgaa atcagtttct
      241 caaaaaaaga ggcgatgact ttatgacaaa aatcaggata cacaagaaga ataacggttc
      301 tatggtgttg ctgacggggg gggccaatcc cgcgctaggg caggaaatag caaaacttct
      361 cgggattcga acggggaacg tgacactggg acgatttccg gaaggagaaa ttcaggtcca
      421 aatacacgat aacattcggg gaaaagatgt ttttatcatt caaccgactt gtacaccacc
      481 caatgaaagc ctgatggaac ttttgatttt aatcgatgcc gcccgaaggg cttccgcgaa
      541 gcgaattacc gcggtgttgc ccttttttgg ttatgcgcgg caagatcgta aagaccggcc
      601 tcgtgttccg attaccgcaa agctggtcgc aaacctcatc gtcagtgcgg gcgccaatcg
      661 cgtattgacc gttgatcttc atgccgggca gatccagggg ttttttgata ttcccgtgga
      721 tcacctttat tccattgccg ttctgggagc ctatttcaag aaaaagaaat tgaagaatct
      781 ggtcgttgtt tctccggatg ttggcgggat caaaatggct cgagggtacg cgaagctttt
      841 cggatgcgat cttgcggttg tggataaacg ccgcgaaagc gcgtcgacga cccatgtgat
      901 gcatattatc ggttctgtgg ccggcaagaa tgtggttctg ttagacgatt taatctcgac
      961 gggggggtca atcgtggaag cggcgaaggc tctcaaggcg cacggagcga aggatatcta
     1021 cgcgtgcatt gtccaccctg tgttggcggg aagcgcgatc gagagaatta aaaattcgcc
     1081 catcaaggaa ttaattgtga cgaacagtat tcctctgacg aaggaaaaaa ggatttccaa
     1141 aattacccag ctctcaatcg ctccgcttct ggccgacgcg atctggagaa tccataacga
     1201 tgtttctatc agcaccctgt tttttaataa tgaattgggt gtggaagcgt agcggtcacg
     1261 cgccttcgtt cgtaagacgt cgggttcgtt ttaacgttcc tctggttgaa attcataaag
     1321 aagggtgatt ttttctgaaa cagtgaacca taacagctcc tgtagggagt caagactcaa
     1381 acgggacaag gaggatttac attcatggaa acttttgaac tgcaagtgaa accccggaca
     1441 gtggagggga gtcggaaggc ccgcaacgtt cgacgtcagg aactgatccc cgcgattgtt
     1501 tacggccacg ggatgaagcc gctggcgctg gagatacccg agcgggaatt tttaaaagcg
     1561 attcagacca aagcgggtga aaacgttatg atttcgctca aggtggaggg ggtgactctt
     1621 caggagtcca cgtgtctgat caaagccatg caacacgacc ccgtgacgga tcagatcagc
     1681 catgttgatc ttatgatgat ttccatgact gaaaagatca ctgtaaaagt tccggtggtt
     1741 gttgttcatg gcgatgaggc cccgggcgtc aaagagggtg gcgcgcttga cgtggttcac
     1801 tatgaaatcg aggtggagtg tttaccaacc caaatccctg aaaagatcga gattgacgtc
     1861 aaagacatga aaattaatga cgttgtccac gtcagtcaac ttgtgattgc cgaaggagtt
     1921 caatgccttc ttgatccgga ggatgtggta gtcgcgattc gtccgcctcg cgaagaggaa
     1981 gttgcggcgc ccgcggaaga tgggcctgct cagccggaag tcattgaaaa gggtaagaaa
     2041 gacaaagaag gggaagatgg cgaggcccct aaggcttcgg ctccggcaaa agaaacaaaa
     2101 taataaaaaa ggccggcgaa cgtgttttct tgtttgccgg ttcttttttt atcgacatga
     2161 agcttattat tggtcttgga aatccagggc aggaatacaa gaaaacaaga cataacatgg
     2221 ggcgacggct cgcggagttt ttggccgaga aagaaggtcg caagttccgg cgtttgaaat
     2281 cgctcgaggc ccatgtggcg gagatgtcgt gggagaacgc tccatcggtg gtggtcgcgt
     2341 atgttgaaac ttacatgaat ctttcgggga aacgaattcg acctctattc gatcattacg
     2401 aagtttcaga tttgcaagac cttctgatcg tggtagatga tattgccctt ccgtttggaa
     2461 aaatgcgttt gcgggcaggg ggaagcgcgg ggggacacaa tggcctgact tcgattgaag
     2521 aggctttcca aagcgctgaa tatccccgtc ttcgattggg aattgggatc cagcacgaag
     2581 agcatccgga gcgatccgct ggaaccggga cgtatgaacc cgtgcgtgat tatgttcttt
     2641 ctgaattttc ctcggcggag gagaaggata tgacagcctt tctggaaaga ggcgctgaat
     2701 cttgccggcg atgggcccgt ggcccctttg aaaaagccgt tcagtgggtc aattcagcta
     2761 aatcgtagga catctttttg cggttatctt tattgcggaa cccaggggtt tatgctagat
     2821 tgtagctcct ttttggggcc ttccagaagt tttctgcttt ttattgacac aagatatgcg
     2881 gaattctttt cgaggaaaat cggtcgtcta ataggataag aggagttaag tatgaaaaaa
     2941 tacgagggat tgttcatatt tccccctgag gacaaggatc aggcgttgga agtgaccaaa
     3001 tggattgaaa aattccaggg aaaggttctc cagaaagcgg atcttggaaa gaggactatc
     3061 gggtatcaga tcaggaaatt cagggatgga aagttccttg ttttcaattt cgagatggat
     3121 ccttcgaagg tgcaggattt ccgcaaagat cttgagcaac aggaaaccct tttgaaatat
     3181 atggtgacgg ttctccggga agaatccagg ccgagcgttt cgacagcgac ttcccagacg
     3241 actccccaga gttaactttc gaggtgatgt tatggcggcc aatctgaata aagtgatgtt
     3301 gattggaaat cttaccagag atcccgagct tcgttatctt ccctccggcc aggctgttac
     3361 gacgttttcg atcgcttgca accggacgta tgtggcgaaa acgggagaaa agaaggaaga
     3421 agtgagcttt atccggattg tggtgtgggc ccgtcgggct gaggtttgta atgaatatct
     3481 taaaaaagga agcgctgtgt ttgtggaagg tcgacttcag agtcggagtt gggacgcgcc
     3541 ggacgggagc aagcggtctg ccgtcgaagt gattgccgat aatgtgcagt tcctttcccg
     3601 cggcggggga gccaagtctt ctgaccaagc cgacgttccg agcatggatg tggatgatag
     3661 catttttgaa gcgcccgatg cttctggtaa aaagaaccag gcgtcggttt cccaagacga
     3721 acttaaaccg gatgaagaca tcccttttta accaggtgtg aaacatggaa agaaaaccag
     3781 caggaagatt tgagaaaaaa acatttaaga agacggccgg acggcccggc aagccgggac
     3841 gatatggcag ttcggatgat cgcaaggggc ccggtggctc gaaagttttt cgtaagcgcg
     3901 tctgcagggt actccctccg cttaattaca aagactcgga atctttgacg aaatacttga
     3961 cggaaaaagg gaaaattcta ccccggcgta ttacccgact caacgcgaag gatcaacgga
     4021 cgctgaaacg tctgatcaag cgtgcccgcc acgcgggtgt ccttcccttt caggctagct
     4081 aacggagcaa gagacccatg gaactgttac tgatcaagga tgtttttgac gtaggtaaaa
     4141 aaggtgatgt ggttaaagtt cgcgagggat acgggagaaa ttttcttctt ccgaggaatc
     4201 ttgccattcc cgccacgcga aaaaaccagc aattttttga agaacagcgg gcgcgaagcg
     4261 cggcccggaa gttaaaagag aaaacagctg ccgaaaaaaa agcgaaagaa ctggccgagc
     4321 tgacgattac tcttgaggtc aaggcgggtg aaggcgacaa gctttacggc tcggttggcc
     4381 ccgaagaagt ttgcgcggcc cttgtccaaa aagggtacga gtttgacaaa aaacagatcc
     4441 gcatgcggga gcatctgaaa aatctgggca ctcaccatgt ggatctcgag atttacccgc
     4501 aggtgaaaac ccggttgact gtggagcttg tccggcaacc ttaaccctaa gatcctcccg
     4561 tgaggtttgt gtggcgaatt ctgagacatc cgtaaagctt tatgaaaaag tcccgcctca
     4621 aaaccgcgaa gccgaaatga gtgtcctggg cgccatgttt tttgacgaga tggcgcttgc
     4681 caagggcatt gagatacttc gtcccgaata tttttacgat cttcgccatc agcgaatttt
     4741 tgatgtggtt gccggtctct ttgaaagaaa tcaggcggtt gatttgatta ccgcctcgga
     4801 ggagctccgg aagcgtgatc agctggaaga tgtcggggga gtcagctatc tgacccagct
     4861 taccatggcg gttcccacgg cggccaacat tgagtactac gcgaaaatcg tcaaagagaa
     4921 agctcttctc cgccagctca ttcagtccgc tacccagatc gttcaaaaca gcctcgaatc
     4981 cgattcgaat gccaaagaaa tgattgatac ggccgagaaa atgatcttcg atattggcca
     5041 ggcccaggtg gaaggcaagt cattccagat caaggagatc attcatgaaa gcatggaagc
     5101 gatcgaccag ctttttcaga aaaaacagca cgttacgggg ttggcgacgg gatttcatga
     5161 atttgacacc atgaccgctg gtctgcaacc ttcggatctg atcattgtcg cgggtcgtcc
     5221 ctcgatggga aaatccgcgt tcgcgactgc gatcgtggag cacgcgggga ttaatttaaa
     5281 aaaaccggtc gtgatctttt cccttgaaat gagcaaggag caactggtgc agaggatgct
     5341 ctgctcccat gcccgggttg acgcgcagaa ggtgaggacc ggatatcttt ctcaccagga
     5401 ctggcccaag ttgacttcgg cggctggcaa gctttctgaa gccccgatct tcatcgatga
     5461 ttctcccgga caaacggttc tcgaaattcg cgcgaaagcg cggcgcctca agatgaagca
     5521 tgatattcag ttgattgtca tcgactattt acagctcatg cagggcgtgt gcgtggcgga
     5581 aagccgccag caggagattt ccgagatttc gagatctctc aaggctctgg cccgggaaat
     5641 caaggttccc gtgatcgcgg tgagccagtt gtcgcgtgcg gttgaacagc gcaccggaaa
     5701 ccgcccccag ctttcggatc tccgcgaatc aggcgctatt gaacaggacg ctgacctggt
     5761 agtctttctt ttcagggaag aatactatgc tccttccgag gaaaataaaa acaaggcgga
     5821 gtcgatcatt gccaaacaac gcaatggccc cactggctcg gtgcctcttg tttttatcaa
     5881 ggaatggaca cgatttgata atccggagtt ttaccggtcg gatgaggaac agcaatgaaa
     5941 agaatagggt gtcttatcct tgcaatcact ctgatgatga cgagcgctcc tcttcacgga
     6001 tcggaagagc ttgtcgcttc gtcggctgat tccccggcgc ctcaagctcc ggcggaagcg
     6061 aagaagattg cggcgatcga agtccgcggt aataaaattg tcagtaccgc gacgatcctg
     6121 agcaaaattc agtcccagcc cggttctcct ttgcgtcaac agctcacgaa tgaagatatt
     6181 aaacgtcttt atgacacggg gttttttcag gatgtccggt ttgatgtgac ggaaatcgcg
     6241 gacgggtaca agttaattgt tcaggtggac gagaaaccca tcattcgacg gattgagatc
     6301 caggggaaca ccgtttttaa ggaagacaaa ctcaggaaga ccctgggcgt catggaggga
     6361 cagattctgg atcagaaggc ggtgaagaag ggggagacgg aaatccgaca gctttacgcg
     6421 aataaaggtt ttcgttttat cgatattcaa acggaaattg tgaccgatcc gcagacaaga
     6481 gaatcgattg tcacgatgaa gatttttgaa gggaagaagt ttaagataaa agaagttcga
     6541 tttgaaggta atcagtcctt ttcctccaaa caacttgcca agctcatgaa gactcggccc
     6601 cagatgtggg ttttgtttgg tactttcaag gaggaggttt ttgaaaagga tttggaaaga
     6661 attagcttcc actatcagaa agaaggatat ttggacgtta aagtggtccc cgaatttgac
     6721 tacttgaagg aaaaaaacca gatcaatatc ctcatcaaaa tcgaagaggg acagcattac
     6781 gtgaccggcg aggtcaggtt taaggggaac acgcttttcc cggaatcgga gctgtggcag
     6841 gagctggaaa tgctcccggg acttacctat tcccagttct atgtatccca ggacatggaa
     6901 agcatcagga atttttatgc ttccgaaggt tatattgaag cgcgcgttgc tcctgacgtc
     6961 gttctcaata gagaaacagg gaaggttgat gtgacttatg aaatcgcgga gggggaactc
     7021 ttttttgttg aaaaggttca aattcgagga aacaccaaga cgcgcgataa agtcatccgc
     7081 cgcgaattga gaatccgtcc cggggagcgg tttgacgggg ccaaaattga gaaatcaaaa
     7141 cagcgtcttg aaaatctcgg atactttgaa gaaattacct atgataccga acccaccgac
     7201 gtggcgacga atcgccggga tcttattttt cgcgtgaaag aaaaacggac cggggaactt
     7261 tcattcggtg gcggtgtgag ttcgattgac acatttatgg gttttgccga aatttcacag
     7321 cggaattttg atctttttaa ctggccgcga ttgaccggag gaggacaaac cctttcgctc
     7381 agagggcaga tcggaacgat caatcagcag tatgatttta acttcatcga gccgtacctt
     7441 tttgataaac cgatcacgat ggattttaat acctactaca cggttcggga tgattacaac
     7501 gtggatttta aggaaaaacg gtttggtatt agcgaaatat tctcccatct cttcaaggat
     7561 gtttttcgcg tcggaggcgg ctatgtcctt gaacgagtta aactggatga tatcgcgtcg
     7621 gatgcgcccc aggatgtcct tgattttgcg ggtaagaatt ggctgtcgag agttcgaacc
     7681 tttgcggttt atgataaccg tgataacgtg ttcaatccga ccaaaggcag gacggttaat
     7741 gtgggcgccg acctggtggg gagtttcctc gggggggatc aggattacta cattctcagg
     7801 gccgggtata cggaatatat gagtctccgg aaaaatcact tggtggagtt ccaggcccgt
     7861 atcgcgaccg cgcaggattt tggcgattcg acgtcggttc ccgtttttga caggttttac
     7921 gcggggggac tggggacggt acgtggctat gggtatcgcc gggtgagtcc aaaacaaaat
     7981 gataatcccg tgggaggaca aaccatggcg attatcaatc tcgattacag ctttccgata
     8041 cccaagcttg acatgtttcg tggagttgtt tttgcggacg ccggcatggt gaataaaagt
     8101 tcttatgatt tgagttttgg cgatgttgtt gtgagcgtgg gccccggaat taaggtgaag
     8161 actccgatcg ggcctttagc tttttattac ggattcccgc ttgtgaataa agacacagag
     8221 gatgaaaatg gacgttttga atttagctta agtcgcagtt tttaaaccgt tagatcaatc
     8281 aggaggtttt tcatgaaaaa aattgtgtcc gctctgtttt ttgctggttt aatgacatgg
     8341 ttggcgtcgc cggcggtagc tgtagaaatg aagatcgcct atgtggctgt cgacgaggta
     8401 tttgataatt atcagaaaac gaaagatcag gatcttgtgt tgaaaacggc cggtgaagcc
     8461 aaggagagag aacgcaatga gatgatgaac gctttgcgcg cgatggaaga tgaactggct
     8521 cttctttcgg cggattctcg ggcgaaaaaa caagaacagt tgattgagaa aaaacggcag
     8581 atcgaggatt ttgaccggac tgtcagacag caactcgccg aacagcgtga caagacggtc
     8641 cgcgagattt ttgaggaaat tgacagcgtg gttcaggtgg tttgcgaacg cggcggatat
     8701 gaccttgtgt ttaacaagcg agtactcctt tttcaaaaaa gcaactttga tattacgtcg
     8761 caagtgtcgg ccgagttggc gaaacggtac aagaaggcgt aagactgggc agcatgaagc
     8821 aaaggacgtt aaaaaaaagc atccatttcg aagggatcgg ccttcatacg gggcagaagg
     8881 tttcggtaag aattaatccg gcggctgaaa acagcgggat tcttttttcc cgtatggacc
     8941 tggatcccgc ccttaaggtc aaggcttcct tcgaaaatgt tcgttcggac gcgatgcggc
     9001 agacggctct tgcggtcgga agtggtttgg tcaggaccat tgagcattta atggcggcct
     9061 ttcatggcct cggagtggat aacgcctggg ttgaaattga tggcgaagaa gtgcccggaa
     9121 tggatggcag ttcgcaagag ttttcgcgcc tgctccttga agcgggattt gaagagcaag
     9181 gggccgaaag aaaatatctc gaagtcaaga gccccgttta tgtcgaccac ggtgatcagt
     9241 caatcgtgat cgtccctgcc cagagttttc aggtttctta taccctgagc tatcaggatg
     9301 aaaacctcga ggatcagtac atgacgatca cggttctccc ggaaattttt gagcgtgagc
     9361 tcgcgccggc gcggacgttt tgtcttaaaa aagaagcgga gcaacttctc gcgatggggt
     9421 ttgggaaggg agccaatccg ttaaatactc ttatttttga aaacaaccgg ccgatcggca
     9481 atgaactccg gtacgcgaat gaagcatgtc gccacaaaat cgtcgatatt ctcggcgatt
     9541 tgtttctgtg cggcaaattt attcggggac atgtgattgc gcaacgctca ggccattccc
     9601 taaacctgga acttgtgagg aaactagtca tgacgaagga aacgtctctt gatctctctt
     9661 ctaaaaaaac gctaacgatt gacgaaattc aaaaaattat ccctcaccgc tacccgtttc
     9721 tctttgtgga taaaattgaa aattttgagc cgggattgcg cgcgaccgga tggaaacagg
     9781 tgacgatgaa cgattatttt ttccaggggc acttcccggg acacccggtg atgccggggg
     9841 tattgattgt tgaggcgatg gcacaggtgg gaggagtgat catgctcggc gagcctgaaa
     9901 accgggggaa aatagcttat ttcatgagtg tggatcaggt gaagtggcgt aatcctgtca
     9961 gacccgggga ttcccttcgt atggaagtcg aagtattgaa gaaacgatcg aaattcggac
    10021 aatgttcagg caaggcttat gtgggagacc aactggtatg cgaagcggat gtgaaattcg
    10081 ccgtggtgga tgcttgaaaa ttcaccccac cgccattgtc catcccgacg ccattttggg
    10141 cgagggggtt gaaattggac cgtgggcgat agttgaggga acggtcaagg tgggggcgag
    10201 aaccaaggtg ggcccccgtg ttacgattga ggggc
//
LOCUS       DAOSIO010000041         9982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_6549_length_9982_cov_4.079279, whole genome shotgun sequence.
ACCESSION   DAOSIO010000041 DAOSIO010000000
VERSION     DAOSIO010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 9982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9982
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6549_length_9982_cov_4.079279"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PK590_02685"
     CDS             <1..588
                     /locus_tag="PK590_02685"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOG23535.1"
                     /translation="QVRFGFKFVRKDRKPKVGDSIAVSGTCLTVVRGETAGFEVDVVR
                     ETLEATTLGNLKRGDLVNLEKSLKAGDPLGGHFVTGHIDGRGKVIEIERLKKNTTLFV
                     QAPKDIIALQVPKGSIALDGVSLTVQKVHGSVFEIAVIPHTLKVTTLGLKRPGSEVNI
                     EIDIVARYLKAFFPRKRISGWSSKNILKMILRKGF"
     gene            592..996
                     /locus_tag="PK590_02690"
     CDS             592..996
                     /locus_tag="PK590_02690"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG23536.1"
                     /translation="MEKVFELESNAEILIDLRKELRDLLRQAGWDGKSMDKIVLSVDE
                     ALTNVIRHAYAGVRGPMRLTFRDSEEKTEITIEDHGKKFDPTLVPEPKLPREEPGGLG
                     VHLIRTIMDELIYDKNFLEGNRLRMIKRKPRK"
     gene            1013..1357
                     /locus_tag="PK590_02695"
     CDS             1013..1357
                     /locus_tag="PK590_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG23537.1"
                     /translation="MKIQMTNQGDVSVVELEGELDFHSSPELRSRLGELASKQAPKIL
                     IDLKKVGYMDSSGIATFVEAFQKTKRYHGRLILADLTPAVRGIFEIAKLESIFEIATD
                     QDEAMNRFAGKN"
     gene            1375..2175
                     /locus_tag="PK590_02700"
     CDS             1375..2175
                     /locus_tag="PK590_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG23538.1"
                     /translation="MKEICGKVGRSTFLVLAGVARVWKLGTQVGRYSLTGFIRPKTIP
                     RETFFRQMVLMGIDSTAIVSLVGASVGTVLALQAAYSLKAFGAIIYTGSLVGVAMARE
                     LGPLIAAIVICGRIGARMAAELGTMMVQEEVDALTTMAINPVRFLVLPRVLALAIMLP
                     CLTVIADLMGMLGGYLIGITGLGINSQVYISKTIDALVLKDIYTGLAKSFIFSILIAL
                     VSCHQGLSVSGGADAVGKATTQAVVISIVLIIVVDCLATAVIYYALPT"
     gene            2203..2988
                     /locus_tag="PK590_02705"
     CDS             2203..2988
                     /locus_tag="PK590_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG23539.1"
                     /translation="MIKIKNLVKRFGDRAVLDGIDIAIPEGKITVIMGGSGCGKSTLL
                     RHLIGLHQPTSGEVWVDGRNIAGLDEKGMNEVRKKFGMLFQGSALFNSMTVGQNVAVP
                     LKEHTDLSPEIIKIIVRVKLELVGLTGFENFMPHEISGGMKKRVALARAIALDPKIVF
                     YDEPGAGLDPITASMIDRLILDLSKKLSITSVVVTHEMKSAFRIADQIVILQKGKVIQ
                     VGTAEEIQNSKNAYVQQFIQGESEGAIPFKQTSEAYLKGLLDT"
     gene            3004..3636
                     /locus_tag="PK590_02710"
     CDS             3004..3636
                     /locus_tag="PK590_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOG23540.1"
                     /translation="MASSSNYSSTEIRSGFFVILSILILLVMTFVVGGIFKGGSEEWK
                     VRFGYLNGLEDSAPVYYAGREVGKVSKIEIVRGAPRPILVTVKVSPDAYVRTDSQAYI
                     DTLGMMGEKFVEISPGSPDAKALEKGSVIEGHDPIAMHVMVGKMNRLADQMDVMVKSL
                     NPLVESASSILEGHEEEISKSITNINQITANLRDMTDDLKHRPWRLLRKG"
     gene            3640..3987
                     /locus_tag="PK590_02715"
     CDS             3640..3987
                     /locus_tag="PK590_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase system-associated protein, TIGR04073
                     family"
                     /protein_id="HOG23541.1"
                     /translation="MYFSRILGLFLAAMLLCIPGSFAQETAGDYFYGIGTKLGRGLEN
                     VVTSPVEIPCTMADDIKDQGALAGSFTGFGKGTLFMLRRILVGVTEVGTFFIPMERTL
                     PRVCRKEKATIAV"
     gene            3994..4278
                     /locus_tag="PK590_02720"
     CDS             3994..4278
                     /locus_tag="PK590_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23542.1"
                     /translation="MKKPTFFILSCTRARKRFDEEFETVVFDKIYQKLKSKIRQGCSL
                     FILGGSPGRKDAGMVAEVVAKLKIQHPRVDFHFAGSMRSGRRMFGIDASC"
     gene            4323..4664
                     /locus_tag="PK590_02725"
     CDS             4323..4664
                     /locus_tag="PK590_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23543.1"
                     /translation="MTTSNSQNPAKDPEIQDAKFFAAIGYVSFLCFVPLALKKKNSFA
                     EFHGKQGLVLFILELAAAILSVIPIIGDLIAQLALVVFGIFSLIGIVKVLMGEKWEIP
                     VIHEIATKITL"
     gene            4708..6249
                     /locus_tag="PK590_02730"
     CDS             4708..6249
                     /locus_tag="PK590_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23544.1"
                     /translation="MVNRKPQLTGQNWFIVLNPLKSELERSKIAREIAKAFRIPADEA
                     IDLVANTPIILLDNLNYSTAIQVKQYFCASVSDLIVSNDGALKRRCYRTVWPNPPSLT
                     FLKDMDAKSLPRIEEEKLAEDEAIQEIRSWIQTENGFSEKETASSGETGHASRHFRDM
                     LEEERERLLKENVGLRDNLNQIHRALEKIQSELHLKEKALENIQNEKKQKERETQEMQ
                     TLLNHSEEKYEMLREEFRQTRQYFEEKLVTNEKKSEELMKQIEEREIANRELMEEKQE
                     IQKSFHASKNDLLIARDEKERSEKEWKERTAALFRELETQKKMVAEINDKLNTMEEGK
                     KIIQSSEQRLLKEVEAQMHQTKRWELKSSELESEMGRMREAFEGQIKSWQLRLAQLES
                     REHELEKARAQVKDLQRQLEHRELIQRKRELSESYSMKETQLKSLVKQQEKLESEIRH
                     REEELRKLLHEQGTLEREVIEIKQAQRHLAERVKRESPGSVPRSQDTSTDFFLPRESP
                     ETSHD"
     gene            6242..6646
                     /locus_tag="PK590_02735"
     CDS             6242..6646
                     /locus_tag="PK590_02735"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOG23545.1"
                     /translation="MIELVLSKIKVDEKRHEQLLVFKVQGETRYLPLVIGIAEINSIK
                     LNLSGVKPPRPMTHDLLVNILEGLGARLEKVVIDKIEKNTFYAKLFILKEGGNRIIMD
                     ARPSDSVAIALRCGVPIFAKQDVLNAASVAEV"
     gene            6652..7578
                     /gene="trxB"
                     /locus_tag="PK590_02740"
     CDS             6652..7578
                     /gene="trxB"
                     /locus_tag="PK590_02740"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOG23546.1"
                     /translation="MSTSLFDTVIVGAGGAGLTAAIYTARANLSTLVLEKLMPGGQIA
                     STEGVENYPGFSEGIMGADIAQRMEAQAVKYGVQINYEDVKNVLPREKFFLIETSENR
                     YQAKAVILAMGASFRMLGVPGEKEFIGRGVSYCATCDGAFFKGKNVMVVGGGDSALQE
                     GIFLTKFASQVSIVHRRDQLRGSSILQERAKKNPKIRFIWDTVIDKIQGNSKVESVLL
                     RNVKTQETRTMPADGVFVFIGHAPNSGIVKGLVEMDEEGYVISGGGYRTSVPGIFAAG
                     EIRKGSVHQLVAAAGEGCGAALALQAYLENQE"
     gene            7601..7909
                     /locus_tag="PK590_02745"
     CDS             7601..7909
                     /locus_tag="PK590_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23547.1"
                     /translation="MSSEREFENHMNQLLQLLKKIMVQYPHHGKIEDLAKHMKNAKSS
                     PEINIFFLNLVPLTPEEFDELEEMFEETLSQDRWQHGELQYELNADDREFLKKHGIRF
                     "
     gene            7918..8778
                     /locus_tag="PK590_02750"
     CDS             7918..8778
                     /locus_tag="PK590_02750"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HOG23548.1"
                     /translation="MKRITILFIFFIFCFQTLSVKAAGDSLDGHAVWRSFLHQFVNEN
                     GDVNYAGVKRNPALLNDYLAFIAAQNPVDVKTLWPREKAMAFWINAYHAGVIKIVVDH
                     YPARSLNQISSAWDITVIHVGAEQYSLNQIRNQILMKTYRDEKIHLALSCGARGCPRL
                     DRDPFTDANVEGKLFLATREFLNDPARVEIIPGKKTIRLSRIFKWYGADFNLDFGTPE
                     RIGKFSREEMSILSFLIYYSEDLQKIEFLEEGRYKIKYMPFDPELNDWIDAPPVSDHS
                     LKYELPGEPK"
     gene            8908..9228
                     /gene="rplU"
                     /locus_tag="PK590_02755"
     CDS             8908..9228
                     /gene="rplU"
                     /locus_tag="PK590_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOG23549.1"
                     /translation="MSAYAIVETGSKQYWVEPKSILEVELLEGTGGQGEVVLDRVLFY
                     ADGNTVLVGTPVVKGAQVICENLGEIRAPKVIHYRMRRRKASRRKHGHRQDLLRLRVK
                     DIKV"
     gene            9253..9495
                     /gene="rpmA"
                     /locus_tag="PK590_02760"
     CDS             9253..9495
                     /gene="rpmA"
                     /locus_tag="PK590_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOG23550.1"
                     /translation="MAHTKSQGSSTNGRDSQSQRLGVKVFGGQKVRAGSIIIRQRGTR
                     FHPGQFVGIGNDHTLFAMRDGIVEFKSNRTVHIIPS"
     gene            9571..9789
                     /locus_tag="PK590_02765"
     CDS             9571..9789
                     /locus_tag="PK590_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23551.1"
                     /translation="MLIDKIHLTVQGGHGGHGCDSYYHRIDRKIVPNGADGGDGGSVI
                     FRASHNAPGLDRLKLKQYLGTRGHSRWA"
BASE COUNT         2627 a         2029 c         2747 g         2579 t
ORIGIN      
        1 caggttcgat tcggatttaa gtttgtccgc aaagaccgga aaccgaaagt tggagacagt
       61 atcgcggtta gtggcacctg cctgactgtt gttcgcggcg aaacagctgg tttcgaggtg
      121 gatgttgtgc gggaaactct ggaagcgact acgcttggaa atttgaaacg cggggatctc
      181 gtcaatctcg aaaaatcgtt aaaagcagga gaccctctgg gcggacattt tgtgacggga
      241 cacatcgatg gccgggggaa agtcattgaa attgagcgac tgaagaagaa taccaccttg
      301 ttcgttcagg cccctaagga tataatcgcg ctccaggtac ccaaaggatc tatcgcgctt
      361 gatggggtca gtcttacggt tcagaaggtt catggttccg tttttgaaat cgcggtgatt
      421 ccccatacgc ttaaagtgac aaccctgggg cttaaacgtc cggggtctga agtgaatatt
      481 gagattgata ttgttgctcg atatctgaag gctttctttc ctaggaagcg gatttcaggg
      541 tggtcatcga aaaacatctt gaaaatgatt cttcgaaagg gtttttaaaa attggagaaa
      601 gtttttgagc tggaatcaaa cgccgagatt ttgatcgatt tacgtaagga attgcgagat
      661 ttgctcaggc aggccggttg ggacggaaag tccatggaca aaatcgtgct ttccgttgat
      721 gaggcgctga ccaatgtgat tcgtcacgcg tatgccggcg ttcgcggccc gatgcgactg
      781 accttccgtg attcagagga aaaaaccgaa atcacaattg aggaccacgg gaaaaaattt
      841 gatccgacgc ttgtccccga gccgaaactc ccgcgggaag agccgggagg tcttggcgtg
      901 catttgatcc gtacgatcat ggatgagctg atttacgata agaatttttt ggaaggtaac
      961 cgattacgca tgatcaaacg aaaacctcgg aaatgaaagc gaaaggtgat ttatgaagat
     1021 tcaaatgaca aatcagggag atgtaagcgt ggttgaactg gaaggggaac ttgatttcca
     1081 ctcctcacct gaacttagat cgagactcgg ggaactggca tccaaacagg cgcccaaaat
     1141 actgatcgat ctgaaaaaag tcggatatat ggacagctcc gggattgcca cgtttgtcga
     1201 ggcttttcaa aaaaccaaac gatatcacgg gcggcttatt ctggctgatt tgacgccggc
     1261 tgtccggggt atttttgaaa tcgcgaaact ggaaagtatt tttgagattg cgacggacca
     1321 ggatgaagcg atgaaccggt tcgcgggaaa aaactgaaga ggatatttat tctcatgaaa
     1381 gaaatttgtg gcaaggttgg gcggtcgacg tttcttgttt tggcgggtgt cgccagggta
     1441 tggaagctgg gtactcaggt gggacgctat tccctcacgg gatttatccg tcctaaaacc
     1501 atcccgcgcg aaacgttttt tcggcaaatg gtattgatgg gaattgactc aacggcgatt
     1561 gtttcgttgg taggggcgtc ggtcggcacg gttcttgcgc tgcaagcggc ttattcactc
     1621 aaggctttcg gggcgatcat ttatactggt agccttgtgg gtgttgcgat ggctcgcgaa
     1681 ttgggcccgc tgattgcggc gatcgtaatc tgcggacgta ttggcgctcg tatggctgcg
     1741 gaactgggta ccatgatggt tcaagaagag gtggatgccc ttaccaccat ggcgattaat
     1801 ccggtgcgtt ttcttgtatt accgcgagtt ttggctctgg ctatcatgct cccttgtctt
     1861 acggtcattg cggacctcat gggaatgcta ggagggtact tgatcgggat tacgggcttg
     1921 ggaatcaact cccaggttta tatcagcaag acgattgatg cgttggtttt gaaagacatt
     1981 tatacgggac tcgccaaaag ttttattttt agtatcttga ttgccctggt cagttgccat
     2041 cagggattat cggtgtcggg gggagcggat gctgttggaa aggcaacaac gcaagccgtg
     2101 gttatttcca ttgtccttat tattgtggtc gactgtttgg caacggcggt tatttattac
     2161 gccctaccaa cttgatgcga gaggttaaaa gtggccgatc ctgtgattaa aattaaaaat
     2221 ttggtgaagc gatttggtga tcgtgcggtt ttggatggta ttgatattgc gattcctgaa
     2281 gggaagatca cggtcattat gggaggatcc ggttgcggca aaagcacgct tctccggcat
     2341 ctgatcgggt tgcatcaacc aacttcaggt gaggtctggg tggatggcag gaatatcgcg
     2401 ggacttgatg aaaagggaat gaatgaagtt cgaaaaaaat tcggtatgct gtttcagggg
     2461 tccgctcttt ttaattcgat gacggttggg caaaacgtgg cggtccccct caaggagcat
     2521 acggatctgt ctcccgagat catcaagatt atcgtgaggg taaagctgga gctcgtcggg
     2581 ctgaccggtt ttgagaattt catgccccac gaaatttccg ggggtatgaa aaagagagtg
     2641 gcgctcgcgc gtgccatcgc gcttgacccc aagatcgtgt tttatgatga accgggcgcg
     2701 ggactggacc cgatcacggc gagcatgatt gatcgcctga tccttgacct ttccaagaaa
     2761 cttagtatca cttcggtggt ggtgacgcat gaaatgaaaa gcgcttttcg gatcgccgac
     2821 cagattgtga ttctccagaa ggggaaagta atccaggttg gaaccgcgga agaaatccag
     2881 aattctaaaa atgcttatgt ccagcagttt attcaagggg aatccgaggg cgcgattccg
     2941 ttcaaacaaa caagcgaagc ttatctaaaa gggttgctcg acacgtaatt taaaggagga
     3001 aatatggctt cttcatcgaa ttattcatcc acggaaatcc ggtctggttt tttcgtgatt
     3061 ctctcaattt tgattttgct ggtcatgaca tttgtggttg gtggaatctt caaagggggt
     3121 tctgaagaat ggaaggttcg tttcggttat ctgaacgggc tggaagacag tgcgcctgtt
     3181 tattacgccg gtcgcgaagt cggaaaagtc tctaaaatcg aaattgtccg tggcgctccc
     3241 aggcccattc ttgtcacggt caaggtttct ccggacgcct atgtgcggac cgatagccag
     3301 gcttatatcg ataccctcgg gatgatggga gagaagtttg tcgaaatatc tcccggttct
     3361 cccgatgcca aggcgttgga aaaggggagt gtgattgaag gacatgaccc gatcgccatg
     3421 catgtgatgg ttggtaaaat gaaccggctg gcggatcaga tggatgtgat ggtgaagagt
     3481 ttgaatccgc tggttgagag tgccagttct attttggaag gtcatgaaga agaaatttca
     3541 aaaagcatta ccaacatcaa ccagattacc gccaatcttc gggacatgac ggacgatctg
     3601 aaacatcggc cgtggcgtct tctccggaag ggataaaaaa tgtatttttc aagaatattg
     3661 ggtctgttcc tcgcggcaat gcttttatgt attcctggat ctttcgcgca ggaaacagcg
     3721 ggagattatt tctacggtat cggaaccaag ttgggccggg gtctcgaaaa tgtggtgact
     3781 agtcccgtgg aaattccttg taccatggcg gatgacatca aggatcaagg agctctggcc
     3841 ggatcgttca cgggatttgg caaagggact ttgtttatgt tgaggcggat attggtcggc
     3901 gtgacggaag tcgggacatt ttttattccg atggagcgga ctctcccaag ggtttgccgg
     3961 aaagaaaaag caacaattgc cgtttagtca gcgatgaaaa aaccgacttt ttttattttg
     4021 tcctgcaccc gggcgagaaa acgatttgat gaagagtttg aaacggttgt tttcgataag
     4081 atttatcaga aattaaaaag caagatacgt cagggatgtt cattgtttat tttgggaggt
     4141 tcacccgggc ggaaggatgc ggggatggtc gcggaagtgg tcgcgaaact caaaattcag
     4201 catcctaggg tagactttca ttttgccggt tctatgaggt cgggtcgaag gatgtttggc
     4261 attgacgctt cttgctgaag atacctataa taaacaaact ttttgatctc taaggagaat
     4321 ttatgacgac gagtaattca cagaacccgg caaaagatcc agaaattcaa gacgcgaaat
     4381 tttttgccgc catcgggtac gtcagttttc tttgttttgt tcctctcgct ctgaaaaaaa
     4441 agaattcctt cgcggaattt catggaaagc aggggctggt tctctttatt ttggagcttg
     4501 cggcggccat tctctcggtg atcccgatta tcggcgatct tatcgcgcag ttggcgctag
     4561 tggtatttgg cattttttcg ctcatcggta ttgtcaaggt tttaatgggg gaaaagtggg
     4621 agattccggt gattcacgaa attgcgacca aaataacact ctaggctgat gtgatcatcc
     4681 acttttttaa agggcaaaat ttccaccatg gttaatcgta agcctcaact aaccggacaa
     4741 aactggttca ttgttcttaa tccgctgaaa agtgagctgg aacggagcaa gattgcgcgt
     4801 gaaatcgcga aagcgttccg tatccctgcg gatgaggcca ttgatctggt ggcaaacact
     4861 ccgatcattc ttctcgataa tctaaattat tcgaccgcca ttcaggtcaa acagtatttt
     4921 tgcgcttccg tttcggatct gattgtcagc aacgacggag ctttgaaacg tcgctgttac
     4981 cggacggttt ggccgaatcc gcccagcctg actttcctca aagatatgga tgcgaaaagt
     5041 cttccgcgga ttgaggagga aaaactggcg gaagatgagg cgatccagga gattcgttcc
     5101 tggattcaga cggaaaatgg tttttctgaa aaagaaacgg cgtccagcgg ggaaacgggc
     5161 cacgcctcgc gtcatttccg ggacatgctc gaagaggaac gcgaacgtct cctgaaggaa
     5221 aacgtcgggc ttcgtgataa tcttaaccaa atccaccggg ctcttgagaa aatccagtcg
     5281 gaacttcact tgaaagaaaa agcactggaa aacattcaga atgaaaaaaa acaaaaggag
     5341 cgggaaacac aggagatgca aaccctcctg aaccactccg aagaaaagta tgagatgctt
     5401 cgggaggagt tccgtcagac gaggcaatac tttgaagaaa aactcgtgac gaatgaaaaa
     5461 aaatccgaag agcttatgaa gcagatcgag gagcgtgaaa tcgcgaaccg cgaattgatg
     5521 gaagaaaaac aggagattca gaaaagcttt cacgcttcca agaatgatct tttgatcgcc
     5581 agggatgaaa aggaacggag tgagaaggaa tggaaagaac gaacggccgc cctgtttcgc
     5641 gagctggaaa cgcaaaagaa aatggtggct gaaatcaacg ataagctgaa caccatggag
     5701 gaaggaaaaa agatcattca gtccagcgag caaaggttgc ttaaagaggt tgaggcgcaa
     5761 atgcatcaga cgaagcggtg ggaactcaag tcttccgagc tggaatcaga gatggggaga
     5821 atgcgtgagg cttttgaggg acagatcaaa tcctggcaac tccgtttagc gcagcttgaa
     5881 tcccgggagc acgagcttga aaaagcccgt gcccaggtta aggatttgca gcgacaactg
     5941 gagcatcgcg aactgattca gcgaaagagg gaactttcag agtcttacag catgaaagaa
     6001 acacagttga aaagcctcgt taaacagcaa gaaaagctgg agagtgaaat ccggcatcga
     6061 gaagaggagc ttagaaaact tctccatgaa caaggtactc ttgagcggga agtcattgaa
     6121 attaaacagg ctcaaaggca tcttgcggaa cgtgtcaaga gggaaagtcc cggttctgtc
     6181 ccgcgtagtc aggatacttc gactgatttt ttcttgcctc gtgagtcgcc cgagacgtct
     6241 catgattgaa cttgttttaa gcaagatcaa ggtggatgaa aagcggcatg agcaattgct
     6301 agtttttaag gtccagggtg agacgcgata tctgccgctt gtgatcggaa ttgctgaaat
     6361 caattcaatc aagctcaatc tgagtggcgt caaaccccct cggcccatga cccatgatct
     6421 tcttgttaat attctggaag gactgggcgc tcgtctcgaa aaggtggtca tcgataaaat
     6481 cgagaaaaac actttttatg caaaattatt tatcctcaag gaaggcggga atcggatcat
     6541 catggacgcc aggccctcgg acagtgtcgc aatagcactg cgttgtggag ttccgatttt
     6601 cgcgaaacaa gatgttctga acgcggcgag cgttgctgag gtttgaaacc gatgagtact
     6661 tctttgtttg atactgtgat tgttggggcg ggtggagcgg gtctcaccgc ggcgatttat
     6721 acggcgcgag cgaatctctc cacgcttgta ctcgaaaaac tgatgccggg tgggcagatt
     6781 gcttctaccg agggtgttga aaattatccg gggttttccg agggcattat gggggctgat
     6841 atcgcgcaac gcatggaggc ccaggccgta aaatacggcg ttcaaattaa ttatgaagac
     6901 gtaaaaaatg ttcttccccg ggaaaagttt tttttgatag aaacgtcaga aaaccgttat
     6961 caagcgaagg cggttatcct tgccatgggt gcatcattcc gaatgcttgg tgtacctggg
     7021 gaaaaggagt ttatcggccg aggagtttcc tattgcgcca cgtgcgacgg agcttttttt
     7081 aaaggaaaaa acgtgatggt ggtgggtggc ggagattcgg ctttgcagga aggtattttt
     7141 ctgacgaaat ttgccagtca agtctctatt gttcaccgac gtgatcaact tcgcggaagt
     7201 tccatcctcc aggagcgagc aaaaaaaaat cccaagatac gctttatttg ggatacggtt
     7261 attgataaaa tccagggaaa ctcaaaagtg gaatcggttt tgctccgcaa tgtgaagact
     7321 caggagacac gaacgatgcc tgcggacggc gtttttgttt ttattgggca cgcgcccaac
     7381 tcggggattg tcaaggggct tgtcgaaatg gatgaagaag gctatgtgat ctcgggtggg
     7441 ggataccgga catccgtccc aggaatattc gcggcaggtg aaattcgcaa aggatcagtg
     7501 catcagcttg tggcggcggc cggagaaggc tgtggcgccg cgttagccct tcaggcttat
     7561 cttgaaaacc aagaataagg ccgttttaaa ggagattttt atgagttctg agcgtgagtt
     7621 tgagaatcac atgaaccagc ttttgcagct tttaaaaaaa ataatggtac agtatccgca
     7681 tcacgggaag atcgaagatc ttgccaaaca catgaaaaat gccaaaagct ctcccgagat
     7741 taatattttt ttcctgaatc ttgtcccctt gaccccggag gagtttgacg agcttgaaga
     7801 aatgtttgag gaaactcttt cccaggatcg ttggcagcac ggagaactcc agtatgaatt
     7861 gaatgccgat gatcgggaat ttctgaaaaa gcacggtata cgattctgat gactttcatg
     7921 aaacgaatca cgatactttt catatttttt atcttctgct ttcagacgct ttcggtcaag
     7981 gcggccgggg attcgcttga cggtcatgct gtctggcggt cgttccttca tcaattcgtg
     8041 aacgaaaatg gtgatgtcaa ttatgccggg gtaaagcgta atccggctct tttaaatgat
     8101 tatctggcct ttatcgcggc gcagaacccc gtggatgtca aaacgctctg gccacgtgaa
     8161 aaggcgatgg cgttctggat taatgcttat cacgcgggcg tgatcaagat tgtggtggac
     8221 cattatccgg cacgcagtct taatcagatt tcaagcgcgt gggatatcac tgtgattcac
     8281 gttggggcgg aacagtacag tttgaatcag atcagaaatc agattctgat gaagacttat
     8341 cgtgatgaaa aaatacatct tgccctttcc tgtggcgcgc gggggtgccc ccggttggat
     8401 cgtgatcctt ttacggatgc caatgttgaa ggaaaacttt ttctcgcgac ccgcgaattt
     8461 ttaaacgatc cggcccgggt tgaaattata ccgggcaaga agacaatcag gctttcccgt
     8521 atatttaaat ggtatggagc ggattttaat cttgatttcg gaactcctga aaggatcggg
     8581 aagttttctc gggaggagat gtcgattctc tcattcctta tctactattc agaggatctt
     8641 caaaaaatcg agtttcttga agagggccgt tataaaataa agtacatgcc gtttgatccc
     8701 gagctcaatg actggatcga tgcgcccccg gtgtcagatc attctctgaa gtacgaactt
     8761 cccggcgaac caaagtaaat agaacgttca cgccgaaaac gttgtaactt gttatggctg
     8821 aacaagttat aggtttttta attttgttga cacagtggtt ctatttccat agaataccca
     8881 ttctctttaa gtcaaaagga gttatttatg tctgcgtacg caatcgtgga aacgggctca
     8941 aagcagtact gggtggaacc taagtcaata ctggaagtag agttgctaga ggggaccgga
     9001 gggcaaggcg aggttgtgct tgatcgtgtc cttttttatg cggatggtaa tacggtattg
     9061 gtggggacgc ctgtggtgaa gggggctcaa gttatttgtg aaaatcttgg cgaaatacgc
     9121 gcgcccaaag ttattcatta ccgtatgcgg cgtcgcaagg cgtcccgccg caagcatggt
     9181 caccgccagg atcttctgcg attgcgtgtt aaggatatca aggtttgatt taaattattt
     9241 ttagaggaca tcatggcaca tacaaaaagt cagggaagca gtactaacgg gcgagattca
     9301 caatcccaac gtctgggcgt caaggttttt ggcgggcaaa aggttcgcgc gggcagtatc
     9361 attatccggc agcgtggaac ccggtttcat ccggggcagt ttgtgggcat tggaaacgac
     9421 cacaccttgt tcgcgatgcg tgatgggata gtggaattta aatctaatag aacagtccat
     9481 attattccca gctagatatt tattatcttt catgaccaag gctaaaaggc gctggcgcgc
     9541 tttttggcct ttttcttttt aaggagtttt atgttgattg acaagattca tttgaccgtc
     9601 cagggtggac atggtggaca tggttgcgac agctattatc atcgaattga ccgaaaaatt
     9661 gtcccgaatg gcgcggatgg tggcgacggt gggagcgtca tttttcgggc ctctcataac
     9721 gctccggggt tagaccggct taaactcaaa cagtatcttg gcacccgcgg gcactcacgg
     9781 tgggcctaat aacaagcggg gagcttgtgg cgaggatctc gtggttctcg ttcctgtcgg
     9841 aacttcaatt tacaacaagg aaaaccgcct cgcgattcgc gacctaaacc aggatcaaga
     9901 tgaagtcgta gtcgctcgtg gcgggcgtgg cggatccggc aacattggag ataaaaaagc
     9961 gacgcctggt caggaaggtg aa
//
LOCUS       DAOSIO010000042         9810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_6686_length_9810_cov_4.395592, whole genome shotgun sequence.
ACCESSION   DAOSIO010000042 DAOSIO010000000
VERSION     DAOSIO010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 9810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9810
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6686_length_9810_cov_4.395592"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="PK590_02770"
     CDS             <1..1103
                     /locus_tag="PK590_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG23552.1"
                     /translation="PDLMLIGTAEPLALDMKNLEKAYAIPEVRNDLIAHGIKDPAGLL
                     SAAWLFDPSLRRLARGAKINSDNHPYLEFSAPRSLYKDTVEDNFRLLSPFYRLDSFPL
                     IRNLEPPQDKNPAFYNSLARGYLVKSFYANAENALSVSEKIDPAHPETRLLRGIFYYL
                     LGMKERAREILIQVVITSPDWAEAYEYLGLISQDSGKFTEAVNSFRKACELEPENIDH
                     QIRLANALLKAGETKEAIEAFDNILTRRPEDFENRVKRAELIVKTGSLEQGLSAAHDV
                     IKKYPRFMGAYEWVGSILEKNGQLDQALAVYDNMLSLFPNDPTPHINLARLSESLGRP
                     QDMKWHLEKAAALNPALTKVPSVRKLLASDPR"
     gene            complement(1603..3009)
                     /gene="lpdA"
                     /locus_tag="PK590_02775"
     CDS             complement(1603..3009)
                     /gene="lpdA"
                     /locus_tag="PK590_02775"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOG23553.1"
                     /translation="MENLKIDVVVIGGGPGGYASASYAVSCGKKVILIEKNERLGGTC
                     LNCGCIPSKALLHVAEAVYGARAAEKLGLGFSEPSLDLAKLTAWKSEMIEKLGRGMKD
                     LGGRRGIEIIQGRATFLDQRTVKVADKRITFEHAIIATGSTPALPKSLKISSPLVMTS
                     TEALQLQEIPKRLLVIGAGYIGMELGTVYAALGSKVTVVEVLPRILNGADSDLIRYVE
                     EMAKRRLEKLMLNTKIAGLENCQNQVKAVIDAGGNITEELFDRVLVSTGRAPLTAEVR
                     LEEIGVRLDERGFVRTDSAKRTSVPSIYAVGDVTGGVMLAHKASCDARLAIDSIMGFP
                     TPKSFPLIPAVVFTDPEVAWCGVTENEARSAGTPVQVIRFPWTASGRAVTMDRTDGVT
                     KLLIDPATEKIIGAGICGKGAGELIAETVLAIEGEMTAKELGRRVHPHPTLSETVVEC
                     AEMFYGRSTSAFSRKRNG"
     gene            complement(3150..4061)
                     /locus_tag="PK590_02780"
     CDS             complement(3150..4061)
                     /locus_tag="PK590_02780"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOG23554.1"
                     /translation="MMTESVSRPPANLSEFSIIARNASRQVKDQIQRFFEDAGIPSAG
                     LVLWNYKKYFRVSAYALTTKQRQRITKAAKCSRLRGLCVQGKWLRPKDWFYKWQESYR
                     KISVGKKFLIVPAWKKIGKTGGKRIPVVLDPRSAFGTGGHETTRLMIRMMESLEGKFT
                     RFLDVGTGSGVLSVIASRLGAEEIHGFDYDAMSARIARENFVRNGCQGGRFFRANLGK
                     VSGTGRFDLVGANMLSRTLLRYRKKLASLTCPGGFLVVSGISKRHFPDFRKQFPTDHL
                     RLIRVLNGRSWSAALYRRNQNRPSYRG"
     gene            complement(4064..4915)
                     /locus_tag="PK590_02785"
     CDS             complement(4064..4915)
                     /locus_tag="PK590_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOG23555.1"
                     /translation="MNFIQKNGNDKTMLSARIASRLRVIVIGAGISALVAGSAFASDV
                     KEPDDYRLLKEIILIEFADEKPHEAGPVVPGVMTQIDSQENIMALTFNINANESVPLD
                     TRLLDFLRKENIPAVFFVSGKWIEKNPEWIKRIGSDELFEIGSLGLEGKACTVTGENV
                     PRELSAARSVEELFIEIEKNARKIESLTGALPRFIRSPSSYYDDVAVRMARAMGYEVI
                     GSMMPDPHSENPPKPEEIFPARSGAIVAMTLEDSGIQAAIALRKTVPRLKSKGFRFVK
                     LGDCSLR"
     gene            complement(4912..5469)
                     /locus_tag="PK590_02790"
     CDS             complement(4912..5469)
                     /locus_tag="PK590_02790"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HOG23556.1"
                     /translation="MIIYLASQSARRKEILRRMKIRFRVVPSSYQERFIPGRAPRDLV
                     LKHAEGKAIHAKVPRIARYVLAADTLVWRKKIYGKPKSLPEARKMLRSLSGKVHEVYT
                     GVVLWDRVRKRLMKGVSCTRVQFKKLTSEEIGDYLLAIRPLDKAGAYAIQEGPRIVER
                     IQGSYSNVVGLPRELVRRLLRKISG"
     gene            complement(5473..7272)
                     /gene="treZ"
                     /locus_tag="PK590_02795"
     CDS             complement(5473..7272)
                     /gene="treZ"
                     /locus_tag="PK590_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HOG23557.1"
                     /translation="MGGGYPVCGANLEKDGTVSFVVWAPRCEKIELHLINHDGSRLVE
                     MRKDPLGYFRVRASGVRPGDRYFFRLNGTEDRPDPASRYQPEGVHGPSCVVDSAAFSW
                     TDQAWKGIAFRDLIFYELHIGTFTPEGTFESAIRKLPYLKRLGITCVEIMPIAQFPGS
                     RNWGYDGVGLYAAQNSYGGPEGFKKFVDACHREELAVCLDVVYNHLGPEGNYLHGYGP
                     YFTKKYRTPWGDALNYDDQDCEPVRQFVIENALYWVSEYHVDSLRLDAIHGIFDSSPT
                     HILRDLNERVESLARVLGREVHVIAESDLNDSRVIRPYHEKGYGLAGQWSDDFHHAVH
                     GCLTGERNGYYSDFSGVADIAKALSDGFVYDGRYSAFRQKIHGNPVKDLDPRKLVVCV
                     QNHDQVGNRAWGDRLSTLTDVEREKIAAVLLILSPNTPLLFMGQEYGEKAPFQYFIDH
                     GDPDLVRAVREGRTREFASFGWKEIPAPESEKTFFDSRLTWKDKEGTHAEIWHLYQTL
                     MTLRKERLGRSRLSGVYFDEEKQWLAFEYDEGMKVLVSFLDRPQEIVLPFHAGVSREI
                     LNTRRWGTCGRVSEIGKDGKTLCLPSRCAWVGE"
     gene            complement(7277..>9810)
                     /locus_tag="PK590_02800"
     CDS             complement(7277..>9810)
                     /locus_tag="PK590_02800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HOG23558.1"
                     /translation="FPIMPRLFMAIHMEDRFPIVDILQQTPEIPDSCQWAIFLRNHDE
                     LTLEMVTDEERDYMYRVYAHDPKARINLGIRRRLAPLLGNHRRKIELMNGLLFSLPGT
                     PVIYYGDELGMGDNIHLGDRNGVRTPMQWSADRNAGFSRANPQKLYLPVIIDPEYDYG
                     TVNVEAQQGNTHSLLWWMKRLIALRKRYKAFSRGSIEFLSPENGKVLVFIRKYREEQI
                     LVIANLSRFVQCVEIDLSAYKGMVPVEMFGHNEFPSIGELPYFVTLGPHSFYWFSLET
                     APKAQPALISSPPTELKKIVVKDHWTRMFKGDAKTHLESLLPDYIRACRWFAGKARKL
                     KRVQITEAVTVPCNAGDSFLVFLKVEYAEGEAEYYLLPVAYASGSRAGEIQTQFPLSR
                     IAQVEVKGSGGITDGVLYEAIVDRTFTSAILDGIHRRRRFKGAQGVIQGTPTAELKKL
                     WPKDSSLRESSLMKAEQSNTSINYGGRMVLKLFRKIEEGINPDFEMGRYLTENTDFVN
                     VPRMLGSLEYSRGRSSYATIGVLQEFIKNQGDAWQYTLDALASYFDGVFARPDKFKKR
                     QSCEKSLMELAQGEVDSLATELVNPYLETARLLGKRTAELHIALAGETDDPAFAPEPF
                     QVFYQRSLYQSMRNLTNKVFQTLAKRLKHLPAGIQPEAKAVLEKHPELLKRYQLFLNT
                     KISSVKIRHHGDLHLGQILYTGKDFVFIDFEGEPMRSAQERRSKLSPLRDVAGILRSF
                     HYAAYTVLAKKTSEKIVRPKDVPAVESWAYFWYMWVSSAYLRAYLDTAKDAPFIPRSK
                     EETAALLDIFILEKAVYELNYELNNRPEWTAIPLKGITRILEKKV"
BASE COUNT         2436 a         2757 c         2297 g         2320 t
ORIGIN      
        1 tcccggatct catgctgatc ggcactgctg aacccttggc cctggacatg aaaaatctgg
       61 aaaaggctta cgcgatcccc gaagttcgta acgacctcat agcccacgga attaaggatc
      121 ccgcgggtct cctttcagcc gcctggttat ttgatccgtc tttgcgtcgt ctcgcgcgag
      181 gcgccaaaat caactctgat aatcatccct atctggaatt ttccgctccc cgcagtcttt
      241 acaaagacac cgtggaggat aatttcaggc tcctgagtcc tttttaccgt ctcgatagct
      301 ttcctttgat ccgtaacctg gagccccccc aggataaaaa cccggcgttc tacaattccc
      361 tagcccgggg ctacctggtc aaaagttttt acgcgaacgc tgaaaacgct ttatccgtat
      421 cggaaaaaat tgatcccgcc catccggaaa cacggcttct tagaggaatt ttctattacc
      481 ttcttggcat gaaagaaagg gcgcgggaaa tactgatcca ggtcgtgata acttcccccg
      541 attgggcgga ggcttatgag tacctgggtt tgatctccca agattcggga aaattcacgg
      601 aggccgttaa ctcgttcagg aaagcatgcg aactcgagcc agaaaatatc gaccatcaga
      661 ttcgcctcgc gaacgcgctc cttaaagcgg gagaaaccaa ggaggcaatc gaagcttttg
      721 acaacattct gacaaggcga ccggaggatt ttgaaaatcg ggttaaacgg gccgagttaa
      781 tcgtcaaaac cggttctctc gaacaagggc tttcagccgc ccatgatgtc atcaagaaat
      841 acccccgctt catgggggcc tacgaatggg tgggaagtat tctggaaaaa aatgggcagt
      901 tggaccaggc cctggcagtt tacgacaaca tgctctccct ttttccaaat gatccgacac
      961 cccacattaa cctcgccaga ctaagtgaga gcttgggacg cccccaagac atgaaatggc
     1021 atcttgaaaa ggccgccgct cttaatcccg cgctcacgaa agtcccgtcc gtccgcaaac
     1081 tgctcgcctc agatcctcgc taaaccatcc ggtataccag aaacaatttc acaagcatct
     1141 gaaaagttgg aggttttccc gatctaatta tccctcgact tcactcccaa cgggcctgaa
     1201 gaccctccgg gacacagacg gaataacttt ctcggaaaaa gcgcccggga ggtcatttta
     1261 ttcattatca tgaaaagata atttgatgta aaataccccg cgaaagtgac cccgtagctc
     1321 agttggatag agcacctgcc ttcgtcacag gagcctttac gggaaaatga acctgtaaag
     1381 tggatgtcgc taattcggtg aatccccttg ttatcaaggg caacaccgag ctacccggaa
     1441 aatccgggga gtgtagagac tttacacgac agggctgaga cgcctaagag aaagtccagc
     1501 cagcaaaccc ggaaacgggc ggtcaaggta acttgatgct ggatgctaag cagggggtcg
     1561 cgtgttcgaa tcacgccggg gtcgattttt tacgctgttt tcctatccgt ttcttttcct
     1621 cgaaaaagcg ctggttgacc ggccataaaa catttccgcg cattccacca ccgtctcgga
     1681 caaagtcgga tgcggatgca ctctgcgtcc caattctttt gccgtcatct cgccttcaat
     1741 cgcaagcacg gtttccgcga tcaattcacc cgcgccttta ccgcaaatgc cggcccctat
     1801 gattttttca gtcgcgggat cgatcaaaag tttggtcacg ccatccgttc gatccatcgt
     1861 gacagccctc ccggacgccg tccagggaaa tcgtatcacc tggaccggag ttccggcgga
     1921 acgagcctca ttttccgtga ccccgcacca agcaacctca ggatccgtaa aaaccacggc
     1981 cggaatgagc gggaaagatt tcggggttgg gaatcccata atcgagtcaa tcgccagccg
     2041 agcatcacac gaggccttat gagcaagcat gacaccaccc gtgacatccc ctaccgcgta
     2101 aatcgagggg acggaagtcc gtttggctga atccgtccgg acaaatcccc tctcatccag
     2161 ccgaacccca atctcctcaa gcctcacctc tgccgtcaga ggagctcgcc cagtggaaac
     2221 taacacgcga tcaaacaact cttccgtgat attgcctccc gcgtcaatca cagctttgac
     2281 ttgattttgg caattttcaa ggcccgcgat tttagtgttc agcatgagct tttcaagacg
     2341 ccgctttgcc atctcttcga cgtaacggat cagatccgaa tctgcgccat tcaatatgcg
     2401 aggaaggact tccacgaccg tcactttact gccgagagcc gcgtaaacgg tccctagttc
     2461 catcccgata tatcccgccc cgatcacgag caacctttta ggaatctcct gaagctgaag
     2521 cgcttccgtc gaggtcatca cgagagggct agaaattttt agcgatttag gcagggccgg
     2581 cgtcgagccg gtcgcgatga tcgcatgctc gaaagtgatg cgcttatccg ccaccttaac
     2641 cgtccgctgg tccagaaacg tcgcgcgccc ctggatgatc tcaattcccc gccgaccgcc
     2701 aagatctttc attccccgac cgagtttttc gatcatctca gatttccaag cggtcagctt
     2761 cgcaagatcc aatgatggtt cggaaaatcc cagacccaat ttttcagccg ctcgcgcgcc
     2821 gtacaccgct tccgccacat ggagaagggc cttggaagga atacagccgc aattcagaca
     2881 ggtaccgccg agacgctcat ttttttctat caggataact ttttttccgc atgagaccgc
     2941 gtaagaggcg gacgcgtacc cgcccggccc tccgccaatc accactacat ctatttttaa
     3001 attttccatg actcccccat cgtaaggcgc gtcattctag caaaagaacg aaacgcgcgt
     3061 gtaaaagttt tcgcggctcg caacattcgc aagtcaaaaa aaaccgcgag cggccatctg
     3121 gagaagaaac aaaatttcct gaaactcgtt taaccccggt agcttggcct gttctgattt
     3181 cgcctgtaga gcgccgcgct ccagcttcgt ccgttcaata cgcggatcag tcgaagatga
     3241 tccgtcggaa actgttttcg aaaatcagga aaatgtcgtt tggaaattcc cgaaacgaca
     3301 agaaacccgc cggggcatgt cagggaagcc agcttttttc gatacctcag cagtgtcctc
     3361 gaaagcatat tggccccgac gaggtcgaac cttcccgttc ccgaaacttt tccgagattg
     3421 gccctgaaaa aacgcccgcc ctggcacccg tttctcacga aattttctct ggcaatcctt
     3481 gccgacatcg cgtcataatc aaaaccatgg atctcctccg cgccaagtct ggaggcgatc
     3541 accgacaata ctccgcttcc cgttccaaca tccaggaatc ttgtgaattt cccttccaga
     3601 ctttccatca tccgaatcat aagccgggtg gtttcatgcc cgcccgttcc aaacgcgctt
     3661 cggggatcca acacgaccgg aatccgtttt cctcctgttt ttccgatttt tttccaggcg
     3721 ggaacaatca aaaacttctt tcccacggaa atcttccggt aactttcctg ccatttgtaa
     3781 aaccagtctt tgggcctgag ccatttcccc tggacgcaaa gtccgcgaag gcgagaacac
     3841 ttcgcggctt ttgtgatccg ctgtctttgt ttcgtcgtta acgcgtaggc gctaacgcga
     3901 aaatatttct tataattcca gagaacaagt cccgccgaag ggatgcccgc gtcctcaaaa
     3961 aaccgttgga tctgatcctt aacctggcgt gacgcatttc gcgcgataat gctaaattca
     4021 ctgagattcg cgggaggacg agaaacagat tcggtcatca tctttatctg agcgaacaat
     4081 cgccaagttt aacaaatcga aaccctttgg attttagccg cggaacggtt tttcggagag
     4141 cgatcgcggc ctgaattccc gagtcctcca gcgtcatcgc gacgatagcg cccgaccgcg
     4201 cggggaaaat ttcttcgggc tttggagggt tctctgaatg gggatccggc atcattgaac
     4261 cgatcacttc ataccccatc gcccgggcca tccggacagc cacatcatca taatacgaag
     4321 aaggcgaacg aataaaacgg ggcaaggccc ctgtcaagga ttcaatcttt cgggcgtttt
     4381 tctcaatctc gatgaaaagc tcctcaacgc ttcgcgcggc cgacagttcc cgcgggacat
     4441 tttctccggt caccgtgcaa gctttccctt cgaggccgag gcttcctatt tcaaacaact
     4501 catcggaacc tattcgcttg atccattccg gatttttttc aatccacttt ccgctcacaa
     4561 aaaaaaccgc ggggatattc tccttgcgaa gaaaatcgag caacctcgta tcaaggggta
     4621 ccgattcgtt cgcgtttatg ttaaaggtca aagccattat attttcctga gaatcaatct
     4681 gggtcataac gcccgggacc accggccccg cctcatgcgg cttctcgtca gcaaattcaa
     4741 tcaggataat ttccttaagc aatcggtagt cgtctggttc cttaacatcg gaggcaaaag
     4801 ctgaccccgc gaccaacgcc gagattccgg cgccgatcac gatcaccctg aggcgcgaag
     4861 cgattctcgc agataacatg gttttatcgt ttccgttttt ctggataaaa ttcatcctga
     4921 aatttttctt aaaagacgcc gcacaagttc tcgcgggaga cccaccacgt tcgaatacga
     4981 tccctggatt ctttcgacaa ttctgggacc ttcctgaatt gcgtaggctc cggctttatc
     5041 gagaggccgg atggccagca aataatcacc gatttcttcg cttgtgagtt ttttgaattg
     5101 aacgcgggtg cacgaaacgc ctttcatcag gcgtttccgg acacgatccc acaagacaac
     5161 ccccgtgtaa acttcatgaa ccttccctga aagggaacgg agcattttgc gagcctcagg
     5221 caaacttttg ggttttccgt aaattttttt acgccagacc agagtgtcgg ccgccagaac
     5281 gtaccgggcg atccgtggaa ctttcgcgtg aatagccttc ccttccgcgt gttttaaaac
     5341 aagatcccga ggagcgcgtc ccggaataaa ccgctcttga taagaacttg ggacaacgcg
     5401 aaatcttatc ttcatcctcc ttaaaatttc ttttcttcgc gcggactggg acgccagata
     5461 aattatcacg atttattccc cgacccaggc gcaacgggaa ggcaagcaaa gggtttttcc
     5521 atccttcccg atttcagaca cgcgcccgca ggttccccaa cgacgggtat tcagtatttc
     5581 cctggaaact ccagcgtgaa aaggcagaac aatttcctgg ggacgatcca gaaaggaaac
     5641 cagtactttc attccctcgt cgtattcaaa tgcgagccac tgtttttctt catcaaaata
     5701 aaccccgctc agccggctcc ggccaagacg ctccttccgg agagtcatga gggtctgata
     5761 aagatgccag atttcggcgt gcgttccctc cttgtctttc cacgtcagtc gagaatcgaa
     5821 aaaagttttt tcactttctg gagcgggtat ttccttccag ccgaatgagg caaactccct
     5881 agtccgccct tccctgaccg cgcgaacgag atccggatcc ccatgatcga taaaatactg
     5941 aaacggggcc ttttcaccgt attcctggcc cataaaaagg agcggcgtat tcggtgaaag
     6001 gattaacaga actgccgcta ttttctcccg ctcgacatcc gtcaacgtgg atagccgatc
     6061 accccaggcg cggttcccga cctggtcatg attttgaaca cagacaacaa gtttccgagg
     6121 gtcgagatcc ttaacggggt tgccgtgaat tttttgacga aacgcggaat atcgtccatc
     6181 gtaaacaaat ccatcgctta acgctttggc aatatccgcc acaccggaaa aatccgaata
     6241 gtaaccgttc cgctctcccg tcaggcaccc atggacggca tgatgaaaat cgtcgctcca
     6301 ctgccccgcc aggccatatc ctttctcgtg gtacgggcgg atcacccggg aatcattgag
     6361 atcactctcc gcgataacgt gtacctcccg gcccaacacc cgggctagag attcgactcg
     6421 ctcattcagg tcacgaagaa tatgagttgg acttgagtcg aagataccgt gaatggcgtc
     6481 cagtcgcagg gaatcgacgt gatattcaga aacccagtag agggcatttt ctatcacaaa
     6541 ttggcgtacc ggttcgcagt cctgatcgtc ataattaagg gcgtctcccc agggcgtacg
     6601 gtatttcttt gtgaaataag gcccgtatcc gtgaaggtag tttccctcgg gccccaggtg
     6661 gttgtacacc acatcgagac aaacggccag ttcttcacga tgacacgcgt ccacaaattt
     6721 cttgaatccc tccggtcccc cgtaactatt ctgcgccgcg taaaggccta caccatcata
     6781 accccagttc cgggaacccg gaaactgagc gatcggcatg atttcaacac aggtgatccc
     6841 aagccttttt aaatagggaa gtttccggat agcggactca aaagttcctt caggcgtgaa
     6901 agttccgata tgcaactcat aaaaaatgag atccctgaac gcaatcccct tccatgcctg
     6961 atccgtccag gaaaaagccg ccgagtcaac tacgcaggaa ggcccgtgaa ctccttcggg
     7021 ctggtaccgg gaggctggat ccggacgatc ctcggttccg ttaagacgga aaaaatagcg
     7081 atcccccggc cgaactcctg acgcacgaac cctgaaataa cccaggggat ccttgcgcat
     7141 ttcaactaat cgggaaccgt cgtgattgat gagatgaagt tcgatttttt cacaacgtgg
     7201 ggcccacaca acaaatgaga cggttccatc cttttcaaga ttagcgccac acacaggata
     7261 gccgccgccc accgcgctac accttctttt ccaaaattcg cgtaatgccc ttcaagggaa
     7321 tagccgtcca ctcgggacga ttattcagct cataattgag ttcataaaca gccttttcca
     7381 gaataaagat gtcaagcaac gcggccgttt cctctttgct tcgcggaata aacggagcgt
     7441 ccttggcggt atccagatag gccctcaaat aagccgacga cacccacatg taccagaagt
     7501 acgcccagga ttcaactgcg gggacgtcct tgggacggac aattttttcc gaagtttttt
     7561 tcgcgagaac cgtataagcg gcatagtgga atgatcgtaa aatccccgcg acatcacgaa
     7621 ggggcgacaa cttcgacctc cgttcctggg cggaacgcat cggttccccc tcaaaatcaa
     7681 tgaaaacaaa atccttaccg gtgtaaagaa tttgccccag atgaagatct ccgtgatgcc
     7741 ggattttgac tgaggagatt tttgtattca aaaaaagctg gtatcgtttg agaagttcgg
     7801 ggtgtttttc aagaaccgct ttagcttcgg gctggatgcc ggcgggaaga tgtttgagtc
     7861 tcttcgcgag tgtctgaaaa accttattcg tcagattacg catggattga taaagcgagc
     7921 gctgataaaa aacctgaaaa ggctccggcg cgaacgcggg atcatcggtc tcacccgcca
     7981 aggctatgtg caattctgcc gttctcttcc caagcaaccg ggcagtttca agatacgggt
     8041 tgaccaattc agtcgcgaga gaatccactt ccccctgcgc gagctccatg aggctttttt
     8101 cacaactttg ccttttttta aatttatcgg gacgcgcaaa tactccgtca aaataactgg
     8161 cgagcgcgtc cagcgtatat tgccacgcat ctccctgatt tttgataaac tcctgaagca
     8221 cgccaatcgt cgcgtaagaa cttcgaccac ggctatactc caaagagcca agcatcctgg
     8281 ggacattaac aaaatccgtg ttttcggtga gataacggcc catttcgaaa tctggattga
     8341 ttccttcttc tatcttccga aaaagtttca gaaccatccg tcctccataa ttgatagacg
     8401 tgttactctg ttccgccttc atcagggagg attcgcgcaa agacgaatct ttaggccaca
     8461 acttcttgag ctccgccgtg ggtgtcccct ggattactcc ctgcgctccc ttgaaccggc
     8521 ggcggcgatg aatgccgtca agaatcgccg aggtaaaagt cctgtcgacg atggcctcat
     8581 aaaggacacc atccgtgatc ccgccactgc ccttgacttc aacttgagcg atccgggaca
     8641 gcggaaactg ggtctgtatt tcacccgctc gggaacctga cgcgtatgcc acgggaagca
     8701 aataatactc cgcctcacct tccgcgtact ctaccttgag aaacacgaga aatgaatcac
     8761 cggcgttaca tggtactgtc actgcttccg ttatctggac acgttttagt tttcgggctt
     8821 tgccggcaaa ccagcgacag gcccgaatat agtcgggaag tagcgactcc aaatgcgttt
     8881 ttgcgtctcc tttgaacata cgggtccagt gatctttcac aacaatcttt ttgagttccg
     8941 tgggcgggga cgaaatgagc gccggttgag ctttgggggc cgtttcgaga ctaaaccagt
     9001 aaaaagagtg aggtccgagc gtcacaaagt aagggagctc tccgatggaa ggaaattcat
     9061 tatgcccgaa catttcaacc gggaccattc ctttataagc cgaaagatcg atttcaacac
     9121 attgaacaaa tcttgaaagg ttggcgatga cgagaatttg ttcttcccgg tacttgcgga
     9181 taaacaccag cactttcccg ttttcaggag acaaaaactc gatcgaccca cggctgaaag
     9241 ccttgtaccg cttccggagg gcaatcaggc gtttcatcca ccagagtagc gagtgcgtat
     9301 taccctgctg ggcttccacg ttgacggtcc cgtagtcgta ttccggatca ataatgacgg
     9361 gcagataaag tttttgggga ttggcacgtg aaaatccggc gtttcggtcg gcgctccact
     9421 gcatgggtgt cctgacaccg tttcgatccc cgagatggat gttatcgccc atccccagct
     9481 catctccgta ataaatcacg ggagtcccgg ggagcgaaaa aaggagccca ttcatcagtt
     9541 caattttccg tcggtggttc cccaataaag gggcaagcct tcgacggatc ccgagattaa
     9601 ttcgggcctt gggatcgtgg gcgtagacac ggtacatgta gtcacgctct tcatccgtca
     9661 ccatttcgag tgtcaactcg tcgtggttcc gcagaaaaat cgcccactgg caggaatccg
     9721 ggatctccgg cgtttgctga agaatgtcga cgatcggaaa acggtcttcc atgtggattg
     9781 ccataaaaag ccgcggcatg atgggaaaat
//
LOCUS       DAOSIO010000043         9804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_6689_length_9804_cov_3.523438, whole genome shotgun sequence.
ACCESSION   DAOSIO010000043 DAOSIO010000000
VERSION     DAOSIO010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 9804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9804
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6689_length_9804_cov_3.523438"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3698
                     /locus_tag="PK590_02805"
     CDS             <1..>3698
                     /locus_tag="PK590_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23559.1"
                     /translation="GSVQKPEETSSETTPQVDGKRGALGVPDSNGMDKLYQQKGRRSK
                     EGVEISLARALALDENLPDLAAMREELKLRLEVQKAAGKYSFNDVVNDDSLASWAKEF
                     RKIQERFYPKEDFPDFTVDKLFEIAESAMGLRAKAGRAIAVDFASFSNGLSQPSWMMP
                     EDLNTIEARVKDLAKGRVTLEDAVELFRDFAREQKLKGQSKEGYGSAVSWMQERAKQR
                     LLLQAAYQVSHKDSQGRRMTAEDSDISPWVTLVKNGFAPLYEFAGILKYDGTFQQKEL
                     ALRDAVVKAYKEKNGSATPAELEAMKASFPIMDDWQRMGFAARMYELGHQTDWLDRWF
                     ASVEKIQLASWTHTGHFIDPMLISFAVKRFARADGSMDWLDNWRMNKYLATIEAYNDL
                     AFMDLGSEAGDLARLLWSWRVVFNAVPTEKERENLLSLWKDLGKQDYLKTASVSERQT
                     AERSLVSRILMVRDTFSDVYAANHGNEEPPQELLEAITGIAAQPILRMLDRMAERRDG
                     VIAFRAEQDKEKAALEPHRVSSRDWYSRKQETVDVKTLLTNLTTIAQGYSMSLSEADR
                     TLLMKRVDKDTAQMLSEKMQLRGYDAHDAFVLYVWLPFRVEKVYREVSRIGGESFLTS
                     DQRGEVAAYASMIFRDWGFLLNEGHEKERLEAEEALSKTIEQGITGRIEQLRIQIERS
                     RIYKEKFDTAFPQLGTQLTSQDAFLWAEKARSSALSDDEFQQWIDLAKELKVVDDSLT
                     IKQALALADQWLRIGLAEELPEHYWNQDEAETIFKATFTNEQFKQAQKSGPEDSEVLR
                     GWLQRRDRGFRSLDDPELITALAQGSVSRNISPLLFARRAQQAIYARDMIDRAAQFNN
                     ALSKNDKRYDPAGIERFSVSDLLVYYDAIAPNSKGFDLVSTLEQTGRGEKVFGEQANR
                     LWEDRVEASINAAFRVEHEKILRPLLPPATEPAVRQEEKKEAPSLDQRIRKLIWEKIQ
                     EPALKALPGTNDLTTSEAPSRSEMRVSMELGFSSFVQFLWHSVALTNALWITLIVGVI
                     VFLYDFVHKSAFSRAIGDEASRPGFWKWRAFWSVTFFTTVLTQMVYISVINPGAYYLT
                     ALTASVLISVYLIWAISYKFWGWIITSLYPLYSLIVDGLWKRGILRDKSSRVTAEPIM
                     RPIFNADDSDFQLVSENGRRIYYLTEAFFEREYPEASVREDMKAKLGWVGRDGGVHPG
                     TQINAVQMLLRPDRDDKYPEEQESIAFW"
     assembly_gap    3699..3708
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3779..5029
                     /locus_tag="PK590_02810"
     CDS             3779..5029
                     /locus_tag="PK590_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23560.1"
                     /translation="MIHLFARDVDYDEFLNLDIDRGLLIATLSGASRRASDDVFWEVL
                     QSQLEQISDLNGVTDSQFIESINRILLMPGFYDRVSSQVNVNTLDPEVQTLAEKAIRA
                     QAEGGTKLSSKERRALNKALLLQIYSTEIQPTNRNGWLKEGLSDAKQDDDKTTYLLRN
                     LKNIRMNDSIRGEYYLDLLKEYGERVLVFVRHGPLEKPFDYQAEFRWLVTGTANLFGR
                     INANDHAIADVNIDRDEFWPLWEGFNGKMITVVDSKGGAHKIRIPIPVIDGSLTPDGR
                     LLFLDARHKEECYRMQLYIERAKWLINKAIEENKEPPQFEGYGDTHNALMTWVLEQPI
                     DSARMDAWDSRGYDERLSGPQSRFRNKVPGKSEVYSVTPFDAEIGMSAKDFVKGIAKA
                     NQPLGHAPTFTDHEEFSCDASDLF"
     gene            4971..>9804
                     /locus_tag="PK590_02815"
     CDS             4971..>9804
                     /locus_tag="PK590_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23561.1"
                     /translation="MLQPSQITRNFLATLQTFSEATAQGSLWHLHTNLMIIFKAIRGF
                     GKFFIVVDRYYKNSMRPTLAERPAQAGNKLLGWANTFLKGKQKDALYGRYMTPAKEAS
                     EPIRNPRLFLVFIWTCALIPLGVFVFVGLTAGLAAGIIPALLTAVGASVAMIYGVKFW
                     PKDHSGGNPRAVQSHDEFESLQNFIGLMLDVHMYDDAKPSFLGWVARLFRWLGWFDAM
                     TIYTRFSGAQKYPVSNRVFNNGCVRGLLATPLWGVLLLIGLVSVKVPGIQEITVPGIA
                     EWLFVSIMFMLLMDKWVAPTYNYFKRLGWWSVPAVGLFVAYFAIPILIWGPVAALGTV
                     IVFMTSAAIMYYAQYVSGMKSKAGTWLSVGIGVLATVGLFFVGWNLVAAVLVGTVIFA
                     VLHSSVYLVRKILRSDTGYHARYGENYNLDNIFLRVGEEVTSKEILEDAEVGMLPEDY
                     RDYKNAVQPEAWKKILFMPLILGSYWNHAFGEFISSTLTLLQYLWWTPIYLLKAIQNV
                     VGRLLIKKEVTSLNQPKFDSKNVFDFVRAGRGVVTIGVLLMFFSMGLGWMGAAGLGFL
                     EWAGWRVILTVGISGIFLMRYPRILAKYKKALEEIKKLDKTIEDLEKSRISDKSMSIE
                     HKIAAAVEARNNQMLAVKVAKKDLYLYGWLSIAILIVGAVFAPSLAGFVDRHWIVRAL
                     PIHLAFLFGSVVAFMMSVENFFYKRKLESKNVSVAKVDQVSPRGVELAETEVQAIIEK
                     SSVKVVYGLLGYATFIGTVLYFTYLEPIPEFHLLIRNFMEKMGAFFVAAMPADGFNLV
                     TPGNMMEGIGRILHTNPFFVALQISVMIGGGFWMHAAVIVGLIHWFRNPKASELRKLL
                     TGKGESWKNFKEILKLSRELKERDLLRQQKGGKRSEVRTAASTDAEQEALIQSVRAMT
                     DSELDATATRILADRFRELMQKGVMAALSPDSKLLFYSAGPATGKGTVLKTTFLAEDG
                     DVQAPYQDLVKKFVIGHTRKFRPHDKEKDGRHYHFRTAEHWEALHKRLGADYLELRYI
                     NKQLQVLPIKTFVNPGVVVPDVQNATLLEPKDVVLSYDIPEGQLFRTELKVARPGVGE
                     ITLNDIIIPADKPGTFLPRDEVVSVSGDTVIVNRVIVGLRDLLNGNHFTIFEGGYDWF
                     RWVRDPANHSGALTLFLSPFEEADFPKRASNMNWILTAKDANGEALFPLPKDVYLAFE
                     AIDALVEGKGDQQPLGEFDIADRADLQERVKVMVRESQEQVTPLLEANEAIILDDKDF
                     IRELNGVMTGFGASALNTKDYMLARALANELQHRLFGRDGAKSFRDESGNRTDSYNRI
                     LEGVFQIMTRANYGSANVLPNARIYDVHERKVALAKLGDKFARRLFEHAIDGIRRSEL
                     RDSEIEEFSTPVRSEVRENLDRLVRGELTPFEIEQLPLDVKTEILRGLVSGVAGQTLA
                     DHRLQEKMLVLGGPPGSGKDLGAKSFIGEGIPLEGDGMLSFKDLFGGIILYHSRAPRF
                     VKGKWEINDIKYGFADRRVGDPRNRILRLSQLRYDDGTPYAIKAFVNKQLQGVATRRF
                     EEIETLFSTRGSDELFEGEVPLKTRTFRGVTAHRVVRSIKGLEDAFSANNLTYLEGGY
                     H"
BASE COUNT         2406 a         2049 c         2817 g         2522 t
ORIGIN      
        1 cgggcagtgt ccaaaaacct gaagaaacat ccagtgaaac aacgccccag gttgacggaa
       61 aacggggagc gcttggtgtc ccggactcga atggcatgga taagctctat cagcagaaag
      121 gacgccgtag caaagagggg gtcgaaatat ctttggctag ggcactggcg cttgacgaaa
      181 atttaccaga tttggccgcc atgcgcgaag aattgaaact tcgtcttgaa gttcagaaag
      241 ctgcagggaa atatagtttt aacgatgtcg taaatgatga cagtttggct tcttgggcca
      301 aagagtttcg gaaaattcag gagagatttt atcccaaaga agatttccct gattttacgg
      361 tggataaatt atttgaaata gccgagagcg cgatgggttt gagggctaag gctggaaggg
      421 ctattgcggt ggattttgcc tcgtttagca acggactgtc ccaaccttcg tggatgatgc
      481 ccgaagattt gaatacgata gaagcaagag taaaggatct tgccaaagga cgcgtgacct
      541 tagaggatgc agtggagctt ttcagggatt ttgctagaga acagaaactc aaaggccaat
      601 cgaaagaagg ctatggcagt gcagtttcct ggatgcagga gcgtgcaaag cagagactgc
      661 tcttacaagc tgcttaccaa gtttcgcata aggattctca gggtcgcaga atgaccgcgg
      721 aagattcgga catatcgccg tgggttactc tggtgaagaa cggatttgcg cccttatatg
      781 aatttgcagg gattttaaaa tatgatggga ctttccagca aaaagaactt gcgctcaggg
      841 acgcggtagt taaagcatat aaagaaaaaa atggaagtgc gaccccggct gagttagagg
      901 caatgaaggc gagttttccc attatggatg attggcaacg catgggtttt gcggcccgca
      961 tgtacgaatt gggtcatcag acggattggc ttgatcggtg gtttgcgagt gtggaaaaga
     1021 ttcaattggc atcgtggaca catactgggc actttataga tccgatgctg atcagcttcg
     1081 cggtaaagcg ttttgccagg gcggacgggt caatggattg gctcgataat tggagaatga
     1141 ataaatacct ggcgacgatt gaggcgtaca acgatcttgc attcatggat ttgggctccg
     1201 aggcgggaga ccttgcccgg ttgctctggt cgtggcgtgt tgtttttaat gcggttccga
     1261 cggaaaagga gcgagaaaat ctgttgtctc tttggaagga cttagggaag caggattacc
     1321 tgaagacggc ttcggtgagc gaaaggcaaa cggccgaacg ctctctggtg tctcgtattt
     1381 taatggttcg agataccttt tcggatgttt atgcggccaa tcacgggaat gaagaaccgc
     1441 ctcaggaact cctagaggcc attactggga ttgccgctca accgatcctc aggatgcttg
     1501 accggatggc cgaaaggcgt gacggtgtca ttgcgtttcg cgcggaacaa gataaagaaa
     1561 aagcggcttt ggaaccgcat cgcgtttcat ccagggattg gtactctcga aaacaagaga
     1621 cagttgatgt caaaacattg cttacaaatt taacgacgat agcccagggt tacagcatgt
     1681 ccctttcaga agctgatcga acccttctca tgaagcgtgt cgataaagat acagcgcaga
     1741 tgctttcgga aaaaatgcaa cttcgcggtt atgacgccca cgatgcgttt gtcctttatg
     1801 tctggctacc gttccgggtg gagaaggtct atcgtgaggt ttccaggatt ggtggggaaa
     1861 gcttcctgac cagcgaccag agaggtgaag tggctgcata tgcgagtatg atcttccgtg
     1921 attggggctt tctgctgaat gaaggccatg aaaaggaacg gcttgaagcg gaagaagcgc
     1981 tcagcaaaac gattgaacag gggatcactg gtcggattga acagttgcgg atacagatcg
     2041 agcgttcccg gatctacaag gagaaattcg atactgcatt tcctcagctt ggtacccaat
     2101 tgacatcgca agacgcgttt ctgtgggcag aaaaagcgcg tagtagcgcg ttgtcggacg
     2161 atgagttcca acaatggatt gacctggcga aagagctgaa ggtggttgat gattccttga
     2221 caataaaaca ggctctggcg cttgccgatc agtggctcag aattggtttg gcagaagaac
     2281 ttccggagca ttattggaat caggatgaag ccgaaacgat ttttaaggcg acatttacga
     2341 atgagcaatt taaacaagcc caaaaaagtg gaccggaaga ttcggaggtt ttgcgcggat
     2401 ggttgcaacg tcgggatcgc ggttttcgct ctttggatga tccggagttg attacggctt
     2461 tggcccaagg atcggtttcc cgcaatattt cgccattact cttcgcacgg cgggcgcaac
     2521 aggcgatcta tgcgagagac atgattgatc gagcggccca attcaacaat gcgttgtcca
     2581 agaacgacaa gcgttatgac cccgcgggga tcgaacgttt ctcagtttcg gatctccttg
     2641 tttattacga tgcgatagcg cctaattcga aaggttttga tcttgtatcg acactggagc
     2701 aaactggccg tggagaaaag gtttttgggg aacaggcgaa ccggttatgg gaagaccggg
     2761 tggaggcctc gattaatgcc gcgtttcgtg tggaacacga aaagattctg aggccattgt
     2821 tgccgccggc aacggagcct gcggtacggc aggaagaaaa aaaggaggct ccatcgctag
     2881 atcaaaggat tcgaaaactg atctgggaaa aaatccaaga acccgcattg aaggctttgc
     2941 cggggacaaa tgatttaacg acatcggaag ctccttctcg ctctgagatg cgtgtgtcca
     3001 tggaacttgg ttttagcagt ttcgtccagt ttttgtggca ttcggtagcg ctgacgaacg
     3061 cgttatggat cactttgatc gtgggtgtca ttgttttttt gtatgatttt gtccacaaat
     3121 ccgcgttcag ccgtgcgata ggtgacgagg caagccgccc tggtttttgg aaatggcgtg
     3181 cgttttggag tgtgactttt tttacgacgg tcctgaccca aatggtttac attagcgtga
     3241 tcaatccggg agcctactat ctgacggccc ttacggcttc ggtcttgatc agcgtatatt
     3301 taatctgggc catttcctac aaattttggg gttggatcat aacctctctt tatccccttt
     3361 attccctgat cgtggacggc ctttggaaga gaggaatcct gcgggataag agttcgcgcg
     3421 tgacggcgga acctatcatg cggccgattt tcaatgcgga tgactcggat ttccagcttg
     3481 tttccgaaaa cggacggcgt atttattatc ttaccgaagc tttctttgaa cgtgaatacc
     3541 ctgaggcgtc tgttcgcgag gatatgaaag ctaaactagg atgggtcgga agagatgggg
     3601 gtgtgcatcc ggggacccag atcaatgcgg tccagatgct tttacgtccc gatcgcgatg
     3661 ataagtatcc ggaagaacag gaatctatag ctttctggnn nnnnnnnngg cacgctgccc
     3721 aggaccgcgt ccaaattttt ccgccacccg catgatgggt cggttgccgg acaattacat
     3781 gattcatctt tttgcccgcg acgtagatta tgatgaattc cttaatttgg acatcgatcg
     3841 gggtttgctg atcgcgacct tgtcaggagc gtcccgtagg gccagtgatg atgtgttctg
     3901 ggaagtatta cagagccaat tggaacagat ttccgatctc aatggtgtga cggattcgca
     3961 gtttatcgaa tcgatcaacc gtatcctgct catgccgggt ttttatgatc gtgtcagctc
     4021 ccaagttaat gtcaacaccc tggatcccga ggttcagact ctggccgaaa aagctattcg
     4081 ggcccaagcc gagggcggta cgaagctcag ttcgaaagaa cggcgggcac tgaataaagc
     4141 actgttgctt cagatctata gtactgagat ccagccgact aacaggaatg gatggctcaa
     4201 ggagggattg tcagacgcaa aacaggatga cgacaagacg acgtacttgc tcaggaatct
     4261 caagaatatt cgaatgaacg attcaatccg tggagaatat tacttggatc ttctcaaaga
     4321 gtatggggaa agggttcttg tttttgtccg tcacgggcca ctcgaaaaac cttttgatta
     4381 tcaggcggag ttccgctggc tggttacggg tactgcgaac ctctttggtc ggatcaatgc
     4441 caacgaccac gcgatagcgg acgtgaatat tgatcgggat gagttctggc ctctgtggga
     4501 aggatttaac ggcaagatga ttacggtggt tgattcgaag ggcggtgcac acaaaatcag
     4561 gattccgatt cctgtgatcg atggaagcct cacgccggac ggccggcttt tgttcctgga
     4621 cgcgcggcat aaggaggagt gctaccgcat gcaactgtat attgaaaggg ccaagtggtt
     4681 gatcaataag gcgattgagg agaacaaaga gccccctcaa tttgagggtt atggtgacac
     4741 tcataatgca ttaatgacgt gggtattgga acaacccatc gattcagcca ggatggatgc
     4801 gtgggactcg cgaggatatg acgaacggct gagcggccct caaagcagat ttagaaacaa
     4861 ggttcccgga aagagcgaag tttattccgt gacaccgttt gacgcggaga tcggaatgtc
     4921 ggcaaaggat tttgtgaagg ggatcgcaaa ggcgaaccag ccgctgggcc atgctccaac
     4981 cttcacagat cacgaggaat tttcttgcga cgcttcagac cttttctgag gcgacggcac
     5041 agggatctct ttggcatctg cacacgaatt tgatgatcat tttcaaggcg atccggggat
     5101 tcgggaaatt ctttatcgtt gttgaccgct attataaaaa ttcgatgaga cctactttgg
     5161 cggaaagacc ggctcaagcg gggaacaaat tgcttgggtg ggcaaatacc tttctgaagg
     5221 gaaaacagaa ggacgctctt tacggtaggt atatgacgcc ggccaaggaa gcgagcgagc
     5281 cgatcaggaa tcctagactt ttccttgttt ttatctggac atgcgccttg attcctctag
     5341 gcgtattcgt ttttgtaggg cttacggcag ggcttgcggc agggatcatt cctgcattgc
     5401 ttaccgctgt tggagcgtct gtggcgatga tttatggtgt gaaattctgg cctaaagatc
     5461 attcgggagg caatccgcgc gcagtacaaa gccacgacga gttcgaatcc cttcagaatt
     5521 tcatcggatt aatgctggat gttcatatgt acgatgatgc caagccgagt ttcctaggct
     5581 gggtggcgcg tcttttccga tggctcggtt ggtttgacgc gatgaccatc tatacgcgtt
     5641 tcagtggcgc ccagaaatat ccggtttcga accgggtctt caataacggt tgcgttcgcg
     5701 ggcttttggc gacgccgctt tggggcgttc tccttcttat cgggcttgtt tccgtgaagg
     5761 ttccgggtat ccaggagatc acagtaccgg ggattgcgga atggctattc gtgagcatca
     5821 tgttcatgct actgatggat aaatgggtgg cgccaaccta taactatttc aagcggctgg
     5881 ggtggtggag tgtgcctgcc gtcgggttgt tcgtggccta ttttgccatc ccgatactta
     5941 tttgggggcc tgttgccgcg ttgggaacgg tgattgtttt catgacctct gccgccatta
     6001 tgtattacgc gcagtatgtt tcggggatga aatccaaagc gggcacttgg ctttcggtcg
     6061 gaattggggt tttggcgacg gtcggcctgt tttttgtggg atggaatctg gtcgccgcag
     6121 tgcttgtcgg tacggtcatt ttcgcggtat tgcatagttc ggtttatctt gtgcgtaaaa
     6181 tactgcgttc agatacgggg taccatgccc ggtatggcga gaattataac ctcgacaata
     6241 tttttctgag agttggcgag gaagtgactt ccaaagagat tctggaggat gccgaagtcg
     6301 gtatgcttcc ggaggattat cgggattaca agaatgcggt tcagcccgag gcatggaaga
     6361 agattctgtt catgccgctc attctcggaa gttattggaa ccatgcgttc ggggaattta
     6421 tctcctccac gctgacgctt ctccagtacc tgtggtggac accgatttat ttattgaagg
     6481 ccatacaaaa cgtggtgggg cggcttctga tcaaaaaaga ggtgaccagc cttaatcagc
     6541 ccaaatttga cagtaaaaac gtttttgatt ttgttagggc gggtcgtggt gtggttacga
     6601 ttggtgttct tctgatgttt ttttccatgg ggcttggatg gatgggtgcg gctggtcttg
     6661 gctttcttga gtgggccggg tggcgtgtga tcctgaccgt tgggatcagc gggatctttt
     6721 taatgcgcta tccgaggatt ctagcaaaat acaaaaaagc cctcgaagaa attaagaaat
     6781 tagataaaac gatagaagat cttgaaaaga gccgcattag tgataagagt atgagtatcg
     6841 aacataagat cgcggcagcc gttgaggcaa ggaataatca aatgcttgcg gttaaggtgg
     6901 caaagaagga tctttatctt tacggttggc tttcgatagc cattttgatc gtgggagctg
     6961 tttttgcgcc cagcctggcc ggttttgtcg atcgccactg gattgtgcgc gcacttccga
     7021 tccatcttgc gtttttattt ggctcagtcg tggctttcat gatgagcgtc gagaatttct
     7081 tttacaaaag aaaactcgag tccaagaatg tttcggttgc taaagttgat caagtatccc
     7141 cgcgcggggt ggagctggcg gaaacagaag ttcaggcgat cattgaaaaa tcgagtgtga
     7201 aagtggtcta tggattacta ggttatgcaa cgtttattgg tacggtgctt tactttacct
     7261 atcttgaacc gatacccgaa tttcatcttt tgatccggaa cttcatggaa aaaatgggcg
     7321 ccttttttgt cgcggcaatg ccggcggacg gatttaatct cgtgactcca ggaaacatga
     7381 tggaaggtat cggccgcatc cttcacacca atcctttctt tgtagcgttg cagatttccg
     7441 tcatgatcgg tggcgggttt tggatgcatg ccgcggttat tgtgggattg atccactggt
     7501 ttagaaatcc taaagcatcg gagcttcgta aattgctcac cggtaaggga gaaagttgga
     7561 aaaacttcaa agaaatttta aagctaagtc gtgaacttaa agaacgcgat ctcctgcgcc
     7621 agcagaaggg cggcaagcga tcggaagtga ggacagcggc gtcgacggat gcggaacaag
     7681 aagcgctgat ccagtcggtc cgtgcgatga cggattcgga gctcgacgcg acagccaccc
     7741 ggattcttgc ggaccggttt cgcgaattga tgcagaaggg tgtcatggcg gccctttctc
     7801 ctgacagcaa gcttctcttt tattccgccg gtcctgcgac gggaaaaggt accgtgctga
     7861 agaccacctt ccttgctgag gatggcgatg ttcaagctcc ttatcaagat cttgttaaaa
     7921 aatttgtgat cggtcataca agaaagtttc gcccgcacga taaggaaaaa gacggtcgcc
     7981 attatcattt cagaacggcg gaacactggg aagcgctcca taaacgattg ggcgccgatt
     8041 atctggaact acgatatatt aacaagcaac ttcaggtgct gccaataaag acctttgtca
     8101 atcccggtgt tgtggttccg gatgttcaga acgcaacgtt gcttgagccg aaagatgtcg
     8161 tgctcagtta cgatattccc gagggacagc ttttccggac ggaattgaag gtcgctcggc
     8221 cgggagtcgg tgaaattacg ttaaacgata tcataatccc tgcggacaaa cccggaacgt
     8281 tcctgccgcg ggatgaggtt gttagcgtgt ccggtgatac agtgatagtg aatcgcgtga
     8341 ttgtcgggct gagagatctt ttaaacggca atcatttcac gatttttgag ggcgggtatg
     8401 attggttccg gtgggttagg gatcctgcca atcactccgg ggcgttgacg ctgtttcttt
     8461 ctccgttcga agaagcggat ttcccgaaac gggcaagcaa tatgaactgg atattgacgg
     8521 cgaaggacgc gaacggcgaa gcgctttttc cgttaccgaa ggatgtctat ctggcgtttg
     8581 aggcaatcga cgcgctggtg gaagggaaag gagaccagca accgctggga gaatttgata
     8641 tcgcggacag agcggacttg caagaaagag tgaaagtgat ggttcgggaa tcccaagagc
     8701 aagtgacacc gcttcttgaa gccaatgaag ccattattct tgatgataag gattttatcc
     8761 gggagttgaa cggggttatg accggttttg gggcatcggc attgaacaca aaagattata
     8821 tgctggcccg cgcactggcg aacgagttgc aacatcgtct gtttggccgt gatggagcca
     8881 agtcgttccg tgatgaatcc ggaaatcgga cggattctta taaccggatt ttggaaggag
     8941 tgttccagat catgactcgc gcaaattacg gttccgcgaa cgttctcccg aatgcgcgca
     9001 tatatgatgt tcatgagcgg aaggttgctc tggcaaagct tggcgacaaa ttcgctcgtc
     9061 ggctttttga acacgccatt gacggtattc gtcgttcgga gcttcgtgat tcggagatag
     9121 aggaattcag cacgccggtc cgatcggaag tgcgtgaaaa tctggatcgg cttgtgcggg
     9181 gggaattgac tccgtttgaa attgaacaat tgcctctcga cgttaaaacg gaaatacttc
     9241 gcggacttgt gagcggtgtt gcggggcaaa cgctggctga tcaccgtctg caggaaaaaa
     9301 tgcttgtgct cggcggacct ccagggtcag gaaaagatct tggggcgaag tcgtttattg
     9361 gagaagggat tccgttggag ggagatggaa tgctgagttt taaagatctt ttcggcggaa
     9421 tcattcttta tcatagccgc gccccgaggt ttgttaaggg caaatgggaa ataaatgaca
     9481 taaaatacgg tttcgcggac cggcgtgtcg gagatccgcg gaaccggatt ttaaggctta
     9541 gccagcttcg ctatgacgat ggtacgccgt atgcgatcaa agcgtttgtc aacaaacaat
     9601 tgcagggtgt cgcgacgcgt cgattcgagg aaattgaaac gttgttttct acccgtggct
     9661 cggatgagct ttttgaagga gaagtgccgt tgaaaacgag aacgttccgg ggagtgacgg
     9721 ctcatagggt tgtccggtcg attaaagggc tggaagatgc atttagtgct aacaatctga
     9781 cttatctgga gggtggttat cact
//
LOCUS       DAOSIO010000044         9772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_6714_length_9772_cov_4.693115, whole genome shotgun sequence.
ACCESSION   DAOSIO010000044 DAOSIO010000000
VERSION     DAOSIO010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 9772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9772
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6714_length_9772_cov_4.693115"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="PK590_02820"
     CDS             complement(<1..1007)
                     /locus_tag="PK590_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOG23562.1"
                     /translation="MNEKILLRPIEEEMKDSYIDYSMSVIVGRALPDVRDGLKPVHRR
                     ILFAMNELGLAHTKPYKKSARIVGEVLGKYHPHGDTAVYDSLVRMAQDFSLRYPLVQG
                     QGNFGSIDGDSAAAMRYTEARLASAAGELLADIDKETVDFVPNFDESLSEPALLPSRI
                     PNLLVNGSSGIAVGMATNIPPHNMTEVVNACVALIDNPDITNKELFKIVTGPDFPTGG
                     LIVGRDGIKKAYETGRGAIVMRAKAAIETGRNKKDAIVITEIPYQVNKSNLIESIVRL
                     VEDKRIDGISDLRDESDRDGMRVVIELKREAVPQVVLNNLYKHTQMQSSFGVIMLALV
                     DG"
     gene            complement(1127..3661)
                     /gene="gyrB"
                     /locus_tag="PK590_02825"
     CDS             complement(1127..3661)
                     /gene="gyrB"
                     /locus_tag="PK590_02825"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOG23563.1"
                     /translation="MTKSDQSKPGLSGAKYDATKIQVLEGVDAVRKRPAMYIGDTTLR
                     GLHHLVYEVVDNSIDEALAGFASRVDVVINADGSCAITDDGRGIPVDMHKTQKKSALE
                     VVMTTLHAGGKFDNKSYRVSGGLHGVGVSCTNALSSWCEVKVRRDGKIWQQRYEKGRA
                     VSKVIEKGKTKEHGTTVVFLPDKEIFEKTEFSFDVLSNRLRELAYLNKGVRVTIRDER
                     NKKEIKEHVFMYKGGISEFIQYINRSKTPLHRKIFYVDKTKDGVEVEAALQYNDGYGE
                     DVFSFANNINTIEGGTHLSGFKTSLTRATNQYAKSKNLLKNLEGGLSGTDLTEGLAAV
                     ISVKVPRPQFEGQTKTKLGNSEVEGIVYSVLYESLSTFFEENPPVANKIVEKAALALR
                     AREAARKARELTRRKGALESASLPGKLADCSDEDPANSEMYLVEGDSAGGSAKQGRDR
                     RFQAILPLRGKIINVEKARIDKVLSNKEIRTIITAIGTGIEDEFNIEKLRYHKIILMC
                     DADVDGSHIRTLLLTFFYRKMRKLIENGHVYIAQPPLYKIKRGKDEQYIQTEDQMNGI
                     LLNFGLQGTLLKKHGSKGSVSGKDLRELMDLVQAMEDLERSLARKGISLHKFVENQDK
                     KKGFPTHVVRLFEKNEDVFLYSEKEATAYTEEIEKEYVKKLEQDRKDDAKEGEKAKKK
                     DVKKGKKVKEEDAKEDEDDISFQSVYDLIEIFESVEFAKAKKKLQKFNINFEDFEADK
                     EPLFEVVFEKNGKEEAPNLRSVLSKIKTWGKEGMTLQRYKGLGEMNPEQLWNTTMNPE
                     TRTILRVTLEDAVEADEIFSILMGDAVEPRRAFIERHAKAVRNLDI"
     gene            complement(3666..3974)
                     /locus_tag="PK590_02830"
     CDS             complement(3666..3974)
                     /locus_tag="PK590_02830"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HOG23564.1"
                     /translation="MSIKRCEIECIRSVIPSVIRGLENPEKKTRSLLIEKWPAIAGGK
                     LTPHTRPQLTQKGILYVYVSEATIAYEISQKYRHSLLKRTQVVLGEDAVKELKVLVGK
                     "
     gene            complement(4059..5159)
                     /gene="dnaN"
                     /locus_tag="PK590_02835"
     CDS             complement(4059..5159)
                     /gene="dnaN"
                     /locus_tag="PK590_02835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOG23565.1"
                     /translation="MDIRIPKNELLKKIGLGVSIAGGKPSTLPILNNLLLETQKDGML
                     KIVATDMEVGVSTLLPVEIGQQGSVTVPARKFHDIVKELPEGEISLSVTKNNTVNIKS
                     GKSFFKIMGLDKEDFPKLPDPSPEQEVELEQSILKESLILTSFAISHDETRYVLNGVL
                     VSLQENQIRFIATDGRRLAFYKKDLKEKIGKKIDMIIPSKTIHELLKLLEWEGKVKIS
                     HSQNKVIFKFQDTYLTSSLIEGNFPNYEQVIPKEEKTTAVVHCEEFLQAVRRVALLTS
                     IESPAVKFDFIKGKVLISAKSPNMGEAREEVPAEVSGQEVTIGFNPNYFIDLLKNLTD
                     ESITFALTEPDKPGLVKGRNGYLYVIMPMQLS"
     gene            complement(5343..>6204)
                     /gene="dnaA"
                     /locus_tag="PK590_02840"
     CDS             complement(5343..>6204)
                     /gene="dnaA"
                     /locus_tag="PK590_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016994935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOG23566.1"
                     /translation="AIAYEISRRFPAFKILYISSEQFTNQLITAIQTRSTQQFRAKYR
                     TLDLLLIDDIHFIAGRDSTQEEFFNTFNSLYDAHKQIIVTSDRPPKEIPGLEERLVSR
                     FGWGLVTDIQPPDFETRVAILKKKIEKETVEVPDEVSYFIAGKIKSNIRELEGALIRV
                     VAYCSLTGAKLDVVLAQDILKDAFKEESQKFSIEEIQKTVADYFQIKVSDLRAKKRTL
                     SIVRPRQIAMFLVRELTHYSLPEIGEYFGGKDHTTVLHACNKITKEKEKDIGLRGILD
                     KIQSLLKKEG"
     assembly_gap    6205..6214
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6215..6697)
                     /locus_tag="PK590_02845"
     CDS             complement(<6215..6697)
                     /locus_tag="PK590_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HOG23567.1"
                     /translation="MDLSKNSVSNPSQSSDLQDLWSRITAILSKELSERSYKTWFDNV
                     SLLRVEGNTLTLTVPDSYYGKWLEDHYQNLILAAAAEVLGRQVTLSYKVVSPQNSVSL
                     NLPAKDKPHNLPSLNSNYTFDNFVIGPGNRFAHAASLAITDAPAKQYNPLFIYGPTGL
                     G"
     gene            7002..7139
                     /gene="rpmH"
                     /locus_tag="PK590_02850"
     CDS             7002..7139
                     /gene="rpmH"
                     /locus_tag="PK590_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003148884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOG23568.1"
                     /translation="MKRTYQPSKRKRRTKHGFLARKRTKNGRKVLARRRKKGRKRLTQ
                     V"
     gene            7141..7515
                     /gene="rnpA"
                     /locus_tag="PK590_02855"
     CDS             7141..7515
                     /gene="rnpA"
                     /locus_tag="PK590_02855"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOG23569.1"
                     /translation="MKHPKRFTSEQRIRGQDAFKNLVEKGRFSKGRFFYLWAGEASLL
                     VRKTSGKPMLGVVVNKKTQASAVKRNLIKRRVREVFRSLQDSIHPETAVLIKAKETST
                     MPGLMEIREDLATLFKKVGISL"
     gene            7512..7724
                     /gene="yidD"
                     /locus_tag="PK590_02860"
     CDS             7512..7724
                     /gene="yidD"
                     /locus_tag="PK590_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015212679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOG23570.1"
                     /translation="MKIIVAFIFQCYRAVISPFFGPACRFTPTCSHYAEEAILKKGLL
                     RGGAASVVRILKCHPFHPGGYDPVKE"
     gene            7728..9362
                     /gene="yidC"
                     /locus_tag="PK590_02865"
     CDS             7728..9362
                     /gene="yidC"
                     /locus_tag="PK590_02865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOG23571.1"
                     /translation="MDKNYLKAIVFSLLILLFYPVLLKWFYPNQAQTARVDAPAPVTD
                     SPIQGEAPPSADENSYLKQAVKPVTVPFENQHYRIEFSTIGGTVTRLFYKGEQGRESL
                     TQTEFFSAPVGEAGIFGMNIAGEVADLTYTPFKLNRRGRAGREVFEFTYELPAQYEVT
                     KTFTPNPDKPALDLSVRIRNLAGEERRFPLTIMYGMNHEKDFQKNYGFFDAVAYNGKI
                     KTANIGKLKKKGFAAPDNLLWAGMIKKYFALLVKPEWKIETGKARADEQVMRGEIGMA
                     SLVIPAGGEDTRHFLIYAGPQRYETLRGFGCDFEKVFSRGFLGNFKTILLAILKFFYG
                     FTHNFGWALVLLTLLIKLVFAPLTHVSYANMKKMQIIQPKMKAIQEHYKNDPQKQQRE
                     LMELYRRNKVNPVMGCLPMLIQIPIFIAMFRLLPEAIELHGAPFVGWITDLSAPDRLI
                     TFRASLPLLGWNSLNILPILMALSQLWYQKIMPQQPGASPEQANIMNFMPLFFGFICY
                     NMPSGLTLYWTLQNLFSIIQQVFINRIAIVLHHEDQ"
     gene            9447..>9772
                     /locus_tag="PK590_02870"
     CDS             9447..>9772
                     /locus_tag="PK590_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG23572.1"
                     /translation="MTSAREFAAIVVGAGHAGVEASLAIARCGFPVLVITLDEKVIAR
                     LSCNPAIGGLAKGHMVREIDALGGEIGCAIDATGIQFRMLNKAKGPAVWAPRAQADKK
                     QYESYM"
BASE COUNT         2512 a         2314 c         2125 g         2811 t
ORIGIN      
        1 ccgtccacca aggcgagcat gattacgcca aaagagcttt gcatttgagt gtgtttatac
       61 aggttgttca ggacaacctg cggtaccgct tcccttttca gttcgatcac cacacgcata
      121 ccatcgcgat cggactcgtc gcgaagatca gaaatcccgt cgatcctctt atcctccacg
      181 agccggacta tcgattcaat caggttagat ttgttaacct ggtaggggat ttccgtaatg
      241 acaatggcgt cttttttgtt ccgccccgtt tcgatcgccg cctttgcgcg catgacaatc
      301 gcgcctcgcc cggtttcgta agccttcttg atcccgtctc gtcccacgat gagtccaccc
      361 gtcgggaaat ccgggcccgt cactattttg aaaagttctt tgtttgtgat gtccgggtta
      421 tcaatcagcg cgacgcacgc gttgaccact tcggtcatgt tgtgcggtgg aatgttggtc
      481 gccattccca cggcaattcc tgaggatcca ttgaccagca aattgggaat tcgcgacgga
      541 agcagtgcgg gctcagaaag ggattcatca aaattgggca caaaatcaac ggtctcttta
      601 tcgatatccg cgaggagttc tccggcggcg ctggcaagcc gcgcttccgt gtaccgcatg
      661 gccgccgcgc tgtccccgtc aatggatccg aagtttccct gtccctgaac cagcggatac
      721 cggagagaaa agtcctgcgc catgcgtacc agggaatcat aaaccgccgt gtccccgtgt
      781 ggatggtatt tgccgagcac ttctccaaca atgcgggcgg atttcttgta cggcttggtg
      841 tgggcgagac ccagctcatt catcgcaaaa aggatccgtc gatgcaccgg cttcaggcca
      901 tcccggacat ccggaagcgc gcgccccaca atgaccgaca tcgagtagtc gatgtaagaa
      961 tccttcatct cctcttcgat cggtctgaga aggatttttt cgttcatcgc atacagattt
     1021 ttttcttccg ccataacttt tctctccatc gccgcgccgc taaaaacccg cggcggtctt
     1081 gtgtcatctt ttttgtgagc tccgcccttc tcgccgggcg ggctttttaa atatcaaggt
     1141 tcctaaccgc tttcgcgtgc cgctcaataa aagccctgcg cggctccacc gcgtctccca
     1201 taaggattga gaagatttcg tccgcttcaa ccgcgtcttc cagagtcact ctgaggatgg
     1261 ttcgtgtttc cgggttcatg gttgtgttcc acaattgttc cggattcatt tctccaagac
     1321 ctttgtatcg ctgcaacgtc atcccctctt tgccccacgt tttaatttta gaaaggacgc
     1381 tgcgaaggtt tggggcttcc tctttaccat ttttttcgaa aactacttca aacaggggct
     1441 ctttgtcagc ttcgaaatct tcaaaattga tgttgaattt ctgaagtttt ttctttgctt
     1501 ttgcaaattc aaccgattca aatatttcga taaggtcgta aacgctttgg aaggaaatgt
     1561 catcttcgtc ttccttcgcg tcttcttctt tcaccttctt tccttttttc acatcctttt
     1621 tctttgcttt ctctccttcc tttgcgtcgt ccttcctgtc ttgctccagt tttttcacat
     1681 attctttttc tatttcttcg gtatatgccg tcgcctcttt ttccgagtaa aggaacacgt
     1741 cttcattttt ttcaaaaagc cggaccacgt gcgtgggaaa tcctttcttt ttatcttggt
     1801 tttccacgaa cttgtgaagc gaaatgcctt ttcgggcgag ggatcgttcg agatcctcca
     1861 tcgcctggac cagatccatc agttcacgaa gatctttccc gctgacgctt cccttgctcc
     1921 cgtgtttttt aagaagggtt ccctgaagac caaaattcag gagaattccg ttcatctgat
     1981 cttcggtttg gatatattgc tcgtccttgc ctctcttgat tttataaagg ggcggttgcg
     2041 cgatataaac atgcccgttt tcgatcagtt tccgcatttt ccggtaaaaa aacgtgagga
     2101 ggagagtccg gatgtggctt ccgtcaacgt cggcgtcgca catcaggata attttgtgat
     2161 accttaattt ttcgatatta aactcatctt cgatgccggt cccgatcgcg gtgataatgg
     2221 tgcgaatttc tttgttagaa agcaccttgt caattcgcgc tttctcgacg ttgataattt
     2281 ttccgcggag aggaaggatg gcctgaaacc gcctgtcgcg tccctgtttt gccgaacctc
     2341 cggccgaatc tccttccaca agatacattt cgctgttcgc gggatcttca tctgaacagt
     2401 cagccagttt ccccggtaag gaagcggact cgagagctcc ctttcggcgc gtcaactccc
     2461 tggctttccg ggccgcttcg cgagccctga gggccagcgc ggccttttca acaatcttgt
     2521 tcgcgaccgg cgggttttct tcgaaaaaag tgctcaggct ctcgtaaaga accgagtaaa
     2581 caatcccctc cacctcactg tttccgagtt ttgttttggt ttgaccctca aattggggac
     2641 gcgggacctt aacgctgata acagccgcca aaccttctgt cagatccgtt ccgctcaatc
     2701 caccctcaag atttttaaga aggtttttgc tcttcgcgta ctgattcgtg gcgcgggtca
     2761 aagaagtttt aaatccgctt aaatgtgtcc cgccctcaat ggtgttgatg ttgttggcaa
     2821 acgaaaaaac atcttctccg tagccgtcgt tgtattggag cgccgcctct acttcgacgc
     2881 catccttggt tttatcaaca taaaaaatct tccggtgaag aggggttttg gaccggttaa
     2941 tgtactggat aaattccgaa atacccccct tgtacataaa gacatgctct ttgatctcct
     3001 ttttgtttcg ctcatcgcgg atcgtgaccc gaacgccctt atttaaataa gcgagttccc
     3061 gcaggcgatt ggaaagcacg tcgaaactaa attccgtttt ttcaaaaatt tccttatcgg
     3121 gaagaaaaac aacggtcgtg ccgtgttctt tggttttccc tttttcaata actttggata
     3181 cggcacgccc tttttcatac cgttgttgcc aaatttttcc atcccggcgt actttgactt
     3241 cacaccagga cgacagggcg ttggtacagg aaactcccac gccatgaagc cctccggaca
     3301 cgcggtaaga cttattatca aatttcccgc cggcgtggag cgtcgtcatc accacttcaa
     3361 gcgccgattt tttctgcgtt ttatgcatat caaccgggat accgcggccg tcatcggtga
     3421 tcgcgcaact tccatccgcg ttaatcacca cgtcgacccg tgacgcaaag cccgccaacg
     3481 cttcgtcaat ggagttatcc accacttcat aaacaagatg atggagtccg cgcaatgttg
     3541 tgtcgccaat gtacatcgct ggacgtttcc gaacagcgtc aaccccctca agcacctgta
     3601 tttttgtggc gtcgtatttc gcgcccgaaa gacctggttt actttggtcg ctttttgtca
     3661 taattttatt tccccactag tacttttaac tctttaaccg cgtcttctcc taaaacaacc
     3721 tgcgttcgtt ttaaaagaga gtgtcggtat ttttgactga tctcatacgc aatcgtcgct
     3781 tcacttacat aaacataaag gatgcctttt tgagttaatt gtggtctggt gtgcggagtg
     3841 agctttcctc cggcaatagc cggccatttt tcaattaaca aggaacgtgt ttttttttca
     3901 gggttctcca acccgcggat aactgacggg ataacgcttc gaatacactc tatttcacac
     3961 cgctttatcg acatttttct cttttaatta agtggccctt caggaagttt tttttcaaaa
     4021 gaaacatctt gagtgggtcg ggaaaatccc ctaacttctt aacttagttg catcggcatg
     4081 atgacataaa gatatccatt ccgtcccttc acgagaccag gtttatctgg ttccgtgaga
     4141 gcgaaggtaa tactttcatc ggtcaggttt tttaaaagat cgatgaaata atttggatta
     4201 aatccaattg ttacttcctg cccgctcacc tcagcgggaa cctcttccct tgcctccccc
     4261 atattagggg attttgcgga tattaaaact tttcctttta taaaatcaaa ttttacagcg
     4321 ggtgattcaa tggaagttaa taacgccacg cgccgtaccg cctgcagaaa ttcttcacaa
     4381 tgaacgaccg cggtagtttt ttcttcttta ggaatgactt gttcataatt agggaaattt
     4441 ccttctatta aactggatgt gagataagta tcctgaaatt taaaaattac cttgttttgg
     4501 gagtgtgata ttttaacttt cccctcccac tcaagtaatt tcagtaattc atgaatggtt
     4561 tttgaaggga taatcatgtc tatcttttta cctatttttt ccttaaggtc ttttttataa
     4621 aaagcaagcc gccttccatc tgtggcgata aaacggattt gattctcctg aagagacact
     4681 aagacaccat ttaaaacata ccgtgtttca tcatgggaaa tcgcgaaaga cgtaagaata
     4741 agactttctt taaggatact ttgctccaac tcaacctctt gttcaggaga cggatcggga
     4801 agttttggaa aatcctcctt atcaaggccc atgattttaa aaaaggattt tccgctcttg
     4861 atattaacgg tattattttt tgtgacagaa agactaatct ccccctcggg aagttctttt
     4921 acaatgtcat gaaattttct cgcgggcact gtaacacttc cttgttgccc tatttccaca
     4981 ggaagtaaag ttgaaactcc cacttccata tctgttgcca caatctttaa cattccatcc
     5041 ttttgagttt ctaataaaag attattaaga atagggagag tgctcggttt ccctccagca
     5101 atacttactc ccaaaccaat tttttttagt aattcatttt ttgggattct gatatccata
     5161 tctaactctc cttcttcttt ctctatgatt ctttaataaa taaaaatagc cgttgtagaa
     5221 ctagtacttt atgattcttt aataaataaa aataaccgtt gtagaactag tacttgttga
     5281 attgtggata tctttctaaa ttattataat aaaacaagat ataaataaaa atcctgtgga
     5341 tatcatcctt ctttttttaa caagctctgt attttatcta atattcctcg taacccaata
     5401 tctttttctt tttcttttgt aattttatta caagcgtgca gtactgtggt gtgatcttta
     5461 cctccaaaat attccccaat ttcaggtaaa gaataatgag tgagttctct tactaaaaac
     5521 atggcgattt gtctcggacg cacaatagag agagttcttt ttttagctcg aagatccgag
     5581 actttaatct ggaagtaatc cgcgacagtt ttctgaattt cctctataga aaacttctgt
     5641 gactcctcct taaaggcatc tttaagaata tcttgagcga gcacgacatc aagttttgcg
     5701 cccgtgaggg aacaataagc caccacccgg attaaagctc cctctaactc acgaatattt
     5761 gatttaattt ttcccgcgat aaaataagaa acttcatcag gtacctccac agtttccttt
     5821 tctatttttt tctttaaaat agcgacgcgt gtctcaaaat cagggggttg aatatcagtc
     5881 actaatcccc aaccaaagcg ggagaccaat ctttcctcta acccgggaat ttctttaggg
     5941 gggcggtcgc tggtgacaat aatttgtttg tgcgcgtcat aaagagaatt aaaagtatta
     6001 aaaaattctt cctgggttga gtcccttcca gcgataaaat gaatatcatc aataaggaga
     6061 aggtcgagcg ttctatattt agctctaaac tgttgtgtgg agcgggtttg tatcgctgtg
     6121 attagctggt ttgtgaattg ttcgctggaa atataaagaa ttttaaaggc tggaaaccgg
     6181 cggctgattt cataggcaat cgctnnnnnn nnnntccaag gcctgtcggg ccataaataa
     6241 ataaaggatt gtattgtttg gcgggagcgt ctgtgatcgc gagagaagcg gcatgagcaa
     6301 accggttccc cgggccaatc acaaaattgt caaaggtata attggaattg agagaaggta
     6361 aattatgagg tttgtcttta gcgggcagat tcaaagaaac agagttttgg ggagatacca
     6421 ccttataaga aagagttacc tgtctgccta gcacctccgc cgccgcggca aggattaagt
     6481 tttggtaatg atcttccagc cattttccat aataactatc aggaactgtg agagttaggg
     6541 tgttaccttc gacacgaaga agcgagacat tatcaaacca tgttttataa gatcgctcac
     6601 ttaactcctt ggacagaatc gccgtgattc gggaccaaag gtcctgtaag tctgaacttt
     6661 gagaagggtt ggaaacagaa ttcttcgaga gatccatagt taaaacttat caacaggtta
     6721 taaagataac aacattataa aaaccgttat ttctaagcga gatacaaaaa gttattaaaa
     6781 aatattcact aaatattaac aggttataaa caacacaaaa aacaaaaaaa taaactcccg
     6841 gaaaaaagaa atacttatta ttccgagaga ggagccgcat tctaccccgc ccgaaaaaaa
     6901 agtccagaac aaaaaaaaga aaaaaaatcc atctctcgaa aaaaaaagta aaaacaataa
     6961 aaagctggat attaatacga aattcaatat aatccgctgt catgaaacgg acctatcagc
     7021 cctccaaaag aaaacgtcgc acgaaacacg gatttctcgc tcgcaagcgg acgaagaacg
     7081 ggcgcaaagt gttagcgcgc cgccgcaaaa aaggcagaaa acgcctgacc caagtctgat
     7141 ttgaaacacc ccaagcgctt tacgtcggag caaagaatcc gcggacagga cgcttttaaa
     7201 aaccttgtgg aaaaaggtag gttttcaaag gggcgttttt tttatctttg ggcgggtgag
     7261 gcaagtctgc ttgtgagaaa aacaagcgga aaacctatgc ttggcgttgt cgtcaataaa
     7321 aaaacacagg cttcggcggt caagagaaac ctgattaagc ggcgtgtgcg ggaagttttt
     7381 agatctctcc aggattcgat ccatcccgaa actgccgttt taatcaaggc caaagaaacc
     7441 tcgacgatgc ccgggctgat ggaaatcagg gaggatctgg caactctctt caaaaaagtt
     7501 ggcatttctt tatgaaaata attgtcgctt tcatttttca atgctatcgc gctgtgatct
     7561 cgccgttttt cggtcccgcc tgccggttca cgcccacctg ctcgcattac gcggaagaag
     7621 ccatcctaaa aaaagggctc ctccggggag gagctgcctc ggtagttcga attttaaaat
     7681 gtcacccctt tcatcccggc gggtatgacc cggttaagga ataagcaatg gacaaaaatt
     7741 atttaaaggc gattgttttt tcattattaa ttcttctttt ttatccggta ttgctgaaat
     7801 ggttttatcc gaatcaggca cagacggcgc gcgtggatgc cccggcgcca gtaacggatt
     7861 cgccgattca gggggaagcg cctccgagcg ccgacgaaaa cagctacctg aaacaagcgg
     7921 ttaagcccgt gacggtgcct tttgaaaacc aacactacag aatagagttt tctaccattg
     7981 gtggaacggt cacaagattg ttttataagg gtgaacaggg acgtgaatcg ttgacgcaga
     8041 cagagttttt tagcgccccg gtgggcgaag cgggaatctt tggcatgaac atagccggag
     8101 aagtagctga cttgacctat acgcctttta aattaaaccg acgaggaaga gcggggcgcg
     8161 aggtttttga gtttacgtat gaattgccgg cgcaatacga agtgacaaaa acatttactc
     8221 cgaatcccga caaacccgcc ctggatcttt cagtccggat cagaaatctg gcgggagaag
     8281 agaggcgatt tccactcaca atcatgtacg ggatgaacca cgaaaaggat tttcagaaaa
     8341 actacggttt ttttgacgcg gtggcctata acggaaaaat taaaacggcc aatatcggaa
     8401 agttaaagaa aaaaggattc gcggcgccgg acaatttgct ctgggcgggg atgatcaaaa
     8461 aatattttgc gcttttagtc aagccggaat ggaaaatcga aacggggaaa gcgagagcgg
     8521 acgaacaagt gatgagagga gaaataggca tggcgtcgct cgtgatccct gccggggggg
     8581 aagacacgag gcattttctt atttacgcgg gaccgcaacg atacgagacg ctgagggggt
     8641 ttggctgtga ttttgaaaag gttttttccc gcggttttct cggtaatttt aaaaccattc
     8701 ttctggcaat cctgaaattt ttctacgggt ttacgcacaa cttcggatgg gcgttggttc
     8761 ttttaactct tttaatcaag cttgtgttcg cgccgctgac gcatgttagc tatgccaaca
     8821 tgaagaagat gcaaatcatt cagccgaaaa tgaaggctat tcaggagcat tacaagaacg
     8881 atcctcaaaa acagcaacgt gaacttatgg agttgtatcg gcgaaacaag gtgaacccgg
     8941 tcatgggttg tctcccgatg ttgatacaga ttccgatttt tatcgccatg tttcgcctgc
     9001 ttccggaagc gatcgagtta catggcgccc cgtttgtcgg atggattacg gacctttccg
     9061 cgcccgaccg actgataaca ttccgggcgt cactacctct tcttggatgg aactctctca
     9121 acatccttcc cattctgatg gccttatcac aactttggta tcagaagatc atgccccaac
     9181 aaccaggcgc ctccccggaa caagccaata tcatgaattt catgcctctc ttttttggtt
     9241 tcatttgcta caacatgcca tccggtctca cgctttactg gaccttgcaa aatttatttt
     9301 cgatcattca gcaagttttt atcaatcgga ttgcgatcgt cctgcatcac gaggaccaat
     9361 aaacttagcc cttcgttcgg ggtttggaag cacagggttt ttatgacagc gcttctctct
     9421 ccggaacggt gactcctaac ccaactatga cttctgcccg agaatttgcc gccattgttg
     9481 ttggcgctgg ccatgccggc gtcgaggcct ccctcgcgat cgcccgatgc ggttttcccg
     9541 tccttgtgat tactctcgat gaaaaagtga tcgcgcggct atcctgcaat cccgcgatag
     9601 ggggtttggc gaaaggacac atggtgcgtg aaatagacgc ccttggagga gaaataggtt
     9661 gtgccatcga tgccacaggg attcaatttc gaatgctcaa caaggcgaaa ggaccggcgg
     9721 tttgggcgcc gcgcgcacaa gccgataaaa aacaatacga aagctacatg tt
//
LOCUS       DAOSIO010000045         9744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_6737_length_9744_cov_4.202498, whole genome shotgun sequence.
ACCESSION   DAOSIO010000045 DAOSIO010000000
VERSION     DAOSIO010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 9744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9744
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6737_length_9744_cov_4.202498"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PK590_02875"
     CDS             <1..429
                     /locus_tag="PK590_02875"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF013626.2"
                     /note="catalyzes the formation of glutamate 5-phosphate
                     from glutamate in proline biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOG23573.1"
                     /translation="PFMIVNGHQLGILRSILEGKDVGTFFAPSREKKDQRHKWIAFSA
                     RRQGKITVDAGAFRALVEGHKSLLPGGIIKMEGCFEAGQVVDLATAEGKVFGRGVIRY
                     NRKDLALIAGKKTSQIEPLLGRKTSTEVIHRNDMVLWSAR"
     gene            426..1700
                     /locus_tag="PK590_02880"
     CDS             426..1700
                     /locus_tag="PK590_02880"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOG23574.1"
                     /translation="MNSFARKPWFHKLNRTVSRTRQASRESMLWTGNLKKRLLLDIAK
                     RIRANLGTILSENLKDVKRARLAGLSAAMIDRLSLNEARLLAMEEGVRGVANLEDPIG
                     KVLSRWTRPNGLRLQKVSVPLGVILIIYESRPNVTVECAALCLKSSNAVILRGGREAM
                     NTNKVFLRIFRESLRQFKLPVDAVNLVETTDYDAVDFLLLQEGRIDLVIPRGGEKLIR
                     RVVERSRIPVVKHYQGVCHVYVDRHADLKKAVAITVNSKLQRPGVCNAMETLLVHKSI
                     ASRFLGQVGKVLNEGGCEVLGDSETCRILPFAARAKPTDYGHEFLDKVLAVKVVKSLK
                     EAIEHIESYGSHHTDAIVTEDRKTANDFKAKVDSSSVMVNASTRFSDGYEYGFGAEIG
                     ISTDKIHARGPMGLDGLTSYKYIVDGNGHIRI"
     gene            1713..2294
                     /gene="nadD"
                     /locus_tag="PK590_02885"
     CDS             1713..2294
                     /gene="nadD"
                     /locus_tag="PK590_02885"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOG23575.1"
                     /translation="MKIGIFGGTFDPIHKGHVAVGLYALKQFRLDRVLFVLSARPPHK
                     QKDKPVASVQHRLAMVRLALRRYTRLKASNLEVNRRGLSFTIDTIKILKKRYPEARFY
                     FILGQDSYEDMKHWREADEIKREVQFLVAGRPGYRIKKQNEPDVSRIRMPLCHAASSM
                     IRKAIAKDPKSVTDLPSCVICYIKRHGLYRGRA"
     gene            2291..3526
                     /locus_tag="PK590_02890"
     CDS             2291..3526
                     /locus_tag="PK590_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOG23576.1"
                     /translation="MNTVLSLSVLGLLFILSSFFSSTETAIFSLSKLDRKKLKEQFPQ
                     ASGWVMNLLEKPHRTLGTILIGNLVVNTSAAAIATLLVVEYLGEEKLGVVMVFFTILF
                     IILCEVAPKIIAVRSKIKISLISAIPLRFFVYIFTPVRLLMRFITEKIVSILVREKVE
                     QSDLISENELKVLVKIGEEEGVLRRQERFMIQKLLDLGERPVKDIMTPRTDVVGLDIA
                     DSEETHAMTIKSNHYCHMPVFQESLDNILGVVTGLEYLLAEPRPQVKDILKKALFVPE
                     SKRIDDLLETFRTQDEQFAVCVDEYGGTAGIVTQEDVLEEIFGEFYDEYANIENPIRQ
                     LGAGAHVVEGKIPLQDFNAYFLSNLESEESTTLGGYLLEKMGEVPARGAKYETEDFEF
                     TVQSMIRQRIQHVLVRKRL"
     gene            3523..4476
                     /locus_tag="PK590_02895"
     CDS             3523..4476
                     /locus_tag="PK590_02895"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOG23577.1"
                     /translation="MISLGILFLLSLVLSSFFSASEMAFVSSNKLEIRELAENNDPRA
                     RRVMELQKNSQNFLASVLTWHNIVNITATSILAYFLERYWQIRNEWVVTAILAPVLII
                     FCEMVPKDYARMRATPFLMKQVFWIQLFVKLFSFPMSLFLGFIRIFWPSFGKRQKEDL
                     FVNEEELRSLIEESTCQGVVSEQEQKLIHTILDFERIQVQSVMIPVEQIPMVNLHSSV
                     SDVKELARESRSRMMLVYEEMPSIVVGMIYVFDLLRKGESAQGLRDFLRAPVFIPGTT
                     SIEKAFFTLQQKRQSYAVVTDSLKNIKGIVPIERLLIFEKR"
     gene            4685..5785
                     /locus_tag="PK590_02900"
     CDS             4685..5785
                     /locus_tag="PK590_02900"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG23578.1"
                     /translation="MRVIAIANQKGGCGKTTTSINFSACLHFLQKKVLLVDLDPQGHS
                     TCGLGIKTLKGTPTIYELLSPIQAIPTSFTQVIRTLYPDFDIMPSQASLNALEEEANF
                     MPNHHLLLKNLFARMEEQHRGYDFVILDCPPNLGILTWNALNAADEVIIPVEPSFFSL
                     HGLGKISETVQNLDRTRQTPLTVHALLTNFNSLLSFSQEIHDDVRAHFKEQLFRTIIH
                     ENVMLKEAAAAGQSIAQYAPGSPAFQDYLNLAVEYLERDWDRKLPSETLGWDNVYKNR
                     FGPKLMSDGILFQFQSKTASSVEVAGDFNRWVPESLVRRNDDGLWQKVIRVGEKAFRY
                     KFIVDGEWQVDPSQPTQKENAYGGLDSYAEFA"
     gene            5785..6594
                     /locus_tag="PK590_02905"
     CDS             5785..6594
                     /locus_tag="PK590_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23579.1"
                     /translation="MEHSKKLVRGLKDISPIFSAPGRPEVREIPDIQILSVSSPHDDG
                     DSLLLNTYFASQLASSEKPCSLVSILSRTANRCAWRSDAQKIEPFGQNIKRHSLFWDE
                     LKEILDRGLREQESCLGSRNIFMDFEYRSLLHLPKVLELLDQWVLLLKPTPESLTEGY
                     KMIKTGLALNPHLECFILLHAKANPPADSTIFEKFSAVASKHLRIHLGWLGWLDLSDP
                     EHFFQAKINVEQLCFKPARMRLSPERLTLSRWVESFEKNPAVGMEDLEEQG"
     gene            6591..7127
                     /locus_tag="PK590_02910"
     CDS             6591..7127
                     /locus_tag="PK590_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23580.1"
                     /translation="MISLNEITGQQRVFDAVRTTVQELHADCSFVEDVTSSPLLFKSE
                     DEKIWVRIFYQPMNGSSAGEIAREVMKLKLLMPKHAVIYFFYPELDRDQIVSLRSRYR
                     HIAFFEYGSLLETANGKEGVKVCKWIPISELSPAGTVEIKSPFFGESKPHPFLSHARL
                     TQDEISELNDIGLSFRDV"
     gene            7446..7883
                     /gene="mraZ"
                     /locus_tag="PK590_02915"
     CDS             7446..7883
                     /gene="mraZ"
                     /locus_tag="PK590_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003386267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOG23581.1"
                     /translation="MFYGEYEHVIDNKGRVMIPAKFREIFRERYVEKFFLTRGLDQCL
                     FVFTEEQWKAEEKKFREMPYIKAESRTFNRLLFSSACEVVCDKQGRILIPDYLKNYAE
                     VHVDVIIVGVSNRIEIWAKEKWTQFFDANKNRYEELAEKLISE"
     gene            7900..8238
                     /locus_tag="PK590_02920"
     CDS             7900..8238
                     /locus_tag="PK590_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG23582.1"
                     /translation="MAYHSKARLSVEVLRFDGDLSVLSMIKMKGRINRLLNRNQRRLL
                     LDLSETRRVELAGVGILVDRLRKVREQKGDVKLCNLRPEVEKTLRLIGVNDLMESYTT
                     QEEAIRSFDS"
     gene            8228..9136
                     /gene="rsmH"
                     /locus_tag="PK590_02925"
     CDS             8228..9136
                     /gene="rsmH"
                     /locus_tag="PK590_02925"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019588234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOG23583.1"
                     /translation="MIHKPVLLQEIKEFLNVPPGAVVVDGTLGSGGHAQAFLECLGAT
                     GRLIGLDQDPEAITRCRRKFDGDDRVILVHRNFREIKEVLKDLNLMKVDAVLLDIGMS
                     SDQLANEKRGFSFQREGDLDMRMNTAEGEKASYYLQTLPEKEIARVLYEFGEERQSRR
                     IAAAIVRERRLAPIQTTQRLCEVVENSFPRARQFEKGKRPVWNRRHPATKVFQALRIL
                     VNDELGALREGIDGAMSCLKPGGKLGVISFHSIEDRIVKEKFRNFVREGSGKLVNRKP
                     LVAKRSEVIDNPRSRSAKLRVIEVVS"
     gene            9133..9525
                     /locus_tag="PK590_02930"
     CDS             9133..9525
                     /locus_tag="PK590_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23584.1"
                     /translation="MKGSGKILIGIASVTCLMLMYVHLQVASVIVSFDLYERSNVFAQ
                     KQELLRRLQFNVDQLKAPRLLEEKVKKYQLSLALPNKVESCEVPMVPELFGPSPRETH
                     TISATPSVFSRFFGHLIQTAQAKTDFSS"
     gene            9529..>9744
                     /locus_tag="PK590_02935"
     CDS             9529..>9744
                     /locus_tag="PK590_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23585.1"
                     /translation="MRARFSQQRFWVLYGALFILFGLIIYQVVQLTYFHQPSLLELAH
                     RQHYVTVDIPPMRGPITDRINREFVTNL"
BASE COUNT         2412 a         2183 c         2567 g         2582 t
ORIGIN      
        1 ccgtttatga ttgtgaacgg acaccaactg ggcattcttc gttcgatcct cgagggaaaa
       61 gacgttggga cattttttgc gccctcccgc gagaaaaaag accaacggca caagtggatt
      121 gcgttttcgg cccggcggca gggcaaaata accgtggatg ctggtgcgtt tcgtgcgctt
      181 gttgaagggc acaaaagcct tttgccggga ggtattatca aaatggaagg ttgctttgag
      241 gcaggtcagg tggtggacct ggcgaccgcg gaaggaaaag tttttggacg cggggtgatc
      301 cggtataacc gtaaagatct tgccttgatt gccgggaaaa agacttccca aatagagccg
      361 ctgcttggcc gaaaaacttc taccgaagtg atccatcgaa acgacatggt tctttggagc
      421 gcccgatgaa ttcctttgct cggaagccat ggtttcataa gctaaaccgc acggtttccc
      481 gcactcgtca agcgtcccgt gaatcaatgc tctggacggg gaatcttaag aaaagacttc
      541 ttcttgacat cgccaaacgg attcgggcga atcttgggac gattctttct gaaaatctga
      601 aagacgtcaa acgcgcccgt ttggcgggcc tttctgcggc gatgattgac cggctttcgc
      661 tgaatgaagc gagacttctg gcaatggaag aaggagttcg cggggtggcg aaccttgagg
      721 atccgatcgg gaaagtattg tcccggtgga cccggccgaa cggattaaga ctccagaaag
      781 tgtccgtgcc tcttggggtc attttgatta tctatgaatc acgcccgaat gtcacagtgg
      841 agtgtgccgc tttgtgcctg aaaagttcta atgcggtaat ccttcgcggc ggccgcgagg
      901 ccatgaatac caacaaagtt tttttaagaa tctttcgcga atctctccgc caattcaagt
      961 tacccgtgga cgccgtcaac cttgttgaaa ccaccgacta tgatgccgtt gatttcctgc
     1021 tgttgcagga aggacgtatt gaccttgtga ttccccgcgg aggggaaaag ttgattcgac
     1081 gggtggtaga acggtcccga atacccgtgg tgaaacatta ccagggggtt tgtcatgttt
     1141 atgtggaccg gcatgctgat ctcaaaaagg ctgtcgcgat caccgtcaac tcgaagttgc
     1201 agcggccggg ggtctgtaac gcgatggaga ctttgctggt gcataagtca atcgcttccc
     1261 ggtttctcgg tcaagtgggc aaggtcctga atgagggggg atgtgaagtc ctcggagatt
     1321 cagaaacctg ccggattttg ccctttgccg cgcgcgcgaa accgacggat tatggccatg
     1381 aatttctcga caaggttctt gccgtcaagg tcgttaagag tctgaaagag gcgattgaac
     1441 acatcgaatc gtacgggtca catcacacgg atgcgattgt gaccgaagat agaaaaacgg
     1501 caaatgattt caaggcgaag gtcgactcct cttcggtcat ggtgaatgcc tcgacccggt
     1561 tttcggacgg ttatgaatat ggttttggag cggagattgg catttcgact gacaagatac
     1621 acgcccgcgg ccccatgggg ctggatgggc ttacctctta caaatatatc gtggatggaa
     1681 atgggcatat ccgtatttag acggtcacca gcatgaaaat aggtatcttc ggaggcacgt
     1741 ttgatcccat tcataagggc cacgtcgccg tgggactgta tgcgctgaaa cagttccgtc
     1801 ttgatagagt tctctttgtt ctttccgccc gccccccgca caaacaaaaa gataaacccg
     1861 tcgcctccgt tcaacatcgg ctcgccatgg taaggctggc cttgaggaga tacacgagat
     1921 tgaaggcatc gaatctcgaa gtgaatcgtc gcgggctatc tttcaccatt gataccataa
     1981 aaattttaaa aaaaagatac cccgaggcgc ggttttattt tattttaggg caggattctt
     2041 acgaggacat gaaacattgg cgagaagcgg atgaaattaa acgggaagtt cagttccttg
     2101 tggcgggtcg cccgggatat cgcatcaaaa aacaaaatga gcctgacgtt tcccgaatcc
     2161 gtatgccgct ctgtcacgcc gcgtcgtcga tgattcgtaa ggcaatcgcc aaggatccaa
     2221 aatcggtgac ggaccttccg tcttgcgtca tttgttatat taaacgtcac ggactttaca
     2281 ggggtcgggc atgaatacgg ttctcagtct cagtgttttg ggactgcttt ttattttatc
     2341 gagttttttt tcttcgacgg aaacggcgat tttttcactt tccaaattag atcgcaagaa
     2401 actcaaagaa cagtttccgc aagcctcggg atgggtcatg aatcttcttg aaaagcccca
     2461 tcgaactctc gggactattt tgatcgggaa cctcgttgtc aatacgtcag cggcggcgat
     2521 agcgacgctt ctggtcgtgg agtatctggg cgaggaaaag ctgggtgtgg tgatggtctt
     2581 tttcacgatt ctctttatca tcctctgcga agtcgctccc aaaattattg cggtcagaag
     2641 caagattaaa atttccctga tttcggcaat tccgctccgt ttttttgttt atatttttac
     2701 gcccgtccgg cttttgatgc gttttatcac ggaaaaaatt gtttcgatcc tcgtgaggga
     2761 aaaagtcgaa cagtccgacc tgatttctga aaatgaactg aaagtgttgg tgaagatcgg
     2821 ggaagaggaa ggcgtgttga ggaggcagga gcgtttcatg atccaaaagc ttctcgacct
     2881 tggcgagcga ccggtgaaag atattatgac tcctaggacc gatgtcgtcg gtctggatat
     2941 tgcggatagc gaggaaacac atgccatgac gatcaaaagc aatcattatt gtcatatgcc
     3001 ggtttttcag gaatccctgg ataatattct tggagttgtg acgggtctgg aatatttgtt
     3061 ggctgagccg aggccgcaag tgaaggatat tttgaagaaa gcccttttcg ttcccgaaag
     3121 taaacgtatc gacgaccttc ttgaaacgtt cagaacgcaa gacgaacaat ttgccgtctg
     3181 cgtcgacgaa tatggtggaa cagcggggat tgtaacacag gaggatgtac tcgaagagat
     3241 ctttggagag ttttatgacg agtacgcgaa cattgagaat ccgatccgtc agctgggggc
     3301 gggggcccat gtggtcgaag ggaaaattcc gctccaggat tttaacgcct attttctctc
     3361 caatcttgag tctgaagaat ccacgacgtt gggagggtat cttctggaaa aaatgggaga
     3421 ggtcccggcg cgaggcgcca aatatgaaac ggaggatttt gagtttaccg ttcaatcgat
     3481 gattcggcag agaattcagc atgttcttgt gaggaaacgc ttatgatctc gctcgggata
     3541 ctctttctgc tttcgctcgt gttgtcttcc tttttttccg cctctgaaat ggcttttgtg
     3601 tcttcgaata aactggagat tcgcgaatta gcggaaaata atgacccccg ggcccgacga
     3661 gtgatggaac tccagaaaaa tagtcaaaat tttcttgcct ctgttttaac gtggcacaac
     3721 atcgttaata ttaccgccac atccattttg gcttattttt tagagcgcta ctggcagatc
     3781 cggaatgagt gggttgtgac ggcgatcctg gcgccggtgc tgatcatctt ctgtgaaatg
     3841 gtacccaagg actacgccag aatgcgggcg acgccatttc ttatgaagca ggtgttttgg
     3901 attcagcttt tcgtgaaact tttttctttt cctatgtctc tgtttttggg tttcattcga
     3961 attttttggc cttcgttcgg caaacggcaa aaagaagatt tattcgtcaa tgaagaggag
     4021 cttcgctccc tgatcgaaga aagcacttgt cagggggtgg tgagcgagca ggagcagaag
     4081 ttgattcaca ccatattgga ttttgaaaga atacaagtcc aatcagtgat gatccctgtg
     4141 gagcagattc ccatggtgaa ccttcatagc agcgtaagtg acgttaaaga gttggcccgc
     4201 gagagccggt cccggatgat gctcgtgtat gaagaaatgc cttctatcgt ggttggcatg
     4261 atctacgttt ttgacctgct ccggaaaggg gagagcgccc agggactgcg cgattttctg
     4321 agagcccccg tttttattcc gggaaccact tccatcgaaa aggctttttt caccctacaa
     4381 caaaagcgtc aatcttacgc ggtcgtgacg gattctctca aaaacatcaa agggattgtt
     4441 ccgattgaac ggcttctgat tttcgagaaa cgctgacgtg ctgtcgtttt tcgcgactta
     4501 tttgtcttaa aactcctgac tgttcctcca cgtaaatctt cttacggtcg tatgccccct
     4561 cctcggtcgc ttttcttggt ttggcgaacg ggtcactctc gaaacaaatc aaatctatca
     4621 agctttaagg agtaaagccg ttaaataaat atctttttag tacgctgata aggagaatcc
     4681 acccttgaga gtcatcgcga ttgcgaacca aaaaggggga tgtggcaaga cgactacaag
     4741 catcaacttc tcggcctgcc tgcatttttt gcaaaagaaa gtgttgcttg tggatcttga
     4801 tccccaagga cattcaacct gtggtcttgg aatcaagacc ttgaagggaa cgccaacaat
     4861 ttatgaattg ttgagcccca ttcaagccat cccgacttca ttcacacagg tcatacgaac
     4921 gctctatcct gattttgata tcatgccctc ccaggcttcc cttaacgcgc ttgaagaaga
     4981 agctaatttc atgcccaacc atcatcttct actgaaaaat ctttttgcca ggatggagga
     5041 gcaacatcgt ggatatgatt ttgtgatcct ggattgtccg cccaaccttg ggatcctcac
     5101 ctggaacgcc ttaaatgccg cggatgaagt gattatcccg gtcgagccat cgttcttttc
     5161 gcttcatggg ctcggcaaga tttctgaaac agtgcaaaat ctggaccgga cacggcaaac
     5221 tcctctgacg gttcatgcgc tacttaccaa ctttaattcg ctcctgagct tttcacagga
     5281 gattcatgat gacgtccggg ctcatttcaa ggaacagctt tttcgtacca tcattcatga
     5341 gaacgttatg ctgaaagaag ccgccgcagc cgggcaaagt attgcgcagt atgctccggg
     5401 gtctccggca tttcaggact acttgaatct cgcggtggaa tatcttgagc gtgactggga
     5461 tcgcaagctt ccgagcgaaa cgctcggctg ggacaatgtg tacaaaaacc gttttggccc
     5521 caaacttatg tcggacggaa tcttgttcca gtttcaaagt aaaacggcat cttccgttga
     5581 ggtggcggga gatttcaacc gttgggtccc cgaatcgctt gtccggagaa atgacgacgg
     5641 gctttggcag aaggttattc gggtggggga aaaagcgttt cgttacaagt tcattgtgga
     5701 tggagaatgg caggttgatc cgagtcaacc aacccaaaaa gagaatgctt atggcggcct
     5761 tgattcttac gcggagtttg cctgatggaa cactctaaaa agctggtacg cggcttaaaa
     5821 gatatttccc cgattttttc ggctccggga cgtcccgagg tcagggaaat ccccgatatt
     5881 cagatcctca gcgtttcaag cccccatgac gatggcgatt ctctgctttt gaacacctac
     5941 tttgcttctc agcttgcctc ttctgaaaag ccctgctcgc ttgtttcaat cctgtctcga
     6001 acggcgaatc gctgtgcctg gcgttcggat gcccaaaaaa tagaaccttt tggccaaaat
     6061 attaagcggc attctctttt ttgggatgag ctcaaggaaa ttcttgaccg gggattgcgg
     6121 gagcaggaat cttgtttggg aagccggaat atttttatgg atttcgagta tcgaagcctc
     6181 ttgcatcttc cgaaggttct ggagcttttg gatcaatggg ttttgctcct gaagcctacg
     6241 ccggaatcgt tgacggaagg ttataaaatg attaagacgg gattggccct caatcctcac
     6301 ctggaatgtt tcattttgct tcacgcgaaa gcaaacccgc cggctgacag cacgattttc
     6361 gaaaaatttt cagcggttgc ttccaagcat ttgcgaatcc atcttggatg gctgggctgg
     6421 cttgatttaa gcgatcccga acattttttt caggccaaaa tcaatgtgga acaactttgt
     6481 tttaaaccgg ctcggatgag actgtcgccg gagcgattga ccctttcccg ctgggtggag
     6541 tctttcgaaa aaaatccggc tgtcgggatg gaggatttgg aggaacaggg atgatttctt
     6601 tgaatgagat tacgggacaa cagcgagtgt ttgatgcggt tcgaacgacg gttcaggagc
     6661 tgcacgccga ttgttctttc gttgaggatg tgacgagttc gcctctgctt tttaaaagcg
     6721 aagatgaaaa aatatgggtc agaatttttt atcagcccat gaacggttct tcggccgggg
     6781 agattgctcg tgaggtcatg aaattaaagt tgctgatgcc gaagcacgcg gtgatttact
     6841 ttttttatcc cgaacttgac cgtgatcaga ttgtttcctt gcgctcgcgt tatcgccaca
     6901 ttgccttttt tgagtacggg agccttttag aaacagcgaa cgggaaagaa ggggtgaaag
     6961 tttgcaagtg gattcccatt tctgaattgt cgccagccgg tacggttgaa ataaaatcac
     7021 ctttctttgg ggaaagcaaa cctcacccgt tcctaagtca cgcgcgactt acccaggatg
     7081 aaatctctga actcaatgat attggtttga gttttaggga tgtttagttg tttcaagata
     7141 caggtcaaat tcccataact atctcgtagc cccctgcttt gaacaggcgt tctcttctcc
     7201 gccgatagcc ccttccgaac caaagacttc cttatgaatg agagggcggg gagtttcctc
     7261 accccactca tccttcgact tcgctcccaa cgggtctgaa gacgggatga tgccctcgga
     7321 gaaaacaccc ccgagggggg ggaattaaat aatattgaca cactattaag gtcggactat
     7381 acttgcgccg ttaataaagt ggtagaaagt ggtagaaagt gggagacaga gaggcgagat
     7441 agaaaatgtt ctacggcgaa tatgagcatg tgatcgataa caaaggacgc gtgatgattc
     7501 ccgcgaagtt ccgcgagatt ttcagggaac ggtatgtgga aaaattcttc ctgacgcgtg
     7561 gtctcgatca atgtttattt gttttcacgg aagaacaatg gaaggcggaa gaaaagaaat
     7621 ttcgcgagat gccttacatc aaggccgaat cgagaacgtt taaccgtctt cttttttcaa
     7681 gcgcttgtga agtggtgtgt gacaaacagg gaagaatttt gattccggat tatttgaaaa
     7741 actacgccga ggttcatgtg gacgtcatca ttgtgggcgt gtccaaccgc attgaaatct
     7801 gggcgaaaga aaaatggact caattttttg acgcgaacaa aaaccgttac gaagagttag
     7861 ctgagaagct gatttccgag taagggggag gaggttttta tggcttatca ttcaaaggca
     7921 aggttaagcg tggaggtgtt gcgctttgac ggtgatctca gtgttttgag catgattaaa
     7981 atgaaaggac gcatcaatcg tttattgaat cggaaccagc gaagattact gctggacctt
     8041 agcgaaacgc ggcgcgtgga gctcgcgggg gttggaattc ttgtggaccg cctgcggaag
     8101 gttcgtgaac aaaaagggga cgtcaagctt tgtaacttac gacctgaagt ggaaaagacg
     8161 ttacgtctga tcggcgttaa cgatttgatg gaatcttata ccactcaaga agaggcgata
     8221 cggagttttg attcataaac cggttctttt acaggaaatt aaagagtttt tgaatgtccc
     8281 gcccggcgcc gtggtagtcg atggtacgtt ggggagcggg gggcacgccc aggcctttct
     8341 cgagtgccta ggagcgacag gccggctgat cgggctggat caggatccgg aagcgattac
     8401 gcgatgtcga aggaagtttg acggggacga ccgggttatt ttggtgcacc ggaattttcg
     8461 ggaaataaaa gaggtcctta aggatttaaa tctgatgaaa gttgacgcgg tgcttcttga
     8521 cattggaatg tcatcggatc agttggcaaa tgaaaaacgc ggtttcagtt tccaaaggga
     8581 aggggatctg gacatgcgaa tgaatacagc cgagggtgaa aaagcatctt actatctcca
     8641 gacattgcct gaaaaagaaa tagcccgcgt tctctatgag tttggcgaag aaaggcagtc
     8701 gcggcgcatc gccgcggcca ttgtccgtga acgccgtcta gcccccattc agacgaccca
     8761 gcgactttgc gaagtcgtag agaattcttt ccctcgggct cgccagtttg aaaaagggaa
     8821 acgtcccgtc tggaaccgtc gtcatcccgc gaccaaagta ttccaagcgc tacgcatcct
     8881 tgttaacgat gagctcggtg ctttgcgtga aggaattgac ggggccatga gttgcttgaa
     8941 gccgggcggc aaactgggcg tgatcagttt ccattccatt gaagaccgga tcgtgaaaga
     9001 aaaatttcgt aattttgtgc gggaaggatc cggaaagctg gtgaatcgca agccgttagt
     9061 cgcaaaacgc tcggaagtta ttgataaccc acgctcgcgc agcgcgaaac tcagagtgat
     9121 cgaggttgtg tcatgaaagg tagcggaaaa attttgatag ggatcgcgag tgtgacctgc
     9181 ttgatgctga tgtatgtgca tttgcaggtc gcttcggtga ttgtttcgtt tgacctttat
     9241 gaacgatcga atgtctttgc gcaaaaacag gaattgttgc gccgacttca gtttaatgtg
     9301 gatcagctta aggcgccgag attgcttgaa gaaaaagtaa aaaaatatca actctctctc
     9361 gccttgccca acaaggtcga atcctgcgaa gtcccgatgg tgccggagct ctttgggccg
     9421 tccccgcgag aaactcacac catttcggcc actccgtctg tcttttcgcg attttttggc
     9481 catctcatcc agaccgcgca ggccaagaca gatttttcct cctgacccat gcgggcgcgg
     9541 ttttcgcaac agaggttttg ggtcctctac ggcgctcttt ttattctttt cgggctcatc
     9601 atctaccagg ttgttcagct gacttatttt catcaaccct cgcttcttga acttgcgcac
     9661 cggcagcact atgtcaccgt agacatcccc ccgatgcggg gtcccatcac ggaccgaatt
     9721 aaccgggagt tcgtcactaa cctc
//
LOCUS       DAOSIO010000046         9510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_6925_length_9510_cov_4.160550, whole genome shotgun sequence.
ACCESSION   DAOSIO010000046 DAOSIO010000000
VERSION     DAOSIO010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 9510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9510
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6925_length_9510_cov_4.160550"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5214)
                     /locus_tag="PK590_02940"
     CDS             complement(<1..5214)
                     /locus_tag="PK590_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23586.1"
                     /translation="MTPKISGFFPNVRVTALATVVFFLVTTTGWAAPAIRLSADPRAE
                     FPAQSLADLIEIPSSLGQVTTRYVPRNVPASDRPESSPVTVIHIQDPHGQYEAQKNIR
                     AILEHLENTYQVQTLFLEGGSGRLEDANLKVFKDPGLNREMVDILAHEGFAGGPEMFL
                     AERENGKTARTFSAYGVEDATLYLKNLETFRHVANHSGPTEKFFGRIRVRIKSLASRK
                     FNPELKRYFQTWVFCEERPQEWLNRWEILKDYAVRHLALDLEDPYQQMDWPQAVRFTK
                     LFGLSGSLKQQKSADALPVEWSALKTWLESKGLRDLAAQTKSCLEGKKLPLEFRSVRE
                     FFERFYAEASSRGFDFRTYPNLKMKMGERILTDEIHAPDLITEVERIHERILKKLAAT
                     DLERDLIRLSREARLSEKLLGLQLVRADFREIQTAASSIAPGVIASRLDQLENRSRDP
                     KLPDLDAVYEEALRFYEDVAKRDEAMLENMTETIRQRKISRAVLVTGGFHAEGIKKGL
                     KEKGISFVTLAPKMSEIRDSKTYLDALKGTVDYLTLRSYLRPVTPVTPAGIKELFGNC
                     AAGFHLASATVLYGKPKEVRDGARRSLARMELRYVPLARDGDEAFFYGSRPVSAGEGG
                     IPGLKDGRLALFPASQVRSEARNVPVDLFEKDRRVTDPRMSKKVWDAVGYKLGAKSIT
                     GLEIRAQQNEIPDEPLQWDDEAGRITFFSRKKILNAIPDFKKALTPFFLESIADVAKK
                     RSGGFRAFVRAGVAAGLLGVLGGAGYYGIWFVTRRDVQKFQNSLRDYYQESLKRAYVV
                     EPMRVIGAKRFDVNFKGWETVLIGTAHGAPRAAELLLDPSLTRSLFKNPEEWVIFFEG
                     HKIMSAVPEIPLIDVLMVYKLRDLTGGTIPIIDPLPTQGDSEVMRRTAESLGISLEEI
                     SAVILFQDLFFQLSRIVSRGGPAEAAISNVIKSRTLTMGLPEESVRRILLQYAQKIAG
                     SEEAMNAFVRTGNQVSRTVALLRNEIGKDRLAMELAKYPGKKRLLAVVGVQHVAMFDV
                     PAVHVPPGFRAESYEPMSVRQMQWVLQHLGSLGFQHQRSSPAERGIMSLLDSLEKELL
                     RDDRLREKWEGTRQSVIEELKQQGGATRPEMRMSNSETKGSVEANDRPETRAPEPAIP
                     GADEGARSEVRTSEVNRLRILFESAEKLADEDIDQYVAETEELLGELFDDLFMRLIKA
                     RSQGLLKAISIRLEATNLSALYLKKIWLWSVIAQADAEKINVPDIVKRADSLMEQIRQ
                     RLYVMEMSRSELRRDDETDVTLSGTQMGYFEAPVKEVAAQGRDLIRDLIKKMNTHAPV
                     FKLKYDKSRDRFEVAVNQRRMNLSGVAYEYDGYYNPFYDVTFAADGKQENGLLEEVRR
                     AIDRALNEGQREPDASDLEAWSPTIAPEEIDFSEKPAPGLSPEEMRNYKARLNHDEKR
                     RINFLINDDYSRALANLGKLPGQTKMLSMEWLLGDEALSDFVQHAKHLMYEGESYFLS
                     QVSLSEYIRGYFRDILAFHESPTPELRKKLIERYKRFALSELIKEHPRRKLALLHGES
                     ASSWRVVVEGQDGDAEEDSVVIEPVVYQLKQEMVVWDAANRREKVLPGEKIVFARWKG
                     QRFLFYTSITHGIQNGPIRAWRMGNSYVSYSGWIVKETPENLAELPVALSKALDDVLA
                     ANPGIARAGESAFIKRAEELGFSDFWDYPLLEEIQELRYPADIHAFVTKLTAFTGRNF
                     QIREALEKVREK"
     gene            complement(5520..6698)
                     /locus_tag="PK590_02945"
     CDS             complement(5520..6698)
                     /locus_tag="PK590_02945"
                     /inference="COORDINATES: protein motif:HMM:NF026247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HOG23587.1"
                     /translation="MIQSLTIYTLLLGIMMLCFYRAGCQTKALDYRVLDYYSTTSTPS
                     FWSFSVIFPMLLFAVVFGMRYDVGVDHLAYLDAYLSNRDASKNELLFRVITDFSHSLN
                     LNFVIYFAILAFIQVAFFFYAFKKEIYLFPLLIFFLFTNGDIIFWMNVIRQSIATCIW
                     VCSLNFIVKKKFWRYVMFGVMAFLFHRSAIALFVFYPILVGGRDYLKIIPLQLVLFAS
                     AFVVKEVFFDVIMNFSAVVDFYISLLGAEMYEASYNIDALVENFKESTGTGLAYLWKV
                     AINVIVIIYSGRLKKYYPSRWFNMIYFLYFVGLITYYMFPVGAISFSRPFRYLYIFQS
                     IMLSYFVYYLYKNKTVNNAILAGLIILSFLGIFYLHHITSNRDSHLWYQFYFQSEINA
                     "
     gene            complement(6695..7600)
                     /locus_tag="PK590_02950"
     CDS             complement(6695..7600)
                     /locus_tag="PK590_02950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG23588.1"
                     /translation="MIYFSVIIPHFTKSDTLLLERAVASVPDREDIEVLVVDNSPNKI
                     DRHLFNHRSNVRILFSDNTKGCGHARNVGMEQAKGVWMLFLDADDFFTERAFQVFDSY
                     RDAKFDVIFFKMTSCYSDTLKPAHRHELYIEKIDKFLKDGDEYALRYRWSPAWSRMVK
                     AILVKTNDMWWEEVPVSEDSVFSAKLGIVAKSIYADAAEVYCVTVTQGSITNTRSFKN
                     IESMFDIRIRKNNLLRKHGLNRNTSVMYLIIQSLRFGIRPFLKLFYKALITGNLFVGC
                     QNWFRTTLKISRQALNERRLNPRIK"
     gene            complement(7656..8156)
                     /locus_tag="PK590_02955"
     CDS             complement(7656..8156)
                     /locus_tag="PK590_02955"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOG23589.1"
                     /translation="MKYILDGYNVIHKVPRLAGLLSRSLQEAREGLVRLMSEWRQSRG
                     AEVVIVFDRNKGSLDGVPVRSSRKVNGVTCVFSAPGIEADDEIIAMIRREKVPANVRV
                     VTGDGQILNHCKALGVRTELPSRMFDQTPPKTGKTVASGNSKQLSPARENDITRWYKK
                     QIGLCA"
     gene            complement(8204..9268)
                     /locus_tag="PK590_02960"
     CDS             complement(8204..9268)
                     /locus_tag="PK590_02960"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HOG23590.1"
                     /translation="MANLPAPASYTKLLTDISALCGSARKAIVEAYWRIGQCIVQVEQ
                     KGEIRSAYGSGLITQLSEDLTRRLGPGFSPNQLLDMRRFYLAYSNSRPAVELGWSQHV
                     ELLRVGNLKQRKALVRRVIRERLKRDQLRRLVRQEMVREQVAGNLASKTSGNADVSAP
                     RPAELLVPRRGILYAYRILDPKTFQSGEANLRFLDLGFACYRDLDAVTGRTFTSGEIV
                     QSVRTGEDQYQLDKLPGAEPASLYTYQATVEKVVDGDTLRVIVDLGFNTRTRQYLRLR
                     GVDCPELATEEGKQASTFVKSRLRGADQILLTSSRSDKYDRYLADVYFADREGGEIFL
                     NNLLLEKGLAVRSRTSGEGS"
BASE COUNT         2466 a         2613 c         2115 g         2316 t
ORIGIN      
        1 cttctcccga actttttcga gagcctcacg aatttgaaaa ttacggcccg taaaagcggt
       61 gagctttgtg acaaacgcgt ggatatccgc gggatatcta agctcctgga tttcttccag
      121 taacggatag tcccaaaaat cggaaaaacc caactcttcc gcccgtttga taaaagcgga
      181 ttcaccggcc cgcgcaattc cgggatttgc cgccaaaaca tcatcgagag ctttcgataa
      241 agcgaccggt aattccgcga gattttccgg ggtctctttg acgatccacc cggaatacga
      301 cacatagcta tttcccattc tccaggcccg gatggggccg ttttgaatcc cgtgggttat
      361 tgatgtgtaa aaaaggaaac gttgcccctt ccaccgggca aacacgattt tctctcccgg
      421 caaaaccttt tcacggcgat tcgcggcatc ccaaacgacc atttcctgtt tcaattgata
      481 aacaaccggt tctatcacga ccgaatcttc ttccgcgtcc ccatcctgtc cctcaacaac
      541 caccctccag gaactcgccg attcgccgtg aagaagcgcc aatttccggc gaggatgttc
      601 tttgattaat tctgaaaggg cgaagcgttt gtaacgctca atcagttttt tgcgaagttc
      661 cggcgtcgga gattcatgga aggcgaggat atccctgaaa tatccccgaa tgtattcgct
      721 gagcgacacc tggctcaaaa aataactttc cccctcgtac atgagatgct tcgcgtgctg
      781 gacaaaatcc gaaagagcct catctcccag cagccactcc atggataaca tttttgtttg
      841 tcccggcaat tttcccaggt ttgccagcgc ccgggaataa tcgtcattga tcaggaaatt
      901 gatccggcgt ttttcatcgt gattaagtct ggctttataa ttcctcattt cctcgggtga
      961 caggcccgga gccggtttct cggaaaaatc gatttcttcc ggagcaatag tcgggctcca
     1021 tgcttccagg tccgaagcgt ccggttcacg ttgcccttca ttgagcgccc gatcaatagc
     1081 gcggcgaact tcttccaaaa ggccattctc ctgttttccg tccgcggcga atgttacatc
     1141 gtaaaatggg ttatagtatc cgtcatattc gtacgcgacc ccgctcaaat tcattcttct
     1201 ctggttcacc gcaacttcaa aacggtcccg ggacttgtca tacttgagct taaagacggg
     1261 cgcatgggta ttcattttct ttatcaagtc cctgatcaag tccctgccct gggcagcaac
     1321 ctcttttaca ggagcttcaa agtaccccat ctgcgtaccg gacaaagtca catccgtctc
     1381 atcatcgcgc cttaactcag accgggacat ttccattaca tacaatctct gtctgatttg
     1441 ttccattaaa gaatccgctc gcttaacaat gtccggaaca ttgatctttt cggcgtccgc
     1501 ttgggcaatc acgctccaaa gccatatttt tttgagatat aacgcagaca gattggtggc
     1561 ctccaggcgg attgaaatag ctttcagtag cccctggctc cgagccttta tcaatcgcat
     1621 aaacaagtcg tcgaaaagtt cccccaacaa ttcttccgtt tccgcgacat attgatcaat
     1681 atcctcgtcg gccaactttt ccgcggattc aaaaagaatc ctcaatcgat tcacttccga
     1741 cgtccggact tcggaacgag cgccttcatc cgcccccggg atcgccggtt ccggagcgcg
     1801 tgtttccggg cgatcattcg cctccacgct cccttttgtt tcagaattgg acatccgcat
     1861 ttccgggcga gtcgcgcccc cttgttgttt caattcttca atcacgctct gacgagtacc
     1921 ttcccatttt tcgcgcaagc ggtcatcccg caacagctcc ttctccaggc tgtccaaaag
     1981 cgacatgatt cctctttcag ccggagacga cctctgatgt tgaaaaccta gggagccaag
     2041 atgttgaagt acccattgca tctgtctaac gctcatcggc tcatagcttt cggcccggaa
     2101 accgggcggc acatgaacag cgggaacatc aaacatcgca acatgctgta cgcctacgac
     2161 tgcgagaagt cttttctttc cgggatactt tgccagttcc attgccagcc tgtctttgcc
     2221 aatttcgttc cgaagcaacg cgaccgtccg actgacctga ttcccggtac gaacaaacgc
     2281 attcatcgct tcttcagacc ctgctatttt ttgggcatac tgaaggagta tccggcgaac
     2341 cgattcttcc ggtaacccca tcgtcaatgt ccggctcttg attacattgg aaatggccgc
     2401 ctccgcggga cccccccggc ttacaatccg actcaattga aaaaacaaat cctgaaacaa
     2461 aataaccgcg gaaatttcct ccagagaaat ccccagtgac tcggcggtac gtcgcatcac
     2521 ctccgaatcc ccctgagttg gcagaggatc aatgatcggt atggtccccc ccgtgaggtc
     2581 ccgcaactta tagaccatca acacgtcaat cagtggaatt tcgggcacgg ccgacataat
     2641 tttatgtccc tcaaaaaaaa tgacccattc ttccggattt ttaaaaagag accgtgtcag
     2701 cgaaggatcc agtaaaagct ctgccgcccg gggagcgccg tgagccgtac caatgaggac
     2761 cgtttcccaa cctttaaaat ttacgtcaaa ccgtttcgcg ccgatcaccc gcatcggctc
     2821 gacgacatag gcccgcttca gactctcctg ataataatcc cgtaaactgt tttgaaattt
     2881 ctggacatca cgtcgagtca caaaccatat cccgtagtaa cccgcacccc ccaggacgcc
     2941 caagaggccc gccgccacac ccgcccggac aaaagcacga aatcctccgg atcgcttttt
     3001 cgcgacgtcc gcgatcgatt ccagaaaaaa aggcgttaaa gcttttttaa aatcggggat
     3061 cgcgtttaaa attttcttcc gggaaaaaaa tgtaatgcgt ccggcctcat cgtcccactg
     3121 caaaggttcg tccggtattt cgttttgctg ggcgcgtatc tccaatccgg taatggactt
     3181 cgctccaagt ttgtatccca cggcatccca gaccttcttg ctcatcctgg gatcggtcac
     3241 ccgacgatcc ttttcaaaaa ggtccacggg aacatttctt gcttccgaac gaacttgaga
     3301 agccgggaac agagcgagac gtccgtcctt taatccaggg atgccgcctt ctcccgcgct
     3361 tacgggcctg gagccgtaga aaaaagcttc gtccccgtca cgcgccaaag gaacgtagcg
     3421 caattccata cgggcaagcg aacggcgagc tccatcacgg acttctttag gttttccgta
     3481 tagaacggtt gcggaggcaa gatgaaaccc cgcggcacaa ttcccaaata gttctttgat
     3541 gcccgcaggt gtcactgggg tcaccggtct caggtaggaa cgcagggtca aataatcaac
     3601 ggtccccttc agggcgtcga gatatgtttt ggaatcccgg atttcggaca tcttcggagc
     3661 tagcgtcacg aaagagatcc ctttttcctt cagacctttc ttgatccctt ccgcgtgaaa
     3721 gcctccggtc accagaaccg cgcgggaaat cttccgttgc cgaatcgtct ccgtcatatt
     3781 ttcaagcatc gcttcatcac gtttcgcgac atcttcataa aaccggagcg cttcttcgta
     3841 cacagcgtcc aggtccggaa gcttcggatc ccgcgagcgg ttctccaatt gatccaaccg
     3901 actggcgatc acgcccggag cgatggagga tgccgccgtc tgaatttccc tgaaatcggc
     3961 ccggacaagc tgaaggccca gaagtttttc ggagagtcgg gcctcccggg acaggcggat
     4021 cagatcccgc tccagatcag tcgccgcaag ttttttcaaa atgcgttcgt ggatccgttc
     4081 cacttccgtg atcaaatcag gggcgtggat ctcgtcggtc aggatccgct cccccatttt
     4141 catcttgaga ttcggataag tcctgaaatc aaatccccgc gaggaggctt cggcataaaa
     4201 acgctcaaaa aattcccgca ccgaccggaa ttccagcggt agcttctttc cctccagaca
     4261 ggatttagtc tgggccgcga gatccctgag ccctttcgac tccagccatg ttttgagagc
     4321 gctccattcc accggtaggg catccgcgct tttttgttgt ttcagactcc cgctcaaacc
     4381 gaaaagtttt gtaaaacgga ccgcctgggg ccaatccatc tgctgataag gatcttcgag
     4441 gtcaagcgca agatgccgaa cggcataatc cttcagaatc tcccagcgat tcagccattc
     4501 ctggggtcgt tcctcgcaaa acacccacgt ctgaaagtat ctcttaagct ccggattgaa
     4561 tttccgggag gcaaggcttt tgatccggac gcggatccgc ccgaaaaact tttccgtcgg
     4621 gccgctgtgg ttcgcgacgt ggcggaacgt ttcaagattt tttaaataaa gggtcgcgtc
     4681 ctcgacgccg taggctgaaa atgtccgggc ggtttttcca ttttcccgtt ccgcaagaaa
     4741 catttccggg ccgccggcaa aaccttcgtg ggcaaggata tcgaccatct cccggttcag
     4801 gcccgggtcc ttgaaaacct tcagattggc gtcctccaac ctaccgcttc ccccttccag
     4861 aaaaagcgtc tgaacctgat aggtattctc cagatgctca agaatcgccc ggatattctt
     4921 ctgggcctcg tactgtccgt gcgggtcctg gatgtggatc acggtgacag gcgatgattc
     4981 aggccggtca gacgcgggga catttcgggg aacatacctt gtggtgactt gaccgaggga
     5041 ggacggaatc tcgataagat ccgccaaact ttgagcggga aattccgccc ggggatcagc
     5101 cgaaagtcga atggccggag ccgcccatcc cgtcgtggtg acgagaaaaa agaccacggt
     5161 cgccagggcc gttacccgta cattgggaaa aaatcctgaa atttttggtg tcatttgact
     5221 gggaaccagg gacggtgaga gatgaagtgc cccatgaaga actccccgta gtccgccgcc
     5281 gatccgtttc gctcctatct attgggtttg aaacacttta aggaaaggta cacggcgttt
     5341 ttccacttgt caaactgggt agcccctccc ccacggtaag gtttcaacat gaggcaacgg
     5401 gaggcaagat gatttcttac atcgtaatgc tataaatcgc tatatttaac accctaaaaa
     5461 aagtggccct ttaaaaagtc tcgaaataca acatccggga cattacacac aaaacaaaac
     5521 tacgcattaa tctcactttg aaaataaaat tgataccaca aatgagaatc ccgattggaa
     5581 gtgatatggt gcaaataaaa aatccctaaa aaagacagga taataagtcc cgcaagaatt
     5641 gcgttattca cagttttgtt tttgtacaag taataaacaa aataagaaag catgatcgac
     5701 tggaaaatat atagatatct gaaggggcgg ctgaaagaaa tagctcccac gggaaacatg
     5761 taatacgtga tgagccccac aaaatataaa aaataaatca tgttaaacca tctgcttgga
     5821 taatattttt ttagcctgcc actgtatatg atgacaatca cattaatagc aactttccac
     5881 aagtaagcta atcccgtccc ggtgctttct ttgaaatttt ccaccaacgc atcaatatta
     5941 tagcttgcct catacatttc agcgcctaat aaggaaatat aaaaatccac caccgcgcta
     6001 aagttcataa tgacatcaaa aaaaacctcc ttcaccacaa acgcggaagc aaaaaggact
     6061 aattgcaaag gaatgatctt taaataatct cttcctccga ccagtattgg gtaaaacaca
     6121 aacaaagcta tggcgcttcg gtgaaagaga aatgccatca caccaaacat tacatatctc
     6181 caaaactttt tctttacaat gaagttcagg gaacaaaccc agatacacgt ggcaattgac
     6241 tgcctgatta cattcatcca aaaaataata tctccattcg taaacaagaa aaaaatcaaa
     6301 agaggaaaca gataaatctc cttcttgaac gcgtaaaaga aaaaagcgac ctgaatgaac
     6361 gcaaggattg caaaataaat cacaaaattc aagtttaggg agtgagaaaa atcggttatt
     6421 acccgaaata acagctcatt tttcgacgca tctctattcg agagatacgc atccagatac
     6481 gcaaggtggt caactcccac atcatatctc attccgaaaa caaccgcgaa caacagcatg
     6541 gggaatataa ctgaaaagct ccaaaaggat ggcgttgagg tagtggaata ataatcaagc
     6601 accctataat caagcgcttt cgtctggcaa ccggcacgat aaaagcagag catcataatc
     6661 cctagtaaaa gggtataaat cgtcaagctt tgaatcattt tatccttgga tttaatcttc
     6721 tttcgttaag tgcttggcgt gaaattttca atgttgttcg gaaccaattc tggcaaccca
     6781 cgaacaaatt gccggttatt aatgctttgt agaataattt taaaaatggc cgaatcccga
     6841 atctcaaact ttgaattatc aaatacatca ccgacgtgtt tctattcaat ccatgctttc
     6901 ttaaaagatt gttttttctt atcctgatat caaacatact ttcgatattt ttaaaactcc
     6961 gggtattggt aatactcccc tgagtaactg tgacgcaata cacttcagcg gcatcggcat
     7021 atatcgattt ggcaactatc ccgagtttcg cgctaaaaac tgagtcctca gacacgggga
     7081 cttcctccca ccacatatca ttcgttttga ccaaaatagc tttaaccatt cttgaccagg
     7141 ccggcgacca tcgataccgc aaagcgtatt catccccgtc cttcaagaat ttgtctattt
     7201 tctcaatata taattcgtgt cgatgcgccg gctttagcgt gtcagaataa caactggtca
     7261 ttttaaaaaa aatcacgtca aacttcgcgt cccgatatga atcaaaaact tgaaaagcgc
     7321 gttccgtgaa aaaatcatcg gcatccaaaa acagcatcca gactcctttc gcctgttcca
     7381 tgccaacgtt tctcgcatgg ccacaacctt tcgtgttatc ggaaaacaag attcgcacgt
     7441 tgcttcgatg attaaacaga tgacgatcaa ttttattggg cgaattatca acgaccagca
     7501 cttcgatatc ttctctatcg gggactgatg ccacggccct ttccagaagc aatgtatcag
     7561 attttgtaaa atgaggaata ataacagaaa aataaatcat agctcctccg tacagtgtgc
     7621 catccgttta tctttttggg gaaattttgc cggattcacg cgcataatcc aatttgtttt
     7681 ttataccagc gggtgatgtc gttttcgcgg gcgggagaaa gttgtttgga gttaccggag
     7741 gcaacggtct tgccagtttt tgggggcgtc tggtcaaaca tgcgggaagg gagctcggtc
     7801 ctcactccca acgccttgca atggttaaga atttgcccgt cccctgtgac cacccgaacg
     7861 ttcgcgggga ctttttcgcg cctgatcatc gcgataatct catcgtccgc ctcaatgccg
     7921 ggggcggaaa acacgcaggt cacgccattc acctttcgcg acgatcgaac cggaaccccg
     7981 tccagcgagc ctttgtttcg atcaaacaca atcaccacct cggcgccacg ggactggcgc
     8041 cattctgaca tcagtctgac aagcccttcc ctcgcctcct gaaggcttcg ggacagaaga
     8101 cccgccagcc tcggcacctt gtggatcaca ttataaccgt caagaatata tttcacagat
     8161 tcggcccctt cctaaaaaat catcccggaa aaccggtcat tcatcagctt ccttcaccag
     8221 acgttcgact acggaccgcc agtccttttt caagaagcag attattcaaa aagatctcgc
     8281 ctccctcgcg atcggcgaag tagacatccg ccaaataccg gtcatatttg tccgaccggc
     8341 ttgaggtcag gagaatttga tccgcgccgc gaagcctcga cttcacaaac gtggaggcct
     8401 gttttccctc ttccgtcgcg agttcaggac agtccacgcc acgaagcctc aagtactgcc
     8461 gcgtacgggt gttaaaaccc aggtccacaa tcacccgcag agtgtcaccg tccacgacct
     8521 tttcaacagt cgcctggtaa gtgtaaagcg aggcgggttc cgcgcccgga agcttgtcca
     8581 gctgatactg atcctccccc gtcctgacgg attgaacaat ctccccagac gtgaaggtcc
     8641 ggccagtgac cgcgtcaagg tcacggtaac aggcaaaccc gagatccaga aaccgtaaat
     8701 tcgcttcgcc ggactgaaag gttttgggat ccaggatccg gtacgcgtaa agaatcccgc
     8761 gtcttgggac gagaagttcc gcgggtcgcg gagctgaaac gtccgcgttt cccgatgttt
     8821 tcgaggccag gtttccggcc acctgttcac gaaccatttc ctgccggaca aggcgacgga
     8881 gctgatcacg cttcagccgt tcccggatca cccgtcgcac gagcgccttg cgctgtttga
     8941 ggttgcccac ccgcaggagt tcgacgtgtt gtgaccaacc caattccacg gcgggccgtg
     9001 aatttgagta ggccagataa aatcgccgca tgtccagaag ctggttgggt gaaaatccgg
     9061 ggcccagccg ccgcgtaagg tcttcggaaa gttgcgtgat gagaccgcta ccgtacgccg
     9121 atcgaatctc ccctttctgc tcgacttgga caatacattg cccgattcgc cagtaggctt
     9181 ccacgatcgc cttccgcgcg cttccgcaaa gggcggagat gtcggtaagg agttttgtgt
     9241 agcttgcggg agcgggaagg tttgccatgg ccgaaattat accaaaagaa ggcgtaacag
     9301 gaaaaagaga ttacacaatc cggcgccaaa gacctttcgc ggggtgaagt gcgaagaaac
     9361 agccacacat ctttttaaat tgcggcgttg tagcaaaaaa aacagcgcaa cccggcgatt
     9421 aaattccctg cttttcctta ctggtgatcc cccgacagga ggtcagaatc taccgaaagg
     9481 accgcgtacc gttcttctgg tgcttgtaac
//
LOCUS       DAOSIO010000047         9324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_7059_length_9324_cov_4.109397, whole genome shotgun sequence.
ACCESSION   DAOSIO010000047 DAOSIO010000000
VERSION     DAOSIO010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 9324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9324
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7059_length_9324_cov_4.109397"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..1109
                     /locus_tag="PK590_02965"
     CDS             249..1109
                     /locus_tag="PK590_02965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOG23591.1"
                     /translation="MKRKALSLMCLSVLFTYAAMPAAYAARDMIQIKGSDTMVNLGQA
                     WAEEFMMQNPEASIAVTGGGSGTGMAALISGTCDIAESSREIKDQEMEAARKNGHDVH
                     EFIVAVDAIAVVVHPSNPVKQLTIEQLSGIYTGKIKNWNELGGKDQKILALSRERNSG
                     THVYFLEEVVRMGVEKGPEQFAPEVLMLPSSQAIISEVSQSETAIGYVGLGYVSDRVH
                     IPAIALSNQGPFVSPTLETTMDKSYPLSRSLLFYTGREPEGTVKNFIDFVLSEEGQEI
                     VRIMDFVPLR"
     gene            1114..1980
                     /gene="pstC"
                     /locus_tag="PK590_02970"
     CDS             1114..1980
                     /gene="pstC"
                     /locus_tag="PK590_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOG23592.1"
                     /translation="MKKFFEKIIEKIILLNGFACILFVILIFFFLAKDGLALFKVSSP
                     IQFFFGLLWNPLAEPPQYGIVPLLLGSIMVTLGAIAISVPLGLACAIFIGEIAPRWMR
                     EILKAGVELLAAIPSIVIGFIGMTMLAPFVKNLFGLPTGLTALTGSIALAFMAIPTIV
                     SIMEDAITAVPRTYKEGSLALGATHWQTIYRVTIPAASSGIVASIMLGIGRVIGETMA
                     VMMITGNASVIPHSLLQPVRTMTATIAAEMGETVIGSDHYFALFAVGLVLFAITFVIN
                     FFADMALHQQKK"
     gene            1983..2828
                     /gene="pstA"
                     /locus_tag="PK590_02975"
     CDS             1983..2828
                     /gene="pstA"
                     /locus_tag="PK590_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOG23593.1"
                     /translation="MKPQTKQKIAFTCLGLAAVVVIIPVIVIFVILIDKGISAINWQF
                     LSTMPRMGMRAGGIYPAIMGTVYLVSGTILVALPLGVLAAIYLVEYAKRNWWTRMIEI
                     AIVNLAGVPSVVYGLFGMGFFVIFFKFGASILAGSLTLAIMILPVIITASKEALMSVP
                     HSFREASLALGVSKWQTIRCVVLPNALPGILTGTILGISRAAGETAPILFTVAAFYLP
                     RLPKSIFDQCMALPYHLYVLSTQVPNVKESLQYGTALVLVGMIFLLNLVAIIIRSHMR
                     SKKQW"
     gene            2952..3722
                     /locus_tag="PK590_02980"
     CDS             2952..3722
                     /locus_tag="PK590_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HOG23594.1"
                     /translation="MNPIPDRANKITVEDVSILFGEVRALNHVSFSVKSNEILSVIGP
                     ANSGKTSFLRAMNRMNDLDIACRMTGSIRLNGQDIQKEMDAEDVRRKVTMVFALPLPL
                     PMSIFDNVAYGPRRHGIKNKRKLAEIVEKSLKAAFLWDEVKDRLSSSGMKLSGGQQQR
                     LCIARTLAVEPEVILYDEPCSGLDPISTAKVEDAMFELKKRYTQILVTNNVKQAARVG
                     DRTAFFLTGELVEIGETGKIFTSPRDQRTQDYITGRFG"
     gene            3810..4562
                     /gene="pstB"
                     /locus_tag="PK590_02985"
     CDS             3810..4562
                     /gene="pstB"
                     /locus_tag="PK590_02985"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOG23595.1"
                     /translation="MSKIETKNLDLFYGSFHALLDINLEVEEKMITALIGPSGCGKST
                     LLRALNRMNDLIEGVRITGEVCVDGKNIYEHGTDLIGLRKKVGMVFQRPNPFPLSIFD
                     NIAYGPRIHGIKNRGELEGIVQKALSDVMLWDVLKDKLDRFALNLSIEQQQRLCIARL
                     SAITPEIVLMDEPCSALDPMATQRIEELMFELKKNYTIVIVTHNMQQAARVSDKTGFM
                     LLGKLVEYGDTKQVFTTPHEKQTEEYITGRYG"
     gene            4695..5357
                     /gene="phoU"
                     /locus_tag="PK590_02990"
     CDS             4695..5357
                     /gene="phoU"
                     /locus_tag="PK590_02990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOG23596.1"
                     /translation="MERRFSEELKTLKQKLFQMGLLVEGAIQKAIDAMQTRSADLVEG
                     VFQDEEMINAIEIEIDDKGHALFALSQPVAGDLRLLAMILKINTDLERMGDHAVNIAQ
                     CSKRLLADAPLAIDFPLPKMGESVQRMVRDALDCFMNENVDLARRVLESDDKIDDFND
                     QLYESMVAMMEKDSSTVRRALRYVVIGRNLERIADLSNNIAEDTIYLKQGKEVRHHFE
                     KQ"
     gene            complement(5455..7293)
                     /locus_tag="PK590_02995"
     CDS             complement(5455..7293)
                     /locus_tag="PK590_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG23597.1"
                     /translation="MKPPESSLSKGSAIVLTEIKMPLPRIGFVRRFMALYGLVFIFVF
                     AAAGFFISHHFKQSMMAQLQESLVHQSLMIREIIRGELPMQPALLQSKISALAEGLEI
                     RITYIDSNGLVLADSKEKLANSIQTMDNHASRPEVAAAMRGDTGISTRYSMSLGKQML
                     YVAVPILEGDNLLGVVRVAMPSSRIQNVLNHAKRPVSVIVLIAVIIILLSGSLFANHW
                     AKRLRTITRGAVRYARQNWSEKILVEGRDELRILGDAMNRMAGALSSRIADLESEKGK
                     ISTILSHMSDGVIAINRKKEVVMANPMAEKLFGFSTDTIRGKSLIETTRHPYLEIIVD
                     QAFTQETVVTGEIQLIGNEKKILRASVVATTKEIRDISGILVFHDITELRRLEQIRKE
                     FVANASHELRTPLTSLKGFIETLRDGAEKDPKTRKHFLTMMHEDATRLGHLVEDILTL
                     GEIEQRILPLKKETLDLASEIRESLARFEPQLKAKKLTVNDHLTTTPFFIQGDRDKIR
                     QVFVNLLDNAVKYTPEGGSIFLSAEKKSGKVKVTIRDTGPGIPEDTIPRIFERFFRVD
                     KARSRELGGTGLGLAIAKHIMESHDGSIDCQSVLGKGSSFSVSFPL"
     gene            complement(7235..7924)
                     /locus_tag="PK590_03000"
     CDS             complement(7235..7924)
                     /locus_tag="PK590_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG23598.1"
                     /translation="MATETILLIEDERNIAELVKYNLDREGFHVICVSSGRAGLEAAQ
                     KNPPTLILLDLMLTELGGLEICKILKENPKTRAIPVIMLTAKSTEADKVLGLELGADD
                     YITKPFSPRELLARIKAVLRRINTSPEREILEAGSIELDLGKHELRVKEKPVEITAKE
                     FALLEAMMASKGRVLTRETLLGKVWGYENSTRLETRTVDMHITQLRKKLKNEAPRIVT
                     LKGVGYRFDGD"
     gene            8262..>9324
                     /locus_tag="PK590_03005"
     CDS             8262..>9324
                     /locus_tag="PK590_03005"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOG23599.1"
                     /translation="MAPAEHEESEAFFLKRIYRDFFETVQRQKYWIALFSFCVVLLVA
                     IFSFLQTPYYQATTTLLIDSDMAQDPSEQKSLGNLELQVAIIQSPEIIAKVIEQTNFS
                     VDPSVDSRVDPREVFMRFLDVKSKKQNKTIDISFLSENAETAALTANQIAQAYIEGKT
                     TKSSGVVVDSVESLQAQLAQQVEKHNAVKNKIAELNRENPELGNENILADQIKYLNSE
                     YVKADSRTMELRNTLSELELIIKSGGNIQDHPYAASHPRVRQKMDRIRDTEIQLLEME
                     QEYRQMHPTVLKAQAKLKALRQSLDDEKTQLIAELNAALKASEAMTQGIRQSMLELQA
                     KEKDFSPAQLAYKNLLTEET"
BASE COUNT         2259 a         2132 c         2515 g         2418 t
ORIGIN      
        1 tcgcctccgt cggattttcg tagattcatt ccgcagtctt gggtaaacta tggcactaaa
       61 cggggggcgg ggggcccgac ccacttaccc tgaaggacgc cctgcttctc ggggcggcat
      121 tgaagagttt ccttaatgag agatccttcg ccttcgctcc caacgggtct gaagaccctc
      181 tgggccgccg acaggataat tcccccggaa ataatatccc gagggaacaa ctcaattatg
      241 ggggagttat gaaacgtaaa gcgctgagtt tgatgtgttt atccgttctt tttacttatg
      301 ccgcaatgcc ggccgcttat gccgcccgcg atatgatcca gatcaaggga tccgatacca
      361 tggtcaatct tggtcaggcc tgggccgagg aatttatgat gcaaaatccc gaagccagta
      421 ttgccgtgac ggggggaggc tccgggaccg gaatggcggc tctaatcagc gggacctgcg
      481 acatcgcgga atcttcccgg gaaataaaag accaggagat ggaggccgcg cgcaagaatg
      541 gtcatgatgt ccatgaattt atcgtggcgg tggacgcgat cgcggtggtg gttcatcctt
      601 ccaatcccgt caagcaactg acgatcgaac aactctcggg gatttatacc ggaaaaatca
      661 aaaattggaa tgaactcggg gggaaagatc agaaaatcct tgccctttcg agagaacgga
      721 actcgggaac ccacgtttat tttctcgaag aggttgtccg gatgggagtt gaaaaagggc
      781 ccgaacagtt tgctcccgag gtattgatgc ttccttcgtc ccaagccatt atttccgagg
      841 tgtcccagag tgaaactgcc attggctatg tcggacttgg ctatgtttcc gatcgtgtcc
      901 acattccggc gattgccctg agcaaccaag ggccttttgt cagtccgacg ctcgaaacga
      961 cgatggataa aagttatccg ttgtcccgtt ctctgctgtt ttatactgga cgtgaacctg
     1021 agggaaccgt gaaaaatttt atcgattttg tcctgagcga ggaaggccag gaaatcgtca
     1081 ggatcatgga ctttgtcccg ctccgataat accgtgaaga aattcttcga aaaaatcata
     1141 gaaaaaatca ttctgcttaa cggctttgcc tgcatcctgt ttgtgatcct gatttttttc
     1201 tttctcgcca aggatggtct tgcccttttt aaagtcagtt ctccgattca gtttttcttt
     1261 gggttgttgt ggaacccgct cgcggaacct ccccaatacg gtattgtgcc gctactcctt
     1321 ggatctatca tggtgactct tggagcgatc gcgatctcgg taccattggg cctggcatgc
     1381 gccattttca tcggggaaat agccccccga tggatgcgcg agattctcaa ggcaggggtc
     1441 gagcttctcg cggccattcc gagtattgtg atcgggttta tcgggatgac aatgctggcc
     1501 cctttcgtaa aaaatctttt tggcctgccg accggtttga cggcattgac gggatcgatt
     1561 gccctggctt ttatggcgat cccgacaatc gttagcatta tggaggacgc cattacggcc
     1621 gttccgcgaa cgtacaagga aggttctctg gcccttgggg cgacccactg gcagacgatt
     1681 tacagggtga ctattccggc ggcgtcctca gggattgtcg cgtcgattat gctggggatc
     1741 gggcgtgtga tcggggagac gatggcggtc atgatgatta ccggaaatgc ctccgtcatt
     1801 cctcattctc ttctccagcc ggtgaggacg atgacggcca cgatcgcggc tgaaatgggg
     1861 gaaaccgtga taggaagcga ccactacttc gcccttttcg cggtaggact tgtccttttt
     1921 gccattactt ttgtgattaa ctttttcgcc gacatggcgc tgcatcaaca gaaaaaataa
     1981 taatgaaacc acaaaccaaa caaaagatcg cgtttacctg tcttggcctc gcggctgtcg
     2041 tcgtgattat tccggtaatc gttatttttg tgatcctgat cgacaagggg atatccgcga
     2101 tcaactggca gtttctcagt accatgccac gaatggggat gcgggccgga gggatttatc
     2161 ccgcgatcat gggaaccgtc tacctcgtgt cgggaacgat tcttgtcgcg ttgccgctcg
     2221 gcgttttggc cgctatttat cttgtggaat atgccaagcg gaactggtgg acgcggatga
     2281 tcgaaatcgc gatcgtcaat cttgcagggg ttccttccgt cgtttacgga ctcttcggga
     2341 tgggattttt tgtaatcttt ttcaagtttg gggcctctat tctcgcgggg tccctgactc
     2401 tggcgatcat gattttgccg gtgattatca cggcgtccaa ggaagcgttg atgagcgttc
     2461 ctcattcttt tcgtgaggcg agccttgccc ttggagtcag caagtggcag acgatccgtt
     2521 gtgttgttct tccgaacgcc ctgccgggta ttttgactgg aacgattctt ggaatcagtc
     2581 gcgcggccgg tgaaaccgct cctatccttt ttacggtggc ggccttttat cttccgaggc
     2641 tccccaaatc aatttttgat cagtgtatgg cgcttcctta ccatctctac gttttgtcga
     2701 cccaggtgcc taatgtcaag gaatcccttc agtacgggac ggcgcttgtg cttgtcggga
     2761 tgatttttct cctgaatctg gttgcgatta ttatccggtc tcatatgagg agtaagaagc
     2821 aatggtaagt accgttaacc ttgttagcga ccggtatgcg gcgtggcggt attttttgcg
     2881 aaaacaaatg gagtgagtcg atgacacttt tggcgaataa ggaatgtcct gtttctgaga
     2941 aaactgggga aatgaatccg atccctgaca gggcgaacaa aatcacggtc gaggatgtca
     3001 gtattttgtt tggcgaggtc agggcattga atcacgtgag tttttcggtc aagtcgaatg
     3061 aaattttaag cgtgatcggc cctgccaaca gcgggaaaac atcttttttg cgggcgatga
     3121 acaggatgaa tgatttggac attgcctgcc gcatgaccgg ttcgatccgg ttgaacggcc
     3181 aggatatcca gaaggagatg gatgccgaag acgtgcggag aaaggtcacc atggtttttg
     3241 cgttaccgct tccgctaccg atgtcgattt ttgacaatgt ggcttatgga ccgagacgcc
     3301 acgggatcaa aaacaagagg aagctcgcgg agattgtcga aaaaagtctg aaagctgcct
     3361 ttttatggga tgaagtcaag gatcggttgt cgtcatcggg aatgaagttg tcgggcggac
     3421 aacagcaacg gctttgcatc gcacggacac ttgccgtgga accggaggtc attctttacg
     3481 atgagccatg ttcggggctt gacccgattt cgaccgccaa agtggaagac gcgatgtttg
     3541 agctaaaaaa acgctacaca cagattcttg tgacgaataa cgtgaagcag gccgcgcgag
     3601 tgggggacag gacggctttt tttcttacgg gtgaactggt cgagatcggt gaaacgggaa
     3661 aaattttcac ttccccccgc gatcagagga cgcaggatta tattacggga aggtttggtt
     3721 aacgcgggaa ggccgatgat ggaagatgga tggagaaaaa cggattttac tccatgcgta
     3781 cgagcgccct ccgaaagttg aagaccacga tgagcaaaat cgaaaccaaa aatctggatc
     3841 ttttttacgg tagttttcac gctctcctcg acattaatct tgaggtcgaa gagaagatga
     3901 tcacggcact gatcgggccg tccggttgtg gaaaatcgac gcttctcagg gccttgaacc
     3961 gaatgaacga cctgattgaa ggggttcgga tcacggggga ggtttgcgtt gacggaaaaa
     4021 atatctatga gcatggaacg gatttgattg ggcttcggaa aaaggttgga atggtgtttc
     4081 agcgcccgaa tccgtttcct ctttccattt ttgacaacat cgcgtacggg ccgcgcattc
     4141 acgggatcaa aaaccggggt gagctggagg gaattgtcca gaaagcgcta agtgacgtta
     4201 tgttgtggga tgttttaaaa gataaactcg atcggttcgc gttaaatctt tccattgaac
     4261 agcagcagcg tctctgtatc gcgcgccttt ccgcgattac gcccgagatt gttttgatgg
     4321 atgagccttg ttccgcgctt gaccccatgg caacgcaaag gatcgaggaa ctgatgtttg
     4381 aacttaagaa aaattatacg attgtgatcg tgacgcacaa tatgcagcaa gccgctcgcg
     4441 tttctgacaa aacgggtttt atgttactcg ggaagcttgt ggaatacgga gataccaaac
     4501 aggttttcac gacgccgcac gaaaaacaga ctgaagagta cattacggga aggtacggat
     4561 aatgaggatg ctgtgcgtta gatgcgtagt ttcgcaaaac aaagcaccgg agttaattca
     4621 aagacggcca aagagcagct tgcaatttat ggttgccacg ctccgtaaat tgtttgctca
     4681 ttaaggaggc gattatggaa cgacgttttt cagaagaact caaaacacta aaacaaaaac
     4741 tctttcaaat ggggcttttg gtggaagggg cgattcagaa ggccattgat gcgatgcaga
     4801 caagatccgc ggatttggtg gagggggttt tccaggatga agaaatgatt aacgcgattg
     4861 agatcgagat tgacgacaag gggcacgccc ttttcgcgct gtctcagccg gtggcggggg
     4921 accttcgcct tcttgccatg attctcaaaa tcaacacaga tcttgaacgg atgggggacc
     4981 acgcggtcaa tattgcccag tgcagcaaac gcctgctggc tgatgcgccg cttgcgatag
     5041 attttccttt gcccaaaatg ggagaatcgg ttcagcgaat ggtgcgtgac gcgctggatt
     5101 gctttatgaa cgaaaatgtt gatctggccc gccgggttct tgaaagcgac gacaagattg
     5161 atgatttcaa tgaccagttg tatgagtcga tggtggcgat gatggaaaag gatagctcga
     5221 ccgtgcgccg cgcgcttcgt tacgttgtga tcggaagaaa tctggagcgg atcgcggatc
     5281 tctccaataa catcgccgaa gacacgatct atctcaaaca aggcaaggaa gtacgccatc
     5341 actttgaaaa acaataagaa tcggaacttt cggcaaggag acttccagcc cgatctttag
     5401 cgaggtcagg agacgggaag tttggcgggg agtcgcagag agacagtttg ccgcctagag
     5461 cgggaatgaa acagaaaaac tggaaccttt tcccagaaca ctttggcagt cgatggagcc
     5521 atcgtgcgat tccatgatgt gtttggctat tgccaggcca agtcccgtcc cgcccaattc
     5581 gcgtgaacgc gccttatcga ccctgaaaaa ccgttcaaaa attctgggga tggtatcttc
     5641 cggaatcccg gggcccgtat cccggatggt gaccttgacc ttgccgcttt ttttctccgc
     5701 ggaaagaaaa atactgccgc cttccggcgt gtatttgacc gcgttatcca gaagattgac
     5761 aaagacctga cggattttgt cccggtcgcc ctgaatgaaa aacggagtgg tagtcaggtg
     5821 gtcattaacc gtcaattttt ttgcctttag ctggggttca aaccgcgcca gcgattccct
     5881 gatctcggac gcaagatcca gagtctcttt ctttagggga aggattctct gttcaatttc
     5941 cccgagagtc aggatgtctt caactagatg acccagtctc gtggcgtcct catgcatcat
     6001 cgtgaggaaa tgttttcggg ttttgggatc tttttccgct ccgtcgcgaa gagtttcaat
     6061 aaagcctttt aatgacgtca ggggagtccg gagttcgtga gaggcgttgg ccacaaactc
     6121 tttcctgatt tgctccagac gacggagttc ggtgatatcg tgaaaaacaa gaatgccgct
     6181 gatgtcccgg atttctttcg tggtggccac gacgctcgca cggagaatct ttttctcgtt
     6241 gcctatgagc tggatttctc cggtgacgac ggtctcttgc gtaaatgctt ggtcgacaat
     6301 gatttccaga taggggtggc gggttgtttc aatcaggctt tttcccctga tcgtgtccgt
     6361 tgaaaacccg aaaagttttt cggccatcgg gttcgccatg acgacttctt ttttccggtt
     6421 aatcgctata acaccatcac tcatgtggga caggatggtg gaaatttttc ctttttcact
     6481 ttcaagatcc gcgatccggg aggacaaagc cccagccatc cggttcatgg cgtcaccgag
     6541 aattctcagc tcatcacgcc cttccaccag gatcttctct gaccagtttt gccgggcgta
     6601 gcggaccgct ccccgggtaa tggtccgaag gcgtttcgcc caatggttgg caaagagaga
     6661 gccggatagc aggattatga tcacggcgat gagtacgatg acggacacgg ggcgtttcgc
     6721 atgattaagg acattctgga tccgcgaaga gggcatggcg acacggacga cgccaagaag
     6781 gttatctcct tccagaatgg ggacggcaac gtagagcatt tgcttaccca ggctcatgct
     6841 gtatcgcgtg ctaatcccgg tatcaccacg catggcggcc gcgacttcag ggcggctcgc
     6901 gtgattatcc atggtctgga tcgaattggc caatttttcc ttcgagtctg ccaggacgag
     6961 accgtttgaa tcgatataag tgatccggat ctccaatccc tccgccaggg ccgagatttt
     7021 actctggaga agcgctggtt gcatgggaag ttctccacga atgatttccc ggatcatcaa
     7081 agattgatgg acgagggatt cctggagctg ggccatcatg ctctgtttga aatggtggga
     7141 aataaaaaaa ccggcggcgg caaaaacaaa aatgaaaaca agtccgtaca gcgccatgaa
     7201 gcgtctgacg aatccaatcc ggggaagagg cattttaatc tccgtcaaaa cgatagccga
     7261 cccctttgag agtgacgatt cggggggctt catttttcag ttttttgcga agttgagtaa
     7321 tatgcatatc aacggtcctt gtttcaaggc gggtggaatt ttcataaccc caaactttac
     7381 ccagtaaagt ctcccgggtt aatacccggc ctttgctggc catcatggcc tccagtagcg
     7441 cgaattcttt cgcggtaatt tcgacaggtt tttctttgac cctgagctca tgtttgccga
     7501 gatccagttc gatgctccca gcttcaagga tttcccgttc cggtgaggtg tttatcctgc
     7561 ggagtacggc tttaattcgc gcaagcagtt cccgagggct aaaaggtttt gttatgtaat
     7621 catccgctcc cagctcaagg ccgagcactt tgtcagcctc ggtgctttta gcggtgagca
     7681 taattaccgg aatggcccgt gttttggggt tttctttgag gattttgcaa atctcaagac
     7741 cacctagttc cgtgagcatg aggtcgagaa gaatcagggt ggggggattt ttttgagcgg
     7801 cttcaagtcc cgcgcgaccg ctggacacac agataacatg aaacccctcc cggtcaaggt
     7861 tgtatttgac cagttctgca atgtttcttt cgtcttcgat gaggagaata gtttcggttg
     7921 ccataggcat ataaaatcct gtatagtcta gcacgatttt aaataaagtg gagtcatgaa
     7981 ggctgtctca tccgtttatt tttcaaggaa atgatgccgt gtgggagctt ctccgggacg
     8041 gaaccaccgg gaagggcgtt gatttggttt ctcagaagtg gttgtttaag ctttgatgtg
     8101 aggatgccgt tactcaatat cgggcttttt tgggtttttt gtggccttgt attttaatct
     8161 gttgagggcc tggatgttcc tgcggtcaaa acaaggaatt ttagaaactg aagcacagtc
     8221 agacaaaggt agagtcgaga atggataacg tgaatgatcc tgtggcacct gctgaacacg
     8281 aagaatcgga agctttcttc ctgaagagga tttatcgtga tttttttgag actgtgcaaa
     8341 gacagaagta ctggattgct cttttttctt tttgcgtcgt tttattagtt gctatttttt
     8401 ccttcctcca gaccccctac taccaggcca caactaccct actgattgac tctgatatgg
     8461 cccaggatcc ttcggagcaa aagagcctgg ggaatttaga attgcaggtt gctatcattc
     8521 aaagtccgga aattatcgcg aaagtcatag aacaaaccaa tttttcagtt gatccgtccg
     8581 tcgattcccg tgtggacccg cgtgaagttt ttatgcggtt tttggacgtg aagtcaaaaa
     8641 agcaaaataa aacgatcgac atttcatttt tgagtgaaaa tgccgagacg gcggcgctta
     8701 cggcgaatca aatcgctcag gcctacattg aaggaaagac cacgaaaagc tctggtgtgg
     8761 tagttgactc tgtggagtct ttgcaagcac aactcgctca gcaggttgag aaacacaatg
     8821 ccgtcaaaaa taagattgcg gaattaaacc gtgaaaatcc cgaacttggt aacgagaata
     8881 ttttggcaga tcaaatcaaa tatcttaatt ccgaatacgt caaagccgat tcccgcacaa
     8941 tggaattgcg aaatacgctt tcggagttgg agttaatcat taaaagcggc gggaacattc
     9001 aggatcatcc ttacgctgct tcgcatcccc gagtgagaca aaaaatggac agaattcggg
     9061 ataccgagat acagcttttg gaaatggagc aagaatatcg gcagatgcat ccgacggttt
     9121 tgaaagcgca agcaaagctt aaggcgctcc ggcagtcgct ggatgatgaa aaaacgcaac
     9181 tgatcgcgga actgaatgct gccctgaaag ccagtgaggc catgacgcaa ggaattcggc
     9241 aaagcatgct tgagctacag gcgaaagaga aagatttttc acctgcccaa ctggcctaca
     9301 agaatctttt gacggaagaa acgt
//
LOCUS       DAOSIO010000048         9192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_7165_length_9192_cov_4.443690, whole genome shotgun sequence.
ACCESSION   DAOSIO010000048 DAOSIO010000000
VERSION     DAOSIO010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7165_length_9192_cov_4.443690"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="PK590_03010"
     CDS             <1..1235
                     /locus_tag="PK590_03010"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOG23600.1"
                     /translation="GGGQGKRLFPLTSYRSKPAVPIGGKYRLVDIPISNAINSGLKRI
                     YVLTQFNSASLHRHIHRTYPYESFTKTSIELLAAEQTVDNKDWFQGTADAVRKCIPHY
                     HMEGVDTFLILSGDHLYQMNYGEILGFHQMQKADVTVATIPVPERLIKGFGIMKLTKD
                     GRITEFLEKPSKDDDPSSFIMPKTLCGLHQVKSKKPMYLASMGIYVFRREALLEALKG
                     NEKDFGKEVIPKAIENYKVFGYVHTGYWRDVGTIGSFYEASIELTHQNPPFSFLIPDG
                     RVFTRPRFLPPSKIIDAQLDHALMSEGCILTGAKIEHSVVGLRSIVGHGTVIRRSVLM
                     GNDHYEWPKPGEDRTIGIGKNCLIENCILDKNVGIGDNVQITNSRGRKEEDGENYYIR
                     DGIVIIPKGGYIPAGTKI"
     gene            1238..1516
                     /locus_tag="PK590_03015"
     CDS             1238..1516
                     /locus_tag="PK590_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23601.1"
                     /translation="MKGLLFFLTAFFMVFSSGMLCRALPADTAYGDAIEDGGQGSVTS
                     AQSTLASLFGEKAVISIPPQIARDATIAIPSFDSRELEIEKNDLWERN"
     gene            complement(1678..2775)
                     /locus_tag="PK590_03020"
     CDS             complement(1678..2775)
                     /locus_tag="PK590_03020"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOG23602.1"
                     /translation="MKVLDRYLVRELLIPIIFCTVTLVFLILIADLFDNLDELLRNKT
                     PILVILRYYLCLIPMAFSQTIAWSVWLGTIFLLVNLGAHNELIAMKAAGLKIMTIVRP
                     LIFLAILIGIVTFLVTDRIVPTTYKTANELLEIHIEKKKASGDRKVLKNVTYYSGGNV
                     LYYFRAFTPQNREVADAIILWLDPKTHHTTRKVFAKEGTFAEGAWTFRGITEYQTDVQ
                     GAVLGSPVTIPEKIYPDIKVTPRDLIMATSESIYLSYHELQYTINKLKENGIAVYSEE
                     VDLYDRLAAPWKGLVMMLIAIPFMASIHTRKSIAINVLICAALVFAYHVFGAVSIAIG
                     KSGKFIPFLSAWGGNILFAAFAFIRFERANY"
     gene            complement(3011..3724)
                     /locus_tag="PK590_03025"
     CDS             complement(3011..3724)
                     /locus_tag="PK590_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOG23603.1"
                     /translation="MRHFKESLIRLLYPAHCEFCGKPLALDENLLCETCEQNLEGLAY
                     PLEKSLVDEQFENLDHAWAVYAYVTPLRELLQAVKYRRKDYLLKAFKNPAAKLATSIA
                     SEYVYNALIPIPLNRVKRIQRQFNQSQILADLLQPFFKLPIWSNLLKKKYFIPSQTSM
                     TREERVINIYGVFKVSSAKQTEGKSFLLVDDVLTTGATASEAARTLKKAGAKRVDLFA
                     LAKTNLPIKKEKSSSDEHP"
     gene            4004..5566
                     /gene="rpsA"
                     /locus_tag="PK590_03030"
     CDS             4004..5566
                     /gene="rpsA"
                     /locus_tag="PK590_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOG23604.1"
                     /translation="MQSQFAELYEKSFQSVVPGDVVTGTIIEVRQKEVVVDIGYKAEG
                     VLPFDEFTDPQSLAVGQEVEVLFEGFDDREGTALLSKRKADRQKTWNDILSNAEEGAI
                     VEGRISRKVRGGFMVDIGMEAFLPASLVDIRPVRNQDAFVGLQSKFLVVKINHKRKNV
                     VVSRKDLLEKSRQEARSEKLNELQVGQIVHGKVKNITDFGVFIDIGNLDGLLHITDMS
                     WGRISHPSDLVKIGDEIDVAVISIDKEKQKISLGLKQKSSDPWETVEDRYAIGNLVHG
                     RVVNILPYGAFVELEPGIEGLVHISELSWTKRVTHPSEVLSIGQQLDVVILSLDTTAK
                     KISLGARQAQENPWDKVADKYQVGSRVQGTIRNLTDYGAFVELEPGIEGLLHVSDLSW
                     THKVAKPSDLLQKGETMEVMVLNVDTEAKKISLGLKQMQDDPWNELTKDILTGLEIQG
                     KITRVVNFGVFVELDNGLEGLIHVSELPEKSGDDLVKLYKVGETIRCSVLHVDHDGRK
                     IALTCKNESISA"
     gene            5569..6825
                     /gene="fabF"
                     /locus_tag="PK590_03035"
     CDS             5569..6825
                     /gene="fabF"
                     /locus_tag="PK590_03035"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOG23605.1"
                     /translation="MLTSNGQNRRVVITGIGVISPIGSGKAAFWDSLSKGKSGISPIS
                     SFDTSAFTTKFGGEIKDFDPSAHILPKKLKRMDRTTQLAVVASKMAVEDAGLNFQEDG
                     YRERTGVIIGTALAGVGYILEQHDILRERGPMRLNPFTALASFPDACAGNVSIELGVT
                     GPSFSLATACSSASDACGYAFDAIRSGRLDFLIMGGAEATIFPGIMASFCVARALSTR
                     NDQPEKASRPFSADRDGFVLGEGAGMFVVEEYERAQKRGAHIYGEILGHGMTCDAYHM
                     TIPDPEGKEAGRAMSLALSEAGLRPDEIDYINAHGTSTPLNDKTETMIIKKVFGDHAR
                     KLPISSTKSMIGHLIGAAGSVELIATLLAMENQVIPPTINYGSRDPECDLDYVPNEAR
                     PAKIRIAMKNSFGFGGKNSILILKKV"
     gene            6912..7784
                     /gene="lpxA"
                     /locus_tag="PK590_03040"
     CDS             6912..7784
                     /gene="lpxA"
                     /locus_tag="PK590_03040"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOG23606.1"
                     /translation="MSNIIHPTAIIGSNVSLGDENSIGPCVTIENGVKLGRRNRIFQG
                     TFIGAGTELGDDNEIHMNAVIGHTPQDIAYKNEDTATKIGNKNVIREFVTIHRGTKAG
                     TSTVIGHENFIMAYCHIAHNCCLGNRIIMVNQASLTGHCVVEDRAFLSGMTGFHQFTR
                     IGMLAMVSALSAFNKDIPPFVICGGRPGVAQGINVVGMRRAGIGPEVRKEIKESYRLL
                     YRSGLNTTQAIEAIKKSFKSPEVAHFVKFIETSRRGIIDGSGAVADTIVPRKNQGAGA
                     GESADDDLIASPDV"
     gene            7863..>9192
                     /gene="pyk"
                     /locus_tag="PK590_03045"
     CDS             7863..>9192
                     /gene="pyk"
                     /locus_tag="PK590_03045"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOG23607.1"
                     /translation="MAVLPGHKTKIVATIGPASESREMLERLINAGMNIARLNFSHGD
                     FPAHGKVIRRIREAEKATGKKVAIMADLSGPKMRLGSISPEPVELKAGDTFILTSDDI
                     IGNSQRVSMSFKSLPKVVKSGDKLFLNDGLVQLVVEDVSGNDVRCKVLVGGELRSRKG
                     LNLPGIHLGIAAFTERDREGLEYALANGVDAISQSFVENAGDIEIVRQAAIRMGKNPF
                     IIAKIERAGALDQLDEILKAADGIMVARGDLGVEVPIEQIAITQKKVIAKANLAGKPV
                     IVATQMLESMTSSRLPTRAEATDVANAIIDGADCVMLSAESAMGRFPEESVAMLAKIA
                     TFTECHRPQPTLEAIQEMCEQDPIDSLGEMGSLVVERTLKTVPFEALFIPTRTGVTVR
                     MISRFKPSSWIIAAGTEHPTVRPLLFSYGVYPIEIEEEPECWTSYAAEWLK"
BASE COUNT         2289 a         2092 c         2537 g         2274 t
ORIGIN      
        1 ttggcggagg acaggggaag cggcttttcc cgcttacgag ttaccgttcc aaacccgcgg
       61 ttccgatcgg ggggaagtac cggttggtgg atattccgat ttcgaacgcg atcaattcgg
      121 ggttgaagcg aatctatgtg ttgacccagt ttaattcagc ttcgctccat cggcacatcc
      181 accggactta tccttacgaa tcgtttacca aaaccagtat tgaacttctc gccgcggaac
      241 agacggtaga caataaagac tggtttcaag ggaccgcgga cgcggttcgt aaatgtattc
      301 ctcattacca catggaaggc gtggatactt ttctgattct ttcgggcgat catctttacc
      361 agatgaacta cggggagatt ctcgggtttc accagatgca aaaagcggat gttacggtgg
      421 cgaccattcc cgtaccggag cgtctcatca aggggtttgg aatcatgaag ttgaccaaag
      481 acgggcgaat caccgaattt cttgagaaac cgtcgaaaga tgatgacccc tcctccttca
      541 tcatgcccaa aactctttgt ggcctccatc aggtcaaatc gaaaaagccg atgtatctgg
      601 cgtcgatggg aatttatgtg tttcggcggg aggctcttct ggaggcgctg aaggggaacg
      661 agaaagattt tggcaaagaa gtgattccga aggcgatcga aaattacaag gtgtttggtt
      721 atgtgcatac gggctactgg cgtgacgtcg ggacgatcgg gagtttttac gaggccagca
      781 ttgagctgac gcatcaaaac ccgccgttca gttttttgat tcctgatgga cgtgttttta
      841 cgcgcccccg atttttaccg ccttctaaaa tcattgacgc ccagcttgat cacgcgctga
      901 tgtcggaagg ttgcattctc acgggagcca agattgagca ttcggtcgta ggcctgcgat
      961 ccattgtggg gcacgggacg gtgattcgga ggagcgtctt gatgggaaat gaccactacg
     1021 aatggccgaa gccaggtgaa gacaggacga ttggtatcgg gaagaattgt ctgattgaaa
     1081 attgcattct cgataagaac gtggggatag gggataacgt tcaaatcacc aacagccgcg
     1141 gcagaaaaga agaagatgga gaaaattatt atatcaggga tggtatcgtg ataattccca
     1201 aagggggcta tattccggcg gggacgaaaa tttgagtatg aaaggtctcc tctttttttt
     1261 gacggcattt ttcatggtgt tttcttcagg gatgctttgt cgcgcgttac ccgcagacac
     1321 ggcttatggg gatgccattg aagatggcgg ccagggttcg gtgacaagcg cccagtcgac
     1381 tctggcatct ttatttggtg agaaggccgt catatcgata ccgccgcaaa ttgcccgtga
     1441 cgccacgatc gcgatacctt catttgattc gagggagctc gaaatcgaaa aaaatgatct
     1501 ttgggaaaga aattaaagcc tgaagttcaa gatcggcggg ctaatcagaa acacatgagt
     1561 aactgtgggg cgcccccgca ttttcgtttt tagcagaaca ctccatctct tgataacttt
     1621 cttaagctct cgcaagggca gtgtctcgca atcgtcttcc agtttgggaa cttaatccta
     1681 ataattcgct cgctcaaacc gtataaaagc gaacgcggca aaaaggatgt ttccgcccca
     1741 ggcgctcagg aaggggatga atttccctga ttttccgatg gcgatactga ccgctccgaa
     1801 gacgtgataa gcaaaaacga gagcggcgca gatgagaacg ttgatggcga tggatttgcg
     1861 ggtgtgaatt gaagccatga aggggatggc aatcagcatc atgacgaggc ctttccacgg
     1921 ggccgcaagc cgatcataga gatcaacctc ttccgaatag acggcgattc cgttttcttt
     1981 gagtttattg atggtgtatt ggagttcgtg gtaggagaga taaatacttt cgcttgtcgc
     2041 cataatcaaa tcccgcggag tgaccttgat gtccgggtaa atcttttctg ggatggtgac
     2101 cggactcccg agaacggctc cctggacgtc ggtctggtat tccgtgattc ccctgaaagt
     2161 ccacgctcct tcggcaaatg taccttcctt ggcaaaaacc tttcgggttg tgtgatgggt
     2221 tttggggtca agccaaagga tgatggcgtc tgccacctcc cggttttggg gtgtaaacgc
     2281 cctgaaataa taaaggacat tccctcccga gtagtaagtg acgtttttga gaaccttgcg
     2341 atcacccgaa gcctttttct tttctatgtg aatttcaagg agctcgttag cagttttgta
     2401 agtcgtggga acgattcggt cggtcacgag gaatgttaca attccgatca ggattgccag
     2461 aaagatgaga ggccggacaa tcgtcatgat tttcagcccc gcggctttca tggcaatgag
     2521 ctcgttatgg gccccgaggt ttacgaggag aaaaatcgtg ccgagccaca cggaccacgc
     2581 aatggtttgg ctgaaggcca tgggtatgag gcaaaggtaa taacggagta tgacaaggat
     2641 tggtgtcttg tttctcagaa gctcgtccag attatcaaaa agatccgcta tcaggatcag
     2701 gaaaacaagc gtgaccgtac agaagataat agggatgagt aactccctta cgagatatcg
     2761 gtccagtacc ttcaaaattg cccccttacg gcggttaaat gaccccttgt gtgttttttt
     2821 cgagggcgcc atcctgtcta cgacccaaag ggtcggaaac cggagaaaag ataagtgatt
     2881 tataaaaccg gttaaacgga agttctatac tatctgcgcg cgatggtaat ctcaacattt
     2941 tttaatgcgc gggcagttaa tccctgaaat caatctgatg aacttgggtt ttagaggatg
     3001 ggggagtgct ttatggatgt tcgtctgatg agcttttttc tttttttatc ggaagattag
     3061 ttttggcgag cgcgaagaga tccactcgtt ttgctccagc cttcttcaat gttctcgcgg
     3121 cttcgctggc tgtagcgccc gttgtgagca catcgtccac gagcagaaac gatttcccct
     3181 cggtctgttt cgcggatgag accttgaaaa cgccgtagat attgataacg cgctcttccc
     3241 gggtcatgga ggtttgtgat gggatgaagt atttcttttt cagaagattg ctccagatcg
     3301 gcagtttaaa aaaaggttgc aagagatccg ccagaatctg tgactgatta aactgccgtt
     3361 gaatgcgttt aaccctgttg agaggaatgg ggatgagcgc gttgtagacg tattcggaag
     3421 cgatggacgt cgcgagtttt gcggccgggt ttttaaaggc cttgagaaga taatcctttc
     3481 gtctgtattt gaccgcctgc aggagttcac gcaaaggtgt cacgtaagcg tagacggccc
     3541 acgcgtgatc caggttttca aactgttcat ctaccaaaga cttttcgagg ggataggcca
     3601 gcccctcgag gttttgttcg caggtttcgc aaagaaggtt ttcgtcgagg gccaaaggtt
     3661 ttccgcaaaa ctcgcaatgt gcgggataga ggaggcggat gagagattct ttgaaatggc
     3721 gcattgaatt attataataa atcattttta ttttgctata tatttataga tttttttaac
     3781 aaaatccctc ttccaaaaaa caaaacctgt gttacaatcc cgacatctgc gttctctccg
     3841 tggcgccagc tactggaaaa tacaagaatg caggtagcct cattccttga gcgagcaaat
     3901 ataagttttt tgtgagtttg tcattccacc aagaatgcaa ctcgcgcttg tttgcccccc
     3961 ccaaaaaaac aggaagagga atctccaagg aggaaatttc agtatgcaat cccaattcgc
     4021 agagctttac gaaaagtctt ttcagtccgt tgttccgggt gatgttgtga ccggaaccat
     4081 cattgaggtt cgtcaaaaag aagtcgtggt tgatattggt tacaaggctg agggtgtttt
     4141 gccttttgat gaatttacgg atcctcaatc gcttgccgtc gggcaggaag tcgaggtgct
     4201 ttttgaaggt tttgacgaca gggaaggcac ggctcttctt tcaaagcgca aagcggatcg
     4261 ccagaaaacc tggaacgaca ttttgtccaa tgccgaggaa ggcgcgatcg tcgagggacg
     4321 gatctctcgc aaagttcgcg gtggttttat ggttgatatc gggatggaag catttctccc
     4381 ggcttcgctc gtggatattc gccccgtgcg aaaccaggac gcgtttgtcg gtcttcagag
     4441 taaatttttg gtcgtaaaga tcaatcataa acgtaaaaac gttgtggtgt cacgcaagga
     4501 tcttctcgaa aaaagccggc aggaagcgcg ttcggaaaaa ctcaacgagc ttcaggttgg
     4561 acaaattgtt catggaaagg tgaaaaacat tactgatttc ggagtgttca ttgacatcgg
     4621 aaacctcgat ggccttctcc acattacgga catgagctgg ggacggattt cacatccttc
     4681 agaccttgtg aaaattgggg atgaaatcga cgtggccgtg atttcgatcg acaaggaaaa
     4741 acagaaaatt tcccttggtc tcaagcaaaa atcatcggat ccttgggaga cggttgaaga
     4801 tcgttatgcg atcggcaacc tggtccacgg ccgggtggtc aatattctcc cgtacggcgc
     4861 gttcgtggaa ctggagcccg ggattgaggg tcttgttcac atcagtgagc tctcctggac
     4921 caagagagtg acccatccga gcgaagttct ttcgatcgga cagcagttgg acgttgtgat
     4981 cctgagtctt gacacgacgg ccaaaaagat ctcccttgga gctcgccagg cgcaggaaaa
     5041 cccttgggat aaagtggctg acaaatacca ggttggatcc cgtgttcagg gaaccattcg
     5101 caaccttacg gattatggcg cgtttgtcga actggaaccg gggattgaag ggctcttgca
     5161 cgtttcggat ctttcctgga cccataaagt ggcgaaacct tcggatctcc tccagaaggg
     5221 tgagaccatg gaagtgatgg ttctgaatgt tgataccgaa gcgaagaaaa tttcactcgg
     5281 gctcaagcaa atgcaagatg atccctggaa cgaattgacc aaggacattc tgacgggcct
     5341 tgagattcag gggaagatca cgagagttgt caacttcgga gtttttgtcg aattggacaa
     5401 tggcctcgaa ggtcttatcc atgtctccga acttcctgaa aaaagtggag atgatctcgt
     5461 caagctctat aaggtggggg aaactattcg ctgtagtgtt ctgcatgtgg atcatgacgg
     5521 gcgaaaaatc gcgctaacct gcaaaaacga gtcgatttcc gcgtaaaagt gcttacctcg
     5581 aacgggcaaa atcggcgagt tgtcatcacg ggaatcggag tcatttctcc gattggatcc
     5641 ggaaaagcgg ctttttggga ttctctttca aaaggcaaaa gtggcatatc gccgatttcc
     5701 tcgttcgaca cttccgcgtt taccactaag tttgggggcg agatcaagga ttttgatccg
     5761 tccgcccaca tccttcccaa aaagctcaaa cgtatggatc gtaccactca gcttgccgta
     5821 gtggcctcca agatggcggt tgaggacgcg gggctaaact tccaggaaga tggttaccga
     5881 gaacggaccg gagtgattat cgggacggct ttggcgggcg tgggatacat tcttgagcaa
     5941 cacgacatcc ttcgtgaacg cggtccgatg cggcttaacc cgtttaccgc gctagcgtcc
     6001 tttccagacg cttgcgccgg gaacgtgtcc atcgaacttg gagtaacggg gccaagcttt
     6061 tctctggcga ccgcttgctc atcggcctca gatgcttgcg gctacgcctt tgacgctatc
     6121 cgaagcggtc gccttgattt tctgatcatg gggggagcgg aagcgacgat ttttccgggg
     6181 attatggcgt ctttttgcgt tgcccgggcg ctctccacgc ggaatgatca gcccgaaaaa
     6241 gcatcacgcc cttttagcgc ggatcgtgat ggttttgtgc tgggtgaggg cgcgggcatg
     6301 tttgtggtcg aagaatacga acgcgcccaa aaacgcgggg cccatattta tggcgaaatc
     6361 ctgggccatg gtatgacctg tgatgcttat cacatgacga tccctgaccc tgagggcaaa
     6421 gaggcgggac gggcgatgag tctcgctttg agtgaggcgg gcctgaggcc agatgaaatt
     6481 gattacatta atgcccacgg gacctcgact cccctgaacg ataaaaccga aacgatgatt
     6541 atcaaaaaag tttttggaga tcacgcgcga aagctcccga ttagttccac caaatccatg
     6601 atcggccatt taattggggc ggcgggaagc gtcgagctga tcgccacgtt gcttgccatg
     6661 gaaaatcagg tgattcctcc cacgattaat tacggctcca gggatcctga atgtgatctc
     6721 gattatgtcc cgaatgaggc gcgccctgcc aaaatccgaa tagccatgaa aaactctttt
     6781 ggatttggcg ggaaaaattc gattttaatt ttaaagaaag tttgatgcga atagcctgag
     6841 aagcgcgaaa gttcgccctc caacacttcg catgctgtac tccgagtgcc gaacttgata
     6901 taattcccgc catgtcgaac atcattcatc ccacggcaat catcggaagc aatgtttctt
     6961 taggtgacga gaattcaatc gggccatgcg tcaccataga aaatggagtc aagcttggcc
     7021 ggcgtaaccg gatatttcag gggacgttta ttggcgcggg taccgagttg ggagatgaca
     7081 acgagatcca catgaacgcc gtgattggcc acacccccca ggatattgct tacaagaatg
     7141 aagataccgc gacaaagatc gggaacaaaa atgtcatccg cgaatttgtc actattcacc
     7201 ggggtaccaa ggccggcact tcaaccgtga tcgggcatga aaactttatt atggcttatt
     7261 gccatatcgc gcataactgt tgtctcggca accgtatcat tatggttaat caggcctcgc
     7321 taaccggtca ttgcgtggtg gaagaccgtg cgtttctttc cggcatgacg ggatttcatc
     7381 agtttacgcg aattggcatg cttgccatgg tgagcgcgct ctcggcgttc aacaaagaca
     7441 tccctccttt tgtcatttgc ggtggacggc cgggtgtggc tcaggggatc aacgtggttg
     7501 ggatgcgtcg agccggtatc ggacccgagg tgcggaaaga aatcaaagaa tcctatcgcc
     7561 tcttgtatcg ttcggggctt aatacgactc aggcgattga agccatcaaa aaaagtttca
     7621 agagcccgga ggttgcgcac ttcgtcaaat ttatcgaaac atccaggcgt ggcattattg
     7681 acgggtcggg agccgtggcc gatacgattg ttccccggaa gaatcagggc gcgggagccg
     7741 gagaatcggc cgatgatgat ctgatcgcgt ctcccgacgt ttgacctccc ggacgggaca
     7801 aatttccgga cgcgacaggt aagaccttca tcaaaaaaaa tcaatgaaaa ggacgagata
     7861 agatggctgt acttccagga cataaaacaa aaattgtcgc aacgatcggg ccggcatctg
     7921 agtcaaggga gatgctggag cgtctgatca acgcgggcat gaatattgcc cggcttaact
     7981 tttcccacgg agactttccg gctcacggca aggtgattcg ccgaatccgt gaagcggaaa
     8041 aggccaccgg aaaaaaagtc gcgattatgg cggatctttc cggtcccaaa atgcggctgg
     8101 gttccataag cccggagccc gttgagctca aggcggggga cacgttcatc ctgacttcgg
     8161 atgatattat cggtaattcc cagcgggttt cgatgagctt taaatctctc cccaaggtcg
     8221 tgaaatccgg cgacaaactt tttctcaatg acgggcttgt ccagctggtg gttgaagatg
     8281 tgtcagggaa tgatgtccgg tgcaaggtgc ttgtgggcgg agaactccgt tcgaggaaag
     8341 ggctcaatct tccggggatc catcttggta ttgccgcgtt tacggaacgg gatcgggaag
     8401 gccttgagta tgcgctcgcg aatggcgtgg atgccataag tcagtcgttt gttgaaaatg
     8461 cgggagacat tgagattgtt cggcaagcgg cgatccggat gggaaagaat ccgtttatca
     8521 tcgcgaaaat tgaacgggcc ggggccctgg atcaacttga tgaaattttg aaagcggccg
     8581 acggcattat ggtggcacgg ggagacctcg gagttgaagt cccgatcgaa caaattgcga
     8641 tcacgcagaa aaaagtgatc gcaaaggcca acctggccgg aaagcccgtg attgtcgcga
     8701 cgcaaatgct ggagtcgatg acctcaagcc ggcttccaac tcgggcagaa gcgacggatg
     8761 ttgccaatgc catcatcgac ggggcggatt gcgtgatgct ctcggcggaa tccgcgatgg
     8821 gaagattccc cgaggaatca gtcgccatgt tggcgaaaat cgcgactttt acggaatgtc
     8881 atcgtcccca gccaacgctg gaggcgatac aggagatgtg cgagcaagat ccgatcgatt
     8941 cacttgggga aatggggtca ctcgttgtgg aacgcacgct taaaacggtc ccgttcgagg
     9001 ctctgtttat tcccacccgg acgggtgtaa cggttcggat gatctcgagg tttaaaccat
     9061 cttcctggat catcgcggcc ggcacggaac acccgacggt gcggccgctt cttttttcct
     9121 atggagttta tccgatagaa atagaggaag aaccagagtg ttggacttcg tacgcggctg
     9181 agtggttgaa aa
//
LOCUS       DAOSIO010000049         9108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_7233_length_9108_cov_4.452778, whole genome shotgun sequence.
ACCESSION   DAOSIO010000049 DAOSIO010000000
VERSION     DAOSIO010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 9108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9108
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7233_length_9108_cov_4.452778"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PK590_03050"
     CDS             complement(<1..906)
                     /locus_tag="PK590_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOG23608.1"
                     /translation="MNYDVNTFDSVAIRIASPETIRKWSKGEVRKPETINYRTLKPEK
                     DGLFCERIFGPTKDYECACGKYKRIKHKGITCDRCGVEVTQARVRRERMGHIDLVTPV
                     SHIWFFKTMPSRIGNLLDLSVRALEKILYYEEYIVTDPKQTPLKARQLLTEEEFHKAQ
                     EQYGLDAFDAMMGAEAIQKLLSEIDLDKLTQKYQRQVKVSKSKQSRARAVKILKIVEA
                     FRKSGNKPEWMIMKVIPVIPPDLRPLVALDGGRFATSDLNDLYRRVINRNNRLRKLLD
                     LKAPDVIIRNEKRMLQEAVDALFDNG"
     gene            complement(903..4682)
                     /gene="rpoB"
                     /locus_tag="PK590_03055"
     CDS             complement(903..4682)
                     /gene="rpoB"
                     /locus_tag="PK590_03055"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOG23609.1"
                     /translation="MMPLIKRKSFTKIQDTMELPNLVEIQIQSYEEFLQSNRSGRRRK
                     RQGLESAFADCFPIESFDGTCRLEYEGYTLGKPKYSIVECQKRGMTYAAPMKVKLRLV
                     RKGVAKEQEVYIGELPLMTETGTFIINGAERVIVSQLHRSPGASLEEAIHPSGKRIYS
                     VRIIPYRGAWLEFESDVNDVLYAYIDRRRKILATALLRALGYSSTYDILKELYPVEKV
                     KSVDRSTLKDIEDEYLAEDILHPESKEPILLAGSKLSKENIELLLANNVTSFSIARVT
                     KDDLCLINTIERDPYRNSVDAQMAIYRRIRPGDPPTEASAKDLIQKLFFDQQRYDLGV
                     VGRFMLNKKLGLPPEMKQTTLRRDDVIRVVGMLLKLRAGDVHVDDIDHLGNRRVRSVG
                     ELLQNQFRVGLARMKRSCVERMSIYDLDAAMPHNLINPKLISAAIKDFFGRSQLSQFM
                     DQTNPLAELTHKRRLSALGPGGLSRERAGFEVRDVHPSHYGRVCPIETPEGPNIGLIA
                     SLSTFARVNDIGFLESPYRKVVNGRVTEKIDFLTADEEDKYVIAQANAPIDKTGRFLS
                     DQTSCRFRGDFPKKKPEEIDYMDVSPRQIVSVAAALIPFLEHDDANRALMGSNMQRQA
                     VPLLTTEAPLVGTGMERHVAKDSGAVLTAKSRGTVTAVSSEEIVVDDFVYKLKKFKRS
                     NAGTCINQRPLVQVGDKIKRGDLIADGAGTQGGDLALGRNVLVAFMPWRGYNFEDAII
                     VSEKLCKQDTYTSLHIEEFDIEARDTKLGNEEITRDIPNVSEESLKDLDEEGIVRIGA
                     EVKPGDILVGKITPKSEIELSPEEKLLRAIFGEKAGDVRDASLTVPPGVEGIVVDVKV
                     FARKESAAKTKEERRQENKDINEIKERYTDRIELLKRERLNKVSDMVIGKKLSEDLLD
                     DILGEVLIKAGHVITKSDLKKLESCDWDSLRIDDDPDLEESIKKIARVWGDEIDELVA
                     ERTREIDRVKKGDELPPGVIKKVVVYVCSKRKISVGDKMAGRHGNKGVIAKILPEEDM
                     PFMEDGTSVDIILNPLGVPSRMNVGQILETHLGWAAKVLGLHVETPVFSGATEKEIKA
                     MMKEANLPPDGKVTLIDGCTGDPFDSKVTVGYIYMLKLAHLADDKIHARSIGPYSLVT
                     QQPLGGKAQFGGQRFGEMEVWALEAYGAAFTLQELLTVKSDDVVGRTRVYEAIVKGEP
                     ALHAGTPESFNVLVKELQALGLDIVPERVGGAKLSKTETKAEFDAREEAKEKLKV"
     gene            complement(4730..5146)
                     /gene="rplL"
                     /locus_tag="PK590_03060"
     CDS             complement(4730..5146)
                     /gene="rplL"
                     /locus_tag="PK590_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOG23610.1"
                     /translation="MTEATETKQISEKLQGILTSIETLTVLELADLVKALEEKFGVSA
                     AAAVPVAMAMPGGAGAAGAAPAEEQTSFTVVLASAGDKKINVIKEIRTVTSLGLKEAK
                     DLVEGAPKTIKEGASKEEADKIKKLLEAQGAKVEIK"
     gene            complement(5186..5722)
                     /gene="rplJ"
                     /locus_tag="PK590_03065"
     CDS             complement(5186..5722)
                     /gene="rplJ"
                     /locus_tag="PK590_03065"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOG23611.1"
                     /translation="MPSFEKELMFQEIVKEFEKSPYAFFSNFQNISVSEISEVRKNLE
                     KVSRRSLVIKHSMARKLFQKLNVTTAEKFLTGSVIVTFGEKDPQDISKKIVDFAKAND
                     KWKASGVIFEGQVHGEDFVKQLATLPSRKELMTQVVIRVKSPLAGLVNVLGGLTRNLV
                     VALGEIKKKRETSEGATA"
     gene            complement(5727..6413)
                     /gene="rplA"
                     /locus_tag="PK590_03070"
     CDS             complement(5727..6413)
                     /gene="rplA"
                     /locus_tag="PK590_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOG23612.1"
                     /translation="MMRSKRYQKKASLVEPEKAYSLQEAVQILKKVEPGKSDETISLN
                     FQLGIKAEAGDENVRGTVSLPHGSGRSVRVICFTKGETVKEAEEAGADIVGGEDLVEK
                     VKNGFLDFDVVVAHPDMMREVSKLGRVLGPKGLMPTPKTGTVTPQIAKAVKELKAGRI
                     EFKSDKTAGLHAVCGKLSFSEEAILENARAVIKAVRDSKPQISKGEYLRSVTISATHG
                     PGIKLSTGAL"
     gene            complement(6422..6847)
                     /gene="rplK"
                     /locus_tag="PK590_03075"
     CDS             complement(6422..6847)
                     /gene="rplK"
                     /locus_tag="PK590_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005021374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOG23613.1"
                     /translation="MAKEIQVKIKLQIPCGQANPAPPIGPALGQHGVNIMEFCKQFNE
                     RTKDRPGTILPVLLTVYKDKSFTFILKNPPIAAAIKKAVGLAKASSEPGRQMIGKLTR
                     EQAIEIAKAKLPDLNTNKVESAVKIVEGVARSMGIEVLK"
     gene            complement(6851..7384)
                     /gene="nusG"
                     /locus_tag="PK590_03080"
     CDS             complement(6851..7384)
                     /gene="nusG"
                     /locus_tag="PK590_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOG23614.1"
                     /translation="MADVKWYVIHTQTGVESKARASLISRAETEGLSHCIHDVLIPTE
                     KVSEVKAGKKRISERKFFPGYILVKMELTDESWYLVKNTPGISGFVGSGKKPLPLSER
                     EVGAIIEEQEEKMTKPKPKVEFSEGESVRVKEGSFANFNGVIEQINPDRGKLRVTVTI
                     FGRPTPVELEYWQVEKV"
     gene            complement(7389..7574)
                     /gene="secE"
                     /locus_tag="PK590_03085"
     CDS             complement(7389..7574)
                     /gene="secE"
                     /locus_tag="PK590_03085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOG23615.1"
                     /translation="MFQKTGNFLQQTKEELAKVTWPTWNELWQATVVVIIATFITAFF
                     IAVCDFFISNLIRLLIG"
     gene            complement(7605..7678)
                     /locus_tag="PK590_03090"
     tRNA            complement(7605..7678)
                     /locus_tag="PK590_03090"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7642..7644),aa:Trp,seq:cca)
     gene            complement(7848..7997)
                     /gene="rpmG"
                     /locus_tag="PK590_03095"
     CDS             complement(7848..7997)
                     /gene="rpmG"
                     /locus_tag="PK590_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOG23616.1"
                     /translation="MQEIISLVSTCDCKQTYWTRKNKKKNPDKLERKKYCPGCRQRVL
                     FREKK"
     gene            complement(8140..>9108)
                     /gene="tuf"
                     /locus_tag="PK590_03100"
     CDS             complement(8140..>9108)
                     /gene="tuf"
                     /locus_tag="PK590_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOG23617.1"
                     /translation="HYAHVDCPGHADYIKNMITGAAQMDGAILVVSAADGPMPQTREH
                     ILLARQVGVPAIVVFLNKCDLVDDAELLDLVELEVRDLLKKYDFPGDTTPIIRGSSLP
                     AAQNPTDEKAAQCIMDLVKALDDFIPEPKRDVEKPFLMPVEDVFSISGRGTVGTGRIE
                     RGIIKVNDEVEIIGLKETSMKSVVTGVEMFRKLLDEGRAGDNVGLLLRGIEKEQLERG
                     MVIAKPGSVTPHKKFKAQVYILSKEEGGRHTPFFKGYRPQFYFRTTDVTGIVTLPAGV
                     EMVMPGDNVSIEAELITPVAMEKELRFAIREGGKTVGAGVVSEIIA"
BASE COUNT         2248 a         2538 c         1916 g         2406 t
ORIGIN      
        1 gccattgtca aaaagggcat caaccgcctc ctgcaacatg cgtttttcat tccggatgat
       61 cacatcaggc gccttaagat ccaggagttt ccggagacgg ttattacgat taataacccg
      121 ccgataaaga tcgttcaagt cactggtcgc gaaccgccca ccatcgagcg ccaccaaagg
      181 tcgaagatct ggtggaatca ccggaatgac cttcatgatc atccactccg gcttattacc
      241 cgacttgcga aaagcctcga cgatcttgag aattttcacc gcccgcgcgc gagattgctt
      301 ggatttggaa actttcacct gccgttgata tttctgagtc aacttatcca ggtcaatttc
      361 ggaaagcaac ttttggatcg cctcggcccc catcatggca tcgaaggcat ccagtccata
      421 ttgctcctgc gctttgtgga attcttcttc cgttagaagc tggcgggcct tgagaggagt
      481 ttgtttggga tcggtgacaa tatactcctc gtaataaagg atcttttcga gtgcgcggac
      541 acttaaatca agaagatttc cgatccgcga cggcatcgtc ttgaaaaacc aaatatgaga
      601 cacaggtgtt accaaatcaa tgtgccccat ccgctcacga cgcacgcggg cctgcgtcac
      661 ttccacgcca catcgatcgc aggtaatccc tttatgttta atgcgcttgt acttgccgca
      721 ggcacactca taatcttttg taggcccaaa aatacgttca cagaaaagac catctttttc
      781 gggcttgagt gttctgtagt tgatagtctc cggcttcctc acttcacctt tggaccattt
      841 tcggatcgtt tcgggtgaag caattcggat ggcaacagaa tcgaaggtgt taacgtcgta
      901 gttcatactt tcagcttctc cttagcctct tctctggcat caaactcggc tttggtttcc
      961 gtcttggaaa gcttggcgcc tccaacccgc tctggaacaa tatccagacc cagcgcctga
     1021 agctctttga caagcacgtt aaaggattcc ggtgtccccg cgtgtagcgc gggctcacct
     1081 ttcacaatag cttcataaac ccttgtacgg ccaaccacat catctgattt gacagtcaac
     1141 aactcctgga gcgtaaatgc ggcgccataa gcctcaagcg cccacacttc catttcccca
     1201 aaacgctgtc caccaaattg cgcttttcca cccaacggtt gctgggtcac aagcgaatac
     1261 ggcccgattg agcgggcatg aattttatca tccgccaaat gtgccagttt taacatgtag
     1321 atataaccca cggttacctt gctatcaaaa ggatcaccgg tacaaccatc tatcaacgtc
     1381 acttttccgt ccggcggaag gttcgcctcc ttcatcatcg ctttgatttc tttttcggtc
     1441 gcgccggaaa acaccggtgt ttccacatgc aaaccgagta ccttcgcggc ccatccaaga
     1501 tgggtctcaa gaatttgccc aacgttcatt cgggaaggca caccaagcgg gttcaaaata
     1561 atatccacgg aggtcccgtc ttccataaat ggcatatctt cttcggggag gatttttgca
     1621 atcacaccct tgttcccgtg acgacctgcc attttgtccc cgaccgatat ctttcgcttc
     1681 gagcaaacat agaccacgac ctttttaatc acaccaggag ggagctcatc tccctttttg
     1741 acgcggtcaa tttcgcgggt tcgctccgcc acaagttcat cgatctcatc accccagacg
     1801 cgcgcaatct tcttgatact ttcctcaaga tcggggtcgt catcaatccg gagagaatcc
     1861 caatcgcagc tttcaagctt tttgaggtca cttttcgtga ttacatgacc cgccttaata
     1921 agtacttcgc ccaagatatc gtccagaaga tcctcggaaa gtttttttcc gatcaccata
     1981 tccgagacct tgttcaacct ttcgcgtttg agcaattcaa tgcgatcggt ataccgctct
     2041 ttaatctcgt tgatgtcctt attttcctgc ctccgctctt ccttggtctt cgcggcggat
     2101 tccttgcgcg cgaatacctt gacgtcaacc acaatcccct ccacccccgg aggaaccgtc
     2161 agggaggcat cacggacatc tcctgctttt tcgccaaaaa tcgcccggag caatttttct
     2221 tccggagaaa gctcaatctc ggatttaggc gtaattttac caacgagaat atcacccggt
     2281 tttacttccg ccccgatcct aacaattcct tcttcatcga gatctttcag agattcttcg
     2341 ctaacattcg ggatgtcccg ggtaatctct tcgttaccca acttcgtgtc ccgagcctcg
     2401 atgtcaaact cttcaatatg gagcgaggta taagtgtcct gtttgcaaag cttttcgctg
     2461 acgatgatcg cgtcttcaaa gttgtaaccg cgccacggca taaatgccac caagacatta
     2521 cgaccaagcg ccaaatcgcc accctgtgtc ccggcgccat ccgcgatcaa atcgccacgt
     2581 ttaattttat cgccaacctg gacaaggggc cgctgattga tacaggtgcc ggcattggag
     2641 cgtttaaact ttttgagctt gtacacaaaa tcgtcgacta caatttcttc ggaagaaacg
     2701 gcggtaacgg tcccacgcga cttggcggta agcaccgcgc ctgaatcctt cgcgacatgc
     2761 cgttccatac ccgtgccgac caggggcgct tcagtggtga gaagaggcac cgcctggcgt
     2821 tgcatattcg accccataag agccctgttc gcgtcatcat gttcaagaaa cgggataaga
     2881 gccgccgcca ccgaaacaat ctgtcgcgga gaaacgtcca tgtaatcaat ttcttcgggc
     2941 tttttcttgg gaaaatcgcc gcgaaaccgg caggaagttt gatcactcaa aaatcgaccc
     3001 gttttgtcaa tcggcgcatt ggcttgcgcg attacatatt tgtcttcttc gtcggccgtc
     3061 aagaaatcta ttttttcggt gaccctgccg ttaaccactt ttcggtaagg actctcaaga
     3121 aaaccgatgt cattaacacg ggcaaaagtg ctcaaggacg caatcagacc gatgttgggt
     3181 ccttccggtg tttcgatcgg acaaacacgg ccatagtgcg acggatgaac gtcgcgaact
     3241 tcaaaacccg cacgttcgcg cgaaagccca ccgggaccca gcgcagaaag acgccgttta
     3301 tgggtcaact cagccaacgg gttcgtttga tccatgaact gcgaaagctg actgcggcca
     3361 aaaaagtcct tgatcgccgc cgaaatcaat ttgggattga tcagattatg cggcatcgcg
     3421 gcatccagat cataaatcga catacgttca acgcatgatc gtttcatgcg ggccaatcca
     3481 acccgaaact gattttgaag aagctcgccc accgaacgga cgcgacgatt gccaagatgg
     3541 tcaatatcat caacgtgaac atcgcccgcg cgaagcttga gcaacattcc gaccaccctg
     3601 atcacatcgt cccgacgcaa cgtagtttgc ttcatctctg gcggaaggcc caatttctta
     3661 ttgagcataa aacgtcccac aactccaagg tcataacgtt gctggtcaaa gaaaagcttt
     3721 tggatcagat ctttcgcgct tgcttcggtt ggcgggtcac ccgggcggat ccgacgataa
     3781 atcgccattt gcgcgtcaac tgagttccgg taagggtctc tttcgatcgt attaatcaaa
     3841 caaaggtcat ctttggtaac tcgggcgatc gaaaacgacg tgacgttatt ggcgagtagc
     3901 aactcgatgt tttcttttga aagcttgctt ccggcgagta aaatcggctc cttagactcc
     3961 ggatgtaaaa tatcctcggc cagatattcg tcttcgatat ccttaagcgt cgacctgtca
     4021 acgcttttaa ctttctcaac aggataaagt tccttgagaa tatcgtaagt tgaggaatat
     4081 ccgagcgcgc gcaaaagcgc cgtcgcgagg atcttgcgac gacgatcaat ataggcataa
     4141 aggacatcat tgacgtccga ttcgaactcg agccacgcac cgcgataagg aatgatccga
     4201 accgaataaa ttcgtttgcc acttggatga atcgcttctt ccagggaagc tccgggggag
     4261 cgatgcaact gactcacgat cacgcgttcg gcaccgttga taataaacgt accagtttca
     4321 gtcatgagcg gaagctcacc aatatagact tcctgctctt tggctacacc tttccggacc
     4381 aaacgcagtt ttaccttcat cggagccgca taagtcatac cccgcttttg gcattccaca
     4441 atggaatatt tgggcttacc aagcgtgtac ccctcgtatt caagacgaca ggtcccgtca
     4501 aaactttcga tcggaaaaca atccgcgaac gccgattcca gaccttgccg tttacgacgg
     4561 cggccactac gatttgactg cagaaactcc tcataagact gaatctggat ctcaaccaga
     4621 ttcggaagtt ccatggtatc ctgaattttg gtaaaacttt tcctcttgat caaaggcatc
     4681 attagaagaa tgccctcctt agaatggtgt aacctgaacg gacaggtgct tacttgattt
     4741 caaccttggc gccctgggct tcaagaagct tcttgatctt gtcagcttct tccttgctcg
     4801 ccccttcctt aatggttttc ggcgcgcctt caacaagatc cttggcttct ttcaggccga
     4861 ggctcgtcac ggtacgaatt tccttgatca cgttgatttt cttgtcgccc gcacttgcca
     4921 aaacgacggt aaaggaagtc tgctcttcag caggcgccgc accggccgcg ccagctcctc
     4981 ccggcatcgc catcgccacg ggaacggccg ccgccgcgct gacgccaaat ttttcctcga
     5041 gtgcctttac aagatccgca agctcaagaa ccgtcaaagt ctcgatcgac gtcaagatac
     5101 cctggagttt ttcagaaatt tgttttgttt cagttgcttc cgtcatgatg tgttgcctcc
     5161 ttgattgttt gaataaaaac tgtggttacg ctgtagcgcc ttcggacgtt tcccgtttct
     5221 tttttatctc accaagcgcc acgacaagat ttcgggtgag accgcccaaa acattgacta
     5281 aaccggccaa gggcgacttg actcggataa caacctgcgt catgagttct ttacgacttg
     5341 gaagcgtggc aagctgcttt acaaaatctt cgccatggac ctggccttcg aaaataactc
     5401 cgctggcttt ccacttgtca ttcgctttcg cgaaatcaac aatttttttt gagatatcct
     5461 gcggatcttt ttcaccaaac gtcacaatga cggagcccgt caaaaacttc tcagcggttg
     5521 tcacattcag tttttgaaaa agcttcctcg ccatggagtg ttttatgacg agagaacgcc
     5581 ttgaaacttt ctccagattt tttcgaactt cggaaatttc agaaactgaa atgttctgaa
     5641 aattcgaaaa aaaagcgtaa gggctttttt caaactcttt cacaatctcc tgaaacatta
     5701 attctttttc gaacgatggc atcgaattac aacgctcctg tactgagctt gatcccgggc
     5761 ccatgagtcg ccgaaatcgt cacgctcctg agatattcgc ccttgctgat ttgaggcttt
     5821 gaatcgcgaa ccgctttaat taccgcgcgg gcattttcaa gaattgcttc ctcgctaaat
     5881 gagagttttc cgcacaccgc gtgaagcccc gccgtcttat cacttttaaa ttcaatgcgt
     5941 cccgccttta gttctttaac cgctttcgcg atttgcggcg tcaccgttcc ggtcttgggt
     6001 gtcggcatca atcccttcgg cccgagaaca cggcccagct tactgacctc acgcatcata
     6061 tcgggatgcg ccaccacaac gtcaaaatca agaaacccat ttttcacttt ttccacaaga
     6121 tcttccccgc caacgatatc agcgccagct tcttccgcct ccttgacggt ttcacctttc
     6181 gtgaaacaaa taacacgtac cgagcgacca cttccgtgag gaagacttac cgttccgcga
     6241 acattttcgt cccccgcctc agccttaatt ccaagctgaa aattgagtga aatagtttca
     6301 tcggatttac ccggttccac tttctttaaa atctgcacgg cttcctgcaa cgaatacgcc
     6361 ttctcaggtt caaccaggct tgcttttttc tgatatcgtt tacttcgcat catcaatcct
     6421 tttatttaag aacttcaatc cccatgcttc gggccacgcc ttcgacaatc ttcacggcgc
     6481 tttcaacttt gtttgtgttg aggtcaggca acttggcttt tgcgatttca atcgcctgtt
     6541 cccgcgtaag ctttccgatc atttgacgcc ccggctccga agaagcctta gccaacccaa
     6601 ccgctttctt gatcgccgcc gcgatcggag ggtttttcaa aataaacgtg aaagacttat
     6661 ctttatagac tgttaacaac accggaagaa tcgtccccgg cctgtctttc gtgcgctcat
     6721 taaactgctt acaaaactcc ataatgttca caccatgttg acccagcgcg ggaccaattg
     6781 gcggcgcagg atttgcctgc ccacatggga tctgcaactt gatttttacc tgtatttctt
     6841 tagccatttt ctaaaccttt tcaacttgcc aatactctag ttcaacgggt gtcggtcggc
     6901 caaatatggt gaccgtaacg cgaagcttcc cgcggtccgg attaatttgt tcaataactc
     6961 cgttaaaatt agcaaaagaa ccctctttaa cgcgaacgct ttctccctcg gaaaactcaa
     7021 ctttgggctt cggcttcgtc atcttttctt cctgctcttc gatgatcgct ccgacttccc
     7081 tctccgagag cgggagaggt tttttccctg atccaacaaa cccggaaatt cccggggtgt
     7141 ttttcacaag ataccaactc tcgtccgtca attccatctt gacgaggatg tacccaggaa
     7201 agaactttct ttcactgatt cttttttttc cggccttcac ttcgctgact ttttcggtgg
     7261 gaattagcac atcatgaata caatgtgaaa gcccttcagt ttccgcccgg ctgatgagac
     7321 tggctcgcgc tttcgactca acgcccgtct gcgtatgtat gacgtaccac tttacatctg
     7381 ccataaaatc aaccaatcaa aaggcggatt aaattagaaa taaaaaaatc gcaaacggca
     7441 ataaaaaaag ccgtaatgaa tgttgcgata atcactacga ctgtagcctg ccaaagctca
     7501 ttccacgtgg gccaagtgac tttagccaac tcttctttcg tctgctgtag aaagtttccc
     7561 gttttttgaa acatataaag cttatattcc ttttctgata accgcaggcc tggcaggact
     7621 cgaacctgca acatgcggtt ttggagaccg ccgctctacc aattggagct acaggccttt
     7681 atcccgttag aagcttcaca cttccgattg aaggacaatt tctttaccct caacacgtca
     7741 caagtcttaa actgctttta ggcgttaaac aaacagatac gagccaaacc cacgatggcc
     7801 cgcacatctc acaaacaaaa cggcaacaaa gccgatccaa aaaactttta tttcttttct
     7861 ctaaaaagaa cgcgctgacg acaccccgga caatactttt tccgctcaag tttgtcggga
     7921 ttctttttct tgttttttct cgtccaatag gtctgcttgc aatcacaagt cgacactagg
     7981 gaaataattt cctgcatcta aaattaaaac cttttccttt ggtcaaaaaa tacgctatgg
     8041 gctcaataca ggtataggag tttcagggac gctaaaacac cctaaaaatc gttgaagatt
     8101 cgcctggact tcgctaaaaa atacgggatt aacgagacgt taagcgatga tctcgctgac
     8161 cacacccgcg ccaaccgtct taccaccctc gcggatagca aaccggagtt ctttttccat
     8221 ggccaccggt gtaatcagtt ccgcttcaat cgagacattg tcacccggca ttaccatttc
     8281 tacgccagcc ggcaacgtca cgatacccgt cacgtctgtg gtgcggaagt aaaactgcgg
     8341 ccgatacccc ttgaaaaatg gagtatgacg tcctccttct tccttgctca aaatatagac
     8401 ctgcgcctta aatttcttgt gaggcgttac gcttcccggt ttagcaataa ccatcccgcg
     8461 ctcaagctgt tctttttcaa taccgcgaag gagaagtcca acgttatcgc cggcacgtcc
     8521 ctcatcaagg agctttcgaa acatctctac gcctgtcacc acggacttca tggaagtttc
     8581 tttgagacca atgatttcaa cttcatcgtt aaccttgatg attccgcgtt caatcctccc
     8641 cgtccccacg gtaccgcggc cagaaatcga aaacacgtcc tcaacaggca tcaaaaacgg
     8701 cttttcaaca tcccgcttcg gctcggggat aaaatcatcc agtgccttaa caagatccat
     8761 aatgcactgt gccgcttttt catccgtcgg attttgagcc gccggcaaac tcgaaccacg
     8821 aataatcggg gtcgtatctc ccggaaaatc atatttttta agaagatcgc gaacctcaag
     8881 ctcaacaaga tcaagaagtt cagcgtcatc gacaagatca catttattca aaaaaaccac
     8941 aatcgcggga acgccgacct ggcgggcaag caaaatgtgc tcacgggtct gaggcattgg
     9001 gccatccgcc gcggaaacga caagaatggc gccatccatc tgggccgcgc ccgtaatcat
     9061 gtttttaatg tagtccgcgt gaccggggca gtcaacgtgc gcgtaatg
//
LOCUS       DAOSIO010000050         8933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_7387_length_8933_cov_4.118833, whole genome shotgun sequence.
ACCESSION   DAOSIO010000050 DAOSIO010000000
VERSION     DAOSIO010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 8933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8933
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7387_length_8933_cov_4.118833"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PK590_03105"
     CDS             complement(<1..276)
                     /locus_tag="PK590_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23618.1"
                     /translation="MSHFLDLNKFWGGQPSPVGLMVGAFMVELLFKRCQDQPIDEFPD
                     VRNKSNGLEDPGFVVVTKVIGLAPCIHKNDRLRKNILSEPASVGWECS"
     gene            889..1308
                     /locus_tag="PK590_03110"
     CDS             889..1308
                     /locus_tag="PK590_03110"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HOG23619.1"
                     /translation="MSLKKRYLSLLTLALLVAAGYFLSTQITGAPEARVPEYSDPARP
                     VKTRIGSHFVIVLDSNPTTGYGWQLGESFDKTMFEVTEIRYEPSNGKRLAGAGGKQTW
                     MFRALCAGNAVIDFEYLRPWEKDIPPVKKVRFAVTVR"
     gene            complement(1352..2041)
                     /locus_tag="PK590_03115"
     CDS             complement(1352..2041)
                     /locus_tag="PK590_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG23620.1"
                     /translation="MANETILIIEDEKNIVELVKFNLEQEGFRVLAASRGNTGLEMAL
                     REKPALLILDLMLPEITGIEICKILRQNPKTKTLPIIMLTARGTEADKVLGLELGADD
                     YITKPFSPRELAARIKAILRRSREQPESEMMRAGPLELDISRHELRLSGKKIGITAKE
                     FQLLRALMGSCGRVLTREVLLEKVWGCDNSINIETRTVDMHIGQLRKKLGKESGRIVT
                     IKSVGYRFDND"
     gene            2302..>8933
                     /locus_tag="PK590_03120"
     CDS             2302..>8933
                     /locus_tag="PK590_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23621.1"
                     /translation="MGKFMTAIRKSDNTVKGLIRFVAAFTLQAFVLSTLFFTSPAEIF
                     ANEFSILPPSTFSDLFLKFPSRIGKFDRLFSPLSGNQSVFFLHIKDAHASREAQQNIA
                     RILTRLRDRKQLDLVLIEGAAGTPMPGRLDIFKDAKANTSYKNDLLKAGLMNGAAYFI
                     ASEQDVPSGGLEDPRMYFNHAQIFKRVVEARRNSGNFFDTIEAVMLNTFKNILRMDVF
                     LFLKEWRLQHQKEMSWGRYIPFLIRSAEKFLNLDLSDYRAQILWPNLVRFRHLNALEE
                     VIRPEAVKSEFRRLSAVLSRDRDPALEQTMTLLFVAVNNPVGMIQETPDFRRYAEVLV
                     DACQRSGVLLQEYPNLVRYLAFRIFRSEIESEGMLAETERLEEAVFDKLFTNEKMRKV
                     ITAYKDFYTLRRAFRLELNRAESNRFLAMDAEKKIRQFSKIAPGYDFPAEEVIKDVET
                     VKKFYQLAQKRDDAFVTQIRNRLSEARVLPDRPFFIAIVTGGYHAEGLENWLRSEGLP
                     FVGIQPSFKDEPNTERYEKILLGDYQTVDALATAQGAILPDDTARKLMGRADFTALRD
                     AEARFATRYSSVTAPVVEPAKISRTSRPVGRSEIRLKNLGARLGAGRRRFIVLTAIAV
                     AGLLSGCASLSHTELKKTLREAETYELPRRVVAVPAVIHVDSQSLLQRILENDPKVKK
                     AEIEIAIKEIEKDRLKGNWSITTRVAWVDGKPVFSGGLGASIPHVIEGGTTGHGVGSL
                     ATLAVDLAGSLSSILRGEPMLARALSEKAVQAAELNHQQIIGERYAYYAGIAIEIRKL
                     QRLYETSQLAIRDMEAALKVAEARAEVPELEKTKIRDLLGEWRIRAGEYDSLLKEKKS
                     LLLNLYAPGGVATTKFEIGLRENTLPSDWRFSPTMIEQLRNDATHKNGNQLPPNRKLA
                     FAYKGREAAVVLRELEKARGRFSLGFAGFSFADSLASPSGMSSSEKADIGSAWDRGLD
                     RRTKDKNAAGISGEISFGKAAKVDERISSRRVEVTDAVLADVQRTVEDTVSDLVHQIG
                     RNQDKARILRGELERLEREMQRARQVEVAGRRFVTEDKLAGSISAKWGHRLELIKASA
                     ETEQAILKLEINAGVLGGFSWRTNRSELRAVQTFREGNVEFAMGAIDQLAPAGAQRAE
                     IRISQRPQGKHKSFISKFKIFILSMALFASLATSVFAGNGATDLKGSGARNDAVVELK
                     PGSSGTELGHAYEMYDDFTARILNTIPEGSNKKALQRFAEILRVTIVSEETLRQAQQL
                     FVREVVSKETAETVKVAAVSGPSAVGAGLVKVAATDSRIGDEMSAGSEANRHEERMLP
                     ATDSSGNLGAERSAREVENASSRSLGSGALGPDVSRGKASEVKRGESVTKGKQTTLSS
                     EPVVGDLQPAAVTPQVFLLGPGSAGSTQLPAPVPHNQSTDISPVMRILLSGASGTPDG
                     ASQNTNTDVTRSPSDSRLDAATTLPVTPQKIAALAESAGTGAGQSLSSKPETVAVAGD
                     DLPVSSSVLAAPEHEMVDSHILEGKEKVHPVLSNVTAALTGIPEYQVKEGDVVPRGRT
                     VARLSDPENAGRIRALETEVALMDAQLADPSLSVAEFLQLRIRKESLEKELRERTYQE
                     ELRDLRSSHPIKIVRLTHGQVNQGEVLAHGVQLDAGQIVLRIPIEQADFDSAELKVNG
                     ETVLVLRYGLSDFDPLNRQFLLTLDFISPSGAELGPQCDYTLKLGRLHKQEAADPSLA
                     RNKPFHITVSPRESVPVSVPVAPGIPGGIFRVLVPEGSIVSAGTPVGIIDASGYQQEH
                     LNAAQGLRKLVGEVESANRQFAAFSPEELRKMNADANLQIASLEGVDPKIVVRAPVSG
                     RVEGLIGIDGQVVVPGQATGLRILNSLVSLGSASLTDSDHLIDVPKGSVREGERVRVR
                     TPLGDVLDGTVFKVMPLADENGLWLSDRDAVVLQVEDPEMRLGDNMPVEVWTGIPAGE
                     HGSVPASHPAVAELPLRYESDMPPAVFYRLLRHPDQELSSHSVLAGEGSFRQILSSWK
                     FFFKSPKGLAAMGKKVANRVFLLLGGAIILWLAGRFVRSAAQKGRWRHSKARLHDLMT
                     ITSELQYRLTIRFEQQGRPQDKELLEHVIGPINEWARMLDEEGELDHEKRTTILIKAQ
                     ELASNKHLVFSGIKFWDIDMWNSQIGRDLAIIFGILTVLCVLTARRITEQVQNAKTPV
                     ELHELQVFQQRLVDYGDIFN"
BASE COUNT         2081 a         2174 c         2496 g         2182 t
ORIGIN      
        1 cgagcattcc cagcctacgc tcgcaggttc ggagaggata ttctttcgca ggcgatcgtt
       61 cttatgtatg caaggagcca atcctataac cttcgttact acgacaaatc cgggatcttc
      121 aagcccgttc gatttgtttc ttacatctgg aaattcatcg atgggttgat cctggcatcg
      181 cttaaaaagg agctctacca tgaacgctcc caccatcaac ccgactgggg aaggttgccc
      241 gccccagaat ttgttgaggt ctaaaaagtg cgacataatg tcccaccccc gcggggctat
      301 ttcaccgatg tccgcccttt tgggccggac atttcttatc ctattcagca atatatagtt
      361 gctgaaatgt cgtcccaaaa agtccggaca aaacggacat gtttttgccc tgtttcataa
      421 tctggacgtg cttgggcaag agggaatgtc ggatgtatcg tgatctctga atcggtctac
      481 acatgaatac tcatacatat gtcctatgct acaatacggc cgcttttaga gcgtatgaca
      541 cgcaaaggac gtaagcgcgt cacattcgtt tttggaatgg gagccaactc aaataggttc
      601 cttgcgagtc cctatgggac atccgggctc acattcgttt ttggaatggg agccaattca
      661 aataggttcc ttgtaagtcc ctgtgggaca tccgggctca cattcgtttt tggtccatac
      721 cggacagaga taaaatcttc cgagggcatc gggtttttcc atttccaggc ctgtgtgatt
      781 ccgcaatttt cttttaaaat cgctccttta ttttcaaaat accctctggt ttcagaaaac
      841 tggtttcaat ctattacagt gcacggcgtc cggtttggtg aggtgtttat gagtctaaaa
      901 aaacggtatc tgtctttgct gacactcgcg ctgttggtcg cggcgggata tttcctttca
      961 acacaaatca ctggtgcacc ggaggcccgg gttcccgaat acagcgatcc tgcccgcccc
     1021 gtcaaaacgc ggatcgggag tcacttcgtg atcgttctcg actcgaatcc cacgaccggc
     1081 tacggatggc agttgggcga atcattcgac aagacgatgt tcgaggtcac ggagatccgc
     1141 tacgagccgt caaacggcaa gcgtcttgcc ggcgcgggtg gcaaacaaac gtggatgttc
     1201 cgcgcgcttt gcgcggggaa cgccgtcatt gattttgaat acctgcgccc ctgggagaaa
     1261 gatattccgc ccgtgaagaa ggttcgcttt gccgtgaccg tacgctagat cgagtttgtg
     1321 ggctgggact ttctgcccaa aagcaatatg gctaatcgtt gtcaaaacgg tagccgacgc
     1381 ttttgatcgt cacgatccgt cccgattcct tgccgagttt tttgcggagt tgtccgatgt
     1441 gcatatcgac cgttcgcgtt tcgatattga ttgaattgtc gcagccccac accttctcga
     1501 ggagtacttc ccgcgtaaga acgcggccgc atgatcccat aagcgctcgc agtaactgga
     1561 attcttttgc cgtgatcccg atctttttgc cgctcaagcg aagttcgtgt ctggaaatgt
     1621 cgagttccag agggcccgcc cgcatcattt cagattccgg ttgttcccga gaacgtcgca
     1681 aaatggcttt gatacgggcg gcaagttctc ttgggctgaa gggtttggtg atgtaatcat
     1741 ccgcgccaag ttcaaggccg agaactttgt ccgcctcggt tcccctggcc gtcagcatga
     1801 tgatgggaag ggtctttgtc ttcggatttt ggcgaagaat cttgcagatc tcgatcccgg
     1861 ttatttcggg gagcatgagg tcaagaataa gaagcgccgg tttttccctg agtgccattt
     1921 ccagaccggt attgccccgg gaagcggcga gaacgcggaa tccctcctgc tcgagattga
     1981 attttacaag ctcgacgata ttcttttcgt cttcaataat gagaatggtt tcgtttgcca
     2041 tttttagacg gatgttagca caaaaaaaca ttctgaatgg ccccctccag ccttttttac
     2101 atacttttta caatcctgtg tttccggggt attgggtgag gggagttttc aaagggagta
     2161 cccggttacc cgtctaggag gtcgacatat ttgatctcgt gattttaccg aattattaca
     2221 agatcctcac agtgagatta cgctttatgt ctaaacttac catgtgtttt gagatgtttc
     2281 aaaagttgtt aaaaagtatt cgtgggaaaa tttatgacgg ccattcgaaa aagcgacaat
     2341 acggtgaaag gattgatacg atttgtggcc gcttttaccc tccaggcttt tgtcctttcg
     2401 accctttttt tcacttcacc cgctgaaata ttcgcgaatg aattttccat attgcctccg
     2461 tcaacatttt cggacttgtt tttaaaattt ccgtcccgga taggaaagtt cgaccgtctc
     2521 ttcagcccgt tatccggaaa tcagtcggtt tttttccttc acatcaaaga tgcccatgct
     2581 tctcgggaag cccagcaaaa tatcgcccgc atcctcaccc ggctcaggga caggaaacag
     2641 ctggatctcg tcctgattga gggtgctgcg ggaacaccga tgcccgggcg gctggacata
     2701 ttcaaagacg cgaaagcgaa cacatcttat aaaaatgatt tgctcaaggc cggtctgatg
     2761 aacggcgcgg cgtattttat tgcctcggaa caagatgttc cttctggggg tctcgaggat
     2821 cctcggatgt attttaacca cgcgcagatt ttcaagcgcg tcgtggaagc gcggcgaaac
     2881 tccggaaatt tttttgatac gatcgaagcg gtgatgctta acacgttcaa aaacattctc
     2941 aggatggatg tcttcctgtt tcttaaggag tggaggcttc agcaccaaaa ggaaatgtcc
     3001 tgggggcgtt atataccgtt tttgatccga agcgctgaaa agtttttgaa cctcgatctt
     3061 tcggattatc gcgcgcaaat tttatggccg aatctggttc ggttccggca tttgaacgcg
     3121 ctcgaagaag tcatccgtcc ggaggcggta aaaagtgaat ttcgccgtct ttcagctgtc
     3181 ttgtccaggg atcgggaccc cgcattggag cagaccatga cgcttttgtt cgtggctgta
     3241 aacaatccgg tcggcatgat ccaggaaaca cccgatttca ggcggtacgc ggaggtcctt
     3301 gtggatgctt gccagcggag cggtgtttta ttgcaagagt acccgaacct ggtccgctat
     3361 ctcgccttcc ggattttccg ttcggagatc gaatcggaag gcatgctcgc cgaaacggag
     3421 cgactggaag aggccgtttt cgacaagctc tttacaaacg aaaaaatgag gaaggtgata
     3481 acggcataca aggattttta tacgctcagg cgcgccttca ggctggaatt gaaccgggct
     3541 gaatccaatc ggttcctggc gatggatgcg gagaaaaaaa tccgtcaatt ctcaaaaatc
     3601 gcgcccggat acgactttcc ggcagaggaa gtgatcaaag acgttgaaac ggtcaaaaaa
     3661 ttttatcaat tggcccaaaa gcgcgatgat gcttttgtga cgcaaataag aaaccggctc
     3721 agcgaggcgc gggttttacc ggaccggcct ttttttattg ccatagtcac cggaggatat
     3781 cacgccgagg gtcttgagaa ctggctccga tcagaagggc ttccgtttgt cggtattcag
     3841 ccttcgttca aggacgagcc caatacggag cgttacgaaa aaattctttt gggagattac
     3901 cagacggtcg atgcgcttgc gaccgcccag ggcgctatct tgccggatga tacagccagg
     3961 aaacttatgg gccgggccga ttttactgcg cttcgggatg ccgaagcccg gttcgcgact
     4021 cgttacagct ccgtgacagc tccggttgtt gagccagcaa aaatttcccg gacttctcgg
     4081 cccgttgggc gttctgaaat caggttgaaa aatctgggcg cgagattggg cgcggggaga
     4141 cgccgtttta tagttttaac cgcgatagcc gttgcggggc ttctctcggg atgcgcgagt
     4201 ctttctcaca ccgaactcaa aaagacgctc cgtgaagcgg aaacctatga gttgccccgg
     4261 cgagttgtcg cggtgccagc ggttatccat gtcgattctc aatcattgct tcaaaggatt
     4321 cttgaaaacg atcccaaagt taaaaaagcc gaaatcgaga tcgcgatcaa ggagatcgaa
     4381 aaagaccggt tgaaaggcaa ctggtccatt acaaccaggg tcgcttgggt agacggaaag
     4441 cccgttttca gcggggggtt gggcgcctcc ataccccatg tgattgaagg cggcacaacc
     4501 ggtcatggcg tcggaagcct cgcgacgctg gcagtcgatc tcgcgggttc gctgagcagt
     4561 attttgcgtg gagagccgat gctggccagg gcgctttctg aaaaggcggt acaggccgcg
     4621 gagcttaacc atcagcagat catcggggag cgttacgcgt attacgccgg aatcgcgatt
     4681 gaaattcgaa aacttcagag gctatacgag acttcccagc tggcgattcg ggatatggag
     4741 gcggccctga aggtcgcgga agcaagggcc gaagttccgg agctcgaaaa gacgaagatc
     4801 cgggatctgt tgggcgaatg gcggatccgg gcgggggaat acgactcttt gcttaaggaa
     4861 aagaaatcgt tactgcttaa tctttacgcg ccgggaggcg tggcgaccac aaaattcgag
     4921 atcgggctga gagaaaacac attgccttct gactggcgtt tctcgcccac gatgattgaa
     4981 caattgcgta atgacgcgac gcataaaaac ggaaaccagc ttccgccaaa ccgcaaattg
     5041 gcattcgctt ataaagggcg ggaggcggca gtggttcttc gggaacttga gaaagcccgg
     5101 ggccgtttca gcctcgggtt tgccgggttc agtttcgccg attctctggc aagtccgtcc
     5161 ggcatgagtt cctctgaaaa agcggatatc ggtagcgcgt gggatcgggg cctggatcgt
     5221 cgcaccaagg acaagaatgc cgcggggatc agcggggaaa tttctttcgg gaaggcggca
     5281 aaggtcgacg agcgaatatc ttcccggcgc gtggaagtta cggacgccgt tctcgcggat
     5341 gttcaacgta ccgtggaaga cacggtatca gaccttgttc accagatcgg aaggaatcag
     5401 gacaaggcaa ggatcctcag gggagagctt gaacgccttg agcgcgaaat gcagcgcgcc
     5461 cggcaggtgg aggtcgcggg gcgccggttt gttaccgagg acaagcttgc cgggtcaata
     5521 tcagcaaaat ggggtcatcg gctcgaactt atcaaggcca gcgccgaaac agagcaggcg
     5581 attttaaaac ttgaaataaa cgcgggggta cttggtggtt tttcttggcg gacgaatcgc
     5641 tcggagctcc gggccgttca gacattccgc gaaggcaatg tggagtttgc catgggggcc
     5701 atcgatcaac ttgccccggc cggagctcaa cgggctgaaa ttcgaatcag tcaacggccg
     5761 caaggtaaac acaaatcctt tatatcaaag ttcaaaattt ttattttgtc catggcgctt
     5821 tttgcttctc tcgccaccag tgtttttgcc ggtaacggag cgacggatct caaaggttct
     5881 ggggccagga atgatgcggt tgttgaactt aaaccgggga gttccggaac tgaattgggt
     5941 catgcttacg aaatgtatga cgactttacg gcacgtattc tcaatacgat tcccgaagga
     6001 agtaacaaaa aagcgctcca aaggttcgcc gagatacttc gggtgacgat tgtttctgaa
     6061 gaaacgcttc ggcaggcaca acagctgttt gtccgcgaag ttgtgtctaa agaaaccgcc
     6121 gaaacggtga aggtggccgc ggtttctgga ccgtcagcgg ttggagcggg tctggtcaag
     6181 gtcgcggcaa cggactcccg tattggtgat gaaatgtcag cgggaagtga ggcgaaccgt
     6241 catgaagaaa ggatgttgcc cgcgactgat tcgtcgggaa atcttggagc tgaaagatct
     6301 gctcgagagg tagagaatgc ttcctcgcgg tcgctcggat caggggctct aggaccagat
     6361 gtttctcgcg gaaaagcttc agaagtaaag cgaggagaat ctgtcacaaa aggaaagcag
     6421 acaaccctgt cttcagagcc ggtagttggg gatcttcagc ctgctgcggt taccccccag
     6481 gtttttctcc ttggtcctgg ttcggcgggt tcgacccaat taccggctcc tgttccgcat
     6541 aatcaatcga ctgatatttc gcccgtcatg cgaatattac tcagtggtgc ttcaggaaca
     6601 ccggacggag cctctcaaaa taccaacaca gacgttaccc gttcgccatc ggatagtagg
     6661 ctcgatgcag ccacaactct tcctgtaacg ccacaaaaaa tcgcggcgct cgcggaatct
     6721 gccggcactg gcgctggtca gtcactttct tcgaaaccgg aaacggttgc agttgccgga
     6781 gacgatttgc ccgtatcttc ctcggttctg gcggccccag aacacgagat ggtcgatagc
     6841 catattcttg aagggaagga aaaggttcat ccggtgcttt cgaatgttac ggcggcattg
     6901 accggcatac ccgaatatca ggttaaagaa ggggatgtgg ttccgcgggg tcggactgtc
     6961 gcgaggctct cggatcccga aaacgccgga cgcattcgtg ccttagagac cgaagtcgca
     7021 ttgatggatg ctcagttggc cgatccatct ttaagtgtcg cggaattcct tcaactgcgt
     7081 attcgaaaag aatccctgga aaaagagttg cgcgagagga cctaccagga agaacttcgg
     7141 gaccttcggt cctcccaccc gatcaagatc gtcagactaa cacatggaca ggtgaatcaa
     7201 ggtgaagttt tggcccacgg ggtacaactc gatgccgggc agattgtcct ccgaattccg
     7261 atcgaacagg cggattttga tagcgccgag cttaaggtga acggggaaac tgtcctggtc
     7321 ttacggtacg gtctgagtga ttttgatccc ttgaatcggc aatttctttt aacgctcgat
     7381 ttcatctcgc cttccggggc ggaattggga cctcaatgtg attacacgct caaactcgga
     7441 cggcttcata aacaggaagc cgcggatcct tctctggcgc ggaacaagcc ttttcatatc
     7501 acggtgtcgc ctcgtgagtc tgtgccggtg agcgttccgg tcgcgcccgg gatcccggga
     7561 ggaatttttc gcgtgcttgt tcctgaagga tcgatagttt ccgctggaac tccggtcggg
     7621 atcattgatg cttccggtta tcagcaggaa cacctgaacg cggcacaagg gctcagaaag
     7681 ctggtgggcg aagtcgaatc agccaatcgg cagttcgccg ctttttctcc ggaggagtta
     7741 cgcaagatga atgccgacgc gaaccttcag atagccagcc tcgaaggagt ggatcccaaa
     7801 attgtcgtgc gggccccggt gagcggccgc gttgaaggat tgattggaat tgatgggcaa
     7861 gttgttgttc ccggtcaggc gacgggactt cgtattttga attcactcgt ttctttaggt
     7921 agcgcgagct tgacggacag cgatcacttg atcgacgttc cgaaaggaag tgtgcgcgaa
     7981 ggggaaaggg tcagagtccg gacgccattg ggtgatgttt tggacggaac ggtttttaag
     8041 gtgatgccgc ttgccgatga aaacggtcta tggctttccg accgggacgc ggttgtgctt
     8101 caagtcgagg atccggagat gcggttgggc gacaatatgc ctgtcgaggt ctggactgga
     8161 attcctgccg gtgagcatgg aagcgtgccc gcgtcccacc cggcggtggc ggaacttcct
     8221 ctccgctatg agagtgacat gcctcccgcc gtcttttacc ggttattgcg ccatcccgat
     8281 caggaacttt ccagtcattc ggtgttggcc ggtgaaggaa gcttccggca gatcctctcc
     8341 agctggaaat ttttctttaa gagtcccaaa gggctcgcgg ctatggggaa aaaggtggca
     8401 aacagagttt ttttactgct tggcggagcg attattctct ggctggcagg ccggtttgtg
     8461 cggagcgcgg cacaaaaggg ccggtggaga cattcgaagg cgagattgca tgatctgatg
     8521 accattacaa gcgaactcca gtatcgtctg acgatccgtt tcgagcaaca aggccggccg
     8581 caggacaaag aattgctcga gcatgtgatc ggtcccatca atgaatgggc caggatgctg
     8641 gatgaggagg gggaactgga ccatgaaaaa cgcacgacaa tcctgatcaa ggcccaggaa
     8701 ctggcatcca acaagcacct tgttttttca gggatcaaat tttgggacat tgatatgtgg
     8761 aacagccaga ttggcaggga tctggcgatc attttcggaa tattgacggt gctttgcgtt
     8821 ctgaccgcgc gcaggattac ggaacaggta caaaacgcga aaactcccgt ggaacttcat
     8881 gaactccagg tttttcagca acgccttgtt gattacgggg atattttcaa tga
//
LOCUS       DAOSIO010000051         8887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_7425_length_8887_cov_3.668478, whole genome shotgun sequence.
ACCESSION   DAOSIO010000051 DAOSIO010000000
VERSION     DAOSIO010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 8887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8887
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7425_length_8887_cov_3.668478"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4191
                     /locus_tag="PK590_03125"
     CDS             <1..4191
                     /locus_tag="PK590_03125"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOG23622.1"
                     /translation="LHEDGYRYTTLNAMKENAEGSVLFDFLLRDLRRRENAKRILFLA
                     RGAECLKVTARYVNWLFSPEIVQGALENFLFMLSQDTGGKLISDIKDLFRFSPKTYLL
                     GEEGEPDEFVESVSYDALERQAMEILEDLDRGKSRWTHVSRLYAREINPYDGDGKGLN
                     RFTQFFDKIYDWIKEAGIVDGLQPGETILVVDESSSGTYPWILKYVIETRSLADLAKR
                     LGKPESEIQPFRVIFQAGGRSNHDYFIENFPIFDYERVLGYGNFAVSAIADKGENMPH
                     PVKFGSVGSILEEATGQVIFESEEPDVLLGAYLNSMFIYNEVVRFYNYLNGLVRKKNK
                     TQKEWNLLLHYLTLKLPPEVEATPAALARASEFVLTERDIKMALVRKGILNELRDYAG
                     AVYCLSDPVDLPSVLALQELMPEARIILNDSFVPGGSELVKDVPEPGDERAVRDFLEK
                     KFGDIGKAHGFTQTVVSRGEAPGTYIVFLKKGKLSDFQRVEIEYRAGDFYQMEIPKGT
                     GVVYLNLPERDGVSLENPGLWEKLGVGLQGPGYALVASRFDGDSFEAPGFSKLADGVE
                     MGLFTNGVFQISRAEVRESPKSDFDRDFHRWIGNYWQELRRAYPDLPDSILKPTFYRM
                     YKMVRDEIAPLIKEDDRILEIGVGSGVIISMLAEEVKKRGIHNVRFIGTDIDPDVARI
                     ARRMTQKYGNVEVLPAGDLFDPVLGMKFDWVIWNPPWFQEQLINGQEYGPKMVDPGYG
                     VVRRFFREVPDYLTPSGRSAVIFPSKYFPKAASEFERDYVKVAGINPKFEIGIYISDL
                     MRGKREGWSGDPKISRASAPKEHDPKRPQRERYKREKLNKRSEVRDEGLVKAEEPAVS
                     ASMSPESVRSEMNSQGMVEAGWRKFVGRADAVRRAWNVSEKKETGGKQGVEKPFASEA
                     VVRSDALRSGHLCLVRGNFDKLTQGLRTALDAVRDEIPVFQRWDETDSLSSELKMLTE
                     LVRNISQHVRGKEGILAIQFDKDAKRLYVAAIDRGEGFSFLTKGARTQDAKEEFGISL
                     RADGYFEQSGINRVGAGTGMRDVVRQSERVLIRSRDTEWSEEDLRLQGQGVIPGIERK
                     DGLPGSLVCVSVNLEESMRIINRARQIREARKDARRTEIRWEEQSRKFLTELEERSEL
                     RKFVMSDGFSNAANIELGEFADPTPFVTSVRESLLNAMLPKAFAAETVDPVIAKIVPD
                     KMKALNATLEQVKGRLTIALDLGTLSGAMGQFMEIPVEGTAPVNEIGVTGVLDGGTRD
                     LLRTRGITVRTVEPKRNFISRNQTQVPYVTSNFSVETLSDIFDPILADQISTGDVSAQ
                     CYRDRFLIVAAIHLADIRRGGKAMSLSEIRDELLRRLNLKEKDALIHIRGRGFAVSSA
                     AVETYLRMQAEKSIQKAA"
     gene            4352..5758
                     /locus_tag="PK590_03130"
     CDS             4352..5758
                     /locus_tag="PK590_03130"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOG23623.1"
                     /translation="MEKLKIPLVILAILLIGAALLPGAGFSPQQVISVLVFLVILCGT
                     LFYWKFRLAFAFAGISILLGTKLIDVPHIIEFAGLDIILFLVGMMTIIGYLEENHFFE
                     YLVARAVDKVGERPYWLIAMLMVMAFFSAALVDEVTSILFMTSTLFHITRRYKINPAP
                     FLLMIVFATNIGSSATAVGNPIGVMIALRAKLTFLDFLRWATPVSVACLLVTVPLSFF
                     LFRKPIRQLGKNMKEEGNEPHELERVQYTRAGIRKCWLLFLGTVICLVLHHNIEEFLH
                     LEKNTLLIGTALFFGAVSIFLKGAEARDFFMRRVDWWTLTFFMSLFASVGTLKYVGVT
                     EHIAQGMIRIGSSGGFFLLNVFTAAICVLTAFMDNVLAVATFLPILADIQSIGVYIFP
                     FWWAMLFGGTMFGNATVIGSTANIVAMGMFEKETGQGIKFMDWLKPGLAVSAVTILVA
                     MALLYLQFPLMPDAPAHF"
     gene            complement(5830..6228)
                     /locus_tag="PK590_03135"
     CDS             complement(5830..6228)
                     /locus_tag="PK590_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23624.1"
                     /translation="MTYDTIRLLQFMICREFNDGPRDSYNAMSCLSNLHVPSYPCHLK
                     NLYAVTCWRKDRQFHKEVIEYELPNSPILKTAPMDIEPVTSSVLFRWHKHAFPADLVV
                     KEDSLLRIRVILDWKPLWETYIMIERGGTA"
     gene            6361..7773
                     /gene="lysS"
                     /locus_tag="PK590_03140"
     CDS             6361..7773
                     /gene="lysS"
                     /locus_tag="PK590_03140"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOG23625.1"
                     /translation="MSTSDYGARFLPKESIAGILADFQPGKKVRVAGRVMSRRDMGKS
                     TFSDLKDEGGRIQTYAKKDVLGEENYKAFKGLNLGDIIGIEGELFLSKTGEKTIKIEK
                     YERLSKIVQTLPEKWHGLKDIEIRYRRRYVDLIVNDDVRATFKKRSRIVSEIRKFLDG
                     RGFMEVETPMMQPLAGGARAKPFVTHHHALHTDLYLRVAPELYLKRLLVGGFEKVYEI
                     NRNFRNEGVSVRHNPEFTMMELYQAYADYSDMMNITEELVSHLVKMLYGTEEIPYGDR
                     TLNFKTPWKRVSFYGALQEATGMDFRTVNVREAAKKLRVEFSDTMEDVDILDEIFGEK
                     VEKELWDPTFVIDYPTVMTPLAKAKSDDPDLVYRFELFIARMEVANAFSELNDPVIQR
                     QRLEDQRELIGEEKEIDDDFLMALEYAMPPAGGLGIGIDRLVMLLTNQPSIRDVILFP
                     QLKPLKKAEEEPLAAENELA"
     gene            7770..>8887
                     /locus_tag="PK590_03145"
     CDS             7770..>8887
                     /locus_tag="PK590_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG23626.1"
                     /translation="MKFELFIAHRHLTRRRKSGFISLISLISIGGVAVGVMALIVVLS
                     VMTGFDTELKRKIVSVQPHLRIEKYQSWENSEEDMRKIRSLNISSLKSVARFIEKQAI
                     LRTDDSAQGVVVMGMDTENEDMSFYQKNIVSGTLGFQESVYTESKRSWWFKKKTQKSN
                     VPSVIIGDILARRLRVQVGDLLYLITPEPEKENPFLPLPMHVKTWPVIVRGIFHVGMS
                     DADANLALISLEAAQKFYHMDGKITGLGLRFFNVDDAEKWKWTLAGEFSNQYVLASWY
                     DMNQSFFQALKVEKFLLGILLLLIVVVAAFNIVSTLIMVAMEKTKDIGILRALGATKS
                     AVRRIFMLEGMSYGFWGMVIGVGLGVWCSLKINDLSDFL"
BASE COUNT         2142 a         1833 c         2571 g         2341 t
ORIGIN      
        1 ctgcatgagg acggatacag gtatacgacc ttgaatgcca tgaaggaaaa tgctgaggga
       61 agtgttctgt tcgatttttt gcttcgcgac ttacgccgcc gagaaaatgc caaacgaatt
      121 ctctttctgg ctcgcggcgc ggaatgctta aaagttaccg cacggtatgt gaattggttg
      181 ttttctcccg aaattgtgca gggggccctt gagaattttc tttttatgct ttcgcaggat
      241 accggaggga agcttattag tgacattaaa gatctattcc gattttctcc taaaacatac
      301 ctcctggggg aagagggcga gcctgatgaa tttgtggaaa gcgtttccta tgatgccctg
      361 gaaaggcaag cgatggaaat tcttgaggat ttggatcggg ggaagagccg ttggacgcat
      421 gtatcacgat tgtacgcgag ggaaattaac ccttatgacg gggatggaaa aggtcttaat
      481 cgatttactc aattttttga caaaatctat gactggatca aggaggcagg aattgtggac
      541 ggtcttcagc ccggggaaac aattctggtc gtggatgagt cttccagtgg aacttacccc
      601 tggattttga aatatgtgat tgaaacgcga agcctcgctg atttggcaaa acggctggga
      661 aaacccgaat cggagattca gcccttccgg gttatttttc aggcgggcgg tcgatccaat
      721 cacgactatt ttatcgaaaa ttttcctatt tttgactacg agagagtctt gggatacgga
      781 aactttgccg tctccgcaat tgccgataaa ggagaaaaca tgccgcatcc ggtaaagttt
      841 ggtagcgtcg gcagtatttt ggaggaggct acgggtcaag tgatctttga aagtgaagag
      901 cctgatgtgc ttttgggtgc ttatctgaat tcgatgttta tctacaatga agtggtgaga
      961 ttttataatt atctaaatgg tcttgtgcgc aaaaagaata aaacgcaaaa agagtggaac
     1021 ttgcttttac attacttgac gcttaagttg ccaccggaag tggaggctac ccccgcggca
     1081 ctcgcccgcg cttccgaatt tgtcttaacg gagcgtgata tcaagatggc gcttgtccgc
     1141 aaggggatct tgaacgagtt aagggattac gcgggagcgg tgtactgcct gtccgatccg
     1201 gttgatttgc cctcggttct cgcgttgcag gagctgatgc cggaagcgag gatcattctt
     1261 aatgattcgt ttgttccggg gggctcggag cttgtcaagg atgttcccga acccggagat
     1321 gaacgggcgg ttcgagattt tctggaaaaa aagtttgggg atataggcaa ggctcatggt
     1381 tttacccaaa cggtggtttc gcgtggtgag gcgcctggta cgtatattgt ttttctgaag
     1441 aagggtaagt tgtctgactt tcaacgggtt gagatcgaat accgggctgg tgatttttac
     1501 caaatggaaa ttcccaaggg gacaggagtc gtttatttga atcttccgga gcgcgacggt
     1561 gttagtttgg agaatcccgg gctctgggaa aagttggggg ttgggcttca gggacctgga
     1621 tatgcccttg ttgcttcgcg ttttgacggc gattcttttg aggcgccggg attctcgaag
     1681 ctggcggatg gcgtggagat gggattgttc accaacggtg tttttcagat aagccgcgct
     1741 gaggttcgag aatctccgaa atctgacttt gatagggatt ttcatcgatg gataggaaat
     1801 tactggcagg aattgagaag ggcttacccg gatttaccgg acagtatcct gaaaccgact
     1861 ttttatcgca tgtacaaaat ggttagggat gagatagcgc cattaatcaa ggaagatgac
     1921 cgaatccttg aaataggagt cgggagcggc gtcataattt ccatgttggc tgaagaggta
     1981 aaaaaacggg gtattcacaa tgtccggttt attgggacag acattgatcc ggatgtcgcc
     2041 cgaattgcgc gcaggatgac gcaaaagtac ggaaatgttg aagttcttcc ggccggagat
     2101 ttatttgatc ctgttttggg tatgaagttt gactgggtga tctggaaccc cccatggttt
     2161 caggaacagt tgattaatgg tcaggaatat gggccgaaga tggtagatcc agggtatgga
     2221 gtggtgcgtc gatttttcag agaagtgcct gattatttga ccccgtcggg tcgaagcgct
     2281 gttattttcc cgtccaagta tttcccgaaa gcggctagtg aattcgagcg tgattacgtc
     2341 aaggtggccg gcattaatcc gaaatttgag attggtatct atatctctga tcttatgagg
     2401 ggaaaacgag aagggtggag cggcgaccct aaaatatcac gagccagcgc acctaaggaa
     2461 cacgatccca aacgtcctca aagagagcga tacaaaaggg agaagttgaa caagcggtcg
     2521 gaggtgaggg atgagggctt ggtgaaagca gaagaacccg cagtaagcgc ctccatgtca
     2581 ccagaatctg tgcgttcaga gatgaacagt cagggtatgg tggaggccgg gtggcggaaa
     2641 ttcgttgggc gggcggatgc ggtccgaaga gcatggaatg tttcggaaaa gaaagagaca
     2701 ggaggaaaac aaggagttga aaaaccattc gcatcagaag ctgtcgtgag gtcggacgct
     2761 ttacggagtg ggcatctttg tctggtgcga ggtaattttg acaagttgac tcaggggctt
     2821 cgtaccgctc ttgacgcggt ccgggatgaa attccggtgt ttcaacggtg ggacgaaact
     2881 gattcactat cgagcgagct caaaatgctt actgagcttg ttcgcaatat ctctcaacat
     2941 gtccggggta aggaagggat tctggcgatt cagtttgata aggacgccaa acggttgtat
     3001 gtggcggcga ttgatcgagg tgagggtttt tccttcctga cgaagggcgc tagaacacaa
     3061 gacgctaaag aagagtttgg catttcgctt cgtgcggacg ggtattttga acaaagtgga
     3121 attaatcgtg ttggcgcagg aaccggaatg agagatgttg tccggcaaag tgaacgcgtt
     3181 ttaatacgca gtcgcgatac agaatggtct gaggaagatc ttaggttgca agggcaaggg
     3241 gtaattccgg gaattgaacg aaaggatggt ctgccgggct ctcttgtttg cgtgagcgtt
     3301 aatcttgagg aatccatgag gatcattaat agagcgagac aaatacgaga ggctcgtaag
     3361 gatgcccgcc gcacagaaat ccgatgggaa gagcagagcc gcaagttttt gacggagctt
     3421 gaggagcgtt ctgaactcag gaagtttgtg atgagtgatg gattcagtaa tgcggctaat
     3481 atagaacttg gcgagttcgc tgatccgacg ccgtttgtga cgagtgtccg cgagtcattg
     3541 ctgaatgcca tgcttccgaa ggcttttgcg gcggaaacgg tggatcctgt gattgcgaag
     3601 atcgttccgg ataaaatgaa ggctttgaac gcgacgcttg aacaggtgaa aggacgtttg
     3661 acgattgctt tggatcttgg aacgctttcc ggtgcgatgg gacagtttat ggaaattcct
     3721 gttgagggca cggcgccggt caatgaaata ggtgtgacag gagttttgga tggcggaaca
     3781 agggatctgc ttcggaccag agggattaca gtccgtactg ttgagccgaa acggaatttc
     3841 atttccagga atcaaaccca ggtaccctat gtgacctcaa atttttctgt cgaaacgctt
     3901 tccgatatat ttgatccgat cttggcggat cagatttcaa cgggtgatgt ttcggcgcag
     3961 tgctatcggg atcggttttt gattgtggca gcgatccatc tcgctgatat caggaggggc
     4021 ggaaaggcga tgagcttgtc cgagatccgg gacgaacttt tgcgccgtct caacctgaag
     4081 gagaaggacg cccttattca cattcgggga cggggatttg ctgtttcaag cgccgcggta
     4141 gagacttatc tccgaatgca agcggaaaaa tccatccaaa aggccgcgta aaaagcttca
     4201 atcaaagggc gcgcggcacc ttccctaatc gctccctgac aaattgctga aaaggctctt
     4261 cctcattaac cagccaaaaa gccaagaaga ttttctgtct ttataaaaaa ttttgataca
     4321 ataagcctcc aacagtgaaa ggattttcta aatggaaaaa cttaagattc ctctcgttat
     4381 tctcgcgatc cttttgatcg gggcggccct gctaccggga gccggttttt ctccgcaaca
     4441 ggtcatttca gttcttgttt ttctcgtgat tttgtgcggc actctttttt actggaagtt
     4501 tcgtctggcg tttgcgtttg ccggtatttc gattttgctg ggcactaaac tgattgatgt
     4561 cccgcacatt atcgagttcg cgggtcttga tatcatcctg tttctggttg ggatgatgac
     4621 gatcattgga tatctcgaag agaaccactt ctttgagtat ctcgtggccc gcgccgttga
     4681 caaggtgggg gagcgtcctt actggttgat tgccatgctc atggtgatgg cttttttttc
     4741 cgcggcgctg gtggatgagg tgacttccat tttattcatg acgtcgaccc tgtttcatat
     4801 tacccgccgt tacaaaatca atccggcccc ttttttactt atgatcgttt ttgccaccaa
     4861 catcggaagc agcgcgacgg cggttggtaa tccgatcgga gtgatgatcg cgcttcgcgc
     4921 caaactgacc ttcctggatt ttctgaggtg ggcgacacct gtgtccgtgg cgtgtctgct
     4981 ggtgacagtg cctctgtcct tttttctttt caggaagccg atccgtcagt tagggaaaaa
     5041 tatgaaagag gaggggaacg agcctcacga gcttgaacgt gtccagtata cgcgagcggg
     5101 gattcgtaag tgctggctac tttttttggg gacggtgatc tgtctcgttc tgcatcacaa
     5161 catcgaggag tttcttcatc tggagaagaa cacgcttttg atcggaacgg ccctgttctt
     5221 cggggcggtt tcaatttttt tgaaaggggc ggaggcgaga gatttcttca tgcggcgcgt
     5281 ggactggtgg acgctcactt tttttatgag tttgttcgcg tcggtcggga ccctcaaata
     5341 tgtcggtgtg acggagcata tcgcgcaggg gatgatccgg attggcagta gcggcggatt
     5401 ttttcttctg aatgttttta cggccgcgat ttgtgtgctg acggcgttta tggataacgt
     5461 tctggcggtg gcgacattcc tgccgatcct cgcggacatt caatccatcg gtgtctatat
     5521 tttcccgttt tggtgggcga tgctttttgg ggggacgatg ttcgggaacg cgacggtgat
     5581 cggaagtacg gccaacattg tcgccatggg aatgtttgag aaagaaaccg gccagggaat
     5641 caagtttatg gattggctca aaccgggcct ggcggtctcc gcggtgacga tccttgtggc
     5701 catggcgttg ttgtatctgc agtttcctct gatgcctgac gcgccggctc acttttaaat
     5761 tcgccagacg ccgaggctag aggaagtcag tttttacgcg ccgactcgcg gatttaagaa
     5821 aaacagtgat tacgccgttc cgccccgctc gatcataatg taggtttccc aaagcggttt
     5881 ccagtccagg attacccgga tccggagcag ggaatcttcc ttcacgacaa gatcagcggg
     5941 gaatgcgtgt ttgtgccagc gaaaaagaac gctggaagtg acgggttcaa tgtccatcgg
     6001 ggcggtttta aggatcgggc tgtttggcag ttcgtattca atgacttctt tgtgaaattg
     6061 cctgtccttt cgccagcagg tgacggcgta gagattttta agatgacatg gatacgaagg
     6121 gacgtgaaga ttgctgagac aggacatcgc gttgtaagaa tctcgcgggc cgtcattaaa
     6181 ttcgcggcaa atcatgaatt gaagcaggcg gatcgtgtcg taagtcatgc gacgcatcat
     6241 aattgaaacc ggtatccttt tcaagatttg aactcaattt gaccctgtct tgaaactaac
     6301 atataatgac acttccaatc gtcttaaatc attccgaatc aaggcataag gaaaaaattc
     6361 atgagtactt cagattatgg agcgaggttt ctcccgaaag agtcgatcgc cggcattctg
     6421 gctgatttcc agcccgggaa aaaggttcga gtggcgggcc gtgtgatgtc ccgccgggac
     6481 atgggaaaga gcactttctc agatctcaaa gatgaagggg ggcggattca aacctacgcc
     6541 aagaaagacg tattgggaga ggaaaattac aaggcgttca aggggttgaa tcttggcgac
     6601 atcattggta ttgagggtga gctttttctt tcgaaaacgg gagaaaagac gattaaaatc
     6661 gaaaaatacg aacgcctgtc aaagatcgtt caaacgcttc ccgaaaaatg gcacggtctg
     6721 aaggatatcg aaattcgcta tcggcggcgg tatgtcgatc tgatcgtcaa tgatgacgtg
     6781 cgcgcgacgt ttaaaaaaag aagccggatt gtgagcgaga tccgcaagtt tctggacggt
     6841 cgcggattca tggaagtgga aacgccgatg atgcagccgc tcgcgggggg cgctcgggca
     6901 aagcctttcg tcacgcacca tcacgcgtta cacacggatc tgtaccttcg cgtggcgccg
     6961 gaactttacc tgaaacgtct tctggtgggt ggatttgaaa aggtctacga gattaatcgt
     7021 aatttcagga acgagggggt ttcggtccgg cataatcccg aatttaccat gatggagctc
     7081 tatcaggcct acgcggatta ttcggacatg atgaacatca cggaagaact tgtctctcat
     7141 cttgtgaaga tgctttatgg aacggaggaa attccctacg gtgaccggac gctgaatttc
     7201 aagacgccgt ggaagcgcgt ttccttttat ggcgcccttc aggaagcgac cggcatggat
     7261 tttcgtaccg tcaacgtgcg ggaagccgcg aagaaacttc gcgtcgaatt ttccgacacg
     7321 atggaggacg ttgacattct cgacgagatt ttcggggaga aagtcgaaaa agaactttgg
     7381 gaccccacct ttgtgattga ttatccgacg gtgatgacgc cgctcgcgaa agccaaatct
     7441 gacgacccgg accttgttta ccgttttgaa ttatttatcg cgcgcatgga ggtggccaac
     7501 gccttttctg aactcaatga cccggtcatt cagaggcaac gcctggaaga tcagcgagag
     7561 ctgatcggcg aggaaaaaga aatcgacgat gacttcctga tggccctgga gtacgcgatg
     7621 ccaccggcgg ggggacttgg aattgggatt gaccggcttg tgatgcttct cacgaatcag
     7681 ccttcaatcc gcgatgtcat cctgttcccg cagttgaagc ccctgaagaa ggccgaagaa
     7741 gagccgctcg cggcggagaa tgaactggca tgaaattcga actttttatc gctcatcgac
     7801 atctgacccg gcgtcgcaaa agcggattta tttccctgat ttccctcatt tcgattggcg
     7861 gtgtggcggt tggtgtgatg gcgttgattg tggtgctttc ggtcatgacc ggatttgata
     7921 cggaactgaa gcgcaagatc gtgagtgtcc agccgcacct gcggattgaa aaatatcagt
     7981 cgtgggaaaa ctcggaagag gatatgcgga aaatccgatc cctcaatatc tctagtttga
     8041 agagtgtcgc ccggtttatt gaaaagcagg ccatcttgcg gacggatgat agcgcgcagg
     8101 gcgtggtagt gatggggatg gataccgaaa acgaggacat gtctttttat cagaaaaata
     8161 ttgtgtccgg gacgctgggt tttcaggaat cggtttatac ggaaagtaaa cgcagctggt
     8221 ggttcaagaa aaaaacacag aagtcgaacg tgcccagcgt gatcattggc gatattctcg
     8281 cgaggcggtt gcgtgtccag gtgggggatt tgctttacct gattacgccg gagcccgaaa
     8341 aggaaaatcc ttttttacct ctcccgatgc acgtcaaaac ctggcccgtg attgttcgtg
     8401 gaatctttca tgttggcatg tcggacgcgg atgccaatct tgccctgatc agtcttgaag
     8461 cggcccagaa gttttaccac atggatggga aaattacggg actgggtttg cgttttttca
     8521 acgtggatga cgcggaaaaa tggaagtgga ctttggccgg tgagttttcc aaccagtatg
     8581 tgctggcttc gtggtatgac atgaatcaaa gcttttttca ggcgctaaag gttgaaaaat
     8641 ttttattggg aattctgtta cttctgatcg tcgtggtggc ggcattcaac attgtttcca
     8701 cgcttatcat ggtggcgatg gaaaaaacga aagatatagg aattcttcgg gcgttgggcg
     8761 cgacaaagtc ggcggttcgg cggattttta tgcttgaggg catgagctat ggattttggg
     8821 gtatggtcat tggcgtgggt ttgggcgtgt ggtgttctct caagatcaac gacctttccg
     8881 attttct
//
LOCUS       DAOSIO010000052         8773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_7523_length_8773_cov_4.208419, whole genome shotgun sequence.
ACCESSION   DAOSIO010000052 DAOSIO010000000
VERSION     DAOSIO010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8773
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7523_length_8773_cov_4.208419"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..>8773
                     /locus_tag="PK590_03150"
     CDS             44..>8773
                     /locus_tag="PK590_03150"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23627.1"
                     /translation="MTYGVKAIFKGQVAAYANAGFWTRLGGAIGRLAINNFNLSRFAG
                     AAIHMGFGMAFSIKISADVVTIFTGKLDVEEVNRNMADGIMKDIVLMYASIQSQHGLV
                     AMAQNIASPQHWLFVFAFAGLPALVQGFQAGLSKLGSFSSHVLSSMGAGGILSFFRVA
                     PPGAWFSLGIFAGYGQVKMIWDNFFRALFEEAVKEPILGRLFLGWMSEDSQEFAVELL
                     DGLDGHPNRYSIAKLTTGTRHIIQCHTAATKAADVARQKGDTIFAGIYTQIAGFYEQA
                     LTAVKSGNYGHAQQLMIRASNLYNDQTVPATVASEHSALGQEIRAFQQITQRVETTKG
                     VLTQALKDEASPLYKRYGADGLSQMINTLNAYPFMNLGEVSSLFGAAASLRSLAIITG
                     IRLYAEHGETRNTFQTQRDKDILSGFYKAVGVKTANFSAFLRHFGVSTHQAAVISKME
                     SSRLKEFASALAFSADELVPASGEQGLINEHLLANAYASVVLAKGESITPRQAVDLRA
                     MGFSGSKNHETGEIAAFNALSDQGRIEFVGLLRERAATATGFARQSLMISAANETNRR
                     AVAQSLGLEHQVVNFLERTAAKDPGSFKKFMAEFAGAQDLNTLAGRAFFFQTFEGRMF
                     DAAFDFYRGAQDLGNMGRFSLRKTMLFGMRVDVTAFSGLTFDGSLMASAIMGSIGIGE
                     EGYIDSISDFQSRMKMAAVGSVSATPEQFARLGRALGCPARLRDQSEKTQWELLLNEV
                     GKMNAEQYQSFRARVFSKLSDSGGFHEGANFLRLHVDALINTVIELFDEASMGDTLAR
                     IFGAGNNLKFLEWKELDGLENKTPEIRERRFKQVFSEKLEERRKAREASEDEAMALAA
                     AALEAKGASVPSAYKDYVAARKVLATADARLALITALLESPEWDMDRDLAYYKGAFTP
                     EFRSELEELKKELPDKIRQAEETIAAFRKTHPGIRAKLLGEGYQLQLQEAISKDQSVV
                     TAQKTCNDLLAKINNLQAQMSAASVVSSGWETARPLDLYLESEPESELAATKTEMHKA
                     EEALAKAKTDAKSKAENLLARRFMTFGEELRMQTLETSVMRTFVSSAGRARLVQEEID
                     RISDDDSLNSLEKADEISRITGQFSAPGAILAESEVAIQTLIADAMTVRIGNETVLLS
                     SAPATVLDILNTARKDRNQLSANLSELKKKAQSGTLSQGSLKALQKKIAMIERALHRQ
                     QLLCESLSRVYSNMSGLPVNERSTQREINEYQMMVDGIRYEGVLPSDTSQLALTLKDL
                     RSMMDPKNTEVDENLSARLKNLDPDTRQTLIRRGEEANTAQNALRKMADRSSRGTEEG
                     KVHKLSQIGLGDKPSFFALRGRKIGRNDIPQIAGLLEAVRRGGIANASSALNRSQPGA
                     EQSSRQESVLPGAQEIQTPADRAPAVSEALASVTLMSQDNQIVAKTDLLIKLLELMRA
                     DLGLGNLDFAMIDERTLISALNRLFGFADTGSLENLKALAEKLKTSGSGCEELGVRLE
                     KILNDATTKDKYAQAKRDALSLLFGDQLIQSSKSAQTWLARQSKEKQDELAEGGAELQ
                     RQKMISETSPFLARVRDAQSNGKDAIKKSVKLARQAKGNKSRTRSEIAAARRVEARGR
                     IYASSGIVLKTFTWLMMSPRMIPMLRRNGFEDLATAFEEKSYQLHLLEAKLADEVTNE
                     ADSGIKDSALDKGTETQSELVRQLRESVARTTVDQQTADESETVRDARQSLEETERKE
                     GRKKDEAFRNLRSAIGSAEVRENQLAHDKARARLKALQAQKTATASEEAMVASFNALI
                     ATAQAEVDAAKESLEKSIERQKGEQNKFDEESKLTSWRDAPETAHEVARRRALIAHLT
                     ASDMQVDKLFLDSEVINAVREQMNPLVKAWLSFRAFVAGTVAYVNAYLAPSQSTEMDR
                     FIKGKPRFKVWYKPWTLFAKIPSRVTKGMNLAEIRLVLEKFHDAEIDDDVLHDLIDKN
                     GVLTAKGREHQETLRIHLDYILERAGTIETREGKTALRDRMLTETYNAFIEKNTRETL
                     GSSADDWGSLTADQKAQRITQAKNDFEAKNFEEKKSKIVELWMKPQNMEYARKIRILQ
                     QFLEKEEGLGRFGTEIKSALEAEVRAASAVIDVSKVSASAAIESARKSLADERDGLQK
                     KNDVVPLTNAEKERLEEITALIQILNMEDATNVHNLLTQNNTIGSFYEKYVDAISKKS
                     DGKRRNARALVFAKILDQSGAAKDVQTGFKERVKARGDVLTDDVIVDAAAANLMIGTM
                     VRGWYEKQSGVFEEQFMAALLAARGWNGVELATGEGKTLVTAAVAMVNALTGKGSIAV
                     VTKDASAKDAYQEWKSLFDLSGFSAKMRLTNSYDEAEMLASKKTTGRRMLASEIRDAY
                     DADITYVNVQNWAFDFSHDKLQVKDGEGKYIEGGAKNRRLSEFGVTIDEMDSVLIDQA
                     MTSFILSQGAGEVLTGEIRTLRLVFASLVESFSEEVEGYDVDFRSVLPGGKRGQQASR
                     SEADIFVNEDGSWAGVTGTGERKAIKRLMELRDRKEISSDTFKLLVQAQNPLFRGGVR
                     DVTTLGVEDLKLSDLGEQYVNNAVQAKFVYQEGVNYWIDNGEVKLISKELGITQTGQR
                     LNELHQAIEARIRMIQDSDAPEFANHEFKRSGGRKMEIRAESRTESSVTARDVVLSFG
                     KVSGMSGTLNMGQIDSDLAELYGMTCYTIPSHSLNRRINEVVSVATEKDRKLKAASIV
                     KAALQNGQSVLINAHTDRERNEIFEYLRDETEIKDHRIARFGVDGERSEREFVDAIKK
                     DEVMILVATNIAGRGTDFSPGRRMKEAGGLVQINWGMNKRISTDMQAAGRAGRQGAMG
                     ESYTLVVSGVGEQNKEHIYVQGLLRGGHGFFTDRGARNLAIRYDNMLRKQGFSGVSKE
                     GSVSRWLASLGFMGRLKLIWGGRL"
BASE COUNT         2275 a         1984 c         2573 g         1941 t
ORIGIN      
        1 gtgggtcaca tcacttttta gtgagaaggt agcgagacag ggaatgacct atggagtaaa
       61 agcaattttt aaagggcaag ttgcggcgta cgcgaacgcg ggattttgga cgcgtcttgg
      121 cggcgcgatc ggtcggcttg cgattaacaa ttttaatctt tcccggtttg cgggcgcggc
      181 cattcacatg ggttttggta tggccttcag catcaagata tcagctgacg tagtcactat
      241 tttcaccggt aagctggatg ttgaagaagt caaccggaac atggcggacg ggatcatgaa
      301 ggatatcgtg ttgatgtacg cgagcattca gtcccagcat ggacttgtgg cgatggcgca
      361 aaatattgct tcgccccaac actggctttt tgtgtttgct ttcgcgggtt taccggcgct
      421 ggtgcagggg ttccaggcgg gtctttcaaa actgggctcg ttttcatcgc atgtattgag
      481 cagtatgggc gcggggggaa tattgagttt tttccgcgtc gctccgcccg gcgcctggtt
      541 ctcgcttggg atcttcgcgg gttatggtca ggtaaaaatg atctgggata atttcttcag
      601 ggctcttttc gaagaggcgg tcaaagaacc gatcctcggc cgactctttc tcggatggat
      661 gagtgaggac agccaggagt ttgccgtgga actgctcgac gggctggatg gtcacccaaa
      721 ccgctatagc atcgcgaaac tcaccactgg tacccgacac ataatccaat gccacacagc
      781 ggccacgaaa gcggcggatg ttgcacggca aaaaggcgac accatcttcg ccggtatcta
      841 cacacaaata gcgggttttt atgaacaagc gctgaccgcg gtcaagagcg ggaattatgg
      901 ccatgctcag caattaatga tacgggcgtc aaacctttat aatgaccaaa cggtgccggc
      961 aactgtcgcg tcagaacata gcgctctcgg ccaggaaatc cgtgcgtttc agcagataac
     1021 acaaagagtt gaaacgacaa aaggggtttt aacgcaggcg ttaaaggacg aggcgagtcc
     1081 cctttataaa cgctacggag ccgatgggct cagccaaatg atcaataccc tgaatgcgta
     1141 cccattcatg aacctcggtg aagtgtcgtc actatttggt gctgccgcca gcctgaggtc
     1201 cctggcgatc atcaccggta ttcgtctcta tgcggaacac ggagaaactc ggaatacatt
     1261 tcaaacacag cgagataaag atattctttc tggtttttat aaagcggtag gcgttaaaac
     1321 cgccaatttt agcgccttcc tccgccattt cggtgtttcg actcatcagg ccgctgtgat
     1381 ttccaaaatg gaatcaagcc ggctcaaaga gtttgcctcg gcgctggcat tttccgcgga
     1441 tgaactggtg cccgcgtccg gagagcaagg actgattaat gagcacttgt tggcaaacgc
     1501 ttacgcctcc gtcgttttgg ccaaggggga atcgattact ccgaggcagg ctgtggatct
     1561 tagggcgatg ggcttttcag gatcaaaaaa tcatgagacg ggcgaaattg cggcctttaa
     1621 cgctctgagt gatcaaggga ggatcgagtt tgttggcctc ttgcgtgagc gcgcggccac
     1681 ggccacgggt tttgcccggc agtcgcttat gatttctgcc gcgaatgaga cgaatcgtcg
     1741 ggcggtcgcg cagtcgctgg gtctggaaca ccaagtggtc aactttcttg aacgcacggc
     1801 ggcaaaagac cccggaagtt tcaagaagtt tatggcggag tttgcgggcg cgcaggatct
     1861 aaacacgttg gcgggacgcg catttttctt tcagacgttt gaaggacgaa tgtttgacgc
     1921 ggctttcgat ttttatcgtg gggcgcagga cctcgggaat atggggcggt ttagtttacg
     1981 caagaccatg ctttttggga tgcgtgtgga cgtcacggcg ttttccggac ttaccttcga
     2041 tggttccctg atggcgtcgg cgatcatggg atctatcgga atcggagagg agggttacat
     2101 tgactcaatc tctgattttc aaagtcgcat gaaaatggcc gccgttggat cggtgagcgc
     2161 gacgcctgaa cagttcgccc ggctggggcg agcgctaggt tgtcccgccc gcctgaggga
     2221 tcaatccgaa aagacgcaat gggagttact cctgaacgag gtgggcaaga tgaacgcgga
     2281 gcagtatcag tcgttcaggg cacgggtttt ttcgaaactg tccgattccg gcggatttca
     2341 tgaaggtgct aacttcttga gattgcatgt tgatgccctt attaacacgg taattgaatt
     2401 gttcgatgaa gcctcaatgg gggacacgct cgcgcggatt ttcggcgctg gaaacaatct
     2461 gaaatttttg gaatggaaag aactcgatgg tttagaaaat aagacgcccg aaattcggga
     2521 aagaaggttc aaacaagttt tttccgaaaa gttggaggag agacggaaag cccgtgaggc
     2581 gagtgaggac gaagcgatgg ctcttgccgc ggccgcgctt gaggccaaag gggcatctgt
     2641 gccttcggct tataaggatt acgtggccgc acgaaaggta ttggcaactg ctgacgcgcg
     2701 gttggcgctg ataacagctt tgctggaatc gcctgaatgg gatatggaca gggatctagc
     2761 ttattataag ggagctttca cgcccgagtt tcgtagtgag cttgaggagt tgaagaaaga
     2821 acttcctgac aaaattcgcc aggctgaaga aacaatcgcg gcatttcgga aaacgcaccc
     2881 gggaatcaga gcgaaacttc tcggagaagg ctaccagcta cagctacagg aagcgatatc
     2941 gaaagatcaa tcagtggtta ctgcccaaaa gacctgtaac gatcttctgg caaaaatcaa
     3001 taatctccaa gcacagatga gcgccgcatc agtcgtctct tcaggatggg aaacggcgcg
     3061 tcctcttgat ctgtatcttg aatcagaacc ggaatcagaa ctggcggcaa ccaaaacgga
     3121 aatgcacaag gccgaagaag cgctggcgaa ggccaaaact gatgctaaat cgaaagcaga
     3181 gaatcttctc gcccgacggt ttatgacatt cggcgaggag ctccgaatgc agacactcga
     3241 gacatcagtg atgcggacat ttgtatcgtc cgccggacgc gcccgcctgg ttcaggagga
     3301 gatcgatagg attagtgatg acgatagtct gaatagcctg gagaaggccg atgaaatatc
     3361 tcgaatcacc ggacaattta gtgctcccgg ggcgatcctc gcggaatcgg aagtagcgat
     3421 tcaaacgctg attgcggatg cgatgactgt gagaatcgga aatgaaaccg tcctgctttc
     3481 aagcgcgccg gcgaccgtac tggacatcct caacacggct cgtaaggatc gtaatcagtt
     3541 atcggccaat ctttctgagc tgaagaaaaa agcccagtcc ggaactctca gtcagggttc
     3601 cctcaaggcg ctccagaaaa agatagcgat gatagagcgt gcccttcacc ggcaacagct
     3661 tctctgcgag tcactcagcc gtgtttacag caacatgagc ggccttcccg tgaacgagag
     3721 atcaacgcag agggaaatca acgaatacca aatgatggtt gatgggatcc gatatgaagg
     3781 tgttttgcca agcgatacgt ctcaactggc attaactctc aaagatttgc gaagtatgat
     3841 ggatcctaaa aatactgagg ttgacgaaaa tttgtcggca cgtcttaaaa atcttgatcc
     3901 agatacgcgg cagaccctca tccggcgcgg tgaggaggca aataccgccc aaaatgcgct
     3961 tcgaaaaatg gcggatcgtt cctcgcgcgg aaccgaagag ggaaaggtcc acaaactttc
     4021 ccagatcggg cttggtgata aaccttcgtt ttttgccctt cgcggccgca agattggcag
     4081 gaacgatatt cctcagattg ccggtcttct tgaagccgtc aggcgtgggg gaatcgccaa
     4141 cgcctcaagt gcattaaata gatcgcaacc tggcgccgaa cagtcttctc gccaggagag
     4201 tgttttgccg ggtgctcaag aaatccagac gccggcggac cgtgcgcccg cggtttccga
     4261 ggcattggcc tcagttacct taatgtctca ggataatcaa atagtcgcga agacggatct
     4321 gttgatcaaa ttgctcgaac tgatgcgtgc agacttgggg ctcgggaatt tggattttgc
     4381 catgatcgat gaacgcacac tgattagcgc cttgaaccgc ctcttcggtt ttgcggacac
     4441 gggaagtctt gagaatctga aggcgctggc cgagaagctc aagacgagcg gttcagggtg
     4501 tgaagagttg ggagtgcggc ttgagaagat cctgaacgac gctaccacaa aagacaagta
     4561 tgctcaggca aaacgagacg cgttgtcgct tctctttggt gatcagttga ttcagtcatc
     4621 aaaatcggcg cagacatggc ttgcgcggca atcgaaggaa aaacaggatg agctcgcaga
     4681 aggtggcgcc gaactccaga gacagaaaat gatttcggaa acatcgccct tccttgcgcg
     4741 cgtccgagac gcgcaatcaa acggcaagga cgcgatcaag aaaagcgtta agttggctcg
     4801 ccaggccaaa ggaaacaaga gtcgaacacg ttccgaaatt gccgccgctc gtcgcgtcga
     4861 ggcaagggga cgaatttacg cgtcttccgg catagtcctc aaaacattta cgtggctgat
     4921 gatgtcacct cgaatgatac cgatgttacg gcgaaacgga tttgaggacc ttgcgacggc
     4981 atttgaggaa aagtcatatc agctccacct gcttgaagca aaacttgcgg atgaggttac
     5041 taacgaggct gattctggga taaaggattc agctcttgac aaggggacag agacacagag
     5101 cgaacttgtc cgtcagctta gggagagtgt ggcgagaacg actgttgatc aacagactgc
     5161 tgacgagtct gaaacagtgc gcgacgcgcg tcaatcgctt gaggaaacgg aacgcaaaga
     5221 ggggcgtaag aaagatgaag cgttccgtaa tctccgctca gcgattggca gtgccgaggt
     5281 tcgcgagaat cagttggcgc atgacaaggc ccgggcccgc ctgaaagcac ttcaagccca
     5341 gaaaactgcc acagcgagtg aggaagcgat ggttgctagt ttcaacgcgc taatcgcaac
     5401 tgctcaagcg gaagtagatg cggcaaaaga atctctcgaa aaatccatcg agcgccaaaa
     5461 gggtgagcaa aataagtttg atgaggaatc aaagcttact tcttggcggg acgctccaga
     5521 aacggcacat gaagtagcgc gccggagagc gctaattgcg catttgacgg cctcagatat
     5581 gcaagtggat aagttgttcc tggattcgga agtgatcaat gccgtccggg aacagatgaa
     5641 tccgttggtc aaggcatggc tgagtttccg tgcgttcgtg gcgggaacgg tcgcttatgt
     5701 gaacgcctat ttggcgcctt cccaaagtac agagatggat cgtttcataa aaggaaaacc
     5761 gcggttcaag gtgtggtata aaccgtggac tttgtttgcc aagattccgt cacgtgtcac
     5821 caaaggcatg aatcttgcgg aaatacggct ggttctggaa aaatttcacg acgcggaaat
     5881 cgatgatgat gtcttgcatg atctgattga caaaaatggc gtgttgaccg cgaaaggaag
     5941 agaacaccag gagacgctca ggattcatct ggactacatt ctcgaaaggg cagggacaat
     6001 cgagacgcga gaaggcaaaa ccgctctcag ggatcggatg cttaccgaaa catacaacgc
     6061 ttttattgag aaaaacacgc gcgaaacact gggtagcagc gcggatgact gggggagttt
     6121 aacggcggat cagaaagcac aaagaatcac gcaggccaaa aatgactttg aagcaaaaaa
     6181 ttttgaagag aaaaaatcga agattgtaga gctatggatg aaaccccaaa acatggaata
     6241 cgcccgtaaa atacgtattc tccagcagtt cctcgagaaa gaggaagggc tcggccggtt
     6301 cgggaccgag atcaaatccg cgctcgaagc ggaggttcgc gccgcgagtg ccgtgattga
     6361 tgtttcgaag gttagcgcga gtgccgccat tgaatcagcc agaaaaagtc ttgcagatga
     6421 acgagatggg cttcagaaaa aaaacgatgt tgtgcctttg actaacgcgg agaaggagcg
     6481 gcttgaggaa attacggcat tgattcaaat ccttaatatg gaagatgcta cgaatgttca
     6541 caacctgctc actcaaaaca atacgatcgg cagtttttat gaaaagtatg tggatgcgat
     6601 cagtaagaag tctgatggaa aacgccggaa tgcacgagct cttgtctttg ccaaaattct
     6661 cgatcaatcg ggagccgcga aagatgttca gaccgggttt aaagagcggg tcaaagcccg
     6721 tggcgacgtg ttgacggatg atgtgatcgt ggatgccgcg gccgctaatt tgatgatagg
     6781 aacgatggtt cgcggatggt atgaaaaaca gtcgggtgtc tttgaagagc agtttatggc
     6841 cgcgcttctg gcggcgcgcg gctggaacgg cgtggagctt gcgacgggtg aagggaaaac
     6901 gcttgtgacc gccgctgtgg ccatggtcaa cgctctcacg ggcaagggtt ctattgcggt
     6961 cgtcaccaag gatgcctcgg cgaaggatgc gtaccaggag tggaaatccc tttttgacct
     7021 ttcgggattt agcgcaaaga tgaggctgac gaattcttat gatgaggccg aaatgctcgc
     7081 ttccaaaaaa acaaccggca ggcggatgct cgcttccgag attcgcgacg cttatgacgc
     7141 ggacatcact tatgtcaatg ttcagaactg ggcgtttgat ttctcccatg acaagctcca
     7201 ggtgaaggac ggggagggta aatatatcga aggcggagcg aaaaaccgtc gattgtccga
     7261 gtttggagtc acgatcgacg agatggacag cgtgctaatc gaccaggcca tgacctcctt
     7321 tatcctttcg cagggggccg gtgaggttct gaccggggaa atccgaactc tccgactcgt
     7381 tttcgcgagc cttgtcgaga gtttttcgga ggaagtggag ggatatgatg ttgatttccg
     7441 ttctgtgctc cccggcggaa aacgcggcca gcaggcaagc cgttccgagg cggatatttt
     7501 tgtgaatgag gacggttcgt gggcgggtgt tacaggcacg ggagagcgaa aagcgattaa
     7561 acgactgatg gaattgcgag atcggaaaga aatttcgtcg gatactttta aactgcttgt
     7621 gcaggcccaa aacccgcttt tccgcggggg agtccgggat gtgactacac tgggagtcga
     7681 agatctgaaa ctttcggatc tcggcgaaca gtacgtcaat aacgcggtgc aggcgaaatt
     7741 tgtttatcag gaaggggtta attactggat tgataacggg gaagtgaagc ttatttctaa
     7801 agaactcgga atcacgcaga cggggcaacg cttgaatgaa cttcaccagg cgattgaggc
     7861 gcgtattcgg atgatccagg acagcgatgc tcctgaattc gcgaatcatg aatttaagcg
     7921 tagcggcgga aggaaaatgg aaatacgcgc tgaaagccga acggaatcga gcgtaaccgc
     7981 gcgcgacgtg gtgctttcgt tcggcaaggt atcggggatg tccgggacgc tcaatatggg
     8041 tcagattgat tccgatcttg ccgaactcta cggcatgacc tgctatacga tcccgagcca
     8101 ttcgttgaac cgcaggatca atgaagtggt gtcggtggcg acggaaaaag accggaaact
     8161 taaggccgcc agcatcgtca aggcggcgct ccagaacggg cagtctgttt tgatcaacgc
     8221 tcatacggat cgtgaacgta acgaaatttt tgaatatctc agggacgaga cggaaattaa
     8281 ggaccatcga atagcccggt ttggtgtaga cggcgagcgt agcgagcgcg agtttgtgga
     8341 tgcgatcaaa aaagatgagg ttatgattct tgtggcgact aatatcgcgg gtcgcgggac
     8401 tgatttttcg cctggacgca ggatgaagga ggcaggtgga cttgtgcaga tcaactgggg
     8461 catgaacaag agaatcagca cggacatgca ggccgcggga cgcgcggggc ggcagggcgc
     8521 gatgggtgaa tcctatacct tggtggtttc cggtgtggga gaacagaata aagaacacat
     8581 ctacgtccaa gggcttttgc gcgggggaca cggtttcttt acagatcgcg gagcaagaaa
     8641 tctggcgata cgctatgaca atatgctccg caaacagggt ttttcgggag tttccaagga
     8701 aggttcagtt tcccgttggc tcgccagcct cggttttatg ggacgtctta aattaatttg
     8761 gggcggaaga ttg
//
LOCUS       DAOSIO010000053         8544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_7741_length_8544_cov_5.162210, whole genome shotgun sequence.
ACCESSION   DAOSIO010000053 DAOSIO010000000
VERSION     DAOSIO010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 8544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8544
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7741_length_8544_cov_5.162210"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1259)
                     /locus_tag="PK590_03155"
     CDS             complement(75..1259)
                     /locus_tag="PK590_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23628.1"
                     /translation="MSRLKEIEGRGKSASGSSAGFYRYGRTILFVFVFGAALAGLAAN
                     FDGERSGAESVEQRQLNELKRYYSAGARSGVAGVPQIPMAISFYNDAVQFFEKGEYEI
                     AREAATESIHLDPRNPLAWELLGEIENLRQNFDKAGEYYKKSYGLNASPRVKAKLEKL
                     NKERLVEEKMDTYHEEHFVIKYDRSDSQYEGFFLKNLLREIYRKVSRDLGFYIRHKIV
                     VIFYKPEDFRYVTEQPHFVGGIYDGKIRLPAYRKGFSERELRATAAHEMTHAFVAYLS
                     SLRAPAWIQEGLAQVEENSVRPIDLSVLQAAFRSQKLYPVEEMLLMELKPEMPHEQVS
                     VFYQQSFSFVSYLVKRFGMYRMKEILQEMRKGSDSFAAIEEVLKISPHRLEKEWLTTI
                     RK"
     gene            complement(1256..2377)
                     /locus_tag="PK590_03160"
     CDS             complement(1256..2377)
                     /locus_tag="PK590_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOG23629.1"
                     /translation="MERHIKARQYQRIRRRLTLIHLVLTPALLVVFLLSGLHLLFKDW
                     AMAIAGSGDWLVIACYFTLFSLFFLVFDLPFSYYAGYRLEHQFALSNQTLGAWAGDFM
                     KKSLLSFALSLALVELLYLLIRVAPETWWIWAWGAYAFVSYFLGKIFPVLIVPLFYKY
                     GNVEDQALAERIKGLASRYGMPLQNVYSLNLSKTTKKANAAFMGIGKTRRVVLSDTLI
                     ANFSHDEIETVVAHELGHFRARDIWRLLGFGLIISAAAFYAGFHGMNLWARSFGIQSA
                     SDVAGLALLLLIFYGVSFALMPAQNAYSRRREFAADRFALNACRTPDVFISCMKKLGT
                     VNLAEENPPAWYEWMFYDHPSLARRIRVGEQYKIKKGGT"
     gene            complement(2505..3317)
                     /locus_tag="PK590_03165"
     CDS             complement(2505..3317)
                     /locus_tag="PK590_03165"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOG23630.1"
                     /translation="MKILTLNTWQERGPWRERWDLIFRGLKEYAPDIAGFQEVFNMEW
                     AESIRERSGYPWLCVSGEHSGLIFLSKYPPVGQECLIMKTRSPLEEYRRYAFGAMFET
                     PRGKVPCFNTHLSWQVSDEPARAGQVRELDEWVREKAGLSPAVVMGDFNTAPGTAPIH
                     FLTSRGWSDTFEQANPWVRDFELSGGARQSPDGFSNSQRKGKAYLTWDYKNPYAEKER
                     DKMAERRIDYIFVTRRDGPFSRIRSSRVVFDFPEGEVWPSDHFGVLTEFSNG"
     gene            complement(3417..4202)
                     /locus_tag="PK590_03170"
     CDS             complement(3417..4202)
                     /locus_tag="PK590_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG23631.1"
                     /translation="MKVYKEKKLGAVAGLFYSLVLWLGTVTLTVFFTLVDIFLALPVS
                     LVLDRDARRLPHKVTNLWARGIIAWNPIWRLTVTGQSNIRKGKNYVIVANHQSLLDIL
                     AVAAALPLNFKFLAKKELFSIPFLGWAMSCAGYIPVDRSSHESGRRAMKRIQSLLAKK
                     LSVLLFPEGTRSPDGKIHAFKTGAFKLARENKTEILPVVIDGTGRALPKKSLILRNRS
                     RFVVSIGEPVCLENLGDQSLEEARDKIRHEMAGRLERLRRNRR"
     gene            complement(4375..>8544)
                     /locus_tag="PK590_03175"
     CDS             complement(4375..>8544)
                     /locus_tag="PK590_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23632.1"
                     /translation="VFFSFTQVVSAVPGMGVEIAVQGETPGFIHLSIPADLASLDDIY
                     EAPPSPSPRLILHVQNAHANYDAQTKIKNLLEYLSAQYAVRTIFVEGASGTLDPDYLK
                     FFPDQARNRELADRLARQGELTGAELFILEADRQNIRAEGIESTDLYRKNYEALKKVY
                     ENETVVLQFIEGLESRLRTLASQVAGRDLLRMISEWKRFERGQREFMPYVRSLAEDAR
                     QFLRLDLESLFSQVEWPQLTRLLTIQDMEKELQPSRAVLEQEKLLALLRRSEASPELI
                     DGIANFRDQHISLARTGSRGSPQTLQPRYLLERLVNEMGPQGFKFSDYPAFSLQAGYL
                     ILKSELDSRALFDEIHRLFQMILDKMAVSEAQKKLLALYRDEELVRKLLRLELTRRDW
                     RELQSRRDELAPDPLMERLKSLAEDARVNLKLGSTGWDSKKGSPKLRSRILELYGAAD
                     LFYDYARARESAFFEKISEVMIAGRETSSVLITGGFHTDGMSDIFRENQISYGILTPR
                     LSEKSDESLYRNSMLQRGPGIFDTSTIELFSKMLPPDVLKSMGANPARVFGQNLGLSL
                     ELTAETSDTMDDIEAQLQYLQTFLPRFDLSLNYEVDDDGFRVLLLKNGAPLKNDRGEV
                     FLVSIEKNPALAGKFRISRGKKNWVPADKLGQEILRFSVRDHRTGLTFQTGTLDPLSQ
                     PADLGRSEVRGKWQAFRSWAGEHRRIFLVGGMTVVMWVGMFVAHQSIVYARNSAEPMY
                     SSKNFTFEVMSREEAERNKCLIVTNANMFRADTGGPAGLLLRDGVVVNPFNPNTRGSA
                     QARVVVLFHPLYLSRIVTTEEYAANREFYEKPGNGWCGFQIGPMADLGRSPERDEAIK
                     KWFEGNPGRRVIVGFDKEGNLYVKEFGSDVFGMGNMSWKKFHSRVLRWFKKYGIVKAG
                     FADGGNTGYKQLGLKKPYYVITAHPRSEVRISPDVSDVSPAIGIPTQKEPAIPTLPAV
                     GPGPVEGRPSGLVDVDGIQKALDGAVGDGKVTVKKDGTDGIAITTPGKGVVVVRGFVE
                     LSVQGKQIRITHRVSSTGKTRSDLLPIELPFKIDRPMEVDAFLAMRSVEQMINTVTPE
                     IRETLEKISFRLLRNLGIELDQTSPALTVTVALTWGIVNMKKEPESAFLLLREIARLV
                     DTRTLTGLRQRLSEQLPGEAIPFSGEAGNVVKMIDASVLSTRESTREVLEQLMFTYLF
                     NPEVRLNILALGDEIREPQRENGIWLAETFNRWAATWTGVERVARPVAVGVFNRSKAA
                     REAQGFLNNVKAQGRYVVAGDESLLMGGLPDGLLSNMQALAPVLFESGVNTPELQPMA
                     FLAAARASQRIGTAKGESVSVLVDMLRDPLPDDGYQYENGRFVINSHTLSAIKNLQTL
                     FAAFQKLQASA"
BASE COUNT         2078 a         2548 c         2018 g         1900 t
ORIGIN      
        1 ggaatcccgg aaggagcgaa gttccgtgag cgccacttcg ggcaagacct gaccgttgcc
       61 cgcgcgcgac ttttttattt tcggatcgtc gtgagccatt ccttctccag tcggtgggga
      121 gaaattttca ggacttcctc gattgccgcg aaggaatcgc tccccttgcg catttcctga
      181 aggatctctt tcatccggta cattccaaac cgctttacca ggtaactgac aaaactgaaa
      241 gactgctgat aaaaaacgct cacttgctca tgcggcattt cgggtttcaa ctccatcaga
      301 agcatttcct cgaccggata aagtttttgg gagcgaaacg ccgcttgaag gaccgagaga
      361 tcaatcggac ggacgctgtt ttcctccact tgggccaacc cttcctggat ccacgccgga
      421 gcccgcagac tgctcaggta agccacaaac gcgtgggtca tttcatgagc ggcggtcgcg
      481 cgaagttcac gctcgctaaa tcccttccgg taagccggca gtcttatttt cccgtcgtaa
      541 atgcctccga caaaatgcgg ctgttcggtc acgtaccgga aatcctccgg cttgtaaaaa
      601 atcaccacga tcttgtggcg aatataaaac cccagatccc gcgacacttt ccggtaaatc
      661 tcccggagaa gattcttgag aaaaaatccc tcgtattgtg aatccgatcg gtcatacttg
      721 atcacgaagt gttcctcgtg gtaggtgtcc atcttctctt ccacaaggcg ttccttgttc
      781 aatttttcca actttgcctt gacgcgcggg gacgcgttca gcccgtaact ttttttgtaa
      841 tactcgccgg ccttgtcaaa attctggcgc aaattttcaa tttcacccaa aagctcccag
      901 gccagcggat ttcgaggatc cagatgaatg gattctgtcg ccgcttcccg ggcgatctcg
      961 tattccccct tttcgaagaa ttgcaccgcg tcattgtaaa agctgatggc catgggaatc
     1021 tggggcactc ccgcgacacc cgatctcgcc ccggccgaat aataccgttt cagttcattc
     1081 aactgacgct gttccaccga ttccgctccc gaccgttcac cgtcaaaatt cgccgcgagc
     1141 cccgccaagg ccgccccgaa aacgaacaca aaaagaatcg tccggccgta acgataaaat
     1201 ccggccgaag atccggaagc agacttgccc cggccctcga tttccttcaa ccggctcatg
     1261 tcccgccctt tttgatcttg tattgttctc ccactcgaat tctcctagca agcgacggat
     1321 gatcgtaaaa catccattca taccacgcgg gcggattttc ctccgccaga ttcactgtcc
     1381 cgagtttttt catgcacgaa ataaaaacat ccggagtccg gcaggcgttg agggcgaacc
     1441 ggtcggccgc gaactcccgc cgtctcgaat aagcgttctg cgccggcatt agcgcaaagc
     1501 tgactccgta gaagatcaga agaagtaagg caagccccgc gacgtccgag gccgactgaa
     1561 ttccgaacga ccgggcccac aggttcattc cgtggaaacc ggcgtaaaaa gcggcggcgg
     1621 aaattatcaa gccaaaaccc aagagccgcc agatgtcccg cgccctgaaa tggcccagtt
     1681 catgcgcgac aaccgtctcg atttcatcgt gcgaaaaatt cgcgatcagc gtgtcgctca
     1741 acaccacgcg ccttgtcttt ccgattccca tgaacgcggc gttggccttt tttgtcgtct
     1801 tgctgaggtt cagggaataa acgttctgaa ggggcatgcc ataccgcgaa gcaagtccct
     1861 tgatccgttc cgccagcgcc tggtcctcca cgttcccgta tttgtaaaaa agaggaacga
     1921 tcaggaccgg aaaaattttt ccgagaaagt agctcacaaa cgcgtacgcg ccccaggccc
     1981 agatccacca ggtctcggga gcaacccgga tcaggagata aagcaattcg accagcgcga
     2041 gcgacagcgc gaaagaaagc agtgattttt tcataaaatc tcccgcccag gcgcccaggg
     2101 tctggttgga caaggcaaat tgatgttcaa gccggtatcc cgcgtaatac gaaaacggaa
     2161 gatcaaagac cagaaaaaaa agagagaaca aggtaaagta acacgcgatc accagccagt
     2221 cgccggaacc ggctatggcc atggcccagt ctttaaagag aagatggaga ccgctcagga
     2281 ggaacacaac aagtaaagcg ggcgtcaaaa ccagatggat gagagtaagc ctgcggcgaa
     2341 ttcgctgata ttgtcgcgcc tttatatgac gttccatgtc tgaattttgt ctccctttat
     2401 gaaaaaaata ccaaaataaa aactccgaaa aacgcgcgtc ttccccgact tcttcccgga
     2461 aaaaacaatc ccgggattat cgttggctcc cgccccgcaa acactcaacc gtttgaaaac
     2521 tccgtaagaa ctccaaaatg gtcggacggc caaacctccc cctcgggaaa atcaaaaaca
     2581 acccgcgatg agcgaattcg cgaaaaaggc ccgtcccgcc gggtgacaaa aatataatcg
     2641 atgcgccgct cggccatttt gtcccgttcc ttttcggcgt acggattttt gtagtcccac
     2701 gttaaatagg ccttcccctt tctttgagaa ttcgaaaacc cgtccggaga ttggcgcgcg
     2761 cccccgctta attcgaaatc cctcacccaa ggattggcct gttcaaaagt gtcgctccat
     2821 ccccttgacg tcagaaaatg aatcggggca gtccccggag ccgtattaaa atcccccatc
     2881 accaccgccg gcgacaaacc ggccttttcc cgtacccatt cgtccagctc cctcacctgc
     2941 cccgcgcggg ccggctcatc agaaacctgc cacgaaagat gcgtgttgaa acacgggacc
     3001 ttgccgcgag gcgtctcaaa catcgcgccg aacgcgtaac gacggtactc ctcaagagga
     3061 gatcgcgttt tcatgatcag acattcctgc ccgactggcg gatacttcga aagaaagatt
     3121 aagccagagt gctccccgct cacgcaaagc cacgggtatc cggatcgctc ccggatcgat
     3181 tccgcccact ccatgttaaa aacctcctga aatccggcaa tgtccggagc gtattcttta
     3241 agcccccgga aaatcaggtc ccaccgttca cgccacgggc cgcgttcctg ccatgtgttt
     3301 aaagtcaata ttttcatttt tatttactct cgtgacgacc tcggaccccg tcaaaaattc
     3361 cgggacggga aacaagacat tggtggaagc tccccggatc atcgcctccg gaataactag
     3421 cgacggttcc ggcgtaaccg ttcaagtcgt ccggccatct cgtgccggat cttgtcccgg
     3481 gcttcttcga gcgactgatc gcccagattc tccagacaga caggttcgcc gatcgaaacg
     3541 acaaacctgc tacgattacg aaggatcaga ctttttttgg gaagcgcgcg accagtcccg
     3601 tcgatcacca ccggaagaat ctccgtttta ttttcccggg cgagcttgaa ggcccccgtc
     3661 ttgaacgcgt gaatttttcc gtccggactc cgggtccctt cggggaacaa aaggacgctc
     3721 agttttttcg ccagaagcga ctgaattctt ttcatcgccc gtcggccgct ctcgtgactg
     3781 ctccggtcca cggggatgta tccagcgcag gacatcgccc atccgagaaa cgggatcgag
     3841 aaaagctcct ttttggcaag aaacttaaag ttcagcggaa gcgccgcggc aacggcaaga
     3901 atatcaagca ggctttggtg attggccaca atcacgtaat tttttccctt ccggatattg
     3961 gactgtcccg tcaccgtcag ccgccagatg gggttccacg cgataattcc ccgcgcccag
     4021 agattcgtca ccttgtgcgg cagacggcgc gcgtcccggt ccaggacaag agagaccggt
     4081 agggccagaa aaatatccac cagcgtaaaa aaaacggtca acgtcaccgt tcccagccac
     4141 agaaccaggg aataaaaaag accggccacg gcgcccagtt ttttttcctt ataaaccttc
     4201 atactttgag tccttcctca aatggcgtct gccacaagac gaagatggat tttagcggca
     4261 ccaggctcat ttttaaacct ctttttcccg tcacccgctc ccaaaggtcc aaactccggg
     4321 caaaccggtt tttgggggaa aattcggacg gaaggaaaca atcccggaaa gactttacgc
     4381 ggaagcctgg agcttttgaa atgccgcaaa caatgtctgg agattcttga tcgcgctcag
     4441 ggtatgacta ttgatgacaa atcgtccgtt ctcgtattga tacccgtcgt caggtagcgg
     4501 gtcccgaagc atgtcaacca gcacgctgac actttctcct ttggccgttc cgatacgctg
     4561 ggaagcccgt gccgccgcca ggaacgccat cggttgcaac tcaggtgtgt tgactccgga
     4621 ttcaaacaaa accggagcca aggcctgcat gttcgaaaga aggccatcgg gaagaccccc
     4681 cataagaagc gactcatctc cggcaaccac gtaacgtccc tgggccttta cattatttaa
     4741 aaatccctgt gcttccctag cggccttgct tcgattgaaa acccccacag ccaccggcct
     4801 cgcgaccctt tcaacgccgg tccatgtcgc ggcccaccgg ttaaaagttt cagccagcca
     4861 gattccgttt tccctttgtg gctccctgat ttcatccccc aacgcgagaa tattcagacg
     4921 aacttcggga ttaaagagat acgtaaacat aagttgctca agaacttcac gggtactttc
     4981 acgcgtcgaa aggaccgacg cgtcaatcat cttgacgaca ttccccgcct ctccgctgaa
     5041 cgggatcgcc tcacccggca gttgttcact aagacgctga cgcagtccgg tcagtgttct
     5101 cgtatcaaca agtcgcgcga tttctctcag gagcagaaaa gcggactccg gttctttttt
     5161 catgttcacg atgccccagg tcagcgcaac agtcaccgtc agagcgggcg atgtctggtc
     5221 cagttcaatc cccagattcc ggagtaaacg gaatgaaatc ttttccagtg tttcccggat
     5281 ctccggagta accgtattta tcatttgttc caccgaccgc atcgccaaaa acgcatccac
     5341 ctccatcggg cgatcaatct tgaacggcag ctcaatcggc aaaaggtcgc tccgtgtctt
     5401 tccggtcgat gacacccggt gcgtaatccg gatttgcttc ccctggaccg aaagttccac
     5461 aaatccccga accacgacaa cacccttccc gggagtcgtg attgcgattc catcggtccc
     5521 gtcttttttc accgtcactt ttccgtcacc caccgctccg tcaagcgcct tttgaatgcc
     5581 atcgacatcc accaaccccg acggacgccc ctcaaccggc cccggcccaa ccgcgggcag
     5641 cgtgggaatt gcgggctctt tctgggtcgg tatcccaatc gcgggactta catccgaaac
     5701 atctggactt attctcactt ctgagcgagg atgagcagtt atgacataat acggtttttt
     5761 taacccgagt tgcttataac cagtattccc accatcagca aaacccgctt tcacaatacc
     5821 atatttctta aaccaacgaa gaacacgcga atgaaatttt ttccaagaca tgtttcccat
     5881 tccaaacacg tcactaccaa attccttcac atacaaattt ccctccttat caaaaccaac
     5941 aataactctg cgcccaggat taccttcaaa ccacttcttg atcgcctcat cacgctcggg
     6001 gctcctcccc aaatccgcca tgggcccgat ttggaacccg caccacccgt tccctggttt
     6061 ttcgtaaaac tcacggtttg ccgcatactc ctcggtagta acaattctgg agaggtaaag
     6121 aggatgaaac agaaccacca ctctcgcttg cgcgctccca cgggtattag ggttaaaggg
     6181 atttaccaca acaccgtccc tcagcaaaag gcccgcggga ccccctgtat cggcacggaa
     6241 catatttgcg tttgtaacga tcaagcattt atttctttct gcctcttcac gagacataac
     6301 ttcaaacgta aaattcttcg aactgtacat cggttcagct gaatttctcg cgtagacaat
     6361 ggattgatgg gctacgaaca tccccaccca catcacaact gtcatcccgc cgacaagaaa
     6421 aatccttctg tgttctccgg cccatgaacg aaatgcctgc catttccccc ggacttcaga
     6481 acgccctaag tcggcaggct gagacagcgg gtccagagtt cccgtctgaa atgttagtcc
     6541 ggtccggtga tccctcacgg aaaaccgcaa aatctcctgg ccaagtttgt cggcaggaac
     6601 ccagtttttc ttccctcgcg agatccgaaa ttttccggcc agggcgggat ttttttcgat
     6661 gctcaccaaa aaaacttccc ctcggtcatt cttcagggga gcgccatttt taagcaagag
     6721 caccctgaag ccgtcatcgt cgacttcata attcaaactt agatcaaacc ggggcagaaa
     6781 cgtctgaaga tattgaagtt gagcctcaat atcatccatc gtgtccgaag tctcggccgt
     6841 aagctccagg gacaggccca aattctggcc aaacacgcgc gcggggttgg cgcccatgga
     6901 cttcagaaca tcagggggca acatcttgga aaacaattcg atcgtggacg tgtcaaaaat
     6961 gccgggcccc cgttggagca tcgagttccg ataaagcgat tcgtcggatt tttcagaaag
     7021 acgcggcgta aggatcccgt agctgatctg gttttccctg aaaatgtccg acatcccgtc
     7081 cgtgtgaaat ccccccgtga tcaggacgga actcgtctca cgcccggcga tcatcacttc
     7141 ggaaattttt tcaaaaaaag cggattcccg ggcgcgggcg taatcgtaga acaagtcagc
     7201 ggcaccgtag agctccagaa ttcgcgaccg gagcttcggg cttccttttt tcgaatccca
     7261 cccggtggaa ccaagtttca ggttcacccg ggcatcctcc gccagtgatt tcagacgctc
     7321 catcaacgga tctggcgcca gttcgtcccg ccgtgactgg agctcccgcc agtcacgccg
     7381 ggtcaattcc aggcggagta atttgcgaac cagctcttcg tcacggtaaa gcgcgaggag
     7441 ttttttctgc gcttcgctca cggccatctt gtctagaatc atttgaaaca ggcgatgaat
     7501 ttcatcgaaa agcgcccgtg aatcgagttc gcttttgagg atcaaataac ccgcctgtag
     7561 gctgaaagcg gggtaatcac tgaatttaaa accctggggc cccatttcat tcaccaaccg
     7621 ctcgagaagg tagcggggtt gaagggtctg cggagaaccc cgcgaaccgg tccgcgccag
     7681 actgatgtgc tgatccctga agttcgcgat cccgtcgatc aattccggtg atgcttctga
     7741 acgccgcaat agcgccagca gtttctcctg ttcaaggacc gcccgggagg gttgaagctc
     7801 cttttccata tcctggatcg tgagaagccg cgtcagttgg ggccattcca cctgcgaaaa
     7861 aagactttcc aggtccagtc gcaggaattg ccgggcatcc tccgcgagcg aacggacata
     7921 aggcataaat tcacgttgtc cccgctcaaa tcttttccat tccgaaatca tccggagcag
     7981 gtcccggccc gcgacctgac tcgcgagggt ccggagacgc gactcaagtc cctcgataaa
     8041 ctgaagaacg accgtctcgt tctcgtacac cttcttgaga gcttcataat tcttgcggta
     8101 gagatccgtc gactcaatcc cttccgcacg gatattttgc cggtccgcct ccagaataaa
     8161 cagttccgct ccggtcagct cgccctggcg agcaagacgg tcggcaagct cgcgattacg
     8221 cgcctggtcc gggaaaaatt tcaaatagtc gggatcaagc gtgccggaag ctccttcgac
     8281 aaaaatagtg cgtaccgcgt actgggcgct cagatactcc agaaggttct tgatcttggt
     8341 ctgggcatcg taattggcgt gagcattctg gacgtgcagg atcagccggg gcgaaggaga
     8401 cggaggcgct tcataaatat catcaagact tgccagatcc gcgggaatac tcaaatgaat
     8461 gaaacccggg gtctcgcctt gcaccgcgat ctcgaccccc attcccggaa ccgcggagac
     8521 aacctgggtg aatgaaaaaa atac
//
LOCUS       DAOSIO010000054         8457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_7831_length_8457_cov_4.533682, whole genome shotgun sequence.
ACCESSION   DAOSIO010000054 DAOSIO010000000
VERSION     DAOSIO010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 8457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8457
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7831_length_8457_cov_4.533682"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PK590_03180"
     CDS             complement(<1..448)
                     /locus_tag="PK590_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23633.1"
                     /translation="MVKKITVSVSLFVILAAVSVLVAILVQRVTGRWGFAGAIVNSIL
                     SVGVVLSLYVPVRKFLGYVMDRYLFSDEGDYQKLLKDASRGMSSVESLNHLAGLILHF
                     ITMKMRVKNAAILIRGVRGPDFYLVCQRGYDKQFLSLELSAEDPLLQ"
     gene            complement(626..2812)
                     /locus_tag="PK590_03185"
     CDS             complement(626..2812)
                     /locus_tag="PK590_03185"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG23634.1"
                     /translation="MMNSIFNPQNYYLNGYAMPHFIVGVLISFEGLFVFFQGKKTITH
                     IAYTVTTVTAGIWLTGVGVACSMRDEAMAITFSRWYTWFGIIFITPAVYFFSTTWNPD
                     LLRRKLKWIWCHYLTAAVFYAVCVSSPHIVSGMYHYRAGYFPKAGALELVFIVWFYTV
                     MILSFLNFIRIYREATTTLLKRNMKLMFLAFVIAFFGSVEYLPNYGIPVFPVAFLPMF
                     LFTTIMGYCVIRYRLMDIETVIHKTFMWFVTSAVAILPFGGIIYTAQPMMRDLSPFAA
                     GVYFLTVLFIFYFYYRSVQPRLDKLFQRKHTNLMAVLDRFSGELVHLKNLRDLLQSFS
                     RLLRRELFVRRLSVYLRDETTGKMVPAIAKGIRNLRPVSLESSFLQWLTETDSAVVGE
                     LAVSDPSVEVFKVEMERLFLSTQAEVILPFILGGRLIGFASLGKKSNLRRYSFNEIRF
                     LDRLKVPATIAFSNSMRYEDIRKLSDELQLWNQALEERVERRTRELAKAQEQLVQAEK
                     LATLGTLAGGVAHEINNPLTAVLTNAQILRMSANEEDLESISLIEEGAKRCQGIIQKL
                     MKYARKTTEEMPHKDVSLVDVVKGTVALLGYQFHQENIEVLTRLDEGTPTVKGNAGEI
                     EQVLTNLLVNAKDSIRLAKVKGRIVIETRRTPKGVELCVSDNGPGISPENQKKIFDPF
                     FTTKDVGEGTGLGLSVSFGILKRHGATIRVASETGQGATFTMEFPV"
     gene            complement(2793..5447)
                     /locus_tag="PK590_03190"
     CDS             complement(2793..5447)
                     /locus_tag="PK590_03190"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOG23635.1"
                     /translation="MKVLVTGGTGFIGSTLVRHLIAAGHEVRVLVRQKKDSFLLQHLD
                     VEVVNGDVTNLPAVEEAVQGCSVVFNLASLYAFFPFWNKCPRAIYKINVGGTTHMLSA
                     ALKYGVKKFIHTSTIAALSAKPGRLPTDETSGFDFRGASHYARSKYLAEQEVLKYCGM
                     GLPAVILNPAIVVGERDYKPTPTGDVIVKFLNRSYPCYFDTCWTLADADDVAQAHLAA
                     VERGRSGERYILSASEPVTLREIFQKLEKLTGIRAPRLRVPYGILFGMTYLDELFSHY
                     VFRKKPLMPTEGVRFCKATGFYDNSKAIRELGYQPTPLEKTLRKAVDWYRKNGYVEAR
                     GFFRIRANGSNIISKVMRKLGLSGYTDKLNPGTLFFYAIVKMLMWLKKLGMGRARDGW
                     RGVAQCYLRTEQAKFGLAAFNLDLWSDFQGFHEKTFENARRHAMERLAKFIRGQSLFR
                     QGLRWSLFCARSYPRTFADLVAAEFDEKGFWVRLEPWMDPERGDFEEMPAETKALLLA
                     GLMEILNQTKDIADKKRPLVIKKKWRRWFSRHRDQIPAAWREDAEDYGRRILGASFIC
                     FEVLPQERGSAGESDRFRVPFFTQYRLPGCGTLAIVCRFTPAFDGADLWFHFSHVSMD
                     GVPSQEVLNELKRQWGTRGDFLFPSSGFRAGKIPDLCSLDGKNEICHAYQFLDFQPFL
                     KVRNDLNKRFGRRAKQVISSAALLIWKLAQYREFEDVKFAVPVDLRATPERERTLGFI
                     FIRPSIYLDKHRSDRGFFAFQQEFNRQLFATRKRRSESYRLLESYTVVPPAFYAVTSK
                     CLMRPLQEFTGTLGITIIKKADFFVAPASDIHTDGFIAFSNFSLPSAGGGKVGVVSMK
                     GPKEKIQGYMDVLRDIMQRAIQHDELYF"
     gene            complement(5516..7135)
                     /locus_tag="PK590_03195"
     CDS             complement(5516..7135)
                     /locus_tag="PK590_03195"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOG23636.1"
                     /translation="MNANPADNIGLEVRMSGSENVSFIKVSRVLPVRRWQALRLIARV
                     EDFPALVPAIKECRIIDRDYRRTITSWAVEMDGIPIRWREEALFDYKNFIVRFKALTG
                     DLESLEGHWSFLEGPSGTTEVVVEAAVRIGVPIMDQMVGGMITEKIRGVFESLLRFFE
                     ESIIDKRYQKMDSRGANAVSGFGVIGHPYNFQHLIQYLRSLNPNFNPPSPGFLGKVFD
                     LMPAYVSYDIKEFRSATGAVTRGAFISCNIIPEMLALDIENVIRKVVDSCKVAERQGL
                     GIVALGGFTSIAGERYGDVFLKQVRIPVTTGNTLTAALVVEGVVKAARLMGLELSKAR
                     VTVIGGSGDIGSACARALAAMTREVTITSRSSDNLKKMEKVIKAVRHAKFRGSHDNNE
                     AVRHADIVVAAASAPQSIVDISSFKSGAIVCDVGYPKNIAYAETDRNDILIFAGGICE
                     IPSEFNPGFDIGLSSPRVLYGCFSEAMVLALEGRYENFSWGKGRITREKMDEILMLAK
                     KHGFCLAPFFWGRRVLSDAEVAGIGKTAGRI"
     gene            complement(7293..7898)
                     /locus_tag="PK590_03200"
     CDS             complement(7293..7898)
                     /locus_tag="PK590_03200"
                     /inference="COORDINATES: protein motif:HMM:NF015390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOG23637.1"
                     /translation="MKMFKFAIALPLVCLMVLGFGVSLPNAFAADELVIADFDTGDKP
                     NNIGGDFGGWDKDPNDDTQGTVMSFATDDAKGDPAGYSIRLDYDVDSPNPAYNGFWMK
                     MNGEDATPYNTLCFYIKGDGKIGYTKRVKLELKDMSGKASPYIVSDITEQWQKVCIPF
                     EKFRRIENWSSLNEFVVVFDDINSNPKTGTIYIDQVSLANQ"
     gene            complement(8079..>8457)
                     /locus_tag="PK590_03205"
     CDS             complement(8079..>8457)
                     /locus_tag="PK590_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23638.1"
                     /translation="NPFQILSEVSHQVIMRVTLVLIATLMIIGGYGIAFLHRVAGPMF
                     RIRQTLLRLNRGESVPDIKLREGDFFHEMAHDLNVLIKHSRLTQEKLSEMKNGLNEIS
                     RSASSDEVRGKVQRLKELVDSIS"
BASE COUNT         2276 a         2374 c         1882 g         1925 t
ORIGIN      
        1 actgaaggag tgggtcctcc gcgctcaatt cgagagagag aaactgctta tcgtaccctc
       61 gctgacacac aagataaaaa tcagggcctc gaacgccccg gatcagaatc gccgcgttct
      121 tgacccgcat tttcatggta ataaaatgga gaatcaatcc ggcaagatga ttcagtgatt
      181 caacgctgga cattccccgt gacgcgtctt tcaggagttt ttgataatcc ccttcgtcgg
      241 aaaacagata tcggtccatc acataaccaa gaaatttccg aacaggaaca taaagcgaaa
      301 gaacgacccc aacgctcaaa atactgttca cgattgctcc ggcaaatccc caccgtccgg
      361 tcaccctttg aactaaaatt gccaccagca ccgataccgc ggccaaaatc acaaagagag
      421 aaacagacac tgttattttt ttaaccataa catccaccac aaaacatcag aaggttcgac
      481 accgccgact ttgttttcta tactaaaaaa aatcagctcc ggatcatttc acctcaaatg
      541 acaaccggga gccaccgcat caaaacgtat ttccctttgc ctttgtatcg gatttctccc
      601 gcgcaactgt aacgctttag acttatcaca caggaaattc catcgtaaac gtcgcgccct
      661 gtcccgtctc tgaagcaacc cggatcgtgg cgccgtggcg cttgagaata ccaaaactga
      721 cagaaagccc aagccccgta ccttccccga catccttggt cgtaaaaaat ggatcgaaga
      781 tttttttctg attttccgga gaaattccgg gaccgttgtc gctaacacaa agctcaaccc
      841 cttttggagt acggcgggtt tcgatcacaa tccgcccttt gaccttggcc agccggatag
      901 aatccttggc gtttacaagg agattggtga gtacctgctc gatctcgccc gcgttgcctt
      961 tcacggtggg cgttccctca tccagccgcg tcaagacttc gatattttcc tgatgaaact
     1021 gatagcccag caaggccacc gtccctttta ccacatcgac aagactcacg tccttatgcg
     1081 gcatttcttc tgtggtcttc cgtgcatatt tcatgagctt ctggataatt ccctggcatc
     1141 gtttcgcgcc ttcctcgatc aaggaaatcg attcgagatc ctcttcatta gcgctcatcc
     1201 ggagaatttg ggcattggtg agcacggccg taagaggatt gttgatttca tgcgccaccc
     1261 cccctgccag cgtcccaagc gtcgcgagtt tctccgcctg gacaagctgt tcctgcgcct
     1321 tggcgagttc ccgcgtcctg cgttccaccc gttcctccag cgcctggttc caaagctgaa
     1381 gttcatccga aagtttccgg atatcttcat agcgcatcga gtttgagaaa gcgatcgtgg
     1441 ccggcacctt taaccgatca agaaaacgga tttcattaaa actgtaacgc cgcaaattgc
     1501 tttttttgcc aaggctcgcg aacccgatca aacgaccgcc caggataaaa ggaaggatca
     1561 cctcggcctg tgtcgacaaa aaaagacgtt ccatctccac cttaaaaact tcaaccgaag
     1621 gatcgctgac cgccagttcc cccaccacgg ccgaatccgt ttcggtcagc cattgaagaa
     1681 aagaactttc cagcgacact ggccgcaggt ttcggattcc cttcgcgatc gcgggaacca
     1741 tctttccggt cgtttcgtca cggagataaa ctgaaagacg gcggacaaaa agctcgcggc
     1801 gaaggagccg ggaaaaactc tggagaagat cccgtaagtt tttgaggtgc accagctctc
     1861 cggaaaaccg gtcgagcacc gccatcaggt tcgtgtgctt gcgttgaaac agtttgtcga
     1921 gccgtggctg aacggatcgg tagtaaaaat aaaaaataaa aaggaccgtc agaaaataaa
     1981 cccccgcggc gaacggtgac aaatccctca tcatcggctg agccgtgtaa ataatccctc
     2041 cgaacggcag gatcgcaaca gcactcgtca caaaccacat aaatgttttg tgaatcaccg
     2101 tttcgatatc catgaggcgg taacgaataa cacaatatcc cataatggtt gtaaaaagaa
     2161 acatcggcag gaaggcgacg ggaaataccg ggattccgta attgggcaga tattcaactg
     2221 atccaaaaaa cgcgattacg aaagcgagga acatcagctt catgtttctt ttgagaaggg
     2281 tcgtcgtggc ctcccggtaa atccggataa aattaaggaa cgacaggatc atgaccgtgt
     2341 agaaccacac aataaatact agttccagcg ctccagcctt cgggaaatag ccggcgcggt
     2401 aatgatacat ccccgaaaca atatgcggcg aagacacgca gaccgcgtag aaaacggccg
     2461 ccgtcagata atggcaccag atccatttca gcttccgcct caaaagatca ggattccatg
     2521 tcgtgctaaa aaaataaacg gccggcgtga taaaaataat tccgaaccag gtgtaccagc
     2581 gtgaaaacgt aatcgccatg gcctcatccc tcattgaaca ggctacgcct actcccgtaa
     2641 gccagattcc cgccgtcacc gtcgtgaccg tatacgcaat gtgggttatt gtttttttac
     2701 cctgaaaaaa aacgaaaagg ccttcaaacg aaatcaggac gccgacgata aagtgcggca
     2761 tcgcgtagcc gttcagataa taattctggg gattaaaaat agagttcatc atgctgaatc
     2821 gccctttgca taatatcacg gaggacatcc atgtacccct ggatcttttc tttcggcccc
     2881 ttcatgctga ccaccccaac cttcccccct ccggcggaag gcagggaaaa attactgaaa
     2941 gcgataaacc cgtccgtgtg gatatcgctc gccggcgcga caaaaaagtc cgcttttttg
     3001 ataatcgtga tgccgagcgt tcccgtaaat tcctggagcg gacgcattaa acacttggac
     3061 gtcaccgcgt aaaacgccgg aggaaccacc gtataggatt caagaagccg gtaactttcg
     3121 ctccgccttt tccgggtcgc aaaaagctgg cggttaaatt cctgctgaaa cgcgaaaaaa
     3181 ccgcggtcag aacgatgttt gtcaagataa atggacggcc ggataaagat gaacccgagc
     3241 gtcctctccc tttcaggggt cgctcgcaaa tccacgggaa cggcaaactt gacgtcctcg
     3301 aattcccggt attgcgccag tttccagata agcagagccg cggaagagat tacttgtttc
     3361 gcgcgccgcc caaatcgttt attcaaatca ttccgaactt tgagaaaagg ctgaaaatca
     3421 agaaattgat aggcgtgaca aatttcattt ttgccgtcca gggaacaaag atccgggatt
     3481 ttccctgccc ggaaacccga agacggaaac aaaaaatcac ctcgcgttcc ccattggcgt
     3541 ttgagttcat tcaaaacttc ctgagaagga acaccatcca tggaaacatg gctaaagtga
     3601 aaccagagat ccgccccgtc aaaagcggga gtgaatcgac aaacaatggc aagcgtcccg
     3661 caaccgggaa gccgatattg agtaaagaaa ggcacccgaa aacggtccga ttctcctgca
     3721 gagccccttt cctggggaag cacttcaaaa caaataaatg aagcgcccaa aatccgtcgt
     3781 ccataatctt cggcgtcttc cctccatgcg gcagggatct gatcccggtg tcttgagaac
     3841 cagcggcgcc actttttttt gatgaccagc ggtctctttt tatccgcaat atcttttgtc
     3901 tggtttagaa tctccatcaa tcccgcgagg agcagcgcct tcgtctccgc cggcatctcc
     3961 tcgaaatccc cacgctccgg atccatccac ggctccaggc gcacccaaaa acctttctca
     4021 tcaaactctg ccgcgacgag atccgcgaaa gttcgcggat acgacctcgc gcaaaacaaa
     4081 ctccaccgga gtccctgccg aaacagggat tgtcccctta taaatttcgc cagccgttcc
     4141 atggcatgac gccgagcgtt ctcaaaagtt ttctcatgaa atccctgaaa atctgaccag
     4201 agatccaaat tgaaagccgc caggccaaac ttggcttgct cggttcgaag ataacattgc
     4261 gcgacaccac gccaaccgtc cctggcgcgc cccatgccca acttcttgag ccacataagc
     4321 attttcacaa tcgcgtaaaa gaagagcgtc ccgggattaa gcttgtccgt gtaaccactg
     4381 aggccgagct ttcgcatcac tttcgagatg atgttcgaac cgttcgcgcg gatacggaaa
     4441 aatccccgcg cctcgacata gccgtttttc cggtaccagt ccaccgcctt ccgcagagtt
     4501 ttttcaaggg gggtgggttg ataacccagc tcacgaatcg ccttggaatt atcataaaaa
     4561 cccgtcgcct tgcaaaaccg gacaccttct gttggcatca gaggtttttt ccggaacacg
     4621 taatgagaaa aaagctcatc cagataagtc atcccaaaaa gaattccata aggaactcga
     4681 agtcgaggcg ccctgatccc ggtaagtttt tcgagttttt gaaaaatctc ccggagcgtg
     4741 acgggctccg aagcggaaag aatatatcgt tcaccgctcc gtccgcgttc aaccgcggcc
     4801 agatgcgctt gggccacatc atccgcgtca gccagcgtcc aacaagtatc aaaatagcag
     4861 ggataactcc ggttcagaaa tttgacgatc acatcccccg tgggagtcgg tttgtagtca
     4921 cgctccccga caacaatcgc ggggttcagg atgaccgcgg gaagacccat tccgcagtat
     4981 ttcagcactt cctgctccgc cagatacttt gaacgagcgt agtgactcgc tccacggaaa
     5041 tcaaaaccgg atgtctcgtc cgtggggagc ctgccgggtt tcgcgctgag ggccgcaatg
     5101 gtactggtgt ggataaattt tttaacgcca tacttgagcg ccgcggaaag catatgagtg
     5161 gtaccgccca cgtttatctt gtagatagct ctcgggcatt tgttccagaa cgggaaaaac
     5221 gcgtaaagcg aagcgaggtt aaaaacgacc gagcatccct gcacggcttc ttcgacggcc
     5281 ggaagattcg tcacatcgcc gtttacaacc tcgacatcaa gatgttgcag gagaaaactg
     5341 tcttttttct gacgaaccag cacgcgcact tcatgaccgg cggcaatcaa atgccgcact
     5401 agcgtggaac cgataaaccc cgtaccaccc gtaaccagga ctttcattgt ttcaggcgcc
     5461 tgtccgcttt gtgtccctga agacaagaaa tgcgcgcatt gggactccgc attgtttata
     5521 ttcgccccgc tgtcttcccg atcccggcca cctccgcgtc cgacaggacg cggcgccccc
     5581 agaaaaatgg cgcgaggcag aacccgtgtt ttttcgcgag cataagaatc tcatccattt
     5641 tttccctggt aatgcgccct ttcccccatg aaaaattctc gtatcgtccc tccagggcaa
     5701 gcaccatcgc ctccgaaaaa cacccgtaca gaacccgggg agaggaaagt ccgatgtcaa
     5761 atccgggatt aaactcggac ggaatctcac aaattcctcc ggcaaaaatc aggatgtcgt
     5821 tccgatccgt ttcggcatag gcaatatttt tcgggtaccc gacatcgcac acaatggcgc
     5881 ccgacttgaa gctggaaata tcaacaatgg actgcggcgc gctcgcggcc gcgacgacaa
     5941 tatcggcgtg gcgaaccgct tcgttattgt cgtgactccc tctaaacttc gcgtggcgga
     6001 cagccttgat caccttctcc atttttttta aattatccga acttcggctt gtgatcgtca
     6061 cctctctggt catagccgcc agcgcgcgag cgcacgcgct tccgatatca cccgatccac
     6121 caatcacagt cacccgcgct ttcgaaagct ccaaccccat gagcctggcc gctttgacga
     6181 ccccctcaac cacgagggcc gccgtcagcg tatttccggt cgtgacagga atcctaactt
     6241 gctttaaaaa aacgtctcca tagcgttcac cggcaatcga cgtaaatccg cccaaggcta
     6301 caatcccaag tccttgccgc tccgccacct tacaggaatc gaccactttt cgaatgacat
     6361 tttctatatc cagcgcgagc atttcgggaa tgatattgca ggaaataaaa gccccccggg
     6421 tcacggctcc agtcgccgat ctgaattcct tgatatcata ggaaacatag gccggcatga
     6481 gatcaaaaac ttttccaaga aatcccggtg aaggcggatt aaaattcgga ttcagcgagc
     6541 gtaaatactg gatgaggtgc tggaaattat aaggatgccc gatcacgcca aaccccgaga
     6601 cggcatttgc cccccgagaa tccatttttt gataacgctt gtcgatgatc gattcttcaa
     6661 aaaaacgaag tagcgattcg aaaacccccc ggatcttttc cgtgatcatt ccgccgacca
     6721 tctgatccat gatcgggaca ccaatccgga cggccgcctc caccacaacc tccgtggtac
     6781 cggaaggccc ctcgagaaag ctccaatgcc cctccaaact ttccaaatca cccgtcaggg
     6841 ccttaaatcg cactataaaa tttttataat caaagagggc ttcctcccgc caacgaatgg
     6901 gaatgccatc catctcgaca gcccaggaag ttatggttcg ccggtaatcc cggtcaataa
     6961 ttcgacattc tttaattgct ggaaccaggg cgggaaaatc ttcaacccga gcgataaggc
     7021 gcagtgcctg ccatcgcctt acgggtagaa cacgacttac cttgatgaag gaaacatttt
     7081 cagaaccgga cattcgaacc tccagtccga tattatcggc cggatttgcg ttcacttcac
     7141 ttccgagaaa gacttcaaaa attcccttct aacacgtcag aatccttgtg aacaggtacg
     7201 aactaggacc ctccgacata cgcggttgtg cttataaaaa aatagccccg aggccaacag
     7261 gccacggggc tattcttaaa cgaaattctg aattactgat tggccagaga aacctggtca
     7321 atatagatgg taccggtctt gggattcgag ttaatatcat cgaacaccac gacaaattcg
     7381 ttcaaagagc tccaattttc aatacggcgg aacttctcaa acgggatgca gaccttctgc
     7441 cactgctcgg taatatcgct cacgatatac ggtgacgcct tgcctgacat atccttgagc
     7501 tcgagcttca cacgcttcgt gtatccgatt ttaccgtcac ccttgatgta gaaacaaagg
     7561 gtgttgtagg gagtcgcgtc ctcgccattc atcttcatcc agaacccgtt atacgccggg
     7621 tttggagaat ccacgtcata gtccagacgg atcgaataac ccgccggatc acctttggca
     7681 tcatccgtgg caaatgacat cacggttccc tgggtatcat cgttcggatc cttatcccat
     7741 cccccgaaat caccaccgat attattcggc ttgtcacccg tgtcaaaatc ggcgatcaca
     7801 agctcgtcgg cagcaaaagc attcggcaga gaaacaccga accccagaac catcaaacaa
     7861 actaggggta aagctattgc gaatttaaac atcttcattt tctgttgact cctttcgtgt
     7921 aataaactta attggcgagg gtcgacgaga gtatacacgc ttataaaagt atttacaaga
     7981 aaagttgaga tagcataata gatcaatttt atcattaaaa aaaattaata tttttgttct
     8041 tttcttgtcg ccatcacgcg gcttctgttt tcttatactt aagaaatcga atcaacaagc
     8101 tccttcaagc gctggacctt tccccgcacc tcatcggagg acgccgaccg agaaatctcg
     8161 tttaatccat ttttcatctc ggaaagcttc tcttgagtca atcgagagtg ttttatcaaa
     8221 acattaagat cgtgagccat ttcatggaaa aaatcacctt ccctgagttt gatatcagga
     8281 accgattctc ctctgtttaa cctaagaagt gtttgtcgaa tccggaacat cggacccgcg
     8341 acccggtgaa gaaacgcgat cccgtagccc ccgataatca tcaaagtcgc gatcaatacc
     8401 agggtcacgc gcataataac ctgatggcta acctctgaaa gaatctggaa gggattg
//
LOCUS       DAOSIO010000055         8455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_7833_length_8455_cov_4.114167, whole genome shotgun sequence.
ACCESSION   DAOSIO010000055 DAOSIO010000000
VERSION     DAOSIO010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 8455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8455
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7833_length_8455_cov_4.114167"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..502
                     /locus_tag="PK590_03210"
     CDS             98..502
                     /locus_tag="PK590_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG23639.1"
                     /translation="MLKKDADDFELNFFEGVVRERPNYVEALVPLAHVYTRLGFHEKS
                     LEIDKRLAGLCKHDPVIHYNLACSYSLLGQLPQSLTALKKAVRLGFQDLRYIEKDQDL
                     KNLCTYRPFLRWMETVKASQFTKMLGKIQERS"
     gene            502..1614
                     /locus_tag="PK590_03215"
     CDS             502..1614
                     /locus_tag="PK590_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOG23640.1"
                     /translation="MEKGVPEHLTAKNGRKLLRFLEGRGEALSPLLIVMHNYPDPDAI
                     ATAFTLQYIAEKLCNIKSRIVYKGSIGRVENRAMVSQLRIPIHKIHASDISKYNHVAL
                     IDTQPAFENNPYPRDRPVTMVIDQHPYVTKPTAELVIVDTECGATSVILAQALLLSGL
                     EIPKRIATALAYGILSDTLNLYRAKRADIIETYLAILQLCDMRALIKIQHPPKSRKFF
                     TTLSKGLGVAKAVRGLIVSHLGKVEHPDLVSEIADFLLTYKGMERSLCTGRFKGKLHV
                     SLRSNRPTDDTPEILRNIFNDRKEAGGHGMVAGGAFDVSEDEKDEPRWQEAENTLVQR
                     LRKRLRIPVKCESYSPFRTAEPSAGSAVSKDFPAPC"
     gene            1690..2286
                     /locus_tag="PK590_03220"
     CDS             1690..2286
                     /locus_tag="PK590_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOG23641.1"
                     /translation="MRAFLLTLVVIIIIALVVTGFMIASVYNDLVVKEEGVNGAWAQV
                     ENVYQRRADLVPNLVEAVKGYVAHEKETLQNVVNARAQIGKMDTSSKLLNSPDLLAKF
                     QDAQTQLSSAIGRLLVVVEQYPDLKASQNFLALQNQLEGTENRITVERRRFNESARDY
                     NAFRRQFPNCWVAQMAGFQNKAYFQTDQDARTVPQVKF"
     gene            2317..3075
                     /locus_tag="PK590_03225"
     CDS             2317..3075
                     /locus_tag="PK590_03225"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOG23642.1"
                     /translation="MSRGSDRSLLLGVGRYAFCRKFLVFFLGAFLSISFVSAFEIPSR
                     PDGYVTDRAGLLSPTAKIELEKKLSDFDLETSNQVAVAVFSSLEGENLEDISMRIVEA
                     WKPGRKDRDNGVLLLIFEKERQIRIEVGYGLEGALTDAVSGQIIMGVMAPLFKQGNYD
                     QGVLEGANAVMAAIQGEFEGGPIEENSYVPQIMFLIFIFLYLWFLSQPSRIGRRRGIH
                     GGFWIGGGPGRGGFGGGFRGGGGGFGGGGASGRW"
     gene            3154..3600
                     /locus_tag="PK590_03230"
     CDS             3154..3600
                     /locus_tag="PK590_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOG23643.1"
                     /translation="MKFLKYFPVFFFSESEKKIIEESIGEAEKHTSGEIRVHLEGRVR
                     GDILVHARKVFEWIGMTKTKDRNGVLLFLSVRDRQVAILGDRGINEKVPPKFWEQEIA
                     LIQESFSRGEFAEGVSKAVLQVGYKLKEYFPRDRTDANELPDKISF"
     gene            3657..3779
                     /locus_tag="PK590_03235"
     CDS             3657..3779
                     /locus_tag="PK590_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23644.1"
                     /translation="MTVSGNLVERFAVTDSVKSGPGTATRKDDLGIGTSAAYSF"
     gene            3825..4358
                     /locus_tag="PK590_03240"
     CDS             3825..4358
                     /locus_tag="PK590_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23645.1"
                     /translation="MGIHESWEKALKMTEIVRPRVQPLATFESTQIPYIFLAESLVNH
                     GDTIIRKGEITVDKPSIILPPYMPQLEGFEFEKNFAGASDYLTTFLLMRGIRFPSMKY
                     ENKVNTLDVHEGKLRQAIDKYSKILQQQENVTTGLVIGPEDCWQFSVLIFVCTQALRS
                     AEGDIQRIFERLKDGEK"
     gene            4360..6201
                     /gene="malQ"
                     /locus_tag="PK590_03245"
     CDS             4360..6201
                     /gene="malQ"
                     /locus_tag="PK590_03245"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOG23646.1"
                     /translation="MKEGTHYASFTSSACAKAWRKVGARRRAGVATPLFSIYSKSSIG
                     IGEIPDLKRLVDWCVKTGISLIQLLPLNDVAFDFRPYDAQSSTALDPMYLSLDHLVSC
                     DLKLFSKEVAVLRRKFPAGKGRVNYEVKAAKLDLLKKIFSHQSTDLKDKKFSTFRKKQ
                     SGWLSDYAVFKALKVKFNAAGWMDWPEEFRNRNSGVMDQVEKERADEILFNQWLQWQL
                     YQQLSEAKHYATGLGVLLMGDMPFLVSRDSSDVWANQDYFKLDLSSGAPPDLYFAKGQ
                     RWGMPPYHWERIAADGYKYLTRKMQYLENFFDLYRVDHFVGMFRLWTIPVSEPAERAG
                     VAGTFDPPVESDWKEHGRRILDVMTRETTMLPCAEDLGTVPKCSYEILDEYKIPGSDV
                     QRWVRNWNTDMTFKSADHYRPNGMAMLSTHDTSAFCAWWEYEAGTVDEEFFAKKCAEA
                     GVDFDQVKAWLFDLDASVHGRSRWKTEISDLEKFLSVIGRKKEEAWHLAEAFLGTYNE
                     RDLYWKYLGLEGVPAKKASGLLVRKALEAVNGTCSVFSLQLLQDWLALENYPGRNDWE
                     YRINVPGSMASSNWSLVSPFSLEKMMTLPMNRDIRAIVARTNREVSK"
     gene            6198..6953
                     /locus_tag="PK590_03250"
     CDS             6198..6953
                     /locus_tag="PK590_03250"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOG23647.1"
                     /translation="MIRLFLNKTNLRYIVTGVLLTSLLVLSVLFIKQRKVHELATQPR
                     ALPSVSYYPVPKSMGPDSAPIKIIEYSDFECPSCRFATEVLHQLFATYSGKIQLVYHH
                     YPLTGHPWSAHAHQAAECMHIQGKFWPYHDMLYRNQREWAAMPEAPVPKLMEYAEGCG
                     ANMELFLNCMADPAVTSGIYAEKNEGTLRQVNATPTFFMGDKRYVGARELQERGENDI
                     RRVLGLPELSFSAPPDPQVDQTPKPPTAVPGGE"
     gene            6950..7414
                     /locus_tag="PK590_03255"
     CDS             6950..7414
                     /locus_tag="PK590_03255"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HOG23648.1"
                     /translation="MMKRESSYTILFLGRLLIGLIFAYSGYTKLVEPVENFQAAIAAY
                     EIIPYVFIPWIARLIPWIEFIFGVFVIVGYLTRISAAMLAAMSWSFVMVIALTWLVTG
                     SIPADCGCFGEGSFVHFPAYQIFILDVISTLIGIRLCLMRSHPLSLDALLGK"
     gene            7543..7615
                     /locus_tag="PK590_03260"
     tRNA            7543..7615
                     /locus_tag="PK590_03260"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7576..7578,aa:Phe,seq:gaa)
     gene            7779..8243
                     /locus_tag="PK590_03265"
     CDS             7779..8243
                     /locus_tag="PK590_03265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOG23649.1"
                     /translation="MRYTTKTEYGVICLAYMAMPGRTVGSTAKEIAECGGFSVPFTEK
                     ILQKLRKTGMVSSQQGNRGGWTLARAPREISLKEIVEALEGSTFETFCDSKRRGSMEC
                     RHFSVCKLKPIWYRTKDTLDNLYEKITLDQIARDLMGSKSSRFLERKRKERS"
     gene            8240..8437
                     /locus_tag="PK590_03270"
                     /pseudo
     CDS             8240..8437
                     /locus_tag="PK590_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202967.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
BASE COUNT         2023 a         1905 c         2311 g         2216 t
ORIGIN      
        1 taaagcaatc cgtcgaacgt ttaggggtcc ggaattttgt tcttaaacct ttcgtagcgg
       61 atgaattttt aagggttttg gaaaggagtt taaacccatg ctgaaaaaag acgcggatga
      121 ttttgagcta aacttctttg agggggtggt gcgggaacgt ccgaattacg ttgaagcgct
      181 tgttcctttg gcgcatgttt acacccgcct tggatttcat gaaaaaagtc tcgagattga
      241 taaacgtttg gccggtcttt gcaaacacga cccggtgatt cattacaatc tggcttgcag
      301 ttattctttg ctgggacaac ttcctcaatc tttgaccgca ctcaaaaaag ctgttcgcct
      361 tggatttcaa gaccttcgat atattgaaaa agatcaggat ttaaaaaatc tctgtactta
      421 caggcctttt cttcgatgga tggaaactgt caaggcctca caattcacga agatgttggg
      481 aaaaatccag gaacgttcgt aatggagaag ggagttccgg aacaccttac cgcaaaaaac
      541 ggacgtaaac ttcttcgctt tcttgaagga cgcggagagg ccctttcccc gcttttgatc
      601 gtgatgcaca actatccgga ccctgatgcg atcgcgacgg cttttacgtt acaatatatc
      661 gccgaaaaac tctgtaatat caaatcccgg atcgtctaca aggggagtat cggacgagtt
      721 gaaaaccgtg ccatggtcag tcagcttcgt attcccatcc acaaaattca cgcttcagat
      781 atctccaaat ataatcatgt ggcgctgatt gatacccaac ccgcctttga gaacaatccc
      841 tacccgaggg accgcccggt taccatggtt atcgaccagc acccgtacgt gacgaagccg
      901 accgcggagc tggtcattgt tgacacggaa tgtggcgcga cgagcgtcat actcgcccag
      961 gcccttcttt taagcggact ggagattccg aaacgaatcg cgacggccct tgcttacggg
     1021 attttgtcag acacgctcaa cctttatcgc gcgaaacgcg ccgacatcat cgaaacctat
     1081 cttgcgattc tccagctttg cgatatgagg gcgctcatca aaatccagca tccgccgaaa
     1141 tctaggaaat ttttcacaac tctcagtaaa gggctgggtg tcgcgaaagc ggtccggggt
     1201 ctgatcgttt ctcatcttgg gaaggttgaa caccctgacc tggtttcgga aattgcggat
     1261 ttcctcctga cctataaggg gatggagcgg tctctttgta cggggcgttt caaagggaaa
     1321 cttcatgtgt cgcttcggag caatcgtccg acggacgata ctcccgaaat tctccggaat
     1381 atttttaatg acagaaaaga agccggggga catggcatgg tggccggagg cgcgtttgat
     1441 gtttcggaag atgaaaagga tgaacctcgg tggcaggagg ccgaaaatac gcttgttcag
     1501 cgtctgcgga aacgtcttcg aattcctgtg aaatgtgaga gctactcgcc tttccggacg
     1561 gcggaacctt ctgccggctc cgccgtttcg aaggattttc ccgctccttg ttagctattg
     1621 cgggttttgg gcggggaact ataataaaat tacgaagtat ttaacctatt catttggagg
     1681 gtttaaatta tgagggcatt tctcctgact ttggttgtga ttatcatcat tgcgcttgtg
     1741 gtgacgggtt tcatgatcgc gagtgtttac aatgaccttg tggtcaagga agagggcgtg
     1801 aacggcgcct gggcgcaggt tgaaaatgtg tatcagcgcc gcgcggatct tgttcccaat
     1861 cttgtggaag ccgtgaaggg atatgtggcc cacgaaaaag aaactctcca gaatgtggtc
     1921 aatgcccgtg cccagatcgg taaaatggat acgtcttcaa aacttctgaa tagccccgat
     1981 cttctggcga agttccagga tgcccaaacg cagttgagct ccgcgatcgg ccggttgctg
     2041 gtggtcgtgg aacaatatcc ggatctcaag gcgagccaaa attttctcgc tctccagaac
     2101 cagcttgagg ggaccgagaa ccggattact gtcgagcgcc ggcgtttcaa tgaatctgcc
     2161 cgggactata atgcttttcg ccgtcagttc ccgaactgtt gggtcgccca aatggccgga
     2221 tttcagaaca aggcctattt tcagactgat caggatgcca ggaccgtgcc ccaggtgaag
     2281 ttttaattat ccgggtcgta aggctcgaag tttaaaatgt cgcgaggttc tgaccgatct
     2341 ttgctgttgg gcgttggccg ttacgcgttc tgccgcaagt ttctcgtttt ttttctgggc
     2401 gcgttccttt caatatcgtt tgtctctgct ttcgagatac cttcccggcc tgacggctat
     2461 gtgacggatc gcgcggggtt attgtctccc acggcgaaaa ttgaactcga aaagaagttg
     2521 tccgattttg accttgaaac ttccaaccag gtcgccgtcg ccgttttttc ttctctcgag
     2581 ggagaaaacc ttgaagatat ttccatgcga atcgtggagg cgtggaaacc cgggcggaag
     2641 gatcgggata atggcgtgct tttgctgatt tttgaaaagg aacgccagat tcggattgag
     2701 gtaggctacg ggctggaagg agcattaacg gatgctgttt ccggccagat tatcatgggg
     2761 gtgatggccc cgctcttcaa acaggggaat tatgatcagg gagttcttga aggcgcgaac
     2821 gcggtgatgg cggcgatcca gggggaattt gaagggggtc cgattgagga aaattcttac
     2881 gtgccccaaa tcatgttctt gatatttatt tttttatatt tgtggttcct cagtcagccc
     2941 tcaagaatcg gacgccggcg gggaattcac ggcggcttct ggatcggcgg aggcccggga
     3001 cgcgggggtt ttggcggggg attcaggggt ggcggtggcg gatttggcgg gggtggcgcc
     3061 tccggaaggt ggtgagtgtt ctcctccggg gatgtgaatg gataaatctg aaaacaggat
     3121 ttttgataag ataaagcggg cggctgaaga accatgaaat ttttaaaata ttttccggtc
     3181 tttttctttt ctgaatcgga aaagaaaata attgaggagt ccattgggga agccgagaaa
     3241 cacacctccg gcgaaatcag ggttcatctc gagggacggg tccggggaga tatcctggtc
     3301 cacgcccgga aggtgtttga gtggatcggg atgacaaaga caaaagaccg gaacggggtt
     3361 ttgttgtttt tgtctgttcg tgaccggcag gttgcgattt tgggtgaccg gggaatcaac
     3421 gaaaaggtcc ctccgaagtt ttgggagcag gaaattgctc tcatccagga atccttcagc
     3481 cggggtgaat ttgccgaagg tgtttcaaag gcggtgcttc aagttggtta taaactcaaa
     3541 gaatatttcc cgcgtgaccg gaccgatgcc aacgagcttc ccgacaaaat ttcattctaa
     3601 tctcgcctga tcgccccggg attgaagttg tttttacaag cgataatcag ggtcagatga
     3661 ctgtatccgg gaatctggtc gaaagatttg ctgtgactga ctctgtcaag tctggccccg
     3721 gtacggctac ccggaaagat gacctcggga ttgggacttc agcggcatat tctttttaaa
     3781 ttttctaaag gggtatgcta tagtctccgt caacaggagt ttctatgggg attcatgaat
     3841 cttgggaaaa agcgttgaaa atgacagaga ttgtgcggcc gcgcgttcag ccgttggcga
     3901 cctttgaatc tacccagatt ccttatatct ttctcgcgga atctctcgtg aatcatggtg
     3961 ataccatcat tcggaagggc gagatcacgg tcgacaagcc ttccatcatt cttccgcctt
     4021 atatgcccca gcttgaaggt tttgaatttg aaaagaactt tgcgggggcg tcggattatc
     4081 tgacgacgtt tctcctgatg cgcggtatcc ggtttccatc gatgaagtat gagaacaaag
     4141 tcaatacgct ggatgtccat gaaggcaaac ttcggcaggc gatcgataaa tattcaaaaa
     4201 ttttacaaca acaggaaaac gtgaccacgg ggcttgtgat cgggccggag gattgctggc
     4261 aattttcggt acttattttt gtctgcaccc aggccctccg ctccgcggag ggcgacattc
     4321 aaagaatatt tgaacgtctt aaagacggag aaaagtgaaa tgaaagaagg aacccattac
     4381 gcatcattta cgagctcggc ctgcgcgaag gcctggagaa aggttggcgc ccggaggagg
     4441 gcgggcgtcg cgacaccgct tttttccatc tattcaaaat caagtatcgg gatcggcgaa
     4501 atccccgacc ttaagcggct tgtggattgg tgcgtcaaga ccgggatttc actgattcag
     4561 ctactgcccc ttaatgatgt ggctttcgat tttcgtccgt acgacgccca gtcaagcacc
     4621 gcgcttgatc ccatgtatct ttctctcgac catttggtga gttgcgacct caagcttttt
     4681 tcaaaagaag ttgccgtgct tcgtcgaaag ttccccgcgg ggaaggggcg ggttaattac
     4741 gaagtgaagg cggccaaact ggacttgctt aaaaagattt tttctcatca atccacggat
     4801 ctcaaagaca aaaaattctc caccttcaga aaaaaacagt cggggtggct ttccgattat
     4861 gctgttttta aggcgcttaa ggtaaagttt aacgcggcgg gctggatgga ctggccggag
     4921 gaatttcgta acagaaattc tggtgtgatg gatcaagttg aaaaggaacg cgcggatgaa
     4981 attcttttca accagtggct ccagtggcaa ctttatcaac agttgtccga agcgaaacat
     5041 tacgcgaccg ggctcggagt gctccttatg ggagatatgc catttttggt ttcccgtgac
     5101 agctcggatg tatgggcgaa ccaggactat tttaagctgg acctttcctc gggcgcgccc
     5161 ccggaccttt atttcgcgaa agggcaacgc tgggggatgc caccctacca ctgggagcgc
     5221 atcgcggctg acgggtacaa gtacttgaca agaaaaatgc aataccttga gaattttttc
     5281 gacctttacc gcgtcgatca ttttgttgga atgttccggc tttggacgat tccggtttcg
     5341 gaacccgcgg aacgggccgg cgtcgccgga acttttgatc ctcccgtgga aagtgactgg
     5401 aaggaacatg ggcggcgcat tcttgatgtt atgacgcgag aaaccacaat gctaccttgc
     5461 gctgaagacc ttggaactgt gccgaaatgt tcgtatgaaa ttcttgacga gtataaaatc
     5521 ccgggctcgg atgttcaacg ctgggtccgg aactggaaca cggacatgac tttcaagtcg
     5581 gccgatcact atcgcccgaa cggaatggcc atgctttcca ctcacgacac aagcgcgttt
     5641 tgcgcctggt gggaatatga ggcggggacg gtggatgaag agtttttcgc gaaaaaatgc
     5701 gccgaggcgg gagtggattt tgatcaggtg aaggcctggt tgtttgatct ggacgcgtcg
     5761 gtccatggcc gctcgcgatg gaaaaccgag atttcggatc tcgaaaagtt tctttcggtt
     5821 atcggtcgaa agaaagaaga ggcctggcat cttgcggaag ccttcctcgg tacttataat
     5881 gagcgcgacc tttattggaa atatctcggt cttgagggag tgcccgcgaa gaaggcatca
     5941 ggtttgctgg tccggaaagc cctggaggct gtaaacggga cctgttcggt ttttagtttg
     6001 caacttcttc aagattggct tgccctggaa aattatccgg gaagaaatga ttgggaatat
     6061 cgtattaatg ttccggggtc catggcatcc tcaaactggt cgcttgtgag tcctttttcg
     6121 ctggaaaaaa tgatgacatt gcctatgaac cgggatatac gggcgattgt ggcccggaca
     6181 aaccgggagg tttcaaaatg atccggctgt ttttaaacaa aacgaatctt cgatatatcg
     6241 tgactggggt gcttctgact tcgttgcttg tgctatcggt tcttttcata aagcagcgga
     6301 aggtgcacga gctcgcgact cagcccaggg cgcttccttc ggtaagttat tatcctgtcc
     6361 cgaagtcgat ggggccggat tccgccccga tcaaaattat tgaatatagc gatttcgaat
     6421 gtccctcatg ccggttcgct acggaagttc tccatcaact gtttgctact tattccggaa
     6481 aaatccagct tgtttatcac cattatcccc tgacggggca tccatggtcc gctcacgcgc
     6541 accaggccgc ggagtgtatg cacattcagg gaaaattctg gccttatcac gacatgcttt
     6601 acaggaacca aagggagtgg gcggcgatgc ccgaagcacc ggttcccaaa cttatggagt
     6661 acgcggaagg atgcggggcc aatatggagc tttttttaaa ctgtatggcg gatcccgcgg
     6721 tgacgagtgg aatttatgcc gagaagaatg aagggacact ccgtcaggtt aacgcgaccc
     6781 cgactttttt catgggagac aaacggtatg tgggagcccg tgaattacaa gaaaggggcg
     6841 agaatgatat ccgaagagtt ttaggactgc cagagctgtc attttcggcg ccgcccgatc
     6901 ctcaggtgga ccagacgccg aaaccgccga cggctgttcc gggaggtgaa tgatgaaacg
     6961 tgaatcatcc tacacaattc tttttctggg ccgtcttttg atcggtttga tttttgctta
     7021 ctccggttac accaaactgg ttgaacccgt cgagaacttt caggcggcga ttgccgctta
     7081 cgagattatt ccctacgttt ttattccgtg gatcgcgaga ctgattccgt ggattgagtt
     7141 catctttgga gtttttgtga tcgttggata tttgacgcgt atcagtgcgg cgatgctcgc
     7201 ggcgatgtcg tggagcttcg ttatggtgat cgcgttgacc tggcttgtaa ccggttcaat
     7261 tcccgcggat tgtgggtgtt tcggagaggg cagttttgtc cattttcccg cttaccagat
     7321 ttttattctt gatgtcatca gcacgcttat tgggatccgt ctttgtttga tgcgttcgca
     7381 tcccttaagc ctggatgccc tgttgggcaa gtaatctgtt ttttgaaaga aactttgaat
     7441 tttgtttaaa gattttgaca ttcgtgaacg cttccttata attcacccca tcgattgaca
     7501 gtccgacgag gtttttaact cgttgggtac atgggcattc tcgggcaggt agctcagtcg
     7561 gtagagcaag ggactgaaaa tccctgtgtc gggggttcga ttccctccct gcccaaatct
     7621 tcttaaaaag cagactaccg cataaagcgc gatagtttgc ttttttgttt tagctcgatg
     7681 aaaaatccgg atagtcccga ccggttgcct ttttattttc aaactggatt gaaacggtct
     7741 ggtttgataa aatcaccctg ccacgaaaag gaccatccat gcgatatacc accaaaactg
     7801 aatatggagt gatttgtctc gcctacatgg cgatgccggg gcggactgtg ggcagtaccg
     7861 cgaaagagat agcggagtgc ggaggatttt cggtgccctt taccgaaaaa atccttcaaa
     7921 aacttcgtaa aacggggatg gtttcgtcac aacagggaaa tcgcggaggg tggactctcg
     7981 cgcgggctcc acgagagatc agtttgaagg agattgtaga ggctcttgag ggaagcacgt
     8041 ttgaaacttt ttgcgattcg aaacgacgcg gaagtatgga gtgtagacat ttttctgttt
     8101 gtaaactgaa acccatctgg tacagaacca aagacaccct ggacaatctc tacgagaaaa
     8161 tcaccttgga tcaaattgcc agagacctga tggggagcaa gtcttccagg tttcttgaga
     8221 ggaaaaggaa agaacggtca tgacggacga aggggctttt cttgataagg agtatccgta
     8281 cggatttgtc acgccggtcg agtcggagac tgctccccgc ggtctgaacg aggatattat
     8341 ccggttcatt tccaagaaaa aaaatgaacc ggaatttatg ttgcggtggc ggcttaaggc
     8401 gtttcgggag tggcaaaaaa agaaagagcc gtcctgggcc agccttcact aacct
//
LOCUS       DAOSIO010000056         8442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_7849_length_8442_cov_4.238822, whole genome shotgun sequence.
ACCESSION   DAOSIO010000056 DAOSIO010000000
VERSION     DAOSIO010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7849_length_8442_cov_4.238822"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PK590_03275"
     CDS             <1..389
                     /locus_tag="PK590_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017943398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOG23650.1"
                     /translation="IVVAIDAKREGKNRWGVYTHGGRQSAGLDAIEWAKKAEKLGAGE
                     ILLTSMDCDGTQKGYDLDLTRRISNSLRIPVIASGGVGNLEHLYQGFSRGKADACLAA
                     SIFHFGDYTISEAKIFLKKKGILVRL"
     gene            386..1009
                     /locus_tag="PK590_03280"
     CDS             386..1009
                     /locus_tag="PK590_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23651.1"
                     /translation="MNSFLSARVFSAMPRFDTIAYQGAVVPKLLKFTVGREQSLRLLF
                     LSSVVSAEEKVAWKSFQDLLGYSGTLAQSNLSGLFGLDVLSVNIREGIRHFRAQEYAS
                     LLVNHSRNLGSEHKALVRYGQIFAVLHKRAPADWGKIDIQTHVEIIHPDKTGQASHIR
                     KIRRAAGNPSDAIYLIHDLSSAPLWKQEDPTAEVPPEVYIHAEPLLE"
     gene            1210..1605
                     /gene="hisI"
                     /locus_tag="PK590_03285"
     CDS             1210..1605
                     /gene="hisI"
                     /locus_tag="PK590_03285"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOG23652.1"
                     /translation="MERLKFNEQGLLPAIIQDSETGEVLMLAYMDKIAVERTLKEKRT
                     VFYSRSRNKYWVKGEQSGHTQDVVQTLTDCDKDTLLIRVMQKQGGACHLGYRSCFVHE
                     MNEQGDIVRITQKKVFDPDQVYPGKQGQV"
     gene            1608..3083
                     /gene="trpE"
                     /locus_tag="PK590_03290"
     CDS             1608..3083
                     /gene="trpE"
                     /locus_tag="PK590_03290"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOG23653.1"
                     /translation="MITPDFQTFRQLARRGNLIALTETFPADLETPVSSFLKISRKSR
                     QAFLLESAEQGEHVGRYSILGIDPIAVYSMKKDKVTIEEPLGTRRVIRSAHLLDLIDK
                     ELSTIRIANPRDFHGFSGGFVGYFSYENVSRFEKIQLTPKKGAGFPEGIFFRVQDFLV
                     FDHYRKTLSIVILVDIRKEKNLARAYKNAHVRARKYQNLLRRPLKIPPTTDDTPPPKL
                     TLHISRAEYEAKVRRIKEFIRAGDCIQVVLSQRFSLPHRGHDFRIYRSLRSINPSPYM
                     FYFRSGPYRLIGSSPEVLVKKTGRTACVRPIAGTRPRGKTEREDLVLEKQLRNSPKEM
                     AEHLMLVDLGRNDLGRVSDFKSVRVENFAYVERYSHVMHLVSNVLSRLKKGKTAADLL
                     KATFPAGTVSGAPKIRAMQIIDELEPESRGPYAGCLGYLGFDGNMDMCITIRTLMLER
                     KKMFIHAGGGIVNDSNPAREYEETVNKSKAVLKAIEERTLF"
     gene            3083..3655
                     /locus_tag="PK590_03295"
     CDS             3083..3655
                     /locus_tag="PK590_03295"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOG23654.1"
                     /translation="MLLVIDNYDSFTYNLVQYLGELDADPRVYRNDEITISKIRRLRP
                     RQILVSPGPCTPREAGISNDVIRTFSGLIPILGVCLGHQCIGHVFGAKIVRAKNIMHG
                     KTSKIYHAKDPLFSGMSNPFVATRYHSLLVEKLTIPDTLKITAWTREKEIMGLRHRTH
                     PTVGVQFHPESIMTKEGMRLLKNFLSLSKQ"
     gene            3652..4704
                     /gene="trpD"
                     /locus_tag="PK590_03300"
     CDS             3652..4704
                     /gene="trpD"
                     /locus_tag="PK590_03300"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOG23655.1"
                     /translation="MKKIRQSIYHKFDRMLLRLLAGKSLSRLESRKAFTLLFSEKISF
                     EKAKSFLLLLSRKGETAPELLGCVQALQFLEKISRDNVPGLMDTCGTGGDGRHTINIS
                     TLVALVIAGAGGKIAKHGNRAISSRSGSSDLMEAFGVKLAASRKKMIRAAQHSGLGYF
                     HAPYHHPVFSKMQPLRKALKRPTILNLLGPLVNPLRLDYQLVGVSQKRLLPLYAEVLS
                     KLGRKNALVCHSQDGMDEISTAAPTHAAWVTPNKVRMAVIRPNALGLKTARSKDLVVS
                     SVQENKWRAKNILTGKDQGPARDTIVLNAAYGLLICQKVSTVQEGVVLAQKSIDTGSA
                     IRVLQHLKKWSTSHGS"
     gene            4694..5494
                     /gene="trpC"
                     /locus_tag="PK590_03305"
     CDS             4694..5494
                     /gene="trpC"
                     /locus_tag="PK590_03305"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013326555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOG23656.1"
                     /translation="MVLNDIVIQRKKDVTELKQRMPLSRLQRIIETMPRPPARSFIKA
                     ITASKNLNVIAELKKASPSHGVIREDFQPLRIANLYEYAGACAISVLTEPHFFKGRPS
                     YLKTVRQVTTLPLLRKDFILEKYQLYETAILEADAFLLIAAILTEEELKELIDLGRSL
                     GMDALVEVHNNSDLKKAVNAGAKMIGINNRNLKTLEIDPLLATQFIPKIPKGTAIVVE
                     SGLGTHEELMMYKSLGANAFLVGTSLIKSPDIALALQQLLGKQATGSL"
     gene            5500..6126
                     /locus_tag="PK590_03310"
     CDS             5500..6126
                     /locus_tag="PK590_03310"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOG23657.1"
                     /translation="MTRIKICGNTNLENARLAVDLGADYLGFIFAPASKRAITESQAR
                     DIIDHLDGFQNTVGVFVNQPKKDVEKIAGTLGLKYLQFHGDETALYCEHFMKEGYEVI
                     KTFHIKDAMSLKRIDEYNVSAFLFDTFSPSERGGGGKSFDWSVIEDRPYVHEKLFLAG
                     GLSASNVADAIRTVEPFAVDVASGVEISPGIKDVQLLKKFISAVRKVK"
     gene            6126..7313
                     /locus_tag="PK590_03315"
     CDS             6126..7313
                     /locus_tag="PK590_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG23658.1"
                     /translation="MEKLDFLKKIRSRLSNVEKETLYQKLREEVQDSLLFRHVCQSLE
                     EGILLLDLRGHLTYVNPRAETWLGISPGKKSAAPVWTILEDRQLSAFLEKNISEIRQK
                     TVSNLKIITPREMEFRVSLEPYAIGDREGFLLILRDTTHRSEREFEDAMLQRMDSLIR
                     LAGGIAHEIGNPLNAITIHLELLKKRLKGIPEAKRTELETYLASIQQETKRLDKIIRN
                     FLKATRKPPLRFQTNSLDEILTDTLNFLKPELTASKIRLKFTHDDTIPPFLLDRERLY
                     YAFMNLVKNAMESMPRGGDLKIALIRKGNCAIVSIQDTGCGIPKEDLQHIFDIYYTTK
                     EEGSGLGLMMVYDAITEHGGRIEVTSKLKKGTTFRISLPIREPRKQLPDYATSNGGYV
                     SHA"
     gene            7306..>8442
                     /locus_tag="PK590_03320"
     CDS             7306..>8442
                     /locus_tag="PK590_03320"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG23659.1"
                     /translation="MLESILLVEDDSNSRKGLTQFLQALDYDVMTAVNGKEGYDIFKE
                     ENPDLVIADIRMPEMDGVTLLEKIRDEKPSAKVILLTAYGSVEDAVKAMKKGAFYYLT
                     KPVNLEELEFLVKKAFSSHQLEEENRELRRELFSERFHEGILIAESLKMKELLKTVDK
                     IAASNASVLIEGESGTGKELIARRIHEMSSRSQQPFVAVHCAALTETLLASELFGHEK
                     GAFTGAIDRKKGRFERAHQGSLFLDEIGEITRETQVKLLRVLQEGEFERVGGTKTIKV
                     DVRLIAATNKTLIEEVKKQNFREDLYYRINVIYLKVPPLRERKEDIPPLVQAFLRQYG
                     TQNSRKIGVIDKEAMAALTRYDWPGNVRELKNVIERMTILSTGNR"
BASE COUNT         2263 a         2026 c         2083 g         2070 t
ORIGIN      
        1 gtattgtggt cgcgattgac gcgaaacggg aagggaaaaa ccgttggggg gtttacacgc
       61 atggtggccg ccaatcggcc ggactggacg ccatcgaatg ggcaaaaaag gcggagaaac
      121 ttggcgctgg ggaaattctt cttaccagta tggattgtga tggtacccag aaaggctatg
      181 accttgattt gacacgccgg atatcaaact ccctccgaat cccggtgatc gcctccggag
      241 gcgtcggaaa cttggaacat ctctatcaag gattttctcg tggcaaggct gatgcctgtc
      301 ttgccgcctc tatttttcac ttcggtgact acacgatttc cgaggctaaa atatttttga
      361 aaaagaaagg tatcctcgtc cggttgtgaa ttcgtttctt agcgcccgtg ttttttctgc
      421 gatgccgcga ttcgatacca tcgcgtatca gggtgcggtc gttcccaagc ttctgaaatt
      481 taccgtcggt cgggaacaat cactccggct tttgttcctt tcatccgtcg tatccgcgga
      541 ggaaaaagtc gcctggaaat cttttcagga tttgcttggt tattcgggca cccttgccca
      601 aagcaatctt tcaggattgt ttggtctcga tgtcctcagt gttaacatcc gagaaggaat
      661 ccggcatttc agagcgcaag aatacgcctc acttttagtc aaccattccc gaaacctcgg
      721 atctgaacat aaagcgcttg ttcgttacgg acaaattttt gccgtgctcc acaaacgggc
      781 gcccgctgat tggggaaaaa tagatattca gacgcatgtc gagattattc atcctgataa
      841 aactggacag gctagccata tcagaaaaat tcgtcgtgcc gccggcaatc cctctgatgc
      901 gatctactta attcatgatc tcagttcggc ccccctctgg aaacaggaag acccgacagc
      961 cgaagttcct ccggaggttt atatccatgc agaacctctt ttggaataag gcaaccttat
     1021 gccaacaaag actggttgta ataggctttc agttatgttt ttctgtgaat agtctaacag
     1081 ttggatacac ccgaaaagtg tatccacttg acacatttta agaatagagg catactcacg
     1141 ctcatacttc tgattgatta tcacgcctga gattgacgta actctattgt tcaagggaga
     1201 atacgaaaaa tggaaagatt gaaatttaat gagcaagggc ttcttcccgc tattatccag
     1261 gattcggaaa caggggaagt cctgatgttg gcttacatgg ataaaatcgc ggttgaacga
     1321 actcttaagg aaaagcgtac cgttttttac tcgcgttccc gtaataaata ctgggttaag
     1381 ggagagcagt ccggccacac ccaggatgtt gtccaaacgc tcacggattg cgataaggac
     1441 acgctactga tccgcgtaat gcaaaaacag ggcggcgcgt gtcacctcgg ctaccggagt
     1501 tgctttgttc acgaaatgaa tgagcaaggg gatattgtcc gaattacgca gaagaaagtt
     1561 tttgatcccg atcaagtcta tccggggaaa caaggccaag tctaaaaatg atcacgcccg
     1621 attttcagac attccgccag ttggcgcgcc ggggaaatct gatcgccctt accgaaacct
     1681 ttcccgcgga tttggaaact ccggtttcca gctttctcaa gatctccaga aaatccaggc
     1741 aggcctttct cctcgaatcg gctgaacaag gggaacatgt cgggcgatac tcaattctcg
     1801 gaatcgatcc gatcgctgtt tacagtatga agaaagacaa ggtcacgatc gaggagcctt
     1861 tgggaacccg acgggtcatt cgttcggcac acctcctcga tctcattgac aaggaattga
     1921 gcacgatccg tatcgcaaat cctcgggact ttcacggatt tagcggcggt ttcgtcggtt
     1981 atttttctta tgaaaatgtc agccgttttg aaaaaataca attaactccc aaaaaaggcg
     2041 cgggatttcc ggaagggatt tttttcaggg tccaggattt cctcgtgttt gatcattacc
     2101 gaaaaaccct gtcgattgtc attctggtgg atattcgtaa agaaaaaaat cttgcgcggg
     2161 cttacaaaaa cgctcatgtg cgcgcgcgaa aatatcagaa tttgctacgt cgaccgctga
     2221 agattccacc aacgactgat gacactccgc ctcccaaatt gacgctgcac atttcgcgag
     2281 ctgaatacga agcgaaagtt cgccgaatta aggaattcat ccgtgctggg gactgtatcc
     2341 aggtcgtttt gtcccagcgg ttttcgttgc cacatcgggg acatgatttt cggatctacc
     2401 gctccctccg ctccattaat ccctctccgt atatgtttta tttccgaagc ggtccgtatc
     2461 gtttgatcgg ttcctcaccg gaggttctgg taaaaaaaac aggtcgtacc gcgtgtgtcc
     2521 gcccgatcgc ggggacacga ccgcggggaa aaacggaacg agaagatctc gtattggaaa
     2581 agcagctcag gaattcgccc aaagagatgg cggaacactt gatgctggtc gatctcggtc
     2641 gtaacgatct tggccgggtt tcagacttca agtccgtccg ggtcgaaaac ttcgcttacg
     2701 tcgaaagata ttctcacgta atgcaccttg tcagtaatgt tctcagtcga ttaaaaaaag
     2761 ggaaaacggc cgcggacctt ttgaaagcca cgtttcccgc cggaaccgta tcgggggctc
     2821 cgaaaatccg tgccatgcaa atcattgatg agcttgaacc ggagtcccgg ggaccctacg
     2881 cggggtgtct cggctatttg ggttttgacg gaaatatgga catgtgtatt acgatccgga
     2941 ctctcatgct cgaaaggaaa aaaatgttca ttcacgcggg gggcggtatc gtcaatgatt
     3001 ctaaccccgc gcgtgaatac gaggagacag tcaacaaatc gaaagccgtg ttgaaggcga
     3061 tcgaggaaag gactctcttt taatgttgct ggtcatcgat aattacgact cattcactta
     3121 caatctcgtt caatatctgg gggagctgga tgccgatccc cgcgtgtatc gaaatgacga
     3181 aattacgatt tccaaaattc gccggcttcg cccgcggcag attctcgttt cgccgggacc
     3241 gtgtaccccc agggaagccg ggatttccaa tgacgtgatt cgtactttca gcggattgat
     3301 cccgatcctt ggcgtctgtc ttggacacca gtgtatcggg cacgtttttg gggcaaaaat
     3361 tgttcgtgcg aaaaacatca tgcacggcaa aacctcaaag atttatcacg ccaaagatcc
     3421 gcttttttct ggaatgtcca atccctttgt tgcaacacgg taccactcac tcctggtcga
     3481 aaagctcacg atcccggaca cgctcaaaat cacggcctgg actcgggaaa aagagatcat
     3541 gggattgcgc cacaggactc atccaactgt gggagttcaa tttcatcccg aatcgattat
     3601 gacaaaggaa gggatgcgtc ttttgaaaaa ttttctgagt ctttccaaac aatgaagaag
     3661 attcgccaat ccatctacca taagtttgac cgaatgttac ttcgcctcct ggcagggaag
     3721 tcgttgtccc gtcttgaatc caggaaagcg tttacgttgc ttttttcgga aaaaatttca
     3781 ttcgaaaagg caaaaagttt tttgttgctg ctcagccgaa aaggggagac cgcccccgaa
     3841 ctgcttggct gtgtccaggc gcttcaattt cttgaaaaaa tttcccgcga caatgttccc
     3901 ggtttgatgg atacctgcgg cacaggggga gatggtcgtc acacgatcaa tatttccacg
     3961 ttggtcgcgc tggtgattgc tggggcgggc ggtaaaatcg ccaaacatgg taatcgagcc
     4021 atttcatcca gatccggaag ttctgatctc atggaggcct ttggcgtgaa attggccgcc
     4081 tcgcgaaaaa aaatgatccg tgccgcccaa cattccggcc tgggttattt tcatgcgcca
     4141 taccatcatc ctgttttttc aaaaatgcaa ccgcttcgga aggcattgaa acggccaacc
     4201 attctcaatt tacttggtcc gcttgtgaac cccctccgac tggattatca gcttgtggga
     4261 gtgagccaaa aacgtttgct tccactttat gccgaggtcc tttcgaaatt gggccgcaaa
     4321 aacgccctcg tctgccatag tcaggatggt atggatgaaa tttcaacggc ggctccgaca
     4381 catgccgcct gggtcacacc gaacaaagtt cgtatggccg ttatacggcc caatgcgctg
     4441 ggtcttaaaa cggctcgttc gaaagatctt gtggtgagct cggtccagga aaacaagtgg
     4501 cgcgccaaaa atattttgac cggtaaagat caagggccgg cgcgagatac catcgtattg
     4561 aatgccgcct acggtctttt gatttgtcaa aaagtctcga cggttcagga gggtgtcgtc
     4621 cttgcccaaa aatccatcga taccggaagc gcgattcggg ttcttcagca tctcaagaaa
     4681 tggtcaactt cccatggttc ttaatgacat tgtgatacaa agaaaaaagg acgttacgga
     4741 attaaaacag agaatgccgc tgtcccggct ccagcgaatc attgaaacca tgccacggcc
     4801 tccggcccgg tcgttcatta aggccatcac ggcttccaaa aatctgaacg tcatcgctga
     4861 actcaaaaag gcatctccct cacacggtgt gatccgcgaa gattttcaac cgttacgaat
     4921 tgccaatctt tatgaatacg ctggcgcgtg cgcgatctcc gttctcaccg aaccgcattt
     4981 ctttaaagga cgtcccagtt atttaaaaac tgtacgccag gtcacgacgc taccgcttct
     5041 ccgaaaagat tttattcttg aaaaatacca gctttatgag actgcaatcc tcgaggcgga
     5101 cgcctttttg ttaatcgccg cgatccttac ggaagaagaa cttaaagagt tgattgacct
     5161 gggccgatcc ctcggaatgg acgccctagt cgaggtgcac aacaattcag atctgaagaa
     5221 ggccgtaaac gcgggcgcaa aaatgatcgg cattaataat cgaaacctca agacgcttga
     5281 aattgatccg cttcttgcga ctcaattcat tcctaaaatt cccaagggga cggccattgt
     5341 agtcgaaagc ggacttggca cgcatgagga actcatgatg tataaaagtc ttggagcaaa
     5401 tgcctttctt gtggggacaa gcctcataaa gtctcccgat attgccctgg cgcttcaaca
     5461 acttctcgga aaacaggcga ccgggagcct ttgatcctta tgacacgaat taaaatttgc
     5521 ggaaatacca atcttgaaaa tgcccgactt gcggttgatc ttggagcgga ttatctgggt
     5581 ttcatctttg ctcccgcgag caagcgggcg atcacggaaa gtcaggcgcg cgatatcatc
     5641 gaccacctgg atggattcca gaacacggtc ggtgttttcg tcaatcagcc caaaaaagac
     5701 gtcgaaaaaa tcgcggggac tctcgggctg aaatatctcc agttccatgg agatgaaacc
     5761 gcgctttatt gcgaacattt tatgaaggaa ggctatgaag tcatcaaaac ctttcacatc
     5821 aaggacgcca tgagtctaaa acggatcgac gaatacaatg tcagcgcgtt cctttttgac
     5881 acgttttcac cctcggaaag aggcgggggt ggaaaatcct ttgactggag cgtgatagaa
     5941 gatcgtcctt atgtgcatga aaaattattt cttgccggcg gcttgagcgc ctcaaacgtg
     6001 gctgatgcca tccggaccgt cgaacctttc gcggtggacg tggcaagcgg agtggaaata
     6061 tcgccgggaa ttaaggatgt tcagctttta aaaaagttta tctcagcggt tcgaaaggta
     6121 aagtaatgga aaaactggac tttttaaaaa aaattcgttc gcgtctttca aacgtggaaa
     6181 aagaaaccct ttatcaaaaa ctccgggagg aggttcaaga cagcctgctc tttcggcacg
     6241 tttgtcaaag tctggaagag gggattttgt tgctggactt gcgaggacac cttacttacg
     6301 tgaacccgcg cgctgaaacc tggctcggga tctctcccgg caagaaaagt gccgcgccgg
     6361 tctggaccat tcttgaagat cgtcagctat cggcttttct ggaaaaaaac atttcggaaa
     6421 tcagacaaaa aactgtcagt aaccttaaaa ttatcactcc tcgggaaatg gaatttcggg
     6481 tgtccctgga gccgtacgcg ataggggaca gggaaggttt tcttctcatc ctgagggaca
     6541 cgacgcatcg ctccgaacgg gaatttgaag acgcgatgct ccagcgcatg gacagtctga
     6601 tccgtctggc cggtggtatt gctcatgaga tcggcaatcc cctgaacgcg atcaccattc
     6661 accttgaact tctcaaaaaa cgtctgaagg gtattccgga agcaaagcga acagaactgg
     6721 aaacttatct ggccagcatt caacaagaaa caaaacgact cgacaaaatt atccggaatt
     6781 ttctcaaagc gacgcgcaaa cctccgctcc ggtttcaaac gaattctctg gatgaaattc
     6841 ttacggatac tctgaatttt ctcaagccgg agctgacggc ctccaagata cggctcaaat
     6901 tcacgcatga tgacacgatc cctccttttc tgttagatcg ggagcgactt tactacgcgt
     6961 ttatgaatct ggtcaaaaat gcgatggaat ccatgccgag gggaggggac ttgaagatcg
     7021 ctctcattcg taagggaaac tgcgcgatcg tatcgatcca ggataccggc tgtggaatcc
     7081 cgaaggaaga tctccagcat atttttgaca tctattatac gacgaaggaa gaaggatcag
     7141 gactgggact catgatggtt tacgacgcga tcactgagca cggaggacgc atcgaagtaa
     7201 cgagcaagct gaaaaaagga accacattca ggatttctct tccaattcgg gaaccccgaa
     7261 aacaactccc tgactacgcg acatctaacg gaggctacgt ttctcatgct tgaaagcatt
     7321 ctactggtcg aagatgattc aaactccagg aaaggactca cccaattttt gcaagccctg
     7381 gactatgacg tgatgaccgc cgtcaacggg aaagaaggat acgatatctt caaagaggaa
     7441 aatcccgatc ttgtgatcgc cgatatacgg atgcctgaaa tggatggagt cactttgctt
     7501 gagaaaatcc gggacgaaaa accttcggcc aaggttattc tcctaacggc ctacgggtcg
     7561 gtcgaagacg ctgttaaagc tatgaaaaag ggcgcttttt actatttgac gaaaccggtc
     7621 aatctggaag agcttgaatt tctcgtcaaa aaagccttta gcagtcatca gttagaagag
     7681 gaaaacaggg aactgcggcg agaacttttc agtgaacgat tccacgaggg aatattaatt
     7741 gccgagtcgc taaagatgaa agagctcctt aaaaccgtcg ataaaattgc cgcttcgaac
     7801 gcttcagtgc ttattgaagg tgaaagcggc acgggaaagg agctgatcgc gcgtcggatc
     7861 catgaaatga gttcccgttc ccaacagcca tttgtggcgg ttcattgcgc ggcgctcacg
     7921 gaaacgcttc ttgccagtga gctttttggc catgaaaaag gcgcatttac gggcgcgatc
     7981 gaccggaaaa aaggccggtt tgaacgcgcg catcagggga gtctttttct cgatgaaatc
     8041 ggggaaatta cgcgagagac ccaggttaaa cttcttcgag tcttgcagga aggggaattt
     8101 gaacgcgtgg ggggcaccaa aaccatcaag gtcgatgttc ggctgatcgc ggccaccaat
     8161 aaaaccctga ttgaggaagt gaaaaagcag aattttcgcg aggatctgta ttaccggatt
     8221 aacgtaattt atcttaaagt gcctccgctt cgtgaaagga aagaggatat tcctccattg
     8281 gtccaggcat ttcttcgtca atacgggacg caaaattcaa gaaaaatagg cgtcattgac
     8341 aaagaagcca tggcggcgct tacccgctac gactggccgg ggaatgtccg ggaacttaaa
     8401 aatgtcatcg aacgcatgac tattcttagc acggggaacc gc
//
LOCUS       DAOSIO010000057         8332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_7997_length_8332_cov_4.289960, whole genome shotgun sequence.
ACCESSION   DAOSIO010000057 DAOSIO010000000
VERSION     DAOSIO010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7997_length_8332_cov_4.289960"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..1009
                     /gene="dapF"
                     /locus_tag="PK590_03325"
     CDS             197..1009
                     /gene="dapF"
                     /locus_tag="PK590_03325"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOG23660.1"
                     /translation="MPNSIPFYKMVASGNDFVVVDNRRKAVRNVSAFTREICALHQGA
                     GADGVLLFEGSSRADFKMRIINSDGSEAAACGNGFRCIALYAREIMKRPPAMKFESGS
                     GLIEANVRTGGVVRVQLVKPKIFEMNGTVEVLGRQIHYSFLDTGVPHAVVFVEGISKL
                     DVVGIGRAMRYHKRFKPAGTNVNFVEIKNAKEIMVRTYERGVENETLACGTGSTASAV
                     ISVLKGYVKAPVTARTRGGELLTIDMELKNGEIQKTFLQGKARFVYKGEYFL"
     gene            1006..1896
                     /gene="dapA"
                     /locus_tag="PK590_03330"
     CDS             1006..1896
                     /gene="dapA"
                     /locus_tag="PK590_03330"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOG23661.1"
                     /translation="MSKKNPDLKGSMVALVTPFRNGKVDRKAMKGLIDFQIKSGTDVL
                     VPCGTTGESATLSHEEHRDTLSFVVDYVDGRVPVIAGCGSNNTAEALGLVRHARKIGA
                     SAALIVTPYYNRPTQEGLYRHYEFLASRVDIPIVLYNVPGRTGVSIAPETVARLSKID
                     TIVAIKEASGSMDQADKIMQLCDIAVISGEDSLTYPLIAIGAQGVISVTANVVPGLVH
                     DMVWAALDGKMTQAAELHRRHYPFSKVAFIETNPIPVKTALGWMGKLKPELRLPLCEM
                     RPENEKKLLEVLKQLGIPGK"
     gene            1912..2715
                     /gene="dapB"
                     /locus_tag="PK590_03335"
     CDS             1912..2715
                     /gene="dapB"
                     /locus_tag="PK590_03335"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOG23662.1"
                     /translation="MVKLAVAGAAGRMGKMILQLAARDKAFKITGGIEQRGHATIGMD
                     IGPVIGCDLLNAKVEQDPFPVVKRADVLIDFTHFSALPENLKACVAANTAIVIGTTAI
                     SPVLMKKIVSASKKIPVLQSPNMSVGVNLLFNLAAMAGSSLDSSYDLEITEVHHRMKK
                     DAPSGTALKLLEILADSRGVDSRKDVVYGRQGETGSRQQGKIGVFALRGGDVVGEHTV
                     SFLGDGERVELVHKASSREPFAKGALLAAKFLAKKRAGLYHMRQVLGMP"
     gene            2784..4361
                     /locus_tag="PK590_03340"
     CDS             2784..4361
                     /locus_tag="PK590_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23663.1"
                     /translation="MKLKNIITSFLACVIVLCHSSPVATASILIPEADSAEATPKPNL
                     FAQNFYMPSGQKLDQKITEIRGFPIVKTHSRPQNSEYSIVTDFGDNAFRSRFLPGLKD
                     PKAIDDYIKQNLQVANFNGVEVRKLTIPLVDGQATDMFWVGDKAFPSAEQATAEVSRI
                     KSLIESQGGDFGASVREVPVFVAPEQPETVKTQAQFKKEEELILKFTDQLDIGEKMWG
                     PFQGEGAGEPLLWQSFGETTYRMTNLENRNFNSQVGYWSNRLVFKGFRFPLNTIEPFV
                     EETTSIEAVGADYKSHFDLFAGLQWYPLLRNPWLYNFRPFGWDIPVLEWIRNYKFYIK
                     YGNRYNIKDQIENAKDYDLIAGVQIFYEWGTDLPSLAEGKPERFADYLRQYVWGEYFG
                     DYRWEKTNFGSEDDFNAYILNSSVIMGIKLPGIPLPHNPINDEIVLMPYLRFEHVNNC
                     EFSFPYQNRYFLAPGIRWMPFRAYRWKEDEWLSKLAIFGEWLAIGKVQNAKQDGEAPE
                     AVRYDLRFGVKFSHRRF"
     gene            4382..>8332
                     /locus_tag="PK590_03345"
     CDS             4382..>8332
                     /locus_tag="PK590_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23664.1"
                     /translation="MNIRSLEKQKNVWKKRTLLPLVCFLVCLVSFNVPFAFANVLGNG
                     DFSQPLGSGTDGNWDNTQGATRVQFGTPGYPAGFSAPPNGQYGLRLDGNSGAFTFQTK
                     DNVKPGALVTFSAMAMSDVDNTKAAHQMKIEFKKINPDGSDTTILTVPNPAPCTPTTC
                     VNNLSAAGGFKPFQVSAIAPEGTGRVAFVFHRLWGPGGPAIGQTVFADANAEVNPAKL
                     SVSASKRNVKKGDAIGINAHFNNASGNTFANSNMRIKLPNGFDHAQDSIRVNGQKVAG
                     QEGSLIIPLGTITPGETYDVTFATLVTSGVQLGKTYSLEVTVEGGGGALSETARVQFT
                     VQGDPIFDEGTIIGKVFNDLNQDGVQQCGEQGVPWVTLYTEEGIKIVTDEFGRYHIPG
                     VSPGRHLVKIDGHTLPEGTKFVTEETYLVKITPGIMGKANFAVLLPPSGIPETFRDDL
                     MVMITQGADISRPNLDIAMEPSVLKLGVGLLEKEPLFKFDSNYAGFAKKWTLEIRDEM
                     GTPVWKGFGVGQPPPEMTWNGKTENGLMIKPGIYSYQLKIRDSKNREDWTPLKFFEVY
                     NKLDERTKKNYQTEIPSVGDFNLFKDGKRSIPLIAKPTVRIQGKTKPDYEIKINEIPV
                     SVDPETGMFQKEFYVEPGEREFTISATSAEGESTSYQEKVQVKDSTFFMVALGEEQLG
                     VNFSKGAMEAADTDAYRDGFTEEGRFSYYLKAKLKGKFLIKSHYDTSDRRSALFTNLD
                     PDEYYPIYGDGSSRDYEAQDTAQRLFVLVEMDKSFLKWGSFKTGFTDTELASYNRTLS
                     GLQISFDTVASTVYGDPKRGFKLFWTESGKQADHNEFYATGGSLYYTRQRNIVEGSEK
                     IRVEVRDKITGMVLSSYDMTEGADYEISYGEGRILLSRPLSSVAATDTLVTADIMDGN
                     PVYLIVDYEYEPSSSDLPEISNRGIRGYTWAGDHVRVGATAVEEKRAGGQDYDLRGVD
                     MMLKFGRNTQITAEYATSINKQMDSSVSYDGGISFGTEGNLRGNNTRPRENAYVIRGQ
                     SQPVRNLEVSGYVQGVEPGFSNGHMTSQEGFKKYGIATRYKFTDTFSARYRFDSSEVI
                     GQLLPLAEHNIAAPYEGLQVHTAQLVYDDGKYLAQAEYQRSNIDHVENSANLVPSLYS
                     EVPFEHGIATKIGYHINDRLLPYVKAQTTLHSADDYQVGGGIRCELIKNVFGYIEQMF
                     GPLGDSTHYGFEKQHDNGARSYASLRSVDRGIGSRTLSTAIGSSFPLTEKSRMYSERE
                     HTSYQGVDGFADILGYEGSLGDHWDFGAKYERRHLDSSMARAIVDVVMNGQAVNN"
BASE COUNT         2089 a         1862 c         2286 g         2095 t
ORIGIN      
        1 aacggcgcag aacgtcttct gaaattgagg cagacttatc tgaacaatca atggggcagc
       61 ctcctacagg cctcaagaaa ttaactgcct aaagtgggat acaccccatc aaccgttgat
      121 gtttcccgtt ccccgtttcc cggttctttg atacaataca ccctcttcta actagcggaa
      181 tgataaggag ttatgcgtgc cgaattcgat tcctttttac aagatggtag cctcgggtaa
      241 tgattttgtg gtggtcgaca atcgacgcaa ggcggtacgg aatgtgtccg cttttacgcg
      301 tgagatttgc gcgctgcatc agggggctgg cgctgatggc gtactgcttt ttgaaggttc
      361 ctcccgggcg gatttcaaaa tgagaattat caattctgac gggtccgagg cggcggcgtg
      421 tgggaacgga tttcgctgta ttgcccttta cgcgcgggag atcatgaaga gacctccggc
      481 catgaaattt gaatccggct ccgggttgat tgaagctaat gtcagaacgg gcggcgtggt
      541 gagggttcaa ctggtcaaac cgaagatttt tgaaatgaat ggaacggtgg aggtcctcgg
      601 ccgtcaaatc cattattctt ttcttgatac cggagtgccg catgcggtag tttttgtcga
      661 gggaatctcc aaactagacg tggtgggtat cgggcgtgcc atgcggtatc acaaacggtt
      721 caagcccgcg ggtacgaacg tcaattttgt tgaaataaaa aacgcgaaag aaataatggt
      781 tcgaacctat gaacgtggag tggaaaacga gactcttgcc tgtggtaccg ggtcgaccgc
      841 ctcggcggtt ataagtgttc tgaaaggcta tgtgaaggcg cccgttacgg cgcggacgcg
      901 gggcggcgaa ctcctcacga ttgacatgga attaaaaaac ggcgagatcc agaaaacttt
      961 tttacaaggc aaggccagat tcgtttataa aggagaatac tttttatgag caagaaaaac
     1021 cccgacttaa aaggttcaat ggtcgcttta gtcaccccgt ttcggaacgg caaggttgac
     1081 cggaaggcga tgaagggtct gattgatttt cagatcaagt caggaacgga tgttctcgtg
     1141 ccttgtggta cgaccgggga gtcggccacg ctgtcacacg aggagcatcg tgatacgttg
     1201 agttttgtgg tcgattacgt ggatggtcgc gtgcctgtga ttgccgggtg tgggtccaac
     1261 aatacggctg aggccttggg acttgtccgc catgccagaa agatcggagc ctcggccgcg
     1321 ctgattgtca caccttacta taacaggccc acccaggaag ggctttatcg gcattacgaa
     1381 tttcttgctt ctcgggtgga cattccgatc gtcctttata atgttcccgg gcgaacgggt
     1441 gtgtctatcg caccggaaac tgtcgcgcgt ctttcaaaaa ttgacacaat tgtggcgatt
     1501 aaagaggcga gcggaagcat ggatcaggcc gataagatca tgcaactttg tgacatagca
     1561 gttatttcgg gggaagattc cctaacgtat ccactgatag ccattggcgc ccaaggcgtc
     1621 atttccgtga cggcgaacgt tgtccctgga cttgtccatg acatggtttg ggccgctctc
     1681 gacggaaaga tgacgcaggc ggcggagctt cacagaaggc attacccgtt ttcgaaagtt
     1741 gcctttattg aaacgaatcc gatccctgtc aaaacagcgc ttggatggat ggggaagctg
     1801 aaaccggaac tccgcttgcc gctttgtgaa atgcgaccgg agaacgagaa aaaactttta
     1861 gaggttctca agcaacttgg gattcccgga aaatagagga gattaaaacc catggttaaa
     1921 cttgcggtgg ctggagcggc tggaagaatg ggaaagatga ttcttcaact ggcggcgaga
     1981 gacaaggcgt tcaagattac gggcggtatc gagcagcggg gacacgcgac gattggaatg
     2041 gacatcggcc ccgtcatagg gtgcgatctt ttgaatgcca aggttgagca agaccctttt
     2101 cccgtggtaa agcgggctga cgttctgatc gatttcactc atttttctgc cctgccggaa
     2161 aaccttaagg cctgcgtggc ggcaaataca gccatcgtaa ttggcaccac cgccatttct
     2221 cccgttctca tgaaaaagat tgtctctgcc tctaaaaaaa ttcctgttct tcaatctccg
     2281 aacatgagcg ttggggtcaa ccttcttttc aacctggcgg cgatggccgg ctccagtctt
     2341 gattcaagct atgatcttga aattaccgag gttcaccaca ggatgaagaa ggatgctccc
     2401 agcggcacgg cgcttaagct cctggagatt ctggccgatt ccagaggtgt ggacagccgg
     2461 aaggatgtgg tgtacggaag gcagggagaa acggggtccc ggcagcaggg aaaaatcggt
     2521 gtttttgccc ttcggggtgg agatgtcgtg ggggaacata cggtgtcttt tttgggagat
     2581 ggggaaagag ttgagcttgt ccataaggcg tcatctcgcg aaccttttgc gaaaggcgct
     2641 cttctggctg cgaaattcct tgcgaaaaag cgggcaggtc tttatcatat gcgccaagtt
     2701 ctcgggatgc cgtaaattgc cttgcggttt tgtttatcaa aaaaggtttg taaaagccag
     2761 aaatcgaaag ctaaaaattt ttaatgaaac ttaaaaatat aataacctcc tttttggcgt
     2821 gtgtgattgt cctgtgtcac tcttccccag ttgctaccgc cagcatcctt atccctgagg
     2881 ctgattccgc cgaagctact cccaagccta atttatttgc ccagaatttc tacatgccct
     2941 cgggtcaaaa acttgatcag aagatcactg aaatcagggg gttcccgatc gttaagacgc
     3001 attcccgccc tcagaattcg gagtactcaa tcgtgacgga tttcggagac aatgcctttc
     3061 ggtcccgctt tctccccggg ttaaaagatc ccaaggcgat tgacgattac attaaacaaa
     3121 atctccaggt cgcgaatttc aatggtgttg aggtccggaa gcttacgatc cctcttgtgg
     3181 atggacaagc gacggacatg ttctgggtag gagacaaggc tttcccttcg gcagaacagg
     3241 cgacagccga ggtttccagg atcaagtctt taattgaatc acaagggggc gattttgggg
     3301 caagcgttcg tgaagttccc gtatttgtcg cgcccgaaca accggaaacc gtcaagaccc
     3361 aggcccaatt caagaaagag gaggagttga tcctcaagtt cacggatcag cttgatattg
     3421 gagaaaaaat gtggggacct tttcagggag aaggggcggg cgagccactt ctctggcagt
     3481 cgttcgggga aacgacttat cgcatgacca atcttgaaaa caggaatttt aacagtcagg
     3541 tggggtactg gtcgaaccgt cttgtcttca aaggatttcg ttttccgtta aatacgattg
     3601 agcccttcgt ggaagaaacg acgagcattg aagcggtggg agccgattac aaaagccatt
     3661 ttgacctttt tgctggttta cagtggtacc cgcttttgag gaacccgtgg ctttataatt
     3721 ttcgcccctt tgggtgggac attccggtcc ttgagtggat ccgtaattac aaattttaca
     3781 tcaagtatgg caatcgctat aacatcaagg accagattga aaacgcgaag gactatgatc
     3841 tgatcgccgg tgttcagatt ttttatgagt ggggcacgga ccttccttcg ctcgcggaag
     3901 ggaaacccga acgtttcgcg gattatctgc ggcagtatgt ctggggagaa tacttcggag
     3961 attatcgttg ggagaaaacc aattttggat ccgaagatga ttttaacgct tatattttga
     4021 attcttcagt catcatgggc atcaagcttc ccgggatccc gctccctcat aacccgatta
     4081 acgatgaaat cgttctcatg ccttaccttc gtttcgagca cgtgaataat tgcgagtttt
     4141 catttcccta tcagaatcgg tactttctcg ccccgggaat tcgctggatg ccgttcaggg
     4201 cttatcgctg gaaagaggat gaatggcttt ccaagttggc tatttttggg gaatggctcg
     4261 cgatcggaaa agtccagaac gcgaaacagg atggcgaggc gccggaggcg gtgcgttatg
     4321 atcttcggtt cggcgttaaa ttctctcata ggcggtttta atccgagtca tgatgaattt
     4381 tatgaacatt cgatcccttg aaaaacaaaa aaacgtctgg aaaaaaagga ctctccttcc
     4441 gctcgtttgt tttctcgtct gtcttgtgag tttcaatgtt ccgttcgctt tcgcaaatgt
     4501 ccttgggaac ggcgatttca gccaacctct tggcagtgga accgatggta attgggacaa
     4561 tacgcaaggc gctacaaggg tacaattcgg aacgcccggt tacccggcag gtttttcggc
     4621 gccgccgaac ggtcaatatg gtttgcgact cgacggaaat agcggggctt ttactttcca
     4681 gacgaaggac aacgtgaaac caggtgcgct ggtcacgttt agtgctatgg ccatgtcgga
     4741 tgttgacaat accaaagccg ctcatcaaat gaagatcgaa tttaaaaaaa taaatccgga
     4801 tgggtcggac acaacgattc taactgttcc taatccggct ccttgcactc ccaccacatg
     4861 tgtgaacaac ctatctgctg cgggaggatt taagcccttt caggtgagcg ctattgcccc
     4921 ggaaggcact ggacgagtgg cgtttgtttt tcatcgcttg tgggggcccg gtggcccagc
     4981 tattgggcaa accgtatttg ctgacgcgaa cgcggaagtg aaccccgcga aactatcggt
     5041 gtcggcatcg aagcgcaacg tgaaaaaagg agatgcgatt ggaattaatg cccatttcaa
     5101 taatgcgtcg gggaatacgt ttgctaattc aaatatgcgt atcaaacttc ccaatggttt
     5161 tgatcatgcg caggattcaa tacgggtgaa cggccagaaa gtcgcgggtc aggaaggatc
     5221 cctcatcatt cctctgggga ccattactcc gggcgaaact tatgatgtta ctttcgcgac
     5281 attagtgacg agtggggtgc agttggggaa aacctattct ctggaagtta cggttgaagg
     5341 aggcggtggc gccttgtcgg aaaccgcgcg cgttcagttt acggttcagg gagatccgat
     5401 ctttgatgaa ggtaccatta ttggaaaagt ttttaacgat ttgaaccagg atggagtgca
     5461 gcagtgcggt gagcaggggg tgccgtgggt aactctttac acggaagaag gcatcaagat
     5521 tgtgacggat gaatttggcc gctaccatat tccaggtgtt tctccgggca ggcatctcgt
     5581 aaaaattgac ggacacacgc ttcccgaagg gacaaaattt gttacggaag aaacctatct
     5641 tgtcaagatt acgccgggca ttatggggaa agcgaatttt gcggttcttc tcccgccctc
     5701 gggaattcct gaaacattca gggatgacct catggtgatg attacccagg gggctgatat
     5761 ttcgcgtccc aatcttgata tcgcgatgga accttcggtt ttgaaactgg gagtcgggtt
     5821 gcttgagaaa gaacccttgt tcaaatttga cagcaattat gccggtttcg ccaagaagtg
     5881 gactcttgaa attcgtgatg agatggggac gccggtctgg aaagggtttg gtgtgggaca
     5941 gccgcctccc gaaatgacat ggaacggaaa gactgaaaat ggactcatga tcaagcccgg
     6001 gatttattcc taccagttaa agatccgcga ttccaagaat cgggaggact ggacgccgct
     6061 caaattcttt gaggtctata ataagcttga tgaaagaacg aagaaaaatt accagacgga
     6121 aatcccctcg gtaggtgatt ttaacttgtt taaagacggg aaacgcagta ttccgttgat
     6181 tgcgaaaccg accgtccgga tccagggaaa gaccaaaccc gattacgaaa tcaaaatcaa
     6241 cgaaattccg gtgagcgtgg atcccgaaac gggcatgttc cagaaagagt tttatgttga
     6301 accgggtgag cgggagttta cgatttctgc cacgagcgcg gaaggggaaa gcacgagcta
     6361 tcaggaaaaa gttcaggtta aggacagcac tttttttatg gtggcgcttg gtgaagaaca
     6421 gctcggggtc aatttttcaa agggcgccat ggaggcggcg gataccgatg cttatcgcga
     6481 cgggttcacg gaagaaggcc gtttttcgta ttacctcaag gccaagctta aggggaaatt
     6541 tctcataaaa tcccactatg atacgtcgga ccgtcgttcg gcgcttttta ccaatcttga
     6601 tccagatgaa tattatccga tttacggtga tggttcatcg cgtgactatg aggcccagga
     6661 caccgcccag agactctttg tccttgtgga gatggacaag tcctttttga aatggggaag
     6721 tttcaaaacc ggatttacgg atacggaact tgcctcttac aaccggacgc tttcgggatt
     6781 gcaaatcagt tttgacacgg tggcgagcac cgtctacggc gacccgaaac ggggatttaa
     6841 acttttttgg accgaatcgg gaaaacaggc cgatcataat gagttttatg ccaccggtgg
     6901 atctctttat tacacgcgtc agcgtaatat cgtggaaggg agcgagaaaa ttcgcgttga
     6961 ggttcgcgac aagattaccg gaatggttct ttcaagttac gatatgaccg aaggcgccga
     7021 ttatgagatc agttacgggg aaggacggat ccttctttcc aggccgctct cctctgttgc
     7081 ggcaaccgac acccttgtga cggcggatat tatggacggt aacccggttt atctcattgt
     7141 ggattacgaa tatgaaccgt cgtcttcgga tttgcctgaa atcagtaacc gcggaattcg
     7201 cggttatacc tgggcgggtg atcacgtaag ggtgggtgcg accgctgtgg aggaaaaacg
     7261 agccggtggg caggattacg atctgcgagg tgtcgacatg atgctgaaat tcggccgcaa
     7321 tacgcaaatc accgctgaat acgcgaccag tatcaacaaa cagatggatt catcggtatc
     7381 ctacgatggt ggtatcagtt ttgggaccga aggaaatttg cgtggaaata atacgcgacc
     7441 ccgggaaaat gcgtatgtta tccggggcca gtcccagcct gtgagaaacc ttgaagtgag
     7501 cggctatgtc cagggtgttg agccgggatt ttcgaatggt cacatgacga gccaggaagg
     7561 tttcaagaag tacgggattg caacacgcta taaatttacg gacacgttct ctgcccgtta
     7621 ccgttttgat tcaagtgaag tgatcggtca gcttttgccg cttgcggaac acaatatcgc
     7681 ggctccgtat gaggggcttc aggtccacac ggcacagctg gtttacgatg acggaaagta
     7741 tcttgctcaa gctgagtatc agcgcagtaa tatcgatcat gttgaaaatt ccgccaacct
     7801 ggtaccgtca ctttattcag aagttccgtt tgaacatggg atcgcgacaa aaatcggata
     7861 tcatatcaat gatcgtttgc ttccttacgt caaggcccag acgacgcttc attcggcaga
     7921 tgattatcag gttggaggcg gaatccgttg tgagctgatt aaaaatgtat ttggctatat
     7981 cgagcagatg tttggtcccc ttggcgattc aacccattat ggatttgaaa aacaacatga
     8041 taatggggcc cgtagttatg ccagcctccg ttcggtggat cgtggtatcg gctccaggac
     8101 gctctcaacc gccattggaa gctccttccc ccttaccgag aaatccagga tgtattccga
     8161 gcgtgaacac acgtcatatc agggggtgga cggttttgcg gacattctcg gatacgaagg
     8221 cagtttgggc gaccactggg attttggggc gaaatatgaa cgacgccacc ttgattcttc
     8281 catggcgcga gcgatcgttg atgtggtgat gaacggtcaa gccgtgaaca ac
//
LOCUS       DAOSIO010000058         8281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_8057_length_8281_cov_3.579626, whole genome shotgun sequence.
ACCESSION   DAOSIO010000058 DAOSIO010000000
VERSION     DAOSIO010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 8281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8281
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8057_length_8281_cov_3.579626"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PK590_03350"
     CDS             complement(<1..758)
                     /locus_tag="PK590_03350"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HOG23665.1"
                     /translation="MSNGQIVPSSYRKLLAEIVGLCDGARRTLVHAHWEIGKRIVQIE
                     QDGAARAAYGAGLLVRLSEDLTRERGVGFSPSSIKRFRQFYLLHPKGAPAHLLTWAHH
                     AELLTVRDRKTRLLLEKRADERGMTRDELRKLIRERRGIGKGDRGEVADGTPLVPKKG
                     QLGLYQIRSGADVGWPRSGALVFDHGFRFYDALDDTQARGLSAGDFVEERDGKLVKVD
                     YAKRPGYTYTAYLQEVIDGDTLWVALDAGLGGIAR"
     gene            complement(1047..2189)
                     /locus_tag="PK590_03355"
     CDS             complement(1047..2189)
                     /locus_tag="PK590_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008733030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOG23666.1"
                     /translation="MAKIGIYRQQPTGYKAFVPEPFPSSVPVEWDNDLIFLLSLADRA
                     IGKLNAIDQLVPDVDFFIFMYVRKEAALSSQIEGTQATLLDYVKAEVKLADDRTPSDV
                     DEIKNYIEAMNYGLNRLGTLPLSWRLVKEIHAKLLKGVRGEHKTPGEFRRSQSWIGGP
                     TIETAVFVPPPPHEMQEAASDLEKFFHDATKLPPLIKAGLIHAQFETIHPFLDGNGRT
                     GRLLITFYLCQEKILMRPLLYLSEFFKKNRRDYYDKLNDYRFSEKGTRDWLKFFLEGV
                     RFVSEEAVTVAKNITKLHQENVRIVSGFGRNAETALKFLNGLYRKPVVDAALIGKMTG
                     ITSKANVSALIEKFVKAGILYEITGKARYRRFLYRDYVRRFSDEKI"
     gene            complement(2617..3951)
                     /locus_tag="PK590_03360"
     CDS             complement(2617..3951)
                     /locus_tag="PK590_03360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOG23667.1"
                     /translation="MSRLVKDKVFLTVFRMAVPMLAGTFAMNMYNMVDTWFVSRLGTQ
                     ALAAMSFTFPVVMFVGFVMRGVGTGAMTVVAHALGRRKHQAAARITTHAIFLASGISL
                     ILTGAGLWTFEPLFLRLGAAGEVLELARQYMTIWYWGLIFRVLHIMLADIIIGTGDSK
                     TVSFLMVGGTLLNCVLDPVMIFGWGFFPRMGIRGAALATVIAELFVMAAAYYFVHRKY
                     HLISFLTYSRRRMMASWYRILQIGSSAMVSSVLTPVSAAIVIRIVAGFGRSAVAAVGV
                     AARIEMFAFMIPMTVGMSLVPFVAQNYGAKRFDRIQTVRRGAFVFALAFGLVSAGVLL
                     FVAKPLGRLFSSDPAVVDVLARYIYITCFGYGFLEVHRYATFCMTGIHRPMMAIILNA
                     IRVLVLMIPLAFLGAKWFGLSGVFWGRLLTDLLAAGIGVVWSGKILKKMIPE"
     gene            complement(4123..4899)
                     /locus_tag="PK590_03365"
     CDS             complement(4123..4899)
                     /locus_tag="PK590_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOG23668.1"
                     /translation="MSENTSKNPRARATAELGDPELRAILSEIPVGGITPFTTIDFPG
                     RLAGVFYLQGCSWRCRYCYNSELWPFPPGGNRISPDKILSHLKSRQGRLDGIVFSGGE
                     PTAHAGLTRWVKAVKSMGYQVALHTNGMYPDRLGEVLPLCDWVGIDIKAPFDQYANVT
                     TREGSGAVAKASLRRILSSHVPYECRTTVHPDLLKNESLLQLATELHDLGVREFHVQR
                     FRQEGCPDPELRASSQPGAVISADLQDRLKAIFPKLQVRG"
     gene            complement(4844..5032)
                     /gene="nrdD"
                     /locus_tag="PK590_03370"
     CDS             complement(4844..5032)
                     /gene="nrdD"
                     /locus_tag="PK590_03370"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOG23669.1"
                     /translation="MTLAEAKERIVLKDEERTRCEVWTRVMGYHRPVAGFNTGKKGEF
                     YERKYFKESACACDCGAR"
     gene            complement(5029..7086)
                     /locus_tag="PK590_03375"
     CDS             complement(5029..7086)
                     /locus_tag="PK590_03375"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOG23670.1"
                     /translation="MAEQFQSRKITNIRKRDGSIVPFNGVKIYDAILKAGAATGDYDG
                     TEAQFLTLAVIKVIDNRYDRGFLDIEAIQDIVEQVLITSNYLKTARAYIIYREQHKKL
                     RGDKKTVVDVISSVNEYLYKQDWRVNANANQGYSLGGMILNISGKVIANYWLSHVYPE
                     EVSNAHRHGDVHIHDLDMLSGYCAGWSLKTLLREGFNGVPDKVESGPPKHLSSAVGQM
                     VNFLGTLQNEWAGAQAFSSFDTYLAPYIRIDKLPYEKVKQSMQEFIYNLNVPSRWGTQ
                     TPFTNVTFDWVCPEDLRAEHPIVGGKEMDFTYGELGEEMAMINKAYIEVMMKGDAKGR
                     VFTFPIPTYNITPDFDWDHPNCELLFEMTAKYGLPYFQNFLNSDLKPNQIRSMCCRLQ
                     LDLRELLKRGNGLFGSAEQTGSLGVVTLNCARIGYLAKGDESALFNRVDYLLQIAKTS
                     LELKRKIIQRHIEQGLFPYTKRYLGTLRNHFSTIGLNGVNECIRNFTNDQENITTEEG
                     FKFAIKLLDHIRMRMTEFQTETGHLYNLEATPAEGATYRFAKEDIKRFPDILHAGTSE
                     RPYYTNSSQLPVAFTDDPFEALERQEQLQRRYTGGTVLHLYMGERVSSHEACKEIIKR
                     ALTNYRIPYITITPTFSICPRHGYLSGEHEFCPKCDEELIHEKMLALQGSGEPTESKE
                     ELA"
     gene            complement(7272..7985)
                     /locus_tag="PK590_03380"
     CDS             complement(7272..7985)
                     /locus_tag="PK590_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOG23671.1"
                     /translation="MSEFKKFRSFIDTIQYREKQLPSIDPRSGQFPFVTISRETGAGG
                     HNLAAKIVEFLLKEKENPLFQGWEICDHDLCERVAQDPQLKISPQALIVSEYRSAVED
                     MLTQFITGESPQEKVMRKMFSLMRDLAIFGKTILIGRGSTCLTRDLPHGIHIRLVAPL
                     SSRIALMMSAHGKDEKWAREKIAEQDKAKALLVKTFFHQDVQDPLLYDAIWNTDRVTI
                     ETIAWLIVQRIKQKAELGE"
     gene            complement(8050..>8281)
                     /locus_tag="PK590_03385"
     CDS             complement(8050..>8281)
                     /locus_tag="PK590_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOG23672.1"
                     /translation="RELSARDMVCNIRAAKLLQGIRGEPPADLAAVEDCLKRLSQLSC
                     DHPEIEEIDINPLMVYAEGKGAGVIDARIILK"
BASE COUNT         2094 a         2367 c         1948 g         1872 t
ORIGIN      
        1 cgggcaattc ctccaagacc cgcatccaac gcgacccaga gcgtgtctcc gtcgatcacc
       61 tcctgcaaat aagccgtgta ggtataaccg ggccgcttcg cgtaatccac cttcacgagc
      121 ttgccgtccc gctcctccac aaaatcaccc gccgagagac ctcgcgcctg cgtgtcgtcc
      181 agcgcatcgt aaaaacgaaa cccgtggtca aaaacaagcg cgccagaccg aggccatccg
      241 acgtcagctc cgcttctgat ctgataaagg ccgagctgtc ctttcttcgg gacaaggggc
      301 gtcccgtcag cgacctcgcc ccgatcgcct tttccaattc cgcgcctttc gcggatcaac
      361 tttcgcagtt catcacgggt catcccccgc tcatccgcgc gcttttcgag cagtaaccgg
      421 gtcttccggt cacggaccgt gagaagctcc gcgtgatgcg cccaggtcaa aaggtgcgcc
      481 ggcgcacctt ttgggtgcaa cagataaaac tgccggaacc gcttgatact gcttggcgaa
      541 aacccgactc ctctttcccg ggtcaggtcc tcagaaagcc tcacaagcag tcccgccccg
      601 taggccgcgc gggcggcgcc gtcttgctca atctggacaa tccgctttcc gatctcccag
      661 tgcgcgtgaa ccagcgtccg ccgcgcgcca tcacagagac cgacaatctc cgcgagaagc
      721 tttcgatagc tggaaggcac gatctgaccg ttcgacataa gcggaattat accccagaac
      781 accggagaca cattacataa ttagcggaag gatagtatct tgcttgcttt tgaaatttga
      841 aaaaggagag tatcgcaaat ctttctcaag gaggtcgttg ctacaaaata gtaataatta
      901 tttggagggg attttaaaat tgaccttcat gctcacagcg tgaagcagat agcctccaaa
      961 cgcacccgcc actaccgaaa acagatatcc gaacatgaca tccctcccag ctgcgtagcc
     1021 aaaaatcatc gactaatcat gctttgctag attttctcat cactgaaacg acggacataa
     1081 tcacgatata aaaaacggcg ataccgcgcc ttccccgtaa tttcatacaa aatcccggct
     1141 ttgacaaatt tttcaatcaa ggcgcttaca ttagctttcg aagtaatccc tgtcatctta
     1201 ccaataagag cggcatccac aacgggcttc cgataaaggc cattcagaaa tttcaaagca
     1261 gtttccgcat ttctgccaaa accagaaaca atcctgacgt tctcctgatg gagttttgta
     1321 atatttttcg cgactgtcac ggcttcttcc gaaacaaaac gaactccttc aagaaaaaac
     1381 ttgagccaat cccgggttcc cttctcggaa aatcggtaat cattgagctt gtcatagtaa
     1441 tcgcgacggt tttttttgaa aaactctgaa agatagagaa gcggtctcat taaaatcttc
     1501 tcttggcaga gatagaacgt gatgagaagt cttcccgttc ggccattacc atccaagaat
     1561 ggatggatgg tctcaaattg cgcatgaatc aaccctgcct ttatgagagg cggtagcttc
     1621 gtcgcgtcat gaaaaaactt ttcgagatcc gaagccgcct cttgcatctc atgcggcgga
     1681 ggaggaacaa agacagccgt ttcaattgtt ggtccaccaa tccagctttg gctacggcgg
     1741 aactctcccg gcgttttatg ctcgccacgc actcctttaa gtaatttcgc atggatctct
     1801 tttacaagac gccaagacag cggcaatgtt ccgagcctgt tcaaaccgta attcatggcc
     1861 tcaatataat tcttgatctc atccacatcg gaaggcgtcc ggtcatccgc aagttttacc
     1921 tccgccttaa catagtccag tagcgtcgct tgcgtaccct caatttgact cgaaagagca
     1981 gcttctttgc ggacatacat aaaaataaaa aaatccacat ccggaacaag ctgatcaatc
     2041 gcgtttaatt ttccgatagc cctatctgcg agagaaagca aaaaaatcag gtcgttatcc
     2101 cattcaaccg ggacggatga cggaaaaggc tctggtacga atgccttgta tcccgtgggc
     2161 tgttgccgat aaatcccaat ttttgccata tgcgcctcca gaacttacat ttcccttagt
     2221 ttcgtatgag cattatatcc agaacttata tttccgcaag ttctgcttca aaaaaaccaa
     2281 aaaaaaacat ccccgccgtt agaacatcgg cggggatttg tagaggagag tcaggacatc
     2341 gagaatcggt cgggtataca agtcccaaag ttcagccaaa aagtcttcga taatactttc
     2401 attcatttac cggacttttg aggtcctgat ctcccttaag ataaacagga atcagggcat
     2461 ccaaaagtct ttaacagtac tttcatctta cctcttcatc cggacggcgt gtcgcgtgtc
     2521 cagcaaaaga ccgttcagga aaacttccag atccttcatt taattggcgg gaatagtatc
     2581 atgcttttga aaggattagc cttaatgcaa cgtaagtcac tcgggaatca tttttttgag
     2641 aatttttccc gaccagacga ccccgattcc ggccgcaagg agatcggtca acaaccgccc
     2701 ccagaaaact ccggagagcc caaaccattt cgcccccaaa aacgccagcg gaatcatcag
     2761 gaccaaaacg cgaatggcat tcaggatgat ggccatcatc ggccggtgaa tgccggtcat
     2821 gcaaaacgtc gcgtaccggt gtacttccag aaagccgtat ccgaagcagg tgatgtaaat
     2881 ataacgcgcc aggacatcca ccacggcggg atccgaagaa aacagccgtc cgaggggctt
     2941 cgcgacaaac agaagaaccc cggcgctcac gagcccgaac gcaagcgcga aaacaaaggc
     3001 tcccctccgc accgtctgga tccggtcaaa acgtttcgcc ccgtaatttt gcgcgacgaa
     3061 cggaaccaag gacatcccga ccgtcatcgg gatcatgaac gcgaacattt caatccgggc
     3121 cgcgaccccg accgcggcca cggccgaccg tccgaaaccg gcgacgatcc tgatgactat
     3181 cgcggccgat accggggtca ggacggagct taccatcgcc gacgacccga tctgtaaaat
     3241 cctgtaccag gacgccatca tccgccgccg ggagtaagtc aggaaagaga ttagatgata
     3301 tttccggtga acaaaataat acgccgccgc catcacaaac aattcggcga tcaccgtcgc
     3361 cagcgccgcc ccccggatcc ccatcctcgg gaaaaacccc cagccaaaaa tcatcaccgg
     3421 gtcgagcacg caattcagga gcgtccctcc gaccatcaaa aaactcaccg tcttgctatc
     3481 ccctgtcccg ataataatgt ccgccaacat gatgtgtaga acccggaaaa tgagtcccca
     3541 gtaccaaatc gtcatgtatt gccgggccag ttcaagcacc tccccggcgg cgcccagccg
     3601 gagaaacagc ggctcaaagg tccacaagcc ggcccccgtc aggatgagcg aaatccccga
     3661 cgccaaaaaa atggcgtgcg tcgtaatcct cgccgcggct tgatgtttcc gccttcccag
     3721 cgcgtgggcg accaccgtca tcgcgccagt ccccactccc cgcatcacaa agccaacgaa
     3781 catcaccacc ggaaaagtaa acgacatcgc cgccagcgcc tgagtcccca accgggaaac
     3841 aaaccacgtg tcgaccatgt tatacatgtt catggcgaag gtcccggcaa gcatcggaac
     3901 cgccatacga aaaaccgtca aaaacacttt atccttaacc aaacgagaca tccaaccccc
     3961 aacttgttaa aacgttcttt ctcatatttc aaaaaatgga cgaaataccc ggaacttcac
     4021 tccttaagat ccgcgcgagg atcgcgggcg tgggcgagtg gcaagcaaaa accatcacga
     4081 cgggaggaaa acttcttcaa aacttcgcgc gtcaggccaa ggctatccgc gcacctgcaa
     4141 tttcgggaaa atggctttaa gccggtcttg caaatccgct gaaatgacgg ctccgggctg
     4201 actgcttgcg cgaagctctg gatcggggca tccctcttgt cgaaatctct ggacgtgaaa
     4261 ctctctgacc ccaagatcat gaagctcagt ggcaagctga agcaggcttt catttttcaa
     4321 caaatccgga tgcacggtcg tgcggcattc ataaggaaca tggcttgaaa gaatccgccg
     4381 cagactggcc ttcgctaccg ccccgctccc ctcacgcgtc gtcacattgg catattgatc
     4441 aaacggggcc ttgatatcga ttccaaccca gtcgcagagg ggcaaaacct ctcccagacg
     4501 atcggggtac atgccattcg tatgaagcgc gacctgatag cccatggact taaccgcctt
     4561 gacccacctg gtgaggccgg cgtgagcggt cggttctccc ccggaaaaca caatcccgtc
     4621 aagtcgtcct tgccgggatt tcaaatgaga aaggatttta tccggtgaga tccggttgcc
     4681 tcccggagga aacggccaaa gctccgaatt gtagcagtac cggcaacgcc atgagcaacc
     4741 ctgaagatag aaaaccccgg ccagacggcc ggggaagtct atcgtcgtaa acggcgttat
     4801 tccaccgaca ggtatttcag aaaggatggc acgaagttcc ggatcaccta gctccgcagt
     4861 cgcacgcgca cgcggattct ttgaagtatt ttcgctcata aaactcgcct tttttccccg
     4921 tgttgaaccc cgcgaccgga cggtgataac ccatcacacg ggtccacact tcgcagcgag
     4981 tccgttcttc atcttttagc acaattcgtt ctttcgcttc cgctaatgtc atgccagttc
     5041 ctccttggat tctgttggct caccactccc ctgcaaggcg agcatctttt catgaataag
     5101 ttcctcatcg catttggggc aaaattcgtg ttcgcccgag agatacccgt gccgcgggca
     5161 gattgaaaac gtcggcgtaa tcgtaatgta agggatccgg taattcgtca gcgcgcgctt
     5221 gatgatctcc ttgcaagcct catgcgacga aactcgttct cccatgtaaa ggtgcaaaac
     5281 cgttccgcca gtgtagcgac gctgaagttg ctcctgacgc tcaagggcct cgaacgggtc
     5341 atcggtaaaa gccaccggca actgggacga attcgtgtag tacggacgtt cgctggttcc
     5401 ggcgtgcaaa atatcgggaa accgtttgat gtcctccttg gcaaaccggt aagtcgcgcc
     5461 ttcggcggga gttgcttcca ggttgtaaag atgccccgtc tccgtttgaa actcggtcat
     5521 cctcatccgg atatgatcca ggagcttgat cgcgaattta aacccttcct cggtcgtgat
     5581 gttctcttga tcgttcgtaa aattccggat acattcattc acgccgttta acccgatcgt
     5641 ggagaaatgg ttccggagcg tcccgaggta acgtttcgtg taggggaaaa gcccctgctc
     5701 gatgtgacgc tgaatgatct ttcgcttcag ttccagactg gttttcgcga tttgtagcaa
     5761 ataatccacc cggttaaaaa gggcgctctc gtcccccttc gccagatatc cgatccgggc
     5821 gcaattgaga gtcacaacac caagtgagcc ggtttgctcc gcggaaccaa agagaccgtt
     5881 cccccgcttg agcagttcac gcaaatcaag ttgaagccgg cagcacatcg agcgaatctg
     5941 gtttggctta agatcggaat tgagaaaatt ctgaaaatac ggaagaccgt acttggccgt
     6001 catttcgaac agaagttcac agttcggatg atcccagtca aaatccggag tgatattgta
     6061 ggtcgggatc gggaacgtga atacccgtcc cttggcgtcc cctttcatca tgacctcaat
     6121 gtacgccttg ttgatcatgg ccatttcctc acccagttcg ccgtaagtaa aatccatttc
     6181 cttcccaccg acgattggat gctccgcgcg caaatcctcc ggacagaccc agtcaaacgt
     6241 cacgttcgta aaaggcgtct gggtccccca ccgcgacggc acgttcaggt tgtagataaa
     6301 ttcctgcatg ctctgcttca ccttctcgta agggagcttg tcgatccgga tataaggcgc
     6361 gagatacgtg tcaaacgacg aaaacgcctg cgcgcccgcc cattcattct gcagtgtccc
     6421 gagaaaattg accatctgcc cgaccgccga actcaggtgt ttgggcggcc cggattccac
     6481 cttgtccggc acgccgttaa atccctcacg caaaagagtt ttcagcgacc atcccgcgca
     6541 atagccggac agcatgtcaa gatcgtggat atggacgtcg ccgtggcggt gagcgttaga
     6601 aacttcttcg ggatagacgt gggagagcca gtaattggcg atcactttgc cggagatatt
     6661 gagaatcatt cctccaagcg aatagccctg gttggcattc gcgttcacgc gccaatcctg
     6721 tttgtagagg tattcattga ccgaggaaat gacatcgacg acggtttttt tatcgccccg
     6781 cagttttttg tgctgttcgc gataaataat gtaggcgcgc gcggtcttga ggtaattcga
     6841 cgtgatgagg acctgctcga caatatcctg gatggcttcg atatcgagaa aaccgcggtc
     6901 atatctgtta tcgatcacct tgatcacggc aagcgtcaga aactgggctt cggtcccgtc
     6961 ataatccccc gtcgccgcgc cggctttcag aatggcgtca taaatcttga ccccgttaaa
     7021 tgggacaatc gagccatcac gtttacggat attggtgatc tttcgagact ggaactgctc
     7081 cgccatattc acttccccct ttaaagcatt tatcgacaaa tacgccagat attgtagtta
     7141 aaatttgcca caccccaaca ggtgggggat actatcagat tttgcgttcg tgtcaatccg
     7201 ttatttggga tttttgagag gattttttcc cgcgagagcg ttaagcgcgc ggaaataacg
     7261 aaaacaaaat tttattcgcc aagttccgct ttttgtttga tcctttgaac gatcaaccac
     7321 gcgatggttt caatcgtcac gcggtccgta ttccagatcg cgtcataaag aagcgggtct
     7381 tgaacatcct gatgaaaaaa agtcttcacg agtagcgcct tggccttgtc ctgctccgcg
     7441 attttctcgc gggcccactt ctcatctttg ccgtgggcgc tcatcatcag cgcaattctg
     7501 gaggataacg gagcgacaag acgaatgtga attccatgcg gcagatcgcg agtcaggcag
     7561 gtcgatccgc ggccaatcag aatggttttt ccgaaaatcg cgagatcacg catcaatgaa
     7621 aacatcttcc gcatgacctt ttcctgcgga ctttctcccg taatgaattg cgtgagcata
     7681 tcctcgaccg cggaacgata ctccgaaaca atcagcgcct ggggagaaat cttcaactgc
     7741 ggatcctggg caaccctctc gcaaagatca tggtcgcaaa tctcccagcc ctggaaaaga
     7801 gggttttctt tttctttgag aaggaattcg acaatcttgg cggcaagatt atgccccccc
     7861 gccccggtct cgcgggagat ggtgacaaaa ggaaactgcc ctgatcgggg atcaatggat
     7921 ggtaactgct tctcgcggta ctggatcgtg tcaataaaag accggaattt cttaaactcg
     7981 ctcatatcag actcctcgcg cgaggcccac agcactcgca tccgaagttc ccttccccgg
     8041 gcggcttatt tatttcagaa tgatgcgcgc gtcaatcacg ccagcgccct tgccttcagc
     8101 gtaaaccata agcggattga tgtcaatttc ctcaatttcg ggatggtcgc aagaaagctg
     8161 ggaaagccgt ttgaggcaat cctccacggc cgcaagatca gccggcggtt ccccgcgaat
     8221 cccctgaaga agtttcgctg cccggatatt acaaaccatg tcgcgcgcgc ttaattcacg
     8281 a
//
LOCUS       DAOSIO010000059         8262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_8083_length_8262_cov_4.212989, whole genome shotgun sequence.
ACCESSION   DAOSIO010000059 DAOSIO010000000
VERSION     DAOSIO010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 8262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8262
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8083_length_8262_cov_4.212989"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PK590_03390"
     CDS             complement(<1..763)
                     /locus_tag="PK590_03390"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOG23673.1"
                     /translation="MSSLFDELIRERLISSEQLADAKMKQIGAKKPVHELLVDMGFLK
                     EEDLLKTASRIYKMPLSDLGQETIDPEALRLLTHEIAKRYGVFPVRREEDALVLAMSN
                     PQDLMAMEQVRMRTKLKVKPTLCTKSDINKAINKYYQTDDSIYDILKNVPDVAPFEII
                     REEENGEVVIDQSSMKDEASPIVRLYNLIMKDALKARASDIHIEPEENQVKVRYRVDG
                     DLRNIMEIPAQLQVELAARIKILAGMDVVEKRKPQD"
     gene            complement(992..1065)
                     /locus_tag="PK590_03395"
     tRNA            complement(992..1065)
                     /locus_tag="PK590_03395"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1029..1031),aa:Ile,seq:cat)
     gene            complement(1168..1572)
                     /locus_tag="PK590_03400"
     CDS             complement(1168..1572)
                     /locus_tag="PK590_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG23674.1"
                     /translation="MTENSKSILVIDDEDDVCQIVKARFESLGYKVLTANEGVEGFRK
                     IETSKPDVVLVDVRIPQGEDGLTFLRRLRSYRHDDIALQTRIRKTPVIILTGVGATMR
                     SLFELEGISDYIEKPFDLVNLQTKVEQVLQSS"
     gene            complement(1635..3374)
                     /gene="rpoD"
                     /locus_tag="PK590_03405"
     CDS             complement(1635..3374)
                     /gene="rpoD"
                     /locus_tag="PK590_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOG23675.1"
                     /translation="MKAKKIVKKPVSKVKPIAKVSRAVSSKISRKTSKNSGKKKKSGS
                     GKLSKAVPAATLSPKEMETVLERLLVIGKKQGFVTIEQMKKNLPTEMQGAEKVEEIIA
                     KLNEKKIQVTSESVGEQGKSTIDSLHDVDTKKTKSSELEEDGIEEPEKVRIDDPVRTY
                     LRQMGQISLLTREQEIAIAKKIEDCEEDLKKAVYQTHAARYELLELARKILNGEIQLE
                     EIVDEDQEVKIQTIKKKMKQLIRKFRASRKQDNVSGILLQFNLSIAIVEQIIATLRAK
                     LRELRKNRDEIKKVRKRRIRGKIGLLEERNQQIIKELGGSEAELLEHFKEIIKREHEL
                     SKAKKALVAANLRLVVSIAKKYTNRGLSFLDLIQEGNIGLMKAVDKFEYKRGYKFSTY
                     ATWWIRQAITRSIADQARTIRIPVHMIETINKFFRASRHLVQETGREPTPEEVAHVMK
                     MPLDKVKGILKIAQEPISLQTPIGDEGDTTFGDFIEDKSAVSPANATAYSMLKEQMDD
                     VLLTLTEREEKVLRLRFGIGDGYPRTLEEVGHIFNVTRERVRQIEAKALRKLRHPTRS
                     RKLKNFLDIKLSE"
     gene            complement(3765..5381)
                     /locus_tag="PK590_03410"
     CDS             complement(3765..5381)
                     /locus_tag="PK590_03410"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG23676.1"
                     /translation="MKVLLINPKFAPTIWSFEGLRPFTGTSFSTTPLGLATVAALTPS
                     PWEVEIIDENVEAINFDKPADLVAISAFNVQYYRALEIAREFQSRGRKVAFGGPYCSL
                     FPEAFEGKADHRIVGEAEEIWPLFLRDFEEGKARELYVAPKASVDIRKTPVPRYDLLK
                     GNRYNMFALQTTRGCPYSCEFCDIIITDGRKPRTKTVSQVIAEVDHCVQQGAHYIVFG
                     DANFIGNINFARELLRALADYSRENDYPIEFSCELTINVAHHPDLLELLQEANFYSVF
                     VGIESPRKQSLTEAGKLQNTREDILEDIKRIQSYHISVVAGMIVGFDSDDRLIFKETY
                     DFLQTLGIPFTTCGTLVALPNTPLLKRLEKEGRLLDLEWTDMNGHGASDCNFIPKQMT
                     SQELRQGYNWLSRCLYRYDSYADRLVTALSRFRNRNKEHKRARLNGNFLWLLLKVFAY
                     YTLTWEKGRIRFFYRTLWRVATGGPFSVGKWLEFFRWIATHRAFRKYILETQGDPETM
                     DPNTPPFSSSQSEIPEKLDILEEVSVPVGK"
     gene            5509..6282
                     /locus_tag="PK590_03415"
     CDS             5509..6282
                     /locus_tag="PK590_03415"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOG23677.1"
                     /translation="MSLKFFRLNMKVIVWITIVALVLLFGAGSIVDIVRSDKSGRYAG
                     EAFGKPVTFQEFNRFFRATELFMPISDRSAINDPDLLRSFTWQNIVYAREARNVGITV
                     SDGEVREQVTKILDQHGLSNPTPEQYRIWLTRNLQINPREFEESLREFMKIQKLLGRQ
                     LSNPPTNPPVEAETEKGAETPKQTEEEALQKRQIAFMTWTHELNQKAAVKDYLALPEE
                     MPKSEVPVTSPDETHPVSEKVSPSPESRKEEIENETDRR"
     gene            6263..7309
                     /gene="lpxD"
                     /locus_tag="PK590_03420"
     CDS             6263..7309
                     /gene="lpxD"
                     /locus_tag="PK590_03420"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:TIGR01853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOG23678.1"
                     /translation="MKLTVDEIARIVNGSVTGDSKILIEGITNIENPLINHITFVQDE
                     KNFKSLESSEITCLIVPKQITQSSKVLIRVDHPKRAWAKLLREIFPARKFIPGISPQA
                     VVAKSATIGSNVTIDAFVSIGENTVIGDGAVLRSGTFLDDNVKIGKNCVIHPNVTVYR
                     DSDIGANVIIHAGTVIGADGFGYVATETEQEKLPQVGNVVIEEDVEIGACCTIDRAAL
                     GSTRIGKGCKIDNLVQIAHNVVIGPHTVISAQTGISGSCKIGAHVTMGGSVGLGDHVE
                     IGDWAIIGAGAGFPSNKKVPGKQVYFGQPARPYQEMRKQFGAQLRAAETLDDVRALKK
                     KVAELEKKLDQTIS"
     gene            complement(7345..7968)
                     /locus_tag="PK590_03425"
     CDS             complement(7345..7968)
                     /locus_tag="PK590_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23679.1"
                     /translation="MKNKKQIIFYGMIALLFMQIPMLKADSETKIKREFQLTTYYPAP
                     HGDYENINTTEDTRLATSSGNVGIGTTSPHKDAKLEVNNTLRLTPVTGNAAAQKGGAE
                     GAIRYSKDAGGANVGGLLYKNASGWMPLAGTSQGQGTVVTRWNHAGTQLALNGGYTKG
                     VSIGEHLFCAIANVQNAEDAHYCRVYKSGKNWYIENRKTACQAVCLD"
     gene            complement(8145..>8262)
                     /locus_tag="PK590_03430"
     CDS             complement(8145..>8262)
                     /locus_tag="PK590_03430"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HOG23680.1"
                     /translation="GVKKGDAYTVTSSMLGAFRRDARSRSEFLSLVRLKREE"
BASE COUNT         2070 a         2223 c         1858 g         2111 t
ORIGIN      
        1 catcctgagg cttccgtttt tccacgacat ccataccggc gagaatttta atccgcgcgg
       61 caagctccac ctgtaattgg gcgggtattt ccatgatgtt ccgaagatcc ccatcaaccc
      121 gatatcggac ctttacctga ttttcttccg gttcgatgtg aatgtcgctg gcccgggcct
      181 tgagcgcatc cttcatgatc aggttataca gacgaacaat tggagacgcc tcgtccttca
      241 tggagctttg atcgataaca acttctccat tttcttcttc ccggataatt tcgaacggag
      301 ccacatcggg aacatttttc agaatgtcat agattgaatc atccgtctgg tagtatttgt
      361 tgatagcctt gttaatatcg ctcttcgtgc aaagggtggg tttcactttg agtttcgtcc
      421 gcatcctcac ctgctccatc gccatgagat cctgggggtt actcatggca agaacaaggg
      481 catcctcttc gcgcctgacc ggaaataccc cgtatcgttt ggcaatctcg tgcgtcagca
      541 agcgaagggc ttcaggatca atggtttctt ggcccagatc agatagcggc atcttgtaaa
      601 tcctggaggc cgttttaagg agatcttctt ctttcagaaa ccccatatcc acaaggagtt
      661 catggacagg tttcttggct ccaatctgct tcattttcgc gtcagcaagc tgctccgaac
      721 tgatcaaccg ctctcttatc agctcgtcaa acagagaaga catgatccac ttcctttttt
      781 tgagaatctc tcagcaaaat aatgggtatt gtgttttaaa aagactatat cgtccagtaa
      841 aatttaaaac gtagatgggg tttccccctc gaggcactga actgtcatca ttcttggcac
      901 gacaaccacg ccaatcctgg agaattttcc gacacaagag gaaaagaaaa acggggaatt
      961 atgataaaaa aacataaacc taaaatgaaa ctgggcccgc ctggacttga accagggacc
     1021 aagcgattat gagtcgcctg ctctaaccaa ctgagctacg ggcccccaaa aaacacagtg
     1081 gcactccttt atatttcgat ttagggttag gatatcggag aaaaaagagc ttggcaataa
     1141 aacaattgct tcggagaaag aaatttttca ggaggattga agtacttgct caactttggt
     1201 ctgcagattg acgagatcaa aaggtttttc aatatagtcg ctgattcctt caagctcgaa
     1261 cagggatcgc atggtcgccc cgacccctgt aagaataatc acgggcgtct tccggatccg
     1321 ggtctggagg gcaatatcat catgacggta ggaacgcaac ctgcgaagga atgtcaggcc
     1381 gtcttctccc tgggggatac gaacgtccac aagcacaaca tcgggtttgg acgtttcaat
     1441 tttcctaaat ccttcaacgc cttcattcgc ggtcaaaacc ttgtatccaa gcgattcaaa
     1501 tctggcctta acaatctggc aaacgtcatc ttcatcgtct atcacaagaa tactttttga
     1561 attctcggtc atatctgttc ctcacttgag agacttgcca acgcgcccga caaaacccgc
     1621 gcggagacat tgtattattc acttagtttg atatccagaa aattcttaag ttttctggag
     1681 cgtgtcggat ggcgaagctt tcgaagagct ttcgcttcaa tctgccgcac acgttcacga
     1741 gtgacgttaa agatgtgccc gacctcttcc agagtccgcg ggtacccgtc cccaatccca
     1801 aatcgcagac gaaggacctt ctcctcccgc tcggtgagcg tcaaaagaac atcgtccatc
     1861 tgctccttga gcatgctgta ggccgtcgcg ttcgccggcg agacagccga cttgtcttcg
     1921 atgaagtcac caaaagtggt atccccctca tccccgatcg gggtctgaag gctgatgggc
     1981 tcctgcgcaa ttttgaggat ccccttcacc ttgtcgagcg gcatcttcat gacatgcgct
     2041 acttcttcgg gtgtcggttc gcggcccgtc tcctgcacca gatgccgcga cgcacgaaag
     2101 aatttattga tggtctcgat catgtgaacc ggaatacgaa tggtccgcgc ctgatccgcg
     2161 atcgagcggg taatggcctg gcggatccac caggtggcgt aggtcgaaaa cttgtagccg
     2221 cgcttgtact cgaatttgtc gaccgccttc attagcccga tattgccctc ctggatcagg
     2281 tccaggaacg acaagccgcg gttcgtgtat tttttagcga tactcaccac taaccggagg
     2341 ttcgcggcaa ccaaggcttt tttcgccttg gacaactcat gttcgcgctt gatgatctct
     2401 ttaaaatgct cgagaagctc ggcctccgac ccaccaagtt ccttaataat ctgctgattc
     2461 cgttcctcca acaaaccaat cttcccgcgg atacgccgtt tgcgaacttt tttaatctca
     2521 tcccgattct ttctgagttc acgaagcttg gcccgaagag tcgcgataat ctgctccacg
     2581 atcgcgatgc tcaaattaaa ctgcaacaaa ataccggaaa cattgtcctg cttgcgggaa
     2641 gccctgaatt tccggatcag ttgtttcatc tttttcttga tcgtctggat cttgacttcc
     2701 tggtcctcat cgacaatttc ttccagctgg atttccccat tcaggatttt tcgggccagc
     2761 tcaagcaatt cgtaccgggc cgcgtgagtc tggtaaacgg cctttttaag atcttcctcg
     2821 caatcttcga tcttcttggc aatggcaatt tcctgctcgc gggtcaaaag ggaaatttgc
     2881 cccatctgtc tgagatatgt tctgaccgga tcatcaatcc gcaccttctc cggctcttcg
     2941 atcccgtctt cttccagctc ggaacttttc gtctttttgg tatcgacatc atgcagggag
     3001 tcaatcgtgg acttcccttg ttcccccacc gattcacttg tcacctgaat tttcttttca
     3061 ttcagcttcg cgattatttc ttctactttt tcagcccctt gcatttcggt cggcagattc
     3121 tttttcatct gttcgatagt tacaaaaccc tgtttcttgc cgatcaccag aaggcgctca
     3181 agaaccgtct ccatttcttt gggggatagc gttgccgccg gtacagcctt ggacagtttc
     3241 ccggaacccg acttcttctt tttaccagaa tttttagaag tcttcctgga tatcttggaa
     3301 gaaactgcgc gggacacttt ggcaataggt ttcactttgg aaacaggttt tttaacgatc
     3361 ttcttcgctt tcactcagac agatctccga tttgattgct tttgttgagg atacacgcaa
     3421 atgaggggcc tggaaatctt tccaaaccac tcgccctgag ggacaccctg ttctgaagga
     3481 caacatcaag ggtctccttg ataggatcct tcaaataaac cgccgctaca gactttcaga
     3541 aaacatccgc acccaaggta ggctcgaggc aatttcaccc atatttccaa cctgttgttt
     3601 ccccgaagat cggaaaaaca gaaatagctt catgaaaccg cttctaaaaa ccggcggggt
     3661 ttacagagtg gtgtattata tttatgaaaa aaaagaatac aaaggttatt tgcggcagaa
     3721 atgacggtaa tgaaagtgtt tcggaaaacg tgatataccc gtctctactt gccaaccggg
     3781 accgatactt cttcgaggat gtcaagtttc tcgggaattt cactctgaga acttgaaaac
     3841 ggaggggtat tcgggtccat tgtctcagga tccccctggg tctccagaat atacttcctg
     3901 aaagcgcggt gggtggcaat ccagcggaag aattccagcc acttccccac cgaaaacggc
     3961 ccaccggtcg cgacacgcca cagggttctg taaaagaaac gtatccgtcc cttttcccat
     4021 gtcagcgtgt aataagcgaa taccttcaaa aggagccaga gaaaattccc attcagccgg
     4081 gcccgtttat gttccttgtt tcgattgcga aaccggctta gcgcggtcac caaacgatcc
     4141 gcgtagctat cataacgata aagacatcga ctcagccaat tgtatccttg ccggagctcc
     4201 tgcgaagtca tctgtttggg aataaaattg cagtcggaag cgccgtgccc gttcatgtcg
     4261 gtccattcaa gatccagaag ccggccctcc ttttcaagac gcttgagaag cggcgtgttc
     4321 ggtaatgcca ccagggttcc gcatgtcgtg aacgggattc cgagtgtttg aagaaaatca
     4381 tacgtttctt taaaaataag ccgatcgtcc gaatcgaacc cgacaatcat gccagccacc
     4441 acggaaatgt gataagactg tatccgtttg atatcctcaa ggatatcttc acgagtgttc
     4501 tgaagtttgc cggcctccgt caagctctgc ttgcggggcg actcaatccc cacaaatacg
     4561 gagtaaaaat tagcttcctg gagaagttca agcagatcag ggtggtgcgc cacattgatc
     4621 gtcagctcgc aggaaaactc gatcggataa tcattctctc tggaataatc ggccagcgcc
     4681 ctcaggagct cgcgggcaaa attgatgttc ccgataaagt tcgcgtcccc gaacacgatg
     4741 taatgcgcgc cctgttggac gcaatgatcc acttccgcga tcacctgtga cacagtcttc
     4801 gttcgcggct tccggccatc cgtaataata atgtcacaaa attcacagct atagggacaa
     4861 ccgcgcgtgg tctgcagggc aaacatgttg taacggttac ccttcagaag gtcatagcgc
     4921 ggcacgggag ttttccggat atcaacactg gccttgggcg cgacgtaaag ttcccgagcc
     4981 ttcccctctt caaaatctct caggaaaaga ggccaaattt cctcagcctc accgacaatg
     5041 cggtgatccg ccttgccttc aaaagcctca ggaaaaagac tgcagtacgg gcctccaaac
     5101 gcaaccttcc ggccacgcga ctgaaattcc ctggcaattt ccagggcgcg atagtactgg
     5161 acattaaacg cgctgatggc caccagatcc gccggcttgt caaaattgat cgcttcgacg
     5221 ttctcatcaa tgatttcaac ttcccagggg gatggagtca gggccgctac cgtcgcaagc
     5281 ccaagaggcg tagtcgaaaa actcgtaccc gtaaacgggc gcaaaccttc aaacgaccag
     5341 atcgtgggag caaatttggg attgattaaa aggactttca ttggcgaagt ataccacttt
     5401 tcctgccgga atctggcaaa tatttatatt tttttcattc acggttgtac ggttatcaaa
     5461 cattttattt tcgaaaaagg aaccttttat gctacattat gcgccattat gtcgctcaaa
     5521 ttctttcggc taaacatgaa ggttattgtc tggattacca tcgtcgctct cgtactgctt
     5581 tttggagcag gatccattgt cgacatcgtc cgctctgaca aaagcggccg atacgccggt
     5641 gaagcattcg gtaagccggt taccttccag gaatttaaca ggttttttcg tgccacagag
     5701 ctcttcatgc cgattagcga ccgctccgct atcaacgacc cggatcttct gcgctctttc
     5761 acgtggcaaa acatcgttta tgcccgggaa gcgcgaaatg ttggaatcac agtctcggac
     5821 ggcgaagttc gtgaacaagt cacgaagatt ctggaccaac acggactttc caatcccacg
     5881 cctgaacaat accgtatctg gctcacccga aaccttcaga taaatccccg ggaattcgag
     5941 gaaagcctcc gggaatttat gaaaattcaa aagctccttg ggcgacagct ttccaacccg
     6001 cccaccaatc cacccgtgga ggcagaaacg gaaaagggcg cggaaactcc gaagcaaacc
     6061 gaagaagagg ccctgcaaaa acgtcagata gccttcatga cttggactca cgagctcaac
     6121 cagaaagcgg ccgttaaaga ctatctcgct ttgcccgaag aaatgccaaa atctgaagtt
     6181 cccgtaacca gcccggatga gacccatccg gtttcagaaa aggtttcacc ctcacccgaa
     6241 tctcgaaagg aagaaatcga aaatgaaact gaccgtcgat gaaatcgccc ggatcgtcaa
     6301 cggctctgtc acaggggatt caaaaatcct gattgagggc attaccaata tcgaaaatcc
     6361 cctcattaac cacatcacat tcgtccagga tgaaaaaaac ttcaaaagcc ttgaatcgtc
     6421 cgaaattacc tgtctgatcg ttccgaaaca aatcacccag tcttcgaaag ttctaatccg
     6481 ggttgatcat ccgaaacgcg cgtgggccaa actcctccgt gagatattcc ccgcccgcaa
     6541 attcattccc ggcatttctc cgcaagcggt cgtcgccaaa tccgccacga tcggtagcaa
     6601 tgttaccatc gacgccttcg tctcaatcgg tgaaaatacg gtgatcgggg acggtgcagt
     6661 tctacgctcc ggtacattcc ttgatgataa cgtaaaaatc ggcaaaaact gcgtgattca
     6721 cccgaatgtc actgtctacc gggattctga catcggcgcg aatgtcatta ttcacgccgg
     6781 caccgtcatt ggcgcggacg gcttcggata tgtcgcgacc gaaacggaac aggaaaaact
     6841 tccccaggtc gggaatgtcg tgatcgaaga ggatgttgag attggggcct gttgcaccat
     6901 cgaccgggcg gctctcggat caacccggat cggcaagggc tgcaaaatcg ataatcttgt
     6961 gcaaatcgcc cataacgtcg tcattggacc ccacacggtc atttcggccc aaacgggcat
     7021 ttcaggcagt tgcaagatcg gcgcccacgt caccatgggc ggaagcgtcg gcctcggcga
     7081 ccatgtggaa atcggggatt gggccataat cggcgccgga gccggatttc cttccaataa
     7141 aaaagttccc ggaaagcagg tctatttcgg acagccggca cgtccctacc aggaaatgcg
     7201 caaacagttt ggcgcgcaac tccgcgccgc ggaaaccctt gatgacgtcc gcgcactcaa
     7261 gaaaaaagtc gcggaactgg aaaagaaact agaccaaacg atttcttaaa caaaatctcc
     7321 tggctacact cacttaaaac acctctaatc cagacatacc gcctggcaag ctgttttacg
     7381 attttcaatg taccagtttt tcccactttt ataaactctg caataatggg catcttctgc
     7441 attttgaaca ttggcaatag cacaaaacaa atgttctcct atagaaacac cttttgtgta
     7501 gccaccattc aaagccaact gtgtgccagc atgattccag cgggtaacca ccgttccttg
     7561 tccttgagaa gtacccgcca aaggcatcca gccgctggca tttttataaa gcaatccgcc
     7621 aacatttgca ccgcctgcgt ccttactata ccgaatggca ccttccgctc ctcccttttg
     7681 tgccgccgca ttgcccgtga caggtgtcaa acgcagtgtg ttgttgactt caagcttggc
     7741 atctttgtga ggagacgtgg tcccgatccc gacatttcct gacgaggtcg ccagtcgggt
     7801 atcttcggtc gtattgatat tttcataatc accatgcggc gcggggtaat aagttgtcag
     7861 ttgaaattct ctcttgatct tggtctctga gtctgccttt agcatcggta tttgcataaa
     7921 cagtagcgca atcattccgt aaaatattat ttgtttttta ttcttcatgt tcttcctccc
     7981 gttaaatttc aatcattgca cgccaacgca tcattatacc gttctctccc ttcagctctc
     8041 cataaaaaaa tccaggctct ggggaaaaag ctcatttctt agccgtgcgc cgcacgctcc
     8101 ctgccacaag aaaattcccg tcgccttccg gcatgtagca gtttttattc ttccctcttg
     8161 agtcggacga gactcagaaa ctcactgcga gagcgggcgt ccctgcgaaa ggcgccgagc
     8221 atggaacttg tcacggtata ggcgtcacct tttttgacgc ct
//
LOCUS       DAOSIO010000060         8146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_8213_length_8146_cov_4.736868, whole genome shotgun sequence.
ACCESSION   DAOSIO010000060 DAOSIO010000000
VERSION     DAOSIO010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 8146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8146
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8213_length_8146_cov_4.736868"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PK590_03435"
     CDS             <1..365
                     /locus_tag="PK590_03435"
                     /inference="COORDINATES: protein motif:HMM:NF016439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HOG23681.1"
                     /translation="RGFSYLRPLRLKDIAKRLELHESTISRAIHGKYMSTPQGTIPFK
                     SFFSQKIETRDGVGESQTSIVEKVKALIEREDPKKPLSDQAIAKILTQDGIQIARRTV
                     AKYREMLKILPSHLRRTP"
     gene            complement(436..511)
                     /locus_tag="PK590_03440"
     tRNA            complement(436..511)
                     /locus_tag="PK590_03440"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(476..478),aa:Thr,seq:cgt)
     gene            complement(610..1161)
                     /locus_tag="PK590_03445"
     CDS             complement(610..1161)
                     /locus_tag="PK590_03445"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOG23682.1"
                     /translation="MKPQKKYQVRNKKIIFSKGPIRLIDCDVRRPDGKVLNRQILEHP
                     GCVVIVPQTKQGRYLLVSQYRYPVKQNLWEFPAGGREPGESFENAARRELMEETGMKT
                     GRISRLLEFYPTPGVSAERMVLFLARDLTPGTANKDEDEEIRVKDFSLPDIGRMISSG
                     RICDGKTIIGYHTVKTQERSQEV"
     gene            complement(1302..1829)
                     /locus_tag="PK590_03450"
     CDS             complement(1302..1829)
                     /locus_tag="PK590_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23683.1"
                     /translation="MKKSRVKKIKPIARKDLLFHAVHGLCLVDKVTRETRGGKKVACY
                     SLVPTVMGKMKVRFLVAEPDLETSGFHKIISAKEAAKILEYLKAGDDKSEQTEQPWIL
                     ARNILCFSKDKLKARDQRKRQMLEYSVRGLTGELASAFGVSLKESASRIEKSLSKILK
                     NDSLVFTTLKRVAEN"
     gene            complement(2123..2196)
                     /locus_tag="PK590_03455"
     tRNA            complement(2123..2196)
                     /locus_tag="PK590_03455"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2162..2164),aa:Gly,seq:ccc)
     gene            complement(2241..3158)
                     /locus_tag="PK590_03460"
     CDS             complement(2241..3158)
                     /locus_tag="PK590_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOG23684.1"
                     /translation="MMRKNPSLIVVGTVAIDKLTTPFGAEQTVFGGSASFFSYAASFF
                     SQVGIVAVVGEDFPDEYRNILKERNIDLSHLEVKPGKTFHWEGKYETDMNSAITLRTD
                     LNVLLDFKPQIRFSKMPEFVFLANVDPDIQLSVLDQVHGSSLKFVASDTMNFWIQNKP
                     ESVKKVLGRVHAVFMNDGEARQLTGEVNLIRAAQRIHQMGPEYVIIKKGEHGALLYSH
                     EGVFLYPAYPLETVFDPTGAGDSFAGGVMGYLASARMVNLKIMKSAIAYGTIVASFTV
                     EKFGLARLREVKREHFDKRLEVLKKICKI"
     gene            complement(3159..3803)
                     /gene="thiE"
                     /locus_tag="PK590_03465"
     CDS             complement(3159..3803)
                     /gene="thiE"
                     /locus_tag="PK590_03465"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOG23685.1"
                     /translation="MKSSSRIFENFSLYAVTDLKGDSAGIIRKIESVYHGGADIVQLR
                     SHCLTDSQMLRIGEKVKKIARRYRRLFFINDRLDLAIALGADGLHLGQNDMPLPLARM
                     IAGRAGCRFKFGRSTCCLKQARTAIREGADYIGVGPVFETPTKPGRTPVGLEYVRQAA
                     RYVQIPWVAIGGIDLGNLALVMSAGAKRVAVVRAIFSARDPERATRTFKQKLGD"
     gene            complement(3809..4285)
                     /gene="rfaE2"
                     /locus_tag="PK590_03470"
     CDS             complement(3809..4285)
                     /gene="rfaE2"
                     /locus_tag="PK590_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOG23686.1"
                     /translation="MRPSKILTRATLPGVVAKIRRAKKRIAFTNGTFDILHLGHVTYL
                     QKAKATADVLIVGVNTDRSVKTYKDPTRPINPQNDRLQVLAALACIDYVILFDDPTPL
                     KLILAVRPDILVKGADWALKDIAGAKEVLSWGGKVRRIKLVAGRSSTRIIRALKLA"
     gene            complement(4290..4862)
                     /locus_tag="PK590_03475"
     CDS             complement(4290..4862)
                     /locus_tag="PK590_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HOG23687.1"
                     /translation="MKRYFEVFLKGHDEAFQKSFHDDSMEVLNRIAQSIINAFKAGRK
                     VILCGNGGSGTDALHVAAEFVGRFEMERMSLPAIALSADTASITAIGNDYGYDRVFER
                     QLESLGHKGDVLIALSTSGRSPNVLKAVEKAKQKGLLTIGFTGREGGLMKGMVDHCFC
                     VHSQRTAHIQEIHMIALHAICGVVEQVLFG"
     gene            complement(4866..5894)
                     /locus_tag="PK590_03480"
     CDS             complement(4866..5894)
                     /locus_tag="PK590_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOG23688.1"
                     /translation="MKRIGMLTGGGDCPGLNAVIRAIVWMAKREGWTCIGFKQGWKGI
                     IENIHEELTLDSISGILPRGGTILRTSRTNPYKKEGDVEKVRENIKKNQLDALIAIGG
                     EDTLGAGHKLFKDGIPVIGVPKTIDNDLSATDYTFGYDTALSIATECIDRLHTTADSH
                     NRVIVAEIMGRHAGWIALESGLAGGADVILIPEVPMSSEEICDFLQKRHQRGKNFSIV
                     AVAEGFSIKGEDLVTTKSELDAFGHVQLGGVGERLAKIIEKNTGFETRCTVLGHIQRG
                     GSPTAFDRVLGTRFGVKAFELAKLGKFGHMAALRGREIVAVPLADAVGALKTVPMELY
                     DMARQFFG"
     gene            complement(5935..6501)
                     /locus_tag="PK590_03485"
     CDS             complement(5935..6501)
                     /locus_tag="PK590_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23689.1"
                     /translation="MKVTSAFAQLFAIFSFLTLGSLMIIVSLHLLAFDDAILKLQEIY
                     QNPWRSVQVGVVGLVFIILGINFSKALVKSGRPNEAVIFHSEIGPMVVSSSAIEMAAL
                     KAIKHFSLVKKARVKINISGKDVELKLRLKLWAGGSVPSVLAELQEAVLSRVKRLLGP
                     ENQVTVICDVKGMEDSESALQKSGKRNK"
     gene            complement(6505..7887)
                     /gene="accC"
                     /locus_tag="PK590_03490"
     CDS             complement(6505..7887)
                     /gene="accC"
                     /locus_tag="PK590_03490"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOG23690.1"
                     /translation="MFSKIMIANRGEVAIRVIRACRELGIRTVAVFSKADRDSLHVKL
                     ADEAICIGESPSSESYLNIPALISAAEIADVEAIHPGYGFLSENAHFGEICESCQIKF
                     IGPRPESIRLAGDKSVARMEMKKAGVPIIPGSKGTIEDQEEALRLAKRLGYPVIIKAS
                     AGGGGKGIKIAHNDGKLISSFLTAQVEAEAAFGNRDIYIEKFIAKPRHIEVQILADSH
                     GNVVHLGERDCSVQRRHQKLIEESPAPGISAKLRRKIGAAAIRAAKAFEYENAGTIEF
                     LLDGDENFYFMEMNTRLQVEHPVTEFVTGIDLVKEQIRIAAGEKLKLTQEDIEIRGHA
                     IECRINAEDPDNNFMPSPGKIETWNMPGGPRVRVDSHGYAGYTVSPFYDSMLAKLIVH
                     GENRQDAISIMLRALREMQIGPIKNTISLHERILNNPKFRQGGVNTKFIETFFSPGTT
                     ETSENDGDRK"
     gene            complement(7928..>8146)
                     /locus_tag="PK590_03495"
     CDS             complement(7928..>8146)
                     /locus_tag="PK590_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017661609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HOG23691.1"
                     /translation="MVGTFYAAPAPDQPPYVSVGKAIKAGDVLCIIEAMKLMNEIKSE
                     TGGVITEILVQNGQTVEYDQPIMKIKKA"
BASE COUNT         1979 a         2304 c         1898 g         1965 t
ORIGIN      
        1 cgcggggctt ttcttatctt cgccccttac ggctgaaaga catcgccaag cggcttgagc
       61 ttcacgaatc caccatcagc cgcgccattc acggtaaata catgtccacc cctcaaggaa
      121 caatcccgtt caaaagtttt ttttcgcaaa aaatcgagac acgggacggg gtcggagaat
      181 cccagacgag cattgtcgag aaagtcaaag ccctgatcga gcgggaagat ccgaaaaaac
      241 ctctgagcga tcaggcgatc gcgaaaatac tgacccagga cggaattcag atcgcgcgga
      301 gaaccgtggc caaataccgg gagatgctaa aaatcctgcc atcacatcta cgccgtacgc
      361 cgtgagatct gaaccacaag cttatacagc ggaagtattg taacccacgt gtccgcggta
      421 tggacatcgg aaagatggag ctggaggtcg gatttgaacc gacgacctgc tgtttacgaa
      481 acagctgctc taccagctga gctactccag ccaggatgtt cgggaggcga gatgatataa
      541 caggaaagtc ggaaaagcaa actagtcttt cggccactta tcggccgcgc ggaaaaaatg
      601 ttggcgcgat caaacttcct gagaccgctc ttgtgttttg acggtatgat agccaatgat
      661 ggttttccca tcgcaaattc gacctgacga gatcatcctg ccgatatccg gcaaactaaa
      721 atctttgaca cgaatctcct catcctcatc cttattcgcg gttcccggag ttaaatcccg
      781 cgccaaaaaa aggaccattc tttccgcgga aactccggga gtcggataaa actcaaggag
      841 cctggaaatt cgtcccgtct tcatcccggt ctcctccatc aactctcgac gcgcggcatt
      901 ctcaaaggat tccccgggct cccggccacc ggccggaaat tcccaaagat tttgtttgac
      961 cgggtaacga tactggctga cgagcaggta tcttccctgt ttggtttgcg ggacaatcac
     1021 gacacagccc gggtgttcaa ggatttgacg gttcaggact tttccgtcgg ggcgtcgcac
     1081 gtcgcagtcg atgagccgga ttggcccttt ggaaaaaata attttcttat tccgaacttg
     1141 atattttttt tggggcttca tgaaaaaaag gttcctataa gcattgcgta aagctggggc
     1201 cggtataaaa tccggttcgc ggatcttctt ctcgtattcc ctccgcacaa aaaaattgag
     1261 cgcgccgcga taatgaagtg agggtatttg agaatccgcg actaattttc cgcgacgcgt
     1321 ttcaatgtgg taaaaacaag cgagtcattc tttaaaatct tcgaaagact tttttcaata
     1381 cgcgaggccg actctttcag gctcacgcca aaagcgctgg caagctcccc cgtgagtcct
     1441 cgcacggaat attcaagcat ctgacgcttt cgttgatcac gagctttcaa tttatctttg
     1501 gaaaaacaga ggatattgcg ggcaaggatc cagggttgct cggtctgctc agatttatcg
     1561 tcacccgctt taagatattc caggatcttg gcggcttcct tcgccgatat gatcttgtgg
     1621 aaccccgacg tttccaaatc aggctcagca actaaaaaac gcaccttcat cttgcccatc
     1681 accgtgggga cgagcgaata gcatgccact ttttttccgc ctcgcgtttc cctggtaacc
     1741 ttgtccacta aacacaaacc atgaacggca tggaacaaaa gatcttttcg agcaatgggt
     1801 tttatttttt ttacgcgaga ttttttcacg tcctctcctc ccttataaaa gacaaaactg
     1861 catttcccga tacttctgag ggcccagaat cacgcccagt aaaaggggcc caaaaccgat
     1921 cgatgcctcc cttccccctt gatatttacg cgaacatcat ctcgcgttgg agaagtggca
     1981 tgagttggtg acagttgatt aacaacgcag aacatcctgg tgagttatag acgcattgta
     2041 gcacgttatc ccccaaaatt ctaaccttaa ttgatttttt cggggtgggt tttgagaatc
     2101 catcaagatc gcaaaaaaga gatggagcgg gatacgggaa tcgaacccgt gttccaagct
     2161 tgggaagctt gtgtcttacc attagacgaa tcccgcgaaa aaagggaggc tgaatttcct
     2221 gccagtatcc gtttcggatt tcaaatctta cagatttttt tcaaaacttc cagacgtttg
     2281 tcaaaatgtt cgcgcttgac ctcacggagc cgcgcgagac caaatttttc aaccgtgaaa
     2341 gaggcgacga ttgtcccgta ggcgatggcg gacttcatga tcttgagatt caccatcctc
     2401 gccgaagcaa gatagcccat cacgccgccg gcaaaactgt cccccgctcc cgtggggtca
     2461 aaaacggtct caagaggata agccggataa agaaaaactc cttcgtgact gtaaagaagg
     2521 gccccgtgtt cccccttttt aataatcacg tattccggtc ccatctgatg gatgcgctgg
     2581 gccgcgcgaa tcaggttcac ctctccggtc aattgccggg cttccccatc attcataaaa
     2641 accgcgtgaa cacgtcccag aaccttttta actgactcgg gtttattctg aatccagaag
     2701 ttcatggtat cggaagcgac aaatttgagc gacgagccat ggacctgatc aaggaccgac
     2761 agttgaatat ccgggtccac gttcgccaga aaaacaaatt ccggcatttt tgaaaaacgg
     2821 atttgaggct taaaatcaag aagcacgttt aagtccgttc ggagcgtgat ggccgaattc
     2881 atgtcggtct catatttccc ttcccagtga aatgtcttgc cgggcttgac ctcaagatgg
     2941 ctgagatcaa tgttccgttc cttgaggatg ttccggtatt catccggaaa atcctctccg
     3001 acgaccgcca cgatccccac ctgactgaaa aaactggccg cgtaagagaa aaatgacgcc
     3061 gatccgccga acaccgtctg ttccgcgcca aaaggagtgg taagtttgtc aatcgcgacc
     3121 gtgccgacga caataagact tggatttttt cgcatcattc agtctcctaa tttttgttta
     3181 aacgtccgag tggcccgttc ggggtctcgg gccgaaaaaa tcgcgcgaac caccgcgacg
     3241 cgttttgctc ccgcgctcat cacaagggcg agattcccga gatcaattcc gccgatcgcc
     3301 acccagggaa tttgaacgta acgggcggct tgcctcacat actcaagtcc taccggagtc
     3361 cgtccgggct tggtcggcgt ctcgaatacc ggacccaccc cgatataatc ggccccttcc
     3421 cggatggcag ttcgcgcctg tttcaggcaa caggtcgacc gcccaaattt gaaccgacat
     3481 ccggcgcgtc ccgcgatcat tcgcgccagg ggcaagggca tgtcgttttg tcccaggtga
     3541 agcccgtccg cgccaagcgc gatcgcgaga tccagccggt cattgataaa aaaaagccgt
     3601 cggtaacgcc gcgctatttt tttgaccttc tcaccaattc gcaacatctg cgaatcggtc
     3661 aggcagtggg accgaagctg gacaatatcc gccccgccgt gataaaccga ttctatcttc
     3721 cgtatgattc ccgcggaatc gcccttgagg tccgtgaccg cgtagagcga aaaattttca
     3781 aaaatccggg acgaggattt cacgtctctc aagccagttt caaggcccgg ataattcggg
     3841 ttgatgaccg gcccgcgacc aatttaattc gcctgacttt tcctccccag gaaagaacct
     3901 ctttggcccc ggcaatgtcc ttcaacgccc aatcagcccc tttgacaaga atgtcgggcc
     3961 tgaccgcaag gatcagctta agaggagtcg ggtcgtcaaa aagtatcacg taatcaatac
     4021 acgcgagtgc cgccaaaacc tgaagccggt cattttgggg attgatcgga cgggtgggat
     4081 ctttgtaggt tttcaccgag cgatccgtat tcacacccac gatcaggacg tcagccgtcg
     4141 ctttagcctt ttgcaaatac gtgacgtgcc ccaagtgcaa aatatcaaac gtcccattcg
     4201 tgaacgcgat ccgctttttg gcgcggcgaa ttttcgccac cacgccggga agtgtcgcgc
     4261 gggtcaaaat ctttgaaggc ctcatccgct tacccgaaca ggacctgttc cacaactcca
     4321 cagatcgcgt gaagcgcgat catgtggatt tcctgaatgt gggctgttct ttgcgaatgg
     4381 acgcaaaagc aatgatccac catccccttc atcaagccac cctcacgacc cgtaaacccg
     4441 atcgtaagga ggcccttttg cttggccttt tcgaccgcct tcaaaacgtt gggagatcgt
     4501 ccgctcgtcg aaagagcgat gagcacgtcc cctttatggc ccaaactttc gagctgacgc
     4561 tcaaaaacgc gatcatagcc ataatcattt ccaatcgcgg taatcgaggc ggtatccgct
     4621 gaaagagcga tcgcgggcag gctcatccgc tccatttcaa aacgtcccac aaattcggcg
     4681 gcgacatgaa gggcatccgt cccggagcca ccgtttccgc aaaggatcac tttgcgtccg
     4741 gctttgaacg cgttgatgat cgactgggct atccggttca agacctccat ggagtcatca
     4801 tggaatgact tctgaaacgc ctcatcgtgt ccctttaaaa aaacttcgaa atatcttttc
     4861 atggcttacc cgaaaaactg ccttgccatg tcatatagtt ccatcgggac ggtcttgagc
     4921 gcgccaaccg cgtccgcgag cgggaccgcg acgatctcgc gcccgcgaag cgcggccatg
     4981 tggccaaact tcccaagttt cgcgagttca aaggccttga cgccaaaccg ggtgcccagc
     5041 acgcgatcga aggccgtggg cgacccgccc ctttgaatgt gtcccagtac cgtgcaacgg
     5101 gtttcaaatc cggtattttt ttcgataatt tttgcgagac gctctcccac cccgccaagc
     5161 tgaacatgcc caaacgcgtc cagctcactt ttagtcgtaa ccagatcttc ccctttaatc
     5221 gaaaaacctt ccgccaccgc aacgatactg aaattcttgc cgcgttgatg ccgcttttgg
     5281 agaaaatcac aaatctcttc ggaagacatc ggtacctcag gaatcaaaat gacgtccgcg
     5341 ccccccgcga gtccggattc aagcgcgatc caaccggcgt gacggcccat aatttccgcc
     5401 acgatgaccc gattatgcga atccgcggtc gtgtgcaacc ggtctatgca ttctgtcgcg
     5461 atcgaaagcg ccgtgtcgta accgaacgtg taatccgtgg cgctcagatc attgtcaatc
     5521 gttttgggaa ctccaatcac cgggattccg tccttaaaaa gcttgtgccc ggcgcccagc
     5581 gtgtcttcgc ctccaatggc aatcagggcg tccaactggt ttttcttgat gttttcccga
     5641 actttttcaa cgtccccttc tttcttgtag ggattggtcc ttgaagttct gagaatggta
     5701 cccccccgcg gcaaaatacc ggagatagag tccagggtga gctcttcgtg aatattttca
     5761 atgatgccct tccatccctg cttaaatccg atgcaggtcc acccctcccg cttggccatc
     5821 catacaatgg cccggattac cgcgttcagt cctggacaat cgccaccacc cgtcaacatc
     5881 ccgatacgtt tcatttttct caatcctcct tttaaattta aattaagtta ccttttattt
     5941 atttctcttg ccggatttct gaagcgcgga ttcggaatct tccataccct tgacgtcgca
     6001 gatcacggtc acctggtttt ccgggcccaa caaacgcttc acgcgactta aaacggcttc
     6061 ctgcaactcc gcgaggaccg acggcaccga gccccccgcc catagtttca agcgaagttt
     6121 cagctcgaca tctttaccgc tgatattgat tttgaccctg gcttttttaa ctagggaaaa
     6181 atgctttatc gctttcagcg ccgccatttc aatcgcgctt gacgaaacca ccatgggacc
     6241 aatttcacta tgaaaaatca cggcttcgtt cgggcggccg gacttgacca gagcttttga
     6301 gaagttaatg cctaaaataa taaaaaccag tccgacgacg cctacctgga ctgaacgcca
     6361 cggattctga taaatctcct gaagtttcag gatggcatca tcaaaagcaa gcagatggag
     6421 cgagacgatg atcatcagac tgccaagggt caaaaaggaa aaaatcgcga aaagctgagc
     6481 gaacgcgctg gtgaccttca tcgtctattt cctgtcgcca tcattctcgg atgtttcagt
     6541 cgtcccgggg ctaaaaaagg tttcgatgaa cttcgtgttc acgcccccct ggcggaactt
     6601 gggattattt agaattcgtt cgtgaagcga aatcgtattt ttaatcggac ctatctgcat
     6661 ttcccgcaaa gctctcagca taatactgat cgcgtcctgg cggttctcac catgcacaat
     6721 caacttggcc agcatggaat catagaacgg tgaaacggtg tagccggcat aaccatggct
     6781 gtccactcgc acgcggggcc cgccgggcat attccacgtc tcaatcttgc cgggagaagg
     6841 catgaaatta ttatcggggt cttccgcgtt gatccggcac tcaatcgcgt gtcccctgat
     6901 ctcgatatcc tcctgggtta acttaagctt ttccccggcc gcgatccgga tctgttcttt
     6961 cacaagatca atccccgtca caaattcagt caccggatgc tccacctgca accgtgtgtt
     7021 catttccata aagtaaaaat tctcgtcacc atccagcaaa aactcgatcg tcccggcatt
     7081 ttcatactcg aatgctttcg ccgcgcgaat ggccgcggcc ccgattttcc ggcgaagttt
     7141 cgcgctaatc cccggcgcgg gtgattcttc gatcagtttc tggtggcgac gctggacgga
     7201 acaatcacgc tcacccagat ggaccacatt gccgtggcta tccgccaaaa tctgaacttc
     7261 aatatgccga ggcttcgcga taaatttttc aatgtaaata tcccggttgc caaatgccgc
     7321 ttccgcttcc acctgggccg tcaaaaagga actgatcaat ttcccgtcat tatgcgcgat
     7381 tttaatgcct tttccgccgc cccccgccga ggccttgata atcaccggat aacccagtct
     7441 tttggcgagg cgaagcgcct cttcctgatc ttcgatcgtt cccttactcc cagggatgat
     7501 cggaacgccg gcttttttca tttccatgcg cgcgacggat ttatcacccg cgagccgtat
     7561 actctccggc cgcggtccaa taaacttgat ctgacaggac tcgcaaatct cgccaaaatg
     7621 cgcgttttca gataaaaatc catagcccgg gtggatggcc tcaacatcgg caatctccgc
     7681 cgccgagatt aacgcgggaa tgttcaaata actttcagat gaaggtgatt caccgatgca
     7741 aatagcttca tcagccagtt tgacgtgaag cgaatcccgg tcggcctttg aaaaaaccgc
     7801 gaccgtacga atccccagtt cacggcaagc ccgaataacg cggatcgcga cctctccgcg
     7861 atttgcgatc ataatcttgg aaaacatagc tcgtgatttc ctcttcaggt ggcaagaaag
     7921 cgccggatca ggctttcttg atcttcatga tgggctggtc gtattcgacc gtctgaccgt
     7981 tctggacaag aatttccgtg atcacaccgc ccgtttcaga cttgatctca ttcataagct
     8041 tcatggcctc aataatgcag aggacatccc ccgccttgat tgctttcccg acagagacat
     8101 acggaggctg atcaggcgca ggcgccgcgt aaaacgttcc caccat
//
LOCUS       DAOSIO010000061         7856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_8561_length_7856_cov_4.297782, whole genome shotgun sequence.
ACCESSION   DAOSIO010000061 DAOSIO010000000
VERSION     DAOSIO010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 7856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7856
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8561_length_7856_cov_4.297782"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..1324
                     /locus_tag="PK590_03500"
     CDS             89..1324
                     /locus_tag="PK590_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23692.1"
                     /translation="MIIKRIAVLSLCLIFSLPNIARAEIPWNSLNLPATQFSESSRYH
                     KVNSTHEDPHNPGFLIFSVSNDAVTAQVEGLLGLKKWIHESAVIGQLNENKNAAGGVA
                     DGAIESVKGTGRGLKNLVLHPIDSAKGLGSAAGRVGDRMSDAFRTKEEGEKGDSFLSS
                     SKRQIAQKLGVDVYSRNEQLQEKLSSLAKQGMGGRSVVMVANFFIPFGLIASTVMTVS
                     NINNAADQLVNDKDRIDLYKINKDSLMRIGIPENKAIALLNNTHYTPRQATYIRFYLE
                     RLAPLRGYRVLADRAAELTEDLPARKFLHEMQMAADGMAGMSDAVSINMVPEGLLLER
                     RDGVVLICGYDYLADSPLGKTLIEKVGSYKAKLKKNSAEIWNAGIVTPRLNGSLLAHG
                     IKVRRMCLFLNTSDGNTAT"
     gene            1411..2373
                     /locus_tag="PK590_03505"
     CDS             1411..2373
                     /locus_tag="PK590_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG23693.1"
                     /translation="MKKKRVCLVTGGAGFIGSHITDALLRKGYRVRVLDNFSRGKMEN
                     LSDSIRSIDLVRGDIRCLKTVKKMMKGTDYVFHLAAIASVPQSCEDPEETHEVNVRGT
                     LNVLLAARDAKIKRVVFTSSSAIYGDTKNFPTSEQERPMPESPYAASKMMGEYYCRNF
                     TALYGLETVALRYFNVFGPRQDPKSQYSNVIPIFIRQIKLGNPVMVHWDGRQSRDFVY
                     IENVVNANLIAMDHPLAKGESFNVGCHEEKNILGIVRELRKCMGIKSLKVKFCPKRAG
                     DVRRTLADITKAEKRMGYRPTVFFDEGLRKTVAWFLNRPEALSR"
     gene            2533..3246
                     /locus_tag="PK590_03510"
     CDS             2533..3246
                     /locus_tag="PK590_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23694.1"
                     /translation="MHIKKFLIAVLLLSGLMSPVYAESEKKFTLTTYYPAPYGDYKQL
                     KATGPDTSNATIALEAHGSSGTGLVVTNANNVGIGTSAPAAKLDIVGNGSTIIVPRKS
                     TAGDPANGVNGMIYYNSNTNKFRAYENGEWKDLIGGGGNLQCKMGSFYGNGKAVWQTI
                     NVGFRPDFVYITGYAGGGDCSRDRVKTNTMPGAYAKKIYQSSYDDDRIQLTDTGFKVY
                     YAVNCPGAPYFYVALKSNT"
     gene            3347..4828
                     /gene="gatB"
                     /locus_tag="PK590_03515"
     CDS             3347..4828
                     /gene="gatB"
                     /locus_tag="PK590_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOG23695.1"
                     /translation="MNFEPVIGLEVHVQLKTKSKLFCPCPTTFGEEGNRNTCPVCLGW
                     PGSLPVVNEEALKLAIRAGLALQCQVAERIKFDRKNYFYPDLPKAYQISQYDMPVNGR
                     GYVDVETKIDNGTDFSSGSGATTPERFVTRRIGITRAHLEEDAGKLIHEGVADGSLVD
                     YNRGGVPLLEIVSEPDIRSPREAHDYLKALKAILQYAEVSDCDMEKGSLRCDANVSVR
                     PVGQEKFGTRVEIKNLNSFKMVERAIQYEIERQTRVLEDGESIVQETRLWDDPKGMTY
                     SMRSKEDAHDYRYFPDPDLVPFTLARETVEAIRKTLPELPLDRAKRFMADYGLPEYDA
                     YTLVEDKRISEYFEDVVKAGVAAKTSSNWILTELLAVLNTKNLAIESSPVPPESFARL
                     LRLIEDGTLSGKMAKDVLGMMFDTGKDAETIVKEKGLVQVSDTSLIEKAVEKAIAANP
                     RAVEEFKAGKQKALGAIVGFVMKETQGKANPQLTNEILRRKLG"
     gene            4850..5941
                     /gene="tsaD"
                     /locus_tag="PK590_03520"
     CDS             4850..5941
                     /gene="tsaD"
                     /locus_tag="PK590_03520"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOG23696.1"
                     /translation="MKSSGKAERRKKKKKRAPLPVSRSPVYTLGVETSCDETSCAVIK
                     NGTEILSNVVSSSLFRHKKFGGVVPEIASRHSMEQIRCVFHEALEEAKIKASDLDLIA
                     VTQGPGLIGSLFVGVSFAKALAYELEIPLVGVNHMEAHLVSNFFEKPAPKRYVGLLVS
                     GGHTMITFCHHQTIKVLGETIDDAVGEAYDKVAKILGLGYPGGPVIDALSKKGDPRAV
                     LFTRPKLQGRFDTSFSGIKTAVLYLVKDPKTGRPKAHPPSKEDIAASFQHAVIGWLID
                     KVLDAAEDRGVSDIVVGGGVSANSYLRKKLGWEAGARGYQVWFPPISLSTDNGAMIAK
                     RGYELYKKGIRSTLNMTGDPALTINKGNG"
     gene            6036..6470
                     /locus_tag="PK590_03525"
     CDS             6036..6470
                     /locus_tag="PK590_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOG23697.1"
                     /translation="MVIKLGTAVFLGALIGLERGAHGKEAGFRTYALVSLGSALMMLV
                     SVNIFETYKGLSDVDPGRIAGQVVTGIGFLGAGAIIRSQERVRGLTTAAGIWVVCGIG
                     LACGLGAYQEAILGTAFALIILAFFSSVEHKLLRSKDRKPSQ"
     gene            6509..7387
                     /gene="hisG"
                     /locus_tag="PK590_03530"
     CDS             6509..7387
                     /gene="hisG"
                     /locus_tag="PK590_03530"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOG23698.1"
                     /translation="MVSPKLKIAIPKGSLEESTYALFKRAGFTINVNPRSYIPSINDA
                     EIEVILFRPQEMSRYVEDGVVDCGLTGHDWIAENESDVAELCELQYSKRTSNPVRWVL
                     AVHETSPFKSVKDLKGKKIVTELVRVTRRYLKKNKVDAEVEFSWGATEAKPPRLADAI
                     VEATETGSSLRANKLKIIDTVITSIPKFIANKRSVKNSWKKRKMDNLVLLLEGAMRAQ
                     EKVGLKMNLEKKNLAKILSLLPAMKKPTVSNLADENWLGVETIIDDSVVRELIPQLKQ
                     AGASGIIEYPLNKCIP"
     gene            complement(7580..>7856)
                     /locus_tag="PK590_03535"
     CDS             complement(7580..>7856)
                     /locus_tag="PK590_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23699.1"
                     /translation="IGFRNEIVLSGLKSGTVRCQTKIVLSLLDADCVPERDLLENSDE
                     IVITVSASSENSKMKIELSGTLNGQKLHVKRRNAGPREDSPQGSYPL"
BASE COUNT         2067 a         1716 c         2178 g         1895 t
ORIGIN      
        1 gtatcaagtg tgaaaatcaa aaagtggtag aatcggccat atgcttccag aaagcctcca
       61 gggtcgatca actcgaatgg agtgaagcat gattataaaa cggattgccg ttctttccct
      121 gtgtctcatt tttagtcttc ctaatattgc gcgcgcggaa atcccatgga acagcctcaa
      181 tcttcccgcc acgcagtttt ctgaaagctc acgctaccat aaagtgaatt cgactcacga
      241 agatccccac aatccagggt ttctcatttt ttcagtctcc aacgatgcgg ttacggccca
      301 ggtggaaggt ttgttgggtt taaaaaaatg gatccatgaa tcggcggtca tcggacaact
      361 taacgagaat aaaaacgcgg ccgggggtgt ggcggatggc gcgattgaat ccgtcaaggg
      421 gactggccgg gggcttaaaa atctggtcct tcacccgatt gattccgcaa aaggacttgg
      481 ctccgccgcg ggacgggtcg gggacaggat gagcgatgcg tttcgcacaa aagaagaggg
      541 agaaaagggg gacagttttc taagctcctc caaacgccag atcgctcaaa agcttggggt
      601 tgacgtttac tcgcgaaacg aacaactcca ggaaaaactt tcctcattgg ccaaacaggg
      661 aatgggcggg cggagtgtgg tgatggtcgc gaatttcttt ataccattcg gattgattgc
      721 ttcaaccgtt atgaccgtca gcaatatcaa taacgcggcg gaccagttgg tcaatgataa
      781 agacaggatt gatctttaca agatcaacaa agattctctc atgagaatcg gtattcccga
      841 aaacaaggct attgcgcttt tgaacaacac tcattacacg ccccgtcaag ccacctatat
      901 tcgtttttat ctcgaaagat tggctccact tcggggttac cgggtgctgg ctgaccgggc
      961 ggctgaatta accgaagatc tccccgctcg aaagtttctt cacgaaatgc aaatggcggc
     1021 cgacggaatg gcggggatga gcgatgcggt ttcaatcaat atggtaccgg aaggactttt
     1081 actggagaga agggacggcg tggttctgat ttgcggatat gattatctgg cagattcgcc
     1141 actgggcaag acactgatcg aaaaggtcgg tagctataaa gccaaactta aaaagaattc
     1201 cgctgaaata tggaacgcgg gaattgttac gccaaggttg aatggttcgc tcctcgctca
     1261 cggaattaag gtgcgccgga tgtgcctttt tttgaataca tcagatggta atactgcgac
     1321 gtgatgttgg agggagcctt gcgctcaatc gctttttttg ttccgccaac ttatttacca
     1381 atttatttcg atctgaagaa gagttaaaag atgaagaaaa agcgggtttg ccttgtgacg
     1441 ggtggcgccg gatttatcgg ttcccatatt acggatgccc ttctccggaa aggataccgg
     1501 gttcgcgtcc tggataattt ttcacgaggc aagatggaaa acttgagtga ttcgatccgt
     1561 tccatcgacc tggttcgggg tgacatccga tgccttaaaa cggttaaaaa aatgatgaaa
     1621 ggtacggatt acgttttcca tttggccgcg attgcgtcgg tgccccagtc ttgcgaggat
     1681 ccggaggaaa cgcacgaagt gaacgtccga ggcaccctga atgttctgct tgcggcgcgt
     1741 gacgccaaaa taaaacgggt tgtttttact tcttcttcgg caatttacgg agatacgaaa
     1801 aactttccta cttctgaaca ggaacgaccg atgccggaat ctccctacgc ggcctccaaa
     1861 atgatgggag agtattattg ccgcaacttt accgcgcttt atggcttgga aacggtggcg
     1921 cttcgttatt tcaacgtgtt cggcccccgg caggatccga aatcacagta ctccaatgtt
     1981 attccgattt ttatccgtca aatcaaactg gggaaccccg ttatggttca ctgggatggc
     2041 cgacagtccc gtgattttgt ttacatcgag aatgttgtga acgcgaacct gatcgccatg
     2101 gatcatcccc tggccaaggg ggaatcgttc aatgtcggat gccatgagga gaaaaacatt
     2161 ctgggaattg tccgggagct ccggaagtgt atgggaatta aatcgctgaa ggtcaagttt
     2221 tgtcccaagc gcgccggaga tgtgcgccga actttagcgg atatcacaaa agcggaaaaa
     2281 cggatgggat accgtcccac ggtctttttt gacgaaggac tgcgaaagac ggtcgcctgg
     2341 ttcctgaacc gtcctgaagc attgtcccgg tagtttttgt gcgttttgta gggtcaaaga
     2401 cgattttcag gtgtttctga tgagcatcaa tgatctgaat gtggcagaag ttattgcggg
     2461 aaagagttat aaaaatagtt ccgcagttta gcaaaatatc tgttctcaag caaattaagc
     2521 aggaggtggg gtatgcacat caaaaagttc ctgattgctg tgttgttgtt gagcgggtta
     2581 atgtccccgg tttatgcgga gagtgaaaag aaatttactc tcacgactta ttatcctgcg
     2641 ccgtatgggg attataagca acttaaggct actggtccgg atacatctaa tgcaacgata
     2701 gctcttgagg ctcatggcag ttccgggacg gggttggttg tgacgaatgc gaacaatgtt
     2761 ggaatcggga catctgcacc ggctgcaaaa ctggatattg tcgggaatgg cagtactatt
     2821 attgttccac ggaagagtac cgcgggcgat cccgctaatg gtgtcaacgg gatgatttat
     2881 tacaactcaa acaccaataa attccgggcc tatgagaatg gggagtggaa agatttgata
     2941 ggcggaggcg gtaatttgca atgcaaaatg gggagtttct acggaaatgg taaagccgtt
     3001 tggcaaacga taaacgtggg atttagaccg gattttgtgt atatcacagg ttatgctggg
     3061 ggcggcgact gtagcagaga tcgcgtcaaa acaaatacga tgccgggagc gtacgcaaaa
     3121 aagatttatc aaagtagtta tgatgacgac aggatacagt tgacagatac gggttttaaa
     3181 gtgtactacg ctgtgaattg tccaggcgct ccctattttt atgtggcgct taaatccaac
     3241 acatagtttt ttcagaaacc tggtttttga tttttttgtg aggcgtcatc cacaccggtc
     3301 aaaaaagaaa tatgatggat aaagataaaa cgaggtggca gataaaatga attttgaacc
     3361 agtgatcggg cttgaagtcc acgttcagtt aaaaacaaag agcaaacttt tctgcccgtg
     3421 tcctaccact ttcggagaag aggggaaccg aaacacgtgc ccggtgtgtc tggggtggcc
     3481 ggggtcgctt cccgtggtga atgaagaagc gttgaaactc gcgatcaggg cgggtctcgc
     3541 gctccagtgc caggtggctg agaggattaa atttgatcga aaaaattact tttaccctga
     3601 tcttccgaag gcctatcaga tctcgcagta cgacatgccc gtgaacggac ggggttatgt
     3661 ggatgttgaa acaaaaattg acaatgggac ggatttttcg agcggaagcg gagctactac
     3721 gccggagcgg tttgtgacca gaaggatcgg gattacccgc gcgcaccttg aagaagacgc
     3781 gggcaagctg atccatgaag gcgtcgcgga tggcagtctg gttgattaca atcgcggcgg
     3841 tgtgcccctt cttgagattg tttcggagcc tgacatccgg tcgccacggg aggcccacga
     3901 ttatcttaaa gcgctcaaag cgattttgca gtacgcggaa gtgagcgact gtgatatgga
     3961 aaaagggagc ttgagatgtg acgcgaacgt atcggtgcgt cccgtaggcc aggaaaagtt
     4021 tggtacccga gttgagatta agaatctgaa ttcgttcaag atggttgagc gtgccattca
     4081 atacgaaatc gagcgacaga ctcgcgtgct tgaggatggg gagagtattg tgcaagaaac
     4141 gcggctctgg gacgacccga aaggcatgac ctattcgatg cgctccaaag aagacgcgca
     4201 tgattatcgc tatttcccgg acccggatct cgtgccgttt actttggcgc gcgaaaccgt
     4261 ggaggcgatc cgaaaaacct tgccagagct tcctctggac cgcgccaagc ggtttatggc
     4321 ggattatggt cttccagagt acgacgccta cacgctcgtg gaagacaaaa gaatttccga
     4381 atattttgag gacgtggtca aggcgggggt ggcggccaag acttccagca actggattct
     4441 tacggaactg ctcgcggtac tgaatacgaa aaatcttgcg attgaaagtt ctccggtgcc
     4501 gccggaaagt tttgccaggc tactcagact gatcgaagat gggactctct ccgggaaaat
     4561 ggccaaggat gttctcggga tgatgtttga caccggaaag gatgccgaaa caatcgtaaa
     4621 agaaaaggga cttgtccagg tctccgatac cagtcttatt gaaaaagcgg tggagaaagc
     4681 gattgccgca aatcccaggg ccgtggagga gtttaaggcg gggaagcaga aagcgcttgg
     4741 ggcgatcgtg ggttttgtga tgaaagagac acaggggaag gcgaaccccc agcttacgaa
     4801 cgaaatactc aggcgcaagc tcggatagcg gaaaagcgcg gaacaaagaa tgaaaagttc
     4861 gggaaaagca gaacgtagaa aaaaaaagaa gaaacgcgct ccgcttcctg tctcccgctc
     4921 cccggtctac acgcttggcg tagagacatc gtgtgacgaa acctcgtgcg cggttataaa
     4981 aaacggcact gaaatccttt ccaatgttgt ttcctcaagc ctttttcgcc ataaaaaatt
     5041 tgggggagtc gtgcctgaaa tcgcgtcccg gcattccatg gagcaaatcc ggtgcgtctt
     5101 tcatgaggct ctggaagaag cgaagataaa agcttcggat cttgatttga tcgcggtgac
     5161 ccaggggccc gggcttatcg gatcactttt tgtcggggtt tcatttgcta aggcgcttgc
     5221 ctatgaactg gagattcctc tcgtgggggt gaaccacatg gaagcgcacc ttgtttcaaa
     5281 tttttttgaa aaaccggccc caaaaagata cgtcggactt cttgtttccg gcggccacac
     5341 gatgatcacg ttttgtcatc accaaaccat caaagttctt ggcgagacga tcgacgacgc
     5401 ggtgggagaa gcgtatgaca aagtcgcgaa gattctcggt cttggatatc ccggcgggcc
     5461 ggttattgac gctctttcaa aaaaggggga tcccagggct gttctcttca cgcggcccaa
     5521 actccaaggg cgattcgata ccagtttttc cgggatcaaa acagcggttc tctatctggt
     5581 gaaggatccg aagacaggac gtccgaaggc gcacccgcct tcgaaggaag atattgccgc
     5641 gagtttccag cacgcggtca ttggctggct gattgataaa gtgttggacg ccgctgagga
     5701 tagaggagtt tccgatattg tggttggggg aggagttagc gcgaattcct atcttaggaa
     5761 aaagctcgga tgggaggctg gcgctagagg gtatcaagtc tggtttccgc cgatttccct
     5821 ttcgacggac aatggcgcga tgattgccaa acggggatac gagctttata aaaagggaat
     5881 ccggtcgaca ttgaacatga cgggagatcc cgcactgaca ataaacaagg ggaatggtta
     5941 gcggagaatg aataacgatt tcgttctcca tggatcgttt acctgatgga ggaagagttt
     6001 tcatgtgtcc atggacgggt tttcaggatt gggatatggt gatcaaattg ggaacggcgg
     6061 tttttctggg cgctctgatc ggattggaac gaggcgctca cggcaaggag gctggtttca
     6121 ggacgtacgc gctcgtcagt cttggctccg cgcttatgat gctcgtttca gttaacattt
     6181 ttgaaaccta taaaggtctt tccgatgttg atccggggcg aatcgctggt caggtggtaa
     6241 cagggattgg ttttctggga gccggagcga ttatacggtc ccaggagcgt gtccgggggc
     6301 ttacgaccgc ggcggggatc tgggtggttt gcgggattgg acttgcgtgc ggattaggcg
     6361 cctatcaaga agcgattttg ggaacggcat ttgcgttgat aattcttgcg tttttctcaa
     6421 gtgtggagca taaactttta cgctcgaaag accgaaaacc aagccaatag ttgacactat
     6481 ccgagtgagc cgctacaatt tgcgccctat ggtttcccct aaattaaaaa tcgcgattcc
     6541 caaaggtagt ctcgaagaat ccacctacgc cctcttcaaa cgggcgggat ttacgatcaa
     6601 tgtcaatccg agatcgtaca ttccctcgat taatgacgcg gagattgagg tcattctttt
     6661 tcgtccccaa gaaatgtcac ggtatgttga agacggggtg gtcgattgtg gactgaccgg
     6721 ccacgactgg atcgccgaaa acgaatcgga cgtggcggaa ctttgtgaat tgcaatattc
     6781 gaaaagaact tccaatccgg tgcgatgggt gcttgcggtg cacgagacgt cgccctttaa
     6841 aagcgtgaag gatcttaagg gtaaaaaaat cgtgactgag cttgtaagag ttacgagacg
     6901 gtatctgaaa aagaacaagg tcgatgcgga agtggaattt tcctgggggg cgaccgaagc
     6961 caagcctccc cggctcgccg atgccatcgt tgaagcgacc gaaacgggtt ctagccttcg
     7021 cgccaacaag ctgaaaatta ttgataccgt gatcacgtcg ataccgaaat ttatcgccaa
     7081 taagcgaagc gtgaaaaatt cgtggaaaaa acgcaagatg gacaatctgg tgcttttgct
     7141 tgaaggcgcg atgcgggcac aggaaaaagt cgggctcaaa atgaatctcg agaaaaaaaa
     7201 tctggcaaaa atcctttcct tgcttcccgc catgaaaaaa ccgacagttt caaatctggc
     7261 ggatgagaac tggcttggtg tggagacgat catcgacgat tctgtcgttc gtgaattgat
     7321 cccgcagctt aaacaagcgg gagcctctgg aattattgaa tatccgttga ataaatgtat
     7381 cccctaacgg gccgttggaa atggggtttg ggtcggtcgt gacccaggtt ccgtggccgg
     7441 tgatccgtga cagaaggagt gaataatggc ctgggcaaat aaacaacgca agaaaaagat
     7501 gaacaagcac aagcggaaga agaaactccg ccgcaatcgg cataagaaaa aagactggtc
     7561 gtagttttta gcgcagggtt tagagaggat agctcccctg agggctgtcc tctctgggcc
     7621 cggcgtttct acgcttcaca tgcagttttt gaccattcaa agtcccgcta agctcaatct
     7681 tcatcttcga attctccgaa gacgcactga cggttatcac gatctcgtca ctattttcca
     7741 gaagatctct ctcggggaca caatccgcat ccaaaagact caaaacgatt ttcgtctgac
     7801 aacgaactgt cccggacttg agaccggaga gaacaatctc attacgaaag cctatc
//
LOCUS       DAOSIO010000062         7753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_8665_length_7753_cov_4.145492, whole genome shotgun sequence.
ACCESSION   DAOSIO010000062 DAOSIO010000000
VERSION     DAOSIO010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 7753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7753
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8665_length_7753_cov_4.145492"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..576)
                     /locus_tag="PK590_03540"
     CDS             complement(154..576)
                     /locus_tag="PK590_03540"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOG23700.1"
                     /translation="MSWDERGLSLLEILVASVILGVALLGFTSSLQFIEKTHTVRERQ
                     LEAQGYARETLESLLLSTRDYNATELTDGNHTSKNTEDPDLTLPSNIGLQGASRFYTV
                     TAYDKDGVTGKRIDVTFSWNEGGETRQEQLRGFLLNPT"
     gene            complement(573..983)
                     /locus_tag="PK590_03545"
     CDS             complement(573..983)
                     /locus_tag="PK590_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23701.1"
                     /translation="MRNTKLKETRGAALVTVVVVIIAVAMLVAAGLNFVTGASSLSKE
                     SLDGVRAMYLAEAGLQRALHEIGTKDDPATEEEEPLEWEFLKDKIRITIVASSGDPNL
                     YDVTSTVLVGRAERMSTATIRKQGGKAVMTGWSL"
     gene            complement(1103..2842)
                     /locus_tag="PK590_03550"
     CDS             complement(1103..2842)
                     /locus_tag="PK590_03550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG23702.1"
                     /translation="MEEQEPFDSTEKLFTRTRSRRSLDWLRVFIFSLITGLFVIFFMS
                     GTLTRLENMVLDIFHRYGGLTQTDPRIMLIEIDEESIDEIGPWPWPRRYHAVMARLLD
                     QWGAAAILFDFNFPGETDPKDDQDLVRVLESIRAPFYLPVDYRPQKDKKSWLHGLPVV
                     FEKNEGKMVWDHSLQSIEKNARGLGHHFFKNDPDGILRGIDLRLSDGVEEFPYLGILP
                     ATVFSGDPSREMTELKSVEDENGRVLIPWLRDRGSQFSKCNFAEIIHSYYGIRKGMAP
                     ALSPELIRGKICLVGLTSQTQAPVTALMTHAQMMNAALTGQWIRPVSRFSNLIFLLLV
                     GLMAGLLFMVLRGAMSLLAGLFLAAGWVTFAFVAFKYLHLWIFSAHQLLLILALFVFS
                     AIYVQVTASRDRSVLFHLATRDGLTGLYVIRHFRVIMNQIVREATFRKEPLSIILIDI
                     DHFKNINDTHGHPAGDMILKKVAHTLAAHIRKKRPFSQVDFAARYGGEEFIIMLRKSG
                     LKEAVEQVAERLREKVANTQFEWEGKSVQVTISLGVATLRPGENVPDPMVRRADAALY
                     RAKARGRNRVCSE"
     gene            complement(2863..4254)
                     /gene="mnmE"
                     /locus_tag="PK590_03555"
     CDS             complement(2863..4254)
                     /gene="mnmE"
                     /locus_tag="PK590_03555"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOG23703.1"
                     /translation="MAKLELDDTIAAIATPLGEGGISVIRLSGPGAFDCLRPFFLSTS
                     RKSPDTFPPNTIHWGRVVDSEGKLIDQVLLSVFRSPQSYTGQDVLEVSCHGGLAVTKR
                     ILGLFLSNGARHAEPGEFTRRAFLNGKIDLSQAEAVLDLIHAKSNRSLDIAIRQLTGE
                     LSKRFQGLKDLILRLLAHMEAYIDFPDENLEVDVSSGMERQFSELLKTIRALLEGFDR
                     NQLVREGALVTIVGKPNAGKSSLFNALLERDRALVSELPHTTRDHLEESLEIKGFYVR
                     LRDTAGLVSTPEQALDKLGMDRTLRALKESHLVLFLTDGSRPLDDSDRHVFKQIPDGK
                     KVIILINKSDLPEKLERTELARLTGRTDPVGISTKTREGLKEIERQIAAFLEQDMAGE
                     GEQVTRLRHKRALEKALELIARAELSFREKERFELITIDLRAALEIMKELTGEVHSEE
                     LLDMIFSEFCIGK"
     gene            complement(4317..5570)
                     /locus_tag="PK590_03560"
     CDS             complement(4317..5570)
                     /locus_tag="PK590_03560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOG23704.1"
                     /translation="MNYSQSLDYLNGFLNLEKLAEYPDNSFFNLRRMEHLLRAAGRPD
                     QNFLPVLVAGTTGKGSTGFFLESILKENRISVGYYHSPHVETVRERIRLGGALVSRAL
                     WAEGLSDVQKLLTQNPLPPRLGVLTYFEVMTFLAIWLFSKTRRKVGIFEIGLGGRLDA
                     TNVLRSPVVILTPIHLDHENFLGNTIAKIAREKAGIIKKNCFVISGEQRPEAERVIRR
                     QVRAMGAHQIKSTPYRGNKTGLAGDFQKTNAGTAIQAARVLRLAFGFPVNPESYSRAV
                     KKQNWSGRMERLHAFRREFILDGAHNPVSVQGMVKAAGGLCRPRETWVIFGAMQDKNS
                     EVMLRVLSRRFRKIVLTKIQNPRARSVGVLLEQAKGLFKIAIPAQNIREAISRAREGS
                     RAGSSILVTGSFYLVGEARKLLRKA"
     gene            complement(5650..7224)
                     /gene="purH"
                     /locus_tag="PK590_03565"
     CDS             complement(5650..7224)
                     /gene="purH"
                     /locus_tag="PK590_03565"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004697992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOG23705.1"
                     /translation="MSRQVIRRALISVSDKNGLIPFARTLHKHRVEILSTGGTLKALR
                     EARVPARAVDEFTGFPEMLDGRVKTLHPKIHGGLLYLRSKKSHCQQARQHGIQPIDLV
                     VVNLYPFESATAKGNLKLHEAIEFIDIGGPSMLRSAAKNNESVTVVTDPADYARVMEE
                     MKKNRGSVTRELRFELAQKVFERTSSYDAVISQYLAAQKRQTEENTLPVHFGSCFKKA
                     QDLRYGENPHQKAAFYSYATRKASVNWKQLHGKELSYNNLLDIEATMDVLAEFDAPAA
                     CVIKHNNPCGIASAPDLVKAVEDAIDSDPLSAFGGIVGLNRACDPATAQRILGKLGFF
                     EVLVAPAFKPGALKILKTRKNLRLMEVDSFQETYPYEFKFLRSGVLVQEKNRPIKDHM
                     GKLKKNLRFVTRAKLNKKEMDDLVFAFQCSKVVKSNAIILTQGQRTVGIGAGQMSRVD
                     SMQIAISKAGYLAEGAYVGSDAFFPMPDSIELAAKHKIRAIIQPGGSIRDSEVIAACD
                     AAGIAMVFTGERHFRH"
BASE COUNT         1877 a         2181 c         1874 g         1821 t
ORIGIN      
        1 aaattaaaac gaaccgtggc cgtcatcagc aaaacgccgc ccagtagcgc caacgccacg
       61 atcaactcaa aaagactcag acccctctgc cgtacctttc gcttcatcct ccgcatctca
      121 ctcctttctc caaatggtaa cacatccaga gcgtcacgtc gggttcagca gaaaacccct
      181 caattgctcc tgtctggtct caccaccttc attccatgaa aacgtcacat caatccgctt
      241 gccggttact ccatccttat cgtaagccgt caccgtataa aaacgactgg ctccctgtaa
      301 accaatattg gacggcaagg tcagatccgg atcttcggta tttttacttg tgtgattccc
      361 gtcagtcagt tcagtggcat tataatcacg cgtcgaaaga agtaacgact ccaaagtttc
      421 acgcgcgtaa ccctgggcct caagctgacg ctccctgacc gtatgggtct tttcaataaa
      481 ctgaaggctc gaggtaaaac caagcaatgc cactccaaga atgacactgg ccaccaaaat
      541 ctccagaaga ctcaatcccc gttcatccca gctcataagc tccaccctgt catgactgcc
      601 tttcctccct gttttctgat cgtcgccgtg gacattcgct cggcacgacc caccaaaacc
      661 gtggatgtga catcgtacaa gtttggatca ccactactgg cgacaatagt aattcgaatt
      721 ttatctttta aaaattccca ttcgagaggc tcttcttctt cagtagcggg atcatctttg
      781 gttccgattt catgcaatgc ccgctgaaga ccggcttcgg caagatacat ggcgcgaacc
      841 ccatccaggg attccttact caaggatgac gcgccagtca caaagttcag acccgccgcc
      901 accagcatcg ccaccgcaat aatcaccacg accaccgtca cgagcgccgc gccacgtgtt
      961 tctttcagtt ttgtgttcct catcgcgttc tccgtttctt tgtcaaaatt acggcaaaac
     1021 tctaaataaa tagagccaat tccaaaaata cttcgctttc caatccttta attcttaaac
     1081 tcctgaaaaa taaaaaatga aatcattcgg aacaaaccct gtttcgtccc cgggccttcg
     1141 cgcgatacag ggccgcgtca gcgcggcgaa ccatggggtc gggaacattt tccccgggac
     1201 gaagcgttgc cacgcccaga ctgatcgtca cctggacaga ttttccttcc cactcaaatt
     1261 gggtattcgc cactttttct ctcagacgtt ccgcgacctg ttcgaccgcc tctttcaacc
     1321 ctgactttcg aagcataata atgaattctt ccccgccata acgggccgcg aaatccacct
     1381 gggagaacgg ccttttcttc cggatgtgcg cggccagcgt atgagccact ttctttagaa
     1441 tcatgtcccc cgcgggatgt ccgtgagtat cgttaatgtt tttaaagtga tcgatatcaa
     1501 tcaaaataat cgacaaaggc tctttgcgaa atgtcgcttc ccggacaatc tggttcataa
     1561 tcacccggaa atgacggatc acataaagcc cggtcaaacc gtcccgtgtc gccagatgaa
     1621 aaaggaccga ccggtcccgg gacgccgtaa cctgaacata aatcgccgaa aacacaaaga
     1681 gggccaaaat cagcaggagc tgatgcgccg aaaaaatcca gagatgcaga tatttaaacg
     1741 ccacaaaagc aaaagtcacc catcccgccg ccaaaaagag acccgcgaga agcgacatgg
     1801 cccctcgcaa aaccataaaa agcagaccag ccatcagacc gaccagcaac agaaaaatca
     1861 gattggaaaa ccgggagacg ggacgaatcc attgtccggt caaggcggca ttcatcattt
     1921 gcgcgtgagt catcagggct gtcacgggcg cctgggtctg ggaggtaaga cccacgaggc
     1981 aaatttttcc ccggatcagt tcgggcgaca aggccggggc catgcccttt cgaattccgt
     2041 aatagctgtg gatgatttcg gcaaaattac atttcgaaaa ttgacttccg cggtccctga
     2101 gccaggggat gagcacgcgc ccattttcat cttccaccga tttaagttcc gtcatctcac
     2161 gggacgggtc gcctgaaaaa actgtggccg gcaaaatacc aagataagga aactcctcga
     2221 ccccgtcgct caaccggaga tcgatcccgc gcaaaatccc gtccggatca tttttgaaaa
     2281 aatgatgtcc aagcccccgc gcgttttttt cgatgctttg aagcgagtgg tcccagacca
     2341 tttttccctc gtttttttca aacacaacgg gaagcccgtg aagccaggat tttttatctt
     2401 tttgagggcg gtaatccacc ggcagataaa aaggggcgcg aatactttcc aggacgcgga
     2461 caagatcctg atcgtctttc ggatcggtct cgccggggaa attgaaatca aacaggatcg
     2521 cggcggcgcc ccactggtca aggagcctgg ccatcaccgc gtgataccgc cggggccagg
     2581 gccagggccc gatctcgtct atcgactctt catcaatttc gatcagcatg atccggggat
     2641 ccgtttgcgt caaacccccg tagcggtgaa aaatgtccag gaccatattt tccaggcggg
     2701 tcagggtccc ggacataaaa aaaatcacga aaagtcccgt gatgagactg aaaataaaaa
     2761 ctctgagcca gtcgagagac cggcgggacc gggtcctcgt aaaaagtttt tccgtgctat
     2821 caaacggttc ttgttcttcc atcgattccc ttccgcgccc ggttatttcc cgatgcaaaa
     2881 ttccgaaaag atcatgtcca gcaattcttc cgagtgaact tcccctgtca actctttcat
     2941 gatttcaagc gccgcccgaa ggtcgatcgt gataagctca aacctctctt tttccctgaa
     3001 ggataactcg gcccgcgcaa ttagttcaag cgccttctct agcgcgcgct tatgcctgag
     3061 ccgtgtcacc tgctccccct ctccagccat gtcttgttcg aggaaagcgg caatctgccg
     3121 ttcaatttct tttaagcctt ccctagtctt cgtggaaatt ccgaccggat ccgtccttcc
     3181 cgtcaggcga gccaactcgg tccgctccag tttttccgga aggtcagatt tgttgatcaa
     3241 aatgataact tttttcccgt ccggtatctg tttaaatacg tgtcggtccg aatcgtccag
     3301 gggccgcgaa ccgtcggtca gaaacaggac cagatgactt tccttgaggg cccgcaaagt
     3361 tcgatccata ccaagtttgt ccagcgcctg ctccggcgtc gagacaagtc ccgccgtgtc
     3421 ccggagccgg acataaaaac ccttaatctc cagggattcc tcaaggtggt cgcgggtcgt
     3481 gtgaggaagt tcggaaacca gcgcccggtc gcgttcaagg agcgcgttaa agagagagga
     3541 tttaccggca ttgggttttc cgacaatcgt aacgagagcg ccttcccgca caagctggtt
     3601 acgatcaaat ccctcaagaa gcgcccgtat cgttttgaga agttctgaaa attgtcgttc
     3661 catgccggag ctgacatcca cctccagatt ctcgtcggga aaatcgatat aggcttccat
     3721 gtgggccaag agtctcagga tcaggtcctt taatccttga aaccttttcg aaagttctcc
     3781 cgtcagttgc cggatcgcga tatcaagcga gcggttactc ttggcgtgga taagatcaag
     3841 gacagcctcc gcctgcgaaa gatcgatctt gccgttcagg aaagcccgtc gcgtaaactc
     3901 acccggttcg gcgtgccggg cgccgtttga aagaaaaagt ccgagaatcc tttttgtgac
     3961 cgcgagcccg ccatgacagc taacctcgag aacatcctgc ccggtatatg attgaggcga
     4021 gcgaaaaacg ctgaggagga cctggtctat caactttcct tcggagtcaa ccacccttcc
     4081 ccagtgaata gtattgggcg gaaaggtatc gggtgacttc cggctggttg acagaaaaaa
     4141 aggccggaga cagtcaaaag ctcccgggcc gctgaggcgt atcaccgaaa tcccgccctc
     4201 tccgagtggc gtcgcaatcg ccgcaatagt gtcatcaagt tcgagctttg ccatttgacc
     4261 cccggctcaa gataaaaagc gacaagttcc tgacttcgcg ccccgcgcgt taaacgttat
     4321 gctttcctta aaagttttct tgcttctcca accagataaa aagaacccgt aacgagaatg
     4381 gaagacccag cacggcttcc ttcccgggcc cgcgaaatcg cctcccggat attctgggcc
     4441 gggatggcga ttttgaaaag ccctttcgct tgctcaagca aaaccccgac cgatctggcc
     4501 cgcggatttt gaatttttgt caggacgatt ttccgaaacc gccggctcaa aacgcgtaac
     4561 ataacttctg aatttttgtc ctgcatcgcc ccaaaaataa cccaggtttc ccgggggcga
     4621 caaagaccgc cggccgcttt caccattcct tgtaccgaaa cgggattatg agcgccgtcc
     4681 agaataaact ccctccgaaa ggcgtgaaga cgctccatgc gtcccgacca gttttgtttt
     4741 tttaccgccc gggagtagct ttcagggtta accggaaaac caaaagccag ccggagaact
     4801 cgcgcggcct ggatcgcggt ccccgcattt gttttttgaa aatcacccgc gagtccagtt
     4861 ttgtttcccc ggtacggcgt cgatttgatc tggtgagctc ccatggcccg aacctgccga
     4921 cggatgacac gctccgcttc gggccgttgc tcaccgctga tgacgaaaca gttttttttg
     4981 atgatccccg ccttttcgcg ggcaatcttc gcgatcgtgt ttcccaaaaa attttcatgg
     5041 tccagatgga tcggcgtcag gatcaccacg ggcgaacgaa ggacattggt cgcgtcaagc
     5101 cgtcctccga gaccgatttc aaaaatcccg acttttctcc gggtctttga gaaaagccat
     5161 atcgccagaa aggtcataac ctcaaaataa gtcaggaccc caagccgcgg aggtaaagga
     5221 ttttgggtga gtaatttttg aacgtccgaa agtccctcgg cccagagagc ccgggaaacc
     5281 agcgcaccgc caaggcgaat cctttcgcgg accgtttcaa cgtgcggaga gtgataatat
     5341 ccgacggaaa tccggttttc cttcaggatc gattccaaaa aaaaacccgt ggagcctttt
     5401 ccggtcgtcc cggcaacgag cacagggaga aagttttgat cgggccggcc cgccgcacga
     5461 agaagatgtt ccatgcgtct caggttgaaa aaagagttgt caggatattc ggcgagcttc
     5521 tcgagattta aaaaaccgtt aagatagtcc aggctttgag agtagttcat cgtcgcgagc
     5581 accgcgtgag gcgcgccgcg tcagggaaca acttttccgt ttcccgtcaa gcgtcgcgcc
     5641 cggcgtgagt tagtgtctga aatgcctttc cccggtaaag accatggcga tccctgccgc
     5701 gtcacaggcc gcgatcactt cagaatccct gatggatccg ccgggctgga taatggccct
     5761 gattttgtgt ttggccgcaa gttcgattga atccggcatc gggaaaaagg catccgaacc
     5821 gacataagcc ccttcagcaa gatacccggc ttttgaaatc gcgatctgca tggaatccac
     5881 ccgggacatc tgtccggcgc cgatccccac ggtccgctgg ccctgggtca ggatgatcgc
     5941 gttggacttc acgactttcg agcactgaaa cgcaaaaaca aggtcgtcca tttctttttt
     6001 attcagcttc gcccgggtga caaaacggag attcttttta agtttgccca tgtggtcctt
     6061 gatgggacgg ttcttttcct ggacaagaac accgcttcgc aggaatttaa attcgtaagg
     6121 ataagtttcc tgaaaactgt ccacttccat gagcctcagg tttttacgcg ttttcaaaat
     6181 ctttaacgcc cccggcttga aggccggcgc caccagcact tcaaaaaaac ccagcttccc
     6241 gagaatcctt tgagccgtcg cggggtcaca ggcgcgattc aaccccacga tccccccgaa
     6301 ggccgaaagg ggatccgaat caatcgcgtc ttcaaccgcc ttgacgagat ccggcgcgga
     6361 agcgattccg cagggattgt tgtgtttgat cacgcaggcg gcgggcgcgt caaactccgc
     6421 cagcacatcc atggtggctt cgatatcaag caggttgttg taggaaagct ccttcccgtg
     6481 gagctgtttc cagttcactg acgctttgcg ggtggcgtac gagtaaaagg ccgctttctg
     6541 atgggggttt tccccataac gaaggtcttg agcttttttg aaacaggaac cgaaatgaac
     6601 cggaagcgtg ttttcttccg tttgtctttt ttgggccgcc agatactgac tgatcaccgc
     6661 gtcgtacgac gaggtccgtt caaaaacttt ttgagccagt tcaaagcgga gctctcgcgt
     6721 aacggatccg cggttttttt tcatttcttc catcacccgg gcataatccg ccggatcagt
     6781 cacaacggtc accgactcgt tatttttggc cgctgatcgg agcatggaag ggcccccgat
     6841 atcaataaat tctatcgctt catgaagctt gaggtttccc tttgccgttg ccgattcgaa
     6901 aggataaaga ttcacgacca cgaggtcaat cggctgaatt ccgtgttgcc gggcctgctg
     6961 acagtggctt tttttcgagc gaagataaag aagccctccg tgaatcttgg gatgaagggt
     7021 cttgacgcgg ccatcaagca tttcgggaaa tccggtaaat tcgtcaaccg ccctggccgg
     7081 cacacgggct tcccgaagcg ctttaagagt cccgcccgtg gagaggattt cgacgcggtg
     7141 cttgtgcagg gtcctggcga atggaataag cccgttttta tcggaaacgc tgatcagcgc
     7201 tcggcggatg acttgtctgg acataatacc tgaatcctcc tctttaaaaa gcggtgaagc
     7261 cgcgatgtga cggggccggg aacctccctg ccgatctttc gtccgtccag ttccctaacc
     7321 ggaagtattt cccaggaagt gttagaaagg aaagcttcgt ccgcgttgaa aaaatcgtgt
     7381 cgtgtaagat tcgactctct gaccttaagt ccgagatcag ttgcgcattt tatcacaaag
     7441 cgccgggtca ctccattcaa gattaggcgc tcaataagac agctttattc tgtcctcttc
     7501 ctaccagaaa atctccggca aaaacagacc ttttgccttt ttctgactta gcagacttta
     7561 gaggatcgat ctcttttccg gggaaaaccc gatataggaa tactttgggt cgggaggggt
     7621 cttgtgatgg ctgtcgagga aaaaacaaac ctggtattca cgttcaatgt tctgaaacac
     7681 ttcccatgga gtgagggacg accggaactg tcggatatga agaggcatgg cttctttgct
     7741 catccgtcac cgg
//
LOCUS       DAOSIO010000063         7594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_8905_length_7594_cov_4.066322, whole genome shotgun sequence.
ACCESSION   DAOSIO010000063 DAOSIO010000000
VERSION     DAOSIO010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 7594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7594
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8905_length_7594_cov_4.066322"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PK590_03570"
     CDS             <1..406
                     /locus_tag="PK590_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012575333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG23706.1"
                     /translation="NLKSAFLFTKACARPMMKQRSGAIVNIASIVGIAGNAGQANYSA
                     SKGGMIALTRTTAKELAKRNIRANAIAPGFIKTKMTDKLSPELIEKMKEHIGLGRLGE
                     PQDIANVAVFLVSDASSYVTGQTIVVDGGLVI"
     gene            452..691
                     /locus_tag="PK590_03575"
     CDS             452..691
                     /locus_tag="PK590_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007545510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOG23707.1"
                     /translation="MGVQEKITEIIVEQLGVKVEEVVPEASFVDDLGADSLDTVELVM
                     AFEEEFGTEIPDEDAEKIQTVGDAIKYIEEKSGQK"
     gene            748..1989
                     /gene="fabF"
                     /locus_tag="PK590_03580"
     CDS             748..1989
                     /gene="fabF"
                     /locus_tag="PK590_03580"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOG23708.1"
                     /translation="MRRRVVVTGVGAVTPLANNAEDTWQGFLAGKTGTGHLTQIDPAP
                     FNSKVSGEVKGFDPTQYQTLKDTKKTDRFVQFGIASAKMALENSKLNLSNENVERCGI
                     LVGSGIGGLRVIEEQHKVLMEKGAGRISPFLIPMLIVNMAPGQISIALGLKGPSTCVA
                     TACATGSNAIGDAFKIVQRGEADIMFAGGTEACITSLGFGGFDAMKALSTLNEDPSTA
                     SRPFDASRAGFVMGEGCGVIILEELEHAKKRNAPILAEFVGYGMTSDANHITAPDPSG
                     EGAARCMLNAMKEAGIRPDEVDYINAHGTSTYLNDKVETIAIKKALGDHAYKTRVSST
                     KSMTGHLLGAAGAVEAVACILAIRDQIVPPTINYKNPDPDCDLNYVPNQAEKTKVRVT
                     LSNSLGFGGHNACIAFKAFEE"
     gene            2084..5737
                     /gene="smc"
                     /locus_tag="PK590_03585"
     CDS             2084..5737
                     /gene="smc"
                     /locus_tag="PK590_03585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOG23709.1"
                     /translation="MRLKKLEIVGFKSFADKTDIFFDKGVTCIVGPNGCGKSNVSDSI
                     RWVLGERSAKILRGSSMEDVIFNGTEVRKPLAFAEVSLTIDNSDHTLPIEYNDVTIAR
                     RLYRTGESEYLLNKTVCRLKDLQDLILDTGIGSSSYSMIEQGRIDYILKADPEERRFL
                     IEEAAGIAKFKAKKDESIRKLERTEDNLKRLNDIVLEVQKNIQYAERQAKRAEKYKVE
                     LERLKSLEIKRAFLFQGQIATQTSQLNEKIQFQKNELGRLETVLSESQAQLAQQEILL
                     RDVLDRYQQKESERYGVLSKIETNEQKSQFLREKKIEIAQQRGQVLQEQDQVNQSLGA
                     GQQKKEDMEKLLKTQEADLSSCQDRLTQVQTTLNAHESSIQACKEVLEQCRHDFLEAS
                     QATIHTRNEYQRYRSLKENLDQQCKRGESETSRVSAEIAVWDQRLTNVTQLIETNEAE
                     YRSLEQKLSLIETQLSEQTRTRTAQEEKIRQIQLSIKEKDSRLRVLTEVDEHQETSFH
                     DFIAENEAEQQGLVRDLKTVLKVAEGKEWALDIALGHFVRSVIVQDLPTAKTLAEKLK
                     ARRSFTTGILLQNFLPSFASLPDAEAMYRSAGFSPLLSFVTPASGWEEAVSGILGNIY
                     VAEQFPFQFLEAFLQGDTRALLITQDGFLFTPDKKIFFWNNTNRMEFAFSRSGEVARI
                     RQEIDQLRLDMTSAEGNLEGVASGQRNLLTEAESSRTKKTELLIARESLETEKKNIKE
                     RLAAFQQEMDLTQSELQDMVRESQEIFGKETHLEAELRQKEAEESRVHGIQMEKENEL
                     QSLLSERENVLLEVQQVRSQTEQYQQGIHHLQDSMRLIAQHIAKDNDRLVFLGEGLAR
                     FDAKDVELAEDESRCHGMAQEYQSQLREVDLSLELLRKEKTEAETRLEQQNQAIHASQ
                     EKQKQIQEAQHQISLQIMDLDYQFKNVAERLLQRYRLNLADLDPQAYPFTPEDLQNVE
                     EDIAQLQTKVDSLGTVNLLAIEEYQELKQRYDFLVAQKKDLEDARDSLMEAIRKINRT
                     TKQLFEDTFAQVQVTFREYYQTLFRGGDARLTLIDEEHPLDSGIDIVVRPPGKKLQNM
                     SLLSGGEKALTAVALLFALFKIRPSPFCLLDEVDAPLDEANIDRFLAVLRSFVDTTQF
                     LIITHNRKTIAMGDSLYGVTMEEPGVSKIVSVKVRSGDESVPGEDLHPSVATEEAMRA
                     IQHPQETTGAVTS"
     gene            5734..6273
                     /locus_tag="PK590_03590"
     CDS             5734..6273
                     /locus_tag="PK590_03590"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOG23710.1"
                     /translation="MSIPNYLTLLRIILTPVFITSLISYSPEKEYLRYWALAIFALAG
                     LTDALDGILARVLRQKTALGEILDPLADKLLLLSGYISLLFVDSVLYHPPLWITIVII
                     FRDVVLSIGFFTLHFLRVPFDAKPNLIGKATTVFQMSLLLLILLKWPVAVPVAYVTGF
                     LTILSGVIYVSKALKRIPS"
     gene            6270..6869
                     /gene="plsY"
                     /locus_tag="PK590_03595"
     CDS             6270..6869
                     /gene="plsY"
                     /locus_tag="PK590_03595"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOG23711.1"
                     /translation="MIVLFFLAAYLLGSIPFGLMAGYFLKGIDIREHGSKNIGATNVF
                     RTVGKKWGITVLFLDALKGWIACLLPHCFGIADLPTQITLLLGVAAILGHTFPVWLKF
                     KGGKGVATSLGVFLAICWEATVATFGIWILALLITRIISMASIVAVLTFPVMVAFFYW
                     GNPDVYWLLPISIFLGAFILYTHRSNISRLRQGIEKKIF"
     gene            7107..>7594
                     /locus_tag="PK590_03600"
     CDS             7107..>7594
                     /locus_tag="PK590_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23712.1"
                     /translation="MATERKKTEKPASDPKKTTGDKEKALELALSQIEKQFGKGAIMK
                     LGDSRHENVVPIPTGALSLDIATGIGGIPRGRIVEIYGPESSGKTTLTLNVISRIQSQ
                     GGVAAFIDAEHALDPVYAKKIGVKVDDLLISQPDSGEQGLQIAEMLVRSNAVDLIVVD
                     SVA"
BASE COUNT         1992 a         1731 c         1951 g         1920 t
ORIGIN      
        1 caatctgaag agcgcgtttc tctttaccaa ggcgtgcgcc cggcccatga tgaagcagcg
       61 gtccggggcg atcgtcaata tcgcctctat cgttgggatt gccggaaacg ccggacaagc
      121 gaattattcg gcctctaaag gtggaatgat cgcgctgacg aggacgacgg cgaaggaatt
      181 agccaaacgt aatatacgcg ccaacgcgat tgcgcccggc ttcatcaaga ccaagatgac
      241 ggacaagctt tcgcctgagt tgatagagaa gatgaaggag catatcgggt taggacggct
      301 tggggaacct caagatattg cgaatgttgc cgtattttta gtttcagatg catcatcgta
      361 cgttacagga caaacgatag tagtggacgg cggccttgta atttgacagg cctttcaagc
      421 aacaaggtcc caaatcatta aaggaggaat catgggagtc caggaaaaaa tcacagaaat
      481 tattgtcgaa cagcttggcg taaaagtcga agaggttgtt cccgaagcgt ctttcgtgga
      541 tgatcttgga gccgattcgc ttgatacggt tgagcttgtg atggcgtttg aagaagagtt
      601 tggaaccgag attcctgacg aagacgccga aaagattcaa acggtcggtg atgccatcaa
      661 atatatcgaa gaaaaatcgg gacagaaata agcgttccgt ttaccttcct acgttttagc
      721 aagtaccata ccaattaagg aacccatatg aggcgtcgtg tagttgtgac gggtgttggc
      781 gcggttactc ctcttgccaa caacgccgaa gatacctggc agggttttct tgccgggaaa
      841 accgggacgg gtcatcttac tcagattgac ccggcccctt ttaactcgaa agtttccggc
      901 gaggttaagg gttttgatcc cacccagtat caaacactca aagacacaaa gaagacggat
      961 cgatttgtgc agtttgggat tgcgagcgca aaaatggctc ttgaaaattc caagctgaat
     1021 ctgtcaaacg aaaatgtcga gcgatgtgga attctcgttg gttccggaat tggcggtctt
     1081 cgtgtgattg aggaacagca caaggttttg atggagaaag gggcgggacg aatttctccc
     1141 ttcctgattc ccatgttaat tgtgaatatg gcgcccggac aaatttcgat cgctttgggt
     1201 ttgaaagggc cttccacctg tgtcgcgacc gcctgcgcca cgggatcgaa cgcgatcggg
     1261 gatgccttca agattgttca gcggggcgaa gccgatatta tgtttgccgg agggacggaa
     1321 gcctgtatca cgtcgctcgg gtttggaggc tttgacgcca tgaaagcctt gagtacgtta
     1381 aacgaagatc cttcgacggc gtctcgtccg tttgacgcga gtcgcgccgg atttgtgatg
     1441 ggcgagggtt gtggtgtgat tatcttggag gagctcgaac acgctaaaaa acgcaacgct
     1501 ccaattctcg cggagttcgt tggatatggc atgacgtcgg acgcgaatca tatcacagcg
     1561 ccggatcctt ccggggaggg tgccgcgcga tgcatgctta acgcgatgaa agaagccggc
     1621 atccggcccg atgaggtcga ttacatcaat gcccacggaa cgtcgactta cctcaatgat
     1681 aaggttgaga cgattgcgat taaaaaggcg ctgggggatc atgcttataa aaccagggtt
     1741 agctctacaa agtcaatgac cggacacctc ctcggagcgg ccggagcggt tgaagcggtc
     1801 gcctgtatac tcgccattcg ggatcagatt gtacctccca ccatcaatta caaaaacccg
     1861 gatcctgact gcgatctgaa ctacgtccct aatcaggcag aaaaaacgaa ggttcgcgtc
     1921 actttgtcca attcgctagg tttcggaggc cataatgcct gtatcgcatt caaggccttt
     1981 gaagagtaat tttctgaaat ttccttcttt agacattccc cctgtctgcc gatcattatc
     2041 aagaagattt ttttagcaaa aatgactctg gaagagatga accatgcgtc ttaaaaaact
     2101 tgaaatcgta ggctttaaat cgttcgcgga caaaaccgat attttttttg acaaaggcgt
     2161 gacgtgtatc gtcggtccga atggatgtgg gaagagcaat gtctcggatt cgatccgctg
     2221 ggtgctgggc gagcgtagcg ctaaaattct ccgcggttct tccatggaag atgtcatctt
     2281 caacgggact gaagtccgca agcctcttgc gtttgccgaa gtctctctta cgatcgataa
     2341 ctcggatcat acgttgccga ttgaatataa cgatgtgacg atcgcccgcc gtctttaccg
     2401 aacgggggaa agcgaatatc ttctcaacaa gaccgtttgt cggctcaagg atttgcaaga
     2461 tctcattctt gatacgggca tcggttcttc ctcttactcc atgattgaac aggggcggat
     2521 tgattacatt ctcaaagcgg atcctgagga acgtcggttt ctcatcgagg aagcggcggg
     2581 gatcgcgaaa ttcaaggcga aaaaagacga atcgatccga aaactggagc gaaccgagga
     2641 taacctcaag cgcttgaacg atattgttct cgaagttcag aaaaatattc aatatgcgga
     2701 acgccaggcc aagcgcgcgg aaaaatacaa ggtcgaactc gaacgcctca agtcgctgga
     2761 aattaaaaga gcctttttgt ttcaagggca aattgcgacg caaacttccc agcttaacga
     2821 aaaaattcag tttcaaaaaa atgagctggg tcgacttgag actgtgctat cagagtccca
     2881 agcgcaactg gcccagcagg agattttgct cagggatgtt ctggatcgct atcagcaaaa
     2941 agaatcggag cgatacgggg ttctgtccaa gatcgaaacc aatgaacaaa aatcccagtt
     3001 tttgcgtgag aaaaaaattg aaatcgccca acaacggggc caggtcctgc aggagcagga
     3061 tcaagtaaat caatcccttg gcgccgggca acaaaaaaaa gaagatatgg agaagttact
     3121 taaaacccag gaagcggatc tgtcatcgtg tcaggatcgc ctcactcagg tgcaaacgac
     3181 actcaacgct catgagtctt cgatccaggc gtgcaaggaa gttttagaac aatgccggca
     3241 tgattttttg gaggcttccc aggcgaccat tcacacacgt aacgaatatc aacgctaccg
     3301 gtctctcaag gaaaatctcg atcagcagtg caaacgggga gaatcggaaa cttctcgcgt
     3361 ttcagccgag attgcggttt gggatcaacg acttacgaat gtaacgcagt tgatcgaaac
     3421 caacgaagcc gagtaccgct cgcttgaaca aaagctttcc ttgatagaaa cgcaacttag
     3481 cgaacagact cgtacccgca cggcacaaga ggaaaaaatc cggcaaattc aactttcgat
     3541 caaggaaaaa gactcccggc tccgggttct cacggaggtt gatgaacatc aggagacttc
     3601 gtttcacgac tttatcgcgg aaaatgaagc ggagcaacaa ggcttggtgc gcgaccttaa
     3661 aacggtgctc aaggtcgctg aagggaagga gtgggctctg gatatcgcgc tgggacattt
     3721 tgtccggtcc gtcattgtcc aggatttgcc gaccgcgaaa actttggcgg aaaagttaaa
     3781 agccaggcgt tcgttcacga ctggaatcct tcttcaaaac tttttacctt ctttcgcgag
     3841 tcttccggac gcggaggcaa tgtatcggtc agccggtttt tcacctttgc tctcgtttgt
     3901 gacccccgcc agcggttggg aagaagccgt ttctggaatt ctcggtaata tttatgttgc
     3961 tgaacaattc ccgtttcagt ttttggaagc atttttgcag ggagatacgc gcgcgcttct
     4021 gattacccag gacggttttc tttttacgcc cgataaaaaa atctttttct ggaataatac
     4081 caatcgcatg gagttcgcct tttcccgaag cggcgaggta gcacggatcc gccaggagat
     4141 tgaccaactc cggttggata tgacttccgc ggaagggaat cttgaagggg tcgcctcggg
     4201 tcaacgaaac ctccttaccg aagcggaatc atcgcgcacg aaaaaaacgg aattattaat
     4261 tgcccgggaa tcgcttgaaa ccgagaaaaa gaacatcaag gagcgtcttg ccgcttttca
     4321 gcaggagatg gatctgaccc aatccgaact tcaggacatg gttcgggaat ctcaggagat
     4381 ttttggcaaa gaaactcatc tggaagcgga gcttcgacaa aaggaagccg aggaatcccg
     4441 agttcatggc attcagatgg aaaaagaaaa cgaacttcaa tcgcttctct ctgaacggga
     4501 aaatgttcta ttggaagttc agcaagtccg ttcccagacg gagcaatatc agcaggggat
     4561 acatcattta caggattcaa tgcgtttgat cgcccagcat attgcgaaag acaacgaccg
     4621 gcttgtgttt ttaggtgaag ggcttgctcg atttgacgcg aaagatgtag agctcgcgga
     4681 agatgagtcg cgatgtcatg gtatggccca ggaatatcaa tctcagcttc gcgaggttga
     4741 tttgagcttg gagcttcttc ggaaggaaaa aacggaagct gaaaccaggc ttgagcaaca
     4801 aaatcaggcg attcacgcgt ctcaggaaaa acaaaagcaa attcaggaag cccagcatca
     4861 aatttctctt caaatcatgg atctggatta tcagttcaag aatgtcgcgg aacgattact
     4921 gcagcgctac cggctcaact tggcggattt ggatccgcag gcgtatccgt ttactccaga
     4981 ggatcttcag aacgttgagg aagatattgc gcagctccag accaaggtcg actctctcgg
     5041 gaccgttaat ttgcttgcta ttgaagaata tcaggaacta aaacagcgtt atgatttcct
     5101 cgtagctcaa aaaaaggatt tggaggatgc ccgggattcc ctgatggaag cgatccggaa
     5161 aattaaccgg acgactaaac agcttttcga agataccttc gcgcaggtcc aggtgacatt
     5221 tcgcgaatat tatcagacgc ttttccgcgg aggagatgcg cgtcttacac tgattgatga
     5281 ggaacatccg ctggattccg ggattgatat tgtggttcgt cctcccggca aaaaacttca
     5341 gaatatgagt ttgctttcgg gaggagaaaa agcgctgact gccgtcgcgc ttctttttgc
     5401 gctattcaaa atacgtcctt cgcctttttg ccttcttgat gaagtggatg ctccgcttga
     5461 tgaggctaat attgaccgtt ttctcgcggt gttgcggtcg tttgttgaca ccacgcagtt
     5521 tctgatcatt acgcataatc gcaaaaccat tgccatgggg gattcgcttt atggcgtgac
     5581 aatggaagag ccgggtgtct ccaagatcgt atccgtgaaa gttcgatcag gtgatgaatc
     5641 tgtgccggga gaggatttgc acccgagcgt cgccacggaa gaggccatgc gcgcgattca
     5701 acatccccag gaaactacgg gcgcggttac ttcttgagta ttccgaatta cctgacactt
     5761 ctgagaatta ttttaactcc ggtttttatt acctctctga tttcttattc tccggaaaag
     5821 gaatatctgc gttattgggc gctcgcgatt tttgcgctgg cggggttgac cgacgcgctg
     5881 gacgggattt tggcgagggt gcttcggcaa aaaacagcgc tcggagaaat tctggatccg
     5941 ctcgccgata aattattact tttaagcgga tatatcagtc ttctttttgt tgactctgtg
     6001 ctctatcatc cgccgctttg gatcacgata gtcattattt ttcgggacgt ggttctctca
     6061 atcggatttt tcaccctcca tttccttcga gtcccatttg acgccaaacc taatctgatt
     6121 ggaaaagcca ccacggtttt ccagatgtcg cttctccttt tgatccttct taagtggcct
     6181 gtggcggtac ctgttgctta cgtgaccggt tttttgacaa ttctttccgg cgttatttat
     6241 gtctcgaaag ctttaaaaag gataccgtca tgatcgttct tttttttctg gccgcttatc
     6301 tcctgggttc gattccattt ggcctcatgg ccggctattt tctaaaggga atcgatattc
     6361 gcgaacatgg gagcaaaaac attggtgcca ccaatgtttt tcgtacagtc ggaaaaaaat
     6421 gggggataac ggttcttttt ttggacgctc tcaagggatg gatcgcatgc cttctgccgc
     6481 attgtttcgg cattgccgac ttgccaactc agattacgtt gctactcgga gtcgcggcaa
     6541 tccttggaca cacatttccg gtatggctga aattcaaggg cggaaaaggg gtagccacct
     6601 ctcttggagt ttttctcgca atttgctggg aagcgacagt ggcaaccttc gggatatgga
     6661 ttctcgcgct gttgataacg cgcattattt cgatggcttc catcgtcgcg gtgttaactt
     6721 ttccggtcat ggtcgcgttt ttctattggg gcaatcccga tgtttactgg cttcttccca
     6781 tcagcatttt tctgggagct tttatcctat acacccaccg aagcaatatt tcccgccttc
     6841 gccaagggat tgagaaaaag atcttctaga atattgactt ttcttataaa tcatatagat
     6901 tattcattat atgaaatttg cgtaaggcgc ctttcgatag ggaataatct ttacagaaag
     6961 taaaccggcc gatagaatca gcgcttctgt tgtcaggtaa aatagaacta aaaaaatgtt
     7021 atctttgctt atcgatgtat aggcttcttt cgaagctttg ccgcgtgctc tgtggtggca
     7081 gaagaactga taaaggagaa tgacgtatgg caaccgaacg aaagaaaaca gaaaaacccg
     7141 cgtccgatcc caaaaaaaca acgggagata aggaaaaggc gcttgaactt gcgctgtccc
     7201 aaattgagaa acaatttgga aaaggcgcca ttatgaagct gggcgattct cgtcacgaaa
     7261 atgttgtgcc aattccaacc ggagcgctca gtctcgatat agcgacagga atcggaggaa
     7321 tccctcgggg aagaattgtg gaaatttacg gtcctgaatc cagcgggaag accacgctga
     7381 ccctcaacgt aatctcgcgt attcaatctc aggggggagt tgcggcgttt attgatgccg
     7441 aacatgccct ggatcccgtc tacgcaaaaa aaattggcgt aaaggtcgat gatcttctga
     7501 tttcacagcc tgattcggga gagcaggggc ttcagatcgc tgaaatgctt gtgcgatcca
     7561 acgcggtaga tctcatcgtc gtcgactcgg tcgc
//
LOCUS       DAOSIO010000064         7541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_8968_length_7541_cov_4.274112, whole genome shotgun sequence.
ACCESSION   DAOSIO010000064 DAOSIO010000000
VERSION     DAOSIO010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7541
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8968_length_7541_cov_4.274112"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="PK590_03605"
     CDS             complement(<1..1006)
                     /locus_tag="PK590_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG23713.1"
                     /translation="METRSEETVKTLTNDKEYMNRYFTIGELYYAKRLVIRGLRDGGD
                     VALAGKIPELNWDIKEKEGAFFEDVAREVSAGRKFAFEILAEQFKLESYEKRVLLFFL
                     FLEFFHVADNLCAEMELLEVMDLEDSPISRMKDFKYIGKSACLFRSRLLVGESIKTET
                     SATFSVALSGRFLEKFAGLMNGERGLPQNDKPEESLDSSRDCNEIGMLKVPEYKLEDV
                     VLTDGIKDKVMFFLLSMKEPSWENLGIDTTIKKGKGLNFLFYGPPGTGKSMLAEGVAS
                     YLGKKLLLVETPKIFSRWVGETDKAIANIFTTAKKHDLVICMDEADSLLYNRQYAGQE
                     H"
     gene            complement(1166..1426)
                     /locus_tag="PK590_03610"
     CDS             complement(1166..1426)
                     /locus_tag="PK590_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23714.1"
                     /translation="MKKDKAKALNELRSILQTFAAVREEGIFKLDAVDEVVRRTGLPM
                     DIVIGLLHASGVYKLKEGSTIQDDMLFDTRFKVDEMQPWKPV"
     gene            1586..1849
                     /locus_tag="PK590_03615"
     CDS             1586..1849
                     /locus_tag="PK590_03615"
                     /inference="COORDINATES: protein motif:HMM:NF016109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQ-loop repeat-containing protein"
                     /protein_id="HOG23715.1"
                     /translation="MITEVVGWLGVGFGVSVSIPQLIKSVRARSTNGLSKHTYQLLLA
                     TIICYLVRAIAIGEIVFIVSNVCGLIVTTAVLYLFRKYPAHKA"
     gene            complement(1862..2410)
                     /locus_tag="PK590_03620"
     CDS             complement(1862..2410)
                     /locus_tag="PK590_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23716.1"
                     /translation="MNIRNDMGRFFCIASLMCLVGFIGGEGFCGETVATSKRELLLYR
                     VDDDYERHPDGRVPVDESLPIQQKMRLLLDEISRRHYPDLPPIEILETLKTKDGLVAV
                     LNLTEQEQEFFKSRWYQKFGGSLGWKQTYCQLIETPLQPDYPGFWFSGVKLYWNGKPL
                     DEDIEISGVIFRVKSKGNSLEQ"
     gene            complement(2434..3495)
                     /locus_tag="PK590_03625"
     CDS             complement(2434..3495)
                     /locus_tag="PK590_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOG23717.1"
                     /translation="MKGTESQTWLGGRLALKGEGNYAGSTEAVGYHHNGCLRFRYPMN
                     NGKFHGVCRCWAGNEQLIVEEFYEHGKLHGVSKEWYDSGAIKKQSMHLKGEPCSEREW
                     HENGQAASESVFVKGKLSGTRRAWYRDGQPQSEYGYRDGLPVWGKQWYLGGQIACEEN
                     FREGKLHGPQKGWYENGIMKHDCRYVDGRPHGVQRTWFPGGRLQSEYPYREGRRHGLC
                     QTWDKTGGTRTRQYCLRDVVIPRNLEVLILAGKLDAKVIIKIKNAAIRRICLEELGYA
                     RFLSQVEHKIVDRQGEQELVKIRWHPREETLCLVKVKCPSTGAFYTLRVPPGMKTVKE
                     AVAWTFGVKSDEYDPIQET"
     gene            complement(3575..4126)
                     /locus_tag="PK590_03630"
     CDS             complement(3575..4126)
                     /locus_tag="PK590_03630"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOG23718.1"
                     /translation="MKISCNKRKAYLMFGMLIGIPKRKRGLVSWLYDAQRVVFIAGAE
                     AIKAGDRRIAARHLWSGMLQVVENNQPSVQYSLVGAIFKKLGVSLGGEVQENRNQMLH
                     DKATMMKAKVCLGNEAKKVIQIAYKESFNLGHSYIGAGHLLLGLVGLNSKAQRDAIEY
                     QDFDLQKVRDAFMDCLRSDISSL"
     gene            complement(4153..4446)
                     /locus_tag="PK590_03635"
     CDS             complement(4153..4446)
                     /locus_tag="PK590_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009149399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23719.1"
                     /translation="MKVRQGDLLIVRVEELPENSVLQNHRVLAEGEATGHMHELDSGE
                     LYEKSGTLYFRVPEDQKTTLTHQEHGPITFEPGVYKVIRQREYEPRGWRRVAD"
     gene            complement(4490..4975)
                     /locus_tag="PK590_03640"
     CDS             complement(4490..4975)
                     /locus_tag="PK590_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23720.1"
                     /translation="MSKIDLMVLRRKKILFETTGKPVITSAAERTHRVCGVCLGVYWY
                     HVPSGKILWSGARGAFHLDPDFLRLDAELFRLINDPLPQEASRKVMDAHPKEHAYRAG
                     WISGRVGSYDGKSFAFIYRNRIYSDKLTGADGVALINKIGEVAPVEVEFLLDHEGFSL
                     V"
     gene            complement(4981..5430)
                     /locus_tag="PK590_03645"
     CDS             complement(4981..5430)
                     /locus_tag="PK590_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23721.1"
                     /translation="MRKELDHKQGGKEARCDDDPLFLKLAKVRNIISFRGRKLVPDAR
                     DIAHYGDVLKGIWWYELPSGDMRYSSKSLSHLDDEFSEVVAPFSGQWVRGRVGDDRGK
                     VYAFLYKCDFKARAIPGAVAGDMLRKLRVKSGLPIRYFVDEKGYSLV"
     gene            complement(5415..6347)
                     /locus_tag="PK590_03650"
     CDS             complement(5415..6347)
                     /locus_tag="PK590_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23722.1"
                     /translation="MKNITRNKYQELRKIVRRNIWANSLEVATRKYQYIYGFTVMGGI
                     VLGKQMKRRKQSLLAPLRDGLLEKYGPPDYDLNHRRTEDWRPVFDLWLKVFDFSGTFE
                     IRITADHNNSQWNAFFKKVRNGDVVFAKGQILFDEKTGRLRLEASWIGLEHEFINYLQ
                     TRPEEFDVAKQITEAVKKGGYYLKWDLYAGFTHPDRPQIREWTVKDLAGIDPMAAVRN
                     FYRGGCRDRDIPGFLDALSFLYRRSRVNWVPRLIVKYFKIQVLKGLGREDVRRYGWTN
                     DKERFFSCFPPLRRKICWPGEKIDNLRLRECVKN"
     gene            complement(6580..7299)
                     /locus_tag="PK590_03655"
     CDS             complement(6580..7299)
                     /locus_tag="PK590_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23723.1"
                     /translation="MTKQQEVILRLEKGPLFIDGGNPEAPLWICAIEYGMEKKGGIKK
                     LKKDFDNTGLKRKPIPNWDKESPQFFEAIAKQPHHRDVAKMLMGYLDKRAEDWSSYIK
                     AGGYGRSKGDFFKLNLFPVRANGQKQWLRKHAELTGFKNRATYSYWARRNYFPRLREL
                     VKEYKPKIVLGTGIGCCVDFLDVFGADDCRCDFVKQGPLMVWKFNKLKRAVVVSRFLS
                     RWSTKDLVKLGKKLKNLLKPQ"
     gene            complement(7363..>7541)
                     /locus_tag="PK590_03660"
     CDS             complement(7363..>7541)
                     /locus_tag="PK590_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015951158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOG23724.1"
                     /translation="HLYLNHLPQAELLLSREPRPLPRVVLSPEVADIFSFGLDDITLE
                     GYNPHDSIPAPVAV"
BASE COUNT         1874 a         2118 c         1458 g         2091 t
ORIGIN      
        1 cgtgctcctg ccccgcatac tggcggttat aaagcagcga atctgcttca tccatacaaa
       61 ttacgagatc atgttttttt gcggtcgtaa aaatattcgc gatcgccttg tctgtttcgc
      121 cgacccatct actgaatatc ttcggcgtct ccaccagcaa aagcttcttt ccgagatagc
      181 ttgccacccc ttccgccagc atgctttttc ccgtgcccgg cggcccgtaa aaaaggaaat
      241 tcaggccctt tcctttcttg atcgttgtgt cgatccctaa gttttcccat gatggctctt
      301 tcatagataa aagaaagaac atgaccttat cttttatccc atctgtaaga acaacatcct
      361 ccagcttata ttctggcacc ttgagcattc ctatttcatt acagtcgcga ctggaatcca
      421 aggactcttc cggcttgtca ttttggggca acccgcgctc cccgttcata agaccagcaa
      481 acttctcaag gaaccggccg ctcaatgcca cagaaaatgt ggcgctcgtc tccgttttaa
      541 tgctttcgcc gacaagcaac cgggatctaa aaagacaggc gctttttccg atatatttaa
      601 aatctttcat ccggctaatc ggcgaatcct cgagatccat aacctcaaga agctccattt
      661 cagcacatag gttatccgcg acatggaaaa actcgagaaa caggaagaaa agaagcaccc
      721 ttttttcata ggactcaagc ttgaattgtt ccgccaggat ctcgaatgcg aattttcttc
      781 cggcagaaac ttcccgcgct acatcctcaa aaaaagcgcc ttctttttcc ttaatgtccc
      841 agttcagttc cgggatcttc ccagcaagag cgacatcccc accgtctcta agcccgcgga
      901 ttaccaaacg cttcgcgtaa tacaactcac cgatcgtaaa ataccgattc atgtactctt
      961 tgtcatttgt aagagttttc accgtttctt cacttcgcgt ttccatattt tttaccagtt
     1021 ctcctgtgtc gttttctcga aagcaatacg ccttttttat aaattacttc tccaagcggc
     1081 cggccgtcga tgtcataagc aatccattta ccattaagat catgaaacga gtgctccttc
     1141 aaaatcctat cgggttttgc ggatttcata ccggcttcca aggttgcatc tcatcaacct
     1201 tgaaccttgt atcaaaaagc atgtcatctt ggattgttga gccttccttt agcttataga
     1261 cgccagatgc gtgcaacaaa ccgatcacaa tatccatcgg caaccccgtt cgtcgaacca
     1321 cctcatctac cgcatccagc ttaaaaattc cttcttctcg cacggccgcg aatgtttgaa
     1381 gaatggaacg cagttcgttt aatgcctttg ccttatcttt tttcatatgc acacctcttt
     1441 caagttagct tcatgataac cgcatactgc gacaaggtca tgtcgcgcat atcttgttgc
     1501 ccgtttttta ttccctacta tccgtagtcg acacagggct gtcgcgctta tccgttatta
     1561 tgttcataga aaagagaggt gttttatgat cactgaagtt gttggatggc tgggggtcgg
     1621 gttcggagtt tcggtcagca ttcctcaact gatcaaatct gtaagggccc gctcgacaaa
     1681 tggtcttagc aagcacacct atcaactgct tcttgcaaca attatctgct atcttgttcg
     1741 ggccatcgcc ataggcgaaa ttgtattcat cgtgagcaat gtctgcggat tgatcgttac
     1801 gacagctgtc ctctacctgt tcagaaaata cccggcgcac aaagcatgac tgtttggcgc
     1861 tttattgttc gagtgaattt ccttttgact tcacgcgaaa aataacgccg gaaatttcaa
     1921 tatcttcatc gagtggcttg ccattccaat acagcttaac tccggaaaac cagaagccgg
     1981 gataatccgg ctgaagaggc gtctcgataa gctgacaata ggtttgcttc catcccagcg
     2041 atcccccgaa tttctgatac catcttgatt tgaaaaattc ctgctcttgc tccgttaagt
     2101 taagaacggc caccaatccg tcctttgtct tcagcgtctc gaggatttcg atcggaggaa
     2161 gatccggata atggcgacgg gatatctcgt ccagaagaag tctcattttc tgctggatcg
     2221 gcagtgattc atccaccggc actctcccat ccggatgtcg ctcataatca tcatccacgc
     2281 ggtacaaaag aagctctcgc ttacttgttg cgaccgtctc accgcaaaat ccttcaccgc
     2341 caataaaacc caccaaacac ataagactag cgatacaaaa aaatcttccc atgtcgtttc
     2401 taatgttcat atttcctccc gcctcctccc gagttatgtt tcctgaattg ggtcatattc
     2461 atctgattta acgccaaaag tccaggccac agcttcttta acggttttca tccccggagg
     2521 aaccctgagc gtataaaaag cgccggtcga gggacatttg actttcacaa gacaaagcgt
     2581 ctcctcccgg ggatgccacc ggatcttcac gagctcttgc tccccctgcc ggtcaacaat
     2641 tttatgctcg acctgagata gaaatctggc atagcccagc tcttcaaggc aaattctcct
     2701 gatcgccgcg tttttgatct tgataatgac ttttgcgtca agcttccccg caagaatcaa
     2761 gacttcaaga tttctgggga tcaccacatc ccggagacag tattgccggg tgcgtgtccc
     2821 tccggtttta tcccatgtct ggcagagccc gtgccgcctt ccttcccggt atggatattc
     2881 ggattgaagc cttccgcccg ggaaccaggt tctctggact ccgtgaggcc tgccgtcgac
     2941 ataccggcag tcatgcttca tgatcccatt ctcgtaccaa cccttctggg ggccgtgcaa
     3001 tttcccctcc cggaaatttt cctcgcaagc gatttgtcct cctaaatacc actgcttgcc
     3061 ccaaacaggc aaaccgtcac gatatccgta ttcgctttgg ggctgtccgt ctcgatacca
     3121 cgcccggcgc gttcctgata gctttccctt cacaaacacg ctctccgaag cggcctgacc
     3181 gttctcgtgc cattcccttt cagaacaggg ctctcccttc aaatgcatcg actgtttttt
     3241 tatcgcgccc gagtcatacc attccttgct gacaccgtga agcttcccgt gctcgtagaa
     3301 ttcttcaacg ataagctgct cattcccggc ccagcaacgg caaacaccgt ggaattttcc
     3361 gttattcatc ggataccgga atcgaagaca accgttgtga tgatacccaa ccgcctccgt
     3421 gctcccagca taattcccct cacctttgag ggcaagccgc ccgccgagcc aggtttgaga
     3481 ttccgtacct ttcataatcc gcgatacgac cctatctccg aaactcatat tcccactgac
     3541 gaattgattc accgcaatta ttacttctct gtttttacaa actagaaata tcgctacgga
     3601 gacaatccat aaacgcatct cgaacttttt gaaggtcaaa atcttgatac tcgatagcat
     3661 ctcgttgagc tttcgaattt agcccaacaa gtcccaacaa aaggtgtccc gccccaatat
     3721 agctatggcc aagattgaac gattctttat atgcaatttg aattactttt ttcgcttcat
     3781 tccctagaca aacttttgct ttcatcatcg ttgctttatc atgcaacatt tgattccgat
     3841 tttcttgaac ttctcccccg agagaaaccc cgagcttttt aaagatagcg ccaactaagc
     3901 tgtattgcac acttggctga ttattttcta caacttgtag cattccggac caaagatgac
     3961 gggcggcaat ccgcctatcc ccggctttta tagcttcggc accagcgata aaaacgactc
     4021 tttgggcatc gtacagccat gaaactaagc ctctttttct ttttggtatc ccgatcaaca
     4081 ttccaaacat taaatatgcc tttcttttgt tgcaactgat tttcaaggat tcgattttct
     4141 ctcttatttt tcttagtcag caacacgccg ccacccccga ggctcatact cacgctgccg
     4201 aattaccttg tacacgccag gctcgaatgt aattgggccg tgttcttgat gcgtcagcgt
     4261 ggttttctga tcttcgggaa cccggaagta aagcgtcccg cttttttcat aaagctcccc
     4321 cgaatccagc tcgtgcatgt gcccggtcgc ttcaccttcc gcaagcaccc tgtggttttg
     4381 aagcacactg ttctccggaa gctcttctac tctcacgatc aaaagatctc cttgtcttac
     4441 tttcatttcc gaatcctcct atttgttttt tatatctaat gcaactcgcc tagaccaaac
     4501 tgaacccctc atgatcaaga agaaattcaa cttcgacagg agcaacttca cctattttat
     4561 tgatcagcgc cacaccatcg gccccggtca atttgtcact atagatcctg ttcctgtaaa
     4621 taaaagcaaa gctcttccca tcgtagctcc cgaccctgcc gctgatccaa cccgccctgt
     4681 atgcatgctc cttcggatgg gcatccatca ccttacgact tgcctcttgg ggcaaaggat
     4741 cgttaatgag cctgaaaagc tccgcatcaa gtctcagaaa atccggatca agatgaaacg
     4801 caccgcgggc acccgaccat aagatcttcc ccgaaggaac atgataccaa tacactccga
     4861 gacacacccc gcaaactcgg tgcgtcctct cggcagcgct tgtaatgacc ggcttcccgg
     4921 ttgtttcgaa aaggattttc ttccttcgca ataccatgag atcaattttc gacataagcg
     4981 ctaaaccagg ctgtatcctt tctcgtcaac aaaataccgt atcgggagtc ccgactttac
     5041 ccgaagcttc cgcaacatat cgcccgcaac ggcccccggg atcgctcttg ccttgaaatc
     5101 gcacttgtaa agaaatgcgt aaaccttgcc ccgatcgtct cccacacgcc ctctcaccca
     5161 ctgccccgaa aaaggggcca cgacctccga aaactcatca tccagatgag acagggattt
     5221 tgaggaatac ctcatgtcac ccgacggcag ttcataccac cagatgcctt ttaggacatc
     5281 gccgtaatgc gcgatatctc tggcgtcagg aacgagtttt cgcccgcgaa atgaaatgat
     5341 gttccggact ttcgcaagct tcaaaaagag cggatcgtca tcacagcgcg cttctttccc
     5401 cccttgcttg tggtctagtt ctttacgcat tctcttaacc tcaaattatc aatcttttct
     5461 ccgggccagc aaatttttct tcgaaggggc ggaaaacacg aaaaaaatct ctccttgtca
     5521 ttagtccatc cataccgcct gacatcttcc cgccccaatc ctttcaggac ctggatcttg
     5581 aaatatttca cgatcagtct cggaacccag ttcacccggg atctccggta aagaaaagaa
     5641 agtgcgtcaa gaaatccggg aatatccctg tcccggcatc ctccccggta aaaattcctg
     5701 accgcagcca tcgggtcgat cccagcaagg tccttcaccg tccattcacg gatctgcggc
     5761 cgatcgggat gagtaaaacc cgcatatagg tcccatttca gatagtagcc gccctttttg
     5821 accgcttcag tgatttgttt tgcgacatcg aattcttccg gccgcgtttg caggtaattg
     5881 ataaattcgt gctccaaccc gatccaggac gcttcaagcc ggagtcttcc ggtcttttca
     5941 tcgaacaaaa tctgcccttt cgcaaaaacc acatccccat ttcttacttt tttaaaaaat
     6001 gcgttccatt gagaattgtt atgatctgcg gttattctaa tctcaaatgt ccccgaaaaa
     6061 tcgaacactt ttagccacag atcaaaaacc ggccgccaat cttcggttcg acggtgattc
     6121 agatcgtaat caggaggtcc gtatttctcc agcagcccgt cccgaagagg tgctagaaga
     6181 gactgcttcc gccgcttcat ttgtttcccg aggacaatcc cgcccataac ggtaaagccg
     6241 taaatgtatt ggtacttcct ggtagctact tcgagggagt tcgcccagat gttccggcga
     6301 acgatcttgc gaagttcttg atatttattg cgggtaatgt ttttcatggg cgatattata
     6361 acccgcccac gcgacagcgt atcgtcgcct caaaaaattt ataaaaacga taccccgtcc
     6421 taatttcttt gagaaatgga ttaagatcag gacagcatcc catcgttata attcaattta
     6481 caaaaaacag gatgctgtct taacttcact atagaggatt atctaaacgg aagaaggcag
     6541 aatttcgcgg ctactaaaaa atatataagg aatgcgtact tattgaggtt taaggaggtt
     6601 cttcaatttc ttacccaact taacgagatc ctttgtgctc catctgctta aaaaccgaga
     6661 tacaactacc gccctcttca acttattaaa tttccaaacc atcaaaggcc cttgtttaac
     6721 aaaatcacac cggcaatcat ctgctccaaa aacatccaaa aaatccacac aacatccgat
     6781 ccccgtccct aatacgattt tgggtttgta ttctttgaca agttcccgga gccgtggaaa
     6841 atagttgcgt ctggcccaat aactgtaagt tgcccggttt ttgaatccgg taagctctgc
     6901 gtgcttacgc aaccactgtt tctgcccatt ggcgcgtacg ggaaaaagat tcagcttgaa
     6961 aaaatctcct ttggaccggc cataacctcc tgcttttatg taggaactcc aatcctccgc
     7021 cctcttgtca agatacccca taagcatttt tgccacatct cgatgatgcg gctgtttagc
     7081 tatagcttcg aaaaattgag ggctctcttt atcccagttt ggaatgggct tccgtttaag
     7141 tccagtatta tcaaaatctt tctttagttt tttgatcccg cctttctttt ccatcccata
     7201 ttcaattgcg caaatccaaa gtggcgcttc ggggttccct ccatctataa acagaggtcc
     7261 tttttcaagt ctcaaaatta cttcttgttg tttcgtcata gaatcctttt catgcaccat
     7321 ggaatttgat cccaagccac tcccctcatt ccaaaacacg ctttaaaccg caactggtgc
     7381 ggggatcgaa tcgtggggat tgtatccttc cagtgtaata tcatcaaggc cgaatgagaa
     7441 aatatcggcg acctccggcg agaggactac tctcggcaaa gggcggggct ctcgcgacag
     7501 cagaagttcc gcctgaggca aatgattcag gtaaaggtgc g
//
LOCUS       DAOSIO010000065         7430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_9136_length_7430_cov_4.136678, whole genome shotgun sequence.
ACCESSION   DAOSIO010000065 DAOSIO010000000
VERSION     DAOSIO010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 7430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7430
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9136_length_7430_cov_4.136678"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1455)
                     /locus_tag="PK590_03665"
     CDS             complement(<1..1455)
                     /locus_tag="PK590_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG23725.1"
                     /translation="MGLPSLSLKRPVLTAIVFGAVLVFGFISFSRLKVELYQGQNQGI
                     VSIVVRARGGLPSQDVEKMITKPIEEAVGTVSKMKKLYSTSREAESRVTMEFEPGTNM
                     KFAAPEIREKFARVRPLLPKEIEKPVIANYDDAQAAILVFAVTSETLTPEEIREMVDI
                     KLKPVLSRVDGVASVEVYGGRERKILVELDRDKMVAYNISIERVMDVLGQSNISLLAG
                     NVETGQFEYAIRSMGTFLSVEEIGDIGIKATRQGSIISLKEIATVKDSYLEATDQARL
                     NLEQNVTVYVKKTSLANTLNVADDLARVLDLFEIENEDSVKIVIVNDRADTIRRAIND
                     VKLALLMGLVLTMLVIYFYLGRWRLAGIILAVIPCSLMITFICMDAAEISINVMTLSG
                     LSLAVGMLVDTAIVIMENIFSKKEKGMTTAQSILQGSEEVWLPLLASMATTLVVFVPV
                     VLIDKTIQLTYGGFAFTICAALVASFFSALMLIPI"
     gene            complement(1459..4725)
                     /locus_tag="PK590_03670"
     CDS             complement(1459..4725)
                     /locus_tag="PK590_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG23726.1"
                     /translation="MSLPEFSIKKKVTVYMVTAAFILLGVLAFQRLPQELFPPITFPQ
                     VTIVTDYSNAAPEEIETLITRVIEESIGSVAGLKRIESSLREGRSTIIASFNWGQDID
                     FAALAIREKVDLIKERLPKESDDPVVLKFDPLSRPILILSVTGTNIPPVRLKFLAEKI
                     LKDRLEKVEGVASVTISGGVNREILVEIDMPRLQANHLSLLEVVDSLDEANISYPAGS
                     IKKGLYEYLIRTVGDFRSVAEIHHAVVGVDNVEKARREEASFIERDYSEGPRRTIDRS
                     REEVEQKMLEKRLVLVKDIGNAIDGLAEKTSISRYNDSENISISIQKQSNANTIQVVD
                     RLRRELGALREDIESRGVQSKIIYDHSVFIRQTLENLVHEAVMGGLLALATLWIFLRA
                     LSVSFLVIISIPLSMLGVFFMMAMGNITVNIMSLGGLAISIGMVIDTSIVVLENIFRR
                     RQLGDLPEKGAVEATNEVTWPVVSSNLSTIAVFFPLIVFVPGVAGQIFKDLSWAIIFS
                     QIFSTFIPLTLVAMLSTYINVPQKDYRPFSLTGSLDRYILGLPTEKEQNRLMGTILLS
                     VIVLLLLSIFLVVPTIDREVLPKLDQGQFSVKVDMPLGTRLDVTDQVCRTLEKKIRTI
                     PEVEDISTSIGSEKTGRGEIKIETLRPSQALIFVSLKKDRKRSSSQVVSELSDMTKAL
                     DLAAQGAKIEFMLQESEFAVVGTGSKPILVEVKGYDFSTMEKLVHRLKGKLSTIPGVM
                     NVQDDIGKKSPETKLEINKKRAALYGISALDISLTAKAAIEGVVATQYREGGREVDVR
                     VRLNEADRSDIDNLGDLLLYSKILDVHVPLKEIALIERGEGPSEIKRSDQERTVTISA
                     EISKNRKDKDVLAAVQKLLASLRVPDDIPQDFQVKLSGKAKEIKENFGGVIFAFVLAV
                     MLVYMIMAAQFESFLQPLIIMITIPLSFAGAVLAIKISGHSFNVISLLGLVLLEGVVV
                     NNGIVLMEYINQLRAEGVEMVEAVWQATRTRTRPVLMTSTTTIMGMLPVALGIGEGGG
                     ILAPLSVTIMGGLFSSTVLTLLVLPCLYILVNRILDRFFSYEDLPEADETIEGSLPQT
                     "
     gene            complement(4728..6233)
                     /locus_tag="PK590_03675"
     CDS             complement(4728..6233)
                     /locus_tag="PK590_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG23727.1"
                     /translation="MESNGKDPQGGRLPDQKNAKSNPDTLKNIAARLKNPGGTTSQNL
                     KEKTPVSVSPIRISSPKEPRKPVEQPWILRNLRKVVILAVVVLVVFFGARAIVGFFQK
                     TSKPSLQETMQQQRQALKDAPVAVKGFKVGRFNYEDALNVLGTIKGAMEVKLSFEVPG
                     VISSINYREGERYEEGALLVSLRQDDILLRLKRAQAAFNKAETQAKVIENEVVENEKL
                     LRMGAIPASTFEKKKLELDAAKFEAETQRLEMKANEAMLEKSNLYAPSPGMLGGLNVQ
                     EGENVSQSTLIGIHINTAFVYAEFGIVERDVQKISVGQRAKVFVDAYPDKNFDGVIEN
                     VAPQISGTSRTSTARIRVENPERLLMPGMFARVRILLYTKKDALVVPTDAVQGGEGEK
                     WVYVVKLDDPGGAAMKAEEALPRGGLTGLAAMAMKKTQGTSEATQPEATSRKKEEGYV
                     EKRPITVGYTRTDYTQIDAGITEGEVVVLTGFERLEDAKKVRLVETQEAEL"
     gene            complement(6223..>7430)
                     /locus_tag="PK590_03680"
     CDS             complement(6223..>7430)
                     /locus_tag="PK590_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG23728.1"
                     /translation="TFLQVKSSLEAAKKQYDKIISDLIFDLSRAYFEYQRTRQTLEEH
                     ESVVAEMKKYADMSEEKFRMNLIAEIEHLNVQSLYSQMQFDLENAKQDFEISKLDIQR
                     FLDLSPDDTINLATTYDLSMILDKTLATEPAGSTALSEWEGVARLLGEELTPELPKII
                     DLSYANRPELKVEAAKLQVTRLGERIRWGEFLPKAFLTWEAGKLGEAYRGIYIYDGYK
                     DDPDLKREWRLMLELNWNVAGNKVSYTYDKDKKAPSISQYLAGAGTQIRKNNLNIGIL
                     DGLDAYVNVKQAEVDKLNQVVELEKAEKQVIQDVKQAFYDYQKSLIQVRSTTKRAKWR
                     ERLRDLAKHRLERKEVEISEYMQAEADLVRERGDLHRTLKDYYSAKSALNHAVGIQDY
                     LKLEGSHGI"
BASE COUNT         1804 a         2063 c         1728 g         1835 t
ORIGIN      
        1 aatcgggata agcatcaagg ccgaaaaaaa tgacgccacc aacgcggcac aaatcgtgaa
       61 cgcaaatccc ccatatgtta actggatcgt cttgtcgatt aaaacaaccg ggacaaatac
      121 caccagggtg gttgccatgg aagccaggag cggaagccaa acctcttcac ttccctgcaa
      181 aatcgattgg gccgtcgtca ttcctttctc ttttttggaa aaaatatttt ccatgatgac
      241 tatcgcggta tccaccagca ttcccaccgc cagcgaaaga ccgctgagcg tcatgacatt
      301 gatgctgatt tcggccgcgt ccatgcaaat aaacgttatc atcagtgaac aaggtatcac
      361 cgctaaaata attcccgcga gacgccatcg cccgaggtag aaatagatga caagcatcgt
      421 cagcacaagc cccatcaaca gggccagttt cacgtcatta atcgcccgac gaatcgtatc
      481 cgccctgtcg ttgacgatca cgattttgac ggaatcctcg ttttcaattt caaaaagatc
      541 cagcaccctc gccaggtcat ccgcgacatt cagcgtgttc gcgagactgg tttttttgac
      601 gtagacagta acattttgct cgagattaag acgggcctgg tccgtggctt cgagataaga
      661 atccttcacg gtcgcgatct cttttagcga gatgatcgat ccctgccggg tcgcttttat
      721 tccgatgtca ccaatctcct ccaccgaaag aaacgttccc atactgcgga tggcgtattc
      781 aaattggccc gtttccacgt ttccggccaa aagactgatg ttgctctggc ccagcacatc
      841 catcactctt tcaatggaga tgttataggc caccatttta tcgcggtcga gttcgacaag
      901 aatctttcgt tcccggccgc catacacctc cacgctcgcg actccgtcca cccggctcaa
      961 aacaggctta agtttaatgt ccaccatttc acggatttct tcgggggtca gagtctccga
     1021 ggtcaccgcg aataccagga tagcggcctg ggcatcgtca taattggcga tgaccggttt
     1081 ttcgatttcc ttgggaagga gcggccggac ccgcgcgaat ttttcccgga tttccggggc
     1141 cgcgaacttc atgttggtcc ccggctcaaa ctccatagtc acccgggact ccgcttcgcg
     1201 ggaggttgag taaagttttt tcatcttgct caccgtcccc accgcttcct caataggctt
     1261 tgtgatcatt ttctcgacgt cctgggaggg aaggccgccg cgcgcgcgca ccacaatgct
     1321 gacgatcccc tggttttgcc cctggtagag ttctaccttg agtcgtgaaa atgagatgaa
     1381 cccgaagaca agtaccgcgc caaaaacaat ggcggttaaa actggccgtt ttagggagag
     1441 actaggtaga cccatggatt aagtctgggg taaagaacct tcgatagttt cgtcagcttc
     1501 agggagatct tcataagaaa aaaagcggtc aagaatcctg ttcaccaaaa tataaaggca
     1561 gggaagcacc agaagggtaa gaaccgtcga agaaaaaagc ccgcccataa tcgtgacgga
     1621 aagaggcgcg agaatccctc ccccctcgcc aatcccgagc gccacgggga gcatacccat
     1681 aatcgtcgtc gtggaagtca tcaagacagg acgcgttctc gtccgggtgg cctgccagac
     1741 cgcttccacc atttccaccc cttcagcgcg aagctgattg atatattcca taagcacgat
     1801 cccgttgtta acgacaaccc cctccaggag aacaagtccc aggagcgaaa tcacgttgaa
     1861 cgagtgtcct gaaattttaa tcgcgagaac cgcgcccgcg aaagatagcg gaatcgtaat
     1921 cataatgata agtggttgta ggaaagactc aaactgagcc gccatgatca tgtaaaccag
     1981 catgaccgcc aggacaaacg cgaaaataac tcccccgaag ttttccttga tctccttcgc
     2041 tttcccggac aatttaacct ggaagtcttg cgggatatca tcaggaaccc ggagacttgc
     2101 gagtaatttc tggacggcgg ccaacacatc cttgtccttc cggtttttac tgatctcggc
     2161 tgaaatggtc acggtgcgct cctgatccga tcgtttaatc tcactgggcc cttcacctcg
     2221 ttcaatcagc gcaatctctt tcaacgggac atggacatca aggattttgg aataaaggag
     2281 aagatcacca agattgtcta tgtcagagcg atcggcctca ttcagacgca ctcgtacgtc
     2341 cacctcacgg cccccttccc gatattgggt cgcgacaacg ccctcaatgg ccgccttcgc
     2401 ggtcaaagag atatccaggg cggaaattcc gtaaagggcc gcgcgttttt tattaatttc
     2461 caatttggtc tccggcgatt ttttaccaat gtcatcctga acattcatca ctcccggaat
     2521 cgtcgagagt tttcccttga ggcgatggac caatttttcc atggtggaaa aatcgtaccc
     2581 tttcacctcg accaagatag gcttgcttcc cgtacccaca accgcaaact cactttcctg
     2641 aagcataaat tcaatcttgg cgccctgcgc cgccagatca agagccttgg tcatatcgga
     2701 aagctccgat accacttgag aagaggagcg cttccgatct ttttttaaac tgacaaaaat
     2761 aagcgcttgt gaaggacgga gtgtctcaat tttgatttcg ccccgtcctg ttttttcact
     2821 gccgatcgag gtcgagatat cctccacctc cggaatggtc cggatctttt tttcaagagt
     2881 tcggcaaact tggtccgtca cgtccagacg cgttccaagc ggcatatcca ctttcaccga
     2941 aaactgcccc tgatccagct tcgggagcac ctcgcgatcg atggtgggaa caaccaaaaa
     3001 aatcgacagc agtagcaaca caatgaccga aaggaggatc gtccccatga gccggttttg
     3061 ttccttttcc gtgggaagac cgagaatata ccgatccagg gaaccggtaa ggctgaacgg
     3121 gcgataatct ttttgaggta cattgatata ggtcgaaagc atcgccacaa gggtaagtgg
     3181 gataaaagtt gaaaaaattt gtgaaaaaat aattgcccac gaaaggtcct tgaagatttg
     3241 tcctgccact ccggggacaa atacaatgag cggaaaaaaa acggcaatgg tcgacaggtt
     3301 ggaagaaaca accggccagg tcacctcgtt ggtggcttcc acggctccct tttcgggcaa
     3361 gtctccaagc tggcggcgcc ggaatatgtt ttcaagaacc acaatgctcg tatcgatcac
     3421 catcccgatc gaaatggcaa gaccgccaag cgacataatg ttgaccgtga tattccccat
     3481 cgccatcata aaaaagacgc cgagcatact gagcgggatg gaaataatca cgaggaaaga
     3541 gaccgataaa gctctcagaa agatccagag cgtggccagg gccagcaatc cgcccatcac
     3601 cgcttcgtga acgagatttt ccagcgtctg acgaataaac acggaatggt cataaatgat
     3661 cttgctctgg acccctcgcg actcgatgtc ctcgcggagc gcgccaagct cccgtctcaa
     3721 acggtccacc acctggatag tgttggcgtt cgattgtttc tggatggaaa tggaaatatt
     3781 ttccgagtcg ttgtagcggg agatactggt tttttcggcc aagccatcga tcgcgtttcc
     3841 gatgtctttg acgagcacca gacgtttttc gagcattttc tgctccactt cctcgcgcga
     3901 acgatcaatc gttcttcggg gaccttcgga gtaatcccgc tcgataaaac tagcctcttc
     3961 acgccgggcc ttttcgacat tgtcaactcc caccaccgcg tgatgaatct cagcgaccga
     4021 gcgaaaatcc ccgaccgtgc gaatcagata ttcataaaga ccctttttaa tacttcccgc
     4081 gggataggaa atattcgctt catcaagaga atccaccacc tcaagcaggg acagatgatt
     4141 ggcctgcaaa cgcggcatgt cgatctcaac cagaatttcg cgattcacgc cgccggaaat
     4201 cgtgacggag gcgacccctt caactttttc aagccgatcc ttcaggattt tttccgcaag
     4261 aaatttcagc cgtaccggag ggatattggt gccggtcacc gataaaatca ggattggccg
     4321 cgaaagagga tcaaatttga ggacaacggg gtcatccgat tccttcggca aacgctcctt
     4381 gatcaaatcg accttttcac ggatggcaag cgccgcaaaa tcaatgtcct gtccccagtt
     4441 gaaagaggcg ataatggtgc tccggccttc gcgcaaactt gattcaatcc gttttagccc
     4501 cgctactgac ccgatggact cctcgatcac gcgcgtaatg agagtctcga tttcttccgg
     4561 cgccgcgttg gaatagtccg tgacaatcgt gacctggggg aacgtaatgg gaggaaaaag
     4621 ttcctgggga agtctttgaa aagcgaggac gccgagcagg atgaacgcgg ccgtcaccat
     4681 gtagaccgta acttttttct tgatcgaaaa ttcgggcaaa ctcatcttca gagttcggct
     4741 tcttgggttt caaccagacg gactttcttc gcatcctcca gtctctcaaa tccggtaaga
     4801 acaacaacct ctccttcggt aatacccgcg tcaatctgcg tataatccgt ccgtgtgtaa
     4861 ccaaccgtaa tcggccgttt ttccacatag ccctcttctt tttttcggga agtggcctca
     4921 ggctgggtcg cttcagaagt tccctgcgtt tttttcatgg ccatcgcggc cagcccggtc
     4981 aacccacccc gcggaagagc ttcttcggct ttcatggccg cgccccccgg atcgtcaagt
     5041 tttaccacat aaacccattt ctctccctca ccgccctgga ccgcgtccgt cgggaccacc
     5101 aaagcatctt ttttggtata aagcaaaatc cggacacggg cgaacatacc cggcatcagc
     5161 aaccgctcag gattttcaac ccggatccgg gccgtcgacg tacggctcgt ccccgaaatc
     5221 tgcggcgcca cattctcgat cacgccgtca aaatttttgt cgggataggc gtccacaaac
     5281 accttcgccc gctgacccac cgaaatcttt tggacgtcac gctccacgat tccgaactcg
     5341 gcgtaaacaa acgccgtatt gatgtgaatc ccgatcagcg tgctctggga gacattttct
     5401 ccctcctgga catttaaccc acccaacatt ccgggggaag gcgcgtaaag gttggatttt
     5461 tcgagcatcg cctcgttcgc cttcatttca agacgctgag tttcagcttc aaatttcgcg
     5521 gcatccagtt caagtttctt tttttcaaag gtagaagccg gtatcgcgcc catcctgaga
     5581 agtttttcat tttcaaccac ttcattctcg atgactttgg cctgggtttc agctttatta
     5641 aacgccgcct gggcccgttt caaccggagg agaatatcat cctgcctcaa cgacaccagg
     5701 agcgcccctt cctcgtaccg ctccccttcc cggtagttga tagagctgat cacgccgggc
     5761 acttcaaaac tcaacttgac ctccatggcc cccttgatgg ttccgagcac attgagagcg
     5821 tcttcataat taaaccgtcc tactttaaac cccttgaccg ccacgggagc gtctttcagc
     5881 gcctgcctct gttgttgcat ggtttcctgc aaggaaggct tggatgtctt ctgaaaaaaa
     5941 ccaacaatcg cgcgcgcgcc aaaaaacacc accaatacga caacagccag aatcaccacc
     6001 ttcctgagat tacgaaggat ccacggctgt tcaacaggtt tccggggttc tttcggcgaa
     6061 gaaatccgga ttggcgaaac gctaaccggc gttttttcct tcaaattctg actggtcgtt
     6121 cccccgggat tcttcaggcg ggccgctatg tttttcaacg tatcgggatt tgacttggcg
     6181 tttttctgat ccggcaacct tccaccctgc ggatcttttc cgttagattc catggcttcc
     6241 ttccagtttt aggtagtcct gaattccgac agcatgattg agtgcggatt ttgcggaata
     6301 ataatccttg agagtccggt gaagatctcc ccgctccctg acaagatccg cctccgcttg
     6361 catgtattcg gagatctcaa cttctttccg ctccaggcgg tgcttcgcga gatcccgaag
     6421 cctttcgcgc catttggccc gtttggtcgt ggagcgaacc tggatcaggg atttctggta
     6481 atcataaaaa gcctgcttga cgtcctgaat gacctgcttt tcggcttttt ccagttctac
     6541 cacctggtta agtttgtcca cttcagcctg cttgacgtta acgtaggcgt ccagtccgtc
     6601 aagaatcccg atattgagat tattcttccg gatttgagtc cccgcgcccg ccaaatattg
     6661 tgagatcgac ggcgcctttt tatccttgtc gtaggtataa ctgaccttgt tgcccgcgac
     6721 attccagttc agttccagca tgagccgcca ctcgcgttta agatccggat catctttgta
     6781 accatcatag atgtaaatac ccctgtaagc ctctcccagt ttgccggctt cccaggtcaa
     6841 aaaggctttc ggaagaaatt caccccatcg aatccgctcc ccaagtcgcg tgacctgaag
     6901 tttggccgct tcgactttta attccggccg attagcgtag gaaagatcga tgatcttggg
     6961 aagctcggga gtcaattctt cacccagaag tcgggccacg ccttcccatt ccgacagggc
     7021 tgtcgatcca gcgggctcag ttgccagagt tttgtcaagg atcattgaaa ggtcataggt
     7081 ggtcgccagg ttgatggtgt catccggaga gagatccaga aatctctgga tatcaagttt
     7141 tgaaatttca aaatcctgtt tcgcgttctc cagatcaaat tgcatctggg aataaagcga
     7201 ctggacgttg agatgttcaa tttcggcgat cagattcatc cggaattttt cttcggacat
     7261 atccgcgtat tttttcattt ccgcgacaac cgactcatgc tcctccagcg tttgtcgggt
     7321 tcgctgatac tcgaaataag cgcgggaaag atcaaaaata aggtccgata tgattttgtc
     7381 atattgtttt ttggcggcct caagactgga ttttacctgc aaaaaagtat
//
LOCUS       DAOSIO010000066         7395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_9197_length_7395_cov_3.976158, whole genome shotgun sequence.
ACCESSION   DAOSIO010000066 DAOSIO010000000
VERSION     DAOSIO010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9197_length_7395_cov_3.976158"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..595
                     /gene="tnpA"
                     /locus_tag="PK590_03685"
     CDS             164..595
                     /gene="tnpA"
                     /locus_tag="PK590_03685"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOG23729.1"
                     /translation="MKLQRQAHVVYKTQYHVLWVTRYRRKILVKGIAEYFKKAIREVR
                     EFYPDWFIEEIGTDVDHVHLHMVIPPKYAVSKVVGTMKSVTSARLREKFPHFLKKIYW
                     DNGGIWARGFFVSTVGINEAVIRKYVRYQGQQDAGQTQFEW"
     gene            721..2205
                     /locus_tag="PK590_03690"
     CDS             721..2205
                     /locus_tag="PK590_03690"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HOG23730.1"
                     /translation="MTKPPVFSKTRYLEGLQCPKLLWMRCNAKDEFPPIDEATLAVFD
                     QGHQVTELARKLFPGGIEIEGVEDYDALIRRTQDLLPDRVPLFEAAIRVKNLFARPDI
                     LFPNQDGSWDIFEVKSSGKVKDVNLNDTAFQTVCYEKSGLKIRRVHLVLIDTEYVRRG
                     KIDPKSLFKIEDVTKLVRSLTPQVEPNIERMIKIRDHKTCPEVKIGPQCSDPYRCALW
                     DLCWKRIPEDSPLIYNRIRKDKALSFINDGFLKAAEVPEDRLTAATHKIVRVCHATGK
                     SHADVKAIKGFLKGLEFPLHFIDFETVSAAIPLYDNSRPFQQVPFQFSVHVWESWDKE
                     PKHYSFLAEDRSDPRPAFMKSLKSLIGLTGSILAFNMDFEMGCLKRSADALPEYADWV
                     REIEKRFIDLMVPFRRFDYYDPKQMGSYSIKAVYPALVGGAYEGMAISDGGQASREYG
                     RVTFTEGVPDAEKRRIYDGLLEYCKLDTQAMIDVLKVLKDTVKK"
     gene            2222..3169
                     /locus_tag="PK590_03695"
     CDS             2222..3169
                     /locus_tag="PK590_03695"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOG23731.1"
                     /translation="MAKNKSYDKKIFRLMSILNKLNGGGKVRSSEIAKEFNVSIRSVQ
                     RDIELLNQTQFPVVSLEKGIYSFMEGFSLKKMMLTNEEASLLTFFHEIADSLGKNFSE
                     SFRSLVSKVLAKEEDSPFYVKLPETHAHMKNFPFVKELEKAVSDSQKVMIKYETEKGL
                     EEFKLCPLKLINFDGFWYLLAMPDGMRAFWKFRSEKIKGLEMLDENFDPPRSVKSVLD
                     RSVNIWFNEKPGEKIVLEIDKDAAHYFKKKIYFPDQKIVKEKKDGSLVLETYANFNEV
                     IRTIIHWIPDIRVIKPKDLDSRIRDLLNAYLKGAKPRWG"
     gene            3214..4854
                     /locus_tag="PK590_03700"
     CDS             3214..4854
                     /locus_tag="PK590_03700"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing HNH endonuclease family
                     protein"
                     /protein_id="HOG23732.1"
                     /translation="MNQDAKDKIFIPAAKTIEQIFGDADSFFVVPDYQRPYSWEDEQV
                     EALWDDIYDAMNNGDESYFLGPVILTGPDTEERFQVVDGQQRLTTLTILFCVLRDLFL
                     PEDKTIADAIKSLKNEKFRLRFVSQSSEQNRFEAEILQKVKFPSAEISESSKKKNLFA
                     NTAMIFRDKLKELPGEGKGISGFMDYLLKRVIMITITCTHPSFAVKLFQVLNTRGLDL
                     SPADLIKSFLYQKCEPARRAQFLCTWREIETLAASMGEPLADLLTYYEYYLLAANPRE
                     GLFEELTRLWRDSDSNTVVFDFSKFVKSFSEIFQSSLKIINGFWYLPNQFYWKAILTT
                     ARMNGYEDFQQLALLIRRLFYFYWIAGYTSTKTKQMTFNIIQWIKEKKTVDEMACEIK
                     AKMEKDQVLKRVQENLCGEVYGESWLRALLMLVEYEQTDDSKVEFIELDSKLHVDHIL
                     PSEWHKKEEWCQDWTQGDALPRLNSLGNMTLLSGRKNIVASNDPFSVKKEIYRKSHGG
                     LTAFMISQKVLEKERWTKREVDERQSWLLTEVQRIFKI"
     gene            5009..5857
                     /locus_tag="PK590_03705"
     CDS             5009..5857
                     /locus_tag="PK590_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23733.1"
                     /translation="MFEGEKDINIQMRNGYLNIYYKMNNLGKVFCKKGEVGFNIHNEF
                     LPEGKRRKKDYVELVVQDPFLMLPRRANRYQFAINIFSKNNLKLLKRKVAKQAHEEKT
                     YQSKLIWRNQNTIVDAEVAFNEQLREVVDERGKAKTRRTRIDLLNFDCHRNKLVAIEL
                     KMAKNTELYNGKIRNQLQKYTDFLVSNKTDLQQAYKKAIETKIVLGLLEKTSLLARVA
                     AENIEVEPRPLLVVICHNQKGIDACRQKIEDSVRDLALGVYFFGKGGDLNISKKNHKN
                     KALFPL"
     gene            5854..7176
                     /locus_tag="PK590_03710"
     CDS             5854..7176
                     /locus_tag="PK590_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG23734.1"
                     /translation="MNLIIHRGTHEIGGTCVEIATDGGTRIIIDLGMPLSDPRDKTKK
                     LKNFSLTGKTVPELIGMGILPKVSGLYWGVGDEKPVNAIFLSHPHQDHYGLFPYVRKD
                     IPVYLGEDTDKILRASEVFLGDRFGVHDKKVLLKDRTPPDPVEDIKVTPFLVDHSAYG
                     AMAFLIEADGKRVFYSGDFRGHGRKAKLFEKLIADPPPDIDALLMEGTMMGRQAEDTQ
                     TEDELELVIADEARVHEGMKLFICSGQNIDRLVTFYRATKRSNATFVLDLYAVNILQE
                     INDRGTLPVPSPGFKDVKVLFTKHFMKRLLHYKKEDWFTRWRPHEISPAQLQRAGKRC
                     FVMFRDSSVHELKGIIPKDSVMFYSMWSQYTLEPAFEKTLTFLDENKVAFKEVHTSGH
                     AILSDLKRLAGKMKPGIIIPIHTERPEEYRRHFGGKVQCLLDGEAFNI"
BASE COUNT         2086 a         1630 c         1930 g         1749 t
ORIGIN      
        1 ctgcgccggt tttatagccg cgtagcctga agacagagac tggaatctcg caattagccc
       61 aaaaagcgac attggaacgc ctctaatccc gcgggagttc cccacccgtg agggtggggg
      121 tgagagcaag gagccgccct gctgcccatg atacaattgg ggtatgaagc tacaaaggca
      181 ggcacacgtt gtatacaaga cacagtacca tgtgctgtgg gtcacgagat accgaagaaa
      241 aatcctcgtg aaggggatag cagaatactt caagaaggcc atcagggaag tgcgggaatt
      301 ttatcccgat tggtttattg aagaaatcgg cacagacgtg gatcatgtgc acctgcacat
      361 ggtaattcca ccgaagtatg cggtatccaa ggtagtgggg acgatgaaga gcgtgacgag
      421 tgcgcggctg agggagaaat tcccgcactt cctcaagaag atatactggg ataacggtgg
      481 catatgggca cgaggatttt tcgtatcgac agtaggaata aacgaagccg tgattcgcaa
      541 gtatgtccgg tatcaggggc agcaggacgc gggtcaaacg cagtttgaat ggtaggattc
      601 cccacccgta agggtggggt tcttcaaaat attttattta agcgacacaa tgctgtcgcg
      661 aacgttagtt acaatctcct tcgcatgata tagaattcat ctcaaccgaa aggattgatt
      721 gtgacaaaac ctcctgtttt ttcaaagacc cggtatcttg aaggcctgca atgtcccaaa
      781 cttctctgga tgcgttgtaa cgccaaggat gagtttccgc cgatcgatga ggcaacgctg
      841 gctgttttcg atcaagggca tcaggtgacg gagcttgcgc gtaagctttt ccctggcggt
      901 atcgagatcg aaggcgttga ggattacgac gctctgatcc gccgcaccca ggaccttttg
      961 cccgaccggg tcccgctttt tgaagcggcg atccgcgtga agaacctttt tgcccgacct
     1021 gacatccttt ttccaaatca agacggctca tgggatattt tcgaggtcaa gagctccggc
     1081 aaggttaagg acgtcaattt gaacgatacc gcattccaaa ccgtctgcta cgaaaaatcc
     1141 gggctcaaga ttcgtcgagt tcatcttgtg ctgatcgata ctgaatatgt ccggcgcggg
     1201 aagatcgatc ccaaatcgct ttttaagatc gaggatgtca caaagcttgt gagatcgctt
     1261 acgccacaag tcgagccgaa cattgagcgg atgatcaaga tccgcgatca taaaacctgc
     1321 cctgaagtga aaatcggtcc ccagtgcagc gatccgtacc gatgcgcact ctgggacctt
     1381 tgctggaagc gcattccgga ggacagccct ttgatctata accggatccg aaaagataag
     1441 gcgctcagtt ttatcaacga tggatttttg aaggcggcag aggttccgga agaccgtctc
     1501 acggcggcaa cgcataagat cgtgagagtt tgccatgcca cgggaaaatc ccacgctgac
     1561 gtcaaagcga tcaaaggatt cctgaaaggc ctggagttcc cattgcattt cattgatttt
     1621 gaaaccgtga gtgcggccat cccgctttat gataattcaa ggccgttcca gcaggtgccg
     1681 ttccaattct cggtccatgt ctgggaaagc tgggataaag aaccaaaaca ttattcgttt
     1741 cttgcggagg accgaagcga tccgcgccct gcgttcatga aatctcttaa gtctctgatc
     1801 ggacttacgg gcagcatcct ggccttcaat atggattttg aaatgggatg cctcaagcgc
     1861 tctgcggatg cgctgccgga atacgcggac tgggtgagag agatcgaaaa aagattcatc
     1921 gatctcatgg tgccattccg gaggttcgac tattacgatc cgaaacagat gggtagttat
     1981 tcgatcaaag cggtctaccc ggctctggtc ggtggcgctt atgagggtat ggcgatctca
     2041 gacggagggc aagccagccg cgaatacgga cgcgtgacat ttactgaagg tgttccggat
     2101 gcggagaaac gcaggatcta cgacgggctt ctcgagtatt gcaagctcga tacccaagcc
     2161 atgatcgatg tgcttaaggt tttgaaagac actgtaaaaa aataatcaag gggcggaaga
     2221 aatggctaaa aacaaatcgt acgataaaaa aatattcagg ttgatgtcga tcttgaacaa
     2281 gctgaacggg ggcggaaagg tccgatcaag cgaaatcgcg aaggagttta acgtttcgat
     2341 ccgaagcgtc cagcgggaca tcgaactgct gaaccagacg caatttcccg tggtttcgct
     2401 ggaaaagggg atctattcgt tcatggaggg cttctcactc aaaaaaatga tgctcacgaa
     2461 cgaagaagca tcgcttctca cattctttca tgagattgcg gattcgctgg ggaagaactt
     2521 ttcggagtcc ttccggagcc ttgtgagtaa ggtgctggcc aaggaggaag attcgccgtt
     2581 ctatgtcaag ctcccggaga cgcatgccca catgaagaat tttccctttg taaaggagct
     2641 cgagaaggcg gtttctgatt cgcagaaggt catgatcaag tatgagactg aaaaggggct
     2701 tgaggaattc aaattgtgtc cgctcaagct gatcaacttc gatgggttct ggtatctttt
     2761 ggccatgccg gacgggatgc gcgcgttctg gaagttccgg tccgagaaga tcaagggcct
     2821 tgagatgctc gatgagaatt ttgacccgcc tcgaagcgtg aagtccgtcc tcgatcgcag
     2881 tgttaacatc tggttcaatg agaagccggg tgagaagatc gtgctcgaga tcgataagga
     2941 tgccgcgcat tacttcaaaa agaagatcta ctttcccgat cagaagatcg tgaaggaaaa
     3001 aaaggacggc tctcttgttc tggaaaccta tgccaacttc aacgaggtca tccgtaccat
     3061 cattcactgg atccctgata tccgggtcat caaacccaag gatctcgact caaggatacg
     3121 ggatcttctc aatgcgtatc ttaaaggcgc caagcccagg tggggataaa cgcaggggat
     3181 tacgtccaac aaatcaataa aaggggttct gcaatgaatc aagatgcgaa agataagatt
     3241 tttattcctg ccgcaaagac gatcgaacaa atctttgggg atgccgatag cttttttgtc
     3301 gttcctgact atcaacggcc gtatagctgg gaagatgagc aggttgaggc gctgtgggat
     3361 gatatttatg acgccatgaa taacggcgac gaaagttatt ttcttggccc cgtaatcctg
     3421 acaggtccgg atacggaaga gcgctttcag gttgtggacg gccagcaaag actgacaacg
     3481 ctcacgatac ttttttgcgt tctgagggat ctgtttttgc cggaagataa aactatcgcc
     3541 gatgcgatca aaagccttaa aaatgaaaaa ttccgtctgc ggttcgtttc gcaatcatcg
     3601 gagcagaacc ggttcgaggc cgagatcctt caaaaagtca aatttccttc tgccgaaata
     3661 tcagaaagtt caaaaaagaa aaatctcttt gccaataccg cgatgatctt tcgcgacaag
     3721 cttaaagagc ttcccggtga agggaaaggg atttccggat ttatggatta tcttttgaag
     3781 cgggtcatta tgatcaccat cacctgcacc catccttctt ttgcggtcaa gctcttccag
     3841 gtgctgaata caagaggact cgatctatct ccggcggatc tgatcaaaag tttcctttat
     3901 cagaaatgcg aacctgcccg gagggcgcag tttctttgca cctggcggga gatcgaaacg
     3961 ctggccgcga gcatggggga gccgcttgcg gatttattga cctactacga gtattatctc
     4021 ctggcagcaa acccccgcga gggactattt gaagaattga cgcgtttgtg gcgtgatagc
     4081 gactccaata cggtagtttt tgatttttca aagttcgtta aatctttttc ggagattttt
     4141 cagtcgtcat taaaaatcat caacgggttc tggtatctgc cgaaccagtt ttattggaag
     4201 gccattttga cgaccgcccg aatgaacggc tatgaggatt tccagcagtt ggcactgctt
     4261 ataagacgcc ttttttattt ttattggata gcgggataca cttccaccaa aaccaagcaa
     4321 atgactttta atatcattca atggatcaag gagaaaaaga cggttgacga aatggcgtgt
     4381 gaaattaaag caaaaatgga aaaagaccaa gtgctcaagc gggttcagga aaatctgtgc
     4441 ggggaagtat acggagagtc ctggttgagg gcgcttttaa tgcttgtgga gtatgagcaa
     4501 actgacgatt ccaaggttga atttattgaa ctggacagca agttgcatgt ggaccatatc
     4561 cttccgagcg aatggcacaa aaaagaagaa tggtgccaag actggacgca gggtgatgcg
     4621 ctcccacgct tgaattctct ggggaatatg actctgctat ccggaaggaa aaatattgtg
     4681 gcaagcaacg atcctttttc tgtgaaaaaa gaaatttacc ggaagagcca tggaggactc
     4741 acggccttta tgatctccca gaaagtcctg gaaaaagaaa gatggacgaa gcgagaagtt
     4801 gacgaaaggc aatcgtggct tttaaccgaa gtacaaagaa tatttaaaat atgacggcat
     4861 attaaaacgg ccatctcaaa acttccaagt taaggtgggg aaaggacaga taggaaaaaa
     4921 catgagtaaa aaattcgaca ggatcgcatc gacccatccc ttaattgagc gtttgacccg
     4981 aagcatctgg tggaaacagg cgttgactgt gtttgaggga gaaaaagaca ttaatatcca
     5041 aatgagaaat ggatatttaa atatttacta taaaatgaac aacctcggaa aggttttttg
     5101 caagaaaggg gaagtgggtt ttaatattca caatgaattc cttccggagg gcaaacgacg
     5161 gaagaaagat tatgtcgaac tagttgtcca agatcccttt ctgatgctgc cccgcagagc
     5221 caaccgctat caatttgcta ttaatatttt ttcaaagaac aatttgaagt tgttaaagcg
     5281 aaaggtggct aagcaggcgc atgaagaaaa aacgtatcaa tcaaaactga tttggcggaa
     5341 ccaaaacacc atagtggatg ccgaggtcgc tttcaacgaa cagctcagag aagtggtcga
     5401 tgaaagagga aaagcgaaaa caaggcgaac gaggattgac ctcctcaatt ttgattgcca
     5461 ccggaataag cttgtggcga tagaactcaa aatggcgaaa aatacggagc tgtataatgg
     5521 caaaatacga aatcagcttc agaaatacac ggattttttg gtaagcaata aaacagacct
     5581 gcaacaagcg tataaaaagg ccatagaaac aaagattgtc ctggggcttt tagaaaaaac
     5641 cagccttttg gcaagagttg ctgctgagaa tatagaagtc gagccaaggc cgttgcttgt
     5701 tgttatttgc cacaatcaaa agggtatcga tgcgtgtcga caaaagatcg aagactcagt
     5761 ccgagatttg gcgctgggcg tatatttttt tggaaaaggc ggcgatttga atatttctaa
     5821 aaaaaatcac aaaaataagg cgctatttcc cttatgaacc tcatcatcca ccgcggcacg
     5881 catgagatcg gcggaacgtg tgtcgagatc gcgacagacg gcgggacccg gatcatcatc
     5941 gatctcggga tgccgctttc ggacccgcgc gacaagacga agaagctcaa gaatttttcc
     6001 cttaccggga agacggttcc tgaactgatc gggatgggta tcttgccaaa ggtctccggc
     6061 ctctactggg gtgtcggcga cgaaaagcca gtcaatgcga tcttcctttc ccatccccac
     6121 caggaccatt acggcctatt tccctatgtc cggaaggaca tcccggtcta tcttggcgaa
     6181 gacacggaca agatacttcg agcctcggag gtatttctcg gcgacaggtt cggtgtccat
     6241 gataaaaagg tcttgctgaa agaccggacc ccgccggatc ccgttgaaga tatcaaagtt
     6301 actccgttcc ttgtagatca ttcggcttac ggtgccatgg cctttctcat tgaagcggat
     6361 ggcaaaaggg tcttttattc gggggacttt cgcgggcacg gtcgcaaagc caagctgttt
     6421 gaaaaactga tcgccgatcc gcctccggat atcgacgcgc ttcttatgga aggaaccatg
     6481 atgggaaggc aggccgaaga tacgcagacc gaggacgagc tggaactcgt gattgcggat
     6541 gaagcgcggg tccatgaagg gatgaagctc tttatctgct ccggccagaa catcgaccgg
     6601 cttgttactt tttacagggc gacgaaacgg tcaaatgcca ctttcgtgct cgatctctat
     6661 gccgtgaata tcctgcagga gatcaacgac cgcgggacgc ttccggtgcc gtcaccggga
     6721 ttcaaagatg ttaaggtcct ttttacgaaa catttcatga aacggctcct gcattacaaa
     6781 aaagaggatt ggttcacgcg gtggcggccg cacgagatca gtccggccca gctccagcgg
     6841 gcaggaaagc ggtgttttgt gatgttcagg gacagctcgg tccacgagct gaagggcatc
     6901 atcccgaagg attccgtgat gttctattcc atgtggagcc aatacaccct cgagccggcg
     6961 ttcgagaaaa ccctcacttt tcttgatgaa aacaaggtcg cgttcaaaga agtccacacc
     7021 agcggacatg cgatcctttc agatctgaaa cgcttggccg ggaagatgaa acccggtatt
     7081 attatcccga tccacaccga gcgtccggaa gagtaccgtc ggcattttgg cggcaaagtt
     7141 caatgcttgc ttgatggtga agcttttaat atttgaaatg cataaactgg ttagataatc
     7201 agcgataaga atcgcaaaag gtgggtatga atatagaaca ggtaaaagat gttttaactg
     7261 aatatggtcg gatagagaac aaccttcaag gactggtcgc atctggagag aaacacaaaa
     7321 aaacaagtaa tactctgata gccgaattat ttttcaggcg ggccctggag ttagagccag
     7381 aaaatacaag agcaa
//
LOCUS       DAOSIO010000067         7296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_9335_length_7296_cov_3.935092, whole genome shotgun sequence.
ACCESSION   DAOSIO010000067 DAOSIO010000000
VERSION     DAOSIO010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 7296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7296
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9335_length_7296_cov_3.935092"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="PK590_03715"
     CDS             <1..1309
                     /locus_tag="PK590_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOG23735.1"
                     /translation="LMAVDAVVMVIDAGKGIENQTKKLFEICRHRGIPIFTFMNKLDR
                     PIRDPLSLIDELETVLGIRAYPVNWPLGSGRDFRGVYDRLQNKVHLFQKVPGGVYRAP
                     VSTHGFSDPEVKKMVDEQEYRQAAEALELLDAAHTEFSPEATLKGETTPVFFGSAANN
                     FGVQLLLDGFIKYSSGPAPRKSGERTIDVATEDFSGFIFKIQTNMNPLHRDRIAFLRV
                     CSGKFFRDMDVYHTRTGKKIRITGSHTLLGQSRETVDEAYPGDIIGFAVKSDFRVGDT
                     ISEDPSIVFNAIPRFAPECFAQIYNPSPAKYKPFRKGLEHLLAEDIVQVFSWIKPANP
                     NTALLGAVGPLQYEVLQYRLREEYGAESRLETLPWKVLRWVESGLNDSEMGNSLPYGV
                     ALARDEQGRRVLLFLSEWILNHFQEDHPDFTLFVSPRENRISL"
     gene            1436..2326
                     /locus_tag="PK590_03720"
     CDS             1436..2326
                     /locus_tag="PK590_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010041833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG23736.1"
                     /translation="MNQSLSLKRIRIGHSPDPDDAFMFYALAHEKIDMRGFEVEHVIE
                     DIESLNQRARKGELEVTAVSCHAYAYLADRYAVMRSGASVGDHYGPILVRRKADGEPR
                     EKDSVPRVAVPGQLTTAYLIMRLYEKLECQSPTAVLRAPSRAIFTPFDKIFDSVKSGK
                     SDYGLVIHEGQITYEKHGLEKALDLGSWWCERTRLPLPLGVDIIRKDLGPEVMRSFAA
                     LFKESIQYALKHREEALKYASQFGRGITPELNDRFVGMYVNDYTVELGEKGEAGFREM
                     MERAFSAGILPKKIHLEFIR"
     gene            2415..3014
                     /locus_tag="PK590_03725"
     CDS             2415..3014
                     /locus_tag="PK590_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23737.1"
                     /translation="MANDELRDKTLDVARRHKASWIELGQYLFSISKNKLFKEWGYLS
                     FEAYCVKELKIREATASKLLKSYMFLEKEEPRMVRPEYVAEEEPKKIPDYESVNLLRL
                     AKQNQHIPVQEFAELRHDVLDEAKDFRDVRAKVKTIVQEHKAKDTSESKEVKRNSVIK
                     RLIGFLNGARTQLESENLVPDYVIKQIETLASRLEDQLH"
     gene            3065..3331
                     /locus_tag="PK590_03730"
     CDS             3065..3331
                     /locus_tag="PK590_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23738.1"
                     /translation="MELWQYVGAIAGFCMPLFNIPLIFRIVKRKSAGDLSLFWLFGVW
                     TCILLMSPAALMSQDIAFRLFGWTNLIFFTVVVVVAAKYHKGQS"
     gene            complement(3463..4587)
                     /locus_tag="PK590_03735"
     CDS             complement(3463..4587)
                     /locus_tag="PK590_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOG23739.1"
                     /translation="MTKENLLIIGSSERNPDLYYATRFAAPDPFVFVQIREKKYILIS
                     DLEIDRARKQARVHAVLSTTTLAGKFREKHKRHASLSDLVIFFLREKKVRRLTVPQDF
                     PYLYALSLKRAGIRLDCHPGIFFKERAIKTPEEIRSVIQALRATENAARAAISLLKKA
                     TIRKNRLFYRNTPLTSESIRKLILAVLMEHDCAGENTIVSCGRHSVDPHHIGTGPLFA
                     NQPILFDIFPRNNKTLYYADFSRTVVKGKASPKLKKMFAAVKEGQAIAMRMIRPGVSG
                     RAIHNAIQSYFERLGFRTGLQAGRMQGFFHSTGHGLGLEIHEPPRIGGGDDILKEGHL
                     VTVEPGLYYKDAGGIRLEDLALVTRTGCKNLTQFPKILEI"
     gene            4852..4938
                     /locus_tag="PK590_03740"
     tRNA            4852..4938
                     /locus_tag="PK590_03740"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4886..4888,aa:Ser,seq:cga)
     gene            4950..6278
                     /locus_tag="PK590_03745"
     CDS             4950..6278
                     /locus_tag="PK590_03745"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG23740.1"
                     /translation="MTIKGKLIVFCFCLFLTPFAFPSFLRAEIIQLKNGNAMETKILR
                     EDDEFVTVEAVGGKVKIPKRDISKIWRGSKEELVEISAKRVFFTKGMELYKDGRFRES
                     AESFKQSLGPAAGNAIIYANLGSAYASAGEFQKAETSFLESLKQKPDHPDILLNLARL
                     YEAQKEYQKAVFYYLKTLNRSATDMSAQRSLAYCYYMNGDYLKAAETFGVLGEKNDIV
                     SRCNSAAAYIQAGELDRAEAILNEMAEDPFPVPRVYLNMGNLCFLKGDLVRAEQYYRQ
                     GLQRDPKTAEIHQMLGDLYLRLEQPELARQHFKEVLEKDPKNGWAMAGLAQALIKAEE
                     FEKAGDLCHQLAREHAGDSRVLNRVGLMYLKIGKPALALEIFNTLITRDEKDARAHSN
                     AGLAYVMRNDADNALKAWTRALELEPGLESALQNKKLLEEALSGESMDVQ"
     gene            6268..>7296
                     /locus_tag="PK590_03750"
     CDS             6268..>7296
                     /locus_tag="PK590_03750"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOG23741.1"
                     /translation="MSSKKGFTLLEILITAVIIIVLAALVLPRVLHQVRKAEEAEAIV
                     NLGAIRRAQLDLHGISGRFVEAADEKGIQAALGLSISNLIYDFKIIDADEDNFLGVAT
                     PIDALENWLEEIKIDKDGFVGYPGYGDGGGSSGGSGGGSSGGGSGSGGGSSGSGSGGG
                     SGSSGGSSGGCAGTRIDNVFYQATVKPVFNGYAANVQVVLDTLALMTDPLTADGMVTG
                     KDLVEWLQRNEIPVAFGSIDSWGLHYSSWGRSGGIPWIASEIKISTDLQSNKYACAAT
                     LAHEVLHATWWLDDYGKYFWLGTKAQPDYGMPTPTGEVRSKSSVDQEYNAFVTGSEIW
                     SWFKRSGYD"
BASE COUNT         1768 a         1621 c         2092 g         1815 t
ORIGIN      
        1 cctgatggcg gtggacgcgg tggtgatggt gattgatgcc ggaaaaggga tcgagaatca
       61 aacaaaaaaa ctttttgaga tttgccggca ccgggggatt ccgattttta cttttatgaa
      121 taaactggac cggccaatcc gtgaccctct gtcattgatc gatgagctgg agacggtgtt
      181 ggggattcgc gcctatcccg tgaactggcc cctcgggagc ggcagggatt ttcggggtgt
      241 ttatgaccgg cttcaaaaca aggtccacct ttttcagaaa gttccgggag gggtttaccg
      301 ggcgccggtt tcgacgcacg gcttttcgga tcccgaggtt aaaaagatgg tggacgaaca
      361 ggaatatcgc caggcggcgg aggcgttgga attgttggat gcggcccaca cggaattttc
      421 gcccgaagcg acgctgaaag gcgagaccac gcctgttttt ttcggcagtg cggccaataa
      481 tttcggagtc cagcttcttc tggacgggtt tatcaaatat tcgtcaggcc ccgcgccgcg
      541 gaagtcgggg gagcggacga ttgatgtcgc caccgaagat ttttccggtt ttattttcaa
      601 gatccagacc aatatgaacc cgcttcaccg cgaccggatc gcgtttctca gagtttgttc
      661 aggaaaattt ttccgggaca tggatgttta tcatacccga acaggaaaaa aaatcaggat
      721 aacgggatcg cacacgcttc ttggtcagag ccgggaaacg gtcgatgagg cgtatccggg
      781 cgatattatc ggatttgcgg tgaaatcgga tttccgggtg ggagacacaa tcagtgaaga
      841 tccctccatt gtttttaacg ccattccgag attcgcgccc gaatgttttg cccagattta
      901 taatccctcg ccggccaagt acaagccgtt ccggaaaggg cttgagcatt tactggctga
      961 agacattgtc caggtattca gctggatcaa gccggccaat cccaatacgg cgcttttggg
     1021 agcggtcggg cctttgcaat atgaggtgct tcaataccgg ctcagggagg agtatggcgc
     1081 tgagtcccgg ctcgagaccc ttccctggaa agtccttcgg tgggtcgaat ccggactgaa
     1141 tgattctgaa atggggaatt ccctgccgta tggcgtggcg ctggcccggg acgaacaagg
     1201 gagaagggtt ttgctttttc tgagcgagtg gattttgaat cattttcagg aagatcatcc
     1261 ggattttacg ctctttgttt ctccgcggga gaaccgtatt tccctatgat caggggaatg
     1321 ctttggcagg attcgcggat gtcccgctta ctggagcgag catgattttc tccgcggatt
     1381 ttaggcaagt atgatttgaa aggaaccaac gcataataaa gacggaggtt ttaaaatgaa
     1441 tcagagttta tctttaaagc gtatccggat cggacacagc ccggaccctg atgacgcgtt
     1501 tatgttttac gcgcttgctc atgagaagat cgatatgagg ggatttgaag tcgagcatgt
     1561 gattgaggat attgagtccc tgaaccagcg cgcccgaaag ggagagcttg aggtcacggc
     1621 ggtttcttgt cacgcttacg cgtatctggc ggaccggtat gccgtgatgc gttccggggc
     1681 gagcgtgggg gatcattacg ggccgattct cgtacggcga aaagcagacg gtgaaccgcg
     1741 tgaaaaggat tccgtaccca gggttgctgt tccgggccag ttgaccacgg cctatttaat
     1801 catgcgactt tatgaaaaac tcgaatgcca atcaccgacc gccgtacttc gcgcgccgtc
     1861 ccgggcgatt tttactccct tcgataagat ttttgattcc gtcaaaagcg gaaaatcaga
     1921 ttacgggctt gttattcatg aaggacagat tacctacgaa aaacacggtc tcgaaaaagc
     1981 gctggatttg ggatcgtggt ggtgcgaacg gacacgactt ccgctacctc ttggggtgga
     2041 catcatccgt aaagaccttg ggccggaggt aatgaggtct tttgccgccc ttttcaagga
     2101 gagtattcaa tatgccttga aacaccgcga agaagcctta aaatacgcga gccaatttgg
     2161 ccgcgggatt actccggaat tgaacgatcg gtttgtcggg atgtacgtga atgattacac
     2221 ggtcgagctg ggtgaaaagg gagaagctgg tttccgggag atgatggagc gagccttttc
     2281 agcagggatc cttcccaaaa agatccattt agaattcata aggtaaaatt cataaaaacc
     2341 ggtagggctt atagcgcgtg gtggaaaagc tttcacgttg cgcgccggcc gcccttcccc
     2401 ctatgaggag tatcatggcg aacgatgagt tgcgagataa aacgctggat gtggcgcggc
     2461 ggcacaaggc gtcgtggatt gagttggggc agtatctttt ttctatttcc aaaaacaagc
     2521 ttttcaaaga atggggttat ctgtcctttg aggcctattg cgtcaaggaa ctgaaaattc
     2581 gtgaggccac cgcctccaaa cttctgaagt cctacatgtt tctggagaag gaagagcccc
     2641 gaatggtgag acctgagtac gtggctgaag aggagccgaa aaaaattccg gactacgagt
     2701 ccgtgaatct cctgcgtctc gccaagcaaa atcagcatat cccggtccag gaatttgcgg
     2761 aacttcggca tgatgttctg gatgaagcca aggatttcag ggatgttcgc gcgaaggtca
     2821 agacgattgt ccaggagcat aaggctaagg acacgtccga gtcgaaggaa gtgaaacgaa
     2881 attccgtcat caaacgcctg atcggatttt tgaacggcgc caggacccag cttgaatctg
     2941 aaaatctggt gccggattat gtcatcaaac agatcgaaac cctggcttcc aggcttgagg
     3001 atcaactgca ttagaatcgg ctgacgggct ttcagcgttt cggaaaaaac aggagagcaa
     3061 ctcaatggaa ctatggcagt atgttggcgc gattgccgga ttttgcatgc cgctttttaa
     3121 cattccgctc atctttcgta tcgtgaaacg aaagtcagcc ggggatctga gtcttttttg
     3181 gctgtttggc gtatggacat gtatccttct tatgagtccc gcggctttga tgtctcagga
     3241 catcgccttt cgtctctttg gatggaccaa tctcattttt tttacggtcg tggtggtcgt
     3301 ggcggcaaaa tatcacaagg gtcaatcctg aaattctccc cggtgatatt ccggaacggt
     3361 acgatttcct ttctgatttt aaaaacgttc gtcacgtgga ttggattgtg gtgacggagc
     3421 gtggcggaat gattgaggaa aggatccgga aaaatctttg gatcagatct cgagaatttt
     3481 ggggaattgg gtgagatttt tacagcctgt ccgggtgacc agcgccaaat cttccagccg
     3541 gatcccgccc gcgtctttat agtaaagccc cggctccacg gtgaccagat gcccctcctt
     3601 caggatatca tcacctccgc cgattcgcgg aggctcatga atttccagcc cgagcccgtg
     3661 gccggtgcta tggaaaaatc cctgcatgcg gccggcttgt aatcctgtcc ggaatccaag
     3721 gcgctcgaag taagactgaa tggcgttgtg aatggcccgg cccgagacgc ccggacggat
     3781 catacgcatg gcgatggcct gtccttcctt gacggcggcg aacatctttt tcagtttagg
     3841 ggaggctttt cccttcacga ccgttctgga gaaatcagcg taatagaggg ttttgttgtt
     3901 gcgggggaaa atatcaaaga ggatcggttg attcgcgaac agcgggcccg ttcctatgtg
     3961 gtggggatca acggagtggc ggccgcatga cacaatagtg ttttctccgg cgcagtcgtg
     4021 ttccatcagg acggcaagaa ttagttttcg gatggattca ctggtcaatg gcgtgttccg
     4081 gtaaaagagc cggtttttcc ggatcgtcgc cttttttaaa agcgagatag cggcgcgggc
     4141 ggcgttttcg gtggctcgca gggcctgaat gaccgaccgg atttcctcgg gtgttttaat
     4201 cgcgcgttcc ttgaagaaaa tcccgggatg gcagtccaga cggatcccgg cgcgtttcag
     4261 ggaaagggca tagagatagg gaaaatcttg gggaaccgtg aggcggcgca cttttttttc
     4321 acggaggaaa aaaatgacca agtcggacag ggaggcgtgg cgcttgtgtt tttcccggaa
     4381 tttccccgct agggtcgtgg tgctgagcac ggcgtgaaca cgtgcctgct ttctggcacg
     4441 gtcgatctcc aggtcgctga tcaggatgta ttttttttct ctgatttgca cgaagacaaa
     4501 cgggtcggga gcggcaaacc gcgtcgcgta gtaaagatcg ggatttcgtt cactgcttcc
     4561 gatgatcagg aggttttctt tcgtcatggc gggaattata tcatggcgca aaacaaaagg
     4621 ataagcgtgg tgaattaaaa tgaatctaaa accagttccc cccgtcgcct cggtgccctg
     4681 gctcctgtct tgaccaactt gcggaaaaac aatagacttc agatcactag ggcttgtttc
     4741 gcgaatggcc gtccatgaca ctcgcgtgag cgcgtgtcat ggttttttgc cccgaaaatt
     4801 ttagctttga aagaaataat ctcgggcgcg taaattcaaa aaatgatttt tggagaggtg
     4861 gcagagtggt cgaatgcggc agtctcgaaa actgttatac gcgcaagcgt atcgggagtt
     4921 cgaatctccc cctctccgat ggtttttaca tgaccataaa aggaaaactg attgtttttt
     4981 gtttctgcct gtttcttacc ccttttgcct ttccctcttt tttgcgcgcc gagatcattc
     5041 agcttaaaaa cggcaatgcc atggaaacaa aaattctgag agaagacgat gagtttgtca
     5101 cggtggaggc ggtcggcggc aaggtcaaaa ttcccaaaag ggacatctcg aaaatatggc
     5161 gaggatcgaa agaggagctc gtcgaaattt ccgcaaagcg ggtttttttc accaaaggga
     5221 tggagcttta taaagatgga cggtttcggg aatccgcgga atcttttaag caatcactcg
     5281 ggcccgccgc tggcaacgcg atcatctacg ccaatctcgg aagcgcttac gcttccgcgg
     5341 gtgaatttca aaaagccgaa accagttttc tggagtctct gaagcaaaaa cccgaccatc
     5401 cggacatcct gctgaacctt gcccggttgt atgaagctca aaaagaatat caaaaggccg
     5461 tgttttatta cttaaaaaca ctgaatcgaa gcgccacaga tatgagcgca cagcgaagtt
     5521 tggcttactg ctattacatg aacggggatt atttgaaagc ggcggaaacg tttggggtcc
     5581 ttggagaaaa aaatgatatt gtctcccggt gtaacagcgc ggccgcttac atccaggcgg
     5641 gggaactgga cagggccgaa gccattctta acgaaatggc cgaggacccc tttccggtgc
     5701 cacgcgtgta tctgaatatg ggaaatctct gttttttaaa gggggatctg gtacgggcgg
     5761 agcaatatta ccggcaagga ttgcaacgag atccgaaaac cgcggagatc caccagatgc
     5821 tgggtgacct gtatttgcgt ctcgaacagc cggaactggc acgacaacat tttaaggaag
     5881 ttcttgagaa agacccgaag aatggctggg ctatggcagg tttggcgcag gcgcttatta
     5941 aggctgaaga atttgaaaaa gcgggagatc tgtgccatca attagcgagg gagcatgctg
     6001 gtgattcccg cgtgttaaat cgtgtgggtt tgatgtatct gaaaataggt aagccggccc
     6061 tggcgcttga gatttttaac acgctgatta cccgggatga aaaagacgcc agggcgcatt
     6121 caaacgccgg gcttgcttat gtcatgagga atgatgccga taatgcccta aaggcatgga
     6181 cccgggcgct ggaactggag cccgggctag aatctgccct gcaaaacaaa aaacttctgg
     6241 aagaagcttt aagcggagaa tcgatggatg tccagtaaaa agggatttac cctgcttgaa
     6301 attcttataa cggcagtcat tattatcgtc ctggccgctt tagtgcttcc ccgggtactg
     6361 catcaggtcc gaaaagcgga ggaagcagag gctatcgtca accttggcgc gattcggcgc
     6421 gctcaattgg acctccacgg aatatcgggg cggtttgtcg aagcggcgga tgaaaagggc
     6481 attcaggcgg ctctcggcct ttccatttcg aacctgatct atgatttcaa gattattgat
     6541 gcggacgaag ataattttct gggcgttgcc acgccgattg atgcgcttga aaattggctc
     6601 gaagagatca agatcgataa agatggattt gtcggatatc ccggatatgg tgatggagga
     6661 ggcagtagtg gcggaagcgg tggcggcagt agtggcggag gatccggttc cggtggcggc
     6721 tcaagcggta gtggaagcgg aggtgggagt ggttctagcg gcgggtcttc gggaggatgt
     6781 gccggaacta gaatagacaa cgttttttac caggctacgg tcaagcctgt atttaatggt
     6841 tatgccgcaa atgtacaagt agttttagac acacttgcgt tgatgacaga tcctttaaca
     6901 gctgatggaa tggttacggg gaaagatctt gtggagtggt tgcaaagaaa tgaaattcct
     6961 gttgcattcg gaagcattga ttcctggggt cttcattatt cttcatgggg ccgttctggt
     7021 ggcataccgt ggattgcaag tgaaattaaa atcagcacgg atttgcagtc taacaagtat
     7081 gcctgtgcag cgacgcttgc ccatgaggta ttacatgcca cctggtggct tgatgattat
     7141 ggaaaatact tttggcttgg gacaaaagct caacctgatt atgggatgcc caccccgact
     7201 ggcgaggtta gatccaagag ttccgtggat caggagtaca atgcctttgt gaccgggtcg
     7261 gaaatttgga gctggtttaa acggagtggt tatgat
//
LOCUS       DAOSIO010000068         7198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_9466_length_7198_cov_3.749265, whole genome shotgun sequence.
ACCESSION   DAOSIO010000068 DAOSIO010000000
VERSION     DAOSIO010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9466_length_7198_cov_3.749265"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /locus_tag="PK590_03755"
     CDS             <1..1144
                     /locus_tag="PK590_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOG23742.1"
                     /translation="GILITALMFVAFLISGTGLQNIMPHASIFAFGLVAYGFLCMGPV
                     NIAVDSYGPVTDNAQSIFELAQTESIPGIESEIERDFGFKPDFASGKHYLEANDSAGN
                     TFKATAKPVLIGTAVAGATTMIFSIILLLQEHLQTTAIAAAAGGFVPTNIGEILLHQQ
                     LSLTAAPVLLGFICGGAVIYWFSGASIQAVATGAYSAVEYIKKNMNLEMKEAQREDSI
                     KVVQICTEYAQKGMWNIFLGLLSLTLAFAMFDPYFFIAYLLAIAVFGLFQAMFMANAG
                     GAWDNAKKYVEVDLGEKNTPLHAATVIGDTVGDPFKDTTSVSLNPIIKFSTLFGMLAV
                     EIAIKMNPATTTVSGAVLFVAGLFFVWKSFYKMRIPKLEITAKTPS"
     gene            complement(1284..2207)
                     /locus_tag="PK590_03760"
     CDS             complement(1284..2207)
                     /locus_tag="PK590_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOG23743.1"
                     /translation="MLAWSIFGVVVIALLALDLGVFHRKAHEVKMKEALLWSAVWIAV
                     ALIFNVGVYWMKGKELAIQFLAGYLLEKSLSVDNLFVFLLIFNYFHLPARYQHLILFW
                     GIIGALVARAIFIVCGLGLLHYFSWMIYVFGLILIWTGVKLAFEKNKKVEPEKNVFVK
                     VFRRFFPLTQDYHEGKFFVRLNGVLHATPLIVILLVVESTDILFAVDSIPAILAISRD
                     PFIVYTSNVFAILGLRALYFALAQLMKLFHYLHYGLSLILVLIGVKMIASHFFHIPTV
                     ATLGTILLVLVFSVVASILWPQSSKEARANS"
     regulatory      complement(2318..2455)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(2456..2683)
                     /locus_tag="PK590_03765"
     CDS             complement(2456..2683)
                     /locus_tag="PK590_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23744.1"
                     /translation="MKKVIVVMLALALFAAQGIACAEDVVADVQAQADDMMEETLDAE
                     DLIMDESDEMMLVEETEEVVPVEAEGQKGLI"
     gene            complement(2926..3600)
                     /locus_tag="PK590_03770"
     CDS             complement(2926..3600)
                     /locus_tag="PK590_03770"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG23745.1"
                     /translation="MSFKALVDVGYWPPDMVSDDSSIFWKAYIHFDGDYRVVPIPVTV
                     SMDVVNSGSWWRTCVSLYEQKRRWAWGVENFPLVIRGFLKNGKISLFDKCRHAFKLLE
                     GHVTWAAMPFLLTFAGWFPAILAGKEFSGTVLYFNAGRVTQTIFGLSSLVVIGTVIFS
                     MWLLPPKRKRFNLFWTFVHSLEWLLVPVILIFFSALPALDAQTRLMMGKRLSFMSSDK
                     KRGRLP"
     gene            complement(3597..4571)
                     /locus_tag="PK590_03775"
     CDS             complement(3597..4571)
                     /locus_tag="PK590_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23746.1"
                     /translation="MRFLYSAQNLSGSDKVLQRVLEIIPGTLSWVLIVGLFLFAFWQP
                     VAVAIIIIVFMLSWVMRLFYYLIFLGLSYARIRYEKGTDWISRVRGLDRFEDYAEQVR
                     REKPAGSIGARLSAWSLRKDLEEIRRRNAFPPPSDEIYHIILIPVIKESREVLEGSLE
                     GLLQNREFLNRMLVVVALESRAEASIKSGVRELKERYASQFFGFQVVEHPDGLPGESR
                     VKGANISFAAKVAREFLRKRNINFENVIISCLDADTIVGADYFSCLTYAFMVCPDRHR
                     ASFQPIPVYHNNIWEVPGFARVVETGSSFFSLLKRLIPKNLSLFPATA"
     gene            complement(4568..6025)
                     /locus_tag="PK590_03780"
     CDS             complement(4568..6025)
                     /locus_tag="PK590_03780"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOG23747.1"
                     /translation="MLLLRKILFYVFLIIYLVICPIVILYSFGYIYVPTPEEGIVKTG
                     LIHLESLPPGAEVRLDQKLMPDHTPCTLSELVPGRYSVTVSLENYRHWFRQVTVEPGK
                     AAVFDRILLIPEHLRCSVLKDHPFETIVNIPGTRFLLLKSGQNAGGLTVYDWKNNSFK
                     KLISQGTPYAGEATERIFTVKESPHVLVLTQAKETLRYLWCDLREENIVISEITELFR
                     DTIPDEVKWEGTYPRFLFAFHKGRLDRLDLDESKVDQNFTERIKGFGIHKGKVYTLRN
                     DSLARMPLDANGSGPDIVERGRFLSRIFERDDTFRMDFLRADKVFFIGKKQTLFANRL
                     PYHFVEKGFVDYWPDDDFKMALIWTSKEIGLLNFEKRALAGALFERGAEVDWFFHQGS
                     DIRQVFFAYDTSHVIFNDGETVFLFEVDGKGKRPVKVTEILKGSGLFYSGKKGSLYYL
                     DPETRYFMLLEILPEEKYLERMFQEIEKTRRHQKV"
     gene            complement(6220..>7198)
                     /locus_tag="PK590_03785"
     CDS             complement(6220..>7198)
                     /locus_tag="PK590_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23748.1"
                     /translation="DDWRSVLTRWALINEKHKTLIRGVAEPRRNTEYFIYQAILGAWP
                     ESGTNAQSGPAFTDRIWEYVLKSIREAKTHTNWIHPKREYEESVRKFVYGILSPGKGN
                     RFLKNFRLFHEKVSWFAKLNSLSALVLKIGSPGVVDVYQGTEFWNLFLADPDNRRPVD
                     FEERKGALQKIMKGSKIRGLDGDIASLLTGKKSERVKMQVLALGLRLRRSEPGIFLEG
                     DYVPLRVEGERERNVVAFLRREGDRFVIFAAGRFFTEISGGPGQLPVGPAAWKTTRIL
                     LPAGLACRETLKDILTDREVPVGVDGELKSLRVSDVFGRLSVGILMNGS"
BASE COUNT         1976 a         1971 c         1592 g         1659 t
ORIGIN      
        1 agggatcctc atcacggccc tgatgttcgt ggctttcctg atctccggaa ccggacttca
       61 aaatatcatg ccccatgctt caatttttgc ttttggtctc gtcgcttatg gattcctctg
      121 tatgggaccc gtcaatatcg cggtggattc ctatggcccg gtaaccgata acgctcaatc
      181 catttttgaa ttggcccaga ccgagtcaat ccccggcatt gaaagtgaga tcgagagaga
      241 tttcggcttc aagcctgatt tcgcaagcgg caaacattat ctggaagcca atgactccgc
      301 cggaaacacc ttcaaggcca cggcgaaacc cgtgctgatc ggcaccgctg tcgcgggcgc
      361 taccaccatg attttctcga tcatcctgct tttgcaggag catctgcaaa cgaccgcgat
      421 cgccgcggcc gcgggaggtt ttgtaccgac caatatcggg gaaatcctgc tccaccagca
      481 gttatccctc acggccgctc ctgttctgct tgggttcatc tgcggaggag ccgttattta
      541 ctggttctcc ggagcctcca tccaggcagt cgctacggga gcctattcag cggtggaata
      601 cattaagaag aacatgaacc tcgagatgaa agaagctcaa cgtgaggatt cgatcaaggt
      661 ggtgcaaatt tgtacggagt atgcccagaa aggaatgtgg aatatcttcc tcggcctgct
      721 ttccctcacc ctcgctttcg cgatgttcga tccctatttc ttcatcgctt atcttttggc
      781 catcgctgtt ttcgggctct tccaggccat gttcatggca aatgccggcg gtgcctggga
      841 caatgccaaa aaatatgttg aagttgatct tggcgagaag aacactcccc tgcacgcggc
      901 cacagtcatc ggagataccg tcggagaccc gtttaaagat acgacgtcgg tctcgttaaa
      961 cccgatcatc aaattctcga cccttttcgg catgcttgcc gtcgaaatcg cgatcaagat
     1021 gaaccctgcg acaaccacgg ttagcggggc cgtccttttt gtagcgggac tattttttgt
     1081 gtggaagtcc ttttacaaga tgcgtatccc gaaactggaa atcacggcga aaaccccaag
     1141 ctgatttttt ccgagatttc ggaagacgat caagaaaaaa gccccgtcga acgacggggc
     1201 tttttttgtg tttggatttc accggttaca tcagtcgtga tttcatttcg taacgacccg
     1261 gctcccggcc caaatcagca aactcaggaa ttcgctctcg cctcttttga cgactgaggc
     1321 caaagaatcg aagccaccac tgaaaagaca aggaccagca gaatggtccc gagagtcgct
     1381 acggtcggaa tatgaaaaaa gtgcgacgcg atcattttga caccaatcag gaccaaaatc
     1441 agcgaaagac cgtaatggag ataatgaaac agcttcatga gctgggccag cgcgaaataa
     1501 agcgcccgaa gtccaagtat cgcgaaaaca tttgaggtgt aaacgatgaa cggatctctg
     1561 gaaatcgcaa gaatggcggg gattgaatca accgcgaaca aaatgtccgt ggattccacg
     1621 acaagcaaaa tgacaatcag cggcgtggca tgaaggacgc cgtttaatct gacaaaaaat
     1681 tttccttcgt gatagtcctg ggttagcgga aaaaagcggc gaaacacttt aacgaaaacg
     1741 tttttttccg gttcgacttt cttatttttc tcaaaagcca gtttcacgcc cgtccatatg
     1801 aggatcagcc cgaagacgta aatcatccag gaaaaatagt gcaataaacc aagaccgcaa
     1861 acaataaaga tggctcgcgc gacaagcgcg ccgataatcc cccagaaaag gatcagatgc
     1921 tgataccggg cgggaaggtg aaaatagttg aagatcagga gaaacacaaa aaggttatcg
     1981 acgctcagtg acttctcgag aagataaccc gccaaaaact ggatcgcgag ctctttgccc
     2041 ttcatccaat aaacaccgac gttaaagatc aacgccacgg caatccagac ggcgctccag
     2101 agaagcgcct ccttcatttt gacttcatga gccttgcggt gaaaaacccc gagatctagg
     2161 gcaaggagag caatgacaac cacgccaaaa attgaccagg ccagcatatc cgacattatc
     2221 tttctcgatg aaataaaaag ttgattcaaa caaaaaattt gttaacgaac agaaaatcag
     2281 gggccagccc tcacggactg gcccctgcta gcctaataaa accgatctgc aacgcctacg
     2341 gaattagttc actggttcga gccgcagatt agcccgtctc accttcctgc cggcaagatt
     2401 aaccctttca acatctctcc cgttctcaaa aaacgagatt cggcgatccc gtgtcttaga
     2461 tcagcccctt ctgaccttcg gcctcgacgg gaaccacttc ctcggtctcc tctaccaaca
     2521 tcatctcatc gctttcgtcc atgatgagat cttccgcgtc aagagtttcc tccatcatat
     2581 catccgcttg agcctggaca tcggcaacaa cgtcctcggc gcaggcaatt ccctgagcgg
     2641 caaaaagcgc cagggcgagc atcacaacta tcactttctt catgtaagga tctcctttca
     2701 agatctaccg tgcaatccgc cggaccattc cgggaaaaaa ctattccaga tttgcatccc
     2761 aagctccccg tggacacgac atctgggaag catggccggt tcctcttaat aagattttga
     2821 aacgctgttc aaatcttact gattaaagtg agtgtaattg gggaattgtg ccatgtcaac
     2881 aggatgtttg taaatgctat acgatgctat gagaggtagc ggagctcagg gcagtctgcc
     2941 acgtttttta tccgatgaca taaaactcag acgttttccc atcatcaatc gtgtctgggc
     3001 gtccagcgcc ggcagggcgc taaaaaaaat cagaatgact ggaaccaata accactccag
     3061 actatgaaca aacgtccaga acagattgaa ccgcttgcgt ttgggtggca aaagccacat
     3121 gctgaaaatg acggtcccga taaccaccag agatgagagc ccaaaaatag tctgggtaac
     3181 gcggccagcg ttaaaataga gcaccgtacc cgaaaattcc ttaccggcaa gaatggcggg
     3241 aaaccatccg gcaaacgtca gcaaaaacgg catggccgcc catgtcacgt gcccttcaag
     3301 taatttgaac gcgtggcggc atttgtcgaa gagagagatt ttgccattct ttaaaaatcc
     3361 ccggatcact aacggaaaat tttcgacccc ccaggcccat cgtcttttct gttcatataa
     3421 gctgacacag gttcgccacc aggaacctga attcaccaca tccatcgaaa ccgtgaccgg
     3481 aatgggaacc acccggtagt ccccgtcgaa atggatataa gccttccaga agatgctgga
     3541 atcgtcggaa accatgtctg gtggccaata cccaacgtcc accaacgctt taaaactcat
     3601 gctgtggctg gaaaaagtga caagtttttc gggattagtc gtttcaacaa gctgaaaaaa
     3661 gaagaacctg tttcgactac ccgggcaaaa cccggaacct cccaaatatt attgtgataa
     3721 accggaatag gttggaaact ggcgcgatgc cggtcgggac acaccatgaa cgcgtaggtc
     3781 aagcaggaaa aataatcggc cccgacaatc gtgtcggcat ccagacaaga gataatgaca
     3841 ttttcaaagt taatattcct tttcctcaga aactctctgg cgacctttgc ggcaaaggaa
     3901 atattggctc ctttcaccct cgattctccc ggcaatccat ccggatgctc caccacctga
     3961 aatccaaaaa attgagaggc atacctttct ttcaattccc gaacacctga cttaatcgaa
     4021 gcttcagccc gggattccag agccaccacc accagcatcc ggttcaaaaa ttcccggttt
     4081 tgcaatagtc cctcaagact tccttcaagg acctctcgcg attcttttat cacggggata
     4141 agaatgatgt ggtaaatctc atcagaaggc ggagggaaag cgttccggcg cctgatttcc
     4201 tcaagatcct tacggaggga ccaggcagag agccttgctc cgatactccc ggccggtttt
     4261 tcccggcgca cctgttcagc ataatcctca aaacggtcaa gcccccgaac tctcgaaatc
     4321 cagtccgtac ctttttcata gcgaattcgg gcgtacgaaa gtcccaaaaa aatcagatag
     4381 taaaaaagcc gcataaccca cgaaagcata aaaacaataa taatgatagc caccgccacc
     4441 ggctgccaga acgcgaacag aaaaagcccg acaatcaaaa cccagctcag ggtacccgga
     4501 ataatctcaa gaacccgctg gagaacttta tcggacccgc tcaggttctg ggctgaatac
     4561 aaaaacctca taccttttgg tgtctcctcg ttttttcaat ttcctggaac atccgttcaa
     4621 gatatttttc ttccggcaaa atctccagga gcataaagta ccgggtttca gggtctaggt
     4681 aatacaaact cccctttttc ccagagtaga acaggccgct tcctttcaaa atctcggtga
     4741 ctttgaccgg tcttttccct ttaccgtcaa cctcgaataa aaatacggtt tccccgtcat
     4801 tgaagatcac atgagaggta tcatacgcga aaaaaacctg acggatatcc gaaccttgat
     4861 ggaagaacca gtccacttca gcgcccctct caaacagcgc cccggccagc gcccgttttt
     4921 caaaattgag aagtccgatt tctttggacg tccagatcag ggccatttta aaatcatcgt
     4981 cgggccaata atccacaaat cctttttcga caaaatgata cggtagtcga ttggcgaaaa
     5041 gggtttgctt ctttccaata aaaaaaactt tgtccgccct taaaaagtcc attcgaaagg
     5101 tatcatccct ttcaaaaatt ctggaaagaa accttccccg ctccacaatg tccgggccag
     5161 aaccattcgc gtccaggggc atccgtgcca gagaatcgtt cctgagggtg taaactttcc
     5221 ccttgtgaat tccaaaaccc ttgatgcgtt cggtaaagtt ttgatcgact ttcgactcat
     5281 cgagatccaa acgatccagt cgccccttat gaaacgcgaa caaaaacctg ggataggtcc
     5341 cctcccattt gacttcatcg ggaatcgtat ctcggaaaag ctctgtgatt tccgaaatga
     5401 caatattctc ctcccggaga tcgcaccaca ggtagcggag ggtctctttc gcctgggtca
     5461 ggacaagcac gtgagggctc tctttgaccg taaaaattct ctccgtggct tcccccgcgt
     5521 aaggggtccc ttgagaaatc agttttttaa aactgttgtt tttccagtca tagaccgtca
     5581 gccctccggc attctgcccc gattttaaaa gtaaaaaccg ggtacccgga atgttgacaa
     5641 tcgtttcgaa tggatgatct ttcaaaacgg aacaccgaag atgttcgggg ataaggagaa
     5701 tccgatcgaa aacggccgcc tttccgggct caacggtcac ctgccgaaac cagtgccgat
     5761 aattctccaa actcactgtc acggaatatc gaccgggaac taactcactt agggtgcatg
     5821 gcgtgtgatc cggcatcaat ttttgatcaa ggcgaacctc cgctcccgga ggaagacttt
     5881 ccagatgaat aagtcccgtc ttgacaattc cctcctcagg cgtcggaaca tagatatagc
     5941 cgaacgagta gagaatcact atcgggcaaa tcaccagata gatgatcaaa aaaacataga
     6001 aaaggatttt acgaagaaga agcatggaga gcattatgcc ataccggaca gggttctttc
     6061 tctaagaaag aataaaacgg ccatattccc caaaactccg ctcgggatca ttgtcggcca
     6121 aaatccaaaa atttctcacc tcgatcaaaa tcagaatttc aggaaacggg gccgaaagga
     6181 aagttgtagc gtgattctgg ttttacgggg tcattcccat catgacccgt tcattaatat
     6241 ccccacgctc agccggccaa acacatccga cactcgcagg ctttttagtt ccccgtccac
     6301 tccgaccggc acttcccggt ccgtcagtat atctttcaac gtttcacggc acgccaaacc
     6361 cgccggcagg agaatccgcg tcgtcttcca agccgcgggt ccgacgggaa gctgtccggg
     6421 cccacccgat atttcggtaa aaaaacggcc cgcggcaaaa attacgaacc ggtctccctc
     6481 gcgccgcaaa aacgcgacca cattcctttc cctctcaccc tccaccctga ggggaacata
     6541 atccccttcc agaaaaattc cgggctccga cctccgcagt cttagcccca gagcaagcac
     6601 ctgcatcttg accctttctg atttttttcc ggtcagtagg gaagctatat ctccgtccaa
     6661 cccccgaatc tttgaaccct tcatgatctt ttgaagcgcg cctttacgct cctcgaaatc
     6721 aacggggcgg cgattatccg ggtcggcaag aaacaaattc cagaactcgg taccctgata
     6781 aacgtccacc acgcccggag acccgatttt aagaacgagc gccgacaagg aattcaactt
     6841 cgcgaaccag gacacttttt catggaaaag acgaaagttc ttaagaaagc ggttgccttt
     6901 cccggggctt aaaattccgt aaacaaattt acggacagat tcttcgtatt cccgcttcgg
     6961 atgaatccag ttcgtgtgcg ttttcgcttc ccggatcgat ttcagaacgt actcccagat
     7021 ccggtccgta aacgcgggac ccgattgcgc gttcgtcccg gattcaggcc aggcccccaa
     7081 aatcgcttga tagataaaat attccgtatt tcggcgaggc tccgccaccc cccggatcaa
     7141 ggtcttatgt ttttcattaa tgagcgccca ccgggttaat acgctccgcc agtcatcc
//
LOCUS       DAOSIO010000069         7138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_9558_length_7138_cov_3.137936, whole genome shotgun sequence.
ACCESSION   DAOSIO010000069 DAOSIO010000000
VERSION     DAOSIO010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7138
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9558_length_7138_cov_3.137936"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>7138)
                     /locus_tag="PK590_03790"
     CDS             complement(<1..>7138)
                     /locus_tag="PK590_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23749.1"
                     /translation="AAFGEAKQVLTGGRETWLFSNLSQSTALAMTQNFAGWSLVPHGL
                     LAEGLSLKSPEDWKRRVFELGDAGGKVRMLSSHNQDQNVYVFARADTKSGSTLPNTEP
                     KVVGTLGIINKGLIREIDSATSKPTGKWIYSGDALDSQALPITGFGVFDPLLGANGLT
                     VFSAEFIPGKVDATKATQTSSDGKWKLASTTYDETANTVLTITQFHASVGTNSVAAIQ
                     EDSLKPVSITRAENNALTARGFDNLTFPVAGFVTFSDADGTWSFTAQFTPQKDAVPTD
                     KAASLSEHWSYEFYSARGTRIAGADHVDSGDKASDVSANAVLRAGQNIVREMNTNTDA
                     TTISASSLGGIANMNIWIADSKTQRYLFFAQYDGSGNLSKHFSTDAVRHFKGQIAPGK
                     TFDIFGGVEGLKNLNSEELAAVKASLPAEALKGNGLGACTAGETGTVVWATSNLSEGL
                     TEADAVAFIRSFQYEVNLPQTNVLRVWDRVYNENDKIPEGFRAGDKVNAWETTLDERG
                     MAGDYLWRGVGSENRGGLLATQPDPKQRNEVYMIPTDRKTWENQKALNVIREKYDEVQ
                     GAGAFDKAFKNAKVLQGGYGLFFNGSPDGAMEKFLEGNFTQGKQFRDLMGQLSSFTLV
                     YGETDKPKQYQIQGDTAGLAKGKLEKTLYLINQIQDPATGQVTRTEGKVLVAPSDGVR
                     FGAVPPSALTFSSGFWLYPTWEQYKSAPTSPVESWMVKDFKVEHNGVVRDQSRGTTFD
                     ASSMLGAIRSSGFLGSDANFAREIEATVIVGGSAASTQVTEAILSDLLIPRYTTTVLN
                     VAGEGESRVETELLKFLGRANTKMDAPDTKFAFLGLGAYAADSAKGEAKKIYMIQNGK
                     LDTATRAVSRTAWEFTATMGAFEKLKDNEIRAANDTRTAANGSTINFGFFQGIDITGV
                     KKYVNGTEQMAGDIAPTTRELFLEGCRTFGQRELTSFYTNDANVSGGAWLSWQADRKF
                     NAAQNVTQFLIGRGANEGLYAFRGNVGKSPQTAAGKWQEVSSALNPSQISTAARFDGK
                     VGPKTTRGFQNKVVIHADGVLADNDPLRSALMAEFGKFGVSDYVVWTDANFNATWRAQ
                     GLMDVNGWFTHDTPLTGMGDYAGQSRVSFDKSALKPMNVDIELQNQKPDQPKLPGQLV
                     VKRDGLVTVWVEGKLYASGHLHKGRSAIFALLTKVDDPAQRFFSVSMNQGETFDAVRE
                     LPPSTINQNELPGGVGPNGEQLKGFSFGGDHFIQASAGNPNPPCRIDWFLSADVSAFE
                     AVYKSSPGVFDELAKLFGETTFSLVKNGQRIFSGFLYTESFDIAGNRIFFGRPEGGMG
                     DKVFVSERGGDKTYNLIETHKAATPIGDQREFDSVTQNTLTAFARKSILPNSQDKNVK
                     VRRFGFTNFEMIADEIDNFSLGMYQGLTDAINAKYSDLYDPCKFMILVNEEPLRGGTG
                     PDGIRVFAIVPSANDNYRPNAVMEFVGKDSASDAVMMWRIDYTKQGESSTQINWKRDK
                     ISSKRMLDFFKQTERLGTVWKDVDPNGFAIKTTTNNWILALAGYHGITPHTTPETLSD
                     PSFTPILTIGQLNPEKLSDGEYVIRGEENWNFINKIRGNDGFSDLVTMLTGGNVNTPS
                     KSYSVQPFVPREKFHQMTLSLTGGQLAIDIPTEILADRIMLLGAPGGDGPFADVLSET
                     YELRIDLLKKVNLLGDKVTMSLSSGGSWQTQIPLKEGINSGCAVFFNPRASFLLEHYA
                     EWVSKNPNHSFDQYLAALKNSMSSELDTAKARLSEYWFVWNPTEKLQKAREDKDRTIL
                     IPAYETLSNWYELALSTGYDPVGPNKFRNVKKLTGKMVDWFFNSETGLLSDASGTISN
                     GRIFINYSSLANATNATYREALMDGRDGGLGLRKLLNVLDGKAPTGSLILNLIDPENL
                     DSAKSGRVADLFVHTMKLLGNGSETNMPFYDIFREARQLTISASLGTLGSASASLDLP
                     GNKYDRLKNAENFKTSFQTGVKNFGGLPLDVLTPDSTIHITQFAGGGDPKFSVILSAD
                     DLSQRSLSSQQVGAVLEQYTKAGGNTGGRIEISMGEGTTKFWSLGTLNRTWAGLSSGV
                     NWCAARVVGFFSDKWGGYWEAGRKEIAAEWERSRYDNYGRGDGLDFVYYYSQTANGFE
                     LVGGVHRPVATEDQYQAFTVIQTGGVYRVVEAENSGAPRLLSEKYVEVRGWGYDPNGK
                     LTVSGHYNRGFFARCAGNTFIHSEFQTDAQNYSNAIMVFNQQDMAVASKLRSVAHVAT
                     SIKYAIQSVVANAWSWWGEQHVAVEIMGKGLIVGGAVAAAIFGGWLVFLKAGAVALAG
                     HGLMALGIKATGWGLIYVGFSMAQGIYTAGKYQEATYQGMSWQMAAQAAGGTDMVTKV
                     LALGQEMALNLAMLN"
BASE COUNT         1716 a         1975 c         1628 g         1819 t
ORIGIN      
        1 cgttcaacat tgccaggttc aaagccatct cctgcccgag cgcaagcact ttggtgacca
       61 tatccgttcc cccggccgct tgcgccgcca tctgccatga catgccctga taagtcgctt
      121 cctgatattt cccggcagtg taaataccct gagccatcga aaatccaaca tagatcaggc
      181 cccacccggt agctttgatg cccaaggcca tcaggccgtg gcctgctaat gcgaccgcac
      241 cggccttcag aaagacaagc caaccgccaa aaatcgccgc ggccacagca ccaccaacaa
      301 taagaccttt gcccatgatc tcgacagcaa catgttgttc tccccaccaa ctccaggcat
      361 tggctaccac actctgaatc gcgtatttga tgctggtcgc aacatgggcc acactgcgaa
      421 gcttactcgc gacggccata tcctgctggt tgaaaaccat aatcgcgttg gagtagtttt
      481 gcgcatcggt ctggaactca gaatggataa aggtatttcc cgcacaacgc gcaaagaacc
      541 cgcgattata gtgcccggaa actgtcaatt ttccattcgg atcgtagccc cacccgcgga
      601 cttcaacata tttttcgctt aaaagccgtg gggcaccaga gttttcagct tcgacaacac
      661 gataaacgcc gcccgtttga atgactgtga aggcctgata ctggtcctcg gtagcaaccg
      721 gccggtgaac accacctacc aattcaaatc cgttcgccgt ttgggagtag tagtaaacaa
      781 aatcgagccc gtcgcctcgg ccgtagttgt cgtaacgact gcgttcccat tcggccgcaa
      841 tctctttccg gcccgcttcc caatagccgc cccatttatc ggagaaaaat ccaacaactc
      901 gagcagcgca ccagttcact ccactgctca agccggccca cgttctgtta agagttccca
      961 agctccaaaa tttcgtggtc ccctcgccca ttgaaatttc gatcctgccc cccgtattcc
     1021 cgccggcctt agtgtactgc tcaagcaccg cacctacctg ctgcgaactt agggatcgtt
     1081 gggagagatc atcagctgaa agaattactg aaaatttggg atctccgcca ccggcaaact
     1141 gcgtgatgtg gatcgttgag tccggagtca gcacatccaa cggaagtccg ccgaaatttt
     1201 tgacacccgt ctgaaacgaa gtcttgaaat tttccgcatt tttcaaccga tcatacttgt
     1261 tccccggtag gtctaaagaa gcgcttgcgc tccccaacgt gcccaagctt gccgaaatgg
     1321 tcaactgcct ggcctctcga aaaatgtcgt aaaaaggcat gttcgtctcc gaaccatttc
     1381 cgaggagttt catggtgtgg acaaagagat cggcaacacg tccgcttttc gccgaatcca
     1441 aattttccgg atcaataaga ttgagtatca aacttccggt tggcgccttc ccgtcaagca
     1501 cattaagcaa ctttctgaga ccaaggcctc cgtccctccc atccatcagg gcctctcgat
     1561 atgtggcgtt tgtcgcattc gccaatgaag aataattgat aaagatccgc ccatttgaga
     1621 ttgtgcctga tgcatcgctt aataaaccgg tctccgagtt aaaaaaccaa tcgaccattt
     1681 ttccagttaa tttttttaca ttgcgaaatt tgttaggacc tacagggtca tacccggtag
     1741 acaaagccaa ttcgtaccaa ttcgatagag tttcgtaggc aggaataaga atcgtacgat
     1801 ccttatcttc tcttgctttt tgcaatttct ctgtcggatt ccatacaaac caatactctg
     1861 acaaacgggc tttcgccgta tcaagctcag aagacatcga attttttaag gccgccaaat
     1921 attgatcaaa ggaatggttc gggtttttgc taacccactc cgcatagtgc tccaataaaa
     1981 agcttgcccg gggattgaaa aaaacggcgc atcccgagtt aatgccctct ttaagaggaa
     2041 tttgcgtttg ccaggaaccg ccggaagaaa gcgacatcgt gactttgtca ccgagcagat
     2101 tgaccttctt caacaggtca attcgcaatt catacgtctc ggagaggaca tccgcaaatg
     2161 ggccatcccc gccgggcgct cccagcagca ttatcctgtc agccaaaatt tccgtcggaa
     2221 tgtcaatcgc aagttgccct ccggttaacg aaagggtcat ttgatgaaac ttttcccgag
     2281 gaacaaaagg ttggaccgag taggatttgg aaggagtatt tacatttcca cccgttaaca
     2341 tcgtcacaag atcagaaaat ccgtcgttcc cgcggatttt attgatgaaa ttccaatttt
     2401 cttcgccccg aatgacatat tccccatcgc taagtttttc tggattaagt tgcccaatag
     2461 tcagaatggg cgtaaacgat ggatcggaga gggtctcggg ggtcgtatga ggcgtgattc
     2521 catggtaacc cgcaagtgca aggatccagt tattagttgt tgttttaata gcaaaaccgt
     2581 tgggatcgac gtctttccat acagtaccca gacgttcagt ttgtttgaag aaatcaagca
     2641 ttctcttaga tgaaattttg tcccgtttcc agtttatttg ggtagagctt tccccctgtt
     2701 tcgtgtaatc aatgcgccac atcataactg cgtctgacgc gctatctttt ccgacaaatt
     2761 ccataaccgc atttggtcga taattatcgt tcgctgaagg cactattgca aacacgcgaa
     2821 taccgtcagg acccgttcca ccccgaaggg gctcctcatt caccagaatc atgaatttgc
     2881 agggatcgta caaatcggaa tactttgcgt taatcgcgtc tgtcaagccc tggtacatgc
     2941 cgagggaaaa attgtcaatt tcatcagcaa tcatttcgaa atttgtgaaa ccaaagcgcc
     3001 tgaccttgac atttttatct tgagaattcg gcagaattga tttgcgagcg aaagcagtta
     3061 aagtattttg ggtcacagaa tcaaactctc gctgatctcc tattggagta gcggccttgt
     3121 gcgtttcaat taagttataa gtcttatcac cgcccctctc gctcacaaaa accttatcac
     3181 ccattccgcc ttcgggtcgg ccgaaaaaga ttctattgcc agctatatca aaggattctg
     3241 tataaagaaa tcctgaaaat atccgctgtc cgtttttaac caggctaaag gtggtctccc
     3301 cgaacaactt ggcaagttca tcaaaaaccc cgggggacga tttgtagact gcctcgaacg
     3361 cggaaacgtc cgccgacaga aaccagtcga ttcggcatgg aggattggga ttcccggcac
     3421 ttgcctggat aaagtgatcc ccaccaaacg agaagccctt taattgctca ccattggggc
     3481 caacaccccc cggtaattcg ttttgattta tcgtggacgg aggtaattca cgaaccgcgt
     3541 caaaagtttc cccctggttc atggaaacgc tgaaaaacct ctgagcagga tcgtcgactt
     3601 tggtcaacaa cgcaaagatt gctgagcgcc ccttatgcag atggccgctc gcatacagtt
     3661 tgccttcaac ccagacagta actaagccat cccgctttac gactaattgg ccagggagtt
     3721 tgggttgatc tggcttctgg ttctgcagtt ctatgtccac attcattggt tttagagcag
     3781 atttgtcaaa agacacgcgg ctctgtccgg cataatcgcc cattccggtc agcggcgtat
     3841 cgtgagtgaa ccatccgtta acatccatca gtccctgcgc gcgccaggtt gcgttgaagt
     3901 tcgcgtcggt ccagacgacg taatcggaga caccgaattt cccgaattcc gccatcagcg
     3961 cagatctcag tggatcgtta tctgccaaaa caccatccgc gtggatcaca accttgtttt
     4021 ggaacccgcg cgtggttttc gggccaacct tgccgtcaaa tcgcgcggcg gtcgaaatct
     4081 gcgaagggtt taatgccgaa ctgacttcct gccacttgcc agccgccgtt tggggacttt
     4141 taccgacatt acctcgaaaa gcataaagtc cttcattcgc gccgcggcca atcaggaact
     4201 gcgtcacatt ttgcgccgcg ttaaatttcc ggtcagcctg ccagctgagc caggctccgc
     4261 cggaaacatt cgcgtcattt gtataaaatg atgtgagttc ccgctggccg aacgtcctgc
     4321 aaccttcgag gaacagctcg cgggtcgtgg gagcgatatc tccggccatc tgttctgttc
     4381 cgtttacata ttttttgacg cctgtgatat cgataccctg gaagaaacca aaattgatcg
     4441 tactgccgtt tgccgccgta cgcgtgtcat tcgcggctcg aatctcatta tccttaagct
     4501 tttcaaaagc ccccatggtg gccgtgaatt cccaggcagt tcgcgatacg gcacgcgtag
     4561 cagtatcaag tttcccgttc tggatcatgt agattttctt cgcctcgccc tttgctgagt
     4621 cggccgcata ggcgccaagc ccgaggaacg cgaattttgt atccggcgca tccatcttcg
     4681 tattcgcccg cccgaggaat tttagcagtt ccgtttcaac cctactttcc ccctcccctg
     4741 caacattcaa aaccgtggtt gtgtaccggg gaatgagaag atcggagaga atggcttccg
     4801 tgacctgcgt tgaggccgcg cttccgccca ctatgaccgt cgcttcgatc tctcgcgcaa
     4861 aattagcatc gcttcccaga aacccggacg atcgaatggc gcccagcatg gaactcgcat
     4921 caaaagtcgt gccccttgat tgatctctga caactccgtt atgctcgact ttaaaatcct
     4981 tcaccatcca gctctctaca ggggatgtag gtgcagattt atattgctcc catgtgggat
     5041 acagccaaaa cccagaggag aacgtcagag cagacggcgg gacggcgccg aacctgacac
     5101 cgtctgacgg cgcgacgaga actttgcctt cagtgcgagt cacttgaccg gtcgcgggat
     5161 cctggatttg attaatgaga taaagcgttt tttcaagttt gcctttcgcg agtcccgccg
     5221 tatctccctg gatctgatat tgtttcggtt tgtccgtttc tccatagaca agggtgaatg
     5281 aagaaagctg gcccatgaga tcgcggaact gtttcccttg agtaaaattt ccctctagga
     5341 atttctccat ggctccatcc ggcgaaccat tgaagaaaag cccataaccg ccctgcaaaa
     5401 ctttggcatt tttgaatgcc ttgtcaaaag ctcccgcgcc ctggacttcg tcatatttct
     5461 ctcttatcac attcagggcc ttttgatttt cccaggtctt ccggtcggtc gggatcatgt
     5521 aaacttcatt tcgctgttta gggtcaggtt gcgttgcaag aagtccgccg cgattttcgc
     5581 tccccacgcc tctccaaaga taatcccctg ccatcccccg ctcatccaga gtcgtttccc
     5641 aggcattcac tttgtcgcca gccctaaatc cttcggggat cttatcgttt tcgttatata
     5701 ccctgtccca aacacgtaag acattggttt gcggtaaatt gacttcatac tggaaacttc
     5761 tgataaatgc cacagcatcg gcctcggtca aaccttcgga aaggttactt gtcgcccaca
     5821 cgaccgtccc tgtttcaccg gctgtgcaag cgcctaaccc gtttcctttc aatgcctccg
     5881 cgggtagact ggccttaaca gccgcgagct cctctgaatt aagattctta agaccttcaa
     5941 caccaccgaa aatatcaaag gttttaccgg gcgcgatttg gcccttaaag tggcgaacag
     6001 cgtccgttga aaaatgctta ctaagattgc cgcttccatc atattgcgca aaaaacaagt
     6061 atctctgtgt tttgctgtcc gcaatccaga tattcatgtt ggcgatcccg ccgaggcttg
     6121 atgcactaat cgtcgtcgca tccgtgttcg tattcatctc acgaacgata ttctgccccg
     6181 cccgaagtac cgcgtttgcc gaaacgtccg aggctttatc gcctgagtca acatggtcag
     6241 cgcccgcgat ccgcgtgccg cgggcagagt aaaattcata actccagtgc tcagaaagcg
     6301 aagctgcctt atcggtcgga acagcatctt tttggggagt aaactgggct gtgaaactcc
     6361 aggtgccatc cgcatcagag aatgtcacga aacccgcaac agggaaggtt aaattgtcaa
     6421 acccacgagc ggtcaaggca ttgttttctg cccgcgtaat cgagacgggc ttgagcgaat
     6481 cttcttggat cgcggcaacg ctattcgttc cgacggaggc gtggaactgg gtgattgtca
     6541 ggacggtatt cgctgtttca tcgtacgttg tgctggctaa tttccatttt ccgtccgaag
     6601 aagtttgcgt tgccttcgtg gcatctactt tacccggtat aaattcagca ctaaatactg
     6661 tgaggccatt tgctccaagg agaggatcaa aaacaccaaa tcccgtgatc ggaagcgcct
     6721 gggaatcaag agcgtctccc gaataaatcc atttccccgt tggcttactc gtcgcactat
     6781 caatttctct gataagacct ttgttgataa ttcccaaagt tccaactact ttaggctcag
     6841 tgtttggtaa cgtgcttcca gactttgtgt ccgcccgggc aaaaacataa acattctgat
     6901 cttggttgtg gcttgagagc atgcgaacct tgccgccggc gtcaccaagt tcgaagacgc
     6961 ggcgcttcca gtcttcgggg cttttgaggc ttagtccctc ggccaataac ccgtgcggca
     7021 cgagagacca acccgcgaaa ttctgcgtca tggccaacgc cgtggactgg gagagattgc
     7081 taaagagcca ggtttcgcgg ccacccgtaa gaacctgttt tgcttcgccg aacgcagc
//
LOCUS       DAOSIO010000070         7130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_9566_length_7130_cov_4.190813, whole genome shotgun sequence.
ACCESSION   DAOSIO010000070 DAOSIO010000000
VERSION     DAOSIO010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9566_length_7130_cov_4.190813"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..761)
                     /locus_tag="PK590_03795"
     CDS             complement(6..761)
                     /locus_tag="PK590_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOG23750.1"
                     /translation="MPVSRSFGGSVPQTGVLKKWVVTPKMSGWCVGFPKGVFFCGVVL
                     GVLFCLTGCKTTDTQCQRISRQQALTIEGLNSEILRLNQEIDTTIGSRASLEKVQAPL
                     ASELSREIGTGVLTVSLERKGLVIRILESALFEPDQIDISDSATSLLDPIATYLSGPL
                     RENQVIIEGHLDGPLPDGSGWRSGWEYTQAIAVEVVHYFVDVAGLNSNRFVVAAFNDH
                     CRLNLLDRAGNRARNRRVEIVIVPKGTNVCAVV"
     gene            complement(778..981)
                     /locus_tag="PK590_03800"
     CDS             complement(778..981)
                     /locus_tag="PK590_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23751.1"
                     /translation="MMGKMKIEIRKPQHMMPKEPMSRDQSVAVTAGDKPQGVPFFFNF
                     LLLVSYVLCGVAIWFAMRIFLER"
     gene            complement(1003..2577)
                     /locus_tag="PK590_03805"
     CDS             complement(1003..2577)
                     /locus_tag="PK590_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOG23752.1"
                     /translation="MTPSETRACSRRKKSGKVPKHSLTPLERFMIPNPQWQEGYEAFS
                     GLFGNPIGWLILLDEHERHRLIRLDRKQNCPYFFKSGENQRQCEAFITKFVDQLIHNE
                     EMIERLPLFYRCASAKPCLAFPVRYLGSLKGFLFFDCVKNPEREVKRIIDPFFSFLST
                     HVELAYKNFELNNFYETVHPRALALSTMHSVHRVINSSLRLRELIPRIGRLSAQILKA
                     QGCSIMLTDSSRRYLNPVFSFGSHPKFVHRQRVRIGRGIEGRIADTGEFCLQRRYIAV
                     PFIDDDVVGVLMLWDKKDKQPFTRTDLEILKSLSEQAVVAIKNAQLFEQTEELTLGSI
                     KTINELLERHFGEKRGHLEVVGQIAVEVGKILELSGQELVMIERAILLLDTGQLGISE
                     RTWEKKEKLSPKELQQVRSIPLRGASLLKSMSSLKPIIPIVMHRHERYDGRGYPQGLK
                     GEEIPIGARIVSVVDSYIAMVSKRPYKPQLSVKEALEEIQKHKGAQFDPKVVDAFLKT
                     MKEHSILEKVQRYIDE"
     gene            complement(2574..3080)
                     /locus_tag="PK590_03810"
     CDS             complement(2574..3080)
                     /locus_tag="PK590_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014788102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOG23753.1"
                     /translation="MDRLWAPWRKTYLRSTRVKSKGCLFCGLRRSKDDRKNLVVFRGK
                     RFLLILNRYPYNNGHLLIVPKRHLACISQLDQEERQDFFTMLDLAQSALQTALKPHGF
                     NIGMNVSDVAGAGIPEHLHWHIVPRWKGDVNFMPVIAGDKVISESLDSAFVVLRQAIQ
                     AQKGLKKL"
     gene            complement(3025..4785)
                     /locus_tag="PK590_03815"
     CDS             complement(3025..4785)
                     /locus_tag="PK590_03815"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG23754.1"
                     /translation="MANKFGSTCPFLSLKHKVLEMPFLTRETVQERLHKRIEDGVNGF
                     RQNIGLIGSPGVGKSHLLKNFFQSISRDSRLLSIFVRGETIDDKQLVEQWLGAVLCSV
                     LIRKTSDIPSSLSGLIQHTEPLIPSTVQAVKHVQRLFHQEKKSSAVRELFGLSSMLAR
                     ETGKKVVLIIDELQGLEKLPVTDPFAMLGKQIMPAKEVLYLVTSSARDRALEIFRNKL
                     SLLFGNFELLELGPFGFFEMEKYLAGRMPTHRWTEPLKRFLFHMTDGEPLYVDLMIQC
                     IEKMGVREIPQPVTPASLIDAFCQELFDDRGRIALRFEQKIQQCASLSGKNGVHKRVL
                     ISLSQGRRKVIPIAASIAETVTETQKVLKRLVQEGLIVKSGSFYHLPDILFRFWIREV
                     YLKRHALFLPEGQFLRKSLFDKLNATCDSCARMHNEDVGARVETMLKEFRNDTVEFSQ
                     KKFQCPQFSETALRVLPNSLFAILGKGGRGRWLFYVGAETIDERAVENLLKDSKNLRN
                     IRRKILIALSGIEQNAKLIAQEAKMQIWDLRQLNVFMDYYDLPKIIITSQSRTHGTEQ
                     KTDGSIMGTVAQNVSALNSR"
     gene            complement(5093..5587)
                     /locus_tag="PK590_03820"
     CDS             complement(5093..5587)
                     /locus_tag="PK590_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOG23755.1"
                     /translation="MWQITLKENDLVAGLKASDFKDALTKLVGMIPDWRLGPKMKKDV
                     FQLLVDRERIGTTAIGGGVALPHCLSEDVREPIVMFGVSPDGLACPSLDGYPVHFIFM
                     LLLPKDEQAELRKRSVLQSAKWMLSDRSLRDELLDARSSADIYDCLAGRVGLPPRESV
                     VFEH"
     gene            complement(5559..5909)
                     /locus_tag="PK590_03825"
     CDS             complement(5559..5909)
                     /locus_tag="PK590_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23756.1"
                     /translation="MEISNQLLKPYIEKMETEWQQLYREVCGHSSDGVAAMKPTIEQR
                     MRQLEKFRTSAMYFDRDEKGVKQFPNLREALRLLKLDLRTTRRQWRRHLVSKIRTQKR
                     QERALYVANHPEGK"
     gene            complement(6089..>7130)
                     /locus_tag="PK590_03830"
     CDS             complement(6089..>7130)
                     /locus_tag="PK590_03830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG23757.1"
                     /translation="TGNSFQTIHFQKRPIRLCYQLARIEGRPFFVIQVAKPLDEIEAT
                     LNRLTIIIALIIPLGVMLAGWAGWFLAKRFLKPVDRMIHQSRTITAAYLKGRLPRTFS
                     LDELDRLAETLNEMIDRLEASTQAVREFSANVSHEFKTPLAIIRGEIDLALRRDRSQA
                     DLHKALRIISEETDGLTRLVNDLNFLVKSDAKQLTLQRRTLILNQLIDSVVSLYRERC
                     QKEGITLKYDQSDEVKIQGDDHHLKRLFINLMDNAVKFTDVGGSITISCKIRDGAGVV
                     RIEDTGIGIDPETLSHLGTRFYRADQARAKEGAGLGLSIVHAICDVHQAELQIESQVG
                     KGTAVSVKFHLA"
BASE COUNT         1965 a         1878 c         1506 g         1781 t
ORIGIN      
        1 gcgtctcata caaccgcgca aacgttcgta cctttgggga caatcacaat ttcaacgcgt
       61 ctgttgcgtg ccctgttccc agccctatca agcaaattca accgacaatg gtcattaaac
      121 gcggcgacca caaaacgatt agaattcaga ccggccacat ccacaaagta atgaaccact
      181 tcgaccgcaa ttgcctgggt atattcccac ccggatcgcc acccgcttcc atcgggaaga
      241 ggtccatcga gatgcccctc gataatgacc tgattttctc gtagaggacc ggacaaataa
      301 gtggcgatcg gatcaagaag cgaggttgcc gaatctgaaa tgtcaatttg atccggctca
      361 aacagcgcgc tctcaaggat ccgtatcaca agcccttttc gttcaagaga aaccgtgagg
      421 acgccagttc caatttccct cgataactcg gaggctaacg gggcttgaac tttttccaat
      481 gacgcccggg aaccaatcgt tgtatcaatt tcctgattga gtcggagaat ctcggaattc
      541 aaaccttcaa tggtgagcgc ttgctgtcgg ctaattctct gacactgggt gtcagttgtc
      601 ttgcatccgg ttagacagaa aaggacgccc aaaacaacac cgcaaaaaaa gacccccttc
      661 gggaaaccca cacaccagcc tgacattttg ggcgtaacga cccatttctt aagaacacca
      721 gtttggggaa cacttccccc aaaactcctc gacaccggca taggaatcct tcaaacccta
      781 gcgctctaaa aaaattcgca tggcaaacca gatcgcgaca ccacaaagca catacgaaac
      841 caaaagaaga aagttaaaga aaaaaggaac cccctggggc ttatcgcccg ccgtcacagc
      901 gaccgattgg tcccgcgaca ttggttcttt cggcatcatg tgctgaggtt ttcttatctc
      961 aattttcatc ttccccatca ttctttctcc ttcccgtaac cttcattcat cgatataccg
     1021 ctgaactttt tccagaatcg agtgctcttt catggtttta agaaacgcgt ctacaacttt
     1081 gggatcgaac tgggccccct tgtgtttttg aatctcttcc agagcttctt taacggaaag
     1141 ttgcggcttg tagggacgct tcgagaccat ggcaatgtag gaatcgacga ccgaaacaat
     1201 ccgggcgccg atcggaattt cctcaccctt taaaccttgt ggataaccgc gcccgtcgta
     1261 acgttcgtgg cgatgcatca caatcggaat gatcggtttc aacgacgaca tggatttgag
     1321 caagctggct ccccggagag gaatacttcg cacttgctga agctctttag gagaaagttt
     1381 ttctttcttt tcccaggttc gctccgaaat ccccaactga ccggtatcca acagtaaaat
     1441 cgcccgttcg atcatcacaa gttcctggcc ggagagttcc agtatcttcc cgacctcaac
     1501 cgcgatctgg ccaaccactt ccagatgccc tcgcttctcg ccaaaatgtc tttccagaag
     1561 ttcattgatg gtcttaatac tgcccagcgt caattcttcc gtctgttcaa acaactgggc
     1621 atttttgatc gcaaccaccg cctgctccga gagcgattta agaatctcaa gatccgtgcg
     1681 cgtgaatggc tgtttatcct ttttatccca caacatcaga acacccacca catcatcgtc
     1741 aatgaacggc acggcgatat acctccgctg caaacaaaac tcccccgtat ccgcgatgcg
     1801 gccttcaatt cctcgtccga tccggacacg ctgacggtgc acaaatttag gatgacttcc
     1861 aaaagaaaaa acagggttca aatatcgccg cgacgaatcc gtcagcatga tggaacaacc
     1921 ctgagccttg aggatctgag cgctcaaacg gccaattctt gggatcaact cccgaaggcg
     1981 aagcgaggaa ttgatcacgc gatggacgga atgcatggtt gaaagagcca gagcccgggg
     2041 atgaaccgtt tcataaaaat tattcaattc aaaattcttg taagccaact ccacatgagt
     2101 tgacaaaaag ctaaaaaaag ggtcgataat tctcttaact tcccgctcgg gattcttgac
     2161 acaatcaaaa aaaagaaagc ccttcaggga tccaagataa cgtaccggaa acgcgagaca
     2221 gggctttgcg ctcgcgcatc ggtagaataa gggaagccgt tcaatcattt cttcattgtg
     2281 gataagctga tccacaaatt tcgtgataaa cgcttcgcac tgccgctgat tttcacccga
     2341 tttgaaaaaa taaggacaat tctgttttcg atcaagccgg attaggcggt gcctttcatg
     2401 ttcatcaaga agtatgagcc atccgatggg gtttccaaag agaccggaga atgcctcata
     2461 cccctcttgc cattgaggat tcgggatcat aaaccgttcc agcggggtca gggaatgctt
     2521 cggaactttt ccagactttt tccggcggga gcaagctcgg gtttcagacg gtgtcataac
     2581 tttttcaaac ccttctgtgc ttgaatcgcc tgccgaagca ctacaaaggc tgaatccagg
     2641 gattcggaaa taactttatc tccagcaata accggcatga aattcacgtc ccctttccaa
     2701 cggggcacaa tatgccaatg caagtgctcg gggatccccg cgcccgcgac atccgaaaca
     2761 ttcatcccga tattgaaacc gtgaggtttg agtgccgttt gcagagcgga ttgcgctaaa
     2821 tccagcatcg taaaaaaatc ctgcctttct tcctgatcaa gctgagaaat acacgccagg
     2881 tggcgcttgg ggacaattaa caaatggccg ttgttgtaag gatagcggtt caggattagc
     2941 aaaaatcgtt taccgcgaaa gacgacgagg ttcttgcggt cgtccttgct tcgccgtaat
     3001 ccgcaaaaaa ggcaaccttt cgacttaacg cgagttgagc gcagatacgt tttgcgccac
     3061 ggtgcccata atcgatccat ctgttttttg ttctgtgcca tgagtcctgc tctgggaggt
     3121 gatgataatc ttgggtaaat catagtaatc cataaaaaca ttgagttgcc ggagatccca
     3181 gatttgcatt ttggcctcct gagcgatcaa tttcgcgttc tgctcaatgc cactgagagc
     3241 gatcaaaatc tttcttctga tattccgaag atttttagaa tctttaagca aattctctac
     3301 cgcgcgttca tcaatcgttt ccgcgccaac ataaaaaagc catcgcccgc gcccaccttt
     3361 tccaagaatc gcgaaaagag aattcggcaa aactcttaac gcggtttcag aaaactgcgg
     3421 acactgaaat tttttctgcg aaaattcgac ggtatcgttc cggaactcct taagcatcgt
     3481 ttcgacgcgg gccccgacat cctcattgtg catccgcgcg caggaatcac aggtggcatt
     3541 taatttatca aaaagtgatt tccgaagaaa ctggccttcg ggaagaaaaa gcgcgtgccg
     3601 tttcagataa acctcacgta tccaaaaacg gaacaaaata tccggcaagt gataaaagga
     3661 gccactcttg acaatcagtc cttcctggac gagccgtttc aacacctttt gagtttcggt
     3721 gaccgtctcc gcaattgatg ccgcgatcgg aatgaccttg cgccgtccct ggctaagaga
     3781 aatcaggacc cgcttatgaa caccattttt cccggacaaa ctcgcgcatt gttgaatctt
     3841 ctgttcaaat cgcaacgcaa tccggccacg atcatcaaaa agttcctggc aaaaagcgtc
     3901 gatcagggag gccggggtca ccggttgcgg aatttcacgc acccccatct tttcaatgca
     3961 ctggatcata agatccacat aaagaggctc accgtctgtc atgtggaaga ggaaacgctt
     4021 gagcggttcc gtccaccggt gagtaggcat gcgacccgca agatattttt ccatttcaaa
     4081 gaaaccgaag ggacccagct caaggagttc aaaattacca aataagagag aaagcttatt
     4141 tcgaaaaatc tcgagcgcac gatcacgcgc tgaactcgtc acaagataca aaacttcctt
     4201 tgccggcata atttgttttc caagcatggc aaaagggtcc gtgaccggaa gtttttcgag
     4261 tccttgcaac tcatcaatga tcagcaccac ctttttgccc gtttcacgcg cgagcatcga
     4321 actcaaacca aaaagctcac gaaccgcgga agattttttt tcctgatgaa acaggcgctg
     4381 aacatgctta acagcctgta ccgtcgaagg gataaggggc tccgtatgtt ggattaaccc
     4441 cgaaaggctg gagggaatgt cagaggtttt gcgaatcaag acactgcaaa gaaccgcgcc
     4501 gagccactgt tccaccaatt gcttatcgtc gatcgtctcc cccctgacaa atatagacaa
     4561 gagtctcgaa tctcgtgaaa tcgattgaaa aaaattcttg agcaaatgag atttgccaac
     4621 tccgggggaa ccgatcagac cgatattttg tcgaaaacca ttcacaccgt cttcaatgcg
     4681 tttatgtaaa cgctcttgga cagtctcgcg cgtcagaaaa ggcatttcga gaaccttatg
     4741 cttgagcgaa agaaaagggc aggtagaacc aaacttgttc gccacgactg aaaattaact
     4801 tgtataaaac caacccttgc aacttctgcg ccttctcaac cgcttttttt taactcaaat
     4861 tttggcacgc ttacgtgctg atatactact tttttgaaat gagaaagcaa gtcctaaaac
     4921 acactttttg cgtgagtttt tttgtcgaac ttcactttca aacaaaaaca atcccacagc
     4981 ttcaaccgct acaaaaaaaa cctgatcgga tgcgttgtca gaccccccgc cttacaacat
     5041 catccgatca ggcttcaccg ctcaaacaaa aaacaccgtc ccaaaatccg tttcaatgtt
     5101 caaaaacaac tgactctcgc ggcggcaagc cgacgcgtcc cgccaagcaa tcataaatat
     5161 cggccgacga tcttgcatcc agaagttcat cccgcaacga tcgatcggaa agcatccatt
     5221 tagcactttg aagtacggat cgttttctca gttccgcctg ttcatccttg gggagtaaca
     5281 gcataaaaat aaaatggacg ggatatccgt caagagacgg acacgcaaga ccatcagggc
     5341 taacaccaaa catcactatc ggttcacgga catcttcgga caagcaatgt ggcagagcta
     5401 cccctccccc gattgctgta gttccgattc gttcgcgatc caccagtagc tgaaatacat
     5461 cttttttcat cttaggacca agtcgccaat cgggaatcat tccgacaagt ttcgtcaggg
     5521 catctttaaa atcggaagcc ttcaatcccg caaccagatc attttccttc agggtgattt
     5581 gccacataca gcgcacgctc ctgcctcttt tgtgtccgga tttttgacac caaatgccgc
     5641 cgccactgac gccgtgtcgt cctgagatca agcttcaaaa gcctcagggc ttcacgaaga
     5701 ttcgggaatt gcttaactcc tttttcatca cgatcaaaat acatcgcgga agttctaaat
     5761 ttttccaatt gccgcatccg ttgctcgatg gtcggtttca tcgcggcaac cccatcgctc
     5821 gaatgtccgc aaacttcccg gtaaagttgc tgccattctg tttccatttt ttcaatataa
     5881 ggctttaaaa gttggttact gatttccatt ttttcacctt tttaaatatc aattattatc
     5941 aattcaatat ttttaattac cgtttatttg cttccttaaa cataaaatag ccggtttaac
     6001 attaaaaaag gattaaaaat atgttaaaaa atttaaattc aatggttata ggacttaagg
     6061 cgggccttgc acttattaaa tagaggtctc aggcgagatg aaacttcaca gaaacagcag
     6121 ttccctttcc gacctggctt tcaatttgta attcggcttg atggacatca caaatcgcgt
     6181 gcacaatact cagcccaagc ccggcccctt cttttgccct agcctggtca gcacgataaa
     6241 atctcgtccc caaatgactc aaggtctctg ggtcaatccc gatgcctgtg tcctcaatgc
     6301 ggacaactcc ggcaccgtca cggattttgc aactaatcgt aatcgaacct ccgacgtcgg
     6361 taaattttac ggcattgtcc atcaaattaa tgaaaagacg ttttaagtga tggtcgtccc
     6421 cttgaatttt aacttcatcg gattgatcgt atttcaaagt aatgccttcc ttctgacacc
     6481 tctcgcgata caagctgact accgagtcga tcagttggtt aagaataagc gttcgacgct
     6541 gaagcgtcag ctgtttggca tcacttttga caagaaaatt cagatcattc accagacggg
     6601 tcaggccatc ggtttcttcg ctgataatcc ttagcgcttt gtgtaaatca gcctgtgatc
     6661 ggtcacggcg taaagccaga tcgatctctc cgcgaataat cgccaacggc gtcttgaact
     6721 cgtgggaaac attggcgcta aactcccgga ccgcttgggt ggaagcctcg agacgatcaa
     6781 tcatttcatt caacgtttcc gccaaacggt ccagttcatc aagagaaaaa gttcgcggca
     6841 agcgcccttt taaataagcc gccgtgatgg ttcgcgattg atggatcatc cgatcgaccg
     6901 gcttcaaaaa ccgttttgcc aaaaaccatc cggcccaacc cgcgagcata acccccaggg
     6961 gaataataag cgcgatgata atcgtcaatc ggttcagagt tgcttcaatt tcgtccagag
     7021 gctttgccac ctgtatcaca aagaaaggac gtccttcgat ccgcgccaac tgataacaaa
     7081 gccggatggg ccgtttctga aaatgaatgg tctgaaatga atttccagtt
//
LOCUS       DAOSIO010000071         7092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_9637_length_7092_cov_3.979821, whole genome shotgun sequence.
ACCESSION   DAOSIO010000071 DAOSIO010000000
VERSION     DAOSIO010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9637_length_7092_cov_3.979821"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1585)
                     /locus_tag="PK590_03835"
     CDS             complement(<1..1585)
                     /locus_tag="PK590_03835"
                     /inference="COORDINATES: protein motif:HMM:NF019872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HOG23758.1"
                     /translation="MRSYAYGFPRLGEKREFKYSIEKFWRHECEEEELLRVLSSIQQR
                     NLREYGQYIDDYPRGEMTAYDPMLDIAVMCGFCNVCDLKEYYEYCRGADAFEMTKWFN
                     TNYHYLVPDFTYWKKPDFKENCENKMLCFKKCDFPQWIGPFTFLKLSKGLAGARFAKF
                     FMALIEIYGRVLSRYAKAQLDEPAFVLDLTVEEVHLIKQGYQRFRDCGCSITLMTYYE
                     SVDFMEDFLSLPVAAVGLDFVRGDNYERLAKLKFPGDKTLIAGLVDGRNVWRNSIGCS
                     LEKLKNLSQKTANLMVSNAAPLYHLPITLSAETRLSPELKGQVAFAREKLEEIQWIAD
                     AFSGKQIPGASLKTGAFGVNPNVQERVKSLKASDFVKGVPYDERRAIHQKRLKLPLFP
                     TTTIGSFPQTAEVRQKRAAFKRKEISRIDYRHFLQREIDRLISFQEDLGLDVFVHGEF
                     ERTDMVEFFAKKLLGFETTLEGWIISYGTRTYRPPLLFGDVSRPEPMTLLEILHAQSR
                     TRKPVKGMLTGAVTIIAWSY"
     gene            complement(1598..3403)
                     /locus_tag="PK590_03840"
     CDS             complement(1598..3403)
                     /locus_tag="PK590_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23759.1"
                     /translation="MKSSPHSFHIPVMGTGYSIDTPLKVARFGITSTISLVDHFLIGK
                     IRKFYSQQFGEEFFEIPKGNPDARALEIKEYLNLVKRIVKSQIDEIRGEDFEKGGSLD
                     QYFEMLPEGSPLKERYQRMLAIQIPEEKSRIQLFLKRQIIPGAIDVNIMTKLDRASYR
                     NGSAQGHEFSDALSALRGFAESDLCSAMVFSARINLSLFSYLENFDDFYVNQEGESHK
                     HIILKVSDYRSAFIQGKLFAKKGLWVSEFRIESGLNCGGHVFPTQGVLLGPVLDEFKS
                     RKREFVDSLFDVFQESLWQKKGVLVEEPPPLRLTAQGGICSAGDDLLLRKYFQLEGTG
                     WATPFLVVPEVTAVDSQTLRLLLNASKDDLYLSDASPLGIPFRNLRNSPRQQQMMQQQ
                     ARGELGAVCRGKFLALDTKFCKEGLCPASRKYQLSLKDSGEKCSCSKRNPSFMEKECI
                     CTGLGDSALLKYGIPAGERPLAASICPAPSIIYFKRIFSLREMVDHIYGRRNLLDGRE
                     KFSNLFITELRLYIDFLKEKVINVIQKMDKKSFAYFSEFKKTLLLGIEYYRKEVQRWS
                     SELLDKETFLSELQLLENELRGFDLLPCPEVLKIA"
     regulatory      complement(3560..3776)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(3846..4598)
                     /locus_tag="PK590_03845"
     CDS             complement(3846..4598)
                     /locus_tag="PK590_03845"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HOG23760.1"
                     /translation="MKTIRDYFSFFYLWGLIAVSSVFLFLVALVIWFMTVCFDRRLAW
                     LHFFTCFWGALYTWLNPFCSVEICGKEQINPKQTYIMIANHTSMMDIFSIFRLFTYFK
                     WVAKESLFTIPFVGWNMFLNGYIKINRRGKSHKKMFGACFHHLLEHRSLFIFPEGTRS
                     KDGYLGNFKTGAFRLALKAKTDILPITIHDSHGAFSSKAALRKRRRKIKVAVHRPLRY
                     EQFKMWSVEVLTKKIQDEIKGFLLVGDSAFIG"
     gene            5318..5737
                     /locus_tag="PK590_03850"
     CDS             5318..5737
                     /locus_tag="PK590_03850"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOG23761.1"
                     /translation="MGPLTDKRDSRGFTLIEVMVTLFVAAIAIAGYVGSNIATQRQAA
                     ELHERAIAVQEAQRVIEQIRDKARTNNFPENVLAAFPNGGTVAGMNALPNEQVRVDYV
                     NPAATPLDTTVTVTWRSHASRQQTAALRAYITKRKMP"
     gene            5734..6282
                     /locus_tag="PK590_03855"
     CDS             5734..6282
                     /locus_tag="PK590_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23762.1"
                     /translation="MKKLASQKGFNLIEAMLAVALSSVVVFGVFLLFRVGSEQSQINQ
                     SLMNLKDSSREAIYKMVQEIRQSAPGHVIIEANGQSIRFKIPHPDHLIGPDFDVDWDS
                     AHTITYTLGGTNSRQIIRTDTTTGMTSIIANDITALRFSENSSPSRIVTITLDTQKTL
                     SSGRAVPSAPLQITAQADVRNP"
     gene            6279..>7092
                     /locus_tag="PK590_03860"
     CDS             6279..>7092
                     /locus_tag="PK590_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23763.1"
                     /translation="MKTHPFSQIGNQRGFVLTAVYLILALATVFSVAYFSRCVAFVQA
                     NERNIHKTVAFNMAESGIDWAINQLTSNAEYTGTSAYVPLDTNYMKGGFTVSVTKDDS
                     DPKIRIIQANGFAPSNDPTQRGYQTSSLLAYVRSGSSSAFRTGIFATDSIQMSGNART
                     DSYDSRNGAYGGSNVAYNGSIETNTTGSKKVTLSGNVMIQGSVTVGPTESPSSVVSTS
                     HNAVITGGISSNPAPVIFSDAVAPVSADGELKLSGNTTYYLMPGTYHFTSMSI"
BASE COUNT         2024 a         1784 c         1420 g         1864 t
ORIGIN      
        1 aataactcca agcgataata gtaaccgcac cggtaagcat tcctttcacg ggtttccggg
       61 tcctgctctg cgcatgaaga atctccaaaa gagtcatggg ttcaggtctt gaaacatctc
      121 cgaaaagtaa cggcggacga taggttcgtg ttccatacga aataatccag ccttcaaggg
      181 tcgtttcgaa tcccagaagt tttttagcaa agaactcgac catgtcggtt ctttcaaatt
      241 ctccgtgcac aaaaacatcc agtcctaaat cttcctgaaa ggatataagt cgatctatct
      301 cccgttgaag aaaatgccgg taatcgatcc gggagatttc tttccgtttg aacgccgcgc
      361 gcttttggcg gacttccgct gtttgcggga aactaccaat ggtcgtcgta ggaaacagag
      421 gtagcttcag tcgcttttga tgaatcgcgc ggcgttcatc ataagggaca cctttgacaa
      481 aatcggatgc cttcaaagac ttgacccgct cttggacgtt agggttgacc ccaaaagcac
      541 ctgttttcag ggaagctccg ggaatttgct tcccggagaa cgcatcggca atccattgta
      601 tttcttctaa tttttcccgg gcgaaagcaa cctgaccttt caattccgga gagagtcgag
      661 tttccgctga aagggtaatt ggaaggtgat aaagcggagc agcgtttgaa accatcaaat
      721 tcgcggtttt ttgggaaagg ttcttcaatt tttccaaaga gcacccgata gagttccgcc
      781 atacatttct gccatcgacc aatccggcaa taagtgtctt gtctcctgga aactttaatt
      841 ttgccagacg ttcataatta tcaccccgca caaaatccag ccctacggca gcaacgggaa
      901 gagacaggaa atcttccata aaatcgacag actcatagta ggtcatgaga gtaattgagc
      961 aaccgcaatc tcgaaatcgc tggtatcctt gcttgatcag gtgaacttct tccaccgtca
     1021 gatccagcac aaaagctggt tcatccagtt gcgccttcgc gtaacgcgat aaaactcttc
     1081 cgtagatttc tatcaatgcc ataaaaaatt tcgcgaatct cgcgcccgca agccctttgg
     1141 acagtttgag aaatgtaaag ggccctatcc attgaggaaa atcacacttc ttgaaacaga
     1201 gcatcttgtt ttcacaattt tccttgaaat caggtttttt ccaataagta aaatccggca
     1261 ccaggtaatg gtaattagta ttgaaccatt tggtcatttc gaaagcatcc gcaccacggc
     1321 aatactcata atattctttc aaatcacaga cattacaaaa cccgcacatt accgcgatat
     1381 ccaacatcgg atcatacgcc gtcatttctc cccgcggata atcatcgata tattgaccat
     1441 attccctgag attccgttgc tgaatagagg ataaaacacg caacaattct tcctcctcac
     1501 attcatggcg ccaaaatttc tcaatgctgt acttgaattc tctcttttcc cctaatctcg
     1561 ggaaaccgta agcataactc ctcatgtttt tcctccttca ggcgattttt aaaacttcgg
     1621 ggcaaggcaa gagatcgaag ccccgcaatt cgttttctaa aagttgcaat tcacttaaaa
     1681 aagtttcctt gtccaaaagc tctgaagacc agcgttgcac ttctttcctg taatactcga
     1741 tcccgagcaa cagggttttt ttaaactcag aaaaatacgc gaatgatttt ttatccattt
     1801 tctgaatgac gtttattact ttttctttta aaaaatcgat gtacaacctc agctccgtaa
     1861 taaacaagtt ggaaaacttt tcccggccat ctaaaagatt tctgcgccca taaatatgat
     1921 ccaccatttc tcgaagagaa aatatcctct tgaaatatat gatgctgggt gctgggcaaa
     1981 tcgacgccgc caggggcctc tccccggcag gaataccgta cttcagcaac gcactatctc
     2041 ccaagcctgt acagatacac tctttttcca tgaacgaagg atttctttta ctacaagagc
     2101 atttttcgcc actgtccttc aaagagagtt gatattttcg ggaagcggga caaagacctt
     2161 ccttgcaaaa ttttgtatcc agggccaaga acttgccgcg acagaccgct cccagttcac
     2221 ctcgagcctg ttgctgcatc atttgctgtt gtctcggact attcctcaaa ttacggaaag
     2281 gaattccaag gggagaagca tcgctgagat acaagtcgtc cttggaggca ttaagaagta
     2341 accgtaatgt ttggctgtcc accgccgtaa cttctggaac taccaaaaag ggagtcgccc
     2401 agccggtacc ttccaactga aaatacttcc gtaacagcaa atcatcccct gcagaacaga
     2461 ttccgccctg tgccgtaaga cgcagagggg gaggctcttc aaccagaact cctttctttt
     2521 gccacaaact ctcttggaat acatcaaaaa gggaatcaac aaattccctt tttcttgatt
     2581 tgaattcgtc aagcaccggc cccagcaaca ctccctgtgt cgggaaaaca tgtccaccgc
     2641 aattcaggcc ggattcgatc cggaattctg aaacccacaa tccttttttt gcaaaaagct
     2701 tcccctggat aaatgctgaa cgataatcgc ttactttaag gataatatgc ttatgggact
     2761 ctccttcctg attaacatag aagtcgtcga aattctcaag ataagagaaa agtgacagat
     2821 tgatcctggc tgaaaaaacc atagctgaac aaagatcgct ctcggcaaaa ccgcgtaacg
     2881 ctgacaaggc atcagagaat tcatgccctt gggcgctacc atttcgatac gacgctcgat
     2941 ccagttttgt cataatattc acatcaatag cgcctggaat gatctgtctt ttcagaaata
     3001 gttgaatccg acttttttct tcagggatct ggatcgctaa catccgttga taacgttctt
     3061 ttaatggaga accttccgga agcatttcaa aatactgatc gagagaacct cctttctcaa
     3121 aatcctcgcc tcgaatctca tctatttgag atttgactat tcgtttaacc agatttagat
     3181 actcttttat ttcaagagcc cgggcatcag gattgccctt gggaatttca aaaaattcct
     3241 caccgaattg ctgactgtaa aatttcctga ttttcccgat gagaaaatga tccacaagag
     3301 aaatcgtcga agtgattcca aaacgggcta ccttgagtgg cgtatcaatg ctgtagcccg
     3361 tccccatgac cgggatgtga aaagaatgtg ggctactctt catgggagaa ttccttcctg
     3421 gctctctggc aaaaccaact gttcttgtca gaaattccgg atcggatcgg ggggaaagac
     3481 ctgaaaagta aaaacttcaa aaacaaaaat ggggaaacgc tattttgatt cattttccgc
     3541 tcctccctcg agagcttaaa atttaagatt ggatctcagc aggtcttctg acttggggat
     3601 cttcctactc caaagcgtct tcccgaggat caaaaattcc ttacaacctc agtgactcaa
     3661 gctttgtttc gtccccctca cagcggcggg gccgttctgg tttctcacca gattcccttt
     3721 taagctccaa gtctccttca taagagacct ggaacactaa gatcaagcga tttaaatgta
     3781 aaaacttcag aaaacaggaa ccgtcttcac ccttttctca aaatctcctc ctttccgatt
     3841 catcttcatc caataaaagc actatcacca accaacaaaa atccctttat ttcatcttgg
     3901 attttctttg tcagaacctc cacactccac attttaaatt gttcataccg taacggtctg
     3961 tgaactgcga ctttaatttt cctccgcctc tttctcaacg cggctttact ggaaaacgcc
     4021 ccatgagaat catgtatcgt tattggtaaa atatccgtct ttgccttgag agcaagtctg
     4081 aaagctcctg tcttgaagtt tccaaggtag ccatccttgg acctcgttcc ctcggggaaa
     4141 ataaatagtg aacgatgttc cagcaagtga tggaagcacg ccccaaacat ttttttatga
     4201 cttttccctc gccgattaat tttaatataa ccatttaaaa acatattcca accaacaaaa
     4261 ggtattgtga aaagactttc cttggcgacc cacttaaaat aagtaaagag ccggaaaata
     4321 ctaaaaatat ccatcatcga tgtatgattg gcgatcataa tatatgtctg ttttggattg
     4381 atctgttcct tcccacagat ttcaaccgaa caaaaaggat tgagccacgt gtatagggcc
     4441 ccccaaaagc atgtaaaaaa atgcaaccaa gcaagtctgc gatcaaaaca aaccgtcata
     4501 aaccaaataa ccaatgccac caaaaataaa aaaacggaac taacggcaat aagcccccat
     4561 aggtaaaaga aagaaaagta atctcgaatt gttttcatag gaactatatg gcattacccg
     4621 gaaactcaaa acacactact ctggaaccag acacaaaaaa accctctccg cctccaagta
     4681 cgggcagaaa gggtctgtta gatgcgcctc tgctcggaga ctttcgagat ctcaaatgtg
     4741 aaacctggcg tacatttcat gacaagcctg tattctgact tactccggcc tttaaaccga
     4801 agcgacagta gcggcactgt ggagccattt cgccacgact aaagcgaaac tctcacttcc
     4861 caaacgcact tgtcatccag tttttaaaag aacgttgggt agtataggac gaaaaaattt
     4921 aaaacgcaag aattttatgg ggaactttta cgggggaagg gtgtgtccca ctatggaagt
     4981 ttaagaatat tcgtgataat cgtccatgca ccggttgcat tctgtgttat ggcttatttt
     5041 tctaaaaata aaatctataa gctttttcag gataatcgtt tttctcctta ccctttaccc
     5101 cttcaatagt tacaactttt cttggcaata aacacgaagt gacatctaac ttttttaaaa
     5161 actataaaat gattaaaaaa ataataataa ataaatctat gctatgtctt gcttcttttt
     5221 gcgtgttgca tataatctgg aagcccatta caattgtagc ggggttgaca taaaccagta
     5281 gtgtctcgac agagaaagag agagaggaga aaaacaaatg gggcctctta cggacaaacg
     5341 tgattcccga gggttcacgc tcatcgaagt gatggttact ttattcgtgg ccgcaatcgc
     5401 catcgcggga tatgtcggat ccaacatcgc gacccagcga caggccgcgg agttacatga
     5461 gagggccatc gcggtgcaag aggctcaacg cgtcattgag caaattcggg ataaagccag
     5521 aacaaacaat tttcccgaaa atgtcttggc cgctttcccc aatggcggaa ccgtcgccgg
     5581 aatgaacgct ctccctaacg aacaggttcg ggttgattac gtaaatccag cggctactcc
     5641 tctggacacc accgtcaccg taacctggcg atcccacgct tcccgacagc aaacggcggc
     5701 gctgagagct tatatcacaa aaaggaaaat gccatgaaga aactcgcgtc acaaaaagga
     5761 tttaatctta ttgaagcgat gctcgccgtg gcgctttcaa gcgttgtggt tttcggggtc
     5821 tttttgcttt tccgggtcgg aagcgagcaa tcccaaataa accagtcgct catgaatctc
     5881 aaagactcct ctcgcgaagc tatttataaa atggtccagg aaattcgcca atccgctccc
     5941 ggccacgtga tcattgaagc taacggtcag tcgattcgtt ttaaaattcc tcatccggat
     6001 cacttgatag gccccgattt tgacgtcgac tgggattccg cgcataccat tacctacact
     6061 ctcggcggga ctaactcccg gcaaatcata cgaaccgata ccacaaccgg gatgacttcc
     6121 atcatcgcta acgatatcac ggccctgcgg ttctctgaaa attcttcgcc atccaggatt
     6181 gtgacgatca ctttagatac ccagaaaaca ttatccagcg gacgagcggt tccatccgct
     6241 ccccttcaaa taacggctca ggcggatgtg aggaacccat gaaaactcac cccttctccc
     6301 aaatcggaaa tcagaggggg ttcgtattga ccgcggttta tctcatcctc gcgctcgcga
     6361 ctgttttttc agttgcttat ttctcccgct gtgtcgcatt tgtccaggct aatgagcgca
     6421 acattcacaa aactgtggcg tttaacatgg ccgaatcggg catcgattgg gccatcaacc
     6481 agttaacttc aaacgccgaa tacaccggaa cctccgccta tgtcccgctg gacactaatt
     6541 acatgaaggg cggattcacg gtctcggtca ccaaagacga ctctgatccc aaaattcgga
     6601 ttatccaggc aaacggcttc gcaccttcca atgaccctac ccagcgtgga tatcaaacat
     6661 ccagccttct tgcttacgtt cgatccggat ccagtagcgc ttttcgcacc ggaattttcg
     6721 cgacagattc cattcaaatg tcgggaaacg cccgaacgga ctcttacgac tcccggaacg
     6781 gggcttatgg cggaagcaat gtcgcctata acggcagcat tgagaccaat acaacgggct
     6841 ccaaaaaagt cacgctgagt ggaaacgtta tgatccaggg gagtgtcact gtcggcccga
     6901 cagaatcgcc ttcttcggtg gtgtcaacaa gccataatgc cgtaattacg ggaggcattt
     6961 catccaatcc ggctcccgtc attttctctg atgctgtggc tccggtaagc gcggacggtg
     7021 agctcaaact tagcgggaac acaacgtact atctcatgcc cggaacctat cactttacgt
     7081 ccatgagtat aa
//
LOCUS       DAOSIO010000072         6943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_9893_length_6943_cov_4.140825, whole genome shotgun sequence.
ACCESSION   DAOSIO010000072 DAOSIO010000000
VERSION     DAOSIO010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6943
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9893_length_6943_cov_4.140825"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PK590_03865"
     CDS             <1..228
                     /locus_tag="PK590_03865"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOG23764.1"
                     /translation="TDLAEKEKAELLILESYLPKQLSDEELRAEIKKAMQISGAQNPS
                     DIGKLMKTLMPLIQGKADGKRVQAAVKDLLK"
     gene            complement(420..6833)
                     /locus_tag="PK590_03870"
     CDS             complement(420..6833)
                     /locus_tag="PK590_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23765.1"
                     /translation="MNGTVTTEKEKRLAEIGKARDAAQSERDVAREKFGNDSNEAKIA
                     DEKVESIIAMKKETEEKTGSFEKMGPFVLISGELIATKDGCSIGNGAWLERVHWRGDI
                     QIQDGARVSDTVMSPVRVDRDERVMFVRRAEREGSLRVGKNSEVYFCASNDISVTENK
                     MLAYQGMRSGHFSRTGIARSPDQKNGIVIKQYGFLFERIKYAVMDVLALIMANAREVK
                     DQAALARQSVTSRQFWAKDRSVISNLGVAENRRNLATAIRTFIAETTAGTGLRQVFFA
                     TIGAGTVALAVAFPATLPFVGTALIFVFWQPMELLQNLRHPGKMFFKGPSSSLWVAGG
                     AVSSILLGGSFLYFGIAAGVIWICYLGARILGQGAVETGIFASAWKRESEFRKGIREE
                     IQSVLRDTTSGAARWQDIAGARAALLGNPNITSEVISLLEKPAPYEQDEIEADAVRQI
                     KKLVDERQELILKLQGAKQIVEKIKIRQMIEALESRLATDLADAILANHGASRIEKSN
                     RDRKGLIQVIKKNLHYGQLSIEAGTTAEIAYNPAVSGGFPEASRRLEESEAERKQRIA
                     ANERWLETHHAPNFMNIKVVFTTEGAKDVGRYWDFVGVKGRSSWEDREGAQAIISTNF
                     NKGDIKTESPFAVAGFTAITERWDLGSSIHELSHFNSPVRKHLRDQNLDTDLPHVIGL
                     EEVYNFFLQYITSSYEYGFGFYNVERILNEKMSQYANRFGNDERGLRLARLAFTSVLH
                     MEQIGLTRPEIMGVLGKITTVQDLIQFHFFGPEDIALIKQNVGVFVDSSREQTEKDFA
                     RAQEAVEQARQIVKIKLSLATQAEADLKRARTAFKEAWSGVAGKSQPGKEFVAAQERV
                     NVAQKRRDDANSELKTARNELEKAEKQVKQLAIVLKKADAMVAQLEKNPYVPKSDDVM
                     MNLDSSQGVLNLDWPRIRKQMDDLLADEATGFKSFMDILRDATAKEQLWMLYELKKRA
                     DSENEQASRAREFLSRFYEEPDASEILVSVVKISREVLFGQRHQQSLSRRLLDPVLTA
                     APETIRTQVSLFTGWLPAINPVLENLAGTHLAIGESHYRQKTKELVDSHNWKELEGIV
                     RGLRGSDETRAMTVSYLYRDHRYNPEVEKILSYFNTEPPVTGFGKNFSKYWKLFWSGP
                     SPVVLGVSIAISGIMLLLGVGTVVVLTGFSLYLPVHIAMLVIPTMLRTRNFEKFVENT
                     SRAIYALKGAEQPENAMEILQSLIRGIRAFAKGENGVALKGEKLRSELWDRVHYDRTA
                     LNVLAAMEKYEGTGVSLTPSLPELQDKTVSSDDARTSVEDILAIKAMSSAENRNVWGA
                     IEDELVFSDILTKEWAEALKNYKTWMQTGSVKERAIAEEAYHQLLMEAFDAYLRKALP
                     AEEADAALAQFKDVFPDRDGRSLTDKAIQAGQDTTELAKRNPGVRKYLSEKHSKPYLL
                     KQKELVLSKAQEVSTQEAVTEFFEKIENELVDGLSDWQNVRQIMSPVLSEAAQKDLDA
                     LEAAGNRKDYESTLMLALREILENKLGIAAAHEKLMGLRNSIARAQHAKADKILKRLN
                     AWSEAKKREAAQWILNNLDDTLDHLISKYGLDEETLMHARFEPNKTGVGGKIVLDPDY
                     LPEDFLIRDKNSGASEPFGRPQQYLRYTFVTEGYTEETAIHEEGHDLDNLYSFDYDGR
                     IYGIWEREDFIQKKKGLLIAEIHSFLREIYVSKKWKEASGIKDLEARKAEKKFLVDRH
                     IAKVKEEYLPLYLKKILKNLKDPLYPQFTMQFLDAIQLPSEIEKLQKEILEKSADMIL
                     KSKNENEFSNKYLATFRAPELRSELRGSVEMEKGPSFVEESESLTGLRNMALEVNGIL
                     GGPVRPETIRDISIRTASPVSDPNLKGNIGYQRTTKEGKTEVVLSDVLLERPDDTKLL
                     HYAVSVLIHEVAHAEGKDELAAYEITADALEALDAGGFELTISRMRALLRMARGEMSS
                     EVMNRKLLKDIAIQILEGGRNVHDGIETILSRLNPKVIHLGDDIPEAFEGMGLTAVAG
                     LGALEAELKRSSANHTTILMSEEFLGSLTGKPQDARKLAELLKAYPILRYGEHDRRDS
                     TVVLVAELMASSLEILREHYRLPERDEIVNGIRFRFYDAGDLKKTLADFLTEYQARAA
                     VARAA"
BASE COUNT         1646 a         1933 c         1534 g         1830 t
ORIGIN      
        1 acggatcttg ccgaaaagga aaaggccgaa ctgctcattc tcgaatcgta tctgccgaaa
       61 cagctgagcg acgaagaact tcgagctgaa atcaagaagg cgatgcaaat ctccggcgct
      121 caaaatccct ccgacatcgg gaaactcatg aaaacactca tgcctcttat ccagggaaaa
      181 gcggacggaa aaagagttca agccgcggtc aaagacctcc ttaagtagaa gccacccgca
      241 tatcagcgtc agccctcgcc ctaggagtta gtttacataa gatacattat aagacgtata
      301 atttcctgag aaagcggccc tcgcctgtat gattagatag cctcagtggt aacggaattt
      361 taacggatac agtaaaaaca tacgtgatag gagctatttc gcaggatgct tttcacgtgt
      421 taggcggcgc gggcgacggc ggcacgggcc tgatattccg tgaggaaatc agccagagtt
      481 ttcttaagat caccagcgtc atagaaacgg aaccggattc cgttgacgat ttcatccctt
      541 tcgggcaagc ggtagtgttc ccgcagaatt tcgagtgacg aggccatcaa ttcggccacg
      601 aggacaacgg tcgaatcccg ccgatcatgt tcaccatatc ggagaatagg atatgccttc
      661 aacagctccg ccaacttcct ggcatcctgc ggttttccgg tcagagatcc gagaaactcc
      721 tccgacatca agatagtcgt gtggttggcg cttgaccgtt tcagttcggc ctcgagagcg
      781 ccaagtcccg cgacggccgt aagccccatg ccttcaaagg cttctggtat atcatcacct
      841 aaatggatca ccttcggatt gagcctggag aggatcgttt cgataccgtc atggacgttt
      901 cggccgccct caaggatttg gatggcaata tccttgagga gcttacggtt cataacttca
      961 gacgacatct cccctctcgc catccggagt agcgcccgca ttctcgaaat ggtcaactcg
     1021 aaaccacccg cgtcaagcgc ttcaagggca tccgccgtga tctcgtaggc cgcaagctca
     1081 tccttccctt ccgcgtgcgc gacctcgtgg atcaggacgc tcaccgcgta atgaagcagt
     1141 ttcgtgtcat caggacgctc tagcagtaca tcgctcagca ccacttcagt tttgccttct
     1201 ttggttgttc tttgatatcc gatatttcct ttgaggttgg gatctgaaac aggtgacgct
     1261 gtccggatag aaatatcccg tatcgtttcc ggccgaacag gcccgcccaa gatcccgttg
     1321 acttcaagcg ccatattccg aagtcccgtc agggactcag attcctcaac aaacgaaggt
     1381 cctttttcca tctccaccga tccgcgaagc tccgagcgca actcgggcgc acgaaacgtc
     1441 gcgagatatt tattgctaaa ttcgttttcg ttcttcgatt tcaggatcat gtcggccgat
     1501 ttctccaaaa tctccttttg cagtttctct atctctgatg ggagttgtat cgcgtccaaa
     1561 aactgcatgg tgaattgcgg ataaagcgga tctttaagat ttttcaggat ctttttgaga
     1621 tacaaaggca ggtattcttc tttgactttc gcgatatggc ggtcaaccaa aaacttcttt
     1681 tccgccttcc gcgcttccag atccttgatc cctgaagcct ccttccattt tttcgaaaca
     1741 taaatttcac gcaaaaacga atgaatttcc gcgatcagca atcccttctt cttttgaatg
     1801 aaatcttccc gttcccaaat gccataaatt ctcccgtcgt aatcaaaaga atacaggtta
     1861 tcaagatcat gaccctcttc gtggattgcc gtttcctcgg tataaccctc cgtcacaaat
     1921 gtatagcgca gatactgctg aggtcgtccg aacggctcgc tcgcgccaga gtttttgtcc
     1981 cggattaaga aatcctcggg aagataatcc ggatccagta cgattttacc gcccacaccg
     2041 gttttatttg gttcaaatcg cgcgtgcatc aaagtttcct catcgaggcc atatttcgag
     2101 atcagatgat ccagtgtatc gtcgagattg ttgaggatcc attgcgccgc ttcccgcttc
     2161 ttcgcttcac tccaagcatt caaccgtttc aaaatcttgt ccgctttggc atgttgtgcc
     2221 ctggcaatcg agttgcgaag tcccatcagt ttttcatgag ctgccgctat tccaagctta
     2281 ttttccaaaa tttcccgtaa cgcaagcatc aacgtgctct cataatcctt cctgttcccg
     2341 gccgcctcaa gcgcgtccaa atccttttgg gcggcttcag acaaaactgg tgacattatt
     2401 tgccgtacat tctgccagtc cgaaaggcca tccaccaact cattttcgat tttttcaaaa
     2461 aactccgtaa ccgcctcctg cgttgaaact tcctgagctt tggaaagcac caattccttc
     2521 tgttttaaca agtacggctt cgagtgtttt tcagataaat atttacggac accgggattt
     2581 cgttttgcca attccgttgt atcctgacca gcctgaattg ccttatcggt cagtgaacgc
     2641 ccgtcccggt caggaaaaac atccttgaac tgagccaagg ctgcatccgc ttcttcggcg
     2701 ggcagagcct ttcgcaaata tgcatcaaag gcctccatga ggagctgatg gtatgcttct
     2761 tccgcaatcg cgcgttcttt cacggatccg gtttgcatcc atgtcttata atttttgaga
     2821 gcctcggccc attcttttgt aaggatgtcg ctgaaaacaa gctcatcttc aattgcgccc
     2881 cacacgttgc gattttccgc ggagctcatg gccttaatcg ccaaaatatc ttcgaccgag
     2941 gttcgggcgt cgtcggacga aacagttttg tcctgcaact ccggcaaaga cggtgtcaga
     3001 ctgacgcctg tcccttcata tttttccata gcggcaagca cgttcagcgc tgtccggtca
     3061 taatgaaccc gatcccaaag ctcggatcgc aacttctcac cttttaaggc gacaccattt
     3121 tctcctttcg caaatgccct gatccctcgg attaaactct gaagaatctc catggcattt
     3181 tccggttgtt ccgctccctt caacgcataa attgcgcgac tcgtgttttc cacaaacttt
     3241 tcgaaattcc gggttcggag catggtcggt atgacaagca ttgcaatgtg aaccggcaag
     3301 taaagagaaa accctgtcag cactaccacg gttccaaccc ccagcaacag catgattcct
     3361 gatatagcaa tggaaacgcc caaaacaacc ggtgaaggtc cgctccagaa caacttccag
     3421 tatttcgaaa agtttttccc gaagcctgta accggcggct ccgtattaaa ataggagagt
     3481 attttctcaa cttcgggatt ataacggtga tcccgataca gataggagac tgtcatggcc
     3541 ctggtttcat ccgatccgcg aagtccccga acgattcctt ccagctcttt ccagttgtgg
     3601 ctgtcaacca gttcttttgt cttttgtcgg taatgggatt ctccaatcgc gagatgggta
     3661 ccagcaagat tttccaacac aggattgatc gcgggcaacc aacctgtaaa caaactaacc
     3721 tgggttcgta tagtctcagg cgccgctgtc aacacaggat ctaggagccg cctgctcagg
     3781 gactgctgat ggcgctgtcc aaagagaacc tccctcgata tttttacgac cgagactaga
     3841 atctccgagg catccggctc ttcgtagaat cgcgacaaaa attcacgggc acggctcgcc
     3901 tgttcatttt ctgagtcggc ccgttttttc agttcataca acatccaaag ctgttctttc
     3961 gcagtcgcgt ctcgcaagat gtccataaac gatttaaatc cagttgcctc gtccgccagg
     4021 agatcgtcca tctgttttct aatcctcggc caatccagat tcaaaacccc ttgtgaacta
     4081 tcgaggttca tcatgacatc gtcagatttc ggaacgtacg gatttttttc aagctgagca
     4141 accatagcgt cagctttctt caaaacaatc gccagttgtt ttacttgttt ttctgctttc
     4201 tcaagctcgt tacgagccgt cttgagctca gaatttgcat catccctgcg cttctgcgcg
     4261 acgttgacac gttcttgcgc ggcaacaaat tctttaccag gctgactctt ccctgccacc
     4321 cctgaccaag cttctttgaa tgcggttctt gccctcttaa gatctgcttc agcttgagtt
     4381 gcaagggaca acttaatttt gacaatttgg cgtgcctgtt ccacagcttc ttgcgctcgc
     4441 gcaaaatctt tttccgtctg ctccctagaa ctgtcaacaa aaacacccac attttgcttg
     4501 ataagggcga tatcttcagg gccgaagaaa tgaaactgga tcagatcctg gaccgtggta
     4561 atcttcccaa gcacccccat gatttcaggg cgagtcagac caatctgttc catgtgaaga
     4621 accgatgtaa aagccagtct tgcgagtcgc aaaccgcgtt catcattccc gaatcgattc
     4681 gcatattgag acattttttc attcaaaata cgttcgacat tgtagaaacc aaacccgtat
     4741 tcgtaggacg acgtgatgta ttgcaagaaa aagttataaa cttcttcgag accaatcaca
     4801 tgcggtaaat ccgtatcaag attctgatca cgaaggtgtt ttctcacggg agagttgaag
     4861 tgggaaagct cgtggatgga cgaaccaaga tcccaacgct ccgtaatcgc tgtgaacccg
     4921 gctaccgcga acggactttc ggtcttgata tctcccttat tgaaattggt tgaaataatg
     4981 gcttgtgcgc cttcccgatc ttcccaagaa ctgcgccctt tcaccccgac aaaatcccag
     5041 tatcggccga catcttttgc gccttcggtt gtaaaaacga ctttgatgtt cataaaattt
     5101 ggagcgtgat gtgtttcaag ccagcgctca ttcgccgcaa tcctctgttt gcgttcggct
     5161 tcagattcct caagacgtcg cgacgcttcc ggaaacccgc cggagacggc gggattgtaa
     5221 gcaatttccg ccgttgtacc ggcctcaatg ctgagctgac cataatgcaa attctttttg
     5281 atcacctgga taagcccttt tcgatccctg ttgctttttt cgatccgtga ggcgccatga
     5341 ttggcaagaa tcgcatccgc cagatctgtc gccagccggc tttccagggc ctcaatcatt
     5401 tgacggattt tgatcttttc gacgatttgt ttggcccctt gaagtttgag aataagttct
     5461 tgccgttcat ccacaagttt tttgatctgg cgaaccgcgt cagcttctat ttcatcctgt
     5521 tcatagggcg cgggtttttc gagaagggag atcacttcgg atgtaatgtt gggattgcca
     5581 agtaacgcgg cacgcgcgcc cgcgatatcc tgccatctcg cagctccact cgttgtgtcg
     5641 cgaaggacgg actggatctc ctcccggatt ccttttctga attcagactc ccgcttccag
     5701 gctgaagcaa aaattcccgt ttccaccgcg ccttgcccta atatccgggc cccgaggtag
     5761 cagatccaaa tcacgcctgc cgctattcca aaatacagga aagatcctcc gagcaggatt
     5821 gaggaaaccg cgcctcccgc gacccacaga gaagaactgg gccctttgaa aaacattttt
     5881 ccaggatggc gcagattttg gagtaactcc atcggttgcc agaagacaaa aatgagcgcc
     5941 gtaccgacaa aaggaagcgt tgctggaaaa gcaacggcca aagcaaccgt ccctgcaccg
     6001 atcgtagcaa aaaacacctg tcgcagaccg gtgcccgcgg tagtttcagc gatgaatgtc
     6061 cggatcgccg tcgcgaggtt gcgccgattc tcagcgaccc caagatttga aatgaccgag
     6121 cggtctttgg cccagaattg tctgctcgta acgctctggc gcgcgagcgc cgcctgatcc
     6181 ttgacttcgc gggcattagc catgatcagg gccagaacat ccatcacggc gtatttaatt
     6241 cgctcgaaca agaacccgta ttgtttgatg acgataccgt ttttctgatc aggagacctc
     6301 gcgataccgg ttcgtgaaaa gtgaccggat ctcataccct gatacgccaa catcttgttt
     6361 tcggtgacgc taatatcatt tgaagcacag aaataaactt ccgaattctt tccgactcgg
     6421 aggctcccct cacgctcggc acggcgaaca aacatgacgc gctcatccct gtcaacgcga
     6481 accggtgaca tgaccgtatc ggaaacacgc gccccatctt gaatctggat gtccccgcgc
     6541 cagtggacgc gttccagcca cgcgccattc ccgatcgaac acccatcctt cgtcgcgata
     6601 agttcgcccg aaattagcac aaacggcccc atcttttcaa aactgccggt tttttcctcg
     6661 gtttcttttt tcatcgcgat gatactttct accttttcat ccgcaatttt tgcttcattc
     6721 gaatcgtttc caaatttctc tcgcgccaca tcacgttctg actgagccgc gtcacgagct
     6781 tttccgattt ccgcaagacg tttctctttc tcggtcgtta ctgtcccatt cacaattaaa
     6841 gttccggccg cgagcgagtc ctcagccccg gcaatgttgc agtgaccgac caggataacc
     6901 ccacgatccg ttaacttgac cggtagcgtg atcccttgcg aat
//
LOCUS       DAOSIO010000073         6930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_9921_length_6930_cov_4.155636, whole genome shotgun sequence.
ACCESSION   DAOSIO010000073 DAOSIO010000000
VERSION     DAOSIO010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9921_length_6930_cov_4.155636"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            508..1182
                     /locus_tag="PK590_03875"
     CDS             508..1182
                     /locus_tag="PK590_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23766.1"
                     /translation="MREIILMTLSLLMATQAQPAEAFWSKKKEPLMERAEKYYENREY
                     YRAREATFEILAENPNHEAAQALMGKILDKEIERQKEQVYPMAVEEMDSDDRSSEIKT
                     WLERSNALLARKEYDLALFAAEKVFIFDAENRQASELIDRIKERAIKEGKADVLFLGK
                     MYKEEIAERLSLYRDRARELARDGHFGQARFTVEKILLLEPEDPQALSLYQNVLEQEE
                     AFRHEA"
     gene            1172..1414
                     /locus_tag="PK590_03880"
     CDS             1172..1414
                     /locus_tag="PK590_03880"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOG23767.1"
                     /translation="MKREKLYKIGEVMQYTNLSRQTLHNYTVAGLIHEARRTISGHRL
                     YDETTFDRLEQIKILQSKNYTLTQIKKILEQQDGQK"
     gene            1460..2239
                     /locus_tag="PK590_03885"
     CDS             1460..2239
                     /locus_tag="PK590_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOG23768.1"
                     /translation="MNQDEVNMWKKYKRTKSLIVREEIVKKYLYLVKYVAGRVAVGLP
                     PNVEFNDLVSYGILGLFDAITKYDVSQGNKFETYAVARIRGSIMDELRKLDWAPRLLR
                     KRAREIDRKCKELEEKYGRLATDDELAKALHMSVDDLNSIYSDLSSTSFLSLDEVWQN
                     DEGNKPISRLQTIEDSLITNQFSFVHQAEVKDLLADAIDQLPEKEKLVIVLYYYENLT
                     LREIGEILDVSESRVCQIHTKVVTRLRGHLMKRAGHLTMEV"
     gene            2419..2667
                     /locus_tag="PK590_03890"
     CDS             2419..2667
                     /locus_tag="PK590_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23769.1"
                     /translation="MNSVVGKKKSFKIQAPIERSAQSAERDEVRKYLRQIPGESEPNL
                     GRVREIKEEIKKGTYLTREIIEEAAMQLALRLLKKDSH"
     gene            complement(2609..3925)
                     /locus_tag="PK590_03895"
     CDS             complement(2609..3925)
                     /locus_tag="PK590_03895"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOG23770.1"
                     /translation="MFRSFFIFFILVTFLTPCQAEAYHPTPEEIELITKIEKDTLQYF
                     IRLSDKNTGLTRDSSRSGSPSSIAATGFALAAFAIGGSRGWIPHDYAYNRVLATLKVL
                     KEDAQHQNGFFYHFLDARSAKRVWSSEASSIDTALLLAGALLAGQYYPGTEVETIAKE
                     LYERVDWKWMMNGSKFICMGWTPESKFLPYYWDSYNELMLLEALAVGAPQNPAPPETW
                     HAWLRPEGNYNGHRVIYASSGSLFTYQYSHAFIDFRGLKDDGNDFFDNSREATLANRD
                     FSLSFAGKYRGYSENSWGLSASVGPGGYKAYGSLPGGATHDGTIAPYAAISSIVFTPE
                     LSIAALKFFYNNYAENIYGNFGFKGAFNLDKSWWADEYLGIDQGITLLMLENFLNDGI
                     VWKKFMSLDAIQKWIALTGLAGKSDENASESPSLEVATPTASPLPQ"
     gene            complement(3918..4562)
                     /locus_tag="PK590_03900"
     CDS             complement(3918..4562)
                     /locus_tag="PK590_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23771.1"
                     /translation="MKGNRRRHFFINKPLQLRYMLSVTVPLLLICCVAIFGFYIGIWG
                     SVLGAFTNEAMRDDLLTASRMVEYEQARYQTAHEGFSLLSLFKETEKLGQRQREVFHE
                     ILNETNRAIAWKLLLLLVLTAWGTIYVSHKIAGPLYRFSKTFSDVERGNYRTRIFLRK
                     GDEGHPVAKEFNAAIESADRLLSDLKTAAQNPDQAQALATIKAKLDPIKTSADV"
     gene            4688..6571
                     /gene="glmS"
                     /locus_tag="PK590_03905"
     CDS             4688..6571
                     /gene="glmS"
                     /locus_tag="PK590_03905"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOG23772.1"
                     /translation="MNYTVRLTSPETNMCGIIGYIGYRDVVPVLIQGLEQLEYRGYDS
                     AGIACLDSRKGNLFLAKEKGKLAALKEKIGDFNIDSHIGVGHTRWATHGEPSRENAHP
                     HTDFNGQIALVHNGIVENYATLKKTLQKKKVRFTSQTDTEVGVQVIGRFYKKKTSLFQ
                     AVKQAIPQLRGFFAFVLFSKDDPETIIAYKRSNPLVIGLGQGENFVASDVTALLSYTR
                     RVIYLEDEDIAVLTRNKVTVYSLKRHSWVSRPVTTVNWNVAQAQKQGFPHFMLKEMYE
                     QPEVLRNILSKRIDRQGEVYFDTITKAFRKRLKKIERVFMISCGTAYHAGLVGGTAIQ
                     QYARVPYQAQVSSEFRYTNPMVGPEDLVVLVTQSGETADTLAALREAKSKGALVLAIV
                     NVVGSTIAREADGVIYTHAGPEIGVASTKAYVAQLATLLLLAFYMGRLRRKLSRDAHR
                     TLIREFKKIPDQAAKILKEHKRFESCAKKHYTRRNFLFLGRGYNFPTALEGALKLKEI
                     SYAHAHGYAAGEMKHGPIALIDHEQPVICIAPDSLTYEKMISNIQEICARDGIVIAIG
                     TEGRKDLEKMADDFFCIPKTAEVFSPILTVIPLQFFSYQMAVLNGRDVDQPRNLAKSV
                     TVE"
BASE COUNT         1885 a         1555 c         1777 g         1713 t
ORIGIN      
        1 tagtctttgt tccgggattc ccattaatgc gatttcctgg atctgacgag tgtgtttcgg
       61 ataacgctta atgagcttaa gggttggaac accctgcccg aatttcaagt caattaacct
      121 ttcgtagatg gtcatagttt gtataccctc aaaatagtcg gacttaaatt atttttattc
      181 tcgccaaaga aagttatcgt ctttttcata aaataaatga gagctcgggg aggttttccg
      241 acctactcat cccgcgagac gccctgttgt atagggcgac accgaagggc ttttccaatg
      301 agatctttgg actttgccat caaggggttc cgatcagacc agcttcctca aaaaaatatg
      361 cggattgatt agctatatat agcatattgt ttaataccga cacacaataa gttgtgttcc
      421 attaaaatat tttaaaaata ctgttgactc agtttgaaaa aattatagat tgtaatctat
      481 aaaatgtaat ttacatttta taaacctatg cgcgagataa ttttaatgac cctatcgctt
      541 ttaatggcaa cgcaagccca accggccgaa gctttctgga gtaagaagaa agaaccgttg
      601 atggagcgtg ccgagaaata ttatgaaaac cgcgagtatt atcgcgcgcg cgaggccacc
      661 tttgaaattt tggcggaaaa tcccaatcac gaagccgcgc aagccttgat ggggaaaatt
      721 cttgataagg aaattgagcg tcagaaggaa caggtttatc cgatggcggt ggaagaaatg
      781 gattcggatg accgaagttc cgaaatcaag acatggctcg agaggagcaa cgccctcctg
      841 gcccgtaagg aatatgatct ggctcttttt gccgcggaaa aggtttttat atttgatgcg
      901 gagaaccggc aggcttcgga gctgatcgac cggatcaagg aacgggcgat caaggagggg
      961 aaggcagacg ttctgtttct tggcaaaatg tataaagaag aaatcgcgga gcgattgagt
     1021 ctttatcgtg accgggccag agagctggcg cgggacggac atttcggaca ggctcgtttt
     1081 accgttgaaa aaatcttgtt gcttgagccc gaagatcccc aagccctaag tctctaccag
     1141 aatgttctgg aacaggagga agcgtttcgc catgaagcgt gaaaaacttt ataaaatcgg
     1201 tgaggtgatg caatacacga acctttcccg ccaaacctta cataactata ccgttgccgg
     1261 ccttattcat gaagcgcgtc gtacgatatc tggtcatcgg ctttatgacg aaacgacctt
     1321 tgatcgtctt gagcaaatca agatattgca gtccaagaat tatacgctaa cacaaatcaa
     1381 gaaaatactg gagcaacagg acggtcagaa ataaagatca gcctggtttt tataaaaaag
     1441 gaaaaaagga gaccctgata tgaatcaaga cgaagtgaat atgtggaaga aatacaaaag
     1501 gacgaagagc cttatcgtcc gtgaagagat tgtaaaaaaa tacctgtatc tcgtgaagta
     1561 tgtcgccggc cgcgtggcgg tcgggcttcc tccgaatgtt gagtttaacg atctggtcag
     1621 ttatggaatc ttggggcttt ttgacgcgat cacgaaatac gacgtctccc aggggaacaa
     1681 gtttgagact tatgccgtcg cgcgtatccg gggttctatt atggacgagc tccgaaagct
     1741 tgactgggcc ccgcgtcttc ttcgtaaacg cgctcgtgag atagaccgga aatgcaagga
     1801 attggaggaa aagtacggac gcttggccac ggacgatgaa ttggccaaag cgcttcatat
     1861 gtcggttgat gaccttaatt ccatttacag cgatctgagc tccacgtctt tcttgtcgct
     1921 ggatgaggtc tggcaaaacg atgaggggaa caagccgatc tccaggcttc aaacgatcga
     1981 agattcgcta atcaccaatc agtttagttt tgtgcatcag gcggaggtca aagatttact
     2041 ggccgacgcg atcgaccagc ttcccgaaaa ggaaaaactc gtgatcgttc tctactatta
     2101 cgaaaacctg acccttcgcg aaatcggcga gatcctggat gtgtccgaat cccgcgtgtg
     2161 ccagatccac accaaggttg tgacgcggtt gcggggacat ttgatgaaga gggccgggca
     2221 tttgacgatg gaagtgtaag gaactatcga ccttttaact tttacaaaac aaataaagat
     2281 ttcgacacgc tgggccgata aacataaggc cgtagcaaag gacgcgttga aataaacgtc
     2341 cgggtcctag gttggacggc aaaattttta tattttcaaa aattttacga tgccaattta
     2401 ggcttaaagg cggaaagaat gaacagcgtg gtcggaaaaa agaaaagctt taaaatccag
     2461 gccccgattg aacgaagcgc ccagagcgct gagcgggacg aagtgcgcaa gtacctccgt
     2521 caaatccccg gtgagtctga acccaacctg ggtcgtgtcc gcgagatcaa ggaagaaatc
     2581 aagaagggaa cctacctgac ccgtgagatt attgaggaag cggcgatgca gttggcgttg
     2641 cgacttctaa agaaggactc tcactagcgt tttcgtcgct ctttcccgcc aaacccgtca
     2701 aggcaatcca cttttgtatc gcgtcaagcg acataaattt tttccagaca atcccgtcat
     2761 tcagaaaatt ttccagcatt aaaagggtaa tcccctgatc gatccccaaa tattcgtcag
     2821 cccaccagga tttatcgaga ttgaaggccc ccttgaaacc gaaatttcca taaatgtttt
     2881 cggcgtaatt gttgtaaaaa aatttcaggg cggcgatgga aagttcgggg gtgaagacga
     2941 tggaagagat tgccgcgtag ggagcgatgg tgccatcgtg ggtggcgcca ccggggagcg
     3001 agccgtaagc cttatagccc cccgggccga cagacgctga aagtccccat gagttttccg
     3061 aatagccccg gtatttgcca gcgaagctta atgaaaagtc gcgatttgct aaagttgctt
     3121 cccttgagtt atcgaaaaaa tcattcccgt catctttaag gcccctgaaa tcaataaaag
     3181 cgtgggagta ctgataggta aaaagggagc cggatgaggc gtaaatcacg cgatgcccgt
     3241 tataatttcc ttccggcctg agccacgcgt gccaggtctc aggaggggcg ggattttgag
     3301 gagccccgac ggcaagcgct tcaaggagca tcagctcgtt gtaagaatcc cagtaataag
     3361 gaaggaattt cgattccggc gtccagccca tgcaaataaa tttcgaacca ttcatcatcc
     3421 acttccagtc gactctttcg taaagctctt tggcgatggt ctccacttca gttcccggat
     3481 aatactggcc agcgagcaag gctccggcca aaaggagagc ggtatcaatc gaggaagctt
     3541 cggatgacca cacccgcttg gctgatcgcg cgtcaagaaa gtgataaaaa aagccgtttt
     3601 ggtgttgcgc gtcttctttg agaactttga gcgtggcaag aactctgtta taggcgtagt
     3661 catgagggat ccacccacgc gatcccccga tcgcaaaggc cgcgagagcg aatccagtcg
     3721 cggcaatcga agagggcgat cccgatcgcg aagagtcccg ggtgaggccg gtgtttttat
     3781 cggacagccg aataaaatac tggagggtgt ccttttcaat cttggtgatc agctcgatct
     3841 cttcaggggt gggatgatag gcctccgcct gacaaggtgt gagaaaggtg accaggatga
     3901 aaaaaataaa aaaagaccta aacatcagcg ctggttttta tgggatcgag ttttgcttta
     3961 atggttgcca gggcttgagc ctgatcggga ttttgggccg cggttttcag gtctgacagc
     4021 aggcggtccg cggattcgat cgcggcgtta aattccttcg ccaccggatg accctcgtcg
     4081 ccttttctta gaaaaatccg ggtacggtaa ttaccgcgtt caacgtcgga aaaggttttg
     4141 ctaaatcgat acaaagggcc ggcgattttg tgggaaacat aaatagttcc ccaggcggta
     4201 agaacaagca ggagcaacaa tttccaggcg atggcgcgat tggtctcgtt gaggatttca
     4261 tgaaagactt cccgttgccg ctgtccaagt ttttcggtct ctttaaacag ggaaagaagg
     4321 gaaaaccctt catgagcggt ctgatagcgc gcctgctcat actccaccat ccgcgacgcg
     4381 gtgagaaggt cgtcgcgcat cgcttcattg gtaaacgctc cgagaacact gccccagatc
     4441 ccgatgtaga atccgaaaat agcgacacag cagatcagga gtaatggtac ggtaacgctc
     4501 aacatgtaac gcagttgaag aggcttattg ataaaaaaat gtcgacggcg attacctttc
     4561 atcgtgaaac cctcctgcgc ctatcttatg ggaatgataa tcatctgtca aaattaagtt
     4621 gcttggatat aatagcgcat atcgcgtgcg gagtacggag aaatgaaggg ttttttacaa
     4681 ttaccgtatg aattacaccg tgcgcctcac ttcgccggag acgaacatgt gcggcattat
     4741 cggatacata ggttatcggg acgttgttcc cgtgctcatt cagggattgg agcaactcga
     4801 atatcgcggg tatgattccg cggggatcgc ctgcctcgat tctcgaaaag ggaatctttt
     4861 tctggccaaa gagaaaggga agctggccgc tctgaaggag aaaatcggag atttcaatat
     4921 cgattctcac attggggttg ggcacacccg ttgggccacc cacggagaac cttcacgaga
     4981 aaacgcccac ccccacacgg attttaacgg gcaaatcgcg ttggtgcata atgggatcgt
     5041 tgaaaattac gcaacgctca agaaaacgct tcagaagaaa aaagtccggt ttacttccca
     5101 aacggacacc gaggtcggcg ttcaggtgat cggacgtttt tataaaaaga aaacctccct
     5161 gtttcaggcg gtgaaacagg ccattcccca gctccgcggt ttttttgcgt ttgttctttt
     5221 ttcgaaagat gacccggaaa cgattatcgc ttacaagcgt tctaatcctc tcgtgattgg
     5281 ccttggacag ggagagaatt ttgtcgcgag cgatgtgacc gcgcttcttt cttatacgcg
     5341 tcgagttatt tatctggaag atgaggatat cgcggttctg acacgaaaca aggtcacggt
     5401 ttattctctc aaaaggcatt cctgggtgag tcggcctgtc acgaccgtca actggaatgt
     5461 cgcccaggct cagaaacagg gctttcccca cttcatgttg aaggaaatgt acgagcagcc
     5521 cgaagtttta aggaacatcc tctcgaaaag gatcgacaga cagggtgaag tgtattttga
     5581 caccatcacg aaagctttca ggaaaagact caaaaaaatc gaacgggttt ttatgatcag
     5641 ctgtggtacg gcctatcatg ccgggcttgt gggtggaacg gcgatacagc aatatgcccg
     5701 ggtgccttac caagcgcagg tatcgagtga gttccgttac acgaatccaa tggttggccc
     5761 cgaagatctc gttgtgctgg tcacgcagtc aggagagacg gcggatacgc tggcggcgct
     5821 ccgcgaggca aagtcgaagg gagccttggt ccttgcgatc gtaaatgttg tcgggagcac
     5881 gattgcccgt gaagcggatg gcgtgattta tacacacgcc ggcccggaga tcggcgtcgc
     5941 ttccaccaaa gcgtatgtgg cgcaactcgc gacccttctc cttctcgcgt tttatatggg
     6001 aaggttgcgc cggaaacttt ctcgggacgc gcacaggacg cttatcaggg aatttaaaaa
     6061 gataccggat caagcggcaa aaattctgaa ggagcataag cgattcgaat cttgcgccaa
     6121 aaaacattac acgagaagga atttcctttt tctggggagg ggatacaatt ttccgaccgc
     6181 ccttgaaggc gcactgaagc ttaaggaaat ttcctacgcc catgctcacg ggtatgccgc
     6241 gggagagatg aaacacgggc cgatcgcgct tattgaccac gaacagccgg tgatttgtat
     6301 cgcgcccgat tccctgacct atgaaaaaat gatttctaat atccaggaaa tttgcgcgcg
     6361 cgacgggatc gtgatcgcga ttgggacgga aggaagaaaa gatcttgaga aaatggcgga
     6421 tgattttttt tgtattccaa aaacggcgga agttttttct ccgattttaa ccgtgattcc
     6481 gcttcagttt ttttcctacc agatggctgt tttaaacggt cgcgatgttg accaacccag
     6541 aaatctggca aaatccgtta ccgtggaata gcttaactcc ttgccatata gcgggttaca
     6601 aacacttgtc tcattcctga aaaaatttac aaggaccctt gtaataagtc tttgaggcgt
     6661 gtacctttct gtccggggtt ttgggaaaaa atatttaaaa aaatttcaaa caaaagcttg
     6721 acagaatttc tttcgcgact ataatccccg tcaccctcgg gaacgggtga tttgagagtt
     6781 atccggtttt tattctttgt aaatctagat agttccgatt ttttcagatt attcctttct
     6841 gaacgttcct catgcagggg gatttttgtc ttcagaaggt tgttctttca caaagttttt
     6901 tcgtgcggat tgccgaacgt aagtctttgc
//
LOCUS       DAOSIO010000074         6902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_9969_length_6902_cov_4.816854, whole genome shotgun sequence.
ACCESSION   DAOSIO010000074 DAOSIO010000000
VERSION     DAOSIO010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9969_length_6902_cov_4.816854"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PK590_03910"
     CDS             complement(<1..342)
                     /locus_tag="PK590_03910"
                     /inference="COORDINATES: protein motif:HMM:NF015412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOG23773.1"
                     /translation="MRKFKVGIIGCGAIGTALAKVLIKDKKLNFRAVLTAVADKNPEQ
                     AEKLYRRIRRRLPLVSIREIVKRCDFVIEAAHPSISPAVASLALRHDKYVLIMSAGGL
                     LNFPFKRLRALL"
     gene            complement(339..695)
                     /locus_tag="PK590_03915"
     CDS             complement(339..695)
                     /locus_tag="PK590_03915"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006555782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOG23774.1"
                     /translation="MQKQLLSGKIHRARVTEARIDYEGSIAIDSQLMKGAGILPYEKV
                     LIANLNNGSRIESYAISAPAGSGVVCLNGGAAKHGKVGDVLIIMTFGVYAGRELKNHK
                     PKVVYVGKGNRAIRKK"
     gene            complement(774..1628)
                     /gene="panC"
                     /locus_tag="PK590_03920"
     CDS             complement(774..1628)
                     /gene="panC"
                     /locus_tag="PK590_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOG23775.1"
                     /translation="MIRSSKKLYRILEVCRQKRKTVGFVPTMGALHEGHLSLVRASRR
                     ENRVTVVSVFVNPTQFGPKEDFSRYPRVLSHDKRLLSECGADYLFYPSVQEMYPEGLL
                     KPLKIPGAKWPVLAKGLCGKFRPGHFQGVVTVVAKFLEMIKPTRLYLGAKDYQQARVL
                     TRMVEDLSLKVKVRVMPTVREKDGLAMSSRNRFLTPEERARATMISRVLFDLRKGLKK
                     RQKTIRELKKQALRELERHVDKVQYLEVVDADWLVPARAYGGDLAALTACFVGKTRLI
                     DNVIIHAS"
     gene            complement(1723..2187)
                     /gene="folK"
                     /locus_tag="PK590_03925"
     CDS             complement(1723..2187)
                     /gene="folK"
                     /locus_tag="PK590_03925"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOG23776.1"
                     /translation="MPLVFLGVGSNLGDREARLDEAAERLTATPGIGHLRFSPVYETD
                     PVDASGGKFLNAVWSFETRMRPREVLAVLQTLEVKAGRVRKARNEPRPLDLDLLTYGD
                     AIIREDGLIVPHPRMHERYFVLKPFCDLAPEWIHPELGQTARDLLLKVEGMD"
     gene            complement(2219..3406)
                     /locus_tag="PK590_03930"
     CDS             complement(2219..3406)
                     /locus_tag="PK590_03930"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOG23777.1"
                     /translation="MFKTPLAIEPTSRLQKLPPYLFAEIDRKKRELIAKGKDVIDLGV
                     GDPDIPTPDFIVHAMQKAVENPRNHRYALDLGMPEFRLAIRDWFERRFHVKLDPDKEI
                     LPLIGSKEGIGHLPLAVINPGQVCLIPDPGYPVYRSGTLFAEGIPYTMPLLEKNHFLP
                     ELHLIPGDVLKNARLMFLNYPNNPTSQVADKSFFAEVVEFAHKHKILIAHDAAYSEMT
                     YDGYKAPSILEVPGAKEVAIEFFSLSKTYNMTGWRVGFAVGHPQAIALLGKVKSNLDS
                     GIFQAIQLAAITALKDGDSALEENLKIYQGRRDLLIQGMNNLGFKTALPRATFYCWIP
                     VPPGYTSSELSLKFLEEMNIVVTPGNGFGPNGEGYFRISLTVAESRLLEALTRVKKSY
                     SRS"
     gene            complement(3554..4345)
                     /locus_tag="PK590_03935"
     CDS             complement(3554..4345)
                     /locus_tag="PK590_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23778.1"
                     /translation="MRDKSMEQKKPGQNFLFFQLNSEERESAIYYFLEAILRVRDSRP
                     GLSGAGFFDEDVNIYLAHLLFAMSLPEYHDMADPFISSDPGEVSEWIRQTDDPMPRYF
                     IYKVNADNLLVKNTLFAEKPGEVNRVICRKTEKGTNRLAVAYYDHAARCHKGIYHKKT
                     GVGQVLDKISGRYELYRKILLRIREDYFQFIHCFREQAFDHFFAQMNRYERFIRKDDL
                     LDQFLGVYQSWLVSKVPESRDQARALAMELKKIDPEFRFDLSKLS"
     gene            complement(4514..5272)
                     /gene="recO"
                     /locus_tag="PK590_03940"
     CDS             complement(4514..5272)
                     /gene="recO"
                     /locus_tag="PK590_03940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOG23779.1"
                     /translation="MSIHKVEGVVLKNQPFRSSSLIVTLFTREAGKIRGVVKGVHREG
                     EIRQAGFEIFTRAEIVFYEKKRSDLHLITESAILESYHSIRDNLETIAYGSFLCELVD
                     ELTEIQDPHPAILELLLTAFHYLSVIPPEKLTTVFTVKFFGEMGLIPYTESCVSCNAK
                     PIERGYFSIKQGALLCDGCRSRDQGAPAISPAGLELLRTLSRMDMTEGLRQPHSAHAF
                     TEVRRLITQFLNYRTGKVLRSRRFIDSIRPALSK"
     gene            complement(5279..5785)
                     /gene="ybeY"
                     /locus_tag="PK590_03945"
     CDS             complement(5279..5785)
                     /gene="ybeY"
                     /locus_tag="PK590_03945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOG23780.1"
                     /translation="MPRFRDDEEGLEIQVLNECPHPGVSEKKLKKVAARILRDLGFKK
                     ALVSILLVPDAKMRALNRKYLKHAWSTDVLAFGQGKIAGKKRGARKENAGDNSDFLGD
                     IALCVPTIEAQADEYGHSFEEELDYCLCHGILHLLGHEDKTRRTKNRMLLKQKQILKR
                     VWSPSSGT"
     gene            complement(5789..>6902)
                     /locus_tag="PK590_03950"
     CDS             complement(5789..>6902)
                     /locus_tag="PK590_03950"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOG23781.1"
                     /translation="LIHSRIGILGGVMMTVISGFLSGFSVDLLLATLMASLATAFLAY
                     RVRKRSHFLRIGLGIGCTYFLVIAGFQLTQSTPWRDALQLGFLGFLNALLIAVTSYLM
                     LPVLEHFFDVVTDVTLLELSDLNHPLMRKMMVEAPGTYHHSLVVSSLAENACERIGAN
                     ALLAKVGCYFHDIGKLEKPQFFTENQGYLYMNQHDDLPPRVSFEIIINHVRDGVRIAR
                     KYRLRRAVVDFIREHQGTGVVYYFFKKAMDMAAPSDRVRADDFRYPGPKPQTRETAVA
                     LLADSAEAATRSLQELTPETIRQLVRKIINDKFIDGQLDECELTLRDLHKIQSSFVRN
                     MTAMFHTRMKYPTVDRPLSAPDIFGADQFSKFRADE"
BASE COUNT         1653 a         1854 c         1732 g         1663 t
ORIGIN      
        1 gaggagagcc cgaagccgtt tgaacgggaa attcaggagt ccgcccgcgc tcataatgag
       61 aacgtattta tcgtggcgaa gcgcgagcga cgcgacggcc ggggaaatgc tcgggtgggc
      121 cgcttcgatc acaaaatcac accgtttgac gatttcacgg atcgacacaa gcggaagccg
      181 tcgacggatc cggcgataaa gtttttcggc ctgctccggg ttcttgtcgg ccaccgcggt
      241 caaaacggcg cgaaagttca gttttttatc tttaatcaat actttcgcga gggccgtccc
      301 aatggcgcca caaccgataa ttcctacttt aaattttctc atttttttct gatggcccga
      361 ttccccttac cgacataaac aactttaggt ttgtgatttt taagttcccg cccggcgtaa
      421 actccgaacg tcatgataat gagaacatca ccaaccttgc catgcttcgc ggcgccgccg
      481 ttcaggcaaa ccacgcccga acccgcgggc gcggaaatgg cgtaactttc aatccgtgac
      541 ccgttattga gatttgcgat tagaactttc tcgtaaggca ggattccggc gcccttcatc
      601 aactggctgt cgatcgcgat acttccctcg taatcaatac gcgcctcagt cacccgcgcg
      661 cgatgtattt ttccgctcaa taattgtttt tgcatatcat ccccgttaga aatccactcg
      721 ctccgtctcc cgcggagaac ctccctccac gggccaatct tcgtctgatc ctgtcaggaa
      781 gcgtggatta taacattatc aatcagcctt gtcttgccga caaaacaggc cgtaagagcc
      841 gccagatctc ccccataagc gcgagccggc acaagccaat cggcatccac tacctcaaga
      901 tactggacct tgtccacatg ccgctccagc tctcggagcg cctgtttctt cagctcacgg
      961 atcgtttttt gccgtttttt gagtcctttc cggaggtcaa aaagtacccg cgaaatcatc
     1021 gtcgcgcggg ccctttcctc cggagtgaga aaacggtttc gggaactcat ggcaagcccg
     1081 tccttttccc tgaccgtcgg cataacccga accttcacct tgagtgacaa atcttccacc
     1141 atgcgggtta aaaccctggc ctgctgataa tccttcgccc caagataaag gcgcgtgggt
     1201 tttatcatct caagaaactt cgccaccacg gtgacgacac cctggaaatg tccgggacga
     1261 aatttgccgc aaagcccttt ggcaagaacc ggccatttcg cgccggggat tttgagcggt
     1321 ttcagtaatc cttccggata catctcctgt actgacggat agaaaagata gtccgctcca
     1381 cattccgaca acaaccgttt atcatgactt aaaacgcgcg gataccggct aaaatcttct
     1441 ttcgggccaa actgggtggg attgacaaaa acgctcacca ccgtcacgcg attttcccgc
     1501 ctggacgcgc gcaccagcga aagatgtcct tcatgcaaag cgcccatggt cggcacaaac
     1561 ccgacggttt ttcgcttctg gcgacaaact tcaagaatgc gataaagctt ttttgaactt
     1621 cgaatcatgt tcacggtatc acctcgaatc aatcaaaaca aacatctgga aggcgcttgg
     1681 gcatttcttc ttccccggcg aaacaggcgc cgcgcacggc cttcagtcca ttccctcaac
     1741 cttcaaaagc aagtccctcg cggtttgtcc cagctccgga tggatccatt cgggagcgag
     1801 gtcacaaaaa ggttttaaaa cgaaatacct ctcatgcatc ctcggatgag gaacaatcag
     1861 gccatcttcc cggataatcg cgtctccgta ggtaagaagg tcgagatcca gcggacgggg
     1921 ctcattccgg gcctttcgaa cccgccccgc cttaacttcc agcgtctgca aaaccgccag
     1981 aacttcccgc ggcctcatgc gtgtttcaaa actccagacc gcattgagga attttcctcc
     2041 ggacgcgtcc acaggatcgg tttcataaac cggagagaat cgcaaatgcc cgatgccggg
     2101 cgtcgccgtc aacctttcag ccgcctcatc cagcctggct tcgcggtccc ccagattgga
     2161 cccaacacca agaaagacaa gcggcatgat cgtcacacgg aaagacgtga acgcggagtc
     2221 atgaacggga ataacttttt ttgacgcggg tcaacgcttc caaaagacgg gattccgcga
     2281 ccgtcaggga aatcctgaaa tatccctcgc cattcgggcc aaatccgttt ccgggcgtca
     2341 ccacaatatt catctcttca agaaacttca gggaaagttc ggacgaagtg tacccgggag
     2401 gcaccggaat ccagcagtag aaagtcgctc gcggaagcgc ggtcttgaaa ccaaggttgt
     2461 tcatcccctg gatcaaaagg tcccgccgtc cttggtaaat tttgaggttc tcttccaggg
     2521 ccgagtcgcc gtctttcagg gccgttatcg cggccagctg gatcgcctga aaaattccgg
     2581 agtccagatt tgatttcacc tttccgagca aagcgatcgc ctggggatgt ccgaccgcga
     2641 atcccacgcg ccagcccgtc atgttataag ttttggaaag cgaaaagaac tcgatcgcga
     2701 cctctttcgc gccggggacc tcaagaatgc tgggggcctt gtatccgtca taggtcattt
     2761 ccgaataggc cgcgtcgtgc gctatcaaaa ttttatgctt atgggcaaac tccaccacct
     2821 ccgcgaaaaa actcttgtcc gcgacctgag aagtcggatt gttgggataa ttcaggaaca
     2881 tcaacctcgc gttcttgagg acatccccgg gtatcagatg gagctccggc aagaaatgat
     2941 ttttctcaag aagcggcatg gtataaggaa ttccttccgc aaaaagggtc cccgaacggt
     3001 acacgggata gccagggtcc gggatcaagc acacctgtcc cggattgatc accgcgagag
     3061 gaagatgtcc aatcccttcc ttggagccga tcagcggtaa aatttccttg tccggatcga
     3121 gcttgacgtg aaatcttcgt tcaaaccagt cacggatggc cagacgaaat tccggcattc
     3181 ctaggtccag ggcgtagcga tgattcctcg gattttcaac cgccttttgc atggcgtgga
     3241 caataaaatc cggagttgga atatcgggat ctcccacgcc aagatcaatg acatctttcc
     3301 ccttggcaat taattcgcgc ttttttctgt caatctccgc gaagaggtag gggggcagtt
     3361 tctgaagccg gcttgtcggc tcaatggcaa gcggtgtttt aaacataaat ttcctctctc
     3421 gatttcatgg atgattaaaa gggcttattc ccgaacggta tttatgggcg gaaattatat
     3481 cagactttat cctttttgtg ggactcgtca cgttcccgaa atacgcgaga ggagcttgag
     3541 gctctttgac cggtcaggac aatttggaga ggtcaaagcg aaattcaggg tcgatttttt
     3601 ttaactccat cgccagggcc cgcgcctgat cgcgcgactc ggggactttg ctgacaagcc
     3661 aggactgata caccccgaga aactggtcca gcaaatcatc cttccggata aaccgctcat
     3721 aacggttcat ctgcgcgaaa aaatgatcga aggcttgttc cctgaaacaa tggataaact
     3781 ggaaataatc ctccctgatt cgtaaaagta ttttccgata cagctcatag cgtcctgaaa
     3841 tcttgtccaa aacctgtccc acgcccgttt ttttgtggta aatccccttg tggcaacggg
     3901 ccgcgtgatc gtagtacgcc acggccaggc ggttagtccc tttttccgtt ttccggcaaa
     3961 tcacccggtt gacctctccc ggcttttcgg caaacagagt gtttttgacg agtaaattgt
     4021 cggcattcac cttgtagata aaataacgag gcattggatc atcggtctgg cgtatccact
     4081 cggacacttc accaggatcg ctcgaaataa aaggatccgc catatcatga tattcgggta
     4141 gcgacatggc gaaaagcaga tgggctagat agatattgac atcctcatca aagaaaccgg
     4201 ctcctgaaag gcccggcctg ctgtccctca cccttaaaat ggcttcgaga aaataataga
     4261 tggcagattc ccgttcttca ctgtttaact gaaaaaaaag aaagttttgc cccggttttt
     4321 tttgttccat tgatttatct ctcatggcta tctctttata gcatgtttat ctattttgat
     4381 acttttgaat actagcggat actaaaagaa acatagcatg gcaagtttaa tctgtcaaat
     4441 gtgggggaat ttcgattttt actgtaagtg atttaaaaca aagaggttag gaattttatg
     4501 tctgaaaatt tatttatttt gaaagagccg ggcggatgga atcgataaat cggcgagacc
     4561 ggagtacttt gccggttctg taattgagaa attgggttat cagccggcga acctcggtaa
     4621 aggcgtgagc tgaatgcggc tggcggaggc cctccgtcat gtccattcga ctcagtgttc
     4681 taagcagttc caggcccgcg gggctgatgg cgggggcccc ctggtccctg gagcggcatc
     4741 cgtcgcaaag gagcgccccc tgcttgattg aaaaatatcc ccgttcgatc ggtttcgcgt
     4801 tacaggaaac acaactttcc gtgtagggaa ttaatcccat ttcgccaaaa aacttcaccg
     4861 taaaaacggt tgtaagtttt tccggaggaa tcacggacaa gtaatgaaag gcggtaagga
     4921 gcagttccag gatggccgga tgcgggtcct ggatctcggt cagttcgtcg accaattcac
     4981 aaaggaaact tccgtaggcg atggtttcca gattatcgcg gatggaatga tagctttcaa
     5041 gaatggcgga ttcggtgatc agatgcaggt ccgagcgttt tttttcataa aaaacaattt
     5101 ccgcccgcgt aaaaatttca aagcccgcct gccggatctc cccctcccgg tgcacccctt
     5161 tgaccactcc gcggattttt ccggcttccc gggtaaataa agtcacgatc agggaactgg
     5221 accgaaaggg ctggttttta aggacgacgc cttcaacctt gtgaatactc atgaacgatc
     5281 aggtcccgga cgatggggac caaacccgct tcaaaatctg tttttgcttg agaagcattc
     5341 ggtttttcgt ccgtcgggtt ttatcttcat gtcccagcag atgaagaatt ccgtgacaaa
     5401 ggcagtaatc cagctcttct tcaaatgaat ggccgtattc atcggcctgg gcttcaatgg
     5461 tgggaacgca aagggcgatg tccccgagga aatcggagtt atcccccgcg ttctctttcc
     5521 gggcgccacg ctttttcccg gcgatcttcc cttgcccaaa agcgagcacg tcggtcgacc
     5581 aggcgtgttt aaggtacttc cggttgaggg cccgcatttt cgcgtcgggc acaagaagaa
     5641 tgctgaccag tgctttttta aatccgagat cccgcaaaat ccgggcggca acttttttca
     5701 gttttttttc ggaaactccg gggtgcgggc attcgttcag cacctggatt tcaagacctt
     5761 cctcatcatc ccggaatcga ggcatgatct attcgtccgc ccggaatttc gaaaactgat
     5821 ccgcgccaaa aatgtcgggg gccgaaagcg ggcgatccac cgtgggatat ttcatgcgcg
     5881 tgtggaacat ggccgtcatg tttcggacaa agcttgactg aattttatga agatcgcgga
     5941 gcgtcagttc gcattcatcg agctggccat caatgaattt gtcgttgata attttcctca
     6001 ccagttgccg gatcgtttcg ggcgtcaatt cttgaagcga acgggtcgcc gcttcggctg
     6061 aatccgcgag aagggccacg gccgtttccc tggtttgcgg tttgggcccg ggatatcgga
     6121 agtcgtccgc ccgaacccgg tccgacggag ccgccatatc catcgccttt ttgaaaaaat
     6181 agtaaaccac acccgtcccc tggtgttcgc gtatgaaatc cacgactgcg cggcgaagcc
     6241 ggtacttgcg cgcgatgcgg accccgtccc tgacgtggtt aatgatgatt tcgaaactga
     6301 cccggggcgg gaggtcatcg tgctggttca tgtagagata gccttggttt tccgtgaaaa
     6361 attgcggctt ctcgagttta ccgatgtcgt gaaagtaaca gccgaccttg gcgaggaggg
     6421 cgttggcgcc aattcgttca caggcgtttt ccgcgaggga gcttacgacg aggctgtggt
     6481 gatacgttcc cggagcctcc accatcattt ttcgcatcag ggggtgattg agatccgaca
     6541 gttcaagtaa ggtgacgtcg gtcacgacgt caaaaaagtg ttccaggacg gggagcatga
     6601 ggtaactcgt cacggcaatt aaaagcgcgt tcagaaatcc gagaaagccg agctggagcg
     6661 cgtcccgcca cggagtgctt tgcgtcagct ggaatccggc aatcaccaga aagtaggtgc
     6721 aaccgatccc gagcccgatc cggaggaagt ggcttcgctt gcgtacccgg taagcaagaa
     6781 aagccgtggc cagagaggcc atcagggtcg ccagtagcaa atccacgctg aatcctgaaa
     6841 gaaatccgct gatgaccgtc atcatcactc cacccagaat tccgatccgg ctgtgtatca
     6901 gg
//
LOCUS       DAOSIO010000075         6873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_10028_length_6873_cov_4.231300, whole genome shotgun sequence.
ACCESSION   DAOSIO010000075 DAOSIO010000000
VERSION     DAOSIO010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6873
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10028_length_6873_cov_4.231300"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PK590_03955"
     CDS             <1..433
                     /locus_tag="PK590_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23782.1"
                     /translation="LGGLSGLNYWVFHEIQNRLEIRVTGRFTPDLFRTGFQIRHGSFF
                     WKEKVQLVDGHVQVRYDPWTIFSRDGIRIILKSDYADIRLLGNWMKWKGVESARVELL
                     DVDFVLGSHRLTAINEIEVRSPSFQFVIKNVDRSTRVLGSK"
     gene            439..1947
                     /locus_tag="PK590_03960"
     CDS             439..1947
                     /locus_tag="PK590_03960"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOG23783.1"
                     /translation="MRKRVEDLFLGIPILERIHWDPNRLLNKVTSDSRAVQPGDVFVA
                     CSGACMDGHDFIGQAIHGKAAIVVYEREPDVMIPPHVTGIRVKDSRVALALILRRFYD
                     APDRKIKMVGVTGTNGKTTTAYLLYRLLREQVKIAYLGTLFYELPSGRKPALNTTPGP
                     ETLLPMLAGMAAEGVQYCAMECSSHAIHQNRIRGLEFEMGIFTQLTQDHLDYHGSMER
                     YFQTKRQFFVDDPPPRRLLLNKDCPYGRRMIEELPRASTFSVLAPADYYVENLKMSLT
                     GSTFDLCFHRGKLPFQIRMPMRYNVSNVLAVLAAIDLLGQGENTGFRIEDFRGILAGI
                     PPIPGRMERAGSGTDFSVFVDYAHTPDAIEQVLKDAKSANPKRILTVFGCGGDRDKAK
                     RQLMVKNACQYSDVVIMTSDNPRTESPEEILLDMRKGLPRPAPSHLGIYEIIDRAEAI
                     EKAVSLAKAGDAVFILGKGHEDYQILGDVRIPFNDREVVESAIRRRSRVALS"
     gene            1931..3328
                     /gene="murF"
                     /locus_tag="PK590_03965"
     CDS             1931..3328
                     /gene="murF"
                     /locus_tag="PK590_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOG23784.1"
                     /translation="MLPYPDASRALATPVEFPDESCAIRSFSIDTRTLRKGELFIALP
                     GERSDGHDHVIAAFRRGASGALVQKKRQDGVVKALRQEGLTPRNILAVEDPAAAMASL
                     ARHYRDGLSVKTIVITGSVGKTTTKEFLHFLLKQKYPTLATEGNLNNHLGVPIMISRI
                     KSDHAFAVLEAGASHAGEIDYLASMIRPMSAILTPIGPAHLQGFGTLENVYRAKTEVL
                     RYLSTDSPMVVPDHDERLHQLLRDSGKRFIRVGSSARADYRFSDVRVRNGMVRFKVNE
                     TSTFSFPGQASFLALNATLAIALADQLGVSIAEMPPEWTDLELVSGRFCETVLPNGIR
                     VIDDSYNASPLAFATAIEAFRKITANGKKILVFADMLELGADEVCYHREIGEKIALSG
                     IDAAYAFGDRSRNSIDVIRERSREILDARHFSSADDLVSALGKLLRPGDLVLLKGSRG
                     MRVEKVLAGIERIGF"
     gene            3344..4429
                     /gene="mraY"
                     /locus_tag="PK590_03970"
     CDS             3344..4429
                     /gene="mraY"
                     /locus_tag="PK590_03970"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014261315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOG23785.1"
                     /translation="MLYFIHQFSDISVIFNLFRYITFRSAGASITAFFLCLWFGPACI
                     RWLKKLKAVTDQEREYVESIHHLYKEKKNTPMMGGVLIVGSVLASVLLWGNLTNRFVW
                     LILFVMLWFSAVGFVDDWLKMREKNSHGLGSFTKLIGQVLMGLALGIYLYMDPSFSKY
                     LYVPLLKTPVCYLGLLFIPFVILVLAGSSNALNLTDGLDGLAIGCTLFTATTFAVITY
                     VTGHIGFANYLQISYMPQAGELTVFCAALAGASAGFLWFNSYPAEVFMGDTGSLSLGG
                     SIGTIAVLVKKELLLVIVGGVFVWEAVSVILQVVSFKLTKKRIFLMSPFHHHLQRLGW
                     PESKITTRLWIIAFILAIIGLGSLKVR"
     gene            4431..5720
                     /gene="murD"
                     /locus_tag="PK590_03975"
     CDS             4431..5720
                     /gene="murD"
                     /locus_tag="PK590_03975"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOG23786.1"
                     /translation="MRERKRHVGVLGLKETGYLSSLYLAEKGYQVFATDLSSNDAVKK
                     NAENLRLKGIEAECGGHSMERIISQDWFLISPGIPPASELYQKITTTGKPVHSEIEVA
                     HWFSQAKSVIAVTGSCGKTTLATLIATLIEAVGGKAVLCGNIGNPWIGELARIDRDTT
                     VVLEVSSFQLMHCSKFSPEVGILLNVYPNHMDWHHDMAEYANAKLMLFKNMRNSGTMI
                     CRKKDERTFFPDFKTAAKRVYFDEGRDENPNCAALLCVADVLGISEQKTRSVFAGFPG
                     IEHRLERFLTHKGTVFINDSKCTTPASLAWALEKFSDGTVVLVCGGKAKSSDFGTLKD
                     LVAKKVKCAILIGTSRHMIRDSWRGATEILETADFDEACDWVVKKSAPGDVALLSPAC
                     ASFDMFRNYQDRGRIFKEKIHQRLKINGAGICLKCEK"
     gene            5769..>6873
                     /gene="ftsW"
                     /locus_tag="PK590_03980"
     CDS             5769..>6873
                     /gene="ftsW"
                     /locus_tag="PK590_03980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOG23787.1"
                     /translation="MSRESRILFIVTYLLVAIGVVMIYSSSAILAHEKYHNAEYFLCR
                     QIFFVLLGTVGFFVAASLPVKFYKNNARVFMLLAIFLLLAVYLPGIGRSAGGARRWIY
                     LAGIQFQPVEFAKLACCIYLADYLSRKIAVVQKGNIGIFVPPMILTGILCLLTILEPD
                     LGSTALLALVMAILFFLTGIRLRYVVGVAVVFAVALYALILTKPYRMSRMQAYLNPWQ
                     DPQGSGFQIIQSFLAFALGGLKGVGLGESTQKLFYLPSSHNDFILSVIAEEMGLLGVL
                     FVTALYAIFFICCIRMTQRANQPFEKILITALMLVIILQTLIHMMVATGLVPTKGLPL
                     PFVSYGGSSIVLNLITVGVLISVDRRRPSRGRLK"
BASE COUNT         1541 a         1511 c         1902 g         1919 t
ORIGIN      
        1 gttagggggg ctcagcggat taaattactg ggtgtttcac gagattcaga atcggcttga
       61 gattcgggtg actgggagat ttacgcctga tttgtttcga accggatttc aaatcagaca
      121 cggcagtttt ttctggaagg agaaagtcca attggtggac gggcatgttc aggttcgtta
      181 tgatccgtgg acgatttttt ctcgggacgg gattcgaatc attttgaaaa gtgattatgc
      241 ggacatccgg ttattaggta attggatgaa gtggaagggc gtcgaatccg cgcgcgtaga
      301 actgctcgat gtggattttg tcctgggttc tcatcgcttg acggccatca acgaaattga
      361 ggtcagatcg ccgagttttc aatttgtcat aaaaaatgtt gatcgctcaa caagggtttt
      421 gggttcaaaa tgatctttat gcgtaaacga gtcgaagatc tgtttttggg tataccgatc
      481 cttgaaagga tccactggga tccgaatcgt cttctgaata aagtgacatc ggattcccgg
      541 gctgttcaac cgggagatgt ttttgtcgcg tgttccggcg cttgcatgga cggacatgat
      601 tttattggcc aggcaataca cgggaaggcc gctatcgtgg tctacgaacg agagcctgac
      661 gttatgattc cgccacatgt gacgggaatt cgagtgaaag attcgcgggt cgcgcttgcg
      721 ctgattttga gacggttcta tgacgcgccc gatcggaaga tcaaaatggt cggggtaacg
      781 gggacgaacg gaaaaactac gaccgcttac cttttgtacc gcctccttcg agagcaagtc
      841 aaaatcgcgt acctggggac gcttttctac gaacttccgt cggggagaaa accggcttta
      901 aacacaactc ctgggcccga gacacttttg ccgatgcttg ctgggatggc ggcggagggg
      961 gttcaatatt gcgccatgga gtgttcgtcc cacgcgatcc accagaatcg gattcgtggt
     1021 ctcgagtttg aaatgggaat ttttacgcaa ctgactcagg accatcttga ttatcacgga
     1081 agtatggagc gttactttca aaccaagcgc cagttttttg tggatgaccc gccgccccgc
     1141 cgtttattgc taaacaagga ttgcccgtat ggccggagga tgattgagga gcttccccgt
     1201 gccagtacgt ttagcgttct cgctccggcc gattattatg tggaaaattt gaagatgtcg
     1261 ctgacggggt cgacttttga cctttgcttc caccggggaa agctgccttt tcagattcgg
     1321 atgccgatgc gttataacgt ctctaatgtc ttggctgtcc ttgcggcgat cgatctttta
     1381 ggccaggggg agaataccgg attcaggatt gaagattttc gcggtattct ggcgggaata
     1441 ccgcccattc ccggacgcat ggaacgtgca ggatcgggaa cggatttttc agtcttcgtg
     1501 gattacgccc acacgcctga tgctatcgaa caagtcctga aggacgcaaa gagcgcgaat
     1561 cccaaaagaa ttctgaccgt gtttggttgc ggcggcgatc gggacaaggc gaaacgtcag
     1621 ttgatggtga aaaatgcctg ccaatattct gacgtggtta tcatgacatc cgacaatcct
     1681 cgaacggaga gtcccgaaga aattcttctc gacatgcgga aaggtttgcc gcgtccagct
     1741 ccttcccatc tggggattta cgagatcata gatcgcgccg aggcgatcga gaaggcggtg
     1801 tcgttggcaa aagcagggga cgccgttttt attttgggga agggacatga ggattaccag
     1861 attcttggtg atgttcgaat cccctttaat gatcgtgaag tagtcgagtc tgccattcga
     1921 agaagaagcc gtgttgccct atcctgacgc gagccgggcg ctcgctaccc ccgttgaatt
     1981 tcccgacgag tcttgtgcga ttcgaagctt ctcgatcgat acacggacgc ttcgtaaggg
     2041 cgaacttttt attgcgctcc ccggcgagag aagtgatgga catgatcatg tgatcgcggc
     2101 ttttcgccgc ggggcttctg gcgctcttgt ccaaaaaaaa agacaggacg gcgtggtcaa
     2161 agccttgagg caggaggggc tgacgccgcg aaacattttg gctgtcgagg atccggcagc
     2221 ggcgatggca tcgctggccc gtcactatcg cgacgggtta tcggtaaaaa cgattgtcat
     2281 tactggcagt gttgggaaaa cgacgaccaa agagtttctt cattttcttt tgaaacagaa
     2341 atatccaacg ctggccacgg agggtaatct taacaatcat ctgggcgttc caatcatgat
     2401 ctcacggatc aagtcggatc acgcgtttgc cgtgcttgaa gccggggcaa gccatgcggg
     2461 tgaaattgat tatcttgcat cgatgattcg cccaatgtcg gcgattttga cgccgatcgg
     2521 tccagcccat ctgcaaggtt ttggaactct ggaaaacgtt tatcgcgcga aaaccgaggt
     2581 gctacgttat ctttcaactg attctccgat ggttgttccc gaccatgacg aacggttaca
     2641 ccaactcttg cgtgattcgg gaaagcgttt tatccgggtg ggttcctcgg cgcgtgcgga
     2701 ttaccgtttt tcagacgtgc gtgtgcggaa cggtatggtg agattcaaag tgaatgaaac
     2761 ctcaacgttt tcattccccg ggcaggcctc ttttcttgcc cttaacgcga cgctggccat
     2821 cgcgttggca gatcagttgg gtgtgtcgat cgcggaaatg cccccggagt ggactgatct
     2881 cgagcttgtg tccgggagat tttgtgagac cgtgttgcca aatggtatcc gtgtgattga
     2941 cgatagctat aacgccagcc ccctggcgtt cgcgacggcg atcgaagctt tccggaagat
     3001 cactgccaac ggtaaaaaaa ttcttgtttt tgccgatatg ctggagctag gagcggatga
     3061 agtttgctat cacagagaga taggggagaa aatagctttg agcggaattg acgccgctta
     3121 cgcgtttggg gaccgcagtc gaaacagcat tgatgttatt cgcgaaagat cccgggaaat
     3181 ccttgatgcc cgtcatttct cctcagcgga tgatctggtg agcgctctgg gaaaacttct
     3241 gagaccgggt gatctggtgc ttcttaaagg gtctcgaggg atgcgcgtcg aaaaagtact
     3301 cgcgggtatc gaacggattg gtttttaaca aaaaaagaaa aagatgcttt attttatcca
     3361 tcaattttca gacatttcgg tgatttttaa tctcttccgc tacataacat ttcgctcagc
     3421 gggggcgagc attacggctt tttttctctg tctgtggttt ggccctgcct gtattcgttg
     3481 gcttaaaaag ttaaaggccg tgacggatca ggaacgcgag tacgtcgaat caatccatca
     3541 cttgtacaag gagaaaaaaa acactcccat gatgggcggt gttttgattg tggggagcgt
     3601 tctcgcgtcg gtgcttttgt gggggaattt aacgaatcgt ttcgtgtggc tcattctttt
     3661 tgtcatgctg tggttttctg ccgtcggttt tgtcgatgac tggctcaaaa tgcgtgaaaa
     3721 aaattcacat ggccttggtt ccttcaccaa actaattggg caggttttga tggggcttgc
     3781 gttagggatt tatctttata tggaccccag tttcagcaag tatctttacg tgccactttt
     3841 gaaaacgccg gtatgttatc ttggactttt gtttattccg tttgtgattc ttgttttggc
     3901 gggctcttcg aacgcgctta atttgacgga tggcctggat gggctggcga ttggctgtac
     3961 gcttttcacc gcaacgacgt ttgctgtgat tacctatgtg acggggcata ttggatttgc
     4021 gaattatctg caaatttcct acatgccgca ggcgggcgaa cttacggttt tttgtgccgc
     4081 cctggcgggc gcgagcgcgg ggtttctctg gtttaattcc tatccggctg aagtatttat
     4141 gggagatacg ggatcccttt ccttgggagg atccatcggg acgatcgcgg tgctggttaa
     4201 aaaggaactc cttcttgtga tcgtcggcgg tgtttttgtc tgggaggcgg ttagtgttat
     4261 tctccaggtt gtgagcttca agctcacaaa aaagagaatt ttcctcatgt ccccgtttca
     4321 ccatcatctt caacgattgg gatggcctga atccaaaatt acgacccgat tatggatcat
     4381 cgcatttatt ctcgcgatca ttggtctggg ttcgttaaag gttcgctgaa atgcgggaga
     4441 ggaagcgaca cgttggggtt ctcgggctca aagagacagg ctacctcagt tccctctacc
     4501 tcgcagagaa aggctaccag gtttttgcga cggatctctc atcgaatgat gctgtcaaaa
     4561 aaaacgcgga gaatcttcgg ttgaaaggga ttgaagccga atgcggcggg cattccatgg
     4621 aacgaataat atctcaggac tggttcctga tatcgccggg tatccctccg gcctccgaac
     4681 tttatcaaaa aataaccact acaggaaaac cggtgcacag tgaaattgag gtcgcacact
     4741 ggttttctca agcaaaatca gtgattgccg tgacgggttc gtgcggaaaa acgactctgg
     4801 cgacgttgat tgccacgctc attgaggccg tgggaggaaa ggcggttctt tgtgggaaca
     4861 tcgggaatcc ctggatcggt gaactggccc ggatcgatcg cgatacaacc gtggtcttgg
     4921 aagtcagctc cttccaatta atgcactgtt caaagttttc acccgaagtc gggattttgc
     4981 tgaatgttta tcccaatcat atggactggc atcacgacat ggcggaatac gcgaatgcca
     5041 agcttatgct ttttaaaaac atgagaaact ccgggacgat gatctgccga aaaaaagacg
     5101 aaagaacatt cttcccggat ttcaagacgg ccgcaaagcg ggtttacttt gatgaaggcc
     5161 gagatgaaaa tcccaactgc gccgcgttgc tttgtgttgc cgatgtcctg ggcatttctg
     5221 aacaaaaaac gcggtcggtt tttgccggtt tccccggaat tgagcaccgg ttagaaagat
     5281 ttttgactca taagggaacg gtttttatca atgattcaaa atgcacgacg ccggcctctc
     5341 tggcttgggc gcttgagaag ttcagtgacg gcacagttgt tcttgtctgc ggagggaagg
     5401 ccaaaagcag tgattttgga acattgaaag acctggtcgc gaagaaggta aaatgcgcga
     5461 ttcttatcgg gacctcacga cacatgattc gtgactcttg gcgcggcgcg accgagatac
     5521 ttgaaaccgc cgattttgat gaggcctgtg attgggtcgt caaaaaatcc gctcccgggg
     5581 atgttgccct cttgtctccg gcctgcgcga gttttgacat gtttagaaat taccaggatc
     5641 ggggaagaat ttttaaggaa aaaatccatc agaggttgaa aataaacggg gcgggaattt
     5701 gcttaaaatg cgaaaaataa ttatgatgcc gcctggtttt tttgtcattg tttttgaggg
     5761 agaaggacat gtctagagag tcgcggattc tttttatcgt gacgtacctt ttagtggcca
     5821 ttggggttgt catgatttac agctccagcg ccatattggc acatgaaaaa tatcacaacg
     5881 ccgaatactt tttatgcagg cagatttttt ttgtgttgct tgggacggtt ggattttttg
     5941 tcgcggcgtc cctgccagtt aaattttaca aaaataatgc ccgggttttt atgctactgg
     6001 ctattttttt gcttttggct gtttatcttc ccgggatcgg acggtcggcg ggtggtgccc
     6061 gtcgatggat ttaccttgcg ggtatccagt tccagccggt ggagtttgcc aagttagcct
     6121 gctgtattta tctcgcagat tacctgtccc ggaaaatagc cgtagtccag aaggggaata
     6181 tcggcatctt tgtgccgccg atgattttga cgggcatttt gtgtctcttg acaattcttg
     6241 aaccggatct tggttccacg gcactgctcg cgcttgttat ggcgatcctc ttttttttga
     6301 cgggaatccg gttgcgttat gttgtcggtg tggcggtcgt tttcgcggtc gcgctttacg
     6361 cactgatttt gacgaagccg tatcgaatga gccggatgca ggcttacctc aatccgtggc
     6421 aggatcctca ggggtcgggg tttcaaatta ttcagtcttt tcttgctttt gccctcggag
     6481 gacttaaggg ggtggggctt ggagaaagca cgcaaaaact tttttatctt ccctcgagcc
     6541 ataatgattt catcttgtcg gtgatcgcgg aagaaatggg gcttttggga gttcttttcg
     6601 tgaccgcgct ttatgccatt ttttttatct gttgtattcg catgacacag cgcgcgaatc
     6661 agccgtttga aaaaattctg atcacggcgt taatgttggt tattatcttg cagacgctca
     6721 ttcatatgat ggtcgcgact gggctcgtgc cgacgaaagg tcttccgctt ccgtttgtaa
     6781 gttacggggg aagctctatt gttttgaatc tgattacggt gggcgtcctg atttctgtgg
     6841 atcgccgccg accgtctcgt ggtcgattga aat
//
LOCUS       DAOSIO010000076         6815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_10134_length_6815_cov_3.198077, whole genome shotgun sequence.
ACCESSION   DAOSIO010000076 DAOSIO010000000
VERSION     DAOSIO010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6815
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10134_length_6815_cov_3.198077"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1503
                     /locus_tag="PK590_03985"
     CDS             <1..1503
                     /locus_tag="PK590_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG23788.1"
                     /translation="GGKTLSSLAFALRHANICNEQKDKEKSHTSPGVRRIVYVIPFTS
                     IIEQNAEEFRKAIASIEGGAANQLVIEHHSNFDPDKETPYSRLACENWDAPLIVTTSV
                     QFYESLFANRTSNCRKLHNLSNAIIIIDEAQTIPVDYLEPCLRALKELVLNYGATVVL
                     CTATQPAVHLRPDFRIGIDHVREIIDDPPALYRRLKRVEVNHLGTVSDTELVKMLRAH
                     EQALFIVNTVRHAQTLSRLLGEDPSHFHLTAAKCPAHRSVELKVIRERLDQGKPCRVV
                     STQLVEAGVDVDFPVVYRSMAGIDSIAQAAGRCNRHGRMSSLGKVYVFQSEHGSSERF
                     VSDTTKCAGEIFAMYDNDPMSLEAINHYFKLYYWDQDSRWDTKHICDSFKLVNDRDLP
                     FLFDFSRVAGDFKLIQQMGRRPVVVPWGEEGRLLCEKLKVIPSLNREVCRYLQRYTVQ
                     AQKRIWLSHLDRGIKPLFDGSLGVLTCPEIYYSKNYGLSFEEPSGEGFIV"
     gene            1519..2250
                     /gene="cas5c"
                     /locus_tag="PK590_03990"
     CDS             1519..2250
                     /gene="cas5c"
                     /locus_tag="PK590_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HOG23789.1"
                     /translation="MAYGIKIKVWGDYASFNRPEMKVERVSYDVMTPSAARGVLEAIH
                     WKPGMRWVVDEIHVLKPIQFTHVRRNEISCKIPTGEIARAMRGESVDLGIAVDDPDVR
                     QQRAAMILRDVCYGIAAHIDILRPDLGPDGKPLANPEAKHLEAFKRRAEKGQFFHQPY
                     LGTREFPASFELVDEFPSCPKELMGERDLGLMLHDMVFTEDQDNGKIIESSQWKRVSA
                     EARFFKVIMTDGVIRVPALAERRPS"
     gene            2247..4034
                     /gene="cas8c"
                     /locus_tag="PK590_03995"
     CDS             2247..4034
                     /gene="cas8c"
                     /locus_tag="PK590_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HOG23790.1"
                     /translation="MILQSLYELYDRLSGLADNPYEISPFGYSLQKIAFRLVITNDGR
                     LHELESLRDPKTNLPKQMIVPGGDKPTGKVTERSAHKKTQFLRNNLSFLLGISVEGDK
                     NPALALAQMEFEAFKKVHLEREQQINDPDYSVFCKFLRHWKPEAGLAHGDWIAFGDGQ
                     GVIKLIGKTEYLHDRPAVRAWWDENQPKNKSKPVQCLITGDLKPASRLHEPKIRSVKD
                     SQPAGAPIVSFDKGSDAFSSYGHDGEQGLNAPVSEEATFRYATALNSLLAGPQSWKHR
                     FTLGDTTVVFWTDKPSDAEDIFAQFAKEGSTVPKKEEVQDEALLQKMQIFLKVLREGR
                     QAYTEIDKNPDQTNFFILGMTGQARGRIGVRFFYKDTVGHLLDNLRKHYNDMKIIRQY
                     EEGAKYPDSEFPPTWLLLRQTARDKDDIPPILSGPLLRAVITGSLYPEGLYKAVIRRV
                     HADREINYLRTSVIKGYLVRNQKQEVSMSLDPGRKDPAYRIGRLFSALEKTQTDALGE
                     VGSSIKDRFYSAASAMPRSVFPRLLRLYSHHLGKLSVGMRVNREKLVQEIMCEIHEFP
                     GHMNLSDQGLFAIGYYHQMCDFYRGKKVE"
     gene            4089..4961
                     /gene="cas7c"
                     /locus_tag="PK590_04000"
     CDS             4089..4961
                     /gene="cas7c"
                     /locus_tag="PK590_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HOG23791.1"
                     /translation="MKRYDFVYLFDVKDANPNGDPDAGNLPRVDPETGHGLVTDVCMK
                     RKIRNYVGIKGGEKNRIYYTEGSVLNKTSKEAWESVGLKPDKKLPQDAQKAKAITQYM
                     CDHFYDIRAFGAVMSNEVNCGQVRGPVQISFARSVEPIISSEHTITVCAARKEDKPVE
                     EQVGIQGRKFTVPYGLYRAQGVVNPLLAEKTGFSEDDLKLLWEALAKAFEIDQSAARP
                     AGSMSARMLIAFKHDSELGNAPAHKLFDLVRITPVGESGKPARSFSDYEKGISVPPEG
                     KLAEFPGVEVIHIF"
     gene            5274..5927
                     /gene="cas4"
                     /locus_tag="PK590_04005"
     CDS             5274..5927
                     /gene="cas4"
                     /locus_tag="PK590_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020586893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOG23792.1"
                     /translation="MKKQMFLEEDFIQLSALQHFVFCPRQCALIHIEQMWGENLLTAE
                     GRIMHERAHAETFEIREGIRIERGMPLRSDNLGLNGKADVIEFHKAADGKSWIPFPVE
                     YKRGKPKADNCDKVQLCAQALCLEEMMKTEIRTGAIFYGRTKRRQEVIFDPALREETK
                     KAVFEVHEFIKTGKTPKPFYAKKCEACSLVEVCMPKTMGCKRSVREYCDNAMREENL"
     gene            5924..>6815
                     /gene="cas1c"
                     /locus_tag="PK590_04010"
     CDS             5924..>6815
                     /gene="cas1c"
                     /locus_tag="PK590_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HOG23793.1"
                     /translation="MKKLMNTLFVTATGAYLSKEGETVVVSVEKETKLRVPIHTLGGI
                     VCFGHVSMSPYLMGFCAENDVGVSFLTEYGRFLASVRGKVSGNILLRREQYRKADDLE
                     FCREIAKVMIKGKLSNCRTVLQRALRDHNDKINSQEVGAASQKMLFSLRKLDSALTLD
                     EVRGIEGDSAHVYFNVFDHLIISQKENFRFEDRNRRPPLDNVNCLLSFLYTLLMHDMR
                     SALECVGLDPYAGFLHRDRPGRASLALDMMEEIRPFLADRLALSLINLNQVTAKGFKK
                     GETGAVLMDDETRKTLLVAYQ"
BASE COUNT         1824 a         1368 c         1858 g         1765 t
ORIGIN      
        1 ggcggcaaaa cgctctcgtc tttggcattt gctcttcgac acgccaatat ttgtaatgag
       61 cagaaagata aagaaaaatc gcatacctcc cccggcgtaa ggcggattgt ttacgtaatt
      121 ccttttacga gcattatcga acaaaatgct gaagaattcc ggaaagcaat agcgtctatt
      181 gaaggggggg ccgccaacca acttgttatc gaacatcaca gcaattttga tccggataag
      241 gaaacgcctt actcccgctt ggcctgcgag aattgggacg cgccccttat tgtgacgaca
      301 tcggtgcagt tttatgaatc gctctttgca aacaggacgt caaattgccg caaactgcac
      361 aatctttcaa acgcgattat tattattgat gaagcgcaga ctattccggt tgactatctt
      421 gagccatgtt taagggcatt gaaagaactg gttctgaatt atggcgcgac ggtggttctt
      481 tgcacggcga cacagcccgc ggttcatctg aggccagact tcaggatcgg gattgatcat
      541 gtgcgtgaaa taatcgatga tccccccgca ttgtacaggc gactgaaacg agtggaagtg
      601 aatcatcttg gaactgttag cgatacagag ctggtcaaga tgctgagagc ccatgaacag
      661 gcgctcttta ttgtgaacac cgttcgacat gcccaaacgc tctcgcgatt attgggagaa
      721 gatccctcgc atttccattt aactgcggcc aaatgccccg cccacaggtc ggttgaattg
      781 aaggtgattc gagagcgact ggatcaggga aagccctgcc gtgttgttag cacacagctt
      841 gtcgaggccg gtgtggatgt ggatttcccc gtagtttaca gatcgatggc aggaattgat
      901 tccatcgctc aggccgccgg gcggtgcaat cgtcacggga ggatgtcatc tttggggaaa
      961 gtttatgttt ttcaatctga acatggttcc tcagaaagat ttgtctcgga taccacaaaa
     1021 tgtgcagggg aaatattcgc aatgtatgat aatgatccaa tgtcgcttga agcaataaac
     1081 cattatttca aactctacta ttgggatcag gacagccgct gggacacgaa gcacatttgc
     1141 gattctttta aattggtaaa tgatagagac cttccgtttt tatttgattt ttcacgtgtt
     1201 gctggtgatt tcaagctgat tcagcaaatg gggcggcggc cggtggttgt cccgtggggt
     1261 gaagaaggtc gcctgttatg cgaaaaacta aaggtaattc catctctcaa cagagaagtc
     1321 tgccgctatt tgcaacgcta tacggtacag gcgcagaaac ggatttggtt gagtcacttg
     1381 gatcgtggca tcaaaccgct ttttgacggg agtttgggcg ttttaacttg ccctgaaatt
     1441 tattattcca aaaactatgg cctgagtttt gaagaaccat cgggtgaagg atttatcgtc
     1501 tagaaaggag aaaataaaat ggcttacgga attaaaataa aagtttgggg ggattacgcg
     1561 tcttttaatc gtcccgaaat gaaagttgaa cgggtttcct acgatgtcat gacaccgagc
     1621 gcggcaagag gcgttctgga ggcgattcac tggaagcccg gcatgcgttg ggttgtcgat
     1681 gaaattcatg ttttgaagcc cattcagttt acgcatgtcc gccgcaatga aatcagctgt
     1741 aagattccta cgggggaaat tgcccgggcc atgaggggag aatcggtgga tttgggaatt
     1801 gcggtcgatg atcccgatgt acgacaacag cgggctgcca tgattttaag ggatgtctgt
     1861 tacgggattg ctgcgcacat tgatatttta aggccggatc tggggccgga tggcaagccg
     1921 cttgccaacc ctgaagcaaa gcatcttgag gcgttcaaac ggcgggcgga aaaaggccag
     1981 ttttttcatc agccctattt ggggacaagg gaattccccg cgtcttttga gcttgtagat
     2041 gagtttccgt cctgccctaa agaattaatg ggcgagcggg atttggggct tatgcttcac
     2101 gatatggttt ttacggagga ccaagacaac ggaaagatca ttgaatccag ccaatggaag
     2161 cgggtcagtg cggaagcgcg gttttttaaa gttatcatga cagacggtgt tattcgcgtg
     2221 cctgcgttgg cggaaaggag gccgtcatga tccttcaatc gctctatgaa ctgtatgatc
     2281 ggttaagcgg tttggctgac aacccttatg aaatttcccc atttggatat agtttgcaga
     2341 aaatagcttt ccgtttggtg attaccaatg atgggagatt gcacgaacta gagagtttgc
     2401 gtgatcccaa aacgaacctt cccaagcaaa tgattgttcc agggggtgat aaacctaccg
     2461 gaaaagtgac tgaacgatct gctcataaaa aaactcagtt tttaaggaat aatttgtcgt
     2521 ttctgcttgg gatttcggtt gagggtgata aaaatccggc gcttgcatta gcgcaaatgg
     2581 aatttgaagc attcaaaaag gttcaccttg aaagagagca acagattaac gatccggatt
     2641 attcggtgtt ttgcaagttt ttaaggcact ggaagccgga ggccggatta gctcatggag
     2701 attggatagc tttcggcgat gggcagggcg tgatcaagct gattggaaaa acggagtatt
     2761 tgcatgatag acccgccgtg cgtgcctggt gggatgaaaa tcagcctaag aataaaagta
     2821 agcccgtgca atgtctgatt accggagatt taaaaccggc atccaggttg catgaaccca
     2881 aaataagaag cgtaaaagat tctcagcctg cgggagcgcc tattgtctcg tttgacaagg
     2941 ggagcgatgc tttttcttct tatgggcacg atggtgaaca ggggctgaac gcacccgtgt
     3001 ctgaagaggc tactttccgg tacgcaacag cactcaactc cctgttggct gggccccaaa
     3061 gctggaagca tcgttttaca cttggcgata cgactgtggt tttttggacc gataaaccgt
     3121 ctgatgcgga agatattttc gcgcagtttg caaaggaggg ttcaaccgtt cccaaaaaag
     3181 aagaagttca ggacgaagcg ctactacaga aaatgcagat tttccttaaa gttcttcggg
     3241 agggccgtca ggcttatacg gagattgaca aaaaccccga tcaaaccaat tttttcattt
     3301 tgggaatgac gggtcaggca agaggacgga tcggtgtccg ttttttctac aaggataccg
     3361 ttgggcacct tctggataat ttgcggaaac attataacga tatgaaaatt atccggcagt
     3421 atgaagaggg agcaaagtat ccggattcgg agttcccgcc aacctggttg cttttacgcc
     3481 aaacagcccg ggataaagac gatatccctc cgattttatc ggggccgtta ttgcgtgcgg
     3541 tgattaccgg ttccctctac ccggaaggtt tgtacaaagc ggtgatccgg cgcgttcatg
     3601 ccgatcggga aataaattat ttaagaacga gcgttattaa ggggtattta gtcagaaacc
     3661 aaaaacagga ggtgagtatg agtttagatc caggacgaaa agatccggct tatcggatcg
     3721 ggcgtctttt ttctgcgctt gagaagacgc agacagacgc tttaggtgaa gttggttcat
     3781 ccattaaaga taggttttac agtgcggcct cggcgatgcc ccgaagtgtt ttcccaagat
     3841 tgttaaggtt gtattcgcac cacttgggga agctatccgt gggtatgcgt gtcaatcgcg
     3901 agaaactggt gcaggaaatc atgtgcgaaa ttcatgagtt cccggggcac atgaatctct
     3961 cggatcaggg gttgtttgca attggctatt atcaccagat gtgcgatttc taccgtggga
     4021 aaaaggtgga gtgaagtgtt tagaaacagt ttgtcgattg tcaacgccga aaacagaaag
     4081 gataaatcat gaagcgctat gactttgtgt acctgtttga tgtgaaagat gctaatccga
     4141 acggtgatcc ggatgcagga aacctgcctc gggtcgatcc cgaaaccggg catggtttgg
     4201 taacggatgt gtgcatgaag agaaaaataa ggaattatgt tgggattaaa ggtggtgaga
     4261 aaaaccggat atactatacg gagggctctg ttttaaacaa gacgagcaaa gaggcatggg
     4321 agtctgttgg cttgaaaccg gacaaaaaac ttccccaaga tgcccagaaa gcaaaagcta
     4381 ttacgcagta tatgtgcgat catttttatg acatcagagc atttggagcg gtaatgagca
     4441 atgaagtcaa ttgcggacag gttcgtggcc cggttcaaat ctctttcgcg cgttcagtgg
     4501 agccgatcat ttcttcggaa cacactatta cggtttgtgc ggctcgcaaa gaagataagc
     4561 cggttgagga acaggttggt attcagggac gaaaatttac ggtgccgtac ggtttgtatc
     4621 gcgcgcaggg tgtcgtgaat ccgttgttgg ccgaaaaaac cggattttcc gaagatgatc
     4681 tcaaattact ttgggaagcg ctggcgaagg cttttgaaat tgaccagtct gcggctcgcc
     4741 ctgccggcag tatgtctgcc cgcatgctga tagcattcaa gcacgattcg gagttgggaa
     4801 atgctccggc gcacaagttg tttgatttgg tcaggatcac gcctgtaggg gaatcaggaa
     4861 aaccggcgcg gtcgttcagt gattatgaaa aaggtatttc ggtccctccg gaagggaaac
     4921 tggcggaatt tccgggtgtt gaggtcatcc atatcttttg attcgggaca gcagatgcaa
     4981 ggtggttgcc ttttgtaacc acttgggccc cgggcagttt tccctggggc gcttacccaa
     5041 gaggtatccc gccttgggcg ggataccgtg gggttgcctt gaaaatcccg ggttcgcgac
     5101 caagcggacg cgaattcttt acaagttgga agggtggaac gcgggaagaa aaagcagatg
     5161 ttgatgccgg agggaaatta gccggtaatg cggggaaaac tttaggaaaa tggctcagaa
     5221 gattcatggt ctctgcgaaa aattatctga aggtgtctga ggcgcaaaag aggatgaaaa
     5281 agcaaatgtt tctggaagag gattttatcc agctttcagc gcttcagcat tttgtgtttt
     5341 gtccccggca gtgcgcattg attcatattg agcaaatgtg gggagaaaac ctgttgacgg
     5401 ccgaaggtag gattatgcat gaacgagccc atgccgagac ttttgaaata cgggagggta
     5461 tccgtattga acggggaatg ccgctgcgtt cagataatct tgggttgaat ggtaaggcgg
     5521 atgttattga gtttcataag gccgctgacg gcaagtcatg gatccccttc ccggtcgaat
     5581 ataaaagggg aaaaccaaaa gcggataatt gtgacaaggt tcagctttgc gcgcaggctt
     5641 tgtgccttga agagatgatg aaaacagaaa ttcggaccgg cgctattttt tacggcagga
     5701 caaaaagacg acaggaggtc atttttgatc cggcgcttcg cgaggaaaca aaaaaagccg
     5761 tttttgaagt acatgagttt attaaaacag ggaaaacacc gaagcctttt tatgcgaaaa
     5821 aatgcgaagc ctgctctctg gtcgaggtct gtatgccgaa aaccatggga tgtaaaaggt
     5881 cagttcggga atattgcgac aatgcaatga gagaagaaaa tttgtgaaaa aattaatgaa
     5941 tacgcttttt gtgacggcaa caggtgctta cctttctaaa gaaggtgaaa ccgtggtggt
     6001 ttccgttgaa aaggaaacca aactgcgggt tcccatacac acacttggcg ggattgtttg
     6061 tttcggacat gtcagcatga gtccctactt gatgggattt tgtgccgaaa acgatgtggg
     6121 tgtgagcttt ttaacagaat acggacgttt tttggcgagt gtccgcggta aggttagcgg
     6181 caatatcttg cttcgtcggg aacaataccg aaaagctgat gatctggaat tctgccgtga
     6241 aatagcgaaa gtgatgatca agggcaagtt gtcaaattgc cgcaccgtct tgcaaagagc
     6301 gctccgggat cataatgata aaataaatag tcaggaagtt ggcgctgcat cccagaagat
     6361 gttgttttct ttgcgtaaac ttgacagcgc tctcacttta gatgaagtga gaggaatcga
     6421 aggagattcc gcccatgttt attttaacgt ttttgatcac cttattatct ctcagaaaga
     6481 aaatttccgg tttgaggacc gcaaccgcag gcccccgctg gacaacgtca attgcctgct
     6541 gtcttttctc tatacacttt taatgcatga tatgcgttct gcgttggaat gcgtcggcct
     6601 tgatccctat gccggatttt tacatcgtga taggcccggc agagcaagtt tggcgcttga
     6661 tatgatggaa gaaatcaggc cttttttggc cgaccgtttg gcgttatccc taatcaatct
     6721 taatcaagta accgcaaagg ggtttaaaaa gggcgaaaca ggcgctgtct tgatggacga
     6781 tgaaacaaga aaaactttgt tagtcgcata tcagg
//
LOCUS       DAOSIO010000077         6775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_10203_length_6775_cov_4.152679, whole genome shotgun sequence.
ACCESSION   DAOSIO010000077 DAOSIO010000000
VERSION     DAOSIO010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6775
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10203_length_6775_cov_4.152679"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2538
                     /gene="ileS"
                     /locus_tag="PK590_04015"
     CDS             <1..2538
                     /gene="ileS"
                     /locus_tag="PK590_04015"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOG23794.1"
                     /translation="EHACLKEMGKRKEDVDRVAFRKQARQYAERFVGIQREEFKRLGI
                     FAEWEKPYLTMNYEYQTAIAESFLKLYSEGYIEQRLKPVPWCSDCETALADAELEYED
                     KVSKSVYVKFKLIKETLNGKVSPVEIHKFGERPVYVLVWTTTPWTLPANVGIALHPEL
                     SYSFLDMGEEVWLLADARIPFIRSLGLKSPLAEPIVSVQGSVLNHAEYEHPFLPRKGC
                     AILADYVSSEDGTGIVHIAPGHGEEDYKFGHLENNLAIFSPVDSKGRFTDEFPYCKGE
                     HVFKANAKITDLLREKKLLLHEADHAHSYPHCWRCKKPILFRATTQWFMKIDHKDLRQ
                     RMLNAIHQDIRFVPDWGKNRIGSMVQTRPEWCLSRQRYWGVPIPIIKCAKCAGIHLVK
                     ESRQKILEFFRNEGADSWFAHPAEDFLPEGTKCPKCGGAEFEKETDIIDVWFDSGVSH
                     QAVLRQYPGLQFPADLYLEGSDQHRGWFQSALTTSVALEGKSSFKGVLTHGFVVDGQG
                     KKMSKSAGNVVTPQEVMKEFGADVLRLWVASCDYQFDIRLSNEILKQLVDAYRKIRNT
                     YRYLLSNLYDFDVTKDALPFEKQHPVDQWVIGKADQLVETVTDLYERFEFHQIYREIY
                     NFCVIDLSAYYLDILKDTLYTAAPSAWLRRSAQTSLHYVLSRLVKMTAPIMPFTSDEV
                     WNAYKLEPGVPSVHASSWPVPKGQPVHQNPGYKDWEHIREIRDAVTPYLEKQREAKTI
                     GAGLEAKVLMTVDLPESADILQRNEKELARVFVVSQVSLDEAALAGVEATEIFSAVLQ
                     TKLKLKVIVAQADGLKCVRCWNYSTAVGQHPEHPQLCPKCIEALQSR"
     gene            2568..4124
                     /locus_tag="PK590_04020"
     CDS             2568..4124
                     /locus_tag="PK590_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG23795.1"
                     /translation="MMFFHLRNPKTVLRKILVGLILFFIGAMAAYLVSLSLPHVVGNP
                     WGAHPLTPILTMTLIFLVAYKPVERCVKVFLEKNIFRKKSYAQWTLMELAEELRTNLD
                     FQELSNLIVNTFGEALHLKTVALLIRDVTRDGFEIASAFGWPLATSRRIFLGNDSPLV
                     RCIHRMGPHVLVRGPLLKSLSWQEANEMARDFDLVRSCWVLPLFSKGDLIAMIAFGAH
                     SPDTVFDEWDFHFFRKFGESVAPCVANSRYVQGLRRANFELQDSQAEWFQKTKMSAIE
                     KLAAGIAHEVHNPLAIISGKAQVLLMQKKRTPLEPHVEEALNVIVKQTRRAADITRKL
                     LMYSQDSRAPQEEISFEKILDETIALVGYQASLDKIEIIKKTDPSLPTFWGNTQEIRE
                     IFLNLFLNAVEAIGDQGGRIQVEVRISEGEDVIEIRFQNSGKEIPQEDLDRIFNPFFT
                     TRHEAVGLGLFVTRQIVKRYGGAIRVESQSGEGSLFIIQLPRTTAEGETPRASAEEPK
                     EGTILVNKGA"
     gene            4156..4530
                     /locus_tag="PK590_04025"
     CDS             4156..4530
                     /locus_tag="PK590_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG23796.1"
                     /translation="MKKKLLLVDDEVEICEFLKSFFEDRDYQVATAHSGVQAVEQVES
                     FRPDVVLLDIQMPEMDGLSALREIKEKYPRVKVIMVTAVETQDRIEAAMRLGADNYIT
                     KPLSLEYLEKDVQEKIESLAGI"
     gene            4543..5817
                     /locus_tag="PK590_04030"
     CDS             4543..5817
                     /locus_tag="PK590_04030"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOG23797.1"
                     /translation="MTNLTRTNRQKKTEQKFDYQEALRDVARSMVRLKRPDRLLKLIT
                     RFIDRQFGLHHTSLLVHEPSKNRYVFVNSKGARRVPAGLLKFDSDHPLIRWFALPRRK
                     TPFQEDYLLLTDIKKTLNKAHFRLAADSRASELVKVQKTMEVLRVELVIPGYFKNDLV
                     GILMLGGKFSGRFFTSSEILFFQTLAHDCSMAIKTAEYNQSLLEKNRELENRLQEIEK
                     LRHKEQRTYYEIIRSLAEEVEAKEPTVFGHVSQVEKLGMMTARELGIDLSGQHKDILS
                     AALMLHDVGKIGVPDHILMKPGSLTPEEWEVMKSHVEKGAKILEPLSDFRKVKEIILS
                     HHERFDGTGYPRGLKGEDILIEARIVSVVDSFHAIVSNRCYREGRSIDFALQELEHCA
                     GTQFDPQVVEAFIRVIRREIAKGRIDVAALQH"
     gene            5895..6482
                     /locus_tag="PK590_04035"
     CDS             5895..6482
                     /locus_tag="PK590_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HOG23798.1"
                     /translation="MPKKKKVKKKAKKTKARRVVKPKAKVAKGKKGSKRHKAPRESKV
                     VPIMGYRVDRIVKVPKGPQLKKTPFSKSELGVFTKLLMDLKRRIVGSLQSIEKENLNT
                     GRKDSTGGFSGYSFHVTDAASDSFDRELNIGLAANSQQLLNDIAVALRKIEEGTFGIC
                     EKYGIAIPKKRLLAAPYVRLCLQAQEEEEREPRRS"
     gene            6479..>6775
                     /locus_tag="PK590_04040"
     CDS             6479..>6775
                     /locus_tag="PK590_04040"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOG23799.1"
                     /translation="MIFFYGLVGFVVFLDQLTKYFALVFLSEVSQPVLPNVFHLTLVR
                     NTGVAFGFLSDHSSLLFSVITVSLVFLFILVARSHGASLSEKWALSLILGGAIGN"
BASE COUNT         1672 a         1519 c         1858 g         1726 t
ORIGIN      
        1 gagcacgcgt gcctgaagga aatggggaag cgcaaggaag acgtggaccg ggttgccttc
       61 cggaaacagg ctcgtcaata tgccgaaaga tttgttggga tccaacgtga ggaatttaag
      121 cgactgggaa tatttgccga atgggaaaaa ccctacttga cgatgaatta tgaatatcag
      181 accgcgatcg cggagtcttt tctcaaatta tacagcgaag gatatatcga acagcggtta
      241 aagcccgttc cctggtgctc tgattgcgag accgcgctgg ctgatgccga gcttgagtac
      301 gaggataagg tttccaaatc ggtttatgtg aagtttaagc ttatcaagga aaccttgaat
      361 gggaaggtta gtcccgtaga aatacacaag ttcggagaac ggccggttta cgtgcttgtt
      421 tggacgacga cgccgtggac gcttcccgcc aacgtcggga tcgcgctcca tccggagctt
      481 tcttactctt ttctcgacat gggggaagaa gtatggctcc ttgcggacgc gcgtattcct
      541 tttattcgct ccctcggcct caagtcgccg ctcgcggaac cgatcgtcag tgttcagggc
      601 tctgttctca atcacgcgga gtatgaacac ccgtttcttc cccgaaaggg atgcgctatt
      661 cttgcggact atgtttcctc tgaagatggc acggggattg tccatattgc tccgggacat
      721 ggtgaagaag attataaatt cggtcatctt gaaaataatc tggccatttt ttcgccggta
      781 gattcaaaag gccggtttac ggatgaattc ccttattgca agggtgagca tgtcttcaag
      841 gcaaatgcga agattacgga tcttctcagg gaaaagaagc ttttacttca cgaagcggat
      901 cacgcgcatt cctacccgca ttgctggcgc tgtaaaaagc cgatcctttt tcgcgccacg
      961 acccagtggt tcatgaagat tgatcataag gatttgcgtc agaggatgct gaacgcgatc
     1021 catcaggaca tccgctttgt gcccgactgg ggtaaaaacc ggattggttc catggttcaa
     1081 acgcgtcccg aatggtgtct ttcccgccag cggtactggg gagttcccat tccgattata
     1141 aaatgcgcga agtgcgcggg gatccacctg gtcaaggaat ccagacagaa gattcttgaa
     1201 ttctttcgca atgaaggcgc ggattcctgg ttcgctcatc cggctgaaga ttttcttccc
     1261 gaggggacaa aatgcccgaa atgtggcgga gccgaatttg aaaaagaaac ggatatcatt
     1321 gatgtctggt tcgattccgg ggtcagtcac caggccgtcc ttcgacaata cccggggctt
     1381 cagtttccgg cggatcttta tcttgaaggt tcggatcagc accgggggtg gttccaaagc
     1441 gccctgacca cttcggtggc gcttgagggg aaaagttcgt tcaagggtgt cctcacgcat
     1501 ggttttgtgg tggatggaca ggggaaaaag atgtcgaaat ctgccggaaa cgtggtgact
     1561 ccccaggaag tgatgaaaga gtttggcgcg gatgtcttga ggctttgggt ggcatcttgc
     1621 gattaccagt ttgacatccg tctttcgaat gaaattttga aacagcttgt ggacgcgtat
     1681 cgcaagatcc ggaacaccta ccgctatttg ctttccaatc tttacgattt tgacgtcaca
     1741 aaagacgcgt tgccttttga aaaacaacat cccgtcgatc aatgggtgat cggaaaggcc
     1801 gaccagttgg tcgaaacggt gacggatctt tacgagcgtt ttgagtttca ccagatttat
     1861 cgtgagattt ataatttttg tgtgatcgat ctgagcgcgt attatctcga tattctcaaa
     1921 gacaccttgt ataccgcggc gcccagtgct tggctccgcc gttcggcgca gacatccctg
     1981 cattatgtgc tttcgaggct ggtcaaaatg acggcgccca tcatgccatt tacgtctgat
     2041 gaggtgtgga acgcttataa gcttgaaccg ggggtaccga gcgtgcacgc gagttcatgg
     2101 cctgttccga aggggcaacc ggttcatcaa aatcccggat ataaggattg ggaacatatt
     2161 cgcgaaattc gcgacgcggt gactccttat cttgaaaaac agcgtgaagc aaaaacgatc
     2221 ggggcgggtc ttgaggcgaa agtgctaatg actgtagact tgccggaatc tgccgacata
     2281 ttacagcgga atgaaaagga gctcgcccgg gtttttgtcg tatcccaggt ttctctggat
     2341 gaggcggctc tcgcgggcgt ggaagccaca gagatttttt ccgcggtgtt gcagacaaaa
     2401 ctgaaactca aggtgattgt ggctcaagcg gacgggctca agtgtgtgcg ttgttggaat
     2461 tattccaccg cggtgggcca gcatccggag catccgcaac tttgccccaa atgcatcgaa
     2521 gcgttgcaat cacgttaaga gggaatgagg agacgaatcc ttatcgtatg atgttttttc
     2581 atcttagaaa tcccaagact gttttgagga agatcctggt ggggctgatt ctctttttta
     2641 tcggtgccat ggcggcctac cttgtctcat tatcccttcc tcatgtcgtc ggaaatccct
     2701 ggggggctca cccgcttacg ccgattctga cgatgaccct gattttcctc gttgcctaca
     2761 agccggttga gagatgtgtg aaagtgtttc tggaaaaaaa tattttccgg aaaaaaagtt
     2821 atgctcaatg gactctgatg gaacttgcgg aggagcttag gacaaatttg gattttcagg
     2881 agctttccaa ccttattgtc aatacgttcg gagaggccct ccatttgaaa accgtcgcgc
     2941 ttttaatccg ggatgtcact cgggacggtt ttgagattgc ttccgcgttt ggctggcccc
     3001 tggcgacttc acgaagaatt tttctcggaa atgactcgcc cctggtgcgg tgtatccacc
     3061 ggatgggccc gcatgtgctt gtgcgcggcc cgcttcttaa gagcctttcc tggcaggaag
     3121 ccaatgaaat ggcccgtgat tttgatctgg tgcgttcgtg ctgggtgctt cctctttttt
     3181 ccaaaggaga cctgatcgcg atgatcgcgt ttggggcgca ttctcccgat acggtttttg
     3241 atgaatggga cttccatttt ttcaggaagt ttggtgagtc ggtcgcgccc tgtgtggcga
     3301 actcccggta cgttcagggt cttcgacgtg ccaattttga actccaggac tcacaagctg
     3361 aatggttcca gaagacgaaa atgtccgcta ttgagaaact cgcggccggg attgctcatg
     3421 aggttcacaa tcctctcgcg attatttcag ggaaggcgca ggttttgctg atgcaaaaaa
     3481 aacggacccc gctggaaccg catgtcgaag aagctttaaa cgtgatagtg aagcaaacgc
     3541 gtcgggctgc cgatattacc cgaaagcttc tcatgtactc acaggattcc agggcgcccc
     3601 aggaagaaat ttcatttgag aaaatcctgg acgaaaccat tgctttggtg gggtaccagg
     3661 cctcgctcga caagattgaa attattaaga aaacggatcc ttcgctaccg acgttttggg
     3721 gaaacacgca ggaaattcga gaaatctttc ttaacctttt tttgaacgca gttgaggcca
     3781 tcggggacca gggaggacgg atccaggtcg aggttcgtat ttcagaagga gaggatgtta
     3841 ttgaaataag gtttcagaat tcagggaaag agattcccca ggaagatctt gaccggatat
     3901 ttaatccatt ttttacgaca cggcacgaag ccgtgggcct cggtttgttt gtgacgcgtc
     3961 agattgtcaa gcgttacggg ggagcgattc gcgtggaaag tcaaagcggt gaagggagcc
     4021 tttttatcat acaactgccc aggacgactg ccgagggtga aacgccacgc gccagcgcag
     4081 aggaaccgaa agaaggaacg attttagtga acaaaggggc ataacagccg atcgattaaa
     4141 ccaggaggat ccatcatgaa gaaaaaactg ttgctggttg acgatgaagt cgagatatgt
     4201 gagttcctga aatctttttt tgaggatcgt gactaccagg tagcgacggc tcattcaggc
     4261 gttcaagctg ttgaacaagt ggagagtttt aggcctgatg tggttctcct tgatatccag
     4321 atgccggaga tggacggttt gagtgccttg cgagagatta aggaaaaata tccgagggtt
     4381 aaggtgatta tggtgacggc ggttgaaact caggacagga ttgaagcggc catgaggttg
     4441 ggagcggata actatatcac caagcccctg agccttgaat atctcgaaaa ggatgttcaa
     4501 gagaaaatcg agagcctggc cggcatttga gacacgaatg gtatgacaaa tcttacgcgg
     4561 accaatcggc agaaaaaaac ggaacaaaaa tttgattatc aggaagcgct tcgtgacgta
     4621 gcgcggagta tggtgcgcct taaacggccg gatcggttac tcaagcttat cacccgtttt
     4681 attgatcgtc agttcgggct tcatcacacc tcgcttctgg ttcacgaacc ttccaaaaac
     4741 cgctacgtgt ttgtcaattc caaaggagcg cgccgcgttc ccgcgggtct cctcaaattc
     4801 gattcagatc atccgctgat tcgctggttc gcgttgccgc gtcgaaaaac acctttccag
     4861 gaagactatc ttttgttgac ggatataaaa aagacgctta acaaggccca ctttcggctc
     4921 gcggctgatt ctagggcctc ggaattggtg aaagtccaga agactatgga agttctcagg
     4981 gtggaactgg tcattccggg atattttaaa aatgatctgg tgggcatttt gatgctcgga
     5041 ggcaaattta gcgggaggtt ttttacgtct tcggaaattc ttttttttca aacgctcgct
     5101 catgactgtt ctatggccat caagaccgcg gaatataatc aaagtcttct ggagaaaaat
     5161 cgggaacttg aaaacaggct tcaggaaatc gagaagcttc gccacaaaga gcaacggact
     5221 tattacgaaa ttattcgctc tctggcggaa gaggtggaag ccaaggagcc gacggttttt
     5281 ggccatgttt cgcaagtgga aaagcttggc atgatgaccg cccgtgaact tgggattgat
     5341 ctttcgggac aacataagga tatcctttcc gcggctttga tgctccatga tgtggggaaa
     5401 atcggagtgc ccgatcacat tctaatgaaa ccagggagcc ttacccctga ggagtgggaa
     5461 gtgatgaaaa gccatgttga aaagggcgct aaaattcttg aaccgctttc cgattttcgg
     5521 aaagtcaagg agatcattct ttctcaccac gaaaggtttg acgggacggg ctatccccgg
     5581 ggactgaaag gggaagacat ccttatcgag gcccggattg taagcgtcgt tgatagtttc
     5641 cacgcgattg tgtcgaaccg ttgctaccgt gaggggaggt cgatcgattt tgcccttcag
     5701 gaactggagc attgcgcggg tacccagttt gatccccagg tggtggaggc cttcattcgg
     5761 gtcattcgac gagaaatcgc caaaggccgt atcgatgtcg cggcgcttca acattaaaaa
     5821 attttaaaag cttgcagcct ctagattgct tcagtataat gtcgcccgct ttttgagagc
     5881 aggaaggaag cgacatgcct aaaaagaaga aagtaaagaa gaaagctaag aaaaccaaag
     5941 cccgaagggt tgtgaagccg aaagccaagg ttgccaaggg taagaaaggc agtaaaaggc
     6001 acaaggctcc tagggaaagt aaggtagtcc cgatcatggg ttacagggtg gatcggattg
     6061 tcaaggtccc caagggcccc cagcttaaaa aaaccccgtt ttccaagtcg gaacttggtg
     6121 tctttaccaa acttctgatg gatctcaaga ggaggattgt ggggagcctt cagtcaattg
     6181 agaaggaaaa tctgaatact ggccggaagg attccacggg ggggttctca ggttatagct
     6241 ttcacgtgac tgatgcggcg agtgacagtt ttgaccggga gctcaatatc gggcttgccg
     6301 cgaacagcca gcaactttta aatgacatcg cagtcgcgtt gagaaagatc gaagaaggca
     6361 cttttggaat ttgtgaaaaa tatggtatcg ccataccgaa gaaacggctt ctagcggcgc
     6421 cttatgttcg tttgtgcctc caggcccagg aagaagaaga gagggagcct cgccgcagtt
     6481 gatcttcttc tacggactgg tcgggtttgt cgtctttctg gatcaactca ccaaatattt
     6541 cgcgctggtc tttctttccg aggtttccca acctgttctc ccgaatgttt ttcacctgac
     6601 gcttgttcga aatacgggcg tggcctttgg ttttttgagc gaccacagtt ctcttctttt
     6661 ttccgtgatt acggtgagcc ttgtcttcct gtttattctg gtggcaaggt ctcacggagc
     6721 cagtctttct gaaaaatggg ctcttagcct catcctcggg ggcgcgatcg gtaac
//
LOCUS       DAOSIO010000078         6757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_10245_length_6757_cov_3.835273, whole genome shotgun sequence.
ACCESSION   DAOSIO010000078 DAOSIO010000000
VERSION     DAOSIO010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10245_length_6757_cov_3.835273"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1329)
                     /gene="wecB"
                     /locus_tag="PK590_04045"
     CDS             complement(136..1329)
                     /gene="wecB"
                     /locus_tag="PK590_04045"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOG23800.1"
                     /translation="MKTERRGKGERRIGRNEIHILHVVGARPNFMKVAPVMRALERYS
                     ATRQSLVHTGQHYDDNMSRIFFSDLGLPAPTVNLKVGSGSHARQTSEIMVRLESEIVK
                     RKPDLVLAYGDVNSTVAAALVCSKLGVLFGHVEAGLRSFDRSMPEEINRLLTDQISDF
                     LFTPSLDGNKNLLREGVSKEKIYFVGNVMIDTLVRLLPKARARWREPGFFPDITGENL
                     ERYGLVTLHRPSNVDEMGLLSSIMRSLNKISRSLPLVFPVHPRTRKQFESLRFKQSQQ
                     LYFSAPLSYVDFLALQCRATLVITDSGGIQEETTYLGVPCLTLRKNTERPVTVTKGTN
                     ILVGQDMENLLAAVEKILKNPHKKRNIPALWDGKASERIAKTIIRSRLFARRDMCCSS
                     QTAQW"
     gene            complement(1629..2783)
                     /locus_tag="PK590_04050"
     CDS             complement(1629..2783)
                     /locus_tag="PK590_04050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG23801.1"
                     /translation="MTDMQEKKPRAVMLSPALSQPRHHRRAAMLLEAGYRLQVYGYQR
                     GYYEENMYPDGIQTISLGRIESKYYIRRIPKLLKSALFIKKYEKQFHEAPSLIYAFGL
                     DMALIESFAMSGSVPLIYEIGDIQNPLPHTGIVSKMIAITEKLILRKCRKLVVTSPAF
                     IPGYFERLDLSIAKKTLVIENKLARENARRFPRPQAPKQATFPIKIGCIGFFTYRNCI
                     LALMEAVVKKRGKFELHFYGDGPLKKEIREYAKTHDNVFYHGSFSNSADLGKIYQSVD
                     LSYVVYDNTDPNVRMALPNKLYEGPYFGVPLVVAKDTFLAERVRELGIGFVIDPQEEN
                     SLDKLMDEITPEILRKLSATALGLDKSHFVEDGSGVIPVFEEIGKSRSLQ"
     gene            3385..4503
                     /locus_tag="PK590_04055"
     CDS             3385..4503
                     /locus_tag="PK590_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOG23802.1"
                     /translation="MGKIKVAIAGCGNCAASLLEGLEYYRRITAEKLKGKKKEVGCLG
                     LMNDDMGGYFPNDIEVVAAFDIDKRKVGERLDAALRAKPNCVLKLCDIARSKVRVEMG
                     HVLDGVFEHMQNYADDRTFIVANKKPANVEKILKESGAEILLNYLPVGSQKATEFYAE
                     ACLKTGVSLINCMPVFIVSNPAWAKRFEAKRIPCVGDDIKAQIGATITHRTLAKLFSE
                     RGVTIDRTYQLNTGGNTDFLNMLNRARLESKKISKTSAVQSQLDVPLDPDNIHIGPSD
                     YVPWQNDNKVCYLRIEGRGFAGFPVSLECRLSVEDSPNSGGCAIDAIRACKIARDRKI
                     GGPLISISAYTMKHPPKQIVDYEARELVRKFVQGTCPR"
     gene            4516..5115
                     /locus_tag="PK590_04060"
     CDS             4516..5115
                     /locus_tag="PK590_04060"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOG23803.1"
                     /translation="MLRKKVYPKIENLVTLWVDWLSKNGITPHQLTFAGAAVSLLAGI
                     IYAKGYLLLGGIVLLIAGLGDMLDGPLARSTGKASSFGAFLDSSVDRYSDFFIFGGIA
                     VYFSRRGDVFWFLVALGVILGAFVVSYTKARAENFIKDCGVGLFGRAERIVLLAIGTL
                     ISPLLKLVLLVILVGTHVTAIQRILHTKKNLAETKGPLH"
     gene            5172..5996
                     /locus_tag="PK590_04065"
     CDS             5172..5996
                     /locus_tag="PK590_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23804.1"
                     /translation="MPVQGYALSPSSAPALETLLKQIENRHYRWVAFRADVVLFFVAP
                     GANGQAMCGGELIYERLQERMLLSCQDSEENTVFVFRTLDRLFDLYIPSQNTLYHGSI
                     FDLEDSPDVESHLKMMDLYRALKPGMFDPNRSQVESSSGVSISLNVFSGPENGAPLSR
                     KIYLTPQGDVLGELFFDQSGQVVTEIQRYDFREIPSRSGGFETVIFPKKVTVVSPRTG
                     RNTAIFFSNIKPLDTVNSYQFLLRIPKGTREVYLREVDPRFAETAPASSAQSEADH"
     gene            complement(6074..6514)
                     /locus_tag="PK590_04070"
     CDS             complement(6074..6514)
                     /locus_tag="PK590_04070"
                     /inference="COORDINATES: protein motif:HMM:NF021486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 family protein"
                     /protein_id="HOG23805.1"
                     /translation="MIRKKQKLFRLSEANQLLPEIGRRLKQIQQKKESYSLIHDELFM
                     HDLVSSAERSNGFAEKDDLETRVRDLEQAIEGLAKDVEAIFATGCILRDIEKGRVDFP
                     AEVENKRIFWSWELGEPMVRYYRHLKSKFSERILIPARLLKTAK"
BASE COUNT         1673 a         1779 c         1526 g         1779 t
ORIGIN      
        1 tcaattccac gcgagacgca cacttcgcca gtttctcaga cattatcatg cgtatcgttg
       61 tttcaaggtt ttttcgcata atctgccgtg tcaccttaac gctgatcctg atgagttgga
      121 ggaaccactt atgtgctacc attgggccgt ttgactggag caacacatat cccgcctggc
      181 aaagagtctg gatcgaatga tggtctttgc tattctttcg gaagcttttc catcccagag
      241 agcaggaata tttcttttct tgtgaggatt ttttaatatt ttttctaccg cggcgagcaa
      301 attctccata tcctgtccca caagaatatt cgtccccttc gtcacggtaa caggcctctc
      361 cgtattttta cgcaaagtta aacaggggac gcccaaatag gtcgtttctt cctggatacc
      421 gcccgaatca gtgatgacaa gcgttgcgcg acactgtaac gccaagaaat ctacgtaact
      481 caagggagcg gaaaaataca actgctgact ttgtttaaaa cggagagatt caaattgttt
      541 ccgggtccgg ggatgaaccg ggaaaacaag cggcaaagac cggctgatct tgttcaggct
      601 acgcatgatt gaactcaaca aacccatctc gtccacattg gaaggacggt gaagggtgac
      661 aagaccatat cgttccaagt tttctcctgt tatgtccgga aaaaaacctg gttccctcca
      721 ccgcgcccgg gctttcggca agaggcgaac cagggtatca atcatcacat tccccacaaa
      781 ataaattttt tcttttgaaa ctccttctcg caaaagattt ttgtttccat caagagatgg
      841 cgtaaaaagg aaatccgaga tctgatccgt cagcaaccga ttgatctctt ccggcataga
      901 acgatcaaaa gacctcaatc ccgcctcaac gtgcccaaac aggacaccca gttttgagca
      961 tacaagcgcc gccgcaacgg ttgaattgac atcaccatag gccaatacca gatctggctt
     1021 gcgtttcaca atctcagatt ccagccggac catgatttca gaggtttgac gagcgtggct
     1081 ccccgagccc actttcaaat tgacggttgg tgctggcaac ccaaggtcgc tgaaaaatat
     1141 ccgggacata ttgtcatcat aatgctggcc tgtatgaacc aggctttgcc tcgtcgctga
     1201 atatcgctcc agcgcgcgca tgacgggagc aactttcata aaatttggcc gcgctccaac
     1261 cacatgcaaa atatgaattt cgttacgtcc gatcctgcgt tctcccttgc ctcttctttc
     1321 agttttcata tgttccccac tcaccgttca acaaaaaaac agcctcactt tcatgttcaa
     1381 cacttacagc tatcaccaac cagttctctc ccggttagaa aaacccctca cttatagtcg
     1441 gaaaaaatga cgcaaatgcc caggggaagt aacactacta tctcgtcata agcggatcaa
     1501 ctctaatcga ttgtgttctt tggtgttcta aaccacaaac gccgtgtcac ttagcccatt
     1561 gattcagcgc tccttttagc tcaaggggaa aattccatag cgtctcatgg ctaaaaccca
     1621 ctccttaact actgcaacga tctacttttc cctatttcct caaagacggg tatcacacca
     1681 ctaccgtctt ctacaaaatg agacttgtct agaccaagag ctgttgcgct taactttctc
     1741 aggatttcgg gagtgatttc atccattaac ttgtctagag aattctcctc ctgaggatct
     1801 atgacaaaac cgatcccaag ctctctgact cgttcggcta aaaatgtatc ttttgccacc
     1861 acgagcggca ctccgaaata agggccttca tataatttgt tcggaagagc cattcttaca
     1921 tttggatctg tgttatcgta aactacatag ctcaaatcga ccgattgata tatctttcca
     1981 agatccgcag aatttgaaaa ggatccatgg tagaaaacat tgtcatgagt tttggcatat
     2041 tcgcgaattt ctttctttaa aggaccatcg ccataaaagt gcagctcgaa cttccccctt
     2101 ttttttacca ccgcttccat caaagccaaa atacaattcc tgtatgtgaa gaaacctatg
     2161 catccgatct tgattggaaa cgtagcctgt ttgggcgctt gaggccttgg aaatcttcgg
     2221 gcattttcgc gagcaagttt attttcaatg accagtgttt ttttagctat gcttaaatcc
     2281 agccgctcaa agtaaccggg tataaaggca ggtgacgtga ctaccagttt tctgcatttg
     2341 cgcagtatta atttctcagt aattgctatc atttttgaca caatccctgt gtgcggtaaa
     2401 gggttttgaa tgtctcctat ttcataaatc agtggaacag atccggacat tgcaaaactt
     2461 tctatcaaag ccatatccag cccaaacgca tagatcagtg aaggagcctc atggaactgt
     2521 ttctcatatt ttttaatgaa taatgcggat tttaagagct tggggattcg tcttatataa
     2581 tattttgatt caattcgtcc aaggctgata gtctggattc catcaggata catattttct
     2641 tcataatatc cgcgttggta tccataaact tgcaggcgat acccagcctc caacaacata
     2701 gccgcgcgcc gatgatggcg aggttgcgat aaggctgggg acaacataac cgctcttggt
     2761 tttttctctt gcatatcagt catcgatgat ccgtaacact gttactttta ctccttatga
     2821 agccgctaca atgtggatag atactatgaa attcataaag aacttccttc gatctcgccg
     2881 tatcgttcgg gacaagatag tatatcgtca aatcttttcg gtgcaattcc ctaactgagc
     2941 gaggtggctt cggcaattta acgcaacgct tgcgaaatat aataaccgca aggtgatgtt
     3001 atgaaaagga gagaaaagag aatctcgcgg cctcacgatg cctcgcatgg taaggtttta
     3061 atatgaaaca accggacatc gcttggtatg aggccaaaaa gcaaaagatc gcgggattcg
     3121 tcccgccggg cccttgctta gcctccggag agccggtctg gataccttgt ttacaaggca
     3181 actattacca tttccggatt taaaatctgg tcacgttata aaatataatt ttttataaag
     3241 tcggacgtat caaccgtaac atattataaa taatgatctt atggctcgtc gctttcgctt
     3301 gatttcggat tgattggtgc tatcataata ttttacttta attctcgccg ttttcattca
     3361 ccaatgaagg aggtttcaga atccatggga aagatcaagg tcgctatcgc gggctgtggc
     3421 aattgcgccg cgtcattact tgaagggctt gaatactatc gccgcataac cgcagaaaag
     3481 ctgaagggaa agaaaaaaga ggttggctgt ctggggttga tgaacgacga catgggcggg
     3541 tactttccca atgatatcga ggtggtggcg gcttttgaca tcgacaagcg caaggtgggc
     3601 gaacgcctcg atgccgccct ccgggcgaag cccaattgcg tcctcaagct ttgcgatatc
     3661 gcgcgaagca aagtccgggt cgaaatggga cacgtcctcg acggcgtttt cgagcatatg
     3721 caaaactacg ccgatgaccg cacttttatc gtcgccaaca aaaaaccggc taacgtcgaa
     3781 aagatcctta aagaaagcgg cgcggaaatc ctgctcaact atctgcccgt cggttcgcaa
     3841 aaagccaccg aattttacgc cgaggcctgt ttgaaaaccg gcgtgagcct tatcaactgc
     3901 atgccggttt ttatcgtttc caatccggca tgggccaagc ggtttgaggc gaaacgaatc
     3961 ccctgcgtcg gcgacgacat caaggcccag atcggagcga cgatcacgca tcgtaccctc
     4021 gccaaactct tcagcgagcg cggtgtcacc atcgaccgca cgtaccagct caacaccgga
     4081 ggcaatacgg acttcctgaa catgctcaat cgggcccggc tcgaatccaa aaaaatctca
     4141 aaaacttccg cagtccagag ccagcttgac gttcccctgg atcccgacaa tatccacatc
     4201 ggcccttccg attacgttcc atggcaaaac gataataaag tctgctattt gcgaattgag
     4261 ggccgcggtt tcgcgggatt tcccgtctcg ctggaatgcc ggctctccgt ggaagattct
     4321 cccaatagcg gcggttgcgc gattgacgcc atccgcgctt gcaaaattgc cagagatcgc
     4381 aaaattggcg ggcccctgat ctccatttcg gcttacacga tgaaacaccc gccgaaacaa
     4441 attgtggatt acgaggcaag agaactggtt cgaaaatttg ttcagggcac ctgccccaga
     4501 taaggctggc ccaacatgct gagaaaaaaa gtttatccca aaattgaaaa tctggttacc
     4561 ttgtgggtcg actggctgtc caaaaacggc attacccccc accagctcac gtttgccggc
     4621 gcggcggtaa gtttactcgc gggaattatt tacgcgaaag gctatcttct gctgggcggt
     4681 atcgtcctgc ttatcgcggg tttgggagac atgctggacg gccctctcgc ccgctccacc
     4741 ggcaaggcaa gctcttttgg agcttttctc gattcgagcg tggatcgtta ttccgatttt
     4801 tttattttcg ggggaattgc ggtttatttt tcccggcggg gagacgtctt ctggtttctc
     4861 gtcgccttgg gggtcatcct cggagctttt gtcgtcagtt acaccaaggc aagggccgag
     4921 aacttcatca aggattgcgg tgtcggattg ttcgggcgcg ccgagaggat cgtattgctg
     4981 gccatcggga cactcatttc ccccttgctg aaactcgttc ttctcgtgat tttggtcggc
     5041 actcacgtca ccgccataca aaggatcctt cacaccaaaa aaaatctggc ggagaccaaa
     5101 ggcccgcttc attaatgccg ctccggatcc ggaatttcct ttcttgtttt ctcacgatcc
     5161 tttcctgctt tatgcccgtg cagggttacg ccttgtcccc atcctccgct cccgccctgg
     5221 aaacactgct taaacaaatc gaaaatcgtc attaccgatg ggtcgcgttt cgggctgatg
     5281 ttgtcctttt tttcgtcgct ccgggcgcta acggacaagc catgtgcggc ggagagctca
     5341 tttatgaacg ccttcaggag cgaatgcttc tttcctgcca ggactccgaa gaaaataccg
     5401 tctttgtctt cagaaccctg gaccggcttt ttgaccttta tatcccgtca caaaatacgc
     5461 tctaccacgg ctccattttt gacctcgagg attctccgga cgtagagagc cacctcaaaa
     5521 tgatggacct ttatcgggcc ctcaagcccg gcatgttcga cccgaaccgt tcccaggtcg
     5581 aaagttcttc gggtgtgtcc atcagcctga acgttttttc agggccggaa aacggggccc
     5641 ctctttcccg gaaaatttat ctgacgcccc aaggcgacgt gctcggagag cttttttttg
     5701 accagagcgg acaggtcgta accgaaattc agaggtatga tttcagggaa atcccttcgc
     5761 gttcgggggg atttgagacc gtcattttcc ccaaaaaagt caccgttgtc tctccccgga
     5821 cgggccggaa cacggctatt tttttcagta atatcaagcc cctcgatacc gtcaattcct
     5881 accagttcct gctgagaatt ccgaaaggaa cccgggaggt ttacctgcgg gaagtggacc
     5941 ctaggttcgc ggagacggcg cccgcgtctt cggcgcaaag cgaggctgat cactgaaaaa
     6001 gtttttcgcc gtccgcgcaa aatcagccgc ttgccgcgca agcggccctc gcgagatctt
     6061 ttttccggca ggtttatttg gcggttttga ggagccgcgc ggggatcaaa attcgttccg
     6121 aaaatttgct tttcaggtga cggtaatagc gaaccatcgg ctctcccagt tcccaggacc
     6181 aaaaaatccg cttgttctcc acttcagccg gaaaatcaac ccgtcctttt tctatgtcac
     6241 gaagaatgca tcccgtcgca aatatcgcct ccacatcctt ggccaggccc tcaatcgcct
     6301 gctcaaggtc ccggacacgg gtttcgaggt cgtctttttc cgcgaagccg tttgagcgtt
     6361 cagctgacga taccaggtcg tgcataaaaa gctcatcatg aatgagcgag taggattctt
     6421 ttttctgctg aatttgtttg agccgtcgcc cgatttcggg aaggagttga ttcgcttcgg
     6481 acagccggaa cagtttttgt ttctttcgta tcacgttttt acgtctccgt agggttaaca
     6541 tacacccgct ttttttgaaa atcaaatctc aaaaaattct ccggcgccga aaccttccgg
     6601 gaccgaaaac tatgaggatg gacgcattgg atccgccgct tcctcccccg ggaaaacgtc
     6661 caaattcacg cgccttcccc gccctcttcc tcaggcggta aaactatccg gggccacatc
     6721 cgtccacacc gccggcataa gcccgccccc ggtcgcg
//
LOCUS       DAOSIO010000079         6737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_10282_length_6737_cov_3.748279, whole genome shotgun sequence.
ACCESSION   DAOSIO010000079 DAOSIO010000000
VERSION     DAOSIO010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6737
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10282_length_6737_cov_3.748279"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PK590_04075"
     CDS             complement(<1..643)
                     /locus_tag="PK590_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011398586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HOG23806.1"
                     /translation="MRTCKRCLLPETYETIDIAEDESGCNLCGSVDKKRQSINWTKKK
                     RMLDSIIEKYRGKYEYDCIVPFSGGKDSCYALYYLVKEYKIKPLVVRFDSGFMRPITA
                     ENIRTVIKKTGVDYISFTPNWHVVKLLMLEAFQKKTDFCWHCHTGVYSYPLRIAVKYN
                     TPLVFWGETMDLMLGGYDFTDQSIDFEDEKRFNSARTLGISAMDMYQSLKKKGV"
     gene            complement(640..1362)
                     /locus_tag="PK590_04080"
     CDS             complement(640..1362)
                     /locus_tag="PK590_04080"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016478173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HOG23807.1"
                     /translation="MYKNKRILAVIPARGGSKGLPRKNIKDLNGKPLIAWTIEQASHC
                     KLIDEIFVSTDCPEIAKISEQWGIIVPSLRPAHLASDTASSIDVLLFTIDLLEKAGKR
                     FDLIALLEPTSPLRDSEDIDAAITMLVETKNAGSVMSVCRAEASHPDFLTRITEDQFL
                     RPFGREKFVFKRRQEIERLYFFEGSLYISYVDALKKEKSFCHQKTLGYEVAKYKSFEV
                     DDAIDFKIVEALLKTKQSGELI"
     gene            complement(1401..2453)
                     /locus_tag="PK590_04085"
     CDS             complement(1401..2453)
                     /locus_tag="PK590_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOG23808.1"
                     /translation="MTDWNLSLLKDSATIKESLIRINLFSSNQSLILFVLNDKRQVIG
                     SLTDGDIRRGLLSGLGLSDSVIKVMNKQYHFLRKDSSVAEIRQMRSKELHVVPILDSS
                     RRIIRFIDFKKTHSVLPLDAIIMAGGKGIRLRPYTNDIPKPMLELNGKPIIAHNIDRL
                     ITYGVRNFYISVNHLKHKIIRYLNRAYQGREISIRYIKEKKPLGTMGSVALVPKYEHS
                     DLLVMNGDILTNINFEDFYVTYKTLNNDIAVATFNIKVDVPYAVLNTFKKRICSFVEK
                     PTYVYYSNAGIYLFRKEHIKLIPKNKPYDAIDLVKGLIKHRKKATHFPIRGYWLDIGT
                     LKSYRKAQDDINHIQF"
     gene            complement(2459..3064)
                     /locus_tag="PK590_04090"
     CDS             complement(2459..3064)
                     /locus_tag="PK590_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HOG23809.1"
                     /translation="MMKKPVIVLGGGGHAKVLIDALRLSGVKVLGYTDRSKEAGHALG
                     VPFLGGDERVLDFSPKKVLLVNGVGSVESTKKRRDLFRSFKDKGYQFASVIHRSAVLA
                     KTVKLGEGVQVMAGAVIQTGTVIGDNAIINTKASVDHDCRIGVHVHIAPGVVLSGGVK
                     IGFGSHIGVGAIIVQNVVVGNRSFVKAASLVTRTYRSANTR"
     gene            complement(3058..4143)
                     /gene="neuB"
                     /locus_tag="PK590_04095"
     CDS             complement(3058..4143)
                     /gene="neuB"
                     /locus_tag="PK590_04095"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007727142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HOG23810.1"
                     /translation="MRRERAYIIAEAGVNHNGSLRLAKRLVDVAKYAGADAVKFQSFH
                     AEEIATANVPKANYQSKNLSGGCGQLEMLKKLELSVAKQRALFFYCRKKKIEFLSTPF
                     DLKSLAFLIKLGVKRLKISSGELTNGPLLLAAARSGLPLILSTGMSDLAEIRDALDVI
                     AFGYLRTGNARPPHSLKMRRLARTTCAKLKLAKNVTLLQCTSEYPTPMEDINLRAMDA
                     LREKFGVSAGLSDHSTGIAVPIAAAARGARIIEKHFTLDRNSPGPDHQASLEPDELKL
                     MVKAIREIERALGDGHKRPARSESKNMAVVRRSIVAARTIAAGEEFSELNLTVKRAGK
                     GISPMLIWSFYGKRASRCFSKDERVTL"
     gene            complement(4145..5296)
                     /gene="neuC"
                     /locus_tag="PK590_04100"
     CDS             complement(4145..5296)
                     /gene="neuC"
                     /locus_tag="PK590_04100"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HOG23811.1"
                     /translation="MKKRKICVVTGSRAEYGLLHCLMKEIRKDRSFHLQVIVTGTHLS
                     AVFGSTYRDIERNGFKIARKIPILTSDNSATGVTQALGKAVIGYAKAFKDLKPDWLVV
                     LGDRYEILAAVEAALIAKVPVAHLAGGDITEGAIDDSIRHAITKMSHLHFATHAAAAR
                     RIRQLGEDPKTVFCPGHIGLDAIRLLKPESRRDLERDLRFRFRERNLLVTFHPVTLDP
                     VPSMEQLEELLAALHGLKRDMGLIFTASNADADGSKMNLRIRRFVERHPNACFIHSLG
                     QRRYLSTLRYVDAVVGNSSSGIYEAPSFRIPTINIGSRQEGRVQAKSVINCLAKRVFI
                     QRAIEKAFLMDCSRVVNPYGDGRATARIIRSIKSIRDPKSLLKKKFRSL"
     gene            complement(5293..6468)
                     /locus_tag="PK590_04105"
     CDS             complement(5293..6468)
                     /locus_tag="PK590_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009753722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HOG23812.1"
                     /translation="MQHVNSKLNKDAVLKSIRSCLPEGRRDIELHEPYFCGNEWKYVK
                     ECLDTNWVSSVGKFVDTFEQKLAEYTGAKRAFAVVNGTAALHISLKMAGVESGDEVLV
                     PDLTFVATANAVTYCGAIPHFVDCESVSLGVDARALEKYLAGIAEIRGSVCCNRSTGR
                     AIKVLVAMHTFGHPSDLDAIQTVCEKYQITLIEDAAEALGSFYKDRHVGHHGKFAILS
                     FNGNKTLTTGGGGAILTNDEKLGLKAKHIATTAKLPHPFLFFHDQIGYNYRMPNINAA
                     LGVAQLENLPAFLRNKRALAMRYKEAFNDFTGVHFVDEPAHGKSNFWLNAIMLDAELA
                     EERDALLGHLRSKGIKARPAWMLMHKLPMFQDCPRMDVPVAEDIESRLINIPSSAFL"
     gene            complement(6456..>6737)
                     /locus_tag="PK590_04110"
     CDS             complement(6456..>6737)
                     /locus_tag="PK590_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HOG23813.1"
                     /translation="FGSNFEISIGDVARTIAEIMNSKIKMTKDNARIRPAKSEVERLW
                     ADHTKARKLTGWMPAYAGFAGFKRGLTETIKWFKESGHLAQYKSHLCNM"
BASE COUNT         1779 a         1740 c         1464 g         1754 t
ORIGIN      
        1 cgaccccttt tttcttcaag gattggtaca tatccatggc ggaaattcca agcgtccggg
       61 ccgagttgaa ccttttttca tcctcaaaat cgatactttg gtcagtaaaa tcataccctc
      121 ccaacatcaa gtccatcgtt tcgccccaaa acaccaaagg cgtattatat ttaacggcga
      181 ttcgcagcgg gtaagaatag acgcccgtat ggcaatgcca gcaaaagtca gtcttctttt
      241 gaaaagcttc cagcataagc aatttaacga catgccaatt cggagtaaaa ctaatatagt
      301 caacaccggt ttttttgata acggtacgta tattctcagc tgttatgggc cgcataaaac
      361 cggagtcaaa gcgaacgact aagggtttta ttttgtattc tttcacgaga taataaaggg
      421 cataacaact gtccttaccg cccgaaaaag gaacaatgca atcatactca tatttacctc
      481 tatatttttc tatgatagag tcgagcattc gctttttttt agtccagttg atgctttggc
      541 gctttttatc cacacttccg cacaaattgc agcccgactc atcctctgca atatctatag
      601 tttcataagt ttctggaagc aaacacctct tgcaagttct catataagct ctccgctctg
      661 cttcgttttt aacaaagcct ctacgatttt aaaatcgatg gcatcatcca cttcaaaaga
      721 tttatacttc gccacttcat atcccagcgt tttttgatgg caaaaacttt tctctttttt
      781 taacgcgtca acataagaaa tatataaact accttcgaaa aaatataagc gttcaatttc
      841 ctgtcttctt ttaaacacaa atttttcacg gccgaaaggc ctgaggaatt ggtcctcagt
      901 tattcgagtc aagaaatcag gatgagacgc ttcagcgcga catacactca taacgcttcc
      961 ggcatttttt gtttcaacga gcatggtgat agccgcatca atatcttcac tgtcgcgcag
     1021 aggcgatgtg ggttctagca aggctatcaa atcaaaacgc ttgcccgcct tctctagcaa
     1081 atcaatagta aacaataaaa catcaataga agaagccgtg tccgatgcaa gatgagcagg
     1141 cctcaatgag ggcacaatga tgccccattg ctctgaaatc ttggcaatct ccggacaatc
     1201 agtggagaca aatatttcat caataagttt gcaatggctg gcttgctcga tcgtccaagc
     1261 tattaatggc ttcccgttca aatctttgat gttttttctt ggcaatccct tagacccgcc
     1321 acgagccgga attaccgcta aaatacgttt gtttttatac ataccttaac tcttatttta
     1381 aagcactagc ttttttgtgg tcagaactgt atgtggttaa tatcgtcctg cgccttccga
     1441 taagacttga gagtaccgat atccagccaa tagcctcgga tcggaaaatg ggtcgctttt
     1501 tttcgatgtt taattaatcc ttttactaaa tcaatagcgt catagggttt attttttggg
     1561 atcagcttaa tgtgctcttt cctgaaaaga taaattccgg cattagaata ataaacatac
     1621 gtaggcttct cgacaaaaga gcaaattctt tttttgaaag tattcaggac agcgtaagga
     1681 acatcgacct taatattaaa agtggcaacc gcaatatcgt tattcaaagt tttatacgtc
     1741 acataaaaat cttcaaagtt gatattggtt aatatgtcgc cgttcataac taaaagatcc
     1801 gagtgctcat atttggggac gagcgcgaca gaacccattg ttccaagcgg ctttttttcc
     1861 ttaatataac gaatcgaaat ttctcttcct tgataagcgc gatttaaata gcgaattatt
     1921 ttatgcttta aatgattgac tgaaatgtag aagtttctaa caccgtaagt tattagccga
     1981 tctatattat gagctataat cggttttcca ttcagttcca acataggttt tggaatatca
     2041 tttgtatagg gcctcaagcg aatgcctttc cctccagcca tgatgattgc atcaagcggt
     2101 aaaacagaat gcgttttttt aaagtctatg aatctgatta ttcttcgact gctgtctagg
     2161 atcggaacaa catgaagctc tttcgatcgc atttgccgaa tttcggcgac ggaggaatcc
     2221 tttcttaaaa aatggtattg cttgttcatg acctttatca ccgaatcgga taaaccaagt
     2281 ccagacagaa gcccgcgccg aatatcccca tcagtaagag agccaatcac ttgccgctta
     2341 tcattcagca caaaaagtat aagcgactga ttcgaggaaa aaagattaat ccggatcaac
     2401 gactctttaa tagtggctga gtcttttagt aatgatagat tccaatcagt catttgtatt
     2461 acctcgtatt tgctgaacga tacgtccgtg tcactaacga agcagcttta acaaaggatc
     2521 tattgcccac gacgacattc tgaacaatga tcgcgcctac tcctatatgg cttccaaacc
     2581 caatctttac gcctcccgat agcacaacac cgggggcgat atggacgtgc accccaatgc
     2641 ggcaatcatg atccacggag gctttggtat tgatgatcgc attgtcaccg atgaccgtcc
     2701 ctgtctgaat gaccgctccg gccataacct gaacgccttc gcccaacttg accgtctttg
     2761 ccaggaccgc agaccgatga atgacgctcg caaactggta gcctttatct ttgaaagacc
     2821 ggaatagatc acggcgcttt ttcgtggatt ccaccgaacc gacaccattc accaagagaa
     2881 ccttcttcgg agaaaaatca agaacgcgct catctccgcc gagaaagggc acgcccagag
     2941 catgaccggc ttccttgcta cggtccgtat atccgagaac cttgacaccg ctcagccgca
     3001 gcgcatcgat caggaccttc gcgtggccac cgccgccaag cacgatcacg ggctttttca
     3061 taatgtcacc cgctcatctt ttgagaaaca acgggacgct cgttttccgt agaaagacca
     3121 gatcagcatg ggtgaaatcc cttttccggc ccgttttacg gtcaagttca attcggagaa
     3181 ttcctctcca gcagcgattg tccgcgccgc gacaatagac cggcgaacaa ccgccatgtt
     3241 ctttgattca gaacgcgccg gccgcttatg gccatcgcct agcgcccgtt cgatctcccg
     3301 gattgctttc accataagct ttagttcatc cggttccagc gaggcctggt gatcaggccc
     3361 cggtgaattc cggtcgagtg taaaatgttt ttcaatgatc cgcgcacctc gggccgccgc
     3421 ggcgatcggc accgcgatac cggtactgtg gtccgacaac ccggccgaga caccgaattt
     3481 ttcacgcaga gcgtccatgg cgcgaagatt gatatcctcc atcggcgtcg gatactcgga
     3541 tgtacattga agcaaagtaa cattcttcgc caatttaagt tttgcgcagg ttgtacgcgc
     3601 caatcgccgc atttttaatg aatggggcgg tcgtgcatta cccgttctaa gatacccaaa
     3661 agcgatcacg tcaagcgcat ctcgaatctc tgcgagatcc gacatccccg tcgaaagaat
     3721 gagcggcaat ccactccgcg ctgcggcaag aagcaagggg ccattcgtaa gttcgcctga
     3781 ggagatcttt aaacgcttaa caccaagctt tataagaaac gccaaactct tgagatcaaa
     3841 aggggtcgac aaaaattcga ttttcttttt cctacaataa aaaaaaagcg cgcgctgctt
     3901 cgccacggag agctccaatt tttttaacat ttcgagctgt ccgcaaccgc cagaaagatt
     3961 cttcgattgg taattagctt ttggaacatt tgctgtcgcg atctcttcgg catgaaaact
     4021 ctgaaattta acagcatctg ctcccgcata ctttgcgaca tccacaagcc gtttggctaa
     4081 tcgcaaagag ccattatggt tcacgccggc ctcggcaata atatacgcac gctctcgtct
     4141 catgtcagag gctccggaat tttttcttca ataaagattt cggatcgcga atgctcttta
     4201 tcgagcggat gatccgggcc gtggcccgcc cgtccccgta tggattgacg acacgcgaac
     4261 aatccatcaa aaaggctttc tcgatggccc gttggataaa aacacgtttc gccagacagt
     4321 tgataaccga ctttgcctga acgcgccctt cctggcggga accgatattg atggtaggga
     4381 tccggaacga tggcgcttca tagatcccgc ttgatgaatt cccgacaacg gcatcaacgt
     4441 agcgaagcgt gctcaagtag cgccgctgac ctaacgaatg aatgaaacaa gcgttcgggt
     4501 gacgctcaac aaatcggcgt atcctgaggt tcatcttcga accgtccgca tcggcgtttg
     4561 acgcggtaaa gatgagcccc atatcccgtt tcaagccatg caacgcggcc aggagctctt
     4621 cgagctgttc cattgacggc accgggtcga gagtcacggg atggaatgtc accagcaaat
     4681 tcctctcacg aaaccggaat cggagatccc tttcaagatc gcgacggctt tcgggcttca
     4741 gtagccggat cgcgtcaagg ccgatatgcc ccgggcaaaa aaccgttttg ggatcttcgc
     4801 ccagttgacg tattcttcgg gccgctgccg catgagtcgc aaaatgcaga tgagacatct
     4861 ttgtgatcgc atggcggata gaatcatcga tagcaccctc ggtgatatcc cctcccgcaa
     4921 gatgggccac agggactttg gcaattaaag ccgcttcgac cgccgccagg atctcatagc
     4981 ggtccccaag cacaaccagc cagtcaggtt ttaaatcctt aaaggctttc gcataaccta
     5041 tgacagcttt tccgagagct tgggtgactc ccgtcgcgga gttatcggag gtcaggatcg
     5101 ggatcttccg ggcgatctta aagccgttcc tttcgatgtc gcgataggtt gatccgaaga
     5161 ccgcagaaag atgcgtcccg gtgacaatga cctggagatg gaacgaccgg tctttacgaa
     5221 tctctttcat taagcaatga aggagaccgt attcagctct tgaacctgtc acaacacaaa
     5281 tttttctttt tttcataaga acgcactgct cggaatgttg atgagacgcg attcaatgtc
     5341 ttcggcgacg ggaacatcca tgcgcggaca atcctggaac atcggcaact tgtgcataag
     5401 catccacgca ggacgggcct tgataccctt cgagcgcaag tggccaagca aagcatcccg
     5461 ttcttcggcc aactcagcat ccagcatgat ggcattcaac cagaaattac ttttaccgtg
     5521 tgccggctca tcgacaaaat gaacaccggt aaaatcatta aacgcttcct tgtaacgcat
     5581 cgcaagagca cgcttgttcc ggagaaacgc aggaagattt tcaagctggg cgacgccaag
     5641 ggcggcattg atgtttggca tgcggtagtt gtacccgatc tggtcatgaa aaaacaaaaa
     5701 gggatgaggc aacttcgcgg tcgtggcaat atgttttgct ttgaggccaa gtttctcgtc
     5761 attagtcagg atcgcaccgc ctcccccggt cgtaagcgtc ttattgccgt taaaactaag
     5821 gatggcgaac tttccatgat gcccgacatg gcggtccttg taaaaagaac caagcgcttc
     5881 ggccgcgtct tcaataagcg tgatctgata tttttcacac accgtttgaa tggcatcaag
     5941 gtccgaagga tgcccgaacg tatgcatcgc gaccaaaacc ttaatcgcgc gtccggtgct
     6001 tcggttgcag caaacgctac cgcggatttc agcaatgccg gcaagatact tctccaacgc
     6061 cctcgcatct accccgaggc tgacggattc gcagtccaca aaatgcggga tagcgccgca
     6121 ataggtcacg gcattggctg ttgcaacgaa cgtaagatcc ggaacgagca cttcatcgcc
     6181 cgattcgaca cccgccattt tcagagatat atggagcgcc gcggtaccat tcacgaccgc
     6241 aaacgcgcgt ttagcaccgg tatattccgc cagcttctgc tcaaaagtat ccacaaactt
     6301 cccgaccgag gatacccagt ttgtatcaag gcactctttg acatatttcc attcattccc
     6361 gcagaaatac ggctcgtgaa gttcaatatc cctccggcct tctggcaaac aggagcgaat
     6421 cgacttcaac acagcatctt tattgagttt cgaatttaca tgttgcataa atgggattta
     6481 tattgggcca gatgcccgct ctctttgaac catttgatcg tttcagtcaa acctcgtttg
     6541 aagccggcaa atccggcata cgcgggcatc cagccggtca gcttcctggc tttcgtatga
     6601 tcagcccaaa gccgttccac ctcgctcttg gccggtcgaa tgcgcgcatt atccttcgtc
     6661 atcttgatct tcgagttcat gatctccgcg atcgtccggg caacatcgcc gatcgagatc
     6721 tcgaaattac ttccaaa
//
LOCUS       DAOSIO010000080         6726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_10304_length_6726_cov_4.559886, whole genome shotgun sequence.
ACCESSION   DAOSIO010000080 DAOSIO010000000
VERSION     DAOSIO010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10304_length_6726_cov_4.559886"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PK590_04115"
     CDS             complement(<1..270)
                     /locus_tag="PK590_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23814.1"
                     /translation="MVTRKVFYFLAVIMLSLPPFALAIEGPEPSGSAEDTGGEASGNG
                     KTVACCVGQPTMVAADDGGVVVLAGKKLKKYDNALNLVKEVSVVDA"
     gene            complement(303..1310)
                     /locus_tag="PK590_04120"
     CDS             complement(303..1310)
                     /locus_tag="PK590_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOG23815.1"
                     /translation="MATYKELGFVNTREMFANAMKGRYAVPAYNFNNMEQIQAIVVAC
                     VETQSPVILQVSSGARKYANQTLLEYMGKGAVEMMKAYAKEKGLKPIPMALHLDHGDT
                     FELAKSCIESGFSSVMIDGSHHPFEKNIELTRQVVELAHKRDVTVEGELGVLAGIEDE
                     VSAEHSTYTDPNDVVKFVGETGVDSLAISIGTSHGANKFKPEQCTRNAEGILIPPPLR
                     FDILKEIEQKIPGFPIVLHGSSSVPQEAVKIINENGGALKDSIGIPEEQLRRAAQSAV
                     CKVNIDSDGRLWMTAAVRKIFKEKPAEFDPRKYLGPARDALIAMYKHKNENVLGSAGK
                     A"
     gene            complement(1353..1940)
                     /locus_tag="PK590_04125"
     CDS             complement(1353..1940)
                     /locus_tag="PK590_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003367634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOG23816.1"
                     /translation="MKDLKGTRTSENLMKSFAGESQARMRYTYYASTAMKEGYRQISN
                     IFIETADNEKEHAKLFYKHLLAGGLQDAVQEIHASYPIAFADTLKNLEAAANGEKEEW
                     SDLYPAFAEIADQEGFPEVARTFRHVSMVEKRHETRYRKLHDALAQHKLFVKDGKVFW
                     KCLECGYIVEASRAPERCPVCDHPKGHFEIWIENY"
     gene            complement(2119..3282)
                     /gene="dprA"
                     /locus_tag="PK590_04130"
     CDS             complement(2119..3282)
                     /gene="dprA"
                     /locus_tag="PK590_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOG23817.1"
                     /translation="MNSLSEKDWMALLLLNRLGVEDRRRARNLFAEGHRPERVVELLD
                     PRSVAAHITFDPQKELSLCWQKGVSLCSILEDSYPVSLREIADPPLVLYMKGRFELND
                     ENALAVVGTRHPSFYGRTQAKRFAGELASKGVTIVSGLARGIDQAAHEAALDVPYGRT
                     IAVLGCGIDIVYPKEHVKLFERISQKGVLISEYAIGTPPLAENFPRRNRIISGLGHGV
                     LVVEAHSRSGSLITAHEALDQGREVFSIPGPVDQLTSRGTHQLIKEGAVLAESPEEVF
                     EALSGKWKVFSTEMSAPVSNASAVCPFKEVVDEKKLPPKTQNPEGETPLLTAGENLNC
                     VVEALNEEGSLMTDEILDITGLDPGELPGILLKLELDGKIRRRRDGKYETGSS"
     gene            complement(3433..4530)
                     /gene="aroB"
                     /locus_tag="PK590_04135"
     CDS             complement(3433..4530)
                     /gene="aroB"
                     /locus_tag="PK590_04135"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOG23818.1"
                     /translation="MKIISVDSQKRKYKVVIGKGLLGKLGQFVKGIFHPRARVMIVFQ
                     TGVSVYARSAASSLKRNGYTVYFHKVLDGEKAKSQANLFRLLRDLLAKGFERTDGILA
                     IGGGVVSDLSGFAASVYLRGIPYVNVATTLLAQVDSSIGGKTGINLPEGKNMMGSFYP
                     PALVISDMDVLRSLPERELRASLAEVVKYGVIRDPGLFSFLESNTSKILNKNPAALER
                     IVTDSARIKAEVVSRDEFETRGERIILNYGHTFGHGIENAARYKKIVHGEAVAIGMAM
                     AAHLAAELGICSPGLVKRQMDLLKTFGLPVSVDALKINATTSFQAMMRDKKKAHGQLR
                     FVLPVRIGEVVIRAGISAGEIRSILRLFGAK"
     gene            complement(4540..5046)
                     /locus_tag="PK590_04140"
     CDS             complement(4540..5046)
                     /locus_tag="PK590_04140"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOG23819.1"
                     /translation="MPNLYLAGMMGAGKSVTGRALAALMSYGFVDLDSEIEKKEGRPI
                     TAIFSSEGEPYFRDVETRVLKEFSLKERRVVSTGGGIVLRDENVEQMRRTGKVIFLEA
                     SLPVLWERVCQNKNRPLLNTPDPKGSLERILKGREKRYKATCDFSVMTDGLSPESVAR
                     QIMGHLKQ"
     gene            complement(5039..6157)
                     /gene="aroC"
                     /locus_tag="PK590_04145"
     CDS             complement(5039..6157)
                     /gene="aroC"
                     /locus_tag="PK590_04145"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOG23820.1"
                     /translation="MLNYITTGESHGRYMAVILEGMPSGLGIDMDYINGELRRRQGGF
                     GRGGRQKIEADYVEIMGGVIRKKTTGAPIGLLLKNKDHTVDVLPSIERPRPGHADLAG
                     SLKYHQDIRAVLERSSARETAMRVAVGAVCKLFLKEFGIDIAGHVTRIGPVAIPKEAL
                     SVNEIRRLTKGSLVNCVCRKTEQAMIRLIEKAQKQKDTLGGKYEIRAEGLPIGLGSYV
                     HYERKIDAQLAQILMSQQSAKAVEIGAGDQLASLWGSEAHDEIFYSKTRGYYHKTNRA
                     GGIEGGMTNGEAVVVKVTMKPIATLARPLSSVNMKTRKAEKADFERSDFCAVPAGSVI
                     GESLVAYVLARSFLEKFGGDSMREIRRNYDGYLRQVRA"
     gene            complement(6161..>6726)
                     /locus_tag="PK590_04150"
     CDS             complement(6161..>6726)
                     /locus_tag="PK590_04150"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HOG23821.1"
                     /translation="RQIRSQLTAVSWLLIWRMYGLSPEFAIAVVFVSAMIVVTLTDLE
                     TGLIPDLITIPGMLFGLVVSVVNTDAFPEGLWYHKLFASAIGLAGGGAVLMLTGWLGN
                     LVFRKESMGGGDIKLLAMTGAFIGIEKTLLVFMFSPFIALPFAIFYRFVKRQETIPYG
                     PFLALMSVVFFFKGTEILNFLSTLYGV"
BASE COUNT         1684 a         1919 c         1493 g         1630 t
ORIGIN      
        1 ggcatcaacc acggagactt ctttgacaag attcagggca ttatcatatt ttttaagttt
       61 cttgcccgcc agcacgacca cacctccatc gtccgccgca accatggtag gctgtcctac
      121 gcaacatgcc accgtctttc cgttaccgct ggcttctccc cccgtatcct ccgcgctccc
      181 cgaaggctct ggcccttcta tcgccaaagc aaaaggcggc aaactcaaca taataaccgc
      241 aagaaaatag aagactttcc gcgtcaccat acccgttaaa gaagtccgtc gcctgaacaa
      301 acttaagcct tgcccgcgga accaaggaca ttctcgtttt tgtgtttata catcgcgatc
      361 agcgcgtccc gggccgggcc aaggtatttg cgtggatcaa attccgcggg tttttctttg
      421 aaaattttac gaacggcggc cgtcatccaa agccggccgt cggaatcaat attgaccttg
      481 caaacagcgg actgagcggc ccgccggagt tgctcctcag gaatgccgat agaatccttt
      541 aacgcgccac cgttctcatt gataattttc accgcctcct ggggaacgga cgaagacccg
      601 tgaagcacaa tcggaaagcc gggaatcttc tgctcaattt ccttcaggat atcaaatcgg
      661 agcgggggcg gaatgagaat tccttccgca ttcctcgtac actgttccgg cttaaacttg
      721 ttggccccgt gtgaagtccc gatcgaaatc gcaagggagt ccacgccggt ttccccaaca
      781 aatttgacca catcattcgg gtccgtgtag gttgagtgct cagcactcac ttcatcttca
      841 atcccggcca ggacgcccag ctcgccttcc accgtcacat cacgcttgtg ggccagctca
      901 accacctggc gagttaactc aatattcttc tcaaatggat gatgggatcc atctatcatg
      961 accgaagaaa agccggattc gatgcaagac ttggcaagtt caaacgtgtc cccatggtcc
     1021 agatggagag ccatcgggat aggcttaagc cccttttctt tggcgtaagc cttcatcatt
     1081 tccaccgccc ctttccccat gtactcgagg agcgtttgat tggcgtactt tcgcgcgccg
     1141 cttgaaacct ggagaatcac gggagattgc gtttcgacgc acgccaccac aatggcctgg
     1201 atttgctcca tgttgttgaa gttataagcc gggacggcgt acctcccctt catggcgttg
     1261 gcgaacattt cgcgcgtatt aacaaaacca agctccttgt aagtagccat ttcattactc
     1321 cttttctaat ttaaaatttc ggatgttaaa aatcagtaat tctcgatcca gatttcgaaa
     1381 tgtcccttcg gatgatcgca caccggacaa cgctctggcg cccttgaggc ttccacgata
     1441 tatccacatt ccagacattt ccagaacacc ttgccgtcct tgacaaaaag tttatgttgc
     1501 gccaaggcat catgaagttt ccggtagcgg gtctcatggc gtttttcgac cattgatacg
     1561 tgtctgaacg tacgggccac ttccgggaat ccttcctggt ccgctatttc cgcgaacgcg
     1621 ggataaagat ccgaccattc ttccttttct ccattcgcgg cggcttcaag attcttgaga
     1681 gtatccgcga acgcgatcgg atatgaagcg tgaatttcct gaaccgcgtc ctgcaaacca
     1741 cccgccagga gatgtttata aaacaatttc gcgtgctctt tttcgttatc cgcggtctct
     1801 ataaaaatat tggaaatctg ccgatatcct tccttcatgg ccgtggaggc atagtaggtg
     1861 taacgcatcc gcgcctggga ttcgccggca aaagatttca taagattttc cgaggttcgt
     1921 gttcctttca agtccttcat aaaatttttc tcctggttac attatgttaa cgggaaatga
     1981 acgaaagatt cagatgttgg actgagtaaa ccggaagaat tatatcaaac ccctttcaaa
     2041 tgtaaactat cgatggctgg acatcagagg cccgaaatca tctcgccatg ccagggggga
     2101 ggttgggaaa acgggtgatc aggagctccc ggtttcatac tttccgtcac gccgccggcg
     2161 aattttcccg tcaagttcaa gctttaagag tattccgggt agctcccccg gatcaagccc
     2221 cgtgatatca agaatttcat ccgtcatcaa acttccctct tcgttcagcg cctcaaccac
     2281 gcaattcaaa ttctctccgg ccgtcaggag aggcgtttcc ccttcgggat tttgagtttt
     2341 aggcggaagt tttttttcat ccacaacctc cttgaacggg caaacagcgg acgcgttaga
     2401 aacgggagca gacatctcgg ttgaaaaaac tttccacttt cctgaaaggg cctcgaaaac
     2461 ttcttccgga ctctccgcga gaacggcgcc ttccttgatt aactgatgag tcccacgcga
     2521 ggtcaattga tccacaggcc cgggaatcga aaacacttcc cgcccctgat ccagcgcttc
     2581 atgggccgta atcagggatc cgctccgcga gtgagcctcc accacaagaa ctccgtgacc
     2641 gaggccggaa ataatccggt tgcgcctggg aaagttttcc gcgagaggcg gtgtcccgat
     2701 cgcgtattca cttatcagga cgcctttttg tgaaatcctc tcaaacaatt tcacatgttc
     2761 tttgggataa acaatgtcga tcccacaacc cagcaccgcg atcgtcctcc catacggcac
     2821 atcaagagcg gcttcatgcg ccgcctgatc aataccgcgc gccagccccg aaacgatcgt
     2881 gactcctttg gaggccaact cccccgcgaa tcttttagcc tgtgttcggc cataaaaact
     2941 cgggtgacgg gttccaacaa ccgctaaagc gttctcatca ttgagctcaa atcgtccttt
     3001 catgtagaga accaggggcg gatcagcaat ttccctaagc gagaccggat aagagtcctc
     3061 aagtattgaa caaagtgaaa cccccttctg ccagcataag gaaagttctt tttgtggatc
     3121 aaaagtaatg tgagcggcaa cactccgggg atccagtaac tcgaccaccc gctccggacg
     3181 atgcccttcc gcaaaaagat ttcgcgcccg gcggcgatct tcaacgccaa gacggttcag
     3241 aaggagcaag gccatccagt ctttttcaga aagcgaattc ataagagacc aagcttcgaa
     3301 ggacgtgcgt cgagagtgta tatgtcgcaa tttttcatga ggagaggata tgataaaatt
     3361 gcttataaat caatatatat ttcgctttac gtcaacatcg cgacacataa actctcttgg
     3421 gaggaagtgg atttattttg cgccaaagag gcgaagaata ctgcggattt cgccggcaga
     3481 aattcccgca cggatcacaa cttcaccgat tcgaacggga agaacgaagc ggagctggcc
     3541 atgagctttt ttcttatctc tcatcatggc ctgaaaactg gtggtagcat tgatcttcaa
     3601 cgcatcgact gaaaccggca aaccaaaggt cttgagcaaa tccatttgcc gtttcacaag
     3661 gcccggggaa caaataccaa gctcagccgc aagatgggcc gccatcgcca ttccgatcgc
     3721 gacagcttcc ccatggacaa tttttttata cctcgcggca ttttcaattc cgtgaccaaa
     3781 agtatgcccg tagttaagaa tgatccgttc cccacgggtt tcaaactcat cgcggctgac
     3841 aacttccgcc ttgatccgcg ccgaatcggt cacgatcctt tccagagcgg ccggattttt
     3901 attaagaatt ttggaggtat tcgattcaag aaaactgaaa aggcctggat cgcggatcac
     3961 tccatacttc acaacttccg caagcgatgc tctgagttcg cgttcaggga gcgacctcag
     4021 tacgtccatg tctgaaatca cgagggcggg aggataaaat gaacccatca tgtttttccc
     4081 ctcgggcaag ttaatccccg tcttcccgcc aatcgagctg tcgacctgcg ccagaagcgt
     4141 cgtcgcgaca ttaacataag ggatcccgcg caaatacacg gaggcggcaa accctgaaag
     4201 atcacttaca actccccctc ctatcgccaa aatcccgtcc gttcgttcaa aacccttggc
     4261 aagaagatcg cgaagcaacc ggaaaagatt cgcctgcgac ttggctttct ctccatccaa
     4321 aaccttgtga aaataaacag tatatccgtt tctctttaaa gagctcgcgg cggaacgcgc
     4381 gtagaccgaa actccggttt gaaaaacaat catcactcga gccctgggat gaaaaatccc
     4441 cttaacaaac tggccaagct tccccaggag ccccttgccg attacgacct tgtactttct
     4501 cttttgacta tcaacggaaa tgattttcat tctgccttct tactgtttta aatgccccat
     4561 gatctgccgg gccaccgatt caggagacaa accatccgtc atcacggaaa aatcacacgt
     4621 ggccttatac ctcttctccc gtcctttcag aattctttcg agcgatccct tcgggtccgg
     4681 agtattaaga agcggccggt ttttattctg gcaaaccctc tcccacaaaa ccggaaggga
     4741 cgcctccaga aaaatcacct tccctgtcct tcgcatctgt tcaacatttt cgtcccgaag
     4801 aacaattccg ccgcccgttg aaacaacccg tctctctttc agagagaatt ccttaagcac
     4861 gcgggtttcc acatcgcgga aatacggttc tccttcggaa gaaaaaatcg cggtgattgg
     4921 acgaccttct tttttttcaa tttccgaatc aagatctaca aaaccatagc tcatcagagc
     4981 ggccaacgcc cttccggtaa cgcttttccc ggcgcccatc atgccggcaa ggtacagatt
     5041 aggcacgaac ttgcctcaaa taaccgtcgt aattacgccg gatttctctc atcgagtctc
     5101 cgccaaattt ctcaagaaac gagcgagcga gcacataagc cacaagggac tccccgataa
     5161 cacttcccgc tgggaccgcg caaaaatcac tcctctcaaa gtcagctttc tcagctttcc
     5221 gggttttcat gttcacggag cttaagggtc ttgcgagagt cgcgatgggc ttcatagtaa
     5281 ctttcacgac cacagcttct ccgttggtca taccaccttc gatcccgccc gcgcgattgg
     5341 tcttgtgata ataacctcgg gtcttggaat aaaaaatctc atcatgagct tcagaacccc
     5401 agagagacgc cagttgatca ccggcgccga tctcaaccgc tttggcagac tgctggctca
     5461 tcaggatttg cgcaagttgg gcgtcaattt ttcgctcgta gtgcacataa gaacccaacc
     5521 cgatcggcag gccttccgct cgtatttcat atttgccgcc aagggtatct ttctgtttct
     5581 gggctttttc aatcagccgg atcatcgctt gttctgtttt tctgcagaca cagttcacca
     5641 gagagccttt ggtgagcctg cgaatttcgt tcacagagag cgcttctttg gggatcgcca
     5701 ccggcccgat ccgggtcaca tgtcccgcga tgtcgattcc aaattccttc agaaaaagtt
     5761 tacagaccgc tcccacggca acccgcatcg cggtttcccg cgcgcttgaa cgttccagaa
     5821 ccgcgcgaat gtcctgatgg tatttgaggg atcccgcaag atccgcgtga cccggccgcg
     5881 gccgctcaat ggaagggaga acgtctaccg tatggtcttt atttttcagg agtaatccta
     5941 tcggggctcc ggttgttttt ttccggatga ctccccccat aatttcgaca tagtccgcct
     6001 cgattttttg tcttccgcca cgcccaaacc ctccctggcg acggcgcaat tcgccgttaa
     6061 tataatccat atcaatccca agtccgcttg gcataccttc cagaattaca gccatatacc
     6121 ttccgtgaga ttcgccggtc gtgatatagt tcagcataaa tcagactccg tatagtgttg
     6181 acaaaaaatt caggatttcc gtacctttaa aaaagaagac gaccgacata agagccaaaa
     6241 aagggccgta cggtatggtc tcttgtcttt tcacaaaacg ataaaaaatc gcgaaaggaa
     6301 gcgcaataaa cggggaaaac atgaaaacca gcaacgtctt ctcaatcccg ataaacgccc
     6361 cggtcatcgc gaggagcttg atgtctccgc ctcccatact ttccttgcga aacacgagat
     6421 ttccaagcca gcccgtaagc atcagaacag cgccccctcc ggccaaacca atcgcgctcg
     6481 cgaacagttt atgataccac aagccctccg gaaaggcgtc cgtgttcacg acactcacca
     6541 cgagaccaaa aagcatgccg gggattgtaa ttaaatcggg aatgaggccc gtctccaaat
     6601 cagtcagcgt caccacaatc atcgctgaaa caaaaacaac ggcgatcgca aactccggtg
     6661 agagtccata cattcgccaa atcagaagcc agctgacggc cgtcagctgg cttctgattt
     6721 ggcgaa
//
LOCUS       DAOSIO010000081         6689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_10382_length_6689_cov_4.201839, whole genome shotgun sequence.
ACCESSION   DAOSIO010000081 DAOSIO010000000
VERSION     DAOSIO010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6689
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10382_length_6689_cov_4.201839"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="PK590_04155"
     CDS             <1..1023
                     /locus_tag="PK590_04155"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOG23822.1"
                     /translation="TRRLGKTGLEVSEIGFGCWAIGGNAYGKVDDRESLEALEAAWSG
                     GVNFFDTADTYGEGHSESLLAEFLKGKPRDQVLIATKAGWDFYPEDLKQRRAIADSES
                     RNFAGHRKNFDGDYLRFACEQSLNRLGVDHIDLYQLHNPTLGQIHSGDPVSTLETLKR
                     EGKIRAIGISVHREEEALAAMEDPRVESLQVIFNVLDQRMAECVFAESEKKNVGVIAR
                     EPLASGLLTGKYPPGHEFPKTDHRRRWSPEKREWDWQKIQVIRHVLGNPLVTGDPGRA
                     AQAALEFVLSFPAIASVIPGAKTKTQALNHQGAVSAPFLSESDKAQLRALWMDNPLFR
                     KGLLPR"
     gene            1020..2282
                     /locus_tag="PK590_04160"
     CDS             1020..2282
                     /locus_tag="PK590_04160"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HOG23823.1"
                     /translation="MKILRMIFLWLMIMAALVYFHDHWVGLSMQPVLEHQLADAFDMP
                     FNIDGLKISLFPPGRITLKKIECLNPREFRRPQHFVGEGVTLFIDLPALKRKCIRIKK
                     AHFKKFLFAVETHMNGKESRSNTFIWYHHMGLDEDDDPSMNFPKQDSPSSEQAAAEAE
                     ARGKWRVMIDRLELDDGTFVYDDLRLPKDERRWIFQNLKGFWEGFDLIPGYTSPEFRE
                     YIELEGTFGQNPPARFAGKGKCQFSDGNNFDLELSIEEGSIAEYKFLLDGLPGEVTGG
                     RFDLRSRGICHESNLRSAHVLTLREMKFIAPTLRQKILKYPFGTVLLALESQKKIELE
                     IDVNGYIGDPKFRFFSAFTKAFRKSLTQKAKAVLNEIKKGTTMVATEAPSHVRDGVGQ
                     IGNLLNNTFSTLNPLTVLGENQRKKDEK"
     gene            2272..3192
                     /gene="era"
                     /locus_tag="PK590_04165"
     CDS             2272..3192
                     /gene="era"
                     /locus_tag="PK590_04165"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOG23824.1"
                     /translation="MKNNSETRAGYVSIIGRPNVGKSTLLNFLVGEKLAGVSSKPQTT
                     RDVVRGIVTIPNGKKVGSPYRGQIVFLDTPGVHEPHDQLGKEMLHGARHSLDDADVLF
                     WMVYPREIGDTEERMLGWLKDFRKPVFLLVNQIDKFPRLQVLPAIDSYQKVYPFKDFI
                     PISAVTGENIDVLMQKTLECLPKGDFIYPEDQISDQNERFHVRELIREKLYHELEDEL
                     PYDATVRIESYKDEEKITRISATIIVSRESQKAIVIGKGGLKIREIGRTARLEIENLI
                     GRKVFLELWVKVIAGWKENASLLGEIREGS"
     gene            3207..4250
                     /locus_tag="PK590_04170"
     CDS             3207..4250
                     /locus_tag="PK590_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HOG23825.1"
                     /translation="MKILVFGAGAIGSAFGGFLSGRHDVTLLGRKPHIDAIRRRGLKV
                     TGIWGRHTFRFRELVTDIRSLDRSQPFDLILCAVKSHGTSRAAGLIQKIMTPSTCVVS
                     LQNGLGNIESLCEKIPVKQVLAGRVIFGVELHGPGRIHVSVIAQPTAIGEITGGRVTQ
                     RVKMIVRALNASKLPSVATPDVRGLLWLKVIYNAALNPLASLMRSHYGKLGENEGARE
                     TMNAVIDEIYQVARKAKVRLDPSTAGAYKKIFYAKLLPRTYDHHPSMLQDLQKGKRTE
                     IDSLNGRIVKLGRQHGVPVPVNACLTRAIKRMEKHAPQLEKYPKMRPRKMFAQETMRA
                     ILKPEWKKKQLTP"
     gene            4360..5601
                     /locus_tag="PK590_04175"
     CDS             4360..5601
                     /locus_tag="PK590_04175"
                     /inference="COORDINATES: protein motif:HMM:NF026247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HOG23826.1"
                     /translation="MQPVENAKISNEMRASRKDNKETLAIFFIQPFIAFLCAISQLRN
                     KYSFAVIFLFFVLFGFTFLAQNESFDSFRYVQQFYQYIPYDLAQYQHDLADFFTLKSK
                     VKDLYVISSYFIVSKFTSNYHVLMALWAMVFSFFCLKSFRFFVDRPEFGKSLAVFLLA
                     FLFLSSNSIFNINGVRFWTAAWVAVYVVFEIMVNGNYKFIALSLLTPLIHISYLFFVG
                     VLIVFLLARKFENVWILLFFVSFFVSEISIRLFQNYQEHLPLVIQNMIWSYAAEENLA
                     EHQRILERIPLYAKILNSLPRYFINLLMVVFIGYYKRMWLFKNGRVIFVFLLIWLSFV
                     NFTMSILSFGSRFIVLSVPFVCYLGLLTYKHIPMLPKLIYLIPVVYSYSILYWCRAMI
                     SVTDPCLIISVFPHIIIKNLL"
     gene            5601..>6689
                     /locus_tag="PK590_04180"
     CDS             5601..>6689
                     /locus_tag="PK590_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23827.1"
                     /translation="MKKLNILIISRNILPKNSPRAFRATELATELARQGHSITLYSIL
                     GSYDYSKYEEETGVKVRNMGKMLFATHDSDGDFRYTLLDKILYHSLHRLIEFPDIELM
                     FRVPCIIRKEREMDLLITIASPHAIHWGGALARTLIPDSKFPKIWVADCGDPYMGNSI
                     ENNKFSYFKYVEKWWGSKADYITIPLEEGKEGYYREFHSKIKVIPQGFDFSKVKLAKP
                     YVRNVIPSFAYAGTIYLGKRDPKSLLDFLILIERDFRFVIYTNTPDYYSGYKKKLKEK
                     LAIKPYVPRDRLIFELSQMDFLINLKNPDRRQSPSKIIDYLLSQRPIVNISSDFPEHE
                     RNVFLEFIHGDYTHRLKNSNIDQYNIQNV"
BASE COUNT         1745 a         1506 c         1650 g         1788 t
ORIGIN      
        1 acgcgccggc tcggcaagac aggccttgag gtttctgaaa tcgggtttgg ctgctgggcg
       61 atcggcggta atgcctacgg aaaagtcgac gatcgcgaat ctctcgaagc gcttgaggcg
      121 gcctggtccg gaggtgttaa tttttttgat acggccgaca cctacggtga agggcatagt
      181 gaatccctcc tcgctgaatt cctgaaaggc aaacccagag accaggtcct gattgccacc
      241 aaagctggtt gggactttta tccggaagat ctgaaacagc gtcgcgcgat cgcggattca
      301 gaatcccgca actttgccgg ccaccggaaa aatttcgatg gcgactatct tcggttcgcc
      361 tgtgaacaaa gtttaaatcg tctcggagtg gatcatatcg acctttatca attgcataat
      421 ccgacccttg gacagatcca ctccggcgat cccgtttcga cgcttgaaac gctgaagcgg
      481 gaagggaaaa tcagggcgat tggaatctcg gtccaccgtg aagaagaggc gcttgccgcg
      541 atggaggatc cgcgcgtcga gagtctgcaa gttattttta atgtcctcga ccaacggatg
      601 gcggaatgtg ttttcgcgga atcggaaaaa aagaacgtgg gggtgatcgc ccgcgaaccg
      661 ctggcgagtg gcctgctgac ggggaaatat ccccccggtc atgaattccc gaaaacggat
      721 caccgaaggc ggtggtctcc cgaaaaaagg gaatgggact ggcaaaaaat ccaggtcatc
      781 cgtcatgttc tgggaaatcc cctggtgacc ggggatccgg gacgggcggc gcaggcggcg
      841 ttggaatttg ttctgtcatt tccggcgatt gcttccgtca tcccgggcgc caaaacaaaa
      901 acccaggccc tgaatcatca aggcgcggtc tccgccccct ttctttcgga aagcgacaaa
      961 gcccagctcc gggcgctctg gatggataat ccgctttttc ggaaaggatt acttccccga
     1021 tgaaaattct gagaatgatt tttttgtggc tgatgatcat ggccgcgctt gtttattttc
     1081 atgatcactg ggtcggtctg tcgatgcaac ccgtgcttga acatcagctg gccgacgctt
     1141 tcgacatgcc cttcaatatt gacggtctca agattagttt attccctccc ggaagaatca
     1201 cgctcaaaaa gatcgaatgt ctgaatccca gggaattcag gcggccccag cattttgtgg
     1261 gagaaggggt gacgcttttc attgatcttc ccgcgctaaa acggaaatgt atccggatta
     1321 aaaaagcgca tttcaaaaaa tttcttttcg cggtcgagac acacatgaac ggcaaggaat
     1381 cgcgcagtaa cacgtttatc tggtatcacc atatgggact tgacgaagac gacgatccct
     1441 cgatgaattt tccgaaacaa gattcgcctt cctcggaaca ggcggcggcg gaggccgagg
     1501 cccgggggaa atggcgggtg atgattgacc ggctagaact cgacgacggg acctttgttt
     1561 acgatgacct gcgcctgccg aaagacgagc ggcgatggat cttccagaac ctgaaaggtt
     1621 tttgggaggg ttttgattta attccgggat acacaagccc cgagttccgg gaatacattg
     1681 aactggaagg aactttcgga caaaatcctc ccgcccggtt cgcggggaaa ggaaaatgtc
     1741 agttttccga cggcaacaat ttcgacctgg agctttcgat cgaagaaggt tccattgccg
     1801 aatataaatt tttgctggac gggcttcccg gtgaggtgac cgggggcagg tttgatctgc
     1861 ggtcccgggg aatttgtcac gagagcaacc tgaggtccgc ccatgtcttg acgttgcggg
     1921 agatgaaatt tatcgctccc accttgcggc agaaaatttt gaaatatccc ttcggcacgg
     1981 tgcttctggc gctcgaaagc cagaaaaaaa ttgaactgga aatcgacgtt aacggctata
     2041 tcggagatcc caaattccgt tttttcagcg cttttaccaa agccttccgg aaatccctga
     2101 cgcaaaaagc caaagcggtc ctgaatgaaa ttaaaaaggg gaccaccatg gtcgcgaccg
     2161 aagcgccttc ccatgtccgg gatggtgtgg ggcaaatcgg aaatcttctc aataacactt
     2221 ttagtaccct gaacccgctg acggttctgg gggaaaacca gaggaaaaaa gatgaaaaat
     2281 aattctgaaa ccagggccgg ctatgtctcc attatcgggc ggccgaatgt cgggaaatcc
     2341 actctcctga attttctcgt gggtgagaag ctcgcgggcg tttcttcaaa accgcagacc
     2401 acgcgcgatg tcgtccgagg gattgtcacg attccgaacg ggaaaaaagt gggtagccct
     2461 tatcgcggac agatcgtctt tcttgacacg cccggtgtcc acgagcctca cgatcagctg
     2521 ggaaaagaaa tgcttcatgg ggcgcgtcat tcgctcgatg acgcggacgt gcttttctgg
     2581 atggtttacc cgcgggagat cggagacacg gaagaacgga tgcttggctg gctgaaggat
     2641 ttcaggaagc ctgtttttct gctcgtcaac cagattgata aatttccgag gcttcaggtg
     2701 cttcccgcga ttgattctta ccagaaggtg taccctttca aggatttcat tccgatcagc
     2761 gcggtgaccg gagaaaacat tgatgtcctg atgcaaaaaa cgctcgaatg tcttccgaag
     2821 ggtgatttta tctatcccga agaccagatt tcggaccaaa acgagagatt ccatgtccgg
     2881 gaactgatac gcgaaaaact ctatcatgag cttgaagatg aactccctta tgatgccacg
     2941 gtccggatcg aaagttacaa ggatgaggaa aaaataaccc ggatcagcgc gaccattatc
     3001 gtcagccgcg agtcgcagaa ggcgattgtg attggaaagg gcgggctgaa gatcagggaa
     3061 atcggccgta cggcgcgtct tgaaatagaa aacctgatcg ggcggaaagt ttttctcgag
     3121 ctctgggtga aggtcattgc cggctggaaa gagaatgcct ccctgctggg cgagatccgc
     3181 gaaggatcct gaagaagaga gcgaagatga agatacttgt ttttggggcg ggcgcgattg
     3241 gttcggcgtt cggcggattt ctttccgggc ggcatgatgt gaccttgctt gggagaaagc
     3301 cccacattga cgcgatccgg agacgcggtc tcaaagtgac gggaatctgg ggccggcata
     3361 cgttccggtt cagggaactt gtgaccgaca ttcgaagcct tgaccgatcc caaccgtttg
     3421 atcttatcct gtgcgcggtc aaatcccacg gcacgtcgcg cgcggcgggc cttattcaaa
     3481 agatcatgac tccttcgact tgtgtcgtct ccttgcaaaa tggtctcgga aatatcgaat
     3541 cgctttgtga gaaaattccg gtcaaacagg tgcttgcggg gcgggtgatt tttggtgttg
     3601 agctccacgg gccgggcagg attcatgttt cggtaatcgc ccagccgacc gcgattggtg
     3661 agatcaccgg cgggcgcgtg acccagcgcg tgaaaatgat cgttagagcg ttgaacgcgt
     3721 caaagcttcc ttcggtggcg acccctgacg tcagaggact gctttggctc aaggtcatct
     3781 acaatgccgc tcttaatccg ttggcctctt taatgcggtc ccattacggg aaattgggag
     3841 agaatgaagg ggcgcgggag accatgaacg cggtgattga tgaaatttat caagtggccc
     3901 gaaaagccaa ggtccggctc gatccttcca cggccggcgc gtacaagaaa atattttacg
     3961 ccaaacttct cccccgaact tatgatcatc atccatccat gctccaggat ctccagaaag
     4021 ggaaacgcac ggaaattgat tcgttgaacg gaaggatcgt aaagcttggc cgccagcacg
     4081 gtgtccctgt tcccgtcaac gcttgcctca cgcgcgccat caagcgaatg gaaaaacacg
     4141 ccccccagct tgagaagtac cccaaaatgc gtccccggaa gatgtttgcg caggagacaa
     4201 tgagggccat cttaaaacca gaatggaaaa agaaacaatt gaccccttaa agacgaaaac
     4261 catacaatct caatcgcgcc cgaatcaagg ttaaattgca aacttaaatt ctgacaccga
     4321 tcctatggat tacatagatg acgtaaaaga ttgattaaaa tgcaacccgt tgaaaatgcg
     4381 aagattagca atgaaatgcg cgccagtaga aaggacaaca aagaaactct ggctattttt
     4441 ttcatccagc cgtttattgc tttcctgtgt gcgataagtc agttgagaaa caagtattca
     4501 ttcgcggtaa tttttttgtt ttttgtgctt tttggattta cgtttcttgc ccaaaatgaa
     4561 agttttgact cattccgcta cgtccagcaa ttttatcaat atatcccgta tgatttagct
     4621 caatatcagc atgacctcgc ggatttcttt acgcttaaat caaaagttaa agatctgtac
     4681 gttatttcaa gctattttat tgtttcaaaa ttcaccagca actaccatgt gcttatggct
     4741 ctttgggcaa tggtattttc tttcttttgt ctgaaatctt ttcgtttttt tgttgatcga
     4801 cctgagtttg ggaagtctct tgccgtgttt ttgctcgcgt ttttattcct ttccagcaat
     4861 agcatattca acatcaatgg ggtccgattc tggacagcgg cttgggtggc ggtctatgtt
     4921 gtttttgaaa ttatggtaaa cgggaattac aaatttatag cactgtcact ccttacaccg
     4981 cttatccata tctcgtattt atttttcgtt ggagttctca ttgttttttt actggccaga
     5041 aagtttgaga atgtatggat acttttattt tttgtcagtt tttttgtgtc agaaatatct
     5101 atccgattat tccaaaatta tcaggaacat ttgcctttag ttattcagaa tatgatctgg
     5161 tcttatgccg cagaagagaa tttggcggaa catcaacgta ttcttgagcg aattccattg
     5221 tacgcgaaaa tcctgaacag tttgccgcgt tattttatta atttactgat ggttgttttt
     5281 atcggctatt acaaaagaat gtggttgttc aaaaatgggc gtgttatttt tgttttcttg
     5341 ttaatttggt tatcatttgt aaattttacg atgtcaatcc tcagttttgg tagcagattt
     5401 attgtattat ccgttccgtt tgtctgttat ctggggttac tcacttacaa gcacattccc
     5461 atgctcccca aattgattta tcttataccg gtcgtgtaca gttactcgat cctctattgg
     5521 tgtagagcaa tgatttcagt gactgaccca tgtttaataa tctccgtatt cccacacatc
     5581 atcatcaaaa atttactctg atgaaaaaat taaacattct gattatctca aggaacattc
     5641 tcccgaagaa ttcgcccaga gcatttcggg cgacagagct tgctactgaa ttggcgcgtc
     5701 aggggcattc aattaccctg tattcgattt taggtagcta tgattactct aaatatgaag
     5761 aagaaaccgg agtcaaagtc agaaatatgg ggaagatgct gttcgctact catgatagtg
     5821 atggtgattt ccgctatacg ttactggata aaattcttta tcattccctg catcgcctta
     5881 tcgaatttcc tgatattgag ctcatgttta gagtcccctg catcatcagg aaggaaagag
     5941 agatggatct gctgattaca attgcatctc cgcacgcaat acactgggga ggagcgctcg
     6001 ccagaaccct gatccccgat tctaaatttc caaaaatctg ggttgctgat tgcggggatc
     6061 cctatatggg caattccata gaaaataaca aattttcata tttcaagtat gtcgaaaaat
     6121 ggtgggggag caaagcggat tatattacca tccctcttga ggaaggaaag gagggatatt
     6181 accgggaatt tcactcaaag attaaagtca ttcctcaagg ttttgatttt agtaaagtga
     6241 agttggcaaa gccctatgtg cgtaatgtca tcccttcatt cgcgtatgct ggaactattt
     6301 atcttggaaa gagagatccg aaatcattgc ttgatttttt gattttaatc gaacgagact
     6361 ttagatttgt tatttataca aatactcccg attactacag tggttacaaa aaaaaattga
     6421 aagaaaaatt ggcgattaaa ccctatgttc cccgggatcg gttgattttt gaattaagtc
     6481 aaatggattt tttaatcaat ctcaaaaatc cagacagaag gcaatcaccc agcaaaatta
     6541 ttgattacct gttatcacaa aggcctattg tgaatattag ttcggatttt cccgaacatg
     6601 agcgaaacgt gtttcttgaa tttattcatg gtgattacac ccacagattg aaaaattcca
     6661 atatcgatca atataacatc cagaacgtt
//
LOCUS       DAOSIO010000082         6650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_10469_length_6650_cov_3.164064, whole genome shotgun sequence.
ACCESSION   DAOSIO010000082 DAOSIO010000000
VERSION     DAOSIO010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6650
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10469_length_6650_cov_3.164064"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PK590_04185"
     CDS             complement(<1..354)
                     /locus_tag="PK590_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23828.1"
                     /translation="MEFLKTKSNTKIVSNPRVVVLNNQTAHIQVGSQIPIPSFERNET
                     TGSMEVSGFSFRDVGVVLKVTPHINSAQEIMVDLNPEVSTQGANVVFTPTLAAPSFNV
                     TQASTQVLIQSGQTIA"
     gene            complement(342..1304)
                     /locus_tag="PK590_04190"
     CDS             complement(342..1304)
                     /locus_tag="PK590_04190"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin and TonB N-terminal domain-containing
                     protein"
                     /protein_id="HOG23829.1"
                     /translation="MIFKISKFGFFSVVFVVLMTMVTQTWALDGEQGDLVAGDSVSAV
                     VSSGDADARVSETEASAEPSGDTISLDFKDADINTVLRILSMKSGMNIVSGPEVKGVV
                     TVRLDNVPWDKALNVILRTYDYVYERDGNIIRVTSREKMGQEPVETKTFILNYSKAAE
                     IQSAVQDILTERGRIKVAERTNMVVVTDIPTNLYRIGEVIENLDKVTPQAYVDSKIIN
                     TDSSYVENMGIQWTPSASITGATRPTTFPFSQTGQSSLPGSLQQFFPVVSVDQTAMPP
                     ALPEKPLRILRIPEIFHIRPRKSSMKPSRMEHWIFLSLRQLWNF"
     gene            complement(1301..1684)
                     /locus_tag="PK590_04195"
     CDS             complement(1301..1684)
                     /locus_tag="PK590_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23830.1"
                     /translation="MRNLREPRRFGLMLAGVLFLWVPLADAEVTYDSGNRRDPFVPLE
                     GVDGMYQGSPSGFVLEGIIYDPAGGSFAIINGMPCKAGDMAGEAKVTRIRKNNATLDV
                     NGEMKTLWIRDAEKLEQNDGGEDLS"
     gene            complement(1689..2300)
                     /gene="pilO"
                     /locus_tag="PK590_04200"
     CDS             complement(1689..2300)
                     /gene="pilO"
                     /locus_tag="PK590_04200"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOG23831.1"
                     /translation="MKPLKQWTKQELIDFLKGVDKKTWVVIGASLAGVGILWFFLIGP
                     AWFERPRLRSEIQKMDTQIRQYQMLDQKRLKLEENQKVFFQLLADVQARLFGEEDLAL
                     LLGQISKLAYDARVDIAASKPKAEAVEFPAPYASRYEAVAYDFTMQGGYHQLGKFSAA
                     LESYPKWLRIQKIKITPLKDDALRHLAQFELVAIKDKTAKKAG"
     gene            complement(2297..2467)
                     /locus_tag="PK590_04205"
     CDS             complement(2297..2467)
                     /locus_tag="PK590_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23832.1"
                     /translation="MPVSARTGIEQIEGFLQELKSKYPRMDISLTTAKVKEKDAVGTS
                     FSATIKWSAEVS"
     gene            complement(2458..2871)
                     /locus_tag="PK590_04210"
     CDS             complement(2458..2871)
                     /locus_tag="PK590_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23833.1"
                     /translation="MITINLLPQEFQQTRKQAVARPYFPLAILAGSLFMLLTLFFYAD
                     YLKAKSAYDSVHSEWMKLSPQMQKLKTLEQKVEVEMRGEKEFLEHHVLNTDSMTRILQ
                     LVSENLPKRGWLTELTLERAQTGGEIWFSREWYCR"
     gene            complement(2868..3968)
                     /gene="pilM"
                     /locus_tag="PK590_04215"
     CDS             complement(2868..3968)
                     /gene="pilM"
                     /locus_tag="PK590_04215"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOG23834.1"
                     /translation="MLPDKLLTKFDIKRLATLLKSFFLMQGRSSLHPSRTTVCDLGRS
                     KIILLELARRPDGLFIEKFELIKNPLQDSQPSLLLKPFFDSKKFSRDNIRVTLKGHGV
                     IMRFIRFPKMKREDLKSALQFEVEQYIPFELKDCVMDYAVVDENVKAEDGEKMEILLA
                     VVKRQDVDPTVEIFRNLECNLSVIDVDILSALSALEYFYPEDVAGHVALLDIGTEIST
                     LGIVMDGKPRFIRDISYGTYDVLKRLRLRTSMPEDEIEGIFDRNTEENSEVVTAMTES
                     LEGFIGDLKVSFDYYREQLHAAKPVGKIFISGGGSLHPILLRVLSGGLGIPAEGMEVL
                     SKLRWDDTVDAEQLRKHQAFLPVALGLALRPE"
     gene            complement(4059..4520)
                     /locus_tag="PK590_04220"
     CDS             complement(4059..4520)
                     /locus_tag="PK590_04220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOG23835.1"
                     /translation="MFIKMNQRGFTLVEIMIVVAIIGLLAAIAIPNLLRARLNANEGS
                     ARSNMRTISSAAESYRAAQATPTYPATDADLTGATPTYLDATIFAGTGKTGYLYDLVG
                     GVNMYQAVANPVTANTTGVNSYCVDHTGVVRTQVGEYTAVNNAACGGTPIA"
     gene            complement(4765..6012)
                     /locus_tag="PK590_04225"
     CDS             complement(4765..6012)
                     /locus_tag="PK590_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOG23836.1"
                     /translation="MPFFEYYVKDKTGKDVKGVEESLDSSELVRKLKAQGYMVIRIVE
                     VNKAQPLFRLAKGTSATPDKKQAKGKSGSITIDDMVVFARQLATLVAAGIPLVQSLDI
                     LSKQVDKQQFRVILSKMFTDVQTGKSLSEAMMSYPKVFSGLFIHMVRAGETSGRLQEI
                     LDRVAQYFEKASALQKKVKSALMYPTVVSIMAFGITFAMLAFVIPKFATIFDGLGAKL
                     PAPTQVLIDVSNYLATNWWWILGVLGGCVFLFRQIVRMPAGRLAWDSFQLRMPIFGTI
                     ILKVAISKFSRTLATLIKSGVSILAALEIVTKTSGNARIEKMIVELMAAVKRGESIAG
                     PLEKGEVFPPMVIRMIAIGEETGELEAMLTKIADFYDAQVDAAVAGLTSLIEPLIIAF
                     LGVVIGGIVIALFLPILTLTQAL"
     gene            complement(6016..>6650)
                     /locus_tag="PK590_04230"
     CDS             complement(6016..>6650)
                     /locus_tag="PK590_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOG23837.1"
                     /translation="YQVEGITQTQVIPEIGLTFASGLRALLRQSPDIVLVGEIRDGET
                     ADIAVKAALTGHLVFSTLHTNSAAGAMTRLMDMGIEPFLIASSVVCVAAQRLLRRICP
                     HCKRPATIPDDVLRRCKIPESELKMITVCHGEGCAKCNHTGYLGRLGAAEVLSIDPEI
                     RNLVIQKKSSNVIHETAVHKGMETLFENALGLFKSGATTLEEVLRVTSAG"
BASE COUNT         1717 a         1850 c         1453 g         1630 t
ORIGIN      
        1 ggcgatcgtc tgaccacttt gtatcaaaac ctgcgtcgaa gcctgtgtca cgttaaaaga
       61 cggggccgcg agtgtcggcg taaataccac attggcgccc tgcgtgctaa cctcggggtt
      121 caaatcaacc ataatttcct gggcagagtt gatatgcggt gtcaccttca agacgacgcc
      181 cacatcccgg aaactgaacc ccgacacttc catacttccg gtagtctcat tacgctcaaa
      241 gctcggtatg ggaatctggg aacccacctg gatatgcgcc gtttggttat tgagcacaac
      301 cacccgggga ttggacacaa ttttggtatt cgacttggtc ttcaaaaatt ccaaagttgc
      361 cgcaaactta gaaaaatcca gtgttccata cgcgaaggtt tcattgacga ctttcggggc
      421 cggatatgga aaatttcggg gatccgcaat attcgcaatg gtttttccgg cagcgcgggc
      481 ggcattgcgg tctgatcaac gcttacaacc ggaaaaaatt gctggagact tcccggaagt
      541 gatgattgtc ccgtttgcga aaaagggaaa gttgtcggcc tggtcgcccc cgtaatgctt
      601 gccgacggcg tccattgaat ccccatgttt tccacataac tgctgtcagt attaatgatt
      661 ttcgaatcca cataagcctg aggggtaacc ttatccagat tctcgataac ctccccgatc
      721 cggtagaggt tggtagggat gtcggtcacg accaccatgt tggtgcgctc cgccaccttg
      781 atccgtcccc gctcagtcaa aatatcctgg acggcggact ggatttcggc cgcctttgaa
      841 taattcagga taaaggtttt cgtttctacg ggttcctgtc ccattttttc acgggaggtg
      901 acgcgaataa tgttaccgtc gcgttcgtaa acataatcat aagtacgcaa aataacattg
      961 agcgccttat cccaggggac attgtcaagc cgaaccgtca caaccccttt aacttcggga
     1021 ccagaaacaa tattcatccc gcttttcata ctgagaatgc gaaggaccgt attaatatcc
     1081 gcgtccttga agtcaagact gatggtgtca cccgagggct ctgccgaagc ttccgtttcg
     1141 gacacgcggg catccgcgtc gccggaagat accacagcgg aaactgaatc ccccgccaca
     1201 agatcacctt gttcaccgtc gagggcccat gtttgggtga ccatcgtcat tagcactaca
     1261 aacaccactg agaaaaaacc gaacttgctg attttaaata tcatgacaag tcttctcctc
     1321 cgtcattttg ttccagcttc tccgcgtcac gaatccagag agttttcatt tcaccattca
     1381 catcaagcgt tgcgttgttt ttgcgaatcc gggtcacttt cgcttctccc gccatgtctc
     1441 cagctttgca cggcatgccg ttaataatcg caaaagaccc ccctgccgga tcgtaaataa
     1501 ttccctcgag aacaaatcct gacggacttc cctgatacat cccgtccaca ccttccaagg
     1561 gaacaaaagg atcccgccga ttcccgctgt cgtaggtaac ctccgcatcc gccaaaggaa
     1621 cccacaaaaa cagaaccccg gccagcatca agccaaatct tctcggctca cggagatttc
     1681 gcataagtct atcccgcctt tttcgcagtc ttgtctttaa tagcaaccag ttcaaactgg
     1741 gccagatggc gcaacgcgtc atctttaaga ggcgtaattt ttattttctg aatcctcagc
     1801 catttgggat aagattccag cgcggcagag aatttcccga gctgatgata acctccctgc
     1861 atcgtaaaat cgtaggccac cgcttcataa cggctagcgt aaggcgccgg aaattccacg
     1921 gcttcggctt tgggctttga cgccgcaatg tccaccctgg catcatacgc aagcttcgaa
     1981 atctggccta aaagcaaagc aagatcctct tccccaaaaa gacgcgcctg gacatcggcc
     2041 aggagctgga aaaaaacctt ctgattttct tcgagcttca accgcttctg atcaagcatt
     2101 tgatattgtc tgatctgggt gtccatcttc tgaatttcac tccgcaaccg gggccgctca
     2161 aaccacgcgg gtccaattaa aaaaaaccac aaaataccca cacccgctag gctggcgcct
     2221 ataaccaccc acgttttctt atcgacacct tttaaaaaat ctataagctc ctgttttgtc
     2281 cattgtttca acggcttcat gagacctcgg cgctccattt tatagtggct gaaaacgagg
     2341 ttccgacagc gtccttttcc ttcaccttgg cggtcgtaag cgagatatcc atccggggat
     2401 acttggattt aagttcctgt aaaaaccctt cgatttgctc aatgcccgtt cttgcactta
     2461 ccggcaatac cactccctgg agaaccaaat ctcccccccc gtttgggcac gctcaagggt
     2521 aagttccgtg agccagcccc gcttcggcag gttctcgctc acaagctgaa ggatccgggt
     2581 cattgaatcc gtgtttaaaa catgatgttc caaaaattct ttttcacccc gcatttcaac
     2641 ttccaccttc tgttccaatg tcttcagttt ctgcatttgc gggctgagct tcatccactc
     2701 gctgtggaca ctgtcataag cgcttttcgc tttaagataa tccgcgtaaa aaaacaaagt
     2761 aagtagcata aaaagcgaac ctgccaaaat cgcgagcgga aaataaggtc gtgccaccgc
     2821 ttgtttccga gtttgctgaa attcctgggg gagaagattg atagtaatca ttcgggcctc
     2881 aatgcaagac caagagccac gggtagaaaa gcctggtgtt ttcggagttg ttcagcgtcc
     2941 acggtgtcat cccagcgaag cttggacagc acttccattc cctctgcggg aattccgagc
     3001 cctccgctca gcacacgcaa caaaatagga tgcaaggagc caccaccgct tataaaaatc
     3061 tttccgaccg gcttcgccgc gtgaagctgt tcccggtaat aatcaaagga aaccttcaga
     3121 tcgccaataa aaccttccaa actctcggtc atcgcggtaa caacctccga attttcctcg
     3181 gtattccggt cgaaaatgcc ttcaatctcg tcctccggca tgctggtcct caatcgcaaa
     3241 cgcttcagga catcatacgt cccataggaa atatcccgga taaaccgcgg cttcccgtcc
     3301 atcacgattc caagcgtcga tatttcggtg ccaatatcca gaagcgcgac atgccctgcc
     3361 acgtcctcgg gataaaaata ttcgagcgcg gagagtgccg acaaaatgtc aacgtctatc
     3421 accgaaagat tgcattccag atttctgaag atttcaaccg taggatccac atcctggcgc
     3481 ttcaccacag ccagaaggat ctccatcttt tccccgtcct ccgccttcac attctcgtcg
     3541 acaacggcgt aatccatcac gcaatctttc aattcaaaag ggatgtactg ctccacttca
     3601 aactggagag cgctcttaag atcttcccgt ttcatctttg gaaaacggat aaatcgcatg
     3661 atcacgccat gccctttcaa agtaacacgg atattgtcac gactgaactt tttggaatca
     3721 aaaaatggtt tcagcaacag ggatggctga gaatcctgga gcggattttt tatcaattcg
     3781 aacttttcga tgaaaagccc atcggggcga cgcgcaagct ccagaagaat aattttggac
     3841 cgtcccagat cgcagactgt agtacgcgaa ggatgcaaag acgagcggcc ttgcatcaaa
     3901 aaaaaagact taaggagcgt cgcaagccgt tttatgtcaa atttagtgag tagtttatct
     3961 ggaagcatgg ggcacataat aacataagtg gttaaaaaaa acccgccctg cttttaagag
     4021 cagggcgggt tctacaactt acacctcaga aacacttgtt acgcgatcgg cgttccacca
     4081 caggcagcgt tgttaaccgc agtatactcc ccaacctgtg ttctcaccac tccggtatga
     4141 tcaacgcaat aactgttcac accggttgta ttcgcggtta caggattcgc aaccgcctgg
     4201 tacatgttaa caccaccaac aagatcatac agatatcctg tttttccggt tccggcaaaa
     4261 attgtcgcgt ccagatacgt cggtgttgcg cccgtcagat ccgcatcagt cgccggataa
     4321 gtcggtgttg cctgggccgc acgatagctt tccgccgcgg aggaaatggt cctcatgttc
     4381 gatctcgccg aaccttcatt ggcattcaac cgagcacgca gcaagttagg aatagcaatc
     4441 gctgccaaaa gcccaataat cgcgactacg atcatgattt ccaccaatgt gaaaccacgt
     4501 tgattcatct ttataaacat atgctacctc ctgttattta ccatggcctg ctgttagtat
     4561 aattcattaa aacctaatcg gcaatgcctt gtcaatcctt tagaagaaaa taactctctt
     4621 tgggcctctc ttaataaagg cttgaagtaa gtatctttca cgaatacccc acgggtctag
     4681 cacaacccca ttcattgtcc caaatattaa atcaaacttt cacgggtcga atacggtctg
     4741 aaacgtcaga tgcgcagtac gaacctaaag agcctgggtc agcgtaagaa tcggcaggaa
     4801 aagagcaatc acgattcctc cgataacaac cccaagaaac gcgataatga gcggttcgat
     4861 caatgaggtc aatccagcga ccgcggcatc cacctgggca tcgtaaaagt cagcgatttt
     4921 ggtaagcatg gcctcaagct ctccggtctc ttctccgatc gcgatcatgc gaatcaccat
     4981 cggggggaaa acctctccct tttcaagagg tccggctatg ctttctccgc gttttacggc
     5041 cgccatcaat tcaacgatca ttttttcgat acgggcgttt ccggatgttt tcgttacgat
     5101 ctcgagcgcc gccagaatgg acacaccact tttaatcagg gtcgcaagcg ttcgggaaaa
     5161 tttactaatc gccaccttca ggataatcgt cccgaagata ggcatccgca actgaaaaga
     5221 gtcccaggca agtcgccccg ccggcatccg cacaatttga cggaagagaa aaacacagcc
     5281 tcccaacact cccagtatcc accaccagtt tgtcgccaaa taattactca catcaatcaa
     5341 gacctgggtt ggcgccggaa gcttggcgcc cagaccatca aaaatggtcg caaacttggg
     5401 aatcacgaaa gcgagcatcg caaaagtaat cccgaacgcc atgatggaaa caaccgtcgg
     5461 atacatcagc gccgatttaa ctttcttttg aagcgcgctt gccttttcga aatactgagc
     5521 cacccggtca aggatctctt gtaaccgccc gctggtttca cccgctcgaa ccatgtgaat
     5581 aaacaacccg gaaaaaactt tcgggtagga catcatggcc tctgacagac ttttccccgt
     5641 ctggacgtcc gtgaacattt ttgagagaat aacgcgaaac tgttgcttgt caacttgctt
     5701 ggaaagaata tcgagcgatt gaaccagcgg tatcccagcc gccaccagag tcgccaattg
     5761 ccgggcaaaa acaaccatgt catcgattgt gattgagccg gatttccctt tcgcctgttt
     5821 tttatccggt gttgccgacg tccccttagc cagtcgaaaa agaggttgcg ccttgtttac
     5881 ctcgacaatc cggatcacca tgtaaccctg cgctttgagt ttccgtacga gttccgatga
     5941 atcgagcgat tcttcgacgc ctttaacgtc cttgcccgtt ttatctttta cgtagtattc
     6001 aaaaaatggc atgatttatc ccgcgctggt gacgcgcaag acctcctcaa gggtcgtggc
     6061 gccggacttg aaaagtccaa gagcgttttc aaaaagtgtt tccatcccct tatgaacggc
     6121 cgtttcatga ataacattac tcgacttttt ctgaatcacc aggtttcgga tttccggatc
     6181 aatggataat acttcagccg cgccaaggcg tccaagatat cccgtatgat tacacttcgc
     6241 gcacccttct ccatgacaaa cagtgatcat tttaagttca gattccggaa tcttacagcg
     6301 gcgcaaaaca tcgtccggaa tggtggccgg ccttttacag tgcgggcata ttctccgcaa
     6361 taatctctgc gcggcaacac agacaacaga agatgcgatc agaaacggct caattcccat
     6421 gtccatgagg cgcgtcatcg cgccagccgc gctgttggtg tgtagcgtgc tgaacacaag
     6481 atgaccggtc agggcggcct tgaccgcgat atcagccgtt tccccgtcgc gaatctcccc
     6541 gacaagcaca atgtcgggac tctgtcgcaa aagtgcccgc aacccgctgg caaatgtcag
     6601 gccaatctcg ggaataacct gggtttgcgt aatcccctcc acctgatatt
//
LOCUS       DAOSIO010000083         6608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_10558_length_6608_cov_4.924615, whole genome shotgun sequence.
ACCESSION   DAOSIO010000083 DAOSIO010000000
VERSION     DAOSIO010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10558_length_6608_cov_4.924615"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PK590_04235"
     CDS             complement(<1..478)
                     /locus_tag="PK590_04235"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG23838.1"
                     /translation="MPLTKLPYFDIKRQHEPMQGEVMAALEKVYASGAYILGPALKDF
                     ECQYAGYVGTPYAAGVSSGTDALILALKAAGVKAGDEVIVPSFTFTATALAPLHLGAV
                     PVFADVDPISFTLDPQSLQKALSRKTRAVIPVHLFGQCADMDAIGRIAKSHRIKVIE"
     gene            complement(480..1247)
                     /locus_tag="PK590_04240"
     CDS             complement(480..1247)
                     /locus_tag="PK590_04240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG23839.1"
                     /translation="MEKAPVSIAIIAKNEEKRLAECLASAAFAAEIIVLDDMSTDRTV
                     EIAEQAGAKVFQRAMDIEGRHRNYAYSLATQPWVFSLDADERITPELAREIAEVVNRP
                     TDPNTCYCMPVKTFIGADWIKGGGYYPAPKTRLFKKGQFRYEEARVHLRMIYEGTCGS
                     LKGDLLHYSSPSWETWLAKFNRETGLEAEKWIKDGRKVGPWRVLRKAASRFLKYYFQK
                     DGIKNGYTGFFMSFMHSTYQIITYAKYRELKAKNAAK"
     gene            complement(1327..2721)
                     /locus_tag="PK590_04245"
     CDS             complement(1327..2721)
                     /locus_tag="PK590_04245"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HOG23840.1"
                     /translation="MRSAYSRKRILGVVLVDVAMIILSFLVAYFVRFSWILPTDRGVP
                     GVLAYSHVLFVIVPVFLWFFREARLYEPARHVRRIEDIFLVIKSVTYAVIVLTAATFF
                     YRDYTYSRLGLLILWFFACALISAGRYGVIQWEYVRRRQKKDITEVLIIGAGQNAHRL
                     IRWAKNNPHYGLEVVGVLARDPANIGKHLEDVPIVGLASEWEAWIDKLQPGRVVLLDP
                     DFSRETVTELVVTCEDRMIEFKIGADTHGLIARNVGVEYISNIPLLGFKPLPLDDPWN
                     RFVKRSFDLVLATVAGIVFLPFGAGIAALIKMTDRGPVFYRQERIGRDGKTFGLLKFR
                     TMKVDAEKETGPVWAKKNDERRTRFGEFLRRTNLDEVPQLWNIIRGDMSLVGPRPERP
                     HFVNQFREVVPRYMARHKIKSGLTGWAQVHGLRGDTSIHNRVKYDLYYTENWSLLLDI
                     EIMVMTLFAFKNAY"
     gene            complement(2718..3812)
                     /locus_tag="PK590_04250"
     CDS             complement(2718..3812)
                     /locus_tag="PK590_04250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG23841.1"
                     /translation="MMKVALVHDWTIHMRGGEKVLDALAEIFPEATLYTLFSDPKRLS
                     PRLRRLRIRNSFLQYLPGIRCYYRWLLPILPLAIRSLKLDSADVVISSSHCVAKGICK
                     PAGAIHICYCHTPARYLWGFEGVYFDRYPGLIRRLIGLLLQWLRAWDLSVNKDVDLFI
                     ANSDCVKERIRHFYKRDALVVHPPVETRFYTPSAVREDYYLAVSHFVPYKRLDLVIEV
                     FNRLDRKLLIAGSGPMERAYRAMKKSERIFFLGAVTDEELRALYAGARALIFPAEEDF
                     GIVPVEAQSCGTPVIAFGRGGSLETVKTGVFFDEQTADSLEDAILRFEVLEPDPALTR
                     ERVESFGRNEFLDKIRQIVHSRHLHTRKSL"
     gene            complement(3809..4942)
                     /locus_tag="PK590_04255"
     CDS             complement(3809..4942)
                     /locus_tag="PK590_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOG23842.1"
                     /translation="MIRPMIARAGLDIRMLRHSGIGTYLRGLLEGFHQAGISPEERPV
                     LFGDPDSASAFSEKKIRAFAAPIYSLREQARYPSRLRQCELWHAPHYNVPLFKGKTKL
                     VVTLHDIIHWIFRKQFLGPVQTAYAGFMLKQAVRTSDHIIAVSEHTKKDLMETFGAPA
                     EKITVIYEGVGRAYRPMEAEELSTRFRTVSEKYRIPEDFFLYVGLMKPHKNVLWLVEI
                     FKKLKAAKRLRSALVLVGRKDRRYPKGYEALRDLETGPGLIYIPEIEFPELLTLYNRA
                     TALVHPSLYEGFGLTLLEAMKCGTPVIASRAASLGEVAGDAAVLVDPRSADEMAEALI
                     KVETNAGLRADLTARGFERVKIFDWAETARQTVAVYRKTLGGQ"
     gene            complement(4939..6102)
                     /locus_tag="PK590_04260"
     CDS             complement(4939..6102)
                     /locus_tag="PK590_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOG23843.1"
                     /translation="MKSPYHYKLGWKRFLAGVFDVFGGAVFFLVRKFVKAPEVKDIKK
                     ILVIRADHVGDVVMTRPAIRSLHKKFPHAEIDLLVDEKIAPLFEDSSKFHQVLKIHHS
                     WFSPKKDFGAKWREFWRLKRFLTKIGYDVAFDFRGDVRHILLMALCHIPYRYGFGVTG
                     GGFLLTENTPYDDSIHQVLLNLSLLKSFQTPQDNKLLPFEYTRERAATFWKNIGEISL
                     ATDLPRVVIHMGAGTAAKRWGTDDYRALIHQLDSGNRAQMILIGTESERELLSDLKLN
                     TAHLIDLRGRTRIEDLPILFDVCDLYVGADSGPAHIAAAHGLEIILLASGTNDIRFWY
                     PWTEHLHILQHEVPCAPCGLRVCPVEGHPCMSEISVDQVYDAVEGVLKKFKNR"
BASE COUNT         1578 a         1983 c         1633 g         1414 t
ORIGIN      
        1 cttcgatcac tttgatacga tgggatttcg cgatccgccc aatcgcgtcc atgtccgcgc
       61 attgtccaaa aaggtggacc gggatcacgg cccgagtctt ccgcgaaaga gccttttgaa
      121 gggattgagg atctagcgtg aacgaaatcg gatccacatc cgcgaacacc ggaacggccc
      181 ccaggtgaag gggggcgagc gcggtcgccg taaatgtaaa cgacggaaca atcacttcat
      241 cgcccgcctt gacacccgcc gctttcaggg cgaggatcag cgcgtcggtt cccgacgaaa
      301 ctcccgcggc atatggggtc ccgacatacc ccgcgtattg acactcaaaa tccttgagcg
      361 cggggccgag gatataggcg ccgctcgcgt aaaccttttc gagcgcggcc atgacctcgc
      421 cttgcatcgg ctcatgctga cgcttgatgt cgaagtacgg cagtttcgtg agcggcaaat
      481 tactttgccg cgtttttggc tttaagttcg cggtatttcg cataggtgat gatctggtaa
      541 gtggagtgca tgaacgacat gaagaaaccg gtgtagccat ttttgatccc gtccttttgg
      601 aaataatatt tgagaaaacg actggcggct ttccggagaa cgcgccaggg cccgaccttt
      661 cgtccgtcct tgatccattt ttcggcctcg aggccggtct cgcgattaaa cttggcgagc
      721 cacgtctccc agctcgggct ggaatagtga agcaaatccc ccttgagaga accgcaggtg
      781 ccttcataga tcatgcgcag gtgaacacgg gcctcctcgt accggaactg tccttttttg
      841 aacagccggg ttttcggggc tggatagtaa ccgccgccct tgatccagtc cgctccgata
      901 aaagttttta cgggcatgca atagcaggtg ttcgggtcgg tcgggcgatt tacgacttcg
      961 gcgatctcgc gagcgagctc cggagtgatc cgctcgtcgg cgtcgagtga aaagacccac
     1021 ggctgggtcg cgagactgta ggcgtaattg cgatgacgcc cctcgatatc catcgcccgc
     1081 tgaaacactt tggcgccggc ctgttccgcg atttcgaccg tgcggtccgt gctcatgtca
     1141 tcgagcacga tgatctcggc cgcgaacgcg gcgctcgcga ggcattcggc aagacgtttt
     1201 tcctcgtttt tggcaataat ggcaatcgaa acaggcgctt tttccataaa ttagtttcct
     1261 gtgcgggaga gaagccgtga aaaaccgatc cgtgttcgct ccccgttttt tattttccgg
     1321 tttttgttaa tacgcgtttt taaaagcaaa gagggtcatc accatgattt caatgtcgag
     1381 cagtaacgac cagttttccg tgtaataaag gtcatatttg acgcggttgt gaatggacgt
     1441 gtcgccccga agaccgtgca cctgcgccca gcccgtgagc ccggatttaa ttttgtgacg
     1501 ggccatgtac cgcggaacaa cttcccggaa ctgattgacg aagtgcgggc gctctggacg
     1561 gggtccgacc agactcatgt caccccggat aatgttccaa agctgcggga cctcgtcgag
     1621 attggtgcgc cggagaaatt ccccgaagcg ggttctgcgc tcgtcatttt ttttcgccca
     1681 caccggcccc gtctcttttt cggcatcgac tttcatcgtg cgaaacttca aaaggccgaa
     1741 cgtttttccg tcacgtccga tcctctcctg ccggtaaaaa accggccccc gatccgtcat
     1801 cttgatcagc gcggcgattc ccgccccgaa cggaagaaac acaatcccgg cgacggtcgc
     1861 gaggacaaga tcaaaggaac gtttcacaaa ccggttccac ggatcatcca gcggaagcgg
     1921 cttgaacccg aggagcggaa tattgctgat gtactccacg cccacatttc gcgcgatcaa
     1981 tccgtgggtg tccgcgccaa ttttaaattc gatcatccgg tcctcgcacg tgaccaccag
     2041 ttccgtgacc gtctcacggg aaaaatcggg atcgagaaga accacccggc ccggctgtaa
     2101 cttgtcgatc caggcctccc actccgatgc caatcccacg atcggcacat cctccaagtg
     2161 cttgccgata ttcgccgggt cccgcgccag aacccccaca acctcaagtc cgtaatgagg
     2221 gttattcttc gcccatcgga taagcctgtg agcgttttgc cccgctccga taatgagaac
     2281 ctccgtgatg tcttttttct gccgcctgcg cacgtactcc cactggatca ctccataacg
     2341 tccggcgcta atcagcgcac acgcgaaaaa ccacagaatc aaaagcccca gccgcgagta
     2401 cgtatagtca cgataaaaaa acgtggcggc cgtcagcacg atgaccgcgt acgtcacgga
     2461 tttaatcacg agaaagatat cttcaatccg ccggacatgc cgcgccggct cgtacagccg
     2521 ggcctccctg aaaaaccaga gaaaaaccgg aacgatcaca aagagcacgt gagaatacgc
     2581 cagaaccccg ggaaccccac gatccgtcgg caggatccac gaaaaacgga caaagtaggc
     2641 cacgagaaaa ctcaggatga tcatggcgac atcgaccagg acgaccccca gaattctctt
     2701 gcgtgaatac gcgcttctca taagctcttc cgtgtgtgaa ggtgacggga atgaacgatc
     2761 tgccttattt tatccaaaaa ctcgtttctt ccaaacgatt ctacgcgttc ccgcgtcaag
     2821 gccggatccg gttcgagaac ctcaaatcgc aaaatcgcgt cttccaggga atcggccgtc
     2881 tgctcatcaa aaaaaactcc ggtcttgacc gtctcgagcg aaccgcctcg cccgaacgcg
     2941 atgacgggag tcccgcagga ttgcgcctcc accggcacaa ttccgaaatc ctcttcagcc
     3001 ggaaaaatga gcgcccgcgc cccggcataa agcgcccgaa gctcctcatc cgtcacggcg
     3061 cccagaaaaa aaatccgctc gcttttcttc atcgcccggt acgcccgctc catcggcccc
     3121 gaaccggcaa tcaaaagttt tcgatccaaa cggttaaaca cctcgatgac aagatccagc
     3181 cgcttatacg gcacaaaatg cgagaccgcc agatagtaat cctcccggac cgccgaaggg
     3241 gtgtaaaacc gggtttcaac cggaggatga accaccagag cgtcccgctt gtaaaaatgg
     3301 cgaatccgct ccttcacgca atcggagttc gcgataaaca aatccacgtc cttattcacg
     3361 gaaaggtccc acgcgcggag ccattgaaga agcaacccga tcaaccggcg tatgagcccg
     3421 ggataacggt caaaataaac accttcaaat ccccagagat accgcgcggg cgtatgacaa
     3481 taacaaatgt gaatggcccc cgccggcttg caaatcccct ttgccacaca atgagaactc
     3541 gaaataacga catccgccga atcaagtttc agggagcgga tcgcgagggg caggatcggg
     3601 aggagccacc ggtaatagca ccgaataccc ggaagatact gaaggaatga attccggatc
     3661 ctgagccgcc gcaacctggg actcagccgc ttggggtccg aaaaaagcgt gtaaagagtc
     3721 gcctcgggaa aaatctcggc gagcgcgtcc agcacctttt caccaccccg catgtggatt
     3781 gtccagtcgt ggaccagggc aactttcatc attggccgcc cagtgtctta cggtaaaccg
     3841 cgacggtctg acgcgccgtc tcagcccagt caaaaatttt gacgcgctca aatccccgag
     3901 ccgtcaaatc agcccgcagt ccggcatttg tttcaacctt gatcaaagcc tccgccattt
     3961 catccgcgga tcgcgggtcc acaaggaccg ccgcgtcccc cgccacctcg cccaacgacg
     4021 cggcgcggct ggcaatcacg ggagttccac acttcatggc ctcaagcagt gtcaatccaa
     4081 acccttcata aagcgacgga tgcaccaggg cggtcgcccg attataaagc gtcagcagtt
     4141 ccggaaactc aatctccgga atgtagatca ggcccggccc cgtctcaagg tcccggagcg
     4201 cctcgtatcc tttcgggtac cgccggtcct ttcgtcccac cagcaccagc gcggatctta
     4261 accgcttggc cgctttcagt tttttgaaaa tttcaacaag ccaaagcaca ttcttgtggg
     4321 gcttcataag gcctacgtat aaaaaaaagt cttccgggat ccggtacttt tcactcaccg
     4381 ttctgaaccg cgtcgaaagt tcttccgcct ccatggggcg atacgccctt ccgacccctt
     4441 cgtaaatcac cgtgattttt tcggcgggag cgccaaacgt ctccatcaga tcttttttgg
     4501 tatgttccga caccgcgatg atgtgatccg aagttcgaac tgcctgcttc agcatgaaac
     4561 cggcgtaagc cgtctggacg ggaccaagga actgctttct gaaaatccag tgaatgatgt
     4621 cgtggagggt cacaaccagt tttgtcttcc ccttgaaaag cggcacattg taatgcggcg
     4681 cgtgccacaa ttcacactga cgcaagcgag acgggtatcg cgcctgttcc cgtaatgaat
     4741 aaatcggagc ggcaaaggcg cggatctttt tttctgaaaa agccgaagcg ctgtccggat
     4801 caccgaacag aacgggccgc tcctccgggg aaatgccggc ctgatgaaac ccttcaagga
     4861 gtccgcgcaa atacgtcccg atccccgagt ggcgcaacat cctgatatca agtcccgcgc
     4921 gggcgatcat aggacggatc aacgattctt aaactttttc aagacaccct caaccgcgtc
     4981 atacacctga tccacactaa tctcactcat gcagggatga ccttccaccg gacacacccg
     5041 cagaccgcaa ggggcgcacg ggacttcgtg ctggaggatg tggagatgct cggtccaggg
     5101 gtaccagaat cgtatgtcat tcgttccgct cgcgaggaga atgatttcga gtccgtgggc
     5161 ggccgcgatg tgagctggcc ctgagtcggc accgacatac aggtcgcaga catcgaacag
     5221 gatcgggaga tcctcgattc gggtcctccc acgcagatca atcaggtgag ccgtattcag
     5281 tttcaggtcc gacaaaagct cgcgctcaga ttcggttcca atcaaaatca tctgggcccg
     5341 gttccccgaa tccagttgat gaatgagcgc gcgataatca tccgttcccc accgttttgc
     5401 cgcggtcccc gcgcccatgt ggattacaac tctcggcagg tcagtcgcga gggaaatttc
     5461 ccctatattt ttccagaagg tggccgcgcg ttctctcgta tattcaaagg gaaggagctt
     5521 gttatcctgc ggcgtctgga aagacttgag caaacttaaa ttcagtagca cctgatggat
     5581 tgaatcgtca taaggcgtat tttccgtcaa caaaaatccc ccgcctgtca ctccaaaccc
     5641 gtagcgatac ggaatatggc aaagggccat caaaaggata tggcggacgt cacctcggaa
     5701 atcgaacgcc acatcatagc cgatcttggt cagaaagcgt ttgagccgcc aaaactcccg
     5761 ccacttcgcc ccaaaatcct tcttcggcga aaaccagctg tggtgaattt tgagcacctg
     5821 atgaaacttg cttgaatcct caaagagcgg cgcgatcttc tcgtccacca acaggtcaat
     5881 ttcggcgtgc ggaaattttt tatgcaggct ccggatcgcg ggccgggtca tgaccacatc
     5941 cccgacatga tcggcgcgaa tcacgaggat tttcttgata tctttgactt ccggcgcctt
     6001 cacgaatttt cggaccagaa aaaaaacagc tccgccgaaa acgtcaaaca caccggctaa
     6061 aaaccgcttc cacccaagct tgtagtgata aggactcttc ataatagctt caatattcct
     6121 acatccttct cttttttgca aacaaagaaa catcctcccg ttctcgcttc cttccttgca
     6181 aaaaaagggc aacccagctc acctcccctc tttgtaaaag aaggccacca aactcccccc
     6241 ttttttttaa agaggccaac ccaactcgct cccctctttg taaaagaggg gctgggggag
     6301 atttcttcca ctcaacccca aatccccctc actccccctt tttcaaaggg ggaaacatgt
     6361 tggctcccct ctttgtaaaa gaggggctgg gggagatttc ttccactcaa cctcaaatcc
     6421 ccctcactcc cccttttcca aagggggaaa catatctact cccctctttc ttaaagaggg
     6481 gctgggggag atttcttcca ctcaacccca aatccccctc actccccctt tttcaaaggg
     6541 ggaaacatgt tggctcccct ctttgtaaaa gaggggctgg gggagatttc ttccactcaa
     6601 cctcaaat
//
LOCUS       DAOSIO010000084         6570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_10652_length_6570_cov_4.879355, whole genome shotgun sequence.
ACCESSION   DAOSIO010000084 DAOSIO010000000
VERSION     DAOSIO010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10652_length_6570_cov_4.879355"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PK590_04265"
     CDS             <1..949
                     /locus_tag="PK590_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG23844.1"
                     /translation="AIAVGFGIKYLPPDRFVAFVGSLFIFYEPLKKISKVNSTIQQSI
                     AAGQRIFEVLDSRSTIQEMPGALDFKEPVREVVYENVSFRYEEGDAAALHGINILAKR
                     NEVIAIVGASGSGKTTLMHLLLRFYDPSEGAIKINGKDIRELSLKSLRDMIGIVSQET
                     VLFNCSVRDNIAYGRPNAGLEDVKRAAAIAYADHFIENLPNGYDTQLGERGLKLSGGE
                     RQRLAIARALIKDPPILILDEATSHLDTQSEKEVQGALENLMKERTVFVIAHRLSTVQ
                     KADRILVMEKGKIVQQGTNEFLLRLGGAYKKLYDLQFNL"
     gene            1005..1628
                     /locus_tag="PK590_04270"
     CDS             1005..1628
                     /locus_tag="PK590_04270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23845.1"
                     /translation="MKKISRKKKTKSLKSVPLEKKDRFSDLLALMTQLGARFESLEKK
                     VEMVLSKGQAFLSRGVVAPENHHPVSSGVRPDKPSDEPPSVGGPVPVKQANASGRRKN
                     LFQAVCADCRKTCEVPFKPTGERPVYCNECFRARKSFGRSDKSTAGNGAPANCPQEVQ
                     RRVTVIKKGAGKLTISDIVPVQEKAQKSVARHRRPAKTHTKTRGKNV"
     gene            1621..1782
                     /locus_tag="PK590_04275"
     CDS             1621..1782
                     /locus_tag="PK590_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23846.1"
                     /translation="MFDLNKLGDMAKIAGEAKSMQEKQERMAREQLETLKKISGQLET
                     VISLLKEKK"
     gene            complement(1864..2289)
                     /locus_tag="PK590_04280"
     CDS             complement(1864..2289)
                     /locus_tag="PK590_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23847.1"
                     /translation="MKKISLIGLILMTAFFNAAMARSDQYDEDARAQEKTQKQLAQEA
                     RAPEKRTPLKTIGSGIKEITYDNVRDVTKDTTQGAVSKTPVIGTLDGTQQAGEKVVDN
                     TIRGVKKVASFGLAKDDSYTIEEAEKGSGDAAKIKLFQF"
     gene            2606..3190
                     /locus_tag="PK590_04285"
     CDS             2606..3190
                     /locus_tag="PK590_04285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003201503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOG23848.1"
                     /translation="MNCLIIYSHPNPASFNHAIKEALVKSLKEKGKNVRTRDLYAMRF
                     DPVLKADDFETLSRDKTPRDIEIEQEHIRWADVLVFIYPIWWAGMPAITRGYVDRVLI
                     KGFAYEETPQGSKGLLQAKKVYIINTMGAPLSVYEEMGIFKAMDMIYDQQLFGFCGFK
                     VLGHKYFGSVQSVSDEERKQMLAEIDGIVAGFEG"
     gene            3196..4974
                     /locus_tag="PK590_04290"
     CDS             3196..4974
                     /locus_tag="PK590_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HOG23849.1"
                     /translation="MTPSTFVGTYRIRDSKVIIRIKNQICDSAEELLGSSLCFEIIKR
                     AVESLRKRESILLKVFGNRELNEETLNLLVRIFQFLEKMEAEHVVRVVPGAEDFFKDR
                     NLLNDFVEYLYNFWRSYERFIVCDSEGDQFDKRPYRTFNRTIENLTELVRHTYREIQE
                     NITGTHPRIYRQIAAGAEMATIATPKPVPYPEGSYEKLLPIGMIRQILFYPPLVLNPP
                     NNKRTGKFERIQENPMDWVHLNREEWLCYPVKAGDFLAHVYFSEKFFELGLSMANLFE
                     LASDEDLKRKPDAVYLYGVAEDQFKARDVFPTVFYEDEKNGMLVGAVPAKDEFGYFGY
                     LKKMILTLHNIKKIRSGKMPFHGAFMKVFLKGKKPATLLLIGDSGAGKSETLEAMRGF
                     GADLIQDVIVIADDMGSLEMKESGDVVGFGTETGALSRLDDLKPGFALGQIDRAIIMN
                     PHEVNARIALPITSFANIIQEHAVDFVLYANNYEDIDEDHPVIERFQTVEEAIKVFRD
                     GAVMSKGTTKTVGLVHTYFSNVFGPVQCKAEYDAIAKKFFEAFFKKNIFVGQIRTRLG
                     VHGWERKGPEESAVALLELIRNDQRY"
     gene            5033..>6570
                     /locus_tag="PK590_04295"
     CDS             5033..>6570
                     /locus_tag="PK590_04295"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HOG23850.1"
                     /translation="MTQDVYICLHGHFYQPPRENPWIEEIEQQDSATPFHDWNERIHY
                     ECYLPNSRARALDAKGKIVEIVNNFERSSFNFGPTLLSWLEAKHPDTYKSIIRADQVS
                     RELHRGHGNAIAQVFNHMIMPLANLRDKRTQVRWGLEDFRHRFGREPESIWLPETACN
                     EDTLEVLVEEKIKFIILSPYQARAVRSMEGGEWKDVTGGHIDPRNPYRCFLKNNPSKY
                     LDIFFYDGPISRAIGFDNLLFDAKLFMDRLQGAVVKDGGPQLIHIATDGETYGHHKPF
                     GDRVLGFLTYIEVPKRGLKLTNYGEYLENYPPKNEVRLIEGENGEGTAWSCAHGVSRW
                     KDHCGCRGGGPANWTQHWRKPLRWALDWLRDELARVYEKEGSKYLKDVWAARDDYIRI
                     ILDRNEKTIRSFFYKHAQRELGKTEVTACLKLLEMQRYLMLMYTSCGWFFSEISGVET
                     VQILQYAARAVQLGEELSGAHYESEFLEHLALAKSNIEMFKDGRGVYEKLVRPAMATL
                     QHIV"
BASE COUNT         1654 a         1481 c         1818 g         1617 t
ORIGIN      
        1 ggcgattgcg gtaggatttg gaattaaata tctgccaccc gaccggtttg tggcttttgt
       61 cggctctctg tttatttttt acgagcccct gaaaaaaatc agcaaagtca acagcacgat
      121 tcagcaatcc atcgccgcgg gacagcgcat ttttgaggtt ctggattcga gatcaacgat
      181 ccaggaaatg ccgggagccc ttgattttaa ggagcccgtt cgtgaagtcg tttacgagaa
      241 tgtcagtttc agatatgagg aaggtgatgc cgccgctctt catggaatca atattctggc
      301 aaaacgtaat gaggttatcg ccattgtggg cgcgagtgga tcgggaaaga cgacgttgat
      361 gcatttgctt ctgcgttttt atgatccttc cgaaggggcg atcaaaatta acggaaaaga
      421 catccgggaa ctaagcctta aatcactgcg ggacatgatc gggatcgtgt cgcaggagac
      481 ggtgcttttc aactgctcgg ttcgggacaa catcgcttat ggacggccga atgccgggct
      541 tgaagacgtt aagcgggcgg cggccatcgc ttacgccgat cattttatcg aaaatcttcc
      601 caacggttat gacacccagc ttggcgagcg ggggctcaag ctctccggag gagaacgcca
      661 gaggcttgcg attgcccgtg ccctgatcaa agatcctccg atcctgattc ttgatgaagc
      721 gacctcccat cttgatacac aaagcgaaaa agaggtgcaa ggagcccttg aaaacctcat
      781 gaaagagcga acggtatttg tgatcgccca ccgtctgtcc accgtccaga aggccgatcg
      841 aattcttgtg atggaaaaag gcaagattgt ccagcaggga accaacgaat ttctgcttcg
      901 tcttggggga gcgtacaaga aactttatga tttgcaattc aacctttgat cccggacgtt
      961 gtgatcctcc gcaatttgcc gtcaagtttt gtctaaagtg atttatgaaa aaaatctcca
     1021 gaaaaaagaa aactaaaagt cttaaaagcg ttcctcttga gaaaaaggat cgtttttctg
     1081 atctcctcgc actcatgacc caactgggcg ctcgctttga atccctggaa aagaaagtag
     1141 aaatggtatt gtcaaaaggc caggcgttct tgtcccgggg cgtggtcgca ccggaaaatc
     1201 atcaccccgt atcttccggt gttcgtcctg acaagccatc ggatgagccg ccttcggttg
     1261 gcgggcctgt tccggtgaag caggcgaacg cctcaggtag gcggaaaaat ctttttcagg
     1321 cggtctgcgc ggattgtcga aaaacttgtg aagtcccttt caagcctacg ggggaaagac
     1381 ccgtgtattg taacgaatgt ttcagagctc gtaagtcttt cgggagatct gacaaatcaa
     1441 cggcgggaaa cggagccccg gcgaattgtc cacaagaggt tcagcgtcgg gtaacagtga
     1501 taaaaaaagg ggccgggaag ctgacgatct cggatattgt tcccgtccag gagaaggcgc
     1561 agaaatccgt tgccagacac cgacgcccgg caaaaactca taccaagaca agggggaaaa
     1621 atgtttgatt tgaacaagct gggagatatg gccaagattg ccggagaggc aaagagcatg
     1681 caggagaagc aggagagaat ggcgagagaa cagcttgaga cgctcaaaaa aatatccgga
     1741 caattggaaa cagttatctc tttactgaaa gagaaaaaat aagtttcttc aaacgggtaa
     1801 caagacaaaa aaataaccga cgctcaagaa gggcgccggt tatttttttt aaaacagatt
     1861 gaattaaaac tgaaacagtt tgattttagc ggcgtctccg gaaccttttt cggcctcctc
     1921 aatggtgtaa ctgtcgtctt tggccagccc aaaagatgcg acttttttca cgccgcggat
     1981 tgtgttgtcg acaacttttt cgccggcctg ctgggttccg tccagcgttc cgatgacggg
     2041 cgtttttgag acggccccct gggtggtgtc tttagtgaca tctctgacat tgtcgtacgt
     2101 aatttccttg attcccgaac cgatggtctt gagaggagtc cgtttctccg gcgcgcgggc
     2161 ttcttgagcg agctgttttt gagttttttc ttgggcgcgg gcgtcctcat catactggtc
     2221 gcttctggcc atggcggcat tgaagaacgc ggtcattaga attaggccta tcagagaaat
     2281 ttttttcatg gaaataatcc tcgtttcgct tttaatactt cgtctgatgt tgataactgg
     2341 atgatccccg agtttactgg gggcgtagct gaaatcccat acgcgtgtgc ggggggaagt
     2401 atacatgatt cttgcgcaaa ggaaaagaac gcactcagga tccgcgcgcc atgtaaactg
     2461 tttcccattt cagagagaat tcggctataa ttgccgcatc ctcaaggcga caaggttggt
     2521 ccgttcgaga tcccggtaaa cgatgctccg tgaaggagtg ttttttaaga tgtccccggc
     2581 aggcacatga aaggagattt gggaaatgaa ctgtttgatt atttatagcc atccgaatcc
     2641 ggcaagcttt aatcatgcca tcaaggaagc tctcgttaaa agccttaaag aaaaggggaa
     2701 gaacgtccgt acccgggatc tttacgctat gcgttttgat ccggtactta aggccgatga
     2761 tttcgaaact ctttcgcggg acaagacccc ccgtgacatt gaaatcgagc aggagcacat
     2821 tcggtgggct gacgtgcttg tttttatcta tccgatctgg tgggcgggaa tgccggcgat
     2881 tacgcgcggg tatgtggacc gtgtcctcat caaaggattt gcctatgagg aaacacccca
     2941 gggttccaaa ggtcttttgc aggcgaagaa agtttatatc atcaacacga tgggagcgcc
     3001 tctttctgtt tacgaggaga tggggatttt taaagccatg gatatgattt atgaccagca
     3061 acttttcggg ttctgcggtt ttaaagttct cgggcataag tactttggtt ccgtccagtc
     3121 ggtttctgat gaggaacgaa agcagatgct cgcggaaatt gatgggattg tcgcggggtt
     3181 tgagggataa agggcatgac gccatccacg ttcgtaggga cttacaggat cagggacagc
     3241 aaggttatca tccgtatcaa aaatcagatt tgcgactctg ctgaagaact gctggggagc
     3301 agtctttgct tcgagataat caagcgggcg gtcgagtctc ttcgcaaacg cgaatcgata
     3361 ctcctgaagg ttttcgggaa ccgggaactc aacgaagaaa cgcttaattt gcttgtcaga
     3421 atattccagt ttcttgagaa gatggaagcg gagcatgtcg tccgtgtcgt tcccggcgcg
     3481 gaggattttt tcaaagatcg caatctgcta aacgattttg tcgaatatct ttataatttt
     3541 tggcgcagtt atgagcggtt tatcgtttgc gattcggagg gagaccagtt tgacaaacgt
     3601 ccctaccgga cttttaaccg aacgatagaa aacttgacgg aacttgtccg tcacacctac
     3661 cgtgagattc aggaaaatat tacgggcacc catccccgca tttatcggca gatcgccgcc
     3721 ggggccgaga tggcgactat cgcgactccc aaacctgttc cttaccccga aggttcttac
     3781 gaaaaacttt tgccgatcgg aatgatccgt cagatccttt tttatccgcc gcttgttctg
     3841 aatccgccaa acaacaaacg gacgggaaag tttgaaagga tccaggaaaa tcccatggac
     3901 tgggttcatc tgaaccggga agaatggctt tgttatcccg tcaaggccgg agattttctc
     3961 gcgcatgttt attttagcga gaaatttttc gagctgggcc tttccatggc gaaccttttt
     4021 gaattggcgt cggatgaaga cctcaaacgg aagccggacg cggtttacct gtacggagtt
     4081 gccgaagatc agtttaaggc tcgcgacgta ttcccgaccg ttttttatga ggatgaaaag
     4141 aacgggatgc tggtgggggc ggtccccgcg aaagatgagt ttggttattt cggatatctt
     4201 aaaaaaatga ttttaacgct tcacaatatc aaaaagatcc ggtccgggaa aatgcctttt
     4261 cacggcgctt tcatgaaggt ttttctgaaa ggtaaaaagc ccgcgaccct gctcctgatc
     4321 ggcgattcgg gagccggaaa atcagagacg ctcgaagcga tgcgcggttt tggcgcggat
     4381 ctgatccagg atgtgatcgt tatcgctgat gacatgggat cgttggagat gaaagagtcc
     4441 ggggacgtgg taggatttgg aaccgagacg ggcgcgcttt cgaggctgga tgatttgaag
     4501 ccgggatttg ccctgggaca aattgacagg gctattatca tgaaccctca tgaggtgaat
     4561 gcgcgaatcg cgctaccgat cacgtcgttt gccaacatta ttcaggagca tgccgtggat
     4621 ttcgtgttgt acgcgaataa ctacgaagac atagatgagg atcatccggt gattgagcgt
     4681 ttccagacgg ttgaagaagc tatcaaggtt tttcgcgacg gggcggtgat gagcaaggga
     4741 acgaccaaaa cggtggggct tgtgcacacg tatttttcga acgtgtttgg cccggttcag
     4801 tgcaaggcgg agtatgacgc gatcgcgaag aagttctttg aagctttctt taaaaagaat
     4861 atattcgtag gccagattcg cacgcggctt ggggtgcatg gttgggaacg caagggcccc
     4921 gaagaatccg cggtagcgct tcttgaatta atccggaacg atcaacgata ttgaaccctg
     4981 ggtgcggtgg tctggctcag ggggaagctc tttagcagag cgttaggaga ttatgaccca
     5041 agacgtttat atttgcctcc acgggcactt ttaccagcct ccccgcgaga atccctggat
     5101 tgaagaaatt gaacaacagg acagtgccac gccgtttcat gactggaacg aacgcattca
     5161 ctacgaatgt tatcttccaa actcccgcgc ccgcgccctg gacgccaaag gaaaaatcgt
     5221 cgagatcgtc aacaattttg aacggtcgag ttttaatttt gggccgaccc tcctctcctg
     5281 gcttgaagcg aaacatcccg atacttacaa aagtattatc cgcgccgatc aggtaagccg
     5341 ggagttgcat cgagggcacg gaaacgcgat cgcgcaggtt tttaatcaca tgattatgcc
     5401 tctcgcgaat ctgagggaca agcgcacgca ggtgcggtgg ggactcgagg atttcagaca
     5461 ccgatttggt cgcgaacccg agagtatttg gcttccggaa accgcctgta acgaggacac
     5521 gctcgaagtg ctggtggaag agaagatcaa gtttatcatt ctttccccgt atcaggcgcg
     5581 ggcggtccgg tcgatggaag ggggtgagtg gaaagatgtg acgggggggc atattgatcc
     5641 gcgcaatccc tatcggtgtt ttttaaagaa taatccttcg aagtatctcg atatcttttt
     5701 ttacgacggc ccgatttccc gcgccatcgg gttcgacaac cttctctttg acgcgaaact
     5761 ttttatggat cgccttcaag gcgccgtggt caaggatggc ggtcctcaac tgattcatat
     5821 tgccactgac ggagagacgt acgggcacca taaaccgttt ggagaccggg tgctggggtt
     5881 cctgacttac attgaagtgc ccaaacgcgg attaaaactg acgaattacg gggagtacct
     5941 cgaaaattac cccccgaaaa atgaggtgcg cttgatcgag ggagaaaacg gcgaggggac
     6001 ggcgtggagt tgcgcccatg gagtctcgcg ttggaaagat cactgcggat gccgcggagg
     6061 tgggcccgcg aactggaccc agcattggcg taaaccgtta cgatgggcac tggactggct
     6121 tcgcgatgag ctggcgcggg tctatgaaaa agagggaagt aaatacctga aagatgtctg
     6181 ggcggcccgg gacgattaca ttcggattat cctggatcgg aatgaaaaaa caattcgctc
     6241 ctttttttac aagcacgccc agcgggaact gggtaagacc gaggttaccg cgtgcctcaa
     6301 acttctcgag atgcaacggt atctgatgct gatgtatacg agctgtggct ggtttttttc
     6361 cgagatttca ggcgttgaaa ccgttcaaat ccttcagtac gcggcccggg cggttcaact
     6421 gggcgaagaa ttgagcgggg cgcattatga gagcgaattt ctggagcatc ttgcgctggc
     6481 caagagcaat atcgaaatgt ttaaggacgg acgcggggtt tatgagaagc ttgtgcgacc
     6541 cgcgatggcg actttgcaac acatcgtgag
//
LOCUS       DAOSIO010000085         6565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_10671_length_6565_cov_3.256989, whole genome shotgun sequence.
ACCESSION   DAOSIO010000085 DAOSIO010000000
VERSION     DAOSIO010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10671_length_6565_cov_3.256989"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1193)
                     /gene="ribD"
                     /locus_tag="PK590_04300"
     CDS             complement(66..1193)
                     /gene="ribD"
                     /locus_tag="PK590_04300"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOG23851.1"
                     /translation="MNLETHEKWMGLALRLAARGKYSVSPNPMVGACIVKGDRLISSG
                     YHREFGGPHAERIALERAGRRARGATLYVTLEPCSSWQKTPPCASLILERGIKEVVIG
                     SLDPNPLNHRKGIAYLKKNQIRVVWGILESKVREQNQGFFTLIAQGRPFVTLKMAVTL
                     DGKIATREGKSRWISSALSRNFVHQLRTQQDAVLVGKNTFYLDDPRITVPARARCLKN
                     GRPWKIVLVSDEKIPHKPRIFQDSRMVFFACPRSKMKSILKKMETVPGAFNLLPVEDK
                     NGELDIRDLLGKLGGLGIARLLVEGGGELAWSLIRSGCVDHAVWILAPKIFGGRDAKT
                     SVEGRGVASPDQAYGFIVEKTSKSGQDFIFQGKIEKKDKKE"
     gene            complement(1174..1626)
                     /gene="nusB"
                     /locus_tag="PK590_04305"
     CDS             complement(1174..1626)
                     /gene="nusB"
                     /locus_tag="PK590_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOG23852.1"
                     /translation="MIRKRTQAREYAVQILYRLEINPGGIADSLDEFWSSPEQKDVSR
                     EVRDFAEAIVTGTRNHVAEIDQVVDQYADNWSIDRMAVIDRNILRCATYEILFLVDIP
                     PKVTINEAVNLAKKFSQEESGKFVNGILDKIAHTEKPRVAKADESRNA"
     gene            complement(1623..2141)
                     /gene="ribH"
                     /locus_tag="PK590_04310"
     CDS             complement(1623..2141)
                     /gene="ribH"
                     /locus_tag="PK590_04310"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOG23853.1"
                     /translation="MKIFQGKFHSRKRKIGIVAARFNEYITKRLLEGAVTALHKAGVK
                     DQHIEIVWVPGGLEVPQFCKKLREQRWCDALIAIACVIRGGTYHFEVVSHELTRGISQ
                     VALETGVPIATAVITADHLEQAIDRAGLKAGNKGEQAALVALEIASLDQQLKRKRSNP
                     MNKRLVSKKGET"
     gene            complement(2138..3349)
                     /locus_tag="PK590_04315"
     CDS             complement(2138..3349)
                     /locus_tag="PK590_04315"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOG23854.1"
                     /translation="MRALNSIEEIVQDIRSGRMVIMTDDEARENEGDLIFAAEKVTSD
                     LVNFMMRYGRGLVCVPMASERIEAIGLEDMNQNPEDLMKTAFTVSVDARSGITTGISA
                     FDRTRTIELLASPKSRREDFVIPGHVFPLRAKKGGVLCRAGHTEAAVDLAMLAGLEPV
                     GVICEIINDDGKMARLSDLIPFAKAHGLKIGTIRDLIEYRRRQDKLVRKVSEARLPTK
                     FGEWTIHVYRVLMDGLEHVALVKGTIGVKPVLLRVHSECFTGDVLGSLRCDCREQLHA
                     SMKMIEKEGSGVVLYMRQEGRGIGLGNKIKAYALQDQGLDTVQANEALGFKPDLRDYG
                     IGAQILRDLGLSELRLITNNPRKIIGLEGHGLRVVERIPMEVKANCLNVKYLKTKREK
                     MGHFLELNDKP"
     gene            complement(3734..>6565)
                     /locus_tag="PK590_04320"
     CDS             complement(3734..>6565)
                     /locus_tag="PK590_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23855.1"
                     /translation="VTNYTVETSGLRYTAPIPMVSGDLYDENTLSVLNGTPDLIAGSG
                     NTLVGEPIALMTMTQGSSSEFQYEYDLGPSNTSFTFVSITNGAFVNNVFQGEYLSFPN
                     GKIVIAAKGASGQKVKIEVRDAKQLTATFILELQPYLQNYTLVLSGANVPAGFETQRV
                     ADITIVQDTNIGSSLPFDVVTFETRGLAYAPVPLNPDLAAIRNDLVQSGLDYFRVGAG
                     IDPVTHFPYDNMGENADNPDKLTQPTLIGFYLQILGDVVSGALDNGMTQNQALAEISL
                     VLGNLLNVQRDYGWNGLIPWLSLNPLNASEPKVALGDNANLSQSIAVMIGALEQAGLS
                     AAQLNPIQSQANTFLANQKSGYAAFVDPVFGIFRGDVNTATGVFSAYIDRLANEFRGT
                     VAFLAVYFSDVIPRSVWDNLKIVVNDSYVDRNGETIRNLAPWDGGAFQIFWPMLRNDE
                     LGFSGFRRALYNQLVTQLDYAYQNRIPGILSASLIPEHGYSGAIGIPVISEANMALGT
                     IDIVGDVGSTYALAAAMGVDPYAVLGWLDAIDNLGRVYGGYGFFDSARSNSEISYLYL
                     GIDVAVTILGLAGNGASDFTSYLRNRGLEAAYNELYDSMSRRLGITKANSGFPNAPEF
                     PDRSLAVFSNIASEGAINYTTPPTTELYGVQFSYSHLQKYRGHFWKLKQGYDASANQL
                     RINYSAENSPGYIRIELKNAAGEIVYKTTVSLQEGAKFSSLVIDLPASDLLKNVTEVD
                     LVIDPDDPNGGADADGDFIIHAIQFQHFRSTGKSPGGRSGAVTAAPLLPDPNLTSLNA
                     TVLPSNGGTTPVVSVPEVLPNVYRLEFDVTTPASSTVQIDFDPLNNGSYADFSRLKSI
                     VFGLSSNEANSVKFEIKDSFGNKAGTRVSNVDVSRSYYEFLRSTAEEQGVDMTQVDTI
                     SFTVDSESVPTGDGTGSLELEIGGLQKGT"
BASE COUNT         1694 a         1812 c         1409 g         1650 t
ORIGIN      
        1 gtccgccgcg attctccttt tttatcacaa ccccttgatt ttcaataata ccggtaaaca
       61 tcccatcact cctttttatc ttttttttca atcttgccct gaaaaataaa atcctgtcct
      121 gatttcgaag ttttttccac aataaatcca tacgcctggt ccgggcttgc cacgccccgt
      181 ccttcaactg aggttttcgc gtccctgcct ccaaaaatct tgggcgccag aatccagacc
      241 gcgtggtcca cacaaccgga tctgataagg gaccaagcca gctctcctcc cccttcaaca
      301 agcaacctcg cgattccaag tcccccaagt tttcccaaaa gatcccgaat gtccagctct
      361 ccgtttttgt cttcgacggg caaaagatta aaagcgcccg gaaccgtctc catttttttt
      421 aaaatggatt tcattttgga tcgtggacac gcaaaaaaaa ccatccggga atcttgaaaa
      481 atacgcggct tgtggggaat tttttcgtca gaaaccagga caatcttcca cggtcgaccg
      541 tttttgagac aacgcgcgcg ggcgggaacc gtaatccgcg gatcatccag ataaaaagtg
      601 ttctttccca ccaggaccgc gtcctgctgt gttcttaact ggtggacgaa gttacgggac
      661 agggctgaag aaatccatcg ggatttccct tcacgcgtgg caattttacc gtccagggta
      721 accgccattt taagtgtcac aaacggacgt ccttgagcta tcagggtaaa aaaaccctgg
      781 ttctgttccc tcacttttga ttcaaggatc ccccagacta cccgtatctg attcttttta
      841 agatacgcga tacccttacg atggttgagg ggattcggat caagtgaacc aatgaccacc
      901 tccttgattc ctctttcgag gatcagggaa gcgcagggcg gggtcttttg ccaggatgaa
      961 cagggttcaa gtgtaacata aagagtcgcg ccccgggcac gccggccagc ccgctccaaa
     1021 gcgattcgtt cagcgtgggg gcccccaaac tcgcgatggt atcccgatga aatcaatcga
     1081 tcccccttga cgatacaggc tccgaccatg gggttaggac ttacactgta tttcccccgg
     1141 gcagcgagcc gcagggcaag gcccatccac ttttcatgcg tttcgagatt catcagcctt
     1201 ggcgactctt ggtttttccg tatgcgcgat tttatcgaga ataccattga cgaatttgcc
     1261 cgattcttcc tgtgaaaact tttttgccag attgactgct tcattgatcg taactttggg
     1321 agggatatcc accaaaaaga gaatctcgta agtcgcgcat cgaagaatgt tcctgtcaat
     1381 cacggccatc cggtcaatgg accagttatc tgcgtactgg tcaaccacct gatcaatctc
     1441 cgctacatga ttccgggttc ccgtcacaat cgcttcagca aaatcacgga cttctcgcga
     1501 cacatctttt tgttccgggg aactccaaaa ttcgtctagg ctgtccgcga taccccccgg
     1561 gttaatttcc agacgataaa gtatttgaac cgcgtattcc ctggcctggg tgcgctttcg
     1621 gatcatgttt cccctttttt agaaacgagt ctcttgttca tcgggttaga ccgttttctt
     1681 ttcagctgtt ggtccaagct ggcaatttca agagcaacca gagccgcctg ctcccctttg
     1741 ttccctgcct taaggccagc ccggtcaatc gcctgctcaa gatgatcggc cgtaatcacc
     1801 gccgtcgcga ttggaacccc cgtttcaaga gcgacctgtg aaatccctct cgtcaattcg
     1861 tgcgagacca cttcaaagtg ataggttccc ccacggatca cacacgcgat cgcaattaag
     1921 gcgtcacacc accgttgctc ccgaagcttt ttgcaaaact gtgggacttc caatcctccg
     1981 ggaacccata caatttcgat atgctggtct ttcactcccg ctttatgaag cgccgtcacg
     2041 gcaccctcaa ggagccgctt cgtaatgtat tcgttgaaac gagctgcgac aattcctatt
     2101 tttcttttcc gggaatgaaa ctttccttgg aaaattttca tggcttgtca tttagctcca
     2161 ggaaatgccc catcttttcc cgctttgttt tgaggtattt cacgttaagg caattcgctt
     2221 taacctccat cgggatcctc tcaaccaccc gtaatccgtg cccctcaagc ccaataattt
     2281 ttcgaggatt atttgtaatc aaccgaagtt ccgaaagacc aagatcccgc aaaatttgcg
     2341 cgccaatgcc ataatctcgg agatcgggtt taaaccccag cgcctcattc gcctgaaccg
     2401 tgtccagacc ttgatcctgg agagcgtagg cctttatctt atttccaagg ccaatgccgc
     2461 ggccttcctg tcgcatataa aggacaacgc cacttccttc tttttctatc attttcatgg
     2521 acgcatgtaa ttgttcccga caatcgcacc ggagcgaacc gagaacgtcc ccggtgaaac
     2581 attccgaatg aacccgcaaa agaacaggct tcaccccaat cgttcctttg acaagcgcca
     2641 cgtgttccaa accatccatg agaacccggt agacatgaat agtccattcc ccgaatttag
     2701 ttggaagacg cgcctcactt accttacgca ccaacttgtc ctgacgcctg cgatattcga
     2761 taagatcccg aatggtgccg attttcagcc cgtgggcttt tgcaaaagga atcagatccg
     2821 aaagacgagc catttttccg tcatcgttaa tgatttcaca aatcacgcca acgggttcca
     2881 gtcccgccaa catggcaaga tccacggccg cctccgtatg tccggcccga cacagcactc
     2941 cccctttttt tgcgcgcaac ggaaacacgt gcccggggat cacaaaatct tctcgtcggg
     3001 atttcggact ggcaagcagc tcaatcgtcc gggtccggtc aaaagctgaa atgcccgtcg
     3061 taattcctga tcgagcgtca accgaaactg taaaagccgt tttcatgagg tcttctggat
     3121 tctggttcat gtcttccagg ccgatcgcct ctattctttc ggatgccatc ggtacgcaga
     3181 ctaagccacg gccgtatctc atcataaaat taacgagatc cgacgtcacc ttctcagccg
     3241 cgaaaatcag atccccctca ttttcgcggg cctcatcgtc ggtcataatg accattcgac
     3301 cggaacggat atcttgaact atttcctcta ttgaatttaa agcgcgcata ggattgatgt
     3361 tgttctgtta ctttgaagag taaatttggc tcatcgtagc agtcccgctc aaatttgtca
     3421 aacaagggat gccataactc gtttaatgat aatgtgttgc aaagattgga acagtgcctg
     3481 ccttgacggt tgcccgaaga gatgctacct taggttcaga ggagctggct gtttgtcttt
     3541 gacaatttgg tcaccccgaa agatggggac gaaagctaaa agccccagcg tgagaaactt
     3601 accgtggcct aaaagcgttt cgcgtacaaa gatggtcttt gtgactatga ttttaaaata
     3661 gtaagcggga cgatcaacct cacagctggg gctttttgtt tctatcgtta cctgggtaag
     3721 atttgaatcg aaactaggtt cctttctgca aaccaccaat ctccaactca aggctacctg
     3781 tcccgtcccc ggttgggacc gattcagaat cgaccgtgaa tgaaatagta tcgacctgcg
     3841 tcatatcaac cccctgctcc tcggccgtcg atcgcaaaaa ttcataataa ctccgcgaca
     3901 catccacgtt cgatacacga gtaccagcct tattcccaaa agaatctttg atctcaaact
     3961 ttacagaatt ggcttcattt gagcttagtc caaaaacaat gctcttgagg cgtgaaaaat
     4021 ctgcgtagct cccgttattc agcggatcaa aatcaatttg taccgtggaa gaagcgggag
     4081 ttgtcacgtc aaattccaac cgatacacat tgggcaggac ttcggggaca gaaacaaccg
     4141 gtgtagtccc gccattcgaa ggaagtaccg tagcattcaa actggttaaa ttcggatccg
     4201 gtagtaaagg agccgccgtc acagcgccac tacgaccccc tggcgatttc ccggttgatc
     4261 ggaaatgctg aaattgaatc gcgtggatta taaaatctcc gtctgcatcg gccccaccgt
     4321 tgggatcgtc gggatcaatg accaaatcca cttcggtaac attcttcaaa agatcagagg
     4381 ccggcaaatc aatcacaagg ctcgaaaact tggctccttc ctgtaacgac accgttgttt
     4441 tataaacaat ctcccccgca gcattcttca gctcaatacg aatatacccg ggagaatttt
     4501 cagccgagta attaatccga agctgattgg cactggcatc gtatccctgt ttaagcttcc
     4561 agaaatgccc ccggtacttc tgaagatgac tgtaggaaaa ttgcacacca tacaactcgg
     4621 ttgtcggagg cgtggtatag ttaattgccc cttcggatgc aatattacta aacacggcaa
     4681 gggagcgatc aggaaattcg ggcgcattcg ggaaacccga atttgctttg gtaattccca
     4741 gtcggcggct catggaatcg tagagttcgt tataagccgc ctccaatccg cgatttctaa
     4801 gatacgacgt aaaatcggaa gctccgtttc ctgctaaccc cagaatagtt accgcaacat
     4861 caatccccaa ataaagataa ctaatttcag aattggatcg cgccgaatcg aagaagccgt
     4921 agcctccata cacgcgcccc aggttatcaa tcgcgtccag ccagccaagc accgcataag
     4981 gatcaacccc catcgccgcc gccagcgcat acgtcgaccc gacatccccc acaatgtcaa
     5041 tagttcctaa agccatgttg gcttcggata taacgggaat ccctatcgcg cctgaatatc
     5101 cgtgctccgg gattaagctc gcggaaagaa ttccgggaat acgattctgg taagcataat
     5161 ccagttgtgt cactaattga ttgtaaagag cgcgccgaaa tccagaaaaa ccaagttcat
     5221 catttcgaag catcggccag aaaatctgga acgcgcctcc atcccaaggg gcaagatttc
     5281 ggatggtttc accgttgcga tccacataac tgtcattgac cacgatctta aggttatccc
     5341 aaaccgagcg gggtatcaca tctgaaaaat acaccgccag gaaagcaaca gtaccccgaa
     5401 actcatttgc cagtcgatca atatacgcag aaaacactcc cgtagcagta ttcacatcac
     5461 cgcggaagat tccaaatacg ggatccacaa aagcggcata gccacttttc tgattagcta
     5521 aaaacgtatt cgcctgactt tgaatcggat ttaattgggc ggcagataga cccgcttgtt
     5581 ccaacgcccc gatcattaca gcgatacttt gagacaaatt cgcgttatcc cccaacgcaa
     5641 cttttggttc actagcatta agcggattaa gactgagcca ggggatcaaa ccattccagc
     5701 cataatctct ttgaacgttt aataaatttc ccaagaccag agaaatctcg gcaagggcct
     5761 gattctgggt catgccgtta tcaagagcgc cgctgaccac atcgcccagt atctggagat
     5821 aaaaaccaat cagtgtgggt tgggtgagct tgtcaggatt atcagcattt tcgcccatat
     5881 tgtcataagg gaaatgggtc acgggatcaa taccggctcc caccctgaaa taatccaatc
     5941 cggattgaac gagatcattt cggatcgctg caagatccgg attcagcgga acgggcgcat
     6001 aagccaatcc ccttgtttcg aaggtcacaa cgtcaaaagg gagcgacgat ccgatattag
     6061 tatcctggac gattgtgatg tcagcaactc tctgcgtttc aaaccccgcg gggacatttg
     6121 cgcccgacaa aaccagcgtg taattttgaa gatacggctg gagttccaaa ataaaagtgg
     6181 ccgtcagctg tttcgcatcc cgaacttcta ttttcacttt ttggcctgaa gcccccttcg
     6241 ccgcaatcac aattttccca tttggaaatg acaaatactc tccctgaaaa acattattaa
     6301 caaacgctcc gttcgtaata ctcacaaagg taaacgatgt attgctcggg ccaaggtcgt
     6361 actcgtactg aaattcactc gacgacccct gagtcattgt catcagggca atcggctctc
     6421 ccaccaaagt gttcccgctt cccgcgataa ggtccggcgt tccattcaaa accgaaagcg
     6481 tattctcatc atacaaatcc cctgacacca tcggtatcgg ggccgtatac ctcagacccg
     6541 aagtctcaac cgtgtaattc gtcac
//
LOCUS       DAOSIO010000086         6507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_10777_length_6507_cov_4.634377, whole genome shotgun sequence.
ACCESSION   DAOSIO010000086 DAOSIO010000000
VERSION     DAOSIO010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10777_length_6507_cov_4.634377"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..116
                     /locus_tag="PK590_04325"
     CDS             <1..116
                     /locus_tag="PK590_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HOG23856.1"
                     /translation="DVIREGREVTYDLKTDRSDPSAAGTREMGQAIIRKMK"
     gene            187..1113
                     /gene="mdh"
                     /locus_tag="PK590_04330"
     CDS             187..1113
                     /gene="mdh"
                     /locus_tag="PK590_04330"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOG23857.1"
                     /translation="MPKAKVTVVGAGFVGATTAQRIVEKDLADVVLVDIVEGMPQGKA
                     LDIMESAPIEGFSAKITGTNRYEDTAGSEVVVITAGLARKPGMSRDDLLFKNAEIVGG
                     IAAECAKHSPEAVLILVTNPLDVMTWLAREKSGFPCERVLGMAGELDSARYAYFVAER
                     LHCQPSEVKAMVLGGHGDQMVPVPEHTTVKGTPITKLMSAADLEAINQRTRDGGAEIV
                     KLLKTGSAYYAPSASAVKMVRAVLNDAGEVIPSCVYLTGQYGLKDVYCGVPAALGRGG
                     VKKVIELAITDSQRAALQASASDVRLNFEKLT"
     gene            1161..3860
                     /gene="acnA"
                     /locus_tag="PK590_04335"
     CDS             1161..3860
                     /gene="acnA"
                     /locus_tag="PK590_04335"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HOG23858.1"
                     /translation="MNLLNSFGSKVTLNIAEKDYTVYRIRALQECGVAKVLKLPYAIR
                     VLLENLLRNEDGKNVTRHDIENLANWSPAKKSGKEIAFTPARVLLQDFTGVPAVVDLA
                     AMREAMVKMGGDPKKINPIQPVDLVIDHSVQTDFFGTPDSLRKNVEKEFERNRERYQF
                     LKWGQKAFSNFRVVPPATGIIHQVNLEYLSPLVFRRKEKGKTYLYPDTLVGTDSHTTM
                     INGLGVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFKLTGKIKEGVTITDVVLTITQML
                     RQKGVVGKIVEFFGPGVSFLPLADRAVISNMAPEYGATAGYFPADDETIRFMRLSGRA
                     GELIKVAESYLKEQGLFRENPAEDIQYSDTLELDLKTVDMSLAGPRRPQDRVALGEVK
                     TSFEKALPELLKGKSDPDKKISIEVDGQKCELEHGAVVIASITSCTNTSSPSAMLTAG
                     LLAEKAALKGLSVKPWVKTSLAPGSQAVTDYLRDSGLLYYLEKMRFHLAGYGCMTCIG
                     NSGPLPDPVAWAVREGDMVVASVLSGNRNFEGRVHAQVKANYLASPALVVAYAIAGKV
                     TVDLEQSPLGTDKEGNPVFLKDIWPSQDELSEAMKSVKPEMYRHRYKNVFDGSESWKA
                     LLSPDGDTFEWDKKSLYVKHPPYFQKMPARPAEVRNIRDARALAVLGDSITTDHISPA
                     GAIRPDSPAGKYLIANGVEPKDFNSYGARRGNHEVMVRGTFANIRLKNLMVPGIEGGM
                     TKYFPEKGRPVDTTIYEASLKYQERKVPLIIVAGKEYGSGSSRDWAAKGPALLGVEAV
                     IAESFERIHRSNLIGMGILPLQFIDGENANTHKITGEELFSIEGISGELSPGKVLTCK
                     ALTGGDRKHPSEFKVICRIDTPVELEYYKHGGILQSVLRGLLE"
     gene            3997..5166
                     /locus_tag="PK590_04340"
     CDS             3997..5166
                     /locus_tag="PK590_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOG23859.1"
                     /translation="MMSNIKPEITKEPSSASKLIKGLEGVVAAQTSLSDVDGENGRLL
                     FRGIPIGELVEKSHFEEVVYLLWYGKLPTSIELETFKETLKAQRPLSDRAMEILKLIP
                     TQAHPMSALRTMCSLMGSLDPRAHIVNPEESQRKAYHLTGVFPTIIAAFFRLRVGKEP
                     IAPDMNLSQAANFLYMMTGEKPAPEMEKALDAYLILLADHGLNASTFAARVTTGTLSN
                     LYSAVTSAVGTLAGELHGAANQHAMEMILEIGAADKAASYVNRLLDEKKKVMGFGHRI
                     YKTRDPRAEAFREIANNLCVKAGKTEWLEIAGIVESVMMERKKIPCNVDFFSAPVLCV
                     LGFPPDFFTTVFAASRVAGWSAHIIEQQLDNRLIRPLAEYVGPQDAYYVPAKDRK"
     gene            5210..>6507
                     /locus_tag="PK590_04345"
     CDS             5210..>6507
                     /locus_tag="PK590_04345"
                     /inference="COORDINATES: protein motif:HMM:NF015683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HOG23860.1"
                     /translation="MAANLDYAFDEYLKARPSESVSAVQSFHEKLRHTTFRFGRFTIP
                     AFYKAFFVTPKQHHLLKRVAAAFHQIINTTTRLYFEEPQLANRFHLAPEAEDLVRIDP
                     GYSRNVVFGRFDALLEGESLKILEFNCDTPIGASYADQLETAFLSEKLVAPFAEENHL
                     AASERVQNILNSLLAVYEEFGGYETPQIAIVDWRHARTHAESEFMKAHFETRGYKSVI
                     ADPRELQYRGGKLYHKGFRVHLIYRRVHFDTLVERLDEVQDLIRAYRDKAVCIVNPLR
                     AHLSSNKSIFSLLTNRAFDRFFTDSENQMKKNHLPWSRCVADAEDFYGHRKVFLVDFL
                     KDEKETLVLKPPRSHGPEHVRIGRETPDGDWNAAVDKALKEPGWVIQEYVNVPVVTVP
                     QVVNGKLDFAYKKHNFNMLVFGGKYSGGLVRLSDESVVNVA"
BASE COUNT         1522 a         1650 c         1941 g         1394 t
ORIGIN      
        1 cggacgtgat ccgtgagggc cgtgaggtga cgtatgatct taaaacggat cgcagtgatc
       61 cgtccgcggc tggaacgcgc gaaatgggac aggcgattat caggaaaatg aaataagtac
      121 ggcatgcggc gcgcgacgcg tggcggtagt ctgccccgct cgccttacat tttgaaagga
      181 actaatatgc cgaaagcaaa agtaacggtc gttggcgcgg gatttgtcgg cgcgacgacc
      241 gcgcaaagga ttgttgaaaa agatttggcg gacgttgttt tggtggacat cgtcgaaggg
      301 atgccccagg ggaaagcgct ggacattatg gagtcggcgc ccatcgaggg gttttccgcg
      361 aaaataactg gcacgaatcg gtacgaggac acggcggggt cggaggtggt ggtgatcacg
      421 gccgggctcg cgcggaagcc gggcatgagt cgtgatgacc ttctttttaa aaacgcggaa
      481 atcgttggag gcattgccgc ggagtgcgcg aagcattctc ccgaagcggt gcttatcctt
      541 gtgacgaatc cgctggacgt gatgacgtgg cttgcccggg aaaagtcggg gtttccttgc
      601 gaaagagttc tcgggatggc gggtgaactt gattcggcgc gttacgctta ctttgtcgct
      661 gaaaggcttc actgtcagcc ttcggaagta aaagcgatgg tgctgggcgg acatggggat
      721 cagatggttc cggtgccgga gcacacgacc gtgaaaggga cgccgatcac gaagctgatg
      781 agcgcggccg atcttgaggc gatcaaccaa cggacgcgtg acggaggagc ggaaattgtg
      841 aaactgctca agacgggaag cgcttattac gctccgtcgg cttccgccgt caaaatggtg
      901 cgggcggttt tgaatgatgc gggagaagtg atccccagtt gtgtctatct gaccggacag
      961 tacgggctga aggatgttta ttgcggagtg cccgccgcgc tgggccgggg cggtgttaaa
     1021 aaagtcatag aactcgcgat cacggattcc cagcgggcgg ctttacaagc gtcggcttcg
     1081 gatgtccgtc tcaactttga aaaattaacg taaaatcccg ggcgggctgg gacggggccc
     1141 gggcgggggg aaaaagggac atgaacctct tgaacagctt tggaagcaag gtgacgctga
     1201 acatcgcgga gaaggactac accgtttacc ggattcgcgc gctccaggag tgcggggtcg
     1261 ccaaagtttt gaaacttcct tacgccatcc gggttttgct ggaaaacctc ctccggaatg
     1321 aagacggtaa gaatgtgacc cgtcacgata tcgaaaatct cgccaactgg tctcccgcga
     1381 agaaaagcgg aaaagaaatc gcgttcacgc cggcgcgcgt gcttttgcag gattttacgg
     1441 gagtgccggc cgtggtggat ctcgcggcga tgcgcgaggc gatggtaaaa atgggagggg
     1501 atcccaaaaa gatcaatccg atccagcccg tggatctcgt gatcgatcat tcggtccaga
     1561 cggacttttt cgggacgccg gactctttgc ggaaaaacgt tgagaaggag tttgaacgaa
     1621 accgggaacg ctatcagttt ctcaagtggg ggcagaaggc tttctccaat tttcgggtgg
     1681 tccctccggc gacggggatt attcaccagg tgaatctgga atatctttcg ccgcttgttt
     1741 tcaggcgcaa ggaaaaaggt aaaacctacc tgtacccgga cacgctcgtc ggcaccgatt
     1801 cgcacacgac tatgatcaac ggcctggggg tgctcggctg gggggtcggg gggattgagg
     1861 cggaggccgc gatgctgggc cagccggtct cgatgctgat ccccgaagtg gtcgggttca
     1921 aattgaccgg aaagataaaa gaaggcgtga cgatcacgga cgttgttttg acgattaccc
     1981 aaatgcttcg ccaaaaaggt gtcgtcggaa aaatcgtgga attttttggc ccgggcgttt
     2041 cctttctgcc gctggcggac cgggccgtga tttccaacat ggcgcccgaa tatggggcca
     2101 cggcgggcta ttttccggct gacgacgaga cgatccgttt tatgcggctg agtggacgag
     2161 cgggtgaact catcaaagtc gcggaatctt acctgaaaga acaaggtctt ttccgggaga
     2221 acccggccga agatatccag tactctgata cgctcgagct ggaccttaaa acggtcgaca
     2281 tgagtctggc cggaccccgc cggccgcagg accgggtcgc gctgggagag gtgaaaacct
     2341 cgtttgaaaa agcactgccg gaacttttga aagggaaaag cgatcccgat aaaaaaattt
     2401 cgatcgaagt cgacggacaa aaatgcgaac ttgagcacgg cgccgtcgtg atcgcctcga
     2461 tcacgagttg cacgaacacc tcctctcctt ccgcgatgct gaccgcggga cttctcgcgg
     2521 aaaaggcggc gctgaaggga ctgagcgtca aaccctgggt gaaaacaagt ctggccccgg
     2581 gctcgcaagc ggtgacggac tatcttcgcg acagcggtct tctttattat cttgagaaaa
     2641 tgcggtttca tctggccggc tacggatgca tgacttgcat cggaaactcg ggccccctcc
     2701 cggacccggt ggcgtgggcg gtcagagaag gggacatggt ggtggcctcc gtgctttccg
     2761 gcaaccgaaa ttttgaaggg cgcgtgcacg cgcaggtcaa ggcaaactat ctcgcgtcgc
     2821 cggccctggt ggtggcttac gcgatcgccg gtaaggtgac ggtggatctg gagcaaagtc
     2881 ctctgggaac cgacaaagag ggcaatcccg ttttcctcaa agacatctgg ccgtcacagg
     2941 acgaactttc cgaggccatg aaaagcgtga agccggagat gtaccgccac cgctacaaga
     3001 atgtctttga cgggagcgag tcctggaagg cgctcctttc gccggacggt gacaccttcg
     3061 aatgggacaa aaaatccctt tacgtcaaac atcctccgta ttttcagaaa atgccggccc
     3121 gtcccgccga agtccggaat atccgggacg cgcgggcgct ggcggtgttg ggcgactcga
     3181 tcacgaccga tcacatttcg ccggcagggg cgatcaggcc cgacagcccc gcgggaaaat
     3241 atctgatcgc gaacggagtg gaacccaaag attttaattc gtacggcgcg aggcgcggca
     3301 accatgaagt gatggtacgg ggaactttcg cgaatattcg cctgaagaac ctgatggttc
     3361 cggggatcga aggtggcatg acaaagtatt ttcctgaaaa agggagaccg gttgatacca
     3421 cgatctatga agcttccctg aaatatcagg aacggaaagt cccgctgatt atcgtcgcgg
     3481 gaaaagaata cggctccggc tcctcgcggg actgggcggc caaaggcccc gcgcttcttg
     3541 gcgtggaggc tgttatcgcg gaaagcttcg aacgcatcca ccggagcaat ctgatcggga
     3601 tggggattct tccgctccag tttattgatg gggaaaatgc gaacactcac aagatcacgg
     3661 gagaggagct gttttcgatc gaaggcattt ccggcgagct gagccccgga aaagttttaa
     3721 cgtgtaaggc tctgacaggg ggagaccgga aacatccgtc ggaattcaag gttatctgcc
     3781 ggatcgacac cccggtcgaa cttgaatatt acaaacacgg cggtattctc cagtcggttc
     3841 tccgcgggct tctggaataa aaacagtttc atgagcccat ttttcaattc cgccaatgtt
     3901 atctccggtc gcgttcaacg gaccggattt ttctttgtgt ttaccggatg cttttcagta
     3961 taatgctgaa acttttaaca tttaaaacag gatctcatga tgtcaaacat aaaacctgaa
     4021 attaccaaag aaccctcaag cgcctccaaa ctgatcaagg ggctggaagg cgtggtggcc
     4081 gcgcagacct cgctcagcga cgttgacggg gaaaacggcc ggcttctctt tcgggggatt
     4141 cccatcgggg aattggtcga aaagtctcat tttgaggaag tcgtttatct gctctggtac
     4201 ggaaagcttc cgacaagcat tgagcttgaa acttttaagg aaaccctgaa ggcccaacgc
     4261 cccctttcgg accgggcgat ggaaattctg aagttgatac ccacccaggc gcacccgatg
     4321 agcgcgctca gaacgatgtg ttcgttaatg ggatcccttg acccgcgcgc ccatatcgtg
     4381 aatcccgaag agtcccagcg aaaggcctat catttaacgg gggttttccc gacgatcatc
     4441 gcggctttct tccggcttcg cgtggggaag gagccgatag cgccggacat gaacctgtcg
     4501 caggccgcca atttccttta catgatgacc ggtgaaaaac ccgctcctga gatggaaaag
     4561 gcgctggatg cctatcttat ccttcttgcc gatcacgggc tcaacgcctc caccttcgcg
     4621 gcgcgcgtga ccacgggaac gctttcgaac ctttattccg ccgtcacttc cgccgtgggc
     4681 accctcgcgg gtgaattaca cggcgcggct aatcagcacg cgatggagat gatccttgaa
     4741 atcggggcgg cggacaaagc ggcctcttat gtcaatcgtc ttctcgacga gaagaaaaaa
     4801 gtcatgggat tcgggcaccg gatttacaag acccgggatc cgagggccga ggcgtttcgc
     4861 gagatcgcga acaatctctg cgtcaaggcg gggaaaaccg agtggctcga gatcgccggc
     4921 attgtcgaat ctgtcatgat ggaacggaaa aaaattccct gcaatgtcga ctttttttcg
     4981 gcgccggtcc tttgcgtatt gggatttccg ccggattttt ttacgacggt ttttgcggca
     5041 agccgcgtgg cggggtggag cgcccatatc attgaacaac agctcgataa ccggctcatc
     5101 cgtccgcttg cggaatatgt cgggcctcag gacgcgtatt acgttcctgc gaaagatcga
     5161 aaataagaca agttttgctt tgtgaaacca tccgagggtt gtgaggggca tggcagcgaa
     5221 tctggactac gcgtttgacg aatacctcaa ggcaaggcct tccgaatctg tttcggccgt
     5281 ccagtctttc cacgaaaaac tgaggcacac cacgtttaga ttcggacgct ttacgatccc
     5341 cgccttttac aaagcttttt ttgtgacgcc gaagcaacat cacctgctca agcgcgtagc
     5401 ggcggcgttc catcagatca tcaataccac gacgcgactt tattttgaag agccccagct
     5461 tgcgaatcgt ttccaccttg ccccggaagc cgaagacctt gtccggatcg accccggata
     5521 ttcccggaac gtggtttttg gaagatttga cgcgttactc gagggggaaa gcctcaagat
     5581 ccttgaattc aactgtgaca cgccgattgg ggcctcttat gccgatcaac tggaaacggc
     5641 gtttctttcg gaaaaactgg tggcgccgtt tgccgaagag aaccacctcg cggcgagcga
     5701 acgggtccag aacattttga actcgctttt ggcggtttat gaggaattcg gcgggtacga
     5761 aaccccgcaa atcgcgattg tggactggcg gcacgccaga actcacgcgg aaagcgagtt
     5821 tatgaaagcc cactttgaga cccggggata taaaagcgta atcgcggacc ctcgcgaact
     5881 tcagtacagg ggcggcaaac tctatcacaa gggatttcgc gttcatctca tttaccggag
     5941 ggttcatttc gatacgctgg tggaacggct ggacgaggtc caggacctga tacgcgcgta
     6001 ccgcgataaa gcggtttgta tcgtcaatcc cctgcgggcc catctttcgt ccaacaaatc
     6061 gattttttcc cttttgacga atagggcatt tgaccggttt ttcacggaca gtgaaaatca
     6121 gatgaaaaaa aaccatctcc cctggagccg ttgcgtggcc gatgccgaag atttttacgg
     6181 acaccgcaag gtttttctcg tcgattttct caaagacgaa aaagaaacgc tggtgctcaa
     6241 acctccgcgc agtcatggtc ccgagcatgt gcgaatagga cgcgagactc cggatgggga
     6301 ctggaacgcc gcggtggaca aggccctcaa ggaaccagga tgggtcatcc aggaatacgt
     6361 gaatgtcccg gtggtgaccg ttccccaggt ggtgaacgga aagctggatt tcgcttacaa
     6421 aaagcacaat tttaatatgc tggtatttgg gggaaaatat tcgggagggc ttgtccgcct
     6481 gagtgacgaa agcgtggtca atgtcgc
//
LOCUS       DAOSIO010000087         6503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_10784_length_6503_cov_4.025900, whole genome shotgun sequence.
ACCESSION   DAOSIO010000087 DAOSIO010000000
VERSION     DAOSIO010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10784_length_6503_cov_4.025900"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="PK590_04350"
     CDS             complement(<1..1333)
                     /locus_tag="PK590_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23861.1"
                     /translation="MFQKGCSFSVFFLIVGAGPALAALGASVTVSPGQPTDIYPGETT
                     QLEITLSNYNELAAITAVAFSNNLPGTLPNGLKINGAPTYTCTDPSDGSVVAGSGVLT
                     AVNGTQAISLAGGVIPARANNTDGTCSIKIPVTGGSSTGNAATYTYTIANGAVTGNDG
                     SPVANLGAVSQSVNIRAVSIPTISKTFGTNTLILGGAPTKLTLTVTNPNPTVSIPNFS
                     ITDNFPRSGGQAIIKVADTPNATSTCTGTGVAATFNPNPGDTSITATGGTVAPNGSCT
                     VTVQVAANWTNGVHQTSLLNNTIAGATDFTNDLGLVPADASAQIRAQSPLNISKTFAH
                     STMASGSSDTVRITFSNTGTTPLTITSFTDDPIDGVDNPSFGLLINGPITASCTGAGT
                     AGSYVATGDNEGFSQTSNTTIAAGGSCTITVPFTGTVKSANTPVSFTNTIAQ"
     gene            complement(1806..2903)
                     /locus_tag="PK590_04355"
     CDS             complement(1806..2903)
                     /locus_tag="PK590_04355"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOG23862.1"
                     /translation="MKSGKIRKIGILTAGGDCPGLNAVIRAATKTAIQAYGWSVFGIE
                     DGYAGLIEGRGHELTYEAVSGILVQGGTILGTSNRANPFRTPVVEGRKVRYVSRTKEA
                     LRNFREWGLDALLTIGGDGTLSIAHKLSKLGVPVVGIPKTIDNDLSGTDFTFGFDSAV
                     TIATEAIDRLHTTAAAHHRIMILEVMGRHAGWIALHAGSAGGADVILIPEIPFSMKRV
                     RDAVLRRARYGKRFSLVCVAEGAREKGGKVIVQKHDLSNPYPEKLGGVGEYLAERIEK
                     SCGLETRAAKLGHVQRGGSPTSFDRNLGTLFGAYATELVSEKRFGEMVCLQGMKVRSI
                     PILDAVKNLKTVPPDHYLIQTGESIGVCFGR"
     gene            complement(2908..3360)
                     /locus_tag="PK590_04360"
     CDS             complement(2908..3360)
                     /locus_tag="PK590_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HOG23863.1"
                     /translation="MMKRAMIFFIITGMCFSGCATESGLTERETGGLAGAALGAGIGA
                     LAGSATGHAGVGTAIGAGAGLLTGAVAGEMARRQKEQTKKELREEMMQQQYQPMYAPQ
                     PYTATQPVATAPVARQELNTKYNPRTGQTFPSTYTYDPNTGEALKTLQ"
     gene            complement(3458..4108)
                     /locus_tag="PK590_04365"
     CDS             complement(3458..4108)
                     /locus_tag="PK590_04365"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HOG23864.1"
                     /translation="MAIVKKLLITAGPTREHLDPVRFITNLSSGRMGYTLAGVAAAKG
                     YQVSLVSGPTHLKVTRGVQYYPVISSHEMKRVCSKLFPSHDSLIMTAAVCDYMPSTTD
                     RHKRSSIGGLTLKLKRTPDILADLSKRKGDRIVIGFCLETRSLVQRAKEKVRQKKLDG
                     IVANFYSPRKQVPFGDNKVRAILIDRDMKVIREPMQNKTSLSWRILRWMEKLNYER"
     gene            complement(4120..4707)
                     /locus_tag="PK590_04370"
     CDS             complement(4120..4707)
                     /locus_tag="PK590_04370"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HOG23865.1"
                     /translation="MSKSSPRAKRLSSKTVLQIVTGSIAAYKTPDLVKMMKEDGAKVI
                     CVQTDSSRKFVTETVLRTVTGERVYSDLFPNETPWGVMHTSLADLADVILIAPASANF
                     IAKLAHGIADDLGTCVCLATRRPILIAPAMNDHMYTHPLTQRNMAQLKAIGYRFVDPV
                     EGDLACGRTGVGHIAPLATILESLVSVLKTGKKSK"
     gene            complement(4670..4891)
                     /locus_tag="PK590_04375"
     CDS             complement(4670..4891)
                     /locus_tag="PK590_04375"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOG23866.1"
                     /translation="MAYIALEKILPQSKDASLYKTVLIAATRANELAQGAQPLVKTES
                     KKAATVALLEMAAQKVKYVEVKPKSKKTL"
     gene            complement(4893..5495)
                     /gene="gmk"
                     /locus_tag="PK590_04380"
     CDS             complement(4893..5495)
                     /gene="gmk"
                     /locus_tag="PK590_04380"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOG23867.1"
                     /translation="MSNKTRQTQGLLVVLSAPSGCGKTSVVERLLIRHQDWMRSVSTT
                     TRSPRLGEENGTHYHFISKESFELEKGRDTFLEWAEIYGQYYGTPRQHVIDAVDSGKV
                     MILTIDVQGTKQIKESWGTERPMISVFLLPPSVKALRERLTNRNTETPEEIERRIMIA
                     QDEIKAATLYEKTVINQNLDQAVLEVESIILNELKKRSEK"
     gene            complement(5485..6351)
                     /locus_tag="PK590_04385"
     CDS             complement(5485..6351)
                     /locus_tag="PK590_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOG23868.1"
                     /translation="MTAFSRVVLTAGGKQWAVEIKSLNQRYFELGLRLPPALLPYETQ
                     VRRLVQGMIRRGKVSLAVSEEGNGEQRLSYELDRAQVKAYLDSAATLKKKFRFEGRLL
                     LADVLRLPGVVREKVNTRAKTLSWPEFQRILVKGIERTISLKREEGRKLAKDIRERLE
                     KISRTTDSIEKLVKQKQSECFVKVSRRIREILGDEKMDQDRMAREVAFLADKSDITEE
                     LVRMRSHLKLFTKWLSQAGEFGREMDFLCQEMMREANTMASKAQMFEVSKEAIAVKSE
                     IEKIREQVQNVE"
BASE COUNT         1523 a         1822 c         1543 g         1615 t
ORIGIN      
        1 cctgggcaat ggtattggtg aaagagaccg gggtattagc ggacttcacg gtccccgtaa
       61 aaggaacggt gatggtacac gaccctcccg ccgcaatcgt agtgttgctg gtttgagaaa
      121 atccttcatt atcgcccgtc gccacataac tgcccgccgt gccggctccg gtgcaactcg
      181 cggtgatcgg tccattaatg agaaggccga agctcggatt gtcgaccccg tcgatcggat
      241 catctgtaaa cgacgtaatg gtgagcgggg tcgtacctgt attcgagaag gtgatgcgca
      301 ctgtgtcgct tgagccactc gccatggtcg agtgcgcgaa tgttttactg atgttaagcg
      361 gtgactgggc gcgaatctgg gcggaagcat ccgcggggac caaccccaga tcgttcgtaa
      421 aatcggtcgc ccccgcaatg gtattgttga gcaaagacgt ttggtggacg ccattggtcc
      481 agtttgcggc cacctgaaca gtaaccgtgc aactgccgtt cggcgccacg gtgcctccgg
      541 ttgccgtgat tgatgtatca cccgggttag ggttgaatgt tgccgcaaca ccggtccccg
      601 tacaagttga agtggcattc ggcgtatcgg ctactttgat gatggcttgt cctccagacc
      661 gagggaaatt atcggtaatg ctgaagttcg gtatcgagac cgtgggattc gggttggtga
      721 ccgtcaaagt cagttttgtg ggcgctcctc ccaatattag tgtatttgta ccgaatgttt
      781 tgctgatcgt cgggatcgag accgcccgga tattaacgct ttgactgaca gcgcccaaat
      841 tagcgaccgg tgaaccatca ttgcccgtga ccgctccgtt tgcgatcgtg taagtgtagg
      901 tcgccgcatt gcccgtcgat gacccccctg tcacaggaat tttgatggaa cacgtcccat
      961 ccgtgttgtt cgcccgagcg gggatcacac ccccagcaag cgaaatggcc tgcgtgccgt
     1021 tcactgccgt aagcactccg cttccggcga caacgctacc gtcactggga tcggtacaag
     1081 tataagtcgg cgctccgttg atctttagtc cgttgggaag cgtcccggga agattgttcg
     1141 agaatgcaac cgctgtaatc gccgccagtt cattataatt cgaaagagtg atctcaagct
     1201 gagtggtttc gccggggtag atatcagtgg gctggccggg ggaaacagta acggatgctc
     1261 caagggcagc caacgccggg ccagcaccga cgatgaggaa aaaaacagaa aaagaacagc
     1321 ctttttgaaa caaaatttct tgagatcaaa cagctcttcc atcaataatt tccacccttg
     1381 cgtgcgtatc ggcgcaacaa agcccgttat gctcccggaa tcggagctcc tcgcccacct
     1441 ttattttaca tatgaaaaaa ggctttgaag ctccacagcg aatgaatcat caagagcctt
     1501 tcttcataaa gtcttacaaa tcccaaactc caggaatagc aaaaaatttt aatttttata
     1561 ttaacataaa atcatacccc ctcctaatga agatttactc attttctcat cgctcaactc
     1621 gtgtggtaaa gatcaaaaaa ctttgaagta gagccaggca gaataaaaca aaagcgacaa
     1681 gacgagcggg gaaaggagtt tggtgggtgt tacaacaggc tagaaaaagc ttctggcagg
     1741 ttttgcggac cgatacatta ccactttgct accgcgccgg gaattcacat caaaccgaaa
     1801 aacttttagc ggccaaaaca gaccccgatg gattcacccg tctggataag ataatggtcg
     1861 ggcggaacgg ttttcaaatt cttgaccgca tccaggatcg gaatcgaacg aaccttcatc
     1921 ccctgcaagc agaccatttc gccaaatcgc ttttcagaaa cgagttcggt ggcataagcg
     1981 ccaaaaagag tcccgagatt ccggtcgaaa gacgtggggg aaccgccgcg ctgaacgtgt
     2041 ccaagttttg ccgcacgggt ttcaagacca caactttttt cgattcgctc cgccaggtac
     2101 tctcccactc cgcccagttt ttcaggataa ggattcgaga gatcatgttt ctgcacaatg
     2161 accttccccc ctttttccct ggcgccttcg gctacacaaa caagactgaa ccttttcccg
     2221 tagcgggccc gtctgagcac ggcgtcccga acccgtttca ttgaaaacgg gatttcaggg
     2281 atcaggatca catcggctcc acccgccgaa cccgcgtgca aggcaatcca gcccgcgtga
     2341 cgtcccatga cttccaggat cataatacga tgatgagccg ccgcggtcgt atgcaaccgg
     2401 tcaatcgcct cagtggcaat cgtcaccgcc gaatcaaacc cgaaggtgaa atctgtcccg
     2461 cttaaatcgt tatcgatggt tttgggaatt ccaaccacgg ggactcccaa cttggaaagc
     2521 ttatgcgcga tggagagagt tccatcacct ccaatggtca gaagcgcatc cagtccccat
     2581 tcacgaaaat tgcggagagc ttccttcgtt cgactcacgt atcgaacctt ccggccttcc
     2641 accaccggag tccggaaagg attcgcgcga ttggatgttc caaggatcgt cccgccctgg
     2701 acaagaatac ctgaaacagc ctcataggta agttcatgcc cccgaccttc aatgaggccc
     2761 gcgtacccgt cctcaatacc aaacacgctc catccgtacg cctggatggc cgttttggtg
     2821 gcagcacgaa tcaccgcgtt caaccccgga caatcccctc ctgccgtcaa tatcccgatt
     2881 ttacggattt ttcctgattt cactcgatta ctgtagcgtt tttaacgctt ctcccgtatt
     2941 aggatcatac gtatacgtgg aagggaaagt ctgcccggtg cgcgggttgt atttcgtatt
     3001 caactcctgt cgcgctaccg gcgcggtagc aacgggctgg gttgccgtgt acggctgagg
     3061 cgcgtacatc ggctgatact gctgttgcat catttcttcc cgaagctctt tcttcgtttg
     3121 ctctttctgg cgacgcgcca tctcaccggc tacggctccg gtcagcaaac cggctcccgc
     3181 cccgatcgcc gtacccacgc ccgcatgtcc cgtagcgcta ccagccaaag ctccaatacc
     3241 ggccccaagc gccgcacccg ccaatcctcc ggtctcacgc tccgtcaatc ctgactccgt
     3301 cgcacaaccc gaaaagcaca tccctgtgat gataaaaaat atcatggctc gtttcatcat
     3361 gttcgcgtct ccttgtttga aatatttcgc agagcgaaaa ttgaggggtt aattgaaggg
     3421 gtattataat cgttttcctg tttcaaaaaa cagtttttta cctttcgtag ttcaattttt
     3481 ccatccatct caaaattcgc cacgacaggc tcgtcttatt ttgcatcggt tcacggatca
     3541 ctttcatatc ccggtcaatc aagatagcgc ggactttatt gtctccaaaa ggcacctgct
     3601 ttcgtggaga ataaaaattc gcgacaatgc cgtcgagttt tttttgcctg accttttcct
     3661 tcgccctttg aacaagactc cgggtctcaa ggcaaaaacc gatgacaatc cggtcccctt
     3721 tccttttcga cagatccgcc agaatatcag gcgttctctt cagtttaagc gtcaatcccc
     3781 caatactcga tcgtttgtgc cggtcagtcg tcgacggcat gtaatcgcaa accgccgccg
     3841 tcatgatcag gctatcatga gaaggaaaaa gtttcgaaca aacccgtttc atttcgtgag
     3901 aggaaatcac cggatagtac tgaacgcccc ttgtcacttt aaggtgggtc ggtccactca
     3961 ccagactcac ttgataccct ttcgcggccg cgactccagc caaggtatac cccatccttc
     4021 ccgaagaaag gtttgtaatg aaacgaacgg gatcaaggtg ttcacgggta gggccggctg
     4081 tgatcagaag tttttttact atcgccatgg caaggggaat tatttagatt tcttaccggt
     4141 tttcaaaacc gaaacgagag attccaggat cgtcgccaac ggcgcgatat gccccacacc
     4201 agttcgtccg cacgccaaat caccttcgac aggatccacg aaacggtacc cgatcgcctt
     4261 caactgcgcc atgttgcgtt gagtcagggg atgtgtatac atgtggtcat tcatggccgg
     4321 cgcgatcaag ataggccgac gggttgcgag gcaaacacag gttccgaggt catcggcgat
     4381 cccgtgtgcg agcttcgcga taaaattggc cgaggctggc gcgatcaaaa taacgtcggc
     4441 aagatccgcc agagacgtat gcatgacccc ccagggagtc tcattcggaa acagatcgga
     4501 ataaactctt tcaccagtca ccgtccggag cacggtttcc gtcacgaact tcctggaaga
     4561 atccgtctgc acacaaatca ccttcgcgcc gtcttctttc atcatcttga ccagatcagg
     4621 cgttttgtac gcggcaatgc ttccggtgac aatctggaga acggtcttgc tagagagtct
     4681 ttttgctctt gggcttgact tcgacatact ttactttctg cgcggccatt tcgagaagcg
     4741 ccaccgtcgc cgcctttttg gattccgtct tcacaagagg ctgggctccc tgggcaagct
     4801 cgttcgcccg ggtagccgct atcagcacgg ttttgtaaag actggcatct ttactttggg
     4861 gaagtatttt ttcaagtgcg atatacgcca tattactttt cgctcctttt tttcagttcg
     4921 ttaagaataa tcgattcaac ctcaagaacg gcctgatcaa gattctgatt gatcaccgtc
     4981 ttctcataga gcgtcgcggc cttaatctcg tcttgcgcga tcatgatccg ccgctcaatt
     5041 tcctcgggtg tttccgtatt acggtttgtc agtctttccc tgagcgcctt gacagacggc
     5101 ggaaggagaa aaacactgat cattggccgt tcagtccccc aagattcctt gatttgtttg
     5161 gtcccctgaa catcaatggt caaaatcatg accttcccgc tgtccaccgc gtcaatcaca
     5221 tgttggcgcg gagttccgta gtactgccca taaatttcag cccattccag gaacgtatcg
     5281 cggcccttct caagctcaaa cgattctttc gaaataaaat gataatgggt gccgttttcc
     5341 tcgcccaggc gcggagaacg cgtcgtggtg gaaaccgacc tcatccagtc ctgatgacga
     5401 atgagaaggc gttcgacgac actcgttttc ccgcatcccg aaggcgcgga caataccaca
     5461 agcaaacctt gcgtctggcg ggtcttattc gacattctgg acctgttcgc gaattttttc
     5521 gatttcgctt ttgaccgcga tcgcttcctt cgagacttca aacatctgcg ccttggacgc
     5581 catcgtattt gcctcccgca tcatttcctg gcaaagaaaa tccatttcgc gcccgaattc
     5641 tcccgcttgg gaaagccact tggtaaaaag cttcagatgg cttcgcatcc gtaccaactc
     5701 ctcggtaata tcgcttttgt cggccaaaaa agcaacttcc ctcgccatcc gatcctgatc
     5761 catcttttca tcccccagga tttcccggat cctgcggcta accttcacaa aacattcgga
     5821 ttgtttctgc ttcacaagtt tttcgattga atccgtggtt cgcgaaatct tctccagcct
     5881 ctcgcgaata tccttggcaa gtttacgtcc ttcctcacgt ttgagggaaa tcgtcctttc
     5941 aatgcctttg accaggatgc gttgaaattc aggccagcta agcgttttcg cgcgagtatt
     6001 caccttttcc cgcaccactc cgggtaaccg cagaacatcc gccagcagaa gccgtccctc
     6061 aaacctgaac ttttttttca gagttgccgc tgagtcgaga tacgctttga cctgcgcgcg
     6121 atccagctca tacgaaagac gctgttcacc gtttccctct tcagaaaccg ccagcgacac
     6181 cttgccgcga cggatcattc cctggaccag ccggcgaacc tgtgtttcat acggtaacaa
     6241 cgcgggcggc aatctcaaac caagctcaaa atagcgttgg ttcagcgatt tgatctcaac
     6301 tgcccattgt ttccccccgg cggtgaggac cacgcgggaa aaagctgtca tgctcttaat
     6361 cataaaatcg gggtaaaaag gaaatttaac atggtggagc gtgtattata agaggccctc
     6421 tacctctgtc aagggaaagc ctttccaatc gagttgactc atattaaatc ttaccataga
     6481 gcgatcattg gccgaatcgt tga
//
LOCUS       DAOSIO010000088         6419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_10945_length_6419_cov_3.799026, whole genome shotgun sequence.
ACCESSION   DAOSIO010000088 DAOSIO010000000
VERSION     DAOSIO010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10945_length_6419_cov_3.799026"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..6404)
                     /locus_tag="PK590_04390"
     CDS             complement(12..6404)
                     /locus_tag="PK590_04390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG23869.1"
                     /translation="MMRSAAASPSGAKPSKVATSSAVAMSPKAAMSHKSFKAFQKGLQ
                     ALGWYKGKVDGKDGPLTRSAVRTLQTILRDELGFSKLPVNGQYDDPATLAAIKGLQGK
                     LKVKVDGVLGPETASSLKKHLEELRKKGRETQAVTGQGVSEVLPPHFPSDPATEVLNN
                     PTLQAGFPALGFQVIQRLLVSRQLVPNTQETRNFIYTYLYETHQIVQTAKQTLTDAQF
                     QRVFHSKKSEMTPERVAQLAIFVFDLNNHESMGHVHIKNKRSSAFSQAQILIGTRKEL
                     FKNPLFKEITGITGAQPVTEKEWEEFLVTGSSANRNRIAMVIIHFLRAPRDLPFDELF
                     SGDTVKESNMWGKYYHTQSPKGPMEAFKKKADRHKDDGNRAKQYYLSQVRGKGSSPKD
                     AVRAASGARSELRQGDIQTAEVRPEGLRDESKYFVSEIRRLISYIGFFNTASIAALSG
                     ISFSMIRFMFQRLPDLKESIHLLSAIPLSILPEAQSGAVFLLGAVFFAGLSSILSGHF
                     GLKVFDALLSRRISLEETERRGQMMFDEIYRESLTKDSALAKAMEKTDGVFATPVSYD
                     VFEGGVETTVDEAGRESFKIRLSPATTISKSILRYLIVRRSIGIDFRLKTRALRQKAG
                     NSPLFDLVSNNFWWGYYGESQTLRRWRYFPVMALLYEIPLTIGLSSFAPIFLLSILSA
                     DFLTGNQTVVKFLRSMVSVFVHGRDVDEIEFQGEPREKLVFGSLRKAFAMWSGIWGIV
                     EGIFAGLVFFATMNLQNLSRTGWVPPVWNLLGFDWNLLHAMFICGSSLGIAFMWMAYL
                     VYPNEKTRSEEVVEKPKKRSEVRDEGYAASGARSELRQGDIQTALSRSEARGLVSFSE
                     TPVDWDKVFTVKGPLVVEVGFGDGDILAAESEQHPQWRHVGIELPFDDMMRKGENPSL
                     DHLMGKDNVRVILHDARESVAKMFEDGTVAQFRFFYPDPWVGFFDKGCPLRQEVWKKL
                     KPGGSIILVTENVGYAESVRKVFSDQGFEAWREEVEIFDFVDGAEERRARQLSGIPIK
                     KSRYWEMYAPSDDSHAWVVKAVKPDTRSETRGLVSFSETPVDWDKVFTVEGPLVVEVG
                     CGGGGILAAESEQHPQWRHVGIELPFDDMMRKGENPSLDHLMGKDNVRVILHDARESV
                     AKMFEDGTVAQFRFFYPDPWVGFFDKGCPLRQEVWKKLKPGGSIILVTENVGYAESVR
                     KVFSDQGFEAWREEVEIFDFVDGAEERRARQLSGIPIKESPYWQRLTASDDSHARVVK
                     AVKPKILLEERAEESLFEYDVRIRDSKLPEKIPSDFVILSPVFNEQDQLPGVLENIKR
                     RKYSEDVIFVDDGSSDGTAEILRASGVYFISFAQNARKESALLNAMAVLRKHHHRVPE
                     YFVAVDGDSYLAGTGQGTLRAELEKSIQEMEKGGYVARPIDIEAHLTARSGTLEWLQG
                     IRWYLTMGICAVYSKISFPSPSFWGGGNIFKTQAFLDVVAKRKEDFESGDVELNGIAG
                     SMGKVGKPLSTVKVRTEALKKWDDFIKQQRRWARAQFEGTPSWFFAGFLSGLVLFDVF
                     ILLNLPAFMALMMVSVIMSVLFAALRHKVTQSQKFASTDSSLPPKNIYLRTDVSDARS
                     EIRGDEFDASDYGLMRGRLSRDSERTLKSLTEYFSRNQIAGDDANLIARTLVRMKPRS
                     LELLRDWGVLTEKEPELITSGNMGKIMIPQDIRNSLAQRMRLLLKSYDERGYTRAQKD
                     VLMSRLIPVDQIDAILTRSPDLTDGIVIEMLDSRFSLIFQIDRAIEVAASLRKSGVVP
                     SLAWRILIKQGPDQALSWASAAKMVVERIKDAQEVSGQRNAWLMIMNHGIEKVEKAER
                     QGKLSELMNAWKTRSELRQEGILEGLKEKIQETYGRVGIYGEHLSMENILLKVLEDVG
                     RENNLALVEGEDFELFWYRERFGIIIINPAYKKTDEVKDKIIERLQFYYRKYKPKIKL
                     TTEKRSDGVEASYWITPASLTEFGKFFGTADIQYYDQEHRFPRPFTLKEMREKLLHKA
                     EMLQEGMRRSGLPPAEIEKFGQWASDVDFALANSDRYGQEEMIRTESIMRSRGRRDSS
                     IGLHEDGYRYTTLNAMKENAPPRPMIAVGCEGRRNRFARCCQTLLSCVSWKFTSMK"
BASE COUNT         1688 a         1829 c         1295 g         1607 t
ORIGIN      
        1 ttgtctacgg atcacttcat tgatgtgaat ttccaggata cacaggacag caaggtctgg
       61 cagcatctcg cgaacctgtt gcgcctgcct tcgcacccca cggcgatcat tggcctgggc
      121 ggagcatttt ccttcatggc attcaaggtc gtatacctgt atccgtcctc atgcagtccg
      181 atagaagaat ctcttcgacc tcggctacgc atgattgact ccgtccggat catctcctcc
      241 tgtccatacc tgtccgaatt ggcaagcgca aagtcaacat ctgatgccca ctgcccgaat
      301 ttttcgattt cagcaggcgg gagcccacta cgtctcatac cttcctggag catctccgcc
      361 ttgtgaagaa gtttctcccg catctctttc aacgtaaaag gccttggaaa tctgtgttct
      421 tgatcataat actgaatatc cgccgtcccg aaaaactttc cgaactccgt cagtgaagcc
      481 ggagtaatcc agtaagaagc ttcaacccca tcggaacgtt tctcagtcgt taacttgatt
      541 ttaggcttat acttacgata ataaaattga agtctctcaa taatcttgtc cttgacctcg
      601 tccgtttttt tataggcggg atttattatg ataattccaa aacgctcccg ataccaaaaa
      661 agctcaaaat cctctccctc gaccagcgcc aaattattct ctctgccaac atcttccaga
      721 actttaagga gaatattttc catggacaaa tgttctccat aaatccccac cctgccataa
      781 gtctcttgaa tcttctcttt cagtccttcg aggatgcctt cctggcgaag ttcggagcgt
      841 gttttccatg cgttcatcaa ttcagaaagt ttcccttgac gctcggcctt ttccactttt
      901 tcaataccgt gattcatgat catcaaccaa gcatttcttt gcccgctcac ttcttgagcg
      961 tccttaattc tttcaacaac catcttcgcg gcagaggccc aggacaatgc ctggtcaggc
     1021 ccctgcttga tcaaaatcct ccaggccagg ctaggaacaa cgcccgattt ccgcagactt
     1081 gccgcaactt caatggcgcg atctatttga aaaatcagag aaaaacggct gtccagcatt
     1141 tcaatcacaa tgccatcggt aagatccggg cttcgggtca aaattgcatc gatctgatca
     1201 acgggaataa gccgcgacat gagaacatcc ttttgtgccc tggtgtagcc ccgttcgtca
     1261 taagatttca gaagaagtcg cattctttgg gccagggaat ttctaatatc ctgggggatc
     1321 atgattttcc ccatattccc agaggtaatc agttcgggtt ctttctctgt gagaactccc
     1381 caatcacgga gcaattccag cgaccggggt ttcatacgaa ccagtgtccg agctatcagg
     1441 ttcgcgtcat ccccggctat ctggtttcgg gaaaaatatt ccgtaaggga cttcagcgtt
     1501 ctttctgaat ccctgctcag tctccccctc attaagccat aatcactggc atcaaactca
     1561 tccccacgga tctcggagcg tgcatcggaa acatcggttc gcaaataaat gtttttcgga
     1621 ggcaacgatg aatccgtact ggcaaatttc tgagactgcg taaccttgtg gcgcaacgcg
     1681 gcaaaaagaa cagacataat caccgacacc atcatcaggg ccataaatgc cggaagattc
     1741 aataaaataa acacatcaaa taaaacaaga cccgacaaaa agcccgcaaa aaaccagctt
     1801 ggggttcctt cgaactgggc acgagcccac cttctttgtt gcttaataaa atcatcccac
     1861 ttttttaaag cttcagttct gaccttaacg gttgaaaggg gtttccccac tttgcccatc
     1921 gaaccagcaa tcccatttaa ttccacatcc cccgattcga aatcttcttt tcttttcgca
     1981 accacatcca aaaaggcctg ggttttaaaa atatttcccc caccccaaaa actcggagag
     2041 ggaaaagaaa ttttcgaata aaccgcgcag atccccatcg ttaaatacca gcggatcccc
     2101 tgaagccatt cgagagtccc tgaacgggcg gttaaatgag cttcaatatc aatcgggcgg
     2161 gccacatacc cacccttttc catctcttga atcgatttct ccagctcagc gcgaagggtc
     2221 ccctgtccgg ttccggccag ataagaatct ccgtctaccg ccacaaaata ctcgggcacc
     2281 ctatggtggt gtttccgcaa aactgccatc gcattcaaaa gagcgctttc cttccgtgcg
     2341 ttttgagcaa aagaaataaa ataaacaccg gaagcccgca gaatctccgc cgtcccatcg
     2401 cttgaaccgt catcaacaaa aatcacatcc tccgaatatt tccgccgttt aatattttcc
     2461 aaaactcccg gaagttgatc ctgctcatta aaaaccgggg acagaatcac aaaatcagac
     2521 ggtatttttt cgggcaactt cgaatcacga atacgaacat cgtactcaaa caagctttcc
     2581 tcggccctct cctccaacag gattttgggt ttaaccgctt tcaccaccct agcatgcgaa
     2641 tcatccgatg cagtcaaccg ttgccagtaa ggagactcct tgatgggaat acctgacagc
     2701 tgtctggctc ttctctcttc tgccccatca acaaaatcaa aaatctcaac ctcttctctc
     2761 caggcttcaa atccctggtc gctgaatact ttcctgacag attctgcata ccctacattc
     2821 tcagtaacca ggataatgga acctcccggt ttcaattttt tccacacctc ctgcctcaac
     2881 ggacagcctt tatcaaaaaa acccacccac ggatcagggt aaaaaaaccg gaactgcgcc
     2941 acagttccat cttcaaacat tttagcaacc gattcgcggg catcgtgcaa aatcacccgc
     3001 acattgtctt tccccatcaa atggtcaagc gacggatttt cacctttccg catcatgtcg
     3061 tcgaaaggca gttcgatccc gacatgacgc cattggggat gttgttcgga ttccgcggca
     3121 aggatgcctc cgcccccaca cccgacttcc acaacgagcg ggccttccac ggtaaaaacc
     3181 ttgtcccaat caacaggcgt ttcagaaaag gaaacaagcc ctcttgtctc cgagcgggta
     3241 tccggcttta cagctttcac cacccaagca tgcgaatcat ccgatggagc atacatttcc
     3301 cagtagcgag acttcttgat gggaatacct gacagctgtc tggctcttct ctcttctgcc
     3361 ccatcaacaa aatcaaaaat ctcaacctct tctctccagg cttcaaatcc ctggtcgctg
     3421 aatactttcc tgacagattc tgcataccct acattctcag taaccaggat aatggaacct
     3481 cccggtttca attttttcca cacctcctgc ctcaacggac agcctttatc aaaaaaaccc
     3541 acccacggat cagggtaaaa aaaccggaac tgcgccacag ttccatcttc aaacatttta
     3601 gcaaccgatt cgcgggcatc gtgcaaaatc acccgcacat tgtctttccc catcaaatgg
     3661 tcaagcgacg gattttcacc tttccgcatc atgtcgtcga aaggcagttc gatcccgaca
     3721 tgacgccatt ggggatgttg ttcggattcc gcggcaagga tgtcgccgtc cccaaacccg
     3781 acttccacaa cgagcgggcc tttcacggta aaaaccttgt cccaatcaac aggcgtttca
     3841 gaaaaggaaa caagccccct cgcttcagaa cgagacagcg ccgtctggat atcgccctga
     3901 cgaagttcgg agcgcgcacc ggatgccgca tatccctcat ccctcacctc cgaccgtttt
     3961 ttaggtttct ccacaacctc ctcactccgt gtcttttcat ttggatagac aaggtacgcc
     4021 atccacataa aagcaatccc gagagaactc ccgcagatga acatagcatg tagaagattc
     4081 cagtcaaaac ccagaagatt ccagaccggg gggacccaac ccgtgcggct caaattctgc
     4141 aaattcattg tggcaaagaa aacgagtccg gcaaagatcc cctccacgat gccccaaatt
     4201 ccggaccaca tggcaaacgc ctttctcagg gatccgaaaa ccagtttctc gcgcggctcc
     4261 ccctggaatt cgatttcatc gacgtcacgg ccgtgaacaa aaacactcac catcgacctc
     4321 aggaatttca ccactgtttg attcccggtc aaaaaatccg ccgagagaat cgaaagcaaa
     4381 aaaatgggcg cgaaactgga taggccaata gtaagcggaa tttcatagag cagggccatc
     4441 acagggaaat accgccaccg tctcagcgtc tgactttcgc cgtaataccc ccaccagaaa
     4501 ttattcgata caagatcaaa aagcggagaa tttcccgctt tttgtcttaa cgcacgcgtc
     4561 tttaaccgaa aatcaatgcc gatggaccgc cgtacaatca gataacgcaa aatcgatttg
     4621 gaaatagtgg tggcaggaga caggcggatt ttaaacgatt cacgtcccgc ttcatcgacc
     4681 gtggtttcca cacctccttc aaaaacatcg taagaaaccg gtgtggcaaa aacaccgtct
     4741 gtcttttcca ttgccttggc cagcgccgaa tccttggtta gactctcgcg ataaatttca
     4801 tcaaacatca tctgccctct tctctccgtc tcttccaatg agatacgcct ggacaagagg
     4861 gcgtcaaaaa ctttcagccc gaagtggccg cttaaaatcg acgacaggcc cgcaaaaaag
     4921 accgcgccta aaagaaaaac tgcaccactt tgagcttccg gcaaaatact caacggaatc
     4981 gctgataaca gatggatcga ttccttcaaa tccggaagac gttgaaacat aaagcgaatc
     5041 atcgaaaatg atattccgct taatgccgcg atcgaagccg tattaaaaaa gccaatgtaa
     5101 cttatcagcc tacgaatctc cgaaacgaaa tacttggatt catccctcag cccttcgggt
     5161 ctgacctctg ccgtctggat gtcgccctgg cgaagttcgg agcgcgcacc ggacgccgca
     5221 cgaacagcat ccttgggcga acttcccttc cccctcacct gtgacaaata atattgtttc
     5281 gctcggttcc cgtcatcttt atgacgatcc gccttctttt tgaaagcctc catcggtcct
     5341 ttaggcgact gggtgtggta atactttccc cacatgttcg attctttcac agtatctccc
     5401 gagaacaatt catcaaaagg caagtcacgg ggagctctta aaaaatgaat tatcaccatc
     5461 gcgatgcggt ttcggttcgc ggaagagcca gttacgagga attcttccca ctccttttct
     5521 gtcacaggct gagcgcctgt aattcccgtt atttctttaa acaaggggtt tttaaaaagt
     5581 tcttttcgag ttcctatcaa gatttgagct tgggaaaaag cgctactccg tttgtttttg
     5641 atatgaacat gtcccatgct ctcgtgatta ttcagatcaa aaacaaatat cgcgagttgg
     5701 gccacccgtt ccggagtcat ttcgcttttt tttgaatgaa aaacacgttg aaattgagcg
     5761 tccgtcagcg tttgtttagc cgtctgaact atttgatggg tctcatacag ataggtgtag
     5821 ataaaatttc tggtttcttg cgtgttcggg acaagttgcc gagataccag taaccgctgg
     5881 atcacctgaa aacccaaggc agggaaacct gcctgcaagg ttggattatt caaaacttcg
     5941 gtggccggat ccgatggaaa atggggcggc agaacttcag acaccccctg cccggtaact
     6001 gcttgggttt ctcttccttt tttgcgaagt tcctccaggt gtttcttgag ggaagacgcc
     6061 gtttccgggc ctaaaactcc gtccacttta accttcaact ttccctgcaa tcctttaatc
     6121 gccgccagtg tcgccggatc gtcatattgc ccgttgacag gaagcttcga aaatcccaat
     6181 tcatcacgaa gtatagtctg gagtgtcctg acagccgacc gcgtcagggg tccatccttt
     6241 ccgtcaactt ttcctttata ccaaccaagg gcctgaagtc ctttttgaaa ggctttaaaa
     6301 cttttgtggg acatcgcggc tttgggggac atcgcaaccg cggaagacgt cgcaaccttg
     6361 gagggcttcg cgccactggg tgacgcggcc gcggagcgca tcatcgcaat ggaaacgat
//
LOCUS       DAOSIO010000089         6407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_10959_length_6407_cov_3.512280, whole genome shotgun sequence.
ACCESSION   DAOSIO010000089 DAOSIO010000000
VERSION     DAOSIO010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10959_length_6407_cov_3.512280"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..1605)
                     /locus_tag="PK590_04395"
     CDS             complement(106..1605)
                     /locus_tag="PK590_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOG23870.1"
                     /translation="MNTRKVLMLKDTVLFAAANYVAQGIGILNSFLLRNFMGPAGMGV
                     WSVIQVILGYCGYASLGTTRALARDYPVLRGQKNFKQADRIKNSTLTFSLVMSMVPAV
                     LLLGFVAIQYRKLEKAFLAGLLFLSGFLFVQRFYDFLLTLLRSEKKFRILSEQIIVNA
                     AGGLLITVLVVRPWGIYGLYAGTALFTVCLIVFINRRNPYRFRLSWDKQVLTHELKLG
                     LPLVLSTFLYTFLLGLDKLLLAKKMGFLGVGLYSIAMMVSNYVLSLPMIFSNVLYPNL
                     LESYGEKKGDVSQITGYLKKPIFTLSILVPFLCSLAFAFMPLLVELFLKKFVGGIGSM
                     KIYLVGSFFLLMGQFSNNFLVTIDKYLRIVPVLVASIIINYFANQVFLDRGFGVEGVA
                     LGTCLSFSVYGISTYFLALHAIHGTVTGIRLVIQSVLTYGTFLILIFFLDHLIVMGSS
                     VYLALTKCFVFLLVSTPFLYYLEKREHLISTIWSIVVNRTPFGGRGYDS"
     gene            complement(1609..2187)
                     /locus_tag="PK590_04400"
     CDS             complement(1609..2187)
                     /locus_tag="PK590_04400"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOG23871.1"
                     /translation="MGPGCRILNGMKVQGGKKVTIGRNCFIGRNVMLSAVSGELRIGN
                     NVEIRDNVRIFAKKIIIGDHVTIGEGSFLNGFMEISSGAWISRNCDLTGKIRIERAIL
                     GPKTAIISGDHKRDPQTGEILMPGEGNGGMVVIEKGAWTGYGTIILKGVVIKNNMVVG
                     AGSVVTKSFDEGDVVAGNPAKKINSRLSISKN"
     gene            complement(2285..4156)
                     /locus_tag="PK590_04405"
     CDS             complement(2285..4156)
                     /locus_tag="PK590_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23872.1"
                     /translation="MSLATAVFLDRYSPSYLIQGSSILSLDPRVSRQLDAKGCNYRVL
                     EDLYSEKDLRNREPEFFKDQLKWFTRFDLFLQEGIPLCREYKIPLATASYLRLKYCVD
                     SVLINAVILDQVLPRLKKEGVGEIVYLGNDLTAGESYSVFKFKNRNRWVFQQILALAC
                     NKHGLSFRPQCFSAEPKTETTLLPVKQSFFKHLAKDLLCFFKYRKYVRRKELAAHKAI
                     FLHVGSPDIDIPLKELFGRGMNILVQETDGIINENQLFRPRIPAPELNREETHAIKNA
                     CMLAAGALGENNELIRWFLVQSDLDVTSVIRPFLKGFVEIDAPEVLVQAIKIGRFLST
                     HGIGYVFARGNTDKASLGTLLGAKYMSDAKSVCIEHASFVADNEIFGVMDTATYHQTF
                     ARDEVSECFLVRSLRERYRTDCAVARSTHYLDCVRRLNGRGCSALGLKTVVYVEKKFS
                     DRIRCFNNMNYPLTWYFSFQKKMIDIFASRPDYHFIYKHANSPNQQWAKDSTLVYLKE
                     KNAPNISIFSGSFLESLKMADRVILDGSSGALFEAASAGKSVLCLYSDYLRIIPEAGE
                     IFKDSLKPFSDFYDVEIAVTAFLDSNADTFRPSLQLDDKSFADIFQKTCLARENRHE"
     gene            complement(4251..4940)
                     /gene="pseF"
                     /locus_tag="PK590_04410"
     CDS             complement(4251..4940)
                     /gene="pseF"
                     /locus_tag="PK590_04410"
                     /EC_number="2.7.7.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid cytidylyltransferase"
                     /protein_id="HOG23873.1"
                     /translation="MRSIAIIPARGGSKRIPRKNIKDFLGQPIIAYSIASALASHVFD
                     EVMVSTDDAEIAGISEKCGAKVPFFRSAELSNDRAMTAPVLVEVIKEYKKSGKRFDHV
                     CCIYPCAPFIKEECLRQAMALLTEKRCDSVFPVVKFSYPPQRCLVIRDGKVLMLHPEN
                     YNVRSQDLEPYYHDVRQFYCVRTELLLATGKLIGDNSYPLIIPESEMQDIDTEEDWKI
                     AEMKYRILNHV"
     gene            complement(4937..5950)
                     /gene="pseI"
                     /locus_tag="PK590_04415"
     CDS             complement(4937..5950)
                     /gene="pseI"
                     /locus_tag="PK590_04415"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002850575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HOG23874.1"
                     /translation="MQRKTFIIAEMSANHCGRIDLARKIISEAKHCGADAVKLQTYTA
                     DAMTIDCKDEIFKISGGTLWDGKYFYGLYREAHTPWEWQKELKEYADSVGIEFFSTPF
                     DRSAVDFLESLGVARYKIASFEAVDYPLVKYAASKGKPIIISVGVSEFDEIQEVVDIC
                     KSVGNHDITILKCTSAYPAKLEDMNLLTVEDMKRKFRPQGVKIGLSDHTMNIETVVAG
                     VALGATVIEKHFTLDRSLGGADASFSLNPEEFKRMVDAVRNTEKLLGKIDYSVNERNR
                     KFARPLFVVEDIKRGDRFTAANIRSIRPGVGMHPKHYEEVIGKVARKDLMRGEPLKNE
                     DIA"
     gene            complement(5940..>6407)
                     /locus_tag="PK590_04420"
     CDS             complement(5940..>6407)
                     /locus_tag="PK590_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009752645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOG23875.1"
                     /translation="DLLKLNKQNIVVHGSALPYGKGMSPVSWQILEGKNRIPLTLFEA
                     TERIDAGDIYFQDELRLTGDELIKEWQEKLGLKIVEMCLLFIQKRDGLTARRQVGEST
                     YYRKRIPIDSELDIRKTLAHQFNLLRIVDNDKYPAFFIYKNRKYLLRIEKDAT"
BASE COUNT         1868 a         1623 c         1312 g         1604 t
ORIGIN      
        1 aggcttctat caccggcgcc attttcagcg cctccggccg cgtcccaaaa acaaacaata
       61 ctatcttttt cataaacaac ctctcatcag tgaccctgct cagcgtcaac tgtcgtaacc
      121 tctcccaccg aacggcgtcc gattaacaac aatactccaa atagtcgaga tcaaatgctc
      181 acgtttctca agataatata aaaaaggcgt cgaaaccagc aggaagacaa agcacttagt
      241 caaggccaaa taaacactcg aacccataac gatcaaatgg tcgagaaaaa aaattaaaat
      301 aaggaaggtc ccgtaagtca gcacactttg aatcactagc cgaatccccg ttacggtacc
      361 gtgaatagca tgtaacgcca aaaaataagt cgaaatccca taaaccgaaa aactcagaca
      421 cgttcccaga gcaacccctt caacgccaaa tcctcgatca agaaaaactt gattcgcaaa
      481 ataattaatg ataatggagg caacaagaac agggactatt cgcaaatatt tgtcaatcgt
      541 tacaagaaag ttattcgaaa attgccccat taaaagaaaa aaagagccca ccaaataaat
      601 cttcatactg cctatcccgc caacaaactt cttgagaaaa agctccacca ataagggcat
      661 aaacgcaaaa gccaacgaac aaagaaaagg cacgagaatc gagagagtaa agatgggttt
      721 ttttaaatag ccagtaatct gagaaacatc ccccttcttc tcgccatagg attctaaaag
      781 gttgggatag agcacgttgg aaaatatcat cggcagagat agaacgtagt tactcaccat
      841 cattgcgatc gagtaaagtc ccacacccaa aaaccccatt ttttttgcga gcagcagttt
      901 atcaagccct aacagaaaag tataaagaaa agtggaaaga acaagaggca atcctaactt
      961 caactcgtgt gtgagaacct gtttgtccca agaaaggcga aaacgatacg gatttcttcg
     1021 atttataaac acaatcaaac aaacagtgaa tagtgctgta cccgcgtaaa ggccatatat
     1081 cccccagggc ctcaccacga ggaccgtgat cagaagacct cctgccgcat taacaatgat
     1141 ctgctcgctc aagatccgga actttttctc agaccttaac agtgttagta aaaaatcata
     1201 aaatcgctgg acaaacaaga agcccgagag aaacaaaagc cctgcaagaa atgctttttc
     1261 gagcttgcgg tattgaattg cgacgaatcc cagcaacaaa accgcgggaa ccatagacat
     1321 cacaagcgaa aaggttaagg tgctgttctt gatgcgatcc gcctgcttaa agtttttttg
     1381 cccacgtaaa acaggatagt cacgagctag cgcacgagta gttccaaggc ttgcgtagcc
     1441 acaatatccc aaaataactt gaataacact ccaaactccc atccccgcag gccccataaa
     1501 gttacgcaac aagaaactat taagaatgcc aattccttgc gccacataat tcgctgcggc
     1561 aaacaaaacc gtatctttaa gcattagaac ttttcgcgtg ttcatgtctt aatttttcga
     1621 gattgataac ctcgaattaa tcttcttggc tggattaccg gcaaccacat caccttcgtc
     1681 aaatgatttc gtaacaacgg agccagcacc aaccaccata ttatttttaa tcacaacccc
     1741 ctttaaaata atcgtcccat accccgtcca agcccctttt tcaattacaa ccataccgcc
     1801 gtttccctcc ccgggcataa gaatctctcc cgtctgggga tcccgcttgt gatcaccgga
     1861 gatgatcgcg gttttgggtc ccaaaattgc cctttcgatc cgaatctttc cagtcaaatc
     1921 gcagtttcga gagatccacg cgcccgacga gatttccata aaaccattca aaaaagaccc
     1981 ctctccaatt gttacatggt caccaataat gatttttttt gcaaaaatac gaacattatc
     2041 acgaatttcg acattattac caattcgcaa ctcacccgac acagcgctaa gcataacgtt
     2101 tctcccgata aaacaattgc gaccgatagt aactttcttg cccccttgga ctttcatgcc
     2161 gtttaaaatc cggcaaccag gtcctatcta ccggaaaata aaacgcgcgc agaaaacccg
     2221 gaaaaggctc gctgaatacg agacggtttt gcaaagcaga atgggcaatg gctccttcag
     2281 gtgttcattc atgtcgattc tccctagcga ggcatgtctt ttggaagata tcggcaaatg
     2341 atttatcgtc tagctgcagt gagggacgga aagtgtcggc attggagtcc aaaaaagctg
     2401 tgacggctat ttcaacgtca taaaaatcgg agaatggttt aagtgaatcc ttgaatattt
     2461 ctccggcctc aggaatgatc cttagatagt ccgagtaaag acaaagcacg gatttcccag
     2521 ccgaggccgc ttcaaagagc gcaccggaag aaccgtccag gatcacgcgg tcggccatct
     2581 ttagagattc aagaaaagag ccggagaaaa tcgatatgtt cggcgcgttt ttctctttga
     2641 gatagacaag agtcgagtcc ttagcccatt gctggttcgg ggagttagca tgtttataga
     2701 taaaatgata gtctggccgg gatgcaaaaa tatcgatcat ttttttctga aagctgaagt
     2761 accaggttaa cggatagttc atgttgttga agcaacggat cctgtcggaa aatttttttt
     2821 ccacataaac gacagttttt aatcccaaag cggaacagcc tcgcccgttc aggcgtcgga
     2881 cacaatccaa gtaatgcgtt gaacgagcca cagcgcaatc tgtccgatac cgctcccgga
     2941 gtgaccggac caaaaaacac tccgacactt catcccgggc gaatgtttgg tggtaggttg
     3001 ccgtatccat gaccccgaaa atctcgttgt ccgccacgaa gctcgcgtgc tcaatgcaaa
     3061 cgcttttcgc gtcgctcatg tatttcgcgc caagcagcgt acccagagaa gccttatccg
     3121 tgtttccccg cgcgaaaaca tatccgatcc catgagtgga aagaaaacgg ccgattttga
     3181 tagcttgcac aagaacttcg ggagcgtcaa tttcaacgaa gcctttcaga aaggggcgga
     3241 taacggacgt gacatcaagg tcgctttgca ctaggaacca gcgaattagc tcattgttct
     3301 caccaagtgc gcccgcggca agcatgcagg cgtttttgat tgcgtgtgtc tcttctcgat
     3361 ttaactccgg ggcaggaata cgcggccgga acaactggtt ttcattgatg ataccgtcag
     3421 tttcctggac taaaatgttc atgcccctgc caaaaagttc cttgagcggg atatcgatgt
     3481 cagggcttcc aacatgcagg aagatagctt tatgagcggc aagctccttt cgtcggacat
     3541 atttgcggta tttaaaaaaa caaagaaggt ctttcgcgag gtgtttaaag aatgactgtt
     3601 taaccggtag cagggtggtt tctgttttgg gttccgcgga aaaacattga ggacggaacg
     3661 acaacccgtg tttgttgcaa gcaagagcta aaatctgctg aaagacccag cggttccggt
     3721 ttttgaattt gaacaccgaa tagctctcgc ccgcagtcag atcatttccg aggtagacga
     3781 tctcacccac gccttctttt tttagccggg gcaacacctg atcgagaatg accgcattga
     3841 tcaggaccga atcaacacag tatttcagcc tgagatagct ggcggtggcc agtggtatct
     3901 tgtattcgcg gcaaagcgga atcccctcct gaaggaacaa gtcaaagcgg gtaaaccatt
     3961 ttagctggtc tttgaaaaat tccggttcgc ggttccgaag gtctttctcg ctgtaaagat
     4021 cctcaagaac gcggtagttg catcccttgg cgtcaagttg tcgggaaaca cgcggatcca
     4081 gagacaggat cgagcttccc tggatcagat acgacggact gtatcgatcg agaaaaacag
     4141 ccgtggctaa ggacatttat gatcccagaa taacccaagg ggtcaaaaca cccggatatg
     4201 acggggtacc gtatactccc gcgatttgtc cggccccgat aataaaggcg ctaaacatga
     4261 ttcagtatcc tgtatttcat ctcagcgatc ttccagtcct cttcggtgtc aatatcctgc
     4321 atttcgcttt cagggataat taatggatat gagttgtcgc cgatcagttt tcccgtagcc
     4381 aagagcaact ccgttcgcac gcaataaaac tgccgtacat catggtaata aggttccaga
     4441 tcctgtgacc ggacattgta attttccggg tgtaacatta ggacttttcc atcgcggatc
     4501 accaggcacc gttggggcgg atatgagaac ttcaccactg ggaaaacgct atcgcatcgc
     4561 ttttcggtca aaagcgccat ggcctgacga agacactctt ctttaataaa cggggcgcaa
     4621 ggatagatgc aacatacatg gtcgaaccgc tttcccgatt ttttatattc ttttataact
     4681 tcaacaagaa caggcgccgt catcgcccga tcattcgaca attcagcact tcgaaagaat
     4741 ggaacttttg cgccacactt ctctgagatt ccggcgattt cagcatcatc cgtcgaaacc
     4801 atcacctcat caaacacatg acttgccaga gcggaagcaa ttgaataggc gatgatcggc
     4861 tggcccagga aatccttgat gttctttcta gggattcttt tcgaacctcc ccttgccggg
     4921 ataatagcga tactcctcat gcgatgtcct cgttttttag cggttcgcca cgcatcaaat
     4981 cttttcgcgc taccttccca atcacttcct cgtagtgctt tggatgcatc cccactccag
     5041 gacggatcga cctaatgttc gccgccgtga aacggtcacc ccttttgata tcttcgacaa
     5101 caaaaagggg gcgagcaaac tttctattcc gctcattaac ggaataatcg atttttccaa
     5161 gtaacttttc cgtattgcgg accgcgtcaa ccatccgctt aaactcttcc ggatttaaag
     5221 aaaacgaagc atcagctccg ccaagcgatc gatcaagagt aaaatgcttt tcaataacgg
     5281 tggctcccaa agcaactccc gcgacaacag tttcgatatt catcgtatga tccgacaagc
     5341 cgatcttgac gccctggggc ctgaactttc tcttcatatc ttcaacggtc aaaagattca
     5401 tgtcctctaa tttcgcagga taagcagacg tgcatttaag gatcgtaata tcatgattcc
     5461 cgacggactt gcagatatcg acgacttcct gaatctcatc aaattcggag acgccgactg
     5521 aaatgattat cggttttccc ttagatgccg cgtatttcac aagcgggtaa tcaaccgctt
     5581 caaaactcgc gatcttgtat cgggcaacac ccaagctttc caaaaaatct accgcagatc
     5641 ggtcaaacgg ggtcgagaaa aactcgatcc cgacagaatc agcgtattcc ttcagctctt
     5701 tttgccattc ccaaggagtg tgcgcctcgc ggtacaggcc atagaaatac ttgccatccc
     5761 aaagcgtccc cccggaaatc ttaaagatct cgtccttgca atcaatcgtc atcgcatcag
     5821 cagtataagt ttgaagctta actgcatccg caccgcaatg ctttgcctca gaaatgattt
     5881 ttctggcaag atcgatcctg ccgcaatggt tcgctgacat ttccgcaatg ataaatgttt
     5941 tacgttgcat ccttttctat ccttagtaga tacttccgat tcttgtagat aaaaaaagcc
     6001 ggatatttat cattatccac aattctcaaa agattaaatt gatgcgcaag cgtcttgcga
     6061 atgtccaatt cgctgtcaat tggtattctt tttctatagt aagtactttc cccaacttgt
     6121 cgccgagcgg tcaacccatc acgcttttga ataaacaaga gacacatttc aacaatttta
     6181 aggccgagct tctcctgcca ttcctttatc aattcatctc ctgttaaacg gagttcatct
     6241 tggaaataga tatctccagc atctattcgt tctgtagctt caaaaagtgt aagcggtata
     6301 cggttctttc cctccaagat ttgccaacta actggcgaca ttccttttcc ataaggcaaa
     6361 gcacttccat gaacaacgat attctgcttg ttcagcttta ataaatc
//
LOCUS       DAOSIO010000090         6362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_11067_length_6362_cov_3.346758, whole genome shotgun sequence.
ACCESSION   DAOSIO010000090 DAOSIO010000000
VERSION     DAOSIO010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6362
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11067_length_6362_cov_3.346758"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2810)
                     /locus_tag="PK590_04425"
     CDS             complement(<1..2810)
                     /locus_tag="PK590_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23876.1"
                     /translation="MKLNNLFIKTVAVFMSIGLMVPSPGYAARQLSTENSPAVLNNLR
                     AELRGMTMDQATGIVTNYLAGLISEKDPDLLIALEIREMQALLRSALVWLEGRAGNFH
                     QNDLSAMPLEEVNKIIGRYFAGEEVSGEELEAAVVAIPDLESRIERLVARSETRTMMR
                     DESILSWKSKHGKSLKQILDTFVKEYDFRGFDGADDVNFPQQVDEELLRWVGNALGSV
                     EFVSRRHGRSVQLKANDSFVIAGDNGPSTQKFKDALIEGLRDAGIHVIDLGVTVSGQL
                     YKSISNLGVQGGLYVTRSHVEVGTNGAKPNINGITLYGEMLQQAKAQILNGLYAKAEQ
                     PGTLDESQAIRDRSRQMYFEALIKSYGQLAELLKTADMKVAFNLNSGSATGYADLFQT
                     LFGKDVTLLKSEGDSWARKGLADPTRKDVKALAHPEANMLQYSKDHPDLFVLNFDLDV
                     DRVSLLQGGELYLGDEMFYAVIEYLLTLDPYKELLKNIYPDSRMKIEFGQLVRHFGGT
                     SKRHPKGHSKVKATIDLLFEKLVREGGYSGKTEFLQAHPGFRIVQSEYSLHFFLTSDK
                     GEAFDDALEFSLFWLTVFAKIKIKHKHPDWSYADYIKDLKKQGIIAESIQIKEQRTPM
                     PDEVKGKVMDRMKDAVLAYFGDRPDFMFVPDWEKDYEGDMKPYTLINIEGVYHLVTPL
                     GEIFWGQSNTSPKVAFGTQSTSAVNTRKLAGVVAALMILTRQRVAPQAPGFQPLEMAD
                     LFKAWLPETHKLSYEEWSVNHPNADEKQYDAWLGEGLEENILANYPSEQAALSEFVPR
                     AELRYELPADTSFATTEVAGPIDFSKVIADGSLVPKNVAQVRATFTNKGDFQEPLIIT
                     SDFAFNSEIPGLPMVPSLAGKNPVVVIVRDANEEQMVADFNAQLPDKVQPVLVAGSVE
                     QAVSMLQDVMADLIRRNLMVADV"
     gene            complement(2874..4394)
                     /locus_tag="PK590_04430"
     CDS             complement(2874..4394)
                     /locus_tag="PK590_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23877.1"
                     /translation="MGCLSPIFASEVFIPSQLGQVRDKVEGSRGRTVVIIEEAHVAYH
                     AQKVLAEILEDLIRHESFRHVFVEGGWGDVSLTGLRHLASGDKRREVGERYLREGKIS
                     GEEYLSLTTDLEMNLWGVEDPGLYATNMKAFLQLSPEQPRLEEEISRIKSRLVRLGSD
                     ILTETQTRILSMRREYESKKGSLLDYVKFLMGHLSDVSGQYPNLTSLLGFAGGSDVYD
                     AEKVSLEKQSAVRFLSRWLTKPELEALLLLDGQGSMEQEIVFLDELLGKINEYSMSPG
                     VPNVDNLENYRNAIKEASRMDTGKLFEELEMAAEDVFMAQMPTVQQQTFFKIFQGVST
                     LEKLISLRLTEDEFLPLAKEKEPFSLREWNEFLSGLTNGGIFFLSPEDGFRFLEPWIP
                     VAVEFYQSARAREQAMVENLVREMDVRSLDRAILVAGGFHSRNFLKALKEQDFTVLLV
                     SPKFTPGDEGAMHEKYLKILKDKWIGATKLGARSSDFETDPGNRRANVLNSEGSIF"
     gene            complement(4771..5307)
                     /locus_tag="PK590_04435"
     CDS             complement(4771..5307)
                     /locus_tag="PK590_04435"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOG23878.1"
                     /translation="MRETLKEKRSMKETRGLVLVFTGEGKGKTTAALGLAVRMIKHGK
                     RVAMVQFFKPNGRHIFPKANFKVWSFGGGFTWNTSRAVNQKSVDQAWRKCLELLKDSK
                     YQLIILDEIHIAIRHKFLRGANVIKALRERASATHVVLTGRGAPATLIRYADLATEMK
                     CLKHPFSKGFPAWRGLDF"
     gene            complement(5309..6085)
                     /locus_tag="PK590_04440"
     CDS             complement(5309..6085)
                     /locus_tag="PK590_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG23879.1"
                     /translation="MTNTMIQVEKLKVKLNGMAILNEVSFSLGRGEILGLVGPNGAGK
                     TTILKVITGLLRPEEGSVNILGKPSRYFSSRSLARKLGYLSQESEVHWPLRVERVVAL
                     GRTPYLMPWEDLRETDMKIIDSALRETDTYHLKGRRVDHLAGGEKALVMMARLLAGEP
                     EMILADEPVQGLDPAHSLQVMELLRRIAESGKGVLVVLHDLALASRFCHRILLLRGGT
                     IVASGRPPEVFSPEHLKTSYHIEAKYGNSDEFYVVPWRRI"
     gene            complement(6135..>6362)
                     /locus_tag="PK590_04445"
     CDS             complement(6135..>6362)
                     /locus_tag="PK590_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015005947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOG23880.1"
                     /translation="FVGLIVPHLLRPYVGYRAGKLLWASALGGAILLLGSDIAVRIIP
                     SLVELKLGVVTSLVGAPFFLFLLFKMRKKIL"
BASE COUNT         1753 a         1738 c         1292 g         1579 t
ORIGIN      
        1 acatccgcga ccataagatt gcgtcgaatc agatctgcca tcacatcctg caacattgaa
       61 accgcctgct caacgcttcc tgccaccaaa acaggctgaa ctttatccgg caactgcgca
      121 ttgaaatcgg cgaccatttg ctcctcattt gcatcccgca caataacaac cacgggattt
      181 ttacccgcaa gcgaaggaac catcggaagc cctggaatct ccgaattgaa ggcaaaatcc
      241 gaggtgatga ttaaaggttc ctgaaaatca cctttattgg taaaagttgc ccgaacttga
      301 gcaacatttt taggcacaag agatccgtct gcaataactt ttgagaaatc aataggccct
      361 gcaacctccg tcgtggcaaa ggaagtatcc gccggaagct catatcgcaa ttctgcccgg
      421 ggaacaaact cggaaagagc cgcctgctcg ctgggatagt tggcaagaat attctcctct
      481 aagccttcac ccagccaggc atcgtattgt ttttcatctg catttggatg attcacactc
      541 cattcctcat aagataattt atgcgtctca ggaagccacg ccttgaacag atctgccatt
      601 tccaaaggtt ggaaaccagg tgcctggggc gcaacccgct gtcttgtcaa gatcatcagc
      661 gccgccacca cccccgccaa cttccgggta ttcaccgcgg acgtactttg cgtaccaaaa
      721 gccactttgg gagaggtgtt tgattgcccc caaaaaatct cacccaacgg agtcactaaa
      781 tgatagacac cctctatgtt gatcaaggta tagggcttca tatccccttc ataatctttt
      841 tcccaatccg gcacaaacat aaaatcaggc cgatcaccaa aataggcgag tactgcatct
      901 ttcattcgat ccatcacttt gcccttgact tcatccggca taggcgtgcg ctgttccttg
      961 atctggatag actcggcgat gattccctgc tttttaaggt ccttgatata atccgcataa
     1021 gaccagtccg gatgcttatg tttgatttta atttttgcaa aaacagtcag ccagaacaga
     1081 gaaaactcca gcgcatcgtc aaaagcctcg cctttgtcac ttgtcaaaaa gaaatgcagg
     1141 gagtactcag actgaacaat ccggaaacca ggatgcgcct gtaggaattc ggttttcccc
     1201 gagtaacctc cttctctcac caacttttca aataataaat cgatggttgc cttgaccttt
     1261 gaatggcctt taggatgccg cttcgaagtg ccgccaaaat ggcgaaccaa ttgcccaaat
     1321 tcaattttca tgcgcgaatc cggataaata tttttaagca actctttata ggggtcgagc
     1381 gtcagaagat attcaatcac agcataaaac atctcatctc cgagatagag ctcccctccc
     1441 tggagaaggc tgacccgatc cacatccagg tcaaaattca ggacaaaaag gtccgggtga
     1501 tctttggagt actgcaacat gttggcctca ggatgggcta acgcctttac atctttacgg
     1561 gttggatcgg ccaatccctt gcgcgcccaa gaatcccctt cacttttaag aagagtgaca
     1621 tccttaccaa acagcgtctg aaataaatcg gcatatccgg ttgccgaacc agaattaaga
     1681 ttgaaagcga ccttcatatc tgctgttttc agaagttctg ccaactggcc ataactttta
     1741 attaacgcct caaaatacat ttggcgggat cgatcacgaa tcgcctgaga ttcgtccagt
     1801 gttcctggct gttccgcctt ggcatagagc ccgttaagga tctgcgcctt ggcctgctgt
     1861 agcatttctc cgtaaagagt gatgccattg atattgggct tagcgccgtt ggtgccgact
     1921 tccacatggc tccttgtcac gtaaagcccg ccctgcacgc cgagattact gatgctctta
     1981 taaagctgcc cagacacagt cacaccaaga tcaatcacat gaatccctgc atctcgcaaa
     2041 ccttcgatta aagcatcttt gaatttttgc gtcgaaggcc cattgtctcc cgcaatcaca
     2101 aaactatcgt tggcttttaa ttgtacggaa cggccatgac ggcgcgagac aaactcaaca
     2161 ctcccgagag cattgccaac ccaacgcagt aattcttcat ccacctgctg aggaaagttc
     2221 acatcatcag caccgtcaaa tccgcgaaaa tcatactctt tgacgaaggt atcaaggatc
     2281 tgtttgagcg atttaccgtg cttcgatttc cagcttaaaa tactctcgtc ccgcatcatc
     2341 gtcctcgttt ccgaccgcgc aaccaaacgc tcgattctgc tctcaagatc tggaatggca
     2401 acaactgccg cctccaactc ttctccagaa acttcttcgc ccgcaaaata tcgccctata
     2461 attttgttta cttcttccag gggcatagcc gaaaggtcat tctgatggaa attaccagcc
     2521 ctcccctcaa gccaaacaag agccgaccgg agcagcgcct gcatctcccg gatctcaagc
     2581 gcaattaata agtcgggatc tttttctgag atcaatcccg ccaagtaatt cgttacaatc
     2641 ccagtagcct ggtccatagt catccctcgc aactcagcac ggaggttatt gagcaccgcc
     2701 ggtgaatttt cggtgctaag ctggcgggca gcgtacccag gactcggaac catcaaccct
     2761 atcgacataa aaacagcaac cgttttgata aataaattat tgagtttcat ttttaaaacc
     2821 cccttagttt ttatttgttt cctcaacccc ggcatcgggt tccagaacca cttttaaaaa
     2881 attgaacctt cgctgttcaa aacattcgcg cgcctattac caggatctgt ttcgaaatcg
     2941 gagcttcgag ccccaagctt agttgcgcct atccatttat ctttcaagat ttttagatat
     3001 ttttcatgca tcgcaccttc atctccaggc gtaaatttgg gggacacaag caggaccgta
     3061 aaatcttgtt ccttgagcgc tttcaaaaaa ttgcgcgaat gaaacccccc ggcaacaaga
     3121 atggcgcggt ccagagagcg cacatccatt tcacgaacca gattttccac catcgcttgt
     3181 tcgcgagcgc gagccgattg ataaaattca accgcaacag gaatccatgg ttccaaaaaa
     3241 cgaaacccgt cttcaggaga taaaaaaaag attcctccgt tcgtgagacc gctcaaaaac
     3301 tcgttccatt cacgcaacga aaacggctct ttttcttttg ctaggggcaa aaactcatct
     3361 tcggtcagtc tcaaactaat caatttttcg agagtcgaaa cgccctgaaa gattttgaaa
     3421 aaggtttgtt gctgaacagt cggcatctga gccataaaaa catcttcagc cgccatctca
     3481 agctcctcga aaagcttacc ggtatccatg cgggaagctt ccttaattgc gttacgataa
     3541 ttttcaaggt tatcaacatt cggcactccc ggggacatgg aatactcatt tattttcccg
     3601 agtagttcat ccaaaaagac gatctcctgc tccatgcttc cttgcccatc gagaagaaga
     3661 agcgcctcca actccggctt ggtcagccac ctggacagga aacgaacagc agattgtttt
     3721 tccaaagata ccttctcggc gtcataaaca tcgctaccgc cagcaaaacc caagaggctt
     3781 gtcagattgg gatattgacc agacacgtcg gacaaatgcc ccatgagaaa cttcacatag
     3841 tccaaaagtg aacccttttt tgattcatac tcgcgcctca tggataaaat tcgggtttgg
     3901 gtttccgtca aaatatctga tccaagccga acaagtctac ttttaattcg tgagatttct
     3961 tcctcaagac gtggctgttc aggagaaagt tgcaaaaaag ccttcatgtt tgtcgcataa
     4021 agtccgggat cttcaacgcc ccaaaggttc atctcaagat ccgtcgtaag actcagatac
     4081 tcttctccgg aaattttacc ttctctcaaa taccgttcac ctacttcgcg acgtttgtca
     4141 ccagaagcca agtgacggag gcctgtcaaa gaaacatcgc cccaacctcc ctcgacaaaa
     4201 acatgtcgga aagactcgtg gcggatcaga tcttcgagaa tttcagccag cactttttgt
     4261 gcatggtaag ccacgtgggc ttcttcgatg atcaccacgg ttctaccgcg agaaccctcc
     4321 accttatcgc gaacttggcc caactgagag gggataaaca cttcggaggc aaaaatcggg
     4381 gagagacatc ccattgggag aagaaaaatt aaaaatagct ttgcgactac atgttgtttt
     4441 ggaaaaaacc gtcgagtcca tttttgaaaa aacttcacaa cccacatggc ttaagcacag
     4501 tgcctttctc gaaataaatt tcaaaacatt taaaaaaatt taacatatgg gcttgaaaac
     4561 tacaagccaa ggaactaaaa acagaaattc cccaacttct gattagtctg ggaaatgttt
     4621 gactaaaaac gggaaaaatc tcttttgggt ggagtcgttt ttaaaaaaat aacaaaatca
     4681 ttccacgcgc aagaatgtta taaattgaca gaactgccgt tcaatcacgg gcccaataaa
     4741 tagccgtagt atcggggaaa tattgaggtt ttagaaatca aggcctcgcc aggcgggaaa
     4801 ccccttactg aacgggtgtt tgagacactt catttctgtc gcgagatcgg cgtatcggat
     4861 cagggtagcg ggagctcccc ggcccgtcaa aaccacatga gtcgcggaag ctctctcccg
     4921 aagggccttg atcacgtttg cgccccgaag aaatttgtgc cggatggcaa tgtggatctc
     4981 gtccagaata atcagctgat attttgaatc tttcagcagt tcaagacatt tcctccacgc
     5041 ctgatcgact gatttttgat ttacagcgcg ggacgtattc caggtaaatc ctccaccgaa
     5101 actccagacc ttgaaattgg ctttggggaa aatatgccgg ccattcggct taaaaaactg
     5161 aaccatcgcg acacgtttcc cgtgtttaat catccgaacc gccaaaccaa gggcagccgt
     5221 cgtttttccc ttcccctctc ccgtaaagac aaggacaaga ccccgcgtct ctttcatgct
     5281 ccgcttttct tttaacgtct cgcgcatttc aaattctcct ccagggaaca acataaaact
     5341 cgtcactgtt accatatttt gcctcgatat gataactggt ttttagatgc tcgggcgaaa
     5401 agacctcagg gggccgtccg gacgcgacaa tcgtcccacc acgcaaaagc aaaatcctgt
     5461 gacaaaatcg tgacgcgagg gccaggtcat gaagcaccac caatactcct ttcccgcttt
     5521 cggcaatacg ccttagcaac tccatcactt gcagtgaatg agccggatcc aatccctgaa
     5581 ccggttcatc cgcgagaatc atctccggct ctccggccaa aagacgagcc atcatcacaa
     5641 gcgctttttc ccctcccgcc aagtgatcca cccggcggcc cttcagatgg tatgtatcgg
     5701 tttctcgcaa agccgaatca atgatcttca tatccgtctc ccgcaaatcc tcccacggca
     5761 tcaaataggg cgttctcccg agcgcgacaa cccgttccac ccgcagaggc cagtgaactt
     5821 ctgattcctg agagagatat cccagtttcc gggccaggga tcttgaagaa aaataacgac
     5881 ttggcttgcc gaggatatta acgctccctt cctccggcct caaaaggccg gtaatcacct
     5941 tgagaatcgt tgtcttcccc gcgccgtttg gcccgaccag ccccagaatc tcacctcttc
     6001 cgagcgagaa agaaacctca ttcaagatcg ccattccgtt caatttaacc tttaattttt
     6061 ctacctggat catcgtgttt gtcatctaaa gccgctactt taaaaatctc aagagaccgt
     6121 tttctgtatt tttgttacag aatttttttc ctcatcttga agagcagaaa cagaaaaaaa
     6181 ggagcgccaa ccagcgacgt cacaaccccc agtttcaact cgacaagcga ggggattatc
     6241 cggaccgcga tatccgaacc aagcaaaagt attgccccgc cgagggcgct cgcccataaa
     6301 agcttccccg cgcgatatcc gacataaggg cgtaaaagat ggggcacaat cagcccgaca
     6361 aa
//
LOCUS       DAOSIO010000091         6360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_11072_length_6360_cov_4.185091, whole genome shotgun sequence.
ACCESSION   DAOSIO010000091 DAOSIO010000000
VERSION     DAOSIO010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6360
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11072_length_6360_cov_4.185091"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..631)
                     /locus_tag="PK590_04450"
     CDS             complement(134..631)
                     /locus_tag="PK590_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOG23881.1"
                     /translation="MIGGGLTKMFFRMQWRILFLCLGVMLAWSPVSFAELITDISAEG
                     VLTLEDGKRVSLAGVVLDAYGVSILRVLAHQQNANVDVLREASGADGCERAYVYLKSR
                     SLDFPFAVGRSADERDMMLNEFLLRLGAARVDESQTFSQKKKFLLIQEEASRKGEGIW
                     SYVQS"
     gene            complement(663..2030)
                     /locus_tag="PK590_04455"
     CDS             complement(663..2030)
                     /locus_tag="PK590_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOG23882.1"
                     /translation="MNEIMDLASHKGGLALRIATALRDHGHTVFFVGGCVRDHLMGKQ
                     PEDIDIATTAKPEEVEAIFPKTIPVGKQFGVVIVVEDDIPFEVATFRAEGGYQDGRHP
                     TRVEFTVPEDDARRRDFTVNGLFYDPFTQKVIDYVAGKGDVDAKLIRAIGDPDERFRE
                     DKLRLLRAIRFASTLGFEIERKTWDSIRTLAASIHAVSPERIRDELVKIFTRPGAARG
                     FMLLHESGLMKEILPEVEAMKGVEQPEEFHPEGDVFEHTRILLTHLTPPVSEVLAFSA
                     LFHDVGKPRTRAIRKGRLTFYEHSEEGARIAREIMRRLRFSNDTIEGVYQSVLNHMKF
                     MDVKKMRSGKLKMFVARPTFLDEMQLHRADCLASHGMLDNYEFLQEKMKEFEHEELKP
                     KALVSGHDLIALGMKPGPEMKPLLGELYELQLEGAIKGREEALTWAKKKIVSLKSQAD
                     GSRAT"
     gene            complement(2049..2369)
                     /locus_tag="PK590_04460"
     CDS             complement(2049..2369)
                     /locus_tag="PK590_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23883.1"
                     /translation="MSHHYLRIFKSYPIEEVKKGLMIAGDLTANCAACNALGIDPYSA
                     ATCPECGVTFRFIASRRLESHPSERFKFARRIADKRPDLTLIDLSDYKSATGDKSAKD
                     FFGF"
     gene            complement(2375..2896)
                     /locus_tag="PK590_04465"
     CDS             complement(2375..2896)
                     /locus_tag="PK590_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23884.1"
                     /translation="MRFLKKIAQSLFGSPGPKKRKTSRVKPRKVRKPKKTVQRASKGK
                     SAVRKKKATKSKATSKNKKTARAPKTSRKPARKKVRAKTPREIKEALAVLPIGEVTHY
                     FPRVNAAVIKINQGQIRLGDVLSLRGHTTRLKQTVDSMQIDHKPILIANPGDEIGIQV
                     KSRVRAGDKVYKV"
     gene            complement(3008..4090)
                     /locus_tag="PK590_04470"
     CDS             complement(3008..4090)
                     /locus_tag="PK590_04470"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOG23885.1"
                     /translation="MEISQILIAILVAVVVILPTVIWILRKSLPQSPQDKTFQSFLQL
                     QQELHNLHSGLQEQIRSVSGQLAEHMQSNARFLQDSQQNYREVMDNVQHRLGVLQGAT
                     QSMVDIGKDISSLQDILRAPKLRGGMGEFLLAELLRQILPDDHFSLQHRFKNGAQVDA
                     VIRLGEGLVPVDAKFPLENFRRILESDPSTEAESRKAFWRDIKKHVDDISSKYILPEE
                     GTFEFAMMYIPSESIYYETIIRDESQNESISSYAIRRKVIPVSPNSFYAYLQAMVRGL
                     KGMRIERSAQIILENLGQLEVSLGQCLSDFEKIGVHLGHARASYEKTELRFRKLQNKL
                     SAMGDGREASKDSDSRELPIPADETV"
     gene            complement(4227..5822)
                     /locus_tag="PK590_04475"
     CDS             complement(4227..5822)
                     /locus_tag="PK590_04475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOG23886.1"
                     /translation="MNRSILITQDANEKQVAILEDGKLEEFYIERPEAKKLFGNIYAG
                     VVKTVIPGIDAAFVDIGTGKDGFLYVGDAMRSPLDLDAEYDEDAEEKPQLQLVQETPP
                     QGQPTQPQPKKAQQKGRFRDRHHRPRRQKIEEVLKPGQKIIVQVVKEAIGNKGPRLTT
                     LFSIPARYLVMMPGEEKIGVSRRIESRGERERIRKIFRDIEIPKGVGFIVRTNAEGKS
                     EKEYTRDVRYLIRLWKKIHSTIQTKKAPALIHSELGLVERVIRDHTNEETTKIIVDHP
                     DIFKKIKRFIEVYMPGQKLPVEMTRERGSLFERHHIDKEIERTFQKNVYLKSGGYIVI
                     EQTEGLVAIDVNTGKFTGNNRGLEETVYKNNVEAAQEIARQLRLRDVGGIIIVDFIDM
                     ETHEHRRNLFRIFKEAVSKDRAKTHLLQVSELGLVEMTRQRIRPSLESAVYDTCPYCE
                     GKGMVKSTATMAIQVIRDLKRALGESRDRILNAYVHPDVAARLLQQEKKSLRHIEKIT
                     GSKISVFPEPGAHREDVNITFIK"
     gene            complement(6123..>6360)
                     /locus_tag="PK590_04480"
     CDS             complement(6123..>6360)
                     /locus_tag="PK590_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007959543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23887.1"
                     /translation="NSAITETKVPYLGDIPFFGKIFRSKRQTPTSNAQVETLFFVTVT
                     MVDSQGQPVIETVDDQAQNPGSQTVATQPDKATP"
BASE COUNT         1495 a         1780 c         1420 g         1665 t
ORIGIN      
        1 gttttttggg tcagatattt ttcaagttcg acgcgaaaaa tattggggtc cagactttta
       61 agaattaaaa aaccggcgat tccgagcacc agcgcggcca caacagcaaa aacaaggcat
      121 ccgaaaaaga atttcaagat tgaacgtatg accatatccc ctcacccttc ctgctggctt
      181 cttcctggat taaaagaaat ttcttctttt gcgaaaacgt ctggctctca tccactcgcg
      241 cggcgcccag tcgcaacaaa aactcgttga gcatcatatc acgttcatcc gcgcttcgtc
      301 ccacggcaaa cggaaaatca agcgaacggg actttaaata aacatacgcg cgctcacagc
      361 catcagcgcc actcgcctcc cgaagcacat cgacgttcgc gttttgttga tgagcgagca
      421 cgcggagaat cgacacgccg taagcgtcga gaacaactcc cgccagcgaa acacgcttcc
      481 cgtcctcaag cgtcaaaaca ccttcagcgg aaatatccgt gatcaactcc gcgaatgaca
      541 cgggcgacca cgccagcata acccccaaac ataaaaaaag aattcgccac tgcattcgga
      601 aaaacatttt cgtcaatccg ccgccgatca tgatgatccc tttccgcgca tggaatccat
      661 gcctatgtcg cccgcgatcc gtcagcttgg gacttgagag acactatctt ttttttagcc
      721 catgtcaagg cttcctcccg tcctttgata gctccttcaa gttgtaactc atagagttcc
      781 ccaagcaacg gtttcatttc gggccccggt ttcatcccaa gcgcgatcaa atcatgacca
      841 ctgacaagcg ctttaggctt aagctcttcg tgctcaaatt ctttcatttt ttcttgtaaa
      901 aattcgtaat tatcaagcat cccgtggctc gcgagacaat ccgccctgtg gagttgcatc
      961 tcgtctaaaa acgtggggcg cgcgacaaac attttaagct tccccgagcg catttttttg
     1021 acgtccataa acttcatatg attcaaaacg ctttgataaa cgccttcaat agtgtcattt
     1081 gaaaacctga gccggcgcat aatctcccgc gcgatcctcg cgccttcctc cgagtgttca
     1141 taaaaagtca gccgtccttt tcggatggct cgcgtcctgg gctttccgac gtcatggaag
     1201 agggccgaaa aagccaggac ttcagaaacc ggcggcgtca gatgggtcag aagaattctc
     1261 gtatgttcaa aaacatcacc ctcgggatga aattcttcag gttgttccac tcccttcatc
     1321 gcctcaacct cgggcaaaat ttccttcatc aggcccgatt cgtgaagcag catgaacccc
     1381 ctggctgcgc ccggccgggt aaaaatcttt acgagttcgt cgcgaatccg ttccgggctt
     1441 acggcgtgga tgctcgccgc gagcgtcctg atagaatccc aggtttttct ttcaatttcg
     1501 aaaccgagcg ttgacgcgaa acgaatcgcc ctcaacaagc gaagtttatc ttcccgaaac
     1561 ctctcgtccg gatcgccaat cgcgcgaatt aatttcgcgt caacatcgcc ttttccggcg
     1621 acataatcga tcaccttttg agtgaacggg tcgtaaaaaa gcccattcac ggtaaagtcg
     1681 cgtcgtctcg cgtcgtcttc gggcaccgta aactcaaccc gcgtcggatg ccgtccgtcc
     1741 tgatagcctc cttcagcgcg aaaggtcgcg acctcaaaag ggatatcgtc ttccaccaca
     1801 atcaccacgc caaattgctt tcccaccggg attgttttgg gaaagatcgc ttccacttct
     1861 tccggcttcg ccgtcgtcgc gatatcaata tcctccggct gttttcccat gagatgatcc
     1921 cgcacgcatc ctcccacaaa aaacaccgtg tgtccgtgat cacgaagggc cgtggcaatc
     1981 cgtaacgcca gaccgccttt atgagacgca agatccatga tttcgttcat cgcccccgtc
     2041 ttctgatctt aaaacccaaa aaagtctttg gcacttttat cgcctgtcgc gcttttataa
     2101 tcggagagat caatgagtgt caaatccggc cgtttgtccg cgatccgccg ggcgaattta
     2161 aatctttcgg aaggatggct ttccagcctc cggctcgcga taaaccggaa tgtcacccca
     2221 cactcaggac aggttgccgc gctgtaggga tcaataccta gcgcgttaca ggccgcgcaa
     2281 ttggctgtca ggtcaccggc tatcataagg ccttttttaa cttcttcgat cgggtaagat
     2341 ttgaaaatcc gcaaataatg gtggctcata aaaatcagac cttgtaaacc ttgtcacctg
     2401 ccctaacgcg cgatttcacc tggatgccga tttcgtcacc gggattcgcg atcaggatcg
     2461 gcttgtgatc aatttgcatc gaatcgaccg tctgcttcaa ccgagtcgtg tgtccccgta
     2521 gcgagagaac atcacccagc cggatttgac cctggttaat cttgattacc gccgcgttca
     2581 cgcgcgggaa ataatgcgtc acttctccta tcggcaacac agccaaagct tctttgattt
     2641 cccggggcgt ttttgcgcga accttttttc gcgcgggctt tctggacgtt ttgggggccc
     2701 gggctgtttt tttatttttg gaagtcgctt tcgattttgt tgctttcttc tttcggacag
     2761 ctgatttacc tttggacgcg cgctgcacgg ttttctttgg tttccggacc ttacgaggtt
     2821 tgacacggga cgtttttctt tttttaggac cgggcgaacc aaacaacgat tgggcgattt
     2881 tttttagaaa tctcatcgaa tgtgtctcct tataagcctt gtgtcagaac caactattcc
     2941 ttctccccac catctcgatc cggcatgccg gggcaaaaca cttttcctca aaacacgcgt
     3001 gctaacttta gacggtttca tccgccggaa ttggcaattc acgggaatca gaatcctttg
     3061 acgcctcccg tccatccccc atggcgctca atttattctg aagttttcga aaacgtagtt
     3121 ccgtcttttc gtaggatgcc cgggcatggc caagatgaac accaattttc tcaaaatccg
     3181 aaaggcactg gccaagactc acctccaact gcccaagatt ttcaagaatg atctgcgccg
     3241 accgctcgat acgcattcct ttcagacccc tgaccatcgc ttgtagatac gcgtaaaaac
     3301 tgttcgggga aaccggaatg actttacgcc ggatggcgta agacgagatg ctttcgtttt
     3361 gagactcgtc gcggataatc gtttcatagt aaatgctttc agagggaata tacatcatgg
     3421 caaattcgaa ggtgccttct tcaggcagga tatacttgga gctgatgtca tccacatgtt
     3481 ttttgatgtc gcgccaaaat gcctttctgg attccgcttc cgtcgatgga tcagactcga
     3541 gaatccttct aaaattctcc aacggaaatt tcgcgtccac cggcaccaat ccttcaccga
     3601 gccgtatcac cgcgtcgacc tgggctccgt ttttgaaacg gtgctgaagc gagaagtgat
     3661 cgtccggaag aatttgtcgc aaaagttcag ccagcaggaa ttcccccatt ccaccccgga
     3721 gcttgggggc ccgaagaata tcctgcaatg atgaaatgtc tttcccgatg tccaccatgg
     3781 actgcgtcgc gccctgaagg actccaagcc ggtgctgaac gttgtccatc acttcccggt
     3841 aattttgctg gctgtcctgt aaaaaacgcg cgttgctttg catgtgttcg gccaactgcc
     3901 cggaaacgga acgtatctgc tcctgaagac ccgaatgcag attatgcaat tcctgctgga
     3961 gctggagaaa actctgaaaa gtcttgtcct gggggctttg tggcaagctc ttgcgaagaa
     4021 tccagatgac cgtcgggagt atgaccacga ccgccaccaa aatcgctatt aagatctgcg
     4081 aaatttccat gaaggcatta tacccgaaag caactggtag ccctagtctc tttcataatc
     4141 agcaggaaca ccatggcgac caaagaaatc ccttccatcc caccacagcc ccggaccggc
     4201 tcgcatcagc ctccgtcaaa tcttttctat ttgataaagg tgatgttgac gtcttccctg
     4261 tgggcgcccg gttcggggaa aaccgaaatt ttgcttcccg taattttctc gatatgccgc
     4321 aatgattttt tctcctgctg gagcaaccgc gcggccacat caggatgcac ataggcgttc
     4381 aaaatccggt ccctggattc tccaagtgcg cgcttgaggt cacgtatcac ctggatcgcc
     4441 atagtcgccg tcgacttcac catccctttc ccttcacagt aagggcaggt atcataaacc
     4501 gcgctttcaa gcgacggacg aatgcgctgg cgcgtcatct ccacaagacc aagctccgag
     4561 acctgcaaaa gatgcgtttt ggcgcgatcc ttgctaaccg cctctttaaa aatcctgaaa
     4621 agatttctcc gatgctcgtg cgtctccatg tcaatgaaat cgacgatgat gatgcctccg
     4681 acgtcacgaa gccggagctg tcgcgcaatc tcctgggcgg cctcaacgtt attcttgtaa
     4741 accgtctcct cgaggccccg attatttccg gtgaattttc ccgtattcac gtcaatcgcg
     4801 accagacctt cggtctgctc aatgacaatg taaccacccg atttaaggta gacatttttc
     4861 tgaaatgtcc gttcgatttc cttatcaatg tggtgacgtt caaaaaggct tccgcgctcc
     4921 cgggtcatct cgacgggaag tttttgtccg ggcatgtaaa cctcaataaa ccgttttatc
     4981 tttttaaaaa tatcgggatg atccacaatg attttggtcg tttcctcgtt ggtgtgatcg
     5041 cgaatcaccc gctccacgag acccagttcg gagtggatca gcgccggcgc ttttttagtt
     5101 tggattgtcg agtgaatttt tttccagaga cggatcaaat aacggacatc ccgtgtgtat
     5161 tccttttctg acttcccctc ggcgttcgtc cggacgataa aacccactcc tttcgggatt
     5221 tcaatatccc taaaaatctt ccggatccgt tcccgctccc cacgcgattc aatcctgcga
     5281 gaaacgccta tcttttcctc gcccggcatc atgacaagat accgggcggg gatcgaaaaa
     5341 agagtcgtca gacgaggccc cttgtttccg atcgcttcct tgacgacctg cacgataatc
     5401 ttctggccgg gtttcaaaac ctcttcaatt ttctgccgac gtgggcgatg gtgccgatcg
     5461 cgaaaccgcc ccttttgctg agcctttttc ggctgtggct gtgtcggttg cccctggggt
     5521 ggggtttctt gcaccagctg gagttgtggc ttttcttcag catcttcatc gtattccgcg
     5581 tccagatcca aaggtgaacg catggcatct ccgacgtaga ggaatccgtc cttgccggtt
     5641 ccaatgtcga caaaggcggc gtcgataccc gggatcaccg tcttcacgac tcccgcataa
     5701 atattcccga aaagcttttt ggcttcaggc cgctcgatgt agaactcctc gagcttgcca
     5761 tcctcaagaa tcgcgacctg tttttcatta gcgtcctgcg tgataagtat acttcggttc
     5821 ataaatagat cctttcaagc ttgcgttctt tcaagattaa ctttgcgccc ggcacagaac
     5881 tctcatcgca tcaaaatcac gaccacagat cgacccgcga catcaatctt gaaactaccc
     5941 tgtttgttgg agggagaccc cggaagtcac caggaaggaa acatgaagac aatctggagc
     6001 actgaaatcc tgaccagcct tctgtgacta attcatcctt gagaaactgg gtttcttaaa
     6061 taccctgcgt gattttctca atggataaat cagctttttt aaaatcatca gaaaaagatc
     6121 tcctacggcg tcgccttatc cggttgggtc gcgacggttt gagatcctgg attctgggct
     6181 tgatcatcaa ctgtttctat cactggctga ccttgagagt ccaccatggt aactgttacg
     6241 aaaaatagcg tttcaacctg cgcattactt gtcggcgttt gtctttttga ccgaaaaatt
     6301 ttaccaaaaa agggaatatc gccaagatat ggaaccttcg tctcggtaat cgctgaattg
//
LOCUS       DAOSIO010000092         6354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_11084_length_6354_cov_4.558343, whole genome shotgun sequence.
ACCESSION   DAOSIO010000092 DAOSIO010000000
VERSION     DAOSIO010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11084_length_6354_cov_4.558343"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1067)
                     /locus_tag="PK590_04485"
     CDS             complement(75..1067)
                     /locus_tag="PK590_04485"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOG23888.1"
                     /translation="MTNKAFITGITGQDGSYLAELLLSKGYEVHGIVRRVALEDPKHR
                     TWRINHIRDRVKLHSASLESYASVFNVVEKIRPDECYHLAAQSFVSYSFEDEFSTMNT
                     NIDGTHAILSALKERAPKCRFYFAGSSEMFGVAPQSPQNETTPLMPRSSYGISKLAGY
                     HLTRNYREAYGMFACSGILFNHESPRRGFEFVTRKITNAVARIKLGLEKELRLGNLDA
                     KRDWGFAGDYVKAMWLMLQQEKPDDFVAATGETHTIREFLDLAFDHVGLDWKKFVSTD
                     KQLFRPAEIFELKGDYSKARKILNWKPEVSFTELVHMMVDADLERHQAHLSAKV"
     gene            complement(1064..2653)
                     /locus_tag="PK590_04490"
     CDS             complement(1064..2653)
                     /locus_tag="PK590_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOG23889.1"
                     /translation="MNLEVVVLGRSLPLERCLGQIGTPFRTLKMDDPVPQESAAYVVV
                     SDSPEVLALAGRLLSQGLAVIMPESFCGRLDSGPLRFTYPDHLLCFSEERIRKQFDVA
                     GLHPTESVAVRSTAHTVFFFLKLLTQVFEVQGLLFPALGFYPSGKRSLFSCRIDADEW
                     DEAVCDPFFRKLKPYSRGVSVFFSAANYSGSPNTIRDCAAAGAEVLSHGYYHYTYRSA
                     RQNRLNLAKASEFLNGLGIFSRGFAAPHGRWNQGLQKVLEDLGYLYSSDFSYDYDSFP
                     SYPFSRDGFSRVLQIPIHPVCSGVCLEASRSGFTHQILLPYFKDVFLRKYYAGLPVIF
                     YDHPTAWAVENFKLIEKWFDLAKEFSDVWICPMGDFATWWSQRTEVNVRLDQSPHGST
                     VRISCETLPRSSQLFSLNLFRQDASEYAPVGLREGVSNFDVSGASFLKRETGAPDAAI
                     PVFERKWSLRLKATVKEFLDWETTTPSDLLLADDFSSFLKKMARLSFDRVRLRHNDQN
                     CEQKGKTKSLRNKIFIERNQR"
     gene            complement(2653..4080)
                     /locus_tag="PK590_04495"
     CDS             complement(2653..4080)
                     /locus_tag="PK590_04495"
                     /inference="COORDINATES: protein motif:HMM:NF019615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG23890.1"
                     /translation="MELSVVLIVKNEEKHLEECLSALRFADEIVIVDSGSTDRTVDIA
                     KGFTDKVFSNAFSDFSSQKNFAVSQAAGKWILVVDADERVSPELAAEIRAVCSKGEGC
                     VAYRIHRQTYFLDKRMRFSGMQDDRPIRLFKRGSGRFEQPIHEYYRCEGAVGDLKNDL
                     IHHTTPTIDVEISKTELYTDLEARFLIAQGVRPSFWNSMKPLLVFVRLYFFRLGFLDG
                     MPGLQYAYFSARYCFRKYRKFNELYQQAVLETKIKERFDDFAKTLPKKICRSDSRLNA
                     LLAACGSLKDKKVLEVGCGKGRFVEVMLENGAAVTGVDPSDVLLEDARKLKGGTFLQS
                     SASQLPFEDGTFDIVYTVEVVGHLPLLERSIAEMSRVLKKNGCLVIVDRNKFSINHKR
                     FLAPNFLIKRWHELKDHWIYPKGFPFRERWFSKSGLSRLLRRYFANVTASYIESDGEK
                     RCPWHFLFDLIPMARVFVLWKGVKN"
     gene            complement(4082..5044)
                     /locus_tag="PK590_04500"
     CDS             complement(4082..5044)
                     /locus_tag="PK590_04500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG23891.1"
                     /translation="MTRQISEARIVILNYNGEDLLTECLPSIVEAAHCSRRRTVVTVL
                     DNRSTDGSVELLRKRFPTVEVMVAAQNRILFSYNDALARMTEPVVILLNNDIRVDKNF
                     VDPLIDPFERDERVFAVGARCRDFDGKGFQGEKSIAGMRWGLFWTDSRYRGYEKDIER
                     PSWTAQVALGAFDREKFLQLGGYDDLYFPGLWEDTDISFRAYRRGWHCLYEPQSIIYH
                     KGQVSFHREYGSRERAVIAYRNTFLFMWKNLRESSLALSQWFWVPLRILVTLAKGNTG
                     LLIGFFRAFRMRHRVASGTDQNCPRPERTIRQVLSIISSRDWRP"
     gene            complement(5041..5586)
                     /locus_tag="PK590_04505"
     CDS             complement(5041..5586)
                     /locus_tag="PK590_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG23892.1"
                     /translation="MKILDVGCGNKKREGSIGIDSNPKTQADVIHDLNRFPYPFEENQ
                     FEEIYVTHCIEHLDNVIAVMEELHRIGKSGCRVIIEAPYFSGQDAFSDPTHKHFFTSR
                     SFDYFTGDFPEFSFYSSKARFKKLDFHIDFWKVHMLGGVRLQNYLGCGFLANQLTGIY
                     EKFFAFIFPAQSIRYVLEVVK"
     gene            complement(5621..>6354)
                     /locus_tag="PK590_04510"
     CDS             complement(5621..>6354)
                     /locus_tag="PK590_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOG23893.1"
                     /translation="REKIQAPRRSVDTWYGRHVMRFFSIYLTRFFIRWNWTPNQATLL
                     SLVMGLGGACFLGVGLWGIGILGINLWYLVDHVDGEIARYTGKTSVTGYFFDTAVNFF
                     VQPATFFAIGVGLGSPWGGAAGVIAAFGNLMLSMIPMCEDSIRLSICRKNGSLPVSTV
                     PEIQPNAVPGVMKRIFVFLHRVVTYPVFLLILTGSYLFFFVFRVMQMRTLMLVILVLY
                     AGCVSLVWIAQLVHKILTRKLDRSF"
BASE COUNT         1800 a         1726 c         1417 g         1411 t
ORIGIN      
        1 aggcttctat caccggcgcc attttcagcg cctccggccg cgtcccaaaa acaaaaagga
       61 tactcgactg catcttagac tttcgcggaa agatgggctt ggtggcgttc gagatcggcg
      121 tcgaccatca tatggactaa ttccgtaaaa ctgacttccg gcttccagtt taagatcttg
      181 cgcgctttgg aataatcccc cttgagttca aatatctcag cgggacggaa aagctgttta
      241 tccgtgctaa cgaacttctt ccaatcaaga ccgacatgat cgaatgcaag atcaaggaat
      301 tctcggatcg tgtgggtttc tcctgttgcc gcgacaaaat catcgggttt ttcctgttgt
      361 aacatgagcc acatggcttt gacataatct ccggcgaacc cccagtctct tttcgcatcg
      421 agatttccga gtcgaagctc cttttcgagg cccagtttga tacgcgcgac ggcattcgtg
      481 atcttgcgcg tcacgaattc aaaccctcgg cgaggcgatt catgattaaa gaggataccg
      541 ctacaggcaa acattccata agcttcacga taattgcgcg tcaggtgata accggcaagc
      601 tttgagatgc catatgaaga tctgggcatc agcggcgtgg tttcgttctg cggactttgc
      661 ggagcaactc caaacatttc gctggatccc gcaaaataaa agcgacactt tggggcccgt
      721 tcttttagcg ctgaaagaat agcatgggtt ccgtcaatat ttgtgttcat tgtcgaaaac
      781 tcatcttcaa atgaataact gacaaagctc tgagccgcca ggtgataaca ttcatccgga
      841 cgtatttttt caacaacatt aaacacactc gcataacttt ctagcgacgc cgaatgcaat
      901 ttcacgcgat cacgaatgtg attgatcctc caggttcggt gcttgggatc ctcaagagca
      961 acacgacgga ctataccgtg cacttcatac cccttagata aaagtaattc tgccaggtaa
     1021 gaaccatctt gccccgtgat ccctgtgata aaagctttgt tcgtcatctt tgattcctct
     1081 cgatgaatat tttattgcga agactcttgg tttttccctt ttgttcacag ttctgatcgt
     1141 tatggcgtag tctaacacgg tcaaaagaaa gccgtgccat tttttttaaa aaactcgaaa
     1201 aatcatccgc caaaagcaag tccgacggcg tagtagtctc ccagtccagg aactctttca
     1261 ccgtggcctt gagacgcaaa ctccatttcc gttcgaaaac cggtatcgcc gcgtccggag
     1321 cgccagtttc gcgctttaaa aacgacgccc ctgaaacatc aaaattgctt accccttctc
     1381 gaagaccaac tggagcatat tcactggcgt cttgacgaaa caaatttaaa gaaaacaact
     1441 gcgaggaacg cggtaaagtc tcgcatgaaa ttcgtactgt agaaccatgg ggtgattgat
     1501 caagcctaac attaacctct gtccgctgtg accaccaagt cgcaaagtca cccatcgggc
     1561 atatccatac atccgaaaat tccttggcga gatcaaacca cttctcgatc aatttgaagt
     1621 tttccacggc ccaagccgtc ggatgatcat aaaaaattac gggaagacca gcataatact
     1681 ttcgcaaaaa gacatcttta aaataaggta acagaatctg atgagtgaat cccgaacgcg
     1741 aagcctcgag acaaacaccg ctacaaacag gatggattgg gatctgcaat actcgcgaaa
     1801 aaccatcccg cgaaaaggga taggacggaa agctatcata gtcgtaagaa aaatcagaag
     1861 aatacaaata tcccaagtct tcaagcacct tctggagacc ctgattccag cgaccgtgtg
     1921 gggccgcgaa tccccgtgag aagataccca atccatttaa aaactcggat gctttcgcaa
     1981 gattaagacg gttctgccgg gccgagcggt aggtgtaatg ataatacccg tgcgacagaa
     2041 cctcggcccc cgccgccgca caatcccgga tcgtgtttgg ggaacctgaa taattagcgg
     2101 cagaaaaaaa tacggagact cccctgctgt aaggttttaa cttccggaaa aagggatcgc
     2161 aaacagcttc atcccactca tccgcatcaa tacgacaaga gaataaactc cgtttccctg
     2221 acggataaaa gccaagcgcg ggaaacagca acccctgtac ttcaaaaacc tgtgtcaata
     2281 atttcaaaaa gaaaaacact gtatgagcgg tcgaccgaac cgcaacagat tccgtgggat
     2341 gcaaaccggc aacatcaaat tgtttgcgga tccgttcttc cgagaaacac aataggtgat
     2401 cgggataggt aaatcgcaga ggcccgctat cgagacggcc acaaaaagat tccggcatga
     2461 tcaccgccaa tccttgcgac aaaagccggc cggccaaggc gagaacctcg ggcgagtcag
     2521 agacaacaac ataggcggcg ctctcctgag gaacgggatc atccatcttg agcgtccgaa
     2581 agggtgttcc gatctgcccg agacaccgtt ccaacggcaa tgacctcccc aaaacgacca
     2641 cttcaagatt catcagtttt tcacaccttt ccaaagcaca aagactcgtg ccatcggaat
     2701 gagatcgaag aggaaatgcc aagggcagcg tttttctccg tctgattcaa tgtatgacgc
     2761 tgtaacattt gcgaaatatc tccgtagaag acggctgagc cccgattttg aaaaccaccg
     2821 ttcgcgaaac ggaaagccct tgggataaat ccagtgatct ttgagttcat gccagcgctt
     2881 gatcaaaaaa ttgggtgcca ggaaccgctt gtgattgatc gagaacttat tgcggtcaac
     2941 gattacaagg catccattct ttttaaggac gcgggacatc tctgcgatag accgctctag
     3001 gagcggcaaa tgtcctacaa cctccactgt gtacactata tcaaacgtac cgtcctcgaa
     3061 cggaagttgg cttgcgctcg attgcaaaaa agttccaccc ttcagctttc gagcatcctc
     3121 aagcaataca tctgaaggat caacccccgt aaccgccgcg ccattttcaa gcattacttc
     3181 gacaaatctc cctttaccac aacccacttc aaggactttt ttatccttaa gcgacccgca
     3241 ggctgcaagt agagcattca ggcggctatc ggaacgacaa atctttttgg ggagagtttt
     3301 tgcaaaatcg tcaaaccgtt ctttgatctt cgtttcgagc acggcctgct gataaagttc
     3361 gttgaacttc cggtatttac ggaaacagta ccgcgccgaa aaatacgcgt attgaagacc
     3421 cggcatccca tccaaaaaac caagcctgaa gaaatataaa cgcacaaaga ccagcaaggg
     3481 cttcatcgaa ttccagaaac tcggccgcac accctgcgct atcagaaaac gggcctcgag
     3541 atcggtataa agctctgttt tactgatctc cacatcaatc gtgggagtcg tatgatggat
     3601 caagtcgttc ttgaggtcac cgacggctcc ttcgcaacgg tagtattcat ggatcggttg
     3661 ttcaaaccgt ccgctcccgc gtttaaaaag gcggatcggc cgatcatcct gcataccgct
     3721 aaaacgcatt cgtttatcca ggaaatacgt ttgacgatga atgcggtagg caacgcaccc
     3781 ctcccctttg gaacacaccg cacggatctc tgcggcaagt tcaggcgaca cgcgctcatc
     3841 ggcatcaacg acaaggatcc atttccccgc cgcctgagag acggcaaaat tcttctgcga
     3901 gctgaagtcc gagaaggcat tagaaaaaac cttgtccgta aaccctttgg caatgtcaac
     3961 cgtcctgtcc gtgcttcccg aatcaacgat cacgatttca tcggcaaaac gcaaagccga
     4021 aaggcactcc tcaagatgct tctcctcatt tttgacgatc aggacaacgg aaagctccat
     4081 attagggcct ccaatcgcgt gagcttataa tcgataacac ttgccgaatc gttcgttctg
     4141 gccgggggca gttttggtca gtcccggaag ccacacgatg gcgcatccga aacgcccgga
     4201 aaaaaccgat caggaggccg gtgttccctt tcgccagtgt caccaagatg cgtagcggaa
     4261 cccagaacca ttgagacagg gccagcgaac tctcacgaag attcttccac atgaaaagga
     4321 acgtattacg gtaggcgatc accgcacgtt cgcgggaacc gtattcccga tgaaacgaaa
     4381 cctgcccctt atgataaata atgctttggg gctcataaag acagtgccag ccccggcggt
     4441 aggcccggaa tgagatatcg gtgtcttccc agagacccgg aaaataaagg tcgtcatacc
     4501 cgcccagttg aaggaatttt tcccgatcga acgcgccaag agcgacctgc gcggtccagg
     4561 atgggcgctc gatgtctttc tcgtatcccc ggtaacggga atcagtccag aaaaggcccc
     4621 agcgcatgcc agcgatcgat ttttccccct ggaatccctt tccgtcaaaa tcacgacacc
     4681 gcgcgccaac cgcaaaaaca cgttcatccc gttcaaaagg atcaatcaac ggatccacaa
     4741 agttcttgtc aacccggata tcattattca agagaatcac aacgggttcc gtcattcggg
     4801 ccagcgcgtc attgtatgaa aaaaggatcc ggttttgagc cgctaccata acttccaccg
     4861 taggaaaccg ctttcgaagg agctccacgc taccgtccgt actccgattg tccagtaccg
     4921 tcacaactgt ccttcgcctg gagcaatgcg ccgcctccac aatggacgga agacattccg
     4981 taaggaggtc ctccccgtta tagtttagaa tcacgattcg cgcctcgcta atttgacggg
     5041 tcacttcacg acctcaagca cataccggat cgactgcgcg ggaaaaatga aggcaaagaa
     5101 tttctcgtaa ataccggtaa gctggttcgc gaggaatcca caccctaaat aattctgcaa
     5161 cctcacccct ccaagcatgt gaactttcca aaaatcaatg tgaaagtcga gttttttgaa
     5221 acgggccttg ctcgaataaa aggaaaactc gggaaaatcc ccggtaaaat aatcaaagct
     5281 ccgggaggta aagaaatgct tgtgagtcgg atcgctgaac gcgtcctggc cgctgaaata
     5341 cggggcttcg atgataaccc ggcagccgga cttcccgatc cggtgaagct cctccatgac
     5401 cgcaatgaca ttatccagat gctcgataca atgggttaca tagatctctt cgaactgatt
     5461 ctcctcaaac ggatacggaa agcggttcag atcatgaatg acatccgcct gcgttttcgg
     5521 gttcgagtcg atcccgatcg agccctctct ttttttattc ccacatccaa catcaaggat
     5581 cttcataacc tccacccagc acagacgatg agaatttgat ttaaaacgaa cgatcgagtt
     5641 ttcgcgtgag gatcttatgg accaactgcg cgatccagac caaactgacg catcccgcat
     5701 acaatacgag aatgaccagc atgagcgttc gcatttgcat gacacgaaaa acaaaaaaga
     5761 acaaataact gcctgtcaag atcagaagaa acactgggta agtcaccacc cgatggagaa
     5821 aaacaaagat cctcttcatg actcctggga cagcgttcgg ttggatctcg ggcacagtac
     5881 tcaccggtag ggacccgttc tttcgacaga tggacaaccg gatcgaatcc tcgcacatcg
     5941 ggatcatgct cagcatcaag tttccaaacg ccgcaataac accggccgcg cccccccaag
     6001 gagaccctaa tccaacccca atcgcaaaaa aagtcgccgg ttgcacaaaa aagttcacgg
     6061 ccgtgtcaaa aaaataaccg gtgaccgatg tctttccggt ataccgagcg atttccccgt
     6121 cgacgtgatc aacaaggtac caaagattga tcccgagaat gccgatgccc caaagcccca
     6181 ctcccaaaaa gcaggccccg cccaacccca tgacaagcga aagcaacgtc gcttgattcg
     6241 gtgtccaatt ccatcgaata aaaaaccggg taagatagat tgaaaaaaaa cgcatgacat
     6301 gccggccata ccaggtatca acggagcgtc gcggcgcctg tattttttct cgta
//
LOCUS       DAOSIO010000093         6334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_11122_length_6334_cov_3.325530, whole genome shotgun sequence.
ACCESSION   DAOSIO010000093 DAOSIO010000000
VERSION     DAOSIO010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11122_length_6334_cov_3.325530"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>6334
                     /locus_tag="PK590_04515"
     CDS             <1..>6334
                     /locus_tag="PK590_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23894.1"
                     /translation="AQNKDAFIHELLVPNTRLAAGQYEAAAMRLDRVHQERLRLFAYL
                     NAGFGASLEDLGAWTFVVNGGVGFDIPLLSFTRSAAKYSAEAAKERADSHLLDLETRT
                     HFELKVLLDRLVTMKGQIAGQEKRVQTAQELLAKRVVEVRKRLKERKVAVDAETDRIL
                     TALSKGETDELARLVPALVQVSADQRLEEKSWEALAGAANELVKEKTSLSAMIDNRVK
                     VQLLVKRLATPDFRPIPSENALEQADEQFTRDAYQAQLKRVTNPYFRFLDENGGFLES
                     AAKDLTAMEGVLSGFLPEMKKAADSAWAKHPEFKRMKKASDVEKEGLIAEKKLALEER
                     RVALAGKRKQLDAEKPGEKEDKKTQREWRKKLNELQKEQQKLDEELLELGKVESLIRT
                     YKLFQDSQKKLQELVKAANGKSTEDQRHAMRDLLGGLVDGTESYLTSEYLLAKMLDLG
                     AYESRDEWYQGIQWILEFIGDEGTSVFGYTGRIEAIQAAFVNVEKAWAVMKAELDIYV
                     GVGYQRSIVQPETGEATTGSVFTLGFGGVFNHLKRAILGPRSGAQQTAAQLLAESSRA
                     IIERARAHAQESKETWGIQSEMGKLTARIKLYEKIKEEFQQWRSSYEPRPDGVRTHVD
                     RLRSLLVNDRATDEIYSLKTADLDARIQSDTAAIQYLESQLKRLGFSPDAIRQGTAYN
                     EGLQTETTALAPIDEQLREMSRESYSRQSATLNSMLDSVLARSTMGPSAYVTSASMAQ
                     SPFYRDAGVFYQSLDADAGLPGADSNRFAVFSWNGADLFGSQKQIYTLMLRHTRSLED
                     SAVKENERVIQSQVRSVVSAASRLRVLNRMNKLLSDQVSGLQKLEAMIGASRYEHGIK
                     ARSYGALETIALHESEDAFAANLIALWTEVKGMSVGEGEKKKIFHDRLATLRKVEETR
                     LAEDLDSLKTKAGQLGISFDLVEGLDLTKVGNLENYESDEAVRARVREEINEQFAKIL
                     DQNRLQFSVKFGLSHPSRQSELSKGEPFLGFGASYLIYDSNLQAMMNFWENRGSIEQL
                     WAKQGDQDDKARWVELGLDVQKLQKEVELRRDVVEAVATAYRIARERARRDGYPDAPI
                     DAAFRYMNELKAYKGAADAYWEASQRMIDFAREKGIKIELPRGTKDSIDRIKAMIPQW
                     IEDDTTGDVTAPKYLEETVKPELTGQGDRAPGFSIDDESTYPELKAPATELMKVLDAD
                     SANQSQTKKDELAETERESQLLEDQAKEIKRGIEAANRKVEPIDERKAELMEEKKRQE
                     DNKARLEKELGKERQPVSERGILQRIWDWILRLFQKRSVPIEGTAVSGPIALTAVPGA
                     PNGLQTQIQQAEESIKSIDAQVAQLEREMAGILAEIEDLSNELRAIEQQLADLRERVG
                     VLKRKLDRTIMLLTNMLGRVAERYGRKDPAAWVARVTESYQYLQRSGFFEKTGFMEVG
                     LGEEIWGNLFGQCFSMGPEAHKLLEERGIDMNQLAIAAFMGELNQLAVSRGEEGDSQK
                     IAQAIANDFPEAIAALEEMREQPKGTYSLRSPVQGAEVLTLVLYRALGDDELRNLTFD
                     KILNDKDHRRAIENLSPEFNLNNIFPGIMGVERNKDDWVTGWKEATEFLSIASGLYDA
                     KTQTVKRREIPVGDPEEFAYVMAQNIATILAHPRPGRDATPAQIADYQKMMEQSAYLT
                     ARFSHHLANSKNLTIKEEDLTSTTYLRLKALEAKMIAEGKTEVIDPVSGAKVTLEDVK
                     TRKEASRDAEIEGRLMSNALAYVNDILFGSGHDAGENRKEWLAKDLSERVRLTEERVK
                     LEDVFWGEKDLQGYLTELTTRDGKKYKGESPATAMDIYKDKRQVAGSISNMALEVLGE
                     TGKTLDRESLAYMSKLETEIAELQARIENSETSEADKLSAQASLKGKEFTLERFNHAK
                     AKKEADYKVFMGQSGGKDGKTWTKNDRIQSVIEMRRIMSSLRTSVEGLYGSLDTSDPR
                     DSGILRTLAEFVYGDVQSGYMPSALGFDDPEEWEKWQAMSIEERLTVLTDRLQIAGNL
                     NRSLQKAGISLDLKDTHVTNLGEKGEDVGLLMSIAFDLQKDSDAWVEFFDKSGELAGN
                     TDETRQTARTRVNRLFGLILSPAGRAIIQEYLGGPGS"
BASE COUNT         1697 a         1355 c         1870 g         1412 t
ORIGIN      
        1 agcccaaaat aaagacgcat ttatccatga attgctggtt cccaataccc gtctggcggc
       61 cgggcagtat gaagcggcgg cgatgcggct ggaccgggta catcaggaaa ggttgcgtct
      121 ttttgcgtac ctgaacgccg gtttcggagc ttcgctggag gatcttggcg cgtggacgtt
      181 tgttgtgaac ggcggagttg gttttgacat tcctctcctg agttttactc gaagcgccgc
      241 gaagtattca gcggaggcgg caaaggaacg cgccgattcg catttgctgg atcttgagac
      301 ccggacgcat tttgaactta aagttttgct tgaccgcctc gtgaccatga aaggacagat
      361 tgcgggccag gaaaaacgcg ttcaaaccgc tcaggagctc ctcgcgaaac gtgttgtgga
      421 ggttcgtaaa cgtcttaagg agagaaaggt cgcggttgac gctgaaacag accgtatcct
      481 taccgcgctg tcaaagggcg agacggatga gttagcaaga ctcgttccgg ccctggttca
      541 agtgtctgcg gaccagaggc ttgaagaaaa aagctgggaa gctctggcgg gtgcggcgaa
      601 tgaattggtg aaagaaaaaa ccagtctatc cgccatgatt gataaccggg tgaaggttca
      661 gcttcttgtc aaaaggctcg cgacgccgga ttttcggccg attccttcag agaatgctct
      721 ggagcaggct gatgagcaat ttacccgtga tgcctatcag gcacaattaa aacgggtcac
      781 caatccttac ttccggtttt tagatgaaaa tggtgggttt ttagagtcag cggccaaaga
      841 tttgacggca atggagggtg tgctttcagg attcctgccg gaaatgaaga aggcggcgga
      901 ttcagcatgg gcgaagcatc cggaatttaa aaggatgaaa aaggccagcg atgttgagaa
      961 agaggggctg atcgccgaaa aaaaactggc attagaagaa agacgagtgg cgttggctgg
     1021 aaaaagaaag caacttgatg ccgaaaaacc cggcgaaaaa gaagataaaa agacccaacg
     1081 ggagtggcgg aaaaagctta atgaactgca aaaagaacaa cagaagttgg atgaggaatt
     1141 gctggaactt ggcaaagtgg aaagtttgat acggacttat aaactttttc aggattccca
     1201 aaagaaactt caggaattag tcaaagcagc caatgggaag tcgacggagg atcagcgcca
     1261 tgcgatgagg gatcttcttg gcgggcttgt tgacggtacg gaatcttact taacttccga
     1321 atatcttctt gccaagatgc ttgatcttgg agcgtatgaa tcccgtgatg aatggtatca
     1381 gggcatccag tggattctgg aatttatcgg ggacgagggg acctccgtgt tcgggtacac
     1441 gggcaggatc gaggcgatcc aggcggcgtt tgtgaatgtc gaaaaggcct gggcagtcat
     1501 gaaagcggaa ctggatatat atgtgggagt tggatatcag cgatccatag ttcagcctga
     1561 gaccggtgaa gctacaacgg gttctgtttt tacgcttggt ttcggaggtg tttttaatca
     1621 tttgaaacgg gcgattttgg gaccccgatc cggcgctcaa caaaccgcgg cgcaactatt
     1681 agcggaaagt tcccgcgcca ttattgagcg ggcaagagcc catgcccagg agtcgaagga
     1741 aacatgggga atccaaagcg agatgggaaa actgacggcg cggatcaagc tttatgagaa
     1801 gatcaaagag gagttccagc agtggcgttc ctcctatgaa ccaagacccg acggtgtccg
     1861 gacccatgta gatcggttac ggtcactcct tgtgaatgat cgcgctacag atgaaattta
     1921 tagcctgaaa acggctgatc tggacgcgcg tatccaaagc gatacggccg ctattcaata
     1981 tctggagtct cagctaaagc gcctgggttt ttcaccggat gcaattcgac agggaacggc
     2041 gtataacgaa ggcctccaga ctgaaacgac cgcgctggcc ccgattgacg agcagttgcg
     2101 cgagatgagc cgggaaagct atagcaggca gtccgctaca ttgaattcca tgctggattc
     2161 ggtgctcgct cgatcgacca tgggcccttc cgcctatgtg acttcagcat caatggcgca
     2221 gtcgccattt taccgcgacg cgggggtttt ctatcaaagc ctggatgcgg atgccggatt
     2281 gccgggcgcc gacagtaatc gtttcgcggt ttttagctgg aacggcgccg atcttttcgg
     2341 gagtcagaaa caaatctaca ctcttatgtt gcggcatacg aggagtcttg aggattcggc
     2401 ggtcaaagag aacgaaaggg tgattcaatc ccaggttcgg agcgttgttt cggcggccag
     2461 tcggttgcgt gttttgaacc ggatgaacaa gcttctctcg gatcaggtgt ctggacttca
     2521 aaagctcgaa gcaatgattg gcgcgagtcg ttacgaacac ggtatcaagg cgagaagtta
     2581 tggggctttg gaaaccattg cgttacatga atctgaggac gcgtttgccg ccaacttgat
     2641 cgcgctctgg acagaagtca agggaatgag tgtcggcgaa ggggagaaaa agaagatttt
     2701 ccacgaccgc ctcgcgactt tgaggaaagt ggaagaaacc agactggcgg aagatctcga
     2761 cagtctcaaa acgaaggccg gtcagttggg gatttcattt gaccttgttg agggcctgga
     2821 tctcacgaag gttggcaatc ttgaaaatta cgaatcggat gaagcggttc gcgcccgggt
     2881 ccgggaggag atcaacgaac aatttgccaa aatactggat cagaatcgcc ttcagttttc
     2941 tgtgaagttt ggcctgagcc atccgtctcg ccagtcggag ctttcaaaag gcgagccgtt
     3001 cctcggtttt ggagcttctt acctcattta tgattctaat ctccaggcga tgatgaattt
     3061 ttgggaaaac cgaggatcca tcgaacaact ttgggcgaag cagggagatc aagatgacaa
     3121 agctcgctgg gttgagcttg gccttgacgt ccagaagctt caaaaagaag tcgaactgcg
     3181 cagagatgtc gtggaagctg tcgcgacagc gtatcggatc gctcgcgagc gggcgaggcg
     3241 cgatggatat ccggacgccc cgattgacgc cgcgttccgc tacatgaacg aactcaaggc
     3301 ctacaagggg gccgcggatg cctactggga agctagtcag agaatgattg attttgccag
     3361 agagaaggga atcaaaatcg aactgccacg tggcactaag gattcgattg atcgcatcaa
     3421 ggccatgatc ccgcaatgga ttgaagatga tacgaccgga gacgtgactg ctccgaaata
     3481 tcttgaagag accgtgaaac cggaactgac cggccaaggt gaccgagccc cgggtttttc
     3541 aattgatgat gaatccacgt atccagaatt aaaagcacct gccacggagc taatgaaagt
     3601 tctcgatgcg gactctgcga accagtcaca aacaaagaag gatgagttgg cggaaacaga
     3661 gagggaaagt caattgctgg aggatcaggc caaagaaatc aagagaggga ttgaggcggc
     3721 gaaccgaaaa gttgagccga ttgatgagcg caaggcagag ctgatggagg aaaagaaaag
     3781 gcaggaagat aataaggcgc gcttggaaaa ggaattggga aaggaaagac aaccggtgag
     3841 tgagcgcggc atattacaaa gaatttggga ctggatcctg cgattattcc aaaaacggtc
     3901 tgtgccgatc gaaggaacgg ccgtgtctgg gccgattgct cttactgctg ttcctggtgc
     3961 tccgaatggg cttcaaactc aaattcagca agctgaggaa tctatcaaat caattgatgc
     4021 tcaagtagct caattggaac gcgagatggc cgggattttg gcggagatcg aagatctttc
     4081 aaatgagctc cgggccattg aacagcagtt ggcggacctg agggaaagag tcggggttct
     4141 taagcgaaag ttagatcgca cgatcatgct tttgacaaat atgctggggc gtgtggcgga
     4201 acggtatgga cgaaaagatc cggcggcttg ggtggcgcgg gtcaccgagt cttatcaata
     4261 tcttcaacgg agtggctttt ttgaaaagac cggatttatg gaggttggtc ttggtgaaga
     4321 gatttggggt aacttgttcg ggcagtgttt ttcgatgggg cctgaagctc ataagttact
     4381 tgaagagcgc ggtattgata tgaatcagct tgccatagcg gcgttcatgg gtgagttgaa
     4441 tcagctggcg gtttcccgtg gcgaagaggg cgattcccaa aagatagctc aagcgatcgc
     4501 caatgatttc ccggaggcga ttgccgccct tgaggagatg cgagaacagc caaagggaac
     4561 ctattccctg aggagcccgg ttcagggggc ggaagttctg acgcttgttc tctaccgagc
     4621 gttgggggat gatgaattga gaaatttgac attcgacaag atcctgaacg ataaggatca
     4681 ccggcgtgcg atcgaaaatc tttctcctga attcaatctg aacaatattt tcccgggaat
     4741 tatgggtgtt gaacgtaata aggacgattg ggttaccggg tggaaagagg ccacggagtt
     4801 tctctcgatc gcttccggtc tttatgatgc caagacccag acggtcaagc gccgcgagat
     4861 cccggtgggt gatcctgaag aatttgccta tgttatggcc cagaacatcg cgactatcct
     4921 ggcacatccg cgccccggcc gggacgcgac ccccgcgcag attgctgatt accaaaagat
     4981 gatggagcag tcggcctatc tgacggctcg attttcgcat catcttgcga actccaagaa
     5041 cttgaccatt aaggaagaag atttgacgag tacgacctac ctccggttaa aagccctgga
     5101 agcgaagatg atagccgaag gaaagacgga agtgatcgat cctgtgtccg gcgcaaaggt
     5161 gactttagaa gatgtcaaga cccggaaaga ggcgtcaaga gacgcggaaa tcgaggggcg
     5221 tttgatgtcg aacgcgctcg cctatgtaaa tgatatcctt tttggttcag gccatgacgc
     5281 cggagaaaac cgaaaggaat ggcttgcaaa agatctttcg gagcgggtca gactcaccga
     5341 agaaagagta aagcttgaag atgttttctg gggcgagaag gatcttcagg gttacttaac
     5401 cgagcttact accagggatg gtaaaaaata taaaggcgaa agtcctgcga cggcgatgga
     5461 catttataaa gacaaacgcc aggtcgcggg aagtatttcc aatatggcgt tggaagttct
     5521 cggagaaacc gggaagacgc ttgaccgcga gtccctggcc tatatgagca aattagagac
     5581 cgagatagcg gaacttcagg cccgtatcga aaattctgaa acttcagagg ctgacaaatt
     5641 gtcggcacaa gcctcattga agggtaaaga atttacgctt gagcgcttta accatgccaa
     5701 agctaaaaaa gaagcggact ataaggtttt tatgggccag tcaggcggca aggatggtaa
     5761 gacatggaca aaaaatgacc ggattcagtc cgtaattgag atgcgtagaa tcatgagtag
     5821 tttacgtact agtgtggaag gactttacgg ttcgttggat acctcagacc cccgcgactc
     5881 gggaattctt cggactctgg cggagttcgt ttatggggat gttcagagtg ggtatatgcc
     5941 tagtgctctg ggttttgacg atccagaaga atgggaaaag tggcaggcca tgagcattga
     6001 ggaacgtctg acggttttaa ctgaccggct tcaaattgcc gggaacctca atcgttcgct
     6061 ccagaaggcg ggaatttccc ttgatctcaa ggacacccat gtcaccaacc ttggcgaaaa
     6121 aggtgaagat gttggcctct tgatgtcgat tgcatttgac cttcaaaaag attcggacgc
     6181 ctgggttgaa ttttttgata agagtggtga gttggccggc aacaccgacg aaactcgtca
     6241 aacagcacgc acgcgagtta ataggctctt tgggcttata cttagcccgg cagggcgtgc
     6301 cattattcag gaatatttgg gaggaccggg cagt
//
LOCUS       DAOSIO010000094         6333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_11124_length_6333_cov_4.221886, whole genome shotgun sequence.
ACCESSION   DAOSIO010000094 DAOSIO010000000
VERSION     DAOSIO010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11124_length_6333_cov_4.221886"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..723)
                     /locus_tag="PK590_04520"
     CDS             complement(34..723)
                     /locus_tag="PK590_04520"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HOG23895.1"
                     /translation="MKPYIVAISGASGVIYGVRLLEILLEQNIPVELVVSDTARVVMR
                     EERSCPEVEKFSVFCETGETPPFFAKKGFAPMTVHGFHDFTASIASGSYPVAGMVIVP
                     CSMGTLGAIASGLSRNLIHRAADCAIKEGRKLVLVPRETPLSAIHLENMLKLARLGVK
                     IVPPMPAFYGAQQTVAEMVDFVVGKVLDQLEIPHALYPRWVGKGSRVSSFMSREDPNL
                     GPDHLAREPME"
     gene            complement(746..1525)
                     /locus_tag="PK590_04525"
     CDS             complement(746..1525)
                     /locus_tag="PK590_04525"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOG23896.1"
                     /translation="MSKLSVYRMRPEHELYVATDYRILYQDPSFLVVDKPAPLPVHPV
                     GRFREKNLLSLLKKDIAFGGDDLRIVNRLDSETSGLVLVGRSAGVTGKLGSLFEKRLV
                     LKEYLAIVLGSPAVDRGTIEVPLGLKGGGVHNIRCFDANGQAAKTDYEVLRRMNGRSF
                     LKVIPRTGRTHQIRVHLSLLGHPVAGDKIYIDPSVFERYIDQGWQEDMRPIVKSERLL
                     LHASRLEFRHPESNEVMTFGSDLPACFHSFLNPGVDLDEIV"
     gene            complement(1529..2380)
                     /locus_tag="PK590_04530"
     CDS             complement(1529..2380)
                     /locus_tag="PK590_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HOG23897.1"
                     /translation="MIKKIKVFFQDIKFEHSLFALPFAFLGLILAERGMPSLFLWVWI
                     TVAMVSFRTFAMGLNRFLDASVDAMNPRTAARAIPSGQLSKRWVGVILIVSALIFEFS
                     AFKLGPLCFLLSPVPFILAAVYPLMKRFSYLSHFVLGSVLAIAPYGAWIASRGSLDLA
                     PTFLSLGVICWVAGFDMVYALQDVEFDRRTGLHSFPVKFGTAVTLTTTRSLHFLSVIF
                     WTLAGYWNGQGVIFFAGILCAAIFLGRSHYLIRSYGIARINESFFVMNVAVSVGLLIA
                     TVLDQVI"
     gene            complement(2455..3924)
                     /locus_tag="PK590_04535"
     CDS             complement(2455..3924)
                     /locus_tag="PK590_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HOG23898.1"
                     /translation="MGYKGLKDFLKILKLEGELVEISAPVDPVLEVTEIYDRVVKKEG
                     PALLFKAPDGSDMPLVINLFGSEKRMAMALGVKKLSQVAERIESLLDSKVPENMWEKL
                     GMLPKLFELNALLPKMVSRAPCQEEIVYQGKGQLLGRLPVLQCWPKDGGKFITLPMVF
                     TKDPLTGKRNVGMYRMQVYDDETTGMHWHVHKDGAEHFRKLTRGKKERLPVACAIGAD
                     PATIFSAACPLPYGMDELMLAGFLRQSPVELVKCKTVDLEVPADAEIVLEGYVEEGEM
                     RLEGPFGDHTGFYSRAKEFPVFHVTCMTHQKNPIYAATVVGRSPMEDCYMGRAIEHIF
                     LPMIRKQFPEIVDMHLPWEGSFHNCVILAIQKRYPEQGKKVIHGVWGLGQLMFSKMVI
                     VLDQDSNIRDMREVAWRVLNNFDPKRDIVSSEGPLDELDHGAPRDFAGSKIGIDATRK
                     TAEEGMERPWPEDIVMDDEIKKRGESRWKEYGLDPRYLS"
     gene            complement(3914..4594)
                     /locus_tag="PK590_04540"
     CDS             complement(3914..4594)
                     /locus_tag="PK590_04540"
                     /inference="COORDINATES: protein motif:HMM:NF016206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOG23899.1"
                     /translation="MNPLQGLLFLLTVGISFIVVLYGAWVKVKYCRPGFLTAITGSEV
                     ARMVLDKMGMFHVSVTPVECSDEAVSSSASEGLFISRKIYDGRDIPSLVRAARGAFLK
                     GQLSNLIFWTHLKKKVTFAAQVTVVLGWICFLAGMLIRNAGFLVTPGLGAFAVVLLLS
                     FYDLSFELDAEDRTSRLMKEARCLEPHEWMCFQRLNRAAALEGLACLVLMPWNLVSGV
                     LRRNCHGI"
     gene            complement(4681..5607)
                     /locus_tag="PK590_04545"
     CDS             complement(4681..5607)
                     /locus_tag="PK590_04545"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOG23900.1"
                     /translation="MTTRGRFDEFIVDEAESQLRLDVFLAAKLSDRHSRAQLQKMIRS
                     GGVLVEGKTVTPHYAVKAGERISVEKLERPRVDLPAEAIPIEILHEDEALIVVNKPAG
                     MVVHPAHGNPNHTLVNALLFHVRNLSHEGDKVRPGIVHRLDKDTSGVMVVAKTDIAHS
                     KLAKQFKDHSIERVYHAIVRGVIQHDEGVIEEPVGRAFLNRKKVIVKPSGGKDAVTYF
                     KVKQRFSRGTLVEIRPQTGRTHQIRVHLAHLGHPVLGDTLYGIPFPSIQRQALHASFL
                     GFEHPDHGKRVSFSSELPRDMKELLSFLETRP"
     gene            complement(5604..>6333)
                     /gene="lgt"
                     /locus_tag="PK590_04550"
     CDS             complement(5604..>6333)
                     /gene="lgt"
                     /locus_tag="PK590_04550"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOG23901.1"
                     /translation="SLGPLKLYSYGLLFAVGMVVSLSLMRRRAVKEGFPAPDDCSDMM
                     LVLLVWGVVGARLFYVLQNLSYYASDPLRVFAIWEGGLIFYGGAVSGLIGFIVTLRMK
                     KIPFWRFLDFIVPYIALTQFFGRIGCFLNGCCFGKTCHLPWAVSFPHSGGPVHPAQLY
                     EAFYTLLLFVCLRVRGRRARFEGEVGLLYFMLYALGRYLIEFVREPGLSWLGFTMNQW
                     ISIVMIVTAFIFFQARKSQGSTRQ"
BASE COUNT         1718 a         1841 c         1439 g         1335 t
ORIGIN      
        1 atcccgatcc gcagggggcg tttcatttta ttctcattcc atcggctcgc gggccaggtg
       61 atccggaccc aaattcgggt cctcgcggga cataaacgac gaaacgcggc ttccttttcc
      121 gacccaccgg ggataaaggg cgtgcgggat ttcaagttgg tccaagacct tcccgacgac
      181 aaaatcgacc atttcggcga cggtctgctg cgcgccgtaa aaagccggca tcggcggcac
      241 gatctttacc ccaagccgcg cgagtttcag catattttca aggtggatag ccgataaagg
      301 agtttcccga ggcaccagga ccagcttgcg accttccttg atggcgcagt ccgcggcccg
      361 gtgaatcaga ttccgggaaa gccctgacgc gatagcgcca agggttccca tactacaggg
      421 aacgatcacc attcccgcga ccgggtacga accgctcgcg atcgaggccg taaaatcgtg
      481 aaaaccgtga accgtcatgg gggcaaaccc ttttttcgcg aagaaaggtg gcgtctcccc
      541 cgtttcgcaa aaaacggaaa acttctccac ctccggacaa ctcctttcct cccgcatcac
      601 gacccgggcc gtatcggaca ccacgagttc caccggtatg ttttgctcaa gcagtatctc
      661 cagcaaacgg acaccgtaaa taacgccact cgcaccgctg atcgccacga tatagggttt
      721 catgtttcct cttaccggtt aaaagttaca cgatctcgtc aagatcaacc cctggattca
      781 aaaaagaatg aaaacaagcc ggcaaatccg aaccaaaggt cataacctcg ttcgattcgg
      841 gatgccgaaa ttccagccgg gaagcatgga gaagcaacct ttccgatttc actatgggtc
      901 gcatgtcctc ttgccatccc tggtcaatat agcgttcgaa aaccgaagga tcgatataaa
      961 ttttgtctcc cgcgaccgga tgaccgagaa gagacaggtg gacccgaatc tgatgagtgc
     1021 ggcccgtacg cggaatcact ttgagaaacg agcgcccatt catgcgtctg aggacctcat
     1081 agtcggtttt tgcggcctga ccgttggcat cgaaacaccg gatattgtga actcccccgc
     1141 ctttcagccc cagaggaact tcaatggttc cccgatcaac cgccggactc ccaagtacaa
     1201 tcgcaagata ttcttttaaa accagacgtt tttcaaacag gcttccgagt tttcccgtca
     1261 ccccggctga tcgccccacc agcacaagac cgctagtttc cgagtcaagg cggttaacaa
     1321 ttcgcagatc atctcctccg aaagcaatat cttttttcaa cagactcagg agattctttt
     1381 ccctgaatcg tccgaccggg tggaccggaa gcggggcggg cttatcgacc acgaggaacg
     1441 aagggtcctg ataaagaatc ctgtaatccg tggcgacata aagttcatgc tcgggtctca
     1501 ttcgataaac ggacaatttc gacatagctt aaatcacctg atccagtacc gtcgcgatca
     1561 acaaccccac actgacagcc acattcatga caaagaaaga ttcattgatt ctggcaattc
     1621 cataggagcg gataagataa tgtgagcggc ctaaaaaaat cgccgcgcac agtatccctg
     1681 caaaaaaaat cactccttgc ccattccaat aaccggcaag ggtccagaaa atcacgctta
     1741 aaaaatgtag ggatcgcgtt gtcgtcagag tgaccgccgt cccaaacttg acaggaaaag
     1801 agtgcaaacc tgtcctccgg tcaaattcca cgtcctgcaa ggcataaacc atgtcaaatc
     1861 ccgccaccca gcaaatcacc ccaagcgaca ggaaggtcgg cgccagatcc aaactgccgc
     1921 ggctcgcaat ccaggcgccg taaggggcaa tcgccagaac acttccaagc acaaaatgcg
     1981 agagatacga aaaccttttc atcagtggat acactgccgc cagaataaaa ggcacgggcg
     2041 aaagcaaaaa acaaagcggc ccaagcttga acgcagaaaa ctcgaaaatc agagctgaaa
     2101 caatcaaaat cacgcccacc caacgcttgg acaattgtcc ggaaggaatg gcgcgtgccg
     2161 cggtccgggg attcatggca tcgaccgagg cgtccagaaa acggttcaac cccatggcaa
     2221 aagtccggaa actcaccatt gctaccgtaa tccagaccca aagaaaaagc gagggcatcc
     2281 ctcgctcggc aagaattaat cccaaaaatg cgaacggaag ggcgaagagc gaatgctcga
     2341 atttgatgtc ctgaaaaaaa actttgattt tttttatcat gcctttgaga tcaatgttcg
     2401 gggcgaatca aaaaaaccag ccgctgaaag accgatgccc ggcgccggca tatgttacga
     2461 caagtatcgg ggatccagcc cgtattcttt ccaacgcgac tcaccccgtt tttttatttc
     2521 atcatccatc acaatatctt ccggccaggg gcgttccatc ccttcctcag ccgtttttcg
     2581 ggtcgcgtcg ataccgatct tggaacccgc aaaatcacgc ggcgcgccgt ggtcgagctc
     2641 atccagcggc ccttcgcttg aaacgatatc gcgtttagga tcaaaattat tcagcacgcg
     2701 ccacgcaact tcgcgcatgt cccggatatt agaatcctga tccaacacaa tcaccatctt
     2761 ggaaaacata agctgtccca gtccccaaac tccgtgaata actttcttcc cctgttccgg
     2821 ataacgcttt tgaatcgcga gaatcacgca attatgaaaa cttccctccc acggcaaatg
     2881 catgtccaca atttccggaa attgcttgcg aatcattggg agaaaaatat gctcgatggc
     2941 gcgccccatg taacaatcct ccatgggaga acgaccgacc accgtcgcgg cataaatcgg
     3001 gtttttctga tgcgtcatgc acgtcacgtg aaaaaccgga aactctttcg cccgcgaata
     3061 gaatcccgtg tgatcaccaa aggggccttc cagccgcatt tccccttctt caacataacc
     3121 ttcaagcaca atttcggcgt cagccgggac ttccaaatca accgttttgc attttacgag
     3181 ctcgacggga ctttgacgca aaaatccagc cagcatcaat tcatccattc cgtaaggcaa
     3241 aggacaggcc gcggaaaaga tggtcgcggg gtccgccccg atcgcgcagg ccaccggaag
     3301 ccgctctttc ttcccccgcg tgagtttacg aaaatgttcc gccccgtctt tatgcacatg
     3361 ccaatgcatg ccagtggttt catcatcgta aacctgcatt cggtacatcc cgacgttccg
     3421 ttttccggtc aaaggatctt tcgtaaaaac catgggaagc gtgataaatt ttcctccgtc
     3481 tttcggccag cattgcaaca ccggcaggcg gcccagaagc tgacccttcc cctgataaac
     3541 aatctcctcc tgacacggcg cccggctgac catcttggga agaagggcgt tcagctcaaa
     3601 aagtttgggg agcatcccca gtttttccca catattttcc ggaactttgg aatctaggag
     3661 agattcgatc cgttctgcca cttgagaaag ttttttaacc cccagcgcca tggccattcg
     3721 tttttcactg ccaaacaaat taatcacaag cggcatgtct gatccgtcag gagctttaaa
     3781 caaaagcgcc gggccctctt tcttgacgac ccgatcataa atctcggtca cctcaagcac
     3841 cggatccacc ggcgccgaga tctccaccaa ttcaccttca agtttgagga tcttcaaaaa
     3901 gtctttgagc cctttatatc ccatgacaat ttctccttaa cacgccactc acaagattcc
     3961 acggcatcaa gaccagacag gctaaccctt ccagggccgc ggcccgattc agcctttgga
     4021 aacacatcca ttcatgcggc tccagacaac gcgcctcctt catgagcctc gaggttcgat
     4081 cttcggcgtc caactcaaac gaaagatcat aaaaagacag gagcaagact acggcaaatg
     4141 cgcctaatcc aggcgtcacc agaaaccccg cgtttctaat caacataccc gccagaaagc
     4201 agatccatcc caatactacc gtcacctgcg ccgcgaatgt aactttcttt ttaaggtggg
     4261 tccagaaaat cagatttgaa agttgcccct tcagaaaagc cccgcgcgcg gcacgcacga
     4321 gagaagggat atcccggccg tcataaattt ttcgagagat aaaaaggcct tcggacgccg
     4381 atgaactcac cgcttcgtcc gaacactcaa ccggagtcac gctgacatga aacattccca
     4441 tcttgtccag aaccatcctg gccacttcag aaccagttat ggcggtaaga aaaccgggcc
     4501 ggcaatactt cactttaacc caagcgccgt agaggacaac gatgaaagaa atacccaccg
     4561 tcaataggaa caacaaccct tggagaggat tcacccttta agatctccgg ttagtctttg
     4621 attgaaaaaa tgattataag cgaaccgtcg cggtgactct acgctttttt ggaagaaaaa
     4681 ctacgggcgg gtttctagga aagaaagaag ttccttcatg tcccggggaa gttcacttga
     4741 aaaagagacc cgcttcccat ggtcgggatg ttcaaacccg aggaaggatg cgtgaagggc
     4801 ctgacgctgg atcgacggaa aaggaattcc gtaaagggta tcccccagca ccggatgccc
     4861 cagatgagcc aggtgaaccc ggatttggtg tgttcttccg gtctggggcc gaatttcaac
     4921 gagagttcct ctcgaaaaac gttgtttgac cttaaaatag gtaacggcgt cttttccccc
     4981 cgaaggtttc acaatcactt tttttcggtt cagaaaagca cgccctaccg gctcctcaat
     5041 caccccctcg tcatgctgga tcacgccccg gacaatcgcg tggtacaccc tctcgatcga
     5101 atgatcttta aactgcttcg caagttttga gtgggcgata tccgttttcg ctaccaccat
     5161 cacgcctgaa gtgtccttgt ccagtcggtg aacaatcccg gggcgaacct tgtccccttc
     5221 atgggacaga ttccgcacat gaaaaagcaa cgcgttgacc agtgtgtggt tggggttgcc
     5281 atgggcgggg tgaaccacca ttccagccgg cttgttaacc acaatgagag cctcatcttc
     5341 atgcaaaatc tcgatcggga tggcttccgc gggaagatcc actcgtggtc tttcaagctt
     5401 ttcaaccgaa attctctctc cggccttcac ggcgtaatgg ggagtcactg tttttccttc
     5461 cacgagcacg ccgccggaac ggatcatttt ttgaagttgc gcgcgggaat ggcgatccga
     5521 aagtttagcg gccagaaaaa catccaggcg taattgagac tcggcctcat ccacaataaa
     5581 ttcgtcaaac cggcctcgcg tcgtcactgg cgcgtactcc cctgagactt gcgcgcctga
     5641 aaaaaaataa acgccgtcac aatcatcacg atgctaatcc actgattcat cgtaaaaccc
     5701 agccagctca gtcccggctc cctgacaaac tcgatcaggt aacgccccag ggcatacagc
     5761 atgaaataca gcaacccaac ttccccttcg aatcgggccc gtcgtcctcg tacacgcaaa
     5821 cagacaaaaa gaagcagtgt gtagaacgcc tcataaagtt gcgccggatg aacgggacct
     5881 cccgaatgcg ggaaactgac cgcccacggc aaatggcaag tctttccaaa gcaacatccg
     5941 ttcaaaaaac atccgatacg cccaaagaat tgcgtcaagg caatgtaggg tacgataaaa
     6001 tcaagaaatc gccagaaggg aatttttttc atccggagag taacgatgaa tccgatcaat
     6061 ccactgacgg cccctccgta aaaaattaat cctccttccc aaatcgcaaa aactctgaga
     6121 gggtccgaag cgtaatagga caggttttga agaacataaa aaagtcgcgc ccccacgact
     6181 ccccacacca aaaggacgag catcatgtcc gagcagtcgt ccggcgccgg gaagccttcc
     6241 ttcacagccc tgcgacgcat caggctgagc gaaaccacca tgccaaccgc aaaaagcagt
     6301 ccgtagctgt aaagtttcaa gggaccgagc gag
//
LOCUS       DAOSIO010000095         6208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_11421_length_6208_cov_5.255485, whole genome shotgun sequence.
ACCESSION   DAOSIO010000095 DAOSIO010000000
VERSION     DAOSIO010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11421_length_6208_cov_5.255485"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..1592
                     /locus_tag="PK590_04555"
     CDS             138..1592
                     /locus_tag="PK590_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOG23902.1"
                     /translation="MSWILILALGLGGCTMIPKYTRPDAPVPEQFPSGAAYASLPASA
                     DVSSNVTRIRWQEFFTDPKLQKIIEMALQNNRDLRLVSLNVERAQGLYGIRRAELLPV
                     GNADAGGGEKQVSADFVGPGVKRTSKAYEVNLGVAAWEIDFFGRIRSLEKQALQDYLG
                     TEQARRSAQISLVSSVANAYLMLAAERENLALAEDTLESQTATCGLVQRQYELGIVTQ
                     LDFHRAKIPVEIARHDVARYKELVAKGRNALDLLAGAPVPEDLLPANLADIHPTREII
                     AGVSSEVLLNRPDIIAAEHHLKSAYANIGAARAAFFPRISLTTTLGSASADLSGLFKA
                     GTATWLYAPQLVMPIFDARTWSAYRVSRADQKIALTQYEKTIQTAFREVADALATNGT
                     IGERVAAQQSLVDTARETYRLANSRYSMGIDSYLSVLDAQRDLFSGEQSLIYLRQAKA
                     ANQVHLYAVLGGGAEDEGSGNISLRKRISRFFRF"
     gene            1781..3619
                     /locus_tag="PK590_04560"
     CDS             1781..3619
                     /locus_tag="PK590_04560"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOG23903.1"
                     /translation="MKKFFMILLILLVIAVIGVGVFLATFDADQYRPQILGLIEKAIQ
                     KPVRLEKITLGWKSGMALELQGFAILKSNQSQEKLVEVQSAKAVLKLVPLLSKRIQVA
                     AIHLIHPVIRLVKNPDGTFEGWEPEPAPSGETQEKADSGPEPAALLSLLVNEIRVQDG
                     ELFFKDRSGKESMELDLRKIDVMVSDVELNRPINFQVNAAVLSKEQNLEVKGKLTLAP
                     QTRSGTVSGLRVELQLAPIDFQEVIRLSPDLKTSGIVFPLGGTLTAESDSLKIDNEKL
                     INNGVKILLQRGKIHFETLRKPIENISLGAWMTNEMIEVQQFSASVAAGKVEGQGAVN
                     LADSKNPRTDFDVKADKISIEELTPDYSSGPQVRGLLSAAMRGKLSGQTAEEMLRTLT
                     ADGSVKLDQAVIARMNVLREVFQKMSMIPGLVERLLTRLPKNYQEKLKSEDTKLEMAQ
                     IPFSVQNGLLHLPRLEIATDSFVAAGSGTYGLNEGSVTGKVLLAIEPDLSSAMTRSVE
                     ELQYLTNEKKEIQIPLVIQGLIPKVVVMPDVSHVASKMAAQKAREVLGGYLEKALAGK
                     NNAPQNQPVSADAGSGESAVAKPSSGGRGLLEALLQQGIRESGSGQ"
     gene            3904..4272
                     /locus_tag="PK590_04565"
     CDS             3904..4272
                     /locus_tag="PK590_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23904.1"
                     /translation="MKKTTVVLLLLAMLAMPAAGFAASPWTEETEYGDKISEKLQFGL
                     KNTLLGWTDIFFEPIRASKKCGSCDSIWTGLGKGLTDAIVNEVGGAFHLLTFPLVVDF
                     PLPDDGVQFATCCGCGSNGK"
     gene            complement(4488..5825)
                     /locus_tag="PK590_04570"
     CDS             complement(4488..5825)
                     /locus_tag="PK590_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HOG23905.1"
                     /translation="MIQKTLRFFKYDIWRIRVREAKGFRGKCIRMVRIFLISFRQFSG
                     DMCSLRAAALTFFSLLSIVPVLAMSFGIAKGFGMDKLLREKLIEEMHGQQEVLIRIIT
                     FAESLLENTKGGVIAGVGVALLLWSVIQVLGNIETSFNGIWGIKKQRSLSQKFTDYVA
                     MMLIAPVLYIVASSASVFAASRITAITQNTAFLGAVAPLIMTGLKILPFLIFGGLLTY
                     LYIAMPNGKVHFKSALLGGMVAGIMYHIVQWIYIRFQIGASRAGAIYGSFAALPLFLI
                     WLQTSWRVVLYGAELAFSHQNEQTFEFEQDCLNASYEIRKLLSLRIVQLCIARFTEGL
                     PPLSAEEIAAELEMPIRLARDLIFRLIAANILTGAQSDTERERRYQPARDIGDMTIQF
                     VIDQMEKTGSKEIPVAHTSELENLRKSLSVFEQTIRNLPENKLLKDLVPVRAS"
     gene            5991..6068
                     /locus_tag="PK590_04575"
     tRNA            5991..6068
                     /locus_tag="PK590_04575"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6027..6029,aa:Glu,seq:ttc)
BASE COUNT         1454 a         1500 c         1769 g         1485 t
ORIGIN      
        1 gctactttta ttgacctgat ttttattccg ctgtttttcg tggtgatctc ccgtgttttt
       61 ggaagaaaac ttgcgcggat cgacggggcc aatgtcacgg atacggtgac gccggagacg
      121 acatcatgaa ccggcgaatg tcctggattc tgattttggc gctggggctc ggcggttgta
      181 cgatgatccc aaaatacacg aggccggacg cgccggttcc ggagcaattc ccgagcggtg
      241 ccgcttacgc gtcattaccg gcgtcggcgg atgtttcctc gaacgtcacg cggatcagat
      301 ggcaggaatt cttcaccgat cccaaacttc aaaaaatcat tgaaatggcc cttcagaata
      361 accgggactt gcggcttgtg tccttgaatg tggaaagggc gcaaggtctc tacgggatcc
      421 ggcgggcgga gttgcttccg gtgggcaatg ccgatgccgg gggaggtgaa aagcaggttt
      481 ccgccgattt tgttggcccc ggagtcaaga ggacttccaa ggcgtatgaa gtgaacctcg
      541 gggtcgccgc ctgggagatt gatttttttg gcaggattcg cagtctggaa aaacaggctt
      601 tgcaggatta cctgggtacc gagcaagccc gtcgcagcgc gcaaatctcg cttgtgtcgt
      661 ccgtggcgaa cgcgtacctg atgctggcgg ctgaacgtga aaaccttgcc ctggcggagg
      721 atacgcttga aagccaaacc gccacttgcg ggctggttca gagacaatat gaactcggga
      781 tcgtgaccca acttgatttt catcgcgcca aaattccggt tgaaatcgct cgacatgacg
      841 tggctcgcta caaggagctt gtggcgaaag gtcgaaacgc gctggatctt ctggcggggg
      901 ctccggttcc ggaagatctc ttgcctgcca atctcgcgga tatccatccg acccgggaaa
      961 tcatcgcggg cgtgtcttcc gaggtgcttt tgaaccggcc ggacattatc gccgcggaac
     1021 atcatctcaa aagcgcttat gccaacattg gcgcggcccg ggccgctttt tttcccagaa
     1081 tttcactgac caccacacta ggctccgcga gcgcggatct ttcaggttta ttcaaggcgg
     1141 gaaccgccac gtggctttac gcgcctcaat tagttatgcc gatctttgac gcccgcacct
     1201 ggtctgctta cagggtgagt agggcggacc aaaagatcgc tttaacccag tatgaaaaga
     1261 caatccagac ggctttcagg gaagtcgcgg atgccttggc gacaaacgga acgatcgggg
     1321 aacgggtggc ggcgcagcag tcgctggtgg acactgccag ggagacttac cgcctggcga
     1381 attcccgtta ttccatgggg attgacagtt atctgagtgt cctggacgct caacgggatc
     1441 ttttttcggg agagcagtcg ttaatctacc ttcgccaggc gaaggccgcc aatcaggtcc
     1501 atctttacgc cgttctgggc ggcggcgcgg aagatgaggg gtccggtaac atttctctgc
     1561 gaaagagaat ttcccgcttt ttccggtttt aactttacct cgattttccc ttcccgaatt
     1621 ccggacatcg cgcaaaagat tatcatcttt tcttgattct cgtcctggtt cgtcgtagtt
     1681 tacgcagagt cgacgacctt tcttgtcggt ggtcttgtca aaaatattcc aaaaagcgac
     1741 gaatgtcctg aacgggcgta atgcctgaaa ggaagctgaa atgaaaaagt tttttatgat
     1801 tcttttgata ttgctggtga ttgcggtgat cggggtgggc gtctttctgg cgacctttga
     1861 cgcggaccaa taccggccgc aaattctggg cttgattgaa aaggcaatcc agaaacccgt
     1921 gcggttggaa aagattacac tcgggtggaa gtcgggcatg gcccttgaac ttcaggggtt
     1981 cgccatcctg aagagtaatc agagtcagga gaagcttgtt gaggtccagt ccgcgaaagc
     2041 tgttctcaaa cttgtcccgc ttctttctaa aaggattcag gtggccgcga tccatttgat
     2101 ccatcccgta atccggcttg tcaagaatcc ggacggtaca tttgaaggtt gggagccaga
     2161 accggcgccg tcaggtgaaa cccaggaaaa ggccgactcc ggtccggagc ccgcggcgct
     2221 cctttcgctc ctggtgaacg aaatcagggt acaggacgga gagcttttct ttaaggatcg
     2281 ctccgggaaa gagtcgatgg aactggatct tcgcaagatc gacgtgatgg tgagtgatgt
     2341 cgagctgaat cgcccgatca attttcaggt caatgcggcc gtcttgagca aggaacaaaa
     2401 tctggaggtg aaggggaaac tgactcttgc gcctcaaacg cgtagcggca ccgtcagcgg
     2461 attgcgcgtc gagttgcaac tggccccgat cgattttcag gaggtaatca ggcttagccc
     2521 ggatttaaaa acttcaggga ttgtttttcc tcttggcgga accctgacgg ccgaatccga
     2581 ttcgttaaaa atcgataatg aaaagctgat caataacggc gtgaaaattc tgcttcaacg
     2641 cggaaaaatc cattttgaaa ctctccggaa gcccatcgaa aacatttctc tgggggcctg
     2701 gatgacgaat gagatgattg aagttcagca gttttcggcc agcgtcgcgg ccgggaaagt
     2761 tgaaggacaa ggcgcggtga acctggcgga ctctaaaaat ccaagaacgg attttgacgt
     2821 caaggcggac aagatttcaa ttgaggaact gacacccgat tattcttcgg gtccgcaagt
     2881 caggggattg ctttcggccg ccatgagggg aaaactgagc gggcagacgg cggaggaaat
     2941 gttgcgaacg ctgacggcgg acggatcggt gaagctggat caggccgtga tcgcccgcat
     3001 gaatgtcttg cgcgaggttt ttcaaaagat gtcaatgatc cccggacttg tggaaagact
     3061 tttgacgcgc ctgcccaaga actaccagga aaaattaaag agtgaggaca cgaaactgga
     3121 aatggcgcaa atcccatttt cggtccaaaa cggtctgctg catttgcctc gtctggagat
     3181 tgccacggat tctttcgtgg ctgccggctc cgggacgtac gggctgaacg aggggagcgt
     3241 gaccgggaag gtccttctcg cgatagaacc ggatctttcc tcggcaatga cccgcagtgt
     3301 ggaggagttg caatatctga cgaatgagaa aaaagaaatc cagattcctc ttgtgatcca
     3361 gggcctgatt cccaaagtcg tggtgatgcc ggacgtttcg cacgttgcct cgaaaatggc
     3421 ggcccagaag gcccgggaag ttttaggggg ttatctggaa aaggccctgg ctggaaaaaa
     3481 taatgctcct cagaatcaac cggtttcagc cgacgcgggt tccggggaat cggctgtggc
     3541 caagccttcg tctggaggga gagggttact tgaggcattg ctccagcagg ggataagaga
     3601 atccggttct ggacaatagt ctcaacccgg gttcgggaaa cacgggccgg gtttgaaatt
     3661 tctctaatgc tattggacaa gatgacgata actagtctgt tttacgagtc aggatgcccg
     3721 cggaaaatcc cggcttcagg acatggggac agttttttaa atgggctaaa tcccgcacaa
     3781 aaagccggcc ggatgttcgc ttgtttgatc cggttttacc tcggtgctag acccgaggcc
     3841 cgttttcagg gcttgggtag tttcagtttt ttaacgcggt aaccaaaacc aaaggagaaa
     3901 aacatgaaaa aaacaacggt tgtgcttttg ttgttggcga tgctggcgat gcccgcggca
     3961 ggtttcgcgg cgagcccctg gacggaagaa acagagtatg gtgacaaaat ttccgaaaaa
     4021 ctccagtttg ggctgaaaaa cactctcttg gggtggacgg atattttctt tgagccgatt
     4081 cgcgcttcaa aaaagtgtgg tagctgtgac agcatctgga cgggacttgg caaaggtttg
     4141 accgacgcga ttgtgaatga ggtcggaggg gcttttcatt tgctgacatt tccgttggtt
     4201 gttgatttcc ctcttccgga cgacggagtt cagtttgcca cttgctgtgg ttgtggctcg
     4261 aacgggaagt agggcacacg gttttaatcg gctcatctcc caggtgaata cgggatgatg
     4321 ggaagttgaa cgacaagaag tttcggcgca cttttttcca ctcaggtgga agagagtgcg
     4381 ccgttttttt tgaggaaatt agcgtgcaat gaagcgtatt caaaaaggaa gctggcggcg
     4441 ggtcaaaact ggaacgggga accctgtcct ttccaaattc tctcttttca ggaggcgcga
     4501 accgggacga ggtctttcag gagtttgttt tcgggcaagt tccgaatcgt ttgctcaaag
     4561 acagacaatg atttccgcag attttcaagt tccgacgtat gggcaaccgg gatctctttc
     4621 gagccggtct tttccatttg atctatgacg aactgaatcg tcatgtctcc gatatcacga
     4681 gccggctgat aacgacgctc gcgctccgtg tcgctttgcg cgccggtcag gatgttggcc
     4741 gctatcagtc gaaagatgag gtcgcgggcc agccggattg gcatttcgag ttccgcggcg
     4801 atttcctcgg ccgaaagggg aggtagtccc tcggtaaaac gggcgatgca aagctggact
     4861 atccgaagag acagaagttt tctgatttca taactggcgt ttagacaatc ctgttcgaac
     4921 tcgaaggttt gctcgttctg gtgagagaac gccagctccg cgccataaag gacgacccgc
     4981 cagcttgtct gtagccagat caggaaaagc ggaagggcgg caaagcttcc gtaaatagcg
     5041 ccggctctgg acgctccgat ttgaaaacga atatagatcc attggacgat atggtacatg
     5101 atcccggcga ccattcctcc gagtagagcc gacttgaaat ggacttttcc gtttggcatc
     5161 gcgatgtaaa gataggttag cagtccccca aagatcagaa agggcaggat cttgagccct
     5221 gtcatgatga ggggcgcgac cgcgcccagg aaagccgtgt tctgcgtgat ggctgtaatc
     5281 cggctcgcgg cgaatactga ggcactgctc gcgacgatat agagaacggg cgcgatgagc
     5341 atcatggcga cgtaatcggt aaatttctgg ctcaggctcc gctgtttttt gattccccag
     5401 ataccgttaa aggaggtttc gatgttcccc aatacctgga tgacggacca gaggagaagc
     5461 gcgacaccca cgccggcaat cactccgcct tttgtgtttt ccagcaggga ttccgcgaat
     5521 gttatgatcc ggatcagcac ctcctgttgg ccatgcattt cttcaatcag cttttcacgc
     5581 aggagcttgt ccatgccgaa acctttggcg atcccgaacg acatggcgag caccggaaca
     5641 atggaaagaa gtgaaaagaa ggtaagggcc gccgcgcgaa gcgagcacat gtcgcctgaa
     5701 aactgacgaa aggaaatcag gaagattcga accatgcgaa tacacttgcc ccggaagcct
     5761 ttggcctccc tgacacgaat tcgccaaata tcgtatttaa aaaaccgcag ggttttttgt
     5821 atcatgcggg ggattgtagc ataaagtcat ccgatcccta aaaacatttc cataatctca
     5881 ggtttagtta tgaaacagga acctttcctt ctcccagcgc gccccgggtt tcctcttgga
     5941 tttttggttg aaatcggtat aattcaccac ctcggactca tcccctgcgc gtccccgtcg
     6001 tctagcctgg cccaggacac cagattttca ttctggtaac acgggttcaa atcccgtcgg
     6061 ggacgccaaa cttgattctt gagccgcgga aacttgtaac gtcttgaaaa atatacaggt
     6121 ttctgcgatt ttaaattccg gggcaggttg ttgttgctat tttgctggat tgactggtga
     6181 tgcgaaatta tactgtagcc cctgcgtt
//
LOCUS       DAOSIO010000096         6202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_11441_length_6202_cov_3.545957, whole genome shotgun sequence.
ACCESSION   DAOSIO010000096 DAOSIO010000000
VERSION     DAOSIO010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11441_length_6202_cov_3.545957"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /gene="folE"
                     /locus_tag="PK590_04580"
     CDS             complement(<1..389)
                     /gene="folE"
                     /locus_tag="PK590_04580"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HOG23906.1"
                     /translation="MAKKVVKKRSEADPFPKLVEQMLRLLGEDPGREGLLRTPVRVAG
                     SLRYLTEGYRTDPKEILHEAVFKEPYDEMVIVKDIEIFSLCEHHLIPFYGKAHVAYIP
                     NGKVVGLSKIPRLVEVFSRRLQIQERLT"
     gene            complement(370..1464)
                     /locus_tag="PK590_04585"
     CDS             complement(370..1464)
                     /locus_tag="PK590_04585"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOG23907.1"
                     /translation="MSTSQHLTREKVMECLKRVGEPLLKKDMMSLGLVREISIDETRV
                     FLHMEDPSGDSKMLDALKAEIGRVLQESGATSVEIQFSGKSQGGKSTEFRPGPIRGVK
                     QVIAVASGKGGVGKSTVAVNLALALAKMNSKVGIMDADIYGPNIPLMLGVRDDQQPEG
                     TTENKILPLEAHGIKVISIGLFTDPDKPIIWRGPLLHKTIQEFLHRVEWGELDYLVVD
                     LPPGTGDVQLSLSQWTHVSGVVVVTTPQEVALSDVRRAVNMFRQLEIPILGIVENMTG
                     PVFGKGGGEKMAEDFQIPFLGDIPLDAQVRKCGDSGVPLVAGDAKEGVCSRFLNIAEA
                     LRESFYKNIKTDSAPDEESDKEYFYGQESS"
     gene            complement(1492..2361)
                     /locus_tag="PK590_04590"
     CDS             complement(1492..2361)
                     /locus_tag="PK590_04590"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HOG23908.1"
                     /translation="MKGFIRFLVIFGSILLLSAFLTPFLYDFLPFKFERIFNRLVMIG
                     TLLAIVIFVRIKKETLRRFGLLWEKNSLSLLAMGFLAGILTLLLTSALNIVFNNARFV
                     IPSLSLMDWVSKVSLALATGLLIGVIEEFFFRGFIFRFLQKAFKNRVLMAVLVTCVFY
                     SLIHFIGMKKIFIGPDPDFIDGLKLIGAPFVSLAEWPRFWPEAVGLFLFGFALNTAAY
                     KSGSLYPSIGLHAGCVFFIRLDDLFIKFQGERTMFWGSKLVYDGVIGWVFLMLLACIL
                     YRTLRPSSVAAKS"
     gene            complement(2407..2862)
                     /locus_tag="PK590_04595"
     CDS             complement(2407..2862)
                     /locus_tag="PK590_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOG23909.1"
                     /translation="MNIESKLLELGLSLPPAPKPVGLYKPFVVSGSLVFLSGQISRDR
                     NGEILAGKVGDDRTIEEGKKAAELAVLNALSVVHHLVGSERIARIVKMNGFVQTAPDF
                     HEVPAVLNRASEILGAVLGEAGSHARSAVGVASLPMNATVEIELILELK"
     gene            complement(2965..3681)
                     /locus_tag="PK590_04600"
     CDS             complement(2965..3681)
                     /locus_tag="PK590_04600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HOG23910.1"
                     /translation="MKDKSYTAPEKAFIQSSFNSITPRYDFLNQALSFGMSERWRQKS
                     CEILLGGEVGDRPRAKTILDLGCGTGKFLECFLKARKWDFAVGLDFSSEMLKKARETV
                     EGAAVWIEEDFEKLPFLEGSFDLIISAFTLRSVQDVPAFLKEINRVLSPGGRVALLDL
                     TRPRGITAFFFYPYLRWILPALGGIISGNRQAYHFLSSSVRGFQRPEKTVDLMENAGL
                     RNIRLKRFAFGVATLIIASK"
     gene            complement(3678..4196)
                     /locus_tag="PK590_04605"
     CDS             complement(3678..4196)
                     /locus_tag="PK590_04605"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG23911.1"
                     /translation="MQRRSHQEVQIEPVLDTVQMAEVARLASLIWRECYAGIVSSEQI
                     DFMLKNFQSERAIAEQIQRGYRYFQLSRGMEILGYFAIQPQAEGRLFLSKIYLKVGER
                     GKGYGRKMMTFIERWAEREGYSEVTLTVNKQNNRAIRAYENMGFRIAESIIQDIGEGF
                     VMDDYGMTKRLS"
     gene            complement(4232..5329)
                     /gene="mqnC"
                     /locus_tag="PK590_04610"
     CDS             complement(4232..5329)
                     /gene="mqnC"
                     /locus_tag="PK590_04610"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HOG23912.1"
                     /translation="MNSHQQILRKRLAGERLTLEEGVELFSCDLLDLGMAAQKLTPRV
                     SPRAVTFVVDRNISYTNVCTIGCDFCAFHCPPGSPEAFVLPLDVILRKVQELADEGGT
                     QVLIQGGIHPDLELDDYLAMVRTIHEKFPQIQIHSFSAIEMDILSKKSGVELPDLFRM
                     FKAAGLNSLPGGGAEILVERVRQKISPKKISAERWLEVMAMAHEAGLKTTTTMVFGHI
                     ETKEERIVHLLKLRELQDKTNGFRSFIPWSFISTNTAMQGIRSTGGDDYLRTVAISRM
                     MLDNIEHIQAGWLTEGMKLAQIALAFGCDDMGGTLMEDKVLEPTGIKVTTRREDLIRL
                     IRDAGFVPVQRNTDYEVLKTFDEADESIHLK"
     gene            complement(5402..>6202)
                     /locus_tag="PK590_04615"
     CDS             complement(5402..>6202)
                     /locus_tag="PK590_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HOG23913.1"
                     /translation="LNCLPFYFELEKKCGEQGLETSWMEASLADLNRALAAEEIDLAP
                     VSSMEYLLRQKDYFLLPGIGIGSDTFVRSVILFSKVPLNELNGETIAVSRESLSSVTM
                     LKILMKRRLGFKNEWVSVPQDIDDAFQRYRAALMIGDVALFYEPENWVYKYDLGKLWR
                     EWTDTPFVFSLWTLRRDCASECGAELRKFCTLLKENRSRHLCEPERLLKNALGVMPDE
                     KKFVSMLGYFVNLRYDLDEEMIRGLLRFYDLAFRENLAPEPKPLEFFE"
BASE COUNT         1676 a         1759 c         1294 g         1473 t
ORIGIN      
        1 gtgagccgct cctggatctg gagcctcctg gaaaacactt ccacgagccg ggggatcttg
       61 gaaagaccta cgactttccc gttcggaata taggccacat gagctttccc gtaaaaaggg
      121 attagatgat gttcgcagag tgaaaaaatc tcaatatcct tcacgatcac catttcatcg
      181 taaggttcct tgaaaacggc ctcgtgaagg atctctttcg gatcagtccg gtatccttca
      241 gtaagatagc gaagagaccc tgccacgcgc accggagttc tcaacaatcc ttcccgcccg
      301 ggatcttcgc cgagcaaacg gagcatttgc tcaaccaatt tcgggaaagg gtcagcttct
      361 gaacgttttt taactacttt cttggccata aaaatactcc ttatccgatt cctcgtcagg
      421 cgcggaatcg gtcttgatgt ttttataaaa agattcccgt aaagcctccg ctatgtttaa
      481 aaagcgggag caaactcctt ccttggcgtc ccccgccaca agcgggacac cggaatctcc
      541 acacttccga acttgagcat ccagcggaat gtctcccaaa aacgggattt gaaaatcttc
      601 cgccattttt tctcctcccc cttttccaaa cacgggaccc gtcatgtttt cgacaatccc
      661 caggatcgga atctcaagtt gacgaaacat attgaccgcc cttcggacat cgcttaaggc
      721 gacctcttgc ggcgtggtga caacgaccac gcctgaaaca tgcgtccact gtgacaggga
      781 aagctggacg tcccctgtcc cgggcggaag atccaccacg agataatcaa gttcccccca
      841 ctccacacga tgtaaaaatt cctgtattgt cttgtgaagc aacggtccgc gccagatgat
      901 gggcttatca ggatcggtaa acaacccgat ggaaatcacc ttgatcccgt gtgcctccag
      961 cggcaagatc ttgttctccg tcgtcccttc cggctgttga tcatctcgca ctcccagcat
     1021 caaaggaatg ttgggcccgt agatatcagc gtccatgatg cccaccttag aattcatttt
     1081 cgcgagcgcg agagcgagat taacagccac cgtggatttt cccacgcccc cttttccgct
     1141 cgcgaccgcg atcacctgct tgacacctcg tatgggcccg gggcgaaatt ccgtcgactt
     1201 accgccctgt gattttcccg aaaactggat ttcgacactc gtcgcgcccg attcctgaag
     1261 cacacgcccg atttcagcct taagcgcgtc gagcatcttc gaatctcccg aagggtcctc
     1321 catgtgaaga aacacacggg tttcatcgat gctgatttct ctcacaagtc cgagagacat
     1381 catatctttt ttcagcaggg gttccccaac tcgcttgaga cattccataa ctttttcacg
     1441 cgttaaatgc tgggaagttg acattcgaca cctctcattt aatttttcag ctcaggattt
     1501 tgccgccacc gaagaaggcc tgagcgttcg atacaaaata cacgcgagta acataagaaa
     1561 cacccacccg atcactccgt cataaaccag cttggatccc caaaacatcg tccgttcacc
     1621 ctggaatttg ataaaaaggt cgtccagacg gatgaaaaag acgcatcccg cgtggagacc
     1681 aatcgaggga taaagagaac cgcttttata ggccgctgta ttcagggcaa atccgaagag
     1741 aaaaagcccc acggcctccg gccaaaatct cggccactca gcgagcgaaa caaagggcgc
     1801 cccaatcagc ttcaacccat cgataaaatc cggatccgga ccgataaaga tttttttcat
     1861 tccaatgaaa tggatcaggg agtaaaaaac acaggtgaca agaaccgcca tcagaacacg
     1921 attcttaaac gccttctgaa ggaaacggaa aataaagccc cggaaaaaga actcctcaat
     1981 cacaccgatc agaagtcccg tcgccagcgc gagcgagact ttcgagaccc agtccatcag
     2041 ggatagtgac gggatcacga atcgcgcgtt attaaaaaca atattcagcg cgctggtgag
     2101 taacaatgtc agaattccgg ccaaaaaacc catcgccaaa agagagagac tgtttttctc
     2161 ccatagcagg ccaaaccggc gcaacgtttc ttttttgatc ctgacgaaaa tgacaatcgc
     2221 gagcaatgtc ccgatcatca ccaatcggtt gaaaatcctc tcgaatttaa acggaagaaa
     2281 atcataaaga aagggggtga gaaaagcgct taagagcaag atacttccaa aaattacgag
     2341 aaaacgaatg aaacctttca tgatgtaaaa ctcctccccc ggccaaggga ccgtttttgt
     2401 tcccttttat tttaattcga gaatcagttc aatctcaact gtggcattca tcggaaggct
     2461 ggccaccccc accgcggagc gggcatgact tcccgcctct cccaataccg ctccaagaat
     2521 ttccgatgcc cggttcagga cggcgggaac ttcgtggaaa tccggcgccg tctggacaaa
     2581 cccgttcatt ttgactatgc gcgcgatcct ttctgaccct acgagatgat gcacaacact
     2641 cagggcattc aacacggcaa gctcggccgc ctttttcccc tcttcaatcg tccggtcatc
     2701 tcccaccttc cccgccagaa tttctccgtt ccgatccctg gaaatctgtc cgctcaggaa
     2761 tacgagactg ccagagacca caaaaggctt ataaagtccg acaggttttg gcgcgggagg
     2821 taacgaaagc cccagttcaa gcaatttcga ttctatgttc ataatatcca ctaatcccaa
     2881 aacattcccg taatttgccc cttgctattt tttcaccaac aaaaccagct gagaaaataa
     2941 ttcgtggggg cctcaaaatg aaaatcattt tgaggctatg ataagcgtcg ccacgccaaa
     3001 agcaaaacgt tttaatcgga tattcctcaa tccggcgttt tccatcaaat ccaccgtctt
     3061 ctcgggtcgc tgaaaacccc gcaccgatga ggacaaaaag tgataggcct ggcgatttcc
     3121 cgaaatgatc cctccgagcg cgggcaaaat ccacctgaga tacggataaa aaaagaaagc
     3181 ggtgatacct cggggacgtg tcagatctaa aagggccacc ctcccgccgg ggcttaaaac
     3241 gcggttaatt tctttcaaaa atgccggtac atcctgaaca cttcgcaacg taaaagccga
     3301 aataatcaga tcaaaactgc cttccagaaa aggaagtttt tcaaaatctt cttcaatcca
     3361 gaccgctgcc ccctcaactg tttcgcgcgc cttcttaagc atttcagaag aaaaatccag
     3421 cccaaccgcg aaatcccatt ttctcgcttt caaaaaacat tccagaaatt ttccggttcc
     3481 gcacccaaga tcaagaatgg tcttcgcccg cggccgatca cccacctccc cgccaagaag
     3541 gatttcacag gacttctgac gccagcgttc cgacattcca aagctcaggg cttgattcag
     3601 gaaatcgtaa cgtggcgtaa ttgagttaaa agagctttga atgaatgcct tttccggcgc
     3661 cgtataactt ttgtctttca tgacaacctc ttcgtcatcc cgtaatcatc catcacaaac
     3721 ccctcgccaa tatcctgaat gatcgattca gctatccgga atcccatgtt ttcgtaagcc
     3781 cgaatagctc tattattttg tttattcaca gtcagagtga cttcggaata cccttcgcgc
     3841 tcggcccatc tctcgataaa agtcatcatc tttcttccgt acccttttcc acgttccccg
     3901 accttgagat agattttgct caaaaaaagt cgtccctccg cctgtggctg tatcgcgaaa
     3961 tatccgagaa tctccattcc ccgcgacaat tgaaagtaac gatacccccg ctgaatttgc
     4021 tccgcgatcg cccgttcgga ttggaagttt ttcagcataa aatcaatctg ttcagaggag
     4081 actatccccg cgtaacattc acgccatatc agggaagcaa gtcgggctac ctcggccatc
     4141 tgaaccgtgt ccagaaccgg ttcaatctga acctcctgat gggaccttct ctgcatcgtt
     4201 caatcacccc tgaaaaaagt agacaattgt tttattttaa gtgaatactc tcatccgctt
     4261 catcaaaagt ttttagtact tcataatccg tatttcgttg gacgggaaca aatcccgcat
     4321 cacggatcag ccggatgaga tcctccctgc gggtcgtcac cttgatcccg gtcggctcaa
     4381 gaaccttgtc ttccatcagc gttcctccca tatcatcgca tccgaatgcc agagcaattt
     4441 gagcgagttt catgccctcc gtgagccaac ccgcctgaat atgttcaatg ttgtccagca
     4501 tcatccggga aatagcgacg gtccggagat aatcatcccc tcccgtcgat cgaattccct
     4561 gcatcgccgt gtttgttgaa atgaagctcc aggggataaa ggaacgaaaa ccattcgttt
     4621 tgtcctgaag ttcgcgaagt ttcaggagat ggacgattct ttcctctttc gtttcaatat
     4681 gcccaaacac catcgtcgtt gttgttttga gacccgcctc atgcgccatg gccatgactt
     4741 caagccatcg ctcggcagaa atctttttcg ggctgatttt ttgacgaacg cgttccacaa
     4801 gaatttccgc gcccccgccc ggcaaagagt tcagacccgc cgctttgaac atccggaaaa
     4861 ggtccggcag ctcaaccccg cttttcttcg acaagatatc catttcaatc gccgaaaacg
     4921 agtgaatctg aatttggggg aacttttcgt gaatggtccg aaccatggcc agatagtcat
     4981 caagctccaa atcaggatgg atccctccct ggatcaaaac ctgcgttcca ccttcgtccg
     5041 cgagctcctg aaccttacgg agaatgacgt ctaaaggaag gacaaaggct tcaggtgatc
     5101 ccggagggca atgaaacgcg caaaaatcac agccaatagt gcaaacattc gtataactga
     5161 tattacgatc cacgacaaac gtaacggccc gtggagaaac ccgcggcgtc aacttttgcg
     5221 ccgccatccc gagatcaaga aggtcacagg aaaagagctc gactccttct tcaagagtca
     5281 atcgttcccc cgccaatcgt tttcgcaata tttgttgatg gctgttcata tcatttttcc
     5341 cgcgcaagtc gcaacgattt cattttaaat ttaactttgc cgttgactgt taaatttttc
     5401 gctactcaaa gaactccagt ggtttcggct caggagcaag gttctcccgg aaagccagat
     5461 cataaaaccg caaaagcccg cgaatcattt cctcatcaag atcataacgc aggttgacga
     5521 agtagccgag catggagaca aactttttct catctggcat gaccccaagg gcattcttca
     5581 aaagccgttc aggttcgcaa agatgacggg accgattttc tttaagaagg gtacaaaatt
     5641 ttcgcaactc cgccccgcat tccgaagcac aatcccgtct caaggtccag agagaaaaca
     5701 caaacggcgt gtccgtccat tccctccaca gcttcccgag atcatacttg taaacccaat
     5761 tctccggctc ataaaaaaga gcgacatccc cgatcatcaa agcggcccga taccgctgaa
     5821 acgcgtcatc aatatcctgg ggaacgctca cccattcgtt tttaaatccc aagcggcgtt
     5881 tcatgagaat cttgagcatg gtcactgaac tcagactctc acgcgacacc gcgatcgttt
     5941 ccccattcaa ttcattcagc ggaactttgg aaaagaggat cacactgcgg acaaaagtat
     6001 ccgaaccaat gccaataccc ggaagcagaa aataatcttt ttgtctcagg agatattcca
     6061 tggaggaaac gggcgccaga tcaatttcct ccgcggccaa agcacgatta agatccgcca
     6121 gacttgcttc catccagctt gtttcaagcc cctgctctcc gcattttttt tcaagctcaa
     6181 aatagaatgg caggcaattg ag
//
LOCUS       DAOSIO010000097         6134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_11587_length_6134_cov_4.510117, whole genome shotgun sequence.
ACCESSION   DAOSIO010000097 DAOSIO010000000
VERSION     DAOSIO010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6134
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11587_length_6134_cov_4.510117"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..749)
                     /gene="thiF"
                     /locus_tag="PK590_04620"
     CDS             complement(132..749)
                     /gene="thiF"
                     /locus_tag="PK590_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HOG23914.1"
                     /translation="MNQSIFDKNLLIKLGPDRFQKITCAKIGIAGAGGLGSNCAVTLV
                     RAGFRNFMIADFDEVDFTNLDRQAYFEDQVGMKKVEALKINLLRISKNLRVDVFDVKL
                     DSSNISQIFSGCDVMVECLDCPQMKSLFVSELLPLGKLLVTASGIGGVGANDEIRVHR
                     IKENLVIVGDLKSDVEKSPALAPRICIAAAKQADVVMEHIMNGRV"
     gene            complement(746..1858)
                     /gene="thiH"
                     /locus_tag="PK590_04625"
     CDS             complement(746..1858)
                     /gene="thiH"
                     /locus_tag="PK590_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HOG23915.1"
                     /translation="MTFYNTLQESEACLRSFDAHEISRGSVIRAVESEARSPERLLTL
                     LSPQASDDLELMARKAHEITLRQFGRVIQLYTPLYLSNFCDNQCSYCGFNARNRVPRK
                     TLTLEEIEREAAYIASTGLKHILILTGGSRAHAPLSYIKSAVRVLKKYFSSISAEIYA
                     LSEGEYAELATEGVDGLTIYQETYHEAVYDRVHKAGPKKDFQFRLEAPERAARGAFRN
                     VNIGALLGLSDWRRESFFLGMHAGYLQDRFPNVEISASVPRIRPHEGDFSGFEQVSDR
                     DLVQMILALRLFLPRLGITLSTRESPWLRENLVPLGVTRMSAGSTTEVGGHTVACHDA
                     EDASQFKIADERNVEEIKAMLVARGYQPVLKDWMRL"
     gene            complement(1891..2670)
                     /locus_tag="PK590_04630"
     CDS             complement(1891..2670)
                     /locus_tag="PK590_04630"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HOG23916.1"
                     /translation="MEDLFRVGGREFQSRLLLGTGKFANYAVMKKAIEESGSDIVTVA
                     LRRVDPGSQADNILDFIPAQCTLMTNTSGARNADEAIRIARLAKAGGAGNWIKIEVVQ
                     DNKYLLPENLETLKAVEVLVKEGFVVLPYMSPDLSMARRMEDAGAAAVMPLGAPIGSN
                     RGIKTRELVEIMVREIKVPVIVDAGIGSPSQACECMEMGCAAVLVNTAVAISENPVQM
                     ASAFRDAVMAGRRAHLARMPEKGGLSGVASSPLTGFLSDEK"
     gene            complement(2671..2871)
                     /gene="thiS"
                     /locus_tag="PK590_04635"
     CDS             complement(2671..2871)
                     /gene="thiS"
                     /locus_tag="PK590_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOG23917.1"
                     /translation="MLIRVNGKEEQIDQPLSLKELVLRKGLQLDRIVIEHNGVILPRS
                     SWGSVRLTEKDALEIVSFVGGG"
     gene            complement(3138..4904)
                     /locus_tag="PK590_04640"
     CDS             complement(3138..4904)
                     /locus_tag="PK590_04640"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HOG23918.1"
                     /translation="MIKNPPSGLVLKPESFFRRKQRQIHFYLSTTFRLHFIHFLVCFA
                     IIAGHMMMMDSRLLQRLEYVFLDLFYRQRPPITQHAAITHIDMAEDSLHVIGRWPWPR
                     HNHAALTHILHEWGARAVVFDVIFSEPSTSFDDESLTESLKESGLVYLPSVLETFNNQ
                     KEWIHSLPEFKMLSKGTGHVNVVPDSDGTLRRIVPYLEYNNEIHPYIALRVAYDALGK
                     PMPHPKKLPFPTDSDGNMMINWAGKWRDTFDHISFIDLIKSYAALKSGATPLITPDRI
                     KGKICVIGLTAVGLTDIKANPLEEVYPAVGVQTNILNSILTNQFVYPASRKANLICLL
                     LVGFFASLIFIFSKQILSLIMGLAWGFVWILFAFVMFSTRGIWFFALNPLLLIFSLFV
                     FFVVFTLTIGNRERERLFTLATRDGLTGLYVIRHFRMLLNDAVIEAHKKKTELSVILF
                     DIDFFKKVNDTFGHAAGDAVLKRLAQLMREVFPEDTKKKKNPYSLGRYGGEEFIILIR
                     NCSLLDAAFNFGEKLRKRVEQEVFVFEGKEIPFTLSLGVATLGPTETVPDLMVHRADE
                     ALYRAKTQGRNRTCIEKDEGAA"
     gene            complement(4901..5623)
                     /gene="nth"
                     /locus_tag="PK590_04645"
     CDS             complement(4901..5623)
                     /gene="nth"
                     /locus_tag="PK590_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOG23919.1"
                     /translation="MKETLEKKKRRASSIVRRLRKTYPDVRCALHFSNPWELLVATIL
                     SAQCTDKRVNKVTPVLFKKYPKISDYAGARVGQLESIIRSTGFYRNKAKNIIRAARVI
                     LAKFEGEVPRTMESLVTIPGVGRKTANVVLGNAFGIPGISVDTHMIRLNRLLGFTRHT
                     DPVKIEFDLMKIVPRRDWTLFSSLVIRHGRTRCPARRPDCPHCEIVRQCASAGKDSAR
                     ISETYGQGKARDNFRKGSRTLI"
     gene            complement(5739..>6134)
                     /locus_tag="PK590_04650"
     CDS             complement(5739..>6134)
                     /locus_tag="PK590_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23920.1"
                     /translation="FHSEGVIMTKEHETENEDMELEEEADVLGLLKRIQQQLGFLEKK
                     IDTLLAKSSGSSTERPRFSEHRKPFRRSFNHGPREDGFRSRDQGGGFNQGRRDDRGNS
                     FERPRGNSGRSFHRQDGGFRGKKRFGGSR"
BASE COUNT         1668 a         1649 c         1343 g         1474 t
ORIGIN      
        1 acattacgag tcatccacaa ttctcatggt aaggttttaa tatgaggcaa cgggccggtt
       61 catttattaa tctttatggt atcgatcttt tagagctctt cgcaccaaac cgggatggcc
      121 gagcgaagaa atcagaccct gccgttcata atatgttcca tcaccacatc tgcctgtttg
      181 gcagccgcga tacaaatccg gggggctaac gcgggtgact tttcgacatc cgacttcaaa
      241 tcacccacga tcacaagatt ttccttgatc cggtgaacgc gaatttcatc atttgccccc
      301 acgcctccaa ttcccgaagc cgtcacgagc aacttcccga gcggtaaaag ctcggaaaca
      361 aaaagacttt tcatctgggg acagtccaga cattcaacca ttacgtcaca acccgaaaaa
      421 atctggctga tattggaaga atccagtttg acgtcgaaga cgtccacccg caaatttttc
      481 gagattctga gaagattgat tttaagcgcc tcaacttttt tcattccaac ctgatcttca
      541 aaataagcct gccgatccaa gttagtaaaa tcaacttcat caaaatcagc tatcataaaa
      601 ttacggaatc cggccctcac cagcgtcacg gcgcaattcg agcccaatcc cccggcgcca
      661 gcgatgccga tcttcgcgca agtaatcttc tgaaaacgat ccgggccaag cttgatcaga
      721 agatttttat caaaaatact ctgattcata accgcatcca gtcttttaga accggctgat
      781 agcccctggc cacaagcatc gctttgattt cctcgacatt tcgctcatcc gcgatcttaa
      841 attgcgaagc gtcttctgca tcgtgacatg cgaccgtgtg cccgcccact tctgtggtcg
      901 accccgccga catgcgcgtc actccgagcg ggaccagatt ttcgcgcaac cagggggatt
      961 cgcgagtcga aagagttatc ccaagtcggg gcaaaaacag tctcaaggcc agtatcattt
     1021 gaacaaggtc acggtcggac acttgctcaa acccgctaaa atcaccttca tgaggtcgaa
     1081 tgcgcgggac agacgcgcta atttccacgt tcgggaaacg gtcctgtaga tacccggcat
     1141 gcattccaag aaaaaaagat tcccgtctcc agtcacttaa accgagcaaa gccccgatat
     1201 taacattccg gaacgcgccg cgggccgccc gctccggcgc ttcaagtcga aattgaaaat
     1261 ctttcttagg ccccgcctta tgcacgcgat cgtaaacggc ttcatgataa gtttcctgat
     1321 agatcgtaag accgtccaca ccttcggtcg cgagttcagc atactctcct tcactcagag
     1381 catagatttc cgctgaaata gaactaaaat attttttaag aactctaacc gcagacttga
     1441 tataggaaag cggcgcatga gcacgggagc ctcccgtcaa aatgagaata tgcttcagcc
     1501 ccgtagacgc aatataagcc gcttcacgct caatttcttc aagggtcagg gtttttcgcg
     1561 gaacacgatt tcgggcgtta aaaccacaat aagaacactg attgtcgcaa aaattggaca
     1621 gataaagagg cgtgtaaagc tgaataaccc tgccaaattg tcgcaacgta atttcgtggg
     1681 ctttgcgggc catcaactcg agatcatctg atgcttgggg agaaagaagg gtcagaagcc
     1741 tttccggcga acgcgcttcc gattcaaccg cccgaatgac agatcccctg gaaatttcgt
     1801 gagcatcaaa actccggaga caggcctcgc tttcttgaag agtgttataa aaagtcataa
     1861 attctcacaa ttaaagataa agattcctgc ttatttttcg tcactcaaaa aaccggtaag
     1921 aggcgacgaa gcaactcctg acaatccgcc cttttccggc atcctggcca aatgggcacg
     1981 acgcccggcc atcacggcat ctctaaaagc cgaagccatt tgcaccggat tttcagaaat
     2041 ggcaaccgca gtattcacta aaactgccgc gcatcccatc tccatacatt cacatgcctg
     2101 cgacggactg ccaatccccg catccacaat aacgggtact ttaatctcac gaaccatgat
     2161 ctcgacaagc tcacgggttt tgataccgcg gttacttccg atcggagcgc ctagtggcat
     2221 cacggcagcc gcgccagcat cttccatgcg ccgcgccatg gaaagatccg gactcatata
     2281 agggagaacc acgaaccctt ccttgacaag aacttcaacc gccttcagag tttcaagatt
     2341 ttccggcaaa agatatttat tatcctgcac tacttcaatt ttaatccagt tccccgctcc
     2401 cccagccttg gcaagacggg caattctgat cgcctcgtca gcattacggg ctccggaagt
     2461 gttggtcata agagtacatt gggcgggtat aaaatccaaa atattgtccg cctgagaacc
     2521 aggatcaacg cgccgaaggg ccacggtaac aatatccgaa cccgattctt cgatcgcctt
     2581 tttcataacg gcgtagttcg cgaattttcc ggttccaagc aaaagacgac tctgaaattc
     2641 acgtcccccc actctaaaca gatcttccat ttagcctcct ccgacaaaac tgacaatttc
     2701 aagtgcatcc ttctcggtaa gacgtaccga accccaggat gagcgcggca aaatcacccc
     2761 attatgttct atcacaatcc ggtcaagttg gaggcccttc ctcaaaacaa gctccttgag
     2821 actcagaggt tgatcaatct gctcttcctt gccattaact ctgataagca tgagtgcgtt
     2881 ccttggcgtg tcatcacggg gaaacttata aacctgaaag acgggtcgat tatagcggga
     2941 atggccctgc ggcacaacca gcttggaaag tagccgcgga cttatccagg aaacaggtct
     3001 gaaatgactt cccaaaactg ctcggcgtgg gatttgtgaa tttctccgga aactctggat
     3061 cccctgacct tacgtctgat tttccgtccc ggaaagccgt gaaagagaag acgagtttac
     3121 agtttacgaa accgattcta cgctgcccct tcatcttttt caatgcaagt tcggttacgc
     3181 ccttgtgttt tagctctgta caacgcttcg tcagcacgat gaaccattaa atcgggaacc
     3241 gtttcagtcg gccccaaggt cgcaactccc agactaagcg taaaagggat ttccttccct
     3301 tcgaatacaa agacctcttg ctcaacccgc ttgcgaagct tctccccaaa attaaaagcg
     3361 gcatcaagca aggaacagtt tctgattaaa atgataaact cctccccgcc gtaacgcccg
     3421 agagaataag ggttcttctt ttttttggta tcctccggga acacttctct catcagttgg
     3481 gcaagtcttt tcaatacagc gtccccggca gcgtgaccaa aagtatcatt aacttttttg
     3541 aagaaatcaa tgtcgaacag gatcacagac aactctgtct ttttcttgtg ggcttcgatg
     3601 accgcgtcat taagtaacat ccggaaatgg cgaataacat aaagaccggt aagaccgtcg
     3661 cgggtcgcga gcgtgaacag acgctcccgc tccctatttc cgatcgttaa tgtaaaaaca
     3721 acgaaaaaaa caaataggga aaaaatcaac aataacgggt tcagcgcgaa aaaccagatc
     3781 ccccgggttg aaaacatgac aaacgcgaaa agaatccaga caaaccccca agccagcccc
     3841 atgatcagtg aaagaatctg cttggaaaaa ataaaaatca gcgaagcaaa aaaaccaacc
     3901 agcaaaaggc agatcaggtt cgcctttctg gaagctggat aaacaaactg gttagtaagg
     3961 atactgttaa ggatattggt ctgaacaccc acggcggggt aaacctcttc gagaggattc
     4021 gctttgatat ccgtcagtcc gaccgcggtc agcccgatca cgcaaatttt tcccttgatg
     4081 cggtcggggg taatgagcgg tgtcgcaccc gactttaaag cggcataact tttgatgaga
     4141 tcaataaacg agatatggtc aaacgtatcc ctccatttac cggcccagtt aatcatcatg
     4201 tttccatccg aatccgtcgg aaaaggcaac tttttcggat gaggcatcgg cttcccaagc
     4261 gcgtcatagg ccacccgcaa ggcaatatag ggatggattt cattattgta ttccagataa
     4321 ggaacaattc gtcgaagcgt cccgtcagaa tctggcacga cattcacatg ccccgtccct
     4381 ttactcaaca tcttgaactc gggcaaagaa tggatccact ccttttggtt gttgaaggtt
     4441 tccaaaacgg aaggtaaata aaccaggcct gattccttca gagattcggt cagagattca
     4501 tcatcaaagc tggtgcttgg ctcactgaat atgacgtcaa aaacaacggc ccgggctccc
     4561 cactcgtgga gaatatgggt cagcgccgcg tggttgtgac gaggccacgg ccatcgcccg
     4621 atcacatgga gactgtcttc agccatatca atatgggtga tcgcggcatg ctgcgtgatc
     4681 ggtgggcgtt gccggtaaaa caagtcaaga aacacatatt ccagccgctg gagcaaccgg
     4741 gagtccatca tcatcatgtg ccccgcaatg atcgcgaagc aaaccaggaa atgaataaag
     4801 tgaagccgga aagtggtgct taggtaaaaa tgtatttgcc gctgtttgcg acggaaaaaa
     4861 ctttccggtt tcaggaccag ccccgaagga ggatttttta tcatattaac gttcttgatc
     4921 cttttctaaa attgtctctc gcttttccct gcccataagt ttcggaaatc ctggctgaat
     4981 ctttacccgc ggaggcacat tgccgaacaa tctcacaatg aggacaatcc ggccggcgcg
     5041 cgggacaccg cgtccgtcca tgacgaatga ccagactgga gaaaagtgtc cagtctctcc
     5101 ggggaacaat cttcatcaaa tcgaattcga tttttaccgg atctgtgtgg cgggtgaatc
     5161 ccagcaaacg gttcagccgg atcatgtgcg tatccaccga aattcccggg atcccaaaag
     5221 catttccgag caccacattt gccgtttttc ttcccacgcc gggaatcgtc acaagcgatt
     5281 ccatcgtcct cgggacctcc ccttcaaatt tcgccaaaat tactcgggcg gctcttataa
     5341 tattttttgc cttatttcta taaaatccgg tggagcggat gatggattca agttgcccca
     5401 cccgtgctcc ggcataatct gaaatcttgg gatatttttt aaacagaacc ggggtaactt
     5461 tattcactcg cttgtcggta cactgggccg aaaggatggt cgccaccaaa agctcccagg
     5521 ggttcgaaaa gtgaagcgcg caccgaacgt cagggtatgt tttcctgagc cgccggacaa
     5581 tgctggaggc ccgtcttttt ttcttttcta aggtctcttt catgggagaa aatattagca
     5641 aatcctgtga aaggtatggg gtacaaccca aaagaagtaa tcaatcaact cagatcgttt
     5701 ctgaattgtc acaatccctg ggccttacaa aaattcgcct aacgggaacc gccaaatcgt
     5761 ttcttgcccc gaaaaccacc atcctgtcga tgaaaagatc ttcccgagtt tcctctcgga
     5821 cgttcgaaag aatttccccg gtcatcacga cgtccctggt tgaatccccc accctgatca
     5881 cgcgatcgaa aaccgtcctc gcgcgggcca tgattgaaag aacggcgaaa cggcttccgg
     5941 tgttccgaaa aacgcggtct ttcggtcgag gagccagagg acttggccag taacgtgtcg
     6001 atcttttttt ccaaaaaacc cagttgttgc tggattcttt tcagtaaccc taaaacatcc
     6061 gcttcttcct ccagttccat gtcttcattt tcggtttcgt gttctttggt catgataact
     6121 ccttcagagt ggaa
//
LOCUS       DAOSIO010000098         6124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_11613_length_6124_cov_3.687098, whole genome shotgun sequence.
ACCESSION   DAOSIO010000098 DAOSIO010000000
VERSION     DAOSIO010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11613_length_6124_cov_3.687098"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..201)
                     /locus_tag="PK590_04655"
     tRNA            complement(128..201)
                     /locus_tag="PK590_04655"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(165..167),aa:Val,seq:tac)
     gene            complement(252..1301)
                     /locus_tag="PK590_04660"
     CDS             complement(252..1301)
                     /locus_tag="PK590_04660"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HOG23921.1"
                     /translation="MSKTRFKKYGANTGDPRIDTLIRQLVRDIGPGENADLVEELISS
                     AIGVYRDGLERGDMKIINTAVKELRRSLHVFEPYRAIRKVAVFGSARTPRTDPNYQIT
                     LKFSKAIVRKQWMVITGAASGIMEAGNAGAGSKGSFGMNIRLPFEQEANSVILKDPKL
                     VTFKYFFTRKLMFLRESHATILCPGGYGTHDEGFETLTLVQTGKTEPRPIVCLDAPGS
                     TYWQDFKEFLEKQLVKNEMIHPDDIDLMFFTQDADEAVDHILNFYHRYHSMRYIRDLL
                     VLRIEKALTESQLDDLNRRFASIIRKGKIQQSLKPFEEEKNAPHTFHLPRLSFYFKKD
                     CHHVLKKMIDAINSF"
     gene            complement(1301..2002)
                     /locus_tag="PK590_04665"
     CDS             complement(1301..2002)
                     /locus_tag="PK590_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOG23922.1"
                     /translation="MKQKDYYQVLGVSESATADEMKKVYRKLAVKYHPDKNPGNKEAE
                     AKFKEISEAYYVLSDEKRRKQYDQMRKFGGSFQGNYAGAQGFDFEELLRQFSGRGART
                     QGRYSAFSDIFSDLFGGGMGNGGGSYQFYSAGPQSGSHPRHYQSASSSYDDGYVGQTQ
                     DIESDVTVGLKISKKRAKEGGEVTFKTHEGKMISVKIPAGVREGQKLRLARQGRECPA
                     CHHPGDLILKIKVGE"
     gene            complement(2104..4224)
                     /locus_tag="PK590_04670"
     CDS             complement(2104..4224)
                     /locus_tag="PK590_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOG23923.1"
                     /translation="MTDILSALNSTQREAVTWPKETPLLILAGAGSGKTRILTHRIAH
                     LISQGVPGFHILGVTFTNKAAGEMRSRVEKLTRQQVWISTFHSTCLRILRQDSAAIGM
                     DPKFIIYDEYDQLQIIKECLRDLNLDEKKIHPKGVREEIQRAKDYLLTPYQFAEKTMD
                     LYQDSVAKIYLLYEEKMKRLKACDFGDLIMKTVVLFDRAPEVLKSWQNRFQHILIDEY
                     QDTNHAQYRFVKQLASTHKRITVVGDPDQSIYAWRGADIKNILNFEDDYSGCGMIRME
                     QNYRSTPVILEAANQLIRNNQMRKPKDLWTEKEGGEKIELYDAEDEADEAMHVVSNVM
                     RRRAQGTRLGEQVVFYRIHAQSRVLEDHLRRANIPYKIVGGVRFYDRKEIKDVIAYLR
                     LIAYPSDELSLKRVINVPARGIGKKAMEILEREVVRLRNLEKETTATSSPTGANLDSR
                     AAGGEIQPGLATWDSVLSNVVKIEGLSPRTKKNLADFYDLIRNLRRKASELSVRELLE
                     QVLEGSRYVAELQAEHTAEAETRIENIQEFFSVIEDFEEQGSSLLDDSGEGVTLTPLE
                     SFLESIELVTDLDTWDSEKDVLTLMTLHTAKGLEFTVVHMVGLEEGIFPHVNSYLGSA
                     DEIEEDRRLCYVGITRARESLHMSYAAARRLYGATHHNLPSRFLTEIPSHLFKTARRN
                     TRYENDRNEWIDSGIEDDPVIDFD"
     gene            complement(4295..4678)
                     /locus_tag="PK590_04675"
     CDS             complement(4295..4678)
                     /locus_tag="PK590_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23924.1"
                     /translation="MAEERNEYLKKIELLVDQNPDYKLEAYTFVLAVLHDTVSRLSKP
                     RHVAGKELLEGIRQYAIRQFGPMALTVIRYWGIHETLDFGKIVFRLVESKLLSKQPQD
                     QLEDFMDGYDFEEAFGKYKIDDETL"
     gene            complement(4726..5400)
                     /locus_tag="PK590_04680"
     CDS             complement(4726..5400)
                     /locus_tag="PK590_04680"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23925.1"
                     /translation="MSWKRLPTLSAPMSLQMALDEILFDRFKQNLRSGAVLRFYHSSS
                     PWISVGYSFKTKESLRSSELIRTHSEIPLVSRMTGGGCVLHGEDLIFSLIASCAEIPR
                     LQSVKESYAAIHHVVKMGIGRFGMEARFYEEDEELPKGADCFSYPVARDLSLGNRKVA
                     GGAQKRSEGVLLHHESIVLPRYVNSGDLAHAIQAGFEKVFNVSVELMDFDPEIYFEAE
                     RKCSTL"
     gene            complement(5492..>6124)
                     /locus_tag="PK590_04685"
     CDS             complement(5492..>6124)
                     /locus_tag="PK590_04685"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOG23926.1"
                     /translation="PVGVKKHLIPFLPEPLVEKTKDHYAWAQHSEKSIGRMKSFHGNV
                     GVLLRAYAYLKVIGKEGLMRVSEGAILNANYLREKLKDVFQLAVNEPCLHEFVLTLKK
                     AAKKGIHAGDVGKRLLDFGFHAPTVHFPLVVAEALMIEPTETESRETLDLFVEAMKRI
                     AFEIENEPDKVKNAPFTLPVRRPNDVLAARRPILTYRDLCRAKNTTEIAA"
BASE COUNT         1537 a         1695 c         1302 g         1590 t
ORIGIN      
        1 ctccggactg tcgatccgga ggttgcgggt tcgagccccg tcgctcccgc catttttttt
       61 ggcgcaatct cgcgacgctt ccgcgtcatg ttccccgccg gttcaaaaaa tcgagaccgt
      121 tcgccattgg gcaatagagg attcgaacct ctgaccctct ccgtgtaaag gagatgctct
      181 aaccaactga gctaattgcc caacggcgaa acgctgacat cacttcttaa agaaacgtta
      241 tcccccaacg tttaaaatga attgatcgcg tcgatcatct tttttaagac atggtgacag
      301 tctttcttga aataaaacga cagccggggc agatgaaacg tgtgcggagc gtttttttcc
      361 tcttcaaagg gtttcaggga ctgctggatc ttgcctttcc ggataatgga agcgaaccgc
      421 cggttaagat catctaattg tgattcggta agagcttttt caattctgag caccaaaaga
      481 tcccgaatgt agcgcatgga gtgatatcgg tggtaaaaat tcaaaatgtg atcgaccgcc
      541 tcgtcagcat cctgcgtgaa aaacatcaga tcaatgtcat cggggtgaat catctcgttc
      601 ttcaccaact gcttttccag aaactcttta aaatcttgcc agtaagtcga acctggcgcg
      661 tccaggcaaa cgattggccg cggttcagtc ttgccggtct gcaccagcgt taacgtctca
      721 aaaccttcat cgtgagttcc gtaccccccg gggcagagaa tcgtggcatg agattctctg
      781 agaaacatga gctttcgcgt aaaaaaatat ttaaacgtaa caagcttggg gtctttcaat
      841 atcacagaat tcgcttcttg ctcgaacgga aggcggatat tcattccgaa actccccttg
      901 ctccccgctc ccgcgtttcc cgcctccata attccggaag cggcccccgt aatcaccatc
      961 cattgtttcc gaactatggc tttggaaaac ttgagagtga tctggtaatt gggatccgtt
     1021 ctgggcgttc tcgccgaacc aaaaacagcc acttttcgta tcgcccgata aggttcaaac
     1081 acgtgaagac ttcgacgcaa ttccttaacg gccgtattaa tgatcttcat gtcaccgcgc
     1141 tccaacccgt cccggtaaac accaatcgcc gaggagatca gctcctccac caggtcagca
     1201 ttctccccag gaccaatatc acgcacaagt tggcggatca gcgtgtcaat ccgcggatct
     1261 cccgtattcg ctccgtattt tttaaaacgt gttttcgaca ttattccccc actttaattt
     1321 tcaggattaa atcccccgga tgatggcagg ccggacactc ccgaccctgg cgggcgaggc
     1381 ggagtttctg accttccctg actcccgcgg gaattttaac cgatatcatt tttccttcat
     1441 gagttttgaa agtcacttcc ccgccttcct tggctctttt ttttgagatc ttgaggccaa
     1501 cggttacatc cgattcaata tcctgggtct gtccaacata tccatcatca tagctggaag
     1561 aagccgattg ataatgccgg ggatgcgaac cactttgggg ccccgccgaa taaaactgat
     1621 aggaaccccc gccatttccc atcccccctc caaaaagatc cgagaaaata tcagaaaaag
     1681 ccgagtaccg gccctgggtt ctcgcgcccc ttccggaaaa ctgccggaga agctcctcaa
     1741 aatcaaaccc ctgcgcgccc gcataatttc cttgaaaact cccgccaaac ttccgcatct
     1801 ggtcgtactg tttacgccgt ttttcatcac ttaatacata gtaggcttcg gaaatttcct
     1861 taaatttggc ttccgcctct ttattgcccg gatttttatc gggatgatat ttaaccgcca
     1921 gttttcgata cactttcttc atctcgtctg cggtcgcaga ttccgaaact ccgagcacct
     1981 gataatagtc tttctgtttc atctatctca ccttgggctg atccaaccgc ggggacattc
     2041 ggtcagttag ggcgctatct tatacaaatt gtcatcactt ttccatcaca actcattcca
     2101 ctctcaatca aaatcaatga caggatcatc ttcaatccca gaatcgatcc attcattccg
     2161 atcattctca taacgcgtat ttcgtcgggc cgttttgaag agatgggaag ggatctccgt
     2221 caaaaatctc gaaggaagat tatgatgtgt ggctccataa agccggcggg ccgccgcata
     2281 ggacatgtga agggactcac gggcgcgcgt aatcccaacg tagcaaagcc gccgatcttc
     2341 ttcaatttca tccgctgaac caagatagga gttcacatgc ggaaaaatcc cttcttcgag
     2401 acccaccata tgtaccaccg tgaactcgag acccttcgcc gtatgaagcg tcatcaatgt
     2461 caacacgtcc ttctccgaat cccacgtatc aaggtcggtt acaagttcga tcgactcaag
     2521 aaatgactcc agaggcgtta atgtaactcc ctccccggaa tcatccaaca aagaacttcc
     2581 ctgttcctca aaatcctcaa tgacactgaa aaattcctgg atattttcaa tcctcgtttc
     2641 ggcttccgct gtgtgttcgg cctgaagttc cgcgacgtaa cgggatcctt ccaacacctg
     2701 ctccagaagt tcccttaccg aaagctcact tgcttttctt ctaagatttc tgattaaatc
     2761 ataaaaatcc gcaaggtttt ttttagttcg cggcgaaagt ccttcgatct tcacaacatt
     2821 tgacagaaca gaatcccaag tagccaatcc gggttgaatc tcgcccccag cggcccgaga
     2881 atccagattc gcgcccgtcg gcgaggacgt agctgtcgtc tccttttcaa gatttcgaag
     2941 ccggaccacc tctcgctcca atatttccat agcttttttc ccgatccctc gcgccggcac
     3001 attaatcacc cgtttcaagc taagttcatc cgagggatac gcgatcaacc gtaagtaggc
     3061 aatcacgtcc ttgatctctt tacgatcata gaaccgtacc ccaccgacaa tcttatatgg
     3121 gatattcgcc cgacgaagat ggtcttccaa aactcgcgac tgggcatgga ttcggtaaaa
     3181 aacaacctgt tctccaagac gcgtaccctg ggcacggcgg cgcatcacat tcgacaccac
     3241 atgcatggcc tcatcggctt catcttccgc gtcataaagc tcgatctttt cacctccttc
     3301 tttttcagtc cagaggtctt taggttttct catctggttg ttccggatca gctggttggc
     3361 cgcctcaaga atgacgggcg tcgatcgata gttttgttcc attctgatca tgccgcaacc
     3421 ggaataatca tcttcaaaat tgagaatatt cttgatgtcc gcgccacgcc acgcatagat
     3481 cgactggtcc ggatctccca ccaccgtaat tcttttatgc gtcgaagcga gctgtttcac
     3541 aaaccggtat tgggcgtggt tcgtatcctg atactcatcg atcagaatat gctgaaatcg
     3601 gttttgccat gactttaaca cctccggcgc gcggtcaaag agcacgacgg ttttcataat
     3661 caaatctcca aaatcgcacg ccttgagcct cttcatcttc tcctcataca acaaatatat
     3721 cttggccact gaatcttgat aaagatccat ggtcttctct gcgaactggt acggtgtcag
     3781 gaggtaatct ttcgcccttt gaatttcctc acgaacccct ttgggatgaa tttttttttc
     3841 atcaaggttg agatccctga gacattcttt aataatctga agctggtcgt attcatcata
     3901 aataataaat tttggatcca tgccgatggc ggcggaatcc tggcgcagta tcctgagaca
     3961 tgtcgaatga aacgtgctga tccagacttg ctgtcgcgtc agtttttcaa cacgtgaacg
     4021 catttcacca gccgctttgt tggtgaaggt cacgcccaga atatggaacc cgggaactcc
     4081 ctgtgaaatg agatgcgcga tccggtgggt aagaatccgg gtcttcccgc ttccagcacc
     4141 cgccaaaatc aaaagcggag tttctttggg ccaggtgacg gcttcgcgtt gcgtcgaatt
     4201 gagcgccgag agaatatcag tcatagtgtt tcgtgaaaac cggcagggtc aggcataaag
     4261 tgttttgaaa actgtccttg agttcatgat ctcatcacaa agtttcatcg tctattttat
     4321 atttcccaaa agcttcctca aaatcgtacc catccataaa atcttccagt tgatcctggg
     4381 gctgtttgct aagcagtttc gattcaacga gacgaaaaac gatcttcccg aaatcaagcg
     4441 tctcatgaat cccccagtac cgaatcaccg tcagggccat tgggccaaac tgtcgaatgg
     4501 cgtattgacg gataccttca agaagctcct tcccggcaac atggcggggc tttgacaatc
     4561 tcgacactgt atcatggagc accgcaagca caaacgtata agcctcaagc ttatagtccg
     4621 ggttctggtc cacaagcaat tctatctttt tcaaatattc atttcgttct tccgccatgg
     4681 cacgacactc ttaatccccc gaaaatcagt cgggtatctc gaagcctata acgttgagca
     4741 tttcctttcc gcttcaaaat aaatctccgg atcaaaatcc ataagctcaa cactaacgtt
     4801 aaaaactttt tcaaatccgg cctggatcgc gtgcgccaga tcaccactat tcacgtaccg
     4861 agggaggaca atggactcgt ggtggagaag aacaccttcc gacctttttt gagcccctcc
     4921 cgccaccttc cggtttccca gcgaaagatc tctggcaacc ggatagctga aacagtcggc
     4981 tcctttcgga agttcctcgt cttcctcata aaaccgagcc tccataccga aacgtccgat
     5041 ccccattttt accacatggt ggatagcggc ataactttct ttcacggatt gaagccgtgg
     5101 aatctccgcg caggaggcaa tcaaactaaa aataagatct tctccgtgca acacacatcc
     5161 tccccccgtc atccggctga ccagaggaat ttccgaatgg gtccttatga gttcggaaga
     5221 ccgcaaactc tccttcgttt taaacgagta acccacgcta atccagggag acgatgaatg
     5281 ataaaaacgc agtaccgctc cagaacggag attttgctta aaccggtcaa acaagatttc
     5341 atctagcgcc atctgcagtg acatcggcgc gctgagggtt ggcaaccgct tccaggacat
     5401 agggtgctct agtggtcaga ggccaacgaa gccacaactc cccgcgaggc agacaagcaa
     5461 aaaacaggag cctcgggatc cccatgtcga ctcatgcggc aatttccgta gtgttttttg
     5521 cccgacaaag atcacgataa gtgagaatcg gtcttcgcgc ggcaagtacg tcattggggc
     5581 ggcgaaccgg caaggtgaaa ggagcatttt ttaccttgtc cggctcgttt tcaatttcga
     5641 aagcgattcg tttcatcgcc tcaacaaaaa gatccagcgt ttcccggctt tccgtctcgg
     5701 tcggttcaat catcagcgct tcagccacca ccaacgggaa atgaaccgtg ggggcatgaa
     5761 acccgaaatc aagcaacctc ttgccgacat cgccggcatg aatccccttc ttggcggcct
     5821 ttttgagagt gagaacaaat tcatgaaggc aaggctcatt gacagccagc tgaaaaacat
     5881 cttttaattt ttctcgtaaa taattcgcgt tcaaaatggc gccttcgctc accctcatca
     5941 ggccctcttt gccaataacc ttcagatagg cgtaggcgcg caaaagaacc cccacgttcc
     6001 cgtgaaaaga tttcatgcga ccgatactct tttccgaatg ctgggcccag gcatagtgat
     6061 cttttgtttt ttcaacgagc ggctcgggca aaaacggtat taaatgtttt ttgaccccaa
     6121 cagg
//
LOCUS       DAOSIO010000099         6024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_11875_length_6024_cov_4.118445, whole genome shotgun sequence.
ACCESSION   DAOSIO010000099 DAOSIO010000000
VERSION     DAOSIO010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11875_length_6024_cov_4.118445"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(386..1567)
                     /locus_tag="PK590_04690"
     CDS             complement(386..1567)
                     /locus_tag="PK590_04690"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HOG23927.1"
                     /translation="MKQPIPLNEHAPLLNKPVKPAELSGVENLIRQMLKKHKTPFMII
                     RKSVLEKQLSRFQKAFPQVTPYYAIKANPHPRIVKFFAKLGTCFDVASAAEMKLVLKQ
                     GAKPRHIIFANTIKSKEDLVTARKRRVRLMTFDNEAELYKIAENYPGAHVILRIRVAN
                     RGSVVQLSLKFGADPDQGFYLLRKAKGLGLVPKGVSFHVGSQSTNVENYLQALELTAG
                     IFKESKESGIPLSIVDIGGGFPIQHFDNEIGINFERMAGQIRRQMRELFPKSTQFMAE
                     PGRFFVGPAGVLVTQVVGKAFRDHKNFYYLNDGVYQDFSGMMFDHCKYEFKTLRRGQK
                     YMSVFAGPTCDSLDILSSNEEVPELFVGDVVYVKNIGAYSSASAVPNFNGFEPARVIL
                     V"
     gene            complement(1574..1795)
                     /locus_tag="PK590_04695"
     CDS             complement(1574..1795)
                     /locus_tag="PK590_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23928.1"
                     /translation="MIQNPREGFLLPDDIDHEAILKFATPYVAPCVSIPSDWTPLKNL
                     NHKYLKFKAKAPRESDLWQFNSFLVDERV"
     gene            complement(1792..3084)
                     /locus_tag="PK590_04700"
     CDS             complement(1792..3084)
                     /locus_tag="PK590_04700"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HOG23929.1"
                     /translation="METLFRYPKASARIPKTRVPFGGKVLIIGCGGVSQCAVPLILRH
                     FDMPAKKITIMDFVDNRTRVKDALKKGVRYVFDRVTEANYKTLLARYVGSGDMIIDLG
                     WNIGCWEILDWCRTNQVLYVNTSVEEWDPYRDDKRTDPTKYTLYARQMILRKQIARKW
                     GSEKGSTAIVDHGANPGLVSHFTKKALIDIARKIIREKPRDSRVKNLERFIAEKNFAK
                     LAQITGVKVIHISERDTQVTDVPKRVNEFVNTWSIEGFYEEGVAPAEMGWGTHERKLP
                     KDGYAHKTGPKNQICLGTLGMNTFVRSWVPCGEINGMVIRHGEAFSISDRLTVWKNGK
                     AVYRPTVHYAYCPSDSALLSLHELRMRQYKMQEKQRILSDDIIDGRDELGVLLMGHDF
                     KSWCAVRSLISTRPGNWFPTSRRRPCRSRHQLWRPRSG"
     gene            complement(3325..4605)
                     /gene="lysA"
                     /locus_tag="PK590_04705"
     CDS             complement(3325..4605)
                     /gene="lysA"
                     /locus_tag="PK590_04705"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOG23930.1"
                     /translation="MLRGEFQGETRMHDFYYDRKGDLCCEKVPLKKILKKTGTPAYIY
                     SSRTISEHLAKIQKAFRSVRPLICYSVKANSNLAVLKTLVKNGAGLDIVSGGELFRAR
                     KVQCPGRKIVYAGVGKTDEEIREAIRARILLFNVESLPELERIHQIAKAMRKQVNVSL
                     RVNPNVDPHTHAYITTGRAETKFGIDFDRARSIYLESKKLTHATLCGLHVHIGSQIET
                     GEPFVEAFRKILEFVDDLELAGIKIKFLNLGGGLGVIYSDENPQTADEFAKKILPLFK
                     KRKYQIIFEPGRFIVANAGVLAAKVLYVKETAVKKFLITDAGMNDLIRPAFYNAYHGI
                     WPIRKQEHVARWVYDVVGPICESGDFFAKDRELPEMRSGDFLAVMTSGAYGFTMSSNY
                     NSRRRACEVLVKGASFRVVRQRETYQDLVRGEKI"
     gene            complement(4641..>6024)
                     /gene="argH"
                     /locus_tag="PK590_04710"
     CDS             complement(4641..>6024)
                     /gene="argH"
                     /locus_tag="PK590_04710"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOG23931.1"
                     /translation="IDVTKKLWGGRFKKGMHPALQCFSYSLKTDKELFESEVLVNEAW
                     AKMLARTGIITEKESRAIQKGLAAVAKMLEGQDLSLYADRYEDIHTLMQSELEKKVGA
                     VAKKLHTGRSRNDLVVTSTRIYLRGKVLVIQYEIQRLQKSLMTAARRAHDLIVPGMTH
                     LRKAQPVLLAHHLLAYVEMLEEDRLRLCDLMKRVNVMPIGAAALAGSALPIDQKFLAK
                     ELGFEKIAANSMAAVSDRGFLAEFLADLSILWMHLSRLAEDFILWNSEAFGYVELDDS
                     FATGSSLMPQKKNPDVFELVRGRSGVVFGHLQAMLTIQKGLALSYNRDLQEDKPGVFD
                     AIHKTTIALEVLSLTIDSLKWNKKNILDSLEDDTLFATDILEYLVRKKVPFSESHAIV
                     GKAVRYAADSGKKLREFSLNDWKKFSARIGAEVFDLFDAAASVKGKKTIGSTHPDQVE
                     KQLKRWERCL"
BASE COUNT         1484 a         1669 c         1346 g         1525 t
ORIGIN      
        1 aggtttgctt acaaattcgg cctttttttc actgccttaa gtgggataca ccccatcaac
       61 ggatcaacga cctctcggga tcggcaagaa ttcatttgtc cgtctttgag gcgggagatt
      121 ttctttacca attcagaact gtaaagaagg gaataaatcg catcctgctt gtcacagttt
      181 tttatcagac gctaaagaca gggatatcaa atgtcgccac aagatccggt ccctttgctt
      241 tcaagcggaa agaaagctcc cacacgatat caccgtctcc caaacgttcc atcgaagccg
      301 aaggaagatc agcagggacc tggtatcgga ccgcaggaat ttgattttct agaagtcttc
      361 gaagatatgg gctcaggcat tatcttcaga cgaggatgac acgggcaggt tcaaaaccgt
      421 taaagttggg aacggcgctc gctgaggaat aagcgccgat atttttgaca taaaccacat
      481 cgcccacaaa gagttcggga acttcttcat tggaggaaag aatatcgagt gagtcacacg
      541 tggggcccgc gaatacactc atgtattttt gtccgcgccg caaagtttta aactcatatt
      601 tgcagtgatc aaacatcatc cctgaaaaat cctggtaaac cccgtcattt aaataataga
      661 aatttttatg atccctgaaa gccttgccga caacctgggt caccagcacc cctgccggcc
      721 ccacaaaaaa acgccccggc tccgccataa attgagtcga ttttggaaaa agctccctca
      781 tctgcctgcg aatttgaccc gccatgcgct caaagttgat gccaatctcg ttatcaaaat
      841 gctgaatggg aaatccccca ccgatatcca caatcgaaag cggtatgccg ctttccttcg
      901 attctttaaa aattcccgcc gtaagttcga gcgcttgcag ataattttca acattggtgg
      961 actgcgaccc tacgtggaaa ctcacccctt taggaacgag gccaagcccc ttcgcttttc
     1021 tcaacagata aaagccctgg tcggggtcag ctccaaactt gagcgatagt tggaccacgc
     1081 taccccggtt cgccacccgg atacgaagga tcacatgcgc tccgggataa ttttccgcta
     1141 ttttataaag ctcggcctca ttatcaaagg tcatcagccg gacacgacgt ttccgcgccg
     1201 tgacaagatc ttctttggat ttgatcgtgt tcgcgaaaat aatatgtctc ggctttgcgc
     1261 cctgttttaa aaccagtttc atttccgcgg cgctcgccac gtcaaaacaa gtcccgagct
     1321 tcgcgaaaaa tttgacaatc ctcgggtgcg gattcgcctt aatggcgtaa tacggagtca
     1381 cttggggaaa ggctttctgg aaacgagaca attgcttttc aagaacactt ttccggataa
     1441 tcatgaacgg agtcttgtgt ttcttgagca tttgccgaat caaattctcc actccggaca
     1501 actcggcggg tttgaccggc ttgtttaaaa ggggcgcatg ttcgttcaat ggaatcggtt
     1561 gcttcattct catctaaact ctttcgtcca caagaaaaga attaaactgc caaagatcgg
     1621 actccctggg ggctttggct ttaaacttga gatatttgtg gttaaggttc ttgagcggcg
     1681 tccagtccga agggatcgac acacaaggcg cgacgtaagg cgtcgcgaac ttcaggatgg
     1741 cttcatggtc aatgtcatcc ggaagcaaaa acccttcccg tggattttgg atcatccaga
     1801 gcgcggccgc cacaactgat gccgcgacct gcaaggtcgt cgcctgctgg tggggaacca
     1861 atttccgggc cgtgtggata tcaaggagcg aaccgcgcac caggatttga agtcatgccc
     1921 cattaaaaga actcccagct cgtcccgccc gtcaataata tcatcggaca ggatccgttg
     1981 tttttcctgc atcttgtact gccgcatcct gagttcatga agcgatagaa gggcggaatc
     2041 cgacgggcaa taagcgtagt gaactgtcgg gcgataaaca gctttcccgt ttttccagac
     2101 cgtcaggcga tccgaaatac tgaacgcctc gccatgacga ataaccatcc cgttgatctc
     2161 cccgcaaggc acccaggaac ggacaaacgt gttcatcccg agcgtcccca gacaaatctg
     2221 gtttttggga cccgtcttgt gcgcatagcc gtctttggga agtttcctct cgtgagttcc
     2281 ccaacccatc tctgccggag caactccttc ttcgtaaaat ccttcgatcg accaggtgtt
     2341 cacaaactcg ttcacccgtt tcgggacatc ggtcacctgc gtatcacgct cgctgatatg
     2401 gatgaccttg acccctgtta tctgcgcgag tttcgcaaag tttttttccg cgataaaccg
     2461 ctcaagattt ttcactcgag aatctcgcgg cttctcccga ataatttttc tcgcgatatc
     2521 aatcaacgcc tttttcgtga aatgcgacac gagcccaggg tttgccccgt ggtccacaat
     2581 cgccgtggag cctttttcac tgccccattt tctcgcgatc tgtttccgga gaatcatttg
     2641 acgagcatac agggtatact tcgtcgggtc ggtccgtttg tcatcacggt agggatccca
     2701 ctcttctacc gaggtgttca cgtaaagcac ctgattcgtc cgacaccaat ccaggatctc
     2761 ccagcatccg atattccagc caagatcgat gatcatgtcc cccgaaccca catatcgcgc
     2821 gaggagcgtt ttgtaattcg cttcagtcac tctgtcaaaa acgtatcgca cacccttttt
     2881 aagggcatct ttcacccgcg tgcggttgtc gacaaaatcc atgatcgtga ttttttttgc
     2941 cggcatgtca aagtgacgaa ggatcagcgg aacggcgcac tgagacacgc ctccgcaccc
     3001 aatgatgaga actttcccgc caaatggcac acgagtttta gggatgcgcg ccgaagcttt
     3061 cggataacga aacaatgttt ccattttttc cctctgattt tcgcggttga gcaaaaagga
     3121 cagtccctta tatgtgaata aagactgtcc tcagtttgtg attcaatgac ccttcggatt
     3181 tttttctaaa ggcgtccttc agggtgagcg gaccgggggt ttcccgacct ctcaatttat
     3241 aaatgagtgg gaatgctaca acaaataaac gttagcttca accctttaaa cagaaacctt
     3301 caaatattcg tccgcgttga gccatcagat cttttccccc cggacaagat cctggtaggt
     3361 ttcccgttga cggaccaccc ggaaagacgc tcccttcaca agcacctcgc acgcacgcct
     3421 ccgggaatta taattcgagc tcatcgtaaa cccgtaagcg cctgaggtca tcaccgccag
     3481 aaaatctcca gacctcattt cggggagctc acgatccttc gcgaaaaaat ctccgctctc
     3541 gcagatcggg ccgacaacgt cataaaccca ccgagcgaca tgctcctgtt tgcggatcgg
     3601 ccaaatcccg tggtaggcat tataaaaggc cgggcgaatg agatcattca tacccgcgtc
     3661 cgtaatcaga aattttttca cggccgtctc cttgacgtaa agaactttcg cggccagcac
     3721 gccggcattc gccacaatga atctgccagg ctcaaaaata atctgatatt ttcgtttctt
     3781 gaagagaggc aggatttttt tggcaaactc gtccgccgtt tgagggtttt catcactata
     3841 gatcacacca agcccgccac ccaagttcag aaatttaatc ttgatccccg ccaactccag
     3901 atcatcgaca aattccagaa ttttacggaa cgcttcaaca aaaggctccc ccgtttcgat
     3961 ttgggaaccg atatggacat gcaaaccaca cagtgtcgcg tgggtcagct ttttgctttc
     4021 aagataaatg gaacgcgccc ggtcaaaatc aattccgaac ttagtttcag ctcttcctgt
     4081 cgtgatatag gcatgcgtgt gcgggtccac attgggattg accctgagag agacattcac
     4141 ttgttttcgc atcgctttcg cgatctgatg gatcctctca agctctggga gcgactcgac
     4201 attgaaaaga aggatccgcg cgcggatcgc ttctcgaatt tcctcatccg tttttccgac
     4261 gcccgcgtaa acgatcttcc ttcccgggca ctggaccttc ctcgcccgaa aaagttcgcc
     4321 tcccgacaca atatcaagcc cggctccgtt tttgaccaga gttttgagaa cagcaagatt
     4381 ggaattcgct ttcaccgagt agcaaatgag gggccgaacg gatcgaaatg ccttctgaat
     4441 cttcgcgaga tgttctgaaa tcgtcctgga gctgtagata taggccgggg taccggtttt
     4501 tttgaggatc ttcttgaggg gtactttctc gcaacaaagg tcgcccttcc tatcataata
     4561 aaaatcgtgc atgcgagttt ctccctgaaa ttcacctcgt agcatgactt ttcacctcat
     4621 cttcctgatg atccggcaaa tcaaagacag cgttcccacc gtttcagctg tttttcaacc
     4681 tgatccggat gcgtactccc gattgttttt ttacctttga cggacgccgc cgcgtcaaat
     4741 aggtcaaaaa cctcggcccc gatccgcgct gaaaattttt tccagtcatt taaagagaat
     4801 tcccgcaact tcttccccga gtccgccgca taacgaacgg ccttccccac gatagcgtgt
     4861 gattcgctga acggaacctt cttccgcacc aaatactcaa ggatgtccgt cgcgaaaagg
     4921 gtgtcatctt ccagcgaatc gagaatgttc ttcttgttcc atttcaggga atcaatcgtc
     4981 aggctcaaaa cctcaagcgc gattgtcgtt ttgtggatcg cgtcaaaaac tcccggcttg
     5041 tcttcctgga ggtctcggtt gtaggaaagc gccaaccctt tttgaatggt taacatcgct
     5101 tggagatgtc caaagacaac accactcctc cctctcacaa gttcaaagac atccggattt
     5161 tttttctgcg gcataaggct tgatccggtc gcaaacgaat cgtccagctc cacgtagccg
     5221 aacgcttctg aattccagag aataaaatcc tccgcgagac gcgaaagatg catccagaga
     5281 atcgagagat ccgccaaaaa ctccgccaaa aatcctcgat cactcaccgc ggccatactg
     5341 ttggccgcga tcttttcaaa tccaagctct ttcgccaaaa acttttgatc gatcggaagc
     5401 gccgacccgg caagagccgc ggcccctatc ggcatgacgt tcacgcgctt catcaaatca
     5461 caaagccgca acctgtcttc ttcaagcatt tcgacataag caaggaggtg atgggcgagc
     5521 agaactggct gggccttcct caaatgcgtc atccccggaa caatcagatc gtgagcccgc
     5581 cgggccgccg tcatcaggga tttctgaagt cgctggatct catattgaat gacgagcacc
     5641 ttccccctga ggtagatcct tgtagaggtc acgacaagat cgttccgact ccggccagtg
     5701 tgcagtttct tggcgaccgc gcccaccttc ttctcaagct ccgactgcat gagggtatgg
     5761 atatcttcat agcgatccgc gtacaaagac aaatcttgtc cctccagcat cttggcaact
     5821 gcggcgaggc ctttttggat cgcacgggat tctttttccg tgatgattcc ggtcctggcg
     5881 agcatttttg cccacgcctc attaacgaga acctccgatt caaaaagctc cttatccgtc
     5941 ttcaaagaat agctaaaaca ctggagcgcc ggatgcattc cttttttgaa ccgccctccc
     6001 caaagtttct ttgtcacatc aatc
//
LOCUS       DAOSIO010000100         5968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_11995_length_5968_cov_5.857095, whole genome shotgun sequence.
ACCESSION   DAOSIO010000100 DAOSIO010000000
VERSION     DAOSIO010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11995_length_5968_cov_5.857095"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PK590_04715"
     CDS             complement(<1..462)
                     /locus_tag="PK590_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOG23932.1"
                     /translation="MPDFSQHHVVFQSLLAGLFTWGMTGLGAATIFAFRKINQKILDV
                     MLGFAAGVMVAASFWSLLAPSIEMAADQGMIPWFPALTGFLLGAVFFRGIDKILPHLH
                     PFMPTETAEGIKTTWPKSTLLVLALTIHNIPEGLAVGVAFGSTGAGAHSLPL"
     gene            complement(551..1042)
                     /locus_tag="PK590_04720"
     CDS             complement(551..1042)
                     /locus_tag="PK590_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOG23933.1"
                     /translation="MGTKGKEIVGVNVDELLKLLNKAYADEWLAYYQYWVGAKVAAGR
                     MRGIIAGELKEHAEEELRHADMLVERMLQLGGKPLLTPEAILKETNCGYDAPEDPCTK
                     KLLAQNIKGEQCAIATYKKLYDFVKGKDPITEKIILEILEDEVEHEEDLEAILDDMKT
                     PVA"
     gene            complement(1185..1892)
                     /locus_tag="PK590_04725"
     CDS             complement(1185..1892)
                     /locus_tag="PK590_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG23934.1"
                     /translation="MKITLIAEGSTKKDREALRWGISFLVGNILFDTFGRADIFEENV
                     CRYKIDLSRVRHVVISHEDWDHIAGLENLLRERPKMKVFLCKKTGPVLKKIVRDSGGS
                     LTLVDSPRRIAPGVFSLGQMRANTGRGILYEQAVVAKSREGFSVITGCAHPGIVRIIK
                     RAIRCFGKNIYAVCGGFHMKDNSREENLGIIGELQRLGVKKVIPLHCTGAPAFRLFKR
                     VFKNNCLRLKRGGTLKL"
     gene            complement(1920..2279)
                     /locus_tag="PK590_04730"
     CDS             complement(1920..2279)
                     /locus_tag="PK590_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOG23935.1"
                     /translation="MNVAVVGASDKPKRYAYMAVRLLLEKGHTVFPVHQRVREIDGLR
                     VYPSLGNITAPIDTITLYVGKSTSAAMAADILACRPRRIIFNPGAENEALERQAKKAG
                     IQTVRGCTLVMLRTGQF"
     assembly_gap    2489..2588
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2589..3387)
                     /locus_tag="PK590_04735"
     CDS             complement(<2589..3387)
                     /locus_tag="PK590_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG23936.1"
                     /translation="MKQILVISGKGGTGKTVLTGALAALAQNKVMADCDVDAANLYLL
                     LNPSIREKHPFQSGASAVIDPALCARCGQCSAACRFDAISRDFVVNPIACEGCGLCAR
                     VCPAGAVIMKENTAGDWFISETRYGPFVHAKLGLAEENSGKLVAKVRETAREIAESKA
                     ADYVIIDGPPGIGCPVVASLSGVDLAVIVTEPSLSAIHDAERVMDLAKHFEVPVKLVV
                     NKWDINPAATEDIQRLCDSRHVPLIGLIPFDRVVVESVLAGEPVTERA"
     gene            complement(3384..4226)
                     /locus_tag="PK590_04740"
     CDS             complement(3384..4226)
                     /locus_tag="PK590_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013036750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG23937.1"
                     /translation="MIISVASGKGGTGKTTVAVNLALALGDCQLLDCDVEEPNAQVFL
                     KADIRAREPATVRVPEIDYQKCDFCGKCGDVCAYHALAVLPRKVLFFPQMCHSCGACE
                     MLCPRKAIRETDRTIGEIETGDREGIRFVQGKLNVGETMAPPLIRQVKSRIEPNSVAI
                     LDAPPGTSCPMVTAVRGSDFCILVTEPTPFGMNDLHLAIETVKKLGIPYGVVVNRAEA
                     DDLKTRDYFKSNQIPLLLKIPLDRKIAELYSIGTPFIGPMPEYREKFRALFKTISETV
                     RVSS"
     gene            complement(4356..4721)
                     /locus_tag="PK590_04745"
     CDS             complement(4356..4721)
                     /locus_tag="PK590_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOG23938.1"
                     /translation="MKIAITSEGQTLDAAVDPRFGRCGYFLIIDTDTDKVEALQNPGA
                     QQTGGAGIQAGQLMASRGVEVVLTGNMGPNAFQVIQSAGIKVVTGVSGKISEALQAYK
                     DGKLLPAENPSVGSKFGVS"
     gene            complement(4823..5188)
                     /locus_tag="PK590_04750"
     CDS             complement(4823..5188)
                     /locus_tag="PK590_04750"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOG23939.1"
                     /translation="MRIGITLNDDRGLESDVCAHFGQCRYFGLVDLEGQNIKNVTVVP
                     NTAQHGGGGCQAVDVILSHKITHVIAGGMGGGAQMKFSNAGIPVFGFTGKAGDAVREF
                     LKQNLGGLNACAEHDGGCH"
     gene            complement(5258..5551)
                     /locus_tag="PK590_04755"
     CDS             complement(5258..5551)
                     /locus_tag="PK590_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOG23940.1"
                     /translation="MPGGDRTGPAGTGPMTGWGRGSCAGGRGLGRAGGWGGRGWRHRF
                     YATGLPGWQRNSPFQPVVERRDESASLREQADYLSAELEAVRARLSELEQNKE"
     gene            complement(5581..>5968)
                     /locus_tag="PK590_04760"
     CDS             complement(5581..>5968)
                     /locus_tag="PK590_04760"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOG23941.1"
                     /translation="SGEKIMPRPFKCRRVLGMPKSDYLKPRGIPLSDLREIDLTIDEF
                     EAIRLADLEGLYQEDAAKKMNISRQTFGNIIAEARKKVADAIVNGKALKIRGGAYVRS
                     LPPVPPNFRGGFGGGRGRGRCRRGNF"
BASE COUNT         1254 a         1669 c         1561 g         1384 t
ORIGIN      
        1 gagcggcagg gaatgagcgc ccgcgccggt cgatccgaac gccactccga cggccaatcc
       61 ttccggaata ttgtggatcg tgagcgccag gacgagaagg gtgcttttgg gccaggtggt
      121 tttgatacct tccgccgttt ccgtgggcat gaagggatgc agatgaggaa ggatcttgtc
      181 aatcccccga aaaaaaacag cgccgagaag aaaaccggtc agggccggaa accagggaat
      241 catcccctga tcggccgcca tttcaatcga gggagcgagg agcgaccaga aactcgccgc
      301 gaccatcacg cccgccgcga aaccgagcat gacgtccagg atcttctgat tgatcttccg
      361 gaacgcgaag atcgttgccg cgccgaggcc ggtcatgccc caggtaaaaa ggccggcaag
      421 caatgattga aaaacaacgt ggtgttgact gaagtcaggc ataagggatt tttaggaggg
      481 accaacgcgg aatcaggcgg ggacggttct ctgaaatgaa agccgtcccc gcgagaatcc
      541 gtctccgtaa tcaggcgacc ggagttttca tgtcatcgag gatcgcttca agatcttctt
      601 cgtgctcgac ctcatcctcg agaatttcaa ggatgatctt ttccgtgatc gggtctttgc
      661 ccttcacgaa atcgtaaagc tttttgtacg tcgcgatcgc gcactgctcg cccttgatgt
      721 tttgcgcgag caattttttc gtgcagggat cttccggggc gtcgtaaccg cagttggttt
      781 ccttcaaaat cgcttccgga gtgagaagtg gtttgccacc aagctgaagc atgcgttcga
      841 ccagcatgtc cgcgtggcga agctcttctt ccgcgtgttc cttaagttcc ccggcgataa
      901 tgccgcgcat ccgtccggcg gccactttcg cgccgaccca atactgataa taagccagcc
      961 attcgtccgc gtaggctttg tttaacagtt ttaacagttc gtcgacatta acccccacga
     1021 tttcttttcc tttggtaccc atgatctgat tctccttttt tctgtaattt cggcgaaggg
     1081 tgttcaggca agcccccgct gattgggcca ttttactgta gaaacgctaa ttcaaaaatt
     1141 aaaattctcc ctcctttgct ttatttggat ttttgaaatt ttatttaaag tttcagggtg
     1201 ccgccccgct tcagccgcag gcagttgttt ttaaaaactc gcttgaataa acggaaagcc
     1261 ggcgccccgg tgcagtgaag agggatgacc ttcttgacgc ccaggcgctg gagctcgccg
     1321 ataattccca ggttttcttc acgggagttg tctttcatat ggaaacctcc gcaaacggcg
     1381 taaatgtttt tcccgaaaca ccgaatagcc cgtttgatga tccgtacgat tccggggtgc
     1441 gcgcaacccg tgattacgga aaatccttcg cgggatttcg cgactacggc ctgttcataa
     1501 agaatgccgc ggcccgtgtt cgcgcgcatc tgcccgagag aaaagacgcc gggcgcgatc
     1561 cggcgcgggg aatcgacaag cgtgagcgat ccgccgctat cgcggacaat ttttttcaaa
     1621 acgggtccgg tttttttaca aagaaaaacc ttcattttcg gccgctcccg gaggaggttt
     1681 tccagtcccg cgatatgatc ccagtcttcg tgagagatca caacatgcct gacccgggag
     1741 aggtcgatct tgtagcgaca aacattttcc tcaaaaatgt cggcccggcc aaaagtatca
     1801 aaaagaatgt ttccgacaag aaacgagatt ccccagcgaa gcgcctcgcg atcctttttt
     1861 gtggagcctt ccgcgatcag agtgattttc ataaatgtcc caatattttg aaaaaaattt
     1921 taaaattgcc cggttctaag catgaccagc gtgcagcccc gaaccgtttg aatgccggcc
     1981 tttttggcct gtcgctcgag ggcctcgttt tccgcgcccg gattaaaaat aatacggcgc
     2041 gggcggcagg ccagaatatc cgcggccatt gccgccgatg tcgatttacc cacgtacagg
     2101 gtaatggtgt cgatgggggc tgtaatattc ccgagggacg gatacaccct gagcccgtca
     2161 atctcgcgca cgcgctgatg gacggggaac acggtatgac ctttttcaag cagtaacctg
     2221 acggccatgt aggcatatcg tttcggttta tcagaagctc cgacgacggc aacattcatg
     2281 attttcccaa agggttatgc gccagatgtc tttcagacac cgcgatgacg gaagaaaaat
     2341 atttcagcgc gtaaagatta cgttcaaaac gagacatcgt tcaatcccga atggcctccc
     2401 agattttgat gaattcttct tttaaggggc cgccggcgcg ttcggtgacg ggttctccgg
     2461 caaggacaga ttccacgacg acacgatcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnncg gcgcgttcgg tgacgggttc tccggcaagg acagattcca cgacgacacg
     2641 atcgaaaggg atcagcccga taagcggaac gtgccgcgag tcgcagagcc tttgaatatc
     2701 ttccgtggcg gcgggattga tgtcccattt attgacgacc agtttgaccg gcacttcaaa
     2761 gtgtttcgcg agatccatga cccgctccgc gtcgtggatt gccgaaagcg acggttcggt
     2821 gacgatgacc gcgagatcca cgcctgaaag cgacgcgacc acggggcacc cgatccccgg
     2881 gggaccgtcg atgatcacat aatcggcggc ttttgattcg gcgatttccc tggcggtttc
     2941 gcggactttt gccacaagct tccccgagtt ttcttccgcg agcccgagtt tggcgtggac
     3001 gaaagggccg taacgcgttt cagaaataaa ccaatcgccg gccgtgtttt ctttcatgat
     3061 gaccgcgccg gcggggcaca cgcgggcgca aagtccgcat ccttcgcagg cgatcgggtt
     3121 gacgacaaaa tcccgggaaa tggcatcgaa gcggcaggcg gccgagcatt gcccgcaacg
     3181 ggcgcaaagc gccgggtcaa taacggcgga agcgccgctt tgaaacgggt gtttttcccg
     3241 gattgaaggg tttagcagta aataaaggtt cgcggcgtca acatcgcagt ccgccattac
     3301 tttattttgg gccagcgcgg ccagggctcc ggtcaatacg gtttttccgg taccgccttt
     3361 tccgctgatc acgagaattt gtttcatgac gacaccctga cggtttcgga gattgtttta
     3421 aaaagggccc tgaatttttc gcgatattcc ggcatcggcc cgatgaacgg ggttccgatg
     3481 gaataaagtt ccgcgatttt tcgatccagg gggattttga gaaggagcgg gatctggttt
     3541 gacttaaaat aatcccgggt tttcagatcg tccgcttcag cgcggttcac cacaacgccg
     3601 taggggatgc caagtttttt gacggtttcg atcgccagat gaagatcatt catcccaaac
     3661 ggggtcggtt ccgtgaccag aatacaaaaa tcacttcccc ggaccgccgt gaccatgggg
     3721 caggaggtcc cgggcggcgc gtcgagaatg gcgaccgaat ttggctcaat ccgggattta
     3781 acctgccgga tgagcggagg cgccatcgtt tcacccacgt tcagcttccc ttggacaaaa
     3841 cgaatgccct cccggtcccc ggtctcgatc tcgccaatcg tccggtcggt ttcccggatg
     3901 gcctttcgcg gacaaagcat ttcgcaggcc ccgcaactgt gacacatttg gggaaaaaac
     3961 aggaccttcc tggggaggac cgcgagcgcg tgataagcgc agacgtcccc gcattttccg
     4021 cagaagtcac atttttgata atcgatttcc ggaacgcgga cggtcgccgg ttcccgcgcg
     4081 cggatatcgg ccttcaaaaa aacctgcgcg ttcggttctt caacgtcgca gtccagcagt
     4141 tgacaatctc cgagcgcgag cgcgagattg accgcgacgg tagttttacc ggtcccgccc
     4201 ttgccgctcg ccactgaaat aatcatcggg gtttctccac ggtcagaatt tgctgaaaac
     4261 ggtaacgcgt tttttcaaga taaaatcttt tttcaggaca gacttcgatc cccgccaggg
     4321 gcacggcgta aaccggcgct atccagctcg accaatcagg acacgccgaa ttttgagccg
     4381 acgctgggat tttctgccgg caaaagcttg ccgtctttgt aggcctgaag ggcttccgat
     4441 atttttcccg agaccccggt cacgaccttg atccctgccg actgaataac ctgaaaagcg
     4501 ttcgggccca tatttcccgt cagtaccact tcaactcctc ttgaggccat aagctggcca
     4561 gcctgaatgc ccgcgcctcc ggtttgctgc gcgcccggat tttgaagtgc ctcgaccttg
     4621 tccgtatcgg tatcgataat cagaaaataa ccgcatcgcc cgaaccgcgg gtcaaccgcc
     4681 gcgtcgagag tttgtccttc cgaagtaata gcgattttca taaagtgtcc ccgaatagtt
     4741 tgaatttact ttttgttttt atcaaaaggc gaacttgttg tttcgccggt ttgtgacctg
     4801 aggcccacgc ggctccgtgt agtcagtggc atcctccgtc gtgttccgcg caggcattga
     4861 gcccgccgag gttctgcttc agaaattccc tgaccgcgtc gccggccttg ccggtgaagc
     4921 cgaaaacggg gattcccgcg ttcgaaaatt tcatctgggc tcctccgccc atgcctcccg
     4981 cgatgacgtg agtgatcttg tgacttaaaa tcacgtccac ggcctgacag ccgcctccgc
     5041 cgtgctgggc ggtattggga acgaccgtga cgtttttaat attttgaccc tcaaggtcaa
     5101 ccagcccgaa atagcggcat tggccgaagt gggcgcacac gtcgctctca agtccccggt
     5161 catcgtttaa agtaattccg attctcattc ccgttcctcc ccgtttcctt tgaaaagggg
     5221 acaggcccgt gttgaaagcg tctgtccccg tgaatgttta ttctttgttt tgttccagtt
     5281 cgcttaggcg ggcgcggacc gcttcaagct cggcggaaag atagtccgcc tgctccctga
     5341 ggctcgcgct ttcatctctc cgttccacta ccggctgaaa gggagaattt ctttgccaac
     5401 ccggtaatcc ggtcgcgtaa aaccgatgtc gccagccccg gccgccccaa ccgcctgccc
     5461 ggcccaaacc gcgaccgccg gcacaagaac ctcgtcccca acctgtcatc gggcccgttc
     5521 ccgcgggtcc cgttctgtct cctcctggca ttttagtcac ctccttgtgg ttaccgcttt
     5581 tcaaaaattg ccacgccggc atcttccccg gccccggccg ccgccgaacc ctccccggaa
     5641 atttggggga acaggcggaa ggctccggac ataagcgcct cctctgatct tgagcgcctt
     5701 gccgttgacg atggcgtccg ccaccttttt tctcgcttcg gcgatgatgt tcccgaacgt
     5761 ttgtcttgaa atgttcattt ttttcgcggc gtcttcctgg taaagcccct cgaggtcggc
     5821 aagccggatc gcctcaaatt catcaatggt gagatcaatc tcccgcaggt ccgaaagggg
     5881 aatcccgcgc ggcttgaggt aatccgattt cggcattccc aaaacccgtc ggcacttaaa
     5941 aggacgcggc ataattttct ctcccgat
//
LOCUS       DAOSIO010000101         5913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_12163_length_5913_cov_4.269546, whole genome shotgun sequence.
ACCESSION   DAOSIO010000101 DAOSIO010000000
VERSION     DAOSIO010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12163_length_5913_cov_4.269546"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PK590_04765"
     CDS             <1..585
                     /locus_tag="PK590_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HOG23942.1"
                     /translation="SSVTRAVREVPVTVKMRTGFTDPSGKEALHLAQLAEACGLSAIT
                     VHGRTRAQGYSGKADWNVIKMVKRAVTIPVFGNGDIFTARDARRIKKDTGCDGIAIGR
                     GALGNPWIYENIRSAFGHKKIHRPSLVEIRRTVREHLRLNARFDGEKVAHLKARHIAS
                     WYIKGLPGASRLREKINRSRSLDEIIALVESHDS"
     gene            complement(625..1515)
                     /locus_tag="PK590_04770"
     CDS             complement(625..1515)
                     /locus_tag="PK590_04770"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HOG23943.1"
                     /translation="MKKKSAKDLQNIIAIVYDFDGTLAPGNMQEETIFREYGIDKRKF
                     WAKSRALVRQKGYESTLAYLRLLISDPVFKERPLDRKTLKRLARQLPYYPGVAQGYFR
                     QLTSFMNKIPEVREWGITVEHYIISSGLSELLDGASIRKHFKKIYACEYDYENGRPVF
                     PKLVINDTNKTQFLFRINKGKLELSESINQHMPAGKRRIPFANMIYIGDGVTDVPSMT
                     VMNKSGGHAIAVYDPNEKVPREVKKMIAEKRAEHFAPADFRRGSLLIKILHRTLKQII
                     HSISFRLSSRRSSDWVRRHW"
     gene            complement(1542..1919)
                     /locus_tag="PK590_04775"
     CDS             complement(1542..1919)
                     /locus_tag="PK590_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23944.1"
                     /translation="MEKYKNIIIAGIVAVALIVSALMIQSGMVRAAKVNPAEAYTLHG
                     YEGVLYRLNTITGRLDVLVPSNEAALLFPIGQFQLPRADATLTDQEKTSLSSNIRTIA
                     QYIQGERARSLGISQDEAKSVKS"
     gene            complement(1992..2537)
                     /gene="orn"
                     /locus_tag="PK590_04780"
     CDS             complement(1992..2537)
                     /gene="orn"
                     /locus_tag="PK590_04780"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HOG23945.1"
                     /translation="MSAKTHRFNMVWIDMEMTGLDPVKDRIIEIASIVTDGQLNILAE
                     GPNLVIHQPERLIRGMDQWNQEHHSKSGLIADVRNSTVTVKAAEKMTLEFLKEYCKPK
                     KAPLCGNSVHHDRRFLARDMPAIDDYLHYRHVDVSTIKALVARWYPKDKEPPKGKSHR
                     ALSDIRESIEELRFYRERYFK"
     gene            complement(2534..3091)
                     /locus_tag="PK590_04785"
     CDS             complement(2534..3091)
                     /locus_tag="PK590_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23946.1"
                     /translation="MKKNKETLGKNARLLIDFVLDSSAHELVYNGIFRKNKGAVKSDM
                     TKFLQDFVPAKLALGCMFWNQCCEAHGLEAKEIRNLYFLEVMKRFETPQSVDVATRFS
                     ECLYAVNARPEESPVLSVTSHLFGKLGLKCAEGEETDAVISEAFLFAMEVNEALKNAF
                     ENEFDELFYANENFHVPETEQKGSL"
     gene            3228..4226
                     /locus_tag="PK590_04790"
     CDS             3228..4226
                     /locus_tag="PK590_04790"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOG23947.1"
                     /translation="MARRRRKKIKNPFKIKIKVNKKRLKKALWVLSIVMAGYLLFLGT
                     QYLVRSVRKPQPASPVITRPTTTTRSPKVTQVTQATQVAQPATLSRKKPVSAVTAQVP
                     PPAPIKLTRPSIVIVIDDIGAHNKLRGEMERLGNKVTYAILPLLPYSRFFSELATRKN
                     AEAILHLPLDTTQDMIPGRGLIVSTMPREQALDMLSRDIASVPGIVGVNNHMGSRGTA
                     DREMMTLILEELHRRGLFFLDSYTTKDSVVPSVARQIGIPFLQRGVFLDNVDSPEAIR
                     GQIRETEAVAKKQGYAIAIGHYRKNTLDVLFREIPRLEKKGFAIISLSRLLQSRRR"
     gene            complement(4242..5693)
                     /locus_tag="PK590_04795"
     CDS             complement(4242..5693)
                     /locus_tag="PK590_04795"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOG23948.1"
                     /translation="MKYQEVVAKKVLIRTFGCQMNVYDSQVAAGLLEREGFEVLQDSA
                     EVEPDPAGCLSGGADIVIMNTCSVRDHAEQRVWGRLGRLGKEKKERPELIIALMGCMV
                     EEHREKLFKRFPYLDLILGTRNISELPALVRRIQATRECVSRISRDGISIEYSEFTKR
                     DSPYHAWLPIMTGCDKECTFCIVPKTRGREVSMPAEDVIREAKRLVADGVKWITLLGQ
                     NVNSYGAKERGVAGTDPGDVSLRTPDAVPLAGAQNVSFPQLLELLCAIDGLERLSFTT
                     SHPGDATEELFQVIAKNPKISRRFHLPLQSGSDKILRRMKRLHTFADYKARVDRLREL
                     VPGISVTTDIIVGFPGETEEDYLATKRAIEEIRFDSAFIYKYSVRPGTPAERLTDDVP
                     FETKSRRNNELLAIQRSVTKAKNLERVGREVSGFVEARAPKGEQSFVARTDFDGRVIF
                     ACEKDLIGEFVRLTLDSVSHETFHGTLVPRGDA"
BASE COUNT         1362 a         1764 c         1427 g         1360 t
ORIGIN      
        1 tcatccgtca cgcgcgccgt acgcgaagtg cccgtcacgg tcaaaatgcg aactggtttt
       61 accgatccca gcgggaagga ggcccttcac ctcgcccaac tcgccgaggc ctgcgggctt
      121 tccgccatca ccgttcacgg acggacccgg gcccagggat attccggaaa agccgattgg
      181 aacgtgatca aaatggtcaa gagagcagtc acaatcccgg ttttcggcaa cggcgatatt
      241 tttacggccc gcgacgcgcg ccggatcaaa aaagacaccg gttgtgacgg gatcgcgatc
      301 ggccgcggcg cgctcggaaa tccgtggatt tacgaaaata tcaggtcggc attcgggcac
      361 aaaaaaattc accgtccgtc cctggtcgaa atcaggcgca cagtccggga gcacctgcgc
      421 ctcaacgcgc gatttgacgg ggaaaaagtc gcgcatctca aggcgcgcca catcgccagc
      481 tggtatatca aaggacttcc cggcgcttct cgcctccggg aaaaaatcaa ccgtagccgc
      541 tccctggacg aaattatcgc cctcgtcgag tcccacgatt cctgacactg aacggagccg
      601 accggaacga ttacctgcgg ggttttacca gtggcgcctt acccagtcgg acgagcgccg
      661 ggaggaaagg cgaaacgaga tggagtgaat gatctgttta agggtgcggt gaaggatttt
      721 gatcaggagg gacccccggc gaaaatcagc cggggcaaaa tgctccgccc ttttttcagc
      781 gatcatcttc ttcacttcgc gcgggacttt ttcattcggg tcgtagaccg cgatcgcgtg
      841 accgccgctt ttattcatga ccgtcatgga cggcacgtcc gtgaccccgt caccgatata
      901 aatcatgttg gcgaacggga tccggcgttt cccggccggc atgtgctgat tgatgctctc
      961 gcttagctca agctttccct tgttgatcct gaaaagaaac tgcgtcttgt tggtatcgtt
     1021 aataacgagc ttcggaaaaa ccggccggcc gttttcataa tcatattcgc aggcgtagat
     1081 ttttttaaag tgcttgcgga tactggcgcc gtcaagcagt tcgctcaggc cgcttgaaat
     1141 gatgtaatgc tcaaccgtga tcccccactc ccgaacctcc gggatcttgt tcatgaagga
     1201 agtcagctgt ctgaaataac cctgggcgac tcccgggtaa tacgggagtt gccttgccag
     1261 cctttttaga gttttccggt caagcggccg ctccttaaat accggatctg aaatcagaag
     1321 gcggagatag gcgagagtcg attcatatcc tttttgacgg accagcgccc tggatttcgc
     1381 ccagaacttg cgtttgtcga taccgtattc ccggaagata gtctcttcct gcatattgcc
     1441 gggcgcgagg gtcccgtcaa aatcatagac aatcgcgata atattctgaa gatccttcgc
     1501 agactttttc ttcataaagt cccgttgcgc tcttcctgcg tttatgactt gaccgacttg
     1561 gcctcgtcct gcgaaatccc taaactccgg gcgcgctctc cttgaatgta ctgggcgatc
     1621 gtgcgaatat tcgaagataa acttgtcttt tcctgatccg tcagcgtcgc gtccgcgcgc
     1681 ggcaattgaa actgccctat cgggaacagg agcgcggctt cattgctcgg gaccaagacg
     1741 tccagccgtc cggtgatcgt gttgagccgg taaagaacac cttcgtatcc gtgcagcgta
     1801 taggcttccg ccgggttcac cttggcggct ctcaccattc cggattggat catcagcgcg
     1861 ctgacaatca aggccaccgc gacaattccc gcgatgataa tatttttata cttttccata
     1921 ctccctcctc ttcaaagggt taaaaaaggt ttaaacgggg ctggttgaaa tgtgataaag
     1981 gttgtcccgg cttatttaaa ataacgctcg cgataaaagc gaagctcctc gatcgactcg
     2041 cgaatgtccg aaagcgcgcg gtgtgatttc cctttcggcg gttccttgtc tttcggatac
     2101 catcgggcaa ccagcgcctt gatcgtgctg acgtccacgt gacgataatg cagataatca
     2161 tcaatcgccg gcatgtctcg ggcgaggaac ctccggtcat gatgaacgga gtttccgcaa
     2221 agaggcgcct ttttgggctt gcagtattcc ttcaaaaact caagcgtcat cttctcggcc
     2281 gccttgacgg tcacggtcga atttctcacg tcagcgatca gacccgactt cgagtgatgt
     2341 tcctgattcc actggtccat cccccgaatc aggcgctccg gctggtgaat gaccaggttc
     2401 ggtccctcag ccaggatatt cagctgaccg tccgtgacga tcgaggcaat ctcgataatc
     2461 cggtctttca cggggtccag ccccgtcatc tccatatcga tccagaccat gttaaagcgg
     2521 tgtgtcttcg cgctcataaa ctgcctttct gttcggtctc gggaacgtga aagttttcgt
     2581 tagcgtaaaa aagctcgtca aactcatttt caaaagcgtt cttgagagct tcgttcactt
     2641 ccatcgcgaa aagaaacgcc tccgagatta ccgcgtcggt ctcctcccct tcggcgcact
     2701 tcaggccgag cttcccaaac aggtgcgacg tgacggaaag cacgggcgat tcctcgggac
     2761 gggcattcac cgcgtaaaga cattcgctga accgcgtggc cacgtccacc gactggggcg
     2821 tctcaaagcg tttcataacc tcaagaaaat acaggttcct gatctccttc gcctcaagcc
     2881 cgtgcgcttc acaacactgg ttccagaaca tacaaccgag cgcgagcttc gcgggaacaa
     2941 aatcctgaag gaatttcgtc atgtctgatt ttacggcccc cttatttttc cggaaaatgc
     3001 cattatatac caactcgtga gcggacgaat ccagcacaaa atcgatcaaa aggcgggcgt
     3061 tttttcccag agtttcctta ttttttttca taactttccc ttttgatgta tcctatcaac
     3121 ctatcgtgcg actttcaagc cgcgattaca cccgggcggg cgcccggccc acttaatctc
     3181 ctgctcattg tgccgatagg atagaaactt aatggacaag gtgacgtgtg gcacgcagac
     3241 gacgaaaaaa aatcaaaaat ccgtttaaaa ttaaaatcaa ggtcaataaa aagaggctga
     3301 agaaagcgct ctgggttctc agcatcgtta tggcgggtta tctgctcttc ctcggcaccc
     3361 agtatctggt tcgatcggtg cggaaacccc aaccggcctc tcctgtcatt acccgtccga
     3421 ccaccacgac ccggagcccc aaagtgactc aggtgactca ggcgacccag gtggcccagc
     3481 ccgccacttt gtccagaaaa aaaccagtgt cagcggtgac ggcacaagtg ccgccgcccg
     3541 cgcccatcaa gctaacccgc ccttccatcg tcatcgtcat tgatgacatc ggcgctcata
     3601 acaaacttcg tggagaaatg gaacgcctgg gaaacaaagt cacttatgcc attcttcccc
     3661 tgcttcccta ttcccggttt tttagcgaac tcgccacgcg aaaaaacgcg gaagccattc
     3721 tccatcttcc gctggacacg acccaggaca tgatcccggg acgcggcctg atcgtcagca
     3781 ccatgccccg agaacaggct ctcgacatgc tgtcgcgcga cattgcttcc gtcccgggaa
     3841 tcgtcggagt taacaatcac atgggatcaa gagggacggc ggaccgcgag atgatgaccc
     3901 tcatcctgga agaactccat cgccgggggc ttttctttct ggacagctac acgacgaaag
     3961 attccgtcgt cccgtccgtc gcgagacaga tcggaattcc gtttcttcag cggggtgttt
     4021 ttctggacaa tgtcgactcc cccgaggcga ttcgcggcca gatccgggag acggaagccg
     4081 tggcaaaaaa acagggatac gccatcgcga tcggacatta ccggaaaaac acactggacg
     4141 ttctgtttcg cgaaattccg cggctcgaga aaaaaggatt cgcgattatc tcactcagcc
     4201 ggcttttgca gtcccgccgc cggtaacccc gaataaaacc ttcaggcgtc ccctcgggga
     4261 acaagggtcc cgtgaaacgt ttcgtgactt acggaatcga gcgtcaagcg cacgaactcc
     4321 ccgatcaaat ctttctcaca cgcaaaaatg acgcggccgt caaaatccgt tcgcgccaca
     4381 aaactttgtt ctcctttcgg agcccgcgcc tccacaaacc cgctcacctc ccggccgacc
     4441 cgttcgaggt ttttggcttt cgtcacgctc cgctggatcg ccagcagttc attatttcgc
     4501 cgggacttgg tctcaaaagg cacatcgtcc gtcagccgtt ccgccggagt gccgggccgg
     4561 actgaatact tataaataaa ggctgaatca aaccgtatct cctcaatcgc ccgtttcgtc
     4621 gcgagataat cctcttccgt ttctccggga aaccccacga taatatccgt cgtgacagaa
     4681 attccgggaa ccagttcccg aagccggtcc accctggcct tgtaatcggc aaacgtatgc
     4741 aaccgtttca tcctgcgcaa aatcttgtca gaacctgact gaagcggcag gtggaatcgg
     4801 cggctgatct tcgggttctt cgcgatcacc tggaagagtt cctccgtcgc gtcaccggga
     4861 tgcgacgtcg taaacgacaa acgctcaaga ccatcgatcg cgcaaagcag ttccagcaat
     4921 tgaggaaaag aaacattttg agccccggca aggggtacgg cgtccggcgt acgaagcgaa
     4981 acatctccgg gatctgttcc cgcgacaccg cgttccttcg cgccatacga attcacattc
     5041 tgtccgagca gtgtgatcca cttcacgccg tccgccacaa gccgcttcgc ctcccggatc
     5101 acgtcttccg cgggcattga aacctcgcgc ccccgtgttt tcgggacaat gcaaaaagta
     5161 cattccttgt cacaaccggt cataatcgga agccaggcgt gataaggcga gtcgcgtttc
     5221 gtaaattcgc tgtactcgat cgagatcccg tcccgactga tccttgaaac acattcgcga
     5281 gtggcctgaa tccgccggac caaagccggc aattcactga tattccgcgt tccgagaata
     5341 aggtccagat acgggaacct cttgaaaagt ttttcgcgat gctcctccac catacagccc
     5401 atcaaggcga tgatcagttc cggcctctcc tttttttcct tccccaaccg gccaagccgt
     5461 ccccagaccc tctgctccgc gtggtcccgc accgaacagg tgttcataat cacaatatcc
     5521 gctccgcccg aaagacagcc cgccggatcc ggctccactt cagcggaatc ctggagcact
     5581 tcaaatccct cacgctccag aaggcccgcc gccacctgcg aatcgtaaac attcatctga
     5641 catccgaaag tgcggatcag gacttttttc gctacaactt cctgatactt cataattgtg
     5701 acccttccgc tctttgaagt aattttatgc taccaaattg atatttcatc aaaccaatcg
     5761 ggactgcctt aataaaattt ttagcgattt ttaacgcatc tttcctcgaa gtgcccttcc
     5821 ccactcaaat atcaagacca tccctctgaa cttcctctgt cgatcccaaa tagtactcgt
     5881 ctatatcaaa cttttgcccg ctatcctccc aca
//
LOCUS       DAOSIO010000102         5908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_12172_length_5908_cov_4.278661, whole genome shotgun sequence.
ACCESSION   DAOSIO010000102 DAOSIO010000000
VERSION     DAOSIO010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12172_length_5908_cov_4.278661"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1770)
                     /locus_tag="PK590_04800"
     CDS             complement(<1..1770)
                     /locus_tag="PK590_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23949.1"
                     /translation="MPRLSLDGFLSSLRLRPCRFLVPALITLAVLSISGAAWAADPVV
                     YDLMSAQDREVIPSGDAKGNMTATYDEGSRKDVLDASYTLQADSSLVFRAGNFPAGID
                     KINASAIRAGIHLFKPEQTSQITVLVEIRGSKDTQTVPVRLQTGWSRAQAQINWAKLG
                     ELSEIAFVVKPASGVELAKGSLYLSLDFVKGVIPKPKAPSAAISETQAVERKPEAVQT
                     QPAAPIISASANEKKYDVEIFGGAKSSVMLTFDEEIRKDVFDTSYTLVPGSGVRVWNK
                     RLPENLSREEANTLDISLNVLKTDQLGRLVIEAEVKGTDGSKVIPLSVKPGWNLIQKK
                     IPWDETGSLQEVSFILTPKGDSTLSGTVFLGMEFSKTEFLKQYLVPIKLASLLVFAFF
                     AALIARGMNSFFSSRQASPKSVSQNSVSGRIARDFFYGCMIVAITAVTLWIYALGMRD
                     SANEGFCFLGVGLAGLLIAEGLKLKLTGKHLTGGEAFQNVVIVSILAMSSSCLELLQM
                     PTKWEHLLMVNKLTAAVAFVVYQIFNVNTLASQGKHFGGVPAAMVVGTPYLIGWLLLL
                     ENVTMLQTLTYTVTGGLLAAWPVL"
     gene            1959..3173
                     /gene="glgA"
                     /locus_tag="PK590_04805"
     CDS             1959..3173
                     /gene="glgA"
                     /locus_tag="PK590_04805"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HOG23950.1"
                     /translation="MKVLLLTNEYPPFVYGGAGVHVDYLSRELSRLCKVDLRCFGNQK
                     LAKPGLKVTGFDLRKLKTSAPKELRPVWGAAQRCLDFNSAGTDADIVHIHTWYTHLGG
                     ILAKLTYGIPLVLTTHSLEPLRPWKREQLGRGYDFSCWVEKTALEMADAVVAVSEESR
                     RDIFRFFRISREKVHVIHNGIDPEEYRRVRAPEKITRLGIDLKKPYVLFVGRITRQKG
                     IIYLVNAIRHMDPRFQVVLCAGSPDTKEIAREMNARVTAIQKKRPGVIWINQMVDDPT
                     KIALYSHAALFCCPSIYEPFGIINLEAMACEVPVVASAVGGIKEVVVHGKTGALVPLR
                     QNRVSPFEPLLPEKFSKDLAREINRLMSDPASRRRMGKAARKRVVEHFSWRSIAKSYY
                     KLYRSLLASRPG"
     gene            complement(3224..4099)
                     /gene="hflC"
                     /locus_tag="PK590_04810"
     CDS             complement(3224..4099)
                     /gene="hflC"
                     /locus_tag="PK590_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOG23951.1"
                     /translation="MNPAMFLRFWPVVILSVAFLIPSTVFIVDETNQVVITRLGEYKR
                     TLQKPGLKLKIPLFEKANFFEKRILTSGALPAEYLTLDKKRLVADHVTRWKIVDPLLF
                     FKTVRDLAGAKARLDDIVFSEMRRELASHDFSKIISSERENIMETIAGNATKQAAKFG
                     IELIDVRIKRADLPQEVQESVFARMKAERQRIAKRYRSEGEEEAFKIRGETDKSRTIL
                     MAKAYEESQQLRGEGDAAATRVYAEAYGRDAEFYTFVRSLEAYEKSFVDKTTLLFSPD
                     SDVLKHMKENYGRKG"
     gene            complement(4096..5142)
                     /gene="hflK"
                     /locus_tag="PK590_04815"
     CDS             complement(4096..5142)
                     /gene="hflK"
                     /locus_tag="PK590_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HOG23952.1"
                     /translation="MSSENLSPDEQIIYGITNFFKKPGTLPLVGALLIGAWLASGFFI
                     VQPGEQGIIRRFGEKVRTVSAGLNWRMPWPFERADIVNVEQVRRIEVGFRTQSRESAV
                     YRRGGGRSDAMMLTGDENIVEAQIIVQYKISDPVKYLFRLFDPDGVLYTATEVALRSA
                     VGRSTIDDVLTVGRQKVQEETREFLQRLMDDYESGILITEVRLQVVDPPDQVKDAFHE
                     VVRAREDRERLINEAKGYQEDIIPRARGEAQKMLRGAEAYKEERVLKAQGDAARFTAL
                     LEEYQKSKEVTWNRLQIETLEKIYPLATKVIVDPQAGANVLPLLPLGNSSAADLLQGR
                     SDPTLLSRRKEVLS"
     gene            complement(5216..5806)
                     /locus_tag="PK590_04820"
     CDS             complement(5216..5806)
                     /locus_tag="PK590_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HOG23953.1"
                     /translation="MKIFRKLYDWGLKQAESPHAERALFWVAFVESSFFPLPPDVLLI
                     AMVLSARNKWFRYFGICLVGSVIGGVAGYGIGFGAWQAVQGWFFAHVFSEEAFIKVQN
                     LYQHHDFWVIFAAGFTPIPYKIFTIAAGVASIDLTRFILASIVGRGARFILVTGLLYL
                     FGERVRLFIEKYFNLVTIAFFILLAGGFYVLKYCFR"
BASE COUNT         1487 a         1667 c         1400 g         1354 t
ORIGIN      
        1 caacacgggc caggcggcaa gaagaccgcc agtgacggtg taggtaagcg tctgaagcat
       61 cgtcacgttc tcgagaagaa gtagccaccc aatcaggtac ggagtcccga ccaccatcgc
      121 ggcgggcact cccccaaaat gcttgccctg ggaagccagc gtattcacgt taaaaatctg
      181 ataaaccaca aaagcgaccg cggcggtcag cttattgacc atcaaaagat gttcccattt
      241 cgtgggcatc tggagcagtt cgaggcagct cgaggacatg gcaaggattg acacaatcac
      301 aacattctgg aacgcctcac cccccgtcag gtgttttccg gtcaatttaa gcttcaaacc
      361 ttccgcgatc aggagccccg ccaaaccgac tcccaggaag caaaatccct cattcgcgga
      421 atccctcatc cccaaggcat aaatccagag cgtgaccgcc gtgatggcta cgatcatgca
      481 cccgtaaaag aaatcccgcg cgatccgccc agaaacggaa ttttggctaa cagacttggg
      541 agaagcttgc cgggatgaaa aaaaactgtt catccccctc gcgatcaacg cggcaaaaaa
      601 ggcgaacact aaaagggagg caagtttgat cgggacaaga tattgtttta aaaattccgt
      661 cttggaaaac tccatgccca aaaaaaccgt tccgctcaaa gttgaatccc ctttgggagt
      721 cagaatgaag ctgacctcct ggagggaacc cgtctcatcc cacgggattt tcttctggat
      781 caagttccag ccgggtttga cactgagagg gatgaccttg ctaccgtccg tacctttcac
      841 ttcagcctca atcacaagcc ggcctaattg atcggtttta agaacgttaa ggctaatatc
      901 cagggtgttc gcttcttctc ttgaaagatt ttcgggaagt cttttgttcc atacccgaac
      961 ccccgatccg ggaaccaacg tgtagctcgt atcaaaaaca tctttccgga tctcttcatc
     1021 aaacgtcagc atgacggagc ttttggctcc accgaaaatt tccacgtcgt attttttttc
     1081 attcgccgag gcggaaataa tcggagcggc aggttgggtt tgaactgcct cgggtttacg
     1141 ctcaaccgcc tgagtttcag agatggcggc cgaaggcgcc ttgggctttg gaataactcc
     1201 tttaacaaaa tcaagagaaa gataaaggct tcccttggcc aactcaacac cggaagccgg
     1261 cttcacgaca aaagcgatct cgctcaactc cccaagtttg gcccaattga tttgcgcctg
     1321 ggcgcggctc catcctgtct ggagacgtac gggaacggtt tgagtatcct tgctacctct
     1381 aatttccacc aagacggtaa tttgagaggt ttgctcgggc ttgaaaagat ggattcccgc
     1441 ccggatggcg ctagcattga ttttgtcgat tccggcagga aagtttccag ccctgaaaac
     1501 caggcttgaa tcggcctgca aggtataact cgcgtcgagc acgtctttcc gggaaccctc
     1561 atcataggtg gccgtcatgt ttcccttggc atctcccgac ggaattacct ccctgtcctg
     1621 cgcactcatc aagtcataaa cgacgggatc ggcggcccat gcggctccgg aaatcgataa
     1681 aaccgcaagg gttatcagcg ccggcaccaa aaaccggcat ggacgcaaac gaaggcttga
     1741 caagaatcca tctaatgaga gtcgaggcat cttttttgca cctttcttta atcggtgaag
     1801 taactttcga aaaaattccc ttagtttttt ggcgtgccag gaaaattacg tatgaaaata
     1861 tgataacgag atcgaagaat taagaggggg tattatatcc aaaataacta ttttcggaag
     1921 atttgttttt cctgaaaggt tgttatatca tgtcggccat gaaagtcctt cttttaacaa
     1981 acgaatatcc cccctttgtt tacgggggcg cgggcgttca cgtcgattac ttaagccggg
     2041 agctatccag actttgcaag gtcgatctcc gctgttttgg taatcaaaaa ctcgcgaaac
     2101 cggggcttaa agtcaccgga tttgaccttc ggaagctaaa aacctcggcc ccgaaagaat
     2161 tgcggccggt gtggggggcc gcccagcgct gtcttgattt taactcggcc ggaacggacg
     2221 ctgacatcgt ccacatccac acctggtaca cccatctcgg cggaatcctc gcgaaactga
     2281 cctacggaat tccccttgtc ctgaccaccc attctctcga gcccttaaga ccctggaaac
     2341 gggaacagct cggccgggga tatgatttca gttgttgggt cgaaaaaaca gcgcttgaaa
     2401 tggccgacgc ggtcgtcgcg gtgtcggaag aatcgcgtcg ggatatattc cggtttttta
     2461 gaatcagcag ggaaaaggtt catgtcatcc acaacggaat tgaccccgaa gagtatcgcc
     2521 gggtccgggc gccagaaaaa ataacccggc ttgggatcga tttaaaaaaa ccttacgtcc
     2581 tttttgtggg gcgaatcacc cgtcagaagg gcatcatcta tctcgtcaac gccatccgcc
     2641 atatggatcc ccgatttcag gttgtccttt gcgcggggtc tcccgataca aaagagatcg
     2701 cccgggaaat gaacgcgcgc gtcaccgcca tccagaaaaa acggcccggc gttatctgga
     2761 tcaaccaaat ggtggacgat ccgaccaaaa tcgcccttta ttcacacgcc gccctttttt
     2821 gttgcccttc catttatgaa ccgtttggga ttatcaatct cgaagccatg gcctgcgaag
     2881 tccccgtggt cgccagcgcc gtggggggaa ttaaagaagt ggtggtccac ggaaaaaccg
     2941 gagcgcttgt tcctcttcgg caaaatcgcg taagtccttt tgagccactc ctgccggaaa
     3001 aattctcaaa agacttggcg cgcgaaatca accggttgat gagcgatccc gcctcgcgcc
     3061 gacgcatggg aaaagccgct cgaaaacggg tcgtggaaca tttcagctgg cgatccatcg
     3121 cgaaaagcta ttacaaactc taccgctcgc ttctagcgtc ccgcccgggg taaaacccgt
     3181 cgcgcttcct ctggaaaaat ccaggtttta tcccggatgg caatcaacct ttgcgcccgt
     3241 aattttcttt catgtgtttc agaacgtccg aatccggaga aaaaagcaag gtcgttttgt
     3301 ccacaaaact cttttcataa gcctcaaggc tccggacaaa ggtatagaat tccgcgtccc
     3361 ggccataagc ttccgcgtaa acccgggtcg ccgccgcgtc tccctctccg cgtaactgct
     3421 ggctttcttc gtaagctttc gccatgagaa tcgtgcggct cttgtccgtt tcaccgcgaa
     3481 tcttgaacgc ctcctcctcc ccttccgaac gataccgctt cgcgatcctc tgccgctcgg
     3541 ccttcattct cgcgaatacg ctttcctgga cttcctgggg aaggtccgcg cgtttgattc
     3601 tgacgtcgat aagctcaatg ccaaatttcg cggcctgctt cgtggcgttc cctgcgatgg
     3661 tctccatgat gttctcgcgt tcactcgaaa taattttact gaaatcgtga ctcgcaagtt
     3721 cccggcgcat ttcagaaaaa acgatatcat ccaatctcgc cttcgctccc gccagatcgc
     3781 gaacggtttt gaagaaaagc aaggggtcca cgattttcca tcgggtgaca tgatccgcca
     3841 ccaaccgttt tttatcgagc gtcaggtatt cagccggcaa agcgccgctc gtcaggatcc
     3901 ttttctcaaa aaagttcgct ttctcaaaaa gcgggatctt gagtttcagc cccggctttt
     3961 gcaaagtccg cttgtattct ccgaggcggg taataacaac ctggtttgtt tcatccacga
     4021 taaaaaccgt cgacgggata agaaacgcca ccgacaggat cacgaccggc caaaacctta
     4081 aaaacatcgc gggattcatg acagcacctc cttccgcctg gaaagtaatg tgggatcgga
     4141 acgcccctga agcaaatccg cggcagagga gttccccagc ggcaataacg gaagaacgtt
     4201 cgcgccggcc tgagggtcca caatcacttt ggtcgcgaga ggataaattt tttcaagagt
     4261 ttcgatctgg agtcggttcc aggtcacttc ttttgacttc tggtattcct caagcaacgc
     4321 ggtaaacctc gccgcgtccc cctgggcctt caacacacgc tcttctttgt aggcctccgc
     4381 gccccggagc attttttggg cctcaccgcg ggctctcggg ataatatcct cctgatagcc
     4441 tttagcctca ttgataagtc tttccctgtc ttcgcgcgcc cgaaccacct cgtggaacgc
     4501 gtccttcacc tgatccggag gatccaccac ctgaagccgg acctccgtga tcagaatacc
     4561 ggattcgtaa tcatccatga gccgctggag aaactcccgt gtttcctcct gcactttctg
     4621 acggccgacc gtgagaacat catcgatcgt ggaacggccc accgcactgc gaagcgcgac
     4681 ttcggtggcg gtataaagca ctccgtcagg atcaaataac cggaaaagat atttcacggg
     4741 gtcgctgatt ttgtactgaa cgattatctg ggcctcaacg atgttttcgt cgccagtcaa
     4801 catcatcgcg tcagatcgac ctcccccgcg gcggtaaacc gcgctttcgc ggctttgcgt
     4861 cctgaaaccg acctcaatgc ggcgaacctg ctcgacatta acgatgtccg cccgttcaaa
     4921 gggccagggc atcctccagt tcagtcccgc gctcaccgtc cgtacctttt caccgaaacg
     4981 gcggataatc ccctgttcgc cgggttgtac aataaaaaat ccgcttgcga gccacgcccc
     5041 gatcaagagc gcgcccacga ggggaagtgt gcccggtttt ttgaaaaaat ttgtaatgcc
     5101 ataaataatc tgttcgtcag ggcttaaatt ctcggacgac atctttcacc tcccggttaa
     5161 aaatccttaa aaaaacggca ctatcgcgcg ccaacacgac tttttcggtt cctttctaac
     5221 ggaagcaata cttcaggaca taaaaaccgc ccgcaagaag aataaaaaaa gcgatcgtca
     5281 ccagattgaa atatttctca ataaataaac gcacccgctc tccgaaaaga taaagcaacc
     5341 cggtgacaag gatgaacctc gccccccgtc cgacaatcga cgcgaggata aaacgcgtaa
     5401 ggtcaatcga agcgactccc gccgcgatgg tgaatatctt gtaagggatg ggcgtaaatc
     5461 ccgcggcgaa aataacccaa aaatcatggt gctggtaaag attctgaacc ttgatgaaag
     5521 cctcctcgga gaaaacgtgc gcgaagaacc atccctggac ggcctgccag gcgccaaatc
     5581 cgataccgta accggcaacc cctccaatga ccgaccctac gagacaaatc ccaaaatagc
     5641 gaaaccattt attcctcgcg gaaaggacca tcgctatcaa aagcacgtca ggagggagtg
     5701 gaaaaaaaga ggattcaaca aaagcgaccc agaaaagcgc ccgttccgcg tgcggtgatt
     5761 ctgcctgttt taacccccag tcataaagtt tacgaaaaat tttcatctca ccctttctcc
     5821 ttttgtcttt gaggatcatt aaacaggcat tcgtgcaatg ctggtttgcg cctgctcagc
     5881 gacaccggaa ggaaccgcaa tacctatt
//
LOCUS       DAOSIO010000103         5881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_12251_length_5881_cov_4.426021, whole genome shotgun sequence.
ACCESSION   DAOSIO010000103 DAOSIO010000000
VERSION     DAOSIO010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5881
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12251_length_5881_cov_4.426021"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..2106
                     /locus_tag="PK590_04825"
     CDS             4..2106
                     /locus_tag="PK590_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOG23954.1"
                     /translation="MDVTEIFGCNVFNDKTMKERLPKETFKALKKTVQLGLPLSIDVA
                     NVVANVMKDWAIEKGATHYTHWFQPLTGRTAEKHDSFISTTDDGGVIMEFSGKQLIQG
                     EPDASSLPSGGLRSTFEARGYTAWDCTSPAFLKEDASGNVTLCIPTAFCSYTSEALDK
                     KTPLLRSMEAVSHQAVRVLRALGNKSVTRVTSTLGAEQEYFLIDKKYYDQRLDLIVSG
                     RTLFGAASPKGQELEDQYFGAIKDRISTFMRDLDIELWKMGISSKTKHNEVAPSQYEM
                     APIFSTTNIAADHNQLVMETMQKVALRHDLVCLLHEKPFDGLNGSGKHNNWSLSTNDG
                     QNLLEPGKTPNENEQFLIFVSALIMAVDRHADKLRASCGNPGNDLRLGANEAPPTIIS
                     IFMGDELTEILESIAQGKKSKNHGQRVMDIGVDTLPAFPKDNTDRNRTSPFAFTGNKF
                     EFRMVGSSASVSGPNIVINTIVAEALDEIATRLEKAKKSGTSKESAAIVRDTMRKHGR
                     IIFNGNNYSKEWVTEAQKRGLPSIRSGVEALGYMANPVAEKLFGKYEVLSKKEIRSRY
                     EIYLESYVKHVGIEARAMVQMVKRQYLPSVIQFTGRLAGAIHQLKAVSVSAKVQKELL
                     EKVNDLLESANDKLEKLESAIARAHRVETLLKRAEAFRDRVTVTMNDLREDIDTLEGI
                     VPSELWPVPSYADMLFKL"
     gene            2244..2585
                     /locus_tag="PK590_04830"
     CDS             2244..2585
                     /locus_tag="PK590_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase system-associated protein, TIGR04073
                     family"
                     /protein_id="HOG23955.1"
                     /translation="MTIRFLLISLLAGSAVVQAAEIENEGTMLRKLQRGFLNIALSPI
                     EVSNELSKEKRSDTFPPSWVAGLGRGTCFMVGRALTGAYEMVTFPVPYPANYRPILKP
                     EFAWQHLPEKS"
     gene            complement(2783..3922)
                     /gene="lpxB"
                     /locus_tag="PK590_04835"
     CDS             complement(2783..3922)
                     /gene="lpxB"
                     /locus_tag="PK590_04835"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOG23956.1"
                     /translation="MAKKIFLIACEASADLHGAHLIHQLQKLLPESDFCGLGGAQMQT
                     AGVRIFHDLTRISALGLGDVLRNYLTYRKIFHDTIRLIQKEKPDILITIDSPAFNLRL
                     AKKISKTVPIFYFISPQLWAWGKRRIHIIKNHVREMLVILPFEKDFYEKEGVSAKFVG
                     HPLLDSIPELPSASSLRKKIGIPEGKKAIGLFSGSREKEVRRILPLMLKSAELIQKEM
                     PDTVFFLSRSPNVRAEVYLEVLKKSPITCATPDAPFYELVKSMDFALVTSGTATLETA
                     LIGTPFFLLYKTGALTYFLGKRLVKVPYLGLVNLLLGDKVVPEFIQRDAAPEAIAREA
                     KALLSNPEIQMRMKDAFQRVREKLGERGASERAAEEIFRYLSQKQ"
     gene            complement(3927..4865)
                     /locus_tag="PK590_04840"
     CDS             complement(3927..4865)
                     /locus_tag="PK590_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG23957.1"
                     /translation="MTQKLKVGVVGIGHLGKEHARIYSQLAECELTGISDLDPAKEDK
                     ARELKTAFCHDYRDLLDKVQAVSIATPTSTHYQVAKEFLKAGIHVLVEKPITLKLEEA
                     DELIELSQKNHCALQVGHLERHNPGFKRVQEIAKNIRFLEIHRLGPFTGRINDCGVVL
                     DLMIHDLDIVLGLVGSEIASFDALGVNVLTPFEDIANVRLKFKNGAIANITASRLTFE
                     KQRKIRIFQEDAYISLDYGAQTGRIIRKVGDQISQEDVLIEKAEPLKEEIRFFLGGIA
                     SGKSIGKPDTAARNALALAIKIVKVIQENQPKPATK"
     gene            5081..>5881
                     /locus_tag="PK590_04845"
     CDS             5081..>5881
                     /locus_tag="PK590_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOG23958.1"
                     /translation="MDIYKRLLGYIKPHRWRLVVAVLAMFAYSLANSLVSATLYIIVN
                     GLHNKNEVRLDNLPHVPFLSSIAFPVFWIPFVMVGVFFLRGFFDYLSNYQMSNIGIRA
                     IRKVRDDLYRHLVYLSHDFYSRGRTGDFLSRIMNDVGQIQGAITDVIVDLVKQPLVIL
                     FNIPWVFIWGGKYALFAIVIFPLVAIPITILGKRLRKLTKSMQERTGDITSFIGETLS
                     GFHIVKAFCREEKEIGRFEAINRSVFEYFRKTIKITIVQRPLIEIMGAV"
BASE COUNT         1433 a         1371 c         1524 g         1553 t
ORIGIN      
        1 gccattgatg tcacggagat ttttggatgt aatgttttta atgacaaaac gatgaaggaa
       61 agactcccca aagagacttt caaggccctc aagaaaacgg tacagcttgg attgcccctt
      121 tcgatcgatg ttgcgaatgt ggtcgcgaat gtcatgaaag actgggcgat cgagaagggc
      181 gcgacgcatt acacgcactg gtttcaacca ctgaccggaa ggaccgcgga aaagcacgac
      241 tcctttatct cgaccacgga tgacggcggg gtcatcatgg aattttccgg caaacaactt
      301 attcagggtg agccggacgc gtcctcgctc ccgagcgggg gacttcgatc tacgtttgag
      361 gcccgtggct acacagcctg ggactgcact tcgcccgcct ttcttaagga agacgcttcc
      421 ggcaatgtta cgctgtgcat tccgacggcg ttttgctcct acacgagcga ggcgcttgac
      481 aagaaaacac cgctccttcg ttccatggaa gctgtctccc atcaggcggt ccgggttttg
      541 agggcgctcg gcaacaagag tgttacccga gtgacgtcca ccttgggggc tgaacaggaa
      601 tattttttga tcgataaaaa gtattatgat cagcggttgg atctaatagt ttccgggcgc
      661 acgctttttg gcgccgcttc gccaaaggga caggaattgg aggatcagta ttttggcgct
      721 atcaaggaca gaatttcgac gttcatgcgg gatcttgata ttgaactctg gaagatgggg
      781 atttcatcca aaaccaaaca taacgaggtt gccccgtccc aatatgaaat ggcgccgata
      841 ttttcaacga ccaacatcgc ggcggatcac aatcagcttg tcatggaaac catgcagaag
      901 gtcgcgcttc ggcatgatct ggtgtgcctg ttacatgaaa agccttttga cggcctgaat
      961 ggttcgggga aacacaataa ctggtcgctg tccaccaatg acggacaaaa tcttctcgag
     1021 cccgggaaaa caccaaacga aaacgaacag tttctgattt ttgtgagcgc cctgattatg
     1081 gccgtggatc gtcacgcgga caagcttcgg gcgagttgcg gcaatcccgg caacgacctc
     1141 cggctcgggg ccaatgaagc ccctccaacc attatctcta tttttatggg agatgagctt
     1201 acggaaatcc tggaatcgat tgcccagggg aaaaagagta aaaatcatgg gcaaagggtc
     1261 atggatattg gagtggatac cttacctgct tttcccaagg acaatacgga caggaatcgc
     1321 acgtcgccgt tcgcttttac cgggaacaag tttgaatttc gcatggtggg ttcttccgct
     1381 tccgtttcgg ggccgaatat tgtgatcaac acgatcgtgg cagaagcctt ggatgaaatc
     1441 gcgactcggc tcgaaaaggc caaaaaatcc ggtaccagca aagagtcagc cgcgattgtc
     1501 cgggacacga tgagaaaaca cgggcgtatc attttcaacg gtaataatta ttcgaaggaa
     1561 tgggtgacgg aagcccaaaa acgtggtctg ccgagtattc gtagtggagt cgaggcgtta
     1621 ggctacatgg ccaacccggt agcggaaaaa cttttcggta aatacgaagt gctttcgaag
     1681 aaggagatac gttcccgata cgagatctac ctggaaagtt acgtcaagca cgtgggtatt
     1741 gaggcccggg ccatggtcca gatggtgaaa cggcagtatc ttccttcggt gatacagttc
     1801 acggggcgat tggccggagc cattcaccag cttaaggccg tgtcagtttc agcgaaggtt
     1861 cagaaagaat tacttgagaa ggtcaatgat cttcttgagt ccgccaatga caaactagaa
     1921 aagcttgaat ctgccattgc aagggctcat cgggtcgaaa ccctcctcaa gcgagctgag
     1981 gcatttcgtg acagagttac tgtgacgatg aatgatctga gggaggatat agacactctt
     2041 gaggggatcg tgccctcaga gctttggccc gttcccagtt acgcggatat gctcttcaaa
     2101 ctttaacagg tacgcttttt gtcccgaaac ggatcagtca gagagtgaga tatgctttcg
     2161 ccttctaaga tgaaataccc caaagcatca aagaaaaagt tgcctgagct attttgtaat
     2221 gttagagtat ccgcactatg aaaatgacaa ttcgattcct cctgatatct cttttggccg
     2281 gttcggcggt tgttcaggcg gcagagatcg aaaatgaggg aacaatgttg cgtaaacttc
     2341 agcgtggttt tctcaatatc gccctctctc cgattgaagt ttcgaacgaa ctttcgaagg
     2401 aaaaaaggag cgatactttt cctccaagct gggtggcggg attaggccgg ggtacctgtt
     2461 ttatggtggg aagggctctg accggggctt acgaaatggt aactttcccc gtgccatacc
     2521 ccgcgaatta tcggcccatt ctcaagcctg agtttgcctg gcagcactta ccggaaaaat
     2581 cctaatctcg ttcttttccc ggaatttccc ccgagggggc tcctctagaa tcggacgatc
     2641 gtgattaaag attcgaaact tcttcggccc ccgcgttttc aggcccacgc agtcgtcaaa
     2701 tcagatttga tgttgattcc tttattcccg aaatacctgg aaaataaagc acttaacgtt
     2761 cgataggttt ttcccggggg gattattgtt tttgcgataa atatcggaaa atttcctcag
     2821 cggcccgttc gcttgcgcct ctttcaccca gtttttcccg gacccgctgg aaggcgtctt
     2881 tcattctcat ttgaatttcc gggttactaa gcaatgcttt cgcttcgcgc gcgatagctt
     2941 caggggcggc gtcccgctgg ataaactcag ggacgacttt atctcccaag agcaggttca
     3001 cgagaccaag atagggaact ttcacaagcc gctttccgag gaagtaggtt agcgcaccag
     3061 ttttatagag gaggaagaac ggggtaccga tcaacgcggt ttcaagtgtg gcagttccgg
     3121 acgtgaccaa ggcaaaatcc atggatttca caagctcgta gaacggagcg tcgggcgtcg
     3181 cgcaggtgat gggggatttt ttcaagacct cgagatacac ttctgcccga acattgggag
     3241 atcgggacag aaaaaagacg gtatcaggca tttctttctg aatgagttcc gcgctcttga
     3301 gcatgagcgg cagaattcgc ctgacttctt tttcacggga acccgaaaaa aggccaatag
     3361 cctttttgcc ttccggaatg ccaatttttt ttctgagaga tgaagcggag gggagctctg
     3421 gaatagaatc cagcagagga tgtccaacga attttgctga caccccctcc ttttcataaa
     3481 aatctttttc gaaaggtaaa atcacgagca tttcccggac gtggttttta atgatatgga
     3541 tccggcgctt cccccaggcc cagagttgag gcgaaataaa ataaaatatg ggaactgttt
     3601 ttgaaatttt ttttgcgagc cgaagattga aggcgggtga gtcgatggta atcaaaatgt
     3661 cgggtttttc cttctggatt aaccggattg tgtcatgaaa aattttccgg taggtcagat
     3721 agtttcttag tacgtcccct agccccagcg ccgaaatccg tgtcaaatcg tggaaaatcc
     3781 gcacccccgc tgtttgcatc tgagctccac ccagtccaca gaaatccgat tccggaagaa
     3841 gcttttggag ctgatggatc agatgggcgc cgtggagatc ggcagaggct tcacaagcga
     3901 tgaggaagat ttttttagcc atcaaattat tttgttgccg gtttgggctg gttttcctgg
     3961 atgactttga cgattttgat cgcgagagcg agcgcgtttc gtgccgcggt gtcgggtttc
     4021 ccgatgcttt ttccggaggc aatgccgccg aggaaaaagc ggatttcttc cttgaggggt
     4081 tcggcttttt ctatcaaaac atcttcctgg gaaatctgat ccccgacttt ccgtatgatc
     4141 ctgccggttt gggccccgta atcaagggag atgtaagcgt cttcctgaaa aatgcgaatt
     4201 ttgcgctgtt tctcgaaggt cagacgcgat gccgtaatgt ttgcgatagc cccatttttg
     4261 aattttaacc ggacattggc gatatcttca aaaggagtca gaacattgac cccgagcgcg
     4321 tcaaagcttg cgatttcaga tcccacgagt ccgagaacga tatccagatc atggatcata
     4381 agatccaaca ccacgccgca atcgttgatt ctgccggtaa atggacccag acgatgaatt
     4441 tcaagaaagc ggatgttttt cgcgatttcc tggacccgtt taaacccggg attgtggcgc
     4501 tcaaggtgtc cgacttgcag ggcgcaatga tttttctggg aaagttcgat taattcatca
     4561 gcttcttcaa gcttgagggt aataggtttt tcgaccagga catgaatgcc ggccttaagg
     4621 aattctttcg cgacctggta gtgagtggag gttggggtgg cgatgctgac cgcctggact
     4681 ttatcaagaa gatctctgta gtcgtgacag aaagctgttt taagctccct tgccttgtct
     4741 tctttggcgg gatcgaggtc agaaattccc gtcagctcac attctgcgag ctggctgtaa
     4801 attctggcgt gctctttacc gaggtgcccg atcccgacaa cgcctacttt gagtttctgt
     4861 gtcattgaaa tccttttgac cggaggggaa agcttccggt tttaaaccgg atgagtaatc
     4921 aggacaaaac tacggaaaga gctatttaaa tcataacctt gagccgtgtc aatgaatatg
     4981 ttatagtgtc gcctccgcgg tacggatcac aggagacgaa acatgaggca tatagtttgc
     5041 ctcaggatgt gtccctcaca ccgtacctgg cattcaagtc atggatatct acaaaaggtt
     5101 actgggttat atcaaaccgc atcgctggcg ccttgtggtt gccgttctgg cgatgttcgc
     5161 ctattcgttg gccaattctc tggtgtccgc gaccctttac atcattgtca acggccttca
     5221 taacaaaaac gaagttcgcc ttgataacct tccccacgtt ccttttcttt catcgatcgc
     5281 ttttcccgtt ttctggatcc cgtttgtgat ggttggggtg ttctttctgc gcgggttttt
     5341 tgattatcta tccaactacc aaatgtccaa tatcgggatt cgagccattc ggaaggtcag
     5401 ggatgatctt taccggcatc ttgtttattt gtcgcatgat ttttattcca ggggccggac
     5461 cggagatttt ctttccagga ttatgaatga tgtcgggcag atccagggcg ccatcacgga
     5521 tgtgatcgtg gatcttgtga aacagcccct tgtcatcctt tttaatatcc cctgggtttt
     5581 tatttggggg ggcaagtacg cgttgttcgc gatcgtgatt tttccattgg tcgccattcc
     5641 gatcacgatt ctcggaaaac ggctccgcaa actcaccaaa tccatgcaag aacggaccgg
     5701 tgacatcacg tcatttattg gtgagacgct ttcaggtttt catattgtga aggctttttg
     5761 tcgtgaagaa aaagaaattg gccggttcga agcaatcaac cgcagtgttt ttgaatattt
     5821 tcgcaagacg atcaagatta cgattgttca gcggcccctg atcgagatta tgggcgctgt
     5881 g
//
LOCUS       DAOSIO010000104         5878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_12262_length_5878_cov_4.063369, whole genome shotgun sequence.
ACCESSION   DAOSIO010000104 DAOSIO010000000
VERSION     DAOSIO010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12262_length_5878_cov_4.063369"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PK590_04850"
     CDS             complement(<1..250)
                     /locus_tag="PK590_04850"
                     /inference="COORDINATES: protein motif:HMM:NF015414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOG23959.1"
                     /translation="MSEKVVVTRQGYEKMLESLKFLKTTKRREVADQLEKARAFGDLS
                     ENAEYETAKEAKNQLETRIRELEERVAHAKVVELDDIPK"
     gene            complement(366..1511)
                     /locus_tag="PK590_04855"
     CDS             complement(366..1511)
                     /locus_tag="PK590_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HOG23960.1"
                     /translation="MDDPRHFLSKLHSKVVTRERLSLEDGLDLFRSPDVVKIGDLAQS
                     VRRRLHGNTVYYSVNFHLNHTNVCDTRCYFCAFSRDAGDPGAYTLTVDEIETRVREAL
                     ARWDINEVHIVGGHNPALRLDYYAEMIRRLRQIKKERAETGEEKNVPLFIKAFSATEI
                     NDIAKREGLGIREVLEILKQAGLDSMPGGGAEIFSPEVRRKICPRKMSGEEWIAAHRT
                     AHELGIPTNATMLYGHLENDHDCVEHMLKIRALQDETGGFQAFIPLPYCQPRSKSGPA
                     ALSRSVPGETSGYLDLKVLSIARLMLDNVPHVRVHGAATGLKLAQASLSFGVDDIGGT
                     NLDEKIMRESGGANGEKGLSPADLVRYIESAGYTPCLTDSSYAGLQV"
     gene            complement(1521..2600)
                     /gene="ispG"
                     /locus_tag="PK590_04860"
                     /gene_synonym="gcpE"
     CDS             complement(1521..2600)
                     /gene="ispG"
                     /locus_tag="PK590_04860"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOG23961.1"
                     /translation="MKAKIIFPRKKTRSVTVGKFVLGDGQPIRIQSMTKVHTRDVRAV
                     LKQIAQLETAGCEIVRVACATDEDVKAIGKIRPKIHIPLVADIHFNHRFAIEAIEQGV
                     DKLRINPGNIGNEDRVAEVVEKAKKHHIPIRIGVNAGSLEKDLMEKLGPSPEALVESA
                     RRHVAMLERHDFRDIVISLKATDVPTMIQAYRLASKKFEYPLHLGVTEAGTLVRGSVY
                     NAIGVGTLFQEGIGDTVRVSLTADPVEEVKVAKMILESLQLRKGVRVISCPTCGRTEI
                     DTAKLAGEVEARTASFDGPLTIAVMGCVVNGPGEARGADFAITGGKGKGMIYVAGKQE
                     RVVPEKKLVNELLKEIHKRSANRER"
     gene            complement(2590..3960)
                     /gene="thiC"
                     /locus_tag="PK590_04865"
     CDS             complement(2590..3960)
                     /gene="thiC"
                     /locus_tag="PK590_04865"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOG23962.1"
                     /translation="MTLRDEYLKKRGSDAVKTQMHYARQGRVTEEMIGVAKRESVSPQ
                     FIRDEIAAGRLVIPANIFHAGLEPIGIGINVSCKINANIGNSALGSNIAEELEKVKVA
                     VRYGADTIMDLSTGPLLNETRAAVIQASTVPVGTVPIYQALERVGGKPEALTPDLFWD
                     VVEFQAKQGVDFMTVHAGVRLKHVELVKSRLTGIVSRGGAILARWCQEHGKENFLYTD
                     YDRLLEICRKYDVTISLGDGMRPGCLADASDEAQFMELDTLGELTLQAWKQDVQVMVE
                     GPGHVPMNQIPMNIERQIKVCHGAPFYVLGPLVTDIAPGYDHITSAIGAAIAAQAGAA
                     MLCYVTPCEHLRLPNLEDVKKGVIAYKIAAHAADIARQRPGARDRDDRMARARFAFDW
                     EKQFELSLDPENARKVHDEGLPKGTTHREEYCSMCGEGFCAMRLSKGLGPNPFKKCPA
                     VSDNES"
     gene            complement(4128..5165)
                     /locus_tag="PK590_04870"
     CDS             complement(4128..5165)
                     /locus_tag="PK590_04870"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23963.1"
                     /translation="MINENDLLLLSLLRQGVPFVNRPFDAIGEQMGLDGADVILQIER
                     MRKEGYLSAIRPIWDHRFFGYQGAWAAMKFSEQSLEQKLEIIGQHPGVIYMAQRDHEV
                     NGWFYITAPGGHDLEAHVRILEKKTEAEKTLFLPIQRILKGAGEMRVFAGGVPEQVNQ
                     TAGSIKRTGETKLTLQEIRLIRLLQEDFLLTDEPFRRMAETLAIPESAMLHLLQGLFQ
                     KGYLKRIGISGGSKPDSQQDRFLVVWQIPEEKINFCVERFADFPSVISAGLRPAAPEF
                     PYTVYTTVESKELETLELLILKIADKIGHWPNRVILTVREFRKTAIRYFPRELDAWWA
                     RWKTLADPAFI"
     gene            complement(5396..>5878)
                     /locus_tag="PK590_04875"
     CDS             complement(5396..>5878)
                     /locus_tag="PK590_04875"
                     /inference="COORDINATES: protein motif:HMM:NF025097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HOG23964.1"
                     /translation="LDGTETVIGNDNLLMAYAHVAHDCVIHNGCTLANNGTLAGHVIV
                     EDKAILGGLSAVHQFVRIGTLSIIGGCSKVVQDIPPFMMVDGHPAKAFGINSVGLDRA
                     GGYTAKDKADLKKAFRIIFKSRLGLKHAMKEIRAQIPSTPPIETLVAFLEKSERGICG
                     "
BASE COUNT         1397 a         1706 c         1305 g         1470 t
ORIGIN      
        1 ccttgggaat atcatccagc tccacaacct tggcatgggc aacccgttcc tcgagttccc
       61 ggattcgagt ttcaagttgg tttttggctt ctttcgccgt ctcgtattcg gcattttcac
      121 tcagatcccc gaaagctctg gccttttcca gctgatccgc cacttcgcgg cgtttcgtcg
      181 ttttaagaaa cttgagagac tcgagcattt tttcataacc ctgtcttgtg acaacaactt
      241 tttcggacat gttaaactcc ttccgttaac ccccgctgaa tttcaacaaa actactgccc
      301 aaattttaaa acaacaaatt aaaaaccatt gtcctcttta acctcaatcc tcgccacaac
      361 ctttttcaga cttgcaaacc cgcgtaggaa ctatcggtaa ggcagggcgt atacccagcg
      421 ctctcgatgt atcgaacaag atcggcagga cttaaaccct tctccccatt cgcgcctcct
      481 gattcgcgca taattttctc atccagattg gtcccaccga tatcatcgac accaaaggat
      541 agggaggctt gagcaagttt aagacctgtt gccgctccat ggactctgac gtgagggacg
      601 ttatccagca taagccttgc gatggaaagc actttcaaat cgagataacc gctcgtttca
      661 cccggaacag aacgcgacaa tgccgcaggc ccagattttg aacgaggttg acaatagggc
      721 aaggggataa acgcctgaaa accacccgtc tcatcctgga gagctcgtat tttcaacatg
      781 tgttccacgc aatcgtgatc gttttcaaga tgcccataga gcatggtagc attcgtgggt
      841 atcccaagct catgagccgt ccggtgagcc gcgatccact cctcgcccga catctttcga
      901 ggacatattt tcctccgaac ctccggagaa aatatttccg cgccaccgcc gggcatactg
      961 tctaatccgg cctgtttcaa tatttcaaga acctcccgaa tgcccaaccc ttcacgtttt
     1021 gctatgtcat taatctccgt cgcgctaaat gccttaataa acaagggaac atttttttct
     1081 tctccggttt cggcccgttc tttctttatt tgccggaggc ggcgtatcat ttcagcgtaa
     1141 tagtccagcc tgagtgccgg gttgtgccct cccacaatat gcacttcatt aatatcccat
     1201 cgcgcgagcg cctcacgaac ccgcgtttcg atctcatcca cggtcagggt gtaggcgcct
     1261 gggtctccgg cgtcccggga aaaagcgcag aaatagcacc gcgtgtcgca aacattcgtg
     1321 tgattaagat gaaaattgac ggaataataa accgtattcc cgtgaagccg tcgacgaacc
     1381 gactgcgcga ggtccccgat tttgacaaca tccgggcttc gaaacaaatc gagcccgtct
     1441 tctagggaca atcgttcacg ggtcacgacc ttggaatgaa gcttggaaag aaaatgacgc
     1501 gggtcatcca tgaaacaatt ttaccgttcg cggttagcgc tacgtttatg gatctctttc
     1561 aaaagttcat taaccagttt tttttcagga acaacccgtt cctgttttcc ggcgacatag
     1621 atcatgcctt tccccttgcc tcccgtaatc gcgaaatcag cccctcgagc ttctccaggc
     1681 ccattaacga cacatcccat caccgcgatc gtgagcgggc catcgaacga cgcggtccgg
     1741 gcttctactt cacccgcaag ttttgccgtg tcaatttccg tgcggccaca agtcgggcac
     1801 gagatcactc tgacgccctt tcgaagttgc agggattcaa gaatcatttt cgcgactttt
     1861 acctcttcaa cgggatccgc cgtcagggaa acgcgaaccg tgtcgccaat accttcctga
     1921 aaaagcgttc ccacgccgat ggcattataa acacttcccc ggacaagagt ccccgcctca
     1981 gtgacgccga gatgtagagg atactcaaat tttttactgg cgagccggta ggcctggatc
     2041 atggtaggga catccgtcgc tttcagcgaa atcacaatgt ctcggaaatc atgtcgttcc
     2101 agcatcgcca catgccgccg cgcgctttct accagggctt caggcgacgg ccccaatttt
     2161 tccatcaggt ctttttccag tgatccggcg ttgacaccga tccggatcgg gatgtgatgc
     2221 ttttttgcct tctccacgac ctcggccact cgatcttcgt ttccgatatt ccccggattg
     2281 atccggagct tgtccacgcc ctgctcaatc gcttcgatcg cgaatcggtg gttgaagtgg
     2341 atatcagcca cgagcgggat gtgaatttta ggacgaattt tcccgatggc tttcacgtct
     2401 tcatccgtcg cgcaagcgac ccttacgatc tcacatccag cggtctcaag ttgcgcgatc
     2461 tgtttaagca ccgcccggac atcgcgggta tgaaccttcg tcatggattg aatccgaatc
     2521 ggctgaccgt cgcccaaaac aaactttccg accgtgacag accttgtttt ttttcgggga
     2581 aaaatgatct tagctttcat tgtcagaaac cgctggacac tttttaaatg ggttcggacc
     2641 caaccccttc gagagcctca tcgcgcaaaa tccctcccca cacatggagc aatattcctc
     2701 tcgatgggtt gtccctttgg gaagaccttc atcgtgaacc ttgcgcgcgt tttcaggatc
     2761 aagagaaagt tcaaattgct tttcccaatc aaacgcaaat cgcgccctcg ccatccggtc
     2821 gtcccgatcg cgcgctcccg gccgctgacg ggcgatatca gccgcgtggg ccgcgatttt
     2881 ataagcaatc actcccttct tcacatcctc cagattggga agcctgagat gctcacacgg
     2941 ggtcacataa caaagcatcg ccgcgcccgc ctgggccgcg atcgcggcgc caatcgcgga
     3001 agtaatatgg tcatagcccg gcgcgatgtc cgtcacgagc ggcccaagca cgtaaaaagg
     3061 ggctccatga caaaccttga tctgcctttc aatgttcatc gggatctggt tcatcggaac
     3121 atgtcccggg ccttccacca taacctgaac atcctgtttc caggcctgta acgtcaattc
     3181 accaagcgtg tcgagttcca tgaactgcgc ctcgtcactc gcgtcggcca gacatcccgg
     3241 cctcatacca tcacccaggc tgatcgtaac atcatatttt ctgcaaattt ccagcaggcg
     3301 gtcataatcc gtgtaaagaa aattctcttt cccgtgttcc tggcaccacc tcgcgagaat
     3361 agcgccgcca cggcttacga tcccggtcag cctgcttttc acaagctcaa cgtgcttcaa
     3421 acgaactccc gcgtgaacgg tcatgaaatc aactccctgt ttcgcctgaa actcgaccac
     3481 gtcccagaaa aggtctggcg taagcgcctc cggcttccca cccacacgct ccagcgcctg
     3541 gtagatgggg actgtcccca ccggaaccgt gctcgcctga atcaccgccg cccgggtttc
     3601 attcagtagg ggccccgtcg aaagatccat aatcgtgtcc gcgccatacc tgaccgcaac
     3661 tttgaccttt tcaagttcct ccgcgatgtt cgagccgagc gctgaattgc caatattcgc
     3721 gttgatcttg caggaaacat taattcctat tccgatcggt tcaagccccg cgtgaaaaat
     3781 attcgcgggg atcacaagtc tcccggcggc aatttcatca cggataaatt gcggcgacac
     3841 cgattcccgc ttcgcgaccc cgatcatttc ttccgtcacg cgcccttgcc gggcataatg
     3901 catttgggtt ttcacggcat cacttcctct tttttttaaa tactcatctc gcaaggtcat
     3961 agcttccttt ttcgcgcaac gcaccacaat atcgtcttcg agcgttgtaa cactttacgc
     4021 tcagtaaata agggctcggg gagtttcctc cgctcctcat ccttcacctt cgctctcaac
     4081 agatctgaga ccctccgggt cgtggacagg ataacttcct caatggatca aataaatgcc
     4141 ggatccgcga gtgttttcca tcgcgcccac caagcatcca gttccctggg aaaatatcga
     4201 atcgccgtct tcctgaactc acgcaccgtc agaatgaccc ggttcggcca atgtccgatc
     4261 ttgtcagcaa ttttaaggat taacagctct aaagtttcaa gttctttgct ttccaccgtg
     4321 gtatatacgg tgtagggaaa ttccggcgcg gcgggtctca gacccgcgga aataacgctt
     4381 gggaaatccg cgaaacgctc cacgcaaaaa tttatttttt cttccgggat ttgccaaaca
     4441 acaagaaaac gatcctgctg ggaatcgggt tttgaacctc cggaaatgcc gatccgtttc
     4501 agataccctt tttgaaaaag tccttgtaac agatgaagca tcgcggattc tggtatcgcc
     4561 aaagtttccg ccatcctgcg aaacggctca tccgttaaca aaaaatcttc ctgcaaaagg
     4621 cgtatcagcc ggatttcctg aagtgtcagc ttcgtttccc cggtccgttt tattgacccg
     4681 gccgtttgat taacctgttc cgggacgccc cccgcaaaaa cccgcatttc tccggcgcct
     4741 ttcaggatac gctggatcgg aagaaataaa gttttttcag cttcagtttt tttctcaaga
     4801 attcggacat gcgcttcaag gtcatgtccc ccaggcgccg tgatataaaa ccagccattg
     4861 acctcatggt ctctctgagc catgtaaatc acaccgggat gctgcccgat aatttccagt
     4921 ttttgttcta aactttgctc tgaaaatttc atggccgccc aagcgccctg atagccaaag
     4981 aaacggtgat cccaaatcgg ccgaatcgcc gaaaggtatc cttcttttct catccgttca
     5041 atttgaagaa tcacatcggc gccatccagc cccatttgtt cgccaatcgc gtcaaacgga
     5101 cgattgacaa aagggacccc ttgtcggaga agacttaaaa gaagaaggtc gttttcgtta
     5161 atcatggcaa gtgcgtagta tatagaactt tatacgctgt aaccaaaaaa tatttctctg
     5221 aagaattcca aaaaacacat cgaatagtaa atcaggattt taaccagtca caggtaaaag
     5281 ctctaaaaat acttttggga gctgaagatg gaatggcaag atatttcaag taaaacacaa
     5341 aacctcaagt cgacaacccc accggacagt cggttccgaa acgatgacgg cttacttaac
     5401 cgcaaattcc ccgttcggat ttttcaagaa aagccacgag cgtctcaatg ggcggagtgg
     5461 aaggaatctg cgcgcggatt tccttcatcg cgtgtttaag tcccaggcga gatttgaaga
     5521 tgatcctgaa agctttttta aggtccgcct tgtccttcgc cgtatatccc ccggcacgat
     5581 ccagtccgac ggagttaatt ccaaaggcct tcgcgggatg cccgtccacc atcatgaatg
     5641 gggggatatc ctgcaccact ttggaacatc cgccgataat cgaaagggtt ccgatcctga
     5701 caaactgatg aaccgcgcta agtccgccta gaatagcctt gtcttcaaca atgacatgcc
     5761 ctgcaagcgt cccgttgttg gcgagcgtgc acccgttatg gataacacag tcatgcgcga
     5821 cgtgcgcgta ggccatgaga agattgtcat ttccgatcac ggtttcggta ccgtcgag
//
LOCUS       DAOSIO010000105         5792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_12490_length_5792_cov_3.910755, whole genome shotgun sequence.
ACCESSION   DAOSIO010000105 DAOSIO010000000
VERSION     DAOSIO010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12490_length_5792_cov_3.910755"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1923)
                     /gene="recG"
                     /locus_tag="PK590_04880"
     CDS             complement(<1..1923)
                     /gene="recG"
                     /locus_tag="PK590_04880"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOG23965.1"
                     /translation="MNSLSGSHSRYPAGTSDIRYVKGIGPRRAEIFRRLGIHSLKDLF
                     YLFPRRYEDRSRFHTLASFPLGEPATCRGEILEIKFKPIRRMPILEVLLGDDTGTLRA
                     IFFNQPYLRRTFETGQWVVFHGKLENYEGRLQLPNPEFEIMDRSDENSPHVGRIVPIY
                     PLTEGLFQRSLRMIMKDAIEKHLNETISDYLPPALRNDMGFVPLDRALHDIHFPATLE
                     DQSRARQRLVFDDFFSFTLSLLIRVKTLRTQFQSPAIRPGPGCFEVFKKALPFELTGS
                     QEHAMREIEKDVGALVPMNRLLQGDVGSGKTVVAAFAFFLTARAGFQSAFLVPTEILA
                     EQHFRTLSRLLTPLNITVELLTKSVAEDDRKRINATLKRGDLSVLVGTHALLQEDVVF
                     HNLAMVTIDEQHKFGVHQRGQLFQRSPRPHQLVMTATPIPRTLGLTLFADLDVSLMRE
                     MPKGRKPIATYWISRAKQAEVYRHVARRLSKGEQAYIVFPVIEESEKVDQRAAKKEHS
                     RLQKEVFPEFKVGLVHGKLDRSEREAIMRAFGRREIHVLVATSVIEVGVDQPNATVMI
                     IENADRFGLAQLHQLRGRIGRGSLESECFLFGEPSTEEGKKRLRLLTKLNDGFLIAEE
                     DLKLRGDGGQLEMVLLN"
     gene            complement(1992..2462)
                     /gene="secG"
                     /locus_tag="PK590_04885"
     CDS             complement(1992..2462)
                     /gene="secG"
                     /locus_tag="PK590_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOG23966.1"
                     /translation="MSLVIVILHVLVCLVLILVILLQAGRGQGLTGASFGGGNVQSIL
                     GTKASSFLTKATSVCAVLFLLTCIGLNAVEIQKSRSLFHAPASQAPLDVDQIRQALDK
                     IKQSDSAAQESATATADAVAAETESVKETSDLAEKSAENLLAEAVQKTDDAVSN"
     gene            complement(2459..3244)
                     /gene="tpiA"
                     /locus_tag="PK590_04890"
     CDS             complement(2459..3244)
                     /gene="tpiA"
                     /locus_tag="PK590_04890"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOG23967.1"
                     /translation="MKKKPLIAGNWKMYKTASEAVTLVQTLKAAVHPVMNVEIVICPP
                     FIALSEVSKVLTDTPIELGAQNMYSAPEGAFTGEISPMMLKDVRCRFVILGHSERRQY
                     FKEDNILIHEKVKTALKYSLVPIVCVGETLEEREGKAHFDVVKTQFDECLGSLEKDDI
                     AKVVIAYEPVWAIGTGKTATPDQAEQMHSYIRRLLNERFGKELGVKVPILYGGSVKPD
                     NARSLMEKPNIDGALVGGASLKAETFAQIVREARMAYESGVNT"
     gene            complement(3241..4539)
                     /locus_tag="PK590_04895"
     CDS             complement(3241..4539)
                     /locus_tag="PK590_04895"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014311932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOG23968.1"
                     /translation="MEKKTVKDIEIRGKRVLMRVDFNVPMKDGVVQDETRIKAALPTI
                     KYVLDQGAKSLVLMSHLGDPQKDTKKAREKAEKDGKPFDEAKYLAGKHKMASVAERLA
                     KLLGKEVKLAPSCMGPETDAMIKAISQGGILMLENTRFHKEEKAKEPAERDIMGKTLA
                     QYGDLFVNDAFGSAHRAHASTESVAKFLPAVAGFLMEKELLYLEEKVVKNPARPFVAL
                     IGGAKISSKIAVLESLLSKVDRLIVGGGMAYTFLKAKGLSIGNSLIEEDMIDTAHKIL
                     QKAYESHIYLYLPIDHIVTKEFAADAEPKHVARGNIGKEQMGMDIGPMTIEKFRGALQ
                     GAKTVFWNGPVGVFEFPNFAKGTMEIARAISELPDCVTVVGGGDSVAAVNQSGVADKI
                     SHISTGGGASLELVEGKTLPGVAVLLDKEQNKKGCCLSRS"
     gene            complement(4639..5712)
                     /gene="gap"
                     /locus_tag="PK590_04900"
     CDS             complement(4639..5712)
                     /gene="gap"
                     /locus_tag="PK590_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOG23969.1"
                     /translation="MGIKVGINGFGRIGRLVFQAICDQGLLGKEIDVVAVVDISTDAD
                     YFAYQIKYDSVHGHFKHDVKTEKSDPSLPEADTLVVNGHKTKCVMATKTPDQLPWKAL
                     GVDYVVESTGLFTDSEKAKLHLTAGAKKVIISAPGKGDVKFLLMGVNENEYDSAKHNV
                     VSNASCTTNCLAPLVHVLLKEGIGIETGLMTTIHSYTATQKTVDGPSKKDWRGGRAAA
                     INIIPSTTGAAKAVGEVLPAVKGKLTGMSFRVSTPNVSVVDLTFRTLKDTSIEEIDSL
                     LKKASQTYLKGILGVTTEELVSTDFIHDNRSSIYDSLATLQNNLKGEKRFFKLISWYD
                     NEWGYSNRVIDLLRFMAKKDCVK"
BASE COUNT         1376 a         1640 c         1377 g         1399 t
ORIGIN      
        1 gttgagcagc accatttcca gttggccgcc gtcgccccgc agtttcaggt cttcttccgc
       61 gataagaaat ccgtcgttta gcttggtaag aagccggagc cttttttttc cttcctccgt
      121 cgagggttcc ccaaacaaaa aacattccga ctccagactt ccccggccaa tccggccccg
      181 aagctgatga agctgagcca gtccaaatcg atcggcattc tctataatca tgaccgtcgc
      241 gtttggctgg tccactccga cctcaatgac cgacgtcgct acaagcacat ggatttctct
      301 ccggccaaaa gcccgcataa tcgcctccct ctccgatcga tccagtttac cgtggaccag
      361 gccaaccttg aactcaggaa acacctcttt ttgaagacgc gaatgttctt ttttggccgc
      421 ccgctgatcc actttttccg attcctcaat gacgggaaac acaatgtagg cctgctcccc
      481 ttttgaaaga cgccgggcaa catgacgata gacctccgcc tgtttggcgc gggaaatcca
      541 gtaggtcgcg atgggttttc gtccttttgg catttcccgc ataaggctga catcgagatc
      601 ggcaaaaagt gtaagcccaa gcgtcctcgg gatcggcgtc gcggtcatga ccagctgatg
      661 cgggcgcggt gatcgctgaa acagttgccc tcgctgatgc acgccaaatt tgtgctgttc
      721 gtcaatcgtt accatcgcga ggttatgaaa aacaacgtcc tcctgaagca gggcgtgagt
      781 tcccaccagc acggaaagat caccccgttt gagcgtggcg ttgatcctct tacgatcgtc
      841 ctccgcgaca cttttcgtga gaagttcgac cgtgatattc agaggagtta acaaacggga
      901 tagagtccgg aaatgctgtt cggccaaaat ttcagtcggg accagaaacg ccgactgaaa
      961 gccggcccgg gccgtaagga aaaaagcgaa cgccgcgaca accgtttttc ccgatcccac
     1021 gtctccctgc agaagtcggt tcatggggac gagagccccc acgtcttttt cgatctcccg
     1081 catggcgtgt tcctgacttc ccgtcagttc aaacggaagc gcctttttaa atacctcgaa
     1141 gcatcccggc cccgggcgaa tggccgggga ttgaaactgg gtccgcaacg ttttgacccg
     1201 aatcagaaga ctcaaagtaa aagaaaagaa atcgtcaaaa acaagtctct gccgcgcccg
     1261 ggattggtcc tcaagggtgg ccggaaaatg tatgtcgtga agagcccgat ccagagggac
     1321 gaaacccata tcatttcgca acgcgggcgg cagatagtct gaaatcgttt cattcaggtg
     1381 tttttcaatc gcgtctttca tgatcatccg gagggatcgc tgaaaaagtc cctcggtcaa
     1441 cggataaatc ggaacaattc tcccaacatg aggggaattc tcatcagatc gatccatgat
     1501 ttcaaattca ggattaggca actgaagacg tccctcataa ttttcaagtt ttccgtggaa
     1561 aacgacccac tgccctgtct caaaggttcg gcgcaaataa ggctgattaa aaaaaatcgc
     1621 ccggagggtt ccggtatcat cacccaaaag cacctccaga atcggcattc tgcgaatcgg
     1681 tttaaatttg atttccaaga tctcgccgcg gcatgttgcc ggctctccaa gggggaaaga
     1741 tgccaacgta tgaaaccggg atcggtcttc ataacgtcgg ggaaaaaggt aaaaaaggtc
     1801 tttgagggaa tggattccaa gtcgacggaa tatttcggct cgcctgggcc cgatcccttt
     1861 cacatagcgg atgtccgatg tccccgctgg ataacgcgaa tgagatcctg aaagagagtt
     1921 cattggctcc gtaaaccaaa aaggacagcc cggcaaacaa cggcggggct gtccttttga
     1981 gaaaaatctg tttagttaga gacggcgtcg tccgtctttt gaacagcttc agccaaaaga
     2041 ttttcggcag atttctcggc caaatcggac gtttctttaa cactttcggt ttccgccgcg
     2101 accgcgtccg cggtagcagt cgccgattcc tgagcggcag aatccgattg cttgatcttg
     2161 tccagggcct gacgtatctg gtcaacgtcc agaggcgcct ggctggcggg agcgtgaaaa
     2221 agagaacgac ttttctgaat ttccaccgcg ttaagcccga tacaggtcaa gagaaacaac
     2281 accgcgcaaa cgctggttgc cttggtgaga aatgaggacg ccttggttcc taaaatagac
     2341 tggacgtttc ccccgccaaa cgacgcgcct gttaacccct gaccgcgtcc ggcctggaga
     2401 agaatgacca gaatcagaac cagacacacc aacacgtgca aaataactat gacaagactc
     2461 atgtgttcac ccccgattca tatgccatcc gggcttcccg aacaatctgg gcaaacgttt
     2521 ccgccttgag ggacgcgccc ccgacaagcg cgccatcgat attcggtttt tccatcagcg
     2581 atcgcgcatt gtccggtttg acactcccgc cgtaaaggat cggcactttg acgcccagct
     2641 cttttccgaa acgctcgttc aggagccggc gaatatagga atgcatctgt tcggcctggt
     2701 ccggggtcgc tgtctttccg gtcccgatcg cccaaaccgg ttcgtacgcg atgacgacct
     2761 tcgcgatgtc atctttttca agacttccga ggcattcgtc aaactgggtt ttcaccacat
     2821 caaagtgggc tttgccttcc cgctcctcca gtgtttcccc aacacaaaca atcggaacaa
     2881 gactgtactt gagcgctgtt ttgacctttt catgaatcag gatgttatct tccttgaaat
     2941 attgcctccg ttcggaatgc cccagaatga caaagcgaca acgaacatct ttcagcatca
     3001 tcggcgaaat ttcacccgta aaggcccctt ccggcgcgga atacatattc tgcgcgccca
     3061 gctcaatcgg cgtatcagtc agaacctttg aaacttccga aagcgcgatg aacggcgggc
     3121 agatcacgat ttcaacattc atcaccgggt gaacggcggc tttcaacgtt tgaacgagcg
     3181 tgacggcctc agaagccgtc ttgtacattt tccagtttcc ggcaattaaa ggttttttct
     3241 tcatgagcgg cttaaacagc accctttctt gttttgttct ttatcgagaa gcaccgccac
     3301 cccgggaagt gtctttcctt ccacgagttc cagtgacgcg ccccctccgg ttgaaatatg
     3361 agaaatttta tcggcaactc ccgactggtt gaccgccgcg accgaatcgc caccgccaac
     3421 caccgtcacg caatccggaa gttccgaaat cgcccgggcg atctccatag tccctttcgc
     3481 gaaattcggg aactcaaaaa caccgactgg cccgttccag aaaacggtct tggcgccctg
     3541 caacgcgccc ctgaattttt cgatcgtcat ggggccaata tccatcccca tctgctcctt
     3601 tccgatgtta ccacgcgcga catgcttcgg ctccgcgtca gccgcgaatt ctttcgtcac
     3661 aatatgatcg atgggcaggt aaagataaat atgagactca tacgcctttt gcaaaatctt
     3721 atgcgccgtg tcgatcatgt cctcttcgat cagcgaattc ccgatcgaaa gacctttggc
     3781 cttcaggaac gtataggcca ttccccctcc cacaataagc cgatcgactt tggaaagcag
     3841 gctctcaagc accgcgattt ttgaagaaat cttggcgccc ccgatcaagg caacgaaagg
     3901 ccgggccgga tttttcacga ccttttcctc aagatacagc agttctttct ccatcaaaaa
     3961 acccgccaca gccggtaaaa atttcgccac cgattccgtc gaagcgtgcg cccgatgcgc
     4021 ggaaccaaac gcgtcattca cgaagagatc cccgtattgg gcgagggtct tccccataat
     4081 gtcccgttcc gcgggctctt tcgctttttc ttccttgtgg aaccgggtgt tttccagcat
     4141 caggattccc ccctgggaaa tcgcttttat catggcgtcc gtttcaggcc ccatgcagga
     4201 cggggcaagc ttgacttcct taccgagtaa tttcgcgagg cgctccgcca ccgaagccat
     4261 tttatgcttg cccgcgaggt attttgcttc atcaaacggt ttcccgtcct tctccgcttt
     4321 ctcgcgcgcc ttcttcgtat ccttttgggg atctccgagg tgggacatca gaaccagact
     4381 tttcgcgccc tgatcgagga catacttgat ggtgggcaaa gcggccttga tacgcgtctc
     4441 atcttgaacc acgccgtctt tcatggggac gttaaagtcc acccgcatta aaacacgttt
     4501 accgcgaatt tcgatgtcct tgactgtttt cttttccatg atggtccttg tcgatggtca
     4561 ttgtcagatt tacagggacg attcacgccg agacagcgac gaaccgtccc tgagtccagc
     4621 ttgcctgaag tgtcgggttt acttaacgca gtctttcttc gccatgaacc ggagaagatc
     4681 aatcactcga ttggaataac cccattcgtt gtcgtaccag gaaataagct taaaaaaacg
     4741 cttttcccct ttcaggttat tctgaagcgt cgcgagcgaa tcataaattg aagaacggtt
     4801 gtcatgaata aaatccgtcg acaccagttc ctcggttgtc acgcccaaga tgcccttcag
     4861 gtaagtttgc gaagcctttt tcagaagaga gtcaatttcc tcaatcgaag tgtctttgag
     4921 ggttcggaag gtcaggtcaa cgaccgaaac gttcggtgtg gaaacacgaa acgacatacc
     4981 ggtcagcttc cccttgacgg caggtaacac ttccccgacc gctttggccg cgcccgtcgt
     5041 tgagggaata atgttgattg ccgccgcgcg gccgcctcgc cagtcttttt tcgaagggcc
     5101 atcgaccgtt ttttgcgtcg cggtgtaaga atggatcgtg gtcataagac ccgtttcaat
     5161 cccgatacct tctttcagga gcacatggac gagcggggcc aaacaattgg tcgtacagga
     5221 agcattcgac acgacattat gcttggcgct gtcatattca ttttcattaa cgcccatcag
     5281 caaaaatttg acgtcgcctt ttccgggcgc gctgatgatc actttcttcg cgccagccgt
     5341 caggtgcaac ttcgcctttt cagaatccgt aaaaaggccc gttgattcaa cgacatagtc
     5401 aactccaagc gctttccagg gaagttgatc gggggtcttg gtcgccatca cgcatttcgt
     5461 tttgtgccca ttaaccacca gcgtatccgc ttcgggaagt gaaggatcac ttttctcggt
     5521 cttgacatcg tgcttgaaat gcccatgaac cgaatcatat ttaatctgat aagcgaaata
     5581 atccgcgtcc gtcgaaatat caaccacggc cacaacgtca atttccttgc cgagaagccc
     5641 ctgatcgcag atcgcctgaa aaaccaaacg tccgatacgc ccgaaaccgt taataccgac
     5701 cttgataccc atatgtgtaa tcctcctgga ttagtttcgc tacttggtca ccctggacat
     5761 gtgcctgaag gaatgacaaa agaaagcggt aa
//
LOCUS       DAOSIO010000106         5745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_12626_length_5745_cov_4.352548, whole genome shotgun sequence.
ACCESSION   DAOSIO010000106 DAOSIO010000000
VERSION     DAOSIO010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12626_length_5745_cov_4.352548"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..519
                     /gene="gatC"
                     /locus_tag="PK590_04905"
     CDS             235..519
                     /gene="gatC"
                     /locus_tag="PK590_04905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOG23970.1"
                     /translation="MNVDIDKIAQLARIHLTPEEKPGLEKDLEAILGYIGKLDRLDTE
                     KITPTSHVLNLENVCRKDTPGLSDAAEKAIEHAPCAEGRFFKVPKIVQRD"
     gene            589..2016
                     /gene="gatA"
                     /locus_tag="PK590_04910"
     CDS             589..2016
                     /gene="gatA"
                     /locus_tag="PK590_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOG23971.1"
                     /translation="MDLTKLTLKEAVEFSKENGVQPILDAFQKRAGLLNSRINAFLRC
                     DSLLEKMSIPGTSALLAGVPISIKDNICIEGREVTCASKIMTKHVPPYDATVIEKLKS
                     AGATIFHQCNMDEFALGSSCETSAFGACRNPWDFNCVPGGSSGGSAAAVAADMTVAAI
                     GSDSGGSIRQPAALCGVVGVKPTYGRVSRYGLIAFGSSFDQIGPITKTVEDAAILMNV
                     ISGHDPKDSTSAPDDVPDFTQSLKRQVKGLRIGLPTEYFIEGIDPEVEKAVRQAGEIY
                     KRLGAEIKIISLPHTEYSVAVYYIVAVAEASSNLGRFDGVEYGLRVPSKDLREMYFET
                     RNQGFGQEAKRRILLGTFVLSAGYYDAFYLKGQKVRTLIRQDFEKAFKEVDVILSPTS
                     PTPAFKIGERTEDPIAMYLSDIFTIPANLAGIPAMSVPCGFTAGGLPIGLQLMARPFD
                     EATLFRAAYTFEQETGVYKKKPKLD"
     gene            2177..3286
                     /locus_tag="PK590_04915"
     CDS             2177..3286
                     /locus_tag="PK590_04915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG23972.1"
                     /translation="MEPEKAIHEIGRIYDAITDKSVLIGFFLQTVIQFIPSQKAYLFL
                     AGPDKRLWLEEAILGPKEASPQLLQSVQQAFDKGKPSVQKGMLFLPMIARNMSIGVAC
                     FERDGDFTPKELEMGFDFVSEFSGALKNILLFEENIRMERLAAIGQTMAMLVHEIKNI
                     LQLAKFSDEMIRMGIHEKNEKFLTRGIEKLGKTLKDMDGFIGEITSLSKDYQLEPEVV
                     SIPALIEELKNDLSERAEAVSVKLEFSTEEDFPEVQGDSRALYRAILNLVKNAFEAFK
                     NKPDACIKVFARLVDSERYEVSVQDNGAGMPDEVKVRLFETFFSTKGKRGTGLGLMVV
                     SRTVNMHRGTVAVESQPGVGTKFTLTLPRIFKKTE"
     gene            complement(3353..4663)
                     /locus_tag="PK590_04920"
     CDS             complement(3353..4663)
                     /locus_tag="PK590_04920"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG23973.1"
                     /translation="MKKTSRPPTQKTNLTTKRCPADKVRNLQKILALNSILNSTLELD
                     PLLAIIMKTSAEVMKAEVASLLLIDSATKELVFRVALGGKGSALEEKFRVKMGEGIAG
                     SVAKNGKPLVVNDVRNDPRFAKRFDRSTGFITKAILCVPLKAKNKIIGVLQAINPIGR
                     KGFCSSDLDLFETFAHQAAVAIENAKLHAEMVRQETAKQTLKIAHEIQQNFLPDLKNR
                     NYGLEIDAKSVPAFDVGGDFYDAVTLDNDRLGIILGDVSGKGVPAALYMVRAMSEYRF
                     LAPSAKDPAELLTRLNKKLSEQSFFGMFITMVCLMVDKTTGTIQYASAGHPPILLRRA
                     ANSTTELLKGTQCVPLGLVPDAPFFLNTIKLEKGDALFLYTDGVTEARNRLGKEYTIE
                     GLTRCVKVGAKSAEAYLEQICADVRSFSADTDPHDDVTALALVI"
     gene            complement(4660..5370)
                     /locus_tag="PK590_04925"
     CDS             complement(4660..5370)
                     /locus_tag="PK590_04925"
                     /inference="COORDINATES: protein motif:HMM:NF023178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HOG23974.1"
                     /translation="MFLFEKLILAHLIADFVLQFEELYQLKVRRLLGHLLHVFIHGTV
                     MIILAFPYLDNPLVFLYIIFLVFEHLLQDLIKYRLTQKFPTKQFIYYTLDQILHFVVL
                     GFVLFFPVASETRPFPGPVPLNELYQDPVITRFFIFFILLTFAANYTLNSFYRSYVKG
                     SRPLHWITSPEMAITILERSVIASVMIFAPSLSWILLSLTVGALRIPLKSLRHFKDFL
                     ISAGYTLALTAVFMVLAL"
     gene            5465..>5745
                     /locus_tag="PK590_04930"
     CDS             5465..>5745
                     /locus_tag="PK590_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG23975.1"
                     /translation="MKKRSILIVDDEVDFPRLARMVLTEKGGFEVGVCHRGEQAIDMI
                     REWKPDLLLLDIVMPNVDGTDIAAALRNDPELCSVPIIFFTSLMTPREAA"
BASE COUNT         1501 a         1244 c         1542 g         1458 t
ORIGIN      
        1 caacgattcg gccaatgatc gctctatggt aagatttaat atgagtcaac tcgccggcag
       61 gactgccatt tgacactctc ttcgccttcg ggcagaatgt caggcaatgt atccttttct
      121 aggctactta aaatggtcga gtggttcgaa aagattgtat cgggttgctt cgagaaaaat
      181 cggtaagagc ggaagatccg gaatggtttc ccatgaattt ggaatggagg caaaatgaac
      241 gtcgatattg ataagatcgc gcaacttgcg aggattcacc ttacgcccga agaaaaaccc
      301 gggcttgaaa aagacctcga agccattctg ggctatatcg gcaaacttga caggcttgat
      361 accgaaaaaa tcacgccgac aagccacgtt ctcaatctgg aaaatgtgtg ccggaaggat
      421 acccccggtc tttcggatgc cgccgagaag gccatcgagc atgcgccatg cgcggagggg
      481 cgttttttta aagttcccaa aattgtgcag cgagattgag gttggacttt cgccaggtat
      541 cgggcggtca gcgaaattcc cggaaacgcc cgggatgaga aggaagaaat ggatttaaca
      601 aaacttaccc ttaaagaagc agttgaattc tcaaaagaga atggtgtcca gccgattctg
      661 gatgcctttc agaagcgcgc gggattactg aactccagga tcaacgcttt tttacgatgc
      721 gattcactcc tggagaagat gtcgattccc ggcacttcag ccctcttggc aggagttccc
      781 atttctatca aggacaatat ttgcattgag ggccgggaag tgacttgcgc ctcaaaaatt
      841 atgacgaagc atgttccgcc ttatgatgct acggtgattg aaaaactcaa atcagcggga
      901 gccaccattt tccatcagtg caacatggat gagttcgcgc ttggatcttc gtgtgagacg
      961 tcagccttcg gagcttgccg taacccatgg gatttcaatt gtgttcccgg cgggtcaagc
     1021 ggcggatccg cggcggccgt tgcggctgac atgaccgtgg cggcgattgg ttcggacagc
     1081 ggaggttcca ttcgtcagcc tgccgcgctg tgcggagtag tgggcgtgaa gccgacctac
     1141 ggccgcgtat cacgttatgg gctcatcgcc tttggctcaa gttttgatca gattggtccc
     1201 attacaaaaa cagttgagga cgcggcgatc ttgatgaacg tgattagcgg acatgatccc
     1261 aaagattcca catcagcgcc tgatgatgtg ccagatttta ctcaatctct caaacggcaa
     1321 gtcaagggac tcaggatcgg actacccacg gaatatttta ttgagggcat tgatcccgaa
     1381 gttgaaaagg ccgtacgtca ggcgggggaa atttacaagc gacttggcgc ggagatcaaa
     1441 atcatttccc tcccgcatac ggaatattcc gtcgcggttt attacatcgt ggccgtggcg
     1501 gaggctagct ccaatctcgg gcgttttgac ggagttgaat acggactccg ggtaccttcc
     1561 aaggatcttc gggagatgta ttttgaaact cgcaaccaag gttttggcca ggaggcgaag
     1621 agacgtattt tgcttggaac gttcgtcctc tccgcgggtt attacgacgc tttttatctg
     1681 aaagggcaga aggtccgtac cctgatccgg caggatttcg aaaaggcttt caaggaagtt
     1741 gacgtgatcc tttctccgac gtctcccacg ccagcgttta aaataggaga aagaactgaa
     1801 gacccgattg ccatgtatct atccgatatt tttaccatac cagccaatct tgccggaatt
     1861 ccagcaatgt cagttccctg tggttttacg gctggtggac tgccgatcgg tcttcagctg
     1921 atggcgcgtc cctttgacga agcaaccctt ttccgtgccg cctatacttt tgagcaggag
     1981 accggtgtct acaagaagaa accgaagtta gattaaaccg atagattttt caacggggtt
     2041 tgacaatggt acgggatggt gaacgactaa cttcgcgaag atgggcttga agatgagttt
     2101 gataatgtcc atggatctgg atcgaacggg tgagtgtgct tgaaatccaa cagtgtattg
     2161 attaaactcc tttcgtatgg aacctgaaaa ggcgattcac gagattggcc gaatctatga
     2221 tgccattacc gacaaaagtg ttctgatcgg gttttttctt cagacggtca tccagtttat
     2281 tccttcccaa aaggcatacc tttttcttgc gggtcccgat aagcggctgt ggctcgaaga
     2341 ggcgattttg ggccccaaag aggcttcgcc ccaactatta caatcggttc aacaagcctt
     2401 tgacaagggg aagccctctg tccaaaaagg aatgcttttt cttcccatga ttgcccgcaa
     2461 catgtccatt ggagtagcgt gttttgaacg ggacggggat tttactccga aggaattgga
     2521 aatggggttt gattttgttt ctgaattttc aggagctctc aaaaatattt tgctttttga
     2581 agaaaacatc aggatggaac ggctcgcggc catcggacaa acgatggcaa tgctggttca
     2641 tgaaattaaa aacatccttc agctcgcaaa attctccgac gaaatgatcc ggatggggat
     2701 ccacgagaaa aacgaaaaat ttcttactag ggggattgaa aaactcggga aaaccctcaa
     2761 ggacatggat ggctttatag gggagattac gagcctgagt aaagactacc agctcgaacc
     2821 ggaagttgtg agcattcccg cgctgatcga agaattaaaa aatgatcttt cggaaagggc
     2881 ggaagctgtt tcggtaaaac tggaattttc cacggaagaa gatttccctg aagtgcaggg
     2941 agatagtagg gctctctatc gggcgattct taatcttgtt aaaaatgcct ttgaagcttt
     3001 taagaataag cccgacgctt gtatcaaggt ttttgcgagg ttggtggatt cggaaagata
     3061 tgaagtttcg gttcaggata atggcgctgg aatgccggac gaagtgaagg ttcggctttt
     3121 tgaaacgttt ttttccacga aagggaaacg agggacggga ctgggtctga tggttgtttc
     3181 tcgcacagtg aatatgcacc gggggacagt ggccgtggaa agtcagccgg gtgtcgggac
     3241 aaaattcacc ctgacacttc cccgaatttt taaaaagaca gaataaattt aagcagattt
     3301 gcgatcccaa ccgtaaacga ttgagactga ttctgaaaat ttctgttggg tattaaatga
     3361 ctagggccaa cgctgtgacg tcgtcgtgtg ggtctgtgtc ggctgaaaaa gagcgaacgt
     3421 ccgcgcagat ttgctcaaga taagcttccg ctgatttggc tcctacctta acacagcgag
     3481 tcaggccttc aattgtatac tctttcccga gcctgtttcg cgcttccgta acgccatccg
     3541 tgtaaaggaa aagggcgtca cctttttcaa gtttgatcgt gttaagaaaa aacggagcgt
     3601 ctggtacgag tcccagaggg acacactgag ttcctttgag gagctcggtg gtactgtttg
     3661 cggcacgcct cagtaaaatg gggggatgtc cagcggacgc atactggatt gtaccagtgg
     3721 ttttatccac catgaggcag accatagtga tgaacatacc gaagaaactt tgctcggaca
     3781 gttttttgtt gaggcgcgta agaagttcag ccggatcttt ggcggaggga gccagaaaac
     3841 gatattctga catcgctctg accatgtaca aagcagccgg gacacccttt cctgatacat
     3901 cgcccaggat aattcccagg cgatcattat cgagagtgac ggcatcataa aagtcgcctc
     3961 cgacatcgaa cgcgggcaca cttttggcgt cgatctccaa tccatagttt ctgtttttta
     4021 gatccggaag aaaattttgc tgaatttcat gggcgatttt taatgtctgt ttcgcagttt
     4081 cctgtctgac catctcggca tggagtttcg cgttttcgat cgcgacggcc gcctggtgcg
     4141 cgaaggtttc aaaaagatcc aggtcagacg aacaaaagcc tttgcggccg atgggattaa
     4201 tggcctgaag caccccgatg atcttgttct tggctttcag aggcacgcaa aggattgctt
     4261 tcgtgatgaa tccggtggat cggtcaaaac gcttcgcgaa acggggatcg ttccgcacat
     4321 cattcacgac cagaggtttt ccgtttttgg cgaccgagcc ggcaatgcct tctcccattt
     4381 taacgcgaaa tttttcttcc agggccgaac ccttgcctcc taatgccacg cggaagacaa
     4441 gttccttggt tgccgaatca atcaaaagca gggaagcgac ctctgctttc atgacttcag
     4501 cggaggtttt catgatgatt gcgagtaatg gatccagctc aagcgtggaa ttcagaatgc
     4561 tgttgagagc taggatcttt tggaggtttc gtaccttgtc agctggacaa cgttttgtgg
     4621 tcaaatttgt tttttgggtg gggggacggg aggtcttttt cataacgcca gtaccatgaa
     4681 aacggctgtc agcgccaggg tgtatccggc gctaataagg aaatccttaa agtggcgtaa
     4741 acttttaaga ggaattcgga gagcaccaac cgtcaaggaa agcaatatcc aggagaggct
     4801 cggagcgaaa atcatgaccg acgcgattac gcttcgttcg aggattgtga ttgccatctc
     4861 aggagacgtg atccagtgta acggccgtga gcctttcaca tagctccggt agaatgaatt
     4921 caacgtatag tttgcggcga aggtaagtaa tatgaagaaa ataaagaaac gggtgatgac
     4981 cggatcctga taaagctcat ttaaaggaac cggtcccggg aaaggacggg tttcgcttgc
     5041 cacgggaaag aacaaaacga agccaagaac gacgaagtgg agtatctgat ccagcgtata
     5101 gtaaatgaat tgtttggtcg gaaatttttg ggtcagacgg tatttgatca aatcctgaag
     5161 aaggtgttca aagacgagga atataatgta aagaaaaacc agcgggttgt ccaggtaggg
     5221 gaaggcgagg atgatcatga ctgttccgtg gatgaaaacg tggagcagat gccctaaaag
     5281 gcgtctgacc ttaagctgat acagttcttc aaactgaaga acgaaatcgg caatcaaatg
     5341 cgccagtatg agtttttcga ataaaaacat atccgtctcc ctgataaagc cgtattatag
     5401 cttcaaaaaa gaggccttgg gtatggtaaa atacgaacca agcgtagcat aggagtgtct
     5461 gacgatgaaa aagcggtcca ttcttattgt ggatgatgaa gtggatttcc cccggcttgc
     5521 gaggatggtg ttgactgaga aggggggatt tgaagtgggg gtttgccatc gtggtgagca
     5581 ggcgattgac atgattcggg aatggaaacc ggatcttctt ttgttggata ttgttatgcc
     5641 caatgtggat gggaccgata ttgcggccgc cctcagaaat gatccggagc tctgttcggt
     5701 tccgatcatt ttttttacat cgcttatgac gccgcgagaa gccgc
//
LOCUS       DAOSIO010000107         5713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_12709_length_5713_cov_4.102156, whole genome shotgun sequence.
ACCESSION   DAOSIO010000107 DAOSIO010000000
VERSION     DAOSIO010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12709_length_5713_cov_4.102156"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..1387)
                     /locus_tag="PK590_04935"
     CDS             complement(137..1387)
                     /locus_tag="PK590_04935"
                     /inference="COORDINATES: protein motif:HMM:NF014541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23976.1"
                     /translation="MTLPPDLKQKCLVLYVGTRKLVALLADLRESQPRVLHKEEVLFP
                     DGFEKGYVCNLQNAALSLEKIMGAILPPEAWEHVSVYVVLGNSGFKFYRFSSCEYYTP
                     DKRTISSHEVQSVVQQTRSVATLPLSESILQAVPESFLVNDMAAVRNPVGLEAERLGV
                     TLQLYTMDFQNFRNIVKAFEAIDVDVCGYFPKALTVAESVLTDAEKQEGVLIIDIGDD
                     AAQLVLWKNGYLAGIRSLSAGGSELTRCIARDYHIEERDADKVKFQFATLDESERVSE
                     ELVPLVTRNEKVQQSVHRKEFLDRFFAHAKDWMSGILQESKKFSEGEGVRYPHMVFTG
                     GSVMMDGFLEWMQKEFGIEGRIGVSRQIEAPQEIIRDPSLAPVTGMLRWIMTAKQENE
                     ALFTSRSFMARMIAAVRAWFTNYF"
     gene            complement(1384..2196)
                     /locus_tag="PK590_04940"
     CDS             complement(1384..2196)
                     /locus_tag="PK590_04940"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOG23977.1"
                     /translation="MGRKKNKKRGKKNGFWASVSMFIIQITKHVIPLMLIVAAFLFGA
                     VGVWKVLLADTHLRISEIRVVPSDVLTSAHIKVLEDKLIGKNIMGLDLRKVESEIELG
                     PGAKGVRVVRELPSTIRIEIEKRKPAANMLFKARGAYGIVADDGIIIATSNVMEPAWV
                     LIENFSEPIQEPKIGAKIQNKGFPEALKFLKKYEKHDLSRKERITRVMLDPYGNVTIR
                     LGEGPDFQLGRRPTEKFPMLSKAMYLFKTEAREGVDYMDLQFDRIAVKRKQS"
     gene            complement(2200..3153)
                     /locus_tag="PK590_04945"
     CDS             complement(2200..3153)
                     /locus_tag="PK590_04945"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOG23978.1"
                     /translation="MTKVLTSTSKFSQLQKIRVGVVCGGTSAERNISIRSGRGVLKAL
                     VEFGIQAERLDPATKTFWKKTENFDILFLVLHGNGGEDGSIQAILERRGIPFTGSGSK
                     ACRKSFNKLVTKKYLRKWGIPTPDYAVLSQKDWKKRAILFPTPYFVKPLDGGSSQGVF
                     PVEDFKKSAETIRKSLARHQRLLIEKRIYGRELTCGILAGRALPVVEVRPRRSFYDYK
                     AKYTRGMTEYLVPAPIPRCAASKVMKLTEKVCGAMGLKDFARVDFMLDEQNKPYVLEV
                     NSIPGMTELSLLPKAARAVGISYHEVCLTILAKSLKRFRGR"
     gene            complement(3258..4652)
                     /gene="murC"
                     /locus_tag="PK590_04950"
     CDS             complement(3258..4652)
                     /gene="murC"
                     /locus_tag="PK590_04950"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOG23979.1"
                     /translation="MTIVHEDIVVGCREAYLIGIGGAGMSGLARILKHLGLSVRGSDR
                     KESSVTRNLRNDGIGVRIGQEEIGLGSADLVIYSSAISKNHLELVEARRSGRKVYHRA
                     EVLASLMNHADTALGVLGTHGKTTTSAMLSFVLSRIGADPTCFVGSDMINFGTNVVVG
                     GRKYWVSEVDESDSSHEYYAPHYAVITNLEPEHMEHYQSWENLQGSFRRFLAQLHRPG
                     LVAYFGNDPVLCQIVKESGRPSVRFGFTEDCDFSAQNVRPLPYGSEFDLWEEGFFSRS
                     VRLSVPGRHNVANALAAISLLSQIGFDMDLVADILSEFKGTKRRMEVKWQSAQFIVLD
                     DYAHHPTEVKAVLKALRETGKYVRVIFQPHRFSRTQHLYRAFAHAFDDADEVLLTDIY
                     SAGEDNPYGISVDRIAQEVVASGHSNVRIVHREQIIPYLQAHPLVDGVMVFMGAGDIG
                     ELANEFVGGIGMHA"
     gene            complement(4636..>5713)
                     /locus_tag="PK590_04955"
     CDS             complement(4636..>5713)
                     /locus_tag="PK590_04955"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOG23980.1"
                     /translation="TGGHLFPAQAFAESFRDRHFGSRLELVTSARAKKLVARFPKGLF
                     DGVHYLPDFGLPNRLFSFQSLKPLFLAPYLFTRSFFHLRSFRPDLCVGFGSFVSYPGM
                     RLARLLGIPTLLHEQNQYAGKATRWLVRHADAVAESFEGTNFLAATRRLYTTGLPLRK
                     LILHGEFHGTRQSDQIRILVMGGSQGARGLNRAVLDCWSTLRDEEKNKFAVTHITGDK
                     EAESVAERYGELGVRSRVYPFYENMAELFRGSELAVTRAGANTLFELAFYGLPAFVVP
                     FPHAGGHQRLNAQAFARHGALEFHDEDELLGGWLADKMRGVLENRGQLESMSVAMKNL
                     ARPKAGDQLVEISLELLKKHDDRS"
BASE COUNT         1509 a         1655 c         1187 g         1362 t
ORIGIN      
        1 tgaaagatgt tcttcgtgaa gtggaagtct ttttgaagtt atatcggtcg tcatcttgtc
       61 atcccttaaa aacgctggaa gagccgcgat tatactggaa aaaatctttt ttaatagaac
      121 gtttttagtc tatggctcaa aaataattcg taaaccacgc gcgaaccgcc gcgatcatac
      181 gcgccataaa acttcgggaa gtaaagaggg cttcattctc ctgcttggcg gtcatgatcc
      241 agcgtaacat gccggtcacc ggagcaagcg aagggtcacg aataatttcc tggggggctt
      301 caatctgacg gctaacacca atccgccctt caataccgaa ctccttctgc atccactcga
      361 gaaacccgtc catcatcacc gaaccgcccg taaagaccat gtgaggatag cgaacgccct
      421 ccccctccga aaatttttta gattcctgga ggattccact catccagtct ttggcatgcg
      481 cgaaaaaacg atccagaaac tccttacgat gaaccgattg ctgaaccttt tcattgcgcg
      541 tgacaagcgg caccaactcc tcactcaccc gttccgattc atccagcgtc gcaaactgaa
      601 atttcacctt gtcggcgtcc cgttcctcga tgtgataatc tctggcaatg caacgcgtca
      661 gttccgaacc cccggccgaa agactgcgaa ttcccgcgag atacccgttt ttccacagaa
      721 caagctgggc cgcgtcatcc cctatatcga tgatcaaaac accttcctgt ttttccgcgt
      781 cagtcaacac agactccgcc acggtcagag ccttagggaa atagccgcac acgtccacgt
      841 caatcgcctc gaaagcttta acaatatttc gaaaattctg gaaatccatg gtgtaaagct
      901 gaagcgtcac tccgagacgc tcggcttcca gccccaccgg gtttcgtacg gccgccatat
      961 cattgaccaa aaatgattct ggcaccgctt gcaggattga ttcgcttaaa ggaagcgtcg
     1021 caacactccg ggtctgttgc acgactgact gcacttcatg agaacttatc gtccttttat
     1081 cgggcgtgta atattcacag cttgaaaaac gataaaattt aaaaccgctg tttcccaaca
     1141 ccacataaac agacacatgt tcccaggctt ctggcggcaa aatagcgccc attattttct
     1201 caagggagag cgctgcgttt tgaagattac acacgtagcc tttttcaaaa ccatccggaa
     1261 aaagcacttc ttctttgtgt aaaacccggg gctgtgattc acgcaaatcc gcaagcaacg
     1321 ccaccagctt gcgagtcccg acataaagaa caaggcattt ctgcttaaga tcaggaggca
     1381 aagtcacgat tgtttcctct tcacggcaat cctgtcaaac tggagatcca tatagtcaac
     1441 accctcccga gcctctgttt taaaaagata catcgctttg gacagcatgg gaaacttctc
     1501 cgtgggtcgc cttcccagct gaaaatccgg cccttcacca agccggatgg taacgttccc
     1561 gtacggatcc agcatgacgc gcgtaatccg ttctttccgc gaaagatcat gtttttcata
     1621 ttttttcaaa aatttcagcg cctcggggaa tcctttgttc tgaatcttgg ccccgatttt
     1681 cggctcttga atcggttccg aaaaattctc gatcagcacc cacgctggtt ccatcacatt
     1741 actggtcgcg ataatgatgc catcgtccgc aacaattccg taagcgcccc ttgctttaaa
     1801 aagcatattc gccgcgggtt ttcttttttc aatttcaatc cggatcgtcg acggcaattc
     1861 ccgaacaacg cggacacctt tagcgcccgg ccccagttca atttcagact cgacctttct
     1921 aagatcaagc cccatgatat tctttccgat cagtttatct tccagaacct tgatgtgagc
     1981 ggacgttaaa acgtccgagg gcacaacccg tatctcggaa attctgagat gagtgtcagc
     2041 cagtagcacc ttccagacac caacagctcc aaataaaaaa gccgccacaa tcaacatcaa
     2101 tggaataaca tgcttggtta tctgaatgat aaacatcgag acactggccc aaaagccgtt
     2161 tttctttcct cgttttttat ttttttttcg ccccataaac tatcttcctc gaaaacgctt
     2221 caatgattta gccaagatcg ttaaacaaac ctcatggtaa ctaattccca cggcccgcgc
     2281 cgccttgggg agcaagctca actccgtcat gcctgggata ctattcactt caagcacata
     2341 cggtttattt tgctcatcca gcataaaatc gacccgggca aaatctttca accccattgc
     2401 tccgcacact ttttccgtaa gcttcatcac ttttgacgcg gcacaacgcg ggatcggcgc
     2461 cggcaccaaa tattcggtca tgccacgagt atacttcgcc ttgtagtcat aaaaggaccg
     2521 ccggggacga acctcaacga ctggcaaagc ccggccagcg agaataccac aggtcagttc
     2581 ccgtccataa attctctttt caattaagag ccgttgatgc cgggccaagg acttgcgtat
     2641 tgtttcggcg gatttcttga aatcttcaac cggaaatact ccctggctcg acccaccatc
     2701 caagggcttc acgaagtagg gcgtcggaaa aaggatagcc cgtttcttcc agtctttttg
     2761 ggaaagcacg gcataatccg gtgtcggtat cccccacttt cgcaaatact ttttcgtgac
     2821 cagcttgtta aaacttttcc ggcacgcctt cgaaccggat cccgtaaaag gaattcctcg
     2881 gcgttctaaa atcgcctgga tcgacccgtc ctcccccccg tttccatgca atactaaaaa
     2941 aagaatatca aaattctctg tttttttcca aaaggttttg gtcgcgggat ccaaccgttc
     3001 cgcctgtatc ccgaactcaa ctagggcttt taaaacccca cgccccgaac gaattgaaat
     3061 gtttcgctcc gcggaggttc cgccacagac aactcccacc ctaatttttt gaagctggga
     3121 gaacttcgaa gttgaggtca acaccttggt cattctgttt cactttcaga ttagtcacaa
     3181 acaggacggt aaaaatatta acgatacact cttgaattct tacaaaaaaa aaagaaaaac
     3241 tccggaaaac ctaacattca cgcatgcatt ccaatccctc caacaaattc attcgccagt
     3301 tcgccaatgt cccctgcccc cataaacacc atcacaccgt cgaccagcgg atgcgcctga
     3361 agataaggaa tgatctgttc tctgtgcaca atgcgaacgt ttgagtggcc cgaagcaacg
     3421 acttcctggg caatcctgtc gacgctgatt ccgtacgggt tatcttcacc cgcgctataa
     3481 atatctgtca gtaaaacttc atccgcatcg tcaaacgcgt gggcaaaagc ccggtaaaga
     3541 tgttgcgtgc ggctgaagcg gtggggctga aaaatgacac gcacgtattt tcccgtctcc
     3601 cgaagcgcct tgagaaccgc tttgacttcg gtcggatgat gcgcatagtc atcgagaaca
     3661 ataaactggg ccgattgcca ctttacttcc atgcgccttt tcgtcccttt aaactccgac
     3721 agaatatccg cgacaagatc catatcaaaa cctatttgag aaagtaaact aatggcggca
     3781 agcgcattcg ccacgttatg acgtccgggc accgaaagac gtaccgaacg actgaagaac
     3841 ccctcctccc aaagatcaaa ctcggaccca tagggaagcg gccgtacatt ctgggcgcta
     3901 aaatcacaat cttccgtaaa tccaaatcga acagaaggtc gcccgctctc ttttacaatc
     3961 tgacacagta ccgggtcatt accgaagtac gcgacaagac ccgggcggtg aagttgcgca
     4021 agaaaccgtc ggaaagatcc ctgaagattt tcccagctct gataatgctc catatgttcc
     4081 ggctcaagat tggtgatcac ggcataatga ggcgcgtaat attcgtgaga actatcactt
     4141 tcatccactt ccgaaaccca gtatttcctc cccccgacaa caacgttcgt tccaaaatta
     4201 atcatgtcgc ttccgacaaa acacgtcggg tccgcgccga ttcgcgacag aacaaaagac
     4261 agcatcgcac tggtcgtcgt tttaccatgc gtccccagga cacccaacgc ggtatcggca
     4321 tggttcatca gcgacgcgag cacttccgcg cgatgataaa ctttccgtcc cgaacgtcgg
     4381 gcctcaacca gttcaaggtg attcttgctg atcgccgatg aataaatcac aaggtcagcc
     4441 gaccccagac ctatttcctc ttgtccgatc cggacaccaa tgccgtcatt tcgaagattt
     4501 cgggtcacgg acgattcctt acggtccgaa cctctcaccg aaagtccgag gtgcttgagg
     4561 atccgtgcca gcccgctcat ccccgcccct ccaataccaa tcaggtaagc ttcccgacac
     4621 ccaacgacaa tatcttcatg aacgatcgtc atgttttttt aataactcca gggagatctc
     4681 tacgagctga tcccccgctt tgggccgcgc aagatttttc atcgcaaccg acattgattc
     4741 aagttggccc ctgttctcta aaacaccgcg cattttatca gccaaccagc cgcccagcag
     4801 ttcatcttca tcgtgaaatt ctaaggctcc gtgccgcgcg aaagcctggg cattcagcct
     4861 ttggtgcccg cccgcgtggg gaaaaggaac gacaaaggcc gggagcccat aaaaagccag
     4921 ctcaaaaagc gtattcgctc cggcgcgcgt tacggccaac tcagaacccc gaaaaagctc
     4981 ggccatgttt tcatagaacg gataaaccct gctacgaact cctaattccc cgtaccgctc
     5041 cgcgacgctt tctgcttctt tatcacccgt aatatgagtt acggcaaatt tatttttttc
     5101 ttcatccctc agcgtcgacc aacaatcgag tacggccctg tttaggccgc gcgctccctg
     5161 cgatcctccc atcacgagaa tccgaatttg atccgattgt cttgttccgt gaaactcgcc
     5221 atgcagaata agttttctca agggtaaccc tgtcgtataa agacggcgcg tggccgccaa
     5281 aaaattcgtc ccctcgaaac tttcagccac cgcgtccgcg tgccgtacga gccatcgcgt
     5341 cgctttcccc gcgtattgat tttgctcgtg caaaagcgtc ggaatcccca aaagtcttgc
     5401 cagtctcatc ccgggatatg aaacaaaact gccaaacccc acacaaagat caggcctaaa
     5461 actcctcaag tgaaaaaacg atcgagtaaa aagatagggc gcgaggaaaa ggggtttcaa
     5521 cgattgaaac gaaaacaacc gattcggcag accaaaatca ggaagataat gaacgccgtc
     5581 aaaaagacct ttcgggaaac gggcgacaag ttttttcgct ctcgcgctgg tcacaagttc
     5641 cagtcgactt ccgaaatgcc ggtcccgaaa cgattccgcg aacgcctgcg caggaaacaa
     5701 gtgtcccccg gtc
//
LOCUS       DAOSIO010000108         5699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_12750_length_5699_cov_3.908575, whole genome shotgun sequence.
ACCESSION   DAOSIO010000108 DAOSIO010000000
VERSION     DAOSIO010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12750_length_5699_cov_3.908575"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PK590_04960"
     CDS             <1..527
                     /locus_tag="PK590_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOG23981.1"
                     /translation="KAALSEAKGDLKTAQDILRKKGLDIAKKKSTREAREGQIVSYIH
                     SGGKLGVILEVNCETDFVAKNETFQSFCRDLAMQIAAAHPLFVSPESIPSEIMEREKA
                     IFLDQVKDKPAPVQAKIIEGKLAKRYEEICLLNQKFIKDDSKTVQDILTAMIAKIGEN
                     ILVRRFVRFEVGGN"
     gene            603..1316
                     /gene="pyrH"
                     /locus_tag="PK590_04965"
     CDS             603..1316
                     /gene="pyrH"
                     /locus_tag="PK590_04965"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOG23982.1"
                     /translation="MKKAVYKRIVLKLSGEALEGSRDFGIDPEVVKSIARQIKEVHDI
                     GVDVMLVIGGGNYFRGAEGERNGIDRATGDHIGMLATVMNGLVLSNALETIGVKTRVQ
                     SAIEIRALCEPYIRRRALRHLEKKRVVIFVAGTGNPFFTTDTAAALRAIEMNANAILK
                     ATKVDGVYDKDPKRYKDAKKYSAVSYLDVIRQQLRVMDTTATSLCMENKLPIVVFNMT
                     KAGNIRKVVTGEKIGTVVS"
     gene            1410..1982
                     /gene="frr"
                     /locus_tag="PK590_04970"
     CDS             1410..1982
                     /gene="frr"
                     /locus_tag="PK590_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020309739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOG23983.1"
                     /translation="MADFAQVKTLLEDMKKKMAKGVEATKKEFQNIRTGRASIALVEG
                     ISVNYYDTPTPLKNIGTISTPDARTIQITPWDATALPEIEKAILKSDLGLMPMNDGKV
                     IRIQIPPLTDERRNELTRVVRKVAEEGRVSIRTSRHEAIEQVKKLEKAKTIPEDVSFA
                     GQKEIQKVTDQVVAQIDEALKQKEAELGAI"
     gene            2179..2252
                     /locus_tag="PK590_04975"
     tRNA            2179..2252
                     /locus_tag="PK590_04975"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2213..2215,aa:Lys,seq:ttt)
     gene            complement(2501..4090)
                     /locus_tag="PK590_04980"
     CDS             complement(2501..4090)
                     /locus_tag="PK590_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOG23984.1"
                     /translation="MEENKEVKPLRCAIYTRKSTTEGLDSDFTTLDNQRESAESYIKS
                     QHNQGWVLLKDLYNDGGFTGANTDRPALQKLLQDIREKKIDCVVVYKVDRLSRSLSDF
                     VKLLQFFEEQQVTFVSITQHFNTNTSMGRLTLNILLSFAQFEREMISERVKDKMGAAR
                     KKGRWVGGLPGLGYEIDKPTKKLVINPKEATLVRRIFQLYLEGNSLLKIAQKLNAEGL
                     RTKVYPGRKNNSGGVLFRNTNIQLILRNPTYMGKIQYEGKLYQGLHEPIVSEEIFKKV
                     QEALKANFRARGISTNRKFPGLLTGLLRCACCNTSLGHTYARGRNKNFNYRYYVCLYS
                     IKQNRAECPSKSLIASVTEDKFIGFLIRLTKDKRLMDESWATLPLEKQSVILKELVKG
                     IEYDGNKGKIYIELAGTFEKHEFDLPLDELKKRSPDFTKIAFNHEPLIRKQLLLAHQI
                     QGMLADGRAKDLKQIAGWTGISLFRLYQILNLLYLCPKIQEDILTAPNSRLSEITERN
                     IRHISNEIDWQKQAALWNQPH"
     gene            complement(4077..4544)
                     /locus_tag="PK590_04985"
     CDS             complement(4077..4544)
                     /locus_tag="PK590_04985"
                     /inference="COORDINATES: protein motif:HMM:NF022598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2924 domain-containing protein"
                     /protein_id="HOG23985.1"
                     /translation="MQANTAERIEELKTAGAEQLPLLYRRYFGDKPAPGNRTFLIRQL
                     AYRIQEKASGELSPTAKCKIQELIHTYDPINRTVIRSSTGTTETGRDIRLPTPGSFIT
                     KVYKGKRLDVKVLEKGFEYQDTVYSNLSAVAKAVTGAHWNGFVFFGVKNRGRK"
     gene            4632..5201
                     /locus_tag="PK590_04990"
     CDS             4632..5201
                     /locus_tag="PK590_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOG23986.1"
                     /translation="MKRQNTRYFTGKEKATFLRFLEKMRDGRRAEMLYKLMFATGLRL
                     AETVSLNVGDVQEKDLIAILGKGDKLREIPLSKAIQTKIEQFLAWKNRKGEDISIKAP
                     LFVSRKGGRLSRRAVQWDLNLWVKRAGLEGKYSPHALRHTVGFELMRKTRNIRQVQEF
                     LGHSSITTTQIYTHVTKEELRHCAELLDI"
     gene            5476..>5699
                     /locus_tag="PK590_04995"
     CDS             5476..>5699
                     /locus_tag="PK590_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23987.1"
                     /translation="MQKYQDLVIQAKREDRTISQFIKLRLKRNLDKVHSIRPADVTFR
                     NSKQIPFQRWYPYTEGYSPDFVTTLLEKYCS"
BASE COUNT         1444 a         1285 c         1535 g         1435 t
ORIGIN      
        1 aaaaggccgc gctttccgag gcgaaagggg atctcaagac cgcgcaggat attcttcgaa
       61 aaaaggggct tgatatcgcg aaaaagaaat ccacgcgtga ggccagggaa ggccagattg
      121 tttcctatat tcattcggga gggaaactgg gcgtgattct cgaagtgaat tgcgagacgg
      181 attttgtcgc gaaaaacgag actttccagt ccttttgccg tgatctcgcg atgcagatcg
      241 ccgcggccca tccgttgttt gtgtcgccgg aaagcattcc ctccgaaatc atggagcggg
      301 aaaaggcgat ttttctggat caggtcaagg ataaaccggc gccggtccag gcgaagatta
      361 ttgaaggaaa actggcgaag cgctatgagg aaatctgcct tctgaaccag aagtttatca
      421 aggacgattc gaagaccgtc caggatattt tgaccgcgat gatcgccaaa atcggcgaga
      481 atattctggt gaggcgattt gtccgctttg aagttggcgg taattaacct gagggaacgg
      541 gtaacggata atggggagtg aaagcaggag ccattccccg aagaatgttt cccgtcaaag
      601 taatgaaaaa ggccgtctat aaacgcattg tactgaaact gtccggagag gcgcttgagg
      661 gttcccggga ctttggcatt gatcccgagg tcgtaaagtc tatcgcccgg cagatcaaag
      721 aagtccacga catcggcgtg gatgtcatgc ttgttatcgg cggcggaaat tatttccggg
      781 gcgccgaagg cgagcggaac ggcattgacc gcgccacggg cgatcatatc gggatgctcg
      841 cgacggtcat gaacggcctt gtgctttcga acgcccttga gacaatcggg gtcaagaccc
      901 gggtgcaatc ggcgattgaa atccgcgcgc tttgtgagcc gtatattcgc cggcgggcct
      961 tgaggcatct ggaaaagaaa cgggtggtta tttttgtggc gggtaccggg aatccttttt
     1021 tcacgaccga cacggcggcg gcgcttcgcg ccatcgaaat gaacgcgaat gccatcctga
     1081 aagcgacaaa agtggacggg gtttacgaca aggatccgaa gcggtacaag gacgcgaaaa
     1141 aatattcggc cgtcagttat ctcgacgtta tcaggcaaca gctccgggtg atggatacga
     1201 cggcgacctc gctttgcatg gaaaacaagc tcccgatcgt tgtttttaat atgacgaaag
     1261 caggtaatat ccgcaaggtc gtgacggggg agaagatcgg aacggtcgtg agttgattgg
     1321 gtggacctgg gtcggtcgaa ccctcgtgga ccggtttgcg gcgaaaaccg gccgaatgtc
     1381 gtgaaaatat gtattcaagg aggtaattta tggcggattt tgcgcaagtg aaaactctgc
     1441 tcgaggacat gaagaagaaa atggccaaag gcgtcgaggc gaccaaaaaa gaattccaga
     1501 atatccggac gggacgcgct tcaatcgctt tggtggaggg gattagcgtc aattactacg
     1561 atacgcctac gccgctcaag aacatcggga ctatcagcac gcctgacgcc aggacgatcc
     1621 agatcactcc gtgggacgcg acggcgcttc cggaaatcga gaaggcaatc ctcaagtcag
     1681 atcttggtct gatgccgatg aatgacggta aagtcatacg gatccagatc ccgccgctaa
     1741 cagatgaacg gcgaaacgag ttgacccgcg tggtcagaaa agttgccgaa gaagggcgcg
     1801 tgtcgatccg aacttcccgc catgaggcga ttgaacaggt taagaagctt gaaaaggcca
     1861 agacgatccc tgaagacgtt tcgtttgccg gccagaaaga aatccagaaa gtgacggacc
     1921 aggtcgtcgc gcaaattgat gaggctttga agcagaaaga agcggaactg ggcgcgatat
     1981 agttcgttat tcgtcgcttg aaaagctgga tttttatttt gtcatacgtg ggccccccga
     2041 ccagattttt gtccaaggga caaaaatcaa gaatcggacg ggctccgcaa tcctcgatca
     2101 ggaaattaat gattgcggag gtagcttctc gcgtgcaagc tggtttttta ttgttttaga
     2161 tggcgtcaaa aaattcgtgg gcccgtagct cagcctggta gagcacgtcc cttttaagga
     2221 cggtgtcgat ggttcaaatc catccgggct cacttctctt gtcactctct accggacaga
     2281 gataaaatcc cccaaggaca ccgggggatt tttagtttat agcccatctg atccccatca
     2341 tttccagccc aaatctgccc tctatctcta aaatgccgat tggtaaatgc ccgcaggtta
     2401 atggttcgcg cttcgatatg cgggaaaggg gaagtgccaa aacagaccat taaccagaaa
     2461 tcggagaata attctcggat catgggattt atatctctgg ttaatggggc tgattccata
     2521 gggcggcttg tttctgccaa tcgatctcat tcgaaatatg gcggatattc cgctcggtta
     2581 tctcggacaa acgggaattg ggggcggtaa ggatgtcttc ctgtatcttg gggcagagat
     2641 aaagcaggtt caggatctgg tacaggcgga aaaggctgat gccggtccaa ccagcgatct
     2701 gtttgaggtc cttggctcga ccgtcagcca acatgccttg gatttggtgg gcgaggagga
     2761 gctgtttgcg gatcagaggc tcatgattga aagcgatttt agtgaagtca ggagaccttt
     2821 tctttagctc atctaaaggt aggtcaaatt cgtgtttttc aaacgtgccc gccaattcaa
     2881 tgtaaatctt tcccttattc ccgtcatact cgatcccttt gacgagctct ttcaaaatca
     2941 cggactgctt ttcgagcggg agtgttgccc aggattcatc catcaggcgc ttgtccttgg
     3001 tcagtctgat caaaaagcct ataaatttat cttctgttac ggatgcaata agcgatttac
     3061 tgggacactc cgcacggttt tgtttaatgg aatacaggca aacgtagtag cggtaattaa
     3121 aatttttgtt tcgaccgcgg gcgtaagtgt ggcccaggct ggtgttgcaa caggcgcatc
     3181 gaaggagacc tgttaagagt cccgggaact ttcggtttgt agaaataccc cgtgcccgga
     3241 agtttgcttt tagggcttcc tgcacctttt tgaagatttc ttcgctgacg atgggttcat
     3301 ggagcccctg gtaaagttta ccctcatatt ggattttgcc catataagtc ggattgcgga
     3361 ggatgagctg gatattcgta ttcctgaaaa ggacaccccc cgaattgttc tttcggccag
     3421 ggtatacctt ggtgcggagg ccttcggcgt tcaacttttg tgcgattttt aataatgagt
     3481 taccttcaag atagagttgg aagatccgcc tgacgagtgt tgcctcttta ggattgataa
     3541 ccagtttctt ggttggctta tcgatctcat agccgagtcc gggcagcccg ccgacccatc
     3601 tgcctttctt ccgagccgcg cccattttgt ccttaacacg ttcactgatc atttcgcgtt
     3661 caaattgggc gaaggaaagt aggatattca gcgtgaggcg tcccatggaa gtgttcgtgt
     3721 tgaaatgttg agttatggag acaaaggtaa cctgctgttc ttcgaaaaat tgaagcagtt
     3781 ttacgaaatc ggagagagag cgcgacagcc gatcaacttt atagacgacg acacagtcga
     3841 tcttcttttc ccgtatgtct tgcaggagtt tttgaagggc ggggcgatcc gtattcgcac
     3901 ctgtgaaacc gccgtcgtta taaagatctt tcagtaaaac ccagccttga ttgtgctggc
     3961 tctttatgta gctttccgcg gattcgcgct ggttatcgag agtagtaaag tcgctatcga
     4021 gaccttctgt tgtggattta cgggtgtata tcgcgcagcg aaggggctta acttctttat
     4081 tttcttccac ggttctttac tccaaaaaag acgaacccgt tccaatgggc tccggttact
     4141 gctttggcca cggcgcttag gttgctgtaa accgtgtcct gatattcaaa tcccttttcc
     4201 agaactttca catcaagccg ctttcccttg taaactttgg taataaatga ccccggtgtg
     4261 gggaggcgaa tatcccggcc cgtttccgtg gttccggtag aggaccggat aacggtcctg
     4321 ttaatcgggt cataggtgtg gatcaattcc tggatcttac atttggctgt aggggagagt
     4381 tctccggacg ctttctcctg gatccggtaa gccagctggc ggatcaagaa agtccggttc
     4441 ccgggtgcgg gcttatcccc gaaatatcgc cggtaaagca gcggtagctg ctcggcgcca
     4501 gccgttttta gttcctcgat tcgctctgct gtattagctt gcataattgc ctcctctacg
     4561 catgtatatg tccatacctc ttgggatata gcaagtttat aaggttggta ttagataggc
     4621 ggagataggt tatgaaacgg cagaatacgc ggtactttac gggcaaagaa aaggcaacct
     4681 tcctgaggtt tcttgaaaag atgcgagatg ggaggcgggc cgagatgctt tacaagctta
     4741 tgtttgcgac cggtcttagg ctcgcggaga ctgtaagtct caatgtcggc gatgttcagg
     4801 aaaaggatct tatcgccata cttggaaagg gggataagct gcgagagatt ccgttatcca
     4861 aagccatcca aacgaaaatc gaacagtttc tggcctggaa gaatcgaaaa ggggaagaca
     4921 tctcgatcaa agcgccctta tttgtttcac gcaagggcgg caggcttagc agaagggccg
     4981 ttcagtggga tctcaatctt tgggtaaaac gcgccggtct ggaagggaag tattcgcccc
     5041 atgcccttcg gcatacggtt ggctttgaac ttatgcgtaa aacccgcaac atccgccagg
     5101 tccaagaatt cctcggtcac agctccatca ccaccaccca gatctacacc cacgtcacca
     5161 aagaggagct ccgccactgc gccgagcttc ttgatattta atcaaggcgg tccgactacg
     5221 acatgatgtt gtcgcgacaa gtgatattgt tgttatacaa cagaagggtg ggagtaatga
     5281 aaattcgatg taactatcat actatggtat attgtatcca tgaataggca gaattttaaa
     5341 tccatatatt tcacagacaa agatcttttt ggaacaagcg gccgctcggt gaccgtttct
     5401 ttttcagaga aagattctgt attaaaagat caggtgcaat gcttgtccaa tgaagaaatt
     5461 agggaactaa tcgggatgca gaaatatcaa gatcttgtta ttcaggcaaa gcgggaagat
     5521 cgaaccataa gccaattcat caaattgcgg cttaagagaa atctcgataa agtacactca
     5581 attagacccg ccgacgtgac gtttcgaaat agtaaacaga ttcccttcca gaggtggtat
     5641 ccgtataccg aaggatattc gcctgatttt gtaactactc tattggaaaa atattgctc
//
LOCUS       DAOSIO010000109         5678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_12802_length_5678_cov_4.158812, whole genome shotgun sequence.
ACCESSION   DAOSIO010000109 DAOSIO010000000
VERSION     DAOSIO010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12802_length_5678_cov_4.158812"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PK590_05000"
     CDS             <1..194
                     /locus_tag="PK590_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23988.1"
                     /translation="KIANYAGVYQPEGLDVYMKRYFDLFAAKLDQLSAEINQLQKKVD
                     SLMGIGTGTGGILSSGQKS"
     gene            292..1377
                     /locus_tag="PK590_05005"
     CDS             292..1377
                     /locus_tag="PK590_05005"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspK"
                     /protein_id="HOG23989.1"
                     /translation="MKTIKRWIKPCHQRPGSRRGSEGSVLVVSFFVLALLAMFMMTVG
                     YVARQRFQMVSRLDTRQKLRCIGDSGVQKAIHVLVMHRERHPTYDSLNQSWSRNEPEF
                     KDVKVGDGFFSVYRSEDFPLDAESSGEEPPAQGLVDEESKININAVRLTDILRRLFRE
                     ATGLSDGEVAAWVDSLEDWIDEDNDTRSLGAEERYYRGLNPSYLPRNGALATLSELQW
                     IKGITPEIYRKMKPYITLHSSGRVNLNTASEPVLSALGFIPSLVSKIKNYRSGRDGLI
                     GTEDDQVFDDVSTAAQQLAREGYLSDNERANFDAVVQSGFLSVKSQFFTAQVLARLEH
                     KKQAMRVVATFDERGMIKRWEETFVGS"
     gene            1350..2501
                     /locus_tag="PK590_05010"
     CDS             1350..2501
                     /locus_tag="PK590_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOG23990.1"
                     /translation="MGRNIRWLLITGLLLLGIFPLTALLAQDEGGLIPELKLFSKALS
                     AICEAYPGDVAARNLFYEAVKGMMSSLDDFSEFIDPERYKLLEIHMRGEYAGIGAILQ
                     IVQERVTVRAVEPGKPAEQAGLRAGDVILKVDGISVEKKSVAEVSALLRGTENTPVVL
                     TVLREPAVEPFDLTVQRQKIQIPAIQDARMIGKHLAYFRLTAWQDHTIEQVDQTLKDF
                     KENGMKALIIDLRNNDGGLLPQAVALAERFLTQGKKIVSVQSKIQEQKKEYFVAGDGE
                     YAEIELVVLVNEKSASASEVFSAAMQDHRRAVIVGVKTFGKGSVQSVIPLDDVSGMKL
                     TTARYLTPSGRMIDKIGLTPDRVVVNGPEGVPGMDHQTLEAIAVLKNYM"
     gene            2590..3093
                     /locus_tag="PK590_05015"
     CDS             2590..3093
                     /locus_tag="PK590_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23991.1"
                     /translation="MKKQTGLVVVLIVVAVAIGGTYYRLNVKADKTTLNTTVKSQDAS
                     QGSGIPFPEKMNQLSRNMSLWGRLSPEEKTQAVDAVIALYKNRDNIAILNTGEFYVGK
                     IDETIRGNPPVANMDIMTLMRILSIMEYDFYNGQNKDELARNTLGDKGYEDNRTRRRM
                     TAMLGAR"
     gene            complement(3159..4577)
                     /locus_tag="PK590_05020"
     CDS             complement(3159..4577)
                     /locus_tag="PK590_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23992.1"
                     /translation="MTFFSAFIFPPLLGCLLIAPFLQHTRFPQKILFLSAVSFPIGYG
                     ACSLLLFFAYLVAPPVATWTTLVAGLGIMIFSVKKLLSSFRPTRLSFWAKSIGPFSLP
                     KFNLLPTVFLITGFLLFVFILIRTLDFYSLTISLNVFGGWDAYYFWALKAKFMFRAPA
                     EWQNLFSPDLFWTHPDYPLLLPGSLAWGWLWLGKEAFLWAPFLAIGFYLSCAFLLIWY
                     LTSTVSAFTGYLGGSFFLILGPCFFWAVHFYADVPLSFFIAGATLTAVIALREKEEKL
                     WRVSGLLSGLAAWTKNEGILFILSFSCLLMAILFFEKIKSKRQFSRCLYCFLSGAILP
                     VLAILILKTFLGTTGDYLGSGRTLFDYLTLLFSGWDRVLLLLNAYRKNVTSFTLWRGL
                     WGFFLIAVFYLGFRNGRSRYKWVPVGIFLLVNLGYAVAILTSPYDLEWQIQTAFERLL
                     LHTVPLVLAFSFETLALSPNHQ"
     gene            complement(4574..5038)
                     /locus_tag="PK590_05025"
     CDS             complement(4574..5038)
                     /locus_tag="PK590_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23993.1"
                     /translation="MLKDRLKVERNGLPSWTRIARVVFYGVAFLLVITSRKPSAPPQA
                     YPLRRILFGGHSAMFKSPGFEFGSLKPFLPQTGKVSFIMDIPYDPHDARMEPFCAAQA
                     HLAPLVLTPFPSERIAIINCSNAAMAIRRSHETNYRIIQGPSGGKAIAVKQP"
     gene            complement(5204..>5678)
                     /locus_tag="PK590_05030"
     CDS             complement(5204..>5678)
                     /locus_tag="PK590_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23994.1"
                     /translation="HYLGFAGNEALKEPFLEDFAMMKKTKESGARGLCAFGMEVYGTR
                     MYDSFNSLWRGWRRIYLHAFEQNPWRLSRCVSSVFFFSVLPFLILPFLAGHNAAFIAG
                     ALLVGILLATAFKTHSIVRANRMAALLHPLAAMVFTIILFDSFLMAALKKPTFWR"
BASE COUNT         1548 a         1242 c         1642 g         1246 t
ORIGIN      
        1 agaagatcgc caattacgcg ggtgtttacc agcccgaggg cctggatgtc tacatgaaac
       61 ggtactttga tctgtttgct gccaagctcg accagctgag cgcggaaatt aaccagcttc
      121 aaaaaaaggt ggacagtttg atgggaatag gaacggggac gggagggata ctttcttcag
      181 gacagaagtc ctgagccttt tccaaagtga aaggaagtaa gtgaggacgg gcgggaaacg
      241 tgaaactttc tgaaagcgat gccattgcga catgagagga aagaaggcgg aatgaaaact
      301 atcaaaagat ggattaaacc ttgtcatcaa cggcctggtt cgcgcagagg ctcagagggg
      361 agtgtccttg tcgtatcttt ttttgtgctc gcccttcttg ccatgtttat gatgacggtg
      421 ggctacgtcg cccgccagag atttcagatg gtatcgcggt tggatacccg acaaaagcta
      481 cgctgcatcg gggactcggg tgtacaaaag gcgattcatg ttttggtgat gcatcgtgaa
      541 cgtcatccta cttatgactc gctgaatcaa agctggagcc ggaacgagcc ggagtttaaa
      601 gacgttaagg ttggtgatgg tttcttttcc gtttatcggt cggaggattt tcccctcgac
      661 gctgaaagct caggcgaaga acccccggcg cagggtctcg ttgatgagga aagtaagatc
      721 aacatcaacg cggttcgctt gacggatata ctgaggagac tttttcgcga ggctacgggt
      781 ttgtcggatg gcgaagttgc cgcgtgggtt gattcgctag aggactggat tgatgaggat
      841 aatgataccc gttcattggg cgccgaggaa cgttactatc ggggtttaaa tccatcctat
      901 cttccccgaa acggggcgtt ggcgacactc tcggaattgc aatggatcaa aggaattacc
      961 cccgagattt atcggaaaat gaaaccatat attacactcc atagttccgg acgggtcaat
     1021 ttgaatacgg cttcggagcc ggtgctttcc gcgctgggtt ttattccttc gctggtcagt
     1081 aaaattaaaa attatcgttc gggaagagat gggttgatcg gcacggaaga tgaccaggtg
     1141 tttgacgatg tatcgaccgc ggcgcaacag cttgccaggg aggggtattt gagtgataat
     1201 gaacgcgcga attttgatgc cgttgtccag tcgggatttc tttcagttaa atcccagttt
     1261 tttaccgccc aggttcttgc caggttggag cataaaaaac aagcgatgcg ggttgtggcg
     1321 acttttgacg aaaggggaat gattaaacgg tgggaagaaa cattcgttgg ctcctgatta
     1381 ccggattatt gttgctcggc atctttccgc tcacggcgct cttggcccag gatgagggtg
     1441 gattgattcc tgagctcaaa ctgttttcaa aagccctgag cgccatttgt gaagcctatc
     1501 ccggggacgt agcggcccgt aatttgttct acgaagcggt taaggggatg atgagctctc
     1561 tggacgattt ttcagagttt attgacccgg agcgctacaa acttcttgaa attcacatga
     1621 ggggtgagta cgcggggatc ggcgccattc tccagatcgt tcaggagcgg gtgacggttc
     1681 gtgccgtgga acccggaaaa cccgcggagc aggccggcct tcgggcggga gacgtgatcc
     1741 tgaaagtgga cgggatttcg gtcgagaaaa aatcagtcgc cgaagtttca gcccttttgc
     1801 gtggcaccga gaatacgccg gtggtgctta ccgtgcttcg cgagcccgcg gttgaacctt
     1861 ttgacctgac ggttcagcgc caaaaaattc agattccggc gattcaggac gcgagaatga
     1921 tcgggaagca cctggcgtac ttccgtctga cggcttggca ggaccacacg attgagcagg
     1981 tcgaccaaac ccttaaagat ttcaaagaaa acgggatgaa agcgctgatt attgatcttc
     2041 ggaataatga cggagggctt cttccccagg ctgtggcgtt ggcggagcgt tttttaacgc
     2101 agggaaaaaa gatcgtatcg gttcagagca agatccagga acagaaaaaa gaatattttg
     2161 ttgccgggga tggtgagtac gcggagatcg agttggtggt attggtgaat gaaaaaagcg
     2221 cgagcgcctc cgaggttttt tccgcggcga tgcaggacca tcgacgcgcc gtgatcgttg
     2281 gcgtgaaaac gttcggaaag ggatcggttc agtccgtgat tccacttgat gatgtcagcg
     2341 gtatgaaatt gaccacggct cgttatttga ccccttcagg acggatgatc gacaagattg
     2401 gattgacccc ggatcgcgtg gtcgtcaacg gcccggaggg ggttccgggg atggatcacc
     2461 aaaccctgga agcgatcgcg gttttgaaaa attatatgtg acgctacggg agcggtcgtg
     2521 tctttttaac gtcagtattc tggtttcatc tcgggatcga tatttcttat cacttaggag
     2581 gaaaattgta tgaagaaaca gacgggtttg gttgttgtat taatcgttgt cgcggttgcc
     2641 atcgggggaa cctattaccg cttgaacgtg aaggctgata aaacgacgct gaataccacg
     2701 gtaaagtcgc aagacgccag tcagggatcc ggtatccctt ttccggaaaa aatgaatcaa
     2761 ctgagccgga acatgtcatt atgggggaga ttgagcccgg aggaaaagac gcaagcggtc
     2821 gacgcggtga tcgccctcta caagaaccga gataatatcg ccattctgaa taccggtgag
     2881 ttttatgtcg gaaaaattga tgaaacgatc cgggggaacc ctcccgttgc caatatggac
     2941 attatgacgc tgatgcgtat tctttcgatc atggaatatg atttttacaa cgggcaaaat
     3001 aaagacgaac ttgcccgcaa cactcttggt gacaaggggt atgaggacaa ccgaacgcgc
     3061 cggagaatga cggcgatgtt gggcgctcgt tagcggcgtt tgggttgttc gagaatcagg
     3121 aaaggcaggt gcctgtcatg gacggacgcc tgcctttttt attgatggtt tggggagagg
     3181 gctaaggttt cgaaactgaa ggctaaaacg agagggactg tgtgtaacag aagacgttca
     3241 aaggctgtct gaatttgcca ttcaaggtcg taaggggacg taagaatggc taccgcgtat
     3301 ccgaggttaa ccagcaaaaa aattcccacg ggcacccatt tatagcgaga gcggccgttt
     3361 ctgaaaccca aataaaaaac agcaatcagg aaaaatcccc acaagccgcg ccagagggta
     3421 aatgaagtga cgtttttccg gtaggcattg agcaaaagaa ggacacggtc ccatcctgaa
     3481 aaaagaaggg ttaaatagtc gaagagagtt cgtcccgatc cgaggtagtc tccagtagtg
     3541 cccagaaatg ttttcaatat caaaatggcc agaacaggaa gaatcgctcc ggacaggaaa
     3601 cagtaaaggc accgcgagaa ttgcctcttc gactttattt tttcaaaaaa cagaatagcc
     3661 attaaaagac aactgaacga caggatgaac aggatgccct cattttttgt ccaggccgcg
     3721 agtcccgaca aaagtcctga aaccctccat aacttttctt ccttttcacg aagggcgatg
     3781 acagcggtca aagtcgcgcc cgcgatgaag aatgataaag ggacgtccgc gtagaagtgc
     3841 accgcccaaa aaaaacaggg cccgagaata agaaaaaagg atccccccag ataccccgtg
     3901 aacgcggaca ctgtcgaggt caaataccag atcagaagga aagcgcagga caggtaaaat
     3961 ccaatggcga gaaagggggc ccagagaaat gcttctttac ctaaccagag ccaaccccag
     4021 gctagggaac cgggtaaaag aaggggataa tcagggtgag tccagaaaag atcaggggaa
     4081 aaaagatttt gccattccgc gggcgcgcgg aacatgaatt tagctttgag cgcccaaaaa
     4141 tagtaggcgt cccatccgcc gaaaacattg agcgagattg tcagagagta gaaatcgagt
     4201 gtcctgatga ggataaaaac aaaaagcaaa aaacccgtta tcagaaagac agttgggagg
     4261 aggttgaatt ttgggagtga aaatggcccg atggattttg cccagaacga aagacgggtt
     4321 ggcctgaagc tggacagaag ttttttgact gaaaaaatca tgatgcctaa ccccgcgaca
     4381 agcgtggtcc acgtcgcgac cgggggagcg acgagataag cgaagaaaag cagaagagaa
     4441 catgcgccat agcctatcgg gaaagagaca gcgctcagga ataaaatttt ttggggaaac
     4501 cgggtatgtt gcaaaaaagg cgcgatcagc agacaaccaa gcaaaggagg aaaaataaag
     4561 gcggaaaaaa aagtcatggt tgtttgacgg cgatagcctt gccgccggag gggccctgga
     4621 tgatccggta attggtttca tggcttcggc gtatggccat cgcggcgttt gaacaattaa
     4681 tgatcgcgat gcgctcggat ggaaaggggg ttagcacaag gggcgccagg tgagcttgcg
     4741 cggcacagaa aggctccatg cgggcgtcgt gtggatcgta gggaatatcc atgataaaac
     4801 tgacctttcc cgtttggggc agaaaaggtt tcaatgaacc gaattcaaaa ccgggagatt
     4861 tgaacatggc cgaatgtcca ccgaacagta ttcggcggag cgggtaagct tgcggaggcg
     4921 ccgatggctt tcgggaggtt atcaccagaa gaaaagcgac cccgtagaat acaactcgcg
     4981 cgatccgcgt ccaggacggc aggccgttcc gctccacttt taatctgtct ttaagcataa
     5041 aactgttccc gggtgtagcg agccaagtct gaaaaatcag cgaaagaaac gccacaaatt
     5101 cctgttgaag agtagcaccg ccgaattata acggtttgat tttgtcgcac aagattattc
     5161 tcgggtttcc cgggccggga acgcgggcgg ggagcgttac ccgctatctc caaaaggttg
     5221 gttttttcag cgcggccatg agaaacgaat caaacaggat gatcgtgaat accatagcgg
     5281 cgagcggatg caggagcgcc gccatccgat tcgctcgaac gatggagtgg gttttaaaag
     5341 ccgtggctaa caggatcccg acaagcaggg cgcccgcgat gaacgcggcg ttatgacccg
     5401 ccagaaaggg caggatgaga aagggaagaa cggaaaagaa aaagacgctt gaaacgcatc
     5461 gcgagagacg ccaggggttt tgctcgaagg catgcagata aattctcctc cagccgcgcc
     5521 agagactgtt gaatgagtcg tacatccgcg tcccgtaaac ttccattcca aacgcgcaga
     5581 ggcccctcgc gcctgattct ttcgtttttt tcatcatcgc gaaatcctcg agaaagggtt
     5641 ctttgagagc ctcattcccg gcaaaaccga ggtagtgg
//
LOCUS       DAOSIO010000110         5644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_12889_length_5644_cov_3.477187, whole genome shotgun sequence.
ACCESSION   DAOSIO010000110 DAOSIO010000000
VERSION     DAOSIO010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12889_length_5644_cov_3.477187"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..1221
                     /locus_tag="PK590_05035"
     CDS             178..1221
                     /locus_tag="PK590_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23995.1"
                     /translation="MKSERWVIVFSLAIFFVGICGVKAETISSDYPDFKTVANYENMK
                     TVEAKTGLFDMPFGISLAGVLKLAEARGMSILGPTSGSLSEEEKEKLRAGVDIKKESS
                     LRDDTEFYFGNNWYKLESVLGSIGKCEFAAGEKKTYKILFSPSAEMKKMGVSIFIVFL
                     FGDMPAEELKVYAEAALFQPDLFGKADGVGLILMDALQSKYGRPLVILNTKNFYLPLP
                     ISDDDLHGKKYLRDQYHKIWFSVNGPGSPLLPGGAEILLDEKVYTAMDESLFRLTQFE
                     RRGWTFACWARNITMVVVAHDRQEYYGLFYFDSDPNIAKTILSSQYSALARCAKDEKE
                     EYAKTKQKAQGSL"
     gene            1284..2318
                     /locus_tag="PK590_05040"
     CDS             1284..2318
                     /locus_tag="PK590_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG23996.1"
                     /translation="MRTKFAWAFLLTLVLIAWAHKQTIASEHEDWLIADFDSQAKTNN
                     AGGGFFTSSKRAVDSISMSFAEGDALNNPAGHSMRINYSIADTDDPFAFFAVDSAPTT
                     MVLSDEKPKFINFFIKAPKGSPYHFRAVVFGKNEFGRLTFVERKNIGGTGSDTWTVFS
                     VPLKRGPYATSGEAFSCKIELQILFDYIGIRKNALAGTFFIDQITLSDRNLEKEAAEV
                     RKQADEARKAEKLEKHRKADQEKKKTKQIASLTEEAENAQRDAEKAQRKAEKAQRKAD
                     GTQQDAEMSRKQAAKWFVSEEVKRKAEIFSKKAEEAKLKAEEAQQEAEKAKQYADMMA
                     KRVAAFKAEQ"
     gene            3046..4638
                     /gene="istA"
                     /locus_tag="PK590_05045"
     CDS             3046..4638
                     /gene="istA"
                     /locus_tag="PK590_05045"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOG23997.1"
                     /translation="MANELKMAIVNTITGLLEKGWKHRRIARELGVNRETVGRYARLR
                     REIGSKPANPTPGSEGPPAPNPANPCLSGGQATAGFCAGPASLCEPFREIITQKLAGN
                     LEGVRIWQDLVQDGFNGSYSSVKRFLRHLRPTQDLPFRRMECPPGEEAQVDFGTGAWT
                     LVDGKKRRPYIFRIVLSYSRKAYSEAVWRQTTENFIRCLENAFRAFGGAPKTLVIDNL
                     RAAVTHADWFDPELNPKIMDFAKHYGIVILPTKPYTPRHKGKVENSVKYIKNNALKGR
                     SFASLEEQNVFLAAWESQIADRRIHGTTRQQVLKLFGIEKTSLQALPLEAFPFFSEGR
                     RSVHRDGHVEVAKAYYSVPPEYLGHELWARWDSKLVRIFDKDLKQVTVHCRVLPGRFH
                     TLREHLDHRKISGVERGAEYLLSRAFRIGPSTGRWAKAMFDARGIEGIRVMQGLLSMA
                     AKQPVAVLERATEYALNSECFRLRTLRELCQSFSQKHAPESFIQEDPLIRPLSEYQDL
                     LHVSFKPQPPTTKELFNASATH"
     gene            4619..5389
                     /gene="istB"
                     /locus_tag="PK590_05050"
     CDS             4619..5389
                     /gene="istB"
                     /locus_tag="PK590_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HOG23998.1"
                     /translation="MRAQLIENLKRLHLSGIRNTLDVRIQEAAGNQLSPLEFLELVLQ
                     DEITMRNDRAFAKRIKAAQFRDLKTIEDFDFTFNASIKRKQIMDLAAGHFIRQHQDVL
                     LIGPPGVGKSHLAQGLGYQASKMGFTVFYRSIFTAVSELMQAEAMNETAAKLKRYLAP
                     EVLIIDDMGLKQLPKNAGEHLFEIIMRRSELHSTILTSNRPLSDWGKLIGDVPTAGAI
                     LDRLLSRAQVIEISGRSYRLREKTCGKQKDDDNQQSQH"
BASE COUNT         1597 a         1292 c         1425 g         1330 t
ORIGIN      
        1 ccaatttcga aaaagctctg agtgaaatca atcagagctt ttcatattgg taaaacatga
       61 aaagttcgag gaattttgat gcctggaggg cgatgtctga agacccaaaa agggaggaga
      121 cgtaaagcgg aatgcctagg cgtaacagaa ttataatatc tatgggagaa taagctcatg
      181 aaatcagaaa gatgggttat tgttttttcg ttggcaatat tttttgttgg aatttgtggc
      241 gtgaaggccg aaaccatctc tagtgattat ccggatttca aaaccgttgc aaattatgaa
      301 aatatgaaaa ccgtcgaagc gaagacgggt ttatttgaca tgccttttgg gatttcactg
      361 gccggagtgt taaaattagc agaagcaaga ggtatgagca ttctcgggcc aacgtcgggc
      421 agtctttcag aagaagaaaa agaaaaacta cgcgctggcg ttgacataaa aaaggaatcc
      481 tcattaagag acgatacgga attttatttt gggaacaact ggtataagct tgagtctgtg
      541 ctcggcagta ttggcaagtg tgaatttgcc gctggggaga agaaaacata caagatttta
      601 ttttcgccgt cggccgaaat gaaaaaaatg ggggttagca tatttatagt tttccttttt
      661 ggggatatgc ccgcagaaga attaaaagta tatgccgagg cggctttgtt tcaacctgat
      721 ctctttggaa aagcggatgg ggtggggcta attctaatgg atgcgcttca aagtaaatat
      781 ggtcgaccat tggttatttt aaacacaaag aatttttacc taccgctacc gatatctgat
      841 gacgatttgc atgggaagaa gtatttgcgt gaccagtacc ataaaatatg gttctctgtt
      901 aatggcccag ggtcaccgct tttgccgggc ggggcggaaa ttcttctaga tgagaaagtg
      961 tacaccgcaa tggatgaatc gctgtttcga ttgacgcaat ttgaacgtcg cggatggact
     1021 tttgcgtgct gggctcgcaa cattactatg gttgtcgtgg cgcatgacag gcaggaatac
     1081 tatggccttt tttattttga tagtgatccg aatatcgcca agacaattct ttcaagtcaa
     1141 tacagcgctt tagcaagatg tgcgaaggat gagaaagagg aatatgcaaa gacgaaacaa
     1201 aaagcgcagg gcagcttgta acgaagcgag ctggctcagc gccggttgtt gcgtcgcaag
     1261 ggctaaaaga aagggagaac cgcatgagaa caaagttcgc atgggcattt ttactaacgc
     1321 tagttttgat agcatgggca cataaacaaa ctatagcatc ggagcatgag gattggctaa
     1381 ttgctgattt tgattctcag gctaaaacca ataacgcggg cggtggattt ttcacgtcga
     1441 gtaagcgagc cgttgatagc attagcatgt catttgctga aggagatgcc ttaaataatc
     1501 cagccgggca tagcatgcgg attaactata gcatcgctga cactgatgac ccttttgctt
     1561 tttttgcggt tgattctgcg ccaacgacaa tggtgcttag tgatgaaaag ccgaaattta
     1621 ttaacttttt tattaaagcg ccgaaaggaa gtccgtatca ttttagggca gtcgtttttg
     1681 gtaaaaatga atttggaaga ctcacctttg ttgaacgtaa aaatattgga ggaaccggaa
     1741 gcgatacatg gactgtgttt tccgttcccc tcaagcgcgg tccatacgct acttctggtg
     1801 aagcgttttc ttgtaagatc gagctgcaaa ttttgtttga ctatataggc attagaaaaa
     1861 atgcactagc cggaacattc tttattgatc aaataacatt atcagatcga aatttggaaa
     1921 aagaagctgc tgaggttcga aaacaagctg atgaagcgcg aaaggctgaa aaactggaaa
     1981 aacaccgaaa agccgaccaa gagaaaaaga aaacgaagca gatcgcaagt ttaacagaag
     2041 aagctgaaaa tgcacagcga gacgctgaaa aggctcaacg aaaagctgaa aaggctcaac
     2101 gaaaagctga tggaacccaa caagatgctg aaatgtcaag gaagcaggcg gcgaaatggt
     2161 ttgtgagcga agaagtgaaa cggaaggccg aaattttttc gaaaaaagct gaagaagcca
     2221 agctgaaagc tgaagaagcg caacaagaag ccgaaaaagc aaaacaatat gctgatatga
     2281 tggcgaagcg agtggcagcg tttaaggccg aacaataaat gaaatagatt tgttgaaaat
     2341 agtttaagtc gtatggttaa gcatttaact cttcgttttt tacaccgttg ctaaacgtga
     2401 tatcttaaca ccctgaaatt ttctacaaaa ttcctacggt ctctaggtat aaaaattttc
     2461 tgtaatagtt tcgtttcgag cgctacgagg cttcaaaacg ctaccctccc attcaaaatc
     2521 gccttgccgt gaggcttttg aatcaagacc gataccgtag tcagatggtt tgagccgtgc
     2581 ccgggtgccc atgaaaagcg caaattacct cttccaactc accttacctt gagaacactc
     2641 tttgaaaaat cagaacaccc aatcctaaac ttgggaagta ataaggcgct aaagccaaag
     2701 aagacgagtg aaaagaacac cctgatccgt tgaaatggcc ttacctctcc ggatacttac
     2761 cagacaataa aaaagtcagt cagacgttca ggtatttttt tgttcttaat ctacaaattg
     2821 cacttaatgc ccacgctctt agcgtgacgg cttcggttct cacctaggcg tatgatttta
     2881 tgctattctt taagacaagg aggtgtcgaa ttggatcgcc ctgtcaaaca aaccagaaaa
     2941 gagctggcaa ggggcgggca aaaccagcca gtcagggtcg ggtgaaaacc ggccattttg
     3001 aagggagatg ctgtatatca gttctcaaaa ggagaacgat gatatatggc aaacgaactc
     3061 aagatggcta tcgtcaatac aatcacagga ttgctggaga aaggctggaa gcatcgacgg
     3121 atcgcccgcg agcttggcgt caatcgggaa accgttggcc gctatgcccg gctccggcgt
     3181 gaaatcggtt caaaaccagc caacccgacc cccggctcgg aaggtcctcc agctccaaac
     3241 ccggccaacc cctgcctgtc cggcgggcag gcgaccgccg gcttttgcgc gggtcccgcc
     3301 agtctttgcg agcctttccg cgaaatcatc acccaaaaac tcgctggaaa tctcgaaggt
     3361 gtccggatct ggcaagatct ggttcaagac ggatttaacg ggtcttactc ttcggtcaaa
     3421 cgcttccttc gccacttgcg ccccacgcaa gatcttccgt tccggaggat ggaatgccct
     3481 cccggagagg aagcccaggt tgatttcgga accggtgcct ggactctcgt agacggcaag
     3541 aaacgtcgtc cctacatttt ccggatcgtt ctgagttatt cccggaaagc ctacagtgaa
     3601 gccgtctggc gacagacgac ggaaaacttc atccggtgcc tcgaaaacgc attccgggct
     3661 tttggaggcg ctcctaaaac gctcgtgatc gataatttga gagccgcggt cactcacgcg
     3721 gattggttcg atccggaact caatcccaag atcatggact tcgcaaagca ttacgggatc
     3781 gtgatccttc ccaccaagcc ttacaccccg cggcataaag gcaaggtcga gaatagcgtc
     3841 aaatatatca agaacaacgc gcttaaaggc cgctccttcg caagccttga agagcaaaac
     3901 gtttttcttg ccgcttggga atcccagatc gcggacaggc gcattcacgg caccacgcgc
     3961 cagcaagtcc tgaagctttt cgggatcgag aaaacttcat tgcaagcgtt gccgctggaa
     4021 gctttcccgt tttttagcga agggcggcgc agtgttcacc gggacgggca tgtcgaagtg
     4081 gccaaggcct attactcggt gcctcctgag tatctcggcc atgagctttg ggcgcgttgg
     4141 gattcgaagc tggttcgaat tttcgacaag gatctcaagc aggtgacggt gcattgtcgg
     4201 gtgcttcccg gaagattcca cactctgcgc gagcacctgg atcaccggaa aatctccgga
     4261 gtcgagcgcg gcgcggaata tctcctctcc agagccttca ggatcggacc ctcgacagga
     4321 cgttgggcca aagccatgtt tgatgcccgc ggcatcgaag gcatccgggt gatgcaggga
     4381 ctcctctcga tggcggccaa gcagcccgtc gcagtgctgg aacgcgccac ggaatacgct
     4441 ctgaattccg aatgcttccg gctccggacc ttgcgggaac tatgccagag tttttcccaa
     4501 aaacacgctc ccgaaagctt catccaggaa gatccgctga tccgcccgct ttcggaatat
     4561 caggatctgc ttcatgtttc attcaaacca caacccccaa ccacaaagga gctttttaat
     4621 gcgagcgcaa ctcattgaga acctcaaacg gctgcatctt tccgggatcc gcaataccct
     4681 tgatgtccgg attcaggaag cggccgggaa ccagctcagt cctctggagt tcctggagct
     4741 tgtgcttcag gatgagatca ctatgcgaaa cgaccgcgct ttcgccaaac gaatcaaggc
     4801 ggcgcagttc cgcgatctga aaaccatcga ggattttgat ttcacgttca acgcctctat
     4861 caaacgcaaa cagatcatgg atctcgcggc cggtcacttc atacgacagc accaggatgt
     4921 tctgttgatt ggccctccgg gggtcgggaa atcacatctg gcgcaaggcc ttggatatca
     4981 ggcatcgaaa atggggttta cggtctttta tcgctcgatc ttcacggcgg tatccgagct
     5041 catgcaggcg gaggccatga atgaaaccgc ggccaagttg aaacgatatc tcgcgccgga
     5101 ggtcttgatc atcgacgata tgggactgaa acagcttccc aagaacgcgg gagaacatct
     5161 cttcgagatc attatgcggc gctccgaact tcattcgacg atcctgacct cgaaccggcc
     5221 gctctcagat tgggggaagc tgatcggaga tgttcccacg gcaggggcca ttctcgaccg
     5281 gctcctcagc cgcgcgcagg tcatcgaaat ctcaggaaga agctaccggc tcagagaaaa
     5341 aacttgtggg aagcaaaaag atgatgataa tcagcaatca caacattaac cctggccggt
     5401 tttgacccga ccctgactgg ctggttttaa cccgccccgt gacacaattg tcaaactgaa
     5461 ttctgttttc aacgatcata acgctaaaat tggaaatcat gagaccgaag ttaatagggc
     5521 taaggaagaa ctggaaaggc atcttgtcgc cgtcgctata gaagaacaaa actatttgca
     5581 gcttacgacg gaatataact caagcattac agctgctgat gtggctaaaa aagctgtgga
     5641 tgaa
//
LOCUS       DAOSIO010000111         5619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_12953_length_5619_cov_4.554457, whole genome shotgun sequence.
ACCESSION   DAOSIO010000111 DAOSIO010000000
VERSION     DAOSIO010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12953_length_5619_cov_4.554457"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..2812)
                     /locus_tag="PK590_05055"
     CDS             complement(35..2812)
                     /locus_tag="PK590_05055"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOG23999.1"
                     /translation="MNESETRKEIIDLELDKRGWRIANPAEVIVEFEMEKRLDGKPGI
                     AYSGQVYNPKHEYADYLLLGRDGQTPLAVVEAKKTSKNPRVGQRQAEGYADNISKKFK
                     VEPFIFLTNGEATLFWDRLRYPPRKVYGFFERSDMERFNYLRATCEDRPSKIAVNQVI
                     ANREYQLEAIKRIGERLEKKHRKCLLVMATGSGKTRTAMALIDVLLRAKWVSNVLFLA
                     DRKLLRDQAYGKKGFQGFFTEAMDKIKSSSFDKTKRLYAATIQTMEEIYKDISPGFFD
                     LVIMDECHRSIYNKWQDILAYFDAIQIGLTATPSESIDRDTLRFFECEEGRPTFNYSY
                     DEAIEDKVLVPFTPYHAKTSFQIKGLKSGEIPQEIKSKLIAEGKTEEELNFEGTDLEK
                     KFTNKASLESMVQEFMEVCIKDETGVLPGKSIYFAISKEHAYRILDTYDRLYPQYKGK
                     LAEVIVSDDSRSDTFLQRFENESFPRIAISVDMLDTGVDIREAVNLVFAKPVFSKIKF
                     WQMIGRGTRTLDPQNRKEWCQEKDSFLIVDHWSNFEYFNLKPEGEVPQPQDSLPTRIF
                     KLRIQKLKYLTAQKQDCSGIIEEIKISLNALPKDTISIKEKKDVLRRALSDDFWKFID
                     TDFLIREVAPLLRFTEDVNLHVYAFLLKCERLDLALLTQDAEARKEIEGSMREDLRLL
                     PLTLNVVRSKEQEILRASSDAFWKEISRDGVGLLKKDIVPLMKYRKEKREEVIEFDLD
                     DKIIERRWIEFGPHGEGEYVHQYREKVEKHIRDLADKDETLRKIKDGIPVSDEDIEKL
                     EEKLNGPELYITEKVLQDAYEQPAGTFKQFILKLLGKFEFPAREEIVAKAFETFVHGK
                     HYFAADQIRFLRIVKNVFLEKARRREKLTIHDLYEGPFETLGIDAADPLFKKQELEEI
                     ISFLNTQAVLQ"
     gene            3539..4372
                     /locus_tag="PK590_05060"
     CDS             3539..4372
                     /locus_tag="PK590_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24000.1"
                     /translation="MIITERDTEILKMCLEQKFLLLRHIAKKFFPESLSMYKVPARRV
                     NLLMRQGYLKAVKPRVGHDTLYLTTAQGVKHLRSLDLSNGLRPLDGVDLKIYEHDVWV
                     TDARIVLERSPGLIWTPERVLKHKTERRKVPDGLADDGRENSFVIEIERTLKSKRYYD
                     RVLIDTWVKNEGISAFLYLVGKKSMVEWFKKLECCPECFYFATMEDLLRREGNTLFVN
                     KKNSTLILNEHVESDEDFVDEYDGPHKAEAECRRLEKEEFYNQRYKRYKGGAVSDPSG
                     S"
     gene            4619..>5619
                     /locus_tag="PK590_05065"
     CDS             4619..>5619
                     /locus_tag="PK590_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOG24001.1"
                     /translation="MKRAVGYLRRSTDKQEQSLEDQKREVLAFAKREGFEMTGWYADD
                     AISGAFSENRSQFQAMVQEAQEKSRPFDLIIAYDIARFSRGDNIEVGYYLHILRNAGV
                     EVLYVKENLKGDDMDEILRPVFQFNANLYLKSLSRDTIRGQVTCAREARFLGGPVPYG
                     YKRIVNENGKSSLILDSAEKQAVLRRIFDGYVRKEMGYRHIADMLNQERIPAPRGGTW
                     TISCLRSLLMNPIYYGAFVWNRRTLSKFHSVRNGQAEKKPRLRQEKIEHNPESEWFTK
                     ENVYPVIITKDLWLAAQEKRKGRSKFPSQFSGRATTSNYLLSGLITCARCGHTYFGTT
                     "
BASE COUNT         1413 a         1329 c         1425 g         1452 t
ORIGIN      
        1 acgagatgtt taagatagtt ttcttcaaaa gatttcattg gagaaccgcc tgtgtgttaa
       61 gaaagctaat aatttcttca agttcctgtt ttttgaagag cgggtcggca gcatcgatgc
      121 cgagcgtttc aaacgggcct tcataaagat cgtgaatcgt cagtttttcc cgccgccgag
      181 ctttttcgag gaatacgttt ttaacgatcc ggaggaaacg gatctgatcg gcggcaaaat
      241 aatgcttccc atgaacaaag gtctcgaaag ctttagccac aatctcttcg cgggcaggaa
      301 actcgaattt cccgagcaat ttcaggatga attgtttaaa agtgcccgct ggctgctcat
      361 aggcgtcctg caggactttc tccgtgatat aaagctctgg accattaagt ttttcttcga
      421 gtttttcaat gtcttcgtca gaaaccggga tgccatcttt gatctttctg agagtttcgt
      481 ctttatccgc aagatctcgg atatgttttt caaccttttc ccggtactgg tgaacatatt
      541 cgccttctcc atggggcccg aattcaatcc agcgcctttc aatgatcttg tcatccaaat
      601 cgaattcaat gacctcttcg cgcttttctt tccggtattt catcagcgga acgatgtcct
      661 tcttgagcaa accaacaccg tcgcggctaa tttctttcca aaaagcatcg gaggaagcgc
      721 gtagaatttc ctgttctttt gaccggacca cattgagagt cagcggcaat aaccggagat
      781 cttcgcgcat cgacccttcg atttctttcc tggcttctgc gtcttgagtt aacagcgcca
      841 gatccaatcg ctcacatttg agaagaaaag cataaacatg caaattaacg tcttccgtaa
      901 accggagaag aggtgctact tcgcgaataa ggaaatccgt atcgatgaac ttccagaaat
      961 catcagaaag cgcacgcctg agtacgtcct ttttctcttt gatgctaatc gtatccttgg
     1021 gcagcgcatt aaggcttatc ttgatttctt caatgatgcc tgagcaatcc tgtttttgtg
     1081 ccgtcaggta cttgagcttt tgaattcgca acttgaaaat gcgcgttggc aaagagtcct
     1141 gcggttgcgg gacttccccc tcgggtttga ggttgaaata ctcaaaattg ctccagtgat
     1201 cgacaataag aaaagagtct ttctcctgac accattcttt tcggttttga ggatcaagtg
     1261 tccgtgtgcc gcggccgatc atctgccaaa atttgatctt gctaaaaaca ggcttggcaa
     1321 acacaaggtt taccgcctcg cggatatcca ctccagtgtc gagcatatcc actgagattg
     1381 cgattcgggg gaaggattca ttctcaaaac gctggaggaa ggtgtccgag cggctatcat
     1441 ccgaaacaat tacttccgcc agcttgccct tgtattgggg ataaagacga tcataagtat
     1501 ccaaaatgcg ataggcgtgc tctttggaga tggcgaaata gatcgatttt ccgggaagca
     1561 cgccggtttc gtccttgatg caaacttcca tgaactcctg aaccatggat tcaagactcg
     1621 ccttgttggt gaattttttc tcgaggtccg tgccttcaaa gttaagctct tcctcggttt
     1681 tcccctcagc aataagcttg gacttgatct cttgggggat ctcgccggac ttgaggccct
     1741 tgatctgaaa tgaggttttg gcatgatacg gtgtgaaggg gacgaggacc ttgtcttcga
     1801 ttgcttcatc gtaggaatag ttgaaagtag ggcgtccttc ctcgcactcg aaaaagcgca
     1861 gcgtatcgcg atcgatggat tccgagggtg tagctgtcag tccaatctgg attgcgtcaa
     1921 agtaggcgag aatatcctgc cacttgttgt aaatggaacg gtggcattca tccatgatga
     1981 caagatcgaa aaaacccgga gaaatatctt tatagatttc ttccatggtt tgaatggtag
     2041 cggcgtaaag acgctttgtt ttgtcaaagc tggaggattt gatcttgtcc atagcctcgg
     2101 taaagaatcc ctgaaaacct ttcttaccat aggcctgatc acgaagaagt tttcggtccg
     2161 caaggaaaag gacattgcta acccatttag cgcgcaataa gacatcaata agcgccatcg
     2221 ccgtacgcgt cttgccactg ccggtcgcca taacgagcaa acacttccgg tgttttttct
     2281 caagcctctc tccaatgcgc ttgatggctt caagctgata ttctctattg gcaatgactt
     2341 ggttgacggc aattttggaa ggccggtctt cgcaagtagc gcgaaggtaa ttgaagcgtt
     2401 ccatgtccga gcgttcgaag aaaccataca ctttgcgggg cggatatctt agccggtccc
     2461 aaaagagcgt cgcttctccg tttgtcagaa aaatgaaggg ttctactttg aatttttttg
     2521 agatgttgtc ggcatagcct tcagcctggc gctggccaac tcgtggattt ttagaggtct
     2581 tcttcgcttc tacaactgca agaggcgtct gtccgtctct tcccaaaagc agataatcag
     2641 catattcatg cttggggtta taaacctgtc cgctgtaggc aatgccgggc ttgccatcaa
     2701 gacgtttttc catttcgaat tcaacgatca cttcggcggg attggctatg cgccagccgc
     2761 gtttgtcgag ttcgagatca atgatttctt ttcgcgtttc cgattcgttc ataggcaatt
     2821 aggtaacacc ggattaatta ggtattcaat tgggtaattc taaatcaaga actaaatttt
     2881 gtggagaata aaattaaacc gatcatttgg gcgtggtgga atgaggtgag ggagtcgatt
     2941 ccaccacggc aaccacgctt aagtccaatc agcgcttgcc cttcgccctt gttgcatgca
     3001 cccgacccct ccctctctta atctgttgga gcgagatgga ggggtcggct gcattgtggc
     3061 gggcttcggg caagctgttg gagtgtggtt gggtggaata attctttaac ttgttaaggc
     3121 tcattgcttg ttttggactt aatccttcca ggacttttac ccggcaagtc gtggctttga
     3181 gataaaaatt attttgagct taaaaacctt cccaagtcat gaacacccat gtcgtaaccc
     3241 aacctcccat gggggcatgg aaagaggcgg acggagtgaa ggcctcgttg ccaatccctt
     3301 gagaaaaata tttctagctt agaaatgttc gtttagtgtg aacggccatg tcgtaactcg
     3361 acctcccatg agagacctat cagtcaagac cagtccaaaa cctggttgct atcacggcgc
     3421 taaagcccaa gcagtatcgc agaccgagaa aatctgggac tgaaggacat ggattttggt
     3481 ccatatcctg aagttgccgg atgagttcca caattcaagc gaatgaaagc gagcaaacat
     3541 gatcatcacc gagagagata cggagatcct gaagatgtgc cttgagcaga aatttctgct
     3601 tctgagacat atcgccaaga agttcttccc cgagagcctg agtatgtaca aggtcccggc
     3661 aagaagagtg aatctcctga tgcggcaggg ttatttgaaa gcagtaaagc cgagggtcgg
     3721 tcatgacacg ctttatctca cgaccgccca aggcgtaaag cacctgagaa gcctggatct
     3781 gtcgaacgga ctacgaccgc tggatggagt ggacctgaaa atctacgagc acgatgtttg
     3841 ggtcacggat gcgcgtattg tcttggagcg ttctccgggg ctcatttgga cacccgagcg
     3901 cgtgttgaaa cataagacgg aacggagaaa ggtccctgat ggcttggcgg atgatggccg
     3961 ggaaaacagt tttgtgatcg agatcgaacg gacgctgaag agtaaacggt attacgatcg
     4021 ggtgctcata gatacctggg tcaaaaacga gggcatcagt gcttttctct acctcgtggg
     4081 gaagaaatca atggtcgagt ggttcaagaa acttgagtgt tgtcccgaat gtttctattt
     4141 tgcaacgatg gaggatttgc tccggcgcga aggaaacacg ctgtttgtta ataaaaagaa
     4201 cagcacgctc atcttgaacg agcatgtcga gtccgatgag gacttcgtgg acgaatacga
     4261 cgggccacac aaggcggaag ccgaatgccg ccgccttgag aaagaagagt tttataacca
     4321 gcgctataag cgctacaagg gaggtgctgt ttctgatcca tccggctcat aaggatcggg
     4381 atagagctga taaatccaga gatctgacca tcgaagattt tgagatgccc gagccgcctc
     4441 cgggatatgc ccggagcggc tcactcgcta ttcttgcgca atggctggtc cggcattgga
     4501 tagcaaaacg tcaagcgagg aaggcttccg cccacttgac ggcaggtgta ttattcgttt
     4561 ctcgatcaaa gcgccgggct ggcgctttcg atccctgcct gaaaggaggc ctgccatcat
     4621 gaaaagagcg gtcggatacc tcagacgttc cacggacaaa caagaacaat ccctcgaaga
     4681 ccagaaacgc gaggtccttg ctttcgcgaa acgggaaggt ttcgaaatga ccggctggta
     4741 tgccgatgac gcgatctccg gagctttttc cgagaaccgg agtcagttcc aggccatggt
     4801 tcaggaggca caggaaaaat cacgcccctt tgatctgatc atcgcctatg acatcgcccg
     4861 tttcagccgc ggcgacaata tcgaggtcgg ttattacctg catatcttga gaaatgccgg
     4921 ggtcgaagtc ctttatgtga aagaaaatct caagggcgat gacatggacg agatcctgcg
     4981 ccctgtcttt cagttcaacg cgaatcttta tctgaaaagt ctttcaaggg ataccattcg
     5041 cggacaagtc acctgcgcca gagaagcgcg ttttctcggc ggtccggtcc cttacggcta
     5101 caaacggatc gtcaatgaaa acgggaaatc ttcgcttatc cttgatagcg ccgagaaaca
     5161 ggccgttctc aggaggatct tcgacggcta tgtccggaaa gaaatgggct acaggcatat
     5221 cgcggatatg ctgaatcaag agagaatccc tgctcctcgc ggcggaacat ggacgatctc
     5281 ctgccttcgc tctcttttga tgaatccgat ctactacggc gcctttgtct ggaaccggag
     5341 gaccttgagt aaattccatt cggtcaggaa cggccaggct gagaagaagc caaggctacg
     5401 gcaggagaag atcgaacaca accccgagtc ggaatggttc acgaaagaga atgtttatcc
     5461 cgtgatcatc acaaaagact tgtggcttgc ggcgcaagag aagcgcaaag ggcggagcaa
     5521 gttcccgagc cagttcagcg gtcgcgcgac cacttcgaat tatctgcttt cggggctgat
     5581 cacttgcgcc aggtgcggac atacctattt cggaactac
//
LOCUS       DAOSIO010000112         5601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_12997_length_5601_cov_4.765957, whole genome shotgun sequence.
ACCESSION   DAOSIO010000112 DAOSIO010000000
VERSION     DAOSIO010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12997_length_5601_cov_4.765957"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /gene="atpD"
                     /locus_tag="PK590_05070"
     CDS             complement(<1..1117)
                     /gene="atpD"
                     /locus_tag="PK590_05070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOG24002.1"
                     /translation="MSFGKIVQVMGPSVDVEFEAGQLPSILDALEVDFPGTEPRKLVL
                     EVAQHTGDNVVRCIALASTDGLVRGMPVRNTKAPISVPVGNQTLGRVFNLLGDPIDKA
                     GPLENPEKRYVIHRAPPSFEELLPVKSILETGIKVIDLLAPYPKGGKVGLFGGAGVGK
                     TVVVMELIHSIATEHGGVSVFCGVGERTREGNDLWLELKESGVINKTAMVFGQMNEPP
                     GARLRVALSGLSMAEYFRDEEHQDVLVFVDNVFRFTQAGAEVSALLGRMPSAVGYQPN
                     LATEMAELQERIASTKAGSITSVQAIYVPADDLTDPAPATTFSHLDGVTVLSRQIVEL
                     GIYPAVDPLASTSRILDPRIVGEDHYNTAREVQRILQH"
     gene            complement(1197..2060)
                     /gene="atpG"
                     /locus_tag="PK590_05075"
     CDS             complement(1197..2060)
                     /gene="atpG"
                     /locus_tag="PK590_05075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOG24003.1"
                     /translation="MSGQLRTIKTRIRSIESTKKITRAMEMVSAAKLKRFQKLLDQTT
                     PYARALEKLLANILGSSSSCHHPLLTEREEKQCALLVVTSDTGLCGSFNMDLIQETRR
                     FIESRSKKPLLIGFGKAGINALSRDHHTWHTMVKDTKTTEVESAVQEIGLLMGTLFRD
                     QVVDAVYVTRAKFVTQSAHKTVTEKILPFSVQKESEDPVRSLPYIFEPSPEFLFERLI
                     PMIFEVKVREFFLESFIAEQTARMGAMHLATQNAGELIDSLTLTRNKIRQAAITNELI
                     EIVSGSKALKT"
     gene            complement(2118..3620)
                     /gene="atpA"
                     /locus_tag="PK590_05080"
     CDS             complement(2118..3620)
                     /gene="atpA"
                     /locus_tag="PK590_05080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOG24004.1"
                     /translation="MLKPEEVTQIIEKEIQKYQTRLSLESVGYVIQVGDGIARVYGLD
                     EAMAGELVLFHNGVSGMVLNLEPHHVGVVIFGADREIKEGDQVKRTGKIVQVPVGEGL
                     KGRVINPLGDPIDGKGPVISTKMRPIETVPPSVVERQPVKEPLQTGIKAIDSMIPIGR
                     GQRELVIGDRQTGKTAILIDAIINQKDSGVHCVYVAIGQKLSTVVAIRDTLEKYGAME
                     YTTIVSASSEDPAPLQYLAPYSGAAIGEETMRSGKHALVVYDDLSKHATAYRQISLLL
                     QRPPGREAFPGDVFYLHSRLLERAAKLHDDLGGGSMTALPVIETQLGDISAYIPTNVI
                     SITDGQIYLEGDLFFAGVRPAINVGLSVSRVGGNAQTKAMKQVAGMLRLALAQYRELQ
                     AFAQLSTDLDKATKDQLARGERMTELLKQNDLSPMPLEHQVVAIFAGNEGLLDDIPVE
                     KIKDFEKGLLDFIDKNCLDIFHEISEKKTISEALKEKMKEAILKFKNTFQ"
     gene            complement(3706..4245)
                     /gene="atpH"
                     /locus_tag="PK590_05085"
     CDS             complement(3706..4245)
                     /gene="atpH"
                     /locus_tag="PK590_05085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOG24005.1"
                     /translation="MNTTLVSVRYARALFELARQSGELASTDQWLTHFSEETKRYPKM
                     SSLMQNPIIATDEKNAFLEKLFPAGTPALLKNFLKVLVEKRRFLLFPEIRDIFHAMFE
                     RQQGILEVELLSATPFSEKLQEKLKQVLGLKLRSEIRIVPRTEPSLLGGFLLRFAGKE
                     IDCSFKNRFYEIEQQLLTC"
     gene            complement(4242..4742)
                     /gene="atpF"
                     /locus_tag="PK590_05090"
     CDS             complement(4242..4742)
                     /gene="atpF"
                     /locus_tag="PK590_05090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOG24006.1"
                     /translation="MEYFQENIRLGEVFVQLIAFLIVFFTLKKFAWKPLTNVIKSRRE
                     RFEQEWAGIEKMKTELATLETEYREHLQKIEETSRVKMQEAVQDGRRIAREIQEKARA
                     ESQASFEKAKENIELECEKARINLRREIAKLSLQIAEKVVREKMDPAQHEKKTLELIE
                     ELEGKL"
     gene            complement(4761..4991)
                     /locus_tag="PK590_05095"
     CDS             complement(4761..4991)
                     /locus_tag="PK590_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOG24007.1"
                     /translation="MTPETALGLSLPLGLAITAFSSAIALGKAVAAAMDATGRQPEAS
                     GKILVNMMAGCALIEALTLYSLVFGFVLMGKI"
     gene            complement(5021..>5601)
                     /locus_tag="PK590_05100"
     CDS             complement(5021..>5601)
                     /locus_tag="PK590_05100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOG24008.1"
                     /translation="TSFVTGILGPRGIEHVPFLGTLFIYVLFNNWLGLIPLMKSPTSA
                     WSTTIALALVTITYVHTIGIREQGFFNYLKHMAGNPQGAVGLALIPLMLIINIAVEYL
                     AVPLSLSLRLFANISSEDRLLHKFAELNVMYKFLPFFFQLFANILVIIFSLVQAFVFL
                     LLSTVYISLIMPHEDHNPKGTPQDQKLKAIHH"
BASE COUNT         1332 a         1367 c         1486 g         1416 t
ORIGIN      
        1 aatgctggag gattcgctgt acttctcgcg ccgtattgta atgatcctcc ccgacgattc
       61 gcgggtcaag tatgcgcgaa gttgaggcga ggggatcgac cgcgggataa atgccgagtt
      121 cgacaatttg gcgcgacaga accgtcaccc cgtcaagatg tgagaaagtg gtggccggag
      181 ccggatcggt aaggtcgtca gccgggacgt agatcgcctg aaccgaggta atagaaccgg
      241 ctttggtgga cgcgattcgt tcctggagct ccgccatttc agtcgcgagg tttggctgat
      301 aaccgacggc ggacggcatc cgcccaagaa gggccgagac ttccgctccc gcttgggtaa
      361 atcgaaaaac attatcgacg aacaccagaa catcttgatg ctcttcatca cggaaatact
      421 ccgccatcga gagaccgctt aaggccactc gaagtcgcgc cccgggcggt tcattcattt
      481 gtccgaagac catggctgtt ttgttaatga ctcccgattc cttgagctcc aaccacaaat
      541 cgttaccttc acgcgtgcgt tcgccgacac cgcagaaaac ggagacgcca ccatgctcgg
      601 tcgcgattga atggatgagc tccataacga ccacggtttt cccaacaccc gcgccgccga
      661 acagtccgac cttgcccccc tttgggtaag gagcgaggag atcaatgact ttaatcccgg
      721 tttcgagaat cgatttgacc ggaagcaatt cctcaaaaga aggcggcgcc cggtggatga
      781 catatcgttt ttccggattt tccaaaggcc ccgccttgtc gatggggtct cctaaaagat
      841 taaatacgcg gccgagcgtt tgatttccga caggaaccga aatcggtgct ttggtgttac
      901 gaaccggcat tccgcgaacc agcccgtcgg tggacgccag cgcgatacaa cggaccacgt
      961 tatcgccggt atgctgggcg acctcgagga cgagttttct cggctcggtg cccggaaaat
     1021 cgacctccag cgcgtccaga atagaaggca attgcccggc ctcaaactcg acgtcgacgc
     1081 tggggcccat aacttgaacg attttaccga atgacataac aactcctcaa aagttacatg
     1141 ctgcaagacc acatgccacg cgaattactc cgtaactttg tgatgtgtcc ttgcggctag
     1201 gtttttaaag cttttgatcc tgaaacgatt tcaatcaatt cattggtgat ggccgcctga
     1261 cggatcttgt ttcgcgttaa cgttagcgaa tcgatcagtt cccccgcatt ttgggtggca
     1321 agatgcatgg cccccatgcg ggcggtttgt tcggcgataa aagattccag aaaaaattcg
     1381 cggactttca cttcaaaaat catcgggata agccgctcaa aaagaaattc cggtgaaggt
     1441 tcaaaaatat aaggcaatga acgaacaggg tcctcgcttt ctttttggac cgaaaacgga
     1501 agaatttttt cagtgacggt tttatgagcg gattgggtca cgaattttgc ccgggtcacg
     1561 taaacggcgt ccacgacctg gtcgcggaaa agggtcccca taagaagtcc aatttcttgg
     1621 acggccgatt caacttcggt tgtttttgtg tccttaacca tcgtgtgcca ggtatggtga
     1681 tcacgcgaca aggcgttgat tccagctttg ccaaacccga tcaaaagggg tttcttggat
     1741 cgggattcaa tgaaccgccg ggtttcctgg atgagatcca tgttaaaaga tccgcacaat
     1801 cccgtatcgg atgtgactac caagagggcg cattgttttt cctcccgttc cgtaagcaga
     1861 ggatgatggc aactcgaaga agatcccaga atatttgcga gcagtttttc gagtgctcgc
     1921 gcgtacggag tcgtctggtc gagaagtttc tgaaaacgct tcagtttggc ggcggacacc
     1981 atttccatgg cgcgagtgat ttttttcgtg ctttcaatgc tccggatccg ggttttaatc
     2041 gtgcgtaatt ggccgctcat atttttctcg ggagacgggg aacagaaaac gcaagcgttt
     2101 ggcgttcccc ggtgcctcta ttgaaaagta tttttaaatt ttaagattgc ttccttcatt
     2161 ttttctttca atgcttctga aatagttttt ttctcggaga tctcatggaa aatatccaga
     2221 caattcttgt cgataaaatc gaggagaccc ttttcgaagt ctttgatctt ttcgacgggg
     2281 atatcatcca gaaggccttc attcccggca aagatggcga cgacctggtg ctcgagaggc
     2341 atggggctta aatcgttttg tttgaggagc tcggtcatgc gttcgccacg ggcgagctga
     2401 tctttggttg ccttatcaag atccgtggac agttgcgcga aagcctggag ttcacgatac
     2461 tgggcgagcg cgagtctgag cattcccgcg acctgtttca tcgctttggt ttgcgcgttt
     2521 ccccccacgc gtgaaaccga taatccgacg ttaatcgcgg ggcggacgcc ggcaaaaaag
     2581 aggtcaccct ccagatagat ctggccatcc gtaatcgaaa taacgttggt gggaatgtaa
     2641 gctgaaatat cgccaagctg ggtttcgatg accgggaggg cggtcatgga tccacctccg
     2701 agatcgtcgt gcagtttggc ggcacgttcc agaagccggg agtgaagata aaaaacgtct
     2761 ccggggaagg cttcgcgtcc cgggggtctc tgtagaagaa gcgagatctg acggtaggcc
     2821 gttgcgtgtt ttgacagatc gtcatagacc acgagcgcgt gttttcccga gcgcatggtt
     2881 tcttcgccga tcgcggcccc ggaataaggc gcgaggtatt gcaaaggcgc cggatcttcg
     2941 gatgaggccg atacaatcgt ggtgtattcc atggcaccgt atttttcaag agtgtcccgg
     3001 atggccacga cggtcgagag tttttggcca atcgcgacat agacacaatg aacccccgag
     3061 tctttttgat taataatcgc atcgatcagg atcgcggttt ttccagtctg gcggtcaccg
     3121 atcacaagtt cgcgctggcc ccggccaatc gggatcatgg agtcaatcgc tttgatgccg
     3181 gtttggagag gttccttgac gggctggcgc tcgacgacgg aaggaggaac tgtttcgatg
     3241 gggcgcattt tggtagaaat gaccggccct ttgccgtcga tggggtctcc gaggggattg
     3301 atgacccgtc ccttcaggcc ttccccgacc ggaacctgaa cgatttttcc cgttcttttg
     3361 acctgatcgc cctccttgat ttcccggtca gcgccgaaga tcacgactcc gacatggtgg
     3421 ggttcaaggt tgagcaccat acctgagact ccgttatgaa acagcaccag ctctccagcc
     3481 atggcttcat caaggccgta gacacgggcg atcccgtcac cgacctgaat cacgtaccca
     3541 acggattcaa gggacaacct agtttggtat ttttgaattt ctttttcaat aatttgcgtg
     3601 acttcttcgg gttttagcat aatttccttt gaaactcggg gtatggttta gaaggtaagg
     3661 atcgagcaag tttttcgcct gtccctttca acctatttcc cttttttaac aggttaatag
     3721 ttgctgttca atctcataaa aacggttttt aaatgaacaa tcgatttctt tgccggcaaa
     3781 gcggagcaaa aatccgccga gcagggaagg ttctgttcta ggaacgattc gaatctcgga
     3841 gcgaagtttt aacccgagaa cctgcttcag tttttcctgg agtttctctg agaacggagt
     3901 ggccgataac agttctactt cgagaatccc ttgctgtcgc tcaaacatgg cgtgaaaaat
     3961 gtcccgaatt tccgggaaaa gcaaaaagcg ccttttttcg acaagaacct tcaggaaatt
     4021 tttaaggagc gcgggcgtgc ctgccgggaa cagtttttcc aggaaagcat ttttttcatc
     4081 cgtggcgata atgggatttt gcatgagcga actcatcttt ggatagcgtt ttgtttcctc
     4141 cgaaaaatgg gttagccact ggtctgttga ggccagttct cccgattgtc tggcgagctc
     4201 aaaaagggcg cgcgcatagc ggacgctcac gagagtcgta ttcatagctt accctcaagt
     4261 tcctcgatca gttccagagt ctttttttca tgctgggccg ggtccatttt ttcccgaacc
     4321 accttttcag cgatctggag agaaagcttc gcgatttctc gccgaaggtt gatccgggcc
     4381 ttttcacact ccaactcaat gttttctttc gctttctcga aagaagcctg ggactcagcg
     4441 cgagcctttt cctggatttc acgcgcaatc cggcgaccat cttgcacggc ttcctgcatt
     4501 ttgacccgcg atgtttcctc gatcttttgc agatgttcgc gatattccgt ttccagggtg
     4561 gcgagctccg ttttcatctt ttcgattccc gcccattctt gttcaaatcg ctcccggcgg
     4621 gacttaatga cgttggtgag tggtttccag gcgaatttct tgagcgtgaa aaaaacgatc
     4681 aagaaggcaa tgagttgaac aaaaacctca cccagacgga tgttttcctg aaaatattcc
     4741 atggtccctg acgatcccga ctagatcttg cccatgagca caaaaccaaa aacaagcgag
     4801 taaagcgtca gagcctcgat cagggcgcaa ccagccatca tattgacaag aatctttccg
     4861 gaggcttcag gttggcgacc tgtcgcgtcc atagccgcgg ccacggcttt accgagcgca
     4921 atcgcggaac taaaggcggt gatggcgagg ccgaggggta gtgaaagtcc taaagcggtt
     4981 tctggcgtca taaaaaaaat ctccttctgc gtttttatgg ttagtgatga atggctttta
     5041 atttttgatc ttgaggcgtg ccttttgggt tgtgatcctc atgaggcata atgagcgaaa
     5101 tgtaaacggt tgaaagtaac agaaagacaa acgcctgcac aaggctgaaa ataatgacga
     5161 ggatgttagc aaaaagctga aagaaaaagg gaagaaattt gtacatgacg ttcagttcgg
     5221 cgaatttgtg gagaagacgg tcttctgagg agatgttcgc gaaaagccgg agggaaaggg
     5281 aaagtggaac ggctagatat tcgacggcga tattaatgat gagcattaaa gggatgagcg
     5341 cgagccccac ggctccctgc ggatttcccg ccatatgctt aagataatta aaaaatcctt
     5401 gttccctgat tccgatagtg tgcacatacg tgatggtgac cagggcgagc gcgatagtgg
     5461 tactccaggc gctcgtggga gatttcatga gcgggatcag tccaagccag ttgttaaaaa
     5521 ggacataaat gaagagagtt ccgagaaacg gcacgtgttc gatccctctg ggtccgagga
     5581 ttccggtgac aaaagaggtg a
//
LOCUS       DAOSIO010000113         5597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_13007_length_5597_cov_3.967340, whole genome shotgun sequence.
ACCESSION   DAOSIO010000113 DAOSIO010000000
VERSION     DAOSIO010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13007_length_5597_cov_3.967340"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PK590_05105"
     CDS             <1..639
                     /locus_tag="PK590_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HOG24009.1"
                     /translation="AKYGTSEVNWEKYKSLLSSKQSNVQRLCGEKLKEFSNLKDIVAG
                     AAKAEAFYRCAVELRLDSNYGKPKLLSNNKEYDGLALYNKGILEKIFNVENGIIFHNI
                     SQWCGLSQLEKGEDEFVKWYVKNGSQCAYCKSSILDLLRFYVKNTTKRNRGLSWEVDH
                     RKPDGSFCEENCALVCHYCNNAKSDIFKSSDEFRLIGNAIGQQIAEILTPAA"
     gene            804..1094
                     /locus_tag="PK590_05110"
     CDS             804..1094
                     /locus_tag="PK590_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24010.1"
                     /translation="MKKKHAIKKPKKIRGTAYELIGIMTIISCQAIERGLTPHAKWKN
                     TILDIYKDKKFIKKMRAAFAHNRDLSILARNFSTFIRVVERVGRGEGRRHGN"
     gene            1084..1941
                     /locus_tag="PK590_05115"
     CDS             1084..1941
                     /locus_tag="PK590_05115"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOG24011.1"
                     /translation="METNCVCFSGLLLKRYPQLFENLESALEQRGIPYGLISGTRDIW
                     CRDYMPIQVAEDRFVQFKYDPKYQRYKKYRHTITDVDAVCEAIGISPVKSKIRLDGGN
                     VVTLGSKAIITDRVFSENPGYGRKRLLAELKELLRVEWVIIIPECPGDMIGHADGLVR
                     FPHEVGSDNDTVYVSDLSSACPKYFPKLYSTLSQAGLTPILIPYDSPKKYDKMDATGT
                     YINYLQVGKFVFYPVFGSKADASAAKIFLRLFGQNAVPIRVGRLAKEGGVLNCISWAL
                     FRKGGRNES"
     gene            2341..2817
                     /locus_tag="PK590_05120"
     CDS             2341..2817
                     /locus_tag="PK590_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24012.1"
                     /translation="MDYETFVAFAERSKKLWQDFKDSFQEPELKAFLKNSYAVLSHPS
                     GQLYLWSTDFRTVAMESPGFLVPNQAKESLIKHVKELEALKKDSEERNKKERVRIEKW
                     FEEFDPQRLKFEQPRLEVRFPSLKMDLIQQIKQSKRVDQRFTGKERVAIRVVLLAP"
     gene            2930..4252
                     /locus_tag="PK590_05125"
     CDS             2930..4252
                     /locus_tag="PK590_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG24013.1"
                     /translation="MDSISDTINKALQQGYGFAEKLVEKNQTKFSRKQKELADFFGIR
                     QDQLSDLSTSKETLIRIYYRNAAFCFEPFVRENGRIVEEMNYDHYNGERISPVRQVIE
                     IEQDVRSKILERATVFFVWRGEKYCMEIEPKRWSSEGDYDLSFTSSDQSSLDALLEAF
                     KVYRQKHHYLRGKKFNGVSGDILPLNGYSWSDLMWPDGIKERLRSEIEVVFKNAALFH
                     SYGLNSKKGFILAGDPGNGKTLLLKILAKETAATVILIPFDELRFTPYLFSLARDLSP
                     SILILEDIDLFGTDRDNAAENVELGKLMNQLDGIVENREIVVIATTNRLEKVEKALQN
                     RPGRFDRIYVIPNPDLKMRISLIEHFIRKVPNEITQEHIEALAEEFPGYSAAYLMELV
                     NSGFAQAILRDSQNPVLRFCDLDATVDILKRKKDKIPVGFSVQAGVDK"
     gene            4254..4838
                     /locus_tag="PK590_05130"
     CDS             4254..4838
                     /locus_tag="PK590_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24014.1"
                     /translation="MKWLKSFRYAIYALLDRILGLAGYLEAVVAFRDSAIRSLECYVS
                     HLIEYFEEIPFLGAEEKNKEKLNAMGRLLDQANSFLVEIHGFPRKRLNDIERDLFCVM
                     MGGSDFYALRGDRLCAPKSIFDAKSVEIKHLLYMMHGGEVFMFRDLFIKAKVVKGRIV
                     FELDRDIIESRKLHEYEFSDDVREKVRTSKLSEK"
     gene            4856..4940
                     /locus_tag="PK590_05135"
     tRNA            4856..4940
                     /locus_tag="PK590_05135"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4890..4892,aa:Cys,seq:gca)
     gene            4979..>5597
                     /locus_tag="PK590_05140"
     CDS             4979..>5597
                     /locus_tag="PK590_05140"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOG24015.1"
                     /translation="MKIYKDFLEHILKNGVKRTERTGIGTLSVFGYQMRFDLRRGFPL
                     LTTKKMHWKSIVYELLWFLRGDTNVNFLKEHGVSIWNEWAGPDGDLGPVYGKQWRRWA
                     CPGGNSIDQIARAVELIKTDPDSRRIIVSAWNAGEIHQMKLPPCHMAFQFYVQNGELS
                     CQLYQRSADAFLGVPFNIASYSLLTHMVAHVTGLKPGEFIHSFGDA"
BASE COUNT         1524 a         1227 c         1467 g         1379 t
ORIGIN      
        1 gcgaaatatg gcacaagcga agtgaattgg gaaaagtaca agagcctgct ttcaagtaag
       61 cagtcaaatg ttcaacgctt gtgcggtgaa aaattaaaag aattcagcaa tttgaaagat
      121 attgttgctg gtgcggcgaa agcagaagcg ttttaccggt gcgcggtcga actgcggctt
      181 gattcgaact atggaaaacc aaagcttctc agtaataata aagagtatga tggtctcgct
      241 ctttataata aaggaatact agaaaaaatt tttaatgtcg aaaacggcat tatatttcac
      301 aacatatctc aatggtgcgg cttaagccaa ttggagaaag gggaagatga gtttgtgaag
      361 tggtatgtaa aaaatggttc ccagtgtgca tattgtaaaa gcagcatcct tgatttgcta
      421 aggttttatg ttaaaaacac aactaagaga aatcgtggac tatcttggga agtggatcat
      481 agaaagccag acgggtcttt ctgcgaagaa aactgtgctc tggtatgtca ttactgcaac
      541 aatgcaaaat ccgatatttt taagagttca gacgaattta gacttatcgg taacgctata
      601 ggacaacaga tagcagagat tttgactcct gccgcgtaaa ctgtcttgcc tgcggattag
      661 tacctcctta ttaagcgagt gttgcctttc tgaagaaaaa tctaccacgc gacaatagcc
      721 tgtcgttgtc gtggtgtatg atcctgccca cggaaatcaa gccgtcaaaa catcagcgcc
      781 tttgctctga tcacggagac cacatgaaga aaaaacatgc gattaagaag ccaaagaaga
      841 tccgtggcac cgcctacgaa ctcatcggca tcatgaccat catttcttgc caggccattg
      901 agcgcggtct tacgccacat gcgaagtgga aaaacaccat ccttgatatc tataaggata
      961 agaagtttat taaaaaaatg agggctgctt ttgcccacaa cagggatctg agcattctag
     1021 ccaggaattt cagcacattc atacgagtcg tcgaaagggt cggccgcggg gaagggcgca
     1081 ggcatggaaa ctaactgcgt ctgcttttcc ggacttctgc tgaagcgata tccccaacta
     1141 tttgaaaatc tggaatcagc gctcgagcag agaggaatcc cttatgggct catatcgggc
     1201 acacgcgata tctggtgcag ggattacatg cccattcagg tcgctgaaga tcgttttgtt
     1261 cagttcaaat atgatcccaa gtaccagcgc tataaaaagt accggcatac catcaccgat
     1321 gtggatgcgg tttgtgaagc gatcgggata agcccggtta agtcaaagat acggcttgat
     1381 ggcgggaatg tcgtgacttt gggatcaaag gcgatcatta cggatcgcgt attctccgag
     1441 aaccctggct acgggcgaaa gcggttgctt gcggagctga aagaattact ccgggtggag
     1501 tgggtcatta tcataccgga atgcccggga gacatgatcg ggcacgcgga tggccttgta
     1561 aggtttccgc atgaagtggg atctgataat gatacggttt atgtgagcga cttgtcctcc
     1621 gcctgcccca aatattttcc gaagctctac agcactctct cgcaggcagg tctgactccg
     1681 atcctgatcc cctatgacag tccaaagaaa tacgacaaaa tggatgcgac cgggacatac
     1741 atcaattacc ttcaggtggg aaaattcgtg ttctatccgg tattcggctc aaaagcagat
     1801 gcctctgcgg caaagatctt tttgaggcta tttggtcaga atgcggttcc gatacgggtc
     1861 ggcaggttag ccaaagaagg cggcgtgctg aattgcatca gctgggccct tttcagaaag
     1921 ggcggaagaa atgaatccta atacgaccgg aaatcctatt ctcgatgcga tcgggatatt
     1981 acttctgctg gggctattgg cattgcctgg cgccataggc gccgtaattt ggtatgccat
     2041 aaagatttgg tttactgcca agattgtaaa gaaagcctgg atgggtaagt agagaacaaa
     2101 gtctgttgtc tgcatgatgg cgggactctt cctgtttaga aaatagacca actgtcgaat
     2161 cctcaaaaaa aatcccgcaa gattcgctag cgacgcgacc ctgcccttgt ctttattgaa
     2221 ctatcgcacg agaaacagga ctaccctcgt ctttttgtgc atttttgaaa tagacgacga
     2281 ggggttgtcg caggggtttt gtagcattct cctcccaaat tcaaaccacg ggaggagaag
     2341 atggattatg aaacttttgt ggcgtttgcc gagcgctcga aaaaactctg gcaggatttc
     2401 aaagacagct ttcaagaacc ggaacttaaa gccttcctca aaaattccta tgcagttttg
     2461 agccatcctt ccggacaact ctacctttgg agcacggact tccggacggt cgcaatggag
     2521 tctcccggct ttctagttcc gaaccaggca aaagagagcc ttatcaagca cgtcaaagag
     2581 cttgaggcgc tcaagaaaga ttcggaagag cggaacaaaa aagagcgggt ccggatcgag
     2641 aagtggtttg aggaatttga tccccaaagg ctgaagtttg aacaaccacg gcttgaggtc
     2701 aggtttccgt ctctaaaaat ggacctcatc cagcagatca agcaaagcaa gcgcgtagat
     2761 cagcgtttta cgggcaagga aagggttgcg atccgggttg tgcttttagc gccctagata
     2821 gaccctaccg gttgtgtctg ccaaatactt atccgctaga tacacacgcg acgcagtgct
     2881 gtcgcatgcc gggagtatca tctctcataa agttaacggg agaagagata tggattcgat
     2941 cagtgatact atcaacaaag cgcttcagca aggctatgga ttcgcggaaa aacttgtcga
     3001 aaaaaatcag accaagtttt cgagaaaaca aaaggaactg gccgactttt ttgggatccg
     3061 gcaggaccag ctttcagatc tctctacctc gaaagagaca ttgatcagaa tttactaccg
     3121 gaatgcggcc ttctgcttcg agccgttcgt ccgggaaaac ggccggatcg ttgaggagat
     3181 gaactacgat cactataacg gagaaaggat ttctccggtc aggcaggtta tcgaaatcga
     3241 gcaggatgtc cggagcaaga tcctggagcg ggccaccgtc ttttttgttt ggaggggaga
     3301 gaagtactgc atggaaatcg aacccaagcg ctggagctcc gaaggagact atgacttatc
     3361 gtttacttca agtgatcaaa gcagcctcga cgcgctgttg gaggcattca aggtttatcg
     3421 tcagaagcat cactacctcc gggggaaaaa gtttaatggt gtgtccggag atattcttcc
     3481 gctcaacgga tatagctgga gcgacctcat gtggccggac ggtatcaagg agcggctacg
     3541 tagcgagatc gaggttgttt tcaagaatgc ggctctattt cattcgtacg gtctgaacag
     3601 caaaaaagga tttattcttg cgggggaccc cggaaacggg aagactcttc ttttgaagat
     3661 ccttgccaag gagaccgctg cgaccgtcat cctgatcccg tttgacgagc tcagatttac
     3721 accctatctc ttttctctcg caagggatct ttcaccgtcc attttgatcc ttgaagatat
     3781 tgatctcttc ggtactgacc gtgacaacgc cgcagaaaat gtagagctcg ggaagctgat
     3841 gaaccagctg gatggaatag ttgagaatcg cgagatcgtt gtgatcgcta cgaccaacag
     3901 gcttgagaag gtcgaaaagg ctctccagaa tcgtcccggg cgttttgacc gcatttatgt
     3961 gatcccgaac ccggatttaa agatgagaat cagcctgata gaacatttta tcaggaaggt
     4021 tcccaatgag atcacgcaag agcatatcga agccctggct gaggaatttc cgggctattc
     4081 ggcggcgtat ctgatggagc tcgttaattc ggggtttgcc caagccattt tgagggacag
     4141 tcaaaacccg gttctgaggt tctgcgatct tgatgctacg gtggatattc tcaagcgcaa
     4201 gaaagacaag attccggtcg gtttttcagt acaagcggga gtggataaat gacatgaagt
     4261 ggcttaaaag ctttagatat gccatttatg cgttgctgga tcgcattctt ggcctggctg
     4321 gctatttaga agcggttgtt gccttcaggg atagcgcgat ccgctcttta gagtgctatg
     4381 tttctcatct catcgagtat tttgaggaaa tccctttctt gggtgccgaa gaaaaaaaca
     4441 aagaaaagct gaatgctatg gggcggcttt tggaccaggc gaattctttc ttggtggaaa
     4501 tccatgggtt tccgcgcaag cgcttgaatg atattgagag ggatctcttt tgcgtgatga
     4561 tgggaggtag cgatttttac gccctaaggg gagaccgtct ctgcgctccg aaaagcattt
     4621 tcgacgcaaa gtccgtggaa atcaaacatc tcctttacat gatgcatggt ggggaagtat
     4681 ttatgtttcg ggatctattc attaaagcaa aggtcgttaa agggcggatc gtattcgaac
     4741 ttgatcggga tatcatcgag agccggaaac tgcacgaata cgaattctcg gatgatgttc
     4801 gggaaaaagt gagaacttca aaactaagtg aaaaataaaa accgggtgca ttttagacgg
     4861 gatggccgag tggtcgaagg caaaggactg caaatccttc cggagtgatc cgcacgcggg
     4921 ttcgaatccc gctcccgtct cgaatcatgc cgaatcaaat ctttgagcgg gagtccaaat
     4981 gaaaatttat aaagactttt tagagcacat ccttaaaaac ggcgtcaaga gaacggagcg
     5041 gacgggcatc ggaacgctta gcgttttcgg gtatcaaatg cggtttgacc ttcgccgtgg
     5101 atttccgctg ctgacaacca aaaaaatgca ctggaagtcc atcgtttatg agcttctctg
     5161 gttcctgcgg ggggatacga acgtaaattt cctgaaagaa cacggcgtta gcatttggaa
     5221 tgaatgggcc ggccccgacg gggatctcgg gcctgtctat ggaaaacaat ggcgcagatg
     5281 ggcgtgcccc ggcgggaatt ccatagatca gatcgcgcgg gccgttgaac taatcaaaac
     5341 agaccccgac agccggcgaa tcatcgtgag tgcgtggaat gccggcgaga tccatcaaat
     5401 gaaacttccg ccgtgccata tggcgttcca gttttatgtc cagaacgggg agctatcctg
     5461 tcagctctat cagcgcagcg cggacgcatt tctgggggtc cctttcaata tcgcgtcgta
     5521 ttcgcttttg acgcacatgg tcgctcatgt gaccggcctc aagccgggcg aatttattca
     5581 ttcattcggg gacgcgc
//
LOCUS       DAOSIO010000114         5592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_13020_length_5592_cov_3.513636, whole genome shotgun sequence.
ACCESSION   DAOSIO010000114 DAOSIO010000000
VERSION     DAOSIO010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13020_length_5592_cov_3.513636"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="PK590_05145"
     CDS             <1..1108
                     /locus_tag="PK590_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HOG24016.1"
                     /translation="CRLAEQKHGTIDWSLREKQLVELCDRHRKHDGSYDCLVPGSGGK
                     DSFYAAHILKYKYGMHPLTVTWAPHVYTDWGWKNMQSWIHAGFDNYLCTPNGRVHRLL
                     TRLAVENILHPFQPFIIGQKALAPKMAILFNVPLVFYGENEAEYGNPISDTLTAKRDW
                     SYFTASDKSKIYLGGTSVADLSIHYGVDENDLKVYLPADPEEIERQKVEVHYLGYYLK
                     WHPQSCYHYAVEHGGFQASPERTPGTYSKYNSIDDRIDDYHYYTTYIKFGIGRATYDA
                     AQEIRSRDITRDEGVALVKRFDGEFPERFAEEIFRYLSITEKEFPVASKMFKHPVMDR
                     QYFMHLCDQFRSPHLWKKEAGEWKLRHAVWMEKA"
     gene            1105..1248
                     /locus_tag="PK590_05150"
     CDS             1105..1248
                     /locus_tag="PK590_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24017.1"
                     /translation="MTRNVCLIDQLDVRGPNLTNRIYLEGLRVMAVQVCLHTKTMNKA
                     LMN"
     gene            1254..1535
                     /locus_tag="PK590_05155"
     CDS             1254..1535
                     /locus_tag="PK590_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOG24018.1"
                     /translation="MDTVANLYNLNSLFEIVRTTARNVFIPIALDGRIPSIKDVTKLL
                     QARANKAIINTVAIRSLNCYGAQFHPGKSGPVGLGLRRNFLVLSRGGSQ"
     gene            1547..2524
                     /locus_tag="PK590_05160"
     CDS             1547..2524
                     /locus_tag="PK590_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24019.1"
                     /translation="MIRPQELARDIVVIDGMSCAGKSFVAPLLSSLDRGELWTINHLY
                     EYLCTMYHYGRMSLDAASTLVQLYADLDLYNLRVGRNTNFRRSDISSAHFNLQGTRYH
                     ARTRLKDGDGIVREIFRARPILFLMTHYIFGQSDLLFKAFGSRLKFYLIVVRHPLDLL
                     ESWYKGNWDCRVGSDPREFQLCCAVKGQKAPWFATPWLRQYRRLNRLEKSVATVVWFQ
                     KEFKRQWKRLSPEERRKVIFLPFEKVAPDPVTALCGIAKKLGAKITPLTGRVMKKFCM
                     PRNLDPEHISLQYRKFSNAFKLRGVRRGIADEFWKTCEHYEITHIQESR"
     gene            2528..3292
                     /locus_tag="PK590_05165"
     CDS             2528..3292
                     /locus_tag="PK590_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOG24020.1"
                     /translation="MSSLKSKLRSGKAIYGSWLTLAHPGISEIMASAGFDFLVVDMEH
                     SAIDLSQAQMLVQSIQAQGVAALIRVGDQDPNRIKRAMDTGVDGVIVPMIKNRAEAEA
                     AVAAVHYPPHGTRGVGLARAQKYGFGFEGYKRWLRKEGVVIGIIEHHEAIENLEDILG
                     VDGLDGTMIGPYDLSGSLGFPGEFDRPVVRKLIDRYESVCRRMKKPMGFHVVHPDTKR
                     VLDYQKRGYKFLAVGLDSLYLGLKSRETMKQIRRGR"
     gene            3289..4062
                     /gene="kdsB"
                     /locus_tag="PK590_05170"
     CDS             3289..4062
                     /gene="kdsB"
                     /locus_tag="PK590_05170"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011369350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOG24021.1"
                     /translation="MNIIGIIPGRMASSRYPGKPLAKIAGIPMIGHCYFRSKMSQRLN
                     EVYIATCDKEIADYGDAVGARCVMTKDTHERATDRIAEAVDKIEAQTGKRVDIVVMIQ
                     GDEPMTQPGMIDEAVEPLLKDNSIVIANLMAPIMSREEHDDPNEVKVVVDRQGFALYF
                     SREPIPSWRKGAKEVPMLKQVCVIPFRRDFLKTFITLSPTPLEIIESVDMLRAMEHGY
                     KVKMVMTKFESYSVDTPEDLQFAETYLLKDALVAKYPKC"
     gene            4078..5064
                     /locus_tag="PK590_05175"
     CDS             4078..5064
                     /locus_tag="PK590_05175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG24022.1"
                     /translation="MKEEEIRKRSVFNRYLELLSYDIRTFFKDWSRFIPSRCPACDSD
                     KRITEFTKEGFTYVACTECDTLYVNPRPPFLELMRFYTDSPSTHFWVNDFFKPVAEIR
                     REKIFKPRVDFITKWFGDQPLDAIGDIGAGFGLFLEELKKVWPAKRLVAIEPSTEMAA
                     ICSGKGLEVIPKAIEDVTEGKRSFDLLTAFELFEHLHEPGCFLRTARELLKPGGYLLL
                     TTLSGHGFDIQTLWERSKSVQPPHHLNFFNPKFMELLLKRCGYEVIEISTPGKLDWDI
                     VEGMILQEGARPGRFWELFARSGEPQAKVALQEWITQNNLSSHLRTIARRPR"
     gene            5078..>5592
                     /locus_tag="PK590_05180"
     CDS             5078..>5592
                     /locus_tag="PK590_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG24023.1"
                     /translation="MTKIAITTSSFAKDKHDVLKMLPADCEVIMNPYGRTLKPDELVQ
                     LAGEAIGIIAGTEKYDATTLSQLKNLKVISRVGVGIDGIDLAVAKERGIKVLNTPNGP
                     TQAVAELVLGLMLSLLRKTHLMDRELRQGNWKKEMGNLLCSKAVGIVGLGRIGRKVAE
                     LVSAFGAKVGYS"
BASE COUNT         1361 a         1362 c         1518 g         1351 t
ORIGIN      
        1 ctgtcgtctt gctgaacaga aacacgggac gatcgactgg tcgcttcggg aaaaacagtt
       61 ggtggagctt tgcgatcgtc atcgcaaaca tgatggctcc tacgactgcc ttgtcccggg
      121 gtcaggcggt aaggatagct tttatgcggc gcatattttg aaatacaagt acgggatgca
      181 ccccttgacc gtgacttggg cgcctcatgt ttataccgat tggggatgga agaacatgca
      241 gtcttggatc cacgcgggat tcgacaatta tctttgcacg ccgaacggtc gtgtgcatcg
      301 acttctgacg cggctggccg tcgagaacat ccttcatccg ttccagccgt tcatcattgg
      361 tcagaaggca cttgcgccga aaatggcgat tctcttcaat gttcctctcg tgttctacgg
      421 tgagaatgag gcggaatatg gcaacccgat cagcgacact ttgactgcaa aacgcgactg
      481 gtcgtatttc acggcttctg ataaatctaa gatttatctc ggtggaacct ccgttgccga
      541 cctgagcatc cactacggcg tcgatgagaa cgacctcaag gtctacctac cggcggatcc
      601 ggaagaaatc gagcgccaga aggttgaggt ccattatctc ggctactatc taaagtggca
      661 cccgcaaagt tgttatcact atgccgttga acacggcggt ttccaggcct cgccagaacg
      721 tactcctggc acctacagca agtacaacag tatcgatgac cggattgatg actatcacta
      781 ttacacgacc tacatcaagt ttgggattgg tcgtgcgacc tatgatgcag ctcaggaaat
      841 tcgctcgcgc gacatcactc gcgatgaagg tgttgcgctg gtcaaacgtt ttgacggtga
      901 attcccggag cgtttcgccg aggagatctt ccggtatttg agtattacgg aaaaagaatt
      961 ccctgtcgca agtaaaatgt ttaaacatcc ggtcatggac cgtcaatatt tcatgcacct
     1021 ttgcgaccag tttcgttcgc cgcacctttg gaaaaaggag gcgggggagt ggaaactgcg
     1081 tcacgccgtg tggatggaga aagcatgacc aggaatgtct gtctgatcga tcagcttgat
     1141 gtaagagggc cgaatctgac aaacaggatc tacttggaag ggctcagggt gatggcggtc
     1201 caagtttgtt tacacaccaa gactatgaat aaggcgctga tgaattgatc tgtatggata
     1261 cggttgcgaa cctttataac ctgaacagtc tgttcgagat cgtccgcacg accgcgcgga
     1321 atgtttttat tccaattgcg ctcgacggaa ggattccttc gataaaggac gttacaaaac
     1381 tgcttcaagc cagagcgaat aaagccatca tcaatacggt tgcaattcga tccctgaatt
     1441 gctatggagc gcaatttcat cccgggaaaa gcggccctgt aggcctcggc ctgaggcgta
     1501 actttctcgt actctcgcga ggaggttccc aatgagaggt gtccggatga tacggcctca
     1561 agagcttgcg cgcgatatcg tcgtgattga tggtatgtcg tgcgccggca aatcttttgt
     1621 cgcgcccctc ctgagctcgc tcgatcgcgg tgagctttgg acgatcaatc acctctatga
     1681 atacctttgc accatgtatc attacggccg tatgtcgtta gacgccgcgt caacgcttgt
     1741 ccagctttac gcggatctcg acctttataa tttgcgtgtt ggccgtaaca cgaatttcag
     1801 gcgatcggac attagcagtg ctcacttcaa tcttcagggt acccgctatc atgcacggac
     1861 ccgcctcaaa gacggggacg ggattgttcg cgaaattttt cgtgcccgtc cgatcctctt
     1921 tttaatgacc cactatatct ttggtcaatc ggaccttctt tttaaggcgt ttggaagccg
     1981 gctcaagttt tatcttattg tagtccgaca cccgctcgac cttcttgaaa gctggtataa
     2041 ggggaactgg gactgccgag ttggttctga tccccgcgag ttccagctct gttgcgccgt
     2101 caaagggcag aaagcgccct ggttcgctac cccgtggctc cgccaatatc gtcgcttgaa
     2161 ccgccttgaa aaaagtgtcg cgacggtcgt ctggttccag aaggagttca aacgtcaatg
     2221 gaagaggctt tctccggagg agcgtcggaa ggtgattttc cttccgtttg aaaaagttgc
     2281 tccggacccg gtgacggccc tttgcgggat cgccaagaag cttggcgcca aaataactcc
     2341 cctgaccggc agggttatga aaaaattttg tatgcccagg aaccttgacc cggaacacat
     2401 aagtctgcag tatcggaagt tcagcaacgc attcaagttg cgaggggttc gtcgtgggat
     2461 cgcggatgaa ttttggaaga cctgtgaaca ctacgagatt actcacattc aggaaagcag
     2521 gtgaatcatg agctcactta aatcaaaact acgatccggc aaggccattt acgggagctg
     2581 gctgacgctt gcccatcccg gcatttcgga gatcatggct tcggccggat ttgatttttt
     2641 ggtggtggat atggagcact cggcaattga tctttcgcaa gcgcaaatgc tcgtgcaaag
     2701 tatacaggct caaggtgttg ccgcattgat ccgcgtcggg gaccaggacc caaaccgcat
     2761 caaacgggcc atggatacgg gcgttgacgg agtaatcgtt ccgatgatca agaatcgcgc
     2821 tgaagctgag gcggccgtgg ccgcggttca ctatcctccc cacggaactc gcggtgtcgg
     2881 tctcgcgcgg gcccagaagt atggattcgg atttgaagga tataaaagat ggctccggaa
     2941 ggagggtgtc gtcattggga tcatcgagca tcatgaagcg atcgaaaatc ttgaggatat
     3001 ccttggcgtt gatggccttg acgggaccat gatcggtccc tatgacctct ccgggtcgct
     3061 cggatttccc ggtgaattcg accggccggt cgttcgtaaa ctgatcgacc gctatgagtc
     3121 ggtctgtcgc cggatgaaaa agccgatggg ctttcatgtt gtgcatccgg acacgaaaag
     3181 agtgctggac taccaaaaac gcggttataa atttctcgcg gtcgggctgg acagtcttta
     3241 tctgggattg aagtcccgcg aaaccatgaa gcagatcagg agggggagat gaacattatt
     3301 ggtattattc cgggacgaat ggcttcgtcc cgttatcccg ggaaacctct ggcgaaaatt
     3361 gccggcatcc cgatgattgg gcactgctat tttcgttcca aaatgagcca gcggctgaac
     3421 gaggtctaca tcgccacctg tgacaaggaa atcgcggatt acggtgacgc ggtcggagcg
     3481 agatgtgtga tgaccaagga cacgcatgag cgcgcgaccg accggatagc ggaggcggtg
     3541 gacaagattg aagcgcagac tggaaaacgg gtcgatattg tggtgatgat ccagggcgat
     3601 gaaccgatga cgcaacccgg catgattgat gaagccgttg aaccgttatt gaaggataat
     3661 tcaattgtta tcgcgaatct tatggcaccg atcatgtccc gggaagagca tgatgatcca
     3721 aacgaagtga aggtcgttgt ggatcggcag ggcttcgccc tgtatttttc acgcgagccg
     3781 atcccctcgt ggagaaaagg ggcaaaagag gttcccatgc tcaagcaggt gtgcgtgatc
     3841 ccgtttcggc gagatttcct taaaacgttt atcactttat ctcccacgcc gcttgaaatt
     3901 atcgaatcgg tggatatgct tcgtgcgatg gaacatggtt acaaggtcaa gatggtcatg
     3961 accaagtttg aaagttacag cgtcgacacg ccggaagatt tgcagttcgc ggaaacgtat
     4021 ctgctaaaag atgcgttagt cgctaaatac ccgaaatgct agacggaggc ctcaggaatg
     4081 aaagaagaag agatcaggaa acgcagtgtt tttaaccgct acctggaact tttaagctac
     4141 gatatccgca cgttcttcaa ggactggagt cggttcattc catcgcgctg ccccgcgtgc
     4201 gattcagaca agcgtatcac tgagtttacg aaagaaggat tcacttatgt tgcctgcact
     4261 gaatgtgaca ctctgtatgt caatccgcga cctccattcc tggagctcat gcgcttttac
     4321 accgattcgc cctcgaccca tttctgggtg aatgattttt tcaaacccgt cgcggagatc
     4381 cgccgcgaga agatctttaa accgcgcgtc gacttcatca caaaatggtt cggcgatcag
     4441 cctcttgacg cgatcgggga tattggtgcc gggttcggtc ttttccttga agagctcaaa
     4501 aaagtctggc ctgccaaacg tcttgtcgct atcgaaccgt ccacagaaat ggcggctatt
     4561 tgttccggga aggggcttga agtcatcccc aaggcgattg aagatgtcac cgagggaaaa
     4621 cggtctttcg atcttctcac tgcgttcgag ctttttgaac atcttcatga accgggctgt
     4681 ttcctgagaa ccgcacggga actacttaaa cccgggggat acctcctgct tacaacgttg
     4741 agtggacatg gatttgatat tcaaaccctc tgggaacgct ccaaaagcgt gcaaccaccg
     4801 caccacctaa atttttttaa tcccaagttc atggaactcc tgctgaaacg ttgcggctat
     4861 gaggtgattg aaatatcgac gccgggcaaa ctcgattggg atattgtcga aggtatgatc
     4921 ctccaggaag gtgcccggcc gggacggttc tgggaactat tcgcgaggag cggcgagccg
     4981 caggcgaagg tcgcattgca ggaatggatc actcaaaata atctcagctc tcatttgcgc
     5041 accattgcca gacgaccgcg ttgaaagggg gcaaaccatg accaaaatcg caatcacgac
     5101 aagttcattt gcgaaagata aacacgacgt tctcaaaatg cttccggcag attgcgaagt
     5161 aataatgaac ccgtacggtc gaaccctgaa acccgatgaa ctggttcaac tcgccggaga
     5221 agcaatcggt atcattgctg gtacagaaaa atacgacgct acaacccttt cacaattaaa
     5281 gaatctgaag gtgatctcga gggttggtgt cgggattgac gggatcgatc ttgccgttgc
     5341 caaagagagg gggatcaagg ttcttaatac gcctaatggt cccacgcagg ccgttgctga
     5401 gctggttctc ggtttaatgc tgagtctcct tcgcaaaact catttaatgg atcgcgaact
     5461 acgtcagggg aactggaaaa aagaaatggg aaatcttctt tgttcgaagg ctgtcgggat
     5521 cgtcgggttg ggacgtattg ggcgaaaagt cgcggaactc gtgagcgcct ttggcgccaa
     5581 ggttggctac tc
//
LOCUS       DAOSIO010000115         5476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_13361_length_5476_cov_4.694521, whole genome shotgun sequence.
ACCESSION   DAOSIO010000115 DAOSIO010000000
VERSION     DAOSIO010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13361_length_5476_cov_4.694521"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PK590_05185"
     CDS             <1..263
                     /locus_tag="PK590_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG24024.1"
                     /translation="IKKLSEKEYDLVFLDSSMPWMTGDKVFEKIRQISEVPVVFISGF
                     ISPEKKRKVMAMGAVSCLEKPLDVKHVKSLAHSIIRRNFNTN"
     gene            281..1564
                     /gene="gltX"
                     /locus_tag="PK590_05190"
     CDS             281..1564
                     /gene="gltX"
                     /locus_tag="PK590_05190"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOG24025.1"
                     /translation="MSNIRVRFAPSPTGYLHIGGVRTALFNFLYARQQGGKFLLRIED
                     TDRLRSTPEFEQEILDSLKWLGLDWDEAIARQSNRLDRYREVARELTEKGFAYESEGA
                     IRFRMPKDQKVIFDDIVKEKVEYPTADFEDLVIMKSDGFPTFHLAVIVDDHDMGVTHV
                     IRGDDHLPNTPRHILIQRALGWKSPRYGHLPLILGADGTPLSKRHGAVAVTEYRKQGF
                     LPEGLLNYLALLGWNPGDNQEIFTLQELIKRFSLKRIIKSAACFNPEKLLWLNGQHIR
                     KIRPEDYETRITEFWKGKGSLPEERKWRKLIALYRPRIETLGQLMDQASYCFSEPDAK
                     DHPMMGEIKANKPVMDLLRQLRGQLEELEDFEDVSLLDQKTKQVAESAGFKPKDIIHP
                     LRFVLTGGTASPGIFELMSLLGKETCLKRLSSFFL"
     gene            1754..3448
                     /locus_tag="PK590_05195"
     CDS             1754..3448
                     /locus_tag="PK590_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOG24026.1"
                     /translation="MEPQKKHSNFIRDIIDRDLANGKNGGRIHTRFPPEPNGYLHIGH
                     AKAICISFGIARDYRGLCNLRFDDTNPEKEESEYVNSIREDVRWLGFDWGDREYYASD
                     YFDSLYQHAETLIQKGKAYVCDLTADEQREYRGTLTRPGKNSPYRDRPVEENLDLFRR
                     MRAGEFPDGARVLRAKIDMASPNMNMRDPTMYRIKRVSHHRTGDRWCIYPMYDFTHCI
                     SDSIEGITHSLCTLEFENNRPLYDWFLVELGIHRPQQIEFARLNLTHTVLSKRVLLRL
                     VQQKIVSGWDDPRMPTISGFRRRGYTPSSIRNFCDQIGVAKFNSTIDMGVLENSIREE
                     LNKTAPRMMAVLKPLKVVITNYPEGKTEELEAVNNPEDPSAGSRKVPFGRELYIEQED
                     FREAPPPKYFRLAPGKEVRLRYAYFIKCEKVIKDPATGDVKEVHCTYDPETHGGNNPP
                     DGRKVKGTIHWVSAKHAVSPEVRLYEPLFNVKDPNDFPEGKDIFVNMNPQSLEVLKHA
                     KLEPALGQMAPGERVQFERMGYFFVDPKDSRPGQPVFNRIVTLRDTWGKIEQKRLA"
     gene            3588..3658
                     /locus_tag="PK590_05200"
     tRNA            3588..3658
                     /locus_tag="PK590_05200"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3620..3622,aa:Gln,seq:ctg)
     gene            3983..4360
                     /locus_tag="PK590_05205"
     CDS             3983..4360
                     /locus_tag="PK590_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG24027.1"
                     /translation="MGKKILVIDDEELITKSLVLALEKNGYEVLVAKRCDDALAMAEA
                     IDFDLIISDIRIPGVDGIETVRRILDLMATRKAKRAPRIFMTGYADPLTEENAQTLKP
                     SAYILKPFDLLEFLTEVKKALGE"
     gene            4364..5383
                     /locus_tag="PK590_05210"
     CDS             4364..5383
                     /locus_tag="PK590_05210"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG24028.1"
                     /translation="MPNIDILLKNQEISLLSKIKKYRKLAKEIKNHLDRDPDSWGQYQ
                     SVFNQEINAIFRDLMLFEKERLAEGDDVSVYKLKKMFIKKLRADFLYGEHIRWSLEKP
                     FGYAGDYKIIDDMYRNDPQTNGFERLFDNYSQMSPAANAIRNRKQDIKKFISTVFKSR
                     KGDCLKVMDLASGPCRDIKELLDELSPAAGQISIDCFDQDQGAIDYAKNLLRDYPEIV
                     TFYRQNAVRFALKKDVPQGKYDLIFSLGLFDYLDERIARHLIAALKKQLKKNGLLFIA
                     NFGEQYQNPSFYFLEWVGEWQLIYRSIEALKKIFLMAGFSDKGLRSESEQQGIIHYVI
                     AKNPG"
BASE COUNT         1428 a         1246 c         1391 g         1411 t
ORIGIN      
        1 gtatcaagaa actttccgaa aaggaatatg accttgtgtt cctcgattca agcatgccgt
       61 ggatgacggg cgataaggtt tttgaaaaga tccggcaaat cagcgaggtt cccgtcgtgt
      121 ttatttcagg gtttatcagt ccggaaaaaa agcgcaaggt aatggctatg ggggcggttt
      181 catgtctcga aaagccgctg gacgttaaac acgtgaaatc tctggcccat tcgattatca
      241 gacgcaattt taatacaaat tagaaacctg attgaatcct atgtcaaaca tccgagttcg
      301 ttttgcccct tccccgaccg ggtatcttca tatcggaggt gtccggaccg cgctttttaa
      361 ttttttatat gcccgccagc agggagggaa atttcttctt cgcatcgagg acacggatcg
      421 gcttcgttca actccggagt ttgaacagga gatcctggat tctctcaagt ggcttggact
      481 ggactgggac gaggcgattg cgcgccagag caaccggctg gaccggtatc gggaggtcgc
      541 cagggaactc accgaaaagg gtttcgctta cgaatcggaa ggcgcgatca ggttccggat
      601 gccaaaagat cagaaagtta tttttgacga tatcgtaaaa gagaaagtcg aatatcccac
      661 cgcggatttt gaagatctgg tcattatgaa gtctgacggt ttcccgacct tccatctcgc
      721 ggtgatcgtt gatgaccacg acatgggagt gacccatgtg attcgtggtg acgatcattt
      781 accgaatact ccgcgtcaca ttttgatcca gcgggcgctt ggatggaaat ctcccaggta
      841 cggacacctg ccactgattt tgggcgcgga cggaacgccg ctttccaagc gccacggggc
      901 cgtcgcggtt acggaataca ggaaacaggg ttttcttccg gaaggacttt tgaattatct
      961 ggcgctcctg ggctggaatc cgggtgataa ccaggaaatt ttcactttac aggaactcat
     1021 caagaggttt tcgctgaaac ggattattaa gtccgcggcg tgtttcaatc cggaaaaact
     1081 tttatggctg aacggacaac acattcgcaa aatccggccg gaagattatg aaacgcggat
     1141 cacggaattt tggaagggga aggggagttt gcccgaagaa aggaaatggc ggaagcttat
     1201 cgcgttgtat cgtccgcgaa tcgaaacatt gggacaactg atggatcagg cgtcgtactg
     1261 tttttccgag cccgacgcca aagaccatcc gatgatgggt gagatcaagg cgaacaagcc
     1321 ggttatggat cttcttcgcc agcttcgggg ccagctcgag gaactggaag actttgaaga
     1381 cgtttcgctt cttgatcaga agacaaagca agtggctgaa tccgcggggt ttaaaccgaa
     1441 agatatcatt catccgctcc gtttcgtcct gaccggagga accgccagcc caggtatttt
     1501 tgagcttatg agcctccttg gaaaagaaac ctgcctgaag cgcctttcct cgttctttct
     1561 ttaaaatcgc aggctcggct cttcagagaa tctgatataa tcgtggctcg acagctttgg
     1621 aagatgtctc gcctgtttta tcgaaaaatt cttcttgtca aagagacgag cttttaattt
     1681 taaatagccg ggagcggttg cttttaaggg ccgggggccg ttcccgattt ttgtcatcat
     1741 ggagacgtaa agagtggaac ctcaaaaaaa acattcaaat tttatccggg acatcattga
     1801 ccgggatctc gcgaacggta aaaacggcgg gcgaatccat acccgtttcc cgccggagcc
     1861 gaacggctat ctccacatcg gccacgccaa ggcgatttgc atcagttttg gaatcgcccg
     1921 ggattaccgg gggctttgca atctgcggtt tgatgatacc aatcccgaaa aagaagaaag
     1981 cgaatatgtg aactcgattc gggaggatgt ccgctggctt gggttcgact ggggagatcg
     2041 ggaatattac gcttcggatt attttgactc tctctatcag cacgcggaga cccttattca
     2101 aaaggggaag gcctacgttt gcgatttgac ggcggatgaa cagcgggagt atcgggggac
     2161 gttgacccga cccggtaaaa acagtcctta ccgcgaccgc ccggtcgaag aaaatctgga
     2221 tcttttcagg cggatgcgcg caggcgaatt ccccgacggg gctcgtgttc ttcgcgcgaa
     2281 gattgatatg gcttccccca atatgaatat gcgggatccc acgatgtatc ggatcaaacg
     2341 ggtctcgcat caccggaccg gtgaccggtg gtgtatttat ccgatgtatg attttactca
     2401 ctgcatttca gattcgatcg agggcattac gcactccctc tgcacgcttg aatttgaaaa
     2461 caatcggccg ctttacgact ggttcctggt ggagctggga attcatcggc cccagcaaat
     2521 cgagtttgcc cggctcaatc tgacccatac cgttttaagc aaaagggttc ttctcaggct
     2581 tgtccagcag aagattgttt ccggctggga tgacccgaga atgcccacaa tctcgggatt
     2641 tcgccggaga ggttacacgc cctcgtccat tcgcaatttt tgtgaccaga tcggcgtcgc
     2701 caaatttaac agtactattg acatgggggt gctcgaaaat tcgattcgtg aagagctcaa
     2761 caaaacggcg ccgcgcatga tggcggtgct taaaccgctc aaagtggtga tcacaaatta
     2821 tccggaaggg aagaccgaag agcttgaagc tgtgaataat cccgaagatc cttccgcggg
     2881 atcacgcaaa gtaccgtttg gtcgtgagct ttacattgaa caggaggatt tccgtgaggc
     2941 gccgccgcca aaatatttcc gcctggcgcc cgggaaagaa gttcgtttgc gttacgctta
     3001 cttcatcaaa tgtgaaaagg tcatcaagga ccccgcgact ggtgacgtga aggaggtcca
     3061 ttgcacctat gatccggaga cgcacggcgg caataaccct ccggacgggc ggaaagtgaa
     3121 agggaccatt cactgggtat cggcaaagca tgccgtttca ccggaggttc gactttacga
     3181 gcctcttttt aacgtgaagg atcccaatga ttttccggaa ggaaaagata tctttgtcaa
     3241 catgaacccc cagtctctgg aagttttgaa acacgcaaag ctcgaaccgg ctttggggca
     3301 gatggcgccg ggggagcgtg tccagtttga acgcatgggg tatttcttcg tggatccgaa
     3361 agactcccga cccggccagc ccgtttttaa ccggattgtc accctccgtg acacctgggg
     3421 caaaatcgag cagaaacgcc tcgcgtaaaa ttaagtgacg ttgtgcgttg cgtgaagcgg
     3481 ctggactcgc tataattcca cccgttttgc gttcttgccg ggagagaaaa cgggattgcc
     3541 cgtgaggatt ttccctctgc cctgggtttt tggcttacct ccttcattgg ggaatggtgt
     3601 aacggtagca caggtgactc tggatcacct agtctaggtt caaatcctag ttccccaggc
     3661 ttgtaataga accgcttgct ttgaaagaac gatccgaagt cctaaccgcg acaagcactt
     3721 tccaaacaat ctggatggtt cgcctgttac gttgataatc ttgcgattat atttcttgcc
     3781 cgcgcggatg atctttccga aaatctcaat aaacttacac tggaacgtct tcaccgtcca
     3841 ggcgctggcc ccctcctcct aaaaccgctt tatcgctcac ggaacattat ttttctccag
     3901 aattcacgta tttatccgat acttaatatg gcatcccgag cttgtttggt gatcgcattg
     3961 aaccaggaaa ggaaaaacag ccatgggcaa aaaaattctt gtgatagatg atgaggagtt
     4021 gattacaaaa agtcttgtcc ttgccctcga gaaaaatggc tatgaagtgc tcgttgccaa
     4081 acgatgtgac gacgctttgg cgatggcgga agcgattgat tttgatctta ttatcagcga
     4141 cattcgaata cccggggtcg atggcattga aaccgtccgg cggattctag acctgatggc
     4201 tacacgcaag gcaaagcggg ctcccaggat tttcatgacc gggtatgccg acccattaac
     4261 ggaggaaaac gcccaaacat tgaaacccag tgcgtatatt ctgaaaccat tcgatctttt
     4321 agagttttta acagaagtta aaaaagcgct cggggaatag cgtatgccaa acattgatat
     4381 actgcttaaa aatcaggaaa tctcgctttt atccaagata aaaaagtatc gcaaactggc
     4441 caaggaaatt aaaaatcatt tagatcgaga cccagatagc tgggggcagt atcagagtgt
     4501 ttttaatcaa gagataaacg ctatttttcg cgatctcatg ctttttgaaa aggagcggtt
     4561 ggcagaaggc gatgacgtta gcgtttataa attaaaaaaa atgtttatta aaaaattgcg
     4621 cgccgatttt ttgtatggtg aacatattcg atggagcttg gaaaagcctt ttggctacgc
     4681 gggagactat aaaatcattg atgatatgta ccgtaatgat cctcaaacaa acggatttga
     4741 gcgtcttttc gataactact cccaaatgtc tcccgcggcc aacgccattc gcaaccggaa
     4801 acaagatata aagaagttta tttctactgt tttcaaatct cgaaaggggg actgcctaaa
     4861 ggtgatggac ctggcttctg gcccctgtcg agatattaag gaacttttgg atgagctttc
     4921 acctgccgct ggacagattt ctattgattg tttcgatcag gaccagggag ccattgacta
     4981 cgccaaaaac ctgttgcgcg actacccaga aatagtaact ttttatcgac aaaatgccgt
     5041 tcgttttgcc ttaaaaaaag acgtgcctca agggaagtat gatttgattt tttccttggg
     5101 tctgtttgat tatctggatg agcgcatagc tcgtcatttg atcgcggcat taaaaaagca
     5161 actgaaaaaa aatggacttt tgttcattgc gaattttggg gaacaatacc aaaacccgtc
     5221 attttacttt ttggagtggg ttggcgagtg gcagttaatt tatcggagta ttgaagctct
     5281 caaaaagata tttttaatgg caggattttc cgataaaggc cttagaagtg aatcggaaca
     5341 gcagggaatt attcattacg ttattgctaa aaaccccggt taaataaggc atggtcattt
     5401 ttagtattgt cagcagttta accaatgccg cttccagctt gatcttcggt gtttttgtta
     5461 ttcgtcaaaa cagtcg
//
LOCUS       DAOSIO010000116         5466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_13390_length_5466_cov_4.568656, whole genome shotgun sequence.
ACCESSION   DAOSIO010000116 DAOSIO010000000
VERSION     DAOSIO010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13390_length_5466_cov_4.568656"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PK590_05215"
     CDS             <1..779
                     /locus_tag="PK590_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1846 family protein"
                     /protein_id="HOG24029.1"
                     /translation="QTTVNYNRDVEVFPVLKRILKKITGGESYYESPTDMGVNRVGFG
                     IVNDEIVRKAAVQEVIARYFQCACDYRMGYVEKEAVEKTGLLMEDLEVKPEHRPVVEP
                     ARQAAKEAQKKGKGNQGIYCGAALELSDGTIVTGKNTPLMHAASSAVLNAVKAIAGLP
                     EDIHILSPNVIQSIGELKQDVFGSKAVSLDLEEILIALSMSATTNPTAQKAMESLTAL
                     RDQPMHITHIPTPGDEKALRKLRIHFTSDPEFATQNLYDG"
     gene            1044..>5466
                     /locus_tag="PK590_05220"
     CDS             1044..>5466
                     /locus_tag="PK590_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24030.1"
                     /translation="MRKLSQYKGKKGTAIKSIAMSVAVLFLFTDILFFPSQALGQIPT
                     SPIVQGGESLAGFQSQPLYEDGWLPPELGSINEVYRAPGNGEQTAGSGKDESDNASGQ
                     RSSLTIPQSDRMMVFIQDAHDSLEGQENIAKIIRLLTERYGVATVFQEGYEGPVPTDD
                     LFGFIEDPVLKEKISYFFLDKLRLRGAEYAHINRQSGLTHGRQSTDLSQVAHSISGPR
                     TTDRGLDWTLIGADSLKLHRENIGQYRLSAEKKETIERDLGTIEKELKLLVTRRFPKR
                     FREWMKVKEQFDAKKLDLFTYLERTLPLLSHVERQAMDEKQADPNANHLPPTVDAATP
                     LLQFILEAVSSNSAAVLEKAKHIDAREVFAELLKLERMIAETCLADKGRTRHSGARSG
                     MADLNADLQLYHHYQTIQLLKRLNNLEVTQEEYAALLVRHPSSGVRRDELLTRAPHHL
                     EVSTQRLAEFIHSQTKKPLVLSKQWERNIQDAVKFYEIALQRDDALSAALGEYFQDDQ
                     RAQHSQWSGVAPAILVYGGFHKENIKRILEAKGISYVVVSPRITRSSPRHEAYYKQLM
                     TEGFHKFELPFLLHTAASQPTLLNLDPRVARAEIRAVYEAAIRYPDYLETWDRYLNST
                     GPGNIASEMSPGGENLQEVDRRSEVRGEADGKKRKSNSQMSWRSFLKMGLLFFATGWF
                     SMAHTGIAQPSVKRAIFQQPSSVEWTIFRQGNWTVLGARDTGPASAKIRTSAGSPSEI
                     KIVNGRHQIFSVKGNGFLRGTLPGMDWGTSFVLPGYWSQGRYFHNPRITRASFDLNEK
                     GELRLKGAFEDQNGNFVADDFEIVFSMAPRGEATMKVSFTVKAKKDFTLDPERLRNHD
                     GFRIAQFSSMNVGTEHDADMSEYEDKTGERHEIKLENKNQFIFHNPRPFGSERTFYLK
                     NKNPVHGRNNPTTSIRLAESMDPSQFTPQGWITKSKDVNDDNIGGWIHFDAAETGYRR
                     GETIGRFEFVLGIAPNRSETRTQAGKMDEGDSALTVNRIQWQETTQPPTLAETHSGMK
                     AVTPKNTSRDRHGYFLNQLFRALFFQKVLGLDFSQGEHRIVDVGVGALPVTTVEMAER
                     FQEEIPGAISTGRIEMIGIEIPEAVPYAHITAEGLVEKFGFQCSVRPVEEFFRRWDLK
                     AITLTVNEKKEVTGAFVSFDTENGTHYRDIAVRQGTFYLNLGRSYDGWQGGLLDEMIE
                     FLGPEKIRYALSQGVTGTFDGISVELDPVRNMLEKAGVRLVVTDDPWSAGVRDVRLMT
                     VFNVIQHIPQGERLPFLEKLAQSLKKGKADEDGKLIEMGGILAVKGGRPDGTPSFSEV
                     YYTNVNGKLFEIGATRLDFNHAVMSGQKLEGFLVDLEARKLEPVWIPADGADTSRSEI
                     RAQQESRDKGEFITSLANASLQLARHLHDNKFHAVLASGDSAEIPLRLVTTAWQTLYP
                     DESFPLQFVFDGQENNRLYRDWAAVRAFGADRPEDQKR"
BASE COUNT         1414 a         1258 c         1500 g         1294 t
ORIGIN      
        1 agcaaacaac cgtcaattac aatcgcgacg ttgaagtgtt ccccgttctt aaacgaattt
       61 tgaaaaagat tacggggggt gagtcttatt acgaatcccc gacggatatg ggcgtcaatc
      121 gcgtgggatt tggaattgtg aatgatgaga ttgtgagaaa agccgccgtg caagaggtga
      181 tcgcgcgata tttccaatgc gcttgtgatt accggatggg ttatgtggaa aaagaggccg
      241 tcgagaaaac cggtctttta atggaggatc tcgaggtgaa acccgaacac cggcccgtgg
      301 tggagccggc gcgccaggcg gccaaggagg cacagaaaaa agggaaagga aatcagggga
      361 tttactgcgg cgcggcgctt gaattgtccg acggaacgat tgtgaccgga aagaatactc
      421 cgctgatgca cgcggcttcg agcgcggtgt tgaatgccgt caaggccatt gccggtctgc
      481 cggaagatat ccatattttg tcgcccaatg ttatccagtc gatcggagag cttaagcagg
      541 atgtgtttgg gagtaaagcc gtcagtcttg atctggagga aattcttatc gccctgagta
      601 tgagtgccac cacgaatccg accgcgcaaa aagccatgga gagtttgacg gctttgcggg
      661 accagccgat gcacattacc catattccaa cgccaggaga tgagaaagcc cttcgaaagc
      721 tccggattca ctttacgagc gatcctgaat tcgctaccca aaacctttac gacggataaa
      781 aaaccgctca ttttccattc ctttttcggc gggtacttga tcggcagggt ggattagccg
      841 gctaattctt ggttgccaat cagaatgtag taaatttgtc gattctctac agattttaaa
      901 agtggaaacg tatagcaatt tatagcattc tgattgttgt cccgcctttt ggaaccccac
      961 gatttgacta gacattttaa agtgttaacc tttggggcga agtacattta aaaagaattt
     1021 actattgtca aggggttggc taaatgcgga aactatctca gtacaagggt aaaaaaggga
     1081 cggcaatcaa gagcatcgca atgtccgttg cggtgctctt cctttttact gatatcctct
     1141 ttttcccttc acaggccctc ggccaaatac ccacatcacc catagttcag ggaggggaga
     1201 gccttgccgg atttcagagc caaccccttt atgaagacgg ctggctccct cccgaactgg
     1261 gttctatcaa cgaagtttat cgggccccgg ggaacggaga acagacggca ggttctggta
     1321 aagacgaatc ggataacgcg agcggtcagc gttcatcatt aactattcct caaagtgacc
     1381 gtatgatggt gtttatccag gatgctcacg attcacttga agggcaggaa aatatcgcga
     1441 agatcattcg cctgcttacg gaacgttacg gagtcgcgac tgtatttcag gaaggatatg
     1501 aggggccagt cccgacggat gacctttttg gattcataga agatccggtc ctgaaagaaa
     1561 aaatttccta cttttttctg gataagcttc ggttacgggg cgcggagtac gcgcacatta
     1621 atcggcaaag cggattaacc cacgggcgac agtccacgga cctcagccaa gtcgcgcact
     1681 caatcagtgg gccacgaacc acggatcggg gactggactg gacgctcatc ggtgccgaca
     1741 gcctcaagct tcatcgggaa aacatcggtc agtaccggct atccgcggag aaaaaagaaa
     1801 ccattgaacg ggatctcggc acgattgaga aagaattaaa acttctcgtc acccgccggt
     1861 ttccgaagag attcagggaa tggatgaagg tcaaggaaca gttcgacgcg aagaaactgg
     1921 atctgttcac gtatctggaa cgaacgcttc ccctcttgtc acacgttgaa cgtcaggcga
     1981 tggacgaaaa acaggcggac cctaacgcta atcacttacc gcctaccgtt gacgcggcta
     2041 cgcctttact tcagtttatt ttggaggctg tcagttcaaa tagcgccgct gttttggaga
     2101 aggccaagca tattgatgcc cgagaggtgt ttgctgagct cctaaagctg gagcggatga
     2161 tagcggaaac ttgtctggca gataagggca gaacccgtca ctctggggcg cggagcggga
     2221 tggcggatct gaacgctgat cttcaacttt atcatcatta ccaaaccatt cagcttttaa
     2281 aacgcctcaa caatcttgaa gtgacacaag aggagtacgc ggcgcttttg gtacgacatc
     2341 catcgtccgg cgtccggcgg gacgagctgt tgacgagggc cccgcaccat ctcgaggtaa
     2401 gtacccagcg cctcgcggaa ttcatccatt cgcaaacaaa aaaaccgctc gtcttgtcaa
     2461 aacaatggga gcggaatatt caggacgcgg tcaaatttta cgagattgcc cttcagcgag
     2521 atgatgcatt atcagccgcg ctcggcgaat attttcaaga tgaccagcgc gctcaacatt
     2581 cacaatggtc cggcgtggcg ccggcgattt tggtgtatgg cggattccat aaggagaaca
     2641 tcaaacgcat ccttgaggca aaaggcatct cctatgtcgt tgtaagtccc aggattaccc
     2701 gatccagccc tcgccatgaa gcgtattaca aacagctgat gacggaaggg tttcataaat
     2761 tcgaacttcc tttcctgtta cacactgccg ccagccagcc gactctcctg aatttggatc
     2821 cccgggtcgc tcgcgccgaa atccgcgctg tttatgaggc cgcgatacgt tatcccgatt
     2881 atctcgaaac gtgggatcgg tatttaaatt caacgggtcc cggaaacatt gccagcgaga
     2941 tgagcccggg gggcgaaaac cttcaagagg tcgatcgacg gtccgaagtt cggggcgaag
     3001 ctgatggtaa aaaacgaaaa tcaaattctc aaatgagctg gcgaagtttt ctcaagatgg
     3061 ggctactgtt tttcgcaaca ggttggttct caatggctca cacagggatc gcccagcctt
     3121 cggtaaagag agcgattttt cagcaaccct cctcggtgga gtggacgatt tttcgtcaag
     3181 gtaactggac tgtgcttggc gcgcgggata ccggcccagc ttcggcgaag atccgcactt
     3241 cagccgggtc gccttctgaa attaaaattg tgaatggccg gcaccagatt ttttcggtca
     3301 aaggcaatgg attcctgaga ggaacgctcc ccgggatgga ttggggtaca agttttgtcc
     3361 tgccgggcta ctggagccag ggacgttatt tccataatcc caggattact cgcgccagtt
     3421 ttgatcttaa tgaaaaaggt gaacttcgcc tgaaaggggc ttttgaagat caaaatggaa
     3481 attttgtagc ggatgatttt gaaattgttt tttcaatggc tccccggggg gaagctacca
     3541 tgaaggtttc gttcactgtg aaggctaaaa aagattttac cctggacccg gagcggctca
     3601 ggaatcatga tggattcagg atcgcgcagt ttagctccat gaatgtcgga accgagcacg
     3661 acgcggatat gtctgaatat gaagacaaga cgggcgagag gcatgaaata aaactggaaa
     3721 ataaaaacca gtttattttc cacaacccgc ggccgttcgg ttcggaaaga accttttatc
     3781 ttaaaaacaa aaaccccgtg cacgggcgca ataatcccac gacttctatc cggctggctg
     3841 agtccatgga tccttcgcaa ttcactccgc aagggtggat cactaaatcg aaggatgtca
     3901 atgacgacaa cattggcggc tggatccatt ttgatgccgc agaaaccggc tatcggagag
     3961 gtgaaacgat tggccgtttc gagtttgttc tgggaattgc tcccaaccgc tcggaaacga
     4021 gaacccaggc tgggaaaatg gatgaggggg attctgcctt gactgtgaac agaatccagt
     4081 ggcaggaaac gacccagcca ccgacccttg cagaaactca ctctggaatg aaggctgtta
     4141 cgcctaaaaa tacaagcagg gatcgtcacg ggtatttttt aaaccaattg ttcagagcct
     4201 tatttttcca aaaagtcctc gggcttgatt tttcgcaagg ggagcacagg attgtggatg
     4261 tcggggttgg tgcgttacct gtgacgaccg ttgagatggc ggaaagattt caggaggaaa
     4321 ttcccggggc gatcagtacc ggaaggatcg aaatgatcgg gatcgagatc cccgaggcgg
     4381 ttccctacgc gcatattacg gcggaagggc tcgtggagaa atttgggttt cagtgtagtg
     4441 taaggcctgt ggaagagttt tttcgaaggt gggatctcaa agctattact ctaacggtca
     4501 atgaaaaaaa agaagtgaca ggcgcgtttg tgagttttga tacggaaaat ggaacccatt
     4561 acagggatat agcagttcgc caagggacct tttatttgaa tttggggcga tcctatgatg
     4621 gatggcaggg aggtctactt gacgagatga tcgaattttt gggtcctgag aaaatccgtt
     4681 acgcactgtc gcagggagtg accgggacct ttgatggtat ttccgtggag ctggatcctg
     4741 tgcgtaacat gcttgaaaag gcgggtgtaa ggcttgtcgt gacagacgat ccctggagcg
     4801 cgggggtacg ggatgttcgg ctgatgaccg tgtttaatgt tatccagcat attccacagg
     4861 gggagagact tccttttctt gaaaaactgg cccaatcact taaaaagggg aaggccgatg
     4921 aggacgggaa gttgattgaa atggggggga tccttgccgt caagggagga cgtccggacg
     4981 gaaccccatc gttttccgag gtttattaca ctaatgtgaa cggcaagttg tttgaaattg
     5041 gagcgactcg actcgatttt aatcacgcgg tgatgtcggg acagaagctg gaaggattcc
     5101 tggttgacct tgaagccaga aaacttgagc cggtttggat tcctgcggac ggagctgata
     5161 cttcacggtc tgaaattcgc gcgcagcaag aatctcggga caagggcgag tttatcacat
     5221 cacttgccaa tgcttctctc caactcgccc ggcatcttca cgataacaaa ttccacgcgg
     5281 tgcttgcgtc cggcgacagc gcggagattc cccttcgact tgtaacgacg gcctggcaaa
     5341 ccctctatcc tgacgagtca tttcctttgc agtttgtttt tgatggacag gaaaataaca
     5401 ggctttatcg cgattgggcg gctgtacgtg cttttggtgc ggacagaccg gaggatcaga
     5461 agaggt
//
LOCUS       DAOSIO010000117         5430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_13497_length_5430_cov_3.770047, whole genome shotgun sequence.
ACCESSION   DAOSIO010000117 DAOSIO010000000
VERSION     DAOSIO010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13497_length_5430_cov_3.770047"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..1672)
                     /locus_tag="PK590_05225"
     CDS             complement(194..1672)
                     /locus_tag="PK590_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24031.1"
                     /translation="MMKLSKSLMSIFALVLVVGTGLVLSGCATCCGEAAPAKPAATKC
                     ALGVQGCDKGDSVLWIEKLMPDQVVVGQPFQYTIRATNLRSCALEDVVITERASEGLT
                     IQSGSEGLNVGYMKPHETREILLTAVANVGGNPATCTRADYKLVICCGTEAISPKLAL
                     VLEAKAQGILCDSIPATVTVSNSGTGLAKNAVVKLALPQGLSTTDGQTSIGINVGDLK
                     GGDSKAYNINLKAEKAGVYSLKANAEASGLTAASNAVSIDVRQPQLDVSVTGPSKIYV
                     TKDANFKVTAKNTGNVESVNTIVTTSVPEGMRFVSASNGGSLSGNAVVWNVGTLSAGK
                     AVSMDATFNATMGGTGVSVATAKGYCCQNEASTPTKVEGVPAILLEAIDVEDPIQVDG
                     TEKFYVTVTNQGSAPDTNIVIKVSFEDEFDYVSSAGPTQAKSASAKSVEFAPLASLAP
                     GQKATWEVVAKAKATGDHRTSIKLTSDMLTRSVDETESTHVY"
     gene            complement(1778..4126)
                     /locus_tag="PK590_05230"
     CDS             complement(1778..4126)
                     /locus_tag="PK590_05230"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOG24032.1"
                     /translation="MFRKLAEFIDRFRWPILLALIVSLLIMGIAVTRLKVDPSIEPLF
                     IKKSEEYKYYQQYREKYGSDQMVAIAMATDDLFTEENFEILKKVTDGISSFDRVERVI
                     SLANAQDIRHKFLGVKTELAFKEVEEGEKSVDQMRDRILANELYVSNIVSKDGKSANI
                     IAYLKTDGDPAANGEFIEELKDYLIDFERPGLKFHMAGAPIEQYEFIRLIHRDQMVFI
                     PIITLILVATTFLIYRSFACMFLSMSIVFTTLIWTLGTISLLGQELNLMTSLLAPVIM
                     IVAVLNATYLINLFFEIRPHQPSLRESVVLTIQQLGLPCLLTHFTATLGFMSLALNNI
                     PAIQSFGLYAALGTIFAYVIEIVLTVVLVPILPYRPQKVDVFDERNFFNRVLIGFLEN
                     LEARWRWLILLVTALIIIFSIQGVMKIKVDTNIVKQMKPTLPLAIATKFIDENLTGVY
                     SLGFVIRRKDGEHMVDPEALKRLDQFKDYLESENGIAKVNSITTLVKKINEARAGDDD
                     EYAIPEKESLLKLYFKGMAQSGDPELWKMISEDFKEVRIEARMRALGTTEGAALEDRI
                     RDYMDRQMSDFFEYKLTGNVVLLGKISRALFHEQTRSFGFAFASILIVIILIFRSVRL
                     GLLAAIPNLFPILAVYGLMGYMGVELSTATAMIASIVLGLVVDASIQFIYRFEKEFQH
                     RYHYLQALHHTYRNIGQSMVLSAIILCLGFGASIFASFRITVHFGLLTSLTIFLALLC
                     SLLVLPVFLVMVKPFGPQRLFKRRLKGGDSFQERKSLHSRVA"
     gene            complement(4238..4459)
                     /locus_tag="PK590_05235"
     CDS             complement(4238..4459)
                     /locus_tag="PK590_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016860291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOG24033.1"
                     /translation="MKILVMVKANSKHPRVEEVSPGEFRVHVKAPPKEGKANEAVLRA
                     LAEHFHVPLSRIAIVAGMSSKRKWINIHE"
     gene            4565..5020
                     /locus_tag="PK590_05240"
     CDS             4565..5020
                     /locus_tag="PK590_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24034.1"
                     /translation="MKIKILFVLAILGAIAAFGIVLNSGKKIQADPKTSSSSYIELSF
                     SLPVGYQFTQSSPYQLSCESENPDIVKLSRHCGENFSPLHPPYQIAFRGNPGETNVKL
                     TGVFYYCHQETQMCFYDTFETSFPVVVKNHAPSVISRTWDIQPKRISEP"
     gene            complement(5039..>5430)
                     /gene="rfbC"
                     /locus_tag="PK590_05245"
     CDS             complement(5039..>5430)
                     /gene="rfbC"
                     /locus_tag="PK590_05245"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOG24035.1"
                     /translation="LTLDSSDEAKARAQAKLIRVVRGSVYDVAVDLRQGSKTYARYFG
                     TVLSGENRKMLYIPKGFAHGFCVLEDETEFFYKCSEFYSPEHERGIIWNDPEISIAWP
                     RLDCDFILSEKDCHYPTLKNFGRMAKS"
BASE COUNT         1438 a         1480 c         1206 g         1306 t
ORIGIN      
        1 acgatgatcc agataacttt cttcagcatt gttttagaac tcttggcgca atccacaatc
       61 gcgataatat cgagcgccaa aacgatcaaa cctaatagca tccccatggc cgcctccttt
      121 ggatctagtt gttaaaaaca aaggcgcccc gtcgaaaaac ggggcgcctt tgtcacgata
      181 ctacaaccct cgattaataa acgtgggttg attctgtctc gtccaccgat ctcgtgagca
      241 tatcgctcgt aagcttgatc gatgtgcgat gatctcccgt cgccttggct ttcgcgacaa
      301 cttcccaagt cgctttttgg cccggagcaa gagaggccag cggagcaaac tcaacgctct
      361 tcgctgatgc gctcttcgcc tgtgtcggac ccgcggaaga cacgtagtcg aactcatcct
      421 caaagctcac cttgatcacg atgttcgtgt caggcgctga gccctggttg gtcacggtca
      481 cataaaactt ctctgtcccg tcaacctgga tgggatcttc cacgtcaatc gcctcaagca
      541 ggatcgcagg gacaccttcg accttcgtcg gggtacttgc ctcattctga cagcaatagc
      601 ccttcgcggt cgcgacgctc acacctgttc cacccatcgt ggcattaaaa gtcgcatcca
      661 tggaaaccgc ttttccggca gacaaagttc cgacgttcca gacaacagca ttgccggaaa
      721 gtgaaccacc gttagaagct gatacaaacc gcattccttc aggaacgctg gtcgtcacga
      781 tcgtattgac cgattccacg ttaccggtgt tcttggccgt caccttgaaa ttggcatcct
      841 tcgtgacgta aatcttgctc ggccccgtaa cgctcacatc gagttgcggc tgtctgacat
      901 caattgaaac tgcgttcgaa gccgccgtaa gtccgctcgc ttccgcattg gccttgagcg
      961 aatacacgcc agccttctcg gccttcaggt tgatgttgta agcctttgaa tcgccacctt
     1021 tcagatctcc gacgttaatc ccgatcgaag tctgtccgtc ggtcgtgcta agtccctgag
     1081 gcagggccaa cttcacaacc gcgttcttag caagacccgt tccagagttg gaaacggtga
     1141 cggtcgccgg aattgagtcg caaagaattc cctgagcctt ggcttcaaga acaagcgcca
     1201 gtttcgggga gatagcttcc gttccacagc agatcacaag tttataatct gccctggtac
     1261 atgttgccgg gttacccccc acattggcaa cagccgttaa aagaatttca cgggtttcat
     1321 ggggtttcat ataaccaaca ttcaacccct cagaaccgga ctgaatcgta agaccttctg
     1381 aagccctctc tgtgatcaca acatcttcca aagcacacga tctcaggtta gttgccctga
     1441 tggtgtactg gaagggttgc ccgacaacaa cctgatctgg catcaatttc tcgatccaga
     1501 gaacgctgtc gcccttgtca cagccctgga caccaagcgc acattttgtg gccgctggtt
     1561 tcgcaggcgc cgcttcgcca cagcaagtcg cgcaaccgga aagaaccaat cccgtgccaa
     1621 cgaccaaaac cagggcgaaa atactcatga gactctttga gagtttcatc attgcctcct
     1681 ttgatagatg gtgttgatag tcccgaagtt tttatctatt gcttcgagta gggcagtatt
     1741 atatatatat cgcttttaaa aaaatgcacc ttctttctta ggcaactctg gaatgaaggg
     1801 attttcgctc ctggaaggaa tcgccccctt ttaatctccg tttgaataga cgctgaggcc
     1861 caaaaggttt caccatcacg agaaaaaccg gaagcacgag tagcgaacaa agaagcgcca
     1921 ggaaaatcgt gagactcgtc aagagcccaa agtgaaccgt aatgcgaaaa ctcgcgaaaa
     1981 tactcgcgcc aaaaccaagg caaagaataa tggccgacag caccatggac tgtccaatgt
     2041 tccggtaagt atgatgaagc gcctgtagat aatgataccg gtgctgaaac tctttttcga
     2101 accggtaaat gaactggatc gaggcatcca ccacaagtcc caacacaatg ctcgcgatca
     2161 tcgcggtcgc ggtcgaaagc tcaactccca tatatcccat caaaccgtac acggccagga
     2221 tgggaaaaag atttggaatg gccgcgagca atccaagccg aaccgaacgg aaaatcagga
     2281 tgatcacgat caggatcgag gcaaacgcaa atccaaagct cctcgtttgc tcatgaaaca
     2341 gggccctgga aatttttccg agcaaaacga catttcccgt aagtttatac tcaaaaaaat
     2401 cgctcatctg ccggtccatg taatcccgga tccggtcttc aagcgccgca ccctcggtgg
     2461 tacccagagc gcgcatccgg gcctcaatcc ggacctcctt aaaatcctcc gaaatcatct
     2521 tccagagttc cgggtcccct gactgcgcca ttcccttaaa ataaagcttg agaagcgact
     2581 ctttctcggg aattgcgtac tcatcatcat ctccagcgcg cgcttcgttg atttttttga
     2641 caagggtcgt aatagagttc accttcgcga ttccgttttc actctcgaga taatccttga
     2701 actgatccag tcgcttcaaa gcctccggat ccaccatgtg ctccccgtcc ttgcggcgga
     2761 tcacgaaacc caacgaataa acacccgtca gattttcatc gataaacttt gtggcgatcg
     2821 ccagcggcaa agtcggtttc atctgtttta cgatattggt gtctaccttg attttcatca
     2881 caccctggat ggaaaagata atgatcagcg ccgtcacaag taaaatgagc catcgccagc
     2941 gcgcttcaag attttcaaga aaaccgatca gaacccggtt gaaaaagttc ctctcgtcaa
     3001 agacatcgac cttttggggc cgatacggca agatgggaac caacaccacc gtcaaaacaa
     3061 tctcgatcac ataggcgaag atagtcccca gcgcggcgta aaggccaaag ctctggattg
     3121 ccgggatatt attcagggca agggacatga acccgagggt cgcggtaaaa tgagtgagaa
     3181 gacaaggaag tcccaactgc tgaatggtga gcacaaccga ttctctcaaa cttggctgat
     3241 ggggacgaat ttcaaaaaag agattgatga gataagtcgc gttcagcacc gcgacgatca
     3301 tgatcacggg agccagaagc gatgtcatca gattgagctc ctgtccgagg agggagatcg
     3361 tcccaagcgt ccagatgagc gtcgtgaaaa caatcgacat ggaaagaaac atgcacgcga
     3421 aacttcggta gatcaaaaag gtcgtggcca caaggatcag ggtaatgatg ggaataaaaa
     3481 ccatctggtc gcgatgaatc agccggataa actcatattg ctcaattggc gccccggcca
     3541 tatggaattt aagccccggc ctctcaaaat caatcagata gtccttcaac tcttcaataa
     3601 attctccgtt cgcggcagga tcgccatcgg ttttaaggta cgcgataatg ttggcgctct
     3661 tcccatcttt tgaaacgatg ttgctgacat aaagttcatt ggccagaatc cggtctctca
     3721 tctgatcgac gcttttctcc ccctcttcca cttccttgaa ggccaactct gtcttgaccc
     3781 ccaaaaactt gtgccggata tcctgggcat tagcgaggct aatgacccgc tccacgcgat
     3841 caaaactcga aatcccatcc gtcacctttt tcagaatctc aaaattttct tccgtaaaaa
     3901 gatcatctgt cgccatggcg atcgcgacca tctggtcgga accgtatttt tcgcggtatt
     3961 gctggtagta tttgtactct tcggattttt tgatgaagag aggctcgatg gagggatcaa
     4021 cctttaggcg ggtgaccgcg attcccatga taaggagact cacgatcagg gcgagcaata
     4081 tgggccagcg aaaacgatct atgaattcgg ctaattttcg aaacatgaag ttattctatc
     4141 acaacgatta acgcgtcact cacggcgttt ttagaaatcc ttttcagaaa tccatcccca
     4201 tatcagaaaa ggcaagcatg acttcataaa acttttgtca ttcatggata ttgatccatt
     4261 tgcgtttcga agacatgccg gcgactatcg cgatccgact gaggggaaca tgaaaatgct
     4321 ccgccaaagc cctcagcacc gcctcgtttg ctttcccctc tttcggcgga gccttgacgt
     4381 ggactcgaaa ttcaccgggc gaaacttctt caacccgcgg atgtttcgag ttcgctttaa
     4441 ccatcaccaa aattttcatc gccgctcctt tgacacgtga aagcaggaat cagttcggag
     4501 aaaaattcac ttaagacccc gactttttct atgctatgat cccttgagaa aggggaggtt
     4561 tccggtgaaa atcaaaattc ttttcgtgct cgccattttg ggtgccatcg ccgcttttgg
     4621 aatagtcctt aattccggca agaaaatcca ggcggacccc aaaacatctt cttcttctta
     4681 catcgagctt tctttcagct tacccgtggg ataccaattc acccagagtt ctccctacca
     4741 actcagttgc gaaagcgaaa atcccgacat cgtaaaacta agtcggcact gtggagaaaa
     4801 ttttagccca ctccacccac cttaccaaat cgccttccgg ggaaaccctg gagaaaccaa
     4861 cgttaagctg accggagttt tttattattg ccaccaggaa acccaaatgt gtttttatga
     4921 tacctttgaa acaagtttcc ccgtcgtggt aaagaaccac gccccctccg tcatttccag
     4981 aacatgggac atccaaccta aaaggatttc ggagccatag ccgcgccacg tttaattttt
     5041 agcttttagc catccgacca aagtttttca acgtgggata atgacaatct ttttcagaga
     5101 gaatgaagtc gcagtccagc cgcggccacg cgatagaaat ctctggatca ttccagataa
     5161 tcccccgctc atgttcgggc gaataaaact cagagcattt atagaaaaac tccgtctcat
     5221 cctcaagaac acaaaaacca tgcgcaaatc cttttgggat ataaagcatt ttccggttct
     5281 ctcccgacag gacggttcca aaatatcgcg cgtaggtttt tgaaccttgt cgaagatcca
     5341 cggctacgtc ataaaccgaa cctcggacaa cccgaatgag cttggcctgg gccctcgcct
     5401 tggcctcatc ggaggagtcc agcgtcagga
//
LOCUS       DAOSIO010000118         5420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_13524_length_5420_cov_4.027400, whole genome shotgun sequence.
ACCESSION   DAOSIO010000118 DAOSIO010000000
VERSION     DAOSIO010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13524_length_5420_cov_4.027400"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..2258
                     /locus_tag="PK590_05250"
     CDS             297..2258
                     /locus_tag="PK590_05250"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG24036.1"
                     /translation="MTYKKLHFYIIKPSKYDDDGYVIRYWKGVLPSNTLACLYALTKD
                     VRDREVLGKGLQWKITLIDDTVQRPPYEKIFRSAKAKGTKTIVCLAGVQSNQFPRAAD
                     IAFKLRERGIDVLMGGFHVSGVIATLPDLSPELKELRDAGVTLVAGEGEGGHWETSLR
                     DAMENRLRPVYNFLNDVPDLSDAPIPEVLPGTMRRYALSHYGTLDCGRGCPFGCSFCT
                     VINVQGRKMRYRNVDKILKKIRENFHKHGITNYFFTDDNFCRNKNWESIMDGLIEMRE
                     KENMRIHYLMQIDTQSHKVPRFVEKAARSGCQSVFIGMESLNEANLVAVQKTQNKTGE
                     FRELVDSFHRVGIVTHLAYIIGFPFDTAESVKKDVERLISLGSGQTSFFMMTPLPGAM
                     DYKVALEKKMLWDSDLNNYDTFHETHRHARMKPGEWLRAYEKAWHQFYSIENMKHILK
                     NIGREPYWGVFANFVWYKNGFDVEGGHPMIHGFFRLKGRDERRRIFPREKRLDYLRRR
                     VSDIRSVLKGWWKLCWELCEVWISTHERCKNDRGIAAVLEDTPQVHWRDFSLEQIRDL
                     YRQTGLAAPSTFRLIFLKANLFWDNYAPTRAPFAAFWKDTTIKWRRNQFFKINFLRLI
                     SVLIQDSAIFIRFLLAVITCDVRSEMKEE"
     gene            2262..2906
                     /locus_tag="PK590_05255"
     CDS             2262..2906
                     /locus_tag="PK590_05255"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOG24037.1"
                     /translation="MIELIDPRVWPGVIALLALLLMARIFRSRFFSILIFFILFFHLS
                     FFRDLPPRIPEGDSLLSPASGKVVLVDTVAENRFLKEEAWRIRIFLALWNVHVTRSPM
                     EGEVAYQEHVPGKHINVLLPESADKNESNWIGIKDGARSALVRQITGAIARRIYSDVE
                     IGSVVTRGGKLGIICYGSTVEIFVPKRLFEPLIRKGEGVRTGETVIGKWIGEQN"
     gene            2983..3741
                     /gene="pssA"
                     /locus_tag="PK590_05260"
     CDS             2983..3741
                     /gene="pssA"
                     /locus_tag="PK590_05260"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOG24038.1"
                     /translation="MKNRFYFSMAAFPNMITMLNLIAGFFSILLATYGKYEFSVWLIF
                     LALLLDSLDGHVARIFGNATDFGRELDSLADLVSFVVAPCFLMASIFSAMMSVWLLLA
                     IVCYLSAGAFRLARYNVHPTCRGFFEGLPTPAAALTVAMTLLAFRKNGWETAASLVTS
                     AVFLMTAISFLMVSEIPYPKISAVKFRVWRPLFFFQLFVAVFFFFFFNIETSISMVFL
                     AFLVIAPFYCIAKNGISVSEEGSGDNEECSSPSS"
     gene            4050..5108
                     /locus_tag="PK590_05265"
     CDS             4050..5108
                     /locus_tag="PK590_05265"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HOG24039.1"
                     /translation="MALSPRKKNVSKKGIVPKSPASRVPQKKNPISRQRSIKTVKTVK
                     TVKTIKKRPVVEKKAKHVFPTETVKSVPPRQIPAPIPPAIQMELPENYGDNQIYLLIR
                     DPYWLYSYWEIQKSHQEETLKKLGGSWDGVVSILRVFDVTEGSNHPSYFDIVIQNMCR
                     SWYISVQPNHAYVVEIGLLHRDGRYAVLARSNTVRTPRAGMSEVIDEQWMAVDWDKMY
                     ALSGGFQVGKSSAELRELMEERLKGAISSGSGAGLVSSMSSPVKIKKRGFFFWLECEL
                     IIYGGTEPDAEVTMQGRPVSLRPDGTFTLRYALPDGKFTFDCRAKSSDGVEEREITPI
                     IGRTTERPAPIIKKGAKK"
     gene            5112..>5420
                     /locus_tag="PK590_05270"
     CDS             5112..>5420
                     /locus_tag="PK590_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOG24040.1"
                     /translation="MEKGFLSIVLHAHLPFVRHPEHEYFLEETWLYEAISETYIPLIH
                     MMEGLERDRVPWRLTMSLTPSLMSMLRDPLLQDRYIRHVDKLIDLANKEIERTRFMPQ
                     F"
BASE COUNT         1261 a         1190 c         1428 g         1541 t
ORIGIN      
        1 agataggaga taatgtttcc cgtgtgacga aaatatggat gcagaggact gctgaagttg
       61 ccgggttaac cgcgatgatc gagcgatttg ccgcagagtt acacgcgatt tcaacggctg
      121 cgtaatcttt tttcagaaag gataaatcac aaaaaagccg ggttctttgg aacccggctt
      181 tttctttcgc ggtcacatga cccaaatcat tgttccctga gggctatgtt cgcgaaaatt
      241 attagtcgag tgattttttt tgatttatcc agaattgacg atataatcgc cccttcatga
      301 cttataaaaa actccacttc tatatcatca aaccttccaa atatgacgat gacgggtatg
      361 tcatccgtta ctggaagggt gtgctcccct ctaatacact tgcctgtctc tatgccctta
      421 ccaaggatgt gagggatcgg gaggttctcg ggaaaggtct ccagtggaaa attacgctga
      481 ttgatgacac ggtccagcgg ccgccttacg agaagatttt tcgctccgcg aaagcaaagg
      541 ggacaaagac gatagtgtgt ctggctggcg ttcagtccaa tcaatttcct cgcgcggctg
      601 atattgcctt taaactgaga gaacgtggca tcgacgtgct catgggtggt tttcatgtga
      661 gcggagttat cgcgaccttg ccggatcttt ccccggaact gaaggagttg cgggacgcgg
      721 gggtgactct ggttgccggt gaaggggagg gtggccactg ggaaacgtcg cttcgtgacg
      781 cgatggaaaa caggcttcgt cccgtctaca attttttaaa tgatgttcct gatctttcgg
      841 acgcgccgat tcccgaagtt cttcccggaa cgatgcgacg ctacgcgtta agccattacg
      901 ggaccctgga ttgcggccgc gggtgtccgt ttggttgcag tttttgtacg gtcattaacg
      961 tccaggggcg gaagatgcgg tatcgcaatg ttgacaagat tctcaaaaag atccgcgaaa
     1021 attttcataa gcatggtatc accaattact tttttacgga tgacaacttc tgccgcaata
     1081 aaaactggga atcgatcatg gacggattga tcgaaatgcg ggaaaaagaa aacatgcgta
     1141 ttcattatct aatgcagatt gacacgcaat cccacaaggt gccgaggttc gtcgagaagg
     1201 cggcgcgctc cggatgtcag tcggttttta tcgggatgga aagtttgaat gaggcgaatc
     1261 tcgtggctgt tcaaaagacc cagaataaaa ccggagaatt ccgtgaactg gtggattctt
     1321 ttcaccgggt tggtattgtg acccatctcg cttatatcat tggattccct tttgataccg
     1381 ctgaatcggt gaagaaggat gttgagcgtc ttatttcgct tggttcggga caaacctctt
     1441 tttttatgat gacccctttg ccaggcgcga tggattataa agtcgcgctt gaaaagaaga
     1501 tgttatggga ctcggatttg aataattatg acacgttcca tgaaacgcac cggcacgcgc
     1561 gtatgaagcc cggagaatgg ctgagggcgt acgagaaggc ctggcaccag ttctattcca
     1621 ttgaaaacat gaagcacata ttgaaaaata taggccgtga gccctattgg ggagttttcg
     1681 cgaattttgt atggtataaa aatgggtttg atgtggaggg cggacatccc atgattcatg
     1741 gttttttccg actcaaggga agggatgagc ggcgtcgcat tttccctcgc gaaaaaaggt
     1801 tggactacct tcgccgccgc gttagcgaca tccggtccgt gctcaagggg tggtggaaac
     1861 tttgttggga actttgtgag gtctggattt cgactcatga gcgttgtaaa aatgatagag
     1921 ggattgcggc cgtgctcgag gacactcctc aggttcactg gcgtgatttt tcgttggagc
     1981 aaattcggga tctttaccga cagaccgggc ttgcggctcc ctccactttt cggcttattt
     2041 ttttgaaggc caacttgttt tgggataatt atgcgccgac ccgcgcgccg ttcgcggcgt
     2101 tctggaaaga cacaacgatt aagtggcgcc gaaatcagtt ttttaaaatt aacttcctgc
     2161 gacttatatc ggttctgata caggatagcg cgatttttat ccggtttttg ctggccgtca
     2221 ttacctgcga tgtgcgaagc gaaatgaagg aggagtgatc gatgattgag ctgattgacc
     2281 ccagggtttg gcccggtgtc atcgctctgc ttgccttgct gttgatggcc cgtattttcc
     2341 ggtcacgttt tttttcaata ctgatttttt ttattctttt ttttcacctt tcgttttttc
     2401 gtgatcttcc tcccaggatt cctgaggggg attcgttatt gtctcccgcg agcggtaaag
     2461 tggtgctggt cgacacggtt gccgagaatc ggtttttaaa ggaagaagcc tggcgaatcc
     2521 ggatcttttt ggcgctttgg aatgtgcacg tgacacggtc gccgatggag ggggaggttg
     2581 cttatcagga acacgttccg ggcaaacaca tcaatgtgtt gttgccggaa tccgccgaca
     2641 aaaatgaatc aaactggatc ggaattaaag atggcgcgcg aagcgccctt gtgcgccaga
     2701 ttacgggggc gatcgcgcgc cgaatttact cagatgtcga gatcggaagt gtcgtgaccc
     2761 gcggtggaaa attgggcatt atctgttatg gttcaacagt cgagattttt gtcccgaagc
     2821 ggttgtttga gcccttgatc cgaaaaggag agggtgttag aacgggtgag acggtgatcg
     2881 ggaagtggat cggggaacaa aactgaaacc ggcagatgtt gccttaaagg agttaaggga
     2941 atggtgattg gttttgagaa actcaggtgg tgacataaaa caatgaagaa tcgtttttat
     3001 ttttcgatgg cggctttccc caacatgatt accatgttga atctcatcgc gggatttttt
     3061 tcaatcctcc tcgccacgta cggaaaatac gaattttcag tctggctcat ctttctcgcg
     3121 cttttactcg attcactcga cgggcatgtg gcgcgcatat tcggaaacgc gacggatttt
     3181 gggagggaac tagattctct cgccgatctt gtgtcctttg tcgtggcccc gtgttttttg
     3241 atggcgtcta ttttttcagc catgatgtcg gtttggcttt tgctcgcgat cgtctgttat
     3301 ctttccgcgg gggcttttcg tctggctcgt tacaatgtcc atccgacgtg tcgtggtttc
     3361 tttgaaggtt tgccgactcc cgccgcggct cttacggtgg ccatgacgtt gctcgcgttt
     3421 cggaaaaacg gatgggagac cgcggcatcg cttgttacga gcgctgtttt tctgatgacc
     3481 gcgatcagtt ttttgatggt aagtgaaatt ccttacccta aaatctcggc ggtgaaattc
     3541 cgggtttgga ggccgctttt ctttttccag ctttttgtcg cggtgttctt ttttttcttt
     3601 ttcaatatcg aaacttccat ttcaatggtt tttctcgcgt ttctggtcat tgccccattt
     3661 tactgtatcg cgaagaacgg aatatccgtt tctgaggaag gttcaggcga taatgaggag
     3721 tgctcgtccc cctcatcctg aagttgccat ttgttccgta gggatgaaaa tgggttcttt
     3781 tactcgtgaa ggtaaagcct ccgggagtgg gtcctgaaat ccgaaaatca tctcaaaatt
     3841 ccttcaaaat tccttcaaaa atcttttatt gcaggaggtc ggcaactttg ctaaaatccc
     3901 gtcgcctacc ttataacttc gtggttgaag gtgacagagt ttttggggtt ttattttctt
     3961 acttttgtga taagtttgaa gttctgtctt gatcgttgat gttttttttc gctagttctt
     4021 tttaatagcc agtaaaaagg gtttaaacca tggccttgtc accacgaaaa aaaaatgttt
     4081 ccaaaaaagg gattgttccc aagagccctg cttcccgtgt tccccagaaa aaaaatccga
     4141 tctcccgtca acgatccatt aagacagtca aaacagttaa aacggtcaaa acaataaaga
     4201 aacggccagt ggtggaaaag aaagcaaagc atgtctttcc cactgagacg gtaaagtcag
     4261 taccgccaag gcagataccc gcaccgatcc ctcctgcgat ccagatggag cttccggaaa
     4321 attatggtga caatcagatt tatcttttga ttcgtgatcc gtactggttg tattcgtact
     4381 gggaaatcca gaagtctcat caggaggaga cactcaaaaa gttagggggc tcatgggatg
     4441 gtgtcgtgtc gatcctccgg gtatttgatg ttaccgaggg ttcgaatcat ccctcttatt
     4501 ttgacattgt gatccagaac atgtgtcgca gctggtatat cagtgtccag cccaatcatg
     4561 cttatgtggt ggagatcggt cttttgcatc gtgatgggcg ttatgccgtt cttgcccgtt
     4621 ccaatacggt tcggaccccg cgcgccggaa tgtctgaagt cattgacgaa caatggatgg
     4681 ccgttgactg ggacaagatg tacgcccttt cgggcgggtt tcaggttgga aagagctccg
     4741 ctgagcttcg tgagcttatg gaagaacggt tgaaaggggc catcagctcc ggttctggcg
     4801 ccggacttgt ctccagtatg tcaagtccgg tgaaaattaa aaagagaggt tttttcttct
     4861 ggcttgaatg tgagcttatt atttatggag gtacggagcc ggacgcggag gtcaccatgc
     4921 aggggaggcc ggtttcgctt cgaccggatg gaacctttac tttgcggtac gcgcttccag
     4981 acggaaagtt tacttttgat tgccgcgcaa aatcatcgga tggtgttgaa gaacgtgaaa
     5041 tcacgccaat catcggtcgc accacggaaa gacccgcgcc gattatcaaa aaaggcgcta
     5101 agaaataatc catggaaaaa ggttttcttt cgatcgttct ccacgcgcac cttcctttcg
     5161 tccgccatcc ggaacatgaa tactttctgg aagaaacatg gctctatgaa gccatttcag
     5221 agacttatat tccgctgatt cacatgatgg aagggcttga gcgcgaccgg gttccgtggc
     5281 ggttgaccat gtcactgacg ccatcgctga tgtccatgct tcgtgacccc ctccttcagg
     5341 atcgctacat ccgacacgtc gacaagctga tcgatctggc taacaaagaa attgaacgca
     5401 cgcggtttat gcctcagttt
//
LOCUS       DAOSIO010000119         5414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_13547_length_5414_cov_3.481247, whole genome shotgun sequence.
ACCESSION   DAOSIO010000119 DAOSIO010000000
VERSION     DAOSIO010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13547_length_5414_cov_3.481247"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PK590_05275"
     CDS             <1..216
                     /locus_tag="PK590_05275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG24041.1"
                     /translation="IADSGLGMPEEVRKQVFVPFFTTKATAIKGTGLGLYVIRKMVDA
                     HQGEIELESVYGEGTTFRIFLPATGET"
     gene            218..577
                     /locus_tag="PK590_05280"
     CDS             218..577
                     /locus_tag="PK590_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG24042.1"
                     /translation="MAKIVIVDDEEDLLEFMESYFKSRGHEVFTAVGGREAIPLILNQ
                     RPDVALIDVRLKDDVDGLKVIRDVRGKAPDQKMILMTAYNDKDEEGIACGATLCIHKP
                     TSLNEIETAVLNVINAQ"
     gene            642..1463
                     /locus_tag="PK590_05285"
     CDS             642..1463
                     /locus_tag="PK590_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG24043.1"
                     /translation="MDIPKSRGTILIVDDEPDTLWFISKTGQALGYSTLTAGSGMEAL
                     KIIRECGARIDLVVLDLSMPGMSGIEVLRLIRQDYPQLGVIVLTAMHDEQKRCEQLGV
                     DAFIRKPYSLRDLHDRIEVMIERKNEDKMPLEIESGWEISAKVLIVDDEEEVCDFLKS
                     ALTENVKDARFEVACALSGDDALRISRKFEPDIGIVDIKMPHMWGDELIERFKAGEGY
                     APKDFIIYTAVDAAEELRRAKQLGYRFFPKPTAVETLMEVIRKICVKHRLLRKKP"
     gene            complement(1617..2255)
                     /locus_tag="PK590_05290"
     CDS             complement(1617..2255)
                     /locus_tag="PK590_05290"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG24044.1"
                     /translation="MYEEIRKDLEDQGKLKRPPRIPHWGKAIKSLLVQKSKSEHSLSV
                     SWLAKTAGINEKNLHNIIAGRVRDPASDKLVRIADAFEISFSELAARAMAETPGNFFI
                     CGYGERAFIDYPQHGFTIQSLSPQSTSQRDFFFGKMTIKPFTQLKRWRFRENSSVAIF
                     LETGTLEITYGDQRRNIHANESVYFDADVPHKIKNIDSIEAKLFIVTCPALF"
     gene            2439..2840
                     /locus_tag="PK590_05295"
     CDS             2439..2840
                     /locus_tag="PK590_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24045.1"
                     /translation="MAIEKQKMGREGKSGNFLSGRQSLEEDGEIRELFTGYFRRKMAL
                     VLLLIRSQDRSFEKRQVEKAVRFLDDLYRHLSRGGGRKKYRRVFRWLTCFFACRRDET
                     LSRGVKAEKLVRLIEDEVVLRYKMTPGDEVW"
     gene            2840..3187
                     /locus_tag="PK590_05300"
     CDS             2840..3187
                     /locus_tag="PK590_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24046.1"
                     /translation="MEWTKKVSFDASHLSFKSDYEPSYQRCLLKAVSRVQNLLTQDET
                     IADDARIHAAKSLLYAAIEQMGTQMEKMGTIQRLLRELLLIANSQYESPGRDRELGLQ
                     ILADIRSKTDFFG"
     gene            3404..>5414
                     /gene="treY"
                     /locus_tag="PK590_05305"
     CDS             3404..>5414
                     /gene="treY"
                     /locus_tag="PK590_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HOG24047.1"
                     /translation="MSLAKDRPSENGRPVYCPSSVYRLQMNAGFTFRDAKSILPFLHD
                     LGVEAVYLSPIFKAVPGSSHGYDVTDPTVLNPELGGQAEFDAFCDKIRELKMGIILDT
                     VPNHMGIAGSHNALWLDVLENGPSSVHAGFFDIDWKPVKAELENKILIPILGDLYGLV
                     LDRREIQLELDDSKGAFYVRYFDNRFPVDPQTYPTILEFRIDELKGHLNEDEAVVSDY
                     LSVITAFKNLPSRTETNIEKIRERMREKEVAKKRFAEILSRSRLIKEFVESRLVIYNG
                     VKNDSASFDLLDRFLDEQVYRMAFWRTAAEEINYRRFFDINELAAIRMEDERVFEHCH
                     RLIFQWIAEGKVHGLRIDHPDGLYDPPVYFRRLQEKFVAHRKECRKISSREIRSSAKP
                     LYVVAEKILDRKEFLPENWSVHGTVGYDFLNVLNGVFVDQNHQKDFSELYETFIGHTI
                     DFDQLLYDKKKLFALVHMASEINTLGHRLDLISEKDRHYRDFTRNNLTLAIREVIACF
                     PVYRTYLSPSDESVSERDERYINIAVEKAKAKTPALNPAVYDFLREVLLLKFDVGVTV
                     EAKKIYRDFVLRFQQLTGPIMAKGMEDTSFYVYNRFVSLNEVGGDPFRFGHSKEEFHQ
                     FNKLKSERWPYGMLCTSTHDAKRSEDVRMRLNVLSEIPDDWRSVLT"
BASE COUNT         1313 a         1166 c         1536 g         1399 t
ORIGIN      
        1 atcgcggatt ccggtctcgg gatgccggag gaagtgagga aacaggtatt tgtccctttt
       61 ttcacgacca aggcgacggc cataaagggg acggggcttg ggctgtacgt gatccggaag
      121 atggtagacg cgcatcaggg agagattgag cttgaatccg tttacgggga gggcacgacc
      181 tttcgcatct ttctgcccgc cacgggggaa acgtagagtg gccaaaattg tcattgtgga
      241 tgatgaggaa gatcttctgg agtttatgga gagttatttt aagtctcggg ggcatgaagt
      301 gttcacggca gtcggtggaa gggaagcgat accgctaatt ctgaatcagc ggccggacgt
      361 ggcgctgatc gacgttcggt taaaagatga tgtggatggt ctcaaagtga tcagagacgt
      421 ccgtggaaag gcgcctgatc agaaaatgat tttgatgacg gcgtataacg acaaggacga
      481 agaggggatt gcgtgcgggg cgacgttatg tattcacaag cccacaagtt taaacgagat
      541 tgaaacggcg gttcttaacg tgatcaacgc tcaatagaat tcgagggccc cggaagggtg
      601 gggctgtagc gttcgaacgg gaaggcttgg ggaggtgcgg gatggatata ccaaagtcgc
      661 gcggaactat tcttatcgtg gatgatgaac cggatacact ctggttcatt tcgaagaccg
      721 gacaggcgct tggctatagc acgctgacgg ccggaagtgg aatggaagct ctcaagatta
      781 tccgtgaatg tggggcccgg attgatcttg tggtccttga cttgagcatg ccggggatga
      841 gcggtatcga ggtcttgcgg ctcatacgcc aggattatcc tcaacttggc gtgattgtcc
      901 tgacggcgat gcatgacgaa caaaaacgat gcgaacagct tggagtcgat gcgttcatca
      961 ggaagccgta tagcctgagg gatttgcacg accggatcga ggtgatgatc gaacgaaaga
     1021 atgaggacaa gatgccgctc gaaattgaaa gcggatggga gatttcggca aaggtgctga
     1081 ttgtggatga tgaggaggag gtttgcgatt tccttaaatc agcattaact gaaaacgtga
     1141 aggacgcgcg ttttgaagta gcttgcgctc tttcggggga tgatgctctt cggatttccc
     1201 ggaagtttga gccggatatc gggatcgttg atatcaagat gccgcacatg tggggggatg
     1261 aactgattga acgtttcaag gccggagagg gatacgcccc gaaagatttt attatttaca
     1321 cggcagtgga cgcggccgag gagcttcgtc gcgccaagca gttgggctat cgcttttttc
     1381 caaagccaac ggccgttgag acgttaatgg aagtcatcag gaaaatttgc gtgaagcaca
     1441 ggttgctaag gaaaaagcct taggcaaaca tgtttgaagc gcccgcacgt tgtcttccgc
     1501 gttacccgta agctcccccc caccggctga tttggatttt tcatcgaaat gacgcgtggt
     1561 tttctcgcga tttgctaccg gatgagtttt tgagtttgac caccggactg acctgatcaa
     1621 aagagggcgg ggcaggtgac gatgaaaagc ttggcttcaa tggaatcgat attcttgatt
     1681 ttatgcggta catcggcatc gaaatagacg gattcgttcg cgtgaatatt ccttcgttga
     1741 tctccgtacg ttatctccag ggttccggtt tccagaaaaa tagccacgct ggaattttcc
     1801 cggaaacgcc aacgcttcag ttgcgtgaac ggtttgattg tcattttgcc aaagaaaaaa
     1861 tcccgctggc tcgtggattg cgggcttaac gactggatcg taaagccatg ctggggataa
     1921 tcgataaacg cgcgttctcc gtacccgcag atgaaaaaat taccgggggt ctcggccatg
     1981 gctcgggcgg ccagttcact gaaggatatt tcgaaagcgt ccgcgattct gacaagcttg
     2041 tcggaggcgg ggtcgcgaac ccgtccggcg atgatgttat gcagattttt ttcgttaatc
     2101 cccgcagttt tggcgagcca gcttaccgag agtgagtgct cagattttga tttttggaca
     2161 agtaacgatt tgatcgcctt gccccagtgg gggattctgg gaggcctttt caacttcccc
     2221 tgatcttcca ggtcttttcg aatctcttcg tacattgaag cccctttttg ttgttttttt
     2281 aatatcctaa tcagttcttt tacctaactc aaagggtagc attatgaata aggttttgca
     2341 aataaaccaa aagcttattt taaaaaactt tttgcctttt ccttgtcttt cgtaacggag
     2401 ggtgttaatc gttccgtcag gagaacacga tgataaccat ggcaatcgaa aagcaaaaga
     2461 tgggtcggga aggaaaatca ggaaattttt tgtcgggacg gcaatccctg gaggaggacg
     2521 gtgaaattcg ggaacttttt accgggtatt tccggaggaa gatggccctt gtgttgctac
     2581 tgatccgctc gcaagaccgg agtttcgaga aacggcaggt ggaaaaggcg gttcgatttc
     2641 ttgatgattt gtaccggcac ctgtcgagag gtgggggccg taaaaaatat cgcagggtat
     2701 tccggtggct gacttgtttt tttgcgtgtc gaagggacga gacattgtct cgcggcgtaa
     2761 aggcggaaaa gctggttcga ctgattgagg acgaagttgt tttgcggtac aaaatgacgc
     2821 cgggggatga ggtgtggtga tggaatggac aaaaaaagtg agttttgatg ccagtcacct
     2881 ttcgttcaaa agcgactatg agccgtccta ccagcgttgt cttctcaagg ccgtgagccg
     2941 cgtacagaac cttctgaccc aggatgaaac catcgcggat gatgcccgga tccacgcggc
     3001 caagtcgctc ctttacgcgg cgatcgaaca gatgggaacc caaatggaaa aaatggggac
     3061 gattcagcgg ttgcttcgcg agcttcttct tattgccaat tcgcagtatg agtctcccgg
     3121 ccgcgatcgg gagcttggcc tgcaaattct tgccgatatc agatccaaga ctgatttctt
     3181 cggataagta agtggcggtc ttccggcact tcgataaagg cgaatttcca tgtcggggag
     3241 cggtcttttc gagtgtggcc cgcgatgtta atgtcatcaa ttggcgcgtt atgacacgtt
     3301 agaaaaattt aaattttcct tcattaaagt ttcatggata taaaatcgtg tgataagatt
     3361 gcggatcaca acacccccgc aagattcaaa aggagagtca tacgtgtcac ttgcaaaaga
     3421 tcgtccgtct gaaaatggcc gaccggtgta ttgtccatcg tcggtgtacc ggcttcagat
     3481 gaatgccgga tttacctttc gtgacgcaaa gtcgattttg ccctttcttc acgatcttgg
     3541 cgtggaggcc gtctacctgt cgccgatttt taaggccgtt ccgggaagtt ctcacggata
     3601 tgacgtgact gatcccacgg tgctgaatcc ggagcttggg ggccaggccg aatttgacgc
     3661 tttttgtgac aaaatccggg agctaaaaat gggaattatc cttgatacgg ttcccaatca
     3721 catgggaatt gccggcagtc acaacgcgct ttggctggac gtccttgaaa acgggccgag
     3781 ttcggtccac gctggttttt ttgatatcga ctggaagccg gtcaaggcgg agcttgaaaa
     3841 taaaattctg attcccatcc tgggggattt gtacggactc gttttggatc ggcgggagat
     3901 acaactggaa ctggatgatt caaaaggagc gttttatgtc cggtattttg acaaccggtt
     3961 tccggtggat cctcagacct atccgaccat ccttgaattc cggatcgacg aacttaaggg
     4021 ccacctgaat gaggatgagg cggtggtgtc ggattattta agcgtgataa cggcgttcaa
     4081 aaaccttccc tcaagaaccg agacgaatat tgaaaaaatc agggaacgaa tgcgtgagaa
     4141 ggaagttgcc aaaaaaaggt ttgctgaaat tttatcgcgg tcgaggctca tcaaggagtt
     4201 tgtcgagagt cggcttgtta tctataacgg cgttaaaaat gattcggcga gttttgatct
     4261 tcttgaccgg tttctggacg agcaggttta ccggatggct ttctggcgga cggcggccga
     4321 ggagatcaat taccgccgtt tttttgacat caatgaactt gccgcgatcc ggatggagga
     4381 tgagcgggtt tttgaacact gtcaccggct tatttttcag tggatcgcgg aagggaaggt
     4441 ccacggcctc cgaattgatc atcctgacgg actttatgat cctccggtct acttccggcg
     4501 gcttcaggag aagtttgtgg cgcaccggaa agaatgccgg aaaatttcga gccgcgaaat
     4561 ccgctcttcc gccaagcccc tttacgtcgt ggcggagaaa attctggatc ggaaagagtt
     4621 tttgcccgaa aactggagcg ttcatggcac ggtgggttat gacttcctta atgtcctcaa
     4681 cggggttttt gtggaccaga atcaccagaa ggatttttcc gagctttatg agacctttat
     4741 cgggcatacg attgattttg accagttgct ttacgataag aaaaagctgt ttgcccttgt
     4801 ccacatggcg agtgaaatca ataccctcgg gcaccggctg gacctgattt cggaaaaaga
     4861 tcgtcattac cgggacttta cgagaaataa cctgaccctc gcgattcgtg aagtgatcgc
     4921 ctgttttccc gtttaccgga cctatctgtc gccttcggat gaatcggttt cggagcgtga
     4981 tgaacgatac atcaatatcg ccgttgaaaa agcgaaagcc aaaacgccgg ccttgaatcc
     5041 cgccgtatac gatttcctga gggaagtcct cttgctgaag tttgatgtcg gagtgacggt
     5101 ggaggcgaaa aaaatctatc gagattttgt gcttcgattc cagcaattga ccggcccgat
     5161 catggctaag gggatggagg acacttcttt ctacgtttat aaccgctttg tttctcttaa
     5221 tgaagttggc ggcgatcctt tccgtttcgg acattccaag gaggaatttc atcagtttaa
     5281 caagcttaag tctgaacgct ggccttacgg gatgctttgt acgtcgactc acgatgccaa
     5341 acgcagtgaa gacgtccgca tgcgcctgaa tgtgctcagt gaaattccgg atgactggcg
     5401 gagcgtatta accc
//
LOCUS       DAOSIO010000120         5390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_13604_length_5390_cov_3.951828, whole genome shotgun sequence.
ACCESSION   DAOSIO010000120 DAOSIO010000000
VERSION     DAOSIO010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13604_length_5390_cov_3.951828"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PK590_05310"
     CDS             complement(<1..232)
                     /locus_tag="PK590_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24048.1"
                     /translation="MLNNRSASFRLILSILIVCLSFQGCITYRPVALEKVPFMERAQT
                     QTQQDVTVTDAVMSDQESKKAFGVPLAKRGLQP"
     gene            335..1123
                     /locus_tag="PK590_05315"
     CDS             335..1123
                     /locus_tag="PK590_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOG24049.1"
                     /translation="MKHFFPWIAVFFLVGCATVPMTGRKQLSFIPQGQLLTLSDQSYQ
                     EILGSVTLSRDAAKTAELVEVGRRVSSAAEAFMRENGMERDIANYRWEFKLVQDDKTV
                     NAFCMPGGKIVFYTGIFPVAQDANGLATVMGHEIAHAIANHGGERMSQQLMVQAGGLG
                     LAQLTQSKPEMARQILMQVYGAGAQVGLLLPYSRAHEFEADRIGLMLMARAGYDPKAA
                     VNFWERMRKLGGARPPEFLSTHPADSRRIADLQKAIPEAEKYYK"
     gene            1323..2771
                     /locus_tag="PK590_05320"
     CDS             1323..2771
                     /locus_tag="PK590_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOG24050.1"
                     /translation="MSGIYGVVSKKDCIETLFYGTDYHSHLGTEFGGLAVMNGDRFFR
                     QIHEIGQSQFKSKFFEDIPELAGNKGIGVISDSNEQPIYLNSKFGPFCIATMGLIENA
                     ESLVRELLDQGHSFSEMSAKGPVNTTELIARLIALGDNLIDGIDRMFDKIEGSCSLLI
                     LNKDGVYAARDRWGYTPLVVAKHAETYAIASETSSFPNSDLKIMKYLAPGEIVLLTED
                     GLVSQKEGNGAMQVCTFLWIYMGFPASDYEGISTEAVRERCGAALARRDKDIKADVVA
                     GVPDSGTSHAIGYAIESGKPFRRPLVKYTPGYGRSYTPPNQEQRDLIARMKLVPIREV
                     INGNRIVLCDDSIVRGTQLKNFTINKLWDSGAKEIHVRPACPPIMFPCKFCLSTRSTH
                     ELVARRAIRNIEGCDVEDVSEYVNSDSSRYKKMVDWITRDLGVTTLRYQTVEDMVKAI
                     GLPREKLCLYCWTGECPNKCQGSKSKCENNSL"
     gene            3192..>5390
                     /locus_tag="PK590_05325"
     CDS             3192..>5390
                     /locus_tag="PK590_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24051.1"
                     /translation="MSKKINQGLSTSHTPFHQGKSLISVVTLVAFITTTIWNPGVSFA
                     MTPLPASEVNLPYQASIDSTFKFFIPKDLGKVESMDFGQGPGIVHIQTAHGHYEAQQK
                     IRKILHHLDKNYGIRTLLVEGSAFKLRPEILNFFPEDPKRTMKVADRLAQQALVKGPE
                     LYLLDRIGTAGAGDRVLRPAEAYGIEDARIYRENGEAFVRVLTQKQKTEQFLRDMDEG
                     ITRLSAPHLSDSLRRFIRTQESFEKRLMSVDAWFSYLRDETRKNLLLDLGHPANQIEW
                     PMMVRLFKIRELQKNLDLQQFGKEREKFLKAVERFIPEEGQAAGNDDLVSAARQVSVR
                     PISFLAQIGELLQIDRMGQQLPDPETSLLFEAMVRRLPSNFNYRLYPNVCYFIGILLL
                     TSEVKADRLMDESSRVAEMITSRLAQTDSEKAIVALLKDYRLLQKLFALELLPEDYET
                     ILASSAIGGSSLSNGQNVLSPSSIVRRLESLASQTPGAGRSGKVQFSHLDELDGLFDS
                     AMQFYAGVKARDLAMIGMVEKRLKETGVGKVAVVTGGFHARPFRDYFSSQGYTYALVT
                     PKITDQDEAGHRHYIENTLRMTRVGAEPASNHQATNQASSHLAFHARTSTRETVPLMH
                     TRQELRAMGVRAGLVEIARMAGVPLTAINRAEVRLTKQVADEVSRQVGSSQRALENIK
                     QAPTFDELNPDVQNAFWEVFQNTRLIPDEAYLDDWYVQNREGVIHALENPP"
BASE COUNT         1346 a         1216 c         1479 g         1349 t
ORIGIN      
        1 cgggttgaag cccacgtttt gccaggggaa cgccaaaggc ttttttactt tcctggtcgc
       61 tcattaccgc gtcggtcacg gtgacgtctt gctgggtttg tgtctgggcc cgctccataa
      121 agggcacctt ttccagggcc acgggacggt aggtgataca tccctggaag ctcaggcaca
      181 cgatcagaat ggagaggatc aagcgaaacg aagcggaacg gttattaagc atatgtctcc
      241 atattccatc tggttttaag ggatagacta cattataata cctgacctta atgtttttcc
      301 aacagcttat tcacccattc tgtggagacg ccctatgaaa catttttttc cctggatagc
      361 agtctttttt ctggtaggtt gcgcgacggt accgatgacc ggacgaaaac agctttcctt
      421 tattccccag ggtcagctat taacattgag tgatcaaagt tatcaggaaa tactggggag
      481 cgtcacactt tcacgggatg ccgccaaaac ggccgaatta gttgaggtcg ggcggagggt
      541 ttcatcggcg gccgaagctt ttatgagaga aaacgggatg gaaagggaca ttgctaatta
      601 ccggtgggaa ttcaaactgg tacaagatga taaaacggtc aatgcctttt gcatgcccgg
      661 cggaaaaatt gttttttata caggcatctt ccccgtggcc caggacgcca atggtctcgc
      721 gacggtgatg ggacatgaga tcgcgcacgc tatcgcgaac cacggtggcg agcgcatgag
      781 ccagcagttg atggtccagg cgggaggact ggggctcgcg caacttaccc aatccaaacc
      841 ggaaatggca cggcagattt taatgcaggt ttacggcgcg ggggcccagg tgggattgct
      901 gttgccttac agccgggcgc atgaatttga ggcggatcga atcggtctga tgcttatggc
      961 ccgggcgggt tatgatccga aggcagcggt taatttttgg gaacgtatga ggaaactcgg
     1021 gggggcacgg cctccggaat ttttatctac ccatcccgcg gacagtcgac gaattgcgga
     1081 tctccaaaag gcaattccgg aagcggagaa gtattacaag taaaaagcgg ggactttgcg
     1141 tgcgcggaga aagttttccg tcccgacttt gccggctcgc ggtttaaagg aaaagggtta
     1201 cggcagaggc cgcctccgga tcaccaaaag tggaaaagct tgaactcaaa atgacggaaa
     1261 ctgccggtat caaaatcaaa cgaaagcgct gacaagtttt ttgcatttcg aagaggtgat
     1321 gtatgagcgg tatttacggg gttgtttcga agaaggattg catcgaaacg cttttttatg
     1381 gtacggatta tcactcccac ctgggaacgg aatttggtgg tctggcggtg atgaatggag
     1441 accgattttt ccggcaaatt cacgagatcg gacaaagtca gtttaagtca aagttttttg
     1501 aggacattcc ggaactggcc ggcaacaagg gcattggcgt tatcagcgat tccaacgaac
     1561 aaccgattta tcttaattcc aaatttggtc cgttttgcat cgcgacgatg gggttgattg
     1621 aaaatgccga atcgcttgtg cgggaacttc ttgaccaagg tcattctttt tcggaaatga
     1681 gcgcgaaggg cccggtgaac acgactgagt tgatcgcgcg gcttattgcg cttggggaca
     1741 atttgatcga cgggattgat cgtatgttcg ataaaatcga agggtcctgc tcgctcctta
     1801 tcctgaataa agacggggtc tacgcggccc gtgaccggtg gggatacaca ccgctggtgg
     1861 tggcaaaaca cgccgagact tacgcgatcg cttcggaaac ttcatcgttt ccgaattcgg
     1921 atcttaaaat catgaaatat ttggcgcctg gcgagatcgt tttgctgacg gaagatgggc
     1981 tggtgtcgca gaaggaaggt aacggcgcga tgcaggtttg cacgtttctc tggatttata
     2041 tggggtttcc ggcctcggat tacgagggga tcagcacgga ggccgtgcga gagcgctgtg
     2101 gggcggcgct cgcgcgaagg gacaaggaca tcaaggcgga tgtagtggcg ggagtgccgg
     2161 atagcgggac aagccacgcg atcggttacg cgatagaaag cgggaagccg tttcgtcgcc
     2221 cgctggtcaa gtatacgcct ggatatggcc ggagctatac acctcccaac caggaacaac
     2281 gggatctgat cgcaaggatg aaactggttc cgatccgtga ggttattaat ggtaaccgaa
     2341 ttgtgctctg tgacgactcg attgtccgtg gaactcagct taagaatttc acgatcaata
     2401 aactgtggga ctcgggcgcg aaagaaatcc acgtcaggcc ggcttgtccg ccgatcatgt
     2461 ttccctgcaa gttctgtctt tcgacgcgga gcactcacga gcttgtcgcc cgcagggcca
     2521 tccgcaatat cgagggatgt gatgtcgagg atgtttctga gtatgtgaat tctgacagtt
     2581 ccaggtacaa gaaaatggtg gattggatca cgcgcgacct cggggtcacg actctccgct
     2641 atcaaacggt tgaggatatg gtgaaagcga ttggtcttcc cagggaaaaa ctttgccttt
     2701 actgctggac cggggagtgt cccaataaat gtcaggggtc caaaagtaag tgcgaaaata
     2761 acagtttgta aatccggcaa agaccatgag acaactttca ccgacgggaa tgaaagttgt
     2821 gaccgcgatt tttggatttc tgccagcgtg gatttataat cagttttttc cccaagggca
     2881 aaggggatcc gaggtttgtt cttgtaccct ctgaattttc gaaaaaataa gcctcatgga
     2941 aggtgatttt gtccccgtga aaaagttatg agatgctata ttttgaaagc gatagagaag
     3001 tgatcgctta acacgataaa agaagtcgtt tgtgtagtag agttttaaaa ctgcaaataa
     3061 ttctccccac ccttgtaatt acaaaaagtg tcagttatat tttccaagac ttttgtaaaa
     3121 atcaaagtaa gtccaggcta ttattttttt tagtcagata actcattcct ccggaagaaa
     3181 ggtgtcgcta aatgtccaag aaaatcaatc aaggattaag taccagtcat acgccttttc
     3241 atcaaggtaa gtcactcata tcggtggtga cgcttgttgc ctttattacg acgacaatct
     3301 ggaatcctgg ggtgtctttt gccatgacgc ctttgccagc gtccgaggtt aaccttccgt
     3361 atcaggccag cattgattcg acttttaaat tttttatacc gaaagatctc ggaaaagtcg
     3421 aaagcatgga tttcgggcaa ggccctggca ttgtccatat tcagacggcg cacggacatt
     3481 atgaagccca gcagaagatt cgaaaaattc ttcaccatct cgataaaaat tacggcattc
     3541 gtacgttact tgtggaagga agcgctttta aactccggcc tgaaatatta aatttttttc
     3601 cggaagatcc caagcgtacg atgaaagtgg cggatcggct tgcccagcag gcccttgtga
     3661 aaggaccgga actttatctg ctcgatcgaa ttggaaccgc gggagccggg gatcgtgtcc
     3721 ttcggccggc ggaggcgtat ggcatcgaag atgccaggat ctaccgggag aacggggagg
     3781 cctttgttcg ggttctcaca cagaaacaga agacggaaca attcctgcgt gatatggacg
     3841 aaggaattac ccggctttct gcgccgcatt tgagcgactc ccttcggcgg tttatccgta
     3901 cccaggagtc ttttgaaaaa agattgatga gcgtggacgc ctggttttct tacctcaggg
     3961 acgaaacgcg gaaaaatctc ctgcttgatc ttggccaccc ggcgaaccag attgagtggc
     4021 ccatgatggt tcgccttttc aaaatcaggg aacttcagaa aaatctggat ctgcagcaat
     4081 ttgggaaaga acgggaaaaa ttcttgaaag ccgtcgagcg gtttatcccg gaggaaggac
     4141 aggcggccgg aaatgacgac ctagtatccg cggcgcggca ggtgtctgtt cgcccgatat
     4201 cttttttggc tcaaatcgga gaacttcttc agatcgaccg gatggggcaa caattgccgg
     4261 atcctgaaac gagccttttg ttcgaagcca tggttcgtcg tcttccgtcc aattttaatt
     4321 accgccttta tcccaacgtc tgttacttca tcgggattct gcttttgacg agtgaagtga
     4381 aggccgacag gttgatggac gagtcttcca gggtcgcgga aatgattaca tcccggcttg
     4441 cccaaacgga ttctgaaaaa gcgattgtgg cgcttttaaa agactaccgg ctcctccaga
     4501 aactttttgc gttggaactt cttcctgaag attatgaaac gattcttgcg tcttcggcga
     4561 ttggcggttc ttcactttca aacggtcaaa atgttttatc tccttcttcc atcgtgcgtc
     4621 gtcttgaaag tcttgcgagc cagacccctg gcgccggacg ctcgggaaaa gttcaatttt
     4681 ctcaccttga tgaactggac ggattgtttg acagcgcaat gcagttttat gccggggtca
     4741 aggcccgtga tcttgccatg atcggcatgg ttgaaaaacg gttgaaagag acgggggttg
     4801 gtaaggtggc ggttgtgacg ggaggattcc atgcccgccc ttttcgagat tatttttcat
     4861 ctcagggata tacctacgcg ctcgtgacgc caaaaatcac ggatcaggat gaagccggac
     4921 accggcacta cattgaaaat accctccgga tgacccgtgt gggcgcggag ccagcctcga
     4981 atcatcaggc tacgaatcaa gcgtcatctc atttagcgtt tcatgccaga acgtctacac
     5041 gcgaaacggt tccattgatg catactcgtc aggagttgag ggctatggga gtgagagccg
     5101 gtctggtaga gattgctcgc atggcaggcg ttcctcttac cgcgataaac cgggctgaag
     5161 tgcgactcac aaaacaagtc gcggacgaag tgtcccggca agtggggagt tcacagcgcg
     5221 ctcttgagaa cattaaacaa gctccaactt ttgatgaact taacccggat gtccagaatg
     5281 ctttttggga agtttttcaa aatacacgct tgattcccga tgaggcttac ctcgatgact
     5341 ggtatgttca aaatcgtgag ggcgtgattc atgctttgga gaatcctccc
//
LOCUS       DAOSIO010000121         5388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_13608_length_5388_cov_4.336771, whole genome shotgun sequence.
ACCESSION   DAOSIO010000121 DAOSIO010000000
VERSION     DAOSIO010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13608_length_5388_cov_4.336771"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(308..1261)
                     /locus_tag="PK590_05330"
     CDS             complement(308..1261)
                     /locus_tag="PK590_05330"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOG24052.1"
                     /translation="MKKNAKKKQSRRKKTSLFLMTVALIAGLVFLIAQPPVYSLDFER
                     LSDEDIAMRKQVIEKLEPLIRKRDGEKNLSRLTFEELYEPLNKEEREFMEKFRNIPAK
                     EVGVRIPWRGIATGKEALVMIRGQKVKEKVRVKTSDGKRKTVIRTRTIPPQFLPPNVY
                     KQYEKMMAAMEKSIGKRLYVDSGYRSSAYQLYLFVYFLKNHNFSVRETVKFVALPGYS
                     EHGSPKYQAIDFINEDGIDGDPNVKEFEALPENTWLHAHAREYGFVLSYPKDAGKGIT
                     YEPWHWRYDPKAAKKLAKKRSQSAAASRRASSGAYSELAPS"
     gene            complement(1258..2196)
                     /gene="fmt"
                     /locus_tag="PK590_05335"
     CDS             complement(1258..2196)
                     /gene="fmt"
                     /locus_tag="PK590_05335"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOG24053.1"
                     /translation="MKLVFFGSSDFSLKSLDACLASGHETVLVVTTPPQKKGRGLHLE
                     PTVVRVAAEKHGRPVEEFPELCDPEVVRKIRELAPDIFVVASYGKILPKSLLEIPKYR
                     LNVHPSLIPRYRGAAPIHWPILNGDMETGVSIIDIAEKMDAGDIFYQTRYPIHPAMNA
                     AELSHELASLSAQALQAVFRMIEQRSLAGVPQDESQATIARKLVKNDGLVSFRDSAEM
                     ISRKIRGLAPWPGCYFFFKQERVMLLSAVPAVGVTSDPWVPPGTLLEVDPGGSVLVST
                     AEKALRIFRVKPEGKNVMSAFDFVNGRRLSAGQVIL"
     gene            complement(2261..2782)
                     /gene="def"
                     /locus_tag="PK590_05340"
     CDS             complement(2261..2782)
                     /gene="def"
                     /locus_tag="PK590_05340"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOG24054.1"
                     /translation="MSLLKLRTYPDPVLKRQAAPHQTFGPLDQKLFDDMIETMYRDDG
                     VGLAAPQVGISKRIFVCSPRMTRGEEYVMVNPVIEKATGAAVAAEGCLSLPGISADVE
                     RATEVRLTYQDRSGRLHRIEVYDFFARVVQHEMDHLNGKLLIDHFTGKKREELLGQYE
                     KTRTAGDVSKLQD"
     gene            complement(2821..5043)
                     /gene="priA"
                     /locus_tag="PK590_05345"
     CDS             complement(2821..5043)
                     /gene="priA"
                     /locus_tag="PK590_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOG24055.1"
                     /translation="MMNSTSLLQPKETSVLIAQVAIPLPIATEVFSYSVPEAWAEKAV
                     PGVRVLVPFRNREITGYVVRLDLASASDSRHVPEKQNQTLKKLKPLIAVLDDEPVLSE
                     NMLDLTKWVADYYGSSWGEAIENALPRWVKYGKKTAKYIEKAKKKTNAGSSAVTQPKE
                     FKLTDEQKEAFEAIRKALDNGCDLPSREKNKAQAPINHASLPILLFGVTGSGKSELYI
                     RAIREVLKKGRGAICLVPEIALTEQLKRFFTGHFGGDLEILHSKMTDGERFLAWKRIE
                     MGTARVVLGPRSAVFAPVPDLGLIVMDEEHEGSYKQETAPRYHAREVAAQRARHEGAL
                     FIMGTATPSLESMRAAETGEFTRLDLTRRIDHKAMPAVTVIDLKRYEGEKRASLILSP
                     PLMKEIGKNLSASEGTMLLLNRRGFSTSLRCRECGETEKCKSCQVSLTYHQEKGILLC
                     HYCNYQKKVPETCSSCGAPILKFMGFGTEKVESEVAKFFPGARIARLDADTTRKKDSH
                     EVILSGFRDRKIDVLVGTQMIAKGFDFPHVTLVGVVLADIGLRLPDFRSSERTFQLLT
                     QFAGRAGRGVKPGRVLIQTYMADHASIHHAMTHNFPGFYAHETRFREEFRYPPFCSLV
                     NIIVRSRDEKKAYLFAREVRDALNGARRTDGDKPGSESSSILHSLPGSVEIIGPAPLP
                     FYKLRGHYRWHVMLKLPRPVSGEGSAVLMPGKIQSILLSLKKSSAVAFALDVDPLNIL
                     "
BASE COUNT         1261 a         1491 c         1338 g         1298 t
ORIGIN      
        1 ttgatccatg aataatgacg tttaaaccga tccaacctcg caggttggat cggttccgca
       61 aaacggcgag agggcggaaa tataccccat ccatcaagaa atttcactta aaatttatta
      121 aagggctaat ccatcttgtc ttttcctaac ggtctcccgc ttgaattcct tggacagttt
      181 cttgccgcga accggaacaa acacaccatt tacgccctgc gaggttggat cggtcgcgtg
      241 cctttcaagc cggagtgaaa cgaaacatgg cgggaatctt tgatcgatat caaaaacggg
      301 attcgagtca ggacggggcg agttcggaat aagccccgga ggacgcgcgt ctggaagcgg
      361 cggctgattg tgagcgtttt ttggccagtt tcttcgcggc cttcggatca tagcgccaat
      421 gccacggctc gtaagtgatt cccttgccgg cgtccttcgg gtaggaaagt acaaacccgt
      481 actcgcgggc atgcgcgtgg agccaggtgt tttccggcaa ggcttcaaac tctttcacgt
      541 tcggatcccc gtcaatcccg tcttcattga tgaaatcgat cgcctggtat ttcggggaac
      601 cgtgctcgct gtaacccggc agagcgacaa atttaaccgt ttcccggacc gagaaattgt
      661 gatttttgag aaaataaacg aaaaggtaga gctgatacgc gcttgaacgg taaccggaat
      721 caacatagag ccgtttgccg atgctttttt ccatggccgc catcattttt tcatactgct
      781 tataaacgtt cggaggaaga aactggggag ggatcgttcg ggtcctgatc acggttttcc
      841 ttttgccgtc ggaagtttta acacgaactt tctctttcac cttttgcccc cggatcataa
      901 cgagcgcttc tttacccgtt gcgatccccc gccacgggat acggacgcca acctctttcg
      961 cgggaatgtt ccgaaacttt tccataaatt cccgctcctc cttgttcagg ggctcataaa
     1021 gttcttcaaa agtcagtctg gaaagatttt tttctccgtc gcgttttcgt atcaggggtt
     1081 ccagcttttc gatcacctgt tttcgcatcg cgatatcctc atccgacaga cgttcaaaat
     1141 ccagagaata gacgggcggt tgggcgatca gaaaaaccaa tcccgcgatc aacgccacgg
     1201 tcatcaaaaa cagggatgtt tttttccggc gagattgttt ttttttggca ttctttttca
     1261 caaaatcacc tgtcctgccg aaaggcgacg gccattcacg aaatcaaacg cgctcatgac
     1321 attctttcct tccggtttga cgcgaaaaat acggagggct ttttccgcgg tggacacaag
     1381 caccgaaccg cccggatcca cttcaaggag ggttccgggt gggacccacg gatccgacgt
     1441 aacgcccacc gcgggaaccg cggaaagaag catgacccgt tcctgtttaa aaaagaaata
     1501 acaaccgggc cacggggcaa gcccgcgaat ttttcgtgaa atcatttccg ctgaatcccg
     1561 gaaagaaacg agaccgtcat tcttgaccag tttccgggcg atcgtggcct gggattcgtc
     1621 ctggggaacc cccgcgagcg accgttgttc gatcatcctg aaaacggcct gaagcgcctg
     1681 ggcggacagt gaagcaagtt catgggacag ctccgcggca ttcatcgcgg gatggatcgg
     1741 ataacgtgtc tgatagaaga tgtcgcccgc gtccattttt tccgcgatgt caataatgct
     1801 gaccccggtt tccatatctc cgttcagaat gggccaatgg atcggcgcgg cgccccggta
     1861 gcgagggatc agggacgggt gaacgttcaa acggtatttc ggaatttcga gcagactttt
     1921 cgggagaatt tttccgtaag aagccaccac aaaaatatca ggagcaagtt cccggatttt
     1981 tcgaacaact tcgggatcgc aaagttccgg aaattcttcg acgggtcgcc cgtgtttttc
     2041 cgcggcaaca cgcaccacgg tcggttccag gtggagacca cgtccctttt tctggggcgg
     2101 cgtcgtgacg acaaggactg tttcgtgccc tgaggcaaga cacgcgtcga gagactttaa
     2161 tgaaaaatca ctggagccaa aaaatacaag tttcatggaa atccatcccg agttctaaat
     2221 ccggaatccg gctcaaatcc tgaaattaca ggttcgagat tcagtcctgg agcttggaaa
     2281 cgtccccggc ggttcgtgtc ttttcgtatt gtcccaatag ctcttcccgc ttctttcccg
     2341 taaaatgatc gatgagcaat tttccgttca ggtggtccat ttcatgctgg accacgcgcg
     2401 cgaaaaaatc ataaacctcg atccggtgga gccgtccgga ccggtcctga taggtcagcc
     2461 gaacctcggt cgcccgttcg acatccgccg aaatccccgg cagactgagg caaccttcgg
     2521 cggccacggc ggccccggtg gctttctcaa tgacgggatt caccatcaca tactcttctc
     2581 cgcgggtcat acggggcgag caaacaaaaa tccgtttcga gatcccgacc tggggagccg
     2641 cgagccccac accgtcatcc cgatacatcg tctcgatcat atcgtcaaaa agcttttgat
     2701 cgagcgggcc aaacgtctgg tgaggagccg cctgtcgctt taaaaccggg tccggatatg
     2761 tgcgtaattt cagaagtgac atcgtgggcg gattataaca tatcgccttg ctccgtacgg
     2821 ctaaagaatg ttgagagggt caacatccag cgcgaacgca accgcggaac tttttttgag
     2881 agacagtaaa atcgactgga tttttcccgg catgagaacc gcgctccctt cccccgaaac
     2941 gggccgcgga agttttaaca tcacatgcca ccggtaatga ccgcggagct tataaaacgg
     3001 caagggcgcg ggaccgatga tctcgacact ccccggcaag gaatggagga tggaagatga
     3061 ttcagaacct ggtttgtcgc catcggttcg ccgcgctccg ttaagcgcgt cccggacttc
     3121 cctcgcgaac aggtaggctt ttttttcatc acgcgaccgc acgatgatat tgacgagtga
     3181 acaaaacgga gggtatctaa actcctcgcg gaaacgtgtt tcgtgcgcgt aaaatcccgg
     3241 aaagttgtgg gtcatcgcgt gatgaatgct ggcgtgatcc gccatgtaag tctggatcaa
     3301 aacgcgaccg ggtttgacgc cccgtcccgc ccggcccgca aactgggtca aaagctggaa
     3361 agtcctttca ctcgaacgaa aatcgggaag acgcaagcca atgtccgcca gcaccacgcc
     3421 aacaagcgtc acgtgtggaa agtcaaatcc cttcgcgatc atttgcgtgc caaccaggac
     3481 gtcaatcttg cggtcgcgaa atccggaaag aatgacttca tgagaatcct tttttcttgt
     3541 ggtatcggcg tccagtctcg cgattcgggc gcctgggaaa aatttcgcga cttcactttc
     3601 gaccttttcg gttccaaacc ccataaattt caagatcggc gcgccgcagg acgaacaagt
     3661 ttccggaact tttttctgat aattgcagta atgacaaaga aggatcccct tttcctgatg
     3721 ataagtcaat gaaacctggc aggacttgca cttttccgtt tctccgcatt cacggcatcg
     3781 aagggacgtg gaaaaacccc gccgattcaa aaggagcatt gtcccctcac tagccgacaa
     3841 attttttccg atttctttca tcagcggcgg agaaagaatc aggctggcgc gtttttcacc
     3901 ttcgtaacgc ttcagatcga tgacagtcac ggccggcatg gccttatgat caattctccg
     3961 ggtcagatca aggcgcgtga attcgcccgt ttccgcggca cgcatgcttt ccagagacgg
     4021 cgtcgccgtc cccataatga acagcgctcc ctcatggcgc gctctctgag cggccacctc
     4081 acgggcatga taccggggcg cggtctcctg tttataggaa ccttcatgtt cttcgtccat
     4141 cacaatgagc ccgagatccg gcaccggcgc gaacacagcc gagcgcggac cgagaacgac
     4201 acgggcggtt cccatttcga ttcttttcca cgccaaaaac cgctcgccgt ccgtcatctt
     4261 gctgtgcaaa atttccagat ctccgccgaa atgccccgta aaaaaccgtt tcagttgttc
     4321 ggtcagcgca atttccggaa caagacaaat cgcgccccgc ccttttttga ggacttccct
     4381 gatggctcga atgtagagtt cacttttccc gcttccggtc acgccgaaaa gaagaatggg
     4441 taaagatgcg tggttgatgg gcgcctgagc cttgtttttt tcgcggctgg gcaaatcaca
     4501 tccgttgtcc agcgcttttc gaatcgcttc aaacgcttcc ttttgttcgt cggtcagctt
     4561 aaattctttc ggttgagtga ccgcggaaga tccggcgttt gttttctttt ttgccttttc
     4621 aatgtatttc gcggttttct tcccgtattt tacccagcga gggagcgcgt tttcgattgc
     4681 ctctccccag ctagagccat aataatcggc aacccatttt gtaagatcca gcatgttttc
     4741 cgacaggacg ggttcatcat ccagcacggc gatcagcggt tttaactttt tcagcgtctg
     4801 gttctgcttc tccggaacgt gtcgggaatc ggaggctgaa gcaagatcca ggcgaacaac
     4861 ataacccgtg atctcacggt ttcggaacgg gacaagaaca cgcactccgg gaacggcctt
     4921 ttcagcccat gcttcgggaa cagaatagct aaagacttcg gtcgctatgg gaagagggat
     4981 tgctacctgg gcgatcagga cactggtttc tttcggctgg agcaaagaag ttgaattcat
     5041 cattgatgtc aaatcgaaaa agcagtcatt gcttataatt atgggtcatt aaaatcagta
     5101 tcctagtcgt catcctgaac aacgccacca aatcctcgtc atcctgaacc acatccccaa
     5161 tcctcgtcat cctgaacgaa gtgaaggatc tctccttccc caagatgctt cgcttcgctc
     5221 agcatgacga gccttgcgtc aaccaatcgt caccctatgc cacgccccaa cccccgtcat
     5281 cctgagcccc cccaaatccc cgtcatcctg aacgaagtga aggatctctc tttcccaaga
     5341 tgcttcgctt cgctcagcat gacgagcctt gcgtcaacca atcgtcac
//
LOCUS       DAOSIO010000122         5360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_13695_length_5360_cov_4.267861, whole genome shotgun sequence.
ACCESSION   DAOSIO010000122 DAOSIO010000000
VERSION     DAOSIO010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13695_length_5360_cov_4.267861"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(526..1062)
                     /locus_tag="PK590_05350"
     CDS             complement(526..1062)
                     /locus_tag="PK590_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HOG24056.1"
                     /translation="MVVMNGETKKMTTKLIPQEIIQSKIFLLRGQKIMFDKDLAKLYG
                     VATKVLNQAVKRNIDRFPSDFMFLLTPQEVTSLRSQFVTSTRGGLRYQPCAFTEHGIL
                     MLSSVLNSKRAIQVNIQIMRAFTKLREMLVSNKELRQKIEEMEKKYDHQFKIVFQTIK
                     ELLEPPTPKTKGPIGFHG"
     gene            complement(1050..2567)
                     /locus_tag="PK590_05355"
     CDS             complement(1050..2567)
                     /locus_tag="PK590_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOG24057.1"
                     /translation="MIRIKSAFALGIHAFPVDIEVDIGGGIPQLILVGLPDASIKESR
                     ERVRTAIKNSGYQLSQEKITINLAPAELKKEGAAFDLPIALGILAGHGIVPPERLNEF
                     IFLGELALDGRLRPFRGTIAVTASFRGQSFVLPHENSDEASTVKNTVAYPVKSLNEAV
                     SFLRGETEIKPVTWRETKRELSPLSCLDFSEVKGQRAAKRAIEIAVAGGHMIMLIGPP
                     GSGKTMLARRIPSILPPMDSDEALEVTKIHSAAGITVHRPGLIWERPFRSPHYTASAV
                     ALAGGGSDPRPGEMSLAHNGILFLDEFPEFRRDALETLRGPLEDGTITVSRAKMQITY
                     PARFLLVAAMNPCPCGHLSDPHKSCRCSLGQIHKYRSKLSGPVLDRIDIQIEVPALSF
                     QTLSNQEIPEPSQAIRDRICACRNIQKKRFRLRPCQTNSQMSPRDIKNHATPDAQGGK
                     LIESAMKNLRLSARAYYKILKIARTISDLAGCEQIQSEHIAEAIQYRSLDRQWWS"
     gene            2729..3202
                     /locus_tag="PK590_05360"
     CDS             2729..3202
                     /locus_tag="PK590_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HOG24058.1"
                     /translation="MSSDSILMMTPDISHCEPTKAFFAVFLSWFVAQALKVALGGTRT
                     KKFDFRWLFDTGGMPSAHSATVSSLATVTGLYYGFNSIPFLITLIVCLITMFDAAGVR
                     RHFGRQASILNQMLNDFYVKGEVPEKRMKELLGHTPFEVFAGALVGIIVGVLFCG"
     gene            3206..4054
                     /locus_tag="PK590_05365"
     CDS             3206..4054
                     /locus_tag="PK590_05365"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOG24059.1"
                     /translation="MKSQMIITLIVLGFFLTGGPAFAEDVSPEFLAEQPTEFETDSSE
                     NPEVPDQEFASQALENLQEETVEVVSLSQEEKPVELLTDLAQIALRTSPAQMKQNLDK
                     IFVSKTAAGIFSYTIQSGDSLYVLARKNNTTVDLIRKLNHLEKDMIYPGDEIKIFSGD
                     FSILVDKSRNRLTLFADGQAVKEYKVSTGKNNSTPVGEFKITDKLVNPTWFKSGAIYA
                     PGSPDNELGTRWLGFDKPGYGIHGTIEPEKIGTQASEGCIRMLNQDVEEIDSLVPSGA
                     RVVIQD"
     gene            4168..5217
                     /locus_tag="PK590_05370"
     CDS             4168..5217
                     /locus_tag="PK590_05370"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOG24060.1"
                     /translation="MAKKNKKTKTKTADNEPILPAGKIQIGTRDIFDICLPFERAVVD
                     MEKKINELKKISQSGVDLSEEIKSAEKKLKDAIGEVFDNLTPWQRVQVARHPLRPYTL
                     DYINYLTTDFVELHGDRHYSDDRAIIAGFAKWGGKEPICVIGHQKGRDTKENLMRSFG
                     SAHPEGYRKAMRIMRLAERHHVPVLCFIDTPGAYPGIGAEERGQAEAIAYNLREMGDL
                     RIPIVCIVIGEGGSGGALGIGVGDRVYVLENAYYSVISPEGCAAILWKEPTRAPDAAT
                     ALKLTGPDLLELGVIDGVIEEPLGGAHRDFERTAENIRLTVDKALAELKEIPRKDLPE
                     ERYQKYRRMGVFTSK"
BASE COUNT         1319 a         1204 c         1417 g         1420 t
ORIGIN      
        1 ggatgccttg tcgcgtccag ggcaggattt cttcgcgttt gtccgcaaaa tcaaggacag
       61 tgacgttctg ataaggcacc cggatgtgtt tgaggagaat cggcagggta cacttaccga
      121 cagaaccgaa cccaagacaa agaattctct gattgaaggt tatcatgccg gaaacctcct
      181 ttgaatgatc cgtaagtcgt gacgattttg cggcttataa gtgtttcggg ggcattatgc
      241 aaaagataat ccataagtaa aatatatata tattattttt aatttagatt ttatgtatag
      301 ggtggatttt gcctgttttc caggaaaata ttttttcccg caggagtttt gtgtcggcaa
      361 aaggatttga attcttgggg cggttatggt ggtagtccgc cgaaagcctt tcagaacaat
      421 cgagattgtt aatggaggat caagattttc aagaattgta tagcctgtta tgatgacggc
      481 gatggtaagc cttggggacg gttctcgcca tagctttctg ccagttcaac catgaaaccc
      541 gatcgggcct ttggttttgg gggttggggg ctcgagtaat tctttaattg tctgaaacac
      601 gattttaaat tgatggtcat atttcttttc catttcttcg attttctgcc gcaactcctt
      661 attgctcacc agcatttctc gtaatttagt gaaagctctc ataatttgta tgttgacttg
      721 gatcgctcgc ttgctgttta aaacgctgga aagcatcagg atcccatgct ctgtaaaggc
      781 gcatggttga tagcgtaatc caccccgagt tgaggtcaca aattgtgacc tcaaacttgt
      841 aacctcttgc ggtgtaagta gaaacataaa gtcggacggg aatcgatcaa tgttgcgctt
      901 tacagcctgg tttaacacct ttgtcgcgac cccgtatagt ttcgccaagt ctttatcgaa
      961 catgattttt tgtcctcgga gtagaaaaat cttgctttgg ataatttctt gaggaataag
     1021 ttttgttgtc attttttttg tttctccgtt catgaccacc attgcctgtc cagactccgg
     1081 tattggatag cttccgcgat atgttcggac tggatttgct cgcaaccagc caagtcactg
     1141 atcgtccggg cgattttgag gattttgtaa taggccctgg cggagaggcg caggtttttc
     1201 atcgcgcttt caatcagttt tccgccctgg gcgtctggcg tcgcgtgatt tttgatgtct
     1261 ctggggctca tttgcgagtt tgtttggcag ggacgcaggc gaaatctttt tttctgaatg
     1321 ttccggcagg cgcagatccg gtctcgaatc gcctgggacg gttcggggat ttcctgattt
     1381 gaaagagttt gaaatgagag cgcgggcact tcgatttgaa tatcaattct gtcgagcact
     1441 ggtcccgaga gcttggagcg atacttgtgg atttgcccaa gtgagcaacg gcaggacttg
     1501 tgaggatccg atagatgtcc gcacgggcag gggttcatgg cggcaacaag cagaaatcgc
     1561 gccggatagg tgatttgcat tttcgcgcgg gacacggtaa tcgtaccatc ttccaggggg
     1621 ccgcgaagcg tttcgagggc atcgcgcctg aactcaggaa attcgtcgag aaaaagtata
     1681 ccgttgtggg caagcgacat ttccccgggt ctggggtcgc tgcctcctcc ggcgagcgcg
     1741 accgctgaag ccgtgtaatg aggggaacgg aagggcctct cccatatgag gcccggacga
     1801 tgaactgtga tcccggcggc actatgaatt ttcgtgacct caagggcttc gtcggaatcc
     1861 atgggaggaa gaatacttgg aatgcgacgg gccagcatgg ttttgccgct tcccggagga
     1921 ccgatcaaca tgatcatgtg ccctccggcg actgcgattt caatggctcg cttggcagct
     1981 cgctggccct tgacctcgga aaaatcgaga caagagagcg gggataattc tcttttggtt
     2041 tccctccatg tgacgggttt aatttccgtt tcccctcgca gaaaggagac ggcctcgtta
     2101 aggcttttaa ccggataagc gacggtgttt ttaacggtag aggcctcatc ggagttttca
     2161 tggggcaaca caaaggattg gcctctgaaa cttgccgtga ccgcgatggt tccccggaaa
     2221 gggcggagcc gcccatctag ggcaagctca ccgagaaaaa taaactcatt taacctttcg
     2281 ggtgggacaa tcccgtgacc cgcgagaatt ccaagcgcga ttgggagatc aaacgcggca
     2341 ccttcttttt tcagttcagc gggcgccagg ttgattgtaa ttttctcctg agaaagttga
     2401 taaccgctgt ttttgatggc ggtacgcact ctttcccgtg attccttaat acttgcgtca
     2461 ggaagtccga cgaggataag ctgggggatg cccccgccga tgtcgacttc aatatcaacc
     2521 gggaaggcat ggatccccaa tgcgaaagcg cttttaatcc ggatcacggc agtttctcct
     2581 tttagctctt gagacagcat gtgggaaagc taaaccttgc tttttaaaat accaaaatct
     2641 ataataaccc tacgaaacaa accctgtggg tttggtatta ttcatttaaa taatattata
     2701 tattcaaata aaacatatca ttaaaggatt gtcaagcgat agtattctga tgatgacacc
     2761 cgatatttca cattgcgagc ctaccaaggc tttttttgcc gtttttctca gctggtttgt
     2821 cgcccaggcg ctgaaagtag ccctgggtgg tacgcgaacg aaaaagtttg attttcgttg
     2881 gctgtttgac accgggggga tgccgagcgc gcattcggcg actgtgtcga gcctggccac
     2941 cgtgacgggt ctctactatg ggttcaatag tatccctttc ctgatcacat tgatcgtttg
     3001 tctcatcacc atgtttgacg cggccggtgt gcggcgccat tttgggcgtc aggcttcaat
     3061 cttaaaccag atgctgaatg atttttatgt taaaggggaa gttcccgaaa aaagaatgaa
     3121 agaattgcta ggacacacgc cttttgaagt ttttgccggc gctctcgttg gcattatcgt
     3181 gggtgtcctt ttctgcggat aatttatgaa atctcaaatg attattaccc tgattgtgtt
     3241 gggatttttt ttaacgggcg ggcctgcttt tgccgaagat gtgtctcctg agtttttggc
     3301 tgagcaaccg acagaattcg aaacggacag ttccgagaac ccagaggttc cagaccaaga
     3361 gttcgccagc caggcccttg aaaatcttca ggaggaaact gttgaggtcg tgtctttaag
     3421 ccaggaagaa aagccggtcg agcttttaac cgatcttgct caaattgcct tgcgaaccag
     3481 tccggcgcaa atgaaacaga acctggataa gatctttgtt tcaaaaacag cggcaggaat
     3541 tttttcttat acgatccagt cgggcgacag cctttacgtc ctggcgagaa aaaacaatac
     3601 aacagttgac ctcatccgga aacttaatca tctcgaaaaa gacatgattt acccgggaga
     3661 cgaaatcaag attttttcgg gtgatttttc aatcctggtt gataagtcaa gaaatcgcct
     3721 cactcttttc gcggatggtc aggcggtcaa ggaatataag gtttcgacag gtaaaaacaa
     3781 ttcgacgccc gttggtgagt tcaagattac ggacaagctc gtgaatccaa cctggtttaa
     3841 atcgggagcc atttacgcgc cgggaagtcc cgacaacgag cttggtaccc gctggcttgg
     3901 atttgataaa ccgggatatg gaattcatgg gacaattgag cctgaaaaga taggaacgca
     3961 ggcttctgaa gggtgcatcc gaatgctgaa tcaggatgtg gaagaaattg atagtctggt
     4021 tccttctggc gcccgtgttg ttattcaaga ttaatccggt tttgactacg agagggtgag
     4081 ttctccctgg gaagggacgt tttaaaggga gcttcgcgcg ccaccggaat ttgccgggaa
     4141 gtgaacaaac tcgaaaagaa ggatcttatg gcaaagaaaa ataaaaaaac aaaaaccaaa
     4201 acagctgaca atgagccgat tctccccgcg gggaaaatcc agatcggaac ccgtgacatc
     4261 tttgacatct gtctcccgtt tgaacgcgct gtcgtggata tggaaaaaaa gattaacgag
     4321 cttaaaaaaa tcagtcagag cggcgtcgac ctttccgagg aaatcaaaag cgcggaaaag
     4381 aaactcaaag acgcgatcgg agaggttttt gacaatctta ctccgtggca acgtgtccag
     4441 gtggctcgtc atccccttcg cccgtataca ctggattaca ttaattatct cactacggat
     4501 tttgtggagc tccatgggga ccgccactat agcgacgacc gggccattat tgccggattt
     4561 gcaaaatggg ggggcaagga gccgatatgt gtgattgggc accagaaggg acgcgacacc
     4621 aaggaaaatc tgatgcgcag tttcggaagc gcgcaccctg aaggataccg aaaagcgatg
     4681 agaatcatgc ggcttgcgga gcggcatcat gttcctgtgc tttgctttat cgatacgccg
     4741 ggcgcttatc ccgggatcgg cgccgaagag cgcggccagg ctgaggctat tgcgtacaat
     4801 ctgcgcgaga tgggtgattt gcgaatccct atcgtctgca ttgttatcgg cgaagggggg
     4861 agcggtggcg ctcttggtat cggagttgga gatcgggttt atgttcttga aaatgcctac
     4921 tactcagtga tatctccgga aggttgcgcg gccattctct ggaaagagcc gactcgcgcg
     4981 cccgacgccg cgaccgcgct taagctgaca ggcccggatc ttcttgagct tggtgtgatc
     5041 gacggtgtta tcgaggaacc gctcggaggt gcccaccgcg attttgaacg cacagccgag
     5101 aacatccggt taaccgttga caaggctctt gccgaactga aagaaattcc gagaaaagac
     5161 ctgcctgaag aacgatatca aaagtatcga agaatgggtg tttttaccag caaataaaat
     5221 gtcggagttt cacttctcgc tgggtgtgac ctgcgagagc gtaaaaacgg gcacgatatc
     5281 tgttaagcgg attcgttaag tagccctccc gccttcaacc caacatctcc agtttcatca
     5341 gccaggcctt tacgtctcag
//
LOCUS       DAOSIO010000123         5100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_14614_length_5100_cov_3.658276, whole genome shotgun sequence.
ACCESSION   DAOSIO010000123 DAOSIO010000000
VERSION     DAOSIO010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14614_length_5100_cov_3.658276"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PK590_05375"
     CDS             complement(<1..312)
                     /locus_tag="PK590_05375"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HOG24061.1"
                     /translation="MSCKTLADGIHHFTCFPSFVRAGFSSREFPRARYEDFLTGLSLK
                     MDDLVMVEQVHGREIFVAEAPTRGRMEVEADGLVTDRVGLVLGIRTADCVPIFFSSER
                     TR"
     gene            complement(309..2492)
                     /locus_tag="PK590_05380"
     CDS             complement(309..2492)
                     /locus_tag="PK590_05380"
                     /inference="COORDINATES: protein motif:HMM:NF015390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOG24062.1"
                     /translation="MKTISSKNKANVFLLMALALLVSGCNQGTQVFAQGGPVEGTTSP
                     DRSSGGPTLGEVEMVADFETGTLTNNRGGASGDWNWNPADVNNSYTDATIVSMPGADG
                     IESRVLQLTYSVDSDVPARNGFWTKLMAVDGRKYDHLALDIKGDPKRGFTEKFRIEVK
                     KCLDEECQQTIQGSAVISVSEEWETVNVPLNKLTGLIDFSDPDAWTDPKKSYDHLDEL
                     IIIFNDVFVTKKSGRIFIDNIRFLNTGKPGPSAVDQPPRTESKTELKHDTLEYQRFLI
                     TRLRGFPEKVRVSREFPQEEREFLIEVARDTWKFFDEVIDVQNHLPLDNITLGEEQPD
                     GEGLWIGDYTNVTNVGIYLMSVVSAYDLGFISRENAVRRIRETLGAVEKLEYHASGFP
                     YNYYDTTVLDKTSYFVSFVDSGWLLLGLYVAKAAFPEELDEQATRLLGRGDLRFFYDP
                     VEKQMYHGYYANLDVYSDYHYGVFYAEPRAASYMAIARGDVPEEHWFSGPIRTFPVHY
                     SWQSQTPKNRVEKTVLGHTVNGGYYEWKDLQYVPSWGGSAFEALMPTVVLNEKELAPE
                     GLGANNAVHALGQYRYATEELNQPVWGMSPCSVPGGTYAEYGAKAFGIKGYKPGVVTP
                     HASVLTLEYVPKEAVANLRKMIELYDIYGEYGFYDSVDIESGKVARKYYALDQGMILV
                     AINNYLGDGPIRRRFHSNPEMKNAEKLLSEEKFFKETETVSSPES"
     gene            complement(2489..3574)
                     /locus_tag="PK590_05385"
     CDS             complement(2489..3574)
                     /locus_tag="PK590_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOG24063.1"
                     /translation="MTTRTVSRFMRQRISIFIILAFCTSFFSAPFPPAEAAYLPLSAR
                     SFVVMDARTQKVLYSKSPHAKCAPASTAKLLSVMVILDHLDPDQVVRVPHYATQVQPS
                     KIHIASGEQFYVRDLIKAMLLCSANDAAYALAIRSAGSVKSFSSRMNAKARRIGAKNS
                     HFISPAGLPASGQYSTAYDLARIMAAAQKYPLILQNMKQRGATIRSLNGRTFYLKNTN
                     KMLWDTGSREIIGKTGYTRAARHCFAGRIRIGSKQMLVGLMGAPRRANLWSDLKKMAA
                     FPPSIGDSDKKKTKDPILVNRQLNSREKVKQIQTALKRAGYFKGPVNGNFGPMTLAAT
                     QKFQRAKGLNADGIVGVKTWTKLKVYL"
     gene            complement(3629..3702)
                     /locus_tag="PK590_05390"
     tRNA            complement(3629..3702)
                     /locus_tag="PK590_05390"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3666..3668),aa:Pro,seq:tgg)
     assembly_gap    4266..4365
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4658..5089)
                     /locus_tag="PK590_05395"
     CDS             complement(4658..5089)
                     /locus_tag="PK590_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015421460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24064.1"
                     /translation="MRLKSFFLVLLFASLFFSASGCATSQQQILSTDQSQVALRSIQS
                     RAFDTTDKKLMMRSVINTLQDLDFLIQDSSLDLGTVTGQKFHGITTVKMSVTVKERNE
                     KQLLVRANAQFGLKAVEDPKTYQDFFVALEKSIFLTAHQVD"
BASE COUNT         1236 a         1430 c         1130 g         1204 t
ORIGIN      
        1 tctggttcgt tctgacgaaa aaaagatggg gacgcaatcc gctgtgcgaa taccaagtac
       61 cagcccaacc cggtcggtca ccaacccgtc cgcttcaacc tccatccgcc cgcgagtggg
      121 cgcctccgcg acaaaaatct cacgcccgtg aacctgttcg accattacca gatcatccat
      181 tttgagcgac aggccggtca aaaaatcctc gtagcgcgcg cggggaaact cgcgcgaaga
      241 aaatcccgcg cgaacaaagg aagggaagca tgtgaaatgg tggataccgt ccgccagagt
      301 cttacaactc atgattccgg tgaagaaacc gtttctgttt ctttaaaaaa tttctcttca
      361 cttaaaagct tctccgcgtt tttcatctcg ggattggagt gaaagcgtct gcggataggg
      421 ccgtctccca aatagttatt gatcgcgaca agaatcatac cctggtcaag cgcgtaatat
      481 ttacgcgcga ctttgccgct ttcaatatcg accgaatcgt aaaacccgta ctccccgtaa
      541 atatcataga gctcgatcat ttttctgaga ttggcaacgg cctctttggg cacatattcc
      601 agcgtgagca ccgacgcgtg aggcgtgacc acaccgggtt tataaccctt gataccaaac
      661 gccttcgccc catattccgc atatgtaccg ccgggaaccg agcaggggct catcccccac
      721 acgggttgat ttaattcctc cgtggcataa cgatactgtc caagcgcatg aaccgcgttg
      781 ttggccccaa gcccctcagg ggccagttct ttctcattca gaaccaccgt cggcataagc
      841 gcctcaaagg ctgatccgcc ccaacttggg acgtattgaa gatccttcca ctcgtaatat
      901 cccccgttca cggtgtgtcc aagaacagtt ttttcaaccc ggtttttggg agtttgcgac
      961 tgccagctgt aatggacggg aaaagtccgg attggtcccg aaaaccagtg ttcctcggga
     1021 acgtctcccc tcgcgatcgc catataagac gccgctcgcg gctcggcgta aaaaacaccg
     1081 tagtgataat ccgaatacac gtcgagattg gcgtagtagc cgtggtacat ctgtttttcc
     1141 acgggatcat aaaaaaaccg cagatccccg cgccccagaa gccgggtggc ctgctcatcc
     1201 agttcttccg gaaacgcggc ctttgccaca taaagcccga gcaggagcca gccactgtcc
     1261 acaaacgaca cgaagtaact ggtcttgtcg agcactgtgg tgtcatagta attgtacggg
     1321 aaaccgctcg cgtgatattc gagtttctca accgcgccca gtgtttcacg gatccggcga
     1381 acggcatttt ctcttgagat aaaaccaagg tcgtaggcgc tcaccaccga catcagataa
     1441 atgccgacgt tcgtcacatt ggtgtaatcc ccgatccaga gaccctcccc gtccggttgc
     1501 tcctccccaa gtgtgatatt gtccagcggc aggtgatttt ggacatcaat cacctcgtca
     1561 aaaaatttcc aggtatcacg ggcgacctct atcaaaaact cccgttcctc ctggggaaat
     1621 tcccgggaaa cacggacctt ctccggaaaa ccccgaagcc gcgtaatcaa aaatctttga
     1681 tactcgagag tgtcatgttt caactcggtc ttggactccg tcctgggggg ttggtccacg
     1741 gccgaaggcc ccggttttcc ggtatttaaa aagcggatat tatcgataaa aattcttccg
     1801 ctttttttcg tcacaaagac gtcgttaaaa ataatgatca attcatccaa gtgatcatag
     1861 ctttttttag gatctgtcca cgcgtcaggg tccgaaaaat caatgagccc cgtcagcttg
     1921 ttcaaaggaa cattcaccgt ctcccactcc tcgctgaccg aaatgaccgc cgaaccctgg
     1981 atcgtctgct gacattcctc atccaggcat ttcttcacct caatcctgaa cttttccgta
     2041 aacccgcgct tcggatcgcc tttgatgtcc agcgccaggt gatcatactt gcggccatca
     2101 accgccatta actttgtcca aaacccgtta cgcgccggca catccgaatc cacgctgtaa
     2161 gtaagctgaa gcacgcggga ttctatcccg tcagcccccg gcatgctcac aatcgtcgcg
     2221 tccgtgtagg aattattcac gtcggccggg ttccagttcc agtccccgct cgcgcctccc
     2281 ctgttattcg ttagggttcc tgtttcaaaa tcagcgacca tttcaacctc ccccagagtt
     2341 ggtcctccgg aagaacgatc aggcgacgtc gtcccttcga ccggtccacc ctgcgcgaaa
     2401 acctgggttc cctggttaca accggataca agcaaggcaa gcgccatcaa caaaaaaaca
     2461 ttcgctttgt tttttgaaga gattgttttc ataaatacac ctttagttta gtccaggttt
     2521 tgacgcccac gatcccgtcg gcgttaagtc ccttggcgcg ttgaaatttt tgagtcgcgg
     2581 ctaacgtcat cgggccaaaa ttcccgttca ccggcccctt aaaataaccg gcccgcttga
     2641 gggccgtctg aatttgtttg accttttcac gagaattcag ttgccggtta acaagtatcg
     2701 gatcttttgt ttttttcttg tctgaatcac caatcgacgg cgggaaggcc gccattttct
     2761 tcaggtcaga ccaaagattg gcgcgccggg gagcccccat cagtcccacc agcatttgtt
     2821 tgctaccaat ccggatccgg ccggcaaaac agtgccgagc ggcccgggtg taacctgttt
     2881 tcccgataat ttcccgcgat ccggtgtccc aaagcatctt gttcgtattt ttcagataaa
     2941 acgttcgacc gttcagactg cggatcgtcg cgccgcgttg tttcatgttt tgaagaatta
     3001 aaggatattt ttgggccgcg gccataattc gcgccaggtc ataagccgtt gaatattgac
     3061 cgctcgccgg caatccggcg ggacttataa aatgagagtt tttggcgcca atccttcgcg
     3121 ccttcgcgtt catccgtgaa gaaaaacttt ttacggaccc ggccgaccgg atggcaaggg
     3181 catacgcggc gtcattcgcc gaacaaagca acatcgcctt gatcaggtca cgcacataaa
     3241 attgttcccc cgacgcgata tgaattttag aaggttgaac ctgtgttgcg taatgcggaa
     3301 cgcggaccac ctgatcgggg tcaagatgat ccagaataac cataactgaa aggagtttcg
     3361 ccgtacttgc cggagcgcat ttcgcgtggg gcgattttga ataaaggact ttctgggtcc
     3421 gggcatccat gaccacaaac gacctggccg aaagaggcaa atacgccgct tccgccggcg
     3481 gaaaaggggc cgaaaagaac gatgtgcaaa aagcaagtat gataaaaata gaaatccgtt
     3541 gtctcatgaa tcgcgataca gttcgggtcg tcatttaaaa accagtaagg ctccctctga
     3601 ccttccccta ccggtctggt ttaaattatc ggggtggggg gatttgaacc cacggcctct
     3661 tgctcccaaa gcaagcgctc tagccacctg agctacaccc cgatgtgatt tcactatgat
     3721 gacttacaac gttttctgca agaacggcct ttaggccccg agtgttcccg cgccctgaat
     3781 aacagtagtc cccagccgtc taaaccactt atcagctatt ttcacccttc aaaatctggc
     3841 tctacaaaaa tcaacaaaat tatatcaagg aatgaccact aacacaccga ctttcctaga
     3901 tcgtcatcct aagccgaatc cacaaccacc cgtcaccctg aaccgaatcc cccaccaccc
     3961 gtcatcctga gccgatttac ggcgaaggat ccctttctca agatgcttcg ctcagcatga
     4021 cggtctctcg tcatcccagg ccacgtcacc aaccacccgt caccctgaac ccaatcccta
     4081 accactcgtc atcctgagcc gatttacggc gaaggatccc tttctcaaga tgcttcgctt
     4141 cgctcagcat gacagtctct cgtcatccca ggccgcgtca ccaaccaccc gtcatcctga
     4201 gccggtttat ggcgaaggat ccctttctca agatgcttcg cttcgctcag catgacagtc
     4261 tctcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcatc ccaggccgcg
     4381 tcaccaacca cccgtcatcc tgagcccggt ttatggcgaa ggatctacgc taatattccg
     4441 gaatgacagc tcaaaatccg ggatttgcag aacagaagcc ataaccgtta tgtgccttcg
     4501 gggaagttta ctccggagat ataacgggct gactagcggg aatatgccgt tttccgcata
     4561 ctatgtgatg tgttcttgtt ctgtgatgat ccgggagaaa aacttgtccg tagcaccaaa
     4621 aacaccgaag acaatggacg cgggaattat cgaaacatca gtcaacctga tgagccgtca
     4681 aaaatataga tttttcgagc gcaacaaaaa aatcctgata ggttttagga tcttccactg
     4741 cttttaatcc gaattgagcg ttcgcgcgaa ccaaaagctg tttttcgttt ctttctttga
     4801 cggtgacgga catcttgacc gtggtaatac catgaaattt ttgtccagtg acggttccca
     4861 gatcaagact ggagtcctgg attaaaaaat caagatcctg caacgtattg atgacgcttc
     4921 tcatcattaa cttcttgtcc gtagtgtcga aagcccggct ctggatgctt ctcaaagcga
     4981 cctggctctg gtccgtggac aaaatctgct gttgagacgt agcgcacccg ctggccgaaa
     5041 aaaacaacga cgcaaacaag agaacaagaa aaaaagattt caacctcata ttttttgctc
//
LOCUS       DAOSIO010000124         5052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_14803_length_5052_cov_4.705623, whole genome shotgun sequence.
ACCESSION   DAOSIO010000124 DAOSIO010000000
VERSION     DAOSIO010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14803_length_5052_cov_4.705623"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..435)
                     /locus_tag="PK590_05400"
     CDS             complement(19..435)
                     /locus_tag="PK590_05400"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HOG24065.1"
                     /translation="MEKMIIYQKPGCSICKEVCQILKDSGIEPDSVDYYATPVPKAKL
                     RELAGKMGMPISGLIRDKEQVYRDLRLGEKALTDDQLLDLIAIYPDLLQRPIIERGER
                     AVLARPARKIKAFLASLAPEQAAANTPDLLPPAGES"
     gene            complement(627..1136)
                     /locus_tag="PK590_05405"
     CDS             complement(627..1136)
                     /locus_tag="PK590_05405"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006912253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOG24066.1"
                     /translation="MDILCRGKYLQLVREDNWEYVKRINCRGIVVIVPMTKDKKVVFV
                     EQFRRPLQAQVIEWPAGLVGDHASRASENIETAARRELLEETGYEAKKLTWITEGPVS
                     SGLSSEIITFYAAFDLMKKNEGGGDATEAIKVHEVPLAEAEIWLETMVKNGRLVDPKV
                     YVGLYFLKG"
     gene            complement(1145..2851)
                     /locus_tag="PK590_05410"
     CDS             complement(1145..2851)
                     /locus_tag="PK590_05410"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOG24067.1"
                     /translation="MSRKFSPGSALLGALIYGLVFFNSSAQAASSYKAVVVTPDSRAS
                     QAALKVLEAGGNAADALVTAQAVLNVVAPHSSGIFGGASLLYYDAVLRRVLYFDGSVQ
                     APARASPEMFLDSQQGQPRADYAELRKTGLATGVPGTLKVLSEIQSRFGLRKMSFAEL
                     LEPAAAMAEEGVAVSESLAESLLAHREALQGSGDFQKVFFAGNGLLKAGETVRQEDLA
                     KTFRLLQQKGPAVFYEGELGNAFLDGARQHQPVSGLLDKEDLKSYDLLRTDALYSNYK
                     GYGVFTASPPSAGGVMFLAACHILEKIDLSKYAARPETVHYIGEVMKQVDPLQQWIGD
                     PDHFDLPVVSILSKEWAGKKAANIPASKPGRYENPKHLSLPEGKKPVSVSVLIIDRHG
                     NIANYSASLGDAFGSGRMAPGTGGFLNDLLMDFSMDPGELKSPNFPNAPGPKRRPRNF
                     FMPVVVFKQGKPTLVLNAVNGKESVGAALNAFVQRVDFGVSCGSVLEVPRVFVRDGAL
                     TLEGDLYENDLWRVQLELFGHKIKKENFLGNGQMVCFEEDSGKMVAGSDPRGGEDGAV
                     GA"
     gene            complement(2826..3767)
                     /locus_tag="PK590_05415"
     CDS             complement(2826..3767)
                     /locus_tag="PK590_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HOG24068.1"
                     /translation="MKRVMGKATRDAYGETLVELGGENRDIVVLDADLSKSTKTVLFS
                     EKFPERFFNVGIQEANLAGVAAGLAMCGKIPFASSFASFLVSKGFDQFRMGIAFSEQN
                     VKLVGSHGGITVGEDGASQMSIEDIALMTSLPYFSVIVPSDEISTRALVREMARHRGA
                     VYMRTGRPKAAIVHDVATRFEIGKAVRLRDGKDVTIVANGSEVIESLDAAEALAREGI
                     QAGVIDMHTVKPLDKTLLLEEANKTGRIIVAEEHQIWGGLGSAVAAFLSANHPCKMGF
                     VAIQDTFAESGKPEELLERYGLTTPHIVAVVKKILAR"
     gene            complement(3764..4582)
                     /locus_tag="PK590_05420"
     CDS             complement(3764..4582)
                     /locus_tag="PK590_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOG24069.1"
                     /translation="MNQLSTEELKAKAKIVRRHIVGMTGAAASGHPGGSLSCADILVA
                     LYFNQMNHDPRNPGWADRDRLVLSKGHACPALYSVLAETGYFSPATLTNFRKFGSPLQ
                     GHPDRRKLPGIEASTGSLGQGLSIGIGIALARRLDQKKYFTYVLTSDGEMNEGQTWEA
                     AAMAAHHKVDRLIVFLDYNKFQLDDSTEKICDMEPVVEKWKSFRWHVQEIDGHNMEQI
                     IRATETAKKIQGQPAMIVAHTVKGKGVSFMEGNNHFHGVAPTADEVKRALEELK"
     gene            complement(4743..>5052)
                     /locus_tag="PK590_05425"
     CDS             complement(4743..>5052)
                     /locus_tag="PK590_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24070.1"
                     /translation="AENNLTVDPEFTDPEKGDYTLKNSSPLIDKGNPDAGSYDPFDRL
                     TYPTPNPATTPALPPAKGTIRNDIGAYGGPHAGVIGFVEPTKDDPKTEYIREDVIGTY
                     "
BASE COUNT         1203 a         1440 c         1196 g         1213 t
ORIGIN      
        1 cttaaagctc aacccttctc aagactcacc agccggcggt aacaaatccg gagtatttgc
       61 agcggcctgt tccggagcca aagatgccag gaacgcctta atctttctgg cgggtctcgc
      121 cagaacagcc cgttcacccc gctcgataat cggacgctgg agcaggtccg ggtaaatcgc
      181 gatcagatcc agtaactggt catccgtcag cgccttttcc ccaagtctga gatcccggta
      241 gacctgttct ttgtcccgga ttagacccga aatcggcatc cccatctttc cggcaagttc
      301 ccgcaatttt gctttcggaa cgggggtagc gtaatagtca accgaatccg gctcaattcc
      361 ggaatctttc agaatctgac aaacctcctt gcagatacta cagccgggtt tttgatagat
      421 aatcatcttc tccatttttg atctcctgtt ttacaggcaa aacccttact ttcttaaaaa
      481 cacaaaacct tcgagattaa tcttaaaacc ttggaggccc gttgactgtt tcgggttgtt
      541 gggtttaaga aactttatag cagttagtcc ccaatttctc cgattaacct ggcgtcaagt
      601 cggcgtgttt tgggagacgt ggaaacttag cccttcagaa aataaagccc aacataaact
      661 ttggggtcca cgagtcgccc gtttttcacc atagtttcga gccagatttc ggcctccgca
      721 aggggaactt cgtgcacctt gatcgcctcg gtcgcgtcgc ctcccccctc attttttttc
      781 atgagatcga aggcggcata aaaagtaatg atctcggaac ttaatccact ggaaacgggc
      841 ccttccgtga tccacgtgag ctttttagct tcataaccgg tttcctccag aagttcccgc
      901 cgagccgccg tctcgatatt ttcagacgcg cgagacgcgt gatctcccac aagccccgcg
      961 ggccattcga tcacttgcgc ctgaagaggt cttcgaaact gttcgacaaa aacaactttt
     1021 ttgtctttag tcatgggaac gatgaccaca atcccgcgac agttaattct tttgacgtat
     1081 tcccagttat cttcccgtac aagttgcaga tatttgcccc gacaaagaat gtccatattt
     1141 cacctcatgc cccaacggcg ccgtcttcgc caccccgggg atcgcttccg gctaccattt
     1201 taccagaatc ttcctcaaaa caaaccattt gcccattccc caaaaaattt tcttttttta
     1261 tcttatgccc aaaaagctca agctgaactc tccacagatc attctcatac aaatccccct
     1321 ccagcgtcaa agccccatcc cggacaaaaa ccctcgggac ttccagaaca ctgccacatg
     1381 aaacaccaaa atccacccgc tgaacaaagg cattcagggc ggcccccaca gattccttgc
     1441 cgttgacagc gttcaaaacc agcgttggtt ttccctgttt aaaaaccacc acaggcatga
     1501 aaaaatttct gggccgccgt ttcgggccgg gcgcgttggg aaaattcgga ctttttaatt
     1561 ccccgggatc catggaaaaa tccataagaa ggtcgttcaa aaaacccccg gtccccgggg
     1621 ccatccgccc actgccgaac gcgtcgccca aacttgcgga ataatttgcg atattaccgt
     1681 gccggtcaat gatgagcaca gaaacactca ccggcttttt cccttcggga agagaaagat
     1741 gtttaggatt ctcataacgt ccgggtttac tggccggaat attcgccgcc tttttcccgg
     1801 cccattcttt tgaaaggatt gagacaacgg gaagatcgaa atgatcaggg tctccaatcc
     1861 attgctggag aggatccacc tgtttcatga cttccccgat atagtgtacc gtttcgggtc
     1921 tcgccgcgta ttttgaaagg tcgatttttt caagaatgtg acaggcggcc aaaaacataa
     1981 ccccgcccgc cgatggcggg ctcgccgtaa aaacaccata tcctttgtaa ttcgaataaa
     2041 gcgcgtccgt ccttaaaaga tcataacttt taaggtcttc tttatcgaga agtccggaaa
     2101 ccggctgatg ctgacgcgct ccgtcaagaa aagcgttccc taattctccc tcataaaaaa
     2161 ccgcggggcc cttttgttgt aaaagccgga atgtcttcgc gagatcctct tggcgaaccg
     2221 tctcgcccgc tttcaaaagc ccgttcccgg caaaaaaaac tttctgaaaa tcacccgatc
     2281 cctgaagcgc ttccctgtgg gccagcaacg attcagccag cgattcggaa acggcaaccc
     2341 cttcttcggc cattgccgcc gcgggttcaa gaagttcggc aaaagacatt tttcgaagtc
     2401 cgaaccggga ctgaatctcg gaaaggactt ttaacgttcc ggggacgccg gtcgcgagac
     2461 cagtttttct gagttccgcg tagtcggctc tgggttgacc ctgttgcgaa tccaaaaaca
     2521 tttctgggga tgcccgagcg ggggcctgga cgcttccgtc aaaatagagg acccgtcgta
     2581 aaacggcatc ataataaagc agggacgctc ctccgaatat gccgctggaa tgtggcgcga
     2641 ccacgttcaa gaccgcctgg gccgtcacaa gggcgtcagc ggcattaccg cccgcctcca
     2701 aaaccttgag cgcggcctgc gaagccctgg aatccggggt cacgaccacg gctttgtaac
     2761 tgctggcggc ctgggctgaa gaattaaaaa agaccagtcc gtagataagg gcgcctaaaa
     2821 gcgcgctacc gggcgagaat tttcttgaca acggcaacaa tgtgaggggt cgtcaggccg
     2881 tatcgttcaa gcagttcttc gggtttcccg gactcggcga aagtgtcctg gatggccaca
     2941 aaccccatct tgcaaggatg gttggcggaa agaaaggccg ccacggcgct tccgagtcca
     3001 ccccaaatct ggtgttcttc tgctacgatg atccgaccgg ttttgttagc ttcttcgagt
     3061 aagagagttt tatcgagcgg cttcacggtg tgcatatcga tcactcccgc ctggatacct
     3121 tcccgggcaa gggcctcggc cgcatcaagt gattcaatga cttcactccc gttggccaca
     3181 atcgtaacgt ctttcccatc ccggagccgg acagctttcc cgatctcaaa acgggtggcc
     3241 acgtcatgga caatcgccgc tttcggacga ccagtcctca tgtaaaccgc gccccggtgt
     3301 ctggccattt ccctgaccag ggcccgggtt gaaatttcgt ccgagggaac gatcaccgaa
     3361 aaatagggaa ggctggtcat gagagcgata tcttctatcg acatctggga cgcgccgtct
     3421 tcacccaccg taatgccgcc gtgcgaaccc accagcttca cattctgttc ggaaaacgcg
     3481 atccccatcc ggaactggtc aaatcccttg gaaactagaa aggaggcgaa gctcgacgcg
     3541 aacggaattt tcccgcacat cgcaagcccc gccgcgactc ccgcgagatt ggcttcctgg
     3601 ataccaacat taaaaaaacg ctcgggaaat ttttcagaaa aaagaacagt cttcgtggat
     3661 ttcgacaggt ccgcgtcaag caccacaata tcgcggtttt ctccgcccaa ctcgacaaga
     3721 gtctccccgt aagcgtcacg cgtggccttt cccatgaccc gtttcatttc agctcctcca
     3781 gcgcccgctt tacttcatcc gcggtggggg caactccgtg aaaatggttg ttcccttcca
     3841 taaagctcac gccctttccc ttcacggtat gggccacaat catggccggc tggccctgga
     3901 tctttttagc cgtctcggtc gccctgataa tctgctccat attatgaccg tctatctcct
     3961 gaacgtgcca gcggaatgat ttccattttt caaccaccgg ttccatgtca cagatttttt
     4021 ctgtggaatc atccagctga aatttattgt aatccaggaa aacaatcagg cggtccactt
     4081 tgtggtgggc ggccatcgcc gcggcttccc aagtctgtcc ctcgttcatc tcaccatcac
     4141 tggtcaggac atatgtgaag tatttttttt ggtcgagccg ccgcgcgagc gcgatgccaa
     4201 tcccgatcga aagcccctgc ccgagtgacc cggtcgaggc ctcaattccc ggaagtttgc
     4261 gtcgatccgg atgtccctgg agcggacttc cgaattttcg aaaattggtg agggtggcgg
     4321 gactaaaata tccggtttcc gccaggaccg agtaaagcgc cggacacgcg tgccctttgc
     4381 tcaggaccaa acggtcccga tcggcccagc cggggttcct gggatcatga ttcatctggt
     4441 taaaatagag agcgacaaga atatcagcgc aggagagaga tccgccagga tgtcctgatg
     4501 ccgcggcccc cgtcatcccc acaatgtgac ggcggacaat tttggcctta gccttgagtt
     4561 cttcagtgga aagttgattc atcagttaaa actgtcccta aaagtaatcg atctggtggg
     4621 ctgaaggccg tatcgtatcc acttttttac catgcgtcaa tgtcaaaaaa agtaaaccgc
     4681 tttaaaacaa ctcaattggc cttcagattt gaaaagaaat gaaaaaacga agtgaaaaaa
     4741 tatcagtaag tcccgatcac gtcttcgcgg atgtattcag ttttgggatc gtctttggtt
     4801 ggctcgacaa aaccgatcac tccggcgtga gggccgccgt aggcgccgat atcgtttcgg
     4861 atggtgcctt tcgccggcgg aagcgcgggt gtggttgccg gattcggcgt aggataggtc
     4921 aacctgtcga aaggatcgta agaccctgca tcaggatttc ccttgtcaat cagaggtgac
     4981 gaatttttaa gcgtgtaatc ccccttctca ggatctgtaa attccggatc gaccgtcaaa
     5041 ttattctcgg ct
//
LOCUS       DAOSIO010000125         4990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_15029_length_4990_cov_4.508612, whole genome shotgun sequence.
ACCESSION   DAOSIO010000125 DAOSIO010000000
VERSION     DAOSIO010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15029_length_4990_cov_4.508612"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..975
                     /locus_tag="PK590_05430"
     CDS             136..975
                     /locus_tag="PK590_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24071.1"
                     /translation="MGTRLGIFFAFILSLTAAGGAYYLYQHLTAERSIRIDLEKKYDT
                     TRERVLNLQSEKEQIKAAKEQFEAESEDYRRKAQEYESQVSRLKNETSRIISARTDLE
                     KQLQDKNSQVAEMEKKIVELQQKAEEAMRACNVTPADIKETLSQEEVLSSSTTNEGGL
                     VFTSPGVTPARAPMAVMAVPQSEAVSPGISTVSTPTPPVPLDMTRGSKVLTVNRKFNF
                     VVVNLGLKDGLKMGDSLKVIKSGRVSATLQVEKLYDKFAAATIVTEDQQIPVMEGDEV
                     SKG"
     gene            956..1195
                     /locus_tag="PK590_05435"
     CDS             956..1195
                     /locus_tag="PK590_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24072.1"
                     /translation="MKFPKGSRFYLLVGLILGAICQVAVAQEEPWTAQFLREFEQIKQ
                     RLTQVENQRQEILTRQDKIIEELDMLRIQIRRRSK"
     gene            1211..1432
                     /locus_tag="PK590_05440"
     CDS             1211..1432
                     /locus_tag="PK590_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24073.1"
                     /translation="MRSIRFCFLFMLGLWVGAGCLQAAEEPWTEQFMREFRQIKKQIE
                     NLEKQQAEILEKEEKILEEIARVRIWARR"
     gene            1445..2740
                     /gene="aroA"
                     /locus_tag="PK590_05445"
     CDS             1445..2740
                     /gene="aroA"
                     /locus_tag="PK590_05445"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005369202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOG24074.1"
                     /translation="MTSLTFKGPGQFLGALVPPGDKSISHRALLFGALSEGLSVYENF
                     LESEDCLCLLNVLRMMGVEIEKQPGSTTVSVRGVGLGGLKPPSKELYLGNSGTAMRLL
                     LGVLAGQHFEASLTGDPSLSSRPMRRVTDPLKQMGAQIKGKENANFAPLTIRGGNLRG
                     IDFNNRLASAQVKSALLFAGMFAEGETKILEPVASRDHTERFLRASGAKFKQDGPLLV
                     IEKSDRLSPFQTKIPGDISSAAFFITGAAMTEGSNVLVKDVGLNPTRTGILQVLKRMG
                     AEIEVTVTQEIPEPMGEIRVKGGRLHRTEIAREEIPALVDELPVLMVAMSLAEGESLI
                     SGAEELRVKETDRIRSMVHNLKRVGASIEELPDGCLVKGVSELQGGVVQSFGDHRTAM
                     SLAVASLRSRGEIVVEGTDCIATSYPVFEQHFSHLLKLR"
     gene            2863..3387
                     /locus_tag="PK590_05450"
     CDS             2863..3387
                     /locus_tag="PK590_05450"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOG24075.1"
                     /translation="MAKKNTELQEKILDVDASMQGTISFKDPVNLRINGSFEGKLDTR
                     GNLTIGENAKVTANINGDNITIAGKVTGDIVATKSLSVLGPATIRGNLRTPRLSVTDG
                     AVLEGNISMSGAFEGSEVYLTLKDVAQYLEVEAKVIEEWAQSRKIPGRFENGTWVFSK
                     NEVDAWIQQERARV"
     gene            3458..3766
                     /locus_tag="PK590_05455"
     CDS             3458..3766
                     /locus_tag="PK590_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG24076.1"
                     /translation="MLTLDEVKKYLEIEQDELERLIKENMLHAYKIGGVYLRFRKEEV
                     LNLKYDVLLKAKKSGMQMSFAHKILDFWRFNNFYILSFFVILALFYWFLRSSSQSLPF
                     "
     gene            3784..4167
                     /gene="acpS"
                     /locus_tag="PK590_05460"
     CDS             3784..4167
                     /gene="acpS"
                     /locus_tag="PK590_05460"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOG24077.1"
                     /translation="MKILQGVDLVKISRMEAAIKRGGKRFLERVFSKAERKYCSGKRM
                     MFEHYAARFAAKEALIKATGIRLKKGQRLAEIEVRHEKTGKPFLSLTTAMRRAARLDA
                     RDRVELSIAHEREYAIANVVIVKPR"
     gene            4176..4958
                     /locus_tag="PK590_05465"
     CDS             4176..4958
                     /locus_tag="PK590_05465"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HOG24078.1"
                     /translation="MPSKLLIINADDCNLTPKVTEAILSAHETGIVTSTTFMVNLPIS
                     ESLVRDILDAKKLGLGLHLNITLGEPVAKKALVKGLIQKNGQFRKQKEYFEKGFPKVG
                     EITEEFRAQLQRFVKTFKRLPTHVDVHHHMHDHLPFLEALYAIARKHALPVRWTRLLS
                     EPEIKDRFKPIKTTDHFYGNLDPHTYWTEEVLRHLLFTLLPGTSEVMCHPGWVDDELC
                     AISSLTLPREKEFRLFGSRELRKYIAALGIKLTDFSCLGKKR"
BASE COUNT         1293 a         1085 c         1393 g         1219 t
ORIGIN      
        1 cagatgtttt tcttttggcg gtgctgtcgt gattttctca gccacgcgga tcgtgttttg
       61 ttaagtgagt gttaacccag cgcttcaggt tttacgtatc tagtagcggg aaccacaggg
      121 aggactcata atcgtatggg aacaagatta ggaatttttt tcgcgtttat tctttcgctt
      181 accgcggcgg gtggcgccta ctatctttat cagcatttaa ctgccgagcg tagtatccgg
      241 attgatcttg aaaaaaaata tgataccacc cgagaaaggg ttcttaatct ccagtctgaa
      301 aaggagcaaa tcaaggcggc caaagaacaa tttgaggccg aaagcgagga ctatcgccgg
      361 aaagcacagg agtatgaatc tcaggtgagt cgcttgaaaa atgagacgtc caggattatt
      421 tcagccagga cggatcttga aaaacaactc caggataaaa acagccaagt cgctgaaatg
      481 gaaaagaaaa tcgttgaact tcagcaaaaa gcggaagagg caatgcgcgc ctgcaacgtg
      541 acccctgccg acatcaaaga gactctttcc caggaagagg ttttaagttc ttcgacgacg
      601 aatgaaggag ggctggtctt tacctcgccg ggagtaaccc ctgcgcgtgc gccgatggcg
      661 gtgatggctg tcccgcaaag cgaagccgtg tctccgggaa tttccacggt ttcgaccccg
      721 actcctccgg ttcctcttga tatgacgcga ggctcaaagg tacttacggt taaccggaag
      781 tttaattttg tggttgtcaa tctggggctc aaggacggcc tgaagatggg ggattcgctc
      841 aaagttatta aatcgggaag ggtgagcgcc accctccaag ttgaaaagtt gtacgataag
      901 tttgcggccg cgacgatcgt caccgaagat cagcaaattc cggtgatgga aggtgatgaa
      961 gtttccaaag ggtagccgtt tttacttact ggtcggactt attttgggcg cgatatgtca
     1021 ggtggcggta gcgcaagaag aaccgtggac ggcgcagttt ttacgggaat ttgaacaaat
     1081 taagcagcgt cttactcaag ttgaaaatca gcgccaggaa attctcaccc ggcaggacaa
     1141 aattatcgag gagctcgaca tgctccgaat ccaaattcgc cgccggtcaa aatagaaggg
     1201 tatgcgtgac atgaggtcca tccgattttg tttcctgttt atgttaggcc tttgggtggg
     1261 agcaggttgc ttgcaggccg ccgaagagcc ctggacggaa cagtttatgc gggaatttcg
     1321 ccaaattaaa aaacaaattg aaaaccttga aaaacagcaa gccgaaattt tggaaaagga
     1381 agaaaaaatt ctcgaagaaa tcgcgcgtgt tcgaatttgg gcgcgccgat agatcggttt
     1441 ttcgatgaca tccctcacgt ttaaaggtcc gggacaattt ttaggcgccc tcgtccctcc
     1501 cggcgacaaa tcaatctctc accgcgcgtt gctttttgga gccctctcgg aaggtttgag
     1561 tgtgtacgaa aactttctgg aaagtgagga ctgtctttgt cttctgaatg ttctccggat
     1621 gatgggggtc gaaattgaaa aacagcccgg gagtactacg gtgtcagtcc ggggggtcgg
     1681 attgggaggg ctgaagccac cttcgaagga actgtatctc ggaaattcag ggaccgcgat
     1741 gagacttctt ctgggagttt tggcgggaca acatttcgag gcatcgctga ccggggatcc
     1801 atctttatcg tcccgcccga tgcggcgcgt gacggatccg ctgaaacaaa tgggggcgca
     1861 aatcaagggc aaagaaaacg cgaatttcgc gcctctgacg atccgcggcg gaaaccttag
     1921 ggggattgat ttcaataaca ggcttgcgag cgcgcaggtc aaatccgcgc ttttgtttgc
     1981 cggaatgttt gccgagggcg aaacgaaaat tcttgaaccg gtcgcgtcgc gcgatcacac
     2041 ggagcggttt ctccgggcct cgggcgcgaa atttaaacag gatggccctc ttcttgtgat
     2101 tgaaaagtcg gaccggttaa gcccatttca gacaaaaatt ccgggagata tctcgtccgc
     2161 cgcttttttt attactggcg ccgcgatgac ggaaggttca aatgttctcg tgaaagatgt
     2221 tgggttaaat ccaacgagga ccggtatttt gcaggtgctt aaacggatgg gggcggaaat
     2281 tgaggttacg gtgacccagg aaattccgga gccgatggga gagattagag tgaaaggggg
     2341 acgacttcac aggaccgaaa tcgcccggga ggagataccc gccctggttg atgagttgcc
     2401 cgtgctgatg gtcgcgatgt cgctggcgga aggtgaaagc ctgatctcgg gagcggaaga
     2461 gcttcgcgtc aaggagacag atcggatccg ctcgatggtt cataatttga aaagggttgg
     2521 ggcttcgatt gaagaattgc cggatggatg tctcgtgaag ggggtttcgg agctccaggg
     2581 cggggttgtc cagagtttcg gggatcatcg caccgcgatg agcctcgcgg tggccagcct
     2641 ccgttccagg ggagaaatcg tggtcgaggg tacggactgt attgccacat cataccccgt
     2701 ttttgaacag catttttctc accttcttaa gctccgctaa ttccaggacg taacttgaaa
     2761 cctgggggcc gattcattga caagggtttg gtaaatagac ataattaacc caacgatcat
     2821 accgaaccgt ccgttcgggc ggcaacgaag gaggccaatg ccatggcaaa aaagaatacg
     2881 gaacttcagg aaaagatcct cgatgttgac gcaagtatgc aaggtaccat ctcgttcaaa
     2941 gaccccgtaa accttcgaat taatggcagt tttgaaggaa agctggacac tcgtggcaat
     3001 ttgactattg gcgaaaatgc gaaggtcacc gccaatatca acggggacaa tattacgatc
     3061 gccgggaagg tgacaggcga cattgtcgcg accaagagcc tttctgtttt gggccccgcg
     3121 acaattcgag ggaatcttcg cacgccccgg ttgagtgtga ccgatggagc ggtgctggaa
     3181 ggcaatatca gtatgtccgg ggcttttgaa ggctccgaag tttatcttac cttaaaagat
     3241 gtcgcgcaat acctcgaagt tgaggccaag gtgattgagg agtgggcgca gtcccggaag
     3301 atccccgggc gctttgaaaa cggaacctgg gttttttcga aaaacgaagt tgatgcctgg
     3361 attcagcagg aaagagcacg ggtttgattt tatccacgat ctaaaacgaa ttcctgcttc
     3421 aggttagcgg ggaaatggtt gtgattccat gagcaacatg cttacgcttg atgaagttaa
     3481 aaagtatctt gaaattgaac aggatgaatt ggagcggctg atcaaggaaa atatgttgca
     3541 tgcctacaag attggcggtg tttatcttcg tttcaggaag gaagaagtgc tcaatttgaa
     3601 atatgacgtt cttcttaaag cgaagaaatc aggcatgcag atgtcttttg cgcacaagat
     3661 ccttgatttc tggcggttta acaattttta catcctttct ttcttcgtga ttttggcgtt
     3721 attttactgg ttcctccgct cgtcgtcgca atcgttgcca ttttgacatg acaggattcg
     3781 aaattgaaaa ttcttcaggg agtcgatctc gttaaaattt caaggatgga agccgccatt
     3841 aaacgagggg gaaagcgttt tctcgaacgt gttttcagca aggcggagcg aaaatactgt
     3901 tccggaaaac gcatgatgtt cgagcactat gcggcgcggt ttgcggcgaa agaagctttg
     3961 attaaagcaa ccggtatccg gcttaaaaaa ggccagcgtc tcgccgagat tgaggtgcgc
     4021 catgaaaaaa ccggaaaacc ttttttaagt ttgaccaccg ccatgagacg ggccgctcgt
     4081 cttgatgcca gggatcgggt tgagttgtcc atcgctcatg aaagggaata cgccattgcg
     4141 aacgttgtga ttgtgaaacc gcgatgaaag aaaccatgcc atcaaaattg ctcattatca
     4201 atgccgatga ctgtaatctt acgcccaagg tgacggaagc cattttaagt gcgcacgaga
     4261 ccggaatcgt gaccagcacg actttcatgg tcaatctccc tatttccgag agtttggttc
     4321 gcgatattct tgatgcaaaa aaactgggac tgggactcca tctgaatatt acattggggg
     4381 aacctgtcgc aaaaaaggcg ctggtgaagg ggcttattca aaagaacgga cagtttcgta
     4441 agcagaagga atattttgaa aaaggttttc cgaaagtggg ggaaatcacc gaggagttca
     4501 gggcgcagct tcagcggttc gtgaagacat tcaagcgtct tccgacacac gtcgatgtcc
     4561 atcatcacat gcacgatcac cttccgtttc tggaagctct ttatgcgatt gccagaaaac
     4621 atgcgcttcc ggtgcgatgg acgcgccttt tgtcggagcc ggaaatcaaa gatcggttta
     4681 aaccgatcaa gaccacggat catttctacg gaaatctgga tccgcatact tattggactg
     4741 aagaggtgct gaggcatctt ttgttcacgt tactgccggg aaccagtgag gttatgtgtc
     4801 atcctggatg ggtggatgat gagttgtgcg cgatcagctc gctgacgctt cctcgtgaaa
     4861 aggaattccg gttgtttggt tcgcgcgaac ttcggaaata tatcgccgcg cttgggatca
     4921 aactgacgga ttttagctgt ctcgggaaaa aacggtaggg aagacgttac ttaacgatgg
     4981 tcccgtcaag
//
LOCUS       DAOSIO010000126         4969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_15116_length_4969_cov_3.465201, whole genome shotgun sequence.
ACCESSION   DAOSIO010000126 DAOSIO010000000
VERSION     DAOSIO010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15116_length_4969_cov_3.465201"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PK590_05470"
     CDS             complement(<1..923)
                     /locus_tag="PK590_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HOG24079.1"
                     /translation="MKNLFAVITILSLVISPAVPVFAAEIATTGNQSSDFGGTGIWVQ
                     GLAGQANQRENKDYKAFQDNLMDYTIGFDKIMSPHVRLGGSFGYGYSNKNYKQNRTGF
                     GSYGINDHAFTTVLTATYDSIDLCESREKGKYSPEAVRNQGIDGWYVDSALGFTQHNY
                     DLRTERFDFSPVMEVGKSDYHGQEYTVGMEAGYIFNFGEENLTHVTPYTSLDYGYLYR
                     NLSKAKGFGVNDYKVKGEGATDLEQGFGVQLARSVVSEKLGTFIPYVKGEWLVAYIRD
                     IQSQNRQRNSAAFGAGVIFLNKGHMTCSAGW"
     gene            complement(1295..2635)
                     /gene="yvcK"
                     /locus_tag="PK590_05475"
     CDS             complement(1295..2635)
                     /gene="yvcK"
                     /locus_tag="PK590_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HOG24080.1"
                     /translation="MAQHKILFVGVRRDLAEFNEIFLKAGILFYQITPGAPLPRTERR
                     SRMIVIDLDFLAGRYSEKKIQAYLKRLCQEQYRVICIATFRGYKTLREYHKLGVNDHI
                     LKPFSPRELVLRVRAMIERRKRVACLGGGTGLFTLLTALKTIPGILLTSIVNMSDDGG
                     SSGRLRSSFGVLPPGDIRRSLVALSNAPAVMNELMRYRFVRGQELEDHSVGNLLLTAL
                     SEIKGSMAEAVRSLGDILNVQGVVLPISKNSTRIVAEFEDGTVIRGESLISEGKGRVP
                     SLKIRKIWHEPEAVCEPNAYASLLHADLILIGPGDLYTSVITNLVIPEVQKALVKTPA
                     KKAYLCNLMTKPGETAHFDAAEHVRSLVVSFGRDILDYVIVSNTRFSQSAVARYAKLG
                     QRPVLAGRQRAFREITRAKVILADIGHDTILVRHDESKLRRHIIKLLAQISRAG"
     gene            complement(2904..2977)
                     /locus_tag="PK590_05480"
     tRNA            complement(2904..2977)
                     /locus_tag="PK590_05480"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2941..2943),aa:Arg,seq:acg)
     gene            complement(3196..3798)
                     /locus_tag="PK590_05485"
     CDS             complement(3196..3798)
                     /locus_tag="PK590_05485"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOG24081.1"
                     /translation="MKKTIILAVICFMAVSSGCPLACFAEAQKIDPEGLIPSRKIQPG
                     DTIKINVWKYSDLNATIVVSSAGTMSYSYIGEVQVAGKTPEELRDLITRQLDQNYIAN
                     PRVDVTIESELPTVYVVGEVVRPGSYKYEINLDPLKAVALAGGLSNFASRTALIIRRD
                     PNGIETQIKVKLNELMKANEERAKYQLLPGDMVVVKRGWF"
     gene            complement(3800..>4969)
                     /locus_tag="PK590_05490"
     CDS             complement(3800..>4969)
                     /locus_tag="PK590_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24082.1"
                     /translation="YGFSSEKSPKDGAFVWHPDVTIAYHRPGVLDLSGAYRTNRVFPM
                     GNRYLHFGDGLRHQNASWGVGYFPQKRYAVGYQGSINYYTSKELTQDAVARFRRKPHD
                     AKTARHVVGLNYQLTRRLMAGMSLGMVRTRSRNEGKPGDKSRDREGYLAGSLNYALTR
                     RSNVSLEYSLRHIFEDKDDSLPRSTLRNDIITKTTDFHSFNAGMDYQLTKKISLRGSS
                     QLGIIGKDIYKFGGNFGMTYQLTKKDRISFYYSNIVLQDNMMKVLGWRPGIARGVAGT
                     HSIQIYRAQSISAGYSRQFRIGSAQNSPYTLQINGMYFKREPLSGVYGDWDSIDDGIS
                     FETSIRKPVTEAMSLQIGYRFTNFHSKGADPDSTLDTSTKKHFVFLTIINQFGGS"
BASE COUNT         1250 a         1271 c         1127 g         1321 t
ORIGIN      
        1 tcccagccgg cggaacaggt catgtgcccc ttattgagga agatgacgcc tgcgccaaaa
       61 gcagcgctgt tcctctgacg attctgactt tgaatgtcgc ggatataagc gaccagccat
      121 tcacccttga cataagggat gaaggtcccc aatttttcag aaacaacgga ccttgccaac
      181 tgaacaccaa acccctgctc aaggtcggtc gcgccctccc ccttcacttt gtaatcattg
      241 acgccaaaac ctttagcctt agacaagttt ctgtaaaggt agccatagtc cagcgaggtg
      301 taaggcgtga cgtgggtcag attctcttct ccaaagttaa aaatgtaccc tgcctccatc
      361 cccaccgtat attcctgccc gtgatagtcc gattttccaa cttccatcac cggtgaaaaa
      421 tcaaacctct ccgtgcgaag atcataattg tgttgggtaa atccaagagc gctgtcaaca
      481 taccatccgt caatgccctg gttgcgaacg gcttccgggg aatattttcc tttttccctg
      541 gattcgcaaa gatcgatcga atcgtacgtg gccgtcaaca ccgttgtaaa cgcgtgatcg
      601 ttgattccat agcttccaaa accggttctg ttttgtttgt aatttttatt ggagtagcca
      661 tatccaaaac tgcccccgag ccgtacatgg ggagacatga tcttgtcaaa cccaattgtg
      721 taatccatca ggttgtcctg aaaagcttta taatccttgt tctctctctg atttgcctgt
      781 cccgcgaggc cctgaaccca gatacctgtt cctccaaaat cactggattg gttcccggtc
      841 gtcgcgattt cggcggcaaa cacaggaacg gcgggtgaaa taacgagaga aagaatcgtg
      901 ataaccgcaa acaaattctt catggtgtca tctccttggt tagacgcact tgattgaccg
      961 ctactttctt gcaggtcacg tagctagccg aacaagatct ttaagttctg cctcttaaag
     1021 gcgactcatc taaaacgagt cgttcgagac tccttaagga aaaagcggcg gattatagaa
     1081 tccctattca gaagaatcaa cctctttcct cttcgcaaaa aaccttccgc cttgaagccc
     1141 ttggtgttct tcgtcctttt tggaaaaatc attagccttt tattcagacc ggaattcttg
     1201 cgcgaacgat gggtcaggga agatctctca gatttgcttt acgaaggaaa gaaacaagac
     1261 gcgggcatgg tatataaatt tttccggatc ttttctaccc cgcgcgacta atctgtgcca
     1321 gtaatttaat aatatgtcgg cgtaatttgc tttcatcgtg gcgcaccagg atcgtgtcgt
     1381 gtccgatgtc ggcgagaatt actttagcgc gggttatttc tctaaaggcc ctttgtcgtc
     1441 cggcaagaac tgggcgctgt ccaagtttgg cgtaacgcgc gaccgcggat tgcgaaaacc
     1501 gggtgttaga aacaatcaca taatccagta tgtcccgccc gaaggaaaca accagcgagc
     1561 gcacatgttc ggccgcgtca aaatgagcgg tttctccggg tttggtcata aggttacaaa
     1621 ggtaggcctt tttggccggc gttttaacga gggctttttg aacttcagga ataacaagat
     1681 tggtgataac gctcgtgtaa agatccccgg gaccgatcag gatcaggtcc gcatgaagga
     1741 gtgaagcata ggcgttgggt tcacagacgg cttcgggttc atgccagatt tttcggatct
     1801 tcagtgaagg aacgcgacct ttcccctcgc tgataaggct ttctccccta atcacggttc
     1861 cgtcctcaaa ttcggccaca atacgagtcg agtttttcga tatgggaagc acaacgcctt
     1921 gcacgttcag gatgtccccc agcgagcgca ccgcttcagc catggatccc ttgatctcgg
     1981 aaagggccgt cagaagaaga ttgccgacac tgtgatcttc cagttcctgc ccacggacaa
     2041 accggtaacg catgagctcg ttcatcacag ctggcgcgtt ggagagcgcg acgaggcttc
     2101 ggcgaatatc tccggggggc agaacaccaa acgaagaccg aagtcgtccg gaggaacctc
     2161 catcatcact catgttgaca atggaggtta aaagaatgcc gggaatagtc ttgagggcgg
     2221 tcagaagcgt gaaaagcccc gtgcctccgc caaggcaggc cacccttttt cttcgttcaa
     2281 tcatggcacg aaccctcagc accagctcgc gcgggctgaa cggtttgagt atgtgatcgt
     2341 tcactccgag cttgtgatat tcccgaagcg ttttatagcc ccggaaagtg gcaatgcaga
     2401 tcacacggta ttgttcctgg cacagacgct taagataggc ctggattttt ttttcagaat
     2461 atcttccggc aagaaaatcc aggtcgataa caatcattcg acttctgcgc tccgtccgag
     2521 gaagcggagc tccgggagtg atctgataaa aaagaattcc ggccttcaaa aatatttcat
     2581 tgaactccgc caaatcccgc ctaactccca caaacagaat cttgtgttgt gccatttctt
     2641 tcctcttcaa gagtagtccg tttttctagc gaacttggta cctcattatg tcgttatatc
     2701 ggttgttgtt tatttttatg aagatttttc taatcgtttg atggggaaat ctcgcggaga
     2761 gagcgcatac gtggacaaaa aagacttttc ctctttctac ttgctgctcc ggaacagccg
     2821 tgggaagtaa aaattcccga gcggagacct tacccgacgc acgcaaaaat cgcctgcctt
     2881 ttcaagcggt gtttttctgt gatcgcgccc ggcaggattt gaacctgcaa ccctcagctc
     2941 cgtaggctga tgctctatcc agttgagcta cgggcgcaag caactacgtt tgccaaatta
     3001 aagcctgcga ccagccccga cagtcaatct tcctgtcaag aaagaccgcc ggaaacgggc
     3061 gtattataag tattttctta attattgcaa aagatgcctt aggaattttt ctcagatttt
     3121 gaacaaatta gacccgccag cttgaacaaa tcggacagac cccctttgcg gaggcggtca
     3181 ccataagtca aaaaattaaa accatcctct cttgacaaca accatgtctc ccggaagaag
     3241 ttggtatttg gcccgctcct cattggcctt catcaactca tttaatttaa ccttgatctg
     3301 cgtttcaatc ccattggggt ctctgcgtat gattagagcc gtccgggaag caaaattgct
     3361 cagcccaccg gccaaggcaa cggccttgag agggtccaga ttaatttcat atttgtagct
     3421 acctggtctt acaacttccc cgaccacata aaccgtgggc aattctgatt cgatggtaac
     3481 gtctactctt ggatttgcga tataattctg atcaagctgg cgggtgatga gatcccttaa
     3541 ctcttccggc gtctttccgg caacctgcac ttccccgatg taagaatagc tcatggttcc
     3601 cgccgaactc accactatgg tggcgttaag atcgctatac ttccaaacat tgatcttgat
     3661 agtgtctccg ggctgtatct tgcgggacgg aattaaccct tccggatcga tcttctgcgc
     3721 ctccgcaaaa caggccaaag gacagccact actcacagcc ataaaacaaa tcactgccag
     3781 tattattgtt tttttcatgt caggatcctc caaattgatt gatgatggtc agaaaaacaa
     3841 aatgcttttt tgtggatgtg tcgagcgttg agtctggatc cgctcctttg gaatgaaaat
     3901 ttgtaaaacg gtatccgatt tgtagagaca ttgcttccgt gaccggtttt cggattgagg
     3961 tttcaaagga gatcccgtcg tctattgaat cccagtctcc atacactcca ctcaaaggct
     4021 cccttttaaa gtacatcccg ttaatttgca atgtataagg cgagttttgg gctgaaccaa
     4081 tgcgaaactg gcgcgagtat cccgcggaga tgctttgagc cctgtaaatc tggatagagt
     4141 gggttcccgc aactcctctg gcaattccgg gtcgccaccc cagaaccttc atcatattat
     4201 cctgaagcac gatattgcta tagtaaaaag aaatcctgtc ttttttggta agttgatagg
     4261 tcattccgaa attacctccg aacttataga tgtctttacc gataattccc agctgagagc
     4321 taccgcgcag tgaaattttt ttcgtaagct gataatccat gccggcatta aaggaatgaa
     4381 agtctgtggt ttttgttata atgtcgtttc gtagcgtact cctgggaaga gaatcatctt
     4441 tgtcctcaaa aatatgccgc aacgaatatt ctaaagaaac attgctccgg cgggtcaagg
     4501 catagttaag agaacccgct aaatatccct cccggtccct tgatttgtct ccgggtttgc
     4561 cttcatttct cgaacgggtg cgcaccatcc ccaaactcat ccccgccatt aatcttcggg
     4621 tcagttgata atttagccca accacatgcc gggcagtttt ggcatcatgc ggctttctgc
     4681 ggaaccttgc cacggcatct tgagtaagct ctttgctggt atagtaattg atcgacccct
     4741 ggtatcccac ggcatacctt ttttgaggaa aataaccaac accccaggat gcgttttgat
     4801 gtcgcaaacc atcgccaaaa tggaggtacc gattccccat agggaaaaca cgattcgtcc
     4861 gatacgctcc cgagagatcc agcaccccag ggcgatgata ggctatggta acatcggggt
     4921 gccagacaaa ggctccatct ttgggacttt tttcactgct aaatccata
//
LOCUS       DAOSIO010000127         4953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_15175_length_4953_cov_3.880768, whole genome shotgun sequence.
ACCESSION   DAOSIO010000127 DAOSIO010000000
VERSION     DAOSIO010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15175_length_4953_cov_3.880768"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1083)
                     /locus_tag="PK590_05495"
     CDS             complement(136..1083)
                     /locus_tag="PK590_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG24083.1"
                     /translation="MKQKIVASRVFWGMVCGALLLLFSSSCGVAADDVTQNELGFSTG
                     DSPVSAEGQDVDKLLYTLNETLQENRKIRQSMRDLQTAFEKVTLEKSDVMGQMRKVEQ
                     LAIQKSNETSRRIKELTDQLGATKKDLETMQADYRNLLDEKNNLEKDLDGLRSDKKKT
                     EELLAQSILTEERDQIVERMKINEEAVEKALMSEATVNRENLDLKAKLIQAHFELGNL
                     FYDLGRYQEAKTQYDYVLQWDPDHAKAHHNLAVIYDYAFHQLPEAKDHYQRYMNLKSP
                     SEEAHEVRMRLWDIAQLTKVLPEEPLKQDFKRYRKMPSA"
     gene            complement(1141..2841)
                     /locus_tag="PK590_05500"
     CDS             complement(1141..2841)
                     /locus_tag="PK590_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOG24084.1"
                     /translation="MRRYQSACFAVLWCLTICAIPGALADTVPLSLPESSGSGMEKSV
                     FLDLRDINVIDVFKFLAIQGNFNIVTSKNVQGRSTLLLRNVKIKDALDILALSNQLAY
                     ETRNDITYIMTEDEYAALHGKNFNDKKKIMIRTLKNAKPSYALAALQGVQSAIGKVII
                     DEDTGSVVMIDIPEKLIQMNSILDQVEKNLETKVIKLQFAKADDVASLLRPQLEGKGV
                     GTVIADLRSNQIMLSAYPDRLDEASRLVQALDKQTRAVLIETRILQLTLNPKFQFGVE
                     WEKKFPVSNFRNLDFKSDFSFPEVTSVPDFGSMRIGEVDRDSFMLSITAMKEIESTRV
                     LAQPKLMILDREEAKLNIGDRIPYVVSTTTATSGSNTGISQDVRFIDVGLMLAVTPII
                     NDDGFVTMKIRPEISSKTGDIKSFVGSVNGADGKPLEVYNLIPIVNSTVVESTVIVRD
                     GISVILGGLIRDEMTESNRGVPYLMDIPLLGNLFKSRGEEIKKTEIVIIITPHIVTGH
                     KEVLNRPIPIIKSNEGQTPGIPGTDNPVHLMTGEVLGEARESAESLDVSRGDSMELMR
                     "
     gene            complement(2852..3541)
                     /locus_tag="PK590_05505"
     CDS             complement(2852..3541)
                     /locus_tag="PK590_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24085.1"
                     /translation="MAESNNPSLTPEKRLLDLIEEPDSLKRETTVRKETQRPKTFWTD
                     LGLQKKWTDLKGRSEVFFASQKKTFGIKTINQVLLVVVSVIVIVLMVSVVVDWAGMGK
                     DFTKSIQIPDSKMLEVEIPQEGIPEDLAAEQWDLGKMFMPYAKRREEADKLQQEQSTR
                     LAELIKKLKLTGISFDPADVAMASCMIEDMEKGITTFLREGDPVGGLTVVKIHEDHVV
                     LGQKNETIEMR"
     gene            complement(3514..4392)
                     /locus_tag="PK590_05510"
     CDS             complement(3514..4392)
                     /locus_tag="PK590_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG24086.1"
                     /translation="MMSYYRILGFEKEPFSTSPDPEFLYLAQGHQSTMTNVLIELRLR
                     RGLSVVLGDVGTGKTTLSRKLIQALRERGDFIVHYVLDPCFANEHEFLLSIARNFSIQ
                     LDPGSAHWQDLNHASVLELKEAVEKFLYEKTVIGNATVVLIIDEAQKLNEASMEMLRV
                     ILNYETNDTKLLQLVLFGQLELHSKITKIPNFLDRISFKYALNPLSLSESREMILFRI
                     KQANYCSDSLLFQDDAIEMIHRISQGYPRKMTMLCHRALKQLVMKRKWVVDEEIIEEI
                     VSEDMRAGWLSPTIHH"
BASE COUNT         1218 a         1360 c         1054 g         1321 t
ORIGIN      
        1 cataaatcaa cattttgtca tgggaaaaca tgcgtgggaa acgcgcgtga ttcgagggga
       61 gatcatctct caaaaatacg tcttgggaag cagtacaaga aggggaaatt cgggagtgtt
      121 taagtaccgt ttccgtcagg cggaaggcat ttttcggtac cgcttaaaat cctgttttaa
      181 cggctcttcg ggcaatactt tggtcagttg agcgatgtcc cagagacgca tcctgacttc
      241 gtgagcctct tcgctcggag atttcaaatt catgtatcgc tggtagtggt ctttcgcctc
      301 cggcaactga tgaaaagcgt aatcgtagat taccgctagg ttgtgatggg ccttcgcgtg
      361 gtccggatcc cattgaagga catagtcata ttgagtcttt gcttcctggt aacggcccag
      421 gtcataaaac agattaccta actcaaaatg cgcctggata agcttggctt tcaaatcaag
      481 gttttccctg ttgaccgtcg cctcactcat cagagctttt tcaactgcct cttcgttgat
      541 cttcatccgc tccacgatct gatcccgttc ctcagtcaaa atcgactgcg ccagcaattc
      601 ttccgtcttt tttttatcgc tacgcaaccc gtcgagatct ttttccagat tgttcttttc
      661 atccagtaaa ttccggtaat ccgcctgcat cgtttctaaa tcttttttgg tcgcgcctaa
      721 ttgatcggtg agttctttaa tcctcctgct cgtctcattg cttttttgaa tcgcgagctg
      781 ctcgaccttt cgcatctgtc ccatcacatc gcttttttca agcgtcactt tttcaaaagc
      841 cgtttgaaga tccctcatac tctgtcgaat tttccggttt tcctgcaacg tctcattgag
      901 cgtgtacaaa agcttatcga catcctgtcc ttccgccgac acaggggaat cccctgtcga
      961 aaaacccagc tcattttgcg tcacatcgtc cgcggccacg ccacaggatg aggagaaaag
     1021 cagaagcaaa gcgccgcaaa ccattcccca aaaaactctg gaagccacga ttttttgttt
     1081 cataacgtta tcctttgcgc ccgcaaaatc ctcttacgaa aaacatcgac ccgccaagct
     1141 tcaacgcatg agctccattg aatccccacg agacacatcc aaactctccg cggactcgcg
     1201 cgcttcaccc aacacctcgc ccgtcatcag gtgtacgggg ttatcggtcc cgggaatccc
     1261 cggcgtttga ccttcattcg atttgataat cgggatcgga cgatttaaga cttccttatg
     1321 accggtaaca atgtgcggcg tgataataat gacaatctcg gtttttttga tttcctcgcc
     1381 acgactcttg aacaaatttc ccagaagcgg aatgtccatc aagtacggta ccccccggtt
     1441 gctttccgtc atttcatccc gaataaggcc gccgagaatc accgaaattc catcgcggac
     1501 aataacggtc gattccacca ccgtgctatt gacgatgggg ataaggttgt aaacttccag
     1561 gggctttcca tccgcaccat tcacgctccc cacaaacgac ttgatgtcgc ctgttttgct
     1621 tgaaatctcc gggcggattt tcatggtaac aaaaccgtca tcattgataa tcggcgtcac
     1681 cgcgagcatc agccccacat cgataaaccg gacatcttga ctgattccgg tattgcttcc
     1741 cgaagtcgcc gtcgtcgtcg aaaccacgta agggatgcgg tccccgatat taagtttcgc
     1801 ctcttcacgg tccagaatca tcagtttggg ctgagctaac acccgggtgc tttcgatttc
     1861 cttcatggcc gtaatcgaca acataaagct gtcgcggtcc acttcgccga ttctcatgct
     1921 tccaaaatcc gggaccgagg tcacctcggg aaatgaaaaa tcacttttaa aatcgaggtt
     1981 tcggaaatta ctcaccggaa actttttctc ccactcgaca ccgaactgaa atttgggatt
     2041 cagggtcaac tgaaggatcc gggtctcaat caataccgcc ctcgtttgtt tgtcgagggc
     2101 ctgcacgaga cgtgaagcct catcaagccg gtcggggtaa gcggaaagca taatctggtt
     2161 ggagcgcaaa tccgcgatca ccgttcccac tcccttgccc tcaagctggg gacggagcaa
     2221 cgacgcgacg tcatccgcct tcgcgaactg aagcttgatc accttggtct caagattttt
     2281 ttcaacctgg tcaagaatac tgttcatttg tatcagcttt tcgggaatat cgatcatcac
     2341 caccgaaccc gtgtcctcgt cgataatcac ctttccgatg gcgctctgga cgccttgcag
     2401 ggcggccaac gcgtagctgg gcttcgcgtt tttaagagtt cgaatcataa tttttttctt
     2461 gtcgttgaaa ttcttgccat gcaaagcggc gtactcatcc tccgtcatga tgtaggtaat
     2521 gtcgttccgg gtctcatagg ccagctggtt ggatagcgcg agaatatcaa gagcatcctt
     2581 gatttttaca ttgcgcaaaa gcaacgtcga tcgcccctgg acgtttttgc tcgtgacgat
     2641 attaaaattt ccctgaatcg cgagaaattt aaaaacgtca atgacgttga tgtcacgaag
     2701 atcgagaaag acggatttct ccattcccga gccggaactc tccggcaagg aaagcggcac
     2761 cgtatccgcc agcgctccgg gaatcgcgca aatcgtcaaa caccacagaa cggcaaaaca
     2821 cgcgctttgg taacggcgca tttggttttt tttaacgcat ctcgatagtt tcattttttt
     2881 gcccaagaac gacatgatcc tcatgaattt ttacgaccgt caaccctccc acgggatccc
     2941 cttcccgcaa aaacgtcgtg attcccttct ccatgtcctc aatcatgcag gacgccatcg
     3001 ctacatcggc cggatcaaag gaaatacccg tcagctttaa tttttttatc aattcagcaa
     3061 gccgtgtcga ctgttcttgc tgaagcttat cggcctcttc ccgacgcttc gcgtaaggca
     3121 taaacatctt gcccaaatcc cactgctcgg ccgccaaatc ctccggaata ccttcctgag
     3181 gaatctcaac ttcaagcatt ttactgtccg ggatctggat ggattttgta aaatcctttc
     3241 ccatccccgc ccaatccacc accacactta ccatcaagac aatcacaatc acactgacca
     3301 cgaccaacag aacctgatta attgtcttga tgccaaacgt ttttttttgc gaagcaaaaa
     3361 aaacttcgct acgtcctttt aggtccgtcc attttttctg caatcccagg tctgtccaga
     3421 aagtcttggg cctttgcgtt tcctttcgaa ccgtcgtttc cctcttcaaa gaatcgggct
     3481 cttcgatcag gtcgagaaga cgcttctccg gcgttagtga tggattgttg gactcagcca
     3541 tcccgcacgc atatcctcgg aaacaatctc ctcgataatc tcctcgtcca ccacccattt
     3601 acgtttcatc accagttgct tgagcgctcg gtgacaaagc atcgtcatct tccggggata
     3661 cccctgtgat atcctgtgga tcatttcaat agcgtcatcc tgaaacagaa gcgaatcgga
     3721 gcaatagttt gcctgtttga tacgaaacag gatcatctcg cgcgattcac tcaggctcaa
     3781 cggattcagc gcgtatttaa aactgatacg atcaagaaaa ttgggtattt tcgtaatctt
     3841 ggagtgaagt tcaagttgtc caaaaaggac cagttgcaga agcttggtgt cattggtttc
     3901 ataattcaaa atcacgcgca acatttccat ggacgcttcg ttcagttttt gcgcctcgtc
     3961 aatgatcaga acgaccgtcg cgttgccaat caccgttttc tcgtacagga acttttcaac
     4021 cgcctctttc agttccagga cgctcgcgtg gtttaaatcc tgccaatgcg cgctccccgg
     4081 gtccagttga atcgaaaaat tccgggcgat cgaaagaaga aattcgtgtt cattcgcgaa
     4141 acaaggatcc aagacataat gaacgataaa atcccctctc tcgcgaagcg cctgtatcaa
     4201 ttttctcgaa agcgtggtct tccccgtccc gacatcgccc agcacgacgg acaatccccg
     4261 ccgcaggcga agctcaatta aaacgttcgt cattgtcgac tggtgtcctt gcgcgagata
     4321 cagaaattcg ggatccggac tggtcgaaaa aggctctttt tcgaagccta atatccggta
     4381 ataactcatc atatcctcct ttgagaatcc attccctgac ctgttttcca agacccggaa
     4441 ataaaaaata cgagatgtcc taaacgcatc cgtatatact tcggcacaac ttctaaaatt
     4501 attaagttag aaatcgtccc gcccaatccg ggtcgatacg cccacaggca ggtttgtggg
     4561 actcaaaaca gctatcagaa tctgcttatt cgcgcgtcat taccggccca aaaatgaatc
     4621 gctaactgat cgggccggaa gacattcccg gcttccctct caccttgaaa ttcaatatgg
     4681 gcgatgatat agttccgcgc ctcaaatcag caaatcacaa aaaccacctg cttgtgtttt
     4741 tccggggccc gattgcccca ggcaaccgac gggcaagaaa aatttaacat gacgcaaaac
     4801 caaaatatta aaaacgagca gtacttaacg gatgtccaac gacttgattt tattgatgcc
     4861 ttgcggggat gggcattttt gctggtgctg atcgggcatg ttaatcagaa attcggcttt
     4921 ccgtccctgg tggccagctg gctggtaccc ggc
//
LOCUS       DAOSIO010000128         4776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_15861_length_4776_cov_4.191061, whole genome shotgun sequence.
ACCESSION   DAOSIO010000128 DAOSIO010000000
VERSION     DAOSIO010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15861_length_4776_cov_4.191061"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PK590_05515"
     CDS             complement(<1..769)
                     /locus_tag="PK590_05515"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24087.1"
                     /translation="MLKLFRINRGRFYQVLIDAGLILFSLVTAYALTEKDGCFTQIHV
                     RRILFAAPFFLGIKLVINAAFRIYRQLWRYIGIRDAVNIFKAVSLSDLALMAFFFSVK
                     GRIFSPVMFALDWVLCLGFFSGVRLLRRYQMMKKIESRRVPVAKKKVLLYGAGSGGEL
                     ILRRIQSEPSIKMMPVGFIDDDPEKMGAVLHGVEVLGGVEKTKEMIGRFHVEEILITS
                     PTMPATRIEQVSQIAKNEQISIKIAPSFYGMFQGGTES"
     gene            898..1254
                     /gene="folB"
                     /locus_tag="PK590_05520"
     CDS             898..1254
                     /gene="folB"
                     /locus_tag="PK590_05520"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOG24088.1"
                     /translation="MDSKIFIRNLEVRCRIGTLPRERTRKQKVVIDLEFPAPVRRAAK
                     TDDLRKALNYQAIADCVTEFVSASRFFLIETLADRLASLLLKKFSLRELSLTVSKPGA
                     LKNARTVGVTLRRNHP"
     gene            complement(1251..1559)
                     /locus_tag="PK590_05525"
     CDS             complement(1251..1559)
                     /locus_tag="PK590_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24089.1"
                     /translation="MNKTVFSIFFAVIVFLFAVVPDAGASDSQNIAYIQRGVGRILGS
                     TLAIPAAMVQDSRCVMFPFGIVTGALRGTVRMVGSIVGGVVDVARGGAPYAKYAALAL
                     "
     gene            1619..2542
                     /gene="rfaD"
                     /locus_tag="PK590_05530"
     CDS             1619..2542
                     /gene="rfaD"
                     /locus_tag="PK590_05530"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HOG24090.1"
                     /translation="MTKHILVTGGSGFIGSNLVMELQNSFPEDFITVLDDFRSSSFKN
                     LLGFRGDILTYNCADKAWMGWAKGRKIRIIYHMASITDTTVHDQQKMMFDNVEGFRNV
                     LDLATGKKVPVVYASSAATYGTIDRPIKESDAGQANNIYGYSKWIMDQTARAYYSKIK
                     VVGLRFFNVFGPGESYKGHTSSMIYQLCLQMMEGKRPRIFKWGEQKRDHIYVKDVVSA
                     LLMAHQSKASTVMNLGTGRATSFNEIVDALNEALGTSLKPDYFDNPYTAFYQNYTQAD
                     MSHHESMTGFRCQYTTREGIIDYVRNYLSKK"
     gene            2581..3684
                     /locus_tag="PK590_05535"
     CDS             2581..3684
                     /locus_tag="PK590_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOG24091.1"
                     /translation="MVHPDGASASPAGLADLMKREHFISSFFFIALIFIVYLVIRIFS
                     PFASAMFWSAILTFALYPAYRPIRERFPEHTTLTAAIMTFLVFILIILPLVVILINVT
                     TQAIETSQWVYDYVRQGKLEQMIESIRATSWIQKIETKIVQWDILKQNLTSWLLAVTK
                     NAGNYTASQVGVLTKNIFFFLLNFFFMFFLTFAFFKHGTAIYEFIYQIAPFEKETKDA
                     VFQQINDTFTAVIRGQILTSFAQSLLAGIIFWVLGIPVPVLFAFFLFLTSLVPILGAT
                     AIWAPLTLYLLLSQLYLKALILAVVGVLGISLLDNIIKPAIIGERTKLPYFLLFFGIM
                     GGLKFFGLMGIFLAPVVLAVFFALIRIYRQQYL"
     gene            complement(3725..>4776)
                     /locus_tag="PK590_05540"
     CDS             complement(3725..>4776)
                     /locus_tag="PK590_05540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOG24092.1"
                     /translation="DVFLKSHSVPRLRLGDRVVEIPSCQPIDAAGMRALAESILTAPQ
                     IQTLQQNKSVDFGFDHSAKGCRFRGNAFFQQGTLSMVFRLLWKGIPRFSELNLPPVLE
                     KVALEKSGIILIAGTVSSGKTTTLATMIQTMNEKVQKHIMTIEDPVEYLHTDGQCLIQ
                     QREVGGDTESFNSAIKYMVRQSPDVVVIGEMRDAESFNFALASAEVGRLVISTVHAQS
                     VYQVFDRVLGFFQPEQRESILRHFAMNVTCIAAQKLLVGADGTSLIPAVEIMLGNYTV
                     RQLILEHKIDKLPQALKNCAQEGMQTMDQSLFQLWEKKLISKETALANSQAPQELEAW
                     MKGIRIGQDTKILGG"
BASE COUNT         1192 a         1318 c         1072 g         1194 t
ORIGIN      
        1 acgactcggt tcccccctga aacatcccgt aaaaagacgg ggcgatttta atgctaattt
       61 gttcgttttt tgctatttgg gagacctgct caattctcgt cgcgggcatc gtgggggagg
      121 taataagaat ttcttccacg tgaaatcttc cgatcatctc ttttgttttt tcaacgcctc
      181 ccaacacctc taccccgtgc aaaacagccc ccatcttctc aggatcatcg tcgatgaaac
      241 ctaccggcat cattttgatc gagggttcgc tctgaattct cctgagaatc aattcccccc
      301 ccgatcccgc gccgtagagc agaacctttt tctttgcgac cggcaccctt cgactttcaa
      361 tttttttcat catctggtaa cgacgcaaaa gtctcacccc cgaaaaaaat ccgaggcaca
      421 aaacccagtc cagcgcgaac atcacgggag aaaaaatcct ccctttgacg gaaaagaaaa
      481 aggccatcag ggcaaggtcg gaaagtgaaa cggccttgaa gatgttgacc gcgtcccgga
      541 tcccgatgta ccgccagagc tgacggtaaa tcctgaacgc agcattgatg actaatttaa
      601 tgcccagaaa aaaaggagcc gcaaaaagga tccgccggac atgaatttga gtaaaacacc
      661 cgtccttttc cgtcagggcg taagccgtta ccaacgaaaa aagaatcaac cccgcgtcaa
      721 ttaaaacctg ataaaacctt cccctgttaa tccggaacag tttcagcacg ctcaaatctc
      781 cctgggcaat acttttaacg tgttagagtg aaccgaaagt ctaatcattc acaatcgcat
      841 ccacttttcc ctcgataaac cttgcaactt tctcttgaaa tgatagatta gcccatcatg
      901 gactcaaaga tttttatccg aaatctggaa gtgcgttgcc ggatcgggac cctcccccgc
      961 gaacgaaccc ggaaacaaaa ggtcgtgatc gacctcgaat ttcccgctcc cgtgcgccgt
     1021 gcggccaaaa cagatgacct ccggaaagct ctaaactacc aggccatcgc ggattgcgtc
     1081 acggaatttg tttcagcaag ccgctttttc ctcattgaaa ccctggcgga ccggctcgcc
     1141 tcattgctcc tgaaaaaatt ttcccttcga gaactttctc tgacggtctc caaacctgga
     1201 gctctcaaaa acgccagaac cgtaggcgtt accctccgga gaaaccatcc ctagagcgcg
     1261 agcgcggcgt acttggcata gggagcaccg ccccgtgcga cgtctaccac cccgccgaca
     1321 atgctcccga ccatccgcac cgttccccgc aacgctcccg tcacaattcc aaacggaaac
     1381 atgacgcacc gggaatcttg gaccatcgcc gccggaatgg ccagggtact cccgagaatc
     1441 ctgccgaccc cgcgctggat gtaagctata ttctgtgagt cggacgcccc cgcgtcggga
     1501 acaacggcaa ataaaaaaac aataaccgca aaaaaaatag agaaaaccgt tttattcata
     1561 ccctgaatat cggcatttaa agattgaact tttgaaaaat ttcgatagca tgtccggcat
     1621 gacaaaacac attctagtga caggcggttc cggttttatc ggttccaatc ttgtcatgga
     1681 gctccaaaac agcttccccg aggatttcat caccgtcctg gacgacttcc ggagctcatc
     1741 cttcaaaaac cttctcggat tcagaggcga catcctcact tataactgcg cggacaaggc
     1801 atggatgggc tgggccaagg ggcgaaaaat aagaattatc taccacatgg cttcgatcac
     1861 ggatacgacg gttcatgatc agcaaaaaat gatgttcgac aatgtggagg gatttcgaaa
     1921 tgttctcgat ctcgcgaccg ggaaaaaggt tccagtagtt tacgcttcct cagccgccac
     1981 ctacggaacg atcgaccgcc cgatcaagga atctgacgcg ggacaggcca ataacattta
     2041 cggctattcc aagtggatca tggatcaaac cgcgcgcgcg tattattcca agattaaagt
     2101 cgtggggctt cgtttcttta acgttttcgg ccccggtgaa agctacaagg gccacacgtc
     2161 aagcatgatt taccagctct gtcttcagat gatggaggga aaacggccgc gtatttttaa
     2221 atggggtgag caaaaacgcg atcacattta tgtcaaagac gtcgtgtccg cgttgctgat
     2281 ggcccatcag tcgaaagcat ccaccgtcat gaaccttgga acgggaagag cgacttcatt
     2341 caatgaaatt gtggatgccc tcaacgaggc gcttgggacc agtctgaaac cggattattt
     2401 tgataatccg tacacggcct tttatcaaaa ttacacgcag gccgacatga gccatcacga
     2461 atcaatgacc ggatttcgct gtcaatacac gacccgtgaa ggcatcattg actacgtccg
     2521 gaattacctt tctaaaaaat aactcggagc cggagagttt aacgatgatc actgtcataa
     2581 atggttcatc ccgacggggc cagcgcatcg ccggcgggac tcgctgacct catgaagcgg
     2641 gaacatttca tttcttcgtt cttttttatc gcgctcattt ttattgttta tcttgtcatt
     2701 cggatctttt caccttttgc ctccgccatg ttctggtccg cgattttgac attcgccctc
     2761 tatcccgcct accgccccat tcgcgaacgt tttccggaac ataccacgct tacggccgcc
     2821 atcatgacgt ttctcgtctt catcctaatc atcctgcccc tggtggtgat cctgattaac
     2881 gtcactaccc aggccatcga aacttctcaa tgggtctatg attacgtccg gcaggggaaa
     2941 ctggagcaga tgatcgagag catccgggcg acaagctgga tccagaagat tgaaacaaag
     3001 atcgtccagt gggatatcct gaagcaaaac ctgacgtcat ggctcctggc tgtcactaaa
     3061 aatgcgggaa actacacggc ctcacaggtc ggggttctca caaaaaacat ctttttcttt
     3121 ctcttgaatt tcttttttat gttctttctc acgttcgctt tcttcaaaca cggcacggct
     3181 atttatgaat tcatttatca aatcgctccg tttgagaaag aaaccaaaga cgccgtgttt
     3241 cagcagatca atgatacgtt cacggcggtc atacgcggac aaattctcac cagctttgcc
     3301 cagtccttgc tggcaggaat tattttttgg gttctgggaa ttccggttcc tgtcctcttt
     3361 gcttttttcc ttttcctgac ctcgcttgtc ccgatcctcg gtgcgacggc aatctgggcg
     3421 ccactcaccc tttatctcct cctctcccag ctctacctga aagccctcat cctggccgtg
     3481 gtgggagttc tcggaatcag tctactggac aacattatca aacccgcgat catcggggaa
     3541 agaaccaagc ttccctattt tctgcttttt ttcgggatta tgggagggtt aaaatttttc
     3601 ggcttgatgg gtatttttct tgccccggtt gtcctcgcgg tcttcttcgc gttgatcaga
     3661 atttaccgac aacaatatct ataaaaaacg gcagtcggcg ggagaccatt cctctcccct
     3721 tttttcatcc ccccagaatt ttggtgtcct ggccgatcct gattcccttc atccaggctt
     3781 ccagttcctg cggagcttga ctattggcaa gggccgtttc ttttgaaatt aacttctttt
     3841 cccagagttg aaacagggat tgatccatgg tctgcatccc ttcctgagcg cagtttttaa
     3901 gcgcctgggg gagcttgtca attttatgtt ccagaataag ctgtctgacc gtgtaattac
     3961 cgagcataat ttctacggcc gggataaggc tcgtcccatc tgcccccacc agaagttttt
     4021 gggccgcgat acaggtcaca ttcatcgcaa aatgccgaag tatggattca cgttgctccg
     4081 gctgaaaaaa tccgagaacg cggtcaaaaa cctggtacac ggactgggcg tgaactgtcg
     4141 aaatgacgag acggcccact tcggccgagg cgagcgcgaa attaaaagat tcggcgtccc
     4201 gcatttcccc gatcacgacg acatcgggag actgccgcac catatacttg atcgccgaat
     4261 taaagctttc cgtgtcgccg ccgacttcgc gctgttgaat caggcattgt ccgtccgtgt
     4321 gaaggtattc gaccggatct tcgatcgtca taatgtgctt ctggactttt tcgttcatgg
     4381 tctgaatcat cgtcgcgaga gtcgtcgttt ttcctgaaga gactgtcccc gcaatcagga
     4441 taatgccgga tttttcaaga gccacttttt caagaaccgg cggaaggttt agttctgaaa
     4501 accggggaat ccctttccag agaagccgga acaccatgga aagtgtcccc tgctgaaaaa
     4561 aagcattgcc gcgaaaccgg caccccttgg ccgagtggtc aaatccgaaa tcgacactct
     4621 tgttttgctg aagtgtttga atctgcggag ccgtcaaaat actttcggca agcgcccgca
     4681 tccccgccgc gtcgatcggc tggcacgatg gaatctcaac gacacgatct cccagcctca
     4741 gccggggaac cgagtgggac tttagaaaaa catcct
//
LOCUS       DAOSIO010000129         4745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_15997_length_4745_cov_3.548401, whole genome shotgun sequence.
ACCESSION   DAOSIO010000129 DAOSIO010000000
VERSION     DAOSIO010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15997_length_4745_cov_3.548401"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1885
                     /locus_tag="PK590_05545"
     CDS             <1..1885
                     /locus_tag="PK590_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24093.1"
                     /translation="WFPVALYLLERYLEGYKSRWLFGLAVVVGQSMVAGYVQMAAFTW
                     IIFAVYAVLRVLFFPDSSGGWIFRIVLLGRLVLSAVGAVGLALPQLFLTYQLAILSNR
                     TGLEEGYAYVGSMAPYILGTLFNPHLSLLATGNNLYAGYFSIFLVLIALFCREYRGTP
                     FFRIWVTMVLVALLMALGRWSPLYVALVKLTRFYSFRVPSKFLGFFCFGVAMLAALGF
                     RILWNGGVRSRVTKLALITLVMVSLAVFGGMTAGTVLLSRGRQFVLGLGEAYVKRFVY
                     GQPGHPHTLERYLSTVSEFPDRILKFLSWNEPANIGAVITALFCLLVILIIVRKKYSR
                     PVMLFGILFLVADLYGAAFFDIRGDLAAYKSALVSSPIVEILKKEKAAGNLGRVYGCR
                     APSQRLPLVPSQNMLYGIADIGAYSPLVMSRYYETIGCLGNINDSTLAVTPAPAFVTR
                     RLPLLDFLNVSHILSTVSLNHPNLELIFEDPLMSAYLYETGNSHKEAYFVTEVRFFEG
                     WEPLKAELLNEGFDPANTLLLEREGSNSFSGISRRGNAQSALIEKKVARVGFSSWELI
                     CSSPGFFVLPNLHFPGWKAEVNGEEVLILKAYGVFQAVRINAAGRYTVEFLYPTFYER
                     KFI"
     gene            1882..3753
                     /locus_tag="PK590_05550"
     CDS             1882..3753
                     /locus_tag="PK590_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24094.1"
                     /translation="MILDGLLAIFYWLLLLPVIYLLGSYAKRWIDEKSVLLHGIYIMG
                     FGFATMSYFMTVLGTFGLLKASVVFPGIIALAFIRIKNFREFRIWVLEVGRCLLPPPG
                     GRLNTVSHILLLGSLIITAALCSLPEIAHDSLCYHINLPKLFVQSGSVLPISYDLKSC
                     QSQFLEVLFSIGIFLNNIPAAKLFHWFCGFFALLFVIRFVEVESGNRGLALTMGLAFW
                     LTPTILNQVTVTYNDAGVTWFVLLSWGMFVSALRNGYRTRDFFLSGLFLGFATSCKYL
                     ALMFGVPMGLYLVWGVITQPDRRLALMKGIVFFSFGAFLACGYWFLRNYLFSGHPLYP
                     VFSAKQDVAGLYAEQNYASIGVPKTLLSFLTLPITMVISPQSFEKHFWAGPYYLIILP
                     LALFGAFRDKLARFAFFTVLISAILWFLIAQSIRFLLPIFPIFMIGAAIGFRELPVKS
                     WASRMQMVFRVLVAVAISILIAAALYHYRIHFLALVAGWSKDTFLTRLERTYPVAKWV
                     NENLSKDAKLFSASEIRQFYFNRPMIDAVSYYRVERYSDTLTPSEMVDRLKKNNITHV
                     LQVLPAGEGDGKQPRGSSPLDVVHAVVSDADLAVSTKEIPSQNIRESQFVYRIYELK"
     gene            3768..4115
                     /locus_tag="PK590_05555"
     CDS             3768..4115
                     /locus_tag="PK590_05555"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG24095.1"
                     /translation="MKISVVVPTKNEEINVGWIIESAKKYADEVIVIDGHSTDRTREI
                     AEACGVKVFLDNRKGKGDAIRVGIANVTGDIIVFIDADGSHLPEDIPSLVAPIKAGEC
                     DHVTGSRMRGGVR"
     gene            4078..4440
                     /locus_tag="PK590_05560"
     CDS             4078..4440
                     /locus_tag="PK590_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24096.1"
                     /translation="MSQGHECVGGSDELHGDIGKFIRMVGSDIITLGINYRFNVRLTD
                     SQNGFRAIRSDVARKLPLKENITTIEQEMIIKTLKLGYRMGEVPTHEYARRGGESKIS
                     ARKVAWRYVYSWLKYLFF"
     gene            4449..>4745
                     /locus_tag="PK590_05565"
     CDS             4449..>4745
                     /locus_tag="PK590_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24097.1"
                     /translation="MACKVLLINPPVPDNKVWVREGRCQQWDIWGAPFPPFSLAMIST
                     QLVKDGHETALIDSGPEKKDLNQVIEESKAFAPHVVILATTSPTIQSDLAWFMPA"
BASE COUNT         1001 a         1035 c         1266 g         1443 t
ORIGIN      
        1 ctggttcccc gtagccttgt atttgctgga gcgttacctg gaggggtata aatcgcgctg
       61 gcttttcggc ctggcggttg tggtggggca gtccatggta gctggctacg tgcagatggc
      121 ggcgtttacc tggataatct ttgccgtgta tgccgtactg cgggttctgt tcttcccgga
      181 ttcttcaggc ggctggattt ttcgaatagt attacttggg cgattggttc tttccgctgt
      241 gggagcggtt gggctcgccc tgcctcagct ttttttgacc taccaactgg cgatcctttc
      301 gaaccggacc ggtctcgaag aggggtatgc ctatgtcggc tcaatggcgc cttatatcct
      361 agggacgcta ttcaatcccc atttgtctct tttggcgacg gggaacaatc tttatgccgg
      421 atatttttcg attttccttg tcctgatcgc cctgttttgc cgggagtatc gcgggactcc
      481 gttctttcgc atatgggtca cgatggtgtt ggtcgctctt ctgatggctc ttggccgctg
      541 gagcccgctt tatgtggcac tcgtcaaatt aacgcgattt tattcattcc gggttccttc
      601 caaatttctc ggcttttttt gcttcggcgt ggcgatgctc gcggctttgg gattccggat
      661 tctgtggaat ggcggggtgc ggtccagggt tacgaaattg gccttgatca cccttgtaat
      721 ggtatcacta gctgtctttg gagggatgac ggcagggacc gttttgttgt cgcggggacg
      781 ccagtttgtc ttgggcttgg gagaagctta cgtaaaacgg tttgtttatg gccaacccgg
      841 gcatccgcat accctggaaa gatacctcag taccgtgagt gaatttccgg accggattct
      901 caaatttctt tcatggaatg agccggccaa tatcggagca gttattaccg cgttattttg
      961 tctcctcgta atactgataa ttgtccgaaa aaaatattcc cgtccggtca tgctcttcgg
     1021 gattcttttc ctcgtggccg acctttatgg ggccgctttt tttgatatac gtggcgatct
     1081 tgcggcgtat aaatctgctt tggtctcatc cccgattgtt gagattctca agaaggagaa
     1141 agcggcgggg aacctgggcc gagtctacgg atgccgcgcg ccgtcccaga ggcttccgct
     1201 cgtgccaagc cagaatatgc tttacggtat tgcggatatt ggcgcctatt cgccgcttgt
     1261 gatgtcccgc tactatgaaa cgatcgggtg tttgggaaac atcaatgatt ccacgcttgc
     1321 tgtgacgccg gcccctgcat ttgtcaccag acgtcttccg ctccttgatt ttttgaatgt
     1381 atcacatatc ctttccactg tttcgttgaa ccatcccaac cttgagctga tttttgagga
     1441 tccgctgatg agcgcctact tatacgaaac agggaactct cacaaggagg cttattttgt
     1501 gaccgaagta agattttttg aaggttggga gccgttgaaa gcagagttgc tgaacgaagg
     1561 gtttgatccc gcgaatacat tgttgttgga aagggaaggt agcaattctt ttagtggaat
     1621 atcccggcgc ggcaatgctc aatccgcttt gatcgaaaaa aaagttgctc gggtcggttt
     1681 ttcttcctgg gagctcattt gttcatctcc cggttttttt gttttaccca accttcattt
     1741 cccgggatgg aaagctgaag taaacgggga agaggttctg attctgaaag cttatggggt
     1801 gttccaggcg gttcggatca atgcggccgg tcgctataca gtcgagtttt tataccccac
     1861 tttttatgaa aggaaattca tatgatttta gatgggctgc tggcgatttt ttactggctc
     1921 ctgttgttac cggtgatcta tcttttaggt agttatgcaa agagatggat cgacgaaaaa
     1981 tcggttcttc tgcatggtat ttatatcatg ggatttggat ttgcgaccat gagctatttt
     2041 atgaccgtat tgggaacttt tggattgctc aaggcctctg tagtttttcc gggcatcatc
     2101 gctttggcgt tcattcggat aaaaaacttt cgagaattta gaatttgggt tctggaagta
     2161 ggtcgctgtt tactgccccc accagggggg aggctcaata ccgtgtcgca tatccttctg
     2221 ctcggatcgc tgattataac agcggctcta tgctccctgc cggaaattgc ccatgattcg
     2281 ctttgttatc atataaatct cccgaagctt tttgttcaaa gcggatcggt cctcccaatc
     2341 tcgtatgatc ttaagtcatg tcaatctcaa tttttggaag ttctttttag catcggcatt
     2401 tttttaaata atatcccggc cgccaagctt tttcattggt tttgcggttt ttttgccttg
     2461 ctgttcgtta taaggttcgt tgaagtggaa tctggaaatc ggggtcttgc attaacgatg
     2521 gggctcgcgt tctggctcac accgacgatc ttaaaccagg tgacggtgac ctataacgac
     2581 gcgggggtca catggtttgt tcttttatcg tggggtatgt ttgtgagtgc gctaaggaac
     2641 ggttatcgaa cacgcgattt ttttctaagc ggactctttt tggggttcgc gacaagttgt
     2701 aagtatctcg cgttgatgtt tggggttccg atggggttgt atctcgtctg gggcgtgata
     2761 acccagccag accggcgtct tgcgctgatg aaaggaattg tcttcttctc attcggtgca
     2821 tttctagcgt gcggctattg gtttttacga aattatcttt tttcgggaca tcccctttat
     2881 cctgtttttt cggcgaagca ggatgttgcg gggctttacg ccgaacaaaa ttatgcgtcg
     2941 atcggcgtac ccaagacact gctttcattt ctgacgctcc cgatcacgat ggtgatttca
     3001 cctcagagct tcgaaaagca tttttgggcc ggcccgtatt atctgatcat tcttccattg
     3061 gcgctttttg gagcgttccg tgacaagttg gcaaggttcg cgttttttac cgttttgatc
     3121 tcagctatcc tctggtttct tatcgcgcaa agcattcggt ttttgctccc aatatttccg
     3181 atcttcatga ttggtgccgc gattggattt cgcgaacttc ctgtgaaaag ttgggcttca
     3241 agaatgcaaa tggtgtttcg tgtgctcgta gcggttgcaa tttctatttt aattgcggcc
     3301 gctctctatc actaccggat acatttcctt gcgctggtgg cggggtggtc caaggatacc
     3361 tttctcacac gtttggaacg gacttatccg gtggcaaagt gggtcaatga aaatctttcc
     3421 aaggacgcta aattgtttag cgcgtcagaa attcgccagt tttattttaa tcgtccgatg
     3481 attgatgcgg tttcttacta tcgggttgag cgttattctg atacacttac gccttccgag
     3541 atggtggatc ggttgaaaaa aaataatatt acgcatgtgt tgcaagttct tccagccggc
     3601 gaaggagacg ggaaacagcc ccgtgggagt tcgccgcttg atgttgtcca tgccgtggta
     3661 agcgatgcgg acttggctgt ttccacaaaa gagataccct cacaaaacat tcgtgagtca
     3721 cagtttgttt accgcattta cgaactgaaa tgaaaaaagg aggggacatg aaaatttctg
     3781 tcgtggttcc gacaaagaat gaagagatta atgtcggatg gattatcgaa agcgcaaaga
     3841 aatatgcgga tgaggtgatt gtgattgatg ggcattccac ggatcggaca cgagagatag
     3901 ctgaggcttg cggggttaag gtttttttgg ataatcgcaa aggaaagggg gatgcgatcc
     3961 gtgttggtat cgcgaatgtt accggagata ttatcgtgtt cattgatgcg gacggatcgc
     4021 atttaccgga agatatcccg agcttggtgg cgccgatcaa ggctggggag tgcgatcatg
     4081 tcacagggtc acgaatgcgt gggggggtca gatgagcttc atggagatat aggaaaattc
     4141 attcgcatgg tgggtagcga cattattacg ctgggcatca attatcgttt caatgttcgt
     4201 cttacggata gccagaatgg ctttcgtgca attcggtcgg atgttgcaag gaagctgccg
     4261 ctcaaggaaa atattacgac gattgagcaa gaaatgatca tcaagactct gaagctcggc
     4321 taccgaatgg gggaggttcc cactcatgaa tacgcccgaa gaggggggga gtccaaaata
     4381 agtgccagaa aggtagcctg gcgatatgtt tattcttggc ttaaatatct gtttttctag
     4441 ttcccgccat ggcctgtaaa gttctcctga ttaatcctcc ggttcccgat aacaaggtct
     4501 gggtgcgtga aggacgttgc cagcagtggg acatctgggg agctccgttc ccaccgttta
     4561 gtttggccat gatttcgacg caacttgtca aagacggtca cgaaacggct ctgatcgata
     4621 gcgggccgga gaaaaaagat ctcaaccagg tcattgaaga aagtaaagct ttcgcgccgc
     4681 atgtggtcat cctggcaaca acatccccga cgattcaatc cgatctcgcg tggtttatgc
     4741 ccgca
//
LOCUS       DAOSIO010000130         4723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_16088_length_4723_cov_3.270994, whole genome shotgun sequence.
ACCESSION   DAOSIO010000130 DAOSIO010000000
VERSION     DAOSIO010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16088_length_4723_cov_3.270994"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2259)
                     /locus_tag="PK590_05570"
     CDS             complement(<1..2259)
                     /locus_tag="PK590_05570"
                     /inference="COORDINATES: protein motif:HMM:NF014565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STT3 domain-containing protein"
                     /protein_id="HOG24098.1"
                     /translation="MRLFSFSTKWAVLLVIGILAIFFRTYSLHGGPFMMFRDSETMAR
                     ELIDREMKGQIGSTLEKKVSGISDADKKSWVDYQLRNLAETGGDERYEAAVRRSASLI
                     ERVRKENWLGPRYLLEADPYHYLYQVEKILTDGFLTENFRSGRYFHPLMRAPHGHWGE
                     ALLHPYVGVFTYRLFQWFDPDVSLVRALGFVPLGLTLLILIGYGALGKSFGLGWKESA
                     FGMATLVLSPIFIQRSALGWFDTDPYNCLFPILILSCVMRSFRQPRYFWAGVWGAGFL
                     TALYGFFWNGWLFIFPLVLASILVGTMLLVLLRRSQSRALARTTCRFAALYLTISGLF
                     LLIFWSPERLSGMVRMAATLLKQFALADFDIWPNVFLTVGEAGGITLKKLVFLTGNYV
                     TFAVAFAGVLLEGWKSFRRDDIFRQFRYLFLLIFSLPLFFISLKTERFSLLFVLPLSL
                     FVSFGVARIVDLADSWWRRAGIFRHPDRIFARRLATYLLFLAGFLPLMLLTAHVVARN
                     ITPIMDDVWNAAMTDLREKTPHDAIINSWWPPGHFITGVARRRVTVDGGSQHFHVTYW
                     MARALMAENEREAAGIFRMLNLSGEEALLFLTEGGMEVPDAVDLLLKIVCLGRNEASL
                     ELPSSMTEEQKESLLDLTHGRGELPPSYVLVYDDLITQNLAVSTMAQWDFRKAMEIRE
                     ERRARSPVPMGFFDKNAIAGYMQDFLRASGEFAKYIAAAKLDRREGSLLLFSNGVAVN
                     LASQDAFIFLPAK"
     gene            complement(2387..3214)
                     /locus_tag="PK590_05575"
     CDS             complement(2387..3214)
                     /locus_tag="PK590_05575"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG24099.1"
                     /translation="MRTFALVVLFSCLFVPFAFSGISRDDYQRVMNLFEEGQYNESIA
                     ILKSSLDQDPNQAVLYNLLGMIYLKQGESIASAIGSFEEAIRIDPDYADAYFNLASTY
                     ASSANRPELAADYFKKTLEADPKYVRAYFGLGWFVLTTQEKPEEASEYFKKAVEYFPD
                     FSEAYYGLGLSYIQMGKAPLALESISKVRAMGREDLAYYLETILRGGKVSVPIPEDPL
                     AEVPLADNEALPSQETAPEWGRRTGFSEQAVNREDKISKEMTGQDEISENNNPFQLY"
     gene            complement(3216..3830)
                     /locus_tag="PK590_05580"
     CDS             complement(3216..3830)
                     /locus_tag="PK590_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24100.1"
                     /translation="MAKKAQRGKFDLNEMISRMSSQNRVLFVVTVIMVALALMDRLMF
                     GPFSSQMKYMEAENQAQMESLKRSHRIISFQDGIMEEYQKYSTYMDTGAMKQEEIVAA
                     LLKKIETLAAQQSVLITNVRPDDVEEKPNFRIYKTSIDCEGKLGNVLMFIHLLEQSDY
                     LFQVERYSMGPKSKGSDIIKCTLDIARILITVEKPTAALETGRF"
     gene            complement(3833..>4723)
                     /locus_tag="PK590_05585"
     CDS             complement(3833..>4723)
                     /locus_tag="PK590_05585"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOG24101.1"
                     /translation="TVLDKEQVVFVRHIPVGARTFRKDPEKFRVDFAQELTRSIGAYH
                     DQGIGRPVGKLLITGLVDDLGPVDDILNQVSPAPSGKIEVAGFDYRKYFTVSDLVMNH
                     LRQTPAVSFFDLFSVLAHSDQNKVDLLLPELKLRRKVRDVSRDILRFGVLIMAIFFLV
                     SLFMGSKIYFKKSLNQKLQRISHQYGEQAKTLEVISTKNRIVKKVIAKRGKELVVLNI
                     ISGFIGDDLYLSQINYDSAGTVSIAGTADSMSRVFSFVTQLKHSNYFRSVEAKETKSR
                     KEGKQDVADFLIDCELAEGF"
BASE COUNT         1376 a         1277 c         1007 g         1063 t
ORIGIN      
        1 tttcgcgggt agaaagataa acgcatcctg agacgcgaga ttcacggcta ccccgttaga
       61 aaaaaggaga agagagcctt cccgtcgatc cagctttgcc gccgcgatat atttggcaaa
      121 ttcgcctgaa gctctcaaaa aatcctgcat ataacccgcg atggcattct tatcaaaaaa
      181 acccataggc acgggactac gcgcgcgccg ctcttctcga atttccatcg cctttctgaa
      241 atcccattga gccatagtgc tcaccgcgag attttgcgta atgagatcat cgtaaacaag
      301 aacatacgag ggaggaagtt cacccctccc gtgggtcagg tcaagaagcg attctttctg
      361 ctcttccgtc atggacgatg gaagctccag ggatgcctcg ttgcgtccca gacagactat
      421 ctttagcagc aaatcaaccg cgtcagggac ctccattccc ccttccgtca ggaaaagaag
      481 ggcctcctct ccgctcaagt taagcatgcg aaaaatcccg gcggcctctc gttcattttc
      541 agccatcagt gcacgggcca tccagtaagt gacgtgaaag tgttggcttc caccatcaac
      601 cgtaacgcgc cggcgcgcga cgccagtaat aaagtgaccg gggggccacc aactgttaat
      661 gatcgcgtca tgaggcgtct tttcacgcag atccgtcatt gcggcattcc agacatcatc
      721 catgatcggc gtgatatttc gcgcgacgac gtgagcggtc aataacatca gcgggaggaa
      781 tcccgccaaa aacaaaaggt aagtcgcaag ccgtcgagcg aaaatccgat ccggatgccg
      841 gaaaattccg gcgcgacgcc accaactgtc ggcaagatcc acaatccgag ccactccaaa
      901 actgacgaaa agtgacaaag gtagaacaaa aagaagagaa aatctctcgg tctttagaga
      961 aataaaaaac agcggaagac tgaagatcag taaaaaaaga taacgaaatt gacgaaaaat
     1021 atcatcgcgc cggaaagatt tccagccttc taacaaaaca ccggcaaaag ccacggcaaa
     1081 cgtgacgtaa ttaccggtca gaaaaacaag ctttttcagc gtaattcccc cggcttcacc
     1141 aaccgtcaaa aaaacattag gccagatatc aaaatccgcc aacgcgaact gtttcagcaa
     1201 tgttgcggcc attcggacca tccccgacaa cctctccggc gaccaaaaaa tcaataaaaa
     1261 caatccgcta atcgtcagat ataaggccgc gaagcgacat gtcgtcctcg ccaaagcgcg
     1321 cgattgagac cttcgcaaca acaccaaaag catcgtgccc accaaaatgc tcgccaaaac
     1381 gagcgggaaa ataaaaagcc agccattcca gaaaaaacca taaagtgccg tcaaaaaacc
     1441 ggcgccccat accccggccc agaaatacct gggttgacga aaacttcgca tcacgcaaga
     1501 caaaataagg attggaaaaa gacaattgta ggggtccgta tcaaaccatc ccaaggcact
     1561 tctctggata aaaatcggcg atagaaccag ggtcgccatc ccgaacgcac tttccttcca
     1621 gccaagacca aaactttttc cgagtgcccc gtatccgatc aaaatcagga gtgtcaaccc
     1681 cagcgggaca aaaccaagag cccttaccaa agaaacatcc ggatcaaacc actgaaaaag
     1741 gcggtaggta aatactccca catacgggtg aagcaaggct tctccccaat gaccgtgcgg
     1801 ggcccgcatc aggggatgga aataccgtcc acttctgaaa ttctccgtaa gaaacccatc
     1861 cgtaagaatt ttttccacct gataaaggta atgataggga tccgcctcaa gcagataacg
     1921 ggggcccaac caattttcct tacggacgcg ctcaatgagg ctcgccgatc ttcttaccgc
     1981 cgcttcgtat ctttcatcac cccctgtctc cgcaagattc ctgagctgat aatccaccca
     2041 agattttttg tcagcatcgg aaatccccga aacttttttt tctaacgtac ttccgatctg
     2101 acctttcatc tcccggtcga taagttcgcg agccatagtt tctgagtcgc gaaacatcat
     2161 aaagggacca ccatgaagtg aataagttcg gaaaaaaata gcgagaattc cgatcaccag
     2221 gagaaccgcc cactttgtgg aaaaagaaaa aagacgcata aagtttcctc aaggcctacg
     2281 ggtacgcatg tctagattca accttaaaat atactcgtct gaagtatcgc caaaaacatc
     2341 taccttcagg ggaaacagat atatcagtaa atgacgggtc aaaaatctag taaagctgaa
     2401 agggattatt gttttccgaa atctcgtctt gaccggtcat ctccttcgaa atcttatcct
     2461 ctcgattcac ggcctgttcg gaaaatcctg tcctcctccc ccactccggc gccgtttctt
     2521 gagaaggtaa cgcttcgtta tctgccaaag gaacctcagc aagcgggtct tccggtatcg
     2581 gaacagagac ctttccgcct cgaagaatgg tttcgagata ataagccaag tcttcccggc
     2641 ccatggccct aactttggaa atcgactcaa gcgccagcgg cgctttcccc atttgaatat
     2701 aggaaagacc aagaccataa taagcttccg aaaaatccgg aaaatattca actgcctttt
     2761 tgaaatattc cgacgcttcc tccggtttct cctgggtcgt aagaacaaac catccgagac
     2821 caaaataggc ccggacatac ttcggatcgg cttccagagt cttcttgaaa taatcagccg
     2881 caagctcggg tctgtttgcg gaactggcat aggttgaggc aaggttaaag taagcgtccg
     2941 cgtaatcagg gtcgatccga atcgcctcct caaaagagcc gatggcgctg gcaatcgatt
     3001 cgccctgctt caaataaatc atcccgagca aattgtacaa gaccgcctgg ttcggatcct
     3061 ggtcaagcga ggacttcagg atcgcgatcg attcgttgta ttgcccttct tcaaacaggt
     3121 tcatgacccg ctgataatca tctcgcgaaa tcccggaaaa cgcgaaagga acgaaaaggc
     3181 aagagaaaag aacaaccagg gcgaaggtcc tcatatcaaa atcttcctgt ttccaacgcc
     3241 gctgtgggtt tttcgacggt gatcagaatg cgggcaatgt ccagcgtgca ttttatgatg
     3301 tcagacccct tggatttggg tcccatggaa taacgctcaa cctgaaacag ataatcggat
     3361 tgttccagca aatggatgaa cattaacacg tttccgagct ttccttcaca atcaatgctg
     3421 gttttataaa tccggaaatt cggtttttcc tccacatcat ccgggcggac gttcgtaatc
     3481 aaaacggatt gctgggccgc gagcgtttct atctttttta aaagggccgc gacaatttcc
     3541 tcctgtttca tggctcccgt atccatgtaa gtactgtatt tttggtactc ctccataatc
     3601 ccgtcctgga acgaaataat gcggtgacta cgcttgagac tttccatttg cgcctgattt
     3661 tccgcttcca tgtatttcat ctgggaactg aacgggccga acatcagacg gtccatcagg
     3721 gcaagcgcga ccatgatcac ggtcacgaca aaaaggaccc tgttttggga actcatccga
     3781 gatatcatct cgttcaggtc aaatttaccg cgctgggcct tttttgccat cgtcagaatc
     3841 cttccgccaa ctcgcaatca atcaaaaaat cggcaacatc ctgcttgcct tcttttcgcg
     3901 atttggtttc cttagcttca accgatctga aatagttaga gtgtttcaac tgcgtcacaa
     3961 aagaaaaaac ccgcgacatt gaatcggcag tgcccgcaat tgagacagtc cccgcgctat
     4021 cataattaat ctgtgaaaga tagaggtcat cgccaataaa tccgctgata atatttaaca
     4081 cgacgagctc ttttccccgc ttcgcgatga ccttcttaac aatccggttt tttgtcgaaa
     4141 tcacctcgag cgttttagcc tgttcaccat actggtgact gatacgttga agtttctggt
     4201 taagtgattt tttaaaatag atcttactcc ccataaacag actcacgaga aaaaaaatcg
     4261 ccataatcaa gacaccaaat ctcaaaatgt cccggctcac atcacggact tttcgccgaa
     4321 gtttcaactc cggcaaaagg agatccacct tgttttgatc cgaatgggcc agtacagaaa
     4381 agaggtcgaa aaaagaaact gctggtgttt gtcggagatg gttcatcacc aaatctgaaa
     4441 cggtaaaata ttttcgatag tcaaaccccg caacttcgat cttcccagac ggcgccggtg
     4501 aaacctgatt taaaatatcg tccaccggac caagatcgtc cacaagaccg gtaatcaaaa
     4561 gcttgccaac cggccgacca atcccctgat catgataggc cccgattgat cgcgtcagct
     4621 cctgggcaaa atcaacccga aacttctcgg gatctttccg aaacgtacgg gcgcccacag
     4681 ggatatgacg gacaaaaacc acctgctctt tgtccagcac cgt
//
LOCUS       DAOSIO010000131         4675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_16297_length_4675_cov_4.658009, whole genome shotgun sequence.
ACCESSION   DAOSIO010000131 DAOSIO010000000
VERSION     DAOSIO010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16297_length_4675_cov_4.658009"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1687)
                     /locus_tag="PK590_05590"
     CDS             complement(119..1687)
                     /locus_tag="PK590_05590"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG24102.1"
                     /translation="MFKSFSARISIIFFLTSAFVLGAVFFFFYFRAAKLRDDAFQKHF
                     ENFIEVSANFISGEEVLEIPLEEGCDKLPQGGNLIRKLRSIRAVDPDIFDVYVMVRGP
                     DRSTLRFVTNADRERTPVACGEPYDVRDEPRMRDGFLDAVANMIPSTDKWGTWVSAYA
                     PIRKRTGETVAMIGVDIAQQRIMEIRAEFFNLFLLSIGACLFFSLAIGLLSSFWLTRP
                     MRQVIQGMERVRSGDLNHSLGHFSQIEFEKIANIFNQMTGSLRHMIRELEISTRENER
                     VRHEIEVATEIQQSIFPENPPEIEGLEIAASSAPAKAVGGDYFDFFPVGTDAATRTGF
                     VIADAAGKGIPGTLYMTRSRSVFRVVSSQGNAPGETLSRSNSYIASDSSSKQGMFITV
                     LYMIYDSQEKQMIYSNAGHYNPLWFKSGEKKFIPLKSGGIPVGILPGQKFPEETLQLD
                     SGDILVMYTDGVIEAKAEDGQMFGLERLTRIIEQNSSLGAHEIFEKIQAEIRDFIGKA
                     LPFDDMTLMVLRVK"
     gene            1910..2704
                     /locus_tag="PK590_05595"
     CDS             1910..2704
                     /locus_tag="PK590_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOG24103.1"
                     /translation="MKNFGRWILSTWIASMIFFPSGTYDLVPEDLGLSYEAVALPISH
                     ATTLRNWFLPVPEDKGTLLFLHGNAGNISDRLFKAKGWVERGYSVFLLDYRGYGKSTG
                     KLHSENDILQDAREGWNWLTREKKISPDKIILYGESIGSFPAMVLASEHQARAIILEA
                     PFTSFKDLAPRHYPWVPGLILDIILRDFEFSNISRVQTLQTPLFIMHGTADEICGYEM
                     GLKLYEKALVTKEFFSVKDGGHNDLPFRAGQDFWSEPQKFIEAQTR"
     gene            complement(2809..3432)
                     /locus_tag="PK590_05600"
     CDS             complement(2809..3432)
                     /locus_tag="PK590_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOG24104.1"
                     /translation="MDLKKFRMKPLMGILRGLLPEELEPLFDTILLSGLETVEITLNT
                     PGAFKLIRRASRLYGKKLMIGAGTVLTVTDLRKALDAGATFIVSPVLVRPVARECVKR
                     KIPVFPGALTPREVYDAWTAGASMVKIFPSGCLGPGYFRELKGPFRDVELLACSGVTP
                     ENVGEYFRSGASAVAVGSGIFRRDWIQSRKFTLIRDKIQAYLKGFPE"
     gene            complement(3559..4512)
                     /locus_tag="PK590_05605"
                     /pseudo
     CDS             complement(3559..4512)
                     /locus_tag="PK590_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010538.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
BASE COUNT         1159 a         1308 c         1072 g         1136 t
ORIGIN      
        1 caggtttgtt tacaaattcg gccttttttt cactgcctta agtgggatac acccctgtct
       61 cccgggatac cgattcgccg gggcccgcgg cagagaaaaa acgttcggcg atcgcctact
      121 acttaacccg aagaaccatc aaagtcatgt cgtcaaacgg aagggccttg ccgataaaat
      181 cccggatctc agcctgaatt ttctcaaaaa tttcatgggc cccaagcgaa ctattctgtt
      241 caatgatccg ggtcaatctt tccaggccaa acatttgccc gtcctcggcc ttggcctcaa
      301 tcactccatc ggtatacatc acgagaatgt cccctgaatc cagctgaaga gtttcttccg
      361 ggaatttttg accgggcaaa atcccgacgg gaatgcctcc tgatttgaga gggataaatt
      421 ttttctcgcc tgatttaaac caaagcgggt tgtaatgccc cgcgttggaa taaatcattt
      481 gtttttcttg tgagtcataa atcatgtaaa gaaccgtgat aaacattccc tgtttcgacg
      541 aagaatccga agcgatatag gaattggacc gactaagcgt ctcgccgggc gcgttcccct
      601 gggatgacac caccctaaaa acactccggg accgggtcat gtaaagagtt cccggaattc
      661 ccttccccgc ggcgtcggcg atcacgaacc cggtccgggt ggccgcgtct gtcccaaccg
      721 ggaaaaaatc gaagtaatct cctcccaccg ccttcgcggg agcactgctg gcggcaattt
      781 ccagaccttc aatctccggc ggattttccg ggaaaatcga ttgctggatt tcagtcgcga
      841 cctcaatctc atggcgaacc ctttcgtttt cccgcgtgct gatttccaac tcccggatca
      901 tgtgccggag cgaaccggtc atctgattaa aaatgttcgc gatcttttca aactcgattt
      961 gagaaaaatg cccaagcgag tgattcaaat ccccgcttcg cacccgctcc atcccctgta
     1021 tcacctgccg catcggacga gtcagccaga aagagcttag caaaccaatc gccagggaaa
     1081 aaaacagaca agcccctatc gacagcaaaa acagattaaa aaattcggcc cgaatttcca
     1141 taattctttg ttgcgcgata tccaccccga tcatcgcgac cgtctctccc gtccttttcc
     1201 tgatcggagc gtacgccgag acccaagtcc cccatttatc ggtactgggg atcatgttgg
     1261 ccacggcgtc caaaaaacca tccctcatgc gcggttcatc ccggacgtca taaggttcac
     1321 cacacgccac gggcgtccgc tcccggtccg cgttggtgac aaatcgtaac gtggaccggt
     1381 ccggcccgcg gaccatgacg tagacgtcaa aaatgtccgg gtcaacggcc cgaatactcc
     1441 ggagcttcct gatgagattc ccgccttgcg gaagtttgtc gcaaccttct tccaaaggaa
     1501 tctccaaaac ctcttccccg gaaataaaat tggcgctgac ctcgataaaa ttctcgaagt
     1561 gtttctggaa ggcgtcatcg cgaagcttgg ccgcccgaaa ataaaaaaag aaaaagacgg
     1621 cgccaagaac aaaggcgctt gtcagaaaaa aaatgatgga aatccgggcc gaaaaacttt
     1681 taaacataat cctcctcgaa acctcttaac ttctctgaaa aatctcccat acgctatcat
     1741 gcccaatcaa accctggcca gagatttttc gccgggaata atccggtcct tctctcttcc
     1801 ctgaaacacg cgccggtccc ggctcacaaa acacacggca tcacggcttt atccggacgg
     1861 aaacgccaag tataatagcg cttggtaaaa atctcaaaga ggatccctga tgaaaaattt
     1921 tgggaggtgg atattgagta cctggatcgc gagtatgatc ttttttccga gtggaaccta
     1981 cgatctagta ccggaagatc tcggcctttc ttatgaggct gtggcgcttc cgatttctca
     2041 cgcgaccacc ttgcgaaact ggtttctccc ggtcccggaa gacaaaggga cattattgtt
     2101 tcttcacgga aacgcgggga atatttccga ccggcttttc aaggcgaaag gctgggtcga
     2161 acgaggctat tcggttttcc tcctcgatta tcgcggctac gggaaaagca caggaaaact
     2221 ccattccgaa aacgacatcc ttcaggacgc ccgcgaaggc tggaattggc tcacccggga
     2281 gaaaaaaatc tctccggata aaatcatcct ttacggagaa tcgatcggga gttttccggc
     2341 gatggtcctg gcttcggaac atcaggcgcg agccatcatc ctcgaagccc cttttacttc
     2401 tttcaaagac ctcgcccccc gtcattaccc ctgggtaccc ggattgatac tggacattat
     2461 ccttcgggat tttgagttct ctaatatcag ccgggtccag acactccaaa cgccactttt
     2521 catcatgcat ggcaccgccg acgagatttg cggttatgaa atgggcctga agctctatga
     2581 gaaggccctc gttaccaaag agtttttttc cgtaaaagac ggcggccata atgacctgcc
     2641 gttcagggcg ggacaggatt tctggtctga accacaaaaa tttattgaag cccaaaccag
     2701 ataaacctcg cccagggacc cgcccggcga agttaaaacc gcggtttttt ccttctcttg
     2761 atcacgattc cttgccagcg ccgcccgtgg agggcgcgag cgtttgaact attcaggaaa
     2821 acccttgagg taagcctgaa ttttatcgcg gatgagggta aactttcggg actggatcca
     2881 atctcttcga aaaataccgg acccgacggc cacggccgag gcgccacttc taaaatattc
     2941 cccgacattc tcaggagtca caccggagca ggccaaaagt tcaacgtctc ggaagggtcc
     3001 tttcagttct ctaaaatagc ccggcccaag acagccggaa ggaaaaattt taaccatgct
     3061 cgcgccggcg gtccacgcgt cataaacctc ccgcggcgtg agggctcccg ggaaaacggg
     3121 aattttcctt ttcacgcatt cccgcgcgac cggccttacc agcacggggc ttacgataaa
     3181 ggtcgctccg gcatccaggg ctttcctgag atccgtaacc gtcaacaccg tcccggcccc
     3241 gatcataagt tttttcccgt aaagccggga agcccttcgg atcaatttaa aagcgccggg
     3301 agtattcagg gtaatttcga cggtttcaag tccgctcagg aggatcgtat caaaaagagg
     3361 ttccagctct tccggtaaaa gccccctcaa aattcccatc agaggcttca tccgaaattt
     3421 tttaagatcc atacgaaaca ttttggcccg attgagtttg tgaacgcttt cgttgtgtgg
     3481 aaatgtctca aaatccaatc aacctcgatc tccggcgttt cggtctacgc tgaagaaaaa
     3541 cagagatccc cgtttaattt aatcgaggtt ggaagtgccg ccaaggctat cgtaaaactc
     3601 gcggtaatgg tcctttttgt cggtcttccg gatgtccagc gccgctttgg gatgctcatc
     3661 caaaattccc gtgatcgcgt aagggatcac atagccccac tccacctctt tggaaagcgg
     3721 gaccattttc ttcgcgatca catccaggac gggacgaatg ttaaattttg gatttttaag
     3781 aaatccgagc agaagttccg tgggacagtt gccagcggcg cgccccatgc cgtaaagggt
     3841 gctgtccaga taattggctc cgtggatgat ggcctcaatg gtattgccaa aggccaactg
     3901 ctgattgtta tgcgcgtgaa tgccaatctc cttggaccgg atgatgctct gggctttttt
     3961 gaccaagtac tcgaccgtct cctggtacaa cgcgccatta ctgtccacca ggtaaatcac
     4021 ctgcgcccgg cactcttttt caagctgttc gaaacattcc atgagctcca cttccagaac
     4081 tttggaaacc gccatcaagt tgacagtggc ctcatagccc ttatccacga aacagttggc
     4141 catatgaatg gccttgtcca cgtccttggc gtaacaggcc gttcgtatca tcgaaacagg
     4201 gctgtccttt ttcggtttga tgtcgtccat gtccacgcga tcgacatcta ccatcaccga
     4261 tattttggtc ctggattcaa ttcattcggt aacgcggcga atggtctcat catcgcaaaa
     4321 tttccaaggt ccatattttt tgtcgtcaaa aagtctccgc gaattcttgt atccgatttc
     4381 catataatcg ataccggcct gcgaaatagc cttgaacacc tcccgcacaa aactttcatc
     4441 aaacgcgtga ttgttcatta agcccccgtc acggatcgtg cagtccaaaa ccttgatttg
     4501 ctcccggtac atgtgaagtc tccctttctc aaaccttgcg gtgtgtattt ctggaatgaa
     4561 aatgactgaa gaaggtggca ttatactcct ccatcctctc tctcgtctac ccgcttgaaa
     4621 atcggggtgt atcccacttt aggcagttaa tttcttgagg cctgtaggag gctgc
//
LOCUS       DAOSIO010000132         4653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_16387_length_4653_cov_3.753806, whole genome shotgun sequence.
ACCESSION   DAOSIO010000132 DAOSIO010000000
VERSION     DAOSIO010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16387_length_4653_cov_3.753806"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            390..1442
                     /locus_tag="PK590_05610"
     CDS             390..1442
                     /locus_tag="PK590_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOG24105.1"
                     /translation="MLLLDRLLGFFSSDIGIDLGTANTLVYLKGQGVVLCEPSVVAID
                     RATHSALAVGEEAKRMLGRTPGNITAIRPMKDGVIADFDITEAMLRYFIRKVHRRHSP
                     VGPRVVVAVPSGITEVEKRAVKDSAERAGARTPVFLVEEPIAAAIGVGLPIHEPAGNM
                     IIDIGGGTTEIAVISLSGIVFAKSIRIGGDEFDESIISHLKKAYNLMIGERTAEEIKI
                     RIGSAYPLEEETTLDVKGRDVLAGLPKTISITSEEIREALSEPLSSILESVRIALERT
                     PPELSADLIDRGLILAGGGSLLKGLDKLISEETGLPVHIADDPLTAVALGTGRYLSDF
                     TLLKRLSVNTGSAREY"
     gene            1627..2445
                     /gene="mreC"
                     /locus_tag="PK590_05615"
     CDS             1627..2445
                     /gene="mreC"
                     /locus_tag="PK590_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOG24106.1"
                     /translation="MGDTVLIRRWRSLGWLGGALLILLTGFQFPRIHQSVRNIFDSVS
                     KPVLFAGSSVRNTASALRLNFGKFWDAVSKAREYQEQILDLQTRLLRYEEMDKENQRL
                     RKLLDFSQALPYKTVGARVIGEDNTPWKRVVILDKGSNRGIRKDMVLVAPEGLLGRVL
                     EVGPYTSRAIFLPDPQCRVSALTANGRVQGVISGTGSEILQMKYLPIDSEITIGEEII
                     TSGIESLFPKGIQIGTVVSLEKDPDGLHLRAQVRPAASFSKIEELLCLISPASK"
     gene            2418..2936
                     /gene="mreD"
                     /locus_tag="PK590_05620"
     CDS             2418..2936
                     /gene="mreD"
                     /locus_tag="PK590_05620"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOG24107.1"
                     /translation="MPHLTSLKVILYFAFLSVLDHAVLPVFGIGGVYPSFLYLFVCYA
                     SFEWETKKTIPVAFWAGLLKDFIGGELLGVQAGILVAMALLLDQVVRRIEREFPGIYF
                     FITLLYVVFCELLKLSFGMFIGRVDGVLASHIGTIILMGVYTALLLPFFGPLTRFAIR
                     DRALSRQYELFK"
     gene            2933..>4653
                     /gene="mrdA"
                     /locus_tag="PK590_05625"
     CDS             2933..>4653
                     /gene="mrdA"
                     /locus_tag="PK590_05625"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOG24108.1"
                     /translation="MRISRFYLLQAVVFLALLFLALSLFHVQVLKGHYYRDLSIRNHI
                     RLIPIEASRGRVFDRSGRLLATNRVSYDVVAFPEDVTPEVYPTLAKLLDMKEKEVRYR
                     MSAGREYPFAPALIKPDIPKSLAILIEERKPELPGVAIRIGGVRHYPYGETASHVIGY
                     IQAINGEEYERLDRDRFGIHSMIGRMGIEKYFDDTLRGWRGGEQIEVDARGRMVGVLS
                     RRPAEPGSDLHLTLDLDFQQKIMELIKDQRASVAVIDLETEGLLAMASSPAYDPNIFV
                     SPSASARRLSVLTDVQSPMIDRSIGAAYPPGSIFKLVTALAALEVGKITPQTRFFCNG
                     RFKLGKSGRVARCWFEEGHGSINLYQAIERSCNVYFYNVATKLTPEEIAKYAHELGLG
                     EPMRLEISKLAPGLIPDSAWKRARFKQPWYQGETINMAIGQGYVLVTPLEILRLTSII
                     AKNGKFVDPHLVQNNEYEESSRDRVAIREENLDVIKKAMLQVVESDYGTGQLARVDFG
                     KLAAKTGTAQTPPLKAHGWMTGFFPYKDPKIAFVVFVEHGGSGGIAGARIVKQLIELW
                     KGSYVPK"
BASE COUNT         1007 a         1101 c         1288 g         1257 t
ORIGIN      
        1 aggcactact ttcgtttgat aagcctcagc gcgccgttac tcccggtcaa tttgcggttt
       61 gttaccgccg aaatgtttgt gtggcggcgg gagaaatcca ggggatttac cgctcctgac
      121 cgtttccccg aagttgggaa agggcctcgt ctttcatgct ttcaaggttt aggtgcgttg
      181 gagcaatctg acgtgacata ggccgtattt ctccctggct gtggtgagcg gaaagacgga
      241 ccctttttat tcactcccgc atagattatt acacaattca tggcagacta accgggaacc
      301 gattttggtt tgacagggca gacggctttc gcttatacta ccgcctctac aaaattgatc
      361 ccacacacta acgaaaaagg cgttcatata tgctactttt ggacagactt ttaggctttt
      421 tttcctccga tattggtatt gatcttggaa ctgcgaacac gttagtttat ctgaaaggtc
      481 aaggcgtggt tctttgtgag ccctcggttg ttgcgattga ccgggcgacc cactcggctc
      541 ttgccgtagg tgaagaagcc aagcggatgt tgggacgaac ccccggtaat attaccgcta
      601 ttcggcccat gaaggacggt gtgattgccg attttgacat caccgaagcg atgctccggt
      661 acttcatccg caaggttcac cgccgtcaca gtccagtggg accgcgtgtt gtcgtggccg
      721 tgccgagcgg gattaccgaa gttgaaaaaa gagcagttaa agattccgcg gagagggctg
      781 gcgcgcgaac cccggttttt cttgtcgagg aacctatcgc ggccgccatc ggtgtggggc
      841 ttccgatcca tgaacctgcc ggcaacatga ttatcgatat tgggggtggg acgaccgaga
      901 ttgccgtgat ctccctgtcc ggaatcgtat ttgctaaaag tattcgcatc gggggcgacg
      961 agtttgacga atcgattatc agtcacctga agaaggctta taacctcatg ataggcgagc
     1021 gcaccgccga agagatcaag attcgaattg gttcggcgta tccgcttgag gaagaaacca
     1081 cgcttgatgt gaagggacgt gatgttcttg ccggtcttcc caagacgatt agtatcacca
     1141 gcgaagagat tcgggaagct ttgtcagagc cgctttcctc cattcttgaa tcggtgcgta
     1201 tcgcgcttga gcgtacccct cccgaacttt ctgcagacct tattgaccgt ggcttgattt
     1261 tggcgggagg tggttccctt ctcaagggac ttgacaaatt gatcagtgag gaaacaggtt
     1321 tgcccgttca cattgcggat gatccgttga ccgcggtagc gctcggaacc gggcgttatt
     1381 tgagtgattt taccttgtta aaacgattgt ccgtgaatac gggttcggcc cgggagtact
     1441 agcgctagtg tacggtcccg cttggatcgt ctgagtttta gtcggcttta acttgccgga
     1501 tcgcagctgt tcgtgacgta gatcgaaaaa acctcttcgt cctgcttctg atttgggttc
     1561 gggtttcctc aaacttgtct ccttccggtc cgtgatgttt aaagaacagt tttattttct
     1621 gaataggtgg gggacacagt gctgattcgc cgttggcgtt cgctgggttg gttggggggc
     1681 gctttgctga ttttgctcac cgggtttcag ttcccgcgca tacatcaatc cgttcgaaat
     1741 atttttgact ccgtttcaaa acccgtcctt ttcgcaggtt cctccgttcg aaataccgcc
     1801 agtgccttac ggttgaattt tgggaaattt tgggatgccg tttccaaggc gcgggaatat
     1861 caggaacaaa ttctcgacct ccagacacgg ttgttgcgat atgaagaaat ggataaggaa
     1921 aatcaacgtc ttcggaaact tctcgatttt tcgcaagcgc ttccgtacaa gaccgttggc
     1981 gcccgggtga tcggagagga caacaccccg tggaaacgtg tggtgatact ggataaggga
     2041 tccaatcgcg gcatccggaa ggatatggtg ctggtggctc cggaagggct tttgggccgg
     2101 gtactcgaag tagggcctta tacgtcacgg gctatttttt tgccggatcc acaatgccgg
     2161 gtcagcgccc tgacggcgaa cggtcgcgtt caaggggtga tttccggaac cggttccgag
     2221 attttgcaga tgaaatatct tccgattgat tctgaaatca cgattggtga agagatcatt
     2281 acctcgggaa tcgaaagcct ttttccgaaa ggaatccaga tcggaacggt tgtttccctg
     2341 gaaaaagatc ccgacgggct tcatcttcgc gcgcaggttc ggccagccgc gtctttttcc
     2401 aaaattgagg aattgctatg cctcatctca ccagcctcaa agtaatcctc tattttgcct
     2461 tcctgagcgt gctggatcat gccgtgcttc cggttttcgg gatcggaggg gtttatccgt
     2521 catttcttta tctttttgtt tgttacgcgt cgtttgaatg ggaaacaaaa aaaacaattc
     2581 cagtcgcgtt ttgggccgga cttttgaaag actttattgg cggggaactc ttgggtgttc
     2641 aggcgggtat cctggtggcc atggcccttt tgctggatca agtggtccga agaattgaac
     2701 gggaattccc cgggatttat ttttttatta ccctcttgta cgtggttttt tgtgagcttc
     2761 tcaagctgtc ttttggcatg tttattggcc gtgtcgatgg tgttcttgcg tcgcacatcg
     2821 gtacgattat cttaatgggg gtttatacgg cgcttttact tccgtttttc ggtcctttga
     2881 cacgttttgc gataagggat cgtgcccttt cccgacaata cgagcttttc aaatgagaat
     2941 atcgagattt tatcttcttc aggcggttgt ttttttagcg ttgctttttc tggcgctgag
     3001 tttgttccat gttcaggtgt tgaagggaca ttactatcgc gatttgagta ttcgcaatca
     3061 catccggctc attcccatcg aggcctcccg cgggcgggtt tttgaccggt cgggacggtt
     3121 gcttgcgacc aaccgcgtct cttatgacgt cgttgccttt ccggaagacg tgaccccgga
     3181 agtttatccc acgcttgcca agctccttga tatgaaggaa aaagaggttc gttaccggat
     3241 gagcgccggc agggaatatc ctttcgcgcc ggcactgatc aaaccggaca ttccaaaatc
     3301 tctggctatt ttgattgaag aaagaaagcc ggaacttccg ggagtggcga tccgtatcgg
     3361 cggcgtccgg cattatcctt acggtgaaac agcctctcat gtgatcgggt atatccaggc
     3421 gattaacggg gaggaatacg agcgtcttga ccgggatcgc ttcgggatcc attccatgat
     3481 cgggcgtatg ggaattgaaa aatattttga cgatacgtta cggggttggc gagggggaga
     3541 acaaattgaa gtggacgcgc ggggacggat ggtcggagtt ctctccagac ggcctgccga
     3601 gccgggttcg gatttgcacc ttacgctgga tcttgatttt caacagaaaa tcatggagct
     3661 gatcaaggac cagcgcgcga gcgttgccgt gatagatctt gagaccgaag gactcctcgc
     3721 gatggcgagt tctcctgcgt atgacccgaa tatttttgtc agtccatccg cgagcgcgcg
     3781 gaggctttcc gttcttacgg atgttcaaag cccgatgatt gaccgttcga tcggcgcggc
     3841 ctatcctcct ggatctattt ttaaactcgt gacggcgctg gccgctcttg aggtggggaa
     3901 aatcaccccc cagacccgtt ttttttgtaa tggacgtttc aaactcggca agtcgggaag
     3961 ggtagcccga tgttggtttg aagagggaca tggcagtatt aacctgtatc aggcgattga
     4021 gcgatcctgt aacgtttatt tttataacgt tgccacaaag cttacgccgg aagagatcgc
     4081 aaaatacgcg catgagcttg gtctcgggga acccatgcgt ctggaaatca gcaagctggc
     4141 gcccggcctg attccggaca gcgcgtggaa gcgggcgaga tttaaacaac cctggtatca
     4201 gggcgaaacc atcaatatgg cgattgggca agggtatgtg ctcgtgaccc cacttgaaat
     4261 tctccgtctc acctcgatca tcgccaagaa cggaaaattc gtggatcccc atctcgtcca
     4321 gaacaatgaa tacgaagaaa gctcccgcga tcgggttgcg atccgtgagg aaaatctgga
     4381 cgtgatcaaa aaggccatgc ttcaggtcgt ggagtcagac tatggcacgg gacagttagc
     4441 tcgggtggat tttggcaagc ttgccgcgaa gaccggaacg gcccaaaccc cgcctcttaa
     4501 ggcccatgga tggatgaccg gatttttccc ctacaaagat cccaaaattg catttgtcgt
     4561 atttgtggaa catggtggct ccggcggaat tgcgggcgcc agaatcgtca aacaactgat
     4621 tgagctctgg aagggaagtt atgttccgaa aat
//
LOCUS       DAOSIO010000133         4551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_16818_length_4551_cov_3.252224, whole genome shotgun sequence.
ACCESSION   DAOSIO010000133 DAOSIO010000000
VERSION     DAOSIO010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16818_length_4551_cov_3.252224"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1441
                     /locus_tag="PK590_05630"
     CDS             <1..1441
                     /locus_tag="PK590_05630"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOG24109.1"
                     /translation="QKTGFRIELGKLQFEPGAQTRLTVEMLKCYNPQSLEKLLQSDMI
                     TAELDLTSLLRKRFWMPTVKIQNPEVFLRRRSEGAWNWQREAKSETSLPPDQAGSQSG
                     TNGDSGGKVAPDGKLVSIPGGTPKIPISWEFGLGRLDVDDGTIHYVDETIQPSFALDI
                     VKCDVEILSNAALSAIRFVVHASVYNVVERKIELEGNWDFVSAMVEFNGNYDKDRASM
                     KGLLKLVGSVPKLEGALQVRDLDLEGVIPETHKTREYMSGILNTDLRLTCEGGNPDTI
                     FRSLTGAGSLRVENGAVRNRNIVKEVMEKIPVVTPSVQPGTDLPPEIGDLMNNSHTEF
                     QKVTVNFSVDHGKTTLQEFFLIHPNYQLNGQGDYGLLDKHINLDGMLVFSRSISELFV
                     KRVREIGFLRDRNGLLAVPFSYGGVLPDATVLPDLQYIGNKVLQGGAQELITKGLEKL
                     SQILEPIRQAPQEPVQKTNLASGKKGEKE"
     gene            1792..>4551
                     /locus_tag="PK590_05635"
     CDS             1792..>4551
                     /locus_tag="PK590_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24110.1"
                     /translation="MFTACHADQKPVALRAVSLFVIFVFLFTQSDVQLVSAYSVPHVP
                     QVDLSAADSGDLDKINYMQDFENLQQSVKDQTQSPLTKVAAGQEEQPIPIAPQLSDET
                     TPDITTNFLSQINPLLNSESKGEPTVETDNDVVTYRYPDNTHFSYQNSTGEIIEICDF
                     TKAVTNETTGETEYVLETRQFDYSVSGKVTVTTLATGEAEDAYQTFSLEDDGSLGVLL
                     ETGYVISDTNVRKCVRMREYGDDRMIVYNNVTETVGGVSTKFLIVQEYEKAPDSGDYR
                     LVRYTKGSANEDGLMLYQMDLEIRYDDDAGTLTVIDRNPDTQQVNRSFWEYALEPGHV
                     LGGLLAIGEMNLEKNTKIYRIGYGEGTYTMTRADDPKYILVFERLANDGFGRILRYRN
                     SEHDVEFRYERDSETGTETVTILNYANNTFIKMGFVPGASPAEDASGLLDSPENIVNA
                     GTFTLVGTVPTYKNILTRSGSEWIVEDPEDGRIFDVYSSFPSAEWGGLIRSRGPPEAD
                     ATTFADYLYEYDTAGRLVSVLDLTHLVYAVYDFTNSGEPRLTEQGTIAIDSSKHVTSK
                     TPTRVYSEDVALPFATTPPVPDDLNAYAAAQEALQTLIPDIDVSAISRAPISSSLVNP
                     DSLTVVLTYSDVQYFFSYDIQNQKAVLVKKRTPDWGTKNFTVVEYDDEGRQIRESKED
                     EHGVLTFLKEYRYHLEDKIVTIDFTNRVYTITVSTLNDDGTPGETLQTGFFGSYNANG
                     PCYSIGDVDADPASWKWYDYGEDGIMVTTDDRETSAPSRGVGVGTAGPGGEGYSTDDL
                     YRRLIRRVRDYKECTEEKSRATGSTALKCEEILDEVGALIRKLEDEQYRSLGGARKGE
                     DGGGGPNDLNYHTNTFVPEAILPTLIGQGPAPVDAVQIGTDADGNAILSWGGYIGVWK
                     AGASGL"
BASE COUNT         1178 a          972 c         1253 g         1148 t
ORIGIN      
        1 ccaaaaaacc gggtttcgca tcgaactcgg gaaactccag tttgaaccgg gcgcccagac
       61 acggctcact gtcgaaatgt tgaaatgcta caaccctcaa agcctggaaa aactgctcca
      121 gagtgacatg attacggccg agcttgattt gacgtctctt ttgcgaaaac gattctggat
      181 gcccaccgtt aaaatccaaa atccggaagt gttccttaga aggcgatcag aaggggcgtg
      241 gaactggcaa cgcgaagcga aatcagagac gtctctccct ccggaccaag cgggttcaca
      301 gtctgggacg aatggcgatt ctggggggaa agttgcgccg gatggcaagc ttgtgtcgat
      361 cccgggcggg actcccaaaa tcccgatttc ctgggagttt ggtcttgggc ggcttgatgt
      421 ggatgacgga acgattcatt atgtcgatga aacgatacaa ccctctttcg ctcttgatat
      481 tgtcaagtgt gatgttgaga tcctgtccaa tgccgcgttg tcggcgattc gttttgtggt
      541 gcacgcgtcg gtttataatg tggttgagcg caagatcgaa ctcgagggga attgggattt
      601 cgtgtcggcg atggttgagt ttaatggaaa ctatgataaa gaccgcgcgt ccatgaaagg
      661 acttttgaag cttgtgggat cggtgcctaa gctggaaggg gcccttcaag tgagggatct
      721 cgatcttgaa ggagtgattc ctgaaaccca taaaacccgt gaatatatgt ccggcatttt
      781 gaataccgat ctccggctta cctgcgaggg aggaaatccg gataccattt ttcgttcact
      841 gacgggcgcc ggaagtctcc gtgtggaaaa tggggccgtc agaaacagga atatcgttaa
      901 ggaagtgatg gagaaaattc ccgtggtgac cccatcggtt cagccgggaa cggatctgcc
      961 gccggagatt ggtgatttga tgaataactc gcatactgaa tttcaaaagg tgacggttaa
     1021 tttctcggtc gatcacggaa agacaactct ccaggaattt tttctgatcc atccgaatta
     1081 tcaattgaac ggacaagggg actacggtct tcttgacaaa catatcaatc tcgacggcat
     1141 gcttgttttc tctcgttcga tctcggaact ttttgtaaag cgagttcggg aaatcggttt
     1201 tcttcgtgac cggaatggat tattggcggt tccattcagt tatgggggag ttctccctga
     1261 cgcgacggtt cttcccgatt tgcaatatat cggaaacaag gttttgcagg gtggggccca
     1321 ggaactgatc acaaaagggt tagaaaaatt gtcgcagatt cttgaaccca tccgacaagc
     1381 cccccaagag ccggtccaga aaacgaatct ggcatccggg aagaagggag agaaagagta
     1441 atcgcgttca cgaagagttc aattttgtta acaagtcgct tcacaagttc atgtttgatt
     1501 gtgcaaaaac aatgcgctga actatagaag atgttaaggg ttttaataat tttttttctc
     1561 cctgtaatac cgttacttga gtggaaataa caaaatataa caatatatag catgtcgaaa
     1621 tttgacaagg aaacaggatg cagatatact ttcatcagag tttttaaaaa gtaaaaataa
     1681 acgataatgg cgaaggcgaa tgcgaaactt gaatgaaagt ggcgacaaga agagcgggca
     1741 gtttctctcg ttgctttttt cgtgcggtga ggaaaggagg ttctttttat aatgttcact
     1801 gcgtgtcatg ctgatcagaa accggtggcg cttcgggcgg tcagtctttt cgtcatcttc
     1861 gtcttcttgt tcacgcagag cgatgtacag ctcgtgtcgg cttacagtgt tccccatgtt
     1921 ccccaggttg atttatcagc ggcagattct ggcgatcttg acaaaattaa ttacatgcaa
     1981 gattttgaaa atttacagca gtctgtgaag gaccaaacgc aaagtccttt gactaaggta
     2041 gccgccggac aggaagaaca gcctatcccg attgcccctc aactttctga tgaaactact
     2101 cccgacatca caaccaactt tctttcacaa attaatcctt tgctgaattc tgagtctaaa
     2161 ggcgagccta ccgttgaaac ggacaatgat gtggtgactt accgttaccc ggataacacc
     2221 cattttagtt atcaaaattc cactggcgag attattgaga tttgtgattt tacaaaggcc
     2281 gtcacgaatg aaacgacggg tgaaacggaa tatgttctgg aaacgcggca gtttgattat
     2341 tcggtgagcg gtaaagtgac ggttacgacg ctcgccacgg gtgaagcgga ggatgcttat
     2401 cagacttttt cgttggagga tgatggaagt cttggcgttt tacttgagac cgggtatgtg
     2461 atttctgaca cgaatgttcg caagtgtgtt cgcatgcgtg agtatggcga cgaccgcatg
     2521 atcgtttata acaatgtgac ggaaacggtt ggaggggttt caactaaatt tttaattgtc
     2581 caggaatatg aaaaggctcc ggatagcgga gactatcggc ttgttcgtta cacaaagggg
     2641 agtgcgaatg aggatggatt aatgctgtat cagatggacc ttgaaattcg ctacgatgat
     2701 gacgccggta cattgaccgt cattgacaga aatcctgata cccaacaagt gaaccgtagt
     2761 ttctgggagt atgccctgga acctggccat gtcctggggg gtttgctggc catcggcgag
     2821 atgaatcttg agaagaatac caagatttac cggattgggt acggtgaagg tacttacacc
     2881 atgacccgtg cggatgatcc gaagtacatc cttgtgtttg agcgtttggc gaacgacggg
     2941 ttcggcagga ttttaagata ccggaacagt gagcatgacg tcgagtttcg ttatgagcgg
     3001 gacagtgaga cagggacgga aacagtgaca attttaaatt acgcgaacaa caccttcatt
     3061 aaaatgggtt ttgtccctgg agcgtcaccg gccgaagatg cgagtggttt gctggatagt
     3121 cctgagaata ttgtgaacgc gggaacgttt acccttgtgg ggaccgtacc cacgtacaag
     3181 aacatcctga cacggagcgg tagtgaatgg attgttgaag atcctgaaga tggcagaatt
     3241 tttgatgtgt actcgtcgtt cccaagtgcc gagtggggtg gattgatccg tagccgcggc
     3301 ccccctgagg cagacgcaac aaccttcgcc gattatcttt atgagtatga tactgctggt
     3361 cggctcgttt cagtccttga tctaacccat cttgtttatg cggtctatga ctttacgaat
     3421 tcaggggagc cccgtctcac cgaacaaggg actatcgcca tagacagcag taagcatgtg
     3481 acgtccaaaa cgcccactcg tgtctatagt gaggacgttg ctttgccttt cgcgaccact
     3541 cctcctgtgc cagatgatct gaatgcttat gctgccgcgc aggaggccct tcaaacactc
     3601 attcctgaca ttgatgtttc cgcgatctcg cgggcgccta tcagttcgtc actcgtcaat
     3661 ccggattccc tcacagtcgt cctgacttac agcgatgttc aatatttctt ttcttatgac
     3721 attcagaacc aaaaagctgt tttagtcaaa aagcgcacgc cagactgggg aacgaaaaat
     3781 tttacggtgg ttgaatatga cgatgagggg cgtcaaattc gggaaagcaa ggaagatgag
     3841 catggggtgc tgaccttttt gaaagaatat cgttaccatc tggaagacaa gattgtgacc
     3901 attgatttta ctaatagggt ttacacgatc acggtttcaa cattgaatga tgatggcacg
     3961 ccgggagaaa cgcttcagac gggatttttt ggaagctata acgcgaacgg tccctgctac
     4021 tcgataggag acgtggatgc cgatccggcg agttggaaat ggtatgacta cggcgaggac
     4081 ggaatcatgg ttacgactga tgaccgggaa acgtcggcgc catcccgagg tgttggtgtc
     4141 ggaaccgcag gacccggtgg tgaggggtac tccacggacg acttgtaccg gcgattgatc
     4201 cgacgggttc gagactacaa ggagtgcacg gaagaaaaat caagagcgac gggatcgaca
     4261 gcgctcaagt gcgaagagat tctggatgaa gtaggcgccc tgatccggaa gcttgaggac
     4321 gaacagtatc gttccctggg gggtgcgcgt aagggtgaag acgggggagg ggggccgaat
     4381 gacttgaatt atcacacgaa cacgtttgtt ccggaagcga ttctcccgac gttaattggg
     4441 caggggccag ccccggtgga tgccgtccag atcggaacgg atgcggacgg aaatgcgatt
     4501 ctttcctggg gcgggtatat cggtgtctgg aaagccggag cgagcggcct g
//
LOCUS       DAOSIO010000134         4521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_16940_length_4521_cov_3.619346, whole genome shotgun sequence.
ACCESSION   DAOSIO010000134 DAOSIO010000000
VERSION     DAOSIO010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16940_length_4521_cov_3.619346"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..1633
                     /locus_tag="PK590_05640"
     CDS             239..1633
                     /locus_tag="PK590_05640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG24111.1"
                     /translation="MRITKKTPALEIYQQAYQLYQIRILTILTLIPLYILTNRWGRFD
                     VNIWPLILVILIEVFINRPYRFLYTDSESSYRAMVGSVIIDFVAETTLLHLLGDIDFF
                     IYGVCYFITMVYCALRFPAFMTLLFATLASFLYVGLLALGQAGLIPLTISFGPEVTRT
                     EQVGFVVRQIAFFYLIGILVRFVAKAMTQKEEHLEELIWELKSTNEKIKYAHHLQTDY
                     FAKMSHEIRAPLNSIMGFSQLLLENTTGQLTEKQRDFIARIERSGSHLRDMINDVLDI
                     SKIESKKMTLSLREVNLVKVIDSVFDFFYEEASLKKILFAVTDKPKEPLLVTCDELKI
                     RQILYNLLSNAIKFTPSGGHIAMSLKKGPEGAIICIDDSGPGISPEFQKVIFTPYEQT
                     GRTTNTESKGTGLGLAISKQFVTMHGGKIYVKSEPGQGSQFYVEIPFRAKPLSGNKPA
                     ASGNFPEQFGSINP"
     gene            complement(1630..1938)
                     /locus_tag="PK590_05645"
     CDS             complement(1630..1938)
                     /locus_tag="PK590_05645"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOG24112.1"
                     /translation="MSSFKCKQCGSCCCQTGYVYLENGEAENLASHLGMELYDFTDRY
                     CDILEKKHLVLKGHADEACIFLDKNRCRVYAARPVQCRDFPLKWDTIERKNYCQGSKS
                     "
     gene            complement(1996..2340)
                     /locus_tag="PK590_05650"
     CDS             complement(1996..2340)
                     /locus_tag="PK590_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HOG24113.1"
                     /translation="MTEENRKTVLGVTENLEALICYALGWVTGLIFLLLEKNNEYVRF
                     HALQSLVTFGTLHAMSFVLMFIPILGWLVAFCLSALSLILWVVLMVKAYKGERYKLPV
                     AGDFVEQQMAKK"
     gene            complement(2540..3757)
                     /locus_tag="PK590_05655"
     CDS             complement(2540..3757)
                     /locus_tag="PK590_05655"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOG24114.1"
                     /translation="MRRNIEIVTNERCTGCAGCYNACPEGAIEMILSNEGFYRPIVDR
                     SRCTNCGFCIGKCPELSWETPLLSPPRVYAAWSRDDAVRLSSTSGGIFSELARGILDA
                     GGVVVGCAWNKNWEPEHFLIQSWDEVEKFRGSKYVPSKIGKAFRTIREISEKGREVLF
                     CGTPCQVAALGLSLSQKQREKVLLVDLICHGVPSLRAFQQYRDELFPEGLKSYWFRRK
                     SNDSSHNIRAVTQSERLYDGQFKNDPFMKAFLADHVIMMDSCHDCRYQGSIRPGDITV
                     GDFWGVPKQWSDWRGVSVILTNTLKGNRWIDLLQSKKNIELRETSFDIAASGNPKLLS
                     GVVPKPFWRAVFMRQLSQGVPFMKLFRYYSPVLKLYRQIAFSLTRWRQGRVLLKLKKL
                     ILKFFLKNKKDGL"
     gene            4049..4441
                     /locus_tag="PK590_05660"
     CDS             4049..4441
                     /locus_tag="PK590_05660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOG24115.1"
                     /translation="MATIFTKIISGELPAHKILEEDRFLAFLDIRPVNPGHTLVIPKQ
                     EIDYIFDLEDSLLKDLMVFAKKVSWGIRKAIPCKRIGVMVAGLEVPHTHIHLIPIVES
                     LHELNFSRAQSVPTEELARIAQKIRAHL"
BASE COUNT         1199 a         1263 c          896 g         1163 t
ORIGIN      
        1 cccttttgct ggatggcgta atcaaggatg gacatcaaat aaactttttg atccataaaa
       61 accttccttt ttgaaccgga gacccggcat tgtgttgggg cattttagca acctcgccgt
      121 gttgcatcaa gctggcgttc gttcccgccg gaaaaacttc ccgggaaact caaaaaaatc
      181 gctgttccat tttaattctt tcttgattat cataccagac catgaaggat gccaccgcat
      241 gagaattacg aaaaagactc ccgctcttga aatttatcag caggcttacc agctctacca
      301 gattcgcatt ctgacgattc ttaccctgat ccccctttat atcctgacga accggtgggg
      361 tcgttttgat gtcaatatct ggccgcttat tcttgtcatc ttgattgagg tgttcatcaa
      421 ccgtccctat cggtttttgt acaccgactc cgagtcgtct taccgggcca tggtcggttc
      481 cgtcatcatc gatttcgtgg ccgaaaccac ccttcttcac cttttgggcg acatcgattt
      541 ctttatttat ggcgtctgtt acttcattac catggtttat tgcgcgcttc gtttccccgc
      601 tttcatgacc ctcctttttg ccacgctggc atcgttcctc tacgtggggc tccttgccct
      661 gggccaagcc ggtcttatcc cgttaaccat ttcttttggc ccggaagtca cccggacgga
      721 acaagtcgga tttgtcgtcc ggcaaatcgc ctttttctat ctcatcggaa ttctcgtgcg
      781 attcgtggca aaggccatga cgcaaaaaga agaacatctt gaagagctca tttgggaact
      841 gaaatcaacc aatgaaaaaa taaaatacgc tcatcacctt cagacggatt atttcgccaa
      901 aatgagccat gaaattcgcg ctcctctcaa ttcgatcatg gggttttccc aattacttct
      961 tgagaacacg acgggacagc tcactgaaaa acagagggac tttatcgcgc ggattgaacg
     1021 aagcggtagc cacttgaggg atatgatcaa tgatgtcctg gacatttcca aaatcgaatc
     1081 aaaaaaaatg accctttcgc ttcgcgaggt caatctggtc aaagtcatcg attccgtttt
     1141 tgattttttc tacgaggaag cttccttaaa aaaaattctg ttcgccgtca ccgacaaacc
     1201 caaggaaccc ttgctcgtca cttgtgatga acttaaaatc cggcaaatac tctataacct
     1261 tctttcaaac gcgatcaaat tcactccctc ggggggccat attgccatgt ccctgaaaaa
     1321 agggccggaa ggcgccatca tctgcattga cgattcaggg ccgggaatta gcccggaatt
     1381 ccagaaagtg attttcacgc cttatgaaca aacgggacgt accacaaaca ccgaaagcaa
     1441 gggaacggga cttggcctgg cgatttcaaa acaatttgtg acaatgcacg gaggaaaaat
     1501 ttacgtgaaa agcgagccgg gacagggttc gcaattttac gtggaaattc ctttcagggc
     1561 gaaacctctc tctggtaaca aaccagccgc gtccggaaat ttcccagagc aattcggctc
     1621 catcaacccc taacttttag acccctggca gtagtttttt ctctcgatcg tatcccactt
     1681 caggggaaaa tcccggcact ggaccggtct tgccgcatag acccgacacc ggttcttatc
     1741 aagaaagatg caggcctcat cagcgtgtcc tttaaggaca aggtgctttt tctcaagaat
     1801 gtcacaatac cgatccgtaa aatcatataa ctccatccca agatgagacg ccagattctc
     1861 ggcctccccg ttttcgagat acacatatcc cgtctggcaa caacatgaac cacactgttt
     1921 acatttaaag gaagacatag aatggcgatg ccacatcccg cgaaaccgca gttcgcggac
     1981 cgtagctccg ggtagttatt ttttagccat ctgctgttcc acaaaatcac cggccacggg
     2041 cagtttataa cgttctccct tgtaagcctt aaccatcaaa acaacccaaa ggataaggct
     2101 cagagcgctg agacagaacg cgaccagcca gcccaaaatg ggaatgaaca taagcacaaa
     2161 tgacatcgcg tgcagggttc cgaaggtcac aagggattgc agggcgtgaa accttacgta
     2221 ttcattgttt ttttccagga gaaggaaaat caggccggtc acccatccaa gggcgtaaca
     2281 aatcaaggcc tccagattct ccgttactcc aagcactgtc ttcctgtttt cttcagtcat
     2341 ggttcacctc acttcattgt gttgataatt ctatcaaaaa taaacctaca actcgctatt
     2401 ctgttttccc caaacgctga aattaacacg tttcgtccct gcttttactc atacgtcact
     2461 ccctgcaacg cggtaaaatg accgatcgtt cggggtaagc ggatcgccac ccccgacccc
     2521 tcaaataact ttgccccgtc tacaaaccat cttttttatt tttcaaaaaa aactttaaga
     2581 tcaatttttt cagcttgagt aaaacccgcc cttgcctcca tcgggtcagg gaaaaagcta
     2641 tctgccgata cagcttcaga acaggcgaat aataacggaa taatttcata aatggcaccc
     2701 cttgggacaa ctgtcgcata aagacagccc tccaaaaagg tttcggtacc acaccggaca
     2761 aaagctttgg gttcccagac gccgcaatgt caaaactggt ttctcttaat tcgatgtttt
     2821 ttttactttg aagcaaatca atccatctat tccctttaag ggtattcgtc aaaataacag
     2881 atactccgcg ccagtcactc cattgcttgg gaacccccca aaaatctccc accgtgatat
     2941 ctcccggacg aatacttccc tgatatcgac aatcatgaca tgaatccatc ataatcacgt
     3001 gatccgccag gaaagctttc ataaacgggt cgttcttaaa ctggccatca tatagcctct
     3061 ctgattgagt cactgccctg atattatgac tgctgtcatt gcttttcctt ctaaaccaat
     3121 aggattttaa tccctcggga aacaactcat cacgatattg ctgaaaagcc cgcaaggatg
     3181 gcactccatg acaaatcaag tcgaccagaa gcactttttc tctctgtttt tgggataatg
     3241 aaagtccgag ggccgccacc tgacacggtg taccgcaaaa caaaacttcc ctgccttttt
     3301 cagaaatctc ccgtatcgtc cgaaacgcct tgccgatttt acttgggaca tatttactcc
     3361 cccgaaactt ctccacttca tcccaggatt gaatcagaaa atgttcgggc tcccaattct
     3421 tgttccaggc gcatcccacc acgacacctc ccgcatcaag aatccccctt gccaattccg
     3481 aaaaaatccc tcccgatgta ctggacaaac gcacggcatc atcccttgac caggccgcgt
     3541 acactcgcgg aggagataaa agcggagttt cccacgataa ttccggacat tttccgatac
     3601 aaaaaccaca attcgtacaa cgcgaccgat ctacgatggg acggtagaat ccttcgttcg
     3661 agagaatcat ttcaatggcg ccctcagggc acgcgttata acaaccagca caccctgtgc
     3721 atctctcgtt tgtcactatt tcaatattcc gacgcatcgc aactccccat caaccgtcac
     3781 cctgagacag gccagaaatc aacaccaccc gccagatcaa caagcctcga ttttcacgca
     3841 acagatgacc tcttttgaat tatcgagtct tcgatccgac gtttgagagt cagggttccc
     3901 gttctcgcaa atttatcttc gtaacactcc cgttgcctct gagatatctc gactttaccc
     3961 caccgtcgat tgatcttata atcgcggaag aaacacctgt cttcttccgc gccgtagcaa
     4021 caccacttgt caagaaatgg aggtcaacat ggcaacgatc tttacgaaaa tcatttcagg
     4081 agaacttccg gctcacaaaa tcctcgagga agatcgtttc ctggcgtttc ttgacatccg
     4141 cccggtcaat ccggggcata cgcttgtcat ccctaaacag gaaatcgact acatctttga
     4201 tctggaagat tcccttctta aagacctgat ggtttttgcc aaaaaagttt cctgggggat
     4261 ccgcaaagcc attccctgca agagaatcgg cgtgatggtt gctgggcttg aggttccgca
     4321 cacccacatc catctcatcc ccattgtcga gagccttcac gagctcaatt tttcccgcgc
     4381 ccaatccgtg cccacggaag aactcgcccg gatcgcccaa aaaatccgcg cccatcttta
     4441 ggtctggccg cgcgccccag actcccagcg gtcatggatt ggccgattct ttaggcaccc
     4501 agacaggcgc gaactttttc a
//
LOCUS       DAOSIO010000135         4493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_17099_length_4493_cov_4.395448, whole genome shotgun sequence.
ACCESSION   DAOSIO010000135 DAOSIO010000000
VERSION     DAOSIO010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17099_length_4493_cov_4.395448"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="PK590_05665"
     CDS             <1..155
                     /locus_tag="PK590_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOG24116.1"
                     /translation="IPFLVASFFGGTGLLIIVGVVLDVMRSIESQLIMRHYDGFIKKG
                     RIKGRR"
     gene            158..814
                     /locus_tag="PK590_05670"
     CDS             158..814
                     /locus_tag="PK590_05670"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013977739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOG24117.1"
                     /translation="MNLIFLGPPGAGKGTHAEVLSKTKNLCHLAAGDILRRNIKEGTS
                     LGMEAKDIMAKGELVPDMLVNSMMFAEIKMLKVKGCEGFILDGYPRTIGQAEELDKFL
                     ASEKMTLTAAINFDASEAVVVERLSGRRLCAVDGKIYHVKNMPPKVEGICDCHGQPLV
                     TRKDDQPETIRKRLAVYHESTKPLIEYYARKGILRNVQADGDAPLVQEKIAKLFEKVG
                     "
     gene            821..1579
                     /gene="map"
                     /locus_tag="PK590_05675"
     CDS             821..1579
                     /gene="map"
                     /locus_tag="PK590_05675"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOG24118.1"
                     /translation="MIELKSSREIQIIKESGKILQEVFKTLRPMIREKVTGIELDQAA
                     EKVIRESGAQPSFKGYRGYPASICVSLNEEVVHGIPGTQPLFNGDIVSIDVGVKWNGF
                     HTDAARTWAVGAVDDRRKKLIATAKDSFDVALSKIKVGWRIGDLSEAIQKYVESQGFQ
                     VVRDYAGHGIGRELHEDPQVPNYGKAGRGAKIEPGLVIAIEPMVTEGSYAVETLSNGW
                     TVVTKDRKCASHYENTVAFTEGGFINITKDEATE"
     gene            1579..1797
                     /gene="infA"
                     /locus_tag="PK590_05680"
     CDS             1579..1797
                     /gene="infA"
                     /locus_tag="PK590_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOG24119.1"
                     /translation="MPKEDAIEVEGTVIEPLPNAMFRVELENGHKVLAHISGKMRMHY
                     IRILPGDRVKVELSPYDLTRGRITFRVK"
     gene            1814..1927
                     /gene="rpmJ"
                     /locus_tag="PK590_05685"
     CDS             1814..1927
                     /gene="rpmJ"
                     /locus_tag="PK590_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012626782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOG24120.1"
                     /translation="MKVRSSIKRICEKCKIVRRKGYVYVICSNPKHKQRQG"
     gene            1986..2372
                     /gene="rpsM"
                     /locus_tag="PK590_05690"
     CDS             1986..2372
                     /gene="rpsM"
                     /locus_tag="PK590_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOG24121.1"
                     /translation="MPRIFGVDIPREKKVEIALTYLYGIGRARSRQVLDEARVDWNKR
                     ANDLTESEISAITGIIQKTCRVEGDLRREVQMNIKRLMDIRCYRGVRHVKGLPVRGQR
                     THTNSRTRKGKRKTVGGLSKKAPAPK"
     gene            2529..2900
                     /gene="rpsK"
                     /locus_tag="PK590_05695"
     CDS             2529..2900
                     /gene="rpsK"
                     /locus_tag="PK590_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOG24122.1"
                     /translation="MAKKKKVKHLARGVVHIAASFNNTIITISDAGGNSLVWESAGSA
                     GFKGSKKSTPFAAQIASEKAAKKAMEYGMKEVEVYIKGPGAGRESAIRALQAAGLSVT
                     AIHDITPVPHNGCRPPKKRRV"
     gene            2904..3527
                     /gene="rpsD"
                     /locus_tag="PK590_05700"
     CDS             2904..3527
                     /gene="rpsD"
                     /locus_tag="PK590_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOG24123.1"
                     /translation="MGRYRGPVIKLHRREGVNLGLKGRRATDEKHDKRLTNPPGQHGA
                     RRSKISDYGVQLREKQKMKRIYGLGERQFRVFFYRAAQKKGVTGETLIESLERRLDNV
                     VYRSLFCTSRREARQMVTHGLITVNGQKVDVPSYLVKVGDKIAPKAKESTQKRVQASL
                     EKWKDLTTPEWIGLDREKMISEVLRFPKKSDAGLPVEESLIVELYSK"
     gene            3663..>4493
                     /locus_tag="PK590_05705"
     CDS             3663..>4493
                     /locus_tag="PK590_05705"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOG24124.1"
                     /translation="MGIRWKTFEMPKRLEVDKNTLTATYAKFVAEPFERGYGTTIGNA
                     LRRVLISSIEGATVTSIKIDGMLHEFATLDGVVEDGAQIVLNVKKLVLRYHGKGPKNI
                     YINVKKQGPVKASDIEADETVEIVNPDLVICTLSKAVPFKMEMEIGRGRGYVPAEKNK
                     QDETTIGVVSVDSVFSPVTKVNFTVEDTRVGQMTDYDRLILEVWTNGAMGPEEALLYA
                     SNILQRHLDIFVNYGELPEEEEEEEETDHGFMEVINKPISELELSVRSANCLEAANIK
                     T"
BASE COUNT         1222 a          908 c         1307 g         1056 t
ORIGIN      
        1 atattccatt tttggtcgcc agtttttttg gcggtaccgg tttgttgatc atcgtgggtg
       61 ttgtgttgga tgtgatgcgc tccatcgaat cccagctcat tatgcggcat tatgacggct
      121 ttattaaaaa aggccggatt aaggggcgga ggtaagcatg aacctgattt ttttagggcc
      181 tcccggagcg ggaaaaggaa cgcatgccga agtcctgagt aagacgaaaa atctttgcca
      241 tctcgcggcg ggagatatcc ttcgtcggaa cataaaagaa ggcacgtcgc ttggcatgga
      301 agccaaggat attatggcta agggtgagct ggttccggat atgcttgtaa atagcatgat
      361 gtttgcggaa atcaagatgc ttaaagtgaa agggtgtgaa ggttttatcc ttgacggata
      421 tccaaggacg atagggcagg ctgaagaact cgataagttt ctcgcttcgg aaaagatgac
      481 gctgaccgcg gcaatcaatt ttgacgcgtc ggaagcggtg gtagtcgaac gtctttccgg
      541 ccgcagatta tgcgcggttg atggaaaaat atatcacgtg aaaaatatgc cgcctaaagt
      601 cgaaggaatt tgtgattgtc acggacaacc tcttgtgact cgaaaagatg accaaccgga
      661 gactattcgc aaacgtctcg cggtttacca tgagtcgacg aaaccgttaa ttgaatatta
      721 cgcccggaaa gggattcttc gtaatgttca ggcggacgga gacgccccat tggttcaaga
      781 gaaaatagca aagctttttg agaaagttgg ctgatgcggc atgattgaac tgaaatcaag
      841 ccgcgaaata cagattatca aagaatcggg gaagatttta caggaggttt tcaaaaccct
      901 tcgcccgatg atccgtgaga aagtaacagg gatcgaactg gatcaggccg cggaaaaagt
      961 gatacgagaa agcggtgccc agccgtcttt taaagggtat cgggggtatc cggcgtcgat
     1021 ttgtgtctca cttaatgaag aggtggttca cgggataccg ggcacccagc cgctttttaa
     1081 tggagacatc gtcagtattg atgtgggcgt aaaatggaac ggttttcaca cagacgcggc
     1141 aagaacctgg gcggttggag cggttgatga tcgacgaaag aaactgattg ctaccgctaa
     1201 agattccttt gatgtcgctt tgtccaagat taaagtgggc tggcgaatag gggatctttc
     1261 agaggcgatt caaaagtatg tagagtcgca aggattccag gttgtccggg attatgccgg
     1321 acacggcatt ggtcgggagt tgcatgaaga tccgcaggtt cccaattacg ggaaggcggg
     1381 gcgtggcgcg aagatcgagc cggggcttgt aattgcgatt gaaccgatgg tcacggaagg
     1441 cagttacgcg gttgagactt tatcgaatgg ctggacagtg gtgactaaag accggaagtg
     1501 cgcgagtcat tacgaaaata cggtcgcgtt tacggaaggt ggttttatta atattactaa
     1561 agacgaggca accgaataat gccgaaggaa gatgcgattg aggtagaagg aactgtgatt
     1621 gagcctctcc ccaacgcgat gtttcgagtt gaacttgaaa atgggcacaa agtgctggct
     1681 catatttcgg gtaaaatgag gatgcattat atccgtattc ttccgggaga cagggtgaag
     1741 gtggagcttt cgccttatga cctgacgcgt ggccgaatta cgtttcgggt taaatgaccg
     1801 ttaaggagaa actatgaaag tcagaagttc aataaaaaga atttgtgaaa aatgtaagat
     1861 cgtacgccgg aaaggctacg tctacgtgat atgttctaat ccgaagcaca aacagagaca
     1921 aggctgagca gacgcgcgtc tgtttttaaa attcgaatca cttgctcagg aaaggaaacc
     1981 aagttatgcc gcgtattttt ggtgtggata tcccaagaga aaaaaaagta gaaattgccc
     2041 tgacgtactt gtacggaatc ggtagggcgc gttcacgaca ggtgctggat gaggcccgtg
     2101 tcgactggaa caaacgcgcc aatgatctga ccgagagcga gatttcggcg attaccggta
     2161 ttattcagaa gacttgccgc gtggaaggtg atttgcgccg agaggtacag atgaatatca
     2221 agcggttgat ggatattcgt tgctatcgtg gagttcggca tgttaaaggc cttccggtgc
     2281 gtggacaaag gacgcatacc aattcacgta cgcgcaaggg gaaaagaaaa acggtggggg
     2341 gacttagtaa gaaggccccg gctcccaaat aaagcgcgac tgattttcgc tgaccgggcg
     2401 gtttgggatg attttttggc agggcgactt ggagctccgg ttggtgtgaa taacgagaaa
     2461 gataaatttt ccgattgcgt tgcttctcaa agcaatgtta cgggttcatt ggaaatgagg
     2521 aaaccataat ggcaaagaaa aaaaaggtca aacatctggc tcgcggtgtg gttcatatcg
     2581 cggcgagttt taataatacg atcattacca tctcggatgc tggcggcaat tccctggttt
     2641 gggaatcagc ggggtctgcc ggtttcaagg gatccaagaa gtccactccg ttcgcggccc
     2701 agatcgcgtc cgaaaaagcc gcaaaaaagg cgatggagta tggaatgaaa gaggttgagg
     2761 tttatatcaa agggccgggt gccggtcgcg aatccgccat ccgagctctt caggccgctg
     2821 gactttcggt gacggcgatc cacgacatta ctccggttcc gcacaacgga tgtcgtccgc
     2881 cgaaaaagag gagggtttaa gttatgggaa ggtatcgcgg tccagttatt aaacttcatc
     2941 ggcgtgaggg agtgaacctt gggcttaaag gccgtcgcgc cacggatgaa aagcatgaca
     3001 agcgcctgac caatcctccc ggtcaacatg gcgcccgacg ttctaagatc tcggattacg
     3061 gagttcagct acgtgagaag caaaaaatga agagaatcta cgggttgggt gagcgccagt
     3121 ttcgcgtctt tttttatcgg gccgcccaaa agaaaggggt tacaggtgag acgctgatag
     3181 aatctctgga gcggcgtctt gataacgtcg tctatcggtc acttttttgc acgtcgcgcc
     3241 gtgaagcccg acaaatggtg acccatggtc ttatcactgt gaacggtcag aaagtggatg
     3301 tcccttctta tctcgtcaaa gtaggtgaca agatcgctcc caaggcgaag gaatcgactc
     3361 aaaaaagggt gcaggcgagc ttggagaagt ggaaagattt aaccacgccc gaatggatcg
     3421 gtcttgacag agagaaaatg atcagtgagg tattgaggtt cccgaaaaaa tcggacgcgg
     3481 gattgccggt cgaagaatcg ctgatcgtgg agttgtattc caagtagtct ttttatccgc
     3541 ttcgccggtt tcgaacaggc agaatggaga acgggcgagg ggatcttgat aaaaattaaa
     3601 tgattcgtga tcaaacggat caattttgcc ccgcagcgcg ggagtcatcg gaggaaaata
     3661 aaatgggaat acgctggaaa acctttgaaa tgccaaagcg cctggaagtg gacaagaaca
     3721 cgttgaccgc cacttatgcg aaatttgttg ccgagccctt tgagcggggg tacggaacga
     3781 cgatcggaaa tgcccttcgg cgggttttga tttcgtcgat tgagggtgcc actgtgacca
     3841 gcatcaagat cgacgggatg ctccatgagt ttgcgaccct ggacggtgtg gtcgaagatg
     3901 gcgcgcagat cgttttgaat gtcaaaaaac tggtgctccg ctatcacggc aaggggccta
     3961 aaaatattta catcaatgta aaaaaacagg gaccggtcaa ggcctctgac attgaggcgg
     4021 atgagaccgt ggaaattgtc aatccggatc ttgtgatttg cacactttct aaagcggttc
     4081 cgttcaagat ggaaatggaa attggcaggg ggcgtgggta cgtgcctgcc gagaagaaca
     4141 aacaggatga gacgaccatt ggcgtggtga gcgtcgattc tgttttttct cccgtgacca
     4201 aggtgaattt taccgtagaa gatacccggg tcggacaaat gaccgattat gatcgcctta
     4261 tccttgaagt ctggacgaac ggcgccatgg gccccgagga ggcgttgctt tacgcatcca
     4321 atattcttca gcgccatttg gatatctttg ttaattatgg cgagcttccc gaagaggaag
     4381 aggaagaaga agaaacggat cacggattta tggaagttat taataagcct atcagcgaac
     4441 tggagctttc cgtgcgcagt gctaactgcc tcgaggccgc caacattaag act
//
LOCUS       DAOSIO010000136         4481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_17163_length_4481_cov_4.203570, whole genome shotgun sequence.
ACCESSION   DAOSIO010000136 DAOSIO010000000
VERSION     DAOSIO010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17163_length_4481_cov_4.203570"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PK590_05710"
     CDS             complement(<1..534)
                     /locus_tag="PK590_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOG24125.1"
                     /translation="MGRKKGKFILGIDLGGTKILMGLLDRQFCLKKTVKLEMDGNRGE
                     KFFFNKLIDGIEEVLEETGSDLSDLLTIGIGCPGIIDTHKGIVKVSSNVSFFKNYPLA
                     EKLKSKLKVPVLVENDANAALFGEQQFGAAHGYREVVGAFLGTGIGGALIINGYLYRG
                     VSGGAGEIGQMFFTMPSC"
     gene            complement(665..850)
                     /locus_tag="PK590_05715"
     CDS             complement(665..850)
                     /locus_tag="PK590_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24126.1"
                     /translation="MPARLKNTLIHTESCAIWSAFDQESKEIPYVLRAGRHVDSSREI
                     SDGFLDSRLKDSLKMKL"
     gene            complement(867..1094)
                     /locus_tag="PK590_05720"
     CDS             complement(867..1094)
                     /locus_tag="PK590_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24127.1"
                     /translation="METKEALIVAVRSQRDLLNGLVEQLEMKEVLTMEDYSYCESCAG
                     EVAQKMKEIRRAALDKMIQGVSLAKILGVAR"
     gene            complement(1142..>4481)
                     /locus_tag="PK590_05725"
     CDS             complement(1142..>4481)
                     /locus_tag="PK590_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24128.1"
                     /translation="DIFNESYNLAHHLGAMVAVLDHYVKKLIPGLGPERRKLSFRSWR
                     MIIHNRLVRFFFPVARLWYLISPVAAIHRIITFRSFRSLNRKMVKLGLTTPADAQKVF
                     EDARAAIKSAFYGQVTLDAAVGLVERHARFWRRALAMTGISALFLPMVILIYLGEHWL
                     NATLMMMMGFGTVMLPLAAIACQVGPVLIRFVGPYYRIWERELGRLKKQNRRLERSLP
                     EIPKPKPVPQNPVPRETSQRGSEQIAMSKVLRALHDTTLMPDVEVIGLVTPWYGENKA
                     ALEQLIREDAILKQWAITVEESDGEFPGDAGLFFAARNIAKTSQKKFKIYWYPQAGMD
                     FQTAVAVFKINLGEAARMIGELRAVGVQYGEIAFPGGDLVSSSSAITRNGGHFVVQTR
                     YASLDEVESERLPVILGRNGTSRLEIEHIVYQPQKLREMMSAGDLHLGSGRVSISSAA
                     VPQFPVPTGPVAIVAETSEDHQAVQSLLQSTRDRILNLTDRYGPFRINFLEDIVRPVA
                     ALSRQIEGGNGVTPPNPNLLVGRTVRLSDDTVISPYDKLYTTVSRFLWNGGNGDRRSF
                     KSEAFIPPLSETFYVSGKTREKLAETLDEISANFPEFVNPELTQRLRQETPAVSKGRF
                     VFRDDAAQIVYEEKETGYKRGLRSEDVAGLVIRDQMIALQRRDHQIGNEPGRLDVSFA
                     GQQDETDQGPEATASREFREELLGGRRPVPEGVVFRPIGSPVQLEITHESEQGLSVRK
                     KNTTVYGVLFKNEFSRDDFLPGGNAAEIVWMTKEDLEKELKQTPGRFTARAFQLFTKL
                     GSTVAFSEGGVRSEVRVARTGAGIHMPPAVISLPRAPAVLFQDDQEISPAPQMPEQIR
                     ILTAILVAQKILAARDAELLPELEASLRDAARIAERVAPALSSLSGAHDVPIKELKAG
                     LEALLRQEQSEILADDRIVFLDAGTISEEYFDRIVSSPFPVVILFDRDHKELYDRLTK
                     ESSRATNAFLIYTPDGINSFIRNSLRSGAVAVTASPRLNGILARFREQGKSEDIALVA
                     SSRVESQKIEQDYVGHRYYFTDEMLHIDPELVAYSLVLLLRSPELFRYGKGRYAQDVV
                     SLIGLVMQQLAEEFQIRLKTGSAA"
BASE COUNT         1115 a         1266 c         1030 g         1070 t
ORIGIN      
        1 acaagaaggc atggtgaaga acatttgacc aatctcaccg gcacctcccg aaacaccgcg
       61 gtaaagatac ccattgatga taagcgcccc accgattccc gtaccgagga aagccccgac
      121 cacttcacga tacccgtgtg ccgcgccgaa ttgttgttcc ccgaacaaag cggcattggc
      181 atcgttttcg acaagaaccg gcacctttaa ttttgatttt aatttctccg ccaaaggata
      241 atttttaaag aaactgacat tcgaagaaac tttgacgatt cctttatggg tatcgatgat
      301 cccgggacag ccgatgccga tcgtcaaaag atcactcaaa tccgaaccgg tttcctcaag
      361 gacctcctcg atcccgtcaa tcagtttgtt gaaaaaaaac ttttccccgc ggttgccgtc
      421 catttcaagc ttgacggttt tttttaaaca aaattgccga tccaaaagac ccattaagat
      481 ctttgtcccg cccaaatcga ttccgagaat gaacttccct ttcttcctcc ccatagtccc
      541 cccgttctct tggttaattg ttgctattat agtggattat ggggatactt atgtaaaaga
      601 ttagtaaaat cccggaaaac cgttgtcttg tcgaaggggg aaaatccctg ccagaggtag
      661 gaaattacaa cttcattttg agcgagtctt tcaaccgact gtccagaaat ccatccgaaa
      721 tttccctgga tgaatccaca tgccttcccg cgcgtaaaac ataagggatt tctttggact
      781 cctgatcaaa cgcgctccat atcgcacaag attccgtgtg aatcagtgta tttttcaatc
      841 gagccggcat tttcacgctt caggccctaa cgggcaacgc caagaatctt ggctagggaa
      901 acaccctgga tcattttgtc cagagcggct cgccggattt ctttcatttt ttgggcgacc
      961 tcacccgcgc aactctcaca ataggaataa tcctccattg taaggacctc tttcatttcg
     1021 agttgctcca ctaatccatt gagaagatcc cgctgggacc tcaccgctac gattaacgcc
     1081 tcctttgttt ccatacctgc actccttttc ttcttgatct ggtttaaaaa tatcctcaaa
     1141 attacgcggc gctaccagtt ttaagccgaa tctggaattc ctccgcaagt tgctgcatca
     1201 caagcccgat caaagacaca acatcctggg cataccggcc ttttccgtag cggaaaagct
     1261 ccgggcttcg caaaagtaac acaagcgaat aagcgacaag ctccggatca atatgaagca
     1321 tctcgtccgt aaagtaatac cggtgcccga cataatcctg ttcgattttc tggctttcga
     1381 cgcggctact tgcgaccagg gcaatgtcct cggattttcc ttgttcccgg aaacgggcta
     1441 aaatgccgtt aagtcgcggc gaagccgtga ccgctactgc cccggaacga agggagttcc
     1501 tgataaaact gttgatgcca tcgggcgtat aaatcaaaaa agcattcgtg gcacgagacg
     1561 actctttagt caggcggtca taaagctctt tgtgatcacg atcaaacaag atgacgacgg
     1621 gaaacggtga actcacgatc ctgtcgaaat attcctccga aattgtcccc gcatccagaa
     1681 agacgatgcg atcgtccgcg aggatctcgc tttgttcctg acggagcaac gcctccaatc
     1741 cagctttcaa ctccttgatc gggacatcat gagcgccaga cagagaggaa agtgcgggag
     1801 cgacacgttc cgcaatcctg gcggcatccc gcaaagaggc ctccagttcc ggcaaaagct
     1861 ccgcgtcacg tgccgcgaga attttttgag ccacaagaat agccgtcagg atacggattt
     1921 gttcgggcat ctggggcgcg ggactgatct cttgatcatc ctgaaacaac accgctggcg
     1981 cgcggggcaa cgaaatgaca gccggcggca tatgaatgcc ggcccccgtg cgcgcgacac
     2041 ggacttccga tctgactccc ccttcagaaa aagcaactgt ggaaccaagt ttggtaaaca
     2101 attgaaaagc gcgggcggta aagcggcccg gagtttgctt caattctttc tccaaatctt
     2161 ccttggtcat ccaaacaatc tcagccgcat tgcccccggg aagaaaatca tcccgggaaa
     2221 actcgttttt gaataaaacc ccgtaaactg tcgtgttctt tttacgaacg gacaggcctt
     2281 gctcgctttc gtgcgtgatc tccagctgaa ccggagaccc gatcgggcga aagaccactc
     2341 cctcaggaac tggacgtcgt ccgcccaaaa gttcttctcg aaattcacgc gatgccgtgg
     2401 cctccgggcc ttgatcggtt tcatcctgct gtccggcaaa tgatacgtcc aggcggccgg
     2461 gctcgttccc gatctgatga tcccgccgct gtaaggcaat catctgatcc ctgatcacaa
     2521 gtcccgcaac gtcttcggac cgcaatccgc gcttatatcc cgtttccttt tcttcataaa
     2581 caatttgagc ggcgtcatcc ctaaaaacga accttccttt tgatactgcg ggtgtttctt
     2641 gccgcagtct ttgggtaagc tcagggttta caaattccgg aaaattggca gagatttcat
     2701 ccagagtttc cgcgagtttt tcgcgggtct taccagaaac ataaaatgtc tccgaaaggg
     2761 gggggatgaa cgcttcggac ttgaaactcc gccgatctcc attgccgccg ttccacagaa
     2821 aacggcttac ggtcgtataa agtttgtcgt agggactgat gaccgtgtcg tcggaaagac
     2881 ggacggtccg tccaaccaat aaattgggat tgggaggagt gacaccgttc cccccttcta
     2941 tttgccggga taacgccgcc acaggacgca cgatgtcttc aagaaaatta atccggaaag
     3001 ggccgtaccg gtccgtcaag ttaagaattc tgtcccgtgt gctttgcaaa agactctgga
     3061 ccgcctggtg gtcctccgac gtctctgcca cgatcgcaac ggggcctgtc ggcacgggaa
     3121 actgtggcac agcggcgctt gatatgctca cacgcccgga acccaagtgc agatccccgg
     3181 cagacatcat ttctcttagc ttttgaggtt gatagacaat gtgttcgatc tccaagcggc
     3241 tggttccatt gcgtcccaaa ataaccggca atctttcact ttcaacctca tcgagactgg
     3301 catatctggt ttgaactaca aaatgtccgc cgttcctggt aatcgccgaa gaactgctga
     3361 ccagatcccc tcccggaaac gcgatttcac catattgaac accgacagct ctcaattcgc
     3421 cgatcatcct ggccgcttcc cccagattga tcttaaaaac cgcaactgcc gtctggaaat
     3481 ccatgcccgc ctggggatac caatagatct tgaacttttt ttgactcgtc tttgcgatat
     3541 tccgcgccgc aaaaaacaat ccggcatccc cgggaaactc tccgtccgac tcctcaaccg
     3601 tgatagccca ttgcttcaaa atcgcatctt cccggattaa ctgctccagg gcggccttgt
     3661 tctcaccata ccagggcgta acaagcccga tcacctccac atcaggcatt agagtggtat
     3721 cgtgcaaggc tcgcagaacc tttgacatcg cgatttgttc gctaccccgt tgcgacgtct
     3781 cacgaggcac cggattctgc ggaacaggtt tcggtttggg tatttcaggt agcgagcgtt
     3841 ccaaacggcg attttgtttt ttcaagcgcc ccagttcacg ctcccaaatt cgataataag
     3901 gtccgacaaa ccgtatgagg acgggaccga cctgacacgc gatcgcggcc agcggaagca
     3961 tgaccgttcc gaaccccatc atcatcatta aggtcgcgtt cagccaatgc tcccccaagt
     4021 aaatcaggat caccatcggg aggaagagcg cggaaatgcc ggtcatcgcc aaagcacgcc
     4081 tccagaacct cgcatgcctt tcgaccagac cgactgcggc atccagggtt acctggccat
     4141 aaaacgcgga tttgatcgcc gctctggcat cttcgaaaac cttctgcgcg tccgcgggtg
     4201 ttgtcaaacc cagttttacc atttttctgt tgagggaacg gaaactccgg aaagtaatga
     4261 tccggtgaat agcggccacg ggacttatca aataccagag gcgagcaaca ggaaaaaaga
     4321 atcttaccaa ccggttatgg atgatcatcc gccagctacg gaacgaaagc tttctgcgtt
     4381 cgggcccgag tcctggaatc agttttttta cataatggtc caataccgcc accatggccc
     4441 cgaggtgatg cgcaaggtta tagctttcat tgaaaatatc c
//
LOCUS       DAOSIO010000137         4453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_17284_length_4453_cov_3.457935, whole genome shotgun sequence.
ACCESSION   DAOSIO010000137 DAOSIO010000000
VERSION     DAOSIO010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17284_length_4453_cov_3.457935"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..660)
                     /locus_tag="PK590_05730"
     CDS             complement(25..660)
                     /locus_tag="PK590_05730"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOG24129.1"
                     /translation="MQTELFDINSQSQTCTESETPGAKQHLLERTFLSLRLDQTIGIV
                     LVLMVFYAVVFSLGFEKGKRLIRHDQVIKAVNPKPVDQSLPPISKNLILYEEPQTELV
                     ETAVVATVPQPAPSAVTATSTSENRSISSARPDGKYTIQHVIYLTRSAAEKEVQKLTQ
                     KGEKAFIVPSGKYMQVCLSGFPSKKEADQYLRQLRSQNIIASDAYVRNMPA"
     gene            complement(780..1421)
                     /gene="lepB"
                     /locus_tag="PK590_05735"
     CDS             complement(780..1421)
                     /gene="lepB"
                     /locus_tag="PK590_05735"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOG24130.1"
                     /translation="MKIWIFWSIVFAVALFVYREKIARDIVFARTDFKKWAYLKWKNW
                     GEPLLIAAILAIAIRTFIIGPYKIPSGSMKPTFIEEDKIFVDKISYRFHEPARGDVIV
                     FKYPLDRKKDFVKRLVGLPGETIEIRDGVILVDGKPMTRPPFSKNHYYNVEDWEYGAS
                     GRIIKVPDGHYFTLGDNSAHSADSRQWGFVPRKDVIGKASVIWWPPKRIRLVK"
     gene            complement(1425..3206)
                     /gene="lepA"
                     /locus_tag="PK590_05740"
     CDS             complement(1425..3206)
                     /gene="lepA"
                     /locus_tag="PK590_05740"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOG24131.1"
                     /translation="MLPSKIRNFSIIAHIDHGKSTLADRLLLNSGAITAREFREQILD
                     DMDLERERGITIKARAVRIHYKDHWLNLIDTPGHIDFTYEVSKSLAACEGVLLLVDAG
                     QGIQAQTVTNLYLAMERNLTIIPVVTKIDLPTAEPEKVRKEVAQFLNVDEQTVILSSA
                     KSGEGAESILDAIIDRIPAPQGDPEKPLKALIFDSKYDTYQGVITYFRVMEGTLRTHD
                     QVLMMQTGTQQEALEIGIFSPKPEAVEALGVGAVGYMTANIKEVAEVKIGDTLTLAAA
                     PALEPLPGYQEIKPMVFCGLYPINAKDFPLLRVALSKLRLNDSSFVYEPETSASLGFG
                     YRCGFLGLLHMDIVKERLEREFNLELIITAPSVVYQILTKKGEIIELDNPTKLPHPTM
                     METIDEPYILARLMVPTDTLGAVMQLCQDRRGIYKSTEYLDPTRAVLTYEIPFSEVVM
                     DFYDKIKSMTAGYGSLNYEFIGHKPSELVKLDVLINGEPVDALSSITCKEKAYQRGRS
                     LVEKLKEVIPRQMFEVVIQAAVGSKIIARDSISAMKKNVTAKCYGGDITRKRKLWERQ
                     KEGKKRMKKIGRVDLPQEAFISVLKVE"
     gene            complement(3206..3859)
                     /gene="rpe"
                     /locus_tag="PK590_05745"
     CDS             complement(3206..3859)
                     /gene="rpe"
                     /locus_tag="PK590_05745"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOG24132.1"
                     /translation="MRRVLIAPSLLSADFSRLAEEIRDVERAGCDVLHVDVMDGHFVP
                     NLTIGPLVVKAIRKVTSLPLDVHLMIDQPIRYIADFRKAGADWITIHVEAEKDVGKCL
                     AEIKSLGARAGISLKPGTSAESLTPYWADLDLVLVMTVEPGFGGQAFMPAQVSKIRVC
                     RQQFKGLISVDGGIDPQVAPQVIEAGADVLVAGSAIFGKPDRGKVIAEFKEAAKKSL"
     gene            complement(3856..>4453)
                     /locus_tag="PK590_05750"
     CDS             complement(3856..>4453)
                     /locus_tag="PK590_05750"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOG24133.1"
                     /translation="PRICFHEKKTEQTHFVIGFHALARMHPDRYKLGLLNIILGANMS
                     SRLFDEVREKRGLAYEIKSGVSAYADAGAFTISAGVETVKTELAVRVILKELDKVTRK
                     MVTPGELRRAKDYFMSQVYLGVEDTLDHLLWAGEKLLCANELPNKEDIRRKIEEVTQE
                     EIREVARRLFRTQRLNLAVVGPVNEPMQKAIRKGLVIE"
BASE COUNT         1105 a         1216 c         1024 g         1108 t
ORIGIN      
        1 tgtttttacc tcaaaagcat aagatcaagc gggcatgttg cgaacgtagg cgtcacttgc
       61 gatgatgttc tgggatcgca gttgccgcaa atactgatcg gcctcttttt tggagggaaa
      121 accggaaagg caaacctgca tgtacttgcc actcgggaca ataaacgcct tctctccctt
      181 ttgggtaagc ttttgcactt ccttctcggc cgccgaacgc gtcaggtaga ttacatgttg
      241 aatggtgtat ttaccatccg gccgagcgga cgagatggac cgattttccg aagtagaagt
      301 cgcggtaacg gctgaaggag ccggttgagg cacggtcgcc accacggcgg tttcaactaa
      361 ttccgtctgc ggctcctcat aaagaataag attcttggaa attgggggga gagattggtc
      421 gaccggttta gggttaacag ctttaatcac ctgatcatgc cggatcaagc gttttccctt
      481 ctcaaaaccc aacgaaaaaa caacggcata aaaaaccatc agaactaaaa caatcccgat
      541 cgtctgatcc agccgaagtg acaaaaaagt ccgttcgaga agatgctgtt tggctccggg
      601 cgtttctgat tcagtgcaag tctgggactg cgagttgatg tcaaataatt ccgtttgcat
      661 aaaaaattcc tccgcggagg atttaacggc aaacaggcgt caagacttga tgagattttg
      721 gaaaaacaat ggggtggtgt ttgaacaatg gataaaaaaa atccgccttg cggtccggct
      781 tacttgacaa gcctgattct cttcggcggc caccaaatca ctgaagcttt gccaatcaca
      841 tccttgcggg gtacaaatcc ccactgacgg ctgtccgccg aatgcgcgga attatctccc
      901 aacgtaaaat aatgaccgtc cggaaccttg ataatacgtc ccgacgcgcc atactcccag
      961 tcttcaacgt tgtagtaatg atttttgcta aaaggcggcc gggtcattgg ttttccatcc
     1021 accagaataa ccccgtcacg gatttctatc gtctccccgg gaagcccgac aagtcgttta
     1081 acaaagtcct ttttccggtc aagcggatac ttgaacacaa tcacatcccc tcgcgctggt
     1141 tcgtggaaac gataagaaat tttatccaca aaaatcttgt cttcttcgat aaacgtcggc
     1201 ttcatagaac cggaagggat tttgtaaggg ccaataataa aggtccggat cgcgatcgca
     1261 aggatcgcgg caatcaggag cggttctccc caatttttcc atttgagata cgcccacttt
     1321 ttaaaatccg ttcgggcgaa cacaatatcc cgcgcgattt tttcgcgata cacaaacaag
     1381 gctacggcaa atacgatgct ccagaaaatc cagattttca tagtctactc cacttttaaa
     1441 acgctgatga aggcttcctg cgggagatcc actctcccga ttttcttcat ccgttttttc
     1501 ccttcctttt gacgttccca gagctttcgc ttccgggtaa tgtctccccc gtaacatttg
     1561 gcggttacgt tctttttcat cgcgctgata gagtcccgcg caatgatctt ggaaccgacc
     1621 gcggcctgga tgacaacctc gaacatttgc cttgggatca cttctttgag tttttccaca
     1681 agagaccgcc cccgttgata agccttctcc ttgcatgtga tggacgacag ggcgtccacc
     1741 ggctctccgt taatcaaaac gtcaagtttc acgagttcgg acggcttgtg cccgataaat
     1801 tcataattca acgatccgta tccggctgtc atggacttaa ttttgtcgta aaaatccatc
     1861 accacctcgg aaaagggaat ctcgtaggtc aaaacagctc tcgttggatc cagatactcc
     1921 gtgcttttgt aaatcccgcg ccggtcctgg caaagttgca tcaccgcgcc caaagtgtcc
     1981 gtcggcacca taaggcgcgc gagaatgtaa ggttcatcga tcgtctccat catcgtcgga
     2041 tgcggaagtt tggtcgggtt atcaagttcg ataatctccc cctttttcgt caggatctga
     2101 tacaccacgc tcggagctgt aataatgagc tcaaggttga actcacgttc gaggcgttcc
     2161 ttgacaatgt ccatgtgtaa aagccccaaa aaaccacacc ggtatccaaa ccccagagag
     2221 gccgaagtct cgggctcata aacaaatgaa gaatcgttca atcggagttt cgatagcgca
     2281 acacgcaaca agggaaagtc cttcgcgtta atcggataaa gtccgcaaaa aaccatcggc
     2341 ttgatttcct gataaccggg aagaggctcc agcgccggag ccgccgccag ggtcagcgta
     2401 tcgccgattt tcacttcggc cacttccttg atgttggcag tcatgtagcc aaccgcaccg
     2461 acccccaatg cttccacggc ttcaggtttt ggactaaaaa tcccgatttc cagcgcttcc
     2521 tgctgggtcc cggtttgcat cataagcacc tggtcatggg tgcggagcgt accttccatg
     2581 acccgaaaat acgtaatcac cccctgatag gtgtcatatt tggaatcaaa aataagcgcc
     2641 ttgagtggct tctccggatc tccctggggc gcgggaatcc ggtcgatgat tgcgtctaaa
     2701 attgattccg ccccctcccc gctcttcgcg ctggaaagaa tcaccgtttg ctcgtcaacg
     2761 tttaaaaatt gcgcgacttc tttcctgact ttttcaggtt ccgcggtcgg aagatcgatc
     2821 ttggtcacca ccggaataat ggtcagattg cgttccatgg cgagataaag attagtcacg
     2881 gtctgagctt gaatcccttg tcccgcgtca accaacaaca aaacaccctc gcaggccgcg
     2941 agactctttg aaacttcgta agtaaaatca atgtgcccag gtgtatcaat gagattaagc
     3001 cagtggtcct tgtagtgaat gcgaacggcg cgagccttga tcgtaatccc ccgctcccgc
     3061 tcaaggtcca tatcatccag aatttgttcg cgaaattccc gtgctgtgat cgctccgcta
     3121 ttgagcaaga gacgatccgc aagagtcgat ttgccgtgat caatatgcgc gataatggag
     3181 aaatttctta tcttcgaggg taacattaca aagatttctt ggcggcttct ttaaattcgg
     3241 caatcacctt gccacggtcc ggctttccaa aaatggcact cccggcgacc aaaacatccg
     3301 cgccagcttc gataacctgg ggagccacct gcgggtcaat cccgccatca acggaaatca
     3361 gtcccttaaa ttgttgccgg caaaccctga ttttggaaac ctgggctggc ataaacgcct
     3421 gtcctccgaa gccgggctcc actgtcatga caagcacaag atccagatct gcccaataag
     3481 gcgtcagcga ctcagccgag gttccgggct tgagcgaaat tccggcccgg gcacccaatg
     3541 atttgatttc ggcaagacac tttcctacgt ccttttccgc ctcaacgtga atggtaatcc
     3601 agtcggcgcc ggccttgcga aaatcagcaa tataccggat cggttgatcg atcatcaaat
     3661 gcacgtccag aggcagggaa gttactttcc ggatcgcctt gaccacaaga ggtccgatcg
     3721 taaggtttgg cacaaaatgt ccgtccatca catccacgtg tagaacatca caaccggcac
     3781 gttcaacatc ccgaatctct tccgccaagc ggctgaaatc cgcggacaga agactggggg
     3841 cgatcaaaac acgtctcatt ctatcaccaa tcccttccgg attgcctttt gcatgggctc
     3901 attaaccggc cccaccacgg ccagattgag tcgttgcgtg cggaaaagcc tcctcgcgac
     3961 ttcccgaatt tcttcctggg tcacttcttc aatttttcgc cggatatctt ctttattggg
     4021 caattcattc gcgcaaagca gtttttcccc cgcccataac agatgatcca gcgtgtcctc
     4081 aacgccgaga taaacctgac tcataaaata gtccttggcg cgccgcaatt ccccgggcgt
     4141 caccattttt cgtgtcactt tgtccagttc tttcagaatt actcgcacgg caagttccgt
     4201 tttgacggtc tccacgccgg cagaaatggt aaaggctccg gcatccgcgt aagcagaaac
     4261 gccggatttg atctcatagg ccaacccgcg tttttcccgg acttcatcaa acaagcggct
     4321 tgacatattc gcgcccagaa taatgttaag aagtcccagc ttgtagcgat ccggatgcat
     4381 cctcgcgagg gcatgaaacc ctatcacaaa atgggtttgt tccgtttttt tctcatgaaa
     4441 acagatccgg ggt
//
LOCUS       DAOSIO010000138         4400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_17554_length_4400_cov_3.321059, whole genome shotgun sequence.
ACCESSION   DAOSIO010000138 DAOSIO010000000
VERSION     DAOSIO010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17554_length_4400_cov_3.321059"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PK590_05755"
     CDS             <1..287
                     /locus_tag="PK590_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24134.1"
                     /translation="SNLSKQQIFFVVFQSDQPHVKDQNQTWLDGLQEKGYPLKLVEVP
                     DGKGFESLELKKEAYDWIEATGQSFSAAVEKSQRGFKAKFKKGVKDFFTV"
     gene            306..1991
                     /gene="ilvB"
                     /locus_tag="PK590_05760"
     CDS             306..1991
                     /gene="ilvB"
                     /locus_tag="PK590_05760"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOG24135.1"
                     /translation="MELTGAQILVKSLEMEGVKHIFGLPGGVILPTYDVLYDSKIKLI
                     LTKHEQGATHMADGYARASGKPGVCMVTSGPAATNTVTGLATAYMDCVPMVCITGQVV
                     TNVIGSDAFQEVDIVGITRPITKHNFLIKNVKEIAKTVREAFYIATTGRQGPVLIDFP
                     VDVSRAKTEFVWPERVFIRSYRPDLPNPDASSQICEAARLINQSKRPVLYVGGGAISS
                     GAEKEIRALVEKTGIPITTTILGLGIFPETHPLSLRMLGMHGTHYANYSVQNSDALIG
                     IGARFDDRVTGNVAKFAPRAKIIHIDIDPSSISKTVRAHVPVVSDVKVAVKELLGLVG
                     DRRAEIKPWLDQIALWKEKHPMKYDRKSAKVTQEHIIEQIGELTRHEAVVVTGVGQHQ
                     MWTAQWYKFKKPRTMLSSGGLGTMGYGFPASIGAQIACPKATVVCIEGDGSFQMTMTE
                     LATAAFNKTPIKVFIMDNGYYGMVRQWQELFYKRRYSGSVIGPTNPDFVKLVESYGIR
                     ALEIRTKKDVCPVIKKALAHRGPVVVHCLVAPESNVYPMVAAGNALDQIMDMA"
     gene            2072..2617
                     /gene="ilvN"
                     /locus_tag="PK590_05765"
     CDS             2072..2617
                     /gene="ilvN"
                     /locus_tag="PK590_05765"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOG24136.1"
                     /translation="MKHTISVLVENHFGVLARVAGLFSARGFNIDSLAVGETHDPQVS
                     RMTVIAQGDDHVVDQIMKQLGKLIDVICVEDLTNKDVIERELVLVKVGANAENRNDIM
                     HVVNTFRAKIVDVSPGTMTIEVTGSEGKIDAMLELLKPFDLREVVRTGQIAMSRRHEL
                     KMPGAKGARKASHKKTAKKKK"
     gene            2648..3646
                     /gene="ilvC"
                     /locus_tag="PK590_05770"
     CDS             2648..3646
                     /gene="ilvC"
                     /locus_tag="PK590_05770"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOG24137.1"
                     /translation="MSAKIYYDKDADLNLLKGKKVAIIGYGIQGRGQGLNLRDSGVDV
                     IVSELPGTPNYEMAIKDGFKPVAATDAAQEADIIQILTQDHLQATIYEKQIAPHLKKG
                     KSLCFSHGFNIHFGYIKPAPEIDVFMVAPKGPGALVRSQFVEGGGVPCLVAIKQNASG
                     KALEIGLAYAKAIGGTRAGVIETTFKEETETDLFGEQAVLCGGLSELITAGFDTLVNA
                     GYQPEMAYFECLHEVKLIVDAMFVSGISGMYKRVSDTAEYGGYSRGSRVVTAETREEM
                     KKMLEEIVSGKFAKEWMDENKQGCPNFKKMRETCQQHPIESTGEKLRKMMEFINKK"
     gene            3719..>4400
                     /locus_tag="PK590_05775"
     CDS             3719..>4400
                     /locus_tag="PK590_05775"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149330.1"
                     /note="catalyzes the formation of 2-isopropylmalate from
                     acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOG24138.1"
                     /translation="MKSARKIYIFDTTLRDGEQSPGASLTPEEKLKIAKQLERLNVDV
                     IEAGFPASSEGEMKAVSLVAEQVKKPVICGLSRMVPKDIDACRRALEKAAKKRLHVFL
                     ATSQIHRDFKLKKNKREIIEMAVKNIRSAKKDFRQIEFSPEDASRTERDFLAQVVRAA
                     IDAGATSINIPDTVGYTVPQEFSELMRFLIKKNPELGKTVVLSVHCHNDLGLATANSL
                     AAILAGANQ"
BASE COUNT         1149 a          954 c         1265 g         1032 t
ORIGIN      
        1 gttccaattt gtctaaacaa cagatttttt tcgtggtgtt tcagagcgat caaccccatg
       61 tcaaagatca aaatcagaca tggcttgatg gtttgcagga aaaagggtat cccctgaaac
      121 tggtggaagt cccggacgga aagggttttg aatcgttgga actgaagaaa gaagcctacg
      181 actggattga agctaccgga cagagttttt cggcggcggt tgaaaaaagt caaaggggat
      241 ttaaggcgaa gttcaagaaa ggcgtcaaag atttttttac ggtctaattt aaaaggaaat
      301 ttattatgga actaaccggt gcgcagattt tggtcaaaag tcttgaaatg gagggagtga
      361 agcacatctt cgggctcccc ggaggggtga ttcttccgac gtacgacgtt ctttacgatt
      421 caaagattaa gctgattctt acgaaacacg agcagggtgc gacacacatg gcggacgggt
      481 acgcgcgcgc gagcggtaag cccggcgtct gtatggtaac ttcgggcccc gcggccacga
      541 atacggtgac cggcttggcc acggcctata tggattgtgt gcccatggtt tgtattacgg
      601 ggcaggtggt gacgaatgtg atcgggagtg acgcgtttca ggaagtggat attgtcggaa
      661 ttacgcgccc cataacgaaa cataattttt tgattaagaa cgtgaaagag atcgcaaaaa
      721 ctgtgcggga ggcgttttac atcgcgacga caggccgaca gggccctgtg ttgattgatt
      781 ttcccgtgga tgtcagtcgc gcgaaaaccg aatttgtgtg gccggaaaga gtttttattc
      841 gttcgtatcg gccggatctt cccaaccccg acgcgtcgag tcagatttgc gaggcggcac
      901 gcctgatcaa tcaaagtaag cggccggttc tctatgtcgg tggtggcgcc attagttcag
      961 gcgcggaaaa ggaaatccgg gcgctggtgg aaaaaacggg aattcccatt acgaccacca
     1021 tcctcggtct cgggattttc ccggagacgc acccattgtc cttgcgaatg ctggggatgc
     1081 acggaactca ttacgcgaac tattccgtcc agaattcgga tgccctgatc ggcattggcg
     1141 cgcgctttga tgatcgtgtg accgggaatg tcgccaaatt tgcgccgcgg gcgaagatca
     1201 ttcatatcga cattgatcct tcctcgattt cgaaaacggt cagggcccat gtgccggtgg
     1261 tgagcgacgt gaaggttgcc gtcaaagaat tactcgggct tgtcggggat agacgcgcgg
     1321 aaatcaagcc ctggctggac cagatcgcgt tatggaaaga aaagcatccg atgaaatatg
     1381 atcgtaaatc cgctaaagtt acgcaggaac acatcataga acagatcggc gaattgactc
     1441 ggcatgaggc ggtggtagtg accggcgtgg gtcaacacca gatgtggacg gcccagtggt
     1501 acaagtttaa aaaacccagg accatgttgt cttcgggagg acttgggacc atgggatatg
     1561 gttttccggc gtcgatcggg gcgcagatcg cttgtcccaa ggcgacagtg gtttgcatcg
     1621 aaggcgacgg ttctttccag atgaccatga ctgaactggc aacagcggct tttaacaaga
     1681 cgccgatcaa ggtttttatc atggacaatg gttactatgg aatggttcga cagtggcagg
     1741 agctttttta caaaagacgc tattcgggtt cggtaattgg tcccacaaat cccgattttg
     1801 tgaaactggt ggaatcttac gggatccgtg ctcttgaaat aaggacgaag aaggatgtgt
     1861 gtcctgtgat caaaaaagcc cttgcgcacc gaggtcccgt tgttgtccat tgtctggtgg
     1921 cgcccgaatc aaatgtgtat ccgatggtcg cggccggtaa cgcgcttgat caaattatgg
     1981 atatggcctg acacgggccg gcgggtttca gggatagcgg gtaaaaaagt tttttaccgc
     2041 tttttcctaa ttcctaaaaa aggagtttga tatgaaacac acaatttcag tgctggtgga
     2101 gaatcacttt ggtgttttgg cgcgtgtggc ggggcttttt tctgcgaggg gctttaatat
     2161 cgacagcctt gccgtgggag aaacccatga ccctcaggtg tcacgcatga ctgtgatcgc
     2221 gcagggggat gatcatgtgg tggatcagat aatgaaacag ctcgggaaac tgatcgatgt
     2281 catttgcgtt gaagatctga cgaacaaaga cgtgatcgaa agggagcttg tgttggtcaa
     2341 ggttggtgcc aacgctgaaa accgaaatga tatcatgcat gttgtgaata cttttcgggc
     2401 gaaaatcgtg gacgtgagtc ccgggaccat gacgatcgag gtaacgggaa gcgaagggaa
     2461 aattgacgcc atgcttgaac tcttgaaacc gttcgatttg cgcgaagtcg tgaggaccgg
     2521 acagatcgcg atgtcccggc ggcatgagct taagatgccc ggtgccaaag gcgcccgtaa
     2581 agcttcacat aaaaaaactg ccaagaaaaa gaaataattt caattaacaa ccagaaagga
     2641 aactgaaatg tcagcaaaaa tttattacga caaggacgca gacctcaatc tgctcaaggg
     2701 gaagaaggtt gcgattatcg gttatggaat tcaggggcgt ggacagggtt tgaatcttcg
     2761 cgattccgga gtggacgtga ttgtttcaga gctccccggg acaccgaatt atgaaatggc
     2821 cattaaagat ggtttcaagc cggtcgcggc aaccgacgcg gcccaagagg cggacattat
     2881 ccagattttg acccaggatc atcttcaggc cacaatttat gaaaaacaga tcgctcccca
     2941 tctaaaaaaa ggcaaatcgc tttgtttttc ccacggattt aatatccact ttggttacat
     3001 caaaccggcg ccggaaattg acgtatttat ggtcgcgccg aaaggcccgg gcgcgctagt
     3061 tcgttcccag tttgtggaag gcggcggggt tccatgtctc gtggcgatca agcaaaacgc
     3121 gagtggcaag gctctcgaga tcggactggc ttatgcgaag gcgatcggcg ggactcgcgc
     3181 gggcgtgatt gaaaccacgt tcaaggaaga gactgaaacc gatctctttg gcgagcaggc
     3241 ggttctttgt ggaggtctta gcgaattgat taccgccggg tttgatacgc tggtcaacgc
     3301 gggctatcaa cctgaaatgg cctattttga atgtctccac gaagtgaagc tgattgtgga
     3361 cgcgatgttt gtttcgggca tttcagggat gtataaaagg gtgtccgata cggcggaata
     3421 tggcggatac agccgcggct cccgtgtggt gacagcggaa acccgcgagg aaatgaaaaa
     3481 aatgctggaa gaaatcgtaa gcggaaagtt cgcgaaggaa tggatggatg aaaacaaaca
     3541 agggtgcccg aatttcaaaa aaatgcggga aacttgtcag caacatccca ttgaatcaac
     3601 cggcgaaaaa cttcgcaaaa tgatggagtt tattaacaag aagtaaaaag catggttggt
     3661 tgcctcgtgg tacggggttt tcgcaatcca ttaaggaaag agaaacggta ttgaacccat
     3721 gaagtcggct cggaagatat atatttttga cacgacgttg cgagatggcg agcaatcgcc
     3781 gggggcttct ctgactcccg aagaaaaact caaaatcgcg aaacagcttg agcgtctcaa
     3841 cgtcgacgtc attgaagcgg ggtttcccgc ttcctcggaa ggtgagatga aggctgtgag
     3901 cctggtggcg gaacaggtaa aaaaacccgt gatttgcggc ctttctcgca tggttcccaa
     3961 ggatattgac gcttgccgcc gggctctcga aaaggccgca aaaaaacggt tgcacgtgtt
     4021 tctcgcgacg tcgcaaattc atcgtgattt caaacttaag aaaaacaagc gtgaaatcat
     4081 tgagatggcg gtcaagaata ttcgttccgc caagaaggat ttccgccaga ttgagttctc
     4141 gccggaagac gcctcgcgga ctgaacggga ttttcttgcc caggtggttc gggccgcgat
     4201 tgacgccggc gcgacctcaa ttaacattcc tgacacagtt ggttatacgg tgccccagga
     4261 gttttccgag ctcatgaggt ttttaatcaa gaaaaatccg gagcttggca aaacggtagt
     4321 tttgtcggtt cactgtcata acgatcttgg tctcgcgacc gcgaattcgc tcgcggcgat
     4381 tttggccggt gctaaccagg
//
LOCUS       DAOSIO010000139         4394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_17583_length_4394_cov_4.945379, whole genome shotgun sequence.
ACCESSION   DAOSIO010000139 DAOSIO010000000
VERSION     DAOSIO010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17583_length_4394_cov_4.945379"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="PK590_05780"
     CDS             <1..910
                     /locus_tag="PK590_05780"
                     /inference="COORDINATES: protein motif:HMM:NF020073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG24139.1"
                     /translation="SNDGLLVSCYEKCGLKGVGVEPAKNLSADANQRGIVTLNRYFDL
                     ETVREITARFGKAQVVSANNVFAHIDDIQSVIRNVRELIADDGFFVIEFAYLVTMLEK
                     MYFDMVYHEHVCYIGETALHRFAERFGMELFDIKAVDTHGGSLRVFMQKKGGPRTREK
                     VVGDYLAAESEKGCLGDGIYHDFSKRVLAFRSKLNDMLSDIKRSGKTVCGYGAPAKAT
                     TIINFCGLTPATIDYIVDDNPLKQGRLVPGSGIPIVPSSHLHASPAHYVLIFAWNFAP
                     EIIKKIEFLKEKGVRFIIPLPEPLIV"
     gene            992..2488
                     /locus_tag="PK590_05785"
     CDS             992..2488
                     /locus_tag="PK590_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG24140.1"
                     /translation="MASQSNHSGEARPGQERALKVADLFENSYDLIQIFTPEGQLLEV
                     NPSWRETLECPEDTLPDSVYSVIHPRQHDEFRKAVAEALNGESLRTVSITLITRSVRE
                     ILVEGNLASVPSGDGKVLIRGIFHEITKQKQYEELRDELIGTVSHELRTPLTVIREGA
                     SQIYDGLAGDIVPAQKEILTMILHGIDRLSRIINDLLDASRLESGKVQLRRAPCNMPE
                     IVREVIRNFEGITAAKGIDLQMETSGERVEVYLDRDKFIQILTNLISNAIKFTTKGWV
                     KVSIRETEGFVECKVSDTGRGISGEDLPRVFEKFRQFQRLEGSGIKGTGLGLLICKKL
                     VELHHGSITIDSYPLQGTTVTLLFPRYTSREVFREQINLALGRCVEEGGTLSLIVFDL
                     VDFEALKSKLGELPFQDAILKMEAMINENLRRAADLAIKDAKAILVLLPDTKRENAYI
                     VLGRLYQILEDYLSKTWGKSRIKIHSSIAVFPEDAKTVDEILEKIYEA"
     gene            2605..3486
                     /gene="metF"
                     /locus_tag="PK590_05790"
     CDS             2605..3486
                     /gene="metF"
                     /locus_tag="PK590_05790"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOG24141.1"
                     /translation="MRISSFYQTGRPAISFEFFPPKTPEGEQKLYASVAELKELKPSF
                     VSVTYGAMGNTQANTLRVVTEIQNRIGIETAAHLTCVAHSRDEIAGILGKLAEAHIEN
                     IVALRGDPPQGETEFRPAPNGFRYASELVSFIRSHRVYGRAFDLVVAGYPEGHPECRD
                     LEKDLEHLKIKVDSGADAINTQLFFDNRDYFRFVERARKAGIRIPIVPGIMPVTYGPQ
                     IEKFSKMCGAKIPLEIREAIEKFGDDQSSILRFGIDYAIRQCRELLAEGAPGLHFYTL
                     NQSCATREIYAHLGLPR"
     gene            3513..3965
                     /locus_tag="PK590_05795"
     CDS             3513..3965
                     /locus_tag="PK590_05795"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24142.1"
                     /translation="MANWHIGEILIQKRLIDWEQLNEALSEQKLTRESVGEILVRKQR
                     IPRFLLYKALAERHAMPFADLSRTHIDEAAIRRVPQSIAQKYGIIPIEIRGRTLVIGI
                     GDPTAVIPRDEIASLAKMNSIHTVLCTPEAVRIATAAHYGKTTLEAES"
     gene            3962..>4394
                     /locus_tag="PK590_05800"
     CDS             3962..>4394
                     /locus_tag="PK590_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019638214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOG24143.1"
                     /translation="MKKVKMRLEKDSLGSKAVPSAAYYGVETVRAVENFPISGERFHG
                     DFIWALATVKKACAQANMTLRRLDPRKGRAIVRACDEVISGKFRQEFVTDVLQSGAGV
                     SAHMNMNEVIANRALEILGKKKGDHGFLHSHDHVNMGQSTND"
BASE COUNT         1081 a         1076 c         1214 g         1023 t
ORIGIN      
        1 cagcaatgac ggccttctgg tttcttgcta cgaaaaatgt ggcctgaaag gggtcggcgt
       61 ggaacccgcg aaaaacctct cggcggatgc caaccagcgg ggtattgtga cgctgaaccg
      121 ctattttgat ctggagacgg tcagggaaat cacggcccgc tttggtaaag cccaggttgt
      181 gagcgcgaat aatgttttcg cccatatcga tgatatccag tccgtgatcc gcaatgtccg
      241 cgaattgatc gcggatgatg gcttttttgt catcgaattc gcctaccttg ttaccatgct
      301 cgaaaaaatg tattttgaca tggtctacca tgaacatgtt tgttatatcg gcgagacggc
      361 gcttcatcgt ttcgcggaga ggttcgggat ggagcttttc gacattaaag ctgtcgacac
      421 gcacggggga tcacttcgtg tttttatgca aaagaaaggc gggccgagga cgcgggaaaa
      481 ggttgtgggg gattaccttg cggctgaatc ggagaagggt tgtctcgggg acgggatcta
      541 ccacgatttt tcgaagcggg tccttgcgtt tcgatcaaaa ctcaatgaca tgctgtcgga
      601 catcaaacga agcggaaaaa ccgtttgcgg ttatggcgcc cccgcgaagg cgacgacgat
      661 catcaatttt tgcggcctta ctccggcgac gattgactat atcgtggacg acaatcccct
      721 gaaacagggg aggttggtgc cgggttccgg aattccgatt gttccgagtt cacacctcca
      781 tgcgtcgccg gctcattacg ttctcatctt tgcctggaac tttgcgcccg aaatcataaa
      841 aaaaatagaa tttttgaaag aaaaaggcgt tcggtttatc attcctctgc ccgagcctct
      901 gattgtgtga gtcctttttt aaaaagaaaa aacacaacgc tgtttggagt tcaatgcccc
      961 caaaaaatct ttgagggaat aaggagtatc catggccagc caatctaatc actccggtga
     1021 agcccgaccc ggccaggaac gcgccctcaa ggttgccgac ctttttgaaa actcttacga
     1081 cctgatacag atatttaccc ccgaaggaca gcttctcgag gtgaatcctt cctggcgcga
     1141 gactctcgaa tgtccggaag acactttgcc ggactcggtt tatagcgtga ttcatccccg
     1201 acagcacgat gagttccgga aagcggttgc tgaggccctg aacggggaat ctctccggac
     1261 agtctccata actctgatca cgcgatctgt ccgggagatc cttgttgaag ggaacctggc
     1321 ctctgttccc tccggagacg ggaaggtttt gatacgcgga atttttcacg agatcacaaa
     1381 gcaaaaacag tatgaagaac tcagggatga attgatcgga acggtttcgc atgaactgag
     1441 aactccgctg accgtgatcc gtgaaggggc ctcccagatc tatgacggtc tggcaggtga
     1501 cattgtaccc gcccaaaaag agattctgac catgattctc cacgggattg accgcttgtc
     1561 gaggatcatc aatgatctcc tggatgcctc cagacttgaa tccggcaagg ttcagctccg
     1621 ccgggcccct tgcaatatgc cggaaatagt tcgtgaggtg atccggaatt tcgaaggaat
     1681 taccgccgcc aaagggattg atctccagat ggagacttcc ggagagcggg tggaagtcta
     1741 tctggaccgt gataaattta tccagatcct gaccaatctc ataagtaacg cgatcaaatt
     1801 tacgactaag ggatgggtca aggtttccat tcgcgagacc gaaggatttg tggaatgcaa
     1861 ggtgtcggat accggaaggg gaatttcagg cgaagatctc cccagagtat ttgagaaatt
     1921 ccgccagttt caacgcctgg aaggttccgg aatcaagggg acgggactgg gattactcat
     1981 ttgtaaaaaa ctggtcgaac ttcaccacgg aagtattacg atcgacagtt accctcttca
     2041 ggggactacc gtgaccctac ttttccccag gtacacttct cgggaagttt tccgggagca
     2101 aatcaacctg gctctcgggc gatgtgtcga agaaggcggg acgctttccc tgattgtttt
     2161 tgacctggtg gattttgaag cgctgaaatc aaaactgggg gagttgccgt ttcaggacgc
     2221 tattttgaaa atggaagcca tgatcaatga aaatttgcgg cgtgcggcag acctcgcgat
     2281 taaggacgcg aaggcgatcc tggtgctgtt acccgatacc aaaagggaaa atgcctatat
     2341 tgtcctgggg cgtctctatc agattcttga agattatctt tcaaagactt gggggaaatc
     2401 gcgtatcaaa atacactcga gcatcgcggt attccccgaa gacgcgaaaa cagttgatga
     2461 gattcttgaa aaaatttacg aggcgtgatg cgccggatcc ggaatgattt ttccagagtt
     2521 cgcgccggac ttgaatcgag cgccgaaaaa atttttacaa atttcctaag aatatccgga
     2581 ttcctgcggt aaaatcctct tcctatgaga atttcttctt tttatcagac aggtcggccg
     2641 gcgatttcct tcgagttttt tccgcccaaa actcccgaag gggaacagaa gctttatgct
     2701 tcagtcgccg agctcaagga actgaagcct tcttttgtgt ccgttactta cggcgcgatg
     2761 ggaaacaccc aggccaacac gctccgagta gtgacggaaa ttcagaaccg gatcggtatc
     2821 gagacggcgg cccatttgac ctgcgtggcg cattcccgcg atgagatcgc ggggatcctc
     2881 ggcaagcttg cggaggccca tatcgaaaac attgtcgcgc ttcgggggga tccgccgcag
     2941 ggggaaacgg aattccggcc cgctccaaac ggattccgtt atgcctcgga gctggtcagc
     3001 tttatccgct cccaccgtgt ttacggccgg gcctttgatt tggtggtggc tggttatccc
     3061 gaaggacatc ccgagtgccg cgacctggaa aaagatctcg aacatctcaa gatcaaggtg
     3121 gactccgggg cggacgcgat caacacacag ctttttttcg ataaccggga ttatttccgg
     3181 tttgttgaac gggcgcgaaa ggcggggatc agaatcccga tagttcccgg gatcatgccc
     3241 gtgacctacg ggccccagat tgaaaaattt tcaaagatgt gcggggcgaa aattccactt
     3301 gaaatcaggg aggctatcga aaagtttgga gacgatcagt cctcgattct ccggttcggt
     3361 attgattacg cgatccggca gtgccgggag cttctcgcgg aaggcgctcc gggacttcat
     3421 ttttacactt taaatcaatc gtgtgccacc cgggaaattt atgctcatct cggtcttcca
     3481 cggtaaacaa ggtgcggtaa ccaaaagaac ttatggcgaa ctggcatatc ggtgaaatcc
     3541 tgatacaaaa aaggctgatc gattgggagc aactgaatga ggcgttgagt gagcagaagc
     3601 ttacgcgtga gtccgtgggg gagattttgg tgcgcaaaca acgaatcccg cgctttcttc
     3661 tctacaaggc gctcgcggaa cggcacgcga tgcctttcgc ggatctttcc cgtacccata
     3721 ttgatgaggc cgcgatccgc cgggtgccac aaagcatcgc tcaaaaatac ggcatcattc
     3781 cgatcgaaat ccgggggaga acgcttgtga tcgggatcgg ggatccgacg gcggtcattc
     3841 cgcgggacga gattgcctcg ctcgcgaaaa tgaactcgat tcatacggtg ctttgcacgc
     3901 cggaagcggt caggatcgcg acggcggccc attacggaaa aacgacgttg gaggcagaat
     3961 catgaaaaag gtgaaaatgc gtctggaaaa agactcgttg gggagcaagg ccgtcccctc
     4021 agcggcttat tacggtgtcg aaaccgttcg cgcggttgag aactttccga tttcggggga
     4081 gcggttccac ggcgatttta tctgggccct cgcgacggtc aaaaaggcgt gcgcccaggc
     4141 gaacatgacg ctcaggcggc ttgacccgag gaagggaagg gccatcgtcc gggcctgcga
     4201 cgaggtcatc tcgggaaaat tcagacagga atttgtcacc gatgtccttc agtccggggc
     4261 gggggtttcc gcgcacatga acatgaacga agtcattgcc aatcgcgcgc tcgaaattct
     4321 cggaaagaaa aaaggcgatc acgggtttct tcattctcat gaccacgtga acatgggaca
     4381 atccacgaac gacg
//
LOCUS       DAOSIO010000140         4376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_17683_length_4376_cov_4.839620, whole genome shotgun sequence.
ACCESSION   DAOSIO010000140 DAOSIO010000000
VERSION     DAOSIO010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17683_length_4376_cov_4.839620"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /locus_tag="PK590_05805"
     CDS             <1..1428
                     /locus_tag="PK590_05805"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOG24144.1"
                     /translation="KNSVEEQLAQKGIEPFQVESVQSDEMGVKLVYSAISLSYKEKPD
                     EILPSVHPGNLDELEYALVSKIYRMSLDEIPGIAMVAPAETRGVDPNLRALLEQLGAG
                     GQLQQSFRDDPYEALENGLRYDGYRVERIDLTEASPIPPGTKALALIAPERLSERQKY
                     EINKFIVQGGSVFVAVQHREFNYRPMANNVELIATDKDPQINDLLGAWGLTVDSRVLA
                     DEQSEIVNVSAGRVGLFETQFPVRLPIQIVLTPAEMNLKISITSRLPAFFYLWGSAIT
                     LDQKKIEAQGLSVETLLSSSKDSWMVPFKPQGMTARDLSQEAADIQGPFSLAVLVQGQ
                     FADAFEKIEKVPAWESENTPGDEETVPEKKTEDEAHQNLVPAPGKMILIGAATPFQRQ
                     LIRGGGHMAFFMNAMDVLTLGDDLVGIRTKGVATHALPKVSREAKLFWRFFGTLFMPA
                     LIAVAGFLHFYFRNRARQAYLKNIA"
     gene            1438..2475
                     /locus_tag="PK590_05810"
     CDS             1438..2475
                     /locus_tag="PK590_05810"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOG24145.1"
                     /translation="MRRKQILFLFLILAFLSAGLWARFFLNHRQGSDVAGVDEKLLTF
                     DPGAAERILIGKGLKPQVEIFKEDGIWKVRELWNARADEVKVQAILSALSGIRGDLRS
                     SDPALLKDFGITDPEAFCVKVMGGNNDLLADLKIGVKSPGHTGLFVRLFDRPDVYAVE
                     VGLDRMLGLFTGLEEGDLSSGFWADLVLFKLEPERVKEITISRLKDGVTALRAGVVKE
                     KGGGPGEPGRWKLIGPQESLSADPDKVLRFIVTLNSLRAQAVADPNQKHGLDHPVWQL
                     EVKESEGGKEKETVLFAGAKDSKEYVYFVKRRDRSEVYRLNGYYFEDMNITNEKLIED
                     TPRGDEGTGNS"
     gene            2472..3266
                     /locus_tag="PK590_05815"
     CDS             2472..3266
                     /locus_tag="PK590_05815"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOG24146.1"
                     /translation="MRLYLDLLRHILDHGHEKKDRTGTGTISTFGYQMRFDLSRGFPL
                     LTTKKVHLKSVIYELLWMLRGDTNIRFLKDHGVTIWDEWADPAGDLGPVYGAQWRSWR
                     TKEGGIVDQIAQAVEMIRKNPESRRIIVSAWNVGELHQMALMPCHAFFQFYVAGGKIS
                     CQLYQRSADVFLGVPFNIASYSLLTLMMAQVTGLEAGEFIHTFGDVHIYLNHREQVRL
                     QLSRNPRPLPVMRLNPAVKDIFGFQYEDFNLENYDPHPAIKAPVAV"
     gene            3331..3828
                     /locus_tag="PK590_05820"
     CDS             3331..3828
                     /locus_tag="PK590_05820"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOG24147.1"
                     /translation="MLFHIVAVANQRVIGKANQLPWHFPKDLQHFKGTTFGQTVIMGL
                     RTFESLGCKPLPGRENIVLDRSGQNPYPGQTYYTTLDEALKNIKTRDAFIIGGAELYR
                     TTIDLVDGIYLTRIYADYEGDVLYPEIPSEFTEKKRYKLQDNPTLEVIYYEKNLKKNC
                     CCCGE"
     gene            3818..>4376
                     /locus_tag="PK590_05825"
     CDS             3818..>4376
                     /locus_tag="PK590_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG24148.1"
                     /translation="MANKNIYFKQIELGPMANFLYLIGDKNAGVCAVVDPAWDIRAVL
                     DAAGVDGMRVTAGLLTHTHFDHAHGVEDLIKLTQCKIYVHEREAKYLKEFQPHLIETR
                     EGMTIKIGDVEILVLHTPGHTEGGQCFLVDGILISGDTLFPGACGRCDLPGGNEEVLR
                     KSLKRLAGLDERIKVYSGHAYGAESS"
BASE COUNT         1061 a          996 c         1249 g         1070 t
ORIGIN      
        1 aaaaattccg tcgaggaaca gttggcccaa aaaggaatag agccgtttca ggtggagtcg
       61 gtgcaatccg atgagatggg ggttaagctc gtttattcgg cgattagcct ttcttacaaa
      121 gaaaagccgg atgaaattct tcccagtgtc catcccggca acctggacga gcttgaatac
      181 gcgcttgtgt ccaagattta ccgcatgtct ttggatgaaa tcccggggat cgcgatggta
      241 gctcccgccg aaacaagagg cgtggacccc aacctgaggg cgcttcttga gcagttgggc
      301 gccggaggtc agttgcagca aagtttcagg gatgacccgt atgaggcgct tgagaacgga
      361 ttgcgatatg acggataccg cgtggaaagg attgatctta cggaggcttc tcccattcct
      421 cccggcacga aagctctcgc gctgatcgcg cctgaacggc ttagtgaacg ccagaaatat
      481 gaaatcaata aatttattgt ccagggaggt tccgtattcg tggccgtaca gcaccgcgaa
      541 ttcaactatc ggcccatggc caataacgtc gaactgatcg cgaccgacaa ggacccgcaa
      601 atcaatgatc ttctgggggc ctggggattg acggtggatt cgcgggtgct tgcggatgag
      661 caaagcgaaa tcgtcaacgt ttctgccggg agagtgggcc tttttgaaac tcagtttcct
      721 gtgcggttgc ctatccagat agttttgact cccgcggaaa tgaatctgaa gatttcgatc
      781 acgtcccggc ttccggcgtt tttttatctt tggggaagcg ccattaccct cgatcaaaaa
      841 aagatcgaag cccaggggct cagtgtggaa acgctccttt cttcttccaa ggattcctgg
      901 atggtcccat ttaagcccca gggcatgacg gcccgtgatc tgtcacagga agcggcggat
      961 attcaggggc cgttttcttt ggcggtcttg gtgcaggggc agtttgccga tgcttttgaa
     1021 aaaatcgaaa aagttcccgc ctgggaaagc gaaaacacgc ccggtgatga ggagacggtt
     1081 cctgaaaaga agaccgaaga tgaagcgcac caaaaccttg tgccggcgcc cgggaaaatg
     1141 attttaatcg gagcggccac gccgtttcaa aggcaactga ttcgcggcgg ggggcacatg
     1201 gcgtttttca tgaatgcgat ggatgttctg accctcggag atgatctggt cggaattcgg
     1261 acaaagggtg tggcgaccca cgctcttccg aaagtgtccc gtgaggcaaa gcttttctgg
     1321 cgttttttcg gaaccctttt catgcctgcc ttgattgcgg tcgcgggatt tttacatttt
     1381 tactttagaa acagggcaag acaggcctat ctcaaaaaca ttgcctgagc gaaccttatg
     1441 agacgaaagc aaattttatt tctctttctg atactggcgt ttttgtcggc gggactttgg
     1501 gcccgttttt ttctaaatca tcggcaagga agtgatgtgg cgggagttga cgaaaagctt
     1561 ttgacgtttg atcccggcgc ggcggaacgt attctgatcg gcaaggggtt gaagccacag
     1621 gtcgaaatat tcaaagagga cggaatctgg aaggtcaggg agctttggaa tgcccgggcg
     1681 gatgaagtca aggttcaggc gattctttcg gccctttccg gaattcgggg agatctgaga
     1741 agttcggatc ccgcgctttt aaaagatttt gggattacgg acccggaagc tttttgtgtg
     1801 aaggtcatgg gtggaaacaa cgatctgttg gcggatttga aaattggcgt taagagcccc
     1861 ggccacacgg gcctttttgt ccggcttttt gatcgtccgg atgtttacgc cgtggaagtc
     1921 ggattggatc gtatgctggg tctttttacc ggtcttgagg agggagatct ttccagcgga
     1981 ttttgggcgg atcttgtctt gtttaagctg gagcctgaac gcgtgaagga aatcacgatt
     2041 agtcggttga aagacggggt gaccgctctt cgggcaggtg ttgtgaaaga aaagggagga
     2101 gggcctggag aacccggacg atggaaactg atcgggcccc aggaatcgtt atcggcggat
     2161 cccgataaag ttctccgttt tattgtgacc ttgaacagcc ttcgggccca ggcggttgca
     2221 gatccaaatc aaaaacacgg cctggaccat ccggtttggc aactggaggt gaaggaatcg
     2281 gaaggcggga aggaaaaaga aacggtcctt ttcgccggcg cgaaagattc aaaggagtat
     2341 gtttatttcg tcaaacgccg ggatcggtcc gaagtttacc ggttaaacgg ctattacttt
     2401 gaagacatga atattaccaa cgaaaaactg atcgaagata ccccgcgagg ggacgaagga
     2461 accgggaact catgagactt tatctggatc ttctccgtca tattcttgat catgggcatg
     2521 aaaaaaaaga ccggacgggc acgggaacga tcagcacttt cgggtatcaa atgcgctttg
     2581 acctgtcgcg gggttttccg cttctgacga caaaaaaagt tcatcttaaa tccgtgatct
     2641 atgaactgct ctggatgttg aggggggaca ccaacatcag gtttttgaag gatcacggcg
     2701 tgacgatttg ggacgaatgg gcggatcctg ccggtgatct gggccccgta tatggcgcgc
     2761 agtggcgttc ctggcggaca aaggaaggcg gaattgtgga tcagatcgcc caggcggtcg
     2821 agatgatccg aaaaaaccct gaaagccggc gcatcatcgt gagcgcctgg aacgtggggg
     2881 aacttcatca aatggccctg atgccctgtc acgcgttttt ccagttttac gtggccggag
     2941 ggaaaatttc ttgtcagctt tatcagcgca gtgccgatgt ttttcttggc gttccattta
     3001 atatcgcgtc ctattcgctt ctgactctga tgatggcgca agtcacgggg cttgaggcgg
     3061 gcgagtttat ccacacgttt ggtgatgtcc atatttacct taaccatcgg gagcaagtcc
     3121 ggttacagct ttcaaggaac ccccggcctt tgcccgtgat gcgattgaat cccgcggtaa
     3181 aagacatttt tggatttcaa tacgaagatt ttaatctgga aaattacgat cctcatccag
     3241 ccatcaaggc gcccgttgcc gtttaaaacc ggacccatct gttccgcgcg attgagaccg
     3301 cgggcagggt ccaagaggga ggttttatcg atgttatttc acattgtcgc cgttgccaac
     3361 cagcgagtga tcggaaaggc caatcagctt ccctggcatt tcccgaaaga tttacagcat
     3421 ttcaagggaa ctacgttcgg tcagacggtg atcatggggc ttagaacatt tgaaagtctt
     3481 ggctgtaagc cattgccggg gcgggagaac atcgtgctgg accggagcgg ccagaatcct
     3541 tatcccggac agacttatta cacgaccctt gacgaggctc ttaaaaatat caagacacgg
     3601 gacgccttta tcatcggcgg ggcggaactt taccgaacca caattgatct tgttgatgga
     3661 atttatttga cccgaattta cgccgattat gagggggacg tgctttatcc cgagatcccg
     3721 tcggaattta cggaaaaaaa gcggtacaag ctccaggata atccgaccct tgaggttatc
     3781 tattacgaaa aaaatcttaa aaagaactgc tgttgctgtg gcgaataaaa acatttactt
     3841 caagcaaatt gaacttggcc ccatggccaa cttcctttat ctcatcgggg acaaaaacgc
     3901 cggggtttgc gcggtcgtgg atccggcctg ggacattcgt gccgttctgg acgcggcggg
     3961 agtggacgga atgagagtga ccgcggggtt gcttacgcac acgcattttg atcatgccca
     4021 tggcgtggaa gatttgatca aactgaccca gtgtaaaatc tatgtgcatg agcgtgaagc
     4081 caagtattta aaagaatttc aaccacattt gatcgaaacc cgcgaaggaa tgacgatcaa
     4141 aattggagac gtcgaaattc tggttctgca taccccggga cacacggagg gcgggcagtg
     4201 ttttttggta gacggaattt taatttccgg tgataccttg ttcccggggg cctgcggccg
     4261 ctgtgatctt cccggaggga acgaagaagt cctgagaaaa agtctcaaac gtctcgccgg
     4321 gcttgatgag cggatcaagg tttattcagg tcacgcctac ggggccgaat cgagtt
//
LOCUS       DAOSIO010000141         4369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_17727_length_4369_cov_5.481456, whole genome shotgun sequence.
ACCESSION   DAOSIO010000141 DAOSIO010000000
VERSION     DAOSIO010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17727_length_4369_cov_5.481456"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /gene="tgt"
                     /locus_tag="PK590_05830"
     CDS             complement(<1..900)
                     /gene="tgt"
                     /locus_tag="PK590_05830"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOG24149.1"
                     /translation="MKKFGFKLLGKDQKCNARVGRVTTGHGTFETPVFMPVGTQGSVK
                     ALTPRDVKEAGSEIILSNAYHLYIRPGLDVIRKLKGLHKFMGWSGPVLTDSGGYQVFS
                     LSKLRKITEDGVTFNSYFDGRQIHLTPEKVIEIQEALGSDIAMIFDECPAATKDKKII
                     EKAVNVTLRWAKRAKKRHRLRTQALFGIIQGGTFKDLRKQSLERTLEIGFDGYALGGL
                     SVGEPKPEMLECLGEILPLMPPDQPRYLMGVGTPLDFFEAVERGADMFDCVNPTRYGR
                     NGTAFTSDGLVVIRNGKYQKDPKP"
     gene            complement(1123..2166)
                     /gene="queA"
                     /locus_tag="PK590_05835"
     CDS             complement(1123..2166)
                     /gene="queA"
                     /locus_tag="PK590_05835"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOG24150.1"
                     /translation="MKLSDFDYPLPKEKIALFPVNERSDAKLLCVDRRTGAISHRVFR
                     DLSELLGPGDLLVLNNTKVLPARLFGKRATGGKVEALLLREVDENVWECLLKPGGRVK
                     EGQTIDFSSGGIVFLAEVINKPVPDSGQRFIRFSGSGVKEKIRRFGRIPLPPYIDRPD
                     QAVDRSMYQTVFAENEGAVASPTAGLHFDETLLDTIRVKGVEIVPITLHVSYGTFQPI
                     AVESVEDHKMFEEEYEITEDAARKINQVKQQGRRIVVCGTTCVRALESAASDLTTHGV
                     VPGKARTSIFIHPPYAFRMVDALITNFHMPRSTLLVMTAAFLGYDKMMAAYKEALRAD
                     YRFASYGDAMFIS"
     gene            complement(2203..2823)
                     /locus_tag="PK590_05840"
     CDS             complement(2203..2823)
                     /locus_tag="PK590_05840"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HOG24151.1"
                     /translation="MALLPKTGVSPIMTKMEKICVLGSGTVRMNSPVYRQAEELGRAL
                     ARAGFSVCHGGYGGVMEAVAKGCREAGGHNSGVTIAGKRRPSNAKRHGNSLFVASRVP
                     RKNPYTSAEIKVPSWEKRLLKLIEMGDAYVFLDGATGTMNELFFVWEMANRGLHAKPI
                     LLLGKELHQLVKFLKKNGMVKIPENFHRVATIQRVVKYLVERRGRD"
     gene            complement(2839..3537)
                     /locus_tag="PK590_05845"
     CDS             complement(2839..3537)
                     /locus_tag="PK590_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24152.1"
                     /translation="MNTSRVFNMITGGIVAVALIAGFVGVSRWIQRERVLRQVIERLS
                     ADTRVADVLVTKSEYDEASKKIRTTIKFLEYDAQGAPLAPKYFTFQGNIIQFQSLVIR
                     FQDQFIEGGDRLRGKSAYLFMKAFVLDGEKTQEFEITKAGEVPLGYAVYGDKAQNEFQ
                     AELWREFWAHALDPVKRERAGIKNAQIEAPGSLFLPGSIYTIHIEHDGGMRIDTQPVP
                     EILKGEKVETESGA"
     gene            complement(3793..3866)
                     /locus_tag="PK590_05850"
     tRNA            complement(3793..3866)
                     /locus_tag="PK590_05850"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3830..3832),aa:Arg,seq:ccg)
BASE COUNT          991 a         1268 c         1065 g         1045 t
ORIGIN      
        1 cggtttcgga tctttttgat atttcccgtt acgaatcacg acaagcccgt ccgacgtgaa
       61 ggccgtcccg ttccttccgt agcgggtcgg gttcacgcaa tcaaacatgt ccgcgccccg
      121 ctccaccgct tcaaaaaaat caagcggcgt cccgactccc attaaatacc gcggctgatc
      181 cggcggcatt aacggcaaaa tttctccgag acattccagc atctcgggtt tgggttctcc
      241 gacggaaagt ccacccagcg cgtaaccgtc aaatccgatc tcaagcgtcc gctcaagcga
      301 ctgcttgcgg agatccttga acgtccctcc ctggataatg ccaaaaagcg cctgcgtcct
      361 gagccggtgg cgttttttcg ccctcttcgc ccagcgcaga gttacgttaa cggccttttc
      421 gattattttt ttatccttcg tcgcggccgg acattcatca aaaatcatcg cgatatcgct
      481 cccgagcgct tcctggattt cgatcacttt ttcgggggtc aggtgaatct ggcgaccgtc
      541 aaaataagaa ttgaacgtaa caccgtcctc cgtgattttc cggagtttgc tcagggaaaa
      601 aacctgatag ccgccactgt cggtcaggac ggggccactc cagcccatga atttgtggag
      661 ccctttgagt ttgcgaatca cgtcaagccc cggacggata taaaggtggt aggcgtttga
      721 gagaatgatc tccgatcccg cttctttcac gtcgcgcgga gtaagggcct tcacgctccc
      781 ctgggttccg accggcataa agaccggtgt ctcgaatgtc ccgtgccccg tcgtcacccg
      841 cccgactcgc gcgttacatt tttgatcctt gccaagtaat ttaaagccaa actttttcat
      901 aaatcccatt caccgtcatc ataaagaccg ttaatccccg gagcgtccct tcttcctaaa
      961 gacggaccgc cggtttctca gtaggtccga acttcgccct cttcgacttt tcgctaaccc
     1021 aagatcaggg ttgtttctat ctgttgacga aatgccgcgt aagtcgtcac ggaaagtttt
     1081 tccatatttt taccgtggag ccgcgggcgc gcgccgccca gtttatgaaa taaacatggc
     1141 gtcgccatag ctggcgaacc ggtaatcggc gcgcaaggct tccttgtagg ccgccatcat
     1201 tttatcatat ccgaggaagg ccgcggtcat gacaagaagc gtgctccggg gcatatgaaa
     1261 gttggtaatc agggcgtcta ccatcctgaa cgcgtaaggc ggatgaataa aaatcgaggt
     1321 cctggctttt ccgggaacaa caccgtgagt tgtcaaatca gacgcggcgg attcaagcgc
     1381 ccggacacag gttgttccgc agaccacaat gcgccggcct tgttgtttca cctgattaat
     1441 tttccgggcc gcgtcttcgg tgatctcgta ttcttcctca aacattttgt gatcctcgac
     1501 cgattcgacc gctatcggct gaaaggtacc gtaacttacg tgaagtgtga tcggcacgat
     1561 ctcaacccct ttaacgcgga tggtatcgag cagggtctcg tcaaaatgaa gccctgccgt
     1621 cggtgaggcg accgcgccct cgttctcggc aaaaaccgtc tggtacatgc tccggtccac
     1681 cgcctggtcc ggccggtcaa tgtaaggagg taatggaata cgcccaaatc tccggatctt
     1741 ctctttgacc ccggaaccgc taaaccggat aaaccgctga cctgaatccg gcaccggctt
     1801 gttgatgact tcagccaaaa aaacgattcc tcccgagcta aaatcaatcg tttgtccctc
     1861 tttcacccgt ccgccaggct tcaaaagaca ctcccagaca ttctcgtcga cctcccttaa
     1921 aagaagggcc tccaccttgc cgcccgtggc gcgtttccca aaaagacgcg ccggaaggac
     1981 tttggtgtta ttcaggacaa gaagatcccc gggaccgagg agttcggaaa gatcacggaa
     2041 gacacggtgc gagatggctc ccgtccggcg atccacgcaa agaagtttcg cgtccgatct
     2101 ttcgttcacg ggaaacagcg cgatcttttc tttcggaagc gggtaatcga aatcggataa
     2161 tttcataggg tttagtggat gacgtctggc ggacagaatg tttcaatccc gtcccctgcg
     2221 ttctaccaga tatttgacaa ctcgctggat cgtcgcgacc cggtgaaaat tttcgggaat
     2281 ttttaccatt ccatttttct tcagaaattt cacaagctga tggagctctt tgccgagcaa
     2341 aagaatcggc ttcgcgtgaa gtccccggtt ggccatttcc cagacaaaga aaagttcgtt
     2401 catcgttcca gtcgcgccgt ccagaaagac ataagcgtca cccatttcga tcagtttcag
     2461 gagacgcttt tcccaggagg gcaccttgat ttcagcgctg gtgtaaggat ttttacgcgg
     2521 tacacgactg gcaacaaaca acgagtttcc gtgacgcttc gcgttggacg gccgacgctt
     2581 tcccgcgatg gtcaccccgc tattatgccc gccggcttca cgacaccctt tcgccaccgc
     2641 ttccatcact ccgccgtatc cgccgtgaca aacggaaaat ccggcgcgcg ccagcgcccg
     2701 gcccaactcc tcagcctgcc tgtagactgg agaattcatc cggaccgtcc cgctacccag
     2761 aacacatatt ttttccattt tagtcatgat gggagagacg ccagtcttag ggagaagggc
     2821 cattctttcc cctatccgct aagcaccgct ttcggtttca accttttcgc ctttcaaaat
     2881 ctcggggaca ggctgggtat cgattcgcat cccgccgtcg tgctcgatgt ggatcgtgta
     2941 gatcgaaccc ggaaggaaca atgaccccgg cgcttcgatc tgcgcgttct tgattccggc
     3001 gcgttcgcgc ttgacgggat ccagcgcgtg ggcccaaaat tcccgccaaa gctctgcctg
     3061 aaactcgttc tgcgccttgt caccataaac ggcgtagccc aaggggacct cccccgcctt
     3121 cgtgatctca aattcctggg tcttctcgcc gtcgagcacg aaagccttca tgaaaagata
     3181 agcgcttttg ccgcgcaacc ggtcaccgcc ctcgataaat tgatcctgga accggatcac
     3241 cagcgattga aactggataa tgttaccctg aaaggtgaaa tacttcggcg cgagcggcgc
     3301 gccctgggcg tcatattcga gaaacttgat cgtcgttctg atttttttcg acgcttcgtc
     3361 atactcgctt ttggtgacca gcacgtccgc gacgcgcgta tccgccgaaa gtcgctcgat
     3421 gacctggcga agaacccgct cccgctggat ccatcggctc acaccgacaa atccggcgat
     3481 cagcgcgacc gcgacaatgc caccggtgat catattgaaa acgcgggatg tattcatgcc
     3541 actccattct acgacgcaaa aatcgcgccc ccgaaacttt ctcgcgaaag ttaccatcgg
     3601 ggctgaagcc gactggaatc cccgccgatc tgtattctca gatcggacgg gtggcggtcc
     3661 gccattattc ttgtcgatct tttacagaca agaataattt cggacggact ctccagattt
     3721 ggaaccatca aatctggaga gagaaccagc gactttcacc ctttgtgaaa aataacctcg
     3781 gtagcaaaaa atcgcgcccg actggaatcg aaccagcaac cttcgcctcc ggagggcgac
     3841 gctctatcca attgagctac gggcgcaagc aaccatgttt ttttaaatta aagcttgcga
     3901 ccagtcccag cggttagctt tcctgtcaag gaaagctgcg agggacgggc gccctaaacc
     3961 acgggcacac ttgcttttta taagcaagtg ccgtcccgtc actcttcagt gaccaccgtg
     4021 tacgagccgg caagcaacca tgtttttttt aaattaaagc ttgcgactgg cgcagattca
     4081 agattgaaat tgtcggtttg acaatttcaa tgtttcttga tgcgccgtac ccttatcacc
     4141 tatcgtttca gttgcaaaag ggtaccagtc ccaggaatcc ttcaacgatg aaaaacggga
     4201 gggagtataa cagatttttt agtggaggtc tcgggtgagt ttacaaacgc tctggccaga
     4261 tatcccttag caggtaccgt tcgggaggtt aacatagcca agatcattcg acttacccca
     4321 ggtacaatct gccgtcagcg tgatagagga atcccctttt gtggcttgc
//
LOCUS       DAOSIO010000142         4320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_17974_length_4320_cov_5.368581, whole genome shotgun sequence.
ACCESSION   DAOSIO010000142 DAOSIO010000000
VERSION     DAOSIO010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17974_length_4320_cov_5.368581"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..161
                     /locus_tag="PK590_05855"
     CDS             <1..161
                     /locus_tag="PK590_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HOG24153.1"
                     /translation="AKNRISILAPIGMALLGYRVGDIVEWKVPAGTRRLKIEEVLYQP
                     EAAGEYDL"
     gene            232..1683
                     /locus_tag="PK590_05860"
     CDS             232..1683
                     /locus_tag="PK590_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOG24154.1"
                     /translation="MIPFNQRVLCIGFGSVGKCTLPVLLKHIRIPYKNITVLDFEDKR
                     KELAPWIKKGVRFAQERITPVNVTRTLSKYVSRGGMIIDLTWNIDSLDMISWCHENEV
                     LYANTSVEEWDPYANIDKKTPLEKSLYFKQLEIRKMVSKWSDGATTAVLDHGANPGLI
                     SHFTKQGLMDIARKVVRDKTTSKNDRKVLEHLIRDEDFSHLAMKLGVKTIHVSERDTQ
                     ISRRPKEVDEFVGTWSIEGLREEAIAPSEIGWGTHEKEMPSIACVPEYGPKNQIFLSQ
                     MGMNTWVRSWVPNYEIVGMVIRHAEAFTLSERLTVWKNKKPLYRPTVHYAYMPCDETL
                     SSLHELRCRNYELQPKCRIMSDEIISGTDILGALIMGHKYNSWWTGSMLSIEEARKLV
                     PHQNATTIQVAIGVVAAAMWMIENPRKGVCEPEDLPHEQVLEVAKPFLGTFVSEASDW
                     TPFKNYQVFFPENPTSLLDKKNPWCFNNFLFRD"
     gene            1765..2067
                     /locus_tag="PK590_05865"
     CDS             1765..2067
                     /locus_tag="PK590_05865"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOG24155.1"
                     /translation="MSKPSIDPRILERAAKTLKALAHPDRLRIVEELRSRERAVGELT
                     EKLGLAQAIVSKHLAQLKRAGVLESRTDANFRYYSLANPRVMEILNCIKKSCPGGK"
     gene            2064..>4320
                     /locus_tag="PK590_05870"
     CDS             2064..>4320
                     /locus_tag="PK590_05870"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG24156.1"
                     /translation="MNMRVLIVGGVAGGASAAARARRLSEDAEIIMFERGEHISFANC
                     GLPYHIGGAIADRERLIVQTPEATRKTFRIDVRTRTEVFKIDPAKKIIFTRELATGAE
                     REEPYDFLVLSPGAEPARPPLPGIHSSKIMTLRNLADMDAIKKALETLRPERALVVGG
                     GYIGLEMTEALREKQIPVTLVELTPQVMAPADPEMAAPLHQELSLHGVDLRLGTSVKA
                     FREETNGLHAELSTGESLACGLAILAIGVKPETSLAKDAGLKIGPRGGILVDNGMRTS
                     DPSIFAVGDAIEVKDFVTDEPALVPLAGPANRQGRIAADNIFGRNSVYKKTQGTAICK
                     VFDLAIGMTGASEKTLKRLGLPYEKIYVHAASHASYYPNAFPISLKLVFDPVHGKILG
                     AQAVGPDGVDKRIDVIATALRAGLTVYDLEHLELSYAPPYGSAKDPVNYAGFVASNVL
                     RGDVRICHARDLESPEKNQRILDVRPAGATGAGVIPGASHLPLEELRDRLAELSKDKE
                     YLVYCKVGLRGYLACRILTQNGFKCKNLSGGYETYRASLGISSARPAKPEMKTDTNER
                     DERKEPVEKKEPAVTIKEIDACGLQCPGPILRIKTEIEKIRPGESLRISATDPGFAAD
                     VRAWCQSTGHELAEFSSAGKKYHATILKKEPRAVSSVPSSAGPSKKKTIVVFSSDFDK
                     VMAAFVIANGALAMGSEVTMFFTFWGLNALRKPKSGPVKKNFIETMFGFMMPKGAEKL
                     ALSKMNMGGMGL"
BASE COUNT         1053 a         1238 c         1166 g          863 t
ORIGIN      
        1 ccgcgaaaaa cagaatttcc attctcgcgc ccatcggtat ggccctactc ggctatcggg
       61 tgggtgatat cgtcgaatgg aaagtgcccg ccggaacacg gcggcttaaa atcgaggaag
      121 ttctttatca acccgaagcc gccggtgaat atgacctcta agacgacggc aacggggatt
      181 ttgtcgtatc aactgtcgga tgagttttaa acgggaaagg agacaacgcc catgattcct
      241 tttaatcaac gtgtcctttg catcgggttt ggttcggtcg gcaaatgcac cctgccggtc
      301 cttctcaagc acatccgcat tccttataaa aacattaccg ttctggattt cgaagacaag
      361 cggaaagaac tcgcgccctg gatcaaaaaa ggcgtccggt tcgcccagga gcggatcacc
      421 cccgtcaacg tcacccgcac cctgtccaaa tatgtgtccc ggggcggcat gattattgac
      481 ctgacctgga acatcgattc actggacatg atctcctggt gtcacgaaaa cgaagtcctt
      541 tacgcgaata cctcggtgga agaatgggac ccttacgcca atatcgacaa aaagaccccg
      601 cttgaaaaat ctctttattt caagcaactg gagatccgga aaatggtttc caaatggagc
      661 gacggcgcca cgacggccgt ccttgatcac ggcgcgaacc ccgggctgat ctcccatttc
      721 accaagcagg ggctcatgga catcgcccga aaagtggtcc gtgacaagac cacctcgaag
      781 aacgaccgaa aagttctcga acacctgatc cgggacgagg acttctccca cctggccatg
      841 aaacttggcg ttaaaacgat acacgtcagc gagcgagaca cccagatcag ccggcgtccc
      901 aaggaagtcg acgaattcgt cggcacctgg tccatcgaag gattgcgcga agaggcgatc
      961 gcgccttcgg aaatcggctg gggaacacac gaaaaagaga tgccttccat cgcctgtgtt
     1021 ccggaatacg ggccgaaaaa ccagattttc ctttcccaaa tggggatgaa cacctgggtg
     1081 cgttcctggg ttcccaacta cgaaatcgtc gggatggtca tccgtcacgc cgaggccttc
     1141 actctttccg agaggctgac cgtgtggaaa aacaaaaaac ctctttaccg gccgaccgtc
     1201 cactacgcgt acatgccctg cgatgaaacc ttgtcgtcgc tccacgagct ccggtgccgg
     1261 aattacgagc tccagccgaa atgccgaatc atgtccgacg aaatcatttc gggaaccgac
     1321 atcctgggcg cgctgatcat gggacacaag tacaactcct ggtggaccgg aagcatgctc
     1381 tcaatcgaag aagcgcgcaa actcgttccc caccaaaacg cgacgacgat ccaagtcgcc
     1441 atcggggtgg tcgccgcggc gatgtggatg atcgaaaatc cgagaaaagg cgtttgcgaa
     1501 cccgaagact tgccgcacga acaagtcctt gaagtcgcga aaccgttcct cggcaccttt
     1561 gtctcggaag cttccgactg gacccctttc aagaactacc aggtgttctt tccggaaaac
     1621 ccgacttccc tgctggacaa aaagaatccc tggtgcttca acaattttct ctttagggac
     1681 tgatcgcgtc agggattgat ctcgccggac ggtgttcgat aagtattcct tgacgggtat
     1741 attccataaa tggtatattc cgtcatgtcg aaaccgtcca ttgaccccag aatacttgaa
     1801 cgagcggcca aaacgctgaa agcgctcgcg cacccggatc gtctgcggat tgttgaagag
     1861 ctccgctctc gtgagcgcgc cgtcggcgag ctcacggaaa aacttgggct cgcgcaggcc
     1921 atcgtctcga aacacctggc ccaactcaag cgggccgggg tccttgaaag ccgcaccgac
     1981 gccaactttc gatattattc cctggccaac ccaagggtca tggagatctt aaactgcatc
     2041 aagaaaagct gtcccggagg gaaatgaata tgcgagttct gattgtggga ggagtcgccg
     2101 gaggggcctc ggccgccgct cgcgcgcgtc gtctctcgga agacgccgaa attatcatgt
     2161 ttgaacgggg cgaacacatc tcgttcgcga actgcggact cccttatcat atcggcggcg
     2221 ccatcgcgga ccgcgaacgg ctgatcgtcc agaccccgga ggccacgcgg aaaactttca
     2281 gaattgacgt ccgcacccgc accgaagttt tcaaaattga tccggcaaaa aaaatcatct
     2341 tcacgaggga actcgcgact ggcgccgaac gtgaggagcc ttatgatttc ctggtcttga
     2401 gccccggcgc ggaacccgcc cgtccgcccc ttcccggcat tcattcttcc aaaatcatga
     2461 ccttacgaaa tcttgccgac atggacgcga tcaaaaaagc tttggaaact ttacggcccg
     2521 aacgcgcgct cgtcgtgggc ggaggttaca tcgggcttga gatgacggaa gcgttgaggg
     2581 aaaaacaaat tcccgtgaca ctcgtcgaac ttacgcctca ggttatggcc ccggcggatc
     2641 cggaaatggc cgcgccgctc catcaggaac tctcgctcca cggcgtggat cttcggctcg
     2701 gcacctcggt caaggcgttc cgcgaagaaa cgaacgggct tcacgcggag ctcagcaccg
     2761 gcgaatcgct cgcctgcggt ctcgccattc tcgcgatcgg ggtcaaaccg gaaaccagtc
     2821 tcgcgaaaga tgccggcctc aaaatcgggc cgcgcggcgg cattctggtc gataacggaa
     2881 tgcgcacgag cgatccttcc atctttgccg tcggcgacgc gattgaggtg aaagatttcg
     2941 tgacggatga acccgctctt gttccactcg ccggtcccgc gaaccgtcag ggacggatcg
     3001 ccgcggacaa tatttttggc cgaaactccg tttataaaaa aacacaaggg acggccatct
     3061 gcaaagtttt cgatctcgcg atcggcatga ccggcgcgag cgagaagaca ctgaaacggc
     3121 ttggtctccc ttacgaaaag atttatgtcc acgccgcgtc gcacgcgagt tattatccaa
     3181 acgcattccc gatcagtctg aaacttgtct ttgatcccgt tcacggaaaa attctcgggg
     3241 cgcaggcggt cggcccggac ggcgtggaca aacggattga tgtgatcgcg accgctcttc
     3301 gcgcggggct cacggtttat gacctggaac acctggagct ttcctacgcc ccgccctacg
     3361 ggtccgcgaa agatcccgtc aattacgcgg ggttcgtggc ctcaaacgtc ctgcgcgggg
     3421 atgtccggat ttgtcacgcg cgggatcttg aatcgcctga aaaaaatcag cggattcttg
     3481 acgtgcggcc ggcgggagcc acgggggccg gcgtaattcc aggcgcgagt catcttcctc
     3541 ttgaggaact cagggacagg ctcgcggaac tttcaaaaga taaagaatat ctcgtttact
     3601 gcaaggtggg gcttcgcggg tacctcgcct gccgaatcct cacgcaaaac ggttttaaat
     3661 gtaaaaacct gagcggcggt tacgaaacct atcgagccag cctcgggatt tcttccgcgc
     3721 gaccggcaaa accagaaatg aaaaccgaca ccaatgaaag agatgaaaga aaggaacccg
     3781 tggaaaaaaa agaaccggct gtcactataa aagaaatcga cgcgtgcggg ctccagtgcc
     3841 cgggccccat tctcaggatt aaaaccgaga ttgagaaaat ccggcccgga gaatctttgc
     3901 ggatctcggc gacggacccc ggtttcgcgg ccgatgtccg cgcctggtgc cagagtacgg
     3961 gacacgagtt ggccgaattc agctccgctg gaaaaaaata ccacgcgacg atccttaaaa
     4021 aggaaccgcg cgcggtctca agcgttcctt cctccgccgg gccctcaaag aaaaagacga
     4081 tcgtggtctt cagttcggac tttgacaaag tgatggccgc ttttgtcatc gcgaacggcg
     4141 cgctcgcgat gggaagcgag gtcacgatgt tcttcacctt ctgggggttg aacgccctta
     4201 ggaaaccgaa gtccggtccc gtcaaaaaga actttattga aaccatgttc ggtttcatga
     4261 tgccaaaagg agccgagaaa ctcgcgcttt ccaaaatgaa tatgggcggg atggggcttc
//
LOCUS       DAOSIO010000143         4313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_18010_length_4313_cov_4.282292, whole genome shotgun sequence.
ACCESSION   DAOSIO010000143 DAOSIO010000000
VERSION     DAOSIO010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18010_length_4313_cov_4.282292"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PK590_05875"
     CDS             complement(<1..233)
                     /locus_tag="PK590_05875"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685292.1"
                     /note="converts 2-oxoglutarate to glutamate; in
                     Escherichia coli this enzyme plays a role in glutamate
                     synthesis when the cell is under energy restriction; uses
                     NADPH; forms a homohexamer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HOG24157.1"
                     /translation="MAKAGASLSEKEINAFMKKVIARNPGEKEFHQAVREVVESVTPF
                     IEKHPKYKDAKILERMVEPERVIVFRVPWLDDKG"
     gene            685..2004
                     /locus_tag="PK590_05880"
     CDS             685..2004
                     /locus_tag="PK590_05880"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG24158.1"
                     /translation="MNLSWKEGLPAATMQSMVDEYLIPFALFLGASPLGVGFLTAVPN
                     LLGSFSQFFAVHVVQLLGSRLRFLTRGATLQAALLLPVAGLAFMTGSRRLLVLIVLIS
                     LFRIIGSLIGTVWGSLMSDYLKPEERGKYFGWRSSLAGFANIAGIILGGVFLFIFRET
                     SIAFGFALLFTLAALARFLSAILMGRMEDLPQQKNSGDTFTFFMFLKRFRESNFVKFV
                     LYVASITFATNIAAPFFSVYMIRDLHFNYLVFMFVHLGAMTAGLFSFRIWGRHADSVG
                     NARVLKLTSFLIPIIPVLWIFSSNLIYLFTIEIFSGFVWGGFNLCTLNFVYDAVSPGK
                     RIRCLSYFTFINGIATFAGAITGGYLAENLPPFLGSRILTLFLLSASLRALSHFILSK
                     KFHEVRTTPKKVSSVELFFSVVGIRPVLGLGRDWGFMASLRKFPRKD"
     gene            complement(2008..>4313)
                     /locus_tag="PK590_05885"
     CDS             complement(2008..>4313)
                     /locus_tag="PK590_05885"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOG24159.1"
                     /translation="KTQQRYHRQKTKRVYYLSLEFLIGRLLGSYILNLDLWDETEQAL
                     EEFGMDLEEIRNEEVDAGLGNGGLGRLAACFLDSMATLGIPAHGYGIRYDYGIFNQKI
                     VNGYQVEAPDEWLKHGNAWEFARPEYAQQIKFYGNTNMYHDKQGRLRVEWVNTEDVVA
                     MPYDIPVPGFKNDVVNTLRLWSARGSEEFNFQYFNDGDYEHAVYEKMFSENISKVLYP
                     NDSTSMGRELRLKQEYFFTAASIMDIIRRFKIDSVVFQNFPDKAAIQLNDTHPSLAIV
                     EFMRILLDEEQLEWGVAWDLTVRTFAYTNHTVMPEALECWPVPMFEKLLPRHMELVYE
                     INDRFMKELAAKYPGDVDRLRRMSLIEEGNPKRIRMAHLAVIGSHSVNGVSALHSELI
                     KTQLFKDFYEVFPEKFNNKTNGIAHRRWLIKANRPLAKLITDAIGTGWEKDLGGLEKL
                     IPFRHDKLLKKKWRQVKYDNKKVLAEILYKTQGVMVNPDSMFDVQVKRIHEYKRQTLF
                     VLYAISEYLKLRENPDAYMTPRTFIFGGKAAPGYFMAKLIIKFINSVASVVNHDKANK
                     DKLKIVFLENYRVSLAEKVFPASDLSEQISTAGTEASGTGCMKFMMNGALTVGTHDGA
                     NIEIADAVGHENIYMFGLRAHEVAEIKSRGYQPGMYLERSARLQEIFHLIRTNFFSPT
                     ERGIFDPLVQNLTLSDPYMVCADFESYCEIQNQISENYQDQDGWTEKAILNVARSGHF
                     SSDRTIREYSRDIWKVPVRADADTPNP"
BASE COUNT          990 a         1174 c          990 g         1159 t
ORIGIN      
        1 cctttatcat caagccacgg aacccggaac acaatgacgc gttcaggctc aaccattcgc
       61 tcaagaattt tcgcgtcttt gtatttggga tgcttttcga taaacggcgt gacggactcc
      121 acgacctcac gcacagcttg gtggaactct ttctcccccg gattcctcgc gataactttc
      181 ttcatgaacg cgttaatttc tttctccgac aagcttgctc ctgcctttgc cattgtcttc
      241 acctttggtt aaaatgggtg cattgccgcc tcgtcaacgg caacaaattt ctcaaaaaaa
      301 aagccctttt cggcagatct ggctgccaaa aaggactccg ttaccctcaa actacgacct
      361 gatggcccgc caccgacttt gtgacgaaca gcgggattat atcacggcgt ctttcgtttt
      421 caatttgatt ttaagtttcg tcaataggcc gaaaaaacga aaaggcacgg aatgcagcat
      481 aacgctttgc gagttgtttc ggccggacgc ggtgtaacct gcttcccgtt tcgtggatac
      541 cggggggctt gcccgcggaa cttgaaacag ctattaaaat tgaaacgtta taaaaatcat
      601 ccttgaggcc gatacatgtc ctgatcggag caactgatgt caaaaacctt ggaccaattt
      661 cgaaaaaatc cacactttaa aagcttgaat ctttcatgga aggaaggtct cccggccgcc
      721 acgatgcaat ccatggtgga tgaatacctg attccattcg ctcttttcct tggcgcttcc
      781 cctctcggcg tcggattcct gaccgccgtt cccaacctgc tcgggtcttt ttcacaattt
      841 ttcgcggtac acgtcgtcca gcttctcgga agccggctcc gttttctcac gcggggggcg
      901 acgctccagg ccgcgctact ccttcccgtg gcaggactgg ccttcatgac ggggtcacgg
      961 cgcctactgg ttttaatcgt cctgatatcc ttgttccgga ttatcggcag tctgattggg
     1021 accgtctggg gaagtttgat gagtgattat ctaaaaccgg aggaacgcgg aaaatatttc
     1081 ggatggcgct caagccttgc cggcttcgcg aacatcgcgg ggatcatcct ggggggcgtg
     1141 tttcttttca tttttcgaga aacgtcaatc gcttttgggt ttgccctttt gtttaccttg
     1201 gcggccctcg cgcgtttttt atcggctatc ctgatgggcc gtatggagga cctgccgcaa
     1261 cagaaaaatt ccggcgacac gttcactttt tttatgttcc tgaaaaggtt cagggaaagt
     1321 aattttgtga aattcgttct ctacgtcgcg agcatcactt tcgcgaccaa tatcgcggcc
     1381 cctttcttca gcgtgtacat gattcgggat cttcatttca attatcttgt ttttatgttt
     1441 gtccatctgg gcgccatgac ggcgggtctt ttttctttcc ggatttgggg ccggcacgcc
     1501 gattccgttg gaaacgcccg ggtcctgaaa ctcacgagtt ttctgatccc catcatcccg
     1561 gttctctgga ttttttcctc aaacctcatt taccttttca ccatcgaaat attttcgggg
     1621 tttgtctggg gcgggttcaa cctttgcacg ctcaattttg tttacgacgc ggtcagtccg
     1681 gggaaacgga tccgctgtct cagttatttc acttttatta acgggatcgc gacgtttgcc
     1741 ggcgcgatca cgggcgggta cctcgccgaa aaccttcccc cttttctcgg gtctcgaatt
     1801 ctcacgcttt ttctgctgtc ggcgagcctg agggcgttat cgcatttcat tctttcgaaa
     1861 aaatttcatg aggttcggac gacccccaaa aaagtttcaa gcgtggaact ttttttcagc
     1921 gtcgttggaa tccgccccgt gctggggctt gggcgagact ggggatttat ggcaagtttg
     1981 agaaaatttc cccggaaaga ctgatcgcta gggattgggc gtatccgcgt cagcccggac
     2041 cggcaccttc cagatgtcac gggagtattc tcgaatcgtt cgatcgctcg aaaaatgccc
     2101 cgatctcgcg acgttaagaa tggccttctc cgtccacccg tcctggtcct gataattttc
     2161 tgatatctgg ttttgaatct cgcaatagga ttcaaaatcc gcgcaaacca tatagggatc
     2221 ggaaagagtc agattttgga ccagtgggtc aaaaatcccg cgctccgtcg gagaaaagaa
     2281 attggtacgg atcaaatgaa aaatctcctg aagacgcgcc gaccgctcca gatacatacc
     2341 cggttgatac ccccgggatt taatctcggc gacctcatga gcgcgcaagc caaacatata
     2401 aatattttca tgcccgaccg cgtccgcgat ctcgatattc gcgccatcat gcgttccgac
     2461 cgtcagcgcc ccattcatca taaacttcat gcaaccggtc cccgaagcct ccgtccccgc
     2521 cgtcgaaatt tgttccgaaa gatcactcgc ggggaatact ttttcggcca aagagaccct
     2581 gtagttttcg agaaaaacaa tcttgagctt atccttgttg gccttgtcat gattcacgac
     2641 gctcgccacg ctattgataa acttaatgat caatttcgcc atgaaatatc cgggcgcggc
     2701 cttccctcca aaaataaacg tcctcggtgt catatacgcg tcaggattct cgcgaagctt
     2761 aagatattca gaaatggcgt ataagacaaa aagcgtctgg cgcttatatt cgtgtatccg
     2821 tttgacctgg acatcaaaca tggagtccgg attaaccata acgccctggg ttttgtagag
     2881 aatctcggcc aacacttttt tgttatcgta cttcacctgg cgccatttct tttttaaaag
     2941 cttgtcgtgg cgaaacggta tcaacttttc aagtccgccc agatctttct cccaccccgt
     3001 cccaatcgcg tcagtaatca gtttcgccag gggccggttc gccttgatca gccaccggcg
     3061 atgcgcgatc ccgttcgttt tattattgaa cttttcggga aacacctcgt agaaatcctt
     3121 gaagagttgc gttttgatca gttcggaatg aagcgccgag acgccgttca cggaatggga
     3181 cccgataacc gcgaggtgcg ccatccggat ccttttagga tttccttcct cgatcaacga
     3241 catgcgccgc aaccggtcca cgtcccccgg gtacttggcc gccagctcct tcataaatcg
     3301 gtcattgatc tcgtaaacca attccatgtg gcggggcaaa agcttttcga acattggaac
     3361 gggccagcac tccagggcct caggcatcac cgtgtgattg gtgtaggcaa atgtccggac
     3421 cgtcagatcc caggccacgc cccattcgag ttgctcctca tccagcaaaa ttctcataaa
     3481 ttcaacaatc gccagagagg gatgcgtgtc attgagctgg atagccgcct tatcaggaaa
     3541 attctgaaac accacgctgt ctatcttaaa ccgccgaatg atgtccataa tggacgccgc
     3601 cgtaaaaaaa tattcctgtt tcagtcgcag ttcccgcccc atggaagtcg aatcattcgg
     3661 ataaaggacc tttgagatat tttccgaaaa catcttctca taaaccgcgt gttcatagtc
     3721 gccatcattg aaatactgaa aattaaattc ttcagatccg cgggctgacc aaagccgcag
     3781 ggtattgacc acgtcgtttt taaatccggg caccgggata tcgtacggca tggcaaccac
     3841 atcttcggtg ttaacccatt cgacccgaag tctcccctgc ttgtcatggt acatgttggt
     3901 atttccataa aatttgatct gctgcgcgta ttccggccgg gcgaactccc acgcgttgcc
     3961 gtgcttcagc cactcatccg gcgcctcaac ctgatatcca tttacgatct tttgattgaa
     4021 aataccataa tcatagcgga tcccgtatcc atgggccgga ataccaagcg tcgccatgga
     4081 atcaagaaag caggcggcaa gacggcccag tcctccgttc ccgaggccag cgtcaacttc
     4141 ctcattacga atctcctcca gatccatccc gaattcttca agcgcctgtt cagtttcatc
     4201 ccaaagatca agattaagaa tataactgcc aagaagacgg ccgatcaaaa attcgaggga
     4261 caaatagtaa acccgtttag tcttctggcg atggtaccgt tgctgagttt taa
//
LOCUS       DAOSIO010000144         4310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_18037_length_4310_cov_3.474501, whole genome shotgun sequence.
ACCESSION   DAOSIO010000144 DAOSIO010000000
VERSION     DAOSIO010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18037_length_4310_cov_3.474501"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PK590_05890"
     CDS             <1..402
                     /locus_tag="PK590_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG24160.1"
                     /translation="AIKVLVIEDDANYFIFLSEMLAYYRSPSFEVSRARMLQSGLDRL
                     RTGDVELVLLDLTLPDSSGLNTLITVRKSSSLIPVVILTGVDDPALSAQAIGLGAQDY
                     IVKGSFDREQLRGRMLNAIDVFYEMRGADEE"
     gene            392..>1288
                     /locus_tag="PK590_05895"
     CDS             392..>1288
                     /locus_tag="PK590_05895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG24161.1"
                     /translation="MKNKVVRILVIEDDPNYFVLLNERLSQCREPAFEVFRAKMLQSG
                     LERLNQSDIQLVLLDLTLPDSAGLDTVVAVRKAVPLMPVIILTGVDDSELAAKAISLG
                     AQDYLVKGSFDRELLNKSILYALERHESQRELQKYYQENQESQRTLVDKEERFKSLVS
                     NIPGAVYRYSLNTSGTWDIEFISEAIYDIVGIRAKEFVGQSIQKYFDLILPQDREMVR
                     EAFNRSVQSASVFNVDYRIFKSEKDFRWVHEQGRGILGEGGKVTHVDGVMFDVTERTK
                     EKERFHQLVYYDALTGLPNRTLF"
     assembly_gap    1289..1298
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1299..2570
                     /locus_tag="PK590_05900"
     CDS             <1299..2570
                     /locus_tag="PK590_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOG24162.1"
                     /translation="FMDRLDQSVKEVKRKKESGAVLIVDLDHFKRINDTLGHGIGDQL
                     IKAVSVRLQKIMYESDTISRIGGGSFMFLLPKVAKSEHAENVANKILTAFKSPFRVQE
                     HDLFTTCSVGIALFPQDGEDPQSLIKNADAAMHLAKDRGKDRYQLYSSSIANNSFERM
                     VLENSLRRALEKDEFRLHYQPQLDLRTGKVVGVETLVRWQHPDLGSIPPLEFIPIAEE
                     TGLIHPIGEWILRTACEQKSIWQKAGFRQLRVSVNLSARQFHYANMVDMVMGILRETG
                     IDPKSLDLEITESTIMERLEETTETLRRLKEMGCHITIDDFGTGYSSLTYLKTFPINM
                     LKIDKSFVDNIVTDADDRAITQAIISMAHSLKLEVVAEGVEEESQLEILKMQGCDILQ
                     GFLFSKPLPADDVTRVIEQTAEKVSPSQPQS"
     gene            2717..4057
                     /locus_tag="PK590_05905"
     CDS             2717..4057
                     /locus_tag="PK590_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOG24163.1"
                     /translation="MYNSAMGNYRFICSHCGKSFESPASEVKECPFCFWSSSVKREDE
                     LAVEKKRLSRTAGGKTGIGSYNPLYFAFKVLLALALVGSVGYFGYLGYRQWDQNQKKT
                     GSFFSVQGEKGETSKAEREVPDTAVSELSSQERDLLLREVSLPERAVASEAEKIILAR
                     EVKFKTGWSDQLPSPAWSLAQYEQMIAEQERFYKITLPRSYKKKLTGLFENKYLAGVG
                     AFAKGDLLATRDLFIESLLFPPYSTNVQKHRGVALTMLKPFITDTLSKIGALNQMVAD
                     QNRRQQGKEIEAEYGSLFALIQEGRWEEAILKLDQLQLVATQMQSLTQERPAMPPYPS
                     VVGMIDQDIQRSLAALSTPGVSSTTDLLHVREDLAQKKDVLVDLSDEAYNKAIAIYQE
                     GMTFVREGKYKDAFRVLSTIRGPETLRNQAQTKLAILAKVVSLPESSETRSRDV"
     gene            4054..>4310
                     /locus_tag="PK590_05910"
     CDS             4054..>4310
                     /locus_tag="PK590_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24164.1"
                     /translation="MSDDTLVFGVVQSGRFVYGLVRDLAMAKQLVKCASRFGIRARNF
                     DRADSLMKSVEETKPFLVILDFENLECESYKTLKEIRESADA"
BASE COUNT         1075 a          929 c         1161 g         1135 t
ORIGIN      
        1 gcaattaaag ttttagtgat tgaggatgat gccaactact ttattttttt gagcgagatg
       61 ctggcgtatt atcgaagccc ttctttcgaa gtcagccgcg caagaatgct ccaatccggg
      121 ctcgatcgtc tcaggacagg ggatgttgag cttgtcctcc tggatttgac tctgccggat
      181 agctctggat taaacacctt gattaccgtt agaaaatcgt catctctcat accggttgtt
      241 attctgactg gtgttgatga ccccgccctt tcggcccagg ctattggcct aggcgcgcag
      301 gactacattg taaaggggag ctttgaccgg gagcagttga ggggtagaat gctcaacgca
      361 atagatgtgt tttatgaaat gagaggcgct gatgaagaat aaagtggtac ggatattggt
      421 gattgaggac gatccgaatt attttgtcct tttgaacgag cggctcagtc agtgccgtga
      481 accggcattt gaagtttttc gtgccaaaat gcttcaatcg ggtcttgaac ggctgaacca
      541 gtcggatatt caacttgttc ttctcgatct cacgctccct gatagcgccg gactagacac
      601 cgtggttgct gtccgtaagg cggttcctct tatgcccgtt atcattctta ccggagtgga
      661 tgattcggag cttgcggcaa aagcgattag cctaggagcc caggattatc ttgtgaaggg
      721 aagttttgac cgggagcttc tcaacaagag catcctttac gcgctggaac ggcacgagtc
      781 ccagcgtgag ctccagaaat attatcagga aaatcaggaa tctcagcgta cgcttgttga
      841 taaggaagag cgctttaaga gtcttgtttc caacattccc ggcgcggttt atcggtacag
      901 tcttaacact tccgggacct gggacatcga gtttatcagt gaggcgattt atgatatcgt
      961 ggggatacgt gccaaagagt ttgtggggca gagcatccag aaatattttg acttaattct
     1021 gcctcaagat cgtgaaatgg tgagggaggc ttttaaccgt tctgtccagt ctgcctcggt
     1081 gtttaatgtg gattaccgga tttttaaatc ggaaaaagat tttcggtggg tccatgaaca
     1141 ggggcgcggt atacttggcg aagggggcaa agtgacccat gtggatggag tgatgtttga
     1201 cgttacggaa cgcaccaaag aaaaagaaag attccatcag ctggtctact atgacgctct
     1261 gacggggctg cccaaccgga ccctgttcnn nnnnnnnntt tatggatcgt ctggatcaat
     1321 cagttaagga agtcaagcgg aagaaagaat cgggagcggt actgattgtt gacctcgacc
     1381 attttaagcg aatcaatgat actctcggac atggcattgg cgaccaactg atcaaggcgg
     1441 tatcggtgcg cctccagaaa attatgtatg aatcggatac gatttcgaga atcgggggtg
     1501 gaagcttcat gtttctgctt cccaaggtcg ccaagtccga gcacgccgaa aacgtagcca
     1561 acaagattct gacagctttc aaatcgccgt ttcgcgttca ggagcatgac cttttcacga
     1621 cttgcagtgt ggggattgct cttttcccgc aggacgggga agatccccag tctttgatta
     1681 aaaatgcgga tgcggccatg catttggcca aggatcgcgg caaggaccgt tatcagctct
     1741 attcttcttc gatcgctaac aacagttttg aaaggatggt tctcgagaac agcttacgcc
     1801 gagctctcga gaaggatgaa ttcaggcttc attatcaacc gcaactggat ctccgaaccg
     1861 ggaaggttgt tggagtggag accttggtac gttggcaaca tccggatctt gggtcgatac
     1921 cgcctcttga gtttattcca attgccgagg aaacgggtct tatacatccg atcggtgagt
     1981 ggattcttcg gacggcatgt gaacagaaaa gcatttggca gaaggcggga tttcgccaac
     2041 tgcgggtatc cgtcaatctt tcggcccggc agtttcatta tgcgaacatg gttgacatgg
     2101 tcatgggtat tcttcgagaa acgggaattg atcccaagtc gctggatctg gagattacgg
     2161 aaagtacgat catggaacgc cttgaggaga caacggaaac cctgcggcgg ctcaaggaaa
     2221 tgggatgtca tatcaccatt gatgattttg gaaccgggta ttcttccctc acgtacctca
     2281 agacttttcc gatcaacatg ctcaaaattg ataaatcctt tgtggataat attgtcactg
     2341 atgcggatga tcgggccatc acgcaagcca ttatttcgat ggcgcacagc ctgaagcttg
     2401 aggtggtagc ggaaggggtg gaagaagaat ctcaactgga gattttaaaa atgcagggtt
     2461 gtgacattct ccaggggttt ttgttcagta agccactgcc ggcggatgat gttacgcgag
     2521 ttattgagca gacggctgag aaggtatccc ctagtcaacc gcaatcttga tttcgaatct
     2581 gtccgtttta ggtcgcgtta atgcctagcc tctgtaaaaa agatcgctcc tccgtcgcgc
     2641 gccaggtttt taaaatctca ccatgggaaa gtgatttttt ctccccgaca ggatcactgg
     2701 cataaaaaaa cggtttgtgt ataattctgc catgggaaat taccgtttta tctgcagtca
     2761 ttgtggcaaa tcttttgaat ctcccgcctc agaggtgaaa gaatgcccgt tttgtttctg
     2821 gtcatcatcc gttaaaaggg aagatgagct ggccgtcgaa aagaagcgat tatcccggac
     2881 ggctggaggc aagacgggga ttggaagtta caaccccctc tattttgcgt tcaaggtttt
     2941 actcgcgctt gcgttggttg gctccgtggg gtatttcgga tatttgggtt atcgacaatg
     3001 ggaccagaat cagaaaaaga caggatcttt tttttcggtt cagggggaaa aaggggaaac
     3061 ttcaaaagcg gagagagaag ttcctgacac tgcagtgtct gagctgtcgt ctcaggaaag
     3121 agatttgttg ctgcgtgaag tttcattgcc cgaaagagcc gtcgctagtg aagccgaaaa
     3181 aattattctt gccagggaag ttaagtttaa aaccgggtgg tctgatcagc ttccttctcc
     3241 agcatggtct ctcgcgcaat atgagcaaat gatcgcggaa caggaacggt tttacaagat
     3301 tacgcttccc cgaagttata aaaagaaact gaccggactt tttgaaaata aatacctggc
     3361 gggggtgggg gcatttgcca aaggagacct tctcgctacc cgtgatcttt ttattgagtc
     3421 gcttcttttc ccgccttatt caaccaatgt tcagaaacat cgcggcgtgg cgcttacgat
     3481 gttgaagcct tttattacgg atacgctttc caagattggt gccttgaatc aaatggttgc
     3541 cgatcaaaat aggcgacagc aaggaaaaga gatcgaggcc gaatatggat cgcttttcgc
     3601 gttgatccag gaaggccggt gggaggaagc cattctcaaa ctggatcagc ttcagcttgt
     3661 tgcgactcag atgcagagtc ttacgcagga gcggcccgcc atgccaccct acccttcagt
     3721 ggtcgggatg attgatcagg atatccagcg aagccttgcc gcgctttcga ctccgggagt
     3781 ctcgtcaacg acggaccttt tacacgtccg ggaagatctc gctcaaaaaa aggatgttct
     3841 agtggacctt tcggatgagg cgtacaataa agcgattgcg atttaccagg aggggatgac
     3901 ttttgttcgt gaagggaaat acaaggatgc attccgtgtt ttgagtacca ttcgaggtcc
     3961 ggaaacgtta cgcaatcagg cgcagacgaa attggcgatc ctcgcaaaag tagtttcgct
     4021 tccggaatct tcagaaaccc ggagccgcga tgtatgagcg atgataccct ggtttttggt
     4081 gtggttcagt ccgggagatt tgtctacggc cttgtacgag atctggccat ggcgaagcaa
     4141 cttgtgaaat gcgcgtcgcg tttcgggatt cgcgccagaa attttgatcg cgcggattct
     4201 ttgatgaaaa gcgtggagga aacaaagccg tttcttgtga tccttgattt tgagaatctg
     4261 gaatgcgaat cctataaaac tctcaaagag atacgcgaaa gtgccgatgc
//
LOCUS       DAOSIO010000145         4300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_18091_length_4300_cov_3.736160, whole genome shotgun sequence.
ACCESSION   DAOSIO010000145 DAOSIO010000000
VERSION     DAOSIO010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18091_length_4300_cov_3.736160"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2422)
                     /locus_tag="PK590_05915"
     CDS             complement(<1..2422)
                     /locus_tag="PK590_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24165.1"
                     /translation="MIRKGTAIFVCAAFLATSVYLPRARAEISPALMTGYRSLADDLT
                     GSIDLPKKMGKIEEIYRGTGDKIVVLIQDAHSIPDAQRSIQSAIDYFQTQYGISLVGL
                     EGASEKLDPQIFRSFPDKELLRKTFDAYSQRGELTGGTAAALFNTSASAYYGIEDWSL
                     YEAGVSYFLEATAMEGEIEKFLEPMEAALTREKEAVYSSALLEIDRSLADFDRNQIDL
                     AQVLGKLAEYQPPPQGSELAILLEEIRQSQMTDTPVEIEIKKIAGQIFSALKNQPLNP
                     EARRELQEFNGNFQEFRTGRTTPQAFAFYLRELAKKHGVRVKVSRRLAYLTGHQKRLK
                     DIEGTRLFGEFRRYANSVKESLFQNDRQKALDIKTRGLDLMKRLARLELSFEDWEKVQ
                     KLTLRPDQWTATQDPVVSREEISILLNKMEPHLAFYHVAEKRDKALLENIRSMMTKAK
                     QESSLVVAGGFHTEGLTRELKKKGISYVLVMPRIGSIPEEPLYREHMRGQVSWSDYFE
                     VKDGKVNLYDAFVRATRDKLLGDKNALATGGKEWRDQIIRDLASEGRITEAGDYTRFI
                     DERTGTPNLELQALREQWLANIDRFGEGLRKLHAEGNLSESSIMQLIKTITIPEVSLA
                     NQLAPGVQAEVRLLPLIQGPGSSSIARRERPETRRSDTRRKYKVFSGIWSPDTGQLFD
                     EESFQKFLDIIREQKLRADKEISEDSDRKAKWLEIFNAGNRDQLVNTLSSHFNESGQL
                     VSTGENPQLFDDVFINKSFVGKIMFLFVQHYKNTVYKGDSSTLEKLFERLRQKAFEAK
                     TFDGFLREV"
     gene            complement(2615..3532)
                     /locus_tag="PK590_05920"
     CDS             complement(2615..3532)
                     /locus_tag="PK590_05920"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOG24166.1"
                     /translation="MIPFNFHHLYYFYIVAKERSFSRAARILCISQPALSAQIRQFES
                     YWNFRLLDREGRQVTLTEEGALLFNSAKAIFDLSQELADGMSKRKYRIASKIQLGVSS
                     AVPKEIVERLVRFILQADPDAHLTLRRDRIETMVEDLMVHKLDLILNDFAYPSPFEEN
                     IQNHLIATVPMVFCASKKLARKLRDLPEGLDGAPLIVPTAPDQTYQAVQNYIHEHGVK
                     PKVVAEIQDLEMARTLVAMGKGIGLFNSYSVEQSPGRKDLVVLRDHSRHKITDAIYLI
                     RRERKIPHPLVSKILEQFPDAVRREALAS"
     gene            3637..4149
                     /locus_tag="PK590_05925"
     CDS             3637..4149
                     /locus_tag="PK590_05925"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HOG24167.1"
                     /translation="MLQAFEDRLKSIADGDLADFHEKIRERAGKKATAILEKSLKNLI
                     LMTPRLLQRSFHHSARTEVPSSIKNLTGFALTYFYHPKDFIPEDNQHLFGYLDDAYYV
                     GLLCERILKSLARARLAVSGFDRTFLKQFGLAKRSIRLVIPNEAEQIEQMINRIQAGE
                     ETAMANAFES"
BASE COUNT         1026 a         1098 c         1089 g         1087 t
ORIGIN      
        1 taacttcgcg gagaaagcca tcaaatgtct ttgcttcaaa agctttttgt ctcagccgct
       61 caaaaagctt ctctaaggtt gaagaatcgc ctttgtacac cgtgttttta taatgctgaa
      121 caaaaagaaa cattatcttt ccaacaaaag acttattaat aaagacatca tcaaaaagtt
      181 gcgggttctc accagtcgaa accagttgcc ccgattcatt aaaatgagaa gacaaggtat
      241 tcacaagctg atctcgattt ccggcgttaa agatttcaag ccacttagct ttacgatcac
      301 tgtcctccga tatttccttg tcagcgcgaa gtttttgttc ccggataata tcgagaaact
      361 tttgaaaaga ttcctcgtca aataattgac ctgtatctgg ggaccatata cccgaaaaaa
      421 ctttatattt tcgtcgggtg tcggagcggc gggtttcagg gcgctcccgc cgcgctatgc
      481 tggagcttcc tggaccttga attaagggaa gcaatcggac ctctgcctgt actccgggcg
      541 caagctgatt tgctaaagaa acctcaggaa tggtgatggt cttgataagt tgcatgatgc
      601 tggattcgct gaggttgcct tcggcatgga gtttcctgag accttcgccg aagcggtcga
      661 tgttggcgag ccactgttcg cgcaaggcct ggagttcaag attgggagtc cccgtccgct
      721 cgtcaatgaa gcgggtgtaa tcgccggctt cggtaattct cccctcgctg gcgaggtcac
      781 ggatgatctg gtctctccat tcttttccac ctgtagcaag agcgttttta tctcccagca
      841 gtttgtctct cgtggccctt acgaaggcat cgtaaagatt gaccttcccg tctttgacct
      901 caaagtaatc gctccaggaa acttgtcccc gcatatgctc acggtaaagg ggttcttcgg
      961 ggatcgagcc gatccggggc atcacgagga cataagagat tccttttttc tttagttccc
     1021 gggtgagacc ctccgtgtga aaacctccgg ccacaaccag ggagctttcc tgtttcgctt
     1081 ttgtcatcat ggaccggatg ttttcaagga gcgctttatc gcgtttttcc gcgacgtgat
     1141 aaaaagccag atgcggttcc attttgttga ggaggatcga aatttcctca cggctgacaa
     1201 ccggatcttg agttgccgtc cactgatccg gccgaagcgt caacttctga accttctccc
     1261 agtcctcaaa gctgagttca aggcgggcaa gacgcttcat gagatcaagc ccgcgtgttt
     1321 ttatgtccag tgccttttgc cggtcgtttt gaaaaagtga ttctttgact gaattcgcgt
     1381 accgcctgaa ctccccaaac agccgtgtgc cttcgatatc cttcaggcgt ttttggtgcc
     1441 cggtcaaata agccagcctg cgggagacct tgacccgaac cccgtgtttt ttagccagtt
     1501 ccctgagata gaaggcaaag gcctggggcg ttgttctgcc tgtcctgaac tcctgaaagt
     1561 ttccgttgaa ttcctgtagc tcccgccgcg cctccggatt caacggctgg tttttcaaag
     1621 cagaaaatat ctgacccgcg atctttttga tctcaatttc aacgggcgta tccgtcattt
     1681 ggctttgccg gatttcttca agcagtatcg cgagttcgga gccttggggc ggcggctggt
     1741 attccgcgag cttgcccagg acctgcgcga gatcgatttg gttcctgtcg aagtccgcga
     1801 gtgatcggtc aatctcaagg agcgcgctgg aataaacggc ctctttttca cgggtgagcg
     1861 cggcttccat cggctcaagg aacttttcga tctcgccttc catcgcggtc gcctcgagga
     1921 aatacgagac acccgcttca tagagggacc agtcttctat gccgtagtag gcgctcgcgg
     1981 atgtattgaa taatgccgcg gctgtgcccc cggtaagctc acctctttgg ctatatgcat
     2041 cgaatgtctt gcggagaagt tctttatccg gaaaactcct gaagatttgc gggtccagct
     2101 tttccgaagc cccttcgagc ccgacaagag agatgccgta ttgtgtctga aaataatcaa
     2161 tggcagactg gatacttcgc tgggcatcgg gtatcgagtg agcgtcctga atcaggacca
     2221 cgattttgtc gcccgtacca cggtagattt cctcgatctt ccccatcttt tttggtaaat
     2281 caatcgagcc agtcaagtca tccgctaggg aacggtaacc agtcatcagg gccggtgaaa
     2341 tttccgctct cgcccgcggc aggtatacgg atgtcgcgag gaacgccgcg cacacaaata
     2401 tagcggttcc ttttcttatc atcttttcca aaggcttctt ataagtcttt atcatgtgtt
     2461 aacccgatgg tcagaatatt ccccctacaa tattatccta ttattattaa aaaataatcg
     2521 attgctatat ataactatat cttactatat atagttattg aacttgaagc cgtcagaggg
     2581 gagagattaa caaacggtgg atttaacaac ggattcagga agccaacgcc tctctgcgaa
     2641 cggcgtctgg gaactgctcg aggattttgc tgacaagcgg atggggaatt ttccgttcgc
     2701 ggcggatgag atagatcgcg tccgtgattt tgtggcggga gtggtccctg agcaccacca
     2761 ggtctttccg tccgggagat tgttcgaccg agtaagaatt aaaaagtcca atcccctttc
     2821 ccatcgccac cagggtccgc gccatttcca ggtcctggat ttccgccaca accttcggct
     2881 tgaccccgtg ttcgtggata taattctgaa cggcctgata cgtctggtcc ggcgccgtcg
     2941 gaacgatcag aggcgccccg tccagccctt ccggaaggtc gcgaagtttg cgagccagtt
     3001 ttttactggc gcaaaaaacc atcgggaccg tcgcgatcag gtggttctgg atgttttctt
     3061 caaacgggga cgggtaagcg aaatcgttca aaatgaggtc aagcttgtgg accatcaggt
     3121 cctcgaccat cgtctcaatc cggtcccgcc ggagtgtcaa atgcgcgtcc gggtccgcct
     3181 gaaggataaa acgcacgagc cgttccacaa tctccttggg caccgcgctg gaaacaccga
     3241 gctggatttt tgaagcgatc cggtacttgc gcttcgacat cccgtcggcc aactcttgcg
     3301 aaaggtcaaa gatcgctttg gcggaattaa aaagcaaagc accttcttcc gtcagggtca
     3361 cctgccgccc ttcgcggtcg agaagcctga agttccagta gctttcaaac tgtctgatct
     3421 gggcgctaag ggcgggctgt gaaatacaaa gaatccgggc cgcccgggaa aaagagcgtt
     3481 ccttggccac gatatagaaa tagtaaaggt gatggaagtt aaaagggatc ataactataa
     3541 gttatatgaa tgatagaaag tatatatttt atttaatccc aagtctagcg gatacttgcg
     3601 tgttcgcgtg ataaataaaa gatgcggaga gacatcatgt tgcaggcgtt tgaggaccgg
     3661 ttgaaaagca tcgccgacgg ggacctcgcg gattttcacg agaaaatccg cgaaagggcc
     3721 gggaaaaagg ccaccgcgat tttggaaaaa agcctgaaga acctgattct catgacaccc
     3781 agacttcttc agagatcgtt ccaccacagc gctcgaacgg aagtcccctc cagcatcaaa
     3841 aatctgacgg gattcgccct gacctatttt tatcatccga aagattttat cccggaagac
     3901 aatcagcatc tgtttggcta cctcgacgac gcttattacg tcgggctgtt gtgcgaacgt
     3961 atcctgaagt ccctggcccg cgcccgcttg gcggtgtccg gtttcgaccg gacatttctg
     4021 aagcagttcg gcctggcaaa acgcagtatt cggctggtca ttccgaacga ggccgaacaa
     4081 attgaacaaa tgattaaccg tatccaggcg ggtgaagaaa cggccatggc gaacgccttc
     4141 gaaagctgag cccggccgcc acgaataatc ctcgaagagg agaaaaacca tgagcaagaa
     4201 aaaaatctat atcacgggaa aggacgctga gaagctccgc gaactggtcc agagcggggc
     4261 cgtcgggggc gaaaacggaa agttttatct cgaccagctc
//
LOCUS       DAOSIO010000146         4296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_18113_length_4296_cov_4.047394, whole genome shotgun sequence.
ACCESSION   DAOSIO010000146 DAOSIO010000000
VERSION     DAOSIO010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18113_length_4296_cov_4.047394"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /gene="mnmA"
                     /locus_tag="PK590_05930"
     CDS             complement(<1..894)
                     /gene="mnmA"
                     /locus_tag="PK590_05930"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF001138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOG24168.1"
                     /translation="MVGMSGGVDSSMALLLLIKQGWRPVGVSLKYPVWKDPANCLREN
                     VCCSEESFRIARDVCDKLGVPYFIYDVSRKFKKAVIDYFLGRLRLGKTPNPCVMCNRF
                     LKFKELFRWGSRHGIEYVATGHYARVRRNPGLRKYELLKARDTEKDQTYSLSFLDQSQ
                     LARLVLPLGHLRKREIYRMAEKAGFEIFLKQKQSQDFCFVSGKAMNAFLAKKIGLKPG
                     DMLNDEGKIIGRHRGLHFYTIGQRKGLGLSGGPYFVRGHEMGRNTLEITKDEKRLFQK
                     EIVLKPCHFPSAAPKKALRVQA"
     gene            complement(937..1632)
                     /locus_tag="PK590_05935"
     CDS             complement(937..1632)
                     /locus_tag="PK590_05935"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOG24169.1"
                     /translation="MKKYIPVLAYHSFDTKRFPQNKLAIDPELFSRQMDWLRAGRYEL
                     IPLSACAKAKGVSSVFDKKVALTFDDGYLDNYACAYVTLRHLGFPGTFFVTPETIGNQ
                     GFMTWQMVNEMARTPGIEIGSHGLFHKPLADISGPDALRSIVESKKQIEDKIGVPVRA
                     FSYPSGSFTETIVEYVKKAGYEYACAASHVHDRKYLRNPYLIRRVKISSSSATRSSFA
                     LRTSGFYHLLGRP"
     gene            complement(1629..2744)
                     /locus_tag="PK590_05940"
     CDS             complement(1629..2744)
                     /locus_tag="PK590_05940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG24170.1"
                     /translation="MKILHITTHLNTGGITTYLQALVKEQTRAGHEVYLWASSGVCLD
                     DFRSMTREVVSDVPRCKSELSPRLWSQLPRLVSYVRKQKIDVIHTHTRVAQVLVAVSK
                     FFTRVPFLSTAHMFYKKQLGRILYPCWGNAIIANSKTMKDGLIDIFGEKNLPPVTEVV
                     NGIDVEGLRMRVKQVDRRMCRKDYGFCDEEIVILSLSRLIPVKGVHFLIEAFALVARE
                     NPSLRLLVAGSGEPDYKQRLEDQVNALGIREKVRFLGNVTQTEKVFAAADLFVAPYLW
                     PEAFGLSILEAMAAGLPVAGSRSGGIGELLGQGEYGLLFDEKDVKGLSKCLEVYARDP
                     DLRLRMARAGHQAAFRYTALNMYQQTQNVYEQVVGQG"
     gene            complement(2833..3273)
                     /gene="mscL"
                     /locus_tag="PK590_05945"
     CDS             complement(2833..3273)
                     /gene="mscL"
                     /locus_tag="PK590_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOG24171.1"
                     /translation="MKFVDDFKQFIMRGNVVDMAVGVIVGGAFGKIVSSLVNDVIMPP
                     IGLLLGGVDFSSLFLSLNGQVYASLTEAQQAGAPTLNYGVFLNTVINFLIVAFVIFVV
                     IRQISALKRKPEPAPAVPTTKECPFCFSTVSLKATRCPNCTSQI"
     gene            complement(3301..3681)
                     /locus_tag="PK590_05950"
     CDS             complement(3301..3681)
                     /locus_tag="PK590_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24172.1"
                     /translation="MRRKTLQILLVVLGLLTVMNGCTRKVDSERSIDKIKKDIETMSV
                     AELEDYAMAYVSAIQSQRAQIQKIQEKIRKVPIEKFFSNQALQNDIKKVGRKAEALYV
                     RYLLYVTALKQKGGDVTKVQLNPV"
     gene            complement(3725..4084)
                     /locus_tag="PK590_05955"
     CDS             complement(3725..4084)
                     /locus_tag="PK590_05955"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOG24173.1"
                     /translation="MLKKVWKKEKRLSERLPVYHLAKYRNILDAQHAFVVCTVKDISE
                     VGIRCYVKEAIPKGSVLELQIQFPFLTNPETCTASVIRCQKRDSKGQRYEIGLKFNGI
                     DDFLTRALAKKKDLGAT"
BASE COUNT         1150 a         1179 c          900 g         1067 t
ORIGIN      
        1 cgcctgaacc cgcaaagcct tcttgggagc cgccgacgga aaatggcagg gctttagaac
       61 tatttctttt tggaataaac gtttttcatc ttttgtaatt tcgagcgtgt tccgccccat
      121 ctcatgtcct ctgacgaaat acggccctcc cgaaagcccc aagccttttc gttgtcctat
      181 cgtgtaaaaa tgaagtcccc ggtgccgccc aataatcttt ccctcgtcat tcagcatgtc
      241 cccaggtttc aggccaatct tcttggcaag aaaagcattc atcgcttttc ccgaaacaaa
      301 acaaaaatcc tgactttgct tttgcttcag aaaaatttca aatcccgcct tttcagccat
      361 gcgataaatc tcccttttcc ttaagtgacc gagcgggaga acaagccgcg cgagttgcga
      421 ctgatcaaga aaactaagcg aataagtttg atccttctcg gtatcacgag ccttcagcag
      481 ttcatatttt ctaagccccg gatttctccg gactcgagcg taatgtcccg tagccacata
      541 ctcaatcccg tgcctgctcc cccatcgaaa aagctccttg aattttaaaa accgattaca
      601 catcacacag ggattcgggg tcttgcccaa acgcaacctc ccgagaaaat aatcaatgac
      661 cgccttttta aactttcggg aaacgtcata aataaaataa ggaactccga gcttgtcgca
      721 aacatccctg gcgatccgaa aagactcttc actacaacag acattttctc tcaggcagtt
      781 cgcggggtct ttccagacgg ggtatttgag tgacactccc acgggccgcc agccctgttt
      841 tatcagaagt aagagagcca tggacgaatc cacgcctccc gacatgccta ccataatttt
      901 tcggcctctt ttgcctgtga ttttgggaac cattgtttaa ggccgtccca acaggtgata
      961 aaaacccgaa gttcgtaacg caaaagaact tcgagtggcg gaagaagagg agattttaac
     1021 ccgccggatg aggtaaggat tccggaggta tttccggtcg tgcacgtgac ttgcggcaca
     1081 ggcatactcg taaccggctt tcttgacgta ctccacgatc gtctccgtga aagatccgga
     1141 cggataagaa aaggcgcgaa ccggcacgcc tattttgtct tcaatttgtt ttttagattc
     1201 cacaatcgat cgcagggcgt caggccctga aatgtccgca agaggcttat gaaaaagccc
     1261 gtggcttcca atctcgatac cgggcgtacg cgccatttca ttcaccattt gccacgtcat
     1321 aaatccttgg ttacctatcg tttcaggcgt cacaaaaaaa gttccgggaa atcccagatg
     1381 gcggagcgtc acatacgcgc acgcgtaatt gtcaaggtat ccgtcatcaa aagtaagcgc
     1441 aacttttttg tcaaaaacac tagacacccc tttggctttg gcgcacgctg agagcggaat
     1501 taattcatag cgccccgccc tcaaccagtc catttggcgg gaaaaaagtt cgggatcaat
     1561 cgcaagttta ttttggggga atcgcttcgt atcaaaactg tggtacgcca gcacaggaat
     1621 atattttttc atccttgccc caccacttgc tcatacacat tctgggtttg ttgatacatg
     1681 ttcagcgcgg tataccgaaa agcggcctga tgccccgcac gggccatcct caaacgaaga
     1741 tccgggtccc gcgcataaac ctcaagacat tttgacagcc ctttcacgtc cttttcatca
     1801 aaaagaagtc cgtattcgcc ctgccccaaa agttccccaa tccctccgga acgggaccca
     1861 gccacaggaa gcccggccgc catagcttct aaaatcgata acccaaatgc ttccggccaa
     1921 aggtaggggg ccacaaacag gtcagccgcg gcaaaaacct tttcggtctg cgtgacattt
     1981 cccaaaaacc ggaccttctc ccgaatgccg agggcgttca cctgatcttc aagccgttgt
     2041 ttataatccg gctcacccga tcccgccaca agaagtctta aagacggatt ctcccgggca
     2101 accagcgcga acgcctcaat caaaaagtgg acgcctttga caggtattaa acgggacagg
     2161 gagagaataa cgatttcttc gtcacaaaac ccatagtcct tccggcacat cctccggtca
     2221 acttgtttca ccctcatcct taaaccttca acgtcaatgc cgttaaccac ctccgttaca
     2281 ggaggcagat ttttttcgcc aaaaatatca atcaatccat ccttcatcgt ttttgaatta
     2341 gcgatgatgg cgtttcccca gcagggataa agaatccgtc ccagctgttt tttataaaac
     2401 atgtgcgctg tcgagagaaa aggcacacgc gtgaagaact tcgacaccgc gaccaacact
     2461 tgagcgaccc ttgtgtgtgt gtggataaca tcaatttttt gcttcctcac ataggaaacc
     2521 aatcgcggaa gctgagacca gagtctcggg gaaagctcac ttttgcaccg gggaacatcg
     2581 ctcacgactt cccgggtcat cgaccggaaa tcatccaggc acacaccact gcttgcccag
     2641 agataaacct catgccccgc tcgcgtctgt tctttcacca acgcctgaag atatgtggta
     2701 atgccaccgg tattgagatg agttgtaatg tgtaaaatct tcattgttga cgcgacgagc
     2761 aaccgtcacc tgaaataaaa aaaggacctc ccgtgaggaa gatccttttt tgaacgcggt
     2821 taaaattcga gatcaaatct gggaagtgca attcgggcaa cgggtggcct tgagcgatac
     2881 cgtcgaaaaa caaaacgggc actctttcgt cgttgggacc gcgggtgccg gttccggctt
     2941 gcgcttcagg gctgaaattt gtctaatgac aacaaaaata acaaacgcga caatcaaaaa
     3001 gttgatcacc gtattgagga aaaccccgta attcaacgtg ggagccccgg cctgttgcgc
     3061 ctccgtcaaa gaagcataaa cctggccgtt caagctcaga aacaagctcg aaaaatccac
     3121 ccccccaagc aaaagcccaa taggcggcat aatcacatca ttgacgagag aggaaacaat
     3181 cttgccaaac gcgccgccca caatcacacc gaccgccatg tccaccacat tcccacgcat
     3241 gatgaactgt ttaaaatcat ccacgaactt cacgataacc tcctgctgtt ggggggaaaa
     3301 tcacaccggg ttaagttgca ccttcgtgac atcccctccc ttttgtttca gggctgtcac
     3361 ataaagcaaa tagcgaacgt acagggcttc cgccttgcgt ccaacctttt taatatcatt
     3421 ctggagagcc tggttagaga aaaatttttc gatcggaact ttacggatct tttcctgaat
     3481 cttctgaatc tgtgcccgct gggactggat tgccgacaca taagccatcg cgtaatcctc
     3541 aagttcggca acgctcattg tttcaatgtc tttcttgatt ttatcaatcg accgttcgga
     3601 atccactttt ctggtacaac cgttcataac ggtcagcaat cctagtacga ctaataaaat
     3661 ctggagagtt tttcttctca tgcaaaaaga atccttttta ttttgggcta tccaacgtgg
     3721 aggatcacgt cgctcccaag tccttctttt tcgccaaggc ccgagtcaaa aaatcgtcaa
     3781 ttccgttaaa ctttaatccg atttcatacc gttgcccctt agaatctctt ttctgacagc
     3841 ggataacact ggccgtacaa gtttcgggat tggtcaaaaa agggaactgt atctgaagct
     3901 ccagcacact tcccttgggg atcgcctctt tcacgtagca acggattccg acctcactga
     3961 tatctttcac ggtacacacc acaaaggcat gttgagcgtc taaaatatta cggtattttg
     4021 ccagatggta aaccggaagg cgttcagaga gccgcttttc ttttttccag actttcttca
     4081 gcattgaatc cccctttttt tttcggaaca tcccgaccct ttagaaccaa accggccaga
     4141 tcatccaaaa agcatttcga aatcccgtcc cgtttaattt cgtcgttact gatgaaaaag
     4201 ccaatgtttc ttttgccgaa taaaattcaa aataaaaacc gtggacctga ggaggatcga
     4261 actcctgacc tccgcaatgc gaatgcggcg ctctcc
//
LOCUS       DAOSIO010000147         4233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_18466_length_4233_cov_5.300862, whole genome shotgun sequence.
ACCESSION   DAOSIO010000147 DAOSIO010000000
VERSION     DAOSIO010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18466_length_4233_cov_5.300862"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1460
                     /locus_tag="PK590_05960"
     CDS             <1..1460
                     /locus_tag="PK590_05960"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOG24174.1"
                     /translation="AFLVRDHELRFLFLAGSDTRRETGAFTIDYVFSRSSQDKFLILQ
                     TRIEEKEPRFPSLTHFLPQANWQEREVQDLLGLEPIGHPDPRRLVLHEDWPQGVFPLR
                     KDFECSSKIPRVAGDYVFDRVEGEGIFEIPVGPVHAGIIEPGHFRFSVMGESVIKLEI
                     RHFYTHKGTEKSGEGKSPEHLICLAERISGDNSVAHAVACAQALEVLGGMEVPLRAQM
                     IRVIYLELERLHNHMSDIAGISLDAAFSFGASQLMRLKESLLNLNERLTGTRLLRSAV
                     ILGGVCRDISEDEKGRLIETIKKSEEDFDEIVKILGQVDSLIDRAETTGQIKKEIAET
                     LGAVGPVARASGVDRDLRRDHPYAAYRDFSFKVPVFTGGDVESRMNVKIQEAYESFKM
                     ICDALASMPPGPVRGALKPLARSRAAIGYVESPRGECFHWILTDKHGMISRWKVQSPS
                     FSNWPLIEYAVLENIVPDFPLINKSMNLSYSGNDR"
     gene            1485..2390
                     /gene="nuoB"
                     /locus_tag="PK590_05965"
     CDS             1485..2390
                     /gene="nuoB"
                     /locus_tag="PK590_05965"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HOG24175.1"
                     /translation="MFRILRKNIKTKVVRPTPETFSRELQENSRGVPEIDFSKCTGCG
                     KCVLACPTQALKMTDCPEEDRRSFLLSRGSCIFCGLCESACPHQAIAFSGACNLAAST
                     KAELNKKATFPLSVCRKDAAFVAGAAHPGQEKTGEAPDFSRGAFFDRLTVEESGLKLK
                     NKIQNLFGRSLHIREVDAGSCNGCEVEVSNLSNPIYDVERFGIHFVASPRHADMILVT
                     GPVTRAMELALVKTYEAMPSPRLVVACGTCAISGGIFQDSYSVTGGVDHAVPVDLYIP
                     GCPPHPLAIIQGILTLLDKVPHDKK"
     gene            2641..3834
                     /gene="hemW"
                     /locus_tag="PK590_05970"
     CDS             2641..3834
                     /gene="hemW"
                     /locus_tag="PK590_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOG24176.1"
                     /translation="MDKGLYIHIPFCGSKCHYCNFISVPNPDPEIRSRFFRCLFSEIN
                     RAQRQYGTLEFDTLYLGGGTPSLLTTDEMAGLLEKIREHFKFRPDAEVTCEWNPGDRR
                     LPGGEDDPARAKTFSSLGINRISLGAQTFDDSLLARLGRRHTSRDTILTIERIRNAGL
                     SNISLDLMLRIPGQTAEDFQKSIEKAVELGVSQISLYDLEIHAGTVFGRLDRDKKLDL
                     PDERVHAAMYELACHRLPNAGYQHYEISNFAKPPSERHPEGFFSRHNLVYWRNGEYLG
                     LGPGAFSYLSGVRRQFARDLERYLRKCESEDWTNDVEDVLTNEEKEIESLAMRLRLAE
                     GVNPADFPLANSRVSRNLEELLQEGFLKREGMKIRLTPRGQFLSEDVFGFLLRKDDAG
                     NCQAL"
     gene            complement(3884..>4233)
                     /locus_tag="PK590_05975"
     CDS             complement(3884..>4233)
                     /locus_tag="PK590_05975"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012422408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOG24177.1"
                     /translation="LDARYKHLKKEELPLTECLKDTVARVIPLWNDVIAPTIKSGKKV
                     LVAAHGNSLRAMVKFLDNIPENEIVELNIPTGMPLVYELDQNLKPIKHYYLGDPEKVK
                     AAMEAVASQGKAK"
BASE COUNT          948 a         1030 c         1214 g         1041 t
ORIGIN      
        1 tggcgtttct cgtgagggat cacgagctgc gctttttatt tttggccggt tccgatacga
       61 ggcgtgagac cggagccttt acgattgatt acgtcttttc gcgcagttct caagacaagt
      121 ttttgatttt gcaaaccagg attgaagaga aagagccgag gttcccttcg ctgacccatt
      181 ttttaccgca agccaactgg caggaaaggg aagtccagga tctcctgggg ctggagccga
      241 tcgggcatcc ggacccccga cgcctagtcc ttcacgaaga ttggccccag ggcgtgttcc
      301 cgctgaggaa ggattttgaa tgttcttcaa aaataccgag agtggcgggg gattatgttt
      361 ttgaccgggt cgagggcgag ggaatttttg agatccccgt tggtcctgtt catgccggta
      421 ttatcgagcc ggggcatttc cgtttttcag tgatggggga aagcgttatt aaactggaga
      481 tccgtcattt ttacacgcat aagggaaccg aaaaatccgg agaggggaaa agtccggagc
      541 atcttatctg tctcgccgaa agaatttcgg gggataattc ggtggcgcac gcggtggctt
      601 gcgcgcaggc ccttgaggtt ttgggaggca tggaagttcc gttgagggcc cagatgattc
      661 gcgtgatata tcttgaactg gagcgtttgc acaatcacat gagcgacatc gcgggaattt
      721 cactggatgc cgccttcagc tttggcgcca gccagctgat gaggcttaaa gaatctcttc
      781 tgaatttgaa tgaacggttg acgggaacac ggcttttgcg gtcggcggtt attttgggag
      841 gggtttgccg ggacatttct gaagacgaaa agggccggct gatcgagact attaaaaaaa
      901 gcgaagagga ttttgacgaa atcgtgaaaa ttctcggcca ggtggattcc ctgatcgacc
      961 gggccgaaac gaccggccag ataaaaaaag aaatcgcgga aacgcttggc gccgtcggtc
     1021 ctgtcgcgag ggcttccggg gtggatcggg atttgcgacg ggatcatcct tacgcggcgt
     1081 acagggattt ttcgttcaag gtgccggttt ttacgggggg agatgttgag agtcggatga
     1141 acgtgaaaat ccaggaggct tacgaatcat tcaaaatgat ttgtgacgcc cttgcttcaa
     1201 tgcccccggg gcccgtgcgc ggcgcattaa aaccgctcgc gcgatcccgg gccgcgatcg
     1261 ggtatgtcga atctccgcga ggggaatgtt tccactggat cttgaccgac aaacacggaa
     1321 tgatttcccg atggaaggtc cagtccccgt cgttttcgaa ctggcccctg atcgaatacg
     1381 ctgttctgga aaatatcgtg ccggatttcc cgctgatcaa caagagtatg aacctgtctt
     1441 attccgggaa tgaccggtga atatcagatt ttgagaggaa atttatgttt aggatcctga
     1501 ggaaaaatat caaaacgaaa gtggttcggc cgaccccgga aaccttttcc cgcgagctcc
     1561 aggaaaattc tcggggggtg cccgaaattg atttcagtaa atgcacggga tgcggaaaat
     1621 gcgttctagc gtgtccgacc caagccttaa aaatgacgga ttgtccggaa gaagatcggc
     1681 ggtctttcct tttgtcccgc gggagctgta ttttttgcgg tctctgtgaa tcggcctgtc
     1741 cccaccaagc gatcgcgttt tcgggagcgt gtaatcttgc cgcttccact aaagcggagt
     1801 taaacaaaaa ggcgaccttt cctttgtctg tttgccgcaa agacgcggct tttgtggctg
     1861 gtgcggctca cccgggtcag gaaaaaacgg gtgaggcccc tgatttttcc cggggcgctt
     1921 tttttgaccg gcttaccgtg gaagagagcg gattaaaatt gaaaaataaa attcaaaacc
     1981 ttttcggccg gtcccttcat atccgggaag tggacgcggg atcctgcaac ggctgtgagg
     2041 tcgaggtctc gaatctttcc aacccgattt atgatgttga gcgttttgga atccatttcg
     2101 tcgcttcccc gaggcacgcg gacatgattc ttgtgaccgg gcccgtgacg cgggcgatgg
     2161 aactcgcgct cgtgaagact tatgaagcga tgccgtcgcc aaggctagtc gtggcctgcg
     2221 gaacctgcgc catttctggt ggaatttttc aggatagcta ctcggtgacg ggaggcgtgg
     2281 atcacgcggt tcccgtggat ctttacattc cgggttgccc gcctcatccc cttgccatta
     2341 tccaggggat tttgacattg ctggacaagg ttcctcacga caagaaatga agtgacggat
     2401 cgcgggagaa acaatccttt ttacaagcgt tcttgagcgg tttgtccctg ccagtcttgc
     2461 tgaaccgatg gccctgttct tttcttcctc ttgcggcccc gttcttcagc gcgcccatca
     2521 ttaaaaaaat tacgtcgttt gtggaaaggt gcggcgctgg caaagtgaag ggaccatcat
     2581 cacgcgaagc ggaagcgtcc gtgccttggt gaccggaatc tgacgagggt atcattcatt
     2641 atggacaaag gcttgtatat tcacatcccg ttttgtggat cgaaatgcca ttattgcaat
     2701 tttatttccg tgccaaatcc tgacccggaa atccgaagtc gatttttccg gtgtctcttt
     2761 tccgaaataa accgggcgca acggcaatat gggacactag aatttgacac gctttatctg
     2821 gggggaggaa cgccgtctct tttgacgacc gatgaaatgg ccgggcttct tgaaaaaatt
     2881 cgcgaacact tcaagttccg gccggacgcg gaagtgactt gcgagtggaa cccgggcgat
     2941 cggcgcttgc ctggcgggga ggacgacccg gcgcgcgcca aaacgttttc ctcgttggga
     3001 attaaccgga taagcctggg ggcccagacg ttcgacgact cgttactcgc gagattgggt
     3061 cgccggcata cgtcgcgcga tacaatcctc acgatcgaaa gaattcgaaa cgcgggcctc
     3121 tcgaatatca gtctggacct catgctccgg atccccggtc agacggcgga ggattttcaa
     3181 aagtcgatcg agaaagcggt ggagcttgga gtttcccaga tttccctgta cgatctggag
     3241 atccacgcgg gaacggtttt cggacggctt gaccgtgaca aaaagctgga tcttccggat
     3301 gagcgtgttc acgccgcgat gtatgaatta gcctgccacc ggttgccgaa cgcggggtat
     3361 cagcattatg agatctcgaa tttcgcgaaa ccaccgagcg aacgccatcc ggaggggttt
     3421 ttctccaggc acaatcttgt ctactggcga aacggagagt atctcggact gggccccggc
     3481 gccttttctt acctgagcgg agttcgccga caattcgccc gtgatctcga acgctatctc
     3541 agaaaatgcg aatcggaaga ctggaccaac gatgttgaag acgtcctcac gaacgaagaa
     3601 aaagagatcg aaagcctggc gatgaggctt cgtttggccg agggagtcaa tcccgcggat
     3661 tttccgctgg caaactcgcg ggtgtcacga aacctggaag aacttcttca ggaaggattt
     3721 ctgaaacggg aggggatgaa aatccggctg actccccgcg gccaattttt atccgaggac
     3781 gtgttcggat ttttactccg caaagacgac gccgggaatt gtcaggcgct ctgagataaa
     3841 aaaaagaaac ctcgcaacaa aaataatttt cccgtcttcc cgattacttg gcttttccct
     3901 ggctcgcgac cgcttccatg gcggccttca ctttttcagg atcgccgagg tagtaatgct
     3961 tgatcggttt caggttttgg tccagttcgt aaacaagcgg catgccggtc gggatgttca
     4021 gttccacgat ttcgttttcc ggaatgttat cgagaaactt gaccatcgcg cgaaggctgt
     4081 tgccgtgtgc cgcgacaagg actttttttc cggacttgat cgtcggggcg atcacgtcgt
     4141 tccagagggg aattacgcgg gccacggtgt ccttcagaca ttccgtcagc ggcagttctt
     4201 ctttctttaa atgtttgtaa cgggcatcca ggg
//
LOCUS       DAOSIO010000148         4194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_18690_length_4194_cov_4.237497, whole genome shotgun sequence.
ACCESSION   DAOSIO010000148 DAOSIO010000000
VERSION     DAOSIO010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18690_length_4194_cov_4.237497"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(354..>4194)
                     /locus_tag="PK590_05980"
     CDS             complement(354..>4194)
                     /locus_tag="PK590_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOG24178.1"
                     /translation="DSLEAIRNNAELIESDRFINELNKLLGPKYFNHSFSVSPQGTDA
                     VLARALAYYIQKRLTEAGRSDSSAWLATSTEEEERKGIFTPPQDMFNRTLEGVAQTML
                     QEEYFGGDRRGKGIRIVNEFAYKVLSETDPKKIDAIINPLLRQQAQTIADHYFSYVLK
                     AINRENKVRRSEMREAPDLLEVVDEHDQPTGKTIERKQAHQTGERHRAAILFAFNKKG
                     ELLMRKRGAQKDIFPDTWTFSASGHVDAGSTYAKTILKEAQEEMGMPLDPQRLNVVGA
                     SGMPWSDLKQYAYVFKTQDTEAALEATDRARERLIHESVLPIREDSEGLLWRAWPDSS
                     QGTVTVFLYKLSAQRDTLLDDAKDKVGRILREMVGEYEERVITNRDNRTHYVYRLSEQ
                     EEEFLSRRAEDVRRASQTDQAAEVTDLKFFSLDDLRQQFRDHPTLFVDDMLIFFDPQM
                     NRDLEVMEDLSREAKVYEKTSETGLDQNVRELQKRLEEALRDPSADFDAIVFSVNPMF
                     VSVVEETLEGLRGKVFHPKKEIVVLGQDPEGSITDSLSTADRALRKLYGDDYQTQVAD
                     KRVAVITMSGFGARMPTDFGKGLLSLPAGRTYLDQVLRQRFFFLDMDFRGLIVFSIDG
                     INIPQYMPKMGKAGIQVIGMPMARTDENVGELGLFVIDEPFRPGAQPIRYAFEKLSRS
                     QRDDYVARQLITKKYVNVSEANYPISWEAFSWLWEQTQSLRKVLDEKYKQEGKTYRDD
                     NLAYNWAEHLFELAFLPDDLIPDYLERLVARSGSIDKDVFEGYLRIAREARQRFGMEF
                     VNVGAESIYYNTNRPDQYRDFLISLISNQVFWEMFNLRNKDGKIVDQKADVSPAVFSK
                     LVDNGVLVSRSIVDFAVQMKKGSLVFNSALAGGTKIETQGAVINSVGKFVVPRGAIVD
                     TVIAPAEGLEVAPDSYTRGYLVRQTDGTVGQVLVSLPVKILASDGRTAELTVTDVKSS
                     LMGKRLSRYKIFPREEHLTNRTPTLVEESPTKYSLDEVRNNIAHNVSWKLRNALAEAI
                     SENKKSAFQISETMREMVKNEKALVEKRSEVRGVEEEQVELPLADVGIDAAKAMLDAL
                     NAVATQRQEIRLGTGVKSSAAVSVESRLTNLILAIEKQTGKPFPAAFSPDTGAYVTGY
                     NKDRIQNVLAMKEDGLVAEGVVVVIMAASEDDVQAAHNAFGSEIRDRQIEVVRSGANL
                     VAERLSRRYGDSRVQIKPGAVVTEDEAAYLAAAQLANGRVVFVGTNETVVGAYQISVA
                     VMLQSAMEAAQAIATSA"
BASE COUNT         1072 a         1203 c          832 g         1087 t
ORIGIN      
        1 ttcggccctt ccgatccagt ccatgatttt agccgcattg gccgcatagt ccccgacaac
       61 gggattcatc tggccaatcg ctatcttaag gggagaattt ctcatgcgga ggagtatatc
      121 acaacttgac cctgaaaata gttccaggaa cttgaaatcc ggcctgaaac aaatccagca
      181 agaaaaagag aggcacggaa ataaactttt tacaaatccc cttatcggaa atttgtttcc
      241 acttaaaggc gagggggatt ttaaaaatct ccccttcccc tctttgaaaa agaggggaaa
      301 gaccaactac cttcttgttg ctcattaccg cggcatggag ataacgccac ccattacgcc
      361 gatgtggcga tcgcttgcgc ggcttccata gcagactgga gcattacagc cacgctgatc
      421 tggtaggcgc ccacgaccgt ttcattcgtt cctacgaaca ccactctccc gttcgcaagt
      481 tgagccgccg cgagatatgc cgcctcatct tcggtaacaa ctgcgccggg cttgatctga
      541 accctgctat cgccgtaccg tcggctcaaa cgttccgcaa cgagattggc accgctcctt
      601 accacttcaa tctggcgatc tcttatttcg cttccaaacg cattatgggc agcctgaaca
      661 tcgtcttctg aagccgccat aatcaccaca accacaccct ccgcgaccaa tccgtcttct
      721 ttcatagcca acacattctg aatgcgatct ttgttataac ccgtcacata agcgccagta
      781 tccggactga acgccgcggg gaacggcttt cccgtttgtt tctctatggc aagaatcaga
      841 ttcgttaacc gcgactccac agaaaccgcg gcagagcttt tcactcccgt ccccaatctt
      901 atttcctgtc tttgggtcgc caccgcatta agagcgtcga gcatcgcttt cgccgcgtct
      961 atccctacgt cggcgagagg aagctccact tgctcttcct ccacaccacg aacttcagac
     1021 cgtttttcca caagtgcttt ttcattcttc accatttccc tcatagtttc actgatctga
     1081 aaagccgact tcttgttttc tgaaatcgcc tctgctaaag cattacgcaa tttccaagaa
     1141 acattgtgcg caatattgtt ccggacttca tctaacgagt attttgtcgg actttcttcc
     1201 accagagtcg gcgttcgatt ggtcaaatgc tcctccctgg ggaaaatctt gtaacgactc
     1261 aatcgtttcc ccatcaagct actcttaacg tcagtcaccg taagctccgc ggttctacca
     1321 tcggatgcaa gaattttgac tggcaaggac accagcactt gccccacagt cccgtccgtt
     1381 tgacggacaa gataacctcg ggtatagctg tcgggtgcca cttcgagacc ttccgcggga
     1441 gcaataaccg tatcaacaat cgcgccgcgt ggaaccacaa actttccgac actgttaatg
     1501 actgcgcctt gagtttctat cttggtacca cccgctaacg cactattaaa aaccaaactg
     1561 ccttttttca tttgaacggc aaaatcaacg atgctccggg aaaccagaac accgttatca
     1621 accagcttgc taaaaacggc ggggctcaca tccgcctttt gatctacaat tttcccatct
     1681 ttatttcgca gattaaacat ctcccaaaaa acctgattcg atatcaatga aatcagaaaa
     1741 tcacgatatt ggtcggggcg gttcgtattg tagtaaattg actcagcccc cacattgacg
     1801 aactccatcc caaaccgttg cctcgcctca cgggcaatcc tcaaataacc ttcgaacaca
     1861 tccttatcaa tagaacccga cctcgccacc aggcgttcaa ggtaatccgg aatcaaatca
     1921 tcaggaagaa acgccaactc aaacaaatgc tcagcccagt tgtaggccag attatcatcc
     1981 cgataagttt tgccttcctg cttatatttc tcatccagaa ctttccggag agactgcgtt
     2041 tgttcccaaa gccaactaaa ggcttcccaa gaaattggat aattcgcttc cgaaacgtta
     2101 acatattttt ttgtaatcag ttgccgcgcc acataatcgt cgcgttgaga acgagaaagc
     2161 ttttcaaagg cataacggat cggctgtgct ccgggtcgga aaggctcgtc tattacaaaa
     2221 agaccgagtt cccccacatt ttcatccgtc ctcgccatcg gcattccaat cacctggata
     2281 cccgccttac ccattttagg catgtattga ggaatattga tcccatctat cgaaaaaacg
     2341 atcaaccccc ggaaatccat atctaaaaag aaaaacctct gacgcaacac ttgatccaga
     2401 taggttcgtc ccgctggcaa cgacaacaat ccctttccaa aatctgttgg catcctggct
     2461 ccgaaaccgg acatggtgat aacagcaacg cgtttgtctg ctacttgtgt ctgataatcg
     2521 tcaccataca acttacgtag tgccctgtcg gctgtactaa gagaatccgt aatacttcct
     2581 tcaggatcct ggcccagcac aacgatctcc ttcttcggat gaaatacctt tcctcttaat
     2641 ccttccaaag tttcctcaac aacagacaca aacatcggat tcacggaaaa aacaatcgca
     2701 tcaaaatcag ccgaaggatc cctcagagct tcttctaagc gcttctgcaa ttcccggaca
     2761 ttttgatcaa ggcctgtctc agaagttttt tcataaacct tggcctctcg agatagatct
     2821 tccattacct ccaaatcccg gttcatttgg ggatcaaaaa agatcaacat atcgtcaaca
     2881 aacagagtcg gatgatcacg aaactgttgg cgaagatcat ccagtgaaaa aaacttaaga
     2941 tctgtcactt ccgcagcctg atctgtctgt gaggcacgac gcacatcttc agcccgccgg
     3001 gacaaaaact cctcttcttg ctcgctcaag cgatacacgt aatgggtccg gttatcacgg
     3061 ttcgtaatca cgcgctcttc gtactcccca accatttccc gcaaaattcg ccctactttg
     3121 tctttggcat cgtctaacaa ggtatctcgt tgagcgctaa gcttgtagag gaaaactgtc
     3181 acagttcctt ggctcgaatc gggccaagcc ctccagagta atccttctga atcctccctt
     3241 atgggaagga cactctcatg aataagacgc tcacgcgcac gatctgtcgc ttccaaagcc
     3301 gcctcggtat cttgagtctt aaaaacatac gcatattgtt tgaggtctga ccacggcatc
     3361 ccgctggcac ccacaacatt caacctctgc ggatcaagag gcattcccat ttcttcctgt
     3421 gcttctttca gaatggtctt tgcatacgtc gaaccagcgt ctacgtgccc actcgcagaa
     3481 aacgtccaag tatccgggaa aatatctttt tgggcgccac gctttcgcat gagcagttca
     3541 ccctttttgt tgaacgcgaa aagaatcgcc gcacgatgac gttctccggt ttgatgggct
     3601 tgcttgcgct caatcgtttt acccgtcggc tgatcatgtt catcaacgac ctccaggaga
     3661 tcaggagcct ctcgcatttc tgaacgccgc accttatttt cccgattgat cgccttcagg
     3721 acataactga agtagtgatc cgcaatcgtt tgagcttgct ggcgcagtaa cggattaatg
     3781 atcgcgtcga ttttctttgg atcggtttca gaaagcacct tgtaagcgaa ctcgtttacg
     3841 atgcggattc ctttccctct ccgatctccc ccaaaatatt cttcctgaag catcgtttgc
     3901 gcgaccccct ccaaggtgcg attaaacata tcctgtggcg gcgtgaaaat tcccttccgc
     3961 tcttcttctt cagtcgacgt cgcaagccac gcggaactgt cgctacggcc agcttcagtc
     4021 aaacgttttt gaatgtaata ggcaagggca cgcgccaaga cagcatcggt accctgtggg
     4081 gatacactaa aagaatgatt gaaatatttc ggtccgagca acttgttgag ttcattgata
     4141 aaacggtcgc tttcgatcaa ttcggcgtta tttcgaatcg cttccaagga atcc
//
LOCUS       DAOSIO010000149         4191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_18705_length_4191_cov_3.321809, whole genome shotgun sequence.
ACCESSION   DAOSIO010000149 DAOSIO010000000
VERSION     DAOSIO010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18705_length_4191_cov_3.321809"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1215)
                     /gene="rlmN"
                     /locus_tag="PK590_05985"
     CDS             complement(103..1215)
                     /gene="rlmN"
                     /locus_tag="PK590_05985"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOG24179.1"
                     /translation="MNPAKERPLSRRSLYGLTPPEIESLVVASGFPKFRASQILDWLY
                     KKNVLSWNEMKNLPKEVRHALASNYSLTVLERCEPGETQGEGTCKYLFRTLDGHFLES
                     VLISSRGESVGQRERETVCVSTQLGCPVKCAFCASGKGDFVRNLAAGEIIEQIVQISA
                     KSDRQITNVVFMGMGEPLENFEEVMKALNILTAQWGFEMGGRKITLSTCGVTDRIYEF
                     VRRTEGRIRLSVSLHSPEDESRSRLVPYNRKYPLRELLAELEKVHRTLKRDITFEYTL
                     IEGVNDSAEDAKKVAQLAKPLRAKINLIPYNPIREIVWKTPSEKQMNLFRETLEKAGV
                     RSTFRKTAGREVDGACGQLRLDRSIRRQATRHKDIR"
     gene            complement(1238..2215)
                     /gene="corA"
                     /locus_tag="PK590_05990"
     CDS             complement(1238..2215)
                     /gene="corA"
                     /locus_tag="PK590_05990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOG24180.1"
                     /translation="MISSFLYKKGAAVESNLSRAGMLAALQEKGCLLWVDFESPSEFE
                     EETLVEIFNFHPLAVEDCIADQSQPKIDDYEEYLFLVVHAITMCEKGKQKELSTIGLN
                     IFIGDNYVVTFHKDPIPGIAQVREVIQKKAQVLMGGTSDLLVHSILDHLVDGYMPVLH
                     EYDDKIDALEQHMFENGSRDYLNTLLQIKQDIFTLRRVIAPQRDTLYSLTRNPTRLIK
                     TKNLMYFRDVYDHIFRIQSMAESYQDTLNSILQVYFSYTSTKLNEVTKRLTVLATITM
                     PPVIVASIYGMNFRHMPELEWPFGYAFALGLCIVSSLGMFLWMKFKKWI"
     gene            2332..2703
                     /locus_tag="PK590_05995"
     CDS             2332..2703
                     /locus_tag="PK590_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG24181.1"
                     /translation="MAKKVVVIDDEPEILKMVSSSLLEAGYATYGAPNGPDGLALVKK
                     EIPDLVILDLGLPGLNGLEVLKELRRTCPQTLVIVLSADREPETLKKALAAGASEYIT
                     KPINLETLRDQFVKDLIGPSS"
     gene            complement(2714..3079)
                     /locus_tag="PK590_06000"
     CDS             complement(2714..3079)
                     /locus_tag="PK590_06000"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOG24182.1"
                     /translation="MTKREVINRSNRRKHVRVDVYAVTRYFCAVRDKEVGVQTRISDI
                     SEGGAKLLTFEEGIPVDTVVSMSFRLPHQENVVIKVEGMVRYSGFVEQDLFRTGVEFT
                     KARKKDIEVIREYVYSRTR"
     gene            complement(3257..3346)
                     /locus_tag="PK590_06005"
     tRNA            complement(3257..3346)
                     /locus_tag="PK590_06005"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3310..3312),aa:Ser,seq:tga)
     gene            complement(3466..3993)
                     /locus_tag="PK590_06010"
     CDS             complement(3466..3993)
                     /locus_tag="PK590_06010"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOG24183.1"
                     /translation="MEKPDHQHFMKEAIKQAVTALEKGEVPVGAVVVWKNKIVARAYN
                     QMEMLRDPTAHAEMIALTQASETLAGMEEAKAEIPVKRNNQTPDHRGSLEGATLYVTL
                     EPCPMCAGALVMTKCENLVYGARDPKAGACHSLYHITDDERLNHRVKVTGGVMAEDAK
                     FLLGEFFKSLRKDRR"
BASE COUNT         1045 a         1108 c          932 g         1106 t
ORIGIN      
        1 aactaacgag aatcggcgcg aaaatctatg tttattaaaa gattctgcgt gaaaccttct
       61 ccggaagact ttaaaaagca tcgcggacta acctttttgc ctttatcgta tgtctttgtg
      121 gcgcgtggct tggcggcgta tcgagcgatc cagcctgagt tgcccacatg cgccatcaac
      181 ctcacgacct gccgttttac gaaaggtcga acgaaccccc gctttttcga gggtctcgcg
      241 aaaaagattc atttgttttt cggacggagt cttccacacg atttcccgga tcggattgta
      301 gggaatcaga tttattttgg cccgaagggg cttggcgagt tgagcgacct ttttggcatc
      361 ttctgccgag tcattcactc cctcgataag ggtatattca aatgtgatgt cgcgtttaag
      421 agttcgatga actttctcaa gttccgcgag gagctcccga aggggatatt tcctgttgta
      481 aggaacgagc cggctacgcg attcgtcttc cggggaatgc agagaaaccg aaaggcgaat
      541 tcttccttcc gtgcgtctta cgaattcata aatccggtcc gtcactccgc aagtagaaag
      601 ggtgatcttt cgccccccca tttcaaagcc ccattgagcg gtgagaatat tcagcgcttt
      661 catcacttct tcaaaatttt caagcggctc gcccataccc ataaaaacaa cgttcgtgat
      721 ctgtcggtcg ctttttgccg agatctgaac aatttgttct atgatctctc cggccgcaag
      781 attacggaca aaatcacctt tgccacttgc gcaaaacgcg catttaacag gacatcccag
      841 ctgtgttgaa acgcaaaccg tttcccgctc ccgctgacca accgactccc cccgggagga
      901 aataaggacg ctctccagaa aatgtccgtc caaagtacgg aacaggtatt tgcatgtccc
      961 ttcaccttga gtttctcccg gttcgcacct ttcaaggacg gtgagagaat agtttgaagc
     1021 aagggcgtga cgaacttctt tcggaagatt tttcatctcg ttccaggaaa ggacgttttt
     1081 tttgtaaagc cagtcgagga tttgagaagc tcgaaatttg gggaatccgg acgccacgac
     1141 aagggattcg atttcaggcg gggtcaaacc ataaagcgat cgacgggaaa gaggtcgttc
     1201 tttcgccgga ttcatgtctc cgtgccccgt atcgctttca gatccacttc ttaaatttca
     1261 tccagagaaa catccccaga gaggagacga tacagagccc gagagcaaac gcatagccaa
     1321 acggccattc gagttcgggc atgtgccgga aattcatgcc gtagatactt gccacaatca
     1381 ccggcggcat cgtgatcgtc gcgagcactg tcaatcgctt ggtcacttca ttcagcttgg
     1441 tcgacgtata ggaaaaatag acctggagaa tgctgtttaa cgtatcctga taactttccg
     1501 ccatgctttg aatcctgaaa atgtgatcat atacgtcacg aaaatacata aggtttttcg
     1561 ttttgattaa tcgggtcgga tttcgagtca gagaataaag cgtatcgcgc tggggagcga
     1621 tcacccggcg caacgtaaag atatcctgct tgatctggag aagcgtgttt aaataatccc
     1681 tggacccgtt ttcaaacata tgctgttcaa gggcgtcaat cttgtcgtca tattcgtgca
     1741 ggaccggcat atatccatcc accaaatgat ccaggattga gtggaccaga aggtcagaag
     1801 tccctcccat cagcacttgc gccttctttt gaataacctc tcgcacttgc gcgatgccag
     1861 ggatcgggtc cttgtggaag gtaacaacgt aattgtcccc gataaaaata ttcaacccga
     1921 tggtggagag ctccttttgt ttgccttttt cgcacatcgt gatcgcatga accacgagaa
     1981 aaagatattc ctcgtaatcg tcaatcttgg gctgagattg gtccgcgata cagtcctcaa
     2041 ccgccagagg gtgaaaattg aaaatctcga ctagggtttc ttcttcaaat tcactgggac
     2101 tttcaaaatc cacccaaaga agacagccct tttcctggag cgcggccaac atgccggcgc
     2161 gcgaaagatt tgactcgacg gcggcgcctt ttttatacag gaaacttgaa atcataaagg
     2221 ttctccattt ttttcctagg gtatgatagc aatcgttcta ccggtttacg acataaaatt
     2281 tcttttcggt aagatcttgt tttataataa atgccatcca gtggaggcgc catggcaaaa
     2341 aaagtagtcg tcatcgacga tgaacctgaa atcctgaaaa tggtttcaag ctcccttctc
     2401 gaagcgggat atgccaccta cggcgctccc aacggtccgg acggtcttgc cctcgtcaaa
     2461 aaagagatcc ccgacctcgt catcctggat ctcggccttc ccggattaaa cggccttgaa
     2521 gtcctgaaag aactgcgccg gacctgcccg caaactctgg tgatcgtctt aagcgcggat
     2581 cgggaaccgg agacgctgaa aaaagccttg gcggccgggg catcggaata catcaccaaa
     2641 cccatcaacc ttgaaactct tcgcgatcag ttcgtcaaag acctgatcgg cccatcgtct
     2701 tgattattcc gctctagcgg gttcggctgt aaacatactc acggataact tcaatgtctt
     2761 ttttgcgcgc tttggtaaat tcaacaccag ttcggaaaag atcctgttcc acaaatccac
     2821 tatagcgaac catgccttca actttaataa caacattttc ctggtggggt agtcgaaagc
     2881 tcatcgacac taccgtatcg acgggaattc cctcttcaaa ggtcaacagt ttcgccccgc
     2941 cttccgaaat atcagaaata cgcgtttgta cgcctacttc cttgtcccga acagcgcaaa
     3001 aataccgggt caccgcgtaa acatcgaccc ggacatgttt cctgcgattg cttcgattta
     3061 ttacttcacg ctttgtcatt ttttatcttt cttagctttg ttaatgaacg caacatttgc
     3121 ataatatttt gccataaaac atgacaaata aataagcctc accacgccta aattcaaagt
     3181 ccgtcttcgt tcttgtctaa acagtcgagc agatcctcgg agagaaacag acccgccaga
     3241 tttgataacg caaatctggc ggagggagta ggattcgaac ctacgagacc cttgcgggcc
     3301 taacggtttt caagaccgcc gcattcaacc gctctgccat ccctccgttg gtaaattcat
     3361 ttccggctac ttgttccaaa gaacaagctt accgctcgca ttctccgtca cgaaacattg
     3421 gtcacaaaag gctttctgcg taccatggag ccctgtgacg cgcggttatc ttcggtcttt
     3481 cctcaggctt ttaaaaaatt ctcccaacaa aaacttggca tcctccgcca taacgccacc
     3541 ggtcaccttc acacggtggt tcaagcgctc atcgtccgta atatgataaa ggctgtggca
     3601 agctccggcc ttggggtccc gggcgccata aacaagattt tcacatttag tcatcacaag
     3661 cgcgccggcg cacattggac acggctcaag cgtaacatac agggtcgctc cttccagaga
     3721 cccgcgatgg tcaggcgtct gattgttccg ctttacggga atttccgctt tagcttcctc
     3781 cataccggca agggtctcgg aagcctgggt caacgctatc atttccgcgt gagccgttgg
     3841 atcacgtagc atttccatct gattgtacgc tcgcgctaca attttattct tccacaccac
     3901 gaccgcgccg accggaacct ctcccttttc cagagcggtc accgcctgtt tgatcgcttc
     3961 tttcatgaaa tgctggtgat cgggcttttc cacttcgttt tgtctccaat ttgccgtttg
     4021 aacaagcggt attatacata aactgaaaat ggagacagta actttaacaa aaaactggcc
     4081 gtctccgctt cttttctgtt atcctctcgg ccatgtcatt aacaaactct ccgaaagtta
     4141 gtccatccca atggcttcct ctggcagttg ttttcgccgt cgtgtatttc t
//
LOCUS       DAOSIO010000150         4152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_18941_length_4152_cov_3.600439, whole genome shotgun sequence.
ACCESSION   DAOSIO010000150 DAOSIO010000000
VERSION     DAOSIO010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18941_length_4152_cov_3.600439"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PK590_06015"
     CDS             complement(<1..327)
                     /locus_tag="PK590_06015"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOG24184.1"
                     /translation="MKSGSIRVCGLGKKGFTLVEVVLSIVVLAVGLVFVQRTLLRSFT
                     ALNVIKHWSQAEQILDEKIWDVKRRVREQGSTVQRESLNETFLGNERVYRGNMDIHWM
                     DPQGRLI"
     gene            complement(329..775)
                     /gene="gspG"
                     /locus_tag="PK590_06020"
     CDS             complement(329..775)
                     /gene="gspG"
                     /locus_tag="PK590_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOG24185.1"
                     /translation="MCKKKQGGFSLIEIILVVVIIATLSAMVVPRLTGRSEQARLAAA
                     RADVEVNIPTALKLYELDNGYLPTTAQGLNALLQKPSTAPIPENWNGPYLEKFPVDPW
                     GRPYQYQSPGTNRPHDYDLFSLGKDADPDSTKDDVVNWIVTGALAK"
     gene            complement(790..2010)
                     /locus_tag="PK590_06025"
     CDS             complement(790..2010)
                     /locus_tag="PK590_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOG24186.1"
                     /translation="MSQFRYEAKRGKDLIKGILSADTKDAAIDKIIEMGLVPVELFEE
                     KGGGKQSRVLGRNQARTLKSSGHTLIVFYRQLSRLLKSGVPLLPALVLVSEQAEEGKY
                     YQVLDAIKNQVRQGQSLAEAMAEYPEIFNAFTLAVVEIGAHTGHLDDALQRLADYYEK
                     QSATRQKVKNALTYPIFVLALGGLALVFLLSYVVPKFSKLFLDLGQALPWLTRVLVGL
                     SEITCQYGFWFLLGAGVLVFFVHNRLKIRAHRLSWDRLKLRLPLVGKIIFMAQFALFA
                     RSMEMLLKGGVPLLKALRTAIPAVPNEAMKEDLLLVERLVEQGASFSDSLKSGGTFPI
                     FAIHLLLVGEQTGRMDQSFEDIADWYEKESSDAVRSFVQLIEPVTIFVIGAVLGIVAI
                     AILLPVFSIDTLVS"
     gene            complement(1997..>4152)
                     /locus_tag="PK590_06030"
     CDS             complement(1997..>4152)
                     /locus_tag="PK590_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOG24187.1"
                     /translation="EHALLDIFSKATGRPVVSLKTLTPDPTVFDHVPVKFASYYHFFP
                     IRVEKGKLLIATSRIFDINTLDEMRFAMGYEIEICFAPEREIQEMLRRHYGLAADTVE
                     KILSETSPQDHPVPVSEVPEVEDIEKLAQTPSVAHVVNQIILDAYKKRASDIHLEPLQ
                     SGIRIRYRIDGILQEAPVPRELKDFFAPMLSRIKIMANLNVAEKRLPQDGKFRVKTQE
                     ETLDLRVSFIPTANGESVVIRILPGKNVFRLEQLGFETKHQKILEELLNHHNGILFVT
                     GPTGSGKSTTLYASLNKLNSPDRKIITIEDPVEYELEGVNQIHVNPDIGLSFANGLRS
                     MLRQDPDVMMVGEVRDLETADIAIRAALTGHLILSTLHTNDAASGVTRLLDIGVAPYL
                     IASSVVAFIAQRLVRVICPVCKAEVPDIKEEVKNLIEREYGIRETDIRIFRGKGCEQC
                     GHTGFRGRLAIHEVLRLTEPIRKLIMDRATAESIKTEAVRQGMTTLRFNGWEKVLLGL
                     TTVEEVLETTEDDRLENWTAESKDTVPEVIRIAEEKSGVGSLPSVEPQADSDNKFTNL
                     RKFQRLSCEVPVFFKIVDYKGEDARIIQAKKELMQVEFEGVSRDLSAGGIYFSVLDQP
                     ITDKIGAIEGPAILLWESLEVGSVLDIRILLPKGEKSVRCMAKICRVFRSMGQASSGV
                     TAWCRIGLSFLALDSDDRHRLMKFSKINAESNEPV"
BASE COUNT         1108 a         1166 c          877 g         1001 t
ORIGIN      
        1 gattaaacgc ccctgaggat ccatccagtg aatatccata ttgccgcggt aaaccctttc
       61 gttcccgaga aacgtctcgt tcagactttc ccgctgaaca gtgcttccct gctcgcgaac
      121 tcgtcgtttc acgtcccaaa ttttctcatc caaaatctgc tcggcctgac tccagtgttt
      181 aatcacattt aaggctgtga atgagcgcaa taaagttctc tgaacaaaaa ccagccccac
      241 cgccaaaacg acaatactca aaaccacctc gacgagcgta aaaccctttt tgcccagacc
      301 acaaacacgt atggatccgg atttcatact acttggcaag cgcccccgta acaatccagt
      361 tcacgacatc gtccttggtc gaatcgggat cagcatcctt tcccagcgaa aaaagatcat
      421 agtcgtgggg ccgattggtt ccgggggatt gatactggta cggacgtccc caaggatcca
      481 ccggaaattt ttcgaggtaa ggtccgttcc agttttcggg aatcggggcc gtcgacggtt
      541 tttgaaggag cgcattaagc ccttgggccg tcgttggtaa atatccgtta tccagctcat
      601 aaagtttcag cgccgtaggg atattaactt cgacatccgc gcgcgcggcg gccagtcgcg
      661 cctgttcaga ccggcctgtc aaacgcggaa ccaccatcgc cgaaagggtc gcgataatca
      721 ccaccaccag aataatctcg atcagggaaa aacctccttg ttttttctta cacatatttc
      781 ctccacaggt tacgaaacaa gagtatcaat actgaaaacc ggtaataaaa tcgcgatcgc
      841 gacaattcca agaaccgccc cgatcacgaa aatcgttacc ggctctatta gctggacaaa
      901 cgagcgaacc gcatcagaag actctttttc gtaccaatcg gcaatatctt cgaaagattg
      961 atccatccgg cccgtttgtt cccccaccag aagtaaatgt atcgcaaaaa tcggaaacgt
     1021 tccgccgctt ttgagcgaat cactgaaact ggcgccctgt tcaaccaacc gttccaccag
     1081 caaaagatcc tccttcatcg cttcatttgg aacggcagga atagccgtgc gaagcgcctt
     1141 cagcaggggc actccacctt tcaacagcat ctccattgaa cgggcaaaaa gcgcgaactg
     1201 ggccataaaa ataattttcc caaccagggg taatcgcaat ttaagccggt cccaagaaag
     1261 ccgatgggcg cggattttta agcgattatg aacaaaaaac accaacacgc cagcacccaa
     1321 cagaaaccag aacccgtatt gacacgttat ttcacttaat ccgacaagta cccgcgtcaa
     1381 ccagggcaag gcctgcccaa gatccagaaa aagctttgag aatttcggaa caacataact
     1441 cagcaaaaaa acaagtgcta aaccgcccaa tgccaaaaca aagatcggat aagtcagcgc
     1501 gttcttcact ttctggcgcg tggccgattg cttttcataa taatccgcca gacgctgtaa
     1561 cgcatcatcc agatgtcccg tgtgcgcgcc aatctcaaca acggccaacg tgaaagcgtt
     1621 aaagatttcg ggatattccg ccatagcctc agccaatgac tgtccctgac gcacctggtt
     1681 tttgatggca tccagcacct gataatattt cccttcctca gcctgctcgg ataccagaac
     1741 caacgcggga agaagtggaa cgccagattt caaaagacgg ctaagttgac gataaaaaac
     1801 aataagcgtg tgcccgctac ttttaagggt ccgggcctga tttctcccca aaacccggct
     1861 ttgttttcct cctcccttct cctcaaacaa ttcaaccgga acaagtccca tctcgataat
     1921 tttatcgatt gccgcgtctt tcgtgtcagc agaaagaatg cccttgataa gatccttacc
     1981 tcttttggct tcgtacctaa actggctcat tggattccgc attgattttc gaaaatttca
     2041 tgagccggtg ccgatcatca ctgtcaaggg caagaaacga taatccgatt cgacaccagg
     2101 ccgtaacacc gctactggcc tgtcccattg aacgaaaaac cctgcaaatc ttggccatgc
     2161 accgcacaga tttttcaccc ttgggtagca aaatcctgat atctagaaca ctccccacct
     2221 cgagtgactc ccaaagcaaa atcgcagggc cttcaatggc tccgattttg tccgtaatcg
     2281 gttgatcaag aactgaaaaa tagattccac cagcgctaag atcgcgagac actccttcaa
     2341 actctacttg cataagctct tttttcgctt gaataattcg ggcatcctcc cccttatagt
     2401 ccacgatctt aaaaaaaaca ggaacctcac aactcaggcg ttgaaacttt cttaaattcg
     2461 taaatttatt atccgaatcc gcctggggtt ccactgaagg caacgaacca actccggatt
     2521 tttcttccgc gatgcgaatc acttcaggga ctgtatcctt tgattcagcc gtccagtttt
     2581 ccaaacgatc atcttccgtc gtttccaaaa cttcttcaac ggtcgtcaga ccaagcaaaa
     2641 ccttctccca gccgttaaaa cgcaaggtcg tcattccctg tcgaacagct tccgtcttaa
     2701 tcgattccgc ggttgcgcga tccatgatca gcttccggat tggctccgtc aagcgaagaa
     2761 cttcgtgtat cgccagtcga ccgcgaaatc ccgtgtggcc acattgctca caccccttcc
     2821 ccctgaaaat ccgaatatcc gtttcccgaa ttccatattc ccgttctatt aaatttttaa
     2881 cttcttcctt gatatccgga acttccgcct tgcaaacagg acagatgacc cgaaccagac
     2941 gttgcgcgat aaacgcgaca acgcttgagg ctattaaata tggcgccaca cctatatcca
     3001 gcaaacgggt cactccactc gccgcgtcat tcgtgtgtaa cgtggaaagg atcaaatgcc
     3061 ccgtcagcgc ggcgcgaatg gcaatgtcgg ccgtctccag atctctcacc tcacccacca
     3121 tcatcacatc aggatcctgg cggagcatac ttctcaatcc gttggcaaaa ctaagtccga
     3181 tatcgggatt aacatggatt tggttaaccc cctccaactc atactccacc ggatcttcga
     3241 tcgtgatgat cttccggtct ggagaattaa gcttgtttaa cgacgcgtag agcgttgtgc
     3301 ttttgccgct tcccgtcggc cctgtcacaa aaaggatccc gttgtgatgg ttgagaagtt
     3361 cctcaagaat tttttggtgt ttcgtctcga aaccgagttg ttcaaggcga aacacatttt
     3421 tccccggaag aattcggatc acgacgctct ccccattcgc ggtgggaata aaagacacgc
     3481 gaaggtccag cgtctcttcc tgggttttga cacgaaattt gccgtcctga ggcagacgtt
     3541 tttcagccac attaagattt gccataatct tgattcgtga aagcatcgga gcgaaaaaat
     3601 ccttgagttc ccgcgggacc ggggcttcct gcaaaattcc gtcaattcga taacggatac
     3661 ggatgccgga ctggaggggc tcaagatgaa tgtcgctcgc acgctttttg taggcatcca
     3721 gaataatctg gttgacgaca tgggccaccg acggggtctg ggccagcttc tcgatatctt
     3781 caacttctgg cacttcagat acgggcacgg gatgatcttg cggggaggtt tcggaaagaa
     3841 ttttttccac cgtatctgca gcaaggccat aatgccgccg gagcatctcc tggatttccc
     3901 tctccggagc aaaacagatt tctatttcat atcccatcgc gaatcgcatt tcatccagcg
     3961 tgttgatatc aaaaatccgg gaagtcgcga tgagaagctt gcctttttcc acccggatcg
     4021 gaaaaaagtg gtaataggac gcaaacttca ccggaacatg atcaaacacc gtaggatccg
     4081 gagtcaaagt tttaaggctg accacaggac gtccggtcgc ttttgaaaaa atatcaagaa
     4141 gcgcatgttc ct
//
LOCUS       DAOSIO010000151         4147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_18973_length_4147_cov_4.778104, whole genome shotgun sequence.
ACCESSION   DAOSIO010000151 DAOSIO010000000
VERSION     DAOSIO010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18973_length_4147_cov_4.778104"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..507)
                     /locus_tag="PK590_06035"
     CDS             complement(67..507)
                     /locus_tag="PK590_06035"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HOG24188.1"
                     /translation="MSGASGLKSRSKLIVYSDGGARGNPGPAAVGVLVCDEQGHKLGD
                     HSEVIGETTNNVAEYTAVLRGLTLAAELKGVEVDYFLDSELVARQLSGIYKLKAEHLR
                     PLFTDVKEKEKKFKRVTYFHVPREHEKIRYVDKLVNIALNKAGC"
     gene            complement(547..1245)
                     /gene="cysE"
                     /locus_tag="PK590_06040"
     CDS             complement(547..1245)
                     /gene="cysE"
                     /locus_tag="PK590_06040"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOG24189.1"
                     /translation="MTNPFYAIYRDFKACFDRDPAARTPLGILEVIFLYSGFHAVLFY
                     RFANVLHKLKIPFIPRFISQLARFLTGVEIHPGAQIAPGFFIDHSMGVVIGETTIIGN
                     NVTIYQGVTLGGTGKERGKRHPTLGHNVVVGVGAKILGNIRIGNHVKVGGGSVVIHSV
                     PDYCTVVGVPGEIVRIDGKKPEEIDLEHGDLPDPVKELKNRLRALQKEVDQIEAQHKG
                     TRSGQQSTSGEKDG"
     gene            complement(1381..2475)
                     /gene="ychF"
                     /locus_tag="PK590_06045"
     CDS             complement(1381..2475)
                     /gene="ychF"
                     /locus_tag="PK590_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOG24190.1"
                     /translation="MGFRCGIVGLPNVGKSTIFNALAKAHAAASNYPFCTIEPNVGIV
                     PVPDPRLTELSNLYHPQKTTPTTVEFYDIAGLVAGASKGEGLGNQFLGHIKEVEAILH
                     VVRCFEDPNIVHVASTVDPVRDIEVIDTELCLKDLDTINARYEKTEKLAKTGDKDAKE
                     RMPVYAKVKTALEAGTPLRRVGFTDEEKKLLKDMAFLTLKTVLYGANVAETDLPSGGP
                     HVDEVRKKAAEENAEVVVISGKVESELIDLSEEDQQAFLKELGLAESGLHSLIRAGYR
                     ALNLVTYFTAGEKEVRAWTVRNGAKAPEAAGVIHSDFERGFIRAEVMRCEDLLQYKTP
                     AAVKEKGLLRIEGKDYQVRDGDTMLFRFSV"
     gene            2704..>4147
                     /locus_tag="PK590_06050"
     CDS             2704..>4147
                     /locus_tag="PK590_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24191.1"
                     /translation="MVSTFQPLAESSWTKPLQPSFAGGELVCLAAHPKDHTRFLVANR
                     SQIFEGASSLDWRMLWSSTGRQANIKKILTFPLLPDQIFVLTPEEILMGDLRSGAWAS
                     FYSSGGNPDKSPLSFTPDPANPNLWFAGTSRGLWTTENSGKTWSRSELVPSEKAVPLL
                     HYAEDRLFIGSENSIYLVQRETSPKHVFELPQKEKDFSEAEEALKDKTAFYHARIFEL
                     IEAPVSSGHFFLGTDDGVFETSDGGLNWFPLPQSGLQSAVILDLVFSEKTNLLFAATP
                     RGVFQYQANKKSWRELSRGLARSNSRSLAIMNESTLAAITGDGFVQYSLFPETPEPTS
                     LFMPGAETLNLFRKLVVLEPSARDIQREAIRYANVSNWKTRRWHVASRAAALIPSLSF
                     GKTMYRNSSLSTYGGKFFAGPEDVNKTFDTDIRWDLGNALFSSSQTSIDSREKLMVDL
                     RRDILSETTRIYYERRRLQIDLIYTPSLSEQ"
BASE COUNT          981 a         1149 c          964 g         1053 t
ORIGIN      
        1 accccgagaa gtcctcccgt gaacccaccc atcaacccaa tccccgccgt cacccaatcc
       61 ataaaatcaa caacccgcct tattcaacgc gatattaacg agtttatcga cgtatcttat
      121 tttttcatgc tcccgcggaa cgtgaaagta ggtcacccgt ttgaattttt tttctttttc
      181 ttttacgtcc gtgaacagcg gacgcagatg ctccgctttc agtttataaa ttccggaaag
      241 ttggcgtgcc acaagttcac tgtccagaaa ataatctact tccacgcctt tcaattccgc
      301 cgcgagtgtg agcccccgaa gaaccgccgt atactcggcg acattatttg tcgtctcgcc
      361 aatcacttcg ctgtgatctc ccaacttatg gccttgctcg tcacatacca gaacgccaac
      421 agcggcgggg cccggattcc ccctggctcc gccgtcagaa taaacgatca gttttgaacg
      481 cgattttaat ccggaagctc cgctcatatc agggaagatc ccaggcgcga cgcatagcgt
      541 aatcggttag ccatcttttt cgccgctggt tgattgttga ccgctccgtg tgcctttgtg
      601 ctgggcctca atctgatcga cttctttctg gagcgcgcga agacggtttt tcagttcctt
      661 caccggatcc ggaagatcgc cgtgctccag atcgatttct tcgggtttct ttccgtcaat
      721 ccggacgatc tctcccggaa cgccaacaac ggtgcagtaa tcaggaacgg aatggattac
      781 gacggagcct ccgccaactt tgacgtggtt tccgattcgg atattaccaa gtatctttgc
      841 tccgacgcca accaccacat tatggccaag ggtcggatgc cgttttccgc gctcttttcc
      901 cgtaccgcca agcgtcaccc cctgatagat agtgacattg ttaccgatga tcgtcgtctc
      961 accgataaca actcccatgc tgtgatcgat aaaaaatccc ggggcaatct gcgcccccgg
     1021 atgaatttca acgcctgtca gaaatctcgc gagctgggaa atgaaccggg ggataaaagg
     1081 gatcttgagt ttgtgaagca cgttcgcgaa ccggtaaaaa agcaccgcgt gaaatcccga
     1141 gtaaagaaag ataacttcca gaattccgag aggtgttcgc gcggccgggt cccggtcaaa
     1201 gcaggcctta aaatcgcggt agatggcgta aaaagggttt gtcatgatgg cgatcattgt
     1261 aaaacataac ggtgggttta tctacgattt agtaaggact tcaggggtta tcgcgtaagg
     1321 agtacgtcga aaattatcgt tccgtgcgct gagtgtccgt acaccgtatc cgattttcgg
     1381 tcaaaccgaa aaacgaaaaa gcatggtgtc gccgtcccgg acctggtagt ctttcccctc
     1441 aatcctcaaa agaccctttt ccttcacggc cgcgggggtt ttatactgaa gcaaatcttc
     1501 acagcgcatc acctccgcgc gaatgaaccc gcgctcaaaa tccgagtgga tcacgcccgc
     1561 ggcttcggga gccttggctc cgtttcggac ggtccaggcg cgaacctctt tttctccagc
     1621 agtaaaatac gtgacgagat tgagagccct gtagccggca cgaataagcg aatgaagacc
     1681 ggactcagcg agccctaatt ccttgagaaa agcttgctgg tcttcctcgc tcaggtcgat
     1741 gagctcggat tcaacttttc cggaaatgac aacgacttcc gcgttttctt ccgccgcttt
     1801 tttccgcacc tcatcaacgt gggggccacc tgacgggagg tccgtttcgg cgacattggc
     1861 cccgtaaagc acagttttca gcgttaaaaa ggccatatcc ttaagaagtt ttttttcttc
     1921 atccgtaaat ccgacacggc gaagcggcgt tccggcttca agcgctgtct tcactttcgc
     1981 gtaaaccggc attctttctt ttgcgtcctt gtctcccgtc ttcgccagtt tttcggtttt
     2041 ttcataccgc gcgttgatcg tgtccaggtc tttgagacaa agctcggtgt caatcacctc
     2101 aatgtcccgc accggatcga ccgtggaggc aacatgcaca atgttggggt cctcaaaaca
     2161 acgaacgaca tgaaggatgg cttcgacctc cttgatgtgt ccgagaaact ggttcccaag
     2221 tccttccccc ttggacgcgc cggccacaag tcccgcaata tcgtaaaact cgacggtcgt
     2281 gggtgtggtc ttttggggat ggtaaagatt tgacagttcc gtcagccgag gatccggcac
     2341 gggaactatt ccgacattgg gctcaatggt acagaacggg tagtttgacg ccgcggcgtg
     2401 cgccttggcc aaagcgttaa aaatcgtgga tttacctacg ttgggaagcc caacaatacc
     2461 gcatcgaaag cccatagtgc ccggcactat agcaaagaac actgatgaat acaacccagc
     2521 aagtttcttt tttcatcaaa atcagattct gattctggcg gttttcagac tatgaagaaa
     2581 aagaaatggg aacttgacaa tattatataa tatgtatata ttttatgata aatatgaata
     2641 tctggaggcc ccatgttccc tctaaaaaga aagatatgtt tgcctgtttt cacaatctgg
     2701 tggatggttt cgacgttcca accgttggcc gaatcgtcgt ggacaaaacc gcttcagcct
     2761 tctttcgcgg gaggcgagct cgtttgtttg gccgctcatc cgaaggacca tacacgcttt
     2821 cttgtggcta atcgctccca gatttttgaa ggagcttcgt ctttagactg gcgcatgcta
     2881 tggtcatcga ccggccgtca ggcaaacatt aaaaaaatcc tcacgtttcc gctccttccg
     2941 gaccaaatct ttgtcctgac ccctgaagaa atcttgatgg gagatctccg ttcaggagcg
     3001 tgggcctcgt tttattcaag cgggggtaac cccgataaaa gtccactttc gtttactccc
     3061 gaccctgcca accccaatct ctggttcgcg ggcacttcca ggggcctttg gaccaccgaa
     3121 aattccggga aaacctggtc tcgctccgaa ctcgtcccat ctgaaaaagc cgttccactg
     3181 cttcattacg cggaagaccg attgttcatc ggaagcgaaa actccattta tcttgtccag
     3241 cgtgaaacct caccaaaaca cgtttttgaa ctccctcaaa aagaaaagga tttctctgaa
     3301 gccgaagaag cgttgaaaga caagacggct ttttatcacg ccaggatttt tgaactgatc
     3361 gaagctccgg tctcaagcgg tcatttcttt ctcgggaccg atgacggcgt ttttgaaacg
     3421 tccgacggag ggctaaactg gtttcccctt cctcaaagcg gcctgcagag cgctgtcatt
     3481 ttggatcttg ttttttcaga aaaaacaaat cttctttttg ccgcgactcc ccggggtgtt
     3541 tttcaatatc aagccaacaa gaaatcctgg cgggagctct cccggggact tgcccgatcc
     3601 aactcgagaa gtctcgcaat catgaatgag tcgaccctcg cggccatcac gggagacggc
     3661 tttgttcaat attccctttt cccggagaca cccgagccca cgtccttatt tatgcccgga
     3721 gcagaaactc ttaatctttt cagaaaactt gtcgtcctgg aaccttccgc ccgggatatc
     3781 cagcgcgaag cgatccgtta cgccaatgtt tctaactgga aaacccggcg gtggcatgtc
     3841 gcgtcacgag ccgccgcgct gatcccttcc ctttcgttcg ggaaaacgat gtaccgcaac
     3901 tcatcgctca gcacttacgg cggaaaattc ttcgcaggcc ccgaagacgt caacaaaacc
     3961 tttgataccg atatacgctg ggatctcggt aacgcccttt tcagttcgtc gcaaacttct
     4021 atcgattcac gggagaaact catggtcgat cttcggcgtg acatcctttc cgagaccacg
     4081 cgtatctatt acgaaagaag acgtctccag attgatctca tttacacgcc ctctctttcg
     4141 gagcaag
//
LOCUS       DAOSIO010000152         4119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_19128_length_4119_cov_3.408711, whole genome shotgun sequence.
ACCESSION   DAOSIO010000152 DAOSIO010000000
VERSION     DAOSIO010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19128_length_4119_cov_3.408711"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4119)
                     /locus_tag="PK590_06055"
     CDS             complement(<1..>4119)
                     /locus_tag="PK590_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24192.1"
                     /translation="DGSINKDLAFDFAKDAQALAQHQTGLTDGPGFRFVDQSGAGESF
                     ELKLAAQGLAVKALDVKGMGGTVLGDLSVNIASDTADFAKVADAARMKLDRMRAQAAD
                     FDLPRDATDRESLSQVGKLLNALQSIEGTGEAGLPPIAQEVSLEVKGDQDIPFGTFKL
                     FSNDEFSVQNVKIFGKNNSFMEVSKKFLDESQAMDYIHKLQEKAGEYDLFDHSPRSLS
                     EANKKGSQAFLDEVVKPGFAQLGDSANPLFVQMANSNLAIQSQGLLMKYQADLSGITA
                     QKVSPQDPAVLQLTGVAQRGSVLQPPADTPAMRAESSIRIDGSRAETALDPKNIANVP
                     QIFETWKAEELASIESLPNGMSLTHDIVDFSGATPQVLWRMVLAKNDNGQLIGGFSAP
                     NASEVYLQKTPNEKFKKYSAAQESPGRITIAQEPPVAKAVADAGGSATEPTPTATPAP
                     AVSAEPELLDVPSGLNALGQMSRTQIMEKIQAASVVEPPNTMQDAGGNLYSHLGDFVG
                     GKTKTCNMIINGKKLEVDVKYSLTAERTLDMTITGIRSMDSTDVTLPIKGAVIDLLAR
                     GSAAEFLPDHWSLDIHFEGDTARTKTYARGNWRFTAQVKTNDGKVIRGAYREGVDPYG
                     NVKAPEAGSIKTANGRITSEGFDELKQWSANTGIEISSIEGSRKVYQFQSFGVLVFDK
                     RGGLVDVYGYENKAYVSVQQTPGQATVRTADGPKPLNYKAFYELKGAVGGESGTVRLY
                     VDSRRQQVGMSFGKSGGTQKFLSYSVNKLEYSLCSTATLDDQIFTVSTQDGKIIGYLD
                     AVGLKTVRRDATQIQSAGGSGPAHQLKLASAEKDPGSDSNKTLTILFDRDEKPVFYQS
                     AATISFLNEKGLAGEKFFVGLTGQNVWMQTQNESFREFHIVSEFVATKTLTTCDGQSM
                     TITHMTPKLGWGVGDGMPDSRDQSAAIRALTAIGAFRESFSAVLDGRPVILLGVTDMD
                     NFVALAENDRGGFWVHVEKDGKATKPPVLYKPGEKIIAPAKSLAPQSAVGKVASEMGA
                     SQVSVSGKFYEANGTTEISLNNEKFHEYAAILGLTAVFSGLHDGASQFENCQIAFAHM
                     NYSGSEMKNGFVPIFDADGKKVSGSLHYENMHGNESSVRTVGWEKVENKVKLVNRKEF
                     QEEAVRQTVSFFDLKNPGTKISEGNEYQAMLSVFASNLPQAGLFNGLILTDTGGMQWR
                     WTADKFDFNVGDMKADATVASLDGERGWQLDKGKYKLGDVRRETITGNDTKAWADVDG
                     VTKMTQTYNVKVKNVEIRKLNSVILLSDGSTCTEEWALKSATSIGWGTSITVNLTDGN
                     SLRASGIHTLEEFKKPETVLHGTGFYSNVIVGRDDQGYQLYYAGHENGTTSKY"
BASE COUNT          904 a         1192 c          917 g         1106 t
ORIGIN      
        1 atacttggat gtcgtgccat tctcatgacc cgcgtaataa agctggtagc cctggtcgtc
       61 acgtcccacg atgacattcg aataaaagcc ggtaccgtgg agcacggttt ccggtttttt
      121 gaattcctcg agtgtatgaa tccccgaagc tcgaaggctg tttccatccg tcaggttgac
      181 cgtaatcgaa gtgccccatc cgatgcttgt tgcggatttt aaggcccatt cctctgtgca
      241 agtgcttccg tcagacagta gtatcacact attcagcttc ctgatttcga catttttcac
      301 ctttacgttg taagtttggg tcatctttgt taccccgtct acatcagccc aagcctttgt
      361 atcgttgcct gtaatcgttt cccttcttac atcccccagt ttatatttcc ccttatcaag
      421 ttgccagcct cgctcgccgt ccagagacgc cacggtagcg tcagctttca tatccccgac
      481 attaaaatcg aatttgtccg ccgtccagcg ccactgcatt ccaccggtat ccgtcagaat
      541 caggccgttg aacaatcctg cctgcggaag gttactcgca aatacgctca gcatcgcctg
      601 gtattcgttg ccttcggaaa tctttgtccc cggattcttg agatcaaaaa aacttaccgt
      661 ctgccgaacc gcttcttcct gaaattcttt acggttcacc agtttgacct tgttttccac
      721 cttttcccag cccacggtcc gtactgaaga ttcgttgccg tgcatgtttt cgtagtggag
      781 tgatccactc actttctttc cgtccgcgtc gaagattggc acaaacccgt tcttcatttc
      841 cgaccccgaa taattcatat gagcgaacgc gatttgacaa ttctcaaatt gagaagcgcc
      901 gtcatgcaat ccgctgaaaa ccgccgtcaa tccaaggatc gccgcgtact cgtggaattt
      961 ctcattgttc agagaaatct cagttgttcc attcgcttca taaaatttcc ccgaaactga
     1021 aacctggctc gcgcccatct cactcgcaac tttgcctacc gctgattgag gggcaagact
     1081 ttttgccggc gctataatct tttcccccgg cttgtacaga acgggcggtt ttgtcgcctt
     1141 cccgtctttc tcaacatgga cccaaaatcc ccccctatcg ttttcagcca gcgccacaaa
     1201 attgtccata tccgtgacac ccagcaagat aaccggccga ccgtctagta ccgcggaaaa
     1261 agattcacga aatgccccga tggccgtcaa cgctctgatc gcggcacttt ggtcccttga
     1321 atcgggcatt ccgtcgccga cgccccaccc aagttttggc gtcatgtggg tgattgtcat
     1381 cgattgaccg tcacacgttg tcagagtttt tgttgccaca aattcactca caatgtgaaa
     1441 ttcacgaaag gactcgttct gggtttgcat ccacacattt tgccccgtca gccccacgaa
     1501 aaatttctct cctgccaaac ctttttcatt gagaaaactt attgtggccg cactctggta
     1561 aaaaacaggc ttttcgtcac ggtcaaaaag gatcgtgagg gttttgttac tgtcggatcc
     1621 agggtctttt tcggcgctgg caagtttgag ctgatgggca ggtcccgagc cgcccgcgga
     1681 ttgaatctgc gtggcgtcac gcctgaccgt cttcaatccc actgcgtcca ggtaaccgat
     1741 aatttttcca tcttgggtcg acacagtgaa aatctgatcg tccaacgtgg ccgtcgaaca
     1801 caggctatac tcgagtttgt tgacgctgta cgagagaaac ttttgagtgc cgccggattt
     1861 tccaaaactc attcccacct gctgcctgcg gctatctaca tacaatcgaa ctgtccccga
     1921 ctcacctcct acggcgccct tcagttcata aaacgcttta tagttcagag gcttcggccc
     1981 atccgctgtt ctgacagtgg cttgacccgg ggtttgttgg acagaaacgt aggctttgtt
     2041 ttcataccca taaacgtcca caagaccacc tctcttgtca aaaaccagaa ccccaaacga
     2101 ctgaaactga tacacctttc gagaaccttc aatcgatgag atctcgatcc cggtattggc
     2161 agaccattgc ttaagctcat caaagccttc ggaagtaatc cgtccattgg cagttttaat
     2221 cgagcccgcc tccggtgctt tcacgttccc gtacggatcc actccctccc ggtaagcgcc
     2281 gcggatcact ttcccgtcgt tcgtcttcac ctgggcagta aaccgccaat ttcctcgagc
     2341 ataggttttt gtacgggctg tatcgccttc aaaatgaatg tcaagactcc agtgatccgg
     2401 tagaaattcg gcggcggatc cacgagccag aagatcgatg accgcccctt tgatcggcag
     2461 tgtcacgtct gtactatcca tggagcggat cccggtgatt gtcatgtcga gagtacgttc
     2521 agccgtcaaa ctgtatttaa catcaacttc cagcttcttt ccgttgatta tcatgttaca
     2581 tgttttggtt tttccaccaa caaaatctcc aagatgcgag tagagattcc caccagcgtc
     2641 ctgcatagtg ttcgggggtt ccacaacgct tgccgcctgg attttctcca taatttgcgt
     2701 tctggacatc tgtccgaggg cattcaaacc cgatggcaca tcgagtagtt cgggttcagc
     2761 gctgaccgca ggcgctggcg tcgcagtggg tgttggctct gtcgcactcc ctccggcatc
     2821 cgcaaccgct ttcgcaactg gcggttcctg ggctatcgta atcctcccgg gtgattcctg
     2881 tgccgccgaa tactttttaa atttctcgtt cggcgttttc tgcaaataaa cttccgaggc
     2941 attcggggct gaaaatccgc cgatcagttg gccattatca ttcttggcga gtaccattcg
     3001 ccagagtact tggggcgtgg caccggaaaa atcaacaatg tcgtgcgtca gcgacatacc
     3061 attgggaagc gactctattg atgctaactc ttccgccttc caagtctcaa atatctgggg
     3121 aacattcgca atattttttg gatcaagtgc tgtttctgcg cgagatccgt caatccggat
     3181 cgagctttcc gctctcatgg ccggagtgtc tgcgggaggt tgaagcacgg atccccgctg
     3241 tgctacccca gtcagttgaa ggacagccgg atcctgcggg gatacctttt gagccgtaat
     3301 tcccgagaga tccgcctggt atttcatcaa aagcccctga ctctggatcg ccagattact
     3361 gttcgccatc tggacaaaaa gcggattggc cgaatcccca agttgggcaa aaccaggttt
     3421 gaccacctca tcaaggaaag cctgggaacc ttttttattc gcctcactca ggctacgggg
     3481 gctatggtca aagaggtcat attcccctgc cttttcctgt aacttatgga tgtaatccat
     3541 agcctgagat tcatccaaaa acttcttcga cacttccata aaactgttgt ttttcccgaa
     3601 gatttttaca ttctggacgg agaattcatc gttagaaaaa agcttgaagg tcccgaacgg
     3661 aatatcctga tcccccttaa cctcgaggct cacttcctgc gcgatcggtg gaaggccggc
     3721 ttcacccgtt ccctctatag actgcaaagc attgagaagt tttccgacct gggacaggga
     3781 ctcgcgatca gtcgcgtccc tgggaagatc gaaatctgcg gcctgggctc gcatccgatc
     3841 cagtttcatt ctggccgcat ctgccacctt tgcgaagtct gcagtatcgg aagcaatgtt
     3901 cacgctcaaa tccccaagaa ccgtcccccc cattcctttg acatcaagcg ccttcaccgc
     3961 taacccttgg gccgccaact tcagttcgaa agattccccc gcgccgcttt gatcgacaaa
     4021 cctgaaaccc ggaccgtccg ttagcccggt ctgatgttgc gcaagcgcct gcgcgtcttt
     4081 cgcgaagtca aaggccaagt ccttattaat tgatccgtc
//
LOCUS       DAOSIO010000153         4086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_19300_length_4086_cov_4.235177, whole genome shotgun sequence.
ACCESSION   DAOSIO010000153 DAOSIO010000000
VERSION     DAOSIO010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19300_length_4086_cov_4.235177"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..361
                     /locus_tag="PK590_06060"
     CDS             113..361
                     /locus_tag="PK590_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24193.1"
                     /translation="MKSKKKVRVGMVSLGCPKALVDSEIVLGALGEFRVAIARRVEES
                     DVVVLNTCGFIRDAKNESIDYILELIEQKKGEGCVPSS"
     gene            343..1446
                     /gene="rimO"
                     /locus_tag="PK590_06065"
     CDS             343..1446
                     /gene="rimO"
                     /locus_tag="PK590_06065"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOG24194.1"
                     /translation="MRAVIVMGCLVQRYAAELQKSFPEVDAFLGSGDYDQLTRVLKSV
                     LAGKRLTVTEHLGYLGKSDEARLTLTSKFSRYLKISEGCNHTCSFCAIPQIRGRFRSR
                     TIADVEKEAKLLVQEGAKEIILIGQDITQFGCDYAQKPMLSELLEVLERIDGLDWIRL
                     LYAYPNSVSDALIRRIAESRKICHYLDLPLQHISDNILAAMRRGFNRQKTRELIRKLR
                     SDIPDLTLRTSFIVGFPGETEKNFQDLLEFVSWARFERLGVFKYSREEGTRAAKMRPQ
                     VPEKVKERRYHELMTLQRGIAEEVSKRQVGREMNVLIEEKMVPDTWKGRTVMDAPEID
                     PHVIVMSSLALRKGTFYPVRITGAKGYDLLGKI"
     gene            1424..2440
                     /locus_tag="PK590_06070"
     CDS             1424..2440
                     /locus_tag="PK590_06070"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOG24195.1"
                     /translation="MTFSGKFKFSLFHPNHLSIFRGVIGGVLPFLIFANSPAAHLVAV
                     TLFILGAITDYWDGWLARQYKLESKFGKWIDPFMDKILILAPLAAFSKLGFFSPWWLV
                     PIFAREIVVTFCRTAWLLEGKSFGAEKLGKLKFVFQVASVFVAFGVFVFWDYASTEAI
                     ARWFAPLLLPVIALTVLLTLLSGFFFLWNQRGHFASQGFVRFVLACGVGLFPKAPGTW
                     GSLAGILLVFLTSWNLGLYLVSMVTLLVVGQEMFRRLEDKSDPDPQYVVIDEVVGILV
                     TFLLIPVTWVTVPIGFLLFRLFDVIKPFPIRKLERIPGYWGIMADDIGAGIYAWIILF
                     LIFA"
     gene            2451..3710
                     /locus_tag="PK590_06075"
     CDS             2451..3710
                     /locus_tag="PK590_06075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HOG24196.1"
                     /translation="MRPSAAILSVGNEVLRGMAVNSNAAFLGRELSDLGFDVTTNTTC
                     PDTEEAIKFHLGDLFRRTDVVILSGGLGPTPDDVTREGIASYFNVPLIFSKAQFSRFR
                     RIYKRFKMPVPLLVRREACYPANAKPLVNRFGIAVGFVIEEKGKLVIALPGVPSELIN
                     MYGDVVRPLLKKHYPRVESVNQLIVKMAGISEPDVMKKLRKDFFADRFVFGIYPTAGE
                     IAIRVQADSRKVLKKIKQKISKRLQNFIYAWDDLPLARVVGQLLEEREETLAVAESCT
                     GGLLSSEISRYAGTSHFFKGGAVAYHQEVKTQLGVDPRILRRHGEVSPEVAKALATAA
                     RSRMNSTYGIGITGIAGPQGGSPKKPVGLVFIAWAGPGRRPKVWRHHFWGDRRQIQRK
                     ATVKALEYLWRKIREQGPFSARKQVAG"
     gene            3722..>4086
                     /gene="thpR"
                     /locus_tag="PK590_06080"
     CDS             3722..>4086
                     /gene="thpR"
                     /locus_tag="PK590_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOG24197.1"
                     /translation="MRLFLGFPLTNFINEIEQVLAVLRPGLDGVKWVLPEQYHMTIHF
                     FGDIPVSEVEIIDKGLRPLFDKIPPPSIRLTGIGAFPNMRRPNVLWLGVEEPRGTLQE
                     IVLRVRQAVSQLGYPVEDGR"
BASE COUNT          936 a          915 c         1163 g         1072 t
ORIGIN      
        1 cgcaacctcc ggatcgacag tccggagctc taaccaattg agctacgccc ccacctcaga
       61 aagatcaccg aaagtggagc ggtattctaa ccatacgatg ggttcttctc aagtgaaaag
      121 taagaaaaaa gtacgagtcg gcatggtgtc gttagggtgc cccaaagctc ttgtggatag
      181 tgagattgtt ttgggggcgc ttggtgaatt ccgtgttgcg atagcgcggc gcgtcgaaga
      241 aagtgacgta gtcgtgctca acacctgcgg gtttatacgg gacgcgaaaa atgaatccat
      301 tgattatatt ttagagctga ttgagcaaaa aaaaggggaa ggttgcgtgc cgtcatcgtg
      361 atgggatgtc ttgtccagcg atacgcggcg gaattgcaga agagtttccc ggaggtcgac
      421 gcgtttcttg gctcgggtga ttacgaccaa ctgacaaggg ttttaaagtc agtcctcgcc
      481 ggaaaacgtt tgactgtgac cgaacacttg ggataccttg gaaaatctga cgaagcaaga
      541 ttaactctta cctcgaaatt ttcccgttat cttaagattt ccgaaggttg taatcacact
      601 tgctcgttct gcgcgatacc ccaaattcgg ggccgcttcc gctcgcgtac gattgccgat
      661 gtcgaaaaag aagcgaaatt gctcgtccag gagggtgcga aggagattat tctgattggg
      721 caggatataa cccaattcgg atgcgactat gcccaaaagc caatgctctc ggaactcctg
      781 gaggttctgg agaggatcga tggactggat tggatcaggc tactttatgc ttatcctaat
      841 tccgtaagtg acgcgttgat ccgaaggatt gccgaatccc ggaagatctg ccactacctg
      901 gacttaccac ttcagcatat cagtgataat atactcgccg cgatgaggcg tggttttaat
      961 cgtcaaaaaa cacgcgaatt gatccgaaaa ctcaggtccg atattcccga tctcactttg
     1021 aggacgagtt ttatcgttgg ttttccaggt gaaaccgaaa agaattttca ggacctttta
     1081 gaatttgtgt cctgggcgcg atttgagagg ctcggtgttt tcaagtattc gcgggaagaa
     1141 gggacgcggg cggcgaagat gcggccccag gtcccggaaa aagtgaaaga gcgcagatac
     1201 cacgaactga tgacccttca gcgcgggatc gccgaagagg ttagtaaaag gcaagtgggg
     1261 cgagagatga atgttttaat tgaggaaaag atggttcctg atacctggaa aggacgtaca
     1321 gtgatggatg cgcccgagat tgatccgcac gtgatcgtga tgagttccct cgctttacgg
     1381 aaaggaacat tttatccagt cagaataaca ggagcgaaag gttatgacct tctcgggaaa
     1441 atttaaattc tcattgtttc atcccaatca tcttagtatt tttcggggag tgatcggagg
     1501 ggtgttgccc tttctgattt tcgcgaatag cccggcggcc caccttgtgg ccgtcacgct
     1561 ttttattttg ggagcgatta cggattactg ggacggctgg ctcgcgcgcc aatacaagct
     1621 cgaatcaaaa tttggaaagt ggattgatcc gtttatggat aaaattctaa tcctggctcc
     1681 tctcgcggca ttttccaaac tcggtttctt ctcgccgtgg tggctggttc ccatttttgc
     1741 ccgggagatt gtggtgactt tttgcaggac ggcgtggcta ctcgaaggga aatcctttgg
     1801 cgctgaaaag cttgggaagc tgaagtttgt ttttcaggtg gcaagcgtct tcgtcgcgtt
     1861 cggggtgttc gtgttctggg attacgcgtc cacggaagcg atcgcccgct ggttcgcgcc
     1921 tttgcttctc ccggtaatcg ccctgacagt ccttctaacc ctgctttcgg gtttcttttt
     1981 tctctggaac cagcgggggc attttgcttc gcaagggttt gtccgttttg tcctagcctg
     2041 cggtgtcggc ctttttccaa aagcgcccgg aacgtgggga agtctggcag gaattttgct
     2101 tgttttttta acgagctgga accttggatt gtatctcgtt tcgatggtta ctttactcgt
     2161 ggtggggcaa gagatgtttc ggagacttga agacaagagc gatccagatc ctcaatacgt
     2221 tgtcattgac gaagtcgtgg gcattctcgt gacatttttg ctgatccccg taacgtgggt
     2281 gactgtgccg atcgggtttc tcctgttccg attgtttgat gtcataaaac catttcccat
     2341 ccgaaaactt gagcgtatcc cgggatattg gggcattatg gcggatgata ttggcgccgg
     2401 gatttatgcc tggataattc tgttcctgat cttcgcctga ggcccttttc gtgagacctt
     2461 ctgcggcaat tctttccgtc ggtaacgaag tcctgagagg aatggcggtg aactcaaacg
     2521 ccgcgtttct gggcagagag ctttcagatc tcgggtttga cgttacgacg aatacgacgt
     2581 gtcccgatac agaagaagcg atcaagtttc atttgggcga cctttttcgc cggacagatg
     2641 tggtgattct gagcggaggc ctgggtccga cgcccgatga tgtaacccgg gaaggaattg
     2701 cctcctactt taacgttcct ctgatttttt ctaaagcaca gttctcccgg tttcgccgga
     2761 tctacaaacg ctttaaaatg cccgtccctt tgctcgtccg tcgcgaggct tgttatccgg
     2821 ctaacgcgaa gcccctagtg aaccggttcg gcattgccgt gggatttgtg atagaggaaa
     2881 agggaaagct cgtgatcgcc cttccgggtg ttccgtcaga acttatcaat atgtacgggg
     2941 acgtggttcg accactgttg aagaaacact atccgcgagt ggagtcggtc aatcagctga
     3001 tcgtcaagat ggcgggtatt tccgagcctg atgttatgaa gaaactgcgg aaagattttt
     3061 ttgctgaccg ttttgttttt ggaatttatc cgacggccgg ggaaattgcc attcgcgttc
     3121 aggcggattc ccgtaaagta ctgaaaaaaa taaaacaaaa aatctcaaaa aggttgcaaa
     3181 attttatcta cgcatgggat gacctgcctc tggcccgggt tgttgggcaa ttgctggaag
     3241 aaagggaaga gacgctcgct gtcgctgaaa gttgcacggg aggattgttg tcttccgaaa
     3301 tatcgcgata cgcggggacc agccattttt ttaaaggggg agctgtcgcc tatcatcagg
     3361 aggtaaaaac tcagttggga gttgaccccc ggatccttcg aaggcacgga gaggtatcgc
     3421 cggaggtggc caaagcgctc gcgacggcgg cgcgatcccg aatgaatagt acctacggaa
     3481 ttgggattac ggggatcgcg ggtcctcagg gaggaagccc taaaaagccc gtgggccttg
     3541 tctttatcgc gtgggctggt cctggaagga ggccgaaagt ctggcgtcat cacttttggg
     3601 gagatcgcag acaaattcag cgaaaggcca cggtcaaggc gctcgaatat ctctggagga
     3661 aaatccgcga acagggccca ttttctgccc gcaagcaggt cgcgggatga ctgtgggcgc
     3721 cttgcgttta tttctgggtt ttccactgac aaacttcatc aatgaaatcg aacaggttct
     3781 ggcggtgttg cggcctgggc tggacggggt taaatgggtt ttaccggaac agtaccacat
     3841 gaccatccat ttttttggcg atatacccgt ttccgaagtg gaaataattg acaagggtct
     3901 gcgacctctt tttgacaaaa tcccgccgcc gtccatacgg ttgacaggga ttggggcgtt
     3961 tccgaatatg agacggccga atgttctttg gttaggcgtt gaggaaccga gggggacact
     4021 ccaggaaatt gttcttcggg tgcggcaggc ggtgagtcag cttgggtatc cggtcgagga
     4081 tggacg
//
LOCUS       DAOSIO010000154         4077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_19355_length_4077_cov_5.086027, whole genome shotgun sequence.
ACCESSION   DAOSIO010000154 DAOSIO010000000
VERSION     DAOSIO010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19355_length_4077_cov_5.086027"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..1184)
                     /locus_tag="PK590_06085"
     CDS             complement(147..1184)
                     /locus_tag="PK590_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HOG24198.1"
                     /translation="MEKILFLAHTQKDGSLPKIALEVLTAAQKLARDLAGSTFTVGLI
                     GGDVKVAADQIAGCGAEKILGVSGEDFSESRYATDVKAAEAIAKVVAATIVIAPGTSR
                     FSRALPGLSFRLKGMIDTHVSGINVNGAQLDIQRWYYRQRMVATLTRAARPWVLLVDS
                     GTFPVYSGPRGSGNVEPVQVALTEQDKRTKVTGVQAPLADAQTIRPDADLLFVAGAGW
                     TKKQSDGQTHGKEAENLILGFLKESKASLGSSKSLVDLGAEGETVLSFISHLNQIGQT
                     GSTPRHPKGLATCCHGEEPHVVGWRFINERRAINVDANCGWAQGKADVLYVADAFQVM
                     KKVNELLGRTA"
     gene            complement(1189..1998)
                     /locus_tag="PK590_06090"
     CDS             complement(1189..1998)
                     /locus_tag="PK590_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HOG24199.1"
                     /translation="MSTAYQIIVCGSLVPDPLQTLEPVQAATGPALKNEMMLPSVLDP
                     WAGHALFEAANLAKQNPGSKVTLLSLGPKAKLQQLMMTIAQKVPFELVVVDGSASGFM
                     DSTEVAAALAAAIEKIPGLDKTKLLVFGGCASASRDAGVTMQLIAEQLGISDVFLGVD
                     ELKVQPDGSLQLFERIEGGQYQVSTCACAPVVIGWATGNLPEPPNNPQVGMANMRGIM
                     PALQKAAPAGLKGDKIKFASVELPKQVRQTRIVKDTPVDEIANEIVTWMKG"
     gene            2193..2753
                     /locus_tag="PK590_06095"
     CDS             2193..2753
                     /locus_tag="PK590_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOG24200.1"
                     /translation="MIIALNKPYGVLSQFTKEAPNHRTLAEFGFPKDVYPIGRLDADS
                     EGLLLLSNEKRWTERLLKPYHAHKRTYHVQVEGTATEPALQKLRQGVLIQRMKTAPCQ
                     ARLMKDPGYPERNPPIRFRLTVPTSWIELRLIEGKNRQVRRMTAAVGFPTLRLIRVAI
                     GQLHLGKLAPGAWRELETREIDLLLR"
     gene            complement(2827..>4077)
                     /locus_tag="PK590_06100"
     CDS             complement(2827..>4077)
                     /locus_tag="PK590_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006920375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HOG24201.1"
                     /translation="EKSGKPTAAFPPGMDIPAPRTVVGDGPYGPVGQKLDQKFGLPEG
                     NHQREWALGMKAVVELPENTTLQPGTVIHTFGYPEPEIFGFFYVYPDRTVSIGIFIPS
                     WLDNPTRTGYRYLQHWMQHPVLWKHLNGATLKSWGAKSLQEAGVRGEPFIVGDGFARI
                     GEGSGSTNMLKGSGVDEAWTTGVQLAEGVIEILKAGKPFTKENLEIHYLGKRRSSWVE
                     KDGKVAKQARDGFSLSFELGMIGMGLAGFTNGLLNIPAKLKPTHARIPSLEKYYKGKV
                     SGEKIDAAFKEMARTNSSMHDAIMDAAGWPEIKYDGKLLISHQDALLLGGKVQAAEGY
                     ADHVVFLDAGKCQKCKTKICIEMCSGQAITAGPDGGVPLFDREKCIHCAACLWNCAYA
                     REESSDLANVDFRAGSGGLHSNIN"
BASE COUNT          928 a         1219 c         1013 g          917 t
ORIGIN      
        1 cctatgaacc ttttatcggc aaatcactta tgaatactta tgaaaccgct tctagaaaaa
       61 gcgggtctat ttcattcggg ctaccaggga aaaagcgtaa gttgcaaatt cacgcgctct
      121 gccaaaaact gcgactacag aaaaacttaa gctgtgcggc ccagtaattc attgaccttt
      181 ttcatgacct ggaaagcatc ggccacataa agcacatcgg ccttgccttg ggcccagccg
      241 cagtttgcgt ccacgttaat cgcgcggcgc tcattgatga aacgccatcc gacaacgtga
      301 ggttcttcac cgtggcaaca agtcgcgagc cctttgggat gtctcggcgt ggaaccggtc
      361 tgcccgatct ggtttaggtg actgataaac gaaagtaccg tctcgccttc cgctccaagg
      421 tccacaagag atttactgct tccaagagat gccttcgatt ctttcaaaaa acccaggata
      481 aggttctccg cctctttacc gtgcgtctgg ccgtccgact gttttttagt ccagccggcg
      541 cccgccacaa aaaggagatc cgcgtccgga cgaatcgttt gggcgtccgc cagtggcgcc
      601 tgaacaccgg tcactttcgt gcgcttgtcc tgctccgtca acgcgacctg gacaggttcc
      661 acgttcccgg aacctcgggg ccccgagtaa accggaaatg ttccggaatc gacaagaagg
      721 acccaggggc gcgccgcgcg cgtaagagtc gccaccatcc gttgacggta gtaccagcgc
      781 tggatatcaa gctgggctcc attcacgtta atgccgctca catgcgtatc aatcatcccc
      841 ttgagccgaa acgaaagccc cggaagggct cgcgaaaaac gggaagttcc gggcgcaata
      901 acaatcgtcg ccgcgaccac tttggcaatc gcctcggccg ctttaacatc cgtggcatag
      961 cgggattcac taaaatcctc tcccgagacg cccaaaatct tttcggcgcc acatccggcg
     1021 atctggtccg ccgccacctt gacgtcacca ccgatcaacc caaccgtgaa agttgagccc
     1081 gccaggtccc tggcaagctt ctgcgccgcg gttagcacct cgagcgcgat cttgggaaga
     1141 ctgccgtctt tttgagtatg tgccagaaaa agaatttttt ccatagggtt aacctttcat
     1201 ccaggtaaca atttcattag cgatctcgtc gactggcgta tctttcacga tgcgcgtctg
     1261 acggacctgt ttgggtaatt ccacggaagc aaatttgatc ttgtcgccct tgagccccgc
     1321 gggcgcggct ttctgaagcg cgggcataat cccacgcata ttcgccatcc caacctgggg
     1381 attgttcggg ggctcgggaa ggttccccgt cgcccatccg atcacgaccg gagcgcaggc
     1441 gcaagttgac acctgatatt gcccgccttc aatacgctca aacaactgaa gtgaaccgtc
     1501 gggctgaact ttgagttcat caactccgag aaacacatcg gagatcccaa gctgttcggc
     1561 gatcaattgc atcgtcaccc ccgcgtcacg ggacgccgac gcgcatccgc caaacacaag
     1621 aagtttcgtc ttatcaagtc ccggtatttt ctcaatcgcg gcggccagcg cggcggcaac
     1681 ctccgtggag tccataaacc cgctcgcgga cccatccacg accacaagct caaacggaac
     1741 cttctgcgcg atcgtcatca tcagctgttg cagtttggct ttgggcccaa ggctgagtaa
     1801 agtcaccttg cttcccgggt tctgctttgc gagattcgcg gcctcaaaaa gcgcgtgtcc
     1861 ggcccacggg tccagcacgg aaggcagcat catttcattt ttaagcgcgg gcccggtcgc
     1921 cgcctggacg ggttccagtg tctggagtgg atcgggtaca agactgccgc aaactataat
     1981 ctgataagct gttgacatcg ctaaaaacct cctgtgaaaa ttgaaaaaat taggagcagt
     2041 ctcgcattat attcagcccg tccgctttcg gccagcatgt ttttcatctt tatcgttcct
     2101 tttcaatccg tcgcgcgcga tccgcgcgaa aaccccgcgt cttctgacaa gaatgtgttg
     2161 tcagcgtcct gaaattaatg tagaaaagcg tcatgattat cgcgttgaac aaaccttacg
     2221 gcgtactttc acagttcacg aaagaagcgc cgaatcaccg gactctcgcg gaatttggat
     2281 tccccaaaga tgtttacccc atcggccggc tcgacgccga cagtgagggg ctccttctcc
     2341 tgagcaacga aaaacgatgg accgagcgcc tcctcaagcc ttatcacgct cataaacgga
     2401 cctatcacgt ccaggtcgaa ggcaccgcga ccgaaccggc gcttcagaaa ctgagacagg
     2461 gcgttctcat tcagcggatg aagaccgcgc cgtgccaagc gcgacttatg aaagacccgg
     2521 gctatcccga gcgcaatcca ccaatccgat tccggcttac ggtccccacg agctggatag
     2581 aattacgttt gatcgaaggg aaaaaccgcc aggtccgccg catgaccgcg gcggtcggct
     2641 tccccactct ccgcctgatt cgcgtcgcga tcggccaact tcacctcggg aaactcgcgc
     2701 ccggcgcctg gcgtgaactc gaaacccgcg aaattgacct actactaaga taaaaaatgc
     2761 cgcgcctttt tgtttgtctt tttgagggag tggagagttt tctccgcggc gcatcaattt
     2821 ccggaattaa ttgatattgg aatgcaaacc cccggaaccg gctcgaaaat cgacgttcgc
     2881 cagatcggaa ctttcttcgc gcgcgtaggc gcaattccag aggcaggccg cgcagtggat
     2941 acatttttca cggtcaaaaa gaggaacgcc accgtccggg ccggccgtga tcgcctgtcc
     3001 tgaacacatc tcgatacaga tctttgtttt gcatttctgg cattttccgg cgtcaagaaa
     3061 aaccacatgg tccgcgtaac cttccgcggc ctggaccttc ccgccgagaa ggagcgcgtc
     3121 ctgatgcgaa atcagaagtt ttccgtcata tttgatctcg ggccagcccg ccgcgtccat
     3181 tatggcatcg tgcatcgacg aatttgtacg cgccatttct ttgaaggccg cgtcaatttt
     3241 ttcgccagaa accttcccct tgtaatattt ttcaagcgaa ggaatacggg catgggttgg
     3301 ctttaatttc gcgggaatat ttaaaagtcc gttcgtaaac ccggcgagtc ccattccgat
     3361 catcccgagc tcaaaagaaa ggctgaatcc gtcccgcgcc tgcttcgcga ccttgccgtc
     3421 tttttctacc cagcttgatc ggcgcttgcc gagataatgg atctcgagat tttcttttgt
     3481 gaaaggtttg ccagccttaa gtatctcaat aaccccttcc gcgagttgca ctcccgttgt
     3541 ccacgcctca tcgaccccgg aacctttgag catgttcgtg cttccggaac cttcgccaat
     3601 acgcgcgaaa ccatcaccca caataaaggg ctcgccccgc actccggcct cctgcaagct
     3661 tttcgcgccc caggatttaa gggtagcgcc gttcaaatgt ttccagagca cgggatgttg
     3721 catccagtgc tggaggtaac ggtacccggt tcgcgtcggg ttatcaagcc aagagggaat
     3781 aaaaattcca attgacacgg tgcgatccgg ataaacataa aagaaaccaa aaatctcggg
     3841 ctcaggataa ccaaacgtgt gaatgaccgt accgggctgt aatgtcgtat tttccggaag
     3901 ctcaacaacc gccttcatcc cgagcgccca ctcccgctgg tgattgcctt ccggaagtcc
     3961 gaacttctgg tcgagctttt gtcccacggg accatacggc ccgtccccca caaccgtacg
     4021 gggggcagga atatccatgc ccggcgggaa ggccgcggtc ggttttcccg atttttc
//
LOCUS       DAOSIO010000155         4077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_19356_length_4077_cov_4.604923, whole genome shotgun sequence.
ACCESSION   DAOSIO010000155 DAOSIO010000000
VERSION     DAOSIO010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19356_length_4077_cov_4.604923"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PK590_06105"
     CDS             <1..531
                     /locus_tag="PK590_06105"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="catalyzes the phosphorylation of
                     N-acetyl-L-glutamate to form N-acetyl-L-glutamate
                     5-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOG24202.1"
                     /translation="RDLERLGAAAVGLSGFDAEVLNVVKHTENGDVGFVGDVSSVNTI
                     PIKELTEFGRIPVIFPVGKGPDGEMYNVNADEAAANIAAAMKVQKFIVLTNVRGILRD
                     ATNDQTLIPSLRINDTQMLIDQGIISGGMIPKVKACIDSLQGGVQKAHIIDGRITHAL
                     LLEIFTDQGIGTEITL"
     gene            608..1813
                     /locus_tag="PK590_06110"
     CDS             608..1813
                     /locus_tag="PK590_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOG24203.1"
                     /translation="MKTTDVQKLYSEFILPTYTQMPVCIVKGKGSRVWDLEGREYLDF
                     FPGWGVSALGHCPPPVMNAIKRQARTLIHIPNNFLNMKQAELASVISKASYPARVFFC
                     NSGAEANEGAIKFAKKYGSETGRHEIITMKRSFHGRTLAAMTATGQEKFHHGFEPLME
                     KFLYADFNDLDSVKRLITPRTVAIMLEPIQGEGGIHVAKPEFMEGLRKLCDEKDMLLI
                     LDEVQTGMGRTGKMFAYQHYHGIEPDILTLSKALGGGVPIGAFVVNRKIRKEVLSAGM
                     HGSTFGGSPLVCAASLAVFKTIRKEKLLAHAVKMGKLLMAELEKMRKKYPVIEETRGR
                     ALMVALKLKEPSAPYAQEARAQGLLVNSTQGDVLRLLPALTVSAKEIRKAMGILNRVF
                     EKLSRNKEG"
     gene            1816..2745
                     /gene="argF"
                     /locus_tag="PK590_06115"
     CDS             1816..2745
                     /gene="argF"
                     /locus_tag="PK590_06115"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOG24204.1"
                     /translation="MFNSLISIQDLRSEEIKKLFELAAKLKQRPQDFRNVLEGRIFGF
                     IFEKPSTRTWVSFQAGIFSMGGGVIYLGPEDIKLGIREEVRDVARALNRYLAGVVMRT
                     FSHWTITEFRDYFEKPVINGLSDKEHPCQALADYMTLYEKIPARKNPVLAFVGDGNNV
                     LHSLLLVAAKLGASIRYATPKKYQPDPKIVRAARGIAAKTGADIQGFFDPAEAVKNAD
                     AIYTDVWVSMGEEAIRKKKLKDFQGMQVNKGLVAKAKKGALIMHCLPARRGEEITDSV
                     MESKNCVVFDESENRLHIQKAILLYLCHQGAIQ"
     gene            2742..3944
                     /locus_tag="PK590_06120"
     CDS             2742..3944
                     /locus_tag="PK590_06120"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOG24205.1"
                     /translation="MKKVILAYSGGLDTSCAVKWLQEQYGLDVICFSAFIGEVKDKTK
                     IIRRAEAAGALKTLVVDLKEEFASEYIMPALWAHARYEGKYPLATALGRPLIAKWLVE
                     VAEREGADYVAHGCTGKGNDQVRLEVGIRSLNPDLKIIAPLREWEFKTREEEIEYAKE
                     RNIPIDVTKKSIYSIDKNIWGIAIEAGILENPMLEPPEEAYVMTKAYDKTPKNPRYLE
                     IGFEKGVPVRLDGKRMPLVAMIEKLNEIAGEYGVGRFDMIENRLVGIKSREVYEAPAA
                     DVLLKAHEELEGFVMDREFLHYKRVLSEKYSELVYFGLWFTPLKEALDSFFASNQSRV
                     TGKVRIKLDRGHALCVGRESKFALYHEKLATYSEGDIFDRKAATGFMKIWGLPYEGMY
                     QKQRGRKA"
BASE COUNT         1051 a          865 c         1200 g          961 t
ORIGIN      
        1 cgcgatcttg agcggctggg ggcggcggcg gtaggccttt ccgggtttga tgccgaggtt
       61 ctgaatgttg ttaaacatac cgaaaacggt gatgtcggtt ttgtcggtga tgtctcatcc
      121 gtaaacacaa ttccgatcaa ggaattgacg gagtttggac gaattcccgt gatttttccg
      181 gtcgggaagg ggccggatgg tgaaatgtac aacgtgaatg ccgatgaagc ggccgcgaat
      241 atcgcggcgg ccatgaaggt ccagaaattt atagtgctga cgaatgtgag aggtattttg
      301 cgggatgcca cgaatgatca aactcttatt ccgtcgcttc gcatcaatga cacgcagatg
      361 ctgatcgatc agggcattat ctcgggcggc atgatcccga aagtgaaagc ttgcattgat
      421 tcccttcagg ggggagttca gaaggcacac atcatcgatg gccgtatcac gcatgcctta
      481 ctgcttgaga tttttaccga tcaaggtatt gggacggaaa taactttata acacaagccg
      541 cgggaggaac agccagggat ggcgagtttt ttctgcggtc ttcgttaccc gcaaagtgac
      601 aaaaaacatg aaaaccacag acgtacaaaa actgtatagc gaattcatat tgccaaccta
      661 cacccaaatg ccggtctgca ttgtgaaagg gaaaggttcg cgggtgtggg atctggaagg
      721 ccgggaatat ctggattttt ttccgggctg gggagtttca gctttgggac attgtcctcc
      781 tcctgtcatg aacgcgatta aacgccaggc gcggacgttg attcatattc ccaataattt
      841 tctcaacatg aaacaggcgg aactagcaag cgtgatttcg aaggcttcct acccggcgcg
      901 cgtatttttt tgtaattccg gcgccgaagc caacgaaggc gccatcaagt ttgccaaaaa
      961 gtacgggagc gagaccgggc gccatgagat cattacgatg aagcgatcct ttcacggcag
     1021 gactttggcg gcgatgacgg cgacgggaca ggaaaagttt catcatggtt ttgagcctct
     1081 catggagaag tttttgtacg cggacttcaa tgatctcgat tctgtgaagc ggttaataac
     1141 gcccaggacc gtcgcgatca tgcttgagcc aattcagggc gaaggcggta tccatgtcgc
     1201 caagcccgaa tttatggaag gacttcggaa actttgcgat gaaaaggaca tgcttctgat
     1261 actcgatgaa gtccagacgg ggatggggcg gaccgggaag atgtttgctt atcagcatta
     1321 tcacgggatt gagccggata tcctgactct ctcgaaggcg ctgggtggtg gtgttccgat
     1381 cggcgcgttt gttgtaaacc gaaaaatccg gaaagaagtc ctttcggcag gcatgcatgg
     1441 ttcgaccttt ggcggtagcc cgctcgtttg cgcggcaagt cttgccgtct tcaaaacaat
     1501 ccgcaaggaa aagctgctgg ctcacgctgt gaaaatggga aaacttctca tggccgagct
     1561 tgaaaaaatg cgaaaaaaat atccggtcat cgaagaaact cggggaaggg cgcttatggt
     1621 cgcgttgaag ctcaaggaac cctccgcccc ctacgctcag gaagcaaggg cccaagggct
     1681 cctggttaat agcacacagg gtgatgtgct ccggcttctt ccggccctga cggtatccgc
     1741 gaaggaaatc cggaaggcga tgggcatttt gaatagagtg tttgaaaagt tgtccaggaa
     1801 caaagaaggc tgataatgtt taactcgctg atttccatcc aggaccttcg ttctgaggag
     1861 atcaaaaaac tcttcgagct ggcggcgaaa ctcaagcaaa ggccgcaaga tttccggaat
     1921 gtccttgagg ggcggatttt tggatttatt tttgaaaaac cttccacgcg gacctgggtg
     1981 tcgtttcagg cgggaatttt ttcaatggga gggggcgtga tttacctcgg accggaagat
     2041 attaagctcg gaatccggga agaggttcgg gatgtcgcgc gagcgctcaa tcgctacctg
     2101 gcgggggttg tgatgcgtac cttttctcac tggaccatca cggaattccg ggattatttt
     2161 gaaaagcccg ttatcaacgg gttaagtgat aaagaacatc cttgccaggc cctggcagac
     2221 tatatgactc tctacgaaaa aattccggcc aggaaaaatc ccgtattggc ttttgtcgga
     2281 gatggcaaca acgtgcttca ctcgcttctt ctggtggccg ctaaactggg ggcaagcatc
     2341 cgttacgcga caccgaaaaa gtatcagccg gatccaaaga ttgtaagggc ggctcggggg
     2401 atcgccgcga aaacgggggc tgacatccag ggcttttttg atccggcgga ggctgtgaag
     2461 aacgcggatg ctatctacac ggatgtctgg gtcagtatgg gggaggaggc aattcggaag
     2521 aaaaaattga aagattttca ggggatgcaa gtgaataagg ggctcgtggc gaaggccaaa
     2581 aaaggagctc tgattatgca ttgtttgccg gcgcgtcggg gagaagagat tacggacagc
     2641 gtgatggaga gtaaaaattg cgtggtgttt gacgaatccg aaaaccggct tcatatccag
     2701 aaagctattt tactttatct ttgtcatcaa ggagctattc aatgaaaaaa gtgattttgg
     2761 cgtattcggg tggtctcgac acatcttgtg cggtgaaatg gttgcaggag cagtatgggc
     2821 ttgacgtgat ttgcttctcg gctttcatcg gagaagttaa agataaaact aagattattc
     2881 gtcgggccga agccgcgggc gctctcaaga cgttggtcgt ggatcttaaa gaagagttcg
     2941 cgagtgagta cattatgccg gctctttggg cccacgcgag gtatgaagga aaatatccgc
     3001 tcgcgacggc gctgggaaga ccgctgattg cgaagtggtt ggtggaggtc gcggaacggg
     3061 aaggcgcgga ttacgtggcg catgggtgta cgggaaaggg gaacgatcag gtccgccttg
     3121 aggtcggcat cagatcactc aatcctgacc tcaagattat cgcgccgctc agggaatggg
     3181 aattcaagac gcgcgaagag gaaattgaat acgcgaaaga aagaaatatt ccgattgatg
     3241 taacgaagaa aagtatttac tccattgata aaaacatctg gggaatcgcg atcgaagccg
     3301 gtattcttga aaacccgatg cttgagccgc cggaagaagc gtacgtgatg acgaaggctt
     3361 atgacaagac cccgaagaac ccgcggtatc ttgaaatcgg ttttgaaaaa ggagtcccgg
     3421 tccggctgga cgggaaacga atgccgcttg ttgcgatgat cgaaaagctg aacgagatcg
     3481 cgggggaata tggtgttgga cgttttgaca tgatcgagaa caggctggtt gggatcaaaa
     3541 gccgcgaggt ttatgaggcg ccggcggccg atgtgttgct caaggcccat gaagagctcg
     3601 aagggtttgt gatggaccgg gagtttcttc actacaagag agtactgagt gaaaaatatt
     3661 ccgaacttgt ctacttcggt ctttggttta cgccgcttaa agaggctctc gattcgtttt
     3721 ttgcatccaa tcaatcccgg gtgaccggaa aggtgaggat caaattggat cggggacacg
     3781 ccctttgcgt gggcagggaa tccaaattcg cgctttatca tgaaaagctc gcgacttatt
     3841 cggagggtga catttttgat cggaaggcgg caaccggttt catgaaaatc tggggtcttc
     3901 cgtacgaagg aatgtatcaa aaacagcggg gacgtaaagc gtagaagatt tttccgccga
     3961 gtgaaaagtt cggtttcaca agtgttttcg atccggcgtt ttccaagacg tcgcaaacaa
     4021 caagcagggt ttccggaaga ttgctttcaa acgatagggt gattgatgtg acaaaga
//
LOCUS       DAOSIO010000156         3949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_20187_length_3949_cov_4.586030, whole genome shotgun sequence.
ACCESSION   DAOSIO010000156 DAOSIO010000000
VERSION     DAOSIO010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20187_length_3949_cov_4.586030"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..361)
                     /gene="trxA"
                     /locus_tag="PK590_06125"
     CDS             complement(35..361)
                     /gene="trxA"
                     /locus_tag="PK590_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOG24206.1"
                     /translation="MSQEKVFTDANFQTEVLQSAQPVLVDFWAEWCGPCRMLGPVVEK
                     IAAANAGKITVGKMNVDENPGTPSKYGIQGIPTIIFFKGGQPVQQLVGYQSQDKIQKM
                     IDEILR"
     gene            complement(597..839)
                     /locus_tag="PK590_06130"
     CDS             complement(597..839)
                     /locus_tag="PK590_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24207.1"
                     /translation="MFQEDDEPKEWEMKEEEPPRVLKEKWARDEILGPETVMCPSCKK
                     RVSADNMYCLYCGARVLQKAGFLSGLFVRIKSFFGK"
     gene            complement(1107..1709)
                     /locus_tag="PK590_06135"
     CDS             complement(1107..1709)
                     /locus_tag="PK590_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOG24208.1"
                     /translation="MRIFFDTETTGLPKNWREPPENSSNWPHIVQIAWAQYDGAGAQT
                     SFKSYIIRPEGFVIPPAAFRVHGISTERAIREGALLLDVLGEFKTAVEGSKLLIAHNM
                     DFDEKMVRAELSRKRMPDFLQGIGKLCTMKRTADFCAIPGPYGNKWPTLAELHRKLFG
                     SDFEKQHDAGADVLCCAKCYFELERRSFFREPLKSVKVRC"
     gene            complement(1923..2348)
                     /locus_tag="PK590_06140"
     CDS             complement(1923..2348)
                     /locus_tag="PK590_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOG24209.1"
                     /translation="MKKFFPVILYGVLFVSGCASPGKFYPGEPRAAGSYLVVYGRESC
                     PECVKFKKSLDRAGMDYEYRDLGNESVKNDLYPRMKKAGMDLTYFLLPVVEVNGDFAA
                     RPSLGDVLDRYGQAASKPRHPTVPVVSSACREGESCFAP"
     gene            complement(2499..3473)
                     /locus_tag="PK590_06145"
     CDS             complement(2499..3473)
                     /locus_tag="PK590_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24210.1"
                     /translation="MTDDEYQKFLEEIKLQLPSNPKLREVFTKLTGIEVSGEPSADSR
                     PEASDSRSELSQASPGTVETLASMLRPGRKADLSRSEVRAIYEVTLRGDQDALVETLK
                     RIAEKQIAEASMSAGTARVTREMAVATIDMIIEALKAAGVKGNLTMIFDVSPDKNFME
                     ALQAVKDEIGTAVFTKGAVRNLDRKALEGVTVQTVGSLKSYKPLAAENAEAVPVLSQN
                     LGSDYFANEVLFGVGLDAGDVQGEPFLEVTENVLRMAVGLLKADLMTRGMDPEEINAK
                     LMEILFKLDTDSGRGVLSVNGRHLVVHREALQQMMVEYQATAEIQRAA"
BASE COUNT          947 a         1129 c          890 g          983 t
ORIGIN      
        1 gaagaggtgg tctccgaatt ttccctttcg cttctcagcg aagaatttcg tcgatcatct
       61 tctggatttt atcctgggac tgatacccga ccagttgctg gacgggctga ccgcccttga
      121 aaaagataat cgttggaatc ccctggatcc cgtattttga cggcgtgccc ggattctcgt
      181 cgacgttcat cttgccgacc gtgatttttc ccgcgttcgc ggcggcaatc ttttccacca
      241 cggggcccag catgcggcag ggaccgcacc actcggccca aaaatcaacc aggacgggct
      301 gggctgattg aagtacttcg gtttgaaagt tggcatccgt aaaaactttt tcctggctca
      361 taattccccc gttcgagacc ggatccgttc aaaccagaga tccgtcaatt gttttataag
      421 gataattctg cgattgtgac ctaaccgggt gatttgaaaa cgccacgggc atctccactg
      481 acctccggcg aagcaaacct gagcggcccg atttcgactc gcggccgtaa tatacaacga
      541 agccgctacc atgtccagaa atcgcccccc caaaatcccc gaacgtttcg cgcccgttat
      601 tttccgaaaa aacttttgat ccggacaaac agtccgctca gaaaaccagc cttttgaaga
      661 acccgcgcgc cgcagtaaag gcagtacatg ttgtcggcag acacccgttt tttacaggac
      721 gggcacatga cggtttccgg ccctaaaatt tcgtctctcg cccacttttc tttcaaaacc
      781 cgtggcggtt cctcttcttt catttcccat tctttgggtt cgtcatcttc ctgaaacata
      841 gaacagactc cgtggacaca aacaggtgac tgtcacccgc gcgggattcc ttgtcacgcc
      901 gtacacctca cgccttgtta tcctgtcgta aaatcaacct ttcttactaa aattccagag
      961 acacgttttt gctaccaacg gatctaaaac cctctggatc aaagacaaga taactctttc
     1021 gaaaaaatac cccgagcgat cacttatttc cttctccaac tatgaccctt ttcccttccc
     1081 ccgataaata ttttgaccct gaccggtcaa caccggacct tgactgactt taggggttcc
     1141 cggaagaatg accgccgttc cagttcaaaa tagcacttcg cgcagcacag gacatcagcg
     1201 cccgcgtcgt gctgtttttc aaaatccgag ccgaaaagtt tccggtgaag ttcagcgagc
     1261 gtcggccatt tgttcccgta cgggcccggg atcgcgcaaa aatccgcggt ccgtttcatg
     1321 gtgcaaagtt tcccgatgcc ctggagaaag tcgggcattc tttttctgga aagttcggcg
     1381 cgcaccattt tctcgtcaaa atccatgttg tgggcgatca aaagtttcga accttccacc
     1441 gccgttttaa attcgccgag aacgtcgagg agcaaagcgc cttcgcggat cgccctttcc
     1501 gtgctgatcc cgtgaacgcg gaaagcggcg ggcgggatga caaatccttc gggccggatg
     1561 atatagcttt tgaacgaggt ttgggctccg gcgccgtcat attgcgccca agcgatttga
     1621 acgatgtggg gccagtttga ggaattttcc ggaggttccc gccagttttt cggaagtccc
     1681 gtcgtttcgg tatcaaaaaa aattcgcatc tccgaagccc tttattcgct aacttccgtg
     1741 acacaccggg cgttctgaag ctgaagcggg cgccactgac tcccgggcaa aaaccggttg
     1801 ttgagatacc agcagcgcga ggacaagaat cactgaaaac gattttggaa gacgcatggg
     1861 cggcatttta agctgtcttc cggtttttga gaaattgttt ttcgttttgc ccgtaccccg
     1921 gatcaggggg caaaacacga ttctccctca cggcaggcgc tggacactac gggaaccgtc
     1981 ggatggcggg gctttgaagc ggcctgtccg taacgatcaa ggacgtcgcc cagggaaggc
     2041 cttgccgcga aatcaccgtt cacctcgacg accggaagaa gaaaataggt caaatccata
     2101 cccgcttttt tcatgcgcgg atacaaatcg tttttaacgc tttcgtttcc gaggtcccta
     2161 tactcataat ccatcccggc cctgtccaga cttttcttga acttgacaca ctccggacaa
     2221 ctttccctcc cgtaaaccac cagataggaa ccggccgcgc gaggttctcc cggataaaat
     2281 ttccccggag aagcgcaacc ggacacaaac agaactccgt acaggataac aggaaaaaat
     2341 tttttcatag gattcgtatc atggaacaat ttaaaaaaac tgtcaaccgg acgccacccg
     2401 agcgagagaa gagaggttgg caaaacaaga agttaacaaa acagcggatt taacagcgga
     2461 ttcaggaggc taacgcctct ccgcgaacgg cgtccgtgtt aagcggctct ctgaatttcg
     2521 gcagtggcct ggtattcgac catcatttgt tgaagagctt cacgatgcac aaccaggtgg
     2581 cgcccattta ccgaaagcac tccacgtccg gaatccgtat cgagtttaaa aaggatttcc
     2641 attagtttgg cgttgatttc ctcgggatcc atccctcggg tcatgaggtc agccttcaag
     2701 agaccgacag ccatccgcaa cacattttcg gtcacttcaa gaaacggttc gccctgaaca
     2761 tcccccgcgt ccaatccgac accaaaaagc acctcgttcg cgaagtaatc cgatccgaga
     2821 ttttgagaaa gcacaggaac agcttcggca ttctcggccg cgagaggctt ataactcttc
     2881 agacttccaa ccgtctggac cgtcacccct tcaagcgcct tacgatccaa atttcgaacc
     2941 gcgcctttcg tgaaaacagc agttccgatc tcatccttca cggcttgcaa agcctccatg
     3001 aagtttttgt cgggagaaac atcaaatatc atggtcagat tacccttcac tccagccgct
     3061 ttcaaagcct ctataatcat atctatcgtc gcgacagcca tttcccgagt tactcgcgcc
     3121 gtgcccgcgg acatactcgc ctcagctatc tgcttttccg cgatccgttt caaggtttca
     3181 acaagcgcgt cctgatcgcc acggagagtc acttcataaa tcgcgcgaac ctccgatctg
     3241 cttaaatcag cctttctgcc aggccgcaac atacttgcca aagtctccac ggttcccgga
     3301 gacgcctgac tcaattcgga acgggaatca gaagcttcag gacggctatc cgccgaaggc
     3361 tcgcccgaga cttcaatccc agttaacttg gtgaacactt ctcgcagttt cggatttgat
     3421 ggtaattgaa gtttgatttc ttctagaaat ttctgatatt catcgtcggt cattggccga
     3481 gaagaaatcc cgctgtagct taataccatt ttacgcgttc tttgcccata ttctccgacc
     3541 tctttgacaa ccaaatatct ttcttggcct ttatttaatg ccgccgcata tcttttccca
     3601 tcacgcctcc ccgtcgcaat atcagatgtg tcgtttataa aagcttccgc tttgccacta
     3661 gccttatatg gaataaaagg ctcgggaatc tccacaacgg gtaaagaatg ctgtgtagct
     3721 agttgcttga gtgtgcgaac tgtttccgga tctgctatat actcccccga aggaaggact
     3781 ttcaagaata caccctgtat ctcgacgcgc tggtttggat ggtcgggatg ggttccagta
     3841 attaggactt cattatagag agtctccgtt gttctctcca gcaactcgtc cggccccagg
     3901 agactacgat ttactagtct ttcaactaca acgtccctcc ccccccccc
//
LOCUS       DAOSIO010000157         3949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_20189_length_3949_cov_4.350026, whole genome shotgun sequence.
ACCESSION   DAOSIO010000157 DAOSIO010000000
VERSION     DAOSIO010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20189_length_3949_cov_4.350026"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..2034)
                     /locus_tag="PK590_06150"
     CDS             complement(247..2034)
                     /locus_tag="PK590_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG24211.1"
                     /translation="MARKIRVEIVASAAFRSQPNWRSEFEQRLAYASRIFESEFKVKF
                     VPVKWREWPVVNEKASLRSLLEDLQSRFPLEKADIVIGLTSVKGVSLADVKDPDTVGQ
                     AKILSGYLVLRYPISPMYRIQEQTVLTHELGHLFGAVHTDEPSTVMFPIIDKQVPVRF
                     DSENHDIIMATRAIDFKRGVNALPRVAVQKLLGSYLKMAVRDQPFDFFYVIGALYLQL
                     GQYDDTLKSWKKAAEIMPDFPRIHYNLGMLYYQMGDHQNSVKELMQAVRSFRFPSERP
                     EELKALKALGDVFMSDNDLVSAYQAYTRAKSVSPNDKEVRLKLAVLQTRKGQLQDAIR
                     ELEALHYQDTNYVEAMLELGAAYYQAQRHGDSARMMKRVIEKAKKGSQPYLQAHSYLG
                     RIYGQSGQHKKAIDHFRTACAAQQSADCMKGLAQAYFDDGQINHCIATLAQVLMIQKE
                     DPDVYGTLAVALMQKGDYGKAYPLLEEGLKYAKDSRSESRFYQNMGHILIQTQKFDLA
                     KNNFQMGLSRDWSNLDCYFGLAMASIGLQDPLGAKRALEDVLRIDPQNKKATEMLRQV
                     EKILPQSAPIKIHLEGGSGQATFSIKSKK"
     gene            complement(2433..2837)
                     /locus_tag="PK590_06155"
     CDS             complement(2433..2837)
                     /locus_tag="PK590_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24212.1"
                     /translation="MSKIARVGLLSLCVVGFAAFNWGSSSTSGDSMDSKTNEPVAITD
                     VESSTAKAAVAASRILGSGTPEEKQARLESLRRLSESMAKYKQLQSTPVETSVSESPS
                     YGYSDEYSNEYSNSSAQKTVAPVSSPKKRGWF"
     gene            complement(3013..>3949)
                     /locus_tag="PK590_06160"
     CDS             complement(3013..>3949)
                     /locus_tag="PK590_06160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG24213.1"
                     /translation="QFVGRMIESVLSQSRLPDEVVLVDDGSTDATPSIVQSYAERFPI
                     VRLIQNSNQGPAASRNVAWRAAKADVVIFTDGDCVPERDWIEKLVKRFGPPEVAAVAG
                     TYRTLNTQNKLARFVGHEIAWRYRDVPAQVDAHGAYNLAIRKDVLEELGGFDESYKAP
                     SGEDWDLTYRISRKYKILFAPDAIVAHAHPESFWPYMKNQVRRGFDRIKLYNDHPEKM
                     KGDAYTGRLAKYQVLAAACFPLTLWMFFCKGWCFVPAALFLFLVGSGWSVFRFIYQRD
                     PSAARYGVGVQFARNFAWALGAVKGLLRFGIRRLW"
BASE COUNT         1006 a         1105 c          917 g          921 t
ORIGIN      
        1 ttatgaacag gttacaaaac aagcttaatt aattttaatt tttttcttca aaacatttaa
       61 aaaaaaccct ttcaaaagtc agctttttat atattcattc gaaatacttt ttaaacagat
      121 atataaaaaa ctttcattaa gtgagcttaa tttggaaact aaaatttgga ttaaattcgg
      181 aagttttcag gaccgataaa aaataaaggt aaggaactcc agcgtttccg attcaaagcg
      241 gatggattac tttttcgatt tgattgaaaa agtcgcctgg ccgcttccac cctccagatg
      301 aattttgatc ggggccgatt ggggaaggat cttttcgacc tgccgcaaca tttcagtggc
      361 ctttttattt tggggatcaa tccgcagaac gtcctccagc gcccttttcg cgcccagggg
      421 atcctgtaag ccgattgatg ccatggcgag cccaaaatag caatccaggt tagaccagtc
      481 acgggaaagg cccatctgaa aattgttttt agccagatca aacttttggg tctggatcag
      541 aatgtgtccc atattctgat aaaagcggga ctccgacctg gagtccttcg cgtatttcaa
      601 cccttcctcc agaagcggat acgccttccc gtagtcccct ttttgcatca acgcaactgc
      661 cagcgttccg taaacatccg ggtcttcctt ttggatcatc aaaacctggg ccagcgtcgc
      721 gatacaatga ttgatctgcc catcatcaaa gtacgcctgc gccaaaccct tcatgcaatc
      781 cgcgctctgc tgggcggcgc aagccgttcg gaaatgatca atcgctttct tgtgctgtcc
      841 ggattgccca tagatccggc caagataact atgagcctgg agatagggct gactcccctt
      901 tttagccttt tcgattacac gcttcatcat tcgcgcgctg tctccatggc gctgggcctg
      961 ataatacgcg gcccccagtt ccaacatggc ttccacataa ttcgtgtcct ggtagtgaag
     1021 ggcctccaat tcacgaatcg cgtcctgcaa ctgccccttt cgcgtctgga gaaccgccag
     1081 tttcaaccgg acttccttat cgttggggct tacggattta gcccgggtat aggcctgata
     1141 ggcggacact aggtcgttat cagacataaa aacatccccg agcgccttca gggctttcaa
     1201 ctcctcgggt ctttcactcg gaaaccggaa actccggacg gcctgcatca gttccttgac
     1261 ggaattttga tggtccccca tttggtaata aagcatcccg aggttatagt gaatcctcgg
     1321 aaagtccggc atgatctcgg ccgccttttt ccaggacttc agcgtatcgt catattgccc
     1381 gagctgaaga tagagggcgc cgatgacata aaaaaaatcg aacggttggt ccctgaccgc
     1441 catcttgagg taggagccca aaagtttctg aaccgccacc cggggaagcg cgttcacgcc
     1501 tcgtttgaaa tcaatcgcgc gggtcgccat gataatatcg tgattttcgg aatcaaaacg
     1561 gaccgggacc tgtttgtcga taatcgggaa cataaccgtg gagggctcat ccgtatgaac
     1621 cgctccgaac agatgcccga gctcatgagt gagcacggtc tgttcctgaa tccggtacat
     1681 cgggctgatc ggataccgca acacaagata ccccgagagg atcttggcct gtcccaccgt
     1741 gtcagggtct ttgacatcgg caagcgagac acccttcacg ctcgtgagcc caatcacgat
     1801 atcggctttt tcaaggggaa aacgggactg caaatcttcc agcaaggatc tcagggaagc
     1861 cttctcattg acgaccggcc attcgcgcca cttgaccgga acaaacttta ccttaaattc
     1921 gctctcgaat atccgggaag cataggcaag acgttgttca aattcagagc gccagttggg
     1981 ctgggaccga aacgccgccg aggcaacaat ttccacccga atttttcgtg ccatcacggc
     2041 cgaaacggcg gggctttcca attgggtgaa tatcagggca agaattagaa ggattcgcga
     2101 gaatcgaacc aaggctacaa gtgtccttcc gcggcaacgc gtatgacaag acgaaagtca
     2161 aacaccttga ttaactcctt tcagggactg gccccgtctg cgccaaatca aaatcagaga
     2221 ttagagattc aggacacccg gatcttcttt aattgctttg gctcaggtca gccaccattc
     2281 cgaagttgag gacaacaaaa acccgggcca gtctttatac tttgaagtct aatctcgcga
     2341 caaagaccgc ttcccagaag cgatctttgt cgcaaaacca gtaacgcccg ctcccccatt
     2401 caagggaaag cttctggcgc tcgctcaaaa acctagaacc aaccgcgttt cttcggcgaa
     2461 gacaccggag caacagtttt ctgagcagag gaattcgaat attcattcga atattcatcc
     2521 gaatatccat aagaaggcga ttcgctgacc gatgtttcga caggcgtcga ctgaagttgt
     2581 ttgtatttag ccattgactc gctcagacgt cttaaacttt cgagacgggc ctgtttttct
     2641 tccggtgtcc cggagccaag aatacggctg gccgcaaccg ccgccttggc agtcgaagac
     2701 tccacatcgg tgatcgcgac cggttcattc gtttttgagt ccatcgaatc gcccgacgtg
     2761 gacgaggaac cccagttgaa ggccgcgaag ccgacaacac aaagggataa caaaccaaca
     2821 cgcgctattt tgctcataac caacacctcc tggtcttatt ttgtctcatt aaaaaaaccg
     2881 cgacaccctc cgaataatcg ctcaacgcgg agtgatgata acaaaaatgc cttgccgagc
     2941 caagccggtt tttcccgccc cgtcctggag caaagtgttc gcctgccatt catttcgttc
     3001 tacactccgg gattaccaaa gccttcttat accaaaccgg agtagacctt tcaccgcacc
     3061 cagggcccag gcaaagttcc gcgcgaactg aaccccgaca ccatagcgag ccgccgaggg
     3121 atccctctga taaataaatc ggaacacact ccagccggaa ccaaccagaa acaagaaaag
     3181 agcggcgggg acaaaacacc agcctttgca gaaaaacatc caaagcgtta acggaaaaca
     3241 ggctgccgcc agcacctgat atttggcaag ccgcccggta taggcatcgc ccttcatctt
     3301 ttcggggtga tcattataaa gtttgattcg gtcaaacccg cggcgcacct gatttttcat
     3361 gtaaggccaa aatgactccg ggtgggcgtg agcgacaatc gcgtccggcg cgaaaagaat
     3421 tttgtacttg cgggaaatcc ggtaagtcag atcccaatcc tcgccgctcg gcgccttgta
     3481 agattcatcg aaaccgccca gttcctccag aacatctttc cggatcgcca gattataagc
     3541 gccgtgcgcg tccacttgcg cgggcacatc ccgataacgc cacgcgatct catgtccgac
     3601 aaaacgggct aatttgtttt gagtattcag ggtgcggtaa gtcccggcga ccgccgccac
     3661 ttctggaggt ccgaatcgct tcaccagttt ttcgatccag tcacgctccg gaacacaatc
     3721 cccatccgtg aaaatcacca cgtccgcttt cgcggcccgc caagccacgt tccgtgaggc
     3781 cgccggccct tgattggaat tttgtatcag gcgtacgatg ggaaaccgtt ccgcgtaaga
     3841 ctgaacgatc gaaggggtcg cgtccgtcga accgtcatcc accagcacga cttcgtccgg
     3901 aagccgactc tgtgataaca cgctctcgat catccgcccc acaaactgc
//
LOCUS       DAOSIO010000158         3910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_20433_length_3910_cov_3.975357, whole genome shotgun sequence.
ACCESSION   DAOSIO010000158 DAOSIO010000000
VERSION     DAOSIO010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20433_length_3910_cov_3.975357"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="PK590_06165"
     CDS             complement(<1..1109)
                     /locus_tag="PK590_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOG24214.1"
                     /translation="MVKAVINGIQVEVPAQTTILDAAKKVQIKIPTLCKHPDSYLHAS
                     AACGICLVRVKGSKKMLRACCTPIEEGMDIITHDPEIVAIRRTVIELFLSSHPNECLS
                     CGRNNNCELQTLVADFGIREEYFEKIVRDIPKDDSTGNIVLDPRKCINCGRCVDVCQN
                     VQDVWALTFLHRGFNTRMAPAGDISLSDSPCVRCGQCAAHCPTGAIFEKDDTVLAWRA
                     LRDPEQYCVVQIAPAVRVALGEAFGFPLGTNVTKKIYAALRRMGFKAVFDTNFSADVT
                     IMEEASEFIERFVHKKGPLPLITSCCPSWVDFMEKFHSDMIPHFSSAKSPHEMMGALI
                     KTYYAQKNNLDPAKLKVVSVMPCTAKKFEISRSAD"
     gene            complement(1151..2887)
                     /locus_tag="PK590_06170"
     CDS             complement(1151..2887)
                     /locus_tag="PK590_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOG24215.1"
                     /translation="MSKTVFIKDTGTAGKDRTRDFYGFFLGVIREKGLENEVIPVRVA
                     DIGLYNQGVVVRVVPDNFVYAQVEESDVSLIVDSIKTGKPYSAKLVKPVSKQLRIVLR
                     NCGVVDPESLEDYIAHDGFAALAKVLESYTPEKVIQEMKESGLRGRGGAGFPTGMKWE
                     FTRKSQSDQKYIVCNGDEGDPGAYMDRGVLEGDPYSVIEGMIIGGYAIGASKGLFYIR
                     AEYPVAIERIEKAIKRCHETGLLGNNILGSSFSFDLEIRLGAGAFVCGEETALIASIE
                     GKRGYPSPRPPFPSVKGLWGRPTCINNVETLANVPVILHKGADWFSKIGTETAKGTKV
                     FAVTGKVRNSGLVEVPMGTTVEDIVYGICGGAMRGKQVKAIQTGGPSGGVIPKEYFGT
                     KVDYESLQKMGSIMGSGGMIVMDEDDCMVDIAKFYLGFCVDESCGKCAPCRIGGTQML
                     KLLTKVTDGRGTQEDLARLEQISHAMQKASLCGLGQTAPNPVLSTLHYYRAEYDAHVN
                     ERKCPACKCANLLQFVIVQDKCVKCGMCFKACPVGAISGSRESGYLIDQTKCIRCGQC
                     FEVCKPKAISRS"
     gene            complement(2904..3251)
                     /locus_tag="PK590_06175"
     CDS             complement(2904..3251)
                     /locus_tag="PK590_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOG24216.1"
                     /translation="MAKLTREDLAKIKQDAAEAEKNWIKVGYSSCGIAAGAQEIFNVL
                     REEARKHNADIEIKKCGCVGMCSAEPIVEVCVKGLPRVHYGKVDRETAVKIIEDHVCG
                     KLLVNDHVMGIQG"
     gene            complement(3269..3778)
                     /locus_tag="PK590_06180"
     CDS             complement(3269..3778)
                     /locus_tag="PK590_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOG24217.1"
                     /translation="MDKSKIEKEIREIADAWKDKEGNLIMVLHQIQNRYGYVPREVSL
                     VLSEVLGVPLARIYEVITFYHFFRLSPPGKTTISVCMGTACYLKGGADLVAELEHLLC
                     IKPGETTKDGQFHLEVVRCLGCCGLAPVLKIGEKIYGGVKKKDIGNIIAHYSDEVGAG
                     TKTTETKAV"
BASE COUNT          950 a         1175 c          833 g          952 t
ORIGIN      
        1 atatccgcac ttcgggaaat ctcgaatttt ttagccgtac aaggcatgac ggacactacc
       61 tttaacttcg cgggatccag attgttcttt tgcgcataat acgtcttgat caacgccccc
      121 atcatttcat gcggcgattt cgcggacgag aagtgaggga tcatgtccga atgaaatttt
      181 tccataaaat caacccagga aggacaacag ctcgtgatga gcggaagcgg ccctttcttg
      241 tgaacaaatc gctcaataaa ttccgacgct tcttccataa tcgtcacgtc agcgctgaaa
      301 ttcgtatcaa aaaccgcctt gaaacccatg cgccggagcg cggcatagat ctttttcgtc
      361 acattagttc ccaacggaaa accaaaagct tccccaagcg cgacacgaac cgccggagcg
      421 atttgaacga cgcaatactg ttccggatca cgcagggcgc gccaggcaag aacggtatca
      481 tccttctcaa aaatagcacc cgtcgggcag tgagccgcgc actgtccaca gcgcacacag
      541 ggagaatccg aaaggctgat atcccccgcg ggcgccatcc gcgtattgaa tccccgatgc
      601 agaaaggtta aagcccagac atcctggaca ttttggcaga catcgacaca ccttccgcag
      661 ttaatacact tgcgcggatc cagcacgatg tttccggttg aatcatcctt cggaatgtca
      721 cgcacgatct tttcaaaata ctcctcccga attccgaagt ctgccaccag ggtctgcaac
      781 tcacaattat tgtttcgtcc gcaactgagg cactcattcg ggtgactgga caaaaaaagc
      841 tcgattacag tccgccggat cgcgacaatc tcggggtcgt gagtaatgat gtccatgcct
      901 tcttcgatcg gtgtgcaaca ggcgcgaagc atttttttcg aacctttgac ccggacaagg
      961 caaataccgc aggcggccga ggcgtgaaga taagagtccg ggtgtttgca aagcgtcgga
     1021 attttgatct ggaccttctt ggcagcgtcg agaatcgtcg tctgcgcggg cacttccacc
     1081 tggataccat taatcactgc tttaaccata tcaactccat caagttccta attagatttg
     1141 aaatttcaga tcagcttcgg cttatggcct tgggtttaca aacctcaaaa cactgtccgc
     1201 atcgaatgca cttggtctga tcaatcaggt aaccgctttc acggctcccc gaaatcgcgc
     1261 ccacagggca agctttaaaa cacattccgc acttcacaca tttgtcttgg acaatcacaa
     1321 actggagcag attcgcgcac ttgcacgccg gacatttcct ttcgttcacg tgcgcatcgt
     1381 attccgcgcg ataatagtgc aaagtagaca acaccgggtt gggagccgtt tgaccaagac
     1441 cacagagaga ggccttctgc atggcgtgtg agatttgctc aagccgggcc agatcctctt
     1501 gcgtcccccg tccatccgtc actttcgtga gaagcttcaa catctgggta ccgccgatcc
     1561 tgcagggcgc gcacttcccg caagattcgt caacgcaaaa cccaagataa aatttcgcga
     1621 tatccaccat acaatcatcc tcgtccatga caatcatccc gcctgagccc atgattgatc
     1681 ccatcttctg caggctttca tagtccactt tggtcccaaa atattctttc gggatcacgc
     1741 cgcccgaggg cccgccagtc tgaattgcct tgacctgctt tccgcgcatc gcgcccccgc
     1801 aaatcccgta cacaatgtcc tcaaccgtgg ttcccatcgg cacttccacc agtccggagt
     1861 ttctaacctt tcccgtcacc gcgaaaactt tagttccctt cgccgtctcg gttccgatct
     1921 ttgaaaacca gtccgcgccc ttgtggagaa tgacggggac attggcaaga gtttccacgt
     1981 tattgatgca ggtcggccgt ccccataacc ccttgactga cgggaaggga ggtctcggac
     2041 tcggataacc gcgcttgcct tcaatcgacg cgatcaacgc ggtctcttca ccgcacacaa
     2101 acgcgccggc gccaagacgg atttccagat cgaagctgaa ggaagaacca agaatgttat
     2161 tcccgagaag tccagtttca tgacaacgct tgatcgcttt ttcaattctt tcgatcgcga
     2221 ccggatattc cgcgcgaatg taaaaaagcc ccttggacgc accaatcgcg taacctccaa
     2281 tgatcattcc ctcaatcacc gaatacggat cgccctcaag gactccccta tccatataag
     2341 cgcccggatc accctcgtca ccgttacaaa caatatactt ctggtcgctc tgggacttgc
     2401 gggtaaattc ccatttcatg ccggttggaa atccggcgcc acctcgccca cggagcccgc
     2461 tctctttcat ttcctgaatg actttctccg gcgtgtaact ttcgaggacc ttcgcaagcg
     2521 cggcaaatcc gtcgtgagcg atataatctt ccaaactttc aggatcaaca acgccacaat
     2581 ttcgaagaac aattcttagc tgtttcgaaa caggcttcac cagtttcgcc gaataaggct
     2641 ttcccgtttt gatgctgtcc acgatcaggg aaacatcgga ttcttcgacc tgggcataaa
     2701 caaaattatc cggaactacg cggacaacca ctccctggtt ataaagaccg atatccgcga
     2761 cacggacagg gatcacctca ttttcaaggc ccttttccct gatcacgccg agaaagaaac
     2821 cgtaaaagtc cctggttcta tccttgcccg cagttcccgt atccttgata aatactgtct
     2881 tagacatagc gctcctgttt taactaacct tgaatgccca ttacgtgatc gttgacaagc
     2941 agtttgccgc acacgtgatc ttcgatgatt ttaacggccg tttcgcgatc caccttgccg
     3001 taatgaaccc ggggcagccc cttgacgcaa acctcaacga tcggctccgc cgagcacatc
     3061 cccacgcatc cgcatttttt gatttcaata tcagcattat gtttacgggc ttcttcgcga
     3121 agcacgttaa agatttcctg ggctcccgca gcaatcccgc aacttgaata acccaccttg
     3181 atccagtttt tttcagcctc cgcggcgtcc tgctttattt tggctaaatc ttcccgagtc
     3241 agttttgcca tgtctctccg cgctcccgtt agactgcctt cgtttccgtc gttttcgttc
     3301 ctgctccgac ctcatccgaa taatgtgcga taatatttcc tatatccttc ttcttgacgc
     3361 cgccatatat cttttcaccg atcttgagga ccggcgcgag gccgcaacag cccagacagc
     3421 gaactacttc aagatgaaac tgtccatcct tcgttgtctc ccccggcttg atacaaagaa
     3481 gatgctcaag ctcggccacc agatccgcgc cgcccttcaa ataacaggcg gttcccatgc
     3541 aaacagaaat ggtcgttttc ccgggaggag aaaggcgaaa aaagtggtaa aaagtgataa
     3601 cctcataaat acgcgccagc ggcacaccca agacctcgga aagcaccagg gaaacttctc
     3661 tcggaacata cccgtagcgg ttctggatct ggtggagcac catgatcaga ttgccttcct
     3721 tgtccttcca cgcgtccgcg atctcccgga tttccttctc aatcttactt ttgtccattc
     3781 aaaaactccc tcaaactgct tttatttttt atcgactcaa tcgcgtcagc caaccgcgtt
     3841 aaatgcgcaa gccccaaaca cttcatcctt cggccatccc gttcccaaca ttgccgaacc
     3901 gtgtcaagct
//
LOCUS       DAOSIO010000159         3903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_20477_length_3903_cov_4.141112, whole genome shotgun sequence.
ACCESSION   DAOSIO010000159 DAOSIO010000000
VERSION     DAOSIO010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20477_length_3903_cov_4.141112"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1022
                     /locus_tag="PK590_06185"
     CDS             96..1022
                     /locus_tag="PK590_06185"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOG24218.1"
                     /translation="MKALVTGGAGFIGSNLVRLLVEKKHSVTVLDNLSTGHLSNLGPF
                     PSVKFIKGDIRDERTISRALRGVDIVFHLAASVGNSLSIKAPVYDSETNVLGTLKLLR
                     AAHQNRIKKIVYSSSAGILGELKELPINENHPTNPISPYGVSKLAAEKMCLVYNSLYG
                     MKNVCLRYFNVYGINQRYDAYGNVIPIFARRIIRKQPLFVFGDGYQTRDFVNVIDVAK
                     ANYQAAITGKVAGAFNIASGASITINKLANMMMQTARTTVPLQHKKPRLGDVRDSLAN
                     ISAARKAFGFKPSVSLEEGLKKYMSWASQNRF"
     gene            1049..1948
                     /locus_tag="PK590_06190"
     CDS             1049..1948
                     /locus_tag="PK590_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG24219.1"
                     /translation="MKKKILILGATGMLGHVLMRHLSSLPGYEVYGTARNPAIVTRFF
                     PPDLADHFRHDTVDAINIDSVIRALAAVEPDIVINCIGLIKQLPIANDPLSAITINAQ
                     LPHRISLISKTAGARMIHISTDCVFNGQKGHYTENDPSNAEDLYGRTKYLGEVSYPHC
                     ITLRTSIIGHELEKGYGLVDWFLAQHGTVRGFTKAIYSGFPTIELARILSDYILPDEK
                     LSGVYHVSSDPISKFDLLKIIAEKYKKQITLEAYDDFVLDRSLNGSRFREATGYMPPQ
                     WPELIDRMHRDYVSHQAWYQKSH"
     gene            1976..3109
                     /gene="wecB"
                     /locus_tag="PK590_06195"
     CDS             1976..3109
                     /gene="wecB"
                     /locus_tag="PK590_06195"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOG24220.1"
                     /translation="MKLKVMTILGTRPEIIKLSRIIHQLDKDFHHILVHTGQNYAHEL
                     NEIFVQEMQIKKPDYNLSVGSDRLADTIGKIISESDKIMEAENPDALFVLGDTNSCLS
                     VIPAKRRKIPIFHMEAGNRCFDARVPEEINRRIVDHTSDINLTYSEFARGNLLREGIP
                     ADRIIKVGSPMREVLNFYADKIDQSSILETLNLNAENFFLVSCHREENVDSPTQLKKF
                     IEVLNKIRREFNLPVLVTTHPRTRKRIEQQNFKLDPSIQLLKPFGFFDYVKLQQSAKV
                     TLSDSGTITEEASLLNFPALNLRESFERQEGMDEAAVMLTGLDCSRVIQGIKNLESRS
                     RGTQRELQIVGDYLPDNVSIKISRIIFSYINFINEKVWMKNRS"
     gene            3402..3629
                     /locus_tag="PK590_06200"
     CDS             3402..3629
                     /locus_tag="PK590_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24221.1"
                     /translation="MNASFKLLKMLGIAITIPTMLISGPLAGFLIATWLINKWNFSPK
                     WIMICVLLGLLGSSIQITRLIKHLYKESRSG"
     gene            3632..>3903
                     /locus_tag="PK590_06205"
     CDS             3632..>3903
                     /locus_tag="PK590_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24222.1"
                     /translation="MEEHTVASLAQTAHAVAQSHGEAHELPNFLSILAQLFPDCAIFN
                     FFHRWENVVFSVLVAGFISWRAIVAARSGQFIPKGAQNVWETVTEGL"
BASE COUNT         1096 a          948 c          884 g          975 t
ORIGIN      
        1 gattatactt cggataatac cgatcgattg aacccctcag aaattgaaac cctgctgttg
       61 ggcctgtccg aagtaaaaaa ggagcttgag gacagatgaa ggctttagtc acagggggag
      121 ccggctttat cggctcaaac ctggttcgat tgctggttga aaaaaaacat agtgtcaccg
      181 tgctggacaa tttgtcaaca ggacatctga gcaacctggg gccttttccc agcgtcaaat
      241 tcattaaggg agatattcgt gacgaaagaa cgatttcaag ggccttgcgg ggcgttgaca
      301 ttgtttttca tctggccgcg tcggtcggca attccctttc catcaaagcg cccgtatacg
      361 attcagaaac aaatgtttta ggaaccctca aattgttgcg ggccgctcac caaaaccgca
      421 taaaaaaaat agtctattcc tcatcagccg gtattttggg ggaactcaaa gaactgccca
      481 tcaacgaaaa ccacccgacc aatcccattt cgccgtatgg cgtcagtaaa ttggcggctg
      541 aaaaaatgtg cctggtttac aatagtttat acggcatgaa aaacgtctgt ttgagatact
      601 ttaatgtata tggaataaac cagcggtatg acgcttacgg aaacgtcatt ccgatttttg
      661 cccggcgaat tatccgaaaa caaccactct ttgtttttgg agacggatat caaacccggg
      721 atttcgttaa tgtcattgat gtggcaaaag ccaactatca agccgccatc accggaaaag
      781 tcgcgggagc ttttaacatc gcgagcggcg cctccattac tatcaacaaa ctcgcaaata
      841 tgatgatgca aaccgctcgc acgaccgtcc ccctccagca caaaaaaccg cgcctggggg
      901 atgttcggga tagtctggca aacatttcag ccgcgcggaa agcttttggt ttcaaacctt
      961 ccgtcagtct ggaagaaggt ttaaaaaaat acatgtcctg ggcaagccaa aacagatttt
     1021 gaggagatca accgcggaga tccatagaat gaaaaagaaa atattgattt tgggtgcaac
     1081 cggaatgctg ggacacgttt taatgcggca tctgtcttcc ctgcccgggt atgaggttta
     1141 tggaacggcc agaaatcccg caatcgtcac caggtttttt cctcccgatc tagctgacca
     1201 tttccggcac gacacggtag atgccattaa tattgactcg gtcatccgag cccttgcggc
     1261 ggtagaaccc gatattgtca tcaactgcat cggcctcatt aaacagcttc cgatcgccaa
     1321 cgatcccctc tccgccatta ccattaatgc acagcttccc catcgaattt ccctgatcag
     1381 caaaaccgcg ggggcgcgaa tgattcacat cagtacggat tgcgttttta acggacagaa
     1441 aggtcattac accgaaaacg atccctcaaa tgccgaagac ctttacggaa gaaccaagta
     1501 cctgggagaa gtaagttacc cgcattgcat caccctgcga acttccatca tcgggcacga
     1561 gttggaaaag ggctacggcc tggtcgattg gtttttggcc cagcatggaa cggtccgggg
     1621 atttaccaaa gccatttatt caggattccc aacgattgaa ctggcgcgaa ttttgtctga
     1681 ttacatcttg cctgatgaaa aactcagcgg tgtttatcat gtttcctcag accccatctc
     1741 caaatttgat cttcttaaaa taattgccga aaaatataaa aagcaaatca ccctggaagc
     1801 ttatgatgat tttgtccttg accgttcact gaacggatca aggttccgtg aggccacggg
     1861 gtatatgccg ccccagtggc cggaactcat tgaccgtatg catcgtgact atgtatccca
     1921 tcaagcctgg taccaaaaat ctcactaaaa gtagcgaaag cggaggaaac aagggatgaa
     1981 gttaaaagtg atgaccatct taggaacccg cccggaaatc atcaagcttt ctcggatcat
     2041 ccaccagttg gataaggatt ttcaccacat tctggtccat acgggacaaa actacgctca
     2101 tgaattaaac gaaatctttg tccaggaaat gcaaataaaa aagcccgatt acaatctttc
     2161 ggtcggcagt gaccggctcg cggataccat cggtaaaatt atcagtgaaa gcgacaagat
     2221 catggaagcc gaaaacccgg acgcgctttt tgtcttgggc gataccaaca gctgtttgtc
     2281 cgtcattccg gccaaacggc ggaaaattcc gatttttcac atggaagctg gtaaccgttg
     2341 ttttgatgcc cgtgtccccg aagagatcaa ccgaaggatc gtggatcata ccagcgatat
     2401 taatttgact tacagcgaat ttgctcgggg aaatttgctg cgggaaggga ttccagcgga
     2461 ccggattatc aaggtgggta gccccatgag agaggtcttg aatttttacg ctgacaaaat
     2521 agatcaatcg tcgattcttg aaacactgaa tttaaatgct gaaaatttct tcctagtcag
     2581 ctgtcatcgt gaagaaaacg ttgattcacc aacccagctg aaaaaattca ttgaagtgct
     2641 caataaaatc cgtcgtgaat tcaatttgcc cgtactcgtt actactcacc ccaggacccg
     2701 gaaacgaatt gaacaacaaa acttcaagct ggatccctcc attcaactcc ttaaaccttt
     2761 cggttttttt gattatgtga aactacaaca gtccgccaag gtcactttgt ccgacagcgg
     2821 cacaatcacg gaagaggctt ccctcctcaa cttcccggcc ctcaacctca gagaatcttt
     2881 tgagaggcag gaaggaatgg atgaggcggc cgttatgctg accggattgg attgttccag
     2941 ggtgattcag gggattaaaa atttggaaag ccggtctcgc gggacccaga gggaacttca
     3001 gatcgttggc gactatcttc ccgacaatgt ctcaatcaaa atttccagga ttattttcag
     3061 ctatatcaac ttcatcaacg aaaaagtctg gatgaaaaac cgctcctgat tttagtctga
     3121 taaatttcgg cgccagccag actcgggtag gaaagagctg gctattttga atttttacgt
     3181 tccaccaaag acaagaatgg cagggcgatt tcgcaattac tttcttacac ttgcaaagaa
     3241 acttataaag ccaatccaag tatccaaatc tgttgcggaa aacagtccgc gtcccgcatg
     3301 gcgggaatgt cagtcgatat ccggaactga gagctaacgg tcttaaacac accttcggct
     3361 caatgcggca aaatcatcta ctaccaaaca aaggccacac gatgaatgct tcttttaaac
     3421 ttctcaaaat gctgggaatt gcgatcacca tccccacaat gttgatttcg ggaccgcttg
     3481 ctggcttttt gatcgcgacc tggctcatca ataagtggaa ttttagtccg aagtggatta
     3541 tgatatgcgt gcttttgggt cttttaggtt cctcaatcca aatcacacgg cttattaaac
     3601 acctctacaa ggaaagtcga tcaggctgac gatggaagaa cacacagtgg cttcgctcgc
     3661 acaaaccgca cacgcggtcg ctcagtcgca cggagaagct cacgaactcc ccaattttct
     3721 gagcatcctg gctcaacttt tcccggattg cgcgatcttt aatttttttc atcggtggga
     3781 aaacgttgtt ttttcggttt tggtggcggg ttttatcagc tggagggcta tcgtcgcggc
     3841 cagatccgga caattcatcc ccaaaggcgc gcaaaacgtt tgggagaccg ttacggaagg
     3901 gct
//
LOCUS       DAOSIO010000160         3900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_20501_length_3900_cov_3.663199, whole genome shotgun sequence.
ACCESSION   DAOSIO010000160 DAOSIO010000000
VERSION     DAOSIO010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20501_length_3900_cov_3.663199"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..1395
                     /locus_tag="PK590_06210"
     CDS             136..1395
                     /locus_tag="PK590_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HOG24223.1"
                     /translation="MQFFKWFYPGIGIKRWIIVCVIGLALLVMVALLTVKTIAKTSAV
                     QASFTTAALIFGIFLIYTSIKNMVRIFVRALMPPHRDGRLVDIVYQKRQSESLLRGPR
                     VVAIGGGTGLSTMLEGIKHFTSNITAIVTVTDTGGSSGRLRDEMDMLPPGDIRNCLVA
                     LADAGPLIRDLFQYRFVTGEGLKGHSFGNLFITALSMVTGDFEKAIAESSKVLAIRGR
                     VLPSTLDKVTLVGEFTDGTRQEGETNITGARKPLRSIHLKPDDCKACPEALDAISSAD
                     LVIMGPGSLYTSILPNLLIQDIRDSIVNSDAYKVYICNAMTQPGETVGFSAWDHLRTI
                     IEHTDPRIVDAVYVNTAKIPEPLFQRYREVGALPVKLDLDKILDKGYELVTDEVLKSD
                     SQVRHDAEKIMKSIIDHYMKNEKKLRG"
     gene            1392..1703
                     /locus_tag="PK590_06215"
     CDS             1392..1703
                     /locus_tag="PK590_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOG24224.1"
                     /translation="MKMQGRKPLKGRKEMGRVEKDLVITNKLGLHARPAALFVQTANR
                     FDATIEIQKDDMSVDGKSIMGIMTLAAEVGSTIKVIASGKDASAAVDALMKLVETNFG
                     E"
     gene            1707..3455
                     /gene="ptsP"
                     /locus_tag="PK590_06220"
     CDS             1707..3455
                     /gene="ptsP"
                     /locus_tag="PK590_06220"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOG24225.1"
                     /translation="MREFKGVGVSPGIAIAKVFVLHSGGSFNLPKREITPDQIPGEIT
                     RFEDALARTRTEILEIRRKLSDQIGRESSDIFTAHLLILEDRTLIEDVIEVIRNEQGV
                     AEYAFATVMQRYFQAFSQINDEYLKERISDIRDVGRRLLGNLCGEEKRRLEELPEKVI
                     IISHDLSPSDTATLDKSKVLAFVTEIGGPTSHTAILGRSFEIPAVVGTGKIISEINNG
                     MTVIVDGTHGVVVVDPDQATIDKFTSEGDRFVQISGELEKLRDLPTETLDGKRVVLGA
                     NIEFHHEVASVISHGAEGVGLYRTEYLYMNRIALPTEEEQYEAYRKVAETMNPRAVII
                     RTLDLGGDKFASTLEIPHEMNPYLGWRAIRFCLTRKDIFKTQMRAILRASAHGNVKIM
                     YPMISNIYEIREARELLNLSMAELEREGKPFNRQIDVGAMIEIPSAALTSDVLAQEVS
                     FFSLGTNDLIQYVLAIDRTNEKIAYLYEPTHPAILRLVEMTINNGHRGKIWVGSCGEM
                     SANPKIAILLLGLGIDEISASPIVLPKIKKAIRSIKYSDAREIAQKALSLPTGKEIDQ
                     FLNEKLKDICKDLVDE"
     gene            3464..>3900
                     /locus_tag="PK590_06225"
     CDS             3464..>3900
                     /locus_tag="PK590_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HOG24226.1"
                     /translation="MEIYLKDLSNQIKKAQSIASGVKLPGTFVKRPAKVLFCGMGGSA
                     ISGDILGTIAQSRSRIHWTVNRTARLPQWVDSKTIVILSSYSGNTHEVESIFQQAVAR
                     KAHFLLVASGGRLAEEAFRRKIPFLRLPQGYPPRFAIGYGTFAL"
BASE COUNT          933 a          900 c         1109 g          958 t
ORIGIN      
        1 gcgcagattt cgagattatt gcgggattct tacttttgtg agcttctccg ccgggccgcg
       61 acccccaaag atgttttcga gctgattagc aaagaagaaa atcgcaaagg ctaagatctg
      121 gtttagggtc cacgaatgca gttcttcaag tggttctatc ccggcattgg catcaaacgc
      181 tggattatcg tatgtgtgat cggtctcgcc ctgcttgtga tggtggcgct tctgacggtg
      241 aagacgatcg caaagacgag cgcggttcag gcttcgttta ccacggcggc gctgattttt
      301 ggaatttttc tcatctatac atcgatcaaa aacatggtgc gtatttttgt ccgtgccctt
      361 atgccgcctc atcgcgatgg ccggcttgtg gatattgttt accagaagcg tcagtcagag
      421 tcgcttttgc gcggaccccg ggtggtcgcg atcggaggtg gtacgggact cagtaccatg
      481 ctggaaggca tcaagcattt taccagtaac atcacggcga tcgtgacggt gacggacacg
      541 ggcggaagtt cgggccggct tcgtgacgaa atggatatgt tgcctccagg tgatatccgg
      601 aactgtcttg tggcgcttgc cgacgccggc cccctgattc gggatttgtt ccagtaccgt
      661 tttgtgacgg gtgaagggct taaggggcac agtttcggca acctgttcat cacggccctt
      721 tcgatggtca ccggggattt tgagaaagct atcgctgagt ccagtaaagt gctcgcgatt
      781 cgcggacgtg tcctgccttc cactcttgat aaggtgactt tggtggggga gtttacggat
      841 gggactcgcc aggaagggga aacgaatatt acgggcgctc gcaagccgct gaggagtatt
      901 catttgaaac cggatgattg caaggcgtgt ccggaggctt tggacgcgat atcgtcagct
      961 gatctcgtca ttatggggcc cgggagtctt tatacgagta ttttgcctaa ccttttaatt
     1021 caagacatcc gggactccat tgtgaattcg gatgcctaca aagtttacat ctgtaacgcc
     1081 atgacccagc cgggcgagac cgtgggtttc agcgcgtggg accatttgcg aacgatcatc
     1141 gaacatactg acccgcgcat tgttgacgcg gtttatgtca atacggcaaa aattcccgag
     1201 ccgctttttc agcgttaccg tgaggttggc gcgcttccgg tgaagcttga tcttgataaa
     1261 atcctggaca aaggttatga gctggtgacg gatgaagtcc tgaaatccga tagccaggtg
     1321 cgacacgacg ccgagaagat tatgaaatcg attatcgatc attacatgaa aaatgagaaa
     1381 aagttaagag gatgaaaatg cagggacgga agcccttaaa ggggcggaaa gaaatggggc
     1441 gggttgaaaa agatcttgtg atcaccaaca agcttggtct gcacgctcgt cccgcggcgt
     1501 tattcgtcca aaccgcgaat cgatttgatg cgacgattga aattcaaaaa gatgacatga
     1561 gtgtggacgg taaatccatt atgggcatta tgaccttggc ggcggaggtg ggctcgacga
     1621 tcaaggtgat cgcgtccggc aaagacgcgt cggcggccgt ggacgcgctg atgaaacttg
     1681 tcgaaaccaa ttttggagag taaacgatga gagagtttaa aggcgttggc gtatctcccg
     1741 ggatcgcgat cgctaaagtg tttgttcttc acagcggggg atcatttaat cttccgaagc
     1801 gcgagattac gccggaccag atccctggtg agatcacgcg ttttgaggat gcgctggctc
     1861 gaacacggac cgagatactt gaaattcgcc gcaaactttc ggaccagatt gggcgcgaaa
     1921 gttcggacat ctttacggcg catctgttga ttctggaaga ccggactctc attgaggacg
     1981 tgattgaggt catcaggaac gagcaaggtg tcgcggaata cgcttttgcc acggtgatgc
     2041 aacggtattt tcaggcattt tcccagatta acgacgagta tcttaaagag cgcatttcgg
     2101 acatccggga cgttggccga aggcttctgg gcaatctttg cggtgaggaa aaacggcggc
     2161 ttgaggagct tcccgaaaaa gtcattatca tttctcacga tctttcgcct tccgacaccg
     2221 cgactctcga taaatccaag gtgttggcgt tcgtgacgga aattggcggg ccgacttccc
     2281 ataccgcaat tcttggccga tcatttgaaa ttcccgcggt ggtcgggacg ggaaaaatca
     2341 tcagtgaaat taacaacggg atgaccgtga ttgtggacgg cacccacgga gtcgtggtag
     2401 tcgatccgga tcaggccacg atagacaagt ttacatccga gggtgaccgg tttgtccaga
     2461 tttccggtga gcttgaaaag ctcagggatc ttccaactga gacgttggac ggaaagcggg
     2521 ttgtgcttgg ggccaatatt gagtttcacc atgaagtggc ttccgtcatc tcgcatggcg
     2581 cggaaggggt ggggctttac cggacggaat atctttacat gaaccggatc gcgctcccga
     2641 ccgaagaaga acagtatgag gcttaccgga aagtggcgga gacgatgaat ccccgcgccg
     2701 tgattattcg cacgctggat ttgggcggcg acaaatttgc ttcgaccctt gaaatccccc
     2761 atgaaatgaa tccctatctc gggtggcgcg cgatacgttt ctgtctgacg cggaaggata
     2821 tttttaagac ccagatgcgg gccattcttc gcgcgagcgc tcacgggaac gtcaagatca
     2881 tgtatccgat gatttcgaat atttatgaaa ttcgggaggc cagagagctt ttgaatctat
     2941 cgatggcgga actggaacgg gaagggaagc cgtttaacag acagattgac gttggggcca
     3001 tgattgaaat tccgtccgcg gctctcacaa gtgacgtcct tgctcaagag gttagttttt
     3061 tctccctcgg gacaaatgac ctgatccaat atgtcctcgc gatcgaccgc acgaacgaaa
     3121 aaattgctta tttgtatgag ccgacacacc cggctattct gaggcttgtt gaaatgacca
     3181 ttaacaatgg tcatcgtggc aaaatctggg tcggaagttg cggagagatg tccgcgaacc
     3241 ccaaaatcgc gatccttctt cttgggctcg gcattgatga aattagcgca agtcccattg
     3301 tgttacccaa gatcaaaaaa gccattcgct cgatcaaata ttctgacgcc cgggagatcg
     3361 ctcaaaaagc gctcagtctg ccgaccggga aagaaatcga tcagtttctc aatgaaaagc
     3421 tcaaggatat ttgcaaggat ctggtggatg aataattaaa agcatggaaa tttatcttaa
     3481 agatctttct aatcaaatca agaaggccca aagtattgcg tccggggtca aactacctgg
     3541 caccttcgtc aagcgtccgg ccaaagtgct tttttgcggg atgggtggct cggcgatcag
     3601 cggtgatatt ctgggtacga ttgcgcagag ccggtcgcga atccactgga cggtgaaccg
     3661 gacggctcgt cttcctcaat gggttgactc taaaacgatc gtcatcctct cgagctattc
     3721 ggggaatacc catgaagttg agtctatttt tcaacaggct gtcgcgagga aggctcattt
     3781 tctcctggtg gcttcggggg ggcggttggc ggaagaagcg tttcggcgaa aaatcccctt
     3841 tctcagactt ccccagggat atcctccgag atttgcgatt gggtacggaa cgttcgcgct
//
LOCUS       DAOSIO010000161         3887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_20606_length_3887_cov_3.573330, whole genome shotgun sequence.
ACCESSION   DAOSIO010000161 DAOSIO010000000
VERSION     DAOSIO010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20606_length_3887_cov_3.573330"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="PK590_06230"
     CDS             complement(<1..998)
                     /locus_tag="PK590_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOG24227.1"
                     /translation="MFSLLTAEEQALVERYSRLVGFKRGDVVYEEGTPPEAFYLIISG
                     RFRLFSRAHANEKENTILFFYRGEHFGETSLLTGSVHSATVEAKTDAVVLKIVADDFQ
                     KLLREIPSFALHLSRSLGRHLTRGELGYSRHRREVRIASVYCSEMSRAMTRFLVDFVS
                     ALHSVTQRRILMLDLSGQLYTQLPQDLMGLKLNRLDLATVKITSEGDATETIAKSPHG
                     FDLLSLSQNSSLISEDQRFASLLTCLSYQYDYLVLCLPSRETPVAIKALNYSDRIYLW
                     AENRAEAFSFVAQKTAELCRDFGFNKNEMRIIMPHGERLPELDIVDEVVPVEIQMFS"
     gene            complement(1229..1618)
                     /locus_tag="PK590_06235"
     CDS             complement(1229..1618)
                     /locus_tag="PK590_06235"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOG24228.1"
                     /translation="MNNDKRKFKRFDAYMSIKYSGLDNTTLKGIGLSRDLCREGIKIN
                     SDKEISEGTQLDLEISIPDDPKPIFSSGTVVWTRPSDGSNQGYDQGIRLKNMDPVDKF
                     RVLDYAYNHWLETKVNDLADPESLHEW"
     gene            complement(1868..2371)
                     /locus_tag="PK590_06240"
     CDS             complement(1868..2371)
                     /locus_tag="PK590_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HOG24229.1"
                     /translation="MAQDFSFDIVSRLDLQEVDNAVNQAKKELLTRFDFKGSKSSIEL
                     IREKELVKIRLIADDEMKLRNLQDILKTRAAGRKISIKALDFRTPEKAFDDCLRQDVH
                     LQQGIAHEKAKEIARNIKDQKLKVQTSIQGDQLRVSSKSKDDLQAVIRFVQTLPLDIP
                     VQFVNFR"
     gene            complement(2379..2732)
                     /locus_tag="PK590_06245"
     CDS             complement(2379..2732)
                     /locus_tag="PK590_06245"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOG24230.1"
                     /translation="MWDGFNKRKFPRLHLTCEVVVYPQGRERAIKTITENVGMGGLCL
                     MLDEPLERFDRCKVTLELKDGGPPVQCTGRSVWVVPNRELKSPVKRYDTGIEFLDIDD
                     SSRQRLRAFLSQPQK"
     gene            complement(2735..3751)
                     /locus_tag="PK590_06250"
     CDS             complement(2735..3751)
                     /locus_tag="PK590_06250"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOG24231.1"
                     /translation="MGIFSRKKDEKNSRTSSLTESEIKQKLYGEFNVESSRTAGRRES
                     TFLGKSSGMTAKSSAAQPAVDLFDRPVEQTDFPVTSREIKIPVSGDKQQPFVRPPEKN
                     QTTGRLNGKSSYDQYREYIRRDSNPSFFSSMARFFTDMMGGTGNSNRFLTRQVVYWAA
                     GVLVVFFLFWGVNALNVQREQAMNTKYSVPVEKIQKLPEPAPKVRVTGGVLTVPAADP
                     VPEKNLTLSPAPVPLKPTNVEPAGSVSSVHVSSSRGFAVQVVTYPSEEYASKVVTSLR
                     EAGFKAFVRENVRPTGSVFYIVYIGSFPTAAVARQELARFRAHEISRPFSDAFVKTIE
                     MKES"
BASE COUNT         1057 a          993 c          870 g          967 t
ORIGIN      
        1 gaaaacatct ggatttccac cggcacaacc tcatccacaa tgtccagctc tggcaacctt
       61 tctccgtgcg gcatgataat tcgcatttca tttttgttga atccgaaatc acgacaaagc
      121 tctgcggttt tctgcgcaac aaaagagaat gcctcggccc tattctcggc ccacaaataa
      181 atccggtctg aataattgag cgccttgatg gcgaccgggg tctcacggga aggaaggcac
      241 aaaacaagat agtcatactg ataactcagg caagtcagca aagaagcaaa gcgttgatct
      301 tcagagatca atgaactgtt ttgggaaagt gacaagaggt caaaaccgtg cggacttttg
      361 gcaatcgttt cggtcgcatc gccctcgctt gtgattttta cggtcgcgag atcaagccgg
      421 ttcagtttta aacccatcag atcctgcggc aattgcgtat aaagctgacc ggaaagatcc
      481 agcattaaaa tgcgccgttg tgtcacgctg tgaagagcac tgacaaaatc cacgagaaaa
      541 cgcgtcatcg cgcgtgacat ttcagaacaa taaaccgatg caatgcggac ctcgcgccga
      601 tgacgactat agcccaattc tcctcgagta agatgacggc caagtgaccg gctcagatgc
      661 aacgcgaaag aaggaatttc acgtaacaac ttctgaaagt catcggcaac gatcttgagt
      721 accacagcat ccgttttcgc ttctaccgtg gcactgtgca cgctccctgt caaaagcgat
      781 gtttctccga agtgctcgcc ccggtaaaaa aagaggattg tgttttcttt ttcgttggcg
      841 tgagcgcggg aaaacaaacg aaagcggccg gaaataataa gataaaaggc ctcgggaggg
      901 gtcccttctt cgtaaacaac gtcccccctc ttgaaaccga caaggcggga ataccgctcg
      961 acaagggctt gctcttcggc ggtgagcaaa gaaaacaaag ggatggaatc taaaaaataa
     1021 ccctctttat cactggacct tacctttttg ccgaagggga aaaatttcgc catgccaata
     1081 gtatcggcag accagggaaa tcaccttact gaggtttcag ccctttctgt aagagcttcc
     1141 ttgaagagtg cgacggacgc atcatttcag gcttcaggat gaaaaacaag actcaggaca
     1201 agcacaagaa acagctcatc caacctgctt accactcatg caaactctca ggatccgcaa
     1261 gatcattcac tttggtctct agccagtgat tgtaggcgta atcaagaacc cgaaatttat
     1321 caaccggatc catgttcttg aggcgtattc cctgatcgta gccctgattt gatccgtctg
     1381 agggacgtgt ccacacaacg gtgcccgagc tgaaaatcgg tttaggatca tccggaatag
     1441 aaatttcaag atccaactgt gttccttcac taatttcctt gtccgaattg atcttaatcc
     1501 cctcacggca caggtcgcgg ctcaacccga ttcctttcag ggtcgtattg tccaatcccg
     1561 agtatttaat gctcatgtat gcatcaaagc gcttaaactt gcgcttatca ttgttcatgc
     1621 ggcctccttc gccctcttaa gacttgagaa atatgttcgt tttcttggat agaaactttt
     1681 aaaagagact ttttaatatc gcgatttatc tctaaacacc cctcgcttgc gaaaaaagta
     1741 gcacatggtc accgaaaata caagggcagt acaataatta attcggcctt acgcgggaaa
     1801 gctttagcct acccccttcg caagctctgg aatcaatata acctgttggt atgccagggg
     1861 ttaaatacta tcggaaatta acaaactgaa caggtatgtc gagcgggagg gtctgaacga
     1921 atcggatcac agcctggaga tcatctttgc ttttggagga aacccgaagt tgatcaccct
     1981 gaatcgacgt ctgaaccttc agcttctggt ctttaatatt tctcgctatt tctttggcct
     2041 tttcatgggc aatgccctgc tgaagatgca catcctgacg gagacaatca tcaaatgctt
     2101 tttccggagt cctgaaatca agggctttta tggagatttt tcgccccgcc gcgcgcgtct
     2161 ttaaaatatc ctgaagattg cgaagtttca tttcgtcatc cgcaatcaat cgtatcttca
     2221 ccaattcctt ttcgcggatt aattcgattg agctcttgct acctttaaaa tcgaaccggg
     2281 tcagcaattc ctttttggcc tgattgacgg cattgtcaac ctcttgcaag tccagcctcg
     2341 aaacaatgtc aaaagaaaaa tcttgcgcca taaaataact atttctgagg ttgggataaa
     2401 aaggcgcgta atcgttggcg ggaagaatca tcaatatcaa gaaactcaat accggtatcg
     2461 tagcgtttta ccggagattt gagctcgcgg ttaggaacaa cccaaacact ccggcccgtg
     2521 cattgcacgg ggggtccccc atccttaagc tcaagcgtaa ccttgcatcg atcaaaccgc
     2581 tccaagggct catccaacat aagacacagc cctcccattc cgacattttc cgtaatggtc
     2641 ttgatggcgc gctcgcgacc ttgaggataa acaaccactt cgcaggtcag atggaggcgg
     2701 ggaaatttcc gtttattaaa accatcccac acgattaact ctccttcatt tcaatagttt
     2761 tgacaaacgc atcggaaaat ggccgggaaa tttcatgcgc tcgaaacctg gcaagttcct
     2821 gccgggcgac ggccgcagtg ggaaaactgc cgatataaac aatataaaaa acggatcctg
     2881 ttggccgaac attttctcgc acgaaggcct tgaaaccggc ttcccgcaaa ctggttacaa
     2941 ccttgctggc atattcctcg gaaggatacg tcaccacttg cacggcaaat ccacgagaag
     3001 acgaaacgtg gactgacgaa accgatccgg caggttcaac gttggtcggc ttcaagggta
     3061 cgggagccgg gctcagtgtc agattttttt cggggactgg atccgccgca ggaactgtca
     3121 atacaccacc cgtcacacga actttggggg caggctcagg taatttctga attttttcga
     3181 ccggcaccga atacttcgta ttcatagctt gctcccgctg aacattgaga gcattcacac
     3241 cccaaaaaag gaagaacacg accagaactc ccgcggccca gtaaaccacc tgtcgggtca
     3301 aaaacctgtt cgaatttccg gtacccccca tcatgtcagt aaaaaaacga gccatcgatg
     3361 aaaaaaacga cggatttgaa tccctcctta tatactcgcg atactgatca tacgaacttt
     3421 ttccgttcag cctcccggtc gtttggttct tttcaggagg acgaacaaac ggctgttgtt
     3481 tatcgcctga aaccggaatt ttgatctccc ggctggtaac cggaaaatca gtctgctcaa
     3541 caggcctgtc aaacaaatcc acggctggct gagccgccga agatttcgcg gtcatcccgg
     3601 aggacttccc gagaaacgtc gactcccgcc tccctgcggt ccgggaggat tcgacattga
     3661 actctccata aagcttttgt ttgatttcag attccgtcag cgaggaggta cggctatttt
     3721 tttcgtcttt ttttcggcta aagataccca tgcgaatggt tacccttttt gcggatttaa
     3781 attgagagag aattttaaaa gttcaatggg acctaacgcg atgtgcgccc gttgcacaaa
     3841 accgtttttc atgcaaagct ggatcgcgcc cgacatcctg gaagttg
//
LOCUS       DAOSIO010000162         3885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_20614_length_3885_cov_4.232637, whole genome shotgun sequence.
ACCESSION   DAOSIO010000162 DAOSIO010000000
VERSION     DAOSIO010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20614_length_3885_cov_4.232637"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PK590_06255"
     CDS             <1..760
                     /locus_tag="PK590_06255"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG24232.1"
                     /translation="IFFDGCSSRCFFCQNYQISAAHHGEIYSADRFFEAVKDLIDQKV
                     HNLNFVTPDHFWPHIKELCLKLRALSFPIPKVFNSSGYQLPEMISEYSEFMDIFMPDF
                     KFADPDLARECMGDERYPEIALEAIREMVDARGFLDPWDPSGERVAERGVLVRHLVLP
                     GHVENSLETLNQLHAEFGDGLPISVMSQFRPVPACFAKNSLARGVTPQEYNLVLAKVK
                     ELGFEKVYTQILSDQTDYLPDFNNSEDPFPGHRK"
     gene            1080..2789
                     /locus_tag="PK590_06260"
     CDS             1080..2789
                     /locus_tag="PK590_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24233.1"
                     /translation="MKINYALRVAVASMFIIQQLFSVPASGLAAHVPADDQPVKAYQS
                     EVGFDQSTGQDANASSPKATTTDFLSGETGVLTRTQGDSSKAELESYLQAVTDAGREY
                     LEAIRDDFEQIAGVSGMVSRDELERLISDMGLNEAEASRLRNLHTYCRLNSTSASSIL
                     SGDYGLMHREFPEFFNAEGNPTEALNKFREWATSERLPLTSPVMVFMPPTGSSIGELP
                     IETLRKLADAGRAVLGDDPKAYYARAQAEIEQNVPKIVQAMEKIKKEIVELKKRVPLA
                     VQSFVKELQKQIGDGFRVEVRKETNGTYRVYVSALRMTSKPPLKGTLTAMIARLDGTG
                     KVLSVVANYSGISVDTKLLFEAMGVCPVGQKCAAMMDVEKLAQMIKITVTKVASRVIY
                     YSEDNVDHMTYRDSKGRVIVEQLSPPATVQRFVNELQKQIGDGFRVEVRKETNGTYRV
                     YVSALRMTSKPPLKGTLTAMIARLDGTGKVLSVVANYSGISVDTKLLFEAMGVCPANQ
                     NCVSIAMTNVEKLSRMAKITVVKTTSRAIYYLEDKVEYLAMWKDSQRRRVRLRAGWVL
                     SGK"
     gene            3097..3780
                     /locus_tag="PK590_06265"
     CDS             3097..3780
                     /locus_tag="PK590_06265"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOG24234.1"
                     /translation="MSLTIKNFQVSPSGLSLFKDCPRCFWLEKVRGIKRPRGIFPSLP
                     SGMDRVIKVYFDKFRARGALPPELSARPEFRGLRLYENHAQLERWRNWRTGLEFFENG
                     ARLFGALDDLLVASDLYVPFDYKTKGSPTSEADATKYYQTQLDCYALLLEANGMKTAG
                     YGFLLYYSPSEVNESGNVAFEIQPIKIETDIERAKSLVRQSAAVTGGAAPKASANCEY
                     CNWLEKFRK"
BASE COUNT          987 a          807 c         1093 g          998 t
ORIGIN      
        1 tatctttttt gacgggtgtt caagccgatg ttttttttgc caaaactacc agatttccgc
       61 ggcccatcat ggcgagattt actcagcgga ccggtttttt gaagccgtca aggacttgat
      121 agaccaaaag gttcacaacc tgaactttgt cactccagat catttttggc cgcatatcaa
      181 ggaactctgc ctaaaacttc gggctctgag tttcccaatc ccaaaagttt tcaacagctc
      241 gggatatcag cttccggaaa tgatctccga atatagcgag tttatggaca tcttcatgcc
      301 ggattttaaa tttgcggatc ctgatctcgc ccgtgagtgc atgggggatg agcgttatcc
      361 agagattgcg ctcgaagcga tccgggagat ggtcgatgcg aggggatttc ttgatccctg
      421 ggatccctcg ggggagcggg tggcggagcg gggcgttctg gttcgtcatc ttgtgttacc
      481 gggccatgtc gaaaacagcc ttgaaacgct taaccagctg cacgctgagt ttggcgacgg
      541 gctccccatt tcagttatga gtcagtttag acccgttccc gcctgcttcg cgaagaattc
      601 actcgcgcgc ggagtcacgc cgcaagaata caatcttgtc ctcgcgaagg tgaaagagct
      661 tggatttgaa aaagtctata cgcagattct gagcgatcaa accgattacc tgcctgactt
      721 caataactcc gaggacccgt ttccgggaca tcggaagtaa tctcccaaga aatccgctaa
      781 ccattgacct agcctgaaag cgtagtattg ctataaattg ttatattttc taataattaa
      841 agagtatcta tcgctaatat ttttcagaat taaattaaaa caacttgtta ccatgagccg
      901 atcgtaaaaa tatagtcaat tatagcgatg tatagcatta atgcttttat tgtgttggtg
      961 catttgacaa acagattttg tgatgtttaa ctcattatat gaacatatta aacaggatta
     1021 ttaagttgtt agttacggaa aaacaaatca aaagaaaatt cacacggagg tttcccacca
     1081 tgaagatcaa ttacgctttg agagtcgcgg ttgcgagcat gtttatcatc cagcaattat
     1141 tttcagttcc cgcgtcgggt ttggccgccc acgttcctgc tgatgatcaa ccggtcaagg
     1201 catatcagtc cgaagtcggt ttcgatcagt cgacaggaca ggatgcgaat gcttcctcgc
     1261 cgaaagcaac gacgacagat tttttatcgg gggaaacggg tgttctgacg cggactcagg
     1321 gagatagctc taaagccgag ctggagtcat atctccaagc agtcacggat gctgggaggg
     1381 agtatctgga agctatacgc gatgattttg agcagattgc gggtgtttcc ggcatggtct
     1441 ctcgtgatga actggagcgt ttgatcagtg acatgggatt aaatgaagcg gaagcgagcc
     1501 gtcttcgtaa tctgcataca tattgcagat taaatagtac gagtgcatcc tcgattttga
     1561 gtggggatta tggtttgatg caccgcgagt ttcccgagtt ttttaatgcg gaagggaatc
     1621 caacggaagc cctgaataag tttcgtgagt gggcgacaag tgagaggttg ccgttgacat
     1681 cgccggtcat ggtgtttatg ccaccgactg gttctagcat cggggagtta ccgatagaaa
     1741 ctttacggaa acttgcggac gcaggacgag ctgtgctggg cgatgatccg aaggcatact
     1801 acgcgcgagc ccaggcggag attgaacaga atgtcccgaa gattgtgcaa gcgatggaga
     1861 aaatcaaaaa agaaatagtt gaattaaaga agagggttcc tcttgcggtt caaagtttcg
     1921 tgaaggagtt gcagaagcaa attggggatg gttttagggt agaggtgagg aaggagacca
     1981 acggaacgta tcgggtctat gtttctgcct tgaggatgac atcgaagcct cctctaaagg
     2041 gaactttgac cgccatgata gcgcgcttgg atggcacggg gaaagttctt tcggttgtgg
     2101 cgaactatag tgggatcagt gtcgacacga agttactctt tgaagcgatg ggtgtgtgtc
     2161 cggttggcca aaaatgtgcc gcgatgatgg atgttgaaaa gttggcgcag atgataaaga
     2221 ttacggtaac gaaagttgca agcagagtga tttattattc ggaagacaac gtagaccaca
     2281 tgacctatcg tgacagtaaa ggtcgagtga ttgtggagca gttgtcgccc cctgctacgg
     2341 ttcaaaggtt cgtgaatgag ctgcagaagc aaattgggga tggttttagg gtagaggtga
     2401 ggaaggagac caacggaacg tatcgggtct atgtttctgc cttgaggatg acatcgaagc
     2461 ctcctctaaa gggaactttg accgccatga tagcgcgctt ggatggcacg gggaaagttc
     2521 tttcggttgt ggcgaactat agtgggatca gtgtcgacac gaagttactc tttgaagcga
     2581 tgggtgtgtg tcctgctaac cagaattgtg tctcaatcgc gatgacgaat gttgagaagt
     2641 tgtcacggat ggcaaagatt acggtcgtga agactacgag cagagcgatt tattacttgg
     2701 aagacaaggt ggaatatctg gccatgtgga aggactcaca gcgtaggaga gtacggcttc
     2761 gcgcgggttg ggttttgtcc ggtaaataag atgccggacg atactcttca ggtaaaaagc
     2821 gggtgtcgtg ggttgccttg aaatgtaata actagcgggg ctggtttgat agcggttcct
     2881 accaacgcct ccggggcagt aaagccgtaa gagctcattc agacggcatg ccagccccgg
     2941 aggtctcgcc tttgtgatcc ctgagtttca acgcgtattt ctttcgaaag aggagaaaat
     3001 cccgctcgga tttggcgcgc gacgtgatta ttgtgagacg ggatggggtt tctcatcgtg
     3061 cgttaagcga aaaaagttat ataataccca cggcttatga gcttaacgat caaaaacttt
     3121 caagtatcac cctcggggtt gagcctgttt aaagattgcc ctcgttgttt ctggctggag
     3181 aaggttcggg ggatcaagcg gcctcgtggt attttcccgt ctcttccaag cggcatggat
     3241 cgggtgatca aggtttattt tgacaagttc cgggcccggg gcgcgcttcc tcccgaactg
     3301 tcggcaaggc cggaattccg gggacttcgg ctttatgaaa atcacgcgca acttgaacga
     3361 tggcggaact ggagaaccgg actggaattt tttgaaaacg gagcgcggct tttcggcgcc
     3421 ctggacgatc ttctcgtggc cagtgatttg tatgttcctt ttgattacaa aaccaagggg
     3481 tctccgacga gtgaggcgga cgcgacgaaa tattatcaga cccagcttga ttgttatgct
     3541 cttctgcttg aggcgaacgg gatgaaaacg gccggttatg gttttctcct ttattattcc
     3601 ccgagcgaag tgaacgaaag cgggaatgtt gcgtttgaga ttcagccgat caaaattgaa
     3661 actgatattg agcgcgcgaa gtcactggtg cgtcagtcgg cggccgtgac cgggggtgcg
     3721 gctccgaagg catcggccaa ttgcgagtat tgcaattggc tggagaagtt cagaaaatga
     3781 aaaggttcca ggccgacgga acccgggaat aacagacaat gaccggaacc cttcgccctg
     3841 cttctgacgg atccaaagag tctccggggc gtagacagga ttata
//
LOCUS       DAOSIO010000163         3858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_20783_length_3858_cov_4.313963, whole genome shotgun sequence.
ACCESSION   DAOSIO010000163 DAOSIO010000000
VERSION     DAOSIO010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20783_length_3858_cov_4.313963"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..1460
                     /gene="ahcY"
                     /locus_tag="PK590_06270"
     CDS             36..1460
                     /gene="ahcY"
                     /locus_tag="PK590_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOG24235.1"
                     /translation="MKKKKASGKIKDFKVADISLADWGRKEIEMAEKEMPGLMATRRK
                     YGPEKPLKGKRIMGSLHMTIQTAVLIETLVELGADVRWCSCNIFSTQDHAAAAIAKRG
                     IPVFAWKGETLAEYWWCTIQALTFPGGKGPNLIVDDGGDATLMVHLGYEAERNPKLLN
                     KKVETQDEVELYKILKQNLKVNPKKWHGVVEEWEGVSEETTTGVHRLYKMHEEKRLLV
                     PAINVNDSVTKSKFDNLYGCRESLADGIKRATDVMVAGKVVVICGYGDVGKGSAQSMA
                     ALKARVIVTEIDPICALQAAMAGFQVARLEDVLGLADIFVTTTGNVDVITAEHMSKMK
                     DEAIVCNIGHFDNEIQVHQLEKWPRIKKINIKPQVDKYVFPDGHSIILLAQGRLVNLG
                     CATGHPSFVMSNSFTNQTLAQIDLVRNRREPGVYRLEKKLDEEVARLHLDKLGAKLTK
                     LTKKQADYIGVPLEGPYKPEHYKY"
     gene            1642..2784
                     /locus_tag="PK590_06275"
     CDS             1642..2784
                     /locus_tag="PK590_06275"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015878578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOG24236.1"
                     /translation="MDNFTYKNPTEILFGKGMIPELKGRIPASLKVLFLYGGGSIKKN
                     GVYDQVRDVLKGRKVIEFSGVEPNPSYETCLKAVEIVRKEKVGFILAVGGGSVIDAAK
                     FIAAAVLFKGKDPWVILEEGGSNVKMALPIGTILTLPATGSESNGNAVISRGSTHEKL
                     SFYSSEVYPVFSVLDPKTTYSLPAKYVRNGIVDAFVHVMEQYMTYPAKVPLQDRLAES
                     LLQTLIEIAPAAMKRPVQYEARATFMWSATLALNYLISCGAPQDWSAHMIGHELTAFY
                     GLDHAETLAIVLPGVWRHQFKAKQKKLEQYAGRVWGLRTAEEAIVKTEEFFHSLKMPT
                     RLGHYKISAEEAAAKIGARFRDRKVFLGEHGTIGAVAIAEILRSRA"
     gene            2792..>3858
                     /locus_tag="PK590_06280"
     CDS             2792..>3858
                     /locus_tag="PK590_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG24237.1"
                     /translation="MTVLLQVNAVCKAYGASIILENASAAFGDDQKIGVIGRNGAGKS
                     TLCRIITGHETADSGAIIKNSVLKLSYLEQHDSFRPEETVVDFLMRTTGSEHWECAQV
                     SARFNITNEMLEAPVRSLPGGYQTRVKLSAMLLGDPNFLVLDEPSNYLDLNTLILLEN
                     FLLDFRGGFLVVSHDREFLKRTCDHTLEVEGGELTLYPGNIEEYFEFKGVQTAQIAAH
                     NRGVEKKREQLQAFIDRFRAKASTASRAQSKMKQLSKLKTIEIAHPMSHVKIPIPPVE
                     SKAGIAFSCEGLTIGYPGKDVASGIEFEIDRGCRVAVLGDNGEGKTTLLRTMSGDLAP
                     KRGSFRWGTGLKIAFYAQHVFS"
BASE COUNT          958 a          864 c         1104 g          932 t
ORIGIN      
        1 gtcctattaa tcacaacccg accaaaggta aaaaaatgaa aaagaaaaaa gcatcaggga
       61 agataaaaga ttttaaggtg gcggatattt ctctggcgga ttggggtcgt aaggaaattg
      121 aaatggctga gaaggaaatg ccgggtttga tggcgacccg tagaaaatac gggcctgaaa
      181 aaccgctcaa gggaaaacgg attatgggga gtcttcatat gacgatccag accgccgttt
      241 tgattgagac gctcgtggaa ctcggggctg acgtccggtg gtgttcctgt aatatttttt
      301 ctacccagga ccatgcggcc gccgcgatcg caaaacgtgg aattccggtg ttcgcctgga
      361 agggggaaac tctcgcggaa tactggtggt gcacgattca ggcgctaacc tttccgggtg
      421 gaaaagggcc gaacctgatt gtggatgatg ggggcgacgc gaccttgatg gtccacctgg
      481 ggtatgaagc tgagcgaaat ccgaaattgc ttaacaagaa ggtcgagacg caggatgagg
      541 tggagcttta taaaatcctg aagcaaaacc ttaaagttaa tccaaaaaaa tggcacgggg
      601 tggtggagga gtgggaaggt gtctctgagg agacgaccac gggtgttcac cgtctttaca
      661 agatgcacga agaaaaaagg ctccttgttc ccgcgattaa cgtcaatgac tctgtcacaa
      721 aatccaaatt tgacaacctt tacggttgcc gcgaatcctt ggcggatgga attaagcgcg
      781 cgacggacgt gatggtagcc ggaaaagtag tcgtgatctg cgggtatggc gatgtgggaa
      841 aaggaagcgc gcagtccatg gccgcattaa aggcaagggt gatcgtaacg gagattgatc
      901 caatctgcgc cctgcaggcg gcgatggcgg gttttcaggt ggcgcggctt gaagacgttc
      961 tgggcctcgc ggatattttt gtgaccacca cgggtaacgt ggatgttatt acggctgagc
     1021 acatgtccaa aatgaaggac gaggcgatcg tttgcaacat tggccatttt gataacgaaa
     1081 ttcaggttca tcagcttgaa aaatggcccc gaattaaaaa gatcaatatc aagccgcagg
     1141 tcgacaaata tgtgttcccg gacggccact cgatcatttt gctggctcaa ggacgtctcg
     1201 tgaatcttgg gtgcgcgacg gggcatccca gttttgtgat gtcaaattcc ttcacaaacc
     1261 agactttggc gcagattgac cttgtccgta accgccgtga gcccggtgtc tatcgtctgg
     1321 aaaagaaatt ggacgaagag gtggcccggc ttcatctgga caaactcggg gcgaagttga
     1381 caaaattgac caagaagcag gctgattata tcggcgtgcc cctggaaggc ccctacaagc
     1441 ccgagcacta taagtactga tcgagaacgg gttgtcccgg agggggacaa cccgtttttt
     1501 atctgcgttt aaatctagcc gtaaaatttc ccacattata gaacagaacc aaacgggatc
     1561 tctgggttga acctctgccg cctgggatgg ccgtcgcggc cattagctga atcgcgttca
     1621 tattggctga aggagtgatg gatggataat tttacttaca agaaccccac ggaaattctt
     1681 tttgggaaag gcatgattcc ggaactgaaa ggacggattc cggcttctct gaaagttctc
     1741 tttctttatg gtggcggctc cattaaaaaa aatggtgttt atgaccaggt gcgcgatgtc
     1801 ttgaagggac ggaaggttat tgaattttcg ggtgtggagc ccaacccttc ctacgaaact
     1861 tgtctcaagg cggttgaaat tgtcagaaag gaaaaagtcg gttttattct cgccgtgggc
     1921 ggaggctccg tcattgatgc cgcgaaattt atcgcggcgg ccgttctgtt caaggggaaa
     1981 gatccctggg taattctgga agaggggggt tcgaatgtga aaatggcgct cccgatagga
     2041 acgatattga cgcttccggc gaccggatct gaatccaacg gtaatgccgt gatttcaagg
     2101 ggatcgaccc atgagaaact ctcgttctac tcctcggagg tttatccggt tttttcggtt
     2161 ctggatccca aaacaaccta ttcgcttccg gcgaagtatg taaggaacgg aattgtcgat
     2221 gcctttgttc atgtgatgga acagtacatg acctatcctg cgaaggtgcc tctccaggat
     2281 aggctggcgg agtctttact ccaaactctg attgaaatag ctcccgcggc gatgaaaaga
     2341 ccggttcaat atgaggctcg ggctactttt atgtggtcgg cgacccttgc cctgaattat
     2401 ctgattagtt gtggcgcgcc tcaggattgg tccgcccaca tgatcgggca tgaattaacg
     2461 gcattctacg gactggatca cgcggaaacg ttggctatcg tgctccccgg ggtctggagg
     2521 catcaattta aggctaaaca aaagaagctg gagcagtacg ccgggcgcgt gtgggggctt
     2581 cgtactgccg aagaagccat cgtgaaaacc gaggaatttt tccactccct gaagatgccg
     2641 acccgattgg gacattacaa gatttctgct gaagaagcgg ccgcgaagat cggtgcccgg
     2701 ttcagggatc gtaaggtgtt tttgggcgaa cacggtacga tcggcgcggt cgccattgct
     2761 gaaattcttc gttcaagggc atgaccggtt catgacagtt cttctccagg tcaacgcggt
     2821 ctgtaaagcg tacggggcat cgatcattct tgagaatgcc tccgccgcct ttggggatga
     2881 ccaaaagatt ggcgtgattg gcagaaacgg cgccggtaaa tcgacccttt gccgaatcat
     2941 caccgggcat gagacggctg atagcggtgc catcataaaa aattccgtcc tgaaactaag
     3001 ttatctggaa cagcacgatt cttttcgtcc tgaggaaacg gtggttgatt tcctcatgcg
     3061 aaccaccgga agtgaacatt gggaatgcgc tcaggtgtcg gcgcggttta atattaccaa
     3121 cgagatgctg gaagctcccg ttcgcagtct cccgggcggt tatcagaccc gcgtgaagct
     3181 ttccgccatg cttctcgggg atcctaattt tttggttctt gatgaaccca gcaattatct
     3241 cgatttgaac acactgattc ttctcgagaa ttttcttttg gattttcgcg gagggtttct
     3301 ggtggtttcc cacgatcgcg aatttttgaa gcggacgtgt gatcacaccc ttgaagtgga
     3361 gggtggtgag cttacgcttt atccgggtaa cattgaagaa tattttgaat ttaagggagt
     3421 gcagaccgct cagatcgcgg cgcacaacag gggagtggag aagaaacgtg aacagctcca
     3481 ggcttttata gatcgtttcc gcgcgaaagc ttccacggct tcccgagctc agtcgaaaat
     3541 gaaacagctg tctaaactta agacgattga gatcgcgcat cccatgagcc atgtcaaaat
     3601 tcccatcccg cccgttgaat cgaaggccgg cattgccttt tcgtgtgaag gcctgacgat
     3661 cggatatccg ggaaaagatg tcgcgagcgg tatcgagttt gaaattgatc gtgggtgccg
     3721 tgtcgcggtg cttggggata acggggaagg caagacgact ctgctcagga ccatgtcagg
     3781 ggatcttgcc cccaaacgcg gaagttttcg gtggggaacg ggtcttaaga tcgcgtttta
     3841 cgcgcagcat gttttttc
//
LOCUS       DAOSIO010000164         3824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_21014_length_3824_cov_3.771823, whole genome shotgun sequence.
ACCESSION   DAOSIO010000164 DAOSIO010000000
VERSION     DAOSIO010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21014_length_3824_cov_3.771823"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1483)
                     /locus_tag="PK590_06285"
     CDS             complement(<1..1483)
                     /locus_tag="PK590_06285"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOG24238.1"
                     /translation="MTGGSSAVYLFEDFNEQLNIPSHFLKRASCCPDKIALRYKIDNE
                     YSGSLTWRDWMERVRRVALGLYRIGIRKGDHVGILSENRFEWTLADLGALSLGAVVVP
                     VYATSSAEDIQYIFRHSGVRALFVSSREQLSKVAGLTDQMIANGLITFEPDDLTRTLS
                     LKDLEKWGHDEELNRDGIYEACLRDVSSDDLATIVYTSGTTGRPKGVMLTHRNFIANC
                     SGSRHYISLGENDVVVSFLPLAHIFERTAGYYFAAICGACIVYAESIRTVARDIAAVC
                     PTVVFAVPRFFEKAQSRVEEKLRQVAPVVRRIYNWALNIGKTATSQTETTGKVSFWIF
                     VAHGIAKSLLLRKIRCAFGGRIRFFVSGGAPLSSDLARFFHAAGLLILEGYGLTETSP
                     VIAVNTLGAFRFGSVGRPIPNIRVRIAQDGEIITSGPCLMRGYYRDPEGTAASMKQGG
                     FYTGDIGCLDSDGFLHVTDRKKDIIVTAGGKNVAPANIENRILS"
     gene            complement(1495..2211)
                     /locus_tag="PK590_06290"
     CDS             complement(1495..2211)
                     /locus_tag="PK590_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24239.1"
                     /translation="MIPVLALFVAVHLGAVGGEKSSGAAQNSATAKATAAASPQTFLS
                     EQFAKFFGKKSTKKTASAKQAKSFSTSPEKDKQSKDSVAAPKDEWLEKKVSDDASSVP
                     VVPATVSSAYVPPPPAVDLPPQVTQIRQEVQRVLQLNAQIKNLQGSQVVQVQRIQEQA
                     RMHQRILNKIETTPTVFSTGKGAEKEVLLAQEKLRIINNETQRNQAILRDLQKTSQMA
                     KKPESVTAYPTAVQLPVKQN"
     gene            complement(2333..3514)
                     /locus_tag="PK590_06295"
     CDS             complement(2333..3514)
                     /locus_tag="PK590_06295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOG24240.1"
                     /translation="MKDVVIAGACRTPIAKLGGTLKSFSSQDLGAQILRGLLERSKLD
                     PSEVDEVILGCVGQQSDAHNVARVIAVRAGIPHAVPAYTVNRNCGSGLQSIHNAFLSI
                     RSGESELIVAGGVEVMSASPFLNRDLRFGKKIRDSVLVDSLWEGLRDPLSGMMMGETA
                     ELLAKKSGIGRSEQDAFALESHQKACLADQKGMFKREIMPVVILDPEGRSNIFDRDEG
                     PRQETSLEGLGKLSPVFRKTDGTVTAGNSSQISDGAAAVIVASREKARSLGLDVMATI
                     REVAFAGIEPERMGLGPVIATKKALAKSGLSLKDIQTIEVNEAFAVQYLAVERELNLD
                     RSVTNCCGGAIALGHPVGASGARIVVTLLYEMERRQATLGLATLCIGGGQGGSMILER
                     C"
     gene            complement(3548..>3824)
                     /locus_tag="PK590_06300"
     CDS             complement(3548..>3824)
                     /locus_tag="PK590_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24241.1"
                     /translation="QNQLSWRRMMIKTLDRLTRRYTGNIFNVLMNTIQFMEYEIARMQ
                     ANDADIFIHAARSDGHWIEFFSPDKFIQEGIVRTREQLAEMKRLLVE"
BASE COUNT          970 a         1075 c          852 g          927 t
ORIGIN      
        1 cagaaagaat acgattttcg atgttcgccg gcgcgacatt ttttcctccg gcggtcacga
       61 taatatcttt cttccgatcc gtaacgtgga gaaaaccatc cgaatcaaga cagccaatgt
      121 ctcccgtata aaatcctccc tgtttcatcg aagcagccgt accctcagga tcccggtagt
      181 agccccgcat caggcagggg cctgaggtta taatctctcc gtcctgggca attcgaaccc
      241 ggatattcgg gatgggacgg ccaacgcttc caaatctgaa agctcccagc gtattaaccg
      301 caatcacagg ggacgtttcc gtgaggccat atccctcaag aatcaataac cctgccgcgt
      361 gaaaaaatct ggcaagatca ctcgaaagcg gagctcctcc agaaacaaaa aaacgaattc
      421 tgcctccgaa agcacatctt atttttcgca gcaaaaggct cttcgcaatc ccatgagcca
      481 caaagatcca gaaactcacc ttcccagtcg tttcagtttg tgaagtagcc gtcttcccta
      541 tgttaagggc ccagttgtat atgcgccgaa ccacgggagc tacctgtctg agcttttcct
      601 ccacccggga ctgggctttt tcaaaaaaac gagggacagc gaaaactacc gtggggcaaa
      661 cagccgcgat gtcccgggca accgtccgaa tactttctgc gtagactatg cacgcgccgc
      721 agatcgccgc gaaataataa ccggcggttc gctcaaaaat gtgggccaaa gggagaaaag
      781 acaccaccac gtcattttct ccgagcgaga tatagtgacg ggagccggag caattggcga
      841 taaaattccg gtgcgtcagc atgactccct tggggcgacc ggtagtgcct gaggtataga
      901 caatcgtcgc caaatcatca gaacttacat cccgaaggca cgcctcgtaa attccgtccc
      961 ggttcaattc ttcatcatgt ccccattttt caagatcctt gagagacaat gtccgggtca
     1021 aatcgtcagg ctcaaaagta atcaggccat tcgcaatcat ctgatccgtc aaaccagcga
     1081 cctttgaaag ctgttcccgc gatgaaacga aaagggctcg aacccccgaa tgccgaaaaa
     1141 tgtattgaat gtcttcggcc gaggaagtcg cataaacagg gaccactacg gccccaagac
     1201 tcagggcccc aagatccgct aacgtccact caaaacgatt ttcggaaaga atcccgacgt
     1261 ggtccccttt tcgaattccg attcggtaaa gacccaaagc aacgcgccgg acacgttcca
     1321 tccagtcacg ccaggtgagc gagcccgaat attcgttatc gattttatag cggagggcaa
     1381 ttttgtcggg acagcaactg gcccgcttca aaaaatggga cgggatattt aactgttcgt
     1441 taaaatcttc gaaaaggtag acggcggacg aaccacctgt catgggaatc aaatttagtt
     1501 ttgtttgacc ggtaattgaa ccgccgtcgg ataggcggtc acagactccg gttttttagc
     1561 catctgggac gttttctgta aatcacgaag aattgcctga ttgcgttgtg tctcattgtt
     1621 gataatcctg agtttttcct gggccagcaa aacttctttt tcagctccct tgcccgtgga
     1681 gaaaacagtg ggggtcgtct cgatcttgtt aagaattctt tgatgcatgc gagcctgctc
     1741 ctgaatacgc tgaacctgga caacctgact gccttgcaga tttttgatct gtgcattcaa
     1801 ctgcaatacc ctctgaacct cctggcggat ttgtgtcacc tgcggcggaa gatcaacagc
     1861 cgggggcggc ggaacatagg cgctggaaac cgtcgcagga accacaggaa cggaagaagc
     1921 atcatctgaa accttcttct ccagccattc atcctttggt gccgcaacag agtcttttga
     1981 ttgcttgtct ttctctggag aggttgagaa tgacttcgcc tgtttggcag aggccgtttt
     2041 ttttgtcgac ttctttccaa aaaatttggc gaattgctca ctaagaaatg tctggggaga
     2101 tgccgctgca gtggcctttg cagtcgcaga attctgagcg gctccggaag atttctcacc
     2161 acctaccgcg cccaaatgca ctgccacgaa taacgccaga accggtatca tagtaatatt
     2221 tttattcaaa ttccacctca tctcagcatg ttaggagtta tcgtgcgagg tattatatcg
     2281 tataaatcct gtttttcttg ataaattatt cctgctctca ccattacccc tgtcagcacc
     2341 gttctaggat catggagcca ccttgtcctc ctccaataca aagtgtggcc agtcccaaag
     2401 tcgcttgccg acgctccatt tcataaagta aagtcaccac aatcctcgcc ccgctagcgc
     2461 ctaccggatg tcccaacgct atcgccccgc cacaacaatt cgtcaccgat cgatcgaggt
     2521 ttaactctct ctcgactgcc agatattgta cagcaaaggc ctcgttcact tcaatcgtct
     2581 gaatgtcctt aagagaaaga ccgcttttcg caagcgcttt cttggtcgct atcaccgggc
     2641 ccaaccccat ccgttcaggc tcaatgcccg cgaaagcaac ttccctgata gttgccatca
     2701 catcaagccc cagagatcgg gctttttccc gagacgccac aatcaccgcc gccgctccat
     2761 ccgaaatctg gctggagtta cctgccgtca ccgttccatc cgtttttctg aaaaccggcg
     2821 acagtttacc aagaccttcc agactggttt cctgccgggg tccctcatcc ctatcaaaga
     2881 tattactccg tccttccgga tccagaatca caaccggcat tatttccctt ttaaacatcc
     2941 ccttttgatc agccaggcaa gctttctgat gagattccaa agcaaaggca tcttgttcgc
     3001 ttcggccaat ccctgacttt ttcgccaaaa gctccgcagt ctctcccatc atcattcccg
     3061 acagcggatc tcgcaaccct tcccacaggg agtcgacgag cacagaatcc ctaatctttt
     3121 tgccaaatcg aagatctcga ttaagaaagg gagaggccga cattacttca acaccacccg
     3181 ctacaatcag ctcactctcc cctgaacgaa tcgacaaaaa ggcattgtgt atcgattgca
     3241 atcctgatcc gcagttgcgg ttgaccgtat aagctggtac ggcatgaggg atgcccgccc
     3301 tgaccgcgat aacacgggct acattatgcg catcggattg ctgaccgaca cagcctaaaa
     3361 tcacttcatc aacttcggaa ggatcgagct ttgacctttc caaaagacct cgtaggatct
     3421 gtgccccgag atcctggctg gaaaaagatt taagcgtccc cccgagttta gcgatggggg
     3481 tgcgacatgc tcctgcgatc acaacatctt tcactggacg gtattcctcc gaatggattg
     3541 agagaggtta ttctaccaaa aggcgcttca tctcagccag ctgttccctc gttcggacga
     3601 tcccttcctg gataaactta tcaggtgaga aaaactcgat ccagtgtccg tcggaacgag
     3661 ccgcatggat aaaaatgtcg gcatcattcg cctgcattcg ggcaatttca tactccataa
     3721 attgaatggt attcatcaga acgttgaaaa tattcccggt gtaacggcga gtgaggcgat
     3781 ccagggtctt gatcatcatc cgccgccatg acaactgatt ctga
//
LOCUS       DAOSIO010000165         3816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_21062_length_3816_cov_4.910130, whole genome shotgun sequence.
ACCESSION   DAOSIO010000165 DAOSIO010000000
VERSION     DAOSIO010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21062_length_3816_cov_4.910130"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PK590_06305"
     CDS             <1..868
                     /locus_tag="PK590_06305"
                     /inference="COORDINATES: protein motif:HMM:NF023478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HOG24242.1"
                     /translation="QRKEFFGTITMNFGRVKVVSRVTLEERDLVFVVIQIGFYDFRCS
                     VMPFKDETSFEVLERDLFDALYQLPILDLSKKIDTYFGESYYALKDLRLGDRANIISR
                     LTHETIEKVSNFYENIYEENLRMNEIYRSINVPIPVEFRYAAEHTLSKRLMEAVRDWG
                     SQGFPLKKTLPIYRIIDSAKALDVQIHKEPISRHLSEELTSLVRKIVAQPSAELLEQA
                     VSILKLSKKIDVFLDKRVVQDELFAFVRSWKGILQAIPQVIREQEAHFLNLMEDLELS
                     TEKLRKIMSGNG"
     gene            990..3092
                     /locus_tag="PK590_06310"
     CDS             990..3092
                     /locus_tag="PK590_06310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG24243.1"
                     /translation="MSTANTEKKVLMSTLPGDDVRQVMWRFAERYEHQMVVQATRSVA
                     RGIVARLVADKQRTTHDWTPQKQEMLKAFDEAGVTAVFMEPEQGGYITGPKNLALALV
                     AFELAWVDAGAATASLANNLALEPIHEKGTPEQIHHYMSGCVPPKPGENREVLRGSFA
                     LTEPLPYVGVDAGMLSGRIRVAEWKEGEEPMIQVEKRGRFITNMGYANFVTAAVDTDD
                     PKIKSSCMVILEKGDEGVYDPGAPTLKLVHQLSCTHDPIFNLKIPASRIIGGYTIKDG
                     VIVPNYTHNEVIEAVFRRTRVTVAMMTSAKLLSAIEPVIRYQRTRYRGGDAAKPGTPR
                     YDLGIQQKEDAQHRLIDVWATGEASASLGFEAARLFDEFDPLEKQKDAKFLKEGIRGA
                     RAQMKVFAEPRKKALAYAVEVGKPEAQRDPKVIEAYESDFLVQYAVKESLADVFCPAC
                     KLWNTGYGATMMREAVSLMGGYGITEDCPGFLTQKWSDAQLEATYEGPECVQRRQMSL
                     TMTNEIFLAQFKNWIKDLRSLDASKPEIGAGTLAGAMELWLWSVEYLQNSKDANGKTL
                     YGGQRQGVTFPLSDALAWLLGSRQLILDTLELEKKGPENPTLAEGLPGFVQFYSDLSC
                     VQAARAAGEVAKICAELVYGYHAKGATCASPDAALKTFAELRGKLDCSLAGARLAKDR
                     AGEAAAQVMIPEALDYPL"
     gene            3173..>3816
                     /locus_tag="PK590_06315"
     CDS             3173..>3816
                     /locus_tag="PK590_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HOG24244.1"
                     /translation="MTDRQKMDVDIICAGFGPATAGFLTTLNRAMAEDPSAEHFQSKA
                     MPGMPLQVICYERADDIGFGVSGIVTEAKGIKASFTAEELAQIPMAHPVKHEEVYYLL
                     DPVGASRRSWLLKIVDAVLRIVGVILPWFKDHAFRLPFIPPFMAKHGGWVLSMGQFTQ
                     WLGSNIMGGGMIQIWPGMPVKEPLFDSNRVAGVRLVDQGVEKSGSPTPSDIISSR"
BASE COUNT          884 a          967 c         1085 g          880 t
ORIGIN      
        1 tcagcgcaaa gagttttttg gaacgatcac gatgaatttt ggccgcgtga aggtcgtgtc
       61 ccgagtcacg cttgaggaac gcgatcttgt gttcgtggtg atccagatcg gattctacga
      121 ttttcgatgt tccgtgatgc cgttcaagga tgagacgtcc ttcgaggttc ttgagaggga
      181 tctttttgac gcgctttacc agttgccgat ccttgatctc tccaaaaaaa tcgacaccta
      241 ttttggggaa tcttattacg cgcttaagga cctgcgactg ggggatcgcg cgaatatcat
      301 cagccggctg acccacgaaa cgatcgagaa ggtaagtaac ttttacgaaa atatttatga
      361 agaaaatctt cggatgaacg agatttatcg ctccattaat gtccccatcc ccgtggagtt
      421 tcgttacgcg gcggagcata cgctgagtaa acgtctcatg gaagcggttc gagactgggg
      481 ttcgcaggga ttcccgctca agaaaacact tccgatttac cgcattatcg attccgcgaa
      541 agcgcttgat gtccagattc acaaggagcc gatttcaagg cacttgagcg aggagctgac
      601 atcccttgtc cggaagatcg tggctcagcc gagcgctgaa ttacttgagc aagcagtttc
      661 tattctgaag ctttcgaaga aaatcgacgt ttttcttgat aagcgtgttg ttcaggacga
      721 actcttcgcg tttgttcgct cctggaaggg aattttgcaa gcgatcccgc aggttatccg
      781 cgagcaagag gcccattttc tgaatctgat ggaagatctc gagctttcaa cggaaaaact
      841 ccgtaagatc atgagcggga acggctgagg caaaatcgaa tttttccttg cctgggtcga
      901 gttccgcgct taatataccc gaacctcaaa aaaatgtgtc ctattatatg gactttctgg
      961 agagaacatt cgaaaaggag cccataccca tgtccacagc caatactgaa aaaaaagttt
     1021 taatgagtac gttgccggga gatgatgtcc gtcaggtgat gtggcggttc gcggagcgtt
     1081 acgaacacca gatggtggtc caggccaccc gttccgtggc ccggggaatt gtcgcccgtc
     1141 tcgtggcgga taaacagcgc accacgcacg actggactcc gcaaaagcag gaaatgctta
     1201 aggcttttga cgaggccggc gtgacggccg tctttatgga accggaacaa ggcggataca
     1261 ttacgggtcc caaaaatctc gcgcttgccc tggtggcttt tgagctcgcc tgggtcgatg
     1321 ctggcgccgc gaccgcgagc cttgccaata acctcgcgct tgagccgatt cacgaaaaag
     1381 ggacgcccga gcagattcat cactatatga gcggatgcgt accccccaaa ccgggcgaaa
     1441 atcgtgaggt gcttcgcggc tcgttcgcgc tcacggaacc cctgccttat gttggtgtcg
     1501 acgccggtat gctttcgggc cgtattcgcg tggcagagtg gaaagaaggc gaagagccca
     1561 tgatccaggt tgaaaaacgc ggccgattca tcacgaacat ggggtacgcg aattttgtca
     1621 cggccgcggt cgatacggat gatcccaaaa tcaaatcaag ctgtatggta attctcgaaa
     1681 agggggacga aggagtttac gatccggggg ccccgacgtt aaaacttgtt caccagcttt
     1741 cctgcacgca cgaccctatt tttaatttaa agattcccgc aagccggatc atcggcgggt
     1801 acacgatcaa agacggcgtg attgtcccga actacacgca taacgaagtg atcgaagctg
     1861 tgttccgccg gacgcgcgtg acggtggcga tgatgacgag cgcgaaactt ctttccgcga
     1921 ttgaacccgt gatccgctat cagcgtaccc gctatcgcgg cggtgacgcg gccaagcccg
     1981 gcactccgcg ttacgatctc ggcattcagc agaaagagga cgcccagcat cggttgatcg
     2041 acgtgtgggc aaccggcgaa gcgagcgcgt ccctgggatt tgaggccgcc cggcttttcg
     2101 atgaatttga tcctctcgaa aaacaaaaag acgcgaaatt tttaaaggaa gggatcagag
     2161 gcgcccgcgc ccagatgaaa gttttcgctg aaccgcgaaa aaaagcgctt gcctacgccg
     2221 tggaagtggg aaaacccgag gcacagcgcg atccgaaggt gatcgaagcc tatgaaagtg
     2281 attttctcgt gcagtatgcc gtgaaggaat ctctcgcgga tgttttttgt ccggcgtgta
     2341 agctctggaa tacgggctac ggcgcgacga tgatgcgcga agccgtcagt ttgatgggcg
     2401 gttacggcat cacggaagat tgcccgggat ttcttacgca gaagtggtcg gacgctcaac
     2461 tcgaagcgac ctacgagggt ccggaatgtg tccagcgccg ccagatgtcg cttaccatga
     2521 ccaatgaaat ttttctggcc cagtttaaaa actggatcaa ggacctgcgt tcactggacg
     2581 cctcgaaacc tgagataggc gccggaacgc tcgcgggagc gatggagctc tggctttggt
     2641 cggttgaata tctccaaaat tcaaaagacg cgaacggaaa gaccctttat ggcggtcagc
     2701 gtcagggcgt gacgttcccg ctgtcggacg cgcttgcgtg gctcctggga tcccgtcagc
     2761 tcattctcga tacgcttgag cttgagaaga aagggcccga gaatccgacg cttgcggaag
     2821 ggcttcccgg atttgttcag ttttacagcg atttaagctg tgtgcaggcg gcccgcgcgg
     2881 ctggtgaagt tgccaagatt tgtgccgagc ttgtttatgg ctatcacgcc aaaggcgcga
     2941 cttgcgcttc tccggacgcc gcgctcaaga ccttcgcgga attacgcggg aaactggatt
     3001 gctccctagc cggcgcgcgg ttggcgaagg atcgcgcggg cgaggcggcg gctcaggtca
     3061 tgattcccga agcgcttgat tatccgcttt aaactgtcac acaagccaca aggtcataaa
     3121 actacaagag aagttttcgt gtggcatgtg ccgtatagcc tgagaggccc ttatgactga
     3181 tcgtcaaaaa atggatgtcg atattatctg cgccggattt ggtcccgcca cagcgggttt
     3241 tcttaccaca ctcaaccggg cgatggcgga ggatccctcc gcggagcact ttcaaagtaa
     3301 ggccatgccc ggcatgccgc tccaggtcat ctgttacgag cgcgcggatg atattggttt
     3361 tggcgtttcg gggattgtga ccgaagcaaa aggcatcaag gcctctttta cggccgaaga
     3421 actcgcccag atcccgatgg cgcatcccgt caaacatgaa gaagtttatt atcttctgga
     3481 cccggtgggc gcgagccgga gatcctggct actcaaaatc gtggatgccg tccttcggat
     3541 tgtcggagtt atcttgccgt ggtttaaaga tcacgcgttc cgactcccgt ttatccctcc
     3601 gtttatggca aagcatggcg gctgggttct ttccatgggg cagttcaccc agtggcttgg
     3661 ttccaatatc atgggtggcg gtatgatcca gatatggccg ggtatgcctg tgaaagaacc
     3721 cttgtttgat tctaaccgtg tcgcgggcgt tcgccttgtt gaccagggag ttgaaaaatc
     3781 gggttcgcca acaccgagtg atataatatc gtcacg
//
LOCUS       DAOSIO010000166         3717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_21771_length_3717_cov_4.282359, whole genome shotgun sequence.
ACCESSION   DAOSIO010000166 DAOSIO010000000
VERSION     DAOSIO010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21771_length_3717_cov_4.282359"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..807
                     /locus_tag="PK590_06320"
     CDS             118..807
                     /locus_tag="PK590_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG24245.1"
                     /translation="MSSPEAVILSTVDLRKNYEMPTGSLEILRGVNLEVNRGEMLFVT
                     GKSGSGKSTLLHLLGGLDRPSGGRIFYQGRDLAKASERELAKIRSRNIGYVFQFYHLL
                     PELTVYENVLLPTLIVGKKNKDWVKEVMKRVKLWNRRTHFPSELSGGEKQRVAIARAL
                     ANRPEVVLCDEPTGNLDEETAGSIMTLLFELHRNEGHTFMIVTHDEELARMGTRVLKL
                     HEGQLVSPKPL"
     gene            844..1749
                     /gene="ilvE"
                     /locus_tag="PK590_06325"
     CDS             844..1749
                     /gene="ilvE"
                     /locus_tag="PK590_06325"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HOG24246.1"
                     /translation="MELQIYIDGKWYPKSEAKISVFDHGLLYGDGVFEGIRSYQGRVF
                     KLREHLDRLWESAHTIMLEIPMSQKEMEKAVIETLKRNKILNGYIRLVVTRGVGDLGL
                     SPWLCKRPTVFIITDNIALYPKELYQKGLAIITVPTVRNLPEAVNPSIKSLNYLNNIM
                     AKIEARNGGCMEALMLNHQGYVTECSGDNVFMIKRTGRGNSVRDYLLLTPPAYLGALK
                     GITRDAIIEVAQKIRLPFAERTLTRHDFYNADEVFLTGTAAEVIPVVKIDGRLIGEGN
                     PGKITKMLLRNFHELTRTDGVRYTL"
     gene            1848..2375
                     /locus_tag="PK590_06330"
     CDS             1848..2375
                     /locus_tag="PK590_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HOG24247.1"
                     /translation="MICNVCGTKEATIHLTEIVNGQMAEIHICERCAEEKGTDFKTYF
                     NLGDLLTGFAGMGEIAKSGGKKMPPIVCKSCGLTFEDFPKQGRLGCAHCYSVFKKPLE
                     VVIKQVQRSGYHVGKKPSKIPKEVRSAHDLRQLKERLKKSIRDEAFEESARLRDEIRQ
                     IEDKMKKISRKPKND"
     gene            2368..3432
                     /locus_tag="PK590_06335"
     CDS             2368..3432
                     /locus_tag="PK590_06335"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HOG24248.1"
                     /translation="MTDANHSPTLSVNWLKATGPESDVVVSSRVRLARNLFKYPFVFK
                     LSPEQRVAIISDVEKAVSGSSLLKKARLVQTKDLSDIEKQFFLERHLVSNELVQEDSP
                     GAVAITPDEMVSLMVMEEDHLRLQVFQSGLNLGEAWKIADDIDTELERELEYAFDATL
                     GYLTACPTNVGTGLRASCMLHLPSLVLTRQADKVLQALSKLNLAVRGLYGEGTQALGN
                     FFQFSNQITLGQPETEIISNLEGVIKQVIAHEREAREHLKKKRKSKMEDQVWRALGIL
                     KSARLISSQEAIQLLSLVQVGIDIGLLEGSVISRDLNQLFLWIQPGHLQKMNSGELSA
                     TERDERRAERIRAYFAKVTL"
     gene            3455..>3717
                     /locus_tag="PK590_06340"
     CDS             3455..>3717
                     /locus_tag="PK590_06340"
                     /inference="COORDINATES: protein motif:HMM:NF024987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4140 domain-containing protein"
                     /protein_id="HOG24249.1"
                     /translation="MKKSLIFSIALMFLAPAIGLADTVSTKSKISEVSVYAMDAFVTR
                     VASSEVKPGDTQILLEDIVAEFDPDSLRVKGHGTAAVKILGAQV"
BASE COUNT          934 a          824 c         1048 g          911 t
ORIGIN      
        1 aagttcacat gagtgatgtg gtgacgatag cgggggttgc cttcgcgctt gcggtaattg
       61 ccgcgcttta ccccgcgcac cgggccgcga gccttaagcc cgtggaggcg ctccgctatg
      121 agtagcccgg aagccgtaat tctcagcacg gtagatcttc ggaagaatta cgagatgccg
      181 accggttcgc ttgagattct tcgcggggtc aatctcgaag ttaatcgtgg agaaatgctt
      241 tttgtcacgg gaaagtccgg ttccgggaag tcgacccttc tgcatcttct tggagggctt
      301 gatcgtcctt ccggcggccg gatcttttat cagggtcgtg atctcgctaa ggcgtcggag
      361 cgcgaactgg caaagatccg tagtcgtaat atcggttacg tgtttcagtt ctaccatttg
      421 cttccggaat tgacggttta tgaaaacgtt ttacttccga cgctgattgt cggaaaaaag
      481 aacaaggact gggtcaagga ggtcatgaag cgggtcaagc tctggaaccg gagaacacat
      541 tttccatccg aactctccgg tggcgaaaaa cagcgagtcg cgatcgcgcg cgcccttgcg
      601 aatcgtccgg aggtcgtgct ttgcgacgaa ccgaccggaa atcttgatga agaaacggcg
      661 ggctctatca tgacattgct ttttgagctc catcgaaacg agggccacac ttttatgatt
      721 gtgacgcatg acgaggaact ggcccgaatg gggacgcgcg tgctcaagct tcacgaggga
      781 cagttggttt ctccgaagcc tctttaaaaa gagatcaatt tttattttac gagaggagac
      841 gttatggaac ttcagatcta tatcgacgga aaatggtatc ctaaaagcga ggccaagatt
      901 tcggtttttg atcatggtct tctctatggg gatggagtgt ttgaaggcat tcgctcctat
      961 caaggacgcg ttttcaaact tcgtgagcat ttggaccgtc tttgggaatc cgcccatacg
     1021 attatgcttg aaattccaat gtcccagaag gaaatggaaa aagcagtgat tgaaactttg
     1081 aaacggaaca aaattttgaa tggctatatt cgccttgtgg tgacccgggg ggtgggcgat
     1141 cttgggttgt caccctggct ttgcaaaagg cccacggtgt tcattattac ggacaatatt
     1201 gcgttgtatc cgaaggagct ctaccagaaa ggtctcgcga ttattacggt tccaaccgtc
     1261 cggaatcttc ccgaagccgt gaacccttcg attaaaagcc tgaattatct caacaacatc
     1321 atggccaaga ttgaagccag gaacggtggt tgcatggaag ccctgatgct caatcatcaa
     1381 gggtatgtta cggaatgttc gggagacaat gtttttatga tcaaacggac cggcagggga
     1441 aactcagtca gagattacct cctgctgacg cctcccgcct atctgggcgc gctcaagggg
     1501 attacgcgtg acgcgatcat tgaagtcgcg caaaagatca ggcttccgtt tgccgaacgt
     1561 acgctgaccc gacatgattt ttacaatgcc gatgaagtgt ttttgacggg gacggcggct
     1621 gaggtcattc cggtcgttaa aattgacggg cgcctgatcg gcgaagggaa tcccggaaaa
     1681 atcacaaaaa tgcttctccg gaactttcac gaactgaccc gaacggatgg agtgcgatat
     1741 accttgtgac gcgggagttt aaaatctgga tcctggattt ttaatgcccc gagttttttc
     1801 gatgtgaaaa aagtgaaaaa caatcctgaa aaagcgagat tgcctcaatg atttgtaatg
     1861 tatgcggtac gaaagaagcg accatccacc tcaccgaaat agttaatggc cagatggcgg
     1921 aaattcacat ttgtgagcgg tgcgcggaag agaaggggac cgattttaaa acctacttca
     1981 acctcggtga tttattgacg ggatttgcgg ggatggggga gatagccaaa agcggtggta
     2041 aaaaaatgcc gccgattgtc tgcaaatcct gcggactgac ttttgaggat ttccccaaac
     2101 aaggccggct tggttgtgcc cactgttata gtgtttttaa aaagccgctg gaggttgtca
     2161 ttaagcaggt gcaaaggtca ggctatcacg tggggaaaaa accctccaag ataccgaagg
     2221 aagtccgaag cgctcatgac cttcgacaac tgaaggagcg tctcaagaaa agcattcggg
     2281 acgaggcttt tgaggaatcg gctcgtctgc gggatgagat caggcagatt gaagataaga
     2341 tgaaaaagat ttcaaggaaa ccaaagaatg actgatgcga accattctcc gacgctgtcg
     2401 gtgaactggc ttaaggcgac gggccccgaa agtgacgttg tggtgagttc acgagttcgg
     2461 ttggcccgaa atcttttcaa ataccctttt gtgttcaaac tttctcccga acaaagggtt
     2521 gcgattattt cggacgtgga aaaagcggtg agcgggtcaa gcttactaaa aaaggcccgt
     2581 ttggttcaga cgaaggatct tagcgacatt gaaaagcaat tctttctgga gcggcatctt
     2641 gtttcgaatg agttggtaca agaggattct cccggtgccg tggcgattac tccggatgaa
     2701 atggttagtc tgatggtgat ggaagaagat cacttgcggc ttcaggtttt tcaatcggga
     2761 ctgaatcttg gcgaagcctg gaagattgcg gatgatattg atacggagct tgagcgggaa
     2821 cttgagtatg cttttgacgc gacactggga tacctgacag cctgcccgac gaatgtcgga
     2881 acggggcttc gcgcgtcctg catgcttcat cttccgagct tggtcttgac cagacaggcc
     2941 gacaaagtac tccaggccct ctccaagctg aatctcgccg tgcgcggcct ttacggtgag
     3001 gggacgcagg ccctgggcaa ttttttccag ttttcgaacc agatcacgct tgggcagccc
     3061 gaaacggaaa ttatttcgaa tctggaaggg gtgatcaagc aggtcatcgc tcatgagcgt
     3121 gaggcccggg agcacttgaa gaagaaacgg aagtcaaaaa tggaagatca ggtttggcgg
     3181 gctctcggga ttttgaaatc cgcgcgattg atttcatccc aggaagccat ccagttgctt
     3241 tctcttgtcc aggttgggat cgatatcggc cttttggaag ggtcggtcat ttcccgggat
     3301 ttgaatcagc tttttctctg gattcagccg ggacatctcc agaaaatgaa tagtggcgaa
     3361 ttgagcgcga ccgaacgtga cgagcggcgc gctgaacgta ttcgcgccta tttcgcgaaa
     3421 gtaacgctgt aaaaatgtaa aggggaaccg ggttatgaaa aaaagtctga ttttttcgat
     3481 tgcgttaatg tttttggcgc ctgcgatagg actagcggac acggtgagca cgaagtctaa
     3541 aatcagcgaa gtgtccgttt acgcgatgga cgcgttcgtg acgcgggtcg cgtcctcaga
     3601 agttaaaccg ggtgacaccc agattctcct ggaggacatt gtcgcggaat ttgacccgga
     3661 ttcgcttcga gttaaaggac atgggaccgc tgccgtcaaa attctcggag cgcaggt
//
LOCUS       DAOSIO010000167         3706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_21871_length_3706_cov_4.611613, whole genome shotgun sequence.
ACCESSION   DAOSIO010000167 DAOSIO010000000
VERSION     DAOSIO010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21871_length_3706_cov_4.611613"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="PK590_06345"
     CDS             <1..952
                     /locus_tag="PK590_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HOG24250.1"
                     /translation="LYLDYSRKPGEWEPNVYGGRENLEAVAFLKKFNEVVHARYPGIL
                     TIAEESTAWPNVTRPTFSGGLGFDLKWNMGWMHDTLEYFSKDPVYRKFHHGNLTFSMW
                     YQHSENFVLPISHDEVVHGKGSLIGKMPGDDMQKFANLRLFLGYMFAHPGKKLLFMGN
                     DFAQWSEWNLHQSIDWHFLQFSRHRRINQVVKDLNRLYRDYPAFHEGDLTTDRFEWID
                     FSDEMNSVISFLRWSRDRKQLLIFTFNMTPVIRENYRIGVPWHGFYKEIFNSEAFDYG
                     GCGLGNQGGIHSENIPMHKRPHSLNCQLPPLGVNIFCFCP"
     gene            1144..3147
                     /locus_tag="PK590_06350"
     CDS             1144..3147
                     /locus_tag="PK590_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HOG24251.1"
                     /translation="MKRKRTKKNIALSSRKSAAPEDVIPGIIIEDVRPSVDGGKYPVK
                     WIAGDPLTVKADIFKDGHDVIAAAVEYKKKRDKAWQSVPMKFLENDRWSGEFVPAENA
                     RYLYRVRAWMDPVASWMKHVEKKCQSEASVKSEVLEGIVLLEEARRLSKGKDRERIAT
                     LQGSLKRTSGVSHDVLALIRSSEFQKFLGGHSLRRLESLSVPLELVSDRRRARFGAWY
                     EIFPRSQGKKEGVSATFKDCIKRIPEIRQMGFDVLYLTPIHPIGVTKRKGPNNSLVAD
                     KGSPGSPWAIGSKEGGHKAIHPELGTMEDFKNLLKAAQRAGIEIALDIAFQCSPDHPY
                     VKEHPEWFFHRPDGTIHYAENPPKKYEDIYPLNFHCEDWRALWEELKSIILFWADKGV
                     KIFRIDNPHTKPLGFWKWIIEETQKVYPESIFFAEAFTRPKIMKFLAKAGFTQSYTYF
                     TWRNEKWELRQYLEELTQTEMKYYYRGNFFANTPDILHEYLQKGGRPAFMIRAILAAT
                     LSSSYGIYSGFELCENRAKAPGSEEYLDSEKYQYKVWDWDREGNIKALIAQVNHIRRE
                     NPALQGYDNLEFYDTRNEMILAYGKRTPDNANIIVTVVNLDPNHAQEDFVTLPLWKFG
                     IEDWQTYQMRDLLTGEKYYWKGPRNYVRLDPKAVPAHIFLLKK"
     gene            3166..>3706
                     /locus_tag="PK590_06355"
     CDS             3166..>3706
                     /locus_tag="PK590_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOG24252.1"
                     /translation="MEDHPLWYKDAIIYELHVRAFFDSDGDGIGDFQGLAAKLDYLQD
                     LGVTAIWLLPFYPSPQRDDGYDISDYENVNPAFGKLSDFKYFLREAKKRGLHIITELV
                     LNHTSDEHPWFKNARKALPGTKERNFYVWSETPEKYKDSRIIFKDYETANWEYDPVAK
                     AYFWHRFYSHQPDLNFDNPA"
BASE COUNT          968 a          815 c         1001 g          922 t
ORIGIN      
        1 tctgtatctc gattattccc ggaagcccgg agaatgggaa ccgaacgtgt acggaggcag
       61 ggagaacctc gaagcggtcg cgtttcttaa aaaattcaat gaggtcgtgc acgcccgcta
      121 tccggggatt ttgaccatcg ccgaggaatc taccgcttgg ccgaatgtga cccggccgac
      181 tttttcggga gggcttgggt ttgatttgaa atggaacatg gggtggatgc atgacacgct
      241 cgagtatttt tcaaaagatc cggtctacag gaaatttcat cacgggaacc tgactttttc
      301 catgtggtat cagcactccg agaatttcgt cttgcccatt tcgcacgatg aagtggttca
      361 cgggaaaggc tctttgattg gaaagatgcc gggggatgac atgcagaaat tcgcgaatct
      421 ccggttgttt ctcggttaca tgttcgcgca tccgggaaag aaactgcttt ttatgggaaa
      481 cgatttcgcc cagtggtcgg agtggaacct tcatcaatcc atcgactggc attttcttca
      541 gttttcccgg caccggagaa ttaaccaggt ggtgaaagac ctcaatcgtc tttatcgtga
      601 ttatcccgcg tttcacgagg gggatctcac caccgacaga tttgaatgga ttgatttttc
      661 ggatgaaatg aattcggtca tcagttttct tcgctggtca cgggacagga agcaattact
      721 catttttact tttaatatga ccccggtcat tagggaaaat taccggatcg gggttccatg
      781 gcacggtttc tataaggaga tttttaatag tgaagcgttt gattacggcg gatgcggttt
      841 aggtaaccaa ggggggattc attcggagaa tattccgatg cacaagcggc ctcactcctt
      901 gaattgtcaa ttgccgcctt tgggcgtcaa cattttctgt ttctgtccgt aaataaagca
      961 ggtcacgcga gtagacctgc gacgtgaaag cgaagcctgg accgggcaat ttcatgcggc
     1021 tgaaccgcag ttctcggacc gggtgtatgg atccatcgcg aagatacggg cggagcgaag
     1081 acaaagttat gctttggatc tgaaaatagg tatttaatat tttacagggg gcaaagagtc
     1141 gtaatgaaaa gaaaacgaac caaaaaaaat atcgcacttt catctcgcaa gtctgccgct
     1201 ccggaggacg tgattccggg catcatcatt gaggatgtcc gaccgtctgt cgatggaggg
     1261 aaatatcccg tgaaatggat cgccggggat ccgctgacgg tgaaggcgga tatttttaaa
     1321 gacgggcacg atgtgatagc ggccgctgtc gagtacaaaa agaaacgtga caaggcctgg
     1381 caaagtgttc cgatgaaatt cctcgagaat gaccgctgga gcggagagtt tgtccccgcg
     1441 gaaaatgccc ggtaccttta ccgcgttcgt gcgtggatgg atccagtcgc ttcctggatg
     1501 aagcacgtcg aaaaaaaatg ccaaagtgaa gcctcggtca aaagtgaagt tttggaggga
     1561 atcgttttac ttgaagaagc cagacggctt tcgaaaggta aggatcggga gcggattgca
     1621 actttgcagg ggagtttaaa acgaacctca ggggttagcc atgacgtgct tgccttgatc
     1681 cggtcgtcgg aatttcaaaa gtttttaggc ggacattcgc tcagaaggct tgaaagccta
     1741 tctgttccct tggagcttgt ttcggatcga cggcgggcca ggttcggggc atggtacgaa
     1801 atttttccga gatctcaggg aaaaaaagaa ggagtgagcg ctactttcaa ggattgtatc
     1861 aagaggatac ccgagatccg gcaaatgggc tttgatgtgc tttatttgac gccgattcat
     1921 ccgatcgggg tgacgaagag aaaaggaccg aataattccc ttgtggcgga taaaggttct
     1981 cccggatcgc cctgggcgat cggaagcaag gaggggggac ataaggccat tcatcccgaa
     2041 ctcggaacga tggaagattt taaaaatttg ctcaaggcgg cccaacgcgc gggtatcgaa
     2101 attgcgctgg atatcgcttt tcaatgttcg ccggatcatc cctacgtgaa agagcatccc
     2161 gaatggttct ttcatcggcc ggacggaacg attcactacg ctgaaaatcc ccccaaaaaa
     2221 tacgaagaca tttacccgtt gaattttcac tgcgaggact ggcgggcgct ttgggaggaa
     2281 ctaaaaagca ttatcctgtt ttgggcggat aaaggggtca agatcttccg gattgataac
     2341 ccgcacacga aaccgctggg tttctggaaa tggatcattg aggagaccca gaaggtgtat
     2401 ccggagtcga ttttttttgc ggaggctttc actcgtccca agatcatgaa gtttctggcc
     2461 aaggcgggtt ttacgcagtc ttatacttac ttcacctggc gcaatgaaaa atgggaactt
     2521 cggcagtacc tggaggagct aacgcagacg gagatgaaat attattatcg cggcaatttt
     2581 ttcgcgaaca ctcctgatat tttgcacgaa tacctccaga agggaggacg accggcgttt
     2641 atgattcggg cgattctcgc ggcgacactt tcttcctcgt acgggattta tagcgggttt
     2701 gaactttgcg agaatcgcgc gaaggccccg ggatctgaag aatatctgga ttcggaaaaa
     2761 taccagtaca aggtctggga ctgggaccgg gaggggaaca tcaaagcctt gatcgcgcag
     2821 gtgaaccata tccgccggga gaatcccgct ctccagggct acgataacct tgagttttat
     2881 gacacgcgca atgagatgat tcttgcgtac gggaaaagaa ctccggataa tgccaatatt
     2941 attgtgacgg tggttaacct ggatcccaat catgctcagg aagacttcgt gacgcttcct
     3001 ctttggaagt ttggcataga agactggcaa acgtatcaga tgcgggatct tttgacggga
     3061 gagaaatatt actggaaagg gccgcgcaat tatgtccgcc ttgatccgaa agcggtcccg
     3121 gctcatattt ttcttttgaa aaaataatcg gagaaaaagg aaaacatgga agatcatcca
     3181 ctttggtaca aagacgcgat tatttacgaa ctgcatgttc gcgctttttt tgacagcgat
     3241 ggtgatggta taggagattt ccaggggctc gcggccaagc tcgattactt gcaggatctt
     3301 ggcgtgacgg cgatctggct tcttccgttt tatccctcgc ctcaaagaga tgacggctat
     3361 gacatttctg attatgagaa cgtgaacccc gcgtttggaa aactctctga ctttaaatat
     3421 tttttgagag aagcgaaaaa acgcggtctt cacatcatta ccgaactggt attgaaccac
     3481 acgtcagatg agcatccctg gttcaaaaac gcgcgcaagg ctcttcccgg caccaaagag
     3541 cgaaattttt atgtctggag cgaaaccccg gaaaaataca aagactcccg aatcattttc
     3601 aaagattacg aaactgccaa ctgggagtac gacccggtcg cgaaggccta tttctggcac
     3661 cggttttact cacaccagcc ggatctaaat ttcgataatc ccgcgg
//
LOCUS       DAOSIO010000168         3688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_22019_length_3688_cov_3.649050, whole genome shotgun sequence.
ACCESSION   DAOSIO010000168 DAOSIO010000000
VERSION     DAOSIO010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22019_length_3688_cov_3.649050"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PK590_06360"
     CDS             complement(<1..310)
                     /locus_tag="PK590_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24253.1"
                     /translation="MPKAKKVSSKRAHEDVKKLWHINPLDMTKEGIKKSFASSLQYGR
                     AKDRYTATSRDNFMALGMALRDRLVERWIKTQQRYHRQKTKRVYYLYFEFLIGGLLGS
                     Y"
     gene            complement(471..1946)
                     /locus_tag="PK590_06365"
     CDS             complement(471..1946)
                     /locus_tag="PK590_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOG24254.1"
                     /translation="MKKHPHLLEINAFFFVSRMSEKYGRKLTLATIPDSEWRNFSKQG
                     FDYLWLMGVWKRSPWSRAHAIKEPGLRYAYELILGRWSEEDVGGSPYAIYDYELDPFL
                     GGIGELLQLKMKLNGMGMGLVLDFVPNHLAVDHPWVVNFPERFVTAKPSTVREHPEWF
                     FNNPIGRKIAHGRDPYFPPWCDTAQVNFWAEPLRHAWIETLLKIAKMADGVRCDMAML
                     GLNDVFDKVWGAHVKTPRPTDEFWGPAISRVKAQHPGFIFIGEVYWDMDWLLQQQGFD
                     FTYDKRLYDRMKHDAPESVSAHLDAHPDYRDKCLRFIENHDEDRAPVIFGRKKSQATA
                     VVTSTSPGLRFFHDGQCEGRRIRTPIHLVREPKEPADPEAVGFYQRLLEFIKGAVIHD
                     GEWSLLGVGKAWEKNESYRGLLAWLWHLEGHWKLVILNYSMIRAQGIIFLPDALKQQV
                     FIQFKEALTGQVYERTGSELARQGLYVDLEPWQSHLFDFKT"
     gene            complement(2147..2359)
                     /locus_tag="PK590_06370"
     CDS             complement(2147..2359)
                     /locus_tag="PK590_06370"
                     /inference="COORDINATES: protein motif:HMM:NF022229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOG24255.1"
                     /translation="MQAQYYAIGETAGKIYRFLEQKGEQTVTKVQSEIGADTAIFFQA
                     IGWLARENNINFNKLGKTLKISLINK"
     gene            complement(2404..>3688)
                     /locus_tag="PK590_06375"
     CDS             complement(2404..>3688)
                     /locus_tag="PK590_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOG24256.1"
                     /translation="FMPQFHETALHYFWEFTRAKDTFNNRYHRDITQAFRHFQDQGRL
                     EIITCGATHGFLPLISRTESAVRGQIETAVRLYEDVLGRRPIGIWLPECGYYPGVDKF
                     LKEQGIRYFLTDTHGILHASPRPKYGVFAPIFCPTGVAAFGRDVESSKQVWSAVEGYP
                     GDYDYREFYRDVGFDLDYDYIRPYISPDGIRVNTGIKYYRITGNTDDKEPYVRAWAIE
                     KAASHAANFMFNREKQIEHLYGFMGRKPLIVAPYDAELYGHWWYEGPDFLNFFIRKTA
                     FDQNVFRLIAPSDYFREYPVNQISTPSFSSWGWKGYSETWLSGSNDWIYRHIHKATLR
                     MSELAGRFRGQNGVVGRALKQAARELYLAQSSDWAFIMHTGTMVPYAIKRTKDHLVNF
                     TRLYDDLTNNRLDEGFLRELESKNNIFPNVDYHMF"
BASE COUNT          952 a         1013 c          820 g          903 t
ORIGIN      
        1 tataactgcc aagaagaccg ccgatcaaaa attcgaagta caaatagtaa acccgtttag
       61 tcttctggcg atggtaccgt tgctgagttt taatccatcg ctccaccaga cggtcacgca
      121 aggccatccc caaagccatg aaattgtccc gggaggtcgc cgtgtagcga tccttggcgc
      181 gtccgtattg gagactggag gcaaaggatt tcttgatccc ttccttggtc atgtccaggg
      241 gattgatgtg ccacaacttc ttgacgtctt catgggcccg ctttgaagaa actttttttg
      301 ctttcggcat tgtttcgctc acttttaata aaggttgaat tgtcaacaga tcacagggtc
      361 gccagcattt tcataattct caaaccgccg ctccctcttc atcccggaag ttcggaaaaa
      421 ctgatttgaa aacagattat cagatttgaa aaatatctcc aagtctcgat tcaggtttta
      481 aaatcaaaca aatgagattg ccatggttca agatcgacat aaagaccctg acgggcaagt
      541 tcactcccgg tgcgttcgta gacctgacct gtcagagctt ccttgaactg gataaaaacc
      601 tgctgtttca gcgcgtccgg taaaaaaata atcccctgcg cccgtatcat ggaataattg
      661 agaatcacaa gtttccagtg accttccagg tgccatagcc aggccaggag cccccggtag
      721 ctttcattct tttcccacgc cttgcctacc cccagcaaac tccattcccc gtcatggatc
      781 acagccccct tgataaactc aagcagacgt tggtaaaagc cgacggcctc cggatccgcg
      841 ggttccttcg gttcccgcac cagatggatc ggcgttctga tcctgcgccc ctcacattgt
      901 ccgtcatgaa aaaaacgcaa acccggcgat gtactggtga ccacagcagt cgcctgtgat
      961 ttttttctcc cgaaaatcac gggggccctg tcttcatcat gattttcaat aaaccggagg
     1021 cacttatccc ggtaatcagg gtgcgcgtcc agatgggcgc tcaccgattc gggggcgtca
     1081 tgtttcatgc gatcgtaaag gcgtttgtca taagtaaaat caaaaccctg ttgctggagc
     1141 aaccaatcca tatcccagta aacttctccg ataaatataa atccgggatg ttgggccttc
     1201 acccgggaaa tagccggccc ccaaaactca tctgtcggcc tcggggtctt gacatgagct
     1261 ccccagacct tgtcaaaaac atcgttcaac ccgagcatgg ccatgtcaca ccgcacacca
     1321 tccgccattt tagcaatttt aagcagggtc tcgatccacg cgtgacgcaa aggttcagcc
     1381 caaaagttca cctgcgccgt gtcacaccat ggcgggaaat aggggtcccg accgtgcgcg
     1441 attttcctcc cgatgggatt attaaaaaac cattcaggat gctcccggac ggtcgatggt
     1501 ttcgcggtta caaaacgttc cgggaaattg accacccagg gatgatccac ggcaagatga
     1561 ttggggacaa aatcgagcac aagccccatc cccattccgt ttaatttcat ttttagctgg
     1621 agaagttcgc cgatgccgcc aagaaaggga tccagctcat agtcataaat cgcgtacgga
     1681 gaaccaccga catcttcttc cgaccagcgg cccaggatca attcataggc gtaccgcaag
     1741 ccgggttctt tgatcgcatg agcgcgcgac cacgggcttc gcttccaaac tcccatcagc
     1801 cagagataat caaaaccctg cttcgagaaa tttcgccact ctgaatccgg gatcgtggcc
     1861 aaagtgagtt tgcgcccgta tttctcggac atccgggaga caaagaaaaa agcattaatt
     1921 tccagtaaat gggggtgttt tttcatcgta ttgatctcga cagtggatta tcggattttg
     1981 ttttaaatac cttgatacct ctacgccaaa ggtattttca tattcaaaaa gaacacaagg
     2041 cccctgatta aaaagccggg cttcatccga ttgataaagt ccggcctctt ttacacctta
     2101 tttatactcc gaaacgccac aggccacacc cgtaagtgta cccgaactac ttgttgatta
     2161 gcgaaatctt gagcgttttg cctaatttgt tgaaattaat attattttcc cgggccagcc
     2221 aaccaatcgc ctgaaaaaaa atagctgtat ccgccccaat ctcgctttgc accttcgtca
     2281 cggtctgttc gcccttctgc tcaagaaaac ggtaaatctt tcccgcggtt tcaccaattg
     2341 cgtaatattg tgcctgcatg atatttcctt tctcctgttc ttagggtcat gtatcccgaa
     2401 acattaaaac atgtgataat caacgttagg gaaaatgtta ttcttgctct ccaattcccg
     2461 aagaaaccct tcatctaacc gattgttcgt caggtcatcg taaagtcgcg taaaattcac
     2521 caagtgatcc ttcgttcgtt ttatggcgta aggtaccatc gttcccgtgt gcatgataaa
     2581 cgcccagtca ctggactgcg ccagataaag ctcccgggcc gcctgtttca gggcgcgtcc
     2641 caccacgcca ttttgtcccc ggaaacgtcc cgcgagctca ctcatgcgga gagtggcttt
     2701 gtgaatgtgc cggtaaatcc agtcattgga acccgaaagc catgtctcac tgtagccctt
     2761 ccagccccaa cttgaaaaag aaggcgtgga tatctggttc acgggatact ctcgaaaata
     2821 gtcggacggc gcgatcagac gaaaaacatt ttggtcaaac gccgtcttgc gaataaaaaa
     2881 gttaaggaaa tcaggccctt cataccacca gtgcccataa agctccgcgt catacggcgc
     2941 cacgatgagc ggtttccggc ccataaaacc gtaaagatgc tcgatctgtt tttcacgatt
     3001 aaacataaaa ttggcggcgt gagaggccgc cttctcaatg gcccaggccc tcacataagg
     3061 ttccttgtcg tccgtattcc ccgtgatccg gtaatatttg atccccgtgt tcacgcgaat
     3121 cccgtccggt gaaatgtagg gacggatgta atcataatcg agatcaaagc cgacatcccg
     3181 atagaactca cgataatcgt aatctcccgg atatccctcc acggccgacc agacctgttt
     3241 ggaagactcc acatcgcgtc caaacgccgc cactccggtc ggacagaaaa tcggcgcgaa
     3301 gacaccatat ttagggcggg gggacgcgtg taaaataccg tgcgtatcag tcaagaaata
     3361 acgaattccc tgttctttca agaatttatc gaccccggga taatacccgc actccggcaa
     3421 ccagatcccg atcggtcgcc gtcccaacac atcctcataa agtcttacgg ccgtctcaat
     3481 ctgtccccgc accgccgatt cggtgcgact gatcaggggt aaaaatccgt gcgtcgcgcc
     3541 gcaggtaata atttcgagac gcccttggtc ctgaaagtgg cgaaacgcct gtgtaatgtc
     3601 ccggtggtaa cgattattaa acgtatcttt ggcgcgcgta aattcccaga aataatgaag
     3661 agcggtctcg tgaaactgag gcataaac
//
LOCUS       DAOSIO010000169         3641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_22377_length_3641_cov_4.660625, whole genome shotgun sequence.
ACCESSION   DAOSIO010000169 DAOSIO010000000
VERSION     DAOSIO010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22377_length_3641_cov_4.660625"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PK590_06380"
     CDS             complement(<1..451)
                     /locus_tag="PK590_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOG24257.1"
                     /translation="MTQYDVVIVGGGPAGMMAALFARRTGKSVVILEKMQKLGKKLLI
                     TGAGRCNLSNRTLNSSFYKSEARPLVESVFRKFDGATILQYFKELGLHVYADETGRLF
                     PVTNQAASVLKVLELELARVKVSVECGFTVSEINPVGGDRRSGDSDFL"
     gene            complement(438..2363)
                     /locus_tag="PK590_06385"
     CDS             complement(438..2363)
                     /locus_tag="PK590_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOG24258.1"
                     /translation="MAHFKNQRGHDPKKQETILREGSHLKFLPTSSKDLEARGWEACD
                     IILVTGDAYVDHPAYGTALIGRVLESAGYKVGVIAQPDWRTDHDFLKLGRPRLFFGIS
                     SGNLDSMVANYTASKKPRRNDEYSPGGKGGLRPNRATIVYTSKIKGLFPGVPVVIGGI
                     EASLRRLAHYDYWEDHVRRSILLDSKADILVYGMAERQMSEIARRLNRADPICKLKET
                     ASLLHNIPGTVIVRSKNSLPAECRQDKSRVPSGDKVVEVPSYEEVVADKDQFNLAFKL
                     AYQESDPVRGKTILQKHGDRFIVQYPPAHPLSTKELDSVYELPFAYSWHPVYDRQGGV
                     PGFETTRFSITSHRGCSSECSFCSLYAHQGRIIQSRSKESILAEVRRMASRPDFRGTV
                     TDIGGPTANLYGAECGLWESAGACRLRQCMMPEKCQQLKLGYEKTIDLWRAVMKVPGV
                     KHLFIQSGLRYDLLIEKESDRYLQELCADHVSGQLKVAPEHVADRVLALMNKPSFEKY
                     KKFAERFENFSRKSGKKQYLVNYWISSHPGAGGHEAKKLAEYFNQKGIHPEQIQDFLP
                     LPATISGAMYWTGKHPMTGIPVFVARSYKEREAQRNLVQPSSRKMGYGKRQTKRTRHV
                     SFPVSRCPKKRNEHDTI"
     gene            complement(2402..3244)
                     /locus_tag="PK590_06390"
     CDS             complement(2402..3244)
                     /locus_tag="PK590_06390"
                     /inference="COORDINATES: protein motif:HMM:NF015892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG24259.1"
                     /translation="MKKFLIKMTIVFFLAGCLTVPSVSERRENADRIARENGFTRISI
                     RTVPFILTAYEKVNSPGSLVRIYIEGDGYAFVTRNRVSWDPTPRNPVAFRLAVQDDSA
                     NIVYLARPCQYTAHEKDVACDFPYWTSARFSDEVIRSMEMAVSSIVEKTQASGVELIG
                     YSGGAAVAVLIAERRSDVRSIRSVAGNLDSDAVNEYHGVTPLSESLNPVDSAEKIKQI
                     PQIHFSGLEDEIIPESALSSFVRRSGPEACLEIVRVRGADHETGWVESWPELLNIPFK
                     VCNK"
     gene            3400..>3641
                     /locus_tag="PK590_06395"
     CDS             3400..>3641
                     /locus_tag="PK590_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24260.1"
                     /translation="MNYHLLVHAGRDVLAIVPWLLGIYIFLELVEHKFGHVLANRISH
                     RNHIAPILGALFGCIPQCGFSVIASALYTRRCISLGT"
BASE COUNT          898 a          992 c          825 g          926 t
ORIGIN      
        1 aaagaaaatc cgaatctcca gatcgtctat ctcccccaac ggggttaatt tcgctaacag
       61 taaaaccaca ctccaccgaa accttcaccc tcgcgagctc aagctccaga accttaagga
      121 ctgacgcggc ctgatttgta accggaaaca atcttcccgt ttcatcggca taaacgtgaa
      181 gtcccagttc tttgaaatat tggaggatgg ttgccccatc aaatttccga aaaacagatt
      241 caactaacgg ccgggcttcg gatttataaa atgaggaatt aagggtcctg ttggaaagat
      301 tgcaacggcc cgcgcccgta attaaaagtt tttttccgag cttttgcatt ttttccaaaa
      361 tcacgacgga tttacccgtc cgcctcgcga aaagcgcggc catcatgccc gccggtcctc
      421 cgcctacgat taccacgtca tattgtgtca tgttcattcc gtttttttgg gcaacgcgaa
      481 acggggaacg aaacatgtcg ggttcttttc gtttggcgtt tcccgtaacc catttttctt
      541 gaggatggct gaactaaatt ccgttgcgct tcccgctcct tgtaggacct cgcgacaaaa
      601 accgggatcc ccgtcatcgg atgtttaccg gtccaataca tggcgcctga aatggtcgcc
      661 ggtagcggaa gaaaatcctg gatctgttca gggtgaatac ccttctgatt aaaatattcg
      721 gcaagctttt ttgcttcatg acctcccgcg cccggatgcg aactgatcca gtaattcaca
      781 agatactgtt tttttccgga tttgcggcta aaattttcga aacgctcggc aaacttttta
      841 tacttttcaa aactcggctt attcatcagc gcaagcaccc ggtcagcaac atgttccgga
      901 gccaccttga gctgaccgct cacatgatcc gcgcaaagtt cctgaagata ccggtccgat
      961 tctttttcaa tcagaagatc gtaccggagc ccgctctgga taaagagatg cttgaccccc
     1021 ggcactttca tgacggcccg ccaaagatcg atcgtctttt cgtatccaag cttaagctgt
     1081 tggcactttt ccggcatcat gcattgtcga aggcggcaag cgcccgcact ttcccaaaga
     1141 ccacattccg cgccatagag attggcggtc ggtcccccaa tatccgtcac ggtccctcta
     1201 aaatcagggc ggctcgccat cctgcggact tccgcgagga tggactcctt gctacgactt
     1261 tgaataatgc gcccctggtg cgcgtaaagc gaacagaagg agcactccga agaacacccc
     1321 cgatgggaag tgatcgaaaa ccgagtggtt tcaaaacccg gaacgcctcc ctgccggtca
     1381 taaacaggat gccacgagta tgcaaacgga agctcataaa cagaatcaag ctctttcgtc
     1441 gaaagggggt gtgccggcgg atactgaacg ataaatcggt cgccgtgttt ttgaaggatc
     1501 gtttttccac gaaccggatc cgactcctga taagccaatt tgaaagctaa attgaattga
     1561 tccttatccg cgacaacctc ctcatacgag ggcacttcca ccactttgtc tcccgagggt
     1621 acacgggatt tatcctgtcg gcattcagcc ggcaacgaat tcttgcttcg gacaatcacc
     1681 gtgccgggaa tattatggag gagagaggcc gtctctttta atttacaaat aggatccgcg
     1741 cggttcaatc tcctggcgat ctcggacatc tgcctttcag ccattccgta aacaaggata
     1801 tcagcctttg aatcaagcaa aatggagcga cgaacgtggt cttcccaata atcgtaatgc
     1861 gcaagacgac gaagacttgc ttcgatccct ccgatcacca cggggacccc ggggaaaagt
     1921 cctttgattt tgctcgtata aacgatcgtc gcgcgattgg gccggagacc gcccttccct
     1981 cccggcgagt attcgtcatt tctccgcggt tttttagaag ccgtgtagtt cgcgaccatc
     2041 gagtccagat tacccgaaga aatcccgaaa aaaagacgcg gtcggcccag cttgagaaaa
     2101 tcatggtccg tgcgccaatc tggctgggca atcacgccca ctttgtatcc cgcgctttca
     2161 agaactcgtc cgatcagggc ggtaccgtaa gccgggtggt cgacatacgc atcccccgtc
     2221 accagaataa tatcgcatgc ttcccagccc ctggcctcga gatcctttga acttgtcgga
     2281 agaaatttta aatgggaccc ttcccgaagg atagtttcct gttttttagg atcgtgaccc
     2341 ctttgattct taaaatgcgc cattcagttt tccttgctgt cttagatgaa aattgtaccg
     2401 tttatttgtt gcaaacttta aaagggatgt ttaaaagttc cggccacgat tcaacccatc
     2461 ccgtctcatg atccgcaccg cggacacgaa cgatctccag gcacgcctcg ggacccgacc
     2521 gtcggacaaa actacttaaa gctgattcag ggatgatttc atcttcaagc ccactaaaat
     2581 ggatctgcgg aatttgtttg attttttccg ctgaatcaac cggattaaga gactcagaca
     2641 acggcgtaac accgtgatac tcattgaccg catcagaatc cagattccct gcaaccgaac
     2701 ggatggagcg aacatctgat cttctttccg cgatcaaaac tgccaccgcc gccccgccgg
     2761 aatatccgat cagttccact ccagaggctt gagtcttttc cacaatggaa cttaccgcca
     2821 tctccatgga acgtatcact tcgtcagaaa atcgcgcgct tgtccaataa ggaaaatcac
     2881 acgccacgtc tttctcatgt gccgtatact gacagggccg ggcgagatag acaatgttgg
     2941 ctgaatcgtc ctgtacagca agcctgaagg caacgggatt ccttggcgtc ggatcccatg
     3001 agaccctgtt tctggtcaca aaggcatatc catccccttc aatgtagatt cggacaagac
     3061 tgccggggct gttgaccttt tcgtaagctg ttaaaataaa tggtactgtc cggatcgaga
     3121 tcctcgtaaa tccgttttct cgagcaatcc gatccgcatt ttcgcgccgc tcggaaactg
     3181 acggcactgt cagacaaccg gccaaaaaaa acacaatggt cattttaatt aaaaactttt
     3241 tcatggatgg aattatagtc gaaaatctga acgaaggtta tggttattgg ggtgtatccc
     3301 acttaaggca gtgaaaaagg gaacggtcaa ctgatccttc ccccctgttg aattttctga
     3361 gaaattgatt tttctctact ttcaagctag catgccctca tgaattatca tcttctggtt
     3421 cacgcagggc gggatgttct ggcgatcgtt ccctggcttt tgggaattta tattttcctc
     3481 gagcttgtgg aacataaatt cggccatgtg ctcgccaacc ggatcagcca ccgaaaccat
     3541 attgcgccga ttctcggcgc cttgttcggg tgcatccctc aatgcggctt ttcggtcatc
     3601 gcaagcgcgc tttatacccg tcgttgtatc tcgcttggga c
//
LOCUS       DAOSIO010000170         3629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_22470_length_3629_cov_3.217963, whole genome shotgun sequence.
ACCESSION   DAOSIO010000170 DAOSIO010000000
VERSION     DAOSIO010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22470_length_3629_cov_3.217963"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..841
                     /gene="rplD"
                     /locus_tag="PK590_06400"
     CDS             197..841
                     /gene="rplD"
                     /locus_tag="PK590_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOG24261.1"
                     /translation="MKVPLYTKEGKKKQEVVLNPEVFEVRINRRLLELVRNAYSANLR
                     RGTANTKTRGEVRGGGKKPWKQKGTGNARHGSTRSPIWKGGGVVFGPRPRDYSVDLSK
                     TIRGKALQIAVAMRGREKNIMLLESLKLATAKTKEWADIVKTLPLQGKKAVYLTKDLD
                     VNLKRASHNLQKWVCVKSVSDVNAYDILQHVKVIIDESALEPLEKRLMAGVSKS"
     gene            838..1125
                     /gene="rplW"
                     /locus_tag="PK590_06405"
     CDS             838..1125
                     /gene="rplW"
                     /locus_tag="PK590_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOG24262.1"
                     /translation="MSLTMYDIIVEPWITEKVTRATQSANQYGFKVHPNANKKEIKSA
                     VEKIFNVHVTRVNTVNVRGKWRRVRYQPGQTPDWKKAIVTLKKGDKIDWTK"
     gene            1164..1991
                     /gene="rplB"
                     /locus_tag="PK590_06410"
     CDS             1164..1991
                     /gene="rplB"
                     /locus_tag="PK590_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOG24263.1"
                     /translation="MGLIKFKPVTPTRRYGNASDFSDITVDRPFKPLTEIRKRTGGRN
                     NMGHITSRRIGGGHKQRIRLVDFRRLRHGIPATVLTVEYDPNRSARIALVAYQDGIKS
                     YILCPDGLKVGEQVVSGPEAEASLGNHLPLQKIPEGTMIHNIELKPGQGGKLVRSAGG
                     VAQILAKENDFAHIKLPSGEVRLIPAKAYATIGQCSNIDNENIVLGKAGRSRWLGKRP
                     ISRGVCRNPVDHPLGGGEGKSKGGNHPQSPWGQKSKGLKTRRRYKASNKYIVKDRRK"
     gene            1988..2287
                     /gene="rpsS"
                     /locus_tag="PK590_06415"
     CDS             1988..2287
                     /gene="rpsS"
                     /locus_tag="PK590_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOG24264.1"
                     /translation="MSRSSKKGVFVDEKLLKKVRTAVEKKDRRPIKTWSRRSTITPDF
                     VGVTLTVHNGKTFVNVFVTENMVGHKVGEFAPTRTFRKHSASGEKAAAMAAGAKT"
     gene            2306..2755
                     /gene="rplV"
                     /locus_tag="PK590_06420"
     CDS             2306..2755
                     /gene="rplV"
                     /locus_tag="PK590_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOG24265.1"
                     /translation="MAKADSKKTATQVAPDPRKVRSVTKHVRIAPRKLRLVINTIRKR
                     PAIEAVGMLSLMPQKGARIAIKTLKSAIANAKNLGLNERRLVVSDIRADGGPVMKRFI
                     SRSMGRANKILKRTSHLTVVVSEGRKIWGGESPEDVKSHKKKAAKVA"
     gene            2771..3526
                     /gene="rpsC"
                     /locus_tag="PK590_06425"
     CDS             2771..3526
                     /gene="rpsC"
                     /locus_tag="PK590_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOG24266.1"
                     /translation="MGQKAHPYGLRVGYIKPWRARWFAKGDAVAYLGEDLKIRKYLKS
                     KLATAGVSSIEIDRAASRLRVRLFAAKPGIIIGRRGQEIDKIKEDLTAITQNEVFLDI
                     KEVKMPQIDAQLVAENMALQLEKRVSFRRAMKKAVQLAMQKGALGIKVKCKGRLGGTE
                     IAREEGYKEGKVPLSTFRADIDYGFAEAFTTYGTIGCKVWIYKGDILIKKEQEEKKQE
                     LEKKLSQVEVEMKKAATEASEEVKENTDNAPDA"
     gene            3510..>3629
                     /locus_tag="PK590_06430"
     CDS             3510..>3629
                     /locus_tag="PK590_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOG24267.1"
                     /translation="MPLMPKRVKFRKQHRGWMGGVATRGSRLAYGDYGLKALEC"
BASE COUNT         1066 a          740 c         1034 g          789 t
ORIGIN      
        1 tgtcaagggg agtgtgccgg gttttgaggg tggatatgtt tttatcaaga catcactcaa
       61 acgcggcagt aaaaacaaat ggaaagtaag tcagcaaccg aaggaagctc ccgtagcttc
      121 gccggatgtt tcagctgaga aaaaagaaca cgatgacgcg aaataaggcg tccgatctga
      181 aaggtagagc agaacgatga aagtgcctct ttacacaaaa gaggggaaaa agaaacagga
      241 agtggtgctg aatcccgaag ttttcgaggt tcgcataaat cggcggcttc ttgaattagt
      301 gcgcaacgct tacagcgcca accttcgaag aggaacggca aacaccaaga ctcgcggaga
      361 agtacgcggg ggtggaaaaa aaccgtggaa acaaaaaggg accggtaatg cccggcatgg
      421 gtcgacccgt tcaccgattt ggaaaggcgg cggtgtcgtg tttgggccga ggcctcgtga
      481 ttattctgtg gatttgtcca aaactatccg gggcaaagcg ttgcagatag cggttgcgat
      541 gcgtggccgc gaaaaaaaca tcatgcttct cgagtcccta aagcttgcga cggcgaaaac
      601 taaggaatgg gccgatattg tcaaaacgtt gccgctacag ggaaagaaag ctgtttatct
      661 gaccaaagat ttggatgtga atttaaagcg cgcaagtcat aatttgcaga agtgggtttg
      721 tgtcaagagt gtttcggatg ttaatgctta cgacatactt cagcacgtga aagtcattat
      781 tgacgaatcg gcgcttgaac cgctcgaaaa aagattgatg gcaggagttt caaaatcatg
      841 agtctgacga tgtacgacat tattgtggaa ccgtggatca cggaaaaagt cacgcgggcg
      901 actcaaagtg cgaaccaata tggatttaag gtgcatccaa acgctaataa aaaagaaatc
      961 aagtctgcgg tggaaaaaat tttcaatgtt catgtcactc gcgtgaatac ggttaatgtg
     1021 agaggaaaat ggcgacgtgt gcgctaccag cccggacaga caccggattg gaaaaaagcg
     1081 attgtgacgt tgaaaaaagg cgacaaaatc gattggacga aataaccccc atgcgtgtgg
     1141 ggcaaggagc tagttaaata gttatgggac tcattaaatt caaaccagta acgccgaccc
     1201 gacgatacgg aaacgcgtct gatttctctg atattacggt ggatcgtcct tttaagccgc
     1261 ttaccgagat ccgtaaacgg accgggggac ggaataacat ggggcacatt acctcacgtc
     1321 gaattggggg tggtcacaaa cagagaatcc ggttagtgga ttttcgaaga ctgcggcacg
     1381 gtatacccgc gaccgtattg acggttgagt atgatcctaa ccggagcgcg cgtatcgcgc
     1441 tcgtggctta tcaggatgga atcaaaagtt acatcctttg cccggatggt ttgaaggtgg
     1501 gggagcaggt agtaagcggg cctgaggcgg aagcttcttt ggggaatcat ttgccgctac
     1561 agaaaattcc agaagggacc atgatccata atatcgaatt aaaaccgggt cagggcggca
     1621 agcttgttcg ctcagctgga ggcgtggctc aaattcttgc gaaggaaaat gattttgctc
     1681 atatcaaatt gccatcgggc gaggttcggt tgatccctgc caaagcctac gcgaccattg
     1741 ggcagtgttc gaatattgac aatgaaaata ttgttctcgg gaaggcggga cgcagtcgct
     1801 ggcttggcaa gcgtcctatt tcccgcggag tttgccgtaa ccctgtggat catccgctgg
     1861 gtggtggtga aggaaaatcg aaaggcggaa atcatccgca aagtccttgg gggcaaaaat
     1921 ccaaaggatt gaaaacacgt cggcggtaca aggcttccaa taaatacatc gtgaaggatc
     1981 gcagaaaatg agccgctcat ctaaaaaagg cgtttttgta gacgaaaaat tgctcaaaaa
     2041 agtcaggaca gccgttgaga aaaaagatcg ccggccgatc aagacatggt cgcggcgttc
     2101 cacgatcacc ccggattttg tgggggttac tttaacggtt cacaacggga agactttcgt
     2161 gaatgtgttc gtgaccgaaa acatggtagg tcataaagtg ggagaattcg ctccgacccg
     2221 aactttccga aagcacagcg cgtccggcga aaaagctgcc gcgatggcgg caggtgctaa
     2281 aacctaatcg atagaagaga gatttatggc aaaagctgac agtaaaaaaa cggctaccca
     2341 ggtggctccg gatcccagaa aagttagatc cgttaccaag catgtccgga tcgcgccgag
     2401 aaaattgcgt ctggttatca atacgatccg caagcgaccg gcgattgagg ccgtggggat
     2461 gctctcgctg atgccccaaa aaggcgcgag gattgccatt aagactttaa aaagcgccat
     2521 cgctaacgcg aaaaaccttg gattaaatga aagacggctc gttgtttctg acattcgagc
     2581 cgatggaggc ccagtcatga aacgcttcat aagtcgatca atggggcggg ccaacaaaat
     2641 tttaaaaaga acgtcacatc tgacggtggt ggtttctgag gggcggaaaa tttggggtgg
     2701 cgaatcaccg gaagatgtca agtctcataa gaaaaaggcc gcgaaagtag cttaaagagg
     2761 aggaaatact ttgggtcaaa aagctcatcc atatggttta agggtcggat atatcaaacc
     2821 ctggagagcg cgctggttcg ctaagggcga tgcggttgcg tatctgggcg aggatctcaa
     2881 gattcgtaag tatcttaaat cgaagcttgc gacggccgga gtttcttcga tcgaaattga
     2941 tcgcgccgcc agccgtttgc gggtcagact ctttgccgcg aaacccggga tcattattgg
     3001 gcgccgtggt caggagattg ataagatcaa ggaagatttg acagcgatca cgcaaaacga
     3061 agtctttctg gatatcaaag aagtcaagat gccccagatt gacgctcagc ttgtagcgga
     3121 gaatatggcg ttacagcttg aaaaacgggt cagttttcgt cgtgccatga aaaaagcggt
     3181 ccaattggcg atgcaaaaag gagcgcttgg aattaaagta aagtgtaaag gccgacttgg
     3241 tggcaccgaa attgctcgag aggaaggtta taaagagggc aaggtgcccc ttagtacatt
     3301 tcgcgcggat atcgattatg gttttgctga ggcctttacg acctatggta cgattggctg
     3361 caaggtttgg atctataagg gtgacatttt gatcaagaaa gaacaggaag agaagaagca
     3421 agaactggaa aagaaactca gtcaggttga agttgaaatg aagaaggctg ctactgaggc
     3481 ttccgaagaa gttaaggaga atacagacaa tgcccctgat gcctaaacgc gtaaaattcc
     3541 gcaagcaaca tcgcggttgg atggggggag ttgcgacccg gggaagtcgc ttggcttatg
     3601 gtgactacgg acttaaggcg ctcgaatgc
//
LOCUS       DAOSIO010000171         3583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_22816_length_3583_cov_3.482993, whole genome shotgun sequence.
ACCESSION   DAOSIO010000171 DAOSIO010000000
VERSION     DAOSIO010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22816_length_3583_cov_3.482993"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..297)
                     /locus_tag="PK590_06435"
     CDS             complement(100..297)
                     /locus_tag="PK590_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24268.1"
                     /translation="MKDKPKLQIIAQMSGYKNLKIEGGFRLQLDIFESRERDVAALAL
                     LVNRRRVVKITIEEYEEKDGN"
     gene            complement(287..466)
                     /locus_tag="PK590_06440"
     CDS             complement(287..466)
                     /locus_tag="PK590_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24269.1"
                     /translation="MRILIHTAEIRINGHERRVLASLRRSDVIAQAREWRREFQKPVR
                     IESFVSEVERPPHEG"
     gene            complement(585..1145)
                     /locus_tag="PK590_06445"
     CDS             complement(585..1145)
                     /locus_tag="PK590_06445"
                     /inference="COORDINATES: protein motif:HMM:NF040435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6475 domain-containing protein"
                     /protein_id="HOG24270.1"
                     /translation="MEQRDKKKFAALMAAMAEVFDRGAEISALKMDLYFKALERYPID
                     HISLAISNLIQGRVFPSLPKPAEIIQEIQGKAENRATEAWLLALEGIRRVGNYQSVKF
                     VDPVIHSVIQAMGGWPRFCDGEAKDEKWKQKEFERLYTVIAERPGKHPQYLPGDHELQ
                     NAALGHEQKQEIVQIGFEQEKIRLIK"
     gene            complement(1093..1848)
                     /locus_tag="PK590_06450"
     CDS             complement(1093..1848)
                     /locus_tag="PK590_06450"
                     /inference="COORDINATES: protein motif:HMM:NF016381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HOG24271.1"
                     /translation="MANPQVEDGHIDIANEIAEHLCRYRISGEEWMVLWAIIRKTYGW
                     RKKEDRIALSQFAAMTGLKRQTALRAINKLSSKKIIVVIKNDDSQINTYRFNKDFDTW
                     EPLSKKITLSSKKITTVTKKDNQVSSKKIHTKDNSTKDTLTKDNTPMPPWVPEAVFNE
                     YQASRKKKLRPASFKKFFAKLQRLSIESRASPEDILNQSIENGWEGIFKLKNDNGTGK
                     NKHAGIKEWLDQQGVSDGAEGQKEIRGTDGGDG"
     gene            complement(1926..2171)
                     /locus_tag="PK590_06455"
     CDS             complement(1926..2171)
                     /locus_tag="PK590_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24272.1"
                     /translation="MERRTAKIFRRVTFEAAFGPPIVKVCEILGMRYHGYDTPGGGWT
                     SVKGKDCTPAEWIGYREKGRQKKMWCNKKHILEIEDM"
     gene            complement(2171..2341)
                     /locus_tag="PK590_06460"
     CDS             complement(2171..2341)
                     /locus_tag="PK590_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOG24273.1"
                     /translation="MEGTVKWFNEQKGYGFIEKDKGGSVFVRDAEEMPCPYRGCRFFK
                     LTEERAEELGIR"
     gene            complement(2365..3246)
                     /locus_tag="PK590_06465"
     CDS             complement(2365..3246)
                     /locus_tag="PK590_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HOG24274.1"
                     /translation="MGEGLIEPTIDDESYFRSGSGLYLPANYYPAGVPRERPLCMDLF
                     SGCGGFSLGMVRAGFDVIAALEWDPPAIHTYLVNLGAYPVQMVFITPEDRERTEKYLE
                     KEFKRIMKARPDFSDQGEGFRCGRLMSGSNRHRAALDWEGCRYMFVGDIRKITGQEIL
                     DVMGLQSGDLDCVCGGPPCQGFSTSGRQNVMDPRNSLVFEFAKLIVEMQPKTMIFENV
                     PGILDMVTPEGIPVMDAVCKILEDGNYGNYDALRKSLLASAGCGAALKGSKGTRKGEK
                     ERWTPKEKESAIQGGLF"
     gene            complement(3231..3443)
                     /locus_tag="PK590_06470"
     CDS             complement(3231..3443)
                     /locus_tag="PK590_06470"
                     /inference="COORDINATES: protein motif:HMM:NF024251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HOG24275.1"
                     /translation="MILPFGKYFGREMTSLPDDYLVWLAGGGAYYRNRRSTEIKWKVP
                     TEVWVEARRILEARGYTLKGERWEKD"
BASE COUNT          726 a         1085 c          781 g          991 t
ORIGIN      
        1 agatacctga cgggtagtcc ttcacgcttc aaaaacttaa ccgcattcca tgtcgacaga
       61 ccgagaaaat tcatgatgtt ttttcggccc acgcaccatt caatttccat ctttctcctc
      121 gtattcctcg attgtgatct tcacgactct tctccgattg acgagcaatg ccagagccgc
      181 gacatctctt tccctggact cgaagatatc gagctggagc ctgaagcctc cctcgatctt
      241 gagatttttg tatccgctca tttgagcgat gatctggagt tttggtttat ccttcatgcg
      301 gcggcctctc cacctcactg acaaaactct caatcctcac cggtttttga aactccctcc
      361 tccactcccg ggcctgagca atcacatccg atctgcggag cgatgccagc acccggcgtt
      421 catggccgtt gattcggatc tctgcggtgt ggatgaggat tcgcatgggt caccattcct
      481 tccgatcaag catgtcgatc ccatgctttt tcaaaaattc ccgctcctgc tcataggttt
      541 cggcgatcaa aacatatccg ccctcgacct cgattatttc cagatcattt gatgaggcgg
      601 attttttcct gctcgaatcc gatctgtacg atttcttgtt tctgctcatg ccccaatgcc
      661 gcattctgta gctcgtgatc tcccggcagg tattgcgggt gttttccagg ccgctccgca
      721 atcacggtgt agagacgttc gaattctttc tgcttccact tttcgtcctt tgcctcgccg
      781 tcgcagaacc gcggccagcc tcccattgcc tggatcacgg aatgaatcac gggatcgacg
      841 aatttcacgc tctggtaatt cccgactcgg cggattccct ccagtgccag gagccaggct
      901 tctgttgctc tgttctcggc cttaccctga atttcctgta tgatctcggc aggctttggc
      961 aacgatggga aaacccggcc ctggatgaga ttggaaatag ccagggaaat atgatcgatc
     1021 gggtatcgtt cgagcgcctt aaaatacagg tccattttga gcgccgatat ctcggctccc
     1081 cggtcaaaaa cctcagccat cgccgccatc agtgccgcga atttcttttt gtccctctgc
     1141 tccatcacta actccttgct gatcgagcca ttctttgatt ccagcgtgct tgttcttccc
     1201 tgttccgtta tcgtttttta gtttaaaaat accctcccat ccgttttcaa ttgattgatt
     1261 taaaatatct tccggcgatg ccctcgattc aatcgataga cgttgcaact ttgcgaaaaa
     1321 ctttttaaac gatgcgggtc taagcttctt tttcctggat gcctgatatt cgttgaaaac
     1381 cgcctctgga acccatgggg gcataggggt attgtctttt gttaaagtgt cttttgtact
     1441 attgtctttt gtatgtatct tttttgatga cacctgatta tcttttttgg ttacagtcgt
     1501 tatctttttt gatgacagtg taatcttttt tgataacggc tcccatgtgt cgaagtcttt
     1561 gttaaatcta tatgtattga tctgactgtc atcatttttg ataacaacta taatcttttt
     1621 tgatgacagt ttattgattg cccttagggc tgtttgacgt ttcagaccgg tcatggcggc
     1681 gaattgagaa agcgcaatac gatcctcttt tttccgccat ccgtaggtct tacggataat
     1741 cgcccacaat accatccact cctctccgct gatccgatac cggcataggt gttcggcgat
     1801 ctcgtttgcg atatcaatgt ggccgtcctc aacttgagga ttcgccatct actcccccac
     1861 cttcccactc atccaccatg ccagtccgag cacggcgacg aaaaatatga tgattacgat
     1921 gagcttcaca tgtcctcgat ttcaagaatg tgttttttgt tgcaccacat tttcttttgc
     1981 cgcccttttt cccggtagcc gatccattcc gccggcgtgc aatccttacc cttgacggat
     2041 gtccatcccc cgccaggagt gtcgtatccg tgataccgca tcccgagaat ttcgcagacc
     2101 ttcacgatcg gagggccgaa ggcggcctcg aaggtcaccc tccggaatat ctttgcggtt
     2161 cttctctcca tcacctaatc cccaactcct cagcccgctc ctctgtcagt ttaaaaaacc
     2221 tgcatccgcg gtacggacaa ggcatctctt cggcatcacg aacgaacacg cttccgccct
     2281 tgtccttttc gatgaagccg taacccttct gctcgttaaa ccacttcact gtgccttcca
     2341 cttaatggtc tcctttcagt gttttcaaaa caatcctccc tgaatagctg actccttttc
     2401 ctttggcgtc caccgctctt tctcgccctt ccgcgtcccc ttcgatccct tcagggctgc
     2461 cccgcaaccg gcgcttgcaa gcaaagactt ccggagcgcg tcgtaattcc cgtagttgcc
     2521 atcttccagg atcttgcaga ccgcgtccat tacagggatt ccctccggcg tcaccatgtc
     2581 caggatgccc ggaacattct cgaaaatcat ggtcttgggc tgcatctcga caatgagctt
     2641 cgcaaactca aagaccaggg aattccgcgg gtccatgaca ttttgccttc cggacgtgct
     2701 gaacccctgg cagggaggcc cgccgcagac gcaatccagg tctccggact gtaggcccat
     2761 tacgtcgagg atctcctgcc cggtgatctt ccggatatcg ccgacgaaca tataccggca
     2821 cccctcccag tccagggctg cgcgatggcg gttcgatccg ctcatgagcc taccgcatcg
     2881 aaatccttcc ccctggtccg agaagtccgg cctcgccttc atgatccgct taaactcttt
     2941 ttcgagatat ttttccgtcc gttcccggtc ctccggcgtg atgaatacca tctggactgg
     3001 gtaggctccg agattgacta agtaggtgtg gatggcaggg ggatcccatt ccagggcggc
     3061 gataacatcg aaaccggctc gaaccattcc gagactgaac ccaccgcagc cggaaaacag
     3121 gtccatgcag aggggccgtt cccgcgggac gcctgccggg taataattcg ccggcaggta
     3181 cagtccggag ccggaacgaa aataggattc gtcgtcaatg gttggctcta tcaatccttc
     3241 tcccacctct cccccttcag ggtgtatccc ctcgcttcca gaatcctccg ggcctcgacc
     3301 caaacttctg tcgggacctt ccacttgatt tccgtggatc gccgattcct gtaataggcc
     3361 ccgcctccgg ccagccaaac cagatagtca tccggcaggg aggtcatctc ccggccgaaa
     3421 tatttcccga aggggaggat cactcccacc tcacaaccaa caacgtgtgc ggattatcca
     3481 ccgaatgatg cactcggctc ccatcccagg attcgatctg ccggtcatca gcccacaata
     3541 tcccctccat cgcatcagcc acggattcca tgcagccctg tag
//
LOCUS       DAOSIO010000172         3553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_23053_length_3553_cov_4.549743, whole genome shotgun sequence.
ACCESSION   DAOSIO010000172 DAOSIO010000000
VERSION     DAOSIO010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23053_length_3553_cov_4.549743"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /gene="topA"
                     /locus_tag="PK590_06475"
     CDS             complement(<1..1309)
                     /gene="topA"
                     /locus_tag="PK590_06475"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOG24276.1"
                     /translation="MAKAKKKASKGKQAVGDSGAAHLVIVESPAKAKTINKFLGKDYQ
                     VEASMGHVRDLPKSRMGVDLEKDFTPEYIISPKNIKTAKRLKASAAGKKSVYLATDPD
                     REGEAISWHLAHIFREQDSKIDIKRVEFHEITQEAVARAFERPRDIGMNLVNAQQARR
                     ILDRIVGYQLSPLLWKKVGRGLSAGRVQSVALRFIVDREREIRKFVPQEYWTLDAKFS
                     PQEGDHQEKIFLSRLDRIGSEKPVFPNQAEVEKIKQELSVLSFEVSKIDKTERRRRPQ
                     APYTTSKLQQESYTRIGFSSVNTMRIAQKLYEGVELGNEGSVGLITYMRTDSVNIAPT
                     ARQEAIQFIGEKFGKDYLPSTPPVYKSRKGAQEAHEAIRPTSVYRTPDSVKEYLADDE
                     LKLYELIWRKFVASQMVPAIDEVTAIVILGGDKFWFKTTGTRNI"
     gene            complement(1418..1789)
                     /locus_tag="PK590_06480"
     CDS             complement(1418..1789)
                     /locus_tag="PK590_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24277.1"
                     /translation="MKKKKKVRKKSSLNEVLSLYAQVLDLRNHLKETLAKKVRNEDRI
                     TDLEIRVNLLTRLLTTLCIERFGFRVATLRRLVKRIESQALRDSQIYELELLYKKSSL
                     TPKKKASLAKENPEDPWRKIS"
     gene            complement(1943..2332)
                     /locus_tag="PK590_06485"
     CDS             complement(1943..2332)
                     /locus_tag="PK590_06485"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOG24278.1"
                     /translation="MDGLVPASKGKRFAAGLIDLVIIPIVLGIIIGLVLLAAPDAVRN
                     VVLICVNVGWLLFRDAVYSPGRAMVNIRIVSLTGEKVTWLQALIRNILLIIPFVLVIG
                     YILEIVMLIAKGHRLADGWAKTQVLEA"
     gene            2586..2705
                     /locus_tag="PK590_06490"
     CDS             2586..2705
                     /locus_tag="PK590_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOG24279.1"
                     /translation="MEKGHLKPVVDSVYPLRQVKQAMQRVPRRENFGKIILKP"
     gene            2807..>3553
                     /locus_tag="PK590_06495"
     CDS             2807..>3553
                     /locus_tag="PK590_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG24280.1"
                     /translation="MKRSRLYLSGIRLMTLSAVAFALMALCVKIAAVSIPPVQIVFFR
                     SFFGMLLVLPIIFKKKVPLWGQEKMKLIVRGVSGFLALILHFYTISKLGLGLAVTLNY
                     ISPIFAVIGAVFFLNEKPSLLVWGLILLSLVGVLLLNPAPLPAWQPEIWLALLSSVFA
                     AIAYITIRTIKHRESPLTIIFYFTMIATIGSVFFLRHWVWPDFYGWLLVAGVVVFSFY
                     GQLWMTTALRRAPPYLVTPFQYLHPVISFGF"
BASE COUNT          871 a          925 c          768 g          989 t
ORIGIN      
        1 aaatatttcg ggttccggtc gttttaaacc agaacttgtc tcccccgaga ataacgatgg
       61 ccgtcacttc atcaatcgcc ggaaccatct gggaagcgac aaactttctc cagatcaact
      121 cataaagttt caactcatca tccgcaagat actctttgac agaatcaggc gtccggtaaa
      181 ccgacgtggg gcggatggcc tcgtgagcct cctgggctcc tttgcgggat ttataaaccg
      241 gaggggtaga gggaagataa tcttttccga atttttcgcc aataaactga atcgcttcct
      301 gccgagccgt cggcgcgata ttcactgaat cggttctcat gtaagtgatc aacccgacac
      361 ttccttcgtt tcctaactcc acgccttcat agagcttttg ggcaattcgc atggtgttga
      421 cggaagaaaa tccgatacgg gtatacgact cctgctgaag cttgctggta gtgtaaggcg
      481 cttgagggcg gcgacgtctt tcggtcttgt caattttgga aacctcgaac gacaaaaccg
      541 aaagctcctg tttgattttt tccacttcag cctgattggg gaaaaccggt ttttctgatc
      601 cgatgcgatc taacctggaa agaaatattt tttcctgatg atccccctcc tggggtgaaa
      661 atttcgcatc aagggtccaa tattcctggg gaacgaattt tcgaatttca cgttccctgt
      721 caacaataaa ccgaagcgcc acggactgga cgcgaccggc gctcaacccc cggccgacct
      781 ttttccaaag aagcgggctc aactggtaac ctacgatgcg atcaagaatt cgtcgcgctt
      841 gttgcgcgtt aacgagattc attccgatgt cccgagggcg ctcaaaggca cgcgctacgg
      901 cctcctgagt aatctcgtga aattccaccc gcttgatatc aatcttcgaa tcctgttccc
      961 ggaaaatatg cgccaaatgc cagctaatcg cttctccttc gcgatccgga tcggtggcca
     1021 aataaacaga ttttttgccg gccgccgaag ccttaagccg ctttgccgtc ttgatatttt
     1081 taggagagat gatgtattcg ggcgtaaaat ccttttcaag atcaaccccc atgcggctct
     1141 tgggaaggtc gcgaacatgg cccatggaag cttctacttg gtaatctttg ccgagaaatt
     1201 tattgatcgt cttggccttg gccggagatt cgacaatcac aaggtgggcg gcccctgaat
     1261 cacctaccgc ctgctttcct tttgaagcct ttttctttgc ttttgccaca acaactcctt
     1321 tgataagtaa actggaagtt gggaattcta atcaaatact acttatttgt aaatttgatt
     1381 ttatcggact ttccgcaagt tatcccctag aaacaactta ggatatttta cgccacggat
     1441 cttccggatt ttctttggcg agagaggctt ttttcttagg agtcagagag gacttcttgt
     1501 aaagcaactc gagttcataa atttgtgaat cccgcaaagc ctggctttca atgcgtttca
     1561 caaggcgtct cagtgtcgcg acgcgaaacc caaacctttc gatacaaagc gttgtaagta
     1621 aacgggttag aaggttaacg cgaatttcaa gatctgtgat tcgatcttcg ttccggacct
     1681 ttttcgccag ggtctccttc agatgattcc tcaaatccag gacctgggcg taaagggaca
     1741 acacttcgtt gagcgaagac ttttttctaa ctttcttttt ctttttcatc tatctcctct
     1801 ctaaagtcaa ttatagcaca caggatttcg gggaaggcca ggcggtcaaa accactttat
     1861 aaaatttcag aatccgcgaa tgaacaaaaa aaatcctccc ccatgagatt tttcacgggg
     1921 gaggatttta aaagattgga tcttaagctt cgagcacctg tgttttggcc cagccatccg
     1981 ccaaacgatg gcctttcgcg atcaacatca cgatttcaag gatgtaacca atgaccagaa
     2041 caaagggaat aatcaaaaga atattccgga tcagcgcctg aagccacgtc actttttctc
     2101 cggtcaaact aacaatcctg atattgacca tcgcgcggcc gggagaataa accgcgtcac
     2161 ggaataacaa ccacccgaca ttcacacaaa tcagcaccac attcctcacg gcatcaggag
     2221 cggccaggag gaccaatcca atgattatgc caaggacgat cgggataatg acaaggtcga
     2281 tcagaccggc cgcaaatctt ttgcccttac tcgctggaac caatccatcc ataataaact
     2341 cctttcgttg agaaagcttt ttatgttaac aagacgtggt tttcacgtaa actctatcgg
     2401 ggaaagacca gcctgtcaaa acttttttcg caaatgatcc ttacaggtaa cggctctctt
     2461 aaaagaaact tgaatgattc cgaattctac tcgtttcaga cttcgtttcg cttcatcacc
     2521 atttttttgt tatactttcc ccttagaaac cctgccctat tcaatcccgc gaggaggatt
     2581 cactgatgga aaaaggccac ctgaaaccgg ttgtggacag cgtctaccct cttcggcaag
     2641 ttaaacaggc catgcaacgc gtgccacggc gcgaaaactt cggaaaaatc attttaaaac
     2701 catgacaaca aaaaactttc gccgaaaggc cggggcctat ggccttatga taaaatcttt
     2761 ttttttacgt tgaacaaaca acctctttct ccctaggtct tcaacgatga agcgatcccg
     2821 cctttatctt tccggaattc gtttgatgac gctgagcgcg gtcgcgttcg cgctgatggc
     2881 gctatgcgtc aagatcgccg cggtatcaat tccgcccgtg cagatcgtat tctttcgtag
     2941 cttcttcggc atgcttctcg tattaccgat tatctttaaa aaaaaggtcc cgctttgggg
     3001 acaagaaaag atgaaactga tcgtgcgggg tgtcagtggt tttttagcgt tgatccttca
     3061 tttttacacc atttcaaaac tcggtttggg gctggcggta accctcaact atatctcccc
     3121 catttttgcc gtcatcggag ccgtcttttt cctcaacgaa aaacccagcc tgttagtctg
     3181 ggggcttatc ctgctttcgt tggtgggcgt gcttttactc aatcccgctc cccttcccgc
     3241 ctggcaaccc gaaatctggc ttgcccttct gtcatccgtt tttgccgcga tcgcttatat
     3301 cacgatccgg accatcaagc atcgggaatc cccgctaacc attatttttt acttcacaat
     3361 gatcgcgacg atcggttccg tctttttttt gcgccactgg gtatggcccg atttttatgg
     3421 atggttgctc gtcgcaggcg tcgtggtctt ttctttttac ggacagctgt ggatgacaac
     3481 cgcgcttcgc cgggcgccac cttacctggt aacgcccttt cagtaccttc accccgtcat
     3541 ctctttcgga ttc
//
LOCUS       DAOSIO010000173         3499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_23454_length_3499_cov_4.083914, whole genome shotgun sequence.
ACCESSION   DAOSIO010000173 DAOSIO010000000
VERSION     DAOSIO010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23454_length_3499_cov_4.083914"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PK590_06500"
     CDS             <1..212
                     /locus_tag="PK590_06500"
                     /inference="COORDINATES: protein motif:HMM:NF040055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24281.1"
                     /translation="LKLRGQGDLLGTRQAGVPFFRVANLATDEAMLVTAREKAVDVTN
                     GLDLATLKNPNHWIQKYLEQMPKND"
     gene            225..824
                     /gene="rsmD"
                     /locus_tag="PK590_06505"
     CDS             225..824
                     /gene="rsmD"
                     /locus_tag="PK590_06505"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOG24282.1"
                     /translation="MRIISGKFKSRRIDFPKTRLTRPMTDRTKETLFNIVGSFVFGKH
                     VLDLYAGSGSIGLEALSRGALSVTFVDKAPWAKSCIQRNLASLGLENQGVILSLDVLA
                     GIRKLNKLGRSFPLIFVDPPFHKGLVSKTLFKLDEMDLVQPFGQVVVGHSKHEPIPHE
                     ELKKLKLVRTKQIGQANLSFLFRLESANEKTKSYISGEL"
     gene            787..1272
                     /gene="coaD"
                     /locus_tag="PK590_06510"
     CDS             787..1272
                     /gene="coaD"
                     /locus_tag="PK590_06510"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOG24283.1"
                     /translation="MKKRKAIYPGSFDPVTFGHLDLIERALSLFDSVIVVVASNPAKK
                     TLFTTRERIQFLQNAVKKYPSVRVEYWEGLTVDFAKKVGASTIIRGIRATSDFDYEFQ
                     MALTNRHLAPNIDTVFLMPSEHHFFLSSRLAKEIAQMRGDVTKHVPKLVARRLLEKFK
                     G"
     gene            1277..1738
                     /locus_tag="PK590_06515"
     CDS             1277..1738
                     /locus_tag="PK590_06515"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOG24284.1"
                     /translation="MLKFLIADFKEGVCVPVEGEYDPKNIEVEFPDFRYGEPVRVKGF
                     VEKSTGTLRFKGTLTTRLLQTCGRCLKEKQTPLCEDFDWIYETEGKEVIDPLDSIREL
                     LILDRPLAYLCGENCKGLCPVCGTDFNENTCKCRQSGYHSFPVIKKLKKES"
     gene            1740..1940
                     /gene="rpmF"
                     /locus_tag="PK590_06520"
     CDS             1740..1940
                     /gene="rpmF"
                     /locus_tag="PK590_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOG24285.1"
                     /translation="MANPKRRHSNTRTRLRRANDALKLKSATQCPQCKAYILPHRVCR
                     ACGYYKGKLVKTIKVKSKEKAS"
     gene            1940..2980
                     /gene="plsX"
                     /locus_tag="PK590_06525"
     CDS             1940..2980
                     /gene="plsX"
                     /locus_tag="PK590_06525"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOG24286.1"
                     /translation="MVRVAVDGMGGDHGPSVIVEGVVMAANDFKHLEIVLVGQQDILK
                     AELNKHKVLGGKITIVEATEVVGMGETPVAALKKKKNSSISICVDLLQKKEVDAAMSA
                     GNTGAMVAASLLNLGMLPGVKRPGIAITIPTLHGMCVTLDVGANLNPSAYELFQYAIM
                     ADVFTKNVIKKKRPSIGLLNIGEEESKGTDVLKGAYKLLRESSLNFIGNIEGRDFFTG
                     KADCIICDGFVGNVCLKMIESVLETSILLVNRELHKNPISAIGAWFLKPALANLRRDT
                     NYEEAGGAPLLGVNGNVIISHGISSARAIRNAIRVAGELVTTQVNEQIVEAMTPYLNP
                     SVANSSRNSLAE"
     gene            3004..>3499
                     /locus_tag="PK590_06530"
     CDS             3004..>3499
                     /locus_tag="PK590_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HOG24287.1"
                     /translation="MTAPYRAAISGLGAYLPEKVLTNFDLEKIVETTDEWIRTRTGIR
                     ERRIAASHQSSSDLALPAAKEAIRNAGLTVQDIDLIIVATISPDMLFPSTACYLQAKL
                     GARCGAFDMAAACSGFPYALAVAESFVRSGVYKHVLVVGAEAITKFINWKDRATCVLF
                     GDGAG"
BASE COUNT          879 a          825 c          942 g          853 t
ORIGIN      
        1 acctgaaact gcggggccag ggggatttgc tcgggacccg ccaggccggt gtcccgtttt
       61 ttcgggtggc gaatcttgcg acggatgaag cgatgctggt gaccgcaagg gaaaaagcgg
      121 tggacgtgac caacggactg gatctcgcga ctttaaaaaa tccgaatcac tggatacaaa
      181 aatatctcga acaaatgccc aaaaacgatt aaaatatcgc ccgcatgcga attatttctg
      241 gtaaattcaa atcccgtcgt attgattttc cgaaaacccg actcactcgg cccatgacgg
      301 atcgaaccaa agaaaccctg ttcaacattg tggggtcttt tgtgtttggg aagcatgtcc
      361 tagatctgta cgcgggctcg gggagcattg ggcttgaagc gctcagtcgc ggagctcttt
      421 ccgtgacctt tgtcgacaaa gcgccttggg cgaaaagctg tatccagcgg aatctggcgt
      481 cgctgggact cgaaaatcag ggagttatcc tttccctgga cgtcctcgcg ggaattcgta
      541 aactcaacaa gctgggacgg tcatttcccc ttattttcgt ggatccgccg ttccacaaag
      601 ggttggtgag caaaactctt ttcaaacttg atgagatgga tcttgtccag ccatttggac
      661 aggtggtggt cggacattcg aaacatgaac cgatcccgca cgaagaatta aaaaaactaa
      721 aacttgttcg aaccaaacag atcgggcaag caaatctctc gttcctattt cgcctggaat
      781 ccgcaaatga aaaaacgaaa agctatatat ccggggagct ttgatcctgt cacgttcggt
      841 catcttgacc tgattgagag ggcgctttcc ctgtttgaca gtgtgatcgt ggttgtcgcg
      901 tcaaatcccg cgaaaaagac gctttttacg acacgggagc ggatccagtt cctccagaat
      961 gccgtcaaaa aatatccttc ggtgagagtg gaatactggg aagggctgac ggtggatttc
     1021 gcgaaaaaag tgggggcctc caccatcatt cgcggaattc gggccacttc ggattttgat
     1081 tacgagtttc aaatggcgct gacgaaccgt catttggcgc ccaacattga tacggttttt
     1141 ttaatgccat ccgaacatca tttctttctt tcctcccgtc tcgcgaaaga gatcgctcag
     1201 atgcgggggg atgtcacaaa acatgtgccg aaacttgtcg cccggcgctt gctcgaaaaa
     1261 ttcaagggtt aattcgatgt tgaaatttct gatcgctgat ttcaaggaag gcgtttgcgt
     1321 gcccgtggag ggtgagtatg acccgaaaaa cattgaggtg gaatttcctg attttcgata
     1381 tggggaaccg gtccgggtta aagggtttgt ggaaaagagc acaggtacac tgcgatttaa
     1441 gggaactttg accacgcgat tactgcagac ctgcggacga tgtcttaagg aaaaacaaac
     1501 acctctttgc gaggattttg actggattta tgagactgag ggaaaagaag ttattgaccc
     1561 gctggattct attcgcgagt tgctgattct ggatcgtccg cttgcttatc tttgtggaga
     1621 aaattgcaag ggattatgtc cggtttgtgg cacggatttt aatgaaaata cttgcaaatg
     1681 tcggcaatct ggttatcatt catttcctgt tatcaaaaaa ttgaaaaagg agtcataaga
     1741 tggcaaatcc gaagaggcga cactcgaaca cacggacgag acttcgcagg gcgaacgacg
     1801 ctctgaagct taagtcagcg acccagtgcc cccagtgcaa ggcgtatatt cttccgcacc
     1861 gggtatgccg cgcatgcggt tattacaaag ggaagctggt taaaacgatc aaggttaaat
     1921 ccaaggaaaa ggcttcctaa tggttagagt tgccgtggac ggaatgggag gagaccacgg
     1981 cccatccgtt atcgttgaag gggtcgtgat ggcggccaac gatttcaagc atcttgagat
     2041 cgttttggtc ggccagcaag acattttgaa agcggagtta aacaagcaca aggttcttgg
     2101 cggcaaaatc acgattgtgg aagccaccga agtcgttgga atgggcgaaa cgcccgtggc
     2161 cgctctcaaa aagaaaaaaa attcatcaat tagcatttgc gtcgacctcc ttcagaaaaa
     2221 agaagtggac gcggccatgt ccgccggcaa tacgggggcc atggtagcgg cttctttgct
     2281 gaacctcgga atgcttcccg gggttaaacg tcccgggatc gccattacta ttcccaccct
     2341 ccacggaatg tgtgtgacgc tggacgtcgg ggccaacctc aatccttcgg cctatgagct
     2401 tttccagtac gcgatcatgg cggatgtctt caccaaaaat gtcatcaaga aaaaacgccc
     2461 ttccatcggt ctccttaaca ttggagagga agaatccaaa gggaccgacg tgttgaaagg
     2521 ggcctacaag cttttgcggg aatcatctct taattttatc ggaaatattg aagggcggga
     2581 cttttttacc ggcaaggcgg actgcatcat ctgtgacggt tttgtcggaa acgtctgtct
     2641 caagatgatt gagagtgttc ttgaaacttc aatattgctc gtcaaccggg agctccacaa
     2701 aaatcccatt tcagcgatcg gagcgtggtt cctgaaaccg gcgcttgcga accttcgacg
     2761 ggacaccaac tatgaagagg cggggggcgc gccacttctg ggcgttaacg gcaacgtcat
     2821 tatcagccat ggcatttctt cagcccgtgc gattcgtaac gcgattcgag tcgcgggaga
     2881 gcttgtgaca acccaagtca atgagcaaat tgtcgaggcc atgacgccat accttaatcc
     2941 ctcagttgcg aattcatcgc ggaattcgct tgccgaatga ttttgtaggg atcccttgcg
     3001 actatgacag caccttaccg agcggctatt tcagggcttg gagcctatct tcccgaaaaa
     3061 gttctcacca attttgatct cgaaaaaatt gtggagacga ccgacgaatg gattcgcacg
     3121 cgaaccggca ttcgggagcg acgcatcgcg gcgtctcacc agtcttcaag cgatctcgcg
     3181 ttgccggcgg ccaaggaggc gattcgaaat gccgggctga cggttcaaga cattgatctt
     3241 atcattgtcg cgaccatctc gccggacatg cttttcccgt ccacggcctg ttatttacag
     3301 gccaaactcg gcgcgaggtg cggcgctttc gatatggcgg cggcttgttc cggttttcca
     3361 tacgcgcttg cggtcgctga aagttttgtg agatcgggtg tctataagca tgtgcttgtg
     3421 gtgggcgcgg aagcaattac aaaatttatc aactggaaag accgggcgac ttgcgtgctt
     3481 ttcggagacg gggccgggg
//
LOCUS       DAOSIO010000174         3488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_23547_length_3488_cov_4.118846, whole genome shotgun sequence.
ACCESSION   DAOSIO010000174 DAOSIO010000000
VERSION     DAOSIO010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23547_length_3488_cov_4.118846"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..429)
                     /gene="rplR"
                     /locus_tag="PK590_06535"
     CDS             complement(67..429)
                     /gene="rplR"
                     /locus_tag="PK590_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOG24288.1"
                     /translation="MSNLKELGRVARHKRIRRKITGTAERPRVVVHRSLTSLQAQAID
                     DVSHKTVAACSSLSKEFLKQVTQKGKISVAEKLGESFAADLKKKGIKQIAFDRGGYLY
                     HGRVKAFADSIRKGGIQF"
     gene            complement(449..985)
                     /gene="rplF"
                     /locus_tag="PK590_06540"
     CDS             complement(449..985)
                     /gene="rplF"
                     /locus_tag="PK590_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOG24289.1"
                     /translation="MSRIGKKPVTIPEKVKVTIQGQNIQVQGPKGVLSMVAHPRVKVA
                     MEGNEVKVTRATNEGSDRALHGLTRALIQNMIVGVTQGYSKELDIVGVGVKAAVKGNV
                     LSMSLGFTHPVEYSFGKDVEIKCPKPTNIVVMGLDKQRVGQVAAEIRSFYKPEPYKGK
                     GIRYTGEYVRRKQGKAVG"
     gene            complement(1080..1481)
                     /gene="rpsH"
                     /locus_tag="PK590_06545"
     CDS             complement(1080..1481)
                     /gene="rpsH"
                     /locus_tag="PK590_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOG24290.1"
                     /translation="MSRSDFIGDFLTCIRNAAQAKKDKLTVPASSLTVRLAEILKEEG
                     FIENVKPFAEGEKRFARIHMKYIGAERKSAIQGIKRISKPGIRYYVGSNKIPKVQGGL
                     GVAILSTPKGVMTDRQARENKAGGEILCHVW"
     gene            complement(1582..1767)
                     /locus_tag="PK590_06550"
     CDS             complement(1582..1767)
                     /locus_tag="PK590_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011077818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOG24291.1"
                     /translation="MAKTCLVVKQKRTPKFKVRRYNRCQRCGRSRGYIRKFGICRICF
                     RELAHLGMLPGVVKSSW"
     gene            complement(1779..2345)
                     /gene="rplE"
                     /locus_tag="PK590_06555"
     CDS             complement(1779..2345)
                     /gene="rplE"
                     /locus_tag="PK590_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOG24292.1"
                     /translation="MKQKIAVETPRLLDVYRKEVVPALLKEFGLKNPMQAPRLEKIVI
                     NMGVKEGQEDIKILDQLAVELSRITGQKPVVTRAKKSISSFKLREGSPIGLKVTIRRA
                     RMYEFLDRLINVAMPRIRDFRGFSDKSFDQAGNYSLGLTEQIIFSEVEYDKIKKTQGM
                     DITFVTTAKNRKEGKKLLELLGFPFKKA"
     gene            complement(2350..2658)
                     /gene="rplX"
                     /locus_tag="PK590_06560"
     CDS             complement(2350..2658)
                     /gene="rplX"
                     /locus_tag="PK590_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017247051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOG24293.1"
                     /translation="MKIKKGDEVIVTKGKDKGKKGRVMRVYPAESKLLVEKINYRTVY
                     MRKSQENPKGGITKMEGKLPVANVKLVCPRSGKPTRVGYSILADGTKHRLAKKSGEVL
                     "
     gene            complement(2750..3118)
                     /gene="rplN"
                     /locus_tag="PK590_06565"
     CDS             complement(2750..3118)
                     /gene="rplN"
                     /locus_tag="PK590_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOG24294.1"
                     /translation="MIQVRTVLEIADNSGAKKVAMIGVLGRRGKRTAEVGDIITASVK
                     EALPNGAIKKGEVVKAVLVRSKSPIQRKDGSIVRFSSNAVVLIDNQKNPRGTRIFGPV
                     ARELRDANFSKIISLAPEVV"
     gene            complement(3115..3375)
                     /gene="rpsQ"
                     /locus_tag="PK590_06570"
     CDS             complement(3115..3375)
                     /gene="rpsQ"
                     /locus_tag="PK590_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOG24295.1"
                     /translation="MSEQEKTEPSKRVREGVVVSAKMKKTVVVSVTRLLRHPKFNKTI
                     RRQIRYAVHDERSEAKEGDRVQIMETRPLSKTKNWRLVKVIK"
BASE COUNT          836 a          980 c          678 g          994 t
ORIGIN      
        1 tcatacaaat tcgccacaaa ccattgaacc atttttacat atcttgcgcg cgaaagaaca
       61 agcgaactaa aactggatac cgcctttgcg aattgaatcc gcaaaagctt ttacccttcc
      121 atgatagagg taaccgccac ggtcaaacgc gatctgcttg atcccctttt tcttgaggtc
      181 tgccgcgaat gattctccga gcttctctgc cacggagatc tttcctttct gcgtaacctg
      241 cttgagaaac tctttgctaa gcgaagaaca ggcagcgacc gttttgtgag aaacatcgtc
      301 aatggcctga gcctgaagac tcgtgagcga acggtgcacc acaacccggg ggcgctccgc
      361 ggtacccgtg attttccggc gaattctctt gtgtcttgct actcgtccca attccttaag
      421 gtttgacatg ttttttcaag tccctagatt agccgaccgc tttaccttgt tttcggcgaa
      481 catactcgcc ggtgtatcga atacctttgc ctttgtaagg ctctggctta taaaaactac
      541 ggatctccgc cgcgacctgc cccacgcgct gtttatcaag tcccatcacc acaatattgg
      601 tcggcttggg gcatttaatc tcaacatcct ttccgaaaga atattcgact ggatgtgtaa
      661 aaccgagaga catcgagagc acgtttcctt tcacagccgc tttcacgcca acacccacga
      721 tatccagttc tttggaataa ccctgtgtga cacccacgat catattttgg atcagggcac
      781 gcgtcaaacc atgcagggca cgatcactgc cttcattcgt cgcgcgagtt acttttactt
      841 cattcccttc catcgcaacc ttgactcgcg gatgcgccac catggaaaga acacccttgg
      901 ggccctggac ctggatgttc tgcccctgga ttgtaacttt aactttttca ggtattgtga
      961 ccggtttctt cccgatacgc gacatacatt tctccgttaa catgggcccg aaaacaccct
     1021 gcgatccctt cgggctaaaa actttattta caataactca gttcgatttt caacaaacct
     1081 taccagacgt ggcaaagaat ctctccaccc gccttatttt cacgggcctg acgatccgtc
     1141 ataacacctt taggcgtcga caaaatcgca acacccagac ccccttgaac tttggggatt
     1201 ttattgctcc cgacataata acgaatgccc ggtttggaaa tacgcttgat tccctggatc
     1261 gccgactttc tctccgcgcc aatatacttc atgtgaattc gcgcaaatcg cttctcgcct
     1321 tcggcaaaag gcttcacgtt ctcaataaaa ccctcttctt tgagaatttc ggcaagacgc
     1381 accgtcaggc tggacgccgg caccgtcaac ttatcttttt ttgcctgagc cgcattacga
     1441 atacatgtta aaaaatcacc gataaaatcg gaacgtgaca tttttttctc catttactcc
     1501 cgtgagggag cgttcactct cttacaactc gacgcgattt actggacgca ggcgtcactt
     1561 cgcttataaa acttaacaaa cctaccaact cgatttcaca acacccggca acattccaag
     1621 atgagcaagc tcacgaaaac agattcggca aataccgaat ttacgaatgt atccgcggga
     1681 ccgtccgcat cgctggcaac ggttgtaccg gcgcacctta aacttcggag ttcgcttttg
     1741 tttaacaact agacaggtct ttgccatctg ttattccttt atgctttctt gaacgggaag
     1801 ccaagaagct cgagaagttt cttgccctct ttacgattct tggccgtcgt tacaaaagta
     1861 atatccatcc cctgagtttt tttaatcttg tcgtattcaa cttcagagaa aataatctgt
     1921 tccgtcaaac cgagagaata attaccggcc tgatcaaagc tcttatccga aaaacctcga
     1981 aagtcacgaa ttcgcggcat tgcgacatta atcaaccgat ccagaaactc atacattcga
     2041 gcgcgtcgaa tggtaacctt aaggccgatg ggcgagcctt cgcgaagttt gaaggaggag
     2101 atcgattttt tcgctctcgt cacaaccggt ttttgtccgg taattcttga aagttccaca
     2161 gcgagctggt caagaatctt gatatcctcc tggccttctt tcactcccat attaatgaca
     2221 atcttttcaa gtctgggcgc ctgcatgggg tttttcaaac caaactcctt caaaagcgca
     2281 ggaacgactt ccttacgata aacatcaagc aaacgaggtg tttcgacagc gattttttgt
     2341 ttcatatttt tataaaacct cacccgactt ttttgcgaga cggtgtttgg ttccgtcagc
     2401 aaggatcgaa taccccaccc gagtcggctt tccgctccga ggacaaacaa gctttacatt
     2461 cgcaaccgga agctttcctt ccattttcgt gatcccaccc ttgggatttt cctgactttt
     2521 cctcatatac acggtccggt agttgatctt ttctaccaga agcttactct ccgccggata
     2581 gacccgcatc acgcgacctt ttttcccttt gtcttttccc tttgtgacaa tgacttcatc
     2641 gcctttttta atcttcatga cttaccttgg tagaaaccca tgcctttctt tgttatagaa
     2701 aacaaacttg aaaatttttc tcgcgtccaa tcccgcggag ggaaccggcc tagacaacct
     2761 ccggagccag cgaaataatt ttcgaaaaat tagcgtcccg aagctcacgc gccacaggac
     2821 caaatattcg ggttccccgg ggatttttct ggttatcaat caaaacaacc gcgttcgatg
     2881 aaaaacgcac aatagaccca tccttgcgct gtatcggact cttggatctc acaagaaccg
     2941 ccttaacaac ttcgcctttt ttaatcgctc cattgggaag cgcctctttc accgaggccg
     3001 ttataatatc gcccacttcc gccgtccgct tcccccggcg acccaacacg ccaatcatgg
     3061 caaccttttt cgctccgcta ttgtcggcta tttcaagaac cgttcgcacc tgaatcattt
     3121 tatcaccttc acaagccgcc aatttttcgt cttcgaaagc ggtcgggttt ccataatttg
     3181 aacccggtca ccttctttcg cttcgctacg ctcatcatgc accgcgtaac gaatttgacg
     3241 tctgatcgtc ttattaaact tggggtgtcg caaaagacgt gtcacggaaa caacgaccgt
     3301 tttcttcatt tttgcgctta cgacaacgcc ttcccgaacg cgcttgctcg gctctgtttt
     3361 ttcctgttcg ctcatttctt tttcccggtt ctctggatct cgttaatgat tgtcaggatc
     3421 gtcgcgatat cccgcttggc ctgacccaat gtgttttgct gttcaagctt tcctgttttt
     3481 tgctgaaa
//
LOCUS       DAOSIO010000175         3475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_23653_length_3475_cov_3.838012, whole genome shotgun sequence.
ACCESSION   DAOSIO010000175 DAOSIO010000000
VERSION     DAOSIO010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23653_length_3475_cov_3.838012"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /gene="lnt"
                     /locus_tag="PK590_06575"
     CDS             <1..616
                     /gene="lnt"
                     /locus_tag="PK590_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOG24296.1"
                     /translation="AYLMDKSGGIRQRYDKLHLVPFGEYIPLKPLFFWLTPIADALGI
                     SDFSVGATSEIFRWAREEWPFGVLICFEDVLPSLARALTAQGASFIAVITNDAWFLDT
                     SAPFQHLQGSIFRAVENGVPVVRAANTGVSGFISRRGEVLKTVMDENQKKTFVTGFAT
                     YDLPLLTRPTVYRTIGWMFPYFVAGLLALLTIFMIRKNIHGSLS"
     gene            complement(891..1463)
                     /gene="efp"
                     /locus_tag="PK590_06580"
     CDS             complement(891..1463)
                     /gene="efp"
                     /locus_tag="PK590_06580"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOG24297.1"
                     /translation="MSQVPAKELEKKLYIYIDGTPYWVLDVKFASPTARGASTMAKAR
                     VRNLLTGAVLDKTFSTTEKFDLADVEKIPVMFLYASGDEYCFMDNSTYEQFSISAAKL
                     GEQRFYLKENFEAQALKLNGSVVSLELPVYVELAVTETDPGLKGATAQGRSLKNAKLE
                     TGIEVQVPSYIEIGDKVRVNTETGEVSGRA"
     gene            complement(1551..2309)
                     /locus_tag="PK590_06585"
     CDS             complement(1551..2309)
                     /locus_tag="PK590_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG24298.1"
                     /translation="MTQPLHGKTALITGASRGIGKATALALAEKGAQIAVNYLHNEVA
                     AKNTAEQIRALGVKAEIFQCNVGDLDALPSLIDSVLKSFGSIDILIHNAALGAFKPVH
                     KLKINQYDLSMDINARAFLALVQKVLPGMEAKKEGTILALSSLGSHRYIPNYGAIGIS
                     KAALETLVRYLAVELMPKGIRVNGVSGGLVETDALRAFPLFEVFKNEVVKRTPAGRVA
                     SPEDLARVIAFLCTPESSWIVGQVVIADGGLSLI"
     gene            2603..>3475
                     /locus_tag="PK590_06590"
     CDS             2603..>3475
                     /locus_tag="PK590_06590"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009058286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOG24299.1"
                     /translation="MSVFIGRDRKVRRAYGFDEIALVPGDVTINPEEVDISMPLGKMN
                     LPIPFLASAMDGVVDPKFAIAMGKLGGLAVLNLEGIYVRYDNYEAILDEISKATPEKA
                     TQLVQKLYVKPINEKLVAKRIREIKKAKVPCAVSAIPQRAERLGKIAEESGADVFVVQ
                     STVTSVRHISKAYQAVDFKKFCKKMKIPVILGNCVTYKVALQLAETGAAGLLVGIGPG
                     SACTTRGVLGIGVPQVTAIADCAAAREYHHKKTGKYVSIIADGGMSTGGDICKSIASG
                     ADGVMVGSAFARTKE"
BASE COUNT          792 a          835 c          950 g          898 t
ORIGIN      
        1 cgcttatttg atggacaaaa gcggtgggat tcgccagcgg tatgacaaac tgcaccttgt
       61 tccatttggg gaatatatcc cgcttaagcc gctctttttc tggctgacgc cgatcgcgga
      121 cgcgcttggg atcagtgatt tcagcgtcgg tgccacgtct gagatttttc ggtgggcgcg
      181 tgaggaatgg ccttttggtg tgctcatctg ttttgaagac gttcttccgt ctcttgcccg
      241 cgcccttacg gcgcaaggcg ccagttttat tgccgtgatt accaatgatg cctggtttct
      301 ggatacgtca gcgccctttc agcatcttca agggtcgata tttcgggcgg tcgaaaacgg
      361 ggtcccggtg gttcgggccg cgaatacggg agtgtctggt tttatctcga ggcggggaga
      421 agtgctgaaa accgtgatgg atgaaaatca gaaaaaaacc tttgtgacgg gttttgccac
      481 ttacgatctt ccgcttttga cccgtccgac ggtatatcga acgatcggct ggatgttccc
      541 ttattttgtc gccggattgc tcgccttgtt aaccattttt atgataagaa aaaatatcca
      601 tggatcactt tcgtaaagga gactcatcaa atcgggcggg gcgcaaggtt ccttgcgtct
      661 tgtcttccgc gttaggcgtc ttttttttaa tttttctgtt ggggggatgc gtgcgtgtta
      721 ccggtaccgc cggttactgg aaaacaggcg aggacggcaa aacaaaggcc aaacgagcaa
      781 cttttgacac cgccgattat gtcccgcttg gtccctcgcc ggggtccatt gatttttgac
      841 cggactgcgg tcgcgtcttt ccttaaggaa tgaattaaaa aagagtttgc ttaagcccgc
      901 cctgaaactt ccccggtttc ggtattgacg cggaccttgt ctccgatctc aatgtaagag
      961 gggacctgga cctcaattcc cgtttcgagt ttggcgtttt tgagcgaccg gccctgggcc
     1021 gtggctcctt ttagtcccgg atcggtttcg gttaccgcga gttccacgta gacggggagc
     1081 tccagggaca ccactgaacc gtttagcttc agcgcctggg cttcaaagtt ttccttgagg
     1141 taaaaccttt gctcacccag tttggccgcg ctaattgaaa attgctcata ggtcgaattg
     1201 tccataaagc agtattcatc ccctgaagcg taaagaaaca tcaccggaat cttttcgacg
     1261 tccgcgagat cgaatttttc cgttgtcgaa aatgttttgt caaggacggc gccggtcagc
     1321 aagtttctta ccctcgcttt ggccatcgtg gatgctccgc gcgcggtcgg agacgcgaat
     1381 ttaacatcaa ggacccaata aggcgtacca tcgatgtaaa tgtaaagttt cttttcaagt
     1441 tctttcgcgg gcacttggga cataaacggt ctccggttga ttggtatgag tgggtattgt
     1501 atacgagacg ccgctttcgg gaaattctga aattttcttc cgaaattttc ctagatgagc
     1561 gaaagcccgc catcggcgat cacgacttgt ccgacgatcc acgatgactc gggggtgcag
     1621 agaaaagcga tgacgcgcgc gaggtcttca ggcgaggcga cacggccggc tggcgttctc
     1681 ttaacgactt cgtttttgaa aacttcaaaa agaggaaatg cccggagagc gtcagtttcc
     1741 acgaggcccc ccgagacccc gttaacacga attccttttg gcatgagttc aacggcgaga
     1801 taacgaacca gggtttccag cgccgccttt gaaatcccga tggcgccgta attggggata
     1861 taacgatgcg aacccaggct ggaaagcgcc aaaatagtgc cttccttttt tgcttccatg
     1921 cccggaagga ctttttggac gagagccaga aacgccctgg cgttgatatc cattgaaaga
     1981 tcgtattgat tgattttgag tttgtgaacg ggcttgaacg ctccgagcgc ggcattatgt
     2041 atcaggatat caattgatcc aaaactcttg agtacggaat caatcagcga ggggagggca
     2101 tccagatctc cgacgttgca ttgaaatatc tcggctttca cgcccaaggc gcgaatttgt
     2161 tccgccgtgt ttttcgcggc aacctcgtta tgcagataat tcacggcgat ttgagcgccc
     2221 ttttccgcaa gcgcgagagc cgtcgctttt ccgatcccgc gggaggcgcc cgtgatgagc
     2281 gctgtttttc catggagagg ctgtgtcata gaccgagaga atagcaaaga atttccaaaa
     2341 tctcaaaata aatatccagt tttggtgaat ttcgcggggt gaacaaaatt tttctcgaca
     2401 aattacaatt cgctttagta tgataccgca atgggacact tagcgatatc cattcaacgt
     2461 attttacaag cacaaggcgc catgaataac ggaggctctt gtgctttttt tgttaccaca
     2521 aatcaaaatc acaatcactt taaaagggac gggcgacgga tgagcgccgg tccctttttt
     2581 attgtttaag gaggaatgta tcatgtcagt ttttattgga cgtgatcgta aggtgcgccg
     2641 cgcgtatggc tttgacgaaa tcgcccttgt gcccggtgat gttacgatca acccggaaga
     2701 agttgatatc agtatgccgc tcgggaagat gaatctcccg ataccgttcc ttgcgtcggc
     2761 gatggacgga gtggtggatc cgaaatttgc gattgccatg ggcaagctcg gaggtctcgc
     2821 ggtactgaac ctcgaaggta tttacgtgcg ctacgataat tacgaggcca ttcttgatga
     2881 gatttcgaaa gcgacgcccg agaaagcgac tcaacttgtc caaaaacttt atgtgaagcc
     2941 aatcaacgaa aagttggttg ccaaacgtat ccgggagatc aaaaaggcga aagtgccatg
     3001 cgctgtttcc gctattccgc aacgcgcgga gcgtttgggg aaaatcgcgg aagaatcggg
     3061 cgcggatgtt tttgtggtgc aatcgaccgt cacctccgtg cgccacattt ccaaagctta
     3121 ccaggcggtt gattttaaaa agttctgcaa gaagatgaag atacctgtca ttcttggaaa
     3181 ttgtgtcacc tacaaagtgg cgctccagct agctgagacc ggtgccgccg gacttctcgt
     3241 tggaattggc cccgggtccg cctgcacgac gcgcggtgtg ctcggaattg gcgtgccgca
     3301 ggtgacggct atcgcggatt gcgcggcggc acgtgaatat catcacaaaa agacggggaa
     3361 atacgtgtcg attattgcgg acggcggaat gtcgacgggg ggggatattt gcaagtcaat
     3421 cgccagcggc gccgatggcg tcatggtggg ctccgcgttc gcgcgcacca aggag
//
LOCUS       DAOSIO010000176         3468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_23731_length_3468_cov_3.581893, whole genome shotgun sequence.
ACCESSION   DAOSIO010000176 DAOSIO010000000
VERSION     DAOSIO010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23731_length_3468_cov_3.581893"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /gene="pstA"
                     /locus_tag="PK590_06595"
     CDS             <1..1056
                     /gene="pstA"
                     /locus_tag="PK590_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOG24300.1"
                     /translation="ERGLFAGDQKRVLEESLGRLKGDYQVLADRAGYLRQTQDGARLG
                     YRLPTGQEMEISTGKIVDFYFPNRLGMVEKCQFFLKRLWRFLTEEPREANTEGGVFPA
                     IFGTFVMTVLMSLAVTPFGVLAAIYLREYAREGFLLRAVRVAINNLAGVPSIVFGVFG
                     MGFFVYFVGGTVDRLFFKDALPTPTFGTGGILWASLTLALLTIPVVIVAAEEAIASVP
                     RGMREGSFACGASKWQTIQRIVLPAAAPGILTGVILAMARGASEVAPLMIVGVVKLAP
                     NLPIDGIFPWIHLERKFMHLGFHIYDLGFQSPDSDAAKPMVFATALLLITLVVVMNLG
                     AILIREHLRKKYATGAF"
     gene            1084..1944
                     /gene="pstB"
                     /locus_tag="PK590_06600"
     CDS             1084..1944
                     /gene="pstB"
                     /locus_tag="PK590_06600"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018624161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOG24301.1"
                     /translation="MKLCDLTREGSSVDVNDEIRILTSGQISTQQNPIPFETCITIRN
                     YSFFYGAQQALFDVNLEVPEKQVTSIIGPSGCGKSTLIRNINRMNDLIDLTRYSGDIL
                     VKGQSIYDDELEVTEIRKRIGMVFQKSNPFPKSIYENVIYSLRVSGQNNRDFLDEVVE
                     NSLKAAALWDEVKDRLHKSALELSGGQMQRLCIARAIANQPEILLMDEPCSALDPLAT
                     AKVEELIFELKKQYTIVIVTHNMQQAARVSDFTAYFYMGRLIEYGETKKIFTNPSKKE
                     TEDYITGRLG"
     gene            2010..2294
                     /locus_tag="PK590_06605"
     CDS             2010..2294
                     /locus_tag="PK590_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24302.1"
                     /translation="MTKWSEKIKNNMDFFAVWEGDHDYHTGSGETFTSRNGILMADVY
                     SPRRAEVHSAIKNRSNPERTAPFFLKGLLSGSRLSSEDRLLSDIRTLAGS"
     gene            2378..3040
                     /gene="phoU"
                     /locus_tag="PK590_06610"
     CDS             2378..3040
                     /gene="phoU"
                     /locus_tag="PK590_06610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOG24303.1"
                     /translation="MERRFLEELKVLREKLFQMGLIVEAMIEKAVNSLLKGDNSLGDE
                     VFADENMINALEIEIDEMGHALLALAQPVAVDLRLLTMILKINTDLERMGDHAVNIAQ
                     RSRAVAHVFQERVHFSVVQMSDLVQKILRDAMDCFMNENTELARRVLASDDQVDACDE
                     RLCAAMVEKMQEDPSCVHQALNFVMIGHNLERIADLANNIAENTIYYKQGRDVRHHYE
                     TT"
     gene            complement(3289..>3468)
                     /locus_tag="PK590_06615"
     CDS             complement(3289..>3468)
                     /locus_tag="PK590_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24304.1"
                     /translation="CSLGILFPGELSYLRPRGYSDPLRAKAKDSLIKETLQCHPAKQG
                     VLQSKWVGETPRPPV"
BASE COUNT          830 a          760 c         1000 g          878 t
ORIGIN      
        1 gaacgagggt tgttcgcggg cgatcaaaaa cgggttttag aagagtctct cggccggtta
       61 aaaggagatt atcaagttct tgcggatcgg gccggatatc tccggcaaac ccaggatggc
      121 gcccggcttg gttatcggct tcccacgggc caggaaatgg aaatctcgac agggaaaatt
      181 gtggattttt acttcccgaa ccggttgggg atggttgaaa aatgccagtt ttttctcaaa
      241 cggttatgga ggttcctcac ggaagaaccc cgagaggcca atacggaggg cggagttttt
      301 ccggctattt tcgggacctt tgtgatgacg gttctcatga gtttggctgt gacgcctttt
      361 ggagtgctgg ccgcgatcta tctgcgggaa tatgcccgcg aaggatttct tctgcgagcg
      421 gtccgggtgg ccatcaataa tctggcgggc gttccctcga tcgtgtttgg agtttttgga
      481 atgggatttt ttgtctactt tgtcggtggg acggttgacc gtcttttttt caaggacgcg
      541 cttccgacgc cgacttttgg gacgggcggg attttgtggg cctccctgac cctggcgctt
      601 ttgacgatcc cggtcgtgat tgtcgccgcg gaagaggcga tcgcttcggt cccacgggga
      661 atgcgcgagg ggtcttttgc gtgcggggcc tccaaatggc aaacgatcca gcgcattgtt
      721 cttccggccg cggctcccgg tattttgacc ggagtcatcc tcgcgatggc gcggggagcg
      781 agcgaagtgg ctcccttgat gatcgtggga gtcgtgaagc ttgcgcccaa tcttccgata
      841 gacggtattt tcccgtggat tcaccttgaa cggaaattta tgcatcttgg gtttcatatt
      901 tatgatctgg gttttcagtc gcccgattct gacgccgcga agccgatggt gtttgcgacg
      961 gcgcttttgt taattacgct ggttgtggtg atgaacctcg gagccatttt gatccgggaa
     1021 catttaagaa aaaaatacgc aacgggagct ttctaaattc gggaagcgcc cgggaggaaa
     1081 ctcatgaaac tgtgtgattt gacgagggag ggtagttcag tggatgtgaa tgatgagatc
     1141 cggattttga cgtccgggca aatttcgacg caacagaacc caattccgtt tgaaacctgc
     1201 attacgatac ggaattacag ctttttttat ggggcccaac aggccctttt tgacgttaat
     1261 ctcgaggtgc ctgagaaaca ggtcacttcg atcattggcc cttcgggatg cggcaagtcg
     1321 accctgatca ggaacatcaa ccggatgaat gacctgattg atctgacgag gtattccggc
     1381 gatatcctgg tcaaggggca aagtatttac gatgacgaac tggaagtgac cgagatccgg
     1441 aagcggatcg ggatggtttt tcaaaagtcc aacccgtttc cgaaatcgat ttacgaaaac
     1501 gttatctata gtctgcgggt atccgggcaa aataaccggg acttcctgga tgaggtggtt
     1561 gagaacagtc tgaaggcggc ggcgctctgg gacgaggtca aggatcgttt gcataaaagc
     1621 gcgttggagc tttccggcgg gcagatgcag aggctttgca tcgctcgagc cattgccaat
     1681 cagcccgaga tccttctgat ggacgaacct tgctcggcgc tggaccccct ggcgacggcc
     1741 aaggtcgaag aactgatttt tgagctgaag aaacaataca cgattgtgat tgtgactcac
     1801 aacatgcaac aggccgcccg cgtgtccgat tttacggcct atttttacat ggggcgcctg
     1861 atcgaatacg gagaaacgaa gaaaattttt accaatccct cgaaaaagga aacggaagat
     1921 tacattacgg ggagattggg atagctttgt atcgaatgtg gaatgttttg acatcatgtt
     1981 ttaatcgaaa caggggagcg tctgatgcta tgacgaagtg gagcgaaaaa ataaaaaaca
     2041 acatggattt tttcgcggtc tgggaagggg atcacgatta ccacaccggg tccggcgaaa
     2101 ccttcacgtc cagaaacggg atcctcatgg ctgacgttta tagcccaaga agggcagaag
     2161 tccattccgc aataaaaaat cgctcgaatc ccgagagaac cgcccccttt tttctgaagg
     2221 gacttttgag cggcagtcgc ttgagttcgg aggatcggtt actctcggac attcgcactc
     2281 tggcgggcag ctaggagaca gttctccgcg acgattgatt ttttagggga gtaggggaga
     2341 aataactccc acaacttgca gggggaaggg gattggcatg gaacgccgtt ttcttgagga
     2401 actgaaagtg ttacgcgaaa agcttttcca gatggggctg attgtcgaag cgatgattga
     2461 aaaagcagtg aactctctgc taaagggcga caacagtctt ggggatgaag tctttgccga
     2521 tgaaaacatg attaatgccc ttgagattga aattgatgaa atggggcatg cgttgcttgc
     2581 tttggcacag cccgttgcgg tggacctcag gttgctgacg atgattctca aaattaatac
     2641 ggacctcgaa agaatgggtg atcacgcggt gaatattgcg cagagaagcc gtgctgtggc
     2701 ccacgtgttt caggagcggg ttcatttttc ggtggtgcaa atgagcgatc ttgtccagaa
     2761 aatacttcgg gacgctatgg actgctttat gaatgagaat acagagttgg cgcggagagt
     2821 gctggcgagc gacgatcagg tggatgcctg tgatgagcgg ctttgcgcgg cgatggttga
     2881 gaaaatgcaa gaggatcctt cttgcgtgca tcaggcgctg aatttcgtga tgatcggaca
     2941 taatcttgaa cggatcgctg accttgccaa taacattgcg gaaaatacta tttattacaa
     3001 gcagggcagg gacgtaaggc atcattatga aacgacttaa aaaaatattt tggttttatc
     3061 ccgcctttta aaaagagcag aagacatttc ctcccgaaat tgatcagcgg aatcaaaaat
     3121 ccttcctgtt taaacattcg agctttttcc ggcggttaat ttgttgtttg gggccagttg
     3181 ccagatggca ttgactccag agcttgcccg gaaagctgaa atctcaaaaa atctcgcctc
     3241 cgtcggattt tcgtagattc attccgcagt cttgggtaaa ctatggcgct aaacgggggg
     3301 tcggggggtt tccccgaccc acttactctg aaggacgccc tgcttcgcgg ggtggcattg
     3361 aagagtttcc ttaatgagag aatccttcgc cttcgctctc aacgggtctg aataccctct
     3421 gggccgcaga taggataatt cccccggaaa taatatcccg agggaaca
//
LOCUS       DAOSIO010000177         3437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_23998_length_3437_cov_4.732407, whole genome shotgun sequence.
ACCESSION   DAOSIO010000177 DAOSIO010000000
VERSION     DAOSIO010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23998_length_3437_cov_4.732407"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PK590_06620"
     CDS             <1..338
                     /locus_tag="PK590_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24305.1"
                     /translation="RTEENLALVRLFKAFFNNKMTILIFLCRSDFYTPTAAGLNSVCH
                     FLDDLYRHMLDGHVQRQYRRKFIWIKGFLRKLPSVRPSQRTTALRMISSLYDCFGIDF
                     PLEREGVES"
     gene            342..1241
                     /locus_tag="PK590_06625"
     CDS             342..1241
                     /locus_tag="PK590_06625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG24306.1"
                     /translation="MTGSRSGCFAGGSGTGFFIGRFHPVKKGFARSDRNPGGIFMAVE
                     VGMEKLESRVEQSMPDMRHNLVNVLYTVKGYVETHLCQFKEGLFRSDDEALQHAREMM
                     KRIYTQTDKALLITKRIGLAMKALPKTPGDIKPTSIKDVWGEITAMLQRQRSESGCVE
                     IISHIADEFPNVLCDRSDLMEILYYLTDNAMQAMVDERNPALPGRKEKGKLIIRASLG
                     FRAKDAPTATITIADTGPGIPEEMLTRIFEPFVTTREQSGGNGLGLCMAHALVRRNGG
                     SIAVSSFKGCGTTFVLTFPVAYE"
     gene            complement(1436..2584)
                     /locus_tag="PK590_06630"
     CDS             complement(1436..2584)
                     /locus_tag="PK590_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOG24307.1"
                     /translation="MSDEPLYILGDTASGPVQKPSLPIKNQGAILGVLKAAVNHRASD
                     VHFQVGSHPIFRIDGRLSPFKGFPPVTDIVMTDCVNMFLKEGQRKHLAEKRQIDCSVS
                     LPDFTHRFRINFFFQRNHLSGAFRLNPIDPPTFEQIGLPSFLKKLVELPNGLVLITGA
                     TGTGKTTTLSALISYMNHHRNAHIITLSDPIEYVHKNKNCLISQREIRNDATSFAEAL
                     RVALREDPDVIVVEEIRDLETVAMALTAAETGHLVIATLHTNDSVQTIDRIIDIFPPH
                     QQQQVRVQLASSLQAVVSQILVRRKDGQGRVAIFETMPVTTPIRNLIRDGQTVQIYNI
                     MQTNRSQGMILLNDAINEQVQNQVISPEDAASYYINTTFFQNQQKNQR"
     gene            complement(2678..3046)
                     /locus_tag="PK590_06635"
     CDS             complement(2678..3046)
                     /locus_tag="PK590_06635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase system-associated protein, TIGR04073
                     family"
                     /protein_id="HOG24308.1"
                     /translation="MEGGIVRKNALVLFLGIALVAGFTASGFANETKEDAFTKFGRGF
                     SNILTSPFELYIQPMLMSKNHEPAQSIGGGLAKGFAMMLVRIAGGIYEIVTFPVPIPK
                     DYAPILNPATPFTDWNSRRP"
     gene            3178..>3437
                     /locus_tag="PK590_06640"
     CDS             3178..>3437
                     /locus_tag="PK590_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24309.1"
                     /translation="MRCFLMIVAILAIIVLAGGWWVWGNLPEIAGWWIEQNSRGALDG
                     PVEIGDIQWVGSGVRLEKIAGKLRTRQGPAPFVARSVASVDP"
BASE COUNT          806 a          748 c          974 g          909 t
ORIGIN      
        1 acagaaccga agagaatctc gctctcgtca ggctcttcaa ggcgtttttt aacaacaaga
       61 tgacgatatt gatttttttg tgtcgttctg atttttacac gcctaccgcg gctggcctga
      121 acagtgtttg tcatttcctg gacgatctct accgacatat gcttgatggt catgtgcaaa
      181 ggcaataccg gcggaaattt atatggatca aaggttttct ccgcaaattg ccgtccgttc
      241 ggccgtctca gcgaaccact gctcttcgaa tgatttcctc gctttacgat tgcttcggga
      301 ttgattttcc actcgagcgg gagggagttg agtcatgaaa catgacgggg agtcgctcgg
      361 gatgttttgc cggcggaagt ggaacaggat tttttattgg tcgttttcat cctgtcaaaa
      421 aagggtttgc gcgatcggat cggaacccgg ggggaatctt catggctgtt gaggtcggaa
      481 tggaaaaatt ggaatcaaga gtggagcagt cgatgccgga catgcggcat aatctggtaa
      541 atgttttata caccgtcaaa ggctatgtcg aaacccatct ttgccagttc aaggaaggac
      601 tgtttcgtag tgacgatgag gccctgcaac atgcccggga aatgatgaaa cgcatttata
      661 cccagaccga taaggcgctt ttgatcacga aaagaatcgg cctggccatg aaagcgctcc
      721 ccaaaacgcc cggtgatatc aaaccaactt caatcaagga tgtttggggg gagatcaccg
      781 caatgttgca aaggcagaga tcagaatccg gatgcgttga aattatcagt catattgctg
      841 atgaattccc taatgtgctt tgtgacagga gcgaccttat ggagattttg tattatctca
      901 cggacaatgc gatgcaagcc atggtggatg agagaaatcc ggcgcttccg ggacgaaaag
      961 aaaaagggaa attgattatc cgggcaagtc ttggtttccg ggccaaagat gcgccgacag
     1021 cgacgattac aattgccgat acgggacccg ggattccgga agagatgttg acccgtattt
     1081 ttgaaccttt tgtaacgacg cgggaacaat cgggcgggaa tggtcttggt ctttgcatgg
     1141 ctcatgcgct tgtccggagg aatggcggga gtatcgctgt ttcaagtttt aaaggatgcg
     1201 gcacgacttt tgtgctgaca tttccggtgg cttatgaatg acctccaaaa cgtttttttc
     1261 atgatggttc aaaattcaca cgaaaacctg ggggggggtt gggtcaactg attatgacgg
     1321 cagtcctcag cggaggggtt ccgtgggttg ggtggtcgtg atccggcctg gagcacgacc
     1381 accacgctga gttttttccc gtgctttccg gaaatatttc taaaagtatc gcgatttaac
     1441 gctggttttt ctgctggttt tggaaaaaag tcgtattgat gtaatacgat gcggcgtctt
     1501 ccggcgaaat gacctggttt tgcacctgtt cattgatagc gtcattgaga aggatcatgc
     1561 cctgtgaacg gttggtttgc atgatgttgt aaatttgcac ggtctggcca tcacgaatta
     1621 aattgcgtat tggggtcgtg accggcatgg tctcaaagat tgccactcgg ccctggccgt
     1681 cttttcgtct gacgaggatt tgtgagacga cggcttgcag ggaagaggca agctgaaccc
     1741 ggacctgttg ttgctggtgt ggcggaaaaa tatcaatgat ccggtcgatc gtctggaccg
     1801 aatcgttggt gtgcaatgtg gcgatgacca gatggcctgt ttcagcggcg gtcagggcca
     1861 tggcgacggt ctccagatcc cggatttcct cgacaacgat cacgtcagga tcttctctca
     1921 gcgcgacgcg cagggcttcc gcgaacgaag tggcgtcatt tcgtatttcc cgctgactga
     1981 tcaggcagtt tttgttttta tggacatact caatcgggtc agaaagcgtg ataatgtggg
     2041 cgtttcggtg atggttcatg taactgatga gagcggacag ggtggttgtt tttccggttc
     2101 cggtcgcgcc cgtgataagg accaggccgt tgggtaattc caccagtttt ttcaggaagg
     2161 acggaagccc gatttgttca aacgttggcg gatcaatcgg gttcaagcga aacgctccac
     2221 tcaagtggtt ccgctgaaag aaaaaattga ttcggaaccg gtgcgtaaaa tcgggaagtg
     2281 atacggaaca gtcgatttga cgtttttcgg caaggtgttt ccgctggcct tccttcagaa
     2341 acatgttgac acaatctgtc attacgatat cagtgacagg agggaagcct ttaaagggag
     2401 atagtcttcc atcaatcctg aaaatcggat gactgcccac ttggaaatga acatcggagg
     2461 cgcgatggtt aacggccgct tttaaaacgc caaggatggc cccctggttt ttgatcggaa
     2521 gactgggttt ttgaaccggg ccgcttgccg tatcgcccaa aatataaaga ggttcatcac
     2581 tcacaatgtg ctcctttcgt tttatcaatc caggttagat ggaagaaaag aatgccaaaa
     2641 acgttggctg tgcgactctc tcgcgataag catattgtca tggtcgccgg ctgttccaat
     2701 cggtgaaggg ggtggcgggg ttgaggatcg gggcataatc tttaggaatg ggtaccggaa
     2761 acgtgacgat ttcgtaaatg ccgcccgcaa ttcgaaccaa catcatggca aagcccttcg
     2821 cgagtcctcc gccgatgctc tgcgcaggtt catggttctt gctcatgagc ataggctgga
     2881 tgtaaagttc aaagggtgat gtaaggatgt tggagaaccc ccggccaaat ttcgtaaaag
     2941 cgtcttcttt tgtttcgttg gcaaatccgc tggccgtaaa tcccgccacc agcgcgatcc
     3001 ctaaaaaaag cacaagagca ttttttctca cgataccacc ttccattatt gcctttgtat
     3061 cggccttttc ccggggattc ttcagtgttg gtccaggcac gcgattttat cacgaggtaa
     3121 agacttcgtt cgaaagtccc gacaatatgg atacaatgtc gccgggaagg agggagtatg
     3181 cggtgttttt tgatgatcgt agcgatcctg gccataatag tcttggctgg ggggtggtgg
     3241 gtttggggaa atcttcctga gatcgcgggc tggtggattg agcaaaacag tcgtggagca
     3301 ctggacggtc ctgttgaaat aggagacatc cagtgggttg gatctggtgt tcgtctagag
     3361 aagattgctg gaaaactcag gacccggcag gggccggcgc cttttgtggc gcgttctgtc
     3421 gcctcggtcg atccctt
//
LOCUS       DAOSIO010000178         3437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_24002_length_3437_cov_3.351271, whole genome shotgun sequence.
ACCESSION   DAOSIO010000178 DAOSIO010000000
VERSION     DAOSIO010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24002_length_3437_cov_3.351271"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..1000
                     /locus_tag="PK590_06645"
     CDS             272..1000
                     /locus_tag="PK590_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24310.1"
                     /translation="MAKFHYSTREIAGVSVFDLKGEPAFETVQDVSWKIQKSIRRHRL
                     QRIILNFQRSGTLDAIGLRRILTVCLRPKQSVIYGASEDIVQCIGETYLPSKVDICND
                     EKSVAESFGSFLFDKSVDKKIPARETLRPQDSIGYQFEQRRSHRMHVAIPLEITLMPF
                     QGQKITTRAIATNISEGGLFAEYLDLETAYTINALDSVEGVPVEMYVFPSANFPDEYH
                     LKGMIRRKELRKEQLGLGIEFVDG"
     gene            1315..1674
                     /locus_tag="PK590_06650"
     CDS             1315..1674
                     /locus_tag="PK590_06650"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOG24311.1"
                     /translation="MKQDKDIRFTEKKVDSSWKEHIVASQSTDPEPSQQTQKVATSKI
                     FLSLVQSLGIQALMHLGAIPNPMTNETEVNHEAAKEAIDLLMTLRDKTSGNLSPEEQK
                     ILDSLLSELQIKFSQSV"
     gene            1671..2690
                     /locus_tag="PK590_06655"
     CDS             1671..2690
                     /locus_tag="PK590_06655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOG24312.1"
                     /translation="MTGFKGKALRFIVSVLAICLVVYSVRGKITDSLSILRDETQWTW
                     FVAALVAYLVALGVIAVRMQWVFKVQDVRLNFKECYYLGFVGMFYNLFLPSSVGGDIA
                     RAFYAYKQSGKKIESATSVLLDRLMGFVAIIIIAFIGLIMSRADSQSAYVDYCVYGAL
                     GVLLFCIAFFSSRRFAGLFKGFSIFAPGQKWKERVAEVYNAIYGYRHHQKSVLGAIFL
                     SFIGQSIFILSYFLIAQSLGGGIPFWKFFILIPVVTIVSMAPSIGGLGVREASTLYLF
                     SRYLSPERALAYTLLVDMLIYSFSIGSGVLYAFRGGLKRKGMREMEMMRPERITEGND
                     PEGQI"
     gene            2693..3169
                     /gene="aroQ"
                     /locus_tag="PK590_06660"
     CDS             2693..3169
                     /gene="aroQ"
                     /locus_tag="PK590_06660"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOG24313.1"
                     /translation="MKILVVHGPNLQLLGKREVSVYGTVTLNEINESIRALAKELKIQ
                     IEIAQSNHEGEIVDIIGNSRAAFQGILINPAAYTHTSVAIRDAIAACGIPAVEVHLSH
                     IYKREDFRQHSMTAPACVAQVTGFGKDSYLLGLRGLVSYCSHQCETPKAKSARKKK"
     gene            3166..>3437
                     /locus_tag="PK590_06665"
     CDS             3166..>3437
                     /locus_tag="PK590_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24314.1"
                     /translation="MNVKEIRELLELMAEHQIGEVEIEKENMKIKLRKLSGDRIFMEH
                     APVLPAVLPPVPPVVAAGTVAAPASAASLVVEEGIVIVRSPMVGTF"
BASE COUNT          830 a          728 c          949 g          930 t
ORIGIN      
        1 tataaaatat tgataataag ttgacaaata gatttaaatg atatatagtt ttgtcaggat
       61 atcggacgaa gctttttagc ctggattgtg gctctgaaat aggaggacag gataatgacg
      121 gatgaggaaa ggggagtaac actaagcggg aaaggggggc ctcacatcct tccttgggtg
      181 attcggggtg aatttttttg cgggtacatg tatcccgaac tccataaaaa tctgaggagg
      241 gctgaccgtt cgcggaagag gaaaatttga catggccaag tttcactata gcacgagaga
      301 aatagcggga gtttcggtgt ttgacctgaa aggggagccg gcttttgaga cggtgcagga
      361 tgtgtcctgg aaaatccaga agagtatccg ccgtcaccga ttgcaaagaa ttattttgaa
      421 ctttcagcgg tcgggaacgt tagacgcgat tgggctaagg aggattttaa cggtctgttt
      481 acggcccaag caaagcgtga tttacggggc gtcggaggat attgtgcagt gtattggaga
      541 aacctatttg ccgtcgaaag tggatatttg caacgatgaa aagtctgtgg cggaaagttt
      601 cggatcgttc ttgtttgata aatctgtcga caagaaaatt cccgccaggg aaacgctccg
      661 gccacaggat tctatcggtt accagtttga acaacggcgt agccatcgaa tgcacgttgc
      721 gattccgctc gaaattactc tgatgccttt tcagggtcag aagatcacga cccgggcaat
      781 cgcgactaac attagcgagg gtgggctttt tgcggaatat cttgacttgg aaacagcgta
      841 caccattaac gcgcttgatt ccgtggaggg tgtgcccgtc gagatgtatg ttttcccgag
      901 cgctaacttt ccggatgaat atcatctgaa ggggatgatt cggcgcaagg aattgcgaaa
      961 agagcagtta gggcttggga tcgagtttgt ggacggttga ttcggcggat caatggattt
     1021 ggcgattcgc cggtcaccgt gggggatttt ttttaggggc aaaactgtag tttggaaagg
     1081 tcgtttttca ccgtgactgt cccgttctct tgccctgtgg tattggttaa cctcgtaata
     1141 atcccgctaa aataacccgg ttaaaattcc atagcaaaag attgacggat gggtgttttt
     1201 gaatagaata ttcgccgtaa gagacttacg ggtctcgact cacctgcaga ggttcctggc
     1261 caccaagatc cccactcggt caggaacctc ttttttttag agaggatggt actcatgaaa
     1321 caggacaagg acattcgttt tactgaaaaa aaggttgata gtagttggaa agagcatatt
     1381 gtcgcgtctc agtcgaccga cccggaacct tctcagcaaa cccagaaagt tgccacgtcc
     1441 aagatttttc tcagccttgt ccaatccctt ggcattcagg cgcttatgca tcttggggca
     1501 atcccaaatc cgatgacgaa tgagacggaa gtgaatcatg aggcggccaa ggaggccatt
     1561 gatttattaa tgaccttgcg tgataaaacc agcggcaact tgagtccgga agaacaaaag
     1621 attttagaca gcctgctttc cgagctccaa atcaaatttt cccaatccgt atgacgggct
     1681 ttaagggaaa ggccctgcgg tttatcgtca gtgttctcgc gatctgtctg gttgtttact
     1741 ccgtgcgggg aaagataacg gattcgctat ctatccttcg cgatgaaacc caatggacat
     1801 ggtttgttgc cgcgctagtc gcttacctgg tggccctcgg ggtcatcgcg gttcggatgc
     1861 agtgggtgtt caaggtccag gatgttcgcc tgaatttcaa agaatgttat tacctcgggt
     1921 ttgtcggcat gttctataat ctctttttgc cgtcctcggt tggcggggat attgcccgcg
     1981 ccttttacgc gtacaaacag tcaggtaaaa agattgaatc agcgacatcg gttttactcg
     2041 atcgtctcat gggatttgtc gcgattatta tcattgcctt tatcggattg attatgtcac
     2101 gcgccgattc ccagagcgct tacgttgact actgtgttta tggggcgctg ggagttctcc
     2161 ttttctgtat cgctttcttt tcgagccgaa gatttgcggg tttgtttaaa gggttttcta
     2221 tttttgcccc ggggcagaaa tggaaagaga gggtggcgga agtttacaac gcgatttatg
     2281 gataccgtca ccatcaaaag tcggttctgg gggctatttt tctttctttt atcgggcagt
     2341 ccatatttat cttgtcatat tttctgattg cccaaagctt gggcggcggg attccttttt
     2401 ggaaattttt tatcttgata cccgtggtga ccatcgttag tatggcacca tcaattggcg
     2461 gattgggagt gcgtgaggcc agcacgcttt atctttttag ccgctacttg tcgcccgaac
     2521 gggctttggc ttatacgttg ctggtggata tgctcatcta cagttttagc atcggttcgg
     2581 gcgttctcta cgcgtttcgc gggggattga agcgaaaagg gatgcgtgaa atggaaatga
     2641 tgcgccccga gcggatcacg gagggcaatg atcccgaggg acagatctag acatgaaaat
     2701 acttgtggtc catggaccta acttgcaact tttgggaaaa cgagaggttt cggtgtacgg
     2761 taccgttaca ctgaacgaga ttaacgagtc cattcgggcg ctcgcgaaag aattgaagat
     2821 tcagattgag atcgcccaga gtaatcacga aggagaaatt gttgatatca tcgggaattc
     2881 ccgcgctgct tttcagggga ttctgatcaa tcccgccgcg tacacgcata ccagtgtcgc
     2941 cattcgggat gctattgcgg cgtgcggcat tccggcggtt gaagttcatc tcagtcatat
     3001 ttacaaacgc gaagatttca ggcagcactc catgacggcg cctgcctgcg tggcgcaggt
     3061 cacaggattc gggaaagaca gttatcttct gggactccgg ggattggtgt cctattgttc
     3121 tcatcaatgc gaaaccccta aagccaaatc ggcgaggaag aaaaaatgaa cgtaaaagag
     3181 atcagggagt tgctggagct tatggccgaa caccagatcg gcgaggttga gattgaaaaa
     3241 gaaaacatga aaatcaaact acgcaaattg tcaggggaca ggatttttat ggaacatgcc
     3301 ccagtgttgc ccgcggtgct tcctccggtg cctcccgtgg tcgcggccgg gaccgtcgcc
     3361 gcgcccgctt ccgcggcttc tctggtggtg gaggaaggga ttgtgatcgt ccgttccccg
     3421 atggtgggaa cgtttta
//
LOCUS       DAOSIO010000179         3384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_24480_length_3384_cov_3.491138, whole genome shotgun sequence.
ACCESSION   DAOSIO010000179 DAOSIO010000000
VERSION     DAOSIO010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24480_length_3384_cov_3.491138"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            465..962
                     /locus_tag="PK590_06670"
     CDS             465..962
                     /locus_tag="PK590_06670"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG24315.1"
                     /translation="MSPAVKIDERKETMKATIQTTLGTIEVELYADKAPKTVENFATL
                     ARKGFYDGIIFHRVIPGFMIQTGDPTGTGTGGPGYKFADEFNKSLRHDKPGVLSMANS
                     GPNTNGSQFFICEVPTPWLDDRHSVFGQVVSGMDVVTKIANAKRDSRDKPLETIQMQK
                     VTILE"
     gene            1327..2793
                     /locus_tag="PK590_06675"
     CDS             1327..2793
                     /locus_tag="PK590_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOG24316.1"
                     /translation="MWSFEAILLWAAVLLFASALSSKISDKFAVPVLLLFLAIGMLSG
                     SEGIGKIYFDNAQQAKNIGVLALIFIIFSGGLDTNWKDTKPIVIPGALLSTVGVFITA
                     IITGLCAAYILKISILEGILLGSIVSSTDAAAVFAILRSKRISLKKPLKPLLEFESGS
                     NDPMAIFLTTGFLGLLTAKSTEIVSLIPRVFLDMGMGALIGYAMAKGIVFLIRRIKLD
                     YEGLYPVVTISLVLFTYVIAIFLKGNGILAVYLAGLGLGRAEFPNKKSIMKFHDGLAW
                     ISQIAMFVTLGLLVYPSHLLPLIGAGLLVTLILMLIARPVSVLLCLLPFNFSMPKKIM
                     LSWVGLRGSVPIILATFPLAAGIDQAQTIFNVVFFIVIASVFVQGTSIPMVSRLLKLD
                     VPLPNRKTYPLEFEKVEGVDAELTDVVVPYNSAVVGKMIKELNVPEKCLIMLISRNGK
                     FAIPSGLTVIEGGDILLMLASDADLAAFQEKLANFKKE"
BASE COUNT          810 a          714 c          916 g          944 t
ORIGIN      
        1 gaagaaagtt atctggatca tcgtggttct ggttctgccg cttgttggca tgatccttta
       61 ttttttgttg ggccgggaga aaaaagcagc gtaagaacgt acgagtttaa gccctcgcgt
      121 ttttcttttt tttctgagaa aaacgcgggg gttttttatt ttatccatac tgttttccga
      181 tgccgctgtg ttttggctaa tggcgaaacc cgggcacggt tccaaatctc gaaggtattt
      241 ttaatttgaa aacgttttag gtttgaaaca tttcagggat gtgtcatact cctataaatt
      301 ttttaacgga ggattccagt atgaagctta aagtgatttc actggtgatg agtgccgctt
      361 tgatctcttt attccagggc tgtgcttctc agcctaaaac gccaacgttc gcggagaaag
      421 cttcggccca gaccgcgacc caaacgatct tgacgcaagc tgagatgtct ccggctgtga
      481 agatcgatga aagaaaggaa accatgaagg caacgataca aacgacgctg ggaacgatag
      541 aagttgaact ctacgcggac aaggcgccta aaacggtaga aaatttcgcg acgctggcga
      601 gaaaagggtt ttatgacgga atcatttttc acagggtgat tcccggcttc atgatccaaa
      661 cgggggaccc aacggggacc ggaacaggcg ggcctggata caagttcgcg gatgaattta
      721 acaaaagtct ccggcatgat aagccggggg ttctctcaat ggcgaactcg ggtcccaata
      781 cgaatggaag ccagttcttt atctgcgagg tcccgacgcc ctggctcgat gacagacatt
      841 ccgtatttgg gcaggtcgtg agcggcatgg atgtggtgac gaaaatcgcg aacgcgaaga
      901 gggattcgcg tgacaagccg cttgaaacga tccagatgca aaaggttacg attctcgaat
      961 agtaaaaatc gggggtggtt ttagaacgaa tcgcatccgg atcaaaatcc tcttcaaaga
     1021 taatcctggc tcgacataga ttcgggtcgc gctggcacca cgcgttagct tcttttctga
     1081 gtggcttaaa agccgcattt ccgaggtgtg tcactatggg cggagaatcg ggagaaaacg
     1141 tgaaattttg cgtttgctcc ggtgtgctaa ccggtgtagt gtgatgtcgc aatttgataa
     1201 aagtctcgcg gtaaaggtgg attttaaaat gacaagcgaa aattctgtcg caaaatcttt
     1261 ttaacaagat atgggaaaaa ctggcgacga attgaaatag acggctaaca aggaaacttt
     1321 ttagaaatgt ggtcatttga agctattttg ttgtgggcgg cggtattgct gttcgcgagc
     1381 gctctttcga gcaagatctc ggacaagttc gcggtgccgg ttttgttgct ttttttggcg
     1441 attggaatgc tttctgggtc cgaggggatc ggcaaaatat attttgataa cgcccaacag
     1501 gctaaaaata tcggtgtttt ggcgcttatc tttatcatct tttcgggcgg gcttgatacc
     1561 aactggaaag acacaaaacc gattgttatc cctggggccc tgctttcaac tgtcggggtg
     1621 tttattacgg cgattataac ggggctgtgc gccgcttata tccttaaaat ttctatcctt
     1681 gagggaatat tacttggttc tatcgtttcg tccacggatg ccgccgcggt atttgctatt
     1741 ttgaggtcaa aacgaatcag cctgaagaag ccgctcaagc cgctcctgga atttgagtcg
     1801 ggtagcaacg acccgatggc tatcttcctg accacgggtt tcttaggttt gctgacagca
     1861 aaaagcacgg aaatcgtttc gctcatccca agggtctttc tggatatggg gatgggggcg
     1921 ctgattggtt atgccatggc gaagggaatc gtttttttga tcaggcggat taaactggat
     1981 tatgaggggc tttatccggt cgtaacgatt tcgcttgtat tgtttactta tgtcatcgct
     2041 attttcttga aaggcaacgg gatcctcgcg gtttacctcg cgggacttgg gctggggcgg
     2101 gcggaatttc ccaacaagaa atctatcatg aaatttcacg acggcttggc ctggatcagc
     2161 cagatcgcaa tgttcgtgac tttggggtta cttgtttatc cgtctcatct gcttccgctg
     2221 attggagcgg gactcttggt tacgcttatt ctcatgctca ttgcccgccc ggtgagtgtt
     2281 ctcttgtgtt tactgccgtt taatttttcg atgccgaaaa agattatgct ttcgtgggtg
     2341 ggtttaagag gttcggtgcc gattattctg gccacgttcc ctctggcggc tggcatagac
     2401 caggctcaaa ccatttttaa tgtcgtgttt ttcattgtca tcgcgtctgt ttttgttcag
     2461 gggacatcaa tccccatggt ttcgagattg ctcaagctgg acgtgccttt gcccaacagg
     2521 aagacgtacc cgttagaatt tgagaaggtc gaaggcgttg acgcggagtt gacggatgtc
     2581 gtcgttccgt ataattccgc agtggtaggc aagatgatca aggaattgaa tgttcccgag
     2641 aaatgtttga tcatgcttat ttcacgtaat ggcaaatttg ctattccgtc cggactcacc
     2701 gtgattgaag gaggagatat tttgctgatg cttgccagcg atgcggattt ggcggccttt
     2761 caggaaaagc tggcgaattt caagaaagaa taaattccga agacgcgtct tttgacttgc
     2821 cttccgtcgc cacgtttcgt cagaatggac atttcacgtg gtcttccgtt cggcccctgg
     2881 atcgccggaa taacttcagc tgtggcgggc gattctttaa cgtcgtgagt attagcggag
     2941 ctcaagctat atttcccgcg gagttgaaaa aaatgagtgc ccccggtctt tttttgtgta
     3001 taatccacga tgttgttctt tcaaattaac aggtgacaga cattggccag gcttttaaac
     3061 ggccaagttg ggaagagaga tcaccgaggt ttgggtgagg ctccacggtg ccgccgctgt
     3121 agttcttaat aggcaataac ccactgtttc gaggatatcc aggaaatggg aagggtgcca
     3181 accccgcgaa atttcaacga tggaacggtt gaaattcagg cgagcggggt gaacgagtca
     3241 gaatatcaac ctgtcgctga gaaaactaag gcgtgaaagc gtacggagta cggcgtagaa
     3301 attttcgccg cacgcggaac gcgtcgcgcc tttcgaaaaa ttgcctccgc gcagaggacg
     3361 agacaggttt tatcccgttc ggga
//
LOCUS       DAOSIO010000180         3316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_25120_length_3316_cov_3.993867, whole genome shotgun sequence.
ACCESSION   DAOSIO010000180 DAOSIO010000000
VERSION     DAOSIO010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25120_length_3316_cov_3.993867"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PK590_06680"
     CDS             complement(<1..530)
                     /locus_tag="PK590_06680"
                     /inference="COORDINATES: protein motif:HMM:NF014873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24317.1"
                     /translation="MEYAGNDTNTQVAPVSGSQGVVLGKVISVRGPVVDVRFERVNDL
                     PALYDVLIGQSLNKKKVVMEVSEHLSKNDVRCIALSETLNLQLNSKVVNTQKPVSVRT
                     GDELYGRIINVFGDPVDNMPDFSGTEFKVTRRPPRLEQYDLKSKFSAKPEFLETGIKY
                     LDVLFPLCKGSKMGILG"
     gene            complement(539..1444)
                     /locus_tag="PK590_06685"
     CDS             complement(539..1444)
                     /locus_tag="PK590_06685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HOG24318.1"
                     /translation="MGKVTRIMGDMDDVDALLEILSVIKDVAMNRFFAFAQRKANIQS
                     FFDEFLSFFKLVQDVETDCPLLRNDNPGVLILTIASDMAFMSQLNSRVASLTIQQAQK
                     YPGSTVTVLGRRCAERLKILELKNIDRVFVADEFSDRYALALAIRDHLVGQVLSGKVG
                     KVLIVYLWAKSFSIIKPRILTLLPASELVRTGGGDLTEGEGASQEDETSADSEDKTNF
                     FMEAEPAQMMCALADIWIHSRLFEILSDILLVEFAVQAQQLESALESLGSEKKSLLVS
                     FRKAGRDELNKAMREVFTQTSVSRR"
     gene            complement(1472..2200)
                     /locus_tag="PK590_06690"
     CDS             complement(1472..2200)
                     /locus_tag="PK590_06690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOG24319.1"
                     /translation="MWIFGFVLIQIIVFGAVLYFYKKITAGETENTVQRLGAVYEDLI
                     KKQKDLSEKLEAAEQEYQAKKEEAAQIADKLANQAMDETRKKEEVILKKARAEAEDIL
                     AKAHASKDQLSKELEVEANRKVVDFSAELLKQIHDEQLKILLHKHFIKSFLERAKKSD
                     LASVELHGQKPVLRTPILLEKEDKDQLQRVLSEQLKIPGLQIDEVADETLVAGVALQV
                     GTLVLDGSFANAIREAADAYKEEV"
     gene            complement(2215..2433)
                     /locus_tag="PK590_06695"
     CDS             complement(2215..2433)
                     /locus_tag="PK590_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOG24320.1"
                     /translation="MQNLTVIIIMFIAVIGPSIVIAVVGAATIQALGRNPSAAPKIYL
                     GVILMLLFAEAASIISLLVVFQIFVPRT"
     gene            complement(2452..2730)
                     /locus_tag="PK590_06700"
     CDS             complement(2452..2730)
                     /locus_tag="PK590_06700"
                     /inference="COORDINATES: protein motif:HMM:NF014838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24321.1"
                     /translation="MKLKFHLKVMSLKGVAIDEEVESVYLTGDDGEFELLPFHHPLLA
                     SLPEGELKIAYHESIPIKAGVLSFKDNECRVIAEIDPDFKNYKQVWDI"
     gene            complement(2734..>3316)
                     /locus_tag="PK590_06705"
     CDS             complement(2734..>3316)
                     /locus_tag="PK590_06705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012527.2"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOG24322.1"
                     /translation="DLTAYVPTNLVSMTDGQLFLNASLFNEGFRPAVDVGLSVSRVGS
                     KVQWKATKEVSKSLRMEFIQFKELLRASKLQASGQSEETRENLRAGKVLQALLTQPAN
                     KPVSIEIQIMSFFAKENQLFKDLELPHMQEFLTGFEPYAKESDPSLFSLIREKQVLDD
                     EIKKRMTRILEEYIGTLKKKFQVEDEGAVVETA"
BASE COUNT          883 a          918 c          644 g          871 t
ORIGIN      
        1 ccgagaattc ccattttgct tcctttacat aaggggaaaa ggacatcgag atatttgatg
       61 ccggtttcca aaaattcggg cttcgctgaa aatttactct tgaggtcata ttgctccagc
      121 cggggcggcc gccgcgtcac cttgaactct gtacctgaaa aatcgggcat attgtccact
      181 ggatctccaa atacattgat aatacggcca tagagttcgt cccccgtccg gacggaaaca
      241 ggtttttggg tgttcacaac ttttgaattg agctgaagat taagagtctc agagagcgcg
      301 atacaccgga catcgttctt gcttagatgt tcggacacct ccatcacaac ctttttcttg
      361 ttaagtgact ggccgatcaa aacgtcgtac aaagccggca aatcattcac acgctcaaac
      421 cgaacgtcaa ctaccgggcc tcggaccgaa atcacttttc ccaaaacgac accctgagaa
      481 ccggaaaccg gggctacctg agtattcgta tcgttccccg catactccat tggttttatc
      541 acctcctcga aacgctcgtt tgggtaaaca cttctctcat agccttgttc aattcatccc
      601 tgcctgcctt cctaaaactg accagcaagg acttcttttc ggagccaaga gattccagag
      661 cagattccag ctgttgcgcc tgcaccgcaa attcgacgag aagtatgtca gataatattt
      721 cgaaaagcct tgaatggatc cagatatcgg caagcgcgca catcatctgg gctggctcgg
      781 cttccataaa aaaatttgtt ttatcttcgg aatcagccga tgtctcatct tcttgtgatg
      841 caccctcccc ctccgtcaaa tctcctccgc cggttctaac caactccgat gcgggaagga
      901 gcgtcaaaat acgaggcttg ataatactaa aagatttggc ccataagtag acgatcaaaa
      961 cctttccgac cttaccactc aaaacctgtc caaccagatg atcgcgtatc gcgagcgcta
     1021 acgcgtagcg atcggagaat tcatccgcca caaaaacacg atcgatgttt ttcagctcca
     1081 gtatcttcaa cctctccgca caacgacgtc ccaaaaccgt caccgttgat cccggatatt
     1141 tctgagcctg ctggatagtt aaactcgcca cccggctatt aagctgggac ataaaggcca
     1201 tgtcggacgc aatcgtcaaa attaataccc cgggattgtc attcctgaga agaggacaat
     1261 ccgtctcaac atcctggaca agtttaaaaa aactaagaaa ctcatcaaag aaactctgaa
     1321 tattcgcttt ccgctgggca aacgcaaaaa agcggttcat cgcaacgtct ttgatcaccg
     1381 aaagaatctc caggagggcg tccacatcgt ccatgtcccc cataattctt gtgaccttac
     1441 ccacaacgat cctcctcctg ttctctcatc gctacacttc ttccttatac gcgtccgccg
     1501 cttctctgat ggcattcgca aaactgccgt ctaggaccag ggttccgacc tgtaacgcaa
     1561 ctccggcaac aagagtctca tcggcaacct catctatctg aagtccagga attttgagct
     1621 gttcggaaag aacccgctga agctgatctt tatcttcttt ctccagaaga atcggggttc
     1681 ggagtacagg cttctgtcca tgcagttcca cggaggctaa atccgatttt tttgcccgct
     1741 ccaaaaaact ctttatgaag tgcttatgaa gcaatatttt taattgttca tcgtgaatct
     1801 gttttaagag ctccgcggaa aaatcaacaa cttttcgatt ggcctccacc tccagttcct
     1861 tgctgagctg atccttggac gcatgagcct tggcgagaat atcttccgcc tccgcacgcg
     1921 cttttttgag aattacctcc tccttcttcc gggtttcgtc catggcctga ttggctaatt
     1981 tatccgcaat ctgggccgcc tcctccttct tggcctgata ttcctgttcc gcggcctcca
     2041 gtttctcgga aaggtctttc tgtttcttaa tgagatcttc ataaaccgct ccaagtctct
     2101 gaacggtatt ttcagtctct cccgccgtta tttttttata aaaataaagg accgcaccaa
     2161 aaacaattat ttgaattaga acaaatccaa aaatccacat agtctttctc cttatcaagt
     2221 ccgtggaaca aaaatctgaa aaactaccag taacgaaata atcgacgccg cttccgcgaa
     2281 caaaagcatc aggatcacgc ccaaataaat cttaggcgcc gcggaaggat ttcgtcccaa
     2341 agcctggata gtggccgcgc caactaccgc gatcacgatc gacggcccga tcaccgcgat
     2401 aaacatgata ataatcactg taagattctg catcgcccag caccctctcg tctaaatatc
     2461 ccaaacctgt ttatagtttt taaaatccgg atcaatttcg gcaatcacac gacattcatt
     2521 atccttaaag ctcaaaactc cggccttgat ggggatactc tcgtgatatg cgattttcaa
     2581 ttccccctcc ggtagcgatg ccagaagagg atgatgaaac ggaaggagtt caaattcccc
     2641 atcatccccc gtcagataaa ccgactccac ttcttcatca atcgcaactc ccttcaggct
     2701 cataactttt aaatgaaatt tgagtttcat acctcaggcc gtctcaacca cggctccttc
     2761 gtcctcgacc tgaaacttct ttttcaaagt tccgatatat tcttcaagaa tccttgtcat
     2821 ccgcttcttg atttcatcgt ccaaaacctg cttttccctt atcagggaaa aaagagacgg
     2881 atccgattct ttcgcatagg gttcaaaacc cgttaaaaat tcctgcatat gaggcagctc
     2941 aagatccttg aaaagttggt tctccttggc gaaaaatgac atgatctgga tttcgatcga
     3001 aacaggcttg ttggctggct gggtcagcag agcctgtaaa actttcccgg ccctcaaatt
     3061 ttcccgcgtc tcttccgatt ggcccgatgc ctgaagcttg ctggcgcgta ataactcttt
     3121 aaattgaata aattccatgc gcaacgactt agaaacttcc ttggtagcct tccactgaac
     3181 cttgctcccg acacgtgaaa cagaaagccc gacatccacc gctggccgaa aaccttcatt
     3241 aaacagggag gcattcaaaa aaagctggcc gtccgtcatc gaaacaagat tcgtaggtac
     3301 ataggccgtc agatcg
//
LOCUS       DAOSIO010000181         3262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_25689_length_3262_cov_3.651076, whole genome shotgun sequence.
ACCESSION   DAOSIO010000181 DAOSIO010000000
VERSION     DAOSIO010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25689_length_3262_cov_3.651076"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PK590_06710"
     CDS             <1..439
                     /locus_tag="PK590_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24323.1"
                     /translation="SILEAIAERGELGPNDEAVIRQSAIGLARVHRAGFSLIDTQPVN
                     CIWVPEEQKVYFTDLEFCSRQDKRSWDVGFFLCYLAARLPENLLKTIRSLFLQSYRQE
                     RQIDLAEIDDTSRELIEYLPIFRTILDIRRFTPEELFEGLITT"
     gene            complement(451..894)
                     /locus_tag="PK590_06715"
     CDS             complement(451..894)
                     /locus_tag="PK590_06715"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOG24324.1"
                     /translation="MRIRLSLAWVKTGSSVSRSFKSSPAYLLLQDYVMRISRFFPCEA
                     GEYSPKARSLVWVCERDKKSKPLSSNDLAKELEKVLNSGIQELHVVIGDADGFSAKEL
                     ETMKPALKWSFGPLTLPHELAAVVAAEQIYRAITILRNHPYHSGH"
     gene            993..2099
                     /locus_tag="PK590_06720"
     CDS             993..2099
                     /locus_tag="PK590_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24325.1"
                     /translation="MTSRIEHIYIDLGVKTHPLTRRIIRALPGTRVTLVRNRKTFLRE
                     SEKIPLSLGKRMLWLSQFKGPLLKPCPGTGEDYLCCRYYTINAQTNCPLDCSYCILQN
                     FLNFPLLTVYVNVSDVFKEIDALIRSEPGRLFRMGTGELTDSLALDPLTKMSEGLIRH
                     ALKRRMILEIKTKTDFIDHLPSIPKHNVLISWSLNPQEIVRGEEHKAAPLKSRLKAAK
                     AAIKKGYRLAFHFDPLLIFKGWETKYAKLITQLTREIPEKEVMWISMGSFRYPPPLRR
                     ILDKRFPKSKILSGEFIKGLDGKMRYFRPDRTPLYRTVHEMIRKKWTDVFVYFCMENS
                     TVWNDVMGASPRNNEHLDQMFQESIERRFDGFKC"
     gene            complement(2089..2544)
                     /locus_tag="PK590_06725"
     CDS             complement(2089..2544)
                     /locus_tag="PK590_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOG24326.1"
                     /translation="MNLKTKGFTLIEIMIVVAIIGLLVAIMIPNFLRMRINTNESLIR
                     GELRTFSTANESYRAAQAVPVYAADIATLHAGQYLDERWVSGGKYGYSFKYVAAANGE
                     TYGVEASPVSSGASGINYYCVDQTGVIRKSPTPGISGETGCTGGVSINT"
     gene            complement(2673..3089)
                     /locus_tag="PK590_06730"
     CDS             complement(2673..3089)
                     /locus_tag="PK590_06730"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HOG24327.1"
                     /translation="MRRSLFMSAIIALSVIAMVVPSVFAGSYDLKEITPAVKQALENR
                     QARYSQLQSLKQKGAVGEDNKGFVADLGRDPQAAAMVSSENRDRRIIYQALVDQNKLG
                     SAGMTEVQKVFAEVQRDKASSGEYIQTPAGEWSRKT"
BASE COUNT          828 a          916 c          750 g          768 t
ORIGIN      
        1 ctcgatactg gaggccatcg cggaacgggg cgaacttggg cccaatgacg aggccgtcat
       61 ccgtcaatcc gctatcggct tggccagggt ccaccgcgcc ggattttcat tgatcgacac
      121 ccaacccgtc aactgcatct gggtaccgga agaacaaaaa gtttatttca ctgaccttga
      181 attttgcagt cggcaggaca aacgctcctg ggacgttggg ttttttctat gttacctcgc
      241 cgcgcggctt ccggaaaatc tcctcaaaac gatccgttct ctctttcttc aaagttaccg
      301 gcaagaacgc caaatcgacc tggctgaaat agacgacacc agccgcgagc tcatcgagta
      361 ccttcccatc ttccggacca tccttgatat cagaagattc actcccgaag aattatttga
      421 ggggttgatc acaacttaaa aaatctgatt ctaatgcccc gaatggtagg gatgattacg
      481 caggatggtg atcgcgcggt aaatctgctc ggcggccaca accgcggcaa gctcgtgcgg
      541 aagcgtcaac ggaccaaaac tccacttgag cgcaggcttc atcgtctcaa gttccttcgc
      601 cgaaaatccg tctgcgtccc caatcacgac atgaagctcc tgaattccgg agttaagaac
      661 cttctccaat tctttggcga gatcatttga tgagagcggc ttggattttt tgtcccgctc
      721 acaaacccag accagggatc gggccttggg tgaatactct cctgcctcgc acgggaaaaa
      781 ccgggaaatt cgcatcacat aatcttgcag taaaagatac gccggggaac ttttaaatga
      841 cctgctgact gaactgccgg tcttgaccca tgccagtgat aatcgaatcc gcatcaggac
      901 acctccctca agtctcattc tagccaggga aatatttagt gccaggcgtt ttttgcttct
      961 cgggaggctt ttgccccgtt aaaatgccgt tcatgactag caggattgaa catatttaca
     1021 ttgatttggg tgtaaaaacc caccccctca cccggcggat tatccgggct ctcccgggaa
     1081 ctcgcgtcac tcttgtgagg aaccggaaaa cattcttgag agagtcggag aaaattccgc
     1141 taagcctcgg aaaacgcatg ctctggctga gtcaattcaa agggccattg cttaaaccct
     1201 gccccggcac cggagaagat tacctctgtt gccgctatta tacgattaac gcgcaaacaa
     1261 actgtccgct cgactgttcc tactgcatcc ttcagaactt tctcaatttt cccctgctga
     1321 ccgtttatgt taacgtcagc gacgtattca aagagattga cgcgctcatc cggtccgaac
     1381 ccggacgcct tttcaggatg gggacgggag aactgacgga cagcctcgcc ctggacccct
     1441 tgacgaaaat gagcgaaggg ctgattcgcc acgcgctgaa gcgaaggatg attctcgaaa
     1501 taaaaacaaa aaccgatttc atcgatcatt taccctccat ccccaaacac aatgtcctga
     1561 tatcgtggtc gctcaacccc caggagattg tccgcgggga agaacacaag gccgcgcccc
     1621 tcaagtcgcg cctcaaggcc gcgaaagcgg cgattaagaa aggataccgg ctcgcctttc
     1681 attttgatcc cctgttgatt tttaaaggct gggaaacaaa atacgcgaaa ttgatcactc
     1741 agctgacccg ggaaataccg gaaaaggaag tgatgtggat cagtatgggg agtttccgtt
     1801 atccccctcc gctaagacgg attttggaca aacgatttcc gaaatccaaa attttaagtg
     1861 gcgaattcat caaaggcctg gacgggaaaa tgcgctattt ccgtcctgat cgcaccccgc
     1921 tttatcgaac cgtccatgaa atgattcgca aaaagtggac tgatgttttt gtctactttt
     1981 gcatggaaaa ctcgaccgtt tggaacgatg taatgggcgc gagcccaagg aataatgagc
     2041 atctggatca gatgtttcag gaaagcatcg agcgcagatt tgacggtttt aagtgttaat
     2101 cgaaactcct ccggtgcaac cggtttcacc agatatcccg ggagtcggtg acttgcgaat
     2161 gactccagtt tgatccacgc agtagtaatt gattcccgag gcgccagaac tcacgggaga
     2221 cgcctccacc ccataagttt ctccgtttgc cgccgccacg tatttaaaac tgtaaccgta
     2281 cttaccgcca ctcacccatc gttcatccag atactgaccc gcgtggaggg tcgcaatatc
     2341 agccgcgtaa acaggaacgg cctgtgccgc ccggtaggat tcgttagcgg tactaaaggt
     2401 ccggagttcg ccccgaatga gggactcgtt tgtattaatc ctcattctca aaaaattcgg
     2461 gatcatgatc gcgaccagca gtccgatgat cgcgacgaca atcataatct cgattaacgt
     2521 aaaccctttg gtttttaaat tcatacattc tccggtttca gacaccccaa cacttcaaac
     2581 aagcggttca caggataaac ccgcccaatc tcgctcacct caaaaaagaa ctcaaccatt
     2641 ccggttatga accgtccgcc agtctgaaca agtcaggtct ttcgcgacca ttctccagct
     2701 ggagtttgga tgtactctcc gctggaagcc ttgtctctct gaacctcggc aaaaactttt
     2761 tggacttccg tcatgcccgc ggaaccaagc ttgttctggt ccacaagcgc ctgatagatg
     2821 atccgccggt cacggttttc cgaactgacc atcgcggcgg cctgcggatc gcgtccgaga
     2881 tcggccacaa aacccttgtt atcctcaccc acagcgcctt tctgtttcag actttgtaac
     2941 tgcgagtagc gtgcctgacg attttcaagg gcctgcttca ccgccggcgt tatttccttc
     3001 aaatcatatg agccggcaaa aaccgaagga accaccatcg caatcacact cagagctata
     3061 atcgccgaca taaaaagaga gcgcctcatc actttcctcc ttgagcaacc agatcctcaa
     3121 tacccgtcgc agtttctttc agaccgcgaa tatccaccac cacgttcgca ttgatggtga
     3181 tgggccgatc ggggtctccc acggccttga cggtacatcc ggccacgagg agcaaactga
     3241 acataatccc tgaaaatttt cg
//
LOCUS       DAOSIO010000182         3231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_25997_length_3231_cov_3.389798, whole genome shotgun sequence.
ACCESSION   DAOSIO010000182 DAOSIO010000000
VERSION     DAOSIO010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25997_length_3231_cov_3.389798"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PK590_06735"
     CDS             <1..635
                     /locus_tag="PK590_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOG24328.1"
                     /translation="AIEILVVDDNSPDGTGKIVAEWSERETRIHLLAREKKMGLGPAY
                     IAGFKWGLERDYELFIEMDADLSHRPRYLADFLKKIKQCDMVVGSRWMKGGRVANWPL
                     SRVLLSSFAGLYCRLILGVHVADMTAGYVSYRRRVLESIPLEDVKADGYCFQIEMKYR
                     VLQKGFKVIETPILFTDRRVGKSKISRSIVYEAFFKVWWLRFNRNRFRMN"
     gene            707..979
                     /locus_tag="PK590_06740"
     CDS             707..979
                     /locus_tag="PK590_06740"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG24329.1"
                     /translation="MAIREDVIYTPKEVQEYLKDSQSTMTRMLKKGLIRSARIGKQYR
                     IMGKEVLRVISPSLEDKVGVAYNKARRWVHDETPFRPVDKSAKYKE"
     gene            976..2148
                     /gene="pseC"
                     /locus_tag="PK590_06745"
     CDS             976..2148
                     /gene="pseC"
                     /locus_tag="PK590_06745"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HOG24330.1"
                     /translation="MTTQTFKKIPYGRQWVGREEIRAVTEVLKSDFLTQGAKVPEFER
                     ALARKVGSDYAVAVANGTAALHLAAIALELKPGDEVITTPISFLATSNAIIYAGARPV
                     FGDIDPETQCIDPRNIESKITKRTRAIFVTDFAGHPADMAVIYDIAGKHKLKIVEDAA
                     HALGSVCGGSRVGSCRYADMTIFSFHPVKHITTGEGGAITTNDSQLREKLRELRTHGV
                     TRDPQKLLNKKQGGWYYEMQSLGFNYRLTDIQAAIGIEQLKKSGRFIERRREIAKKYT
                     RAFSDLPGILTPVERSNMKHVYHLYVIRLQGKFAGKRQIFFEQLHKRGIGAQVHYIPI
                     PEQPYYRSLGYTAEDCPNARSYYASAVSLPMYPGLTNTEVHSIIKAVKDICHRIFR"
     gene            2166..3191
                     /gene="pseB"
                     /locus_tag="PK590_06750"
     CDS             2166..3191
                     /gene="pseB"
                     /locus_tag="PK590_06750"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HOG24331.1"
                     /translation="MKKNDKNLQLNGKTILVTGGTGSFGQRFVRTMLENFKPKKIIVL
                     SRDEMKQWNMQREFAALDKKNVLRFFIGDVRDKGRLHRAFYGVDYVIHAAASKIIPTA
                     EYNPFECIKTNVQGAMNVIEAAIDQKVEKVVALSTDKACNPINLYGATKLCSDKLFVA
                     GNSYVGTRNTLFCVVRYGNVMGSRGSVIPFFRSIAHTGELPITDERMTRFMITLEAGV
                     ELVLRALREGIGGEIFVPKIPSMKVVDVAGTIAPKARLKIVGIRPGEKIHEMMISEED
                     ARTTYEYGAYYKILPAIHDWQYCGKRIGSGKLCPEGFSYDSGTNDEWMSPSDLRKWMD
                     REYGKSA"
BASE COUNT          863 a          661 c          886 g          821 t
ORIGIN      
        1 cggctattga aatacttgtg gtggatgaca actcgccgga tggaacggga aagattgtcg
       61 cggaatggtc ggagagggaa accagaatcc acctgctggc ccgggagaaa aaaatggggt
      121 tgggaccggc ttatatcgcc ggatttaaat ggggtttgga gcgggattat gagctgttta
      181 tcgaaatgga cgcggatcta tcacatcgtc ccagatattt ggcggatttt ttaaaaaaaa
      241 tcaaacagtg tgacatggtt gtcggatcac gttggatgaa aggcggcaga gtcgcgaatt
      301 ggccgctctc ccgtgtgttg ttaagtagtt ttgcggggct atattgtcgg ctcatcttag
      361 gtgtgcatgt tgcggatatg acggctggat atgtctccta ccggaggcgg gttttggagt
      421 ctatccctct cgaagatgta aaggctgacg gttactgctt tcaaatagag atgaagtatc
      481 gtgttcttca gaaagggttt aaggtcattg agacacccat cctgtttact gacagaaggg
      541 tggggaaatc caaaatttcc cgcagcatcg tttacgaggc attttttaag gtttggtggc
      601 tccgcttcaa tcgaaataga ttcagaatga attaaaagaa tttattgaca tctaatgact
      661 tcaaagttat attttgctat attttaaatt aatcaaagag ggaaaaatgg ctattcgcga
      721 agatgttatt tacacaccca aagaggtcca ggagtatctc aaagacagcc agagtacgat
      781 gacccggatg ctgaaaaagg ggcttattcg ttcggcccgg atcggtaagc agtaccggat
      841 tatggggaag gaggtcttgc gtgtaatatc cccgtcactc gaagacaaag taggtgtagc
      901 ttacaacaag gcccggcgat gggtccatga cgaaacgccc ttccgtccag ttgacaaaag
      961 tgcgaaatat aaagaatgac cactcaaact tttaaaaaaa tcccctatgg tcggcagtgg
     1021 gttgggaggg aagaaatccg tgctgtgacg gaggttctca agtcggattt tttgacccaa
     1081 ggggctaagg tgccggaatt tgaaagggca ttagcccgaa aggttggatc ggattacgcc
     1141 gtggcggttg ctaatgggac ggcggcattg catctggccg caatcgcgct tgagctgaag
     1201 cccggtgatg aagtaatcac gactccgatc agctttctcg ccacctcaaa cgcgatcatc
     1261 tatgccggag cccgtcctgt ttttggtgat attgatccgg agacccaatg tatagatccc
     1321 cgaaacattg aatcaaagat cacgaagcga acccgggcaa tttttgtgac tgattttgca
     1381 ggtcaccctg ccgacatggc tgtaatttat gacattgccg gaaagcataa gctcaagatt
     1441 gtggaggatg cagctcacgc cttaggctct gtttgtggtg gctctcgcgt tggttcgtgc
     1501 cgttatgccg atatgacgat tttcagcttt cacccggtca aacatattac gaccggggaa
     1561 gggggtgcta tcacaacgaa cgattcgcaa ctgcgtgaaa aactgcgcga actcaggacg
     1621 cacggtgtga cgcgcgaccc ccaaaaactt ctcaataaaa aacagggggg ctggtactat
     1681 gaaatgcagt cgctcggttt taattatcgt ttgacggaca ttcaggcggc cattggtatt
     1741 gaacaactta aaaaatccgg ccgttttatc gaacggcgcc gtgaaatcgc gaaaaagtac
     1801 acgcgcgcgt tttcagacct tcccggaatt ttgacgccgg tcgaacgctc aaacatgaaa
     1861 cacgtctacc atctttatgt gattaggctt cagggaaagt ttgccggaaa acggcaaata
     1921 ttcttcgagc agttgcataa gcggggaatt ggcgcccagg tccactacat tccgattccg
     1981 gaacagcctt attaccggtc cctgggttat acggcggagg attgtccaaa tgcgagaagt
     2041 tattatgcgt cggctgtttc gttaccgatg tatcctggat tgacgaacac agaggttcat
     2101 tcgattatta aggcagtcaa agatatatgc catcggattt ttagataatg cgacgggaga
     2161 tctcgatgaa aaaaaatgat aaaaatctgc agttgaacgg aaaaaccatt ttggtgacgg
     2221 gtggtacagg gtcctttggg caacgatttg ttcggacaat gcttgaaaac tttaagccta
     2281 aaaagattat cgtgctttct cgtgatgaaa tgaaacagtg gaatatgcag cgggagtttg
     2341 ccgctttgga caaaaaaaat gtcctgcggt tctttatcgg tgatgtgcgg gacaaaggtc
     2401 gacttcatcg ggctttttac ggagtcgatt acgtgatcca tgcggccgca tccaagatta
     2461 tcccgaccgc ggaatataat ccctttgagt gtatcaaaac aaatgttcaa ggcgcgatga
     2521 acgtgatcga ggccgcgatc gatcaaaagg tcgaaaaggt cgtggctctg agtacggata
     2581 aagcgtgtaa tccgatcaat ctctacgggg cgaccaagct gtgctcggac aagctttttg
     2641 tcgcgggaaa ctcttatgta ggaacccgaa atactttgtt ttgtgtcgtt cgttacggaa
     2701 atgtgatggg aagtcgcggg tcagtgatcc cgttttttcg ttcaattgca catacgggcg
     2761 aacttccgat taccgatgag cgtatgacgc gttttatgat cactcttgag gctggcgttg
     2821 agttggtatt gcgcgccttg cgcgagggca ttgggggcga aatatttgtt cccaaaattc
     2881 catccatgaa ggttgtggat gtcgcgggga cgatcgcgcc gaaggcgagg ctcaagattg
     2941 tcggtatccg gccgggcgaa aaaatccatg aaatgatgat tagcgaagaa gatgcccgta
     3001 caacttacga atacggcgct tattacaaaa ttcttcccgc aattcatgat tggcagtatt
     3061 gcggcaagag aatcggttcg gggaagctgt gcccggaagg atttagttat gacagtggta
     3121 ctaacgatga atggatgagc ccctcggatc taagaaaatg gatggatcgg gaatacggaa
     3181 aatcggcgta aaagcggtgg atttatgaaa atagggctca agctgtggtc c
//
LOCUS       DAOSIO010000183         3208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_26240_length_3208_cov_3.175706, whole genome shotgun sequence.
ACCESSION   DAOSIO010000183 DAOSIO010000000
VERSION     DAOSIO010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26240_length_3208_cov_3.175706"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /gene="trpB"
                     /locus_tag="PK590_06755"
     CDS             <1..952
                     /gene="trpB"
                     /locus_tag="PK590_06755"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010997935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOG24332.1"
                     /translation="LKREDLLHTGAHKINNTIAQALLARRLGKTRIVAETGAGQHGVA
                     SATAACVFGLKCVVYMGAVDIERQSLNVYKMKLLGAEVRPVMSGSQTLKDATNEAIRD
                     WVTNVEDTHYLIGSVVGPHPYPMIVRDFQSVIGRETRASFFKNHGGLPDYLLACVGGG
                     SNSMGFFYPFLKDKKVRMIGVEAGGEGVSTKRHASSITAGRPGILHGMKSYLLQDKNG
                     QVQLAHSISAGLDYPSVGPEHSHLSDSGRVQYVSVTDTEALKGFDLLTKLEGIMPALE
                     SAHVVGYLSKLMPKIKKTDRMVICLSGRGDKDIHTVQQYF"
     gene            952..1776
                     /gene="trpA"
                     /locus_tag="PK590_06760"
     CDS             952..1776
                     /gene="trpA"
                     /locus_tag="PK590_06760"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOG24333.1"
                     /translation="MKELDTLMKNRLEEKIKQARQKGRKLFCLFLTFGYPSIAATERL
                     ILSARDSGVDIIELGFPFSDPLADGPTIQYSSQESLKHNPSIRDAMRLVERLRRKGCE
                     LPIVYFGYLNPVFSYGEIKFAGDAKTAGFDGLILPDLPPEEETLLRRECKRKNLCLVN
                     LVAPTTSDERMKMLVRESQGFVYYVSLRGVTGVRRSLPSDLASRVKKIREMTSKPVLV
                     GFGVSTPNQAKLVARISDGVIVGSAVIREIQKGILSAERLIKRISRAVHLEPHKPL"
     gene            1773..2069
                     /locus_tag="PK590_06765"
     CDS             1773..2069
                     /locus_tag="PK590_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOG24334.1"
                     /translation="MKKIKRKACFHVYILQCKNGTYYTGFTNDLKRRFKEHSTGRGAK
                     FLRGKGPFKLVYAKRYAYFLSAMRAERDVKKMSRKQKERLVAEHGNQSGMRLKG"
     gene            2066..2500
                     /gene="ruvX"
                     /locus_tag="PK590_06770"
     CDS             2066..2500
                     /gene="ruvX"
                     /locus_tag="PK590_06770"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOG24335.1"
                     /translation="MRAVLGLDMGGKRIGVAAVQAGTTIALPVGVIERKNREWVLSEI
                     QKLLKSYKAEKIVVGLPVTLKGEMGIAAEKLMAEMEWFKARLAVPVMMWDERLSSKEV
                     ERVLLEGDVSRKRRKEVIDQLAAQRILQSYIDSQSGTGIRVS"
     gene            2516..>3208
                     /locus_tag="PK590_06775"
     CDS             2516..>3208
                     /locus_tag="PK590_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOG24336.1"
                     /translation="MSYNVTKLLNGVRVLTVPMPGRDSAAVMVGVKTEGRYETRKLSG
                     VSHFLEHMLFKGTKNRTTRQIKEDVEGVGGILNAFTGEEVTCYFAKLLKEHYPRALDV
                     LLDMVLNATLPLDEFNREKPVILEEIKMYHDLPSHHAHELMGELLWPGQSLGRSVAGT
                     AESVAKLTRTDMAAYMTRQYQCANILVAVSGTISHAEVVERVQSIYTSRQGKKGLGFV
                     KAESRQTKPRICF"
BASE COUNT          784 a          702 c          964 g          758 t
ORIGIN      
        1 tctgaagcgg gaagatttgc ttcacaccgg tgcccacaag attaacaaca cgatcgcgca
       61 ggcgctcctt gcccggaggc ttgggaaaac ccggattgtg gccgaaaccg gcgctggtca
      121 gcacggagtg gcttcggcga cggcggcttg tgtatttggc ctaaagtgtg tggtttacat
      181 gggggcagtc gatatcgaga ggcagtcgct taatgtctac aagatgaaat tactgggcgc
      241 ggaggtgcgc ccggtgatgt cgggttcgca aactctgaag gatgcgacca acgaggctat
      301 tcgtgattgg gtgaccaacg ttgaagatac ccattacctg attggttccg tggtcggacc
      361 acacccgtat ccgatgatag tgcgggattt tcagagcgtg atcggacggg aaactcgcgc
      421 ctcttttttt aaaaatcacg gtggtctgcc ggattatctt ttagcctgtg tcggaggtgg
      481 tagcaactcc atgggatttt tctatccttt tttgaaggat aaaaaagttc ggatgatcgg
      541 agtggaagcg ggaggggaag gggtttcgac caaacgtcat gcctcttcga ttaccgcggg
      601 gcggcccggt attcttcatg gaatgaaaag ttatctcctg caggacaaaa acggtcaggt
      661 ccaacttgcg cattcgatct cggccggact ggattatcct tcggtggggc cggaacattc
      721 gcacctgagc gattccggca gggttcaata cgtgtccgtg accgatacgg aagcgctcaa
      781 gggttttgat ctgctgacga aactggaagg gattatgccg gcgcttgagt cggctcacgt
      841 cgtcggctac ttgtcgaaat taatgccaaa gataaagaaa acagatcgga tggtgatttg
      901 tttgtcgggg cgcggcgaca aggacattca cacggttcag cagtattttt aatgaaagaa
      961 ctcgacacac ttatgaaaaa ccggttggaa gaaaaaataa aacaggcgcg acaaaagggg
     1021 cggaaactct tttgcctttt tttgacgttt ggatacccga gtattgccgc gacggaacga
     1081 ctaatccttt cagcgcgaga ttccggcgtg gacatcattg agctcggttt tccattttca
     1141 gacccgctcg cggacggccc gaccattcaa tactcttcac aagagtcgct caagcataat
     1201 ccttccattc gcgacgcgat gcggcttgtt gagcgtctgc gtagaaaagg gtgcgaactt
     1261 ccgatagtct atttcggata tttaaatcct gttttttctt atggcgaaat aaagttcgcg
     1321 ggggacgcga aaacggcggg ttttgacgga ctgattcttc ccgatctccc cccggaagaa
     1381 gagacgctac ttcgtcggga atgcaaacgc aaaaacctgt gccttgtgaa tttggtcgct
     1441 ccaacgactt cggacgaacg aatgaaaatg ctggttcggg agtcccaggg gtttgtgtat
     1501 tatgtctcac ttcgcggagt tacgggtgtc cggcgttcct taccgtcaga ccttgcgtcg
     1561 cgtgtcaaaa aaatacgcga aatgacctca aaacccgtcc tggtgggttt tggagtatcg
     1621 acgccgaatc aggccaaatt ggtcgcgcgg atttccgacg gagtgattgt aggcagcgcg
     1681 gtcatccgag agattcaaaa gggaattctt tcggcggagc ggttgattaa aaggatttcc
     1741 cgggcggtcc atttggaacc gcataagccc ttgtgaagaa aatcaaacgg aaagcttgct
     1801 tccacgtcta catccttcag tgtaaaaacg gaacttatta cacgggtttt acgaacgatt
     1861 tgaagcggcg gttcaaagag cattcgaccg ggcggggggc caagtttttg cggggcaagg
     1921 ggccatttaa gctcgtctac gcgaagagat acgcttattt tttgagcgcg atgcgggcgg
     1981 agcgggacgt caaaaagatg tctcgcaaac agaaagagag gcttgtggcg gagcacggga
     2041 accaatcagg gatgaggctg aaaggatgag agctgttttg ggtctggata tgggaggcaa
     2101 gaggatcggg gtcgccgcgg tgcaagcggg aacgacgatc gctttgccgg tcggtgtgat
     2161 cgagcggaaa aatcgtgaat gggttctttc cgagattcaa aaactgctga aatcttataa
     2221 agctgagaaa atcgtggtcg gtctcccggt gacattaaaa ggagaaatgg gaattgcggc
     2281 cgagaagttg atggcggaaa tggaatggtt caaggcaagg cttgcggttc ctgttatgat
     2341 gtgggatgag cgtttatcgt ccaaggaagt ggaacgggtg ctgttggaag gggatgtgag
     2401 ccggaaacgc cgcaaggaag ttatcgatca gcttgccgcc caaaggattt tacaaagtta
     2461 cattgacagc caatcgggga ccgggatccg ggtctcgtag aaaaaaggtt tcccaatgag
     2521 ttataacgtg acaaaacttt tgaatggagt tcgagtcttg acggttccga tgccagggcg
     2581 ggattcggcc gcggtgatgg ttggggtcaa gactgagggc cgctacgaaa ccagaaaact
     2641 ttcgggagtc tctcattttc ttgagcacat gctctttaag gggacgaaaa atcgtaccac
     2701 gcggcagatc aaggaagatg tggagggcgt gggcggtatt ctcaatgcct tcacgggtga
     2761 agaggtaact tgttatttcg cgaagctttt gaaggaacat tatcccagag ccttggacgt
     2821 gcttttggat atggtgctga acgcgacttt gccgctggat gaatttaatc gcgaaaaacc
     2881 ggtcattctt gaagagatca aaatgtacca cgaccttccg tctcaccacg cgcacgagct
     2941 aatgggtgag cttctctggc ctgggcagtc gcttggacgg tccgtcgcgg gaacggccga
     3001 atcggtcgcg aagcttacgc gtacggacat ggcggcttac atgacgcggc agtaccaatg
     3061 cgcgaatatt ctggtggcgg tctcgggaac tatttcccac gctgaggtgg tagaacgggt
     3121 tcagtcgatc tatacttccc gacaggggaa gaaaggccta ggatttgtga aggcagaaag
     3181 tcggcagacg aaaccccgga tctgtttt
//
LOCUS       DAOSIO010000184         3170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_26633_length_3170_cov_4.048475, whole genome shotgun sequence.
ACCESSION   DAOSIO010000184 DAOSIO010000000
VERSION     DAOSIO010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26633_length_3170_cov_4.048475"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(255..830)
                     /locus_tag="PK590_06780"
                     /pseudo
     CDS             complement(255..830)
                     /locus_tag="PK590_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
     gene            complement(907..1278)
                     /locus_tag="PK590_06785"
     CDS             complement(907..1278)
                     /locus_tag="PK590_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOG24337.1"
                     /translation="MSCGSYFYQCLFVAVFFAFAAIFAILPLALAWFLAPKKPFPAKT
                     APYECGLASRGNPWIRYQAQYYLFALIFVLFDVEVIFIYPWAVAFKQLGMFAFLEMVI
                     FIAILSFGLFYAWKKGLLEWE"
     gene            1485..1889
                     /locus_tag="PK590_06790"
     CDS             1485..1889
                     /locus_tag="PK590_06790"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HOG24338.1"
                     /translation="MEIVEVGCAVIIRGGKVLVSQRKVGDSHGGLWEFPGGKRKPDET
                     LENCLAREVREELAVEILPERLLERQDRAYPGGMIRLFFYLCQWISGEPCCLDCQDAR
                     WASAPELAGLQFLPNDQNILARLIHENVIGTY"
     gene            2055..>3170
                     /locus_tag="PK590_06795"
     CDS             2055..>3170
                     /locus_tag="PK590_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG24339.1"
                     /translation="MKTFSAVEKIARIRWPLRISLRYKLAVPVFLLVFAGFSFVMYST
                     FTTVREMMTGYKESRLSTIAEVFAEALRIPMSEGDRKTLQAYVDLVAEQSDVEEVVVE
                     NVEGQCVGDSRRGPHPAPRLFEKQDFSGVRMMDPDRYAAVAPIIAGDQILGRLMIVFT
                     RVEIEKDLRKVFGERFLLASIFALLTTFIVLMITWLGLRPLFTLQRTARLILSGDLGA
                     RARIRSRDEIQEVGDAFNRVVTRLVQSFEHLRTRTRALEESEEKYRSIVNDVSDIIFS
                     ISPEGELILLNRGFSGYTREEILAEGLSAFFKMHVPEDEQKIQDALELIVNTQQPILH
                     MNTRHIHRAHQTDIYYQTNLTPVVDCEGNLRMIQGVMR"
BASE COUNT          773 a          757 c          892 g          748 t
ORIGIN      
        1 caagaagaag cgagtcgcgg aggatccgaa gtttgatccc gggaaacttt ttctcgagag
       61 cgtctttgag tgaattaatc atagagggca taaggttacg ctccggccga aggattgggt
      121 gaagacggag cgctgaccgc cggttcaagg ttcggagctt tcggcttttt gccgattcgg
      181 atcacatgtc cataatgctt tgcccggtta tcggtttccg gaggttgcca gacttccggg
      241 ttaaacttcg gaaccagacc gtgaggccca agttcgggaa tcggaaactc gcgcttaaga
      301 cctttttgat accattcggc tttttggatg ctttgcgaat cgattttttt ctggagttgc
      361 atgaggccgt gtagcagaac ctcgggacgg ggaggacagc cggggatgta tacatccacc
      421 gggataaagc ggtcaatgcc gcgcaagacg ttgtagccat cggcaaaggg gcctcctgag
      481 acggcgcagg cgcccatggc gatcacatat ttcggctccg ccatctgatt aaaaagtcgg
      541 acgacattgg gggccatttt tttggtgacg gttccggcga ctatcatcag gtcggcctga
      601 cgcggtgagg cgcggaaaag ctccgctcca aaacgcgcga tatcataacg cgcggccgcc
      661 gcggcgatca tctcaatcgc gcaacaagcc aggccgaact gcatgggcca gattgatgag
      721 cggcgacccc agttgtagat aaaatcaagg gtcgtgacaa aaatattctg ttttgtcatc
      781 tcatcgcgaa gagccggatc aatcgttgaa ttattttccg tgtatttcat agtcaatgta
      841 ataccgtgag gcgcgtggag tacggaatga aatttccgga aacttcaacc cgcacgccgt
      901 taagggttac tcccattcca gcaatccctt tttccaggcg taaaaaagcc cgaaagaaag
      961 gatcgcgata aaaatgacca tctccagaaa ggcgaacatc cccagttgtt taaatgccac
     1021 ggcccaggga taaataaaga tcacttcgac gtcaaaaagc acaaaaatga gagcgaaaag
     1081 ataatactgg gcctggtacc ggatccaggg gtttccccgg gaggcaagtc cgcactcgta
     1141 gggagcggtt ttcgctggaa acggtttttt gggagccaga aaccacgcca aagcgagcgg
     1201 aaggatcgcg aaaatggccg cgaacgcgaa gaaaaccgcc acaaaaaggc attggtagaa
     1261 ataggaaccg caagacatag aaacgccatt ataaggagaa aacttttcaa gtcaacccgc
     1321 ttacagaaaa gctgattttt atataaaaga gtttcattca aggacttacg actgtaaaat
     1381 gtttctgttt tactctctgg aagtttaaat aaacggcctc caaataaacg ttatttacca
     1441 cagagtttgc ccttcggagt ttgggaaagg gagcaggtta ttacatggaa atagtcgaag
     1501 tcggatgcgc ggtcattatc aggggaggaa aggtcctcgt gtcccagcgg aaagtcgggg
     1561 atagtcatgg aggactttgg gaattcccgg gagggaaacg gaaacctgat gagacccttg
     1621 agaattgcct tgcgcgggag gtacgggaag aacttgcggt tgagatcctc cctgagcggt
     1681 tactggagcg tcaggaccgc gcgtatcctg ggggaatgat ccggcttttt ttctatctct
     1741 gccaatggat aagcggagaa ccgtgctgtc ttgactgtca ggacgcccgc tgggctagcg
     1801 ccccggaact tgccggtctc caatttctcc cgaacgatca gaacatcctc gcccggctta
     1861 tccacgaaaa cgtgatcggg acttactgat gcttttccgg ttgatccaaa aggatcgcta
     1921 aagacgggcg tggagctttc gggccggcct gtgttttgat tttgaagaaa ttgcgcttcg
     1981 ccggaagttt tttatcgata taatgactgt cgactatggc ggagcgcaaa aagcacaaac
     2041 atcacatcgg gcggatgaag acgttttctg cggttgagaa gatcgcccgt attcgttggc
     2101 ctctccggat ctcccttcgc tacaagctcg cggtccctgt ttttctgctg gtttttgcgg
     2161 gattttcctt cgtcatgtat tccacgttca cgaccgtccg cgagatgatg accggttata
     2221 aggaatcccg cctcagcact atcgcggaag tatttgccga ggccctccgg atcccgatga
     2281 gtgagggaga ccggaaaact ctccaggcct atgttgattt agtggccgaa caatctgatg
     2341 tggaagaagt ggtggtggag aatgtcgaag gccagtgcgt cggagacagc cggaggggac
     2401 ctcatcccgc tccccgtctt tttgaaaaac aggatttttc aggggtgcgg atgatggacc
     2461 cggacagata tgccgccgtg gccccgatta tcgcgggcga ccaaattctt ggccgcctga
     2521 tgattgtttt taccagggtg gaaatcgaaa aggatctccg gaaagttttt ggcgaaaggt
     2581 ttttgctggc ttccatcttt gccctcctca cgacttttat tgtgctgatg attacgtggc
     2641 ttggtcttcg gcctcttttt acgctccagc ggacggcccg tttaatcctt tcaggggatc
     2701 ttggagcccg ggccaggatt cgatcccggg atgagattca ggaggtgggg gacgccttta
     2761 acagggttgt taccaggctc gttcagagtt ttgagcattt gcggacgcgg actcgcgcgc
     2821 tcgaggaatc ggaagaaaag taccgctcca tcgttaacga cgtgagcgat atcatttttt
     2881 ccatttcacc cgaaggggag ttaatccttc tgaaccgggg attttccggg tacacgcgcg
     2941 aggaaatcct ggcggaagga ttgtcggctt tcttcaagat gcatgtgccg gaagacgaac
     3001 aaaaaatcca ggacgcgctt gagttgatcg tgaacaccca gcaaccgatc cttcacatga
     3061 acacccggca cattcaccgc gcgcaccaga cggacattta ctaccagacg aatttgacgc
     3121 cggtggtgga ctgtgagggc aatttgcgca tgatccaggg tgtgatgcgt
//
LOCUS       DAOSIO010000185         3161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_26733_length_3161_cov_2.620090, whole genome shotgun sequence.
ACCESSION   DAOSIO010000185 DAOSIO010000000
VERSION     DAOSIO010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26733_length_3161_cov_2.620090"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PK590_06800"
     CDS             <1..819
                     /locus_tag="PK590_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOG24340.1"
                     /translation="AIQGGVLTGDVKDILLLDVTPLSLGIETLGGVMTKLIERNTTIP
                     TKKSEIFSTAADNQTAVTVKVLQGEREMANDNRLLGLFNLEGIPPAPRGVPQVEVTFD
                     IDANGIVNVSAKDLGSGKEQKIRIESSSGLAKEEVEKLVKEAQSHAEEDKKKHEVISA
                     RNQLDNLIYATEKALKEHGDKVSEDEKKKVEEALKDAKTAVTADSKETIEQASQKLAT
                     ASHTIAQKMYEEAAKKASAGKGAPQADPEKKEDGVVDADYEVVDENEKDKKKDK"
     gene            854..1726
                     /locus_tag="PK590_06805"
     CDS             854..1726
                     /locus_tag="PK590_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24341.1"
                     /translation="MTRKVTVLGAFFALAMSCCYGVEAAPSFRLQSPPKESTAQTPFH
                     YRLILEWPSGEGSYEVAPVSPVLENLELIEQRQSQETAGSRLTVTLAFTFSGIKKGPA
                     RIGPLQIPYRREGETDWQMIPVPAQTISIRARFPWSMFLRVGGLILTVSLCLYLGRKA
                     FVVSRAAKLEKNLQPYDPKQRVYAKSEEHILTFKGVTQQETLSHWIRAVKETVMTYYG
                     LPNKPTTESEIIQALASQKLRVEEMNEITRIFRETESLKFNGQLLTTREMETLQKTLL
                     KYVRGKIIIEKSMV"
     gene            1811..2800
                     /locus_tag="PK590_06810"
     CDS             1811..2800
                     /locus_tag="PK590_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOG24342.1"
                     /translation="MDASIQELNSKIRETSSIIKDLTREIGKVIVGQQYLIDRLLMAL
                     LADGHILIEGVPGLAKTLSVRTLSQAIAAQFQRIQFTPDLLPADVIGTLVYNPKDGNF
                     TTKKGPIFTNIVLADEINRAPSKVQSALLEAMQERQVTIGNETFPLPVPFLVLATQNP
                     IEQEGTYPLPEAQVDRFMLKLVVTYPTQKEEKEILQRMASGKSIQIQPVIDLKKIMDL
                     RQLMHQIYVDEKVKDYILDLVFASRNPEQYNLADLKPLISYGGSPRASIMLTLAAKAN
                     AFLQGRGYVTPDDVKQIGMDVLRHRVLLTYEAEAEDLTSEDIIRNIFDQVEVP"
     gene            2872..>3161
                     /locus_tag="PK590_06815"
     CDS             2872..>3161
                     /locus_tag="PK590_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOG24343.1"
                     /translation="MIPVELLRKIRKIEIHTTRLVNDLFGGEYESVFKGQGIEFADVR
                     EYVPGDDIRTIDWNVTARSNQAFVKKFVETRELTVIFAVDMSGSQYYGSQDKL"
BASE COUNT          862 a          778 c          802 g          719 t
ORIGIN      
        1 gcaatccagg gcggggtcct gacaggggac gtcaaggaca tccttctttt ggatgtcacg
       61 ccgctttccc tgggcattga aaccctgggc ggggtgatga ccaagctgat tgagcgtaac
      121 acgaccattc caaccaaaaa atctgagatt ttttcgaccg cggcggataa ccaaaccgct
      181 gtcacggtga aggtcctcca gggggaacga gagatggcta acgataaccg tctcctgggc
      241 ctttttaacc tcgagggcat tcctccggcg ccccggggag ttccccaggt ggaagttacc
      301 tttgacatcg acgcgaacgg gattgtgaac gtttccgcga aagatctggg ctccggaaaa
      361 gagcaaaaaa tccgcattga atcttcttcc ggtctcgcca aagaagaagt cgaaaaactg
      421 gtcaaggaag cccagtcgca cgcggaagaa gataaaaaga aacatgaggt catttcagct
      481 cgtaatcagc tggataacct gatctatgcg accgaaaagg ctctgaagga gcatggagat
      541 aaggtttccg aagatgaaaa gaaaaaggtc gaagaagctc tcaaggacgc caagacggcg
      601 gtgacggctg attccaagga aacgattgag caggcatcgc aaaaactcgc gaccgcgtct
      661 cataccatcg cgcaaaaaat gtatgaagaa gccgcgaaaa aagcaagtgc cgggaaggga
      721 gcgccgcagg ctgacccgga gaaaaaagag gatggcgttg tcgatgcgga ttatgaggtt
      781 gttgacgaaa acgaaaaaga caaaaagaaa gacaagtaat cgctcctttt catcatttaa
      841 gaaggatgcg gccttgacgc gcaaggtgac ggtgctaggt gcgtttttcg cccttgcgat
      901 gagctgttgc tatggcgtcg aggccgctcc ttcattccgt cttcaatccc ccccaaaaga
      961 atctaccgcc caaactccct ttcactaccg tcttatcctt gaatggccat cgggggaggg
     1021 gagttatgaa gttgcccctg tcagtcccgt gctggaaaac cttgagctta tcgaacaacg
     1081 ccagtcgcaa gaaacagcgg gatcccgttt gacggtaacg ttggctttta cgttttccgg
     1141 tattaagaaa ggccccgccc gtatcgggcc tctccaaatt ccatatcgtc gggaagggga
     1201 aactgattgg caaatgattc ccgttcccgc tcagacgatc tcgatccgcg cgcgatttcc
     1261 ctggtccatg tttttgcgtg tgggcgggct gatcctcacg gtttctcttt gtctttattt
     1321 gggccgaaaa gcttttgtag tttcgcgagc ggcgaaactg gaaaaaaacc tccagccgta
     1381 tgatcccaaa cagagggtct acgcgaaatc ggaagagcat attttaactt ttaagggagt
     1441 aacacaacag gaaacgctct cccactggat tcgtgccgtc aaggagacgg tcatgaccta
     1501 ttacggcctt cccaacaagc cgacaaccga atcggaaatc attcaagctc tcgcttccca
     1561 aaagctgcga gttgaggaaa tgaacgagat tacccgtatt tttcgtgaaa cagaatccct
     1621 gaaatttaac ggacagctcc tcacgacccg tgagatggaa accctgcaaa agacactctt
     1681 gaaatatgtg cggggcaaga ttatcattga aaaatcaatg gtctgaaaag gatgctcggg
     1741 gcaatgtttg tccctctcat ctgctcaatt ttatcctgca ctttaaattt taaaaataag
     1801 gagaagaaca atggacgcgt ccatccagga acttaatagt aaaattcgag aaacaagctc
     1861 gatcatcaag gacctcaccc gggaaatcgg caaagtcatc gtcggccaac agtatctgat
     1921 tgaccggctt ttaatggcgc ttttggccga cggccacatt ctgatcgagg gggttcccgg
     1981 tctcgcgaag accttgtcag tccgaaccct ttctcaggcg atcgcggcgc agttccagag
     2041 aattcagttc acacccgact tgcttccggc ggacgttatc ggaacactgg tctataatcc
     2101 caaggatggc aattttacga ccaaaaaagg gcctatcttc accaatatcg tcctggcgga
     2161 tgaaattaac cgcgcgccct ccaaggttca aagcgctctc cttgaagcta tgcaggaacg
     2221 ccaggttacc atcggcaatg aaacgtttcc tcttcccgtt ccgtttctgg ttctcgcgac
     2281 tcaaaacccg atcgaacagg aggggaccta cccgttgccg gaagcgcagg ttgaccgttt
     2341 tatgcttaaa ctcgtggtga cttatccgac gcaaaaagaa gagaaggaaa tcctgcaacg
     2401 gatggcaagt ggaaaatcga tccagatcca gccagtgatt gacctgaaaa agattatgga
     2461 tctgaggcag cttatgcacc agatttatgt cgatgaaaaa gtcaaagact acatcctcga
     2521 ccttgttttt gcgtctcgta atccggagca atacaatctc gcggatctga aaccgctcat
     2581 ctcttacggt ggatcgcctc gtgccagcat catgcttacc ctggcggcca aggcgaacgc
     2641 cttcctccag ggacgcggtt atgttacgcc ggacgacgtg aaacaaatcg gaatggacgt
     2701 tcttcgccat cgcgtattgc tgacttatga agcggaagcg gaagacttga cctccgagga
     2761 tatcattaga aatatttttg accaggtgga agtgccataa ccaggattaa aacacgaaat
     2821 tcttggtttg aaatccgata agcgcattct caaacagaaa ggttttgaga agtgataccc
     2881 gttgagcttt taagaaagat tcggaaaatt gaaattcaca ccacccgtct ggtcaatgac
     2941 ctttttgggg gagaatacga atctgttttt aaggggcagg gtattgaatt cgcggacgtc
     3001 cgggaatatg ttcccgggga tgacatccgg accattgact ggaacgtcac ggcgcgctct
     3061 aaccaggctt ttgtgaaaaa gtttgtggaa acccgggagc tgacggttat cttcgcggtc
     3121 gacatgtccg gttctcaata ctacggttct caagacaaac t
//
LOCUS       DAOSIO010000186         3150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_26844_length_3150_cov_4.682068, whole genome shotgun sequence.
ACCESSION   DAOSIO010000186 DAOSIO010000000
VERSION     DAOSIO010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26844_length_3150_cov_4.682068"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..2514
                     /locus_tag="PK590_06820"
     CDS             202..2514
                     /locus_tag="PK590_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOG24344.1"
                     /translation="MPQVQAPINTGADLREALPSRFKTARSTILFDRFMTHFIKIGGI
                     GIIVAVAAIFFFIFAQTLPLMSRAKVTLLETLSVPAKEYKALGIDEWAEKPFLVEKSG
                     RVTFYDLVSKKIVYEFTPDDFLGSKVLAVKYDPLKQRFIYGTGDGNISIFQLHYDASY
                     EGDQRLIMPRIEPLITAPVLKSEDPVRVRDLASGDSGETMLVAGIFATGEKTSVPVAI
                     VTRESSLMGFSEPQLEKVIDLSSDLPGKPIRILVNSSADGVLAATEEGDVHYFRVTPD
                     VVQLRQSFRPFEDQEDQRIGSLDYINGEVSVSVTNGKGLNRIFSLAIPQGEKRRIFVK
                     TKEFEPIGGAADFFSASLRNKAFLTGHGSRVSLRYSTTETVRWEKRFPFEVKLGALSP
                     QYDRILLLDSKHQLHFYNLKDPHPQAGWKAFFKKLWYEGYPEADFVWQTSGSTDDFEP
                     KLSMVPLIIGTLKGTWYALIFSLPIALLGALYTSQFAHPKMKLFIKPTLEIMASLPSV
                     VLGFLAALWLAPLIETKVPSLMVVCVLIPVGAMMFGAFWGHLPIRYRKWIPLGFEWLA
                     IIPVLALLGYWGWQLGPVIEKTFFVFHDPDLGIRTADFRLWWPQATGTPFNQRNSLVV
                     GFMMGFAVIPIIFTIAEDALSNVPKSLISGSLALGASRWQTAVRVVLPTAFPGIFSAI
                     MIGLGRAIGETMIVVMATGNTPVMNFSIFSGMRTLSANIAVELPEAPYLGTLYRTLFL
                     GAVILFVMTFIVNTVAEVVRQRFRAKYKTV"
     gene            2514..>3150
                     /locus_tag="PK590_06825"
     CDS             2514..>3150
                     /locus_tag="PK590_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24345.1"
                     /translation="MMTLATAKPLFDKKGRSRGESFVWLTSTGLAIGLAMIVYILSVI
                     FTNGIKIFWPPAVAIFELEGEPSDGFSGRIAGVIAKRQFKMVHDPEDHKIEEWQIFLG
                     NREVYGTSYKFFDKSSIREIRYPRDVLRLERREYGDALVFPVSLKIAGKEKISFGDPG
                     FEEVLSRLVRETNERWKEIHQIEKKEIGLINHKIEKLRLREKSLRERGLFAG"
BASE COUNT          701 a          738 c          883 g          828 t
ORIGIN      
        1 tccaagcagg gacaggagct tgtggtcaag gatggttatt atccgctgac cgccaagctg
       61 attgaagagg aacttagcaa gctacagtaa gagaaagcgg ttttctgaaa ttgcaaaaaa
      121 cgaaccagcc ggcccgttgt caagttcagc ggaccggctg gtttgtaaaa tatcctttaa
      181 agccaaggga ggataaaatc gttgccacag gttcaggcgc ccatcaatac cggagctgac
      241 ttacgggagg ctctcccttc gcgttttaaa acggcccgct cgaccatcct ttttgaccgg
      301 ttcatgacgc atttcataaa gatcggcggg atcggaatta tcgtggccgt ggccgcgatc
      361 tttttcttca tttttgccca gacccttccg ctgatgagcc gggcgaaagt gacgcttctt
      421 gagacgcttt cggtccccgc gaaagagtac aaggcgcttg gcatagacga atgggccgaa
      481 aaaccttttc tagttgaaaa atccgggcgg gtcacttttt atgacctggt ctcaaaaaag
      541 atcgtttatg aattcactcc ggatgacttt ctcggttcta aagttctcgc ggttaaatat
      601 gatcccctca aacagcgttt tatctacgga accggagacg gcaatatcag tatctttcaa
      661 ctgcattatg acgcttctta cgagggagac cagcggttga tcatgccgcg gatcgagccg
      721 ctgatcactg ccccggtttt aaagtcggaa gacccggttc gtgtgcgtga tctggcgagc
      781 ggagattccg gagaaaccat gcttgttgcc gggatcttcg ctaccggtga aaaaacatcc
      841 gttcccgtgg cgattgttac gcgtgaatca agtctgatgg gtttcagcga acctcaactt
      901 gaaaaagtga ttgatctttc gtccgacctg ccgggaaaac ccatccggat tcttgtcaat
      961 tcaagcgcgg acggagtcct ggccgcgacg gaagaagggg atgtgcatta ttttcgggtg
     1021 acaccggacg tggttcagtt gcggcaatct ttccgtccct tcgaagacca ggaagatcag
     1081 cggataggct ctctggatta catcaatggg gaagtgtccg tttcggtcac taacgggaaa
     1141 ggactgaaca ggattttttc gctggcgatc ccgcagggag aaaagagacg gatttttgtg
     1201 aagacaaagg aatttgagcc gattggaggc gccgctgatt tttttagcgc gagtctccgg
     1261 aacaaggcgt ttctgacggg acatggctcc cgggtgtctt tgcgttattc gacgacggag
     1321 acggtgcgct gggagaaacg ttttccgttc gaagtgaagc tcggggccct gagcccccag
     1381 tatgaccgca tccttttgct cgattcaaaa catcaattgc atttctacaa cctgaaggat
     1441 ccgcatcccc aggcgggatg gaaggctttc ttcaagaaac tctggtatga gggctaccct
     1501 gaagctgatt ttgtctggca gacgagcggt tctaccgatg actttgagcc gaaactttcg
     1561 atggttccgc ttattatcgg aactctgaaa gggacctggt acgcgctgat tttttctctt
     1621 cccatagcgc ttttgggcgc gctttatacg tcccagttcg cgcatccgaa gatgaaactt
     1681 ttcatcaagc ccaccctgga gattatggcc tcgcttccgt ccgtggtgct tggatttctg
     1741 gcggcgcttt ggcttgcgcc gctgatcgag accaaagtgc cgtccctgat ggtggtatgt
     1801 gttttaatcc cggtcggtgc gatgatgttc ggggccttct ggggtcattt gccgatccgc
     1861 tatcgtaaat ggattccgct tggttttgaa tggctcgcga tcattcccgt gcttgccctt
     1921 ttgggttact ggggatggca gttgggtccc gtcatagaaa aaacgttttt tgttttccat
     1981 gacccggatc tcgggatacg gacggcggat tttcggctct ggtggcccca ggcgacaggg
     2041 acacccttta accagagaaa ctcgcttgtc gttggcttta tgatgggatt tgccgtgata
     2101 ccgattattt tcaccattgc cgaggatgcg ctttccaatg tgcccaagag ccttatttct
     2161 ggatccctgg cgcttggagc aagccgctgg caaacggcgg ttcgcgtggt gctcccgacg
     2221 gcgttcccgg ggatcttttc cgcgatcatg atcggccttg gccgggcaat cggagaaacc
     2281 atgattgtgg tgatggcgac cgggaacacg ccggtcatga attttagtat tttttcggga
     2341 atgcggactc tttcggcgaa tatcgcggtc gaacttccgg aagcgcctta tttgggaact
     2401 ctttaccgaa ccctgtttct gggggctgtg attctgtttg tcatgacctt tatcgttaat
     2461 acggtggccg aagtggtgcg acaacgtttt cgggccaaat acaagacggt gtaatgatga
     2521 ctctggcgac ggcaaaacct ctatttgata aaaaaggaag aagccgcggg gagtcttttg
     2581 tttggcttac ctcgacgggg ctggcgattg gtctggcgat gattgtctac atcctctccg
     2641 ttattttcac gaatggcatc aagattttct ggccgcccgc tgtcgccata tttgaacttg
     2701 aaggggaacc ttctgacgga ttttccggaa gaatcgcggg cgttatcgcc aaaagacaat
     2761 tcaagatggt tcacgatccc gaagaccaca agatcgagga atggcagatt tttctcggta
     2821 accgggaggt ttacggaacc agctacaagt tttttgacaa aagttcgatc cgggaaatcc
     2881 ggtacccgcg ggatgtttta cggcttgagc ggagggagta tggggacgcc ctggtgtttc
     2941 ctgtttctct gaaaattgcg gggaaagaga aaatttcttt tggcgatcct ggtttcgagg
     3001 aggttttgag tcgccttgtc cgggagacaa atgagcgctg gaaagagatt catcagattg
     3061 aaaaaaaaga aattggtttg atcaatcaca agattgagaa actgagactt cgcgaaaagt
     3121 ccttgcggga acgagggctg ttcgcgggcg
//
LOCUS       DAOSIO010000187         3060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_27827_length_3060_cov_3.775042, whole genome shotgun sequence.
ACCESSION   DAOSIO010000187 DAOSIO010000000
VERSION     DAOSIO010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27827_length_3060_cov_3.775042"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..>3060
                     /locus_tag="PK590_06830"
     CDS             105..>3060
                     /locus_tag="PK590_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24346.1"
                     /translation="MMATQQSAFKPWLRSIALSTCVIFTFTSVVFDTGFSEVHAAASQ
                     AQIPATTGPHLLSIDSLVDRPDLPDTYGTVKTTFKGKRGAMVVHIQDAHINEEAQRNI
                     GNIIRHFNEKYQLGLVNLEGASGELYTELFSFFPNRDARKNVADYFLREGRLTGPEHL
                     AIVDRTPVLLNGVEDPELYEENRQAYVEALDFKARDEEVLAKLGKLMDGVSRFVFPGE
                     IRELNRRRQNFQEGGREMVAYVRYLVELAHRNQISLNDYPSMHSLLKLVDLEKQVDFN
                     QAEKEVDLLVNDLKKMISREKLTRFLTNTVHFRMKKMKRSEYYGYLQDEVLAAFDMAS
                     DPEALRAQYANVLSYISYMRLYDTVDVSIFEEIDQLERVVKQKLFTNQDQVRLDHMFR
                     IFDIYRKMFEFTLTKQDAEFYYTYQDEFKASTFSDFLKPLIEKHHFSYGLPSQLEILD
                     RDLPRVERFYEAALKRDQVLIERAVEKTTERGAKISAIVTGGFHTPGIERYLRDHDIS
                     YMVVTPRISKAIDQKRESALYDAALRETPLSIENVLNETFLQPKSGVLNDPRFQLAAE
                     HLITALGAELNPAAQAMIRMMAYVSVLDGRAPEETAAIEKAMETLSDQAKGKSLVTAV
                     VGELKQAVAYKNAILLPVAGDAEKVRIIAIVGSERGSEVKIAGMKDRKSIPIFEGKIF
                     LTGVIDVDLAPAEMRGAINSLLKRSEVRFGDVLVQSGTLPPGVSTGLGVKKTTAQEKA
                     IKVAQEQQVRYEKNLKLYQDAGFEVPTKENAQAAFRAILLNANFEDKMAALAETGASK
                     AFKDGIIDRVMIVRLLGRHYSADTIRARVPRLLASDVPVNTYTMTFRSDADIDAYRQR
                     MVDRQTPRSKTGAAQPGAGVVSETEPVGMKPVKLEEPQLVEKMVSSLARVLFIGRLKK
                     DPVSDNSDDQKALEAISEEVRTTLGADRLSRIASAKRLVGEQGLYEILLSMVEELRQD
                     YESPRGLSVDAYP"
BASE COUNT          747 a          760 c          825 g          728 t
ORIGIN      
        1 actaggttta taaagaattt aatctagttt atatctggtt atattttctc ttggatgaac
       61 ttaaacttga atacatttaa aagaggaagg acggcaatcc gaccatgatg gcgacacagc
      121 aatctgcttt taaaccttgg ctcagatcta tcgctttatc cacttgcgtc atcttcactt
      181 tcacttcagt tgtttttgat accggattta gcgaagttca cgcggccgct tcccaggccc
      241 aaatacctgc gacgacgggc ccccatcttc tttccataga ttcccttgtg gatcgcccgg
      301 accttcctga cacctatgga acggtcaaga cgactttcaa ggggaaacgg ggcgcgatgg
      361 tcgttcatat ccaggacgct cacattaatg aagaagccca gcgaaatatc ggcaacatta
      421 ttcgccactt caacgaaaag tatcagctcg ggcttgtgaa ccttgagggc gcgagcggag
      481 aactttacac ggaacttttt tcgtttttcc cgaatcgcga tgcccgaaaa aacgtggcgg
      541 actattttct ccgcgaaggc cgcttgacgg ggccggagca tctcgcgatt gtcgatcgca
      601 cgccggtgct tttgaacggg gtggaggatc ccgaactcta cgaagaaaac cgccaggcct
      661 atgtcgaggc gcttgatttc aaggcccgag acgaagaagt cctcgcgaaa cttggcaaac
      721 tcatggacgg ggtgagccgc tttgtgttcc ccggggaaat ccgcgaactg aaccgccgtc
      781 gtcagaattt ccaggaaggc ggccgcgaaa tggtcgctta cgtccgttac ctggttgagc
      841 tcgcgcatcg aaaccagatc tcgcttaacg attatccttc catgcactcc ttgctcaagc
      901 tggttgacct cgaaaaacag gtggatttca atcaagctga aaaagaggtc gacctcctgg
      961 tcaacgatct caagaaaatg atttctcggg aaaaactgac ccgtttcctg accaataccg
     1021 ttcatttccg aatgaagaaa atgaaacggt cggagtatta cggctacctt caagacgagg
     1081 tgctggcggc ttttgatatg gcttcagacc ccgaggcgct acgggcgcag tacgcgaacg
     1141 tgctttccta tatcagttac atgcgcctct atgacaccgt tgacgtcagt atttttgaag
     1201 aaattgacca gcttgaacgg gtcgtcaagc aaaagctttt cacgaatcag gatcaggtca
     1261 ggctcgatca catgttccgg atttttgata tctaccggaa aatgtttgag tttacgctga
     1321 cgaagcagga tgccgagttt tattacacgt accaggatga attcaaggcg tcgacttttt
     1381 cggattttct caagccgctc atcgaaaagc accacttctc ctacggattg ccgtcccaat
     1441 tggagatcct cgatcgggat ctgccgcgcg tggagcgctt ctatgaagcc gctctcaaac
     1501 gcgaccaggt ccttatcgag agagcggttg agaaaaccac cgagcgcggg gcgaagatct
     1561 ccgcgatcgt gaccggcggg ttccacaccc cgggaattga acgatacctt cgtgaccacg
     1621 acatctctta tatggtagta accccgcgta tttcaaaagc gattgaccag aaaagggaat
     1681 ccgctcttta tgacgccgcg ctccgcgaga cgccgctttc gatagaaaat gttctgaacg
     1741 agacgttcct ccagccgaag tcgggtgtcc tgaacgaccc gcgatttcag ttggcggcag
     1801 agcatctgat tacagcgctg ggggcagaac tgaatccggc ggcccaggcg atgattcgca
     1861 tgatggccta tgtcagcgta ctggatggcc gggcgcctga agaaacggcc gccatcgaaa
     1921 aagcaatgga aacgctttct gaccaggcaa aaggtaagtc tcttgtgacc gctgtggtgg
     1981 gggaattgaa acaggcggtc gcttacaaga atgcgatcct gcttcctgtt gccggagacg
     2041 cggaaaaagt tcgcatcatt gccatcgtgg gttccgaaag aggttccgaa gttaagattg
     2101 cgggtatgaa agacaggaag tccattccga ttttcgaagg aaaaattttc ctcacaggag
     2161 ttatcgatgt tgacttggcc cccgcggaga tgcgtggtgc gatcaactcg ctactgaaac
     2221 gctctgaagt tcgctttggg gatgtcctcg tccagtctgg cactcttcct ccaggtgttt
     2281 ccaccgggtt gggcgtcaag aagacgacgg cgcaggagaa agccatcaaa gtggcccagg
     2341 aacagcaggt tcgttatgag aaaaacttga agctttatca agatgccggc ttcgaggttc
     2401 caaccaaaga aaatgcccag gcggcatttc gggctattct cttaaatgcc aacttcgaag
     2461 ataaaatggc agcgttggca gaaaccggtg cgagcaaagc gtttaaggac ggcattattg
     2521 atcgcgtcat gattgtccgg cttttggggc ggcattatag tgcggatacg atccgcgcgc
     2581 gtgttccgcg ccttctcgct tcagatgttc ctgtgaacac ctacactatg acttttcgaa
     2641 gcgatgcgga tattgacgcc tatcgtcagc ggatggttga tcggcagacg ccacggtcca
     2701 agacgggtgc ggcacaacca ggcgctggag ttgtttcgga gactgagccc gtggggatga
     2761 agccggtaaa gctggaagaa ccgcaacttg tggaaaaaat ggtttcatca ttggctcggg
     2821 tgctttttat aggtcggctg aaaaaagatc cggtttcaga caattccgat gaccagaagg
     2881 ctttggaagc catttccgag gaggtcagaa ccactctggg agcggatcgc ctgtcacgga
     2941 ttgcttcggc gaaacggctt gtcggtgagc aagggcttta tgaaattctt ttgtcgatgg
     3001 tggaggaatt gcggcaggat tacgaatctc cgcgaggtct ctcggttgac gcgtaccctc
//
LOCUS       DAOSIO010000188         3040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_28108_length_3040_cov_2.765159, whole genome shotgun sequence.
ACCESSION   DAOSIO010000188 DAOSIO010000000
VERSION     DAOSIO010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28108_length_3040_cov_2.765159"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PK590_06835"
     CDS             <1..640
                     /locus_tag="PK590_06835"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOG24347.1"
                     /translation="PMIRALIFDFDGVILESADLKTKAFEQIAREEFPGFVEEIVAYH
                     RRNMGISRFVKFRYIFDTLLKKKMTPKIEKRLGDRFTEIIFESLKTVPFVPGSLEFLE
                     AAFGKYRMFVASGTPERELRKVADLRGVTKYFDELHGSPRTKKDIVLDIMARYRFTPG
                     EVLFVGDAESDYLAAKLTGVEFVGRVHDDQLAGCECRQIPDLYHLEKFLPMR"
     gene            644..1546
                     /locus_tag="PK590_06840"
     CDS             644..1546
                     /locus_tag="PK590_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG24348.1"
                     /translation="MSKEKKSTKRETVIVFGGSGFLGSHVCDALADRGYKVRIFDAKP
                     SSYLRAGQTMVVGDILDRKMVFRAVEGCDYVYNFAGIADLDDASTKPLDTVHLNIAGS
                     INIMDATLKAKAKRYIYASTIYVYSEKGGFYRCSKQAAELYVEEYQRRFGLDFTILRY
                     GTLYGPRADARNSIHRYLIEALRHGAITCAGSTDSVRDYVHVSDAATLSVQILASQYS
                     RKHIIISGLHPIKFKEMILIIEEILGKKLSVVTPNRESAAHYDYTPYSFVPKIGSKLT
                     SNLYTDLGQGILECLHEIHAKNQK"
     gene            1561..2340
                     /locus_tag="PK590_06845"
     CDS             1561..2340
                     /locus_tag="PK590_06845"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOG24349.1"
                     /translation="MRLLQSVSNRFKSLYLEVVNKIQVFHELDYPKRRLFLCNNIRLA
                     GCHKEPDTVRWIEGFEKDDVVFDVGANVGAYSLIMACYAKHVYSFEPAFMNFSLLCKN
                     IHENVRLGTISNNLTPFNVALAEKTGLETLNYVNVNLGKSGHQIGGRTIDGYGKSFSP
                     TYFQKVMAFSLDSLIETFAIPTPNHMKIDVDGIEFEILMGGDKTLSRPELRSLLVEAD
                     FNLEKYKNLVKYLVDKGFKLNDKLRITGVESNYGNCLFTRA"
     gene            2533..>3040
                     /locus_tag="PK590_06850"
     CDS             2533..>3040
                     /locus_tag="PK590_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24350.1"
                     /translation="MDEFLIVDEWNEINAWSCGVAAEFTKEARDGGFFREADVPSCLY
                     LFFAYILVSALKNFCFASKIMATPGLKQCVVFQGASRAAFPAFSGNQFLNYFLRYFCS
                     ERNIELRDVVIAPVDAPKTFPPHARPLRMVNFVKKILCALTQGKNIVPRGAICVYGSL
                     HHLKSVVLE"
BASE COUNT          772 a          638 c          752 g          878 t
ORIGIN      
        1 tccaatgatc cgcgcgctga tttttgattt tgacggggtg atccttgaat cagcggatct
       61 caaaacaaaa gcctttgaac agattgctcg ggaggaattt cccgggtttg tggaagagat
      121 tgttgcttat catcgaagaa acatggggat ctcccgtttt gtgaagttcc ggtatatttt
      181 tgatacgtta ttgaaaaaaa aaatgacccc gaaaatcgaa aaacggctgg gggaccgctt
      241 tacagagatc atttttgaga gtctgaaaac cgtccctttc gtgccgggca gtctggagtt
      301 ccttgaagcc gcctttggta aatacaggat gttcgttgcc tcgggaaccc ccgagcggga
      361 actccggaaa gtcgctgacc ttcgaggcgt gacgaaatat tttgacgaac tacacggttc
      421 gcctcgaacc aagaaggata ttgttctcga tatcatggcc cgctaccgtt tcacgccggg
      481 tgaggttttg ttcgtggggg acgcggagtc cgattatctt gcggctaaat taacgggagt
      541 agagtttgtc ggacgggtcc acgacgatca gttggccgga tgtgaatgtc ggcagatccc
      601 ggatctctac catctggaaa aattcttgcc aatgaggtga tttatgtcca aagaaaaaaa
      661 atcaaccaaa agagaaactg tgattgtgtt cggcggctcc ggcttcttgg ggagccatgt
      721 ttgtgatgct cttgctgacc gtggatacaa agtcagaatc tttgatgcca aaccctcctc
      781 ttatttgcga gccggacaga ccatggttgt aggggatatt ctcgaccgta aaatggtttt
      841 cagagccgtt gaaggctgtg actatgttta taattttgcc ggaatcgctg atcttgatga
      901 cgcgagtaca aaaccgcttg atacggttca tctgaatatt gcgggaagca tcaacattat
      961 ggacgccacg ctaaaagcga aggcaaaacg ctatatttat gcgagcacga tctatgtcta
     1021 ttcagaaaaa ggggggtttt atcgctgtag caaacaggcc gccgaactat atgtcgagga
     1081 gtatcagcga cgtttcggat tggacttcac aattcttcgg tacggcactc tttatggtcc
     1141 ccgtgccgat gcgcgtaata gtatccatcg ttatctgatc gaggctctgc gtcatggcgc
     1201 aattacctgt gccggtagca ccgacagtgt tcgagattat gtccacgtta gcgatgcggc
     1261 taccctgagc gtgcagattc ttgcgtccca atacagccgc aaacacatca tcatcagcgg
     1321 tcttcacccg attaaattca aggaaatgat acttatcatt gaggagattc ttgggaaaaa
     1381 attatcggtt gtcacgccga acagggagag cgccgcccat tatgattaca cgccgtattc
     1441 ttttgtgccg aaaattggct cgaagttaac gagtaatttg tatacggatt taggacaggg
     1501 catcttggag tgcttgcatg aaatccacgc taaaaaccag aaatgaaagg aaggcctccg
     1561 atgagacttt tgcaatcggt aagcaatcgt tttaaatctc tctatcttga agttgtaaac
     1621 aaaatacagg tctttcatga gcttgattat ccaaagcgcc gcttatttct ctgtaataat
     1681 atccgcttgg cgggatgtca caaagagccg gatacggttc gttggattga aggatttgag
     1741 aaagatgatg tggtttttga tgtcggggcg aacgtcggtg cttattccct tatcatggca
     1801 tgctacgcta aacacgttta ctcttttgag cctgcgttca tgaatttctc gctgttgtgc
     1861 aaaaatatcc acgagaatgt gaggctcggt acgatttcga acaaccttac acccttcaat
     1921 gttgcgctcg cggagaagac aggcttggaa acgcttaatt atgtaaatgt gaatcttggg
     1981 aaatcgggtc accagattgg cggaagaaca atcgatggct atggcaagtc cttttcgcca
     2041 acttatttcc aaaaggtaat ggcctttagt ttagactcct tgatagaaac ttttgctata
     2101 cccacaccta atcatatgaa gattgatgtg gacggcattg aatttgaaat cctcatgggt
     2161 ggagataaaa cattgtctag accggagcta cgttcgcttc ttgtagaagc ggattttaat
     2221 ttggaaaaat ataaaaattt ggtcaaatat cttgttgata aaggttttaa gcttaacgac
     2281 aagcttcgga taacgggagt cgagtcaaat tatggtaatt gcctttttac acgcgcataa
     2341 cagaattaac tttatgcaat ttagtcatac cttaactgct ctgattgctc catgaactgc
     2401 ggtgcgctgt cgaacaatac tcagctttcg ggcttttttc gtgctggtta tcggcttagc
     2461 gaatatcaat ggttctgtga taacccttgt ttttttgatc atttgaccag tgcgggaatc
     2521 ccctataaac ctttggacga atttttaatc gtcgacgaat ggaacgaaat taatgcgtgg
     2581 agttgtggtg ttgcggcgga attcacgaag gaggcgcggg acgggggatt tttccgtgaa
     2641 gccgatgtcc cgtcgtgcct ctaccttttt tttgcttata tcctggtttc ggcgcttaaa
     2701 aatttttgtt tcgcttcaaa gataatggcc acacccggtt tgaaacaatg tgttgttttt
     2761 caaggtgcaa gccgcgctgc gttccccgct ttttccggca atcaattttt gaattatttt
     2821 ttacgttatt tctgttccga aaggaatatt gagttgcggg atgttgtaat cgctccggtg
     2881 gatgcgccga agacgtttcc ccctcacgca cggcctttga ggatggtcaa ctttgtcaaa
     2941 aagatccttt gcgcgctcac ccagggcaaa aatatcgttc cgcggggggc gatctgtgtc
     3001 tatgggtccc tgcatcatct taaaagcgtt gttctggagc
//
LOCUS       DAOSIO010000189         2992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_28709_length_2992_cov_3.406197, whole genome shotgun sequence.
ACCESSION   DAOSIO010000189 DAOSIO010000000
VERSION     DAOSIO010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28709_length_2992_cov_3.406197"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PK590_06855"
     CDS             complement(<1..457)
                     /locus_tag="PK590_06855"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG24351.1"
                     /translation="MRVKETQFWSIRSKKYDNLEWANKGDYLHTFLNAGQFHEDDVAL
                     DVGSGTGIIAHTVSPFVKQVVGIDISEDMLKCAHNPAFKNVQFIRMDAHDLKFRTASF
                     HKVTARMCFHHIIEHTQKAMHECYRVLKKGGRMIFSEGVPPTKHVVPFYT"
     gene            complement(472..1998)
                     /locus_tag="PK590_06860"
     CDS             complement(472..1998)
                     /locus_tag="PK590_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOG24352.1"
                     /translation="MTSTVKSEVFRDTAKYISSQYLAQFFGFIISFAMKRFLGPSMIG
                     IWNVVLLIISYSSFAHLGTVEVAYRDIPFYKGAKQLEKAERLKNEAFTFSMIASIVPA
                     ICIVLYALCFKDSFKTPNLFLALLYAAVLMICQRAYDYYTTLLRANKAFTIISQVTIL
                     AAACNLILTFLLVWPFKLYGLFAASLLNVFLPFWFVSSKARYRISWTLSRDGLVAMLK
                     IGFPLFLLGLCSMTVSSIDKIIIAKYLGVTALGNYSIALMAGSYLLGMPNMFCIALYP
                     RLQEKYGEEQSPEGIKNYLLQPIQVVAVMIGFLVGLSVLTVPCLILNFLPEFASGLPA
                     MKIFVWSVFFFAIGQHAYTFLVTLNKQLKTIPIVLFGIAAIGGVGLFFVRLGWGIEGV
                     AGGVLVGSILYNLALIAYAKSYFASMRDSIRFFIFIIFIFVYFLFLTLSLASFGRDAS
                     FLDAFLRSALYLLIALPLFWILQKKTGFLILAFTIIRDKLKSAPCIVAERNTPKNSFN
                     "
     gene            complement(1982..>2460)
                     /locus_tag="PK590_06865"
     CDS             complement(1982..>2460)
                     /locus_tag="PK590_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24353.1"
                     /translation="SNGPIKIGDNCEIRDFTRIYAKHITVGNNVTLGEGVLLNGNIHL
                     KDGAWIARNCDLTGSVSIEKAILGPHVSCVGGSDHAREPVTGLVTMDGENAGDPTICI
                     LEGSWVGQGAIVLKGVVIGRNAIVGAGAVVTRNVEEGITAVGNPARKIRRKNDQYS"
     assembly_gap    2461..2470
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2677..>2992)
                     /locus_tag="PK590_06870"
     CDS             complement(2677..>2992)
                     /locus_tag="PK590_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24354.1"
                     /translation="SYLSTAIIECGIMNKTVLYYEPFPSGSAVASGYEKAGLCKIVYT
                     PQDLRQSIQSCLTSSTRMPVKAASEREYYLGLNDGLGTKRVVNHIMNELHGKNTHRDL
                     NL"
BASE COUNT          918 a          722 c          608 g          734 t
ORIGIN      
        1 cggtataaaa cggaacgaca tgcttcgtcg gcggaacccc ctcggaaaaa atcatccgtc
       61 cgcctttttt caaaacccgg taacattcat gcatcgcctt ttgcgtgtgt tcgataatgt
      121 gatgaaaaca catgcgggcg gtcactttat ggaaggaggc agtgcgaaat tttaaatcat
      181 gcgcatccat tcggataaat tgaacatttt tgaaagccgg attgtgggcg cacttaagca
      241 tgtcttcgga aatatcgatc ccaacgactt gcttgacgaa cggcgagacg gtatgcgcaa
      301 taatcccggt ccccgacccg acgtccaggg ccacatcatc ttcatggaac tgccccgcgt
      361 taaggaacgt gtgaagataa tcgcccttat tcgcccattc gagattatcg tacttctttg
      421 aacgaatact ccaaaattga gtttctttta ctcgcatcac aattcccttt cttaattaaa
      481 gctgtttttg ggtgtattgc gttcggcaac gatacaaggc gctgacttta atttatctct
      541 aatgatcgta aaagccagta ttaaaaaccc tgtttttttt tgaagaatcc aaaaaagagg
      601 gagcgcgatc aaaagatata aagccgacct cagaaaggcg tcaagaaaag acgcgtctcg
      661 accgaaggaa gcaaggctga gcgtcaaaaa taagaaataa acaaagataa aaatgatgaa
      721 gatgaaaaac cggattgagt cccgcatgct cgcgaaataa ctttttgcgt aagcgatcag
      781 cgctaaattg tataagatgg atccaacaag aacgccaccg gcaacgcctt ctatccccca
      841 ccctaggcga acaaaaaaaa ggcctactcc tcctattgcg gcgattccga acaacacgat
      901 cgggatcgtc ttcagttgtt tgttaagcgt gactaaaaaa gtgtaagcat gttgaccgat
      961 ggcaaaaaag aaaacactcc agacaaagat cttcatcgca gggagtcctg aagcgaattc
     1021 cggcaaaaaa ttcaggatca gacagggtac cgtgagcact gaaagcccta caagaaatcc
     1081 gatcatgacc gccaccacct gaatgggttg aagcaggtaa ttcttgatgc cttcgggaga
     1141 ctgttcttca ccatactttt cctgaagccg cggataaagc gcgatacaaa acatattggg
     1201 catcccaagg agatagcttc cggccatcag tgcgatcgaa taattgccca aggccgtcac
     1261 accgagatat ttggcaataa tgatcttatc aatactcgat accgtcatcg aacaaagccc
     1321 cagtagaaaa agcggaaaac caatcttaag cattgcaaca aggccatctc tcgaaagcgt
     1381 ccaactaatc ctatatcggg cttttgaaga aacgaaccag aacggcaaaa agacatttaa
     1441 aagcgaagca gcaaaaagtc cgtacagttt aaaaggccat actaacaaga atgtcaaaat
     1501 gaggttgcaa gcagccgcca gaatcgtcac ttggctaatg atggtgaatg ccttattcgc
     1561 tcgtaaaagc gtcgtgtaat aatcataggc cctctggcag atcataagaa cggccgcata
     1621 caaaagcgct aaaaataaat tgggtgtctt gaacgaatcc ttgaaacata aggcatagag
     1681 gacaatacaa atcgcgggaa caattgaagc gatcatggaa aaggtaaacg cctcgttttt
     1741 taaccgttcg gccttctcga gttgtttggc ccctttgtaa aagggaatat cacggtaagc
     1801 gacctcaaca gttccgagat gggcaaaact actgtaagaa ataatgagca acacgacatt
     1861 ccaaataccg atcatggagg gccctagaaa ccgtttcatg gcaaaactaa taatgaaccc
     1921 aaaaaactgt gccaaatatt gactggaaat gtattttgcc gtatcccgaa aaacctcact
     1981 tttaactgta ctggtcattt ttcctccgga tctttctagc aggattgcca accgcggtga
     2041 ttccttcttc cacgtttcga gtgacaacag cgcctgctcc cacgatagcg ttgcgtccga
     2101 tcacgactcc tttcaaaaca atagcacctt gcccgaccca tgaaccctcc aaaatgcaga
     2161 tcgtcggatc gcccgcattc tctccatcca tggtaactaa gcccgttaca ggttcacgcg
     2221 catgatctga gccgccaaca cacgacacat gcgggcccaa gatggctttt tcgatggaaa
     2281 cagatcctgt caaatcacaa tttcgggcta tccacgcccc atctttaaga tgaatattgc
     2341 cattcagcaa aacgccttct ccaagcgtaa cgttgtttcc aacggtaata tgtttcgcat
     2401 aaatccttgt gaaatcccgg atttcacaat tatctccgat ttttattggc ccattggatc
     2461 nnnnnnnnnn ccatccagca cgacatgacg tccaataaaa caatttttcc caatgtggat
     2521 attttttcct ccagaaatat gcgttgcgcc cacgacccgc aatccgttgc tcgcatgaga
     2581 cacgcttaat cggagaaata cagctcgaaa aaaaatcaat gattcttcct attcccttac
     2641 cgactgtcgc aacttttgta tgttttgtaa gatccgtcat aaatttaagt ccctatgagt
     2701 atttttacca tgaagttcat tcataatatg atttacaact cgcttggttc ccaagccgtc
     2761 attcaagcca agataatatt ccctttcaga agcagccttg accggcattc gcgtagatga
     2821 tgtgagacaa gactgaatgc tttggcgtag gtcctgggga gtgtaaacaa ttttacataa
     2881 tcccgccttt tcataccccg aggcaacagc acttccacta ggaaaaggtt cataatataa
     2941 gacggtttta ttcatgatcc cacattcgat aatagcggtt gataagtaac tg
//
LOCUS       DAOSIO010000190         2967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_28986_length_2967_cov_4.277129, whole genome shotgun sequence.
ACCESSION   DAOSIO010000190 DAOSIO010000000
VERSION     DAOSIO010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28986_length_2967_cov_4.277129"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1542)
                     /locus_tag="PK590_06875"
     CDS             complement(<1..1542)
                     /locus_tag="PK590_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOG24355.1"
                     /translation="MGETGKDAGVDIKDLLVRENIAKREDVEWAEKEILKNPRKQVVD
                     ALLEAGRCHEKKLASLMSALYGYRLVSLKMLVIHEDILALIPPKIAEAYSCIPVSILD
                     KTLTVAFANPTNLNAIDEVQIVTGKRVRAAVAEYSVLKECIRKFYFAGQKEDPVSEND
                     KEMDPESDELVCLAKQVREDSSETQQEETADLMAMASQAPVVKLVNMVLLEGIRRKAS
                     DIFIEPWENYVRVRIRVDGILEEIVKPQKSFGAAVVSRIKIMSHLNIAEHRVPQDGRF
                     KVRTQGREVDMRVSVLPSSFGEKVCLRILDTGSQAHDISKLGFLEKEQQLIRDCAKKP
                     HGMILVTGPTGSGKTTTLYSILNYLDSPQVNITTVEDPVEYQIPGINQVNVRESIGLT
                     FPVALRSILRQDPDIVLIGEIRDAETLDIAVKAALTGHLVLSTLHTNDSVSSITRMVN
                     LGLEPFLIASTVLMISAQRLVRKLCPRCKYAYDLEPEMYPILGLDPAKKIQLWRAKGC
                     SQCRKM"
     gene            complement(1558..2001)
                     /locus_tag="PK590_06880"
     CDS             complement(1558..2001)
                     /locus_tag="PK590_06880"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24356.1"
                     /translation="MTDKTNKKIGEILIEDGLIQPAQLAEALEYQKLNGGLIGQILIK
                     QKFLDEDSLVSALGKQARVPYIPLCRYAMNPEMATLLKADFCNTNLVVPFDGDARKVF
                     LAMADPLDQATVEKVQAMTGRAPKVYISRISEILNTIFYLYHEKK"
     gene            complement(2125..2880)
                     /locus_tag="PK590_06885"
     CDS             complement(2125..2880)
                     /locus_tag="PK590_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HOG24357.1"
                     /translation="MKPSKNIRWPRLTRAGCANFSSPKRKKNEKRAYGPTSKIKAEDL
                     PRIYHKLRKTFGHQSWWPAETPFEVMIGAILTQNTSWTNVEKAIANLRKAGLLSFSKM
                     KKLSHGQLARMIRPAGYYNVKADRIRSFLRFFQLEYQGSVLKMRREDLPTAREKLLRV
                     KGIGPETADSILLYALGKPTFVIDAYTKRIFSRHRLAKADNSYDEWKGIFEKALPAKT
                     PLFNDYHAQIVRLAKEFCRTRPRCEGCPLGDAT"
BASE COUNT          724 a          796 c          667 g          780 t
ORIGIN      
        1 catcttccgg cattgcgaac aacctttcgc gcgccagagt tgtatttttt tagccgggtc
       61 caaaccaagg attggataca tttcaggttc aagatcgtag gcatatttac agcgtgggca
      121 caattttctg acaagcctct gtgccgaaat cattaacacg gttgacgcga tcaagaaagg
      181 ctccagcccc aagttcacca tgcgcgttat cgagctgaca gaatcatttg tatgcagcgt
      241 actcagcaca agatgtcccg tcagcgcggc tttgaccgca atatccaaag tttccgcgtc
      301 gcggatctcg ccaatcaaaa caatgtctgg atcctgacgg agaatggagc gcaaagccac
      361 tgggaacgtc aaaccaatcg attccctgac attcacctga ttgatccccg ggatctggta
      421 ttccacgggg tcttcaaccg tcgtgatatt gacctgggga gaatcgagat aatttaaaat
      481 cgaatacaaa gttgttgttt tcccgctccc ggtgggaccc gttaccagga tcattccgtg
      541 aggttttttc gcgcaatccc gtatcagttg ctgctctttt tcgaggaaac cgagtttcga
      601 aatatcatga gcctgagagc ccgtatcgag aattcgaaga caaacttttt cgccaaagct
      661 cgacggcaga accgaaacgc gcatgtccac ttcacgcccc tgggtccgca ctttaaagcg
      721 gccatcctgc gggacacgat gttcagcgat gttgagatga ctcatgattt tgatccggga
      781 aaccaccgcg gccccaaaac ttttttgagg tttcacaatt tcttccagaa tgccatccac
      841 gcgaatccgg acacgaacat agttctccca gggttcaata aatatatcgc ttgcttttct
      901 tcgaatccct tccaggagca ccatattcac aagtttgacg acgggggcct gggatgccat
      961 cgccatgaga tccgcggtct cctcctgctg ggtttctgaa gaatcctctc tgacctgttt
     1021 cgcgaggcat accagctcat ccgattccgg atccatttcc ttgtcgtttt ccgaaacagg
     1081 gtcttctttc tggcccgcaa aataaaattt ccggatacat tccttgagga cagaatattc
     1141 cgcgaccgcg gcccgaactc tctttcccgt gacaatctgc acttcatcaa tagcattcag
     1201 atttgtggga ttggcgaagg caaccgtgag tgttttatcc aaaattgaaa cgggaataca
     1261 cgagtaagct tcggcaattt tggggggaat cagcgcgagg atatcttcat gaatgacaag
     1321 catcttgaga ctgaccagcc ggtagccata aagcgcgctc atcagactgg caagtttttt
     1381 ttcgtggcat cgccccgctt ccagaagcgc atccaccacc tgtttacggg gatttttcag
     1441 gatttctttt tcggcccact caacatcttc acgcttggcg atgttttcgc gcaccagcaa
     1501 atccttgatg tcaacccctg catctttacc cgtttcaccc atagaaaaac agcctcatta
     1561 ttttttctcg tgataaagat aaaaaatggt attcagaatt tcggaaattc gcgaaatata
     1621 aaccttgggc gcccggcccg tcatggcttg aaccttctcg acagtcgcct gatccagggg
     1681 atccgccatc gcgagaaaca ccttcctggc atctccatcg aaaggcacga ccagattggt
     1741 attacaaaaa tccgccttta aaagcgttgc catttcggga ttcatcgcgt aacgacagag
     1801 cgggatgtaa ggcacacgcg cctgtttccc gagagcgctc acaagactgt cctcatcaag
     1861 gaacttctgc ttgattaaaa tctgcccgat taatcccccg ttcagttttt gatactcgag
     1921 ggcttcggca agctgtgccg gttggatcag accgtcttcg atcaggattt ccccaatttt
     1981 tttatttgtt ttatccgtca taaaatccac tcccgtcaat acaccctaag taacgacata
     2041 tttttaaaag agctttgcta ctttttaaaa aatttacgtc cccttcaggg tgaagcgggt
     2101 cggaaaaact ccccgccccc tcaatcacgt agcatccccc aacgggcatc cttcgcatct
     2161 cggccgagtc cggcaaaact ctttcgccag ccggacgatc tgggcatggt aatcgttaaa
     2221 aaggggcgtc tttgccggaa gagccttttc aaaaatcccc ttccattcat cgtaagaatt
     2281 gtccgctttt gcgagccgat gacgggaaaa aattctcttg gtatacgcgt ctatcacaaa
     2341 agtgggcttg cccagagcgt aaagaagaat cgagtccgcc gtctccgggc caattccctt
     2401 cacgcgaaga agtttctctc tggccgtcgg aagatcttcc ctccgcattt tgaggacact
     2461 tccctgatat tccaactgaa agaatcttaa aaaactcctg atccgatccg ctttgacgtt
     2521 gtagtacccg gcggggcgaa tcattcgagc caactggccg tgagagagct tcttcatttt
     2581 tgaaaaggac agcagacccg cttttcgcaa attggcaatc gccttttcca cattcgtcca
     2641 ggatgtgttc tgggtcaaaa tcgcgccgat catgacctca aaaggcgtct ccgcgggcca
     2701 ccacgactga tggccgaacg tttttctgag cttgtgataa atgcgtggca gatcttccgc
     2761 tttaattttg gaggtggggc cataagcccg tttttcattt ttcttcctct tcggactgga
     2821 aaaattcgcg caaccggctc tagtcaatcg cggccatcgg atattctttg agggcttcat
     2881 aaatcgctcg cgaaggttga agttcacttt tgaaaagcac catctttttc aatggcttca
     2941 gcgaggtagg caatggaagt ggccgac
//
LOCUS       DAOSIO010000191         2909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_29730_length_2909_cov_2.842677, whole genome shotgun sequence.
ACCESSION   DAOSIO010000191 DAOSIO010000000
VERSION     DAOSIO010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29730_length_2909_cov_2.842677"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PK590_06890"
     CDS             <1..295
                     /locus_tag="PK590_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOG24358.1"
                     /translation="LAMRYKEAFNNFSDVHFVDEQAHGKSNFWLNAIMLDAELAGERD
                     AVLGHLRSNGIMARPAWTLMHKLPMFQDCPRMDVPVAEDVESRLINIPSSAFL"
     assembly_gap    387..396
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <397..1438
                     /gene="neuC"
                     /locus_tag="PK590_06895"
     CDS             <397..1438
                     /gene="neuC"
                     /locus_tag="PK590_06895"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020784366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HOG24359.1"
                     /translation="ATGTHLSAVFGSTYRDIERDGFTIAKKIPILTSDDSALGVTRSL
                     GKAVTGFATAFRDLKPDWLVVLGDRYEILAAVEAALIAKIPVAHLAGGDITEGAVDDS
                     IRHAITKMSHLHFATHAASARRIRQLGEDPKTIFCPGHIGLDGIRNTDFLSRFELEKS
                     LRFKFRQRNFLITFHPATLDRIPSLRQLDELLSALGRLGTDTGLIFTASNADREGIEM
                     NRKIKSFVSTRDNTCFIASMGQRRYLSAMKYVDVMVGNSSSGIYEAPSFKLPTVNIGD
                     RQRGRQQATSVINCPPQASRIYEAILKALKKNCSKVTNPYGDGRSAPRILRAIKKIRD
                     PQGLLKKKFCDI"
     gene            1440..2525
                     /gene="neuB"
                     /locus_tag="PK590_06900"
     CDS             1440..2525
                     /gene="neuB"
                     /locus_tag="PK590_06900"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HOG24360.1"
                     /translation="MKNKKTTYVIAEAGVNHNGSVRLAKKLIDVAANAGADAVKFQTF
                     RAEALVTAAMPKAVYQRRDRASGSTQFEMLKKLELSDRDHKELYRHAEKRGIEFLSTP
                     FDETCLDFLVELGVKKIKIPSGEITNGPLLLATARTGLPVILSTGMANITEVEDAVSI
                     LSFGYISPNLPVRSLQKAKVVFRNSEAKSIVRKKVTLLHCTTEYPALDRDLNLAAMNS
                     LRRHFGCRVGFSDHSKGIMVAVAAVALGAAVIEKHLTLDKKMIGPDHAMSLEPDEFAE
                     MVRGIRTVESAIGHGVKRPVCSEMKNIAVVRKVLVASQPIHKGEFFTPENLTAKRAGK
                     GVSPMLYWDILGKKAKRDFQTDQMVTL"
     gene            2522..>2909
                     /locus_tag="PK590_06905"
     CDS             2522..>2909
                     /locus_tag="PK590_06905"
                     /inference="COORDINATES: protein motif:HMM:NF036822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar acetyltransferase"
                     /protein_id="HOG24361.1"
                     /translation="MNRPVIVLGGGGHARVLIDALRLSKNRILGVTDFRPSSVNPQKV
                     GVKILGDDGAVLSFSPAKVSLANGLGSTGSTAARMALFEKFKVKGYRFISVIHPSAVI
                     AKDAVLGEGVQIMAGAVIQTGVVIGNN"
BASE COUNT          728 a          647 c          780 g          744 t
ORIGIN      
        1 cctcgcgatg cgttacaagg aagcgtttaa taattttagc gatgttcatt ttgtcgatga
       61 gcaagcgcac ggcaaaagta atttctggtt gaatgccatc atgctggatg ccgagttggc
      121 cggagaacgt gatgctgtgc tcggccactt gcgttcgaat ggtatcatgg cccgtcctgc
      181 gtggacgctt atgcacaagt tgccgatgtt tcaggattgt ccgcgcatgg atgttcccgt
      241 cgccgaagat gtggaatcgc gtctcatcaa cattccgagc agtgctttcc tatgaagaaa
      301 cgaaagatct gcgtcgttac gggctcccga gcggaatacg gtctccttta ttggctgatt
      361 agggagattt gcaaagacca ttctctnnnn nnnnnnagcg accggaacgc atctttccgc
      421 agtttttggc tcgacctatc gcgatattga aagggacggt tttacaattg ccaagaaaat
      481 cccgatcctg acttctgatg attctgcgtt gggcgtcaca cgatcgctcg ggaaagcggt
      541 tacaggcttt gcgacggcct tcagggattt aaaacctgac tggctggttg tgctcgggga
      601 tcgctatgaa attttggcgg cggtcgaagc ggctttaatt gccaagatac ctgtggccca
      661 tcttgcggga ggggatatca ccgaaggtgc ggtcgatgat tctatacgcc atgcgattac
      721 aaaaatgtct catcttcatt tcgcgacaca tgcggcatcg gcgcgaagga tacgtcaact
      781 gggagaagat ccaaaaacga ttttttgccc gggacatatc ggtcttgacg gaataaggaa
      841 tacggatttt ttaagtcgct tcgagcttga aaaaagtttg cggtttaaat tccggcaaag
      901 gaacttttta attacattcc atcccgcaac attggacaga atcccgtccc tgcggcaact
      961 ggatgaattg ctttctgctc tggggcgcct aggaaccgac acgggtttga tctttaccgc
     1021 ctctaacgcg gatcgtgagg ggattgaaat gaatcgaaag attaaaagtt ttgtatccac
     1081 ccgcgataat acatgcttta tcgcttcgat gggacaacga cgttatttga gcgctatgaa
     1141 gtatgttgat gtcatggtag gaaattcatc aagcggcatt tatgaggctc cctcttttaa
     1201 actccccact gtgaatattg gggatcgcca acgaggtcgc cagcaggcta cgtctgtgat
     1261 caattgtccc cctcaagctt cccggatcta tgaggcgatc cttaaagctc tcaaaaaaaa
     1321 ctgctcgaaa gttactaacc cctacggtga tggccgctcg gcgccgagaa tcctccgcgc
     1381 gataaaaaaa atcagggacc cgcaagggtt acttaagaag aaattttgtg atatctaata
     1441 tgaaaaataa aaaaacaacc tatgttatcg ctgaagcggg ggtgaaccat aacggttccg
     1501 tgcggctcgc caaaaagctg attgatgttg ccgcgaatgc cggcgctgac gccgtaaaat
     1561 ttcagacttt tcgcgctgag gccctcgtca ccgcagcaat gccgaaagca gtctatcagc
     1621 ggcgtgaccg ggcgagcgga tccacgcagt ttgagatgtt gaagaagctg gagctgagcg
     1681 accgggatca taaggaactc tatcgccatg ctgaaaaacg cgggatcgag tttttatcga
     1741 ccccgttcga tgagacatgt ctcgattttc tcgttgagct cggcgtgaaa aagatcaaga
     1801 tcccttcggg ggagattacg aatggtccgc tcttgttagc aacggcaaga acaggtttac
     1861 ccgtgatact ttcaaccggg atggcaaata ttacggaagt ggaagatgct gtgtcgatcc
     1921 tttcttttgg atatatctct ccgaacttgc cggttcgttc actccaaaag gccaaggtag
     1981 tttttcgaaa ctccgaagca aaatcaatcg tgagaaaaaa ggttacttta cttcattgca
     2041 cgacagaata tcctgctttg gatcgtgacc tgaacttggc ggccatgaat agcttacgac
     2101 gtcattttgg atgcagggtt ggtttttctg atcatagtaa ggggataatg gtcgctgtgg
     2161 cagcggttgc cctgggggcg gcggtcatcg agaagcattt gactttggat aaaaaaatga
     2221 taggaccaga tcatgcaatg tctttagagc cggatgaatt tgctgaaatg gtgcgaggta
     2281 ttcgaaccgt tgaatctgcg atcggccatg gtgtaaagcg acctgtctgt tcggaaatga
     2341 aaaatatagc ggttgtgcgg aaggttttgg tggcttccca acccattcat aaaggagaat
     2401 tttttacgcc cgagaatctg actgcaaaac gtgccgggaa gggagtttcg ccgatgttgt
     2461 attgggatat tttaggaaaa aaagcgaagc gtgatttcca aaccgatcaa atggtgacat
     2521 tatgaacagg ccggtcatcg tactcggtgg cggcggacac gcacgcgtct tgatcgacgc
     2581 tttgcgactc agtaaaaacc ggatactcgg tgttacggat tttcggccat cctcagtcaa
     2641 tccccaaaag gttggcgtta aaattctcgg agacgatggg gcggttcttt catttagtcc
     2701 cgcgaaggtt tccctcgcca atggactggg ttccaccggg tcgacagcgg ctcggatggc
     2761 gttgtttgag aaattcaagg tgaaaggcta ccggtttatc tcggtgatcc atccctccgc
     2821 ggtaatcgca aaagacgcgg ttttgggaga aggtgtacaa atcatggcgg gcgcggttat
     2881 ccaaaccggg gttgtgatcg ggaacaatg
//
LOCUS       DAOSIO010000192         2896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_29893_length_2896_cov_3.727561, whole genome shotgun sequence.
ACCESSION   DAOSIO010000192 DAOSIO010000000
VERSION     DAOSIO010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29893_length_2896_cov_3.727561"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PK590_06910"
     CDS             <1..606
                     /locus_tag="PK590_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HOG24362.1"
                     /translation="DLIVVDSVAALVPKAEIEGEMGQAQMGLQARLMSQAMRKLTAII
                     SKSKTCIIFINQLREKIGVMFGNPETTPGGKALKFYASMRIDIRRIASLKRGDEIVGN
                     RVRVKIVKNKVAAPFRQAEFDILFSEGISRSSNIVELGTLYGVIEKSGAWLSYNNEKI
                     GQGKDAARLFLKENPKLLEEIERRILEKVAAGETAIVGAEN"
     gene            617..1114
                     /locus_tag="PK590_06915"
     CDS             617..1114
                     /locus_tag="PK590_06915"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOG24363.1"
                     /translation="MAQPPDEPSKQAIITALRLLAASPKSGHELKDKLVSKGYPPEAV
                     RFALNELGERGLIDDKVYANNLTRQLIHGKLSGKHKIAFELKRHGIPESIRKEILNSL
                     TESDEKERALEQARLKWAGFQSFDSKKRRKKIFDHLIRKGYDFQVVHEVMDEISKDPE
                     TDGLR"
     gene            1118..>2896
                     /gene="alaS"
                     /locus_tag="PK590_06920"
     CDS             1118..>2896
                     /gene="alaS"
                     /locus_tag="PK590_06920"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOG24364.1"
                     /translation="MSSHYSVDFIRKTFIDFFASKGHSVVASDSLVPQNDPSLLFTGA
                     GMNQFKEYFLGVKKDMKRAVTSQKCLRTGDLDEVGRTPYHHSFFEMLGNFSFGDYFKQ
                     EAITWAWEFLTLSLKIPAERLRVTVHENDREAFEIWNQRIGLPESWIYRCGDASNFWP
                     ANAPKEGPNGPCGPCSEIYFDQNPSIQGTPVDSSGKFTEIWNLVFTQFDRKDGGHLEP
                     LANKNIDTGMGLERLACVLQGKTSNFEIDSFQPVHSAILKALELTSAGSGTKPLYCIA
                     DHLRAIVISISDGVVPSNEGRGYVIRKLIRRAIWQAHQLNPKVKRPFLFQAMDGVLTA
                     MKSAYPELLDAEENVRMVIKQEEERFLKTLESGLKILTETIAEHQREGRTALSAQSVF
                     ELYDTYGFPEELTRMIAEEKNISIDYAGVEKLMEDQRLRSKESSKISGDIFSKSELEE
                     IPSHLPGTRFLGYETLDANAKILWFQREGSQATVILDQTPFYAESGGQLGDQGMLESP
                     TVTIKVTDTKKLDKYFLHQGEILRGNPETGETVEAKVDSLRRAAIMRNHTATHLLHAA
                     LRHHLGVSVRQLGSLVAPDRLRFDYSF"
BASE COUNT          789 a          665 c          733 g          709 t
ORIGIN      
        1 gatctcatcg tcgtcgactc ggtcgccgct ttggttccca aggccgaaat tgaaggtgag
       61 atggggcaag ctcagatggg gttgcaggcc cgtttaatgt cacaggcgat gagaaagttg
      121 acggccatta tcagcaaatc gaagacttgt attattttca tcaatcagct tcgcgaaaaa
      181 attggcgtga tgttcggaaa ccctgaaaca acaccgggcg gaaaggcgct caagttctac
      241 gcctccatgc gaattgacat tcgccggatc gcgagtctta aacgaggcga tgagatcgtc
      301 ggtaaccgcg tgcgggtcaa gattgtcaaa aataaggtgg ccgcgccctt ccgtcaggcg
      361 gagtttgaca ttctcttctc ggaaggtatt tcacgctcgt ccaatatcgt ggaacttgga
      421 actctgtatg gcgtgatcga aaaatcgggc gcctggcttt cttataacaa tgagaaaatc
      481 gggcaaggga aagatgccgc ccgtcttttt ctgaaagaaa atcccaaatt actcgaagag
      541 attgaacgaa gaattctcga gaaggttgcc gcgggcgaaa ccgcgattgt tggcgcggaa
      601 aactgatcgc cgcgtcatgg cgcaaccgcc ggatgaacca tccaaacagg caattatcac
      661 ggctcttcga ttactcgcag ccagtcctaa aagcggacac gaacttaagg acaagttggt
      721 ttccaaagga tatcctcctg aggcggttcg ctttgccctt aacgaactgg gcgagcgggg
      781 actgattgat gataaggtct acgccaataa tttgacccgt cagctgatcc atgggaaatt
      841 atccggaaaa cataaaatcg cgtttgaatt gaagcgtcac ggtattccgg aatccatccg
      901 gaaagaaatt ctgaattcat taacagaatc ggatgaaaaa gaacgcgcct tggaacaagc
      961 ccggctaaaa tgggcgggtt ttcagtcttt cgattctaaa aagagaagaa aaaagatttt
     1021 tgatcattta atcaggaaag gatatgactt tcaagtcgtg catgaggtca tggatgagat
     1081 tagcaaggat cctgaaactg acggtctgcg ataagatatg agctcccatt attcagtcga
     1141 ttttattcga aaaacgttta tcgatttttt tgcttccaaa ggacattccg tcgtcgcaag
     1201 cgattccctt gttccgcaga atgatccctc tctgcttttt accggggcgg gaatgaacca
     1261 gttcaaggaa tacttccttg gcgtcaaaaa agacatgaaa agggccgtaa ccagtcagaa
     1321 atgcctccgg acgggcgatc ttgacgaggt tggaaggaca ccctaccatc attctttctt
     1381 tgaaatgctc ggaaattttt cattcgggga ttacttcaag caagaagcaa taacctgggc
     1441 ttgggaattt ttgactttgt cgctgaagat tccggcggaa cggcttcgtg taaccgttca
     1501 cgagaatgac cgggaagcct ttgaaatctg gaatcagcgg atcggtctgc cggagagctg
     1561 gatctaccga tgcggagatg cttcaaattt ttggccagcc aatgcgccta aagaagggcc
     1621 caacggcccc tgcggcccat gttcggaaat ttatttcgac caaaatccat caatccaagg
     1681 cacgcccgta gattcatcgg gtaagttcac cgaaatatgg aatttggtat ttacccaatt
     1741 tgatcggaaa gacggtggac accttgaacc ccttgccaac aaaaacatcg atacaggaat
     1801 ggggcttgaa cgactggcct gcgtccttca aggaaagact tccaattttg aaatagattc
     1861 ttttcagccc gtgcacagcg cgatcttaaa ggcgcttgaa ctaacatcgg ctggttctgg
     1921 cacgaaacct ctttattgca tcgcggatca tcttcgagcc attgtaattt cgatttcgga
     1981 cggtgttgtt ccctcgaatg aaggacgcgg ttatgtcatt cgaaagttga ttcgtcgcgc
     2041 gatttggcag gcgcatcaac ttaatccaaa ggtcaaacgt cctttccttt tccaggcgat
     2101 ggacggagta ctaacggcca tgaaatccgc ctacccggag cttctggacg ccgaagaaaa
     2161 tgtgcggatg gtcattaaac aagaggaaga acgtttttta aaaactctcg aaagtggact
     2221 taaaattctt acggaaacta tcgcggagca ccagcgggaa ggacggacag ctctctccgc
     2281 ccagtcagtg tttgaattgt atgataccta cgggtttccg gaagaattga ctcgcatgat
     2341 cgcggaagaa aagaatatct cgatagatta cgcgggggtc gaaaaactga tggaggatca
     2401 aaggttgaga tccaaggagt cgagcaaaat ttcaggtgac attttttcga agtcggaact
     2461 tgaagaaatt ccgtctcatc ttcccggaac ccgattcttg ggatatgaaa cgctggacgc
     2521 aaatgccaaa atcctttggt ttcaacgcga agggagccag gccacagtga ttcttgatca
     2581 aacacctttt tatgccgaaa gcggtggtca gctcggagat cagggaatgc tcgaatcccc
     2641 gacagtcacg atcaaagtta ctgacacgaa gaagcttgat aaatattttt tgcatcaagg
     2701 ggaaattctt cgtggaaacc ctgaaacagg ggaaaccgtt gaggctaagg ttgattctct
     2761 ccgccgcgcg gccatcatgc gaaaccatac cgcgacccac ctcttgcatg cggcgcttcg
     2821 tcatcatctg ggagtgtctg tccgccaact tggttccctg gttgcgccgg accgcttgag
     2881 gtttgactat tcgttt
//
LOCUS       DAOSIO010000193         2875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_30175_length_2875_cov_3.927305, whole genome shotgun sequence.
ACCESSION   DAOSIO010000193 DAOSIO010000000
VERSION     DAOSIO010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30175_length_2875_cov_3.927305"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PK590_06925"
     CDS             complement(<1..340)
                     /locus_tag="PK590_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG24365.1"
                     /translation="MIARADGGSTRAKKTKRVLAIDDDGLVRKSLEIFLKEAGYEPTV
                     AESGEEALRILSGRHFDLLVTDIRMPGMDGLQVIQAVREYCRQMRKNPIPEIVLTAYN
                     DEHVKQSVERL"
     gene            complement(337..909)
                     /gene="efp"
                     /locus_tag="PK590_06930"
     CDS             complement(337..909)
                     /gene="efp"
                     /locus_tag="PK590_06930"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOG24366.1"
                     /translation="MIIATDIRVGHVIQLDGKLSKVVTREIRGTGKFGKTVHCKMRNL
                     EDGNVIEKSFRAEDKVEDVPVRVIKMQYLYREGEQFVFMNMENYEQYTISSKAVGRHE
                     VFLKENETIEVLFGQGKVLSLNFPKLVELTVTSTAPGVKGQQDTTYKEAELENGLKIL
                     VPQFIREGEKIRVHTEDLSYYERVTVRSMS"
     gene            complement(1001..1510)
                     /gene="cyaB"
                     /locus_tag="PK590_06935"
     CDS             complement(1001..1510)
                     /gene="cyaB"
                     /locus_tag="PK590_06935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOG24367.1"
                     /translation="MTSFEIEQKYRVKRPGEIRSILKKIKAKKIASGKEINEFFDHRG
                     SLRSKKIALRVRQYDGKAVLTLKGPRLRSAYTKRMELEMSVDYHSIRRILTLSGLKPF
                     MKYQKFRELYRLGKADIALDKLNRFGWFLEIEGTVREISRIAKALGLKNSDRERRSYL
                     GMLFNWKCS"
     gene            complement(1517..2071)
                     /locus_tag="PK590_06940"
     CDS             complement(1517..2071)
                     /locus_tag="PK590_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOG24368.1"
                     /translation="MAKTAIVLLAEGFEEIEALAPVDVLRRAGIRVTIAGVDASTVKS
                     SRGVSVGTDMLLDQVIELSDAVIVPGGMPGSTNLARSEKVSEFLKKMNGSGKLIAAIC
                     AAPAVVLAPLGILDGKKATCYPGCEADFTPGTRYSAESVVTDGNVITSQGPGTALAFS
                     LAIVEALAGTAMVKTLREKMLIGV"
     gene            complement(2071..2529)
                     /locus_tag="PK590_06945"
     CDS             complement(2071..2529)
                     /locus_tag="PK590_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24369.1"
                     /translation="MLLPVPKTNAMLICDYVITERGTNKKSLIGVFENIGATTFPCTH
                     FAMSVYVKLTDAQGTYRFRLDLVDLKEDKTVGQSEIPNDVQVPSPLVAHELVFNLRGV
                     RFAHPGEYEFRIFANGSIFGQKKFVVEQAVRKGPDGPPDFERPPQDGGGV"
     gene            complement(2594..2860)
                     /locus_tag="PK590_06950"
     CDS             complement(2594..2860)
                     /locus_tag="PK590_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010471158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG24370.1"
                     /translation="MKITVEKPTESKLRELDVKNWPIWMKEVSVFDWSYDEKEVCYFL
                     EGEVTVTTSQGDVSFGQGDLVTFPEGIQCVWRIKKAVKKHYRFG"
BASE COUNT          677 a          846 c          600 g          752 t
ORIGIN      
        1 ctaaacgttc gacggattgc tttacgtgtt catcattata agcggtgagc acgatctccg
       61 gtatgggatt cttcctcatc tgtcggcaat attcacgaac ggcttgaatg acctgaagac
      121 cgtccatgcc cggcatccgg atgtccgtca cgagaagatc aaagtggcga ccgcttaaaa
      181 tcctgagggc ctcttcaccg ctttccgcca cggtcggttc gtaccccgcc tctttcaaaa
      241 aaatctcgag ggattttcga accaatccat cgtcgtcaat agctaaaacg cgttttgttt
      301 ttttggcacg cgtcgagccc ccatccgccc tcgctatcac gacatactcc tgacagtcac
      361 gcgctcataa tagctcaaat cttcggtatg gaccctgatt ttttctccct cacggataaa
      421 ctgcgggaca agaatcttga gaccgttctc aagctccgct tccttgtacg tcgtgtcctg
      481 ctgtcccttg acaccgggcg ccgttgaagt cacggtaagc tccacgagtt tcggaaaatt
      541 gaggctcagc actttccctt gcccgaaaag cacctcaatc gtctcgtttt ccttgaggaa
      601 aacctcgtgc cgccccacgg ccttcgacga aatcgtgtac tgctcgtaat tttccatgtt
      661 cataaaaaca aactgctccc cttccctgta aagatattgc atctttataa cacgaacggg
      721 gacgtcctcg accttgtcct ccgcgcgaaa actcttttca atcacgttcc catcctcgag
      781 gtttctcatt ttgcaatgaa ccgttttccc gaacttcccg gtcccgcgga tttcccgggt
      841 caccactttg gaaagtttcc cgtcgagctg aatgacatgc cccaccctga tgtctgtcgc
      901 gataatcatg tcggacctcc tgtcttgttc attggccaaa ccctaaaggc cgtctggctt
      961 tcgttcatca ttaccatact ctggatccca aaaagctgtc tcacgaacat ttccagttaa
     1021 aaagcatgcc aagatatgag cgtctttccc gatcactgtt ctttaatcca agggctttcg
     1081 cgatcctgga aatttcgcga accgttcctt cgatctcgag aaaccatccg aaccgattca
     1141 gcttatcaag agcaatatcg gctttgccaa gccggtacaa ttcccgaaat ttttgatatt
     1201 tcataaacgg cttcaaaccc gaaagagtca gaatacggcg aattgaatga taatccacgg
     1261 acatttccag ttccatccgc ttggtgtacg cggagcggag ccttggccct ttcagggtca
     1321 aaaccgcttt tccgtcatac tggcgaaccc gcaacgcaat tttttttgat ctcaaagaac
     1381 ccctatggtc aaaaaactcg ttaatttctt tccctgaggc gatttttttc gcctttattt
     1441 tttttagaat cgaccggatt tcccccggcc gttttaccct gtatttttgc tcaatctcaa
     1501 aagaagtcac gagacattaa actccgatca gcattttttc acgaagcgtc ttgaccattg
     1561 ccgtaccggc aagcgcttcc acgatggcca gcgaaaacgc gagcgctgtc cccggcccct
     1621 gactcgtaat cacgtttcca tccgtaacga cggattccgc ggagtaacgc gtaccgggcg
     1681 taaaatccgc ttcgcaaccc ggataacagg tggctttttt tccatcaaga atccctaggg
     1741 gggcaagcac gacggcggga gccgcgcaga tcgccgcgat caatttcccg gatccgttca
     1801 ttttttttaa aaactcggag accttttcag agcgggcgag gttcgttgac cccggcattc
     1861 caccgggaac aatcaccgcg tcggaaagtt caatcacctg atccagtaac atatccgtcc
     1921 caacgctgac tccccttgaa cttttcacgg tagaagcgtc gacgccggca atcgtgacgc
     1981 gaattcccgc acggcgcaac acatcgacgg gcgccagggc ctcaatttcc tcaaaacctt
     2041 ccgccagcaa aacgatagcc gtctttgcca tcacacacct cctccgtcct ggggcggccg
     2101 ttcaaagtcc ggaggtccat cgggaccttt tcggaccgcc tgttctacga caaatttttt
     2161 ctgtccaaaa atggaaccat tcgcgaaaat cctgaactca tattcgcccg gatgcgcgaa
     2221 ccgaactcct cgcaggttaa aaacaagttc gtgcgccacc agcgggctcg gcacctggac
     2281 atcattcggt atttcgcttt gtccgacggt cttgtcctcc ttaagatcca cgagatccag
     2341 acggaaccga taggtcccct gcgcgtcggt caatttcacg tagactgaca tcgcgaaatg
     2401 ggtgcaggga aatgtcgtgg caccgatatt ctcaaaaacc ccgatcaggc tctttttatt
     2461 agtccccctc tcggtaataa cataatcaca aatcagcatc gcgttcgttt taggaaccgg
     2521 caaaagcatg gcaacgtcct ttccttaaaa attaaattcg tttcagcgtt aagttccatc
     2581 cccggagttc gtttcatccg aaccgatagt gctttttgac agccttttta atacgccaga
     2641 cgcattgaat cccttccggg aaggtcacaa gatctccctg gccgaaagac acatccccct
     2701 gcgaggttgt gaccgtgacc tctccttcca gaaaatagca aacttccttt tcgtcataag
     2761 accagtcaaa aaccgaaact tccttcatcc agatcggcca atttttaaca tcaagctccc
     2821 taagttttga ttccgtgggt ttttctaccg ttattttcag cgcggattgc tgtgt
//
LOCUS       DAOSIO010000194         2866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_30304_length_2866_cov_4.917467, whole genome shotgun sequence.
ACCESSION   DAOSIO010000194 DAOSIO010000000
VERSION     DAOSIO010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30304_length_2866_cov_4.917467"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..485)
                     /gene="nrdR"
                     /locus_tag="PK590_06955"
     CDS             complement(27..485)
                     /gene="nrdR"
                     /locus_tag="PK590_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOG24371.1"
                     /translation="MKCPFCKRDSDKVIDSRAANEGEVIRRRRGCTACGKRFTTYERV
                     EEIPMFVVKKDQRRESYDRQKVLSGVHKACEKRPVPLAAQDAIADDLENMIREKYEKE
                     IPSRMIGEFIMQRLAKVDQVAYVRFASVYRDFQDVSHFMKELKFLLAKPK"
     gene            complement(490..1758)
                     /gene="glyA"
                     /locus_tag="PK590_06960"
     CDS             complement(490..1758)
                     /gene="glyA"
                     /locus_tag="PK590_06960"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOG24372.1"
                     /translation="MNKDVTDYSHLKASDPEIYRAIRAEVGRQNENIELIASENFTSI
                     SVLETMGSVLTNKYAEGYPGKRWYGGCENVDVVERLAIDRAKQLFGAEHANVQPHSGT
                     SANIAVFMALLEPGDKILGMNLTHGGHLSHGHPMNYSGKFYTIIPYGVSPANEQIDYD
                     ELERLAHANRPKLILLGASAYPRVIDFERGKKIADSVGALLMVDMAHFAGLVATGHHP
                     SPVPYADIVTTTTHKTLRGPRAGLILCKEKYAKAIDSSVFPGIQGGPLMHVIAAKAVA
                     FKEAMSPAFKEYSGQIVKNAKVLADAMGRRGWRIVSGGTDNHLMLVDVTVKGFNGKQV
                     QEVLDKVKITVNKNMIPFDKESPFKGSGVRLGTPAVTTRGMKEPEMELIAELITETMK
                     APEDSGNLAKVRARVETLTKKFPLYPGLMG"
     gene            complement(1739..2194)
                     /locus_tag="PK590_06965"
     CDS             complement(1739..2194)
                     /locus_tag="PK590_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HOG24373.1"
                     /translation="MIKPRIAIACDHRGLQMKNALIDFLRKKNFEVVNCGTDTGESCD
                     YPDFIFQAAEKVAKKECHRAIGICYTGIGSAIAANKVCGVRAALAQNIEQARLSRAHN
                     DSNMLILGAGFLPQDILFPLVDSWLNTPFDRGRHEIRVKKIKTYEQGRH"
     gene            complement(2218..>2866)
                     /locus_tag="PK590_06970"
     CDS             complement(2218..>2866)
                     /locus_tag="PK590_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HOG24374.1"
                     /translation="SANQSGLLSPHTAHQVVEQLGSKIRLLIDTGRTEFSHDSTVLDV
                     SGEEPVILRKKAYGTEVEKAVARVKTDQYPKKKILVVCTGNSCRSPMAEGWLKKELEL
                     KGLSDEIEVASCGIGARDGLPAASEAEYVMRNRGVDLSCFKSRQCRDSDIWGADVILV
                     MAEHHADSVEELMPGSREKIQCLDVEDPIGLGMMVYEKTINQIEKKIRNIFHKIV"
BASE COUNT          678 a          836 c          602 g          750 t
ORIGIN      
        1 tacaataaga gctttactat agggaattat ttcggcttcg ccaagagaaa cttcagttct
       61 ttcataaaat ggcttacgtc ctgaaaatcg cgatagacac tggcaaaccg gacatacgcc
      121 acctgatcca ccttggccaa ccgttgcatg ataaattccc caatcatccg ggacggaatt
      181 tctttttcat atttttcccg gatcatgttc tcaagatcat ccgcgatcgc atcttgcgcc
      241 gcgagcggaa cgggtctttt ctcacaagct ttatgaacac cggataaaac cttttgccga
      301 tcatacgact cgcgacgctg atcctttttg acgacaaaca tcggtatttc ttccacgcgc
      361 tcataagtcg taaacctttt accgcaagcg gtgcaccccc ggcgccgacg tatcacttct
      421 ccttcattcg ccgctcggga atcgatcact ttatccgaat cccgcttgca aaagggacat
      481 ttcatttggt taccccatca gtcccgggta aagcgggaat tttttcgtga gggtttccac
      541 gcgcgcgcga actttggcaa gatttcccga atcctcaggc gccttcatcg tttccgtaat
      601 cagttcggcg atcagttcca tctcgggctc tttcatcccg cgggtcgtga ccgccggtgt
      661 tcccaagcgg actcctgatc ccttaaacgg agactcttta tcaaacggga tcatgttttt
      721 attcaccgtt atcttcacct tatcaagaac ttcctgaacc tgttttccgt tgaatccctt
      781 gaccgtcacg tcaacaagca tcaggtgatt gtccgtgcca cccgaaacaa tcctccaccc
      841 ccgtcgtccc atggcatccg cgaggacctt tgcgttttta acaatttgcc cggaatactc
      901 cttaaaggct gggctcatgg cttccttaaa tgccaccgcc ttcgcggcaa tcacgtgcat
      961 cagaggcccg ccctggattc ccggaaacac cgaagaatcg atggccttgg catatttttc
     1021 cttgcacaaa ataagtcccg cccgcgggcc tcgcaatgtt ttatgggttg tcgtcgtcac
     1081 aatgtcggca tatggaactg gactgggatg atggcccgtc gccaccaaac ccgcaaaatg
     1141 agccatatcc accatcaaga gagcccccac gctgtccgct atttttttcc ctctctcaaa
     1201 atcaatcact cgagggtacg ccgaggcccc caagagaatc aacttgggcc ggttggcatg
     1261 agcaagacgt tcgagttcat cgtagtcaat ttgttcattg gccggactga caccataagg
     1321 aatgatggta taaaatttcc cggagtaatt catgggatga ccatgtgaaa gatgcccccc
     1381 gtgcgttaaa ttcataccta aaattttatc gcccggctcc agaagcgcca tgaacaccgc
     1441 gatattggcg gaggtgccgc tgtgaggttg aacattcgcg tgctccgcgc caaaaagctg
     1501 tttggcgcga tcgatcgcga gtcgctcgac cacatccacg ttttcacacc ccccgtacca
     1561 tcgctttccg gggtatcctt ccgcgtactt gttagtcaag accgagccca tcgtctcgag
     1621 aaccgatatc gacgtaaagt tctcgctcgc gatcaactca atgttctcgt tttgccgccc
     1681 gacttccgct cgtatcgccc ggtagatctc agggtccgaa gctttcagat gcgagtaatc
     1741 agtgacgtcc ttgttcataa gttttaattt ttttaacacg gatttcatga cgccccctat
     1801 cgaatggtgt attcagccaa gaatcaacta aaggaaaaag tatgtcttgg ggaagaaaac
     1861 ccgccccgag aatcagcata ttagagtcgt tgtgggccct ggagagccgc gcctgttcaa
     1921 tgttctgcgc caacgccgcg cggaccccgc aaactttatt cgcggcaatg gcacttccga
     1981 ttccggtgta acatatccca atggcacgat ggcattcttt tttagccact ttctcagcgg
     2041 cctgaaatat aaaatccgga taatcgcagg attccccggt atccgtcccg cagttaacaa
     2101 cttcaaaatt tttcttccgg agaaaatcga tcaacgcatt tttcatctga agaccgcgat
     2161 gatcgcaagc gatcgcaatt ctaggcttta tcataaaaat gatcctcgtg atagaagtta
     2221 aacaattttg tgaaaaatat tcctgatctt tttttcaatc tgattgatcg tcttttcgta
     2281 aaccatcatc cccaaaccaa tcggatcttc aacgtccagg cattggattt tttcccgcga
     2341 acccggcatt aattcttcga cagaatccgc gtgatgttcg gccatcacga gaatgacatc
     2401 ggctccccaa atatccgagt cccggcattg acggctttta aaacaactaa ggtcaacacc
     2461 ccggttcctc atcacatact ccgcttcact ggcggccgga agaccatccc tcgcgccaat
     2521 cccgcaggaa gccacttcaa tctcatccga aaggcccttc agctcgagct ccttcttcag
     2581 ccatccctcc gccatcggtg accgacatga attccccgtg cagaccacca gaattttctt
     2641 tttaggatat tgatcggttt taacacgggc gacggctttt tccacttccg tcccgtaggc
     2701 ctttttacgg aggatcaccg gttcttcacc gctcacatcc aacaccgtcg aatcatgact
     2761 aaattcagtc cgccccgtat caattaaaag cctgattttg cttcccaatt gctctaccac
     2821 ctggtgcgcc gtgtgaggcg acaacaatcc gctctgattg gcactg
//
LOCUS       DAOSIO010000195         2844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_30623_length_2844_cov_4.365005, whole genome shotgun sequence.
ACCESSION   DAOSIO010000195 DAOSIO010000000
VERSION     DAOSIO010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30623_length_2844_cov_4.365005"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="PK590_06975"
     CDS             complement(<1..886)
                     /locus_tag="PK590_06975"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOG24375.1"
                     /translation="MTRSDFVHLHCHTEFSLLDATCKIQGLMRRATELRFPALAMTDN
                     GNLFAAVQFYSQAMQAGIKPIIGMRAYVAPGSRLEKQAHGIKEASFHLTLLARNEEGF
                     RNLTRLSSIGYTEGFYYRPRIDKGVLKELGGDLIALSGGLKGEIPYYLYHDQIDEAKK
                     VIDEYISIFGKENFFLEVMDHGLDPEKKVIEESRKLAREFKIGLVATNDVHYIHRHEA
                     MAHDALICIGTGAKLDEPHRMRYQGDQYYLKSAEEMKEIFKEIPEAIENTVLIAKRCN
                     IEFDFKKLYLPKFKAPEGK"
     gene            complement(989..2530)
                     /gene="guaA"
                     /locus_tag="PK590_06980"
     CDS             complement(989..2530)
                     /gene="guaA"
                     /locus_tag="PK590_06980"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOG24376.1"
                     /translation="MNQTVVVLDFGSQYTQLIARRVRENRVFSKVYPFYTPFEKVMED
                     RPKGIIFSGSPANVYSKNAPVPDKRFFCAGIPILGICYGMQLLSSKLGGRVHKSKKRE
                     YGFSKLHISKPGNPLFEGIPKTITCWMSHADKLDKIPPGFERLAYTENAPFAAMGDSK
                     KKIYAIQFHAEVAHTELGSKILENYLVRVCGCKKSWTPRSFINESVKKIRQQVGRDRV
                     VLGLSGGVDSSVVAGLIHKAVGKQLTCIFVNTGLLRRNEVQRVDKTFRGHFKMNLKVI
                     DAEKIFLRRLKGVTDPEQKRKIIGKTFIDVFQKEANKLGKVPFLAQGTLYPDVIESVS
                     YFGGPTSMIKSHHNVGGLPKNMKFKLVEPLKELFKDEVRAVGYALGIDKEIVGRQPFP
                     GPGLAVRILGEVRKDRCDLLRLADWIVVDEIKKSGYYDKVWQSFAVLLPVRSVGVMGD
                     ERTYENAVAVRAVTSLDGMTADWAKLPYDLLGRISNRIINEVRGINRVTYDISSKPPA
                     TIEWE"
     gene            complement(2607..>2844)
                     /locus_tag="PK590_06985"
     CDS             complement(2607..>2844)
                     /locus_tag="PK590_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HOG24377.1"
                     /translation="PRGTRIHVGTTGSLEEILYGPARLDDGTQNLVGALRTSMGNVGA
                     RNIREMQLTEIVIAPAIKTEGKLFQAAQKLGMCK"
BASE COUNT          680 a          789 c          669 g          706 t
ORIGIN      
        1 tcttcccctc cggcgccttg aacttgggca agtagagttt tttgaaatca aactcgatgt
       61 tacaccgctt cgcgatgaga acggtatttt cgatcgcctc gggaatttcc ttgaagatct
      121 ctttcatctc ctcagcggat ttcagataat actgatcccc ctgatagcgc atccggtgcg
      181 gttcatccag tttcgcgccc gttccgatac aaatcaacgc gtcatgcgcc atcgcctcat
      241 gtcggtggat gtaatggacg tcattggtgg ccaccagacc gattttgaat tcgcgtgcca
      301 gcttgcggga ctcttcgatc actttttttt cgggatccag tccatggtcc atcacttcaa
      361 gaaagaaatt ctcttttcca aaaatcgaaa tgtattcatc gatcactttt ttggcttcat
      421 cgatctggtc gtgatagaga tagtaaggga tctccccctt caaccctcca gaaagagcga
      481 tcaggtcccc gcccagttcc ttcagaacgc ccttgtcgat ccggggccgg tagtagaaac
      541 cttccgtgta accaatgctg gaaaggcggg tgagatttct aaatccttct tcgtttcgcg
      601 cgaggagtgt gagatggaag gaggcttcct tgattccatg agcctgtttt tcgagccggg
      661 atccgggcgc cacgtaagcg cgcattccga tgatcggctt aattccggcc tgcatggcct
      721 gggaatagaa ctgaacggcg gcgaagaggt tcccattatc cgtcatcgcg agcgcgggaa
      781 accggagctc ggtagcacga cgcatcaggc cctgaatctt gcacgtcgcg tccagcaggc
      841 taaactcagt gtggcaatga agatgtacaa aatcgctacg agtcatgtct caggtgtctc
      901 tcggaaaaca actaccgggg aacaaaaacc aatctcgcta ttcttttcta aacgctcacc
      961 tttcaacgtt tcccgtttcc ctgcttaact attcccactc aatcgtggcg ggaggcttgg
     1021 aagaaatatc atacgtgaca cggttgatac cgcgaacttc attgatgatc cggttcgaaa
     1081 tgcgtccgag taaatcgtac gggagttttg cccagtccgc ggtcatgccg tccagcgagg
     1141 tcaccgcgcg aaccgcgacg gcattctcgt aagttcgttc atctcccatc acgcccacgc
     1201 tccggacagg gagaagcacc gcgaaggact gccacacttt atcgtaataa cccgactttt
     1261 taatctcatc cacgacaatc cagtccgcca accgcagaag gtcacaacgg tctttgcgga
     1321 cttcgcccaa aatacgcacc gccaaacccg gcccggggaa aggctggcgg cccacaattt
     1381 ccttatcaat tccaagggca tatccgaccg cgcgaacctc atctttaaaa agctctttaa
     1441 gaggctctac cagtttaaac ttcatatttt tcggaagtcc gccgacgtta tggtggctct
     1501 tgatcatgct cgtgggacct ccgaaataag aaaccgactc aatcacgtcc ggataaagcg
     1561 tcccttgcgc gaggaaaggc accttgccaa gcttgttcgc ttctttctga aaaacatcga
     1621 taaaggtttt gccgataatt tttcgctttt gctcgggatc cgtcactccc ttgagacggc
     1681 gcagaaaaat tttttcagcg tcaatgacct tgagattcat cttgaaatga ccgcgaaacg
     1741 tcttgtcaac gcgttgaacc tcattccggc gaaggagacc cgtattgaca aaaatgcagg
     1801 tcaactgctt cccgaccgcc ttgtggatca gaccggctac gaccgaagaa tcgactcccc
     1861 ccgacaaccc gagcaccacc cgatccctcc cgacttgttg tcgaatcttt tttacgcttt
     1921 cattgatgaa agaacgcggc gtccaactct tcttgcaccc acagacgcga accagatagt
     1981 tttcaagaat tttggaacct aactccgtat gcgccacttc cgcgtggaat tgaatagcgt
     2041 agattttctt cttcgaatca cccatcgcgg caaacggcgc gttttccgtg taggcaaggc
     2101 gctcaaaccc cggaggaatc ttgtccagct tgtcagcgtg actcatccag caggtaatcg
     2161 tcttcgggat tccctcaaaa agaggattgc cgggtttgga aatatggagt tttgaaaacc
     2221 cgtattcccg ctttttagat ttatgaaccc ttcccccaag cttggatgaa agaagctgca
     2281 tcccgtaaca aattccgaga attggaattc ccgcgcaaaa aaaccgtttg tccgggaccg
     2341 gggcgttttt cgaataaacg ttggccggcg aacccgaaaa aatgattcct ttgggccgat
     2401 cttccatgac cttctcaaaa ggcgtgtaga aaggatagac ctttgaaaag accctgtttt
     2461 cgcgaacgcg tcgcgcgatc agttgggtgt actgcgatcc aaaatccaga acaacgacgg
     2521 tttgattcat ggtttttatg tcctgcgtat ggcgtacgga gcgcggtgag acaaaacacc
     2581 acgcgcgaaa cgccttgcgt tcggtgttat ttgcacatcc caagtttttg agccgcctgg
     2641 aaaagcttac cttccgtctt gatcgccgga gcaatcacaa tctcggtaag ttgcatctcg
     2701 cggatgttcc gcgccccgac atttcccatc gaagtccgga gcgctcccac aagattctga
     2761 gtcccatcat caagacgagc gggaccgtaa agaatttctt ccagggaacc agtggtgccg
     2821 acatgaattc gtgtccccct cggt
//
LOCUS       DAOSIO010000196         2831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_30815_length_2831_cov_3.173271, whole genome shotgun sequence.
ACCESSION   DAOSIO010000196 DAOSIO010000000
VERSION     DAOSIO010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30815_length_2831_cov_3.173271"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PK590_06990"
     CDS             complement(<1..675)
                     /locus_tag="PK590_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOG24378.1"
                     /translation="MTDKSKKRLYESRIIQEDITQLAKNLKSYHKHFENSTFLVTGAG
                     GFLGNYITLLLRHMNQHHLHNKKLKAILLDNFITGYEQQIISDDHIVFRRQNVIEPVK
                     LDEKVDYIIHAAGIASPVYYTKYPIETMDVGTIGTRNMLELAREKKVKSFLFTSSSEV
                     YGDPDSRHVPTREEYRGNVAVIGPRACYDESKRYGETMCSAFWNVYETPVKMVRPFNV
                     YGPGIRP"
     gene            807..2072
                     /locus_tag="PK590_06995"
     CDS             807..2072
                     /locus_tag="PK590_06995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HOG24379.1"
                     /translation="MRHIQQIIEYFLRKKWLGILLITLLVSGVGVSTIYRGAFSQKQR
                     TDLAVFFKAAEMVHEGRAEHIYGIETRRHWHYVYTPLLAILLAPAVGLPFVIPVTIHY
                     VMSLAGLGIIFFLSRHFTPDKKNAVWQIVLSALLCIPVFLDTLTRGQLGILMLFFQTA
                     IFFSYLKNHKILAGFLLALAVSLKTSPLAVLFVYFLFRKEWSLLLSAACGLVFFIFLY
                     PSVIIGFEENWELLTIWHGLMKEGSSINAYKNYLWSELFTPFAADNQSFYAVLTRFCW
                     RGESDFIANPSHGVRWISLSMGIISVLLLFMKHTRSTPKSGEDVSTEDKLVEFSLYPV
                     AMLLFSPVTQIHHYVSLYFMFLAAFFLWNRKKTWLPLAGVFCSASLFLLALVIEPLAY
                     LGFPMWGAFILWGILLFCPTPSAKSSGGA"
     gene            complement(2058..>2831)
                     /locus_tag="PK590_07000"
     CDS             complement(2058..>2831)
                     /locus_tag="PK590_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG24380.1"
                     /translation="ALSDGRLGITLSLGEQTSETYERWFKAGAHRYLLRIETSHPELY
                     QKIHPGNHSFGERLDCLRALKRIGYQTGTGVMIGLPGQTREDLVNDIFFFEREDIDMI
                     GMGPYIPHADAPLARQSDLLMPEAGVSMENRPSGLSGFRYDAEDQLRLGLVMTALTRL
                     HLKNVNMVAATALQAIDDEGRERGILAGANIIMPNITPVKYRAKYQLYAGKPYLDEDA
                     CQCSHGLDRRIRATGAEPGYNEWGDSPHFRFGKNDLRSA"
BASE COUNT          621 a          774 c          649 g          787 t
ORIGIN      
        1 cggacggatt cccggcccgt aaacgttaaa agggcgtacc attttcaccg gtgtctcgta
       61 aacgttccaa aaagccgagc acatggtttc accgtaccgt ttcgattcgt catagcaggc
      121 ccgaggcccg ataaccgcca cgttaccgcg atattcctcc cgggtcggga catgccgact
      181 gtccggatcc ccgtaaactt cactcgaact cgtaaaaaga aaacttttca cttttttttc
      241 gcgggcgagc tcaagcatat tccgggtccc tatcgttccc acgtccatgg tttcaatcgg
      301 atacttggtg taataaaccg gtgaggcaat ccccgcggcg tgaataatgt aatccacttt
      361 ttcatccaat ttcaccggct caatgacatt ctgccgacgg aaaacaatgt ggtcgtccga
      421 aatgatttgc tgttcatagc ccgtgatgaa attatcaaga aggattgcct taagcttttt
      481 gttgtggaga tggtgctggt tcatatgacg caagagaagc gtaatgtaat tcccgagaaa
      541 tccccccgcc ccggtcacaa ggaaagtact gttctcgaaa tgtttatgat aggacttcag
      601 gttttttgcc agctgtgtga tatcttcctg gataattctg gactcataaa gacgcttttt
      661 cgatttatcg gtcaccgcat ccacctctct cggatcatcg tcaccattta ttcgactggt
      721 taccgccggg gaaacccacg cagtttcttg cctcagaaaa gagttgtgat tatagcctaa
      781 atcgtgtaga agaacgaaat aaaattatgc ggcacatcca gcaaatcatc gaatattttc
      841 tccgaaagaa gtggctcgga attcttctca tcactctcct tgtgtcggga gtcggcgttt
      901 ccaccattta ccgcggagca ttcagccaga aacaaagaac ggatcttgcc gtttttttca
      961 aagcggcgga gatggtgcat gaaggacggg cagaacatat ctacgggatc gaaacccggc
     1021 ggcactggca ttatgtctat acgcccctac tcgcgatttt gctcgcgccc gcggtggggc
     1081 tcccgttcgt cattcccgtc acaatccatt acgtcatgtc gctggcgggt cttggaatca
     1141 ttttctttct ctcgcgacat tttacgcccg acaaaaagaa cgcggtctgg caaatcgttc
     1201 tttcagccct cctttgcatt cccgtttttc tggacactct gactcgtggg caactcggca
     1261 ttctcatgct tttttttcag acagcgatct ttttttctta tttgaaaaat cacaaaattt
     1321 tggccggatt tctgctggca ctcgcggtca gcctcaagac cagtccgctt gccgtccttt
     1381 ttgtttattt cctgttccgc aaggaatggt cccttctgct gagcgccgct tgcgggctcg
     1441 tttttttcat attcctttat ccgtccgtca taatcgggtt tgaagagaac tgggaactgc
     1501 tgactatctg gcacggtctc atgaaggaag gatcatcaat taacgcgtat aaaaattact
     1561 tatggtcaga actcttcacg cctttcgcgg ccgacaatca gtctttttac gcggtcctga
     1621 cccgcttttg ctggcgggga gaatcggatt ttatcgccaa tccttcccat ggggtgcggt
     1681 ggatctctct atcaatgggg atcatctccg tcctcctgct tttcatgaaa cacacgcgct
     1741 cgacgccaaa atccggagag gatgtatcga ccgaggacaa gttggtcgaa ttttcacttt
     1801 atcccgtagc catgctcctt ttttctcccg tcactcaaat ccatcattac gtttctctct
     1861 acttcatgtt ccttgccgct ttttttcttt ggaaccgaaa aaaaacatgg cttccgctcg
     1921 ccggagtctt ctgctccgcg tctttgtttc ttctggctct ggtgatcgaa cctcttgcct
     1981 acttgggatt tccaatgtgg ggtgctttta ttttgtgggg aattcttttg ttctgtccga
     2041 ctccctccgc gaagagttca ggcggagcgt aaatcgtttt ttccaaatct gaaatgcggt
     2101 gagtcgcccc attcgttata tccgggttca gctcccgtgg cgcgaatcct ccggtcaaga
     2161 ccgtgagaac actggcaggc atcttcatcc aggtaaggct tgcccgcgta aagctggtac
     2221 ttcgcgcgat atttcaccgg cgtgatattc ggcatgatga tattagcgcc ggccagaatt
     2281 ccccgttccc ggccttcgtc atcgatcgcc tgaagcgccg tcgccgctac catatttaca
     2341 tttttaagat gaagccgggt gagcgcggtc atcaccagtc caagccgcaa ctgatcttcc
     2401 gcgtcatacc tgaaaccact caatcccgaa ggtcggttct ccatgctgac gccagcctcc
     2461 ggcatgagca gatcggactg cctcgccagc ggggcatcgg catgcgggat ataaggcccc
     2521 atcccgatca tatcgatgtc ttccctttca aaaaagaaaa tatcgttcac aagatcctcg
     2581 cgggtctggc ccggcaaccc gatcatcacg ccggtacccg tctggtaacc aattcgcttc
     2641 agcgcgcgaa ggcaatcgag tctttcgcca aatgaatgat taccgggatg gattttctga
     2701 tacagttcgg ggtgactggt ttcgatcctc agaagatagc gatgagcgcc cgctttaaac
     2761 cacctttcgt aagtttcgga cgtctgctca ccaagagaaa gcgtaatccc gagccgtcca
     2821 tcactcaacg c
//
LOCUS       DAOSIO010000197         2785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_31468_length_2785_cov_3.947253, whole genome shotgun sequence.
ACCESSION   DAOSIO010000197 DAOSIO010000000
VERSION     DAOSIO010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31468_length_2785_cov_3.947253"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PK590_07005"
     CDS             complement(<1..818)
                     /locus_tag="PK590_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOG24381.1"
                     /translation="MISNDDRLSFEKSRLGRRSVTLPKGRLSEPLIASQVSRQSELAF
                     PEMSELDTVRHFINLSRKNFSVDTHFYPLGSCTMKYNPKVNEWVARQEKFTSLHPLQP
                     LETAQGMLRVLWELEDALKEITGMDRFTLQPAAGAHGELTGIMLVRAYHASRGDLSRK
                     KVIVPDSSHGTNPATAALVGYRVVEVASDARGRVDLNNLKKVLDDQVACLMLTNPNTL
                     GVFEEDIIEIARLVHKEGGLLYYDGANLNPLLGIARPGDMGFDIVHVNLHKTFST"
     gene            complement(815..2152)
                     /gene="gcvPA"
                     /locus_tag="PK590_07010"
     CDS             complement(815..2152)
                     /gene="gcvPA"
                     /locus_tag="PK590_07010"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF014408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOG24382.1"
                     /translation="MPYLPYTEQEKQRIFREIGVSSLDDLIQSIPSSLRHPQILMSHG
                     LSEIEVQRQVLKLGSRNTATTDFLSFLGAGSYEHFIPSAVQEMVARQEFTTAYTPYQP
                     EASQGTLQVIFEYQSLMAELTGLAASNASHYDGATSLAEAALVALRQTDRTKILVARS
                     LHPHYRDVIRTYLAQSSYQVEEFGFLAHGGLDLESLQKKLTPDVAGVILQTPNFFGGV
                     EDLNGVSDRVHANGSLLIVSGHPLSFALYRSPGEWGADIAAGEGQPLGMPLGFGGPYL
                     GYFVASQSLLRRIPGRIAGLTEDTDGKRAFCLTLQAREQHIRRERAGSNICSNQALCA
                     LAACVYMTFLGKQGMREVAELNVDRAHYLRERVAKIKGFKVDLERPIFNEFVLHCSRP
                     VAEICKKMMAARIFPGVRLGDYYPEFKDCLLVCATETKTGEDLDRFLEVLSQC"
     gene            complement(2166..2558)
                     /gene="gcvH"
                     /locus_tag="PK590_07015"
     CDS             complement(2166..2558)
                     /gene="gcvH"
                     /locus_tag="PK590_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOG24383.1"
                     /translation="MNFPENLRYTKTHEWISLTGHKARVGVTEYAQKEISDVVFVELA
                     KKGHPVEQGKAASVVESVKAAFDIYAPVSGTITDANGALEANPGLVNQDPYNDGWLFE
                     LEVSDPLSVDALMTSAQYAAFVQSGSAH"
BASE COUNT          741 a          769 c          651 g          624 t
ORIGIN      
        1 gtcgaaaagg ttttgtggag gttaacgtgg acaatatcaa atcccatatc acccgggcgc
       61 gcgatcccaa gaagtggatt taaattcgcg ccatcgtaat agagtagccc gccctcctta
      121 tgcacaagcc tggcaatctc aataatatct tcctcaaaaa cacccagcgt attcggattg
      181 gtcagcatga gacaagctac ctgatcatct aaaacttttt tgaggttatt gagatccact
      241 cgcccgcgcg catcagaagc gacctcaaca acgcggtagc ccaccaaagc ggccgtcgcg
      301 ggattagtgc cgtggctaga atccgggaca atcacctttt tacgacttaa gtctccacgg
      361 gaagcgtgat aagctcgaac cagcataatt cccgtcagtt ctccgtgcgc gcccgccgcg
      421 ggctgaagcg taaaccggtc catacccgta atctctttca gagcatcttc cagctcccac
      481 aaaacccgca acatcccctg ggccgtctcc agaggctgaa gcggatggag gcttgtgaat
      541 ttttcctgac gcgcgaccca ttcattgacc ttggggttgt acttcatcgt acaacttccg
      601 agagggtaaa aatgcgtatc caccgaaaag tttttccggg acaaattaat aaagtggcgg
      661 acggtatcaa gctcgctcat ttccgggaaa gccaactcac tctgacgaga cacctgggaa
      721 gcgatcaggg gttcagagag ccgccctttt ggaagcgtca cacttctgcg gcctaaccgg
      781 cttttctcaa aactcaatcg atcatcattg gaaatcaaca ttgggaaagc acctccaaaa
      841 accggtcaag atcttcaccc gtcttcgttt ccgtcgcgca aaccagaaga cagtccttaa
      901 actcgggata ataatcaccc agtctgacgc cggggaaaat acgagccgcc atcatttttt
      961 tgcaaatttc agccactggg cgcgaacaat ggagcacaaa ctcattaaag atgggtcttt
     1021 cgagatccac cttaaaccct ttgatttttg ccactcgctc acgaagataa tgagccctgt
     1081 caacattgag ctcggcaacc tcccgcattc cctgtttccc aagaaacgtc atgtaaacgc
     1141 atgccgcgag cgcgcataaa gcctgattgg agcaaatatt ggatcccgct cgctctcttc
     1201 gaatatgctg ttcccgggcc tggagcgtaa gacaaaacgc ccgcttcccg tcagtgtcct
     1261 cagtcaaccc ggcgatccgc cccgggattc gccgaagcaa cgattgagac gcgacaaaat
     1321 acccgagata aggccctcca aaaccgagcg gcattcccaa aggttgtccc tcaccggccg
     1381 caatgtcagc gccccattct cccggagatc gatacagcgc gaaagaaagc ggatgcccgg
     1441 agacgatcag gagcgatcca ttggcatgaa cacggtcgga aacgccgttt aaatcttcga
     1501 cgccaccgaa gaaattcggc gtctgcaaaa taacgcccgc gacatcgggt gtcagttttt
     1561 tctggagaga ctcaagatcc aatccaccat gagccagaaa accaaattcc tcgacttggt
     1621 acgagctttg ggcgagataa gtccggatca catcgcgata gtgcggatgg agcgatcgcg
     1681 cgacaaggat cttcgttcga tccgtttgtc tcaacgccac aagcgccgct tcggccaggc
     1741 tggtcgcccc gtcataatgg gacgcgttcg aggccgcaag accggtaagt tccgccatca
     1801 aactctggta ttcaaaaata acctggagag tcccttggga cgcttcgggc tgatacggag
     1861 tataagccgt ggtaaattcc tgtcgggcca ccatttcctg gaccgctgaa ggaataaaat
     1921 gttcgtaact tccggcgcca agaaaactga gaaaatcggt cgtcgcggtg ttgcggcttc
     1981 ccagtttcaa aacctgccgt tggacctcaa tctcactcaa tccgtgagac atcaaaattt
     2041 gaggatgacg gagagaagaa gggattgact gaatcagatc atcaagcgat gagactccaa
     2101 tctcgcgaaa gatcctctgt ttctcttgtt cagtataggg taggtaaggc attgctgaca
     2161 gacaatcagt gagcggatcc ggattgtaca aatgccgcgt actgggctga agtcatcaaa
     2221 gcgtcgaccg acaaggggtc agatacctcg agctcaaaaa gccagccatc attatacgga
     2281 tcctgattca caagtcccgg atttgcctca agcgctccat tggcatccgt aatcgtacct
     2341 gaaaccggtg cgtaaatgtc aaaagccgcc ttgaccgact ccacgacaga cgcggctttc
     2401 ccctgctcaa ccggatgccc ttttttagcc aactcgacaa acacaacatc agaaatttct
     2461 ttttgggcgt attcggtaac gccaacccgc gccttatgac ccgtgaggga aatccattca
     2521 tgtgttttgg tataacgaag attttcagga aaattcattt taacctccat cgactttata
     2581 atgacgcgag gacagacaaa tttttgaaat ataaaagcgg cctgtcccct ttggaagggc
     2641 cgtattctac tattttcgct tgtaaaaagg tagggaaact acgcgtgctt tcaaagaatg
     2701 accacgaacg acaatatcga tttcattatt ttcccgcgcc tcggcagaag aaacataccc
     2761 catcccgata ttccggcctg gagtt
//
LOCUS       DAOSIO010000198         2747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_32026_length_2747_cov_2.085067, whole genome shotgun sequence.
ACCESSION   DAOSIO010000198 DAOSIO010000000
VERSION     DAOSIO010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32026_length_2747_cov_2.085067"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PK590_07020"
     CDS             complement(<1..926)
                     /locus_tag="PK590_07020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012236.2"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOG24384.1"
                     /translation="MEELRYKETGIIKAVSGCIVRVTGLQNCFLGQMIQFGYGTQGVI
                     MGFDLTEAQVLMVKENEPLVPGNPAIATLEPFNMPVGPGAMGRIINPLGEAMDGLGPI
                     EFDGFIPYFNEAPAIMDRAPLSRSFETGIKLIDSMLPLGKGQRSLILGDKMTGKTTIG
                     TDIILNQKGKDVLCIYCGIGKTASAMGKVVSLFKEHDCFEYTTILCAPASSSPGQQYL
                     APYAACSIGEYFRDRGKDVFVFFDDFTKHAWAYRELSLLMGVPPGRDSYPGDIFYLHS
                     RMIERAAQLDKQHGGGSMTFIPVVELLEGDLTA"
     gene            complement(936..2207)
                     /locus_tag="PK590_07025"
     CDS             complement(936..2207)
                     /locus_tag="PK590_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24385.1"
                     /translation="MNPSVDQSLKAVIDQPLPAEIDPAQDIVDKAGLEIVYDAEAKGY
                     KLTGGVELKPRETKTFEVRIRDVWQISVDQAEEVRKELDAQITALKGTKYYETAKLLY
                     DKAQEGIDRILEEQSKPMGISQHIELYRSHVLQLQDIKSNAISLSVMRKTEEEQKKGI
                     PEARFVIDAENPSSESRSMRVRSVLPKELKAEDVLDRQGFSVLYDQLRKAYVLEKDDQ
                     FSAREKKTYVITVRDIWRVSDEEIGYHREQTEQLVAFFEGTVFEKYAAGQGAVILDIL
                     DGITQLQAELNSSLALEDRMRAFVLNAQQMNIAKEKIRNLQHLMVEVSPKKNDHELLE
                     KIKHFVKKLAEVKDVVLMAMGVRPDTPIVWWIIFGIIIFLGIVSVVFYMIWIKRLREE
                     KWGPKEKGKGASPAVSQEPPVSDESKTEQGS"
     gene            complement(2464..>2747)
                     /locus_tag="PK590_07030"
     CDS             complement(2464..>2747)
                     /locus_tag="PK590_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24386.1"
                     /translation="SDTNQKAETLVNKLVALANQQAKELGFIGEEIKALSASESSNIA
                     LIQSKLEEIKTYLLAVKEATDASARGALSQDQEVPSAVVKSWLEMGEGA"
BASE COUNT          718 a          744 c          571 g          714 t
ORIGIN      
        1 gccgtcagat cgccttcgag cagttcgacc accgggataa aagtcataga acctccgcca
       61 tgctgtttat caagctgagc ggcccgctca atcatgcgcg aatgcaaata gaaaatatct
      121 cctggatacg aatcgcgccc cggaggaacc cccattaaaa gggaaagttc acggtatgcc
      181 cacgcatgtt tggtgaaatc atcaaaaaac acaaaaacat ctttcccgcg atctctgaaa
      241 tattcgccaa tggaacaagc tgcgtaaggc gccaaatact gttggcccgg cgaactcgaa
      301 gccggagcgc acagaatcgt tgtatactca aaacaatcat gctctttaaa aagcgaaacg
      361 acttttccca tcgcagaggc tgtcttgcca atcccgcagt aaatgcagag aacatcttta
      421 cccttctggt tcaaaatgat gtccgttccg atcgtagttt tgccagtcat cttatcgccg
      481 agaattaatg atcgctggcc cttccccaac gggagcatcg aatcaattag tttgatccct
      541 gtctcaaacg agcgactgag aggggcacga tccataatgg cgggggcctc attaaaataa
      601 ggaatgaaac cgtcaaattc gatcgggcct agtccatcca ttgcctctcc aagagggttg
      661 ataatacgtc ccatggctcc ggggcccacc ggcatattaa agggttccag ggttgcaatg
      721 gcgggattcc cgggcaccag aggttcgttc tctttgacca tgagtacttg tgcctccgtc
      781 aaatcaaacc ccatgatgac accttgagta ccgtatccaa attgaatcat ctggcccaaa
      841 aaacagttct gaagccctgt gacccggaca atgcaaccgc taacggcctt gataataccc
      901 gtttccttgt aacgcagttc ttccatagtc ctggattaac ttccttgttc tgttttggac
      961 tcatcggaaa cagggggttc ctgggatacc gccggagaag cacctttccc cttttccttg
     1021 ggaccccatt tctcctcccg gagccgcttg atccaaatca tgtaaaaaac aacactgacg
     1081 atgcctagaa aaataattat tccaaaaata atccaccaaa caatgggtgt atcaggacgc
     1141 acccccatcg ccataagaac aacatccttg acttccgcga gttttttgac aaagtgtttg
     1201 attttttcaa gaagttcatg atcgtttttc ttgggagata cctccaccat taagtgctga
     1261 agattccgga ttttctcctt tgcgatattc atctgttggg cgttcaaaac aaaagcccgc
     1321 atccgatcct ccagtgcaag cgatgaattt aattccgctt gcaattgcgt gatcccatcc
     1381 aggatatcca gaatcaccgc tccctggccc gccgcgtatt tctcaaacac cgttccctca
     1441 aaaaacgcca ccaactgttc agtctgctcc ctgtgatacc cgatttcctc atccgacaca
     1501 cgccagatat cccggaccgt gatgacgtag gttttctttt cccgcgcaga aaactggtca
     1561 tctttctcaa gaacatacgc tttccgcagt tgatcataca aaacggaaaa accttgtcga
     1621 tccagcacat cctcggcttt tagctctttc ggcaagacag aacgaactct catcgatctt
     1681 gactcactag agggattttc cgcgtcaata acaaagcgtg cttccggaat tccttttttt
     1741 tgttcttcct ccgtcttccg catgacgctc agggaaatag cgttggactt aatatcctgg
     1801 agttgaagaa cgtgagagcg gtaaagttca atatgctggc tgatccccat tggcttgctc
     1861 tgttcttcca gaattcgatc aatcccctcc tgggccttgt cgtaaagcaa ctttgccgtc
     1921 tcgtaatact ttgttccctt cagcgcggta atttgagcat ccagttcctt tcgaacttct
     1981 tcagcttggt cgacagaaat ctgccaaaca tcacggattc ggacctcaaa ggtcttggtt
     2041 tctcggggct taagctccac tccacccgtc agcttgtacc ccttggcttc agcgtcataa
     2101 acaatttcaa gacctgcctt gtcaacaata tcctgagcag ggtcaatctc ggctggcaaa
     2161 ggctgatcga ttacggcctt cagagattgg tcaacggagg gattcaccgc aaggatctta
     2221 aacttgactt caaaatcagc attgattgag ccgaaaaagg aaaacgtaca aaacacccat
     2281 ccaacaatga gtgagacaac ccttggtgag ttcatggatc agaaacttat gtcggtcttc
     2341 aagttgtaag tattcattcg gccaatgact ttctcgtaaa tgaagctact agaatcagac
     2401 tgacaagtat ccccccccag tattttccaa gggaggaaga aaaataaact tacaaaacca
     2461 tgcctaggcg ccttcaccca tctcaagcca tgacttaaca acagccgagg gaacctcttg
     2521 gtcttggctg agcgcccccc tcgcgctggc atcagtggcc tctttaactg ccaaaagata
     2581 ggttttaatc tcttcaagtt tcgactgaat taaagctatg ttggaggatt cgcttgcgct
     2641 aagcgctttg atttcttctc cgataaaccc aagctccttc gcctgctggt tggccagggc
     2701 gacaagttta ttcacaagcg tttccgcctt ttggttggta tcactgc
//
LOCUS       DAOSIO010000199         2746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_32031_length_2746_cov_4.185061, whole genome shotgun sequence.
ACCESSION   DAOSIO010000199 DAOSIO010000000
VERSION     DAOSIO010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32031_length_2746_cov_4.185061"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="PK590_07035"
     CDS             complement(<1..779)
                     /locus_tag="PK590_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOG24387.1"
                     /translation="MKGQVKETPAWLLYAVLGTLLLAAFLGSVSVGSLKFSWISALRH
                     PSSEMSFVFWDIRVPRAVLGLLVGATLGLAGAAMQGFLRNPLAEPGILGVTGGAALGA
                     VTVFYTGLVTLFPMVLPAGGILGAFISVILLYLFAGTLASVQTLILAGVAINVIAFAG
                     TSLMLNLVRDPYAVMDIVFWQMGSLADRSFQHVLLIFPFVMAGWALLLWDGRALDALS
                     LGEETASSLGISLKALRARIILGTALCVGASISVCGSIGFVG"
     gene            complement(776..1801)
                     /locus_tag="PK590_07040"
     CDS             complement(776..1801)
                     /locus_tag="PK590_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG24388.1"
                     /translation="MKQWPQSVTADARRTGSGVFHRSWCVARWFLFGLVATCFFIFLT
                     AESVLASEKAESSVPEEKSQDSFSEKNSSPRRIVSINLCTDELLLRLVEPGRVAALTR
                     FSDNADVSTVWETAQGIKKTELDIESILACSPDLLLGGRFNNREALHFFQYTDIPVII
                     FGVPKSFEDIYRDIRTLAEAVGEPEEGETIIRQMQTGLAELKAEAGESRKKILLSGAT
                     QDDGKGNKRRAIFFQSGGFVPGSETFENAIMEAAGLENLAATLGIKDYGSLSLEKLIE
                     MKPDILIFASDQKKQPTVRGEVLAHPAIRKSLSHVKTVTLPSATLSCGSPASVEAVRI
                     LVKETAL"
     gene            complement(1830..2324)
                     /locus_tag="PK590_07045"
     CDS             complement(1830..2324)
                     /locus_tag="PK590_07045"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOG24389.1"
                     /translation="MKISGWMILVFCLCPAANLHAQEHAGQGWGVTDPRQRSVGGIVS
                     SVTYGDVRFHGDAAGQVSGLDAREKESMIPFPILVPKAILYPRQAVRKKWEGQAIVAA
                     EVLPDGSVGRMGLSKSSGYEILDEAAKSSIKAWKFAYPFESQGALFSQYVDIPVTFRL
                     EESD"
     gene            complement(2364..>2746)
                     /locus_tag="PK590_07050"
     CDS             complement(2364..>2746)
                     /locus_tag="PK590_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG24390.1"
                     /translation="NLTTQKPYFKGGDYANKEIPFVPNFKMATGFVLEPVEGLTLSAE
                     LNHLGSRFAISDQSNDQAKMKSYTTVDMKARYQWKWATAWVALTNIFDSKFSEYAAYS
                     TTRGEVGYYPAQGRSLTTGFSLEY"
BASE COUNT          727 a          836 c          612 g          571 t
ORIGIN      
        1 ccgacaaaac caatgcttcc acacacagaa attgaagccc caacgcaaag cgccgtcccc
       61 aaaataattc tcgcccgaag ggcctttaat gatatgccaa ggctggacgc cgtttcttcc
      121 cctaggctta acgcgtccag cgcgcgtccg tcccaaagaa gcaaggccca tcccgccatc
      181 acaaagggaa agatcaggag tacgtgctga aaactgcggt ccgcgagcga ccccatttgc
      241 caaaaaacaa tatccattac cgcgtaaggg tctctgacaa gattcagcat gagggaggtt
      301 cccgcaaacg cgatcacatt aatcgccaca cccgccagga taagcgtttg aacgctcgcc
      361 aacgtccccg cgaaaagata aagaagaatc acagaaataa acgcccccag gattcccccg
      421 gcggggagaa ccatcggaaa caaggtgaca agcccggtat aaaaaaccgt gacggccccc
      481 agcgccgcgc caccggttac tcccaaaatt cccggctctg ccagcggatt acggagaaat
      541 ccctgcatcg cggcgcccgc caacccgagc gtcgcgccaa ccaggagccc gagcaccgct
      601 cgcggcacgc gaatatccca gaaaacaaac gacatctcgg aagaaggatg acggagagcg
      661 gagatccagg aaaacttcag ggaaccgacg ctaacggagc cgagaaacgc cgcgagaagt
      721 agcgtcccca ggacggcata aagcaaccag gccggcgttt ccttgacctg tcctttcaca
      781 acgctgtctc cttcacaaga atacgcaccg cttccaccga cgccggagaa ccgcaactca
      841 aggtcgcgga cggaagggtc acggttttta catgcgacaa cgacttacgg atcgcgggat
      901 gcgccaggac ttctccacga accgtgggtt gttttttctg atcgcttgcg aaaatcaaaa
      961 tatccggttt catttcgatc aacttttcca aagaaaggct tccgtagtcc ttgatcccaa
     1021 gggtggccgc gagattttca agacccgcgg cttccatgat cgcgttctcg aacgtctccg
     1081 aacccggcac aaatcctccg ctctgaaaaa agatcgcgcg acgcttattc cctttaccgt
     1141 catcctgggt tgccccggag agaaggatct tctttctgga ttctccggcc tcggctttca
     1201 gttcggccag ccccgtctgc atttgccgaa tgatcgtttc accttcttcc ggctcgccga
     1261 ccgcttccgc gagtgtacgg atatcccggt aaatgtcctc gaaactttta ggaacaccaa
     1321 aaataatgac gggaatatct gtatattgaa aaaaatgcaa cgcttcccga ttattaaacc
     1381 ttccgccaag gagtaagtcg gggctacagg ccagaatgct ctcaatatcc agttcggttt
     1441 tcttgatccc ttgcgccgtt tcccagacgg tcgacacatc agcattgtca ctaaatctcg
     1501 tcaacgccgc gacccgtccg ggctccacaa gtcttaaaag caactcatcc gtgcaaagat
     1561 tgatagagac aatacgccgc gggctggaat ttttttccga aaatgaatcc tgactttttt
     1621 cttcggggac agagctttcc gccttttccg aggccaacac ggattcagct gtgagaaaaa
     1681 taaaaaagca cgtcgcgacc agcccaaaca gaaaccaacg cgcgacacac cacgaacggt
     1741 gaaaaactcc agatcccgtc cgtcgcgcgt cggccgttac actttgcggc cattgcttca
     1801 tttcagcacc ttactaaaaa agcaacttct caatccgatt cttccaatct gaacgtgact
     1861 ggaatgtcaa catactggga gaacaacgcg ccctgcgatt caaacggata cgcgaacttc
     1921 caggccttaa ttgagctttt ggccgcttca tccagaatct catagccaga agatttcgaa
     1981 agacccattc gccccaccga accgtccgga agcacctccg cggcaacaat cgcctgccct
     2041 tcccattttt ttcgaacagc ctgccgggga taaagaattg ctttgggcac cagaataggg
     2101 aacggaatca tagactcctt ctcgcgcgcg tccagtcctg acacctgtcc tgccgcgtct
     2161 ccatgaaaac ggacatcacc gtatgtcaca gaagatacga tcccgcccac agaacgctgt
     2221 cggggatccg tgacacccca tccctgcccc gcatgttcct gtgcgtgcag attcgcggcg
     2281 ggacaaaggc aaaacaccaa aatcatccaa ccacttattt tcatcaaaat atctacctcc
     2341 ttcaaatcca tctacccgtc agcctaatat tccagcgaaa aacccgtggt caaacttcgc
     2401 ccctgggcag ggtaatatcc tacctcgccg cgagtagtac tgtaggcggc atattccgag
     2461 aatttcgagt caaaaatatt tgtcagcgcg acccaagcag tggcccactt ccactgatat
     2521 cgcgccttca tatctaccgt cgtgtacgat ttcattttcg cctgatcatt actctgatcg
     2581 ctaatggcaa accgggaccc gagatgattt aattcggccg aaagtgtcaa gccttcgaca
     2641 ggctccagaa caaaacccgt ggccatttta aaattcggca caaacggaat ctctttattg
     2701 gcataatccc cgcccttgaa ataaggtttc tgggttgtca gattca
//
LOCUS       DAOSIO010000200         2709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_32624_length_2709_cov_4.639035, whole genome shotgun sequence.
ACCESSION   DAOSIO010000200 DAOSIO010000000
VERSION     DAOSIO010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32624_length_2709_cov_4.639035"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..494)
                     /locus_tag="PK590_07055"
     CDS             complement(168..494)
                     /locus_tag="PK590_07055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HOG24391.1"
                     /translation="MSNKARIHRILGAFLIATFLTGGLSLAVATKAYGAPKAESQVNA
                     PVMININKAGVEELDKIRGIGPAMAQRIVDFRTQNGAFKSVDDLVQVRGIGGAKLQQI
                     KAQVTV"
     gene            640..1194
                     /locus_tag="PK590_07060"
     CDS             640..1194
                     /locus_tag="PK590_07060"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOG24392.1"
                     /translation="MLKNLGQRIRTLRKAKGLTLVEISEKTGVAQATLSRIETGTMVG
                     TVECHEKIAETLGIGLAELYSGVDKRYEQIARQDKDSRKVTHHTKDVHIELLTQESSS
                     KKITPLLLTLQSGGKTEKESNERGVEKFLWVLDGSVKIRLDQEEIVLKSQESIYFDAS
                     LPHQIFHESGKTAKVFIAVSPSKI"
     gene            complement(1300..1617)
                     /gene="cutA"
                     /locus_tag="PK590_07065"
     CDS             complement(1300..1617)
                     /gene="cutA"
                     /locus_tag="PK590_07065"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOG24393.1"
                     /translation="MKYCCIVSAVPNIKEARRLAGLLLASKIAACVQILPGMESHYCW
                     KGKRERSREVLLLIKTRASLYAKVEKTILKYHSYEVPEIICFPIEKGSPSFLKWVAEN
                     ARV"
     gene            complement(1623..1826)
                     /locus_tag="PK590_07070"
     CDS             complement(1623..1826)
                     /locus_tag="PK590_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOG24394.1"
                     /translation="MIMEIGIVAGEIWHYLDEHHTANLDQLTKGLKRPRELVLMSLGW
                     LAREGHVSLGTPESGYAATLNRK"
     gene            complement(1929..>2709)
                     /locus_tag="PK590_07075"
     CDS             complement(1929..>2709)
                     /locus_tag="PK590_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG24395.1"
                     /translation="IEIDQSPIGRTPRSNPATYTDIFGFIRRLFSSLETSKLRRYTQS
                     RFSFNLKGGRCEKCRGVGFEKLEMSFMPDLFVVCEDCRGQRYNASTLEVRYQGKNIAE
                     VLDLSILEAKDFFSKFSYVREKLEVLDSIGLGYVKLGQSSTTLSGGEAQRIKIASELM
                     KKSTGKTLYLLDEPTTGLHFSDIKNLLKALFDLRQQGNTVVVIEHNLDVIKMADYLID
                     LGPEGGQAGGNIVVCGSPEEVAGAPLSHTGRFLGEALARRP"
BASE COUNT          718 a          724 c          626 g          641 t
ORIGIN      
        1 gaacagttat tttagaggac ggggagtttc cccaaccttt cagttatttg aaaaatctct
       61 ggttcccatt aaagttttca gagagcgctt cccgtcctcc ccgcgacctg ccggccggac
      121 aggtccagag agcaacgaga aacgatcctt cttacgggtc aaatacgtca gaccgtaacc
      181 tgggccttaa tttgctggag tttggcaccg ccaatcccgc gaacctggac cagatcatcc
      241 acgcttttaa acgctccatt ctgcgtgcga aaatctacaa ttcgctgggc catggcaggt
      301 ccaattcctc gaatcttgtc cagttcctcg acgccggcct tgttaatatt gatcatcacc
      361 ggcgcattaa cctgagactc agctttaggc gccccgtacg ctttcgtggc aaccgccagg
      421 ctgagacccc cggtcaggaa cgtcgcgatc aaaaacgctc ccaaaatgcg atggatcctt
      481 gctttgtttg acattgaaac acctcctgtt ttgatttgag gttaccttaa cataagtatt
      541 tatatttgtc aatattaata tgagttttat ttatatttaa attatgcact tctttcatat
      601 ttcccttgac gttacagaag gaatggctag actggtttca tgctgaaaaa cttgggacaa
      661 agaatcagaa cgctccgaaa ggccaaggga cttacgctcg tcgagatttc cgaaaagacg
      721 ggcgtcgcgc aggcaaccct ctcacgcatt gaaaccggca ccatggtcgg aacggttgaa
      781 tgtcacgaga aaatcgccga aacactcggg atcggtttgg cggaacttta ttccggcgtc
      841 gacaaacgct atgagcaaat cgcccgccag gacaaagatt cccgcaaagt cacccatcac
      901 accaaagacg tgcacatcga attacttacc caggaaagct ccagcaagaa aatcacccct
      961 ctgctattga ccctccagag tggcggcaag acggaaaagg aatccaacga acggggcgtg
     1021 gaaaagttct tgtgggtgct ggacgggagc gtcaaaatca ggctggatca ggaagaaatc
     1081 gtactgaagt cgcaggaatc tatctacttt gacgcgtccc tgcctcatca aatctttcat
     1141 gaaagcggaa agaccgcgaa agttttcatc gccgtatcac cttccaaaat ctaggctccg
     1201 cgcctctcac aaaatccagc ctcgtgaaaa acatcgggaa tttgaagcgg gcgtctttca
     1261 agcgattgaa aaatcaagga tggactcaga aaagcgtccc tagactctcg cgttttcggc
     1321 aacccactta agaaaggacg gactaccctt ttcgatcggg aaacagataa tttcaggaac
     1381 ttcgtaagag tggtacttga gaatagtttt ttcgactttg gcgtagagag acgctcttgt
     1441 tttgattaaa agaagaactt ccctggatct ttccctcttc cccttccagc aataatggga
     1501 ttccataccg gggagaatct gaacacatgc ggcaattttc gaggcaagca aaaggcccgc
     1561 cagacggcgg gcctctttaa tgttcggaac agcggaaacg atacagcagt atttcacttg
     1621 ggttatttgc gattgagagt cgcggcgtaa ccgctctccg gggttcccag tgaaacgtga
     1681 ccttcccgag caagccatcc aaggctcatc aaaacaagtt ccctgggacg cttgagacct
     1741 ttggtcaact ggtcaaggtt tgcggtgtgg tgctcatcga gataatgcca aatctcgccg
     1801 gccacgattc ctatctccat tatcatgttg gggatcctcc aatcagtgaa tttgaattaa
     1861 accatataac gtaatataac acggaaaaac ttcatgagga agctgtttgt aataaaaaaa
     1921 aacagcgatc atggccgcct ggccagcgcc tcccccaaaa acctccctgt atgggagagg
     1981 ggtgcccccg cgacttcctc cggagacccg cagacgacaa tatttcctcc cgcctgcccg
     2041 ccctccggcc ccagatcaat aaggtaatcc gccattttga tgacatcaag attatgttcg
     2101 atcaccacca ccgtatttcc ctgctggcgc aggtcgaaaa gcgccttcaa aagatttttg
     2161 atgtctgaaa aatgcagacc ggtcgtgggc tcatccagaa gataaagggt ctttccggtc
     2221 gatttcttca ttaactccga ggcaatcttg atccgctggg cctctcctcc cgaaagggtc
     2281 gtcgatgact gccccagttt tacataaccc agtccgattg aatcaagcac ctcaagcttt
     2341 tcgcgaacgt aagaaaactt tgaaaaaaaa tccttcgcct ccaggatcga gaggtctagg
     2401 acctccgcga tgtttttccc ctgataccgg acctcaaggg tggaggcgtt gtaccgttgg
     2461 ccccggcaat cttcacagac cacaaaaagg tccggcataa agctcatctc aagcttctcg
     2521 aaaccaactc cccgacactt ttcacaacgt ccccccttca gattaaaact gaagcgcgac
     2581 tgggtgtagc ggcggagctt tgacgtctca agcgagctga aaagcctcct gatgaaacca
     2641 aaaatgtccg tgtaggtggc ggggttcgat ctcggcgttc gcccgatggg cgactggtca
     2701 atctcaatg
//
LOCUS       DAOSIO010000201         2678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_33123_length_2678_cov_4.240183, whole genome shotgun sequence.
ACCESSION   DAOSIO010000201 DAOSIO010000000
VERSION     DAOSIO010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33123_length_2678_cov_4.240183"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..855)
                     /locus_tag="PK590_07080"
     CDS             complement(151..855)
                     /locus_tag="PK590_07080"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOG24396.1"
                     /translation="MADDTLFFLKSLKEDGIEHLYLTESQPPAATSNSSDPKKLTRES
                     PELSQSVPSQPASSESAKTAILNLRDKVLKCRKCPALVTNRNKIVFGAGNIRAQLVFV
                     GEAPGRDEDLQGIPFVGRAGQLLTKIIEAMGMTRREVFICNVLKCRPPQNRPPAPDEV
                     IHCRPYLKEQLRLIAPKIIVALGNHACQALLSTDKGISQLRGSLQEYEGIPVMCTYHP
                     AYLLRNPAEKRRCGRI"
     gene            complement(857..1975)
                     /locus_tag="PK590_07085"
     CDS             complement(857..1975)
                     /locus_tag="PK590_07085"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG24397.1"
                     /translation="MKIDIIHATAGEGHRKIAIAIQEALTRVGRKDLEIRFLDALDFV
                     SPVMKSSYAPFYHWAVRRAPALWGGSYEVLDRPFVYSLARPFRSLFNSLNAQPLLKNL
                     LEHQPDVVISTHFMAPEILGRARVRGRLRSKILTVITDFFPHTVWTNPGTDHYWVMSE
                     VTKQELFRRGIPPEKITAGGIPVSAAFKPSGNRPEVLKQYGLEQGRFTVLLTSGSFGL
                     GPTEEILDRLNEFKERVQCFVVCARNESLRKKLEEKIYYFPVKIFGFVDCMPDLMEAS
                     DLMIAKSGGSTTSEALAKNLCMVVMSPIPGQETRNAKLLRDSDASFFLQKPSQISGIM
                     QGILSDPSLMVRKQRAIAGLSRPDAADNLVQMVMDGKF"
     gene            complement(1965..2567)
                     /locus_tag="PK590_07090"
     CDS             complement(1965..2567)
                     /locus_tag="PK590_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOG24398.1"
                     /translation="MPQTMNKIVFIDRDGVINVDLWKYVERWQEFRFEPSAPAAMKAL
                     TDAGFDIFIISNQAGVGDGVFTAEQLRDVHEKMIGELTARGVRIKGAHYCTHSKHGNC
                     SCRKPRTGLFEKAVEGVRFKKSETFFVGDKATDIEAGKNFGVRTILVRTGYGAETEKK
                     LTPATRPDYVVDDLGAAADRILAAQADTKSKTDVGGSYEN"
BASE COUNT          592 a          761 c          683 g          642 t
ORIGIN      
        1 cgtcatcctg aaccacatcc ccaacccccg tcatcctgaa cgaagtgaag gatctctcct
       61 tccccaagat gcttcgcttt agctcagcat gacgaccctt gcgctaacca gcgtcatgtg
      121 aggcgttctt gagaaacgcc atcactttct tcatatcctc ccacaccttc ttttttcagc
      181 aggattgcgg agaaggtacg cgggatgata cgtacacatg acgggaatac cctcatactc
      241 ctgcaaactt ccgcgaagtt gggaaattcc cttatcagtc gaaagcagtg cctggcaggc
      301 atgattgccg agcgccacga tgatcttcgg cgcgatcagc cggagctgtt ctttaagata
      361 aggccggcag tgaatcacct catccggcgc gggaggtcga ttctgcggag gccgacattt
      421 gagcacattg caaataaaga cttcccgccg ggtcatgccc atcgcctcga taattttcgt
      481 aagaagctga cccgcccgtc cgacaaaagg gataccttgc aggtcttcgt cacgccccgg
      541 cgcctcgccg acaaaaacaa gttgagcgcg aatatttccg gccccaaaaa caattttgtt
      601 ccggtttgtg acgagggccg gacacttccg gcacttcaaa actttgtccc ggagattcaa
      661 aatcgccgtt ttggcagatt cggatgaagc tggttggctt gggacggatt gtgacagctc
      721 cggggattcg cgcgtcaatt tttttgggtc agacgaattc gaagtcgccg cgggcggctg
      781 tgattcggtc aaataaagat gttctattcc gtcttccttg agtgacttga gaaaaaaaag
      841 cgtgtcgtcc gccatatcaa aatttgccgt ccatgaccat ctggacgaga ttgtccgccg
      901 catcaggcct cgagagtccg gcaatcgccc tttgtttgcg gaccatcagg gacgggtcgg
      961 aaagtatccc ttgcataatg ccgctgattt gcgaaggttt ctgcagaaaa aaagacgcgt
     1021 cgctgtccct gagaagtttc gcgttgcgtg tctcctgccc cggaatcggg gacatcacaa
     1081 ccatgcaaag atttttggcg agcgcttcgc tcgtggtgga accgccggat ttcgcgatca
     1141 tcagatcact cgcctccata agatccggca tgcaatccac gaaaccgaat atcttgacgg
     1201 gaaaatagta aatcttttct tccagtttct tcctaaggga ttcgtttctc gcgcaaacga
     1261 cgaaacactg gacccgttcc ttaaactcat tcagccggtc taggatttct tccgtgggcc
     1321 ccagcccaaa actgccgctt gtcagaagca ccgtaaagcg gccctgctcc agtccgtatt
     1381 gtttcagcac ttccgggcga ttaccggacg gcttgaaggc cgcgctgacg ggaatgccac
     1441 cggccgtaat cttttcgggc gggatccctc gtcgaaaaag ctcttgcttc gtcacttcgc
     1501 tcatgaccca gtaatgatcc gttcccggat tggtccagac cgtatgcgga aaaaaatcgg
     1561 tgatgaccgt cagtattttg gacctcaggc gtccccgtac ccgggcgcgg ccgaggattt
     1621 cgggcgccat gaaatgcgtt gagatgacaa cgtccggctg atgctccagc agatttttaa
     1681 gaagcggttg ggcgttgagg gagttgaaca gggagcgaaa gggacgagcg agtgaataga
     1741 caaacggccg atccaggacc tcatagctcc cgccccaaag cgccggagcc cttcggacgg
     1801 cccagtggta aaagggcgcg taactcgatt tcattacggg gctgacaaaa tccagcgcgt
     1861 ccagaaaccg gatttcgaga tccttccgtc cgacccgcgt caacgcttcc tgaatggcga
     1921 tggcgatttt tcgatgtcct tcaccggccg tcgcgtgaat gatgtcaatt ttcataggat
     1981 cctcccacat ctgttttgct tttcgtgtcg gcctgggcgg caagaatacg gtccgcggcc
     2041 gcgccaagat cgtccacaac ataatccgga cgggtggcgg gggtcaattt tttttcggtc
     2101 tcggcgccgt atccggtccg gacaagaatc gtccgaactc caaaattctt tccggcttcg
     2161 atatccgtag ccttgtcgcc gacaaaaaag gtttcgcttt ttttgaaccg gactccctcc
     2221 accgcttttt cgaataatcc tgttcgaggt ttccggcagg aacagtttcc gtgttttgag
     2281 tgggtgcagt agtgcgcgcc cttaatacgg acgccccggg cggtgagttc cccgatcatc
     2341 ttttcatgaa cgtcccggag ctgctcggcc gtaaagactc cgtcacccac tccggcctgg
     2401 ttggaaataa taaaaatatc aaatcccgcg tcggtcagcg ctttcatggc ggcgggagcg
     2461 ctcggctcaa agcggaactc ctgccagcgc tcgacgtatt tccagagatc aacattaatg
     2521 acaccgtccc ggtcgataaa aactatttta ttcattgttt gaggcatagt cgtttttctc
     2581 attccctata tcgcgatcaa tcgtcatccc gccaccgtca tcatgcgtcg tgtccccaac
     2641 ccccgtcatc ctgaacgaag tgaaggatct ctctttcc
//
LOCUS       DAOSIO010000202         2658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_33432_length_2658_cov_3.997311, whole genome shotgun sequence.
ACCESSION   DAOSIO010000202 DAOSIO010000000
VERSION     DAOSIO010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33432_length_2658_cov_3.997311"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PK590_07095"
     CDS             complement(<1..222)
                     /locus_tag="PK590_07095"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344486.1"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOG24399.1"
                     /translation="MKQPDLKGHFGPYGGRYVPETLVKACEELTEAYVKVRRDPKFKK
                     ELTHYLTSYAGRPTPVYEARNLSAYFKRGR"
     gene            complement(219..992)
                     /locus_tag="PK590_07100"
     CDS             complement(219..992)
                     /locus_tag="PK590_07100"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG24400.1"
                     /translation="MILVRKSRFFLQVAVLWVLVCPGVASAQSPSRFEEANTCYRTGD
                     FEKSAELFEALVRDEPGHPVFLYNLGNAYVRTGKLSQAILAFEKALLASPRNTDVRQN
                     LEYARSLLEYRVDDDRNWYLKVTEVALSYFTQLEANFLALVFLALFLLVGSFYFIFKQ
                     PVFWGMGRKILVVFVIATGALAGLKHVQVDMIRPAIIMANECNVRYGPSEHDQIAFKM
                     GEGLKVFIMDRRADWSRVLLTNGENGWVHHEDISEITLT"
     gene            complement(989..>2658)
                     /locus_tag="PK590_07105"
     CDS             complement(989..>2658)
                     /locus_tag="PK590_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOG24401.1"
                     /translation="VEFNYTLIPNTPGRFTILPIQVQVGVQPYATEPITVEVMGASGQ
                     VPPQTTTQQAPVAASPHPMTGYGQPVQPVAQSPQPVLSQTEDDNIFVRAALDKKSVYQ
                     NEQLLLSYSLYTRYDTRYEGFEKEPETSGFWIEDFPMEREIARETVRVGGKKYVKADV
                     KKMALFPTSPGNYTIQPGSLKVSIREEPQSSSVFDEFFSDSFFTGGSFFARRQNRLLN
                     PSPLQLVVKPLPEDGKPGSFQGAVGQFRISASLDREKVKQNEPVTLKMTIEGEGNIET
                     ITRPKVPELTGFKTYDADTSSQLFKTGNVIGGTKSFEVVFIPQNDGKAYIPPLEFSYF
                     DPRAEKYVTVRTPNFQIEVEKSEQAFKVPDAIPEKTLFQKKVTLESKDICFIEERLPN
                     PIPGKVFKLLYRGLQVLNILLALVLVALFVKNREETIFSKDEGLKRRKQARRVAQSNM
                     KKLNRLARSSTAKGTEKFFVELERSLTQYISDRWNLSTYGVTRKDLEDALAQHLGAED
                     PLSKELIQLYELCDESRFGKGLIPQASKKIGMKIFQDTVNRLERIRL"
BASE COUNT          667 a          746 c          605 g          640 t
ORIGIN      
        1 ccgtccgcgc ttaaaatacg cgctcaaatt cctagcttcg taaactggag tcggccggcc
       61 ggcataggaa gtaagataat gtgtcagttc ctttttgaat tttggatctc gacgaacttt
      121 cacgtacgcc tccgtcaact cctcacaggc cttcacaaga gtttcaggaa cataccggcc
      181 cccgtagggt ccaaaatgtc ctttcagatc aggctgtttc atgtcagcgt aatctccgaa
      241 atatcctcat gatgcaccca tccattttcg ccattggtca gaagcacccg actccagtcc
      301 gcgcgacggt ccataatgaa aaccttgagc ccttcgccca ttttaaacgc aatctggtca
      361 tgttcggacg gcccataccg cacattgcat tcattcgcca taataatcgc cggacgaatc
      421 atgtccacct gaacgtgctt caaaccggcc aacgcgcccg tcgcgataac aaacacgacg
      481 aggattttcc ggcccattcc ccaaaaaaca ggttgtttga aaatgaagta aaacgatccc
      541 accaaaagaa aaagggcgag aaacaccagc gccagaaaat tagcttccag ctgagtaaaa
      601 tatgagagtg caacctccgt cactttcaga taccagttac ggtcgtcatc cacccggtat
      661 tcaagtaaac ttcgcgcata ttccagattc tgacgaacgt ctgtattccg cggtgaggcc
      721 agaagggcct tttcaaacgc aagaatggcc tgacttaatt tccccgtcct gacatacgcg
      781 ttccccagat tgtatagaaa aaccggatgg cccggctcat cacgaaccag ggcttcgaac
      841 aattccgccg atttttcaaa atctccggtc cggtagcacg tattggcttc ctcaaaacgg
      901 ctcgggctct gtgccgacgc caccccgggg caaaccaaaa cccacaaaac ggccacctgc
      961 aagaaaaaac gggattttcg aaccaagatc atagccgtat cctttccagc cggttgaccg
     1021 tatcctgaaa gattttcata ccaatctttt tggaggcctg ggggatcaac ccctttccga
     1081 accgggactc gtcacatagc tcgtaaagct ggatcaactc cttggaaagc ggatcctcag
     1141 cccccagatg ttgagcgagc gcatcttcaa ggtctttccg ggtcactccg taagttgaaa
     1201 ggttccaccg atccgaaatg tattgcgtga gtgaacgctc aagctcgaca aagaacttct
     1261 ccgttccctt cgccgtggat gatcgcgcga gacgattgag tttcttcatg ttagactgcg
     1321 ccacccgtcg ggcctgtttg cggcgtttca gaccttcatc ttttgaaaaa attgtctcct
     1381 cgcgattctt cacaaaaaga gcgaccagca caagggccag gagaatattc aaaacctgca
     1441 atccccggta aagcagttta aaaacttttc cgggaatcgg attcggcaat cgttcctcga
     1501 taaaacagat atccttggat tccagcgtca cctttttctg aaacagggtc ttttccggaa
     1561 tcgcgtctgg taccttgaat gcctgttccg atttttcaac ttcaatctga aagttcgggg
     1621 tgcggaccgt cacgtatttc tcggccctgg gatcaaaata actgaactca agcggcggaa
     1681 tataagcctt cccatcgttc tgaggaataa aaacaacttc aaaactcttg gtccctccaa
     1741 tgacatttcc cgttttaaac aactgagagc tggtgtccgc gtcgtaagtt ttaaaacccg
     1801 tcaactccgg caccttgggg cgcgtgatcg tctcgatgtt gccctccccc tcaatcgtca
     1861 ttttaagggt cacaggctca ttttgtttta ccttctcccg gtccaggctg gccgaaatac
     1921 gaaactgtcc gactgctccc tgaaaactgc ccggtttccc gtcttccggc aaaggtttca
     1981 cgacaagttg aaggggtgac ggattcaaaa gccggttttg ccgccgggcg aaaaaggatc
     2041 cccccgtaaa aaaactatcg gaaaaaaact cgtcgaacac gctggagctc tgcggttctt
     2101 cacgaattga caccttgaga cttcccggct gaatggtgta gttgccgggc gaggtcggga
     2161 acaaggccat ttttttcacg tccgccttga catatttttt ccctccaacc cgcacggtct
     2221 cacgggcaat ctcccgttcc atcggaaaat cttcgatcca aaaaccactc gtttccggtt
     2281 ccttttcaaa cccctcgtag cgggtgtcat atcgcgtgta aagagagtaa gacagcaaaa
     2341 gctgttcatt ttgatacacg ctttttttat caagcgcggc ccgaacaaaa atattgtcgt
     2401 cctctgtttg cgacagaacc ggctgaggcg attgcgcgac cggctgaaca ggttgtccat
     2461 accccgtcat gggatgaggc gaggccgcca cgggagcctg ctgagtcgtc gtttgagggg
     2521 ggacctgtcc cgaggccccc ataacttcca ctgtaatcgg ttcggtagcg taaggctgga
     2581 ccccaacctg aacctggatg ggcaatatcg tgaaacgacc cggagtattg gggatcaggg
     2641 tatagttgaa ttccacac
//
LOCUS       DAOSIO010000203         2657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_33452_length_2657_cov_4.595696, whole genome shotgun sequence.
ACCESSION   DAOSIO010000203 DAOSIO010000000
VERSION     DAOSIO010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33452_length_2657_cov_4.595696"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PK590_07110"
     CDS             <1..703
                     /locus_tag="PK590_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24402.1"
                     /translation="PDSVESPIQKFFDSGRIGKVIETTGARAGDIIFLVADQFKVACV
                     ALGALRCFLAEKYGMLPEDKFELLWVVDFPLFEWNEEEQRCDACHHPFTSPKPEDVPL
                     LDTEPLKVRARAYDIICNGTELGGGSIRIHDRKVQSKMFELLKLSPEQAEAQFGFLLK
                     ALTFGAPPHGGLAIGLDRFVTLLLARDSIREVIAFPKTQKGTCLMTGAPSHATERQLK
                     ELNLDAKKMWIEKPK"
     gene            779..1735
                     /locus_tag="PK590_07115"
     CDS             779..1735
                     /locus_tag="PK590_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOG24403.1"
                     /translation="MQKIIKIQSNEEAAAVYGNLDRNLRQAEKDYRVRLIAKNDRLKI
                     LGDKKDVEDAYDHFLRLIHGYRSGGHESGRTPPDSRGVRNERGEAAPKGGIHFFHRGK
                     VVQAKTENQKAYLQAIQSKDIVISIGPAGTGKTYLAMAAAVEALKKKYVGRIILTRPA
                     VEAGESLGFLPGDLAAKVNPYLRPLYDALYDMLEYEEVTRYTERRAIEIAPLAYMRGR
                     TLNDSFVILDEAQNCTHSQMKMFLTRMGQTSKVVITGDITQVDLPAGKKSGLIEIRKI
                     LADIPDIQFIYLNETDVVRHPLVQEIIKAYEKYDRKKAQNES"
     gene            1695..>2657
                     /locus_tag="PK590_07120"
     CDS             1695..>2657
                     /locus_tag="PK590_07120"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24404.1"
                     /translation="MKSTTAKKRKTNPEMVLRRRAGAKLVPFGIFLLAGALIALILFW
                     NQPWRFEQQEFVQDEPAPRSFFSPVSFSYVDEAKTEVLRLEKQKNSPPVYRVDPVLTA
                     NIRQLAESFFIALQEIRSEKEKTGTLPQKELPIHLPSFSLESLLSAKDIAQVQTVTLS
                     ALNAYLLRGMVPELEKQGIMDSGRDTVLHVAEDPRQETVVLTTQLLTVEEAVRESFSV
                     LPSEIFNQDAGLRTAAAEVLRGLLAANLVYDEEKTREHRKKLSAAVPPVEIRVKKNEL
                     IVQRGLLVTKDVKEKLDQVQKKISSRKQKIQIVAGSFIILLLYGL"
BASE COUNT          617 a          697 c          776 g          567 t
ORIGIN      
        1 gccggattcg gtggaatcgc cgatacagaa attttttgat tcgggacgga tcggaaaagt
       61 aatcgaaacg acgggcgcgc gggcggggga tattattttt cttgtggcgg accagttcaa
      121 ggtggcctgc gtggcgctcg gggcgcttcg ctgttttctc gcggaaaagt atggtatgct
      181 tcctgaagat aaatttgagt tgctctgggt cgtggatttt ccgctttttg agtggaatga
      241 ggaagaacag cggtgtgacg cgtgtcatca tccgttcacg tcacccaagc cggaggatgt
      301 gccgttgctt gacacggagc cgctcaaggt acgggcgcgg gcctatgaca tcatttgcaa
      361 cgggacggag cttggcggcg ggagcattcg tatccacgac cggaaagtcc agtcaaaaat
      421 gtttgagctt ttgaaacttt ctccggagca ggccgaagcc cagttcggat ttttgctgaa
      481 ggcgctgact tttggcgcgc cgccgcacgg ggggctggcg atcggactgg atcgttttgt
      541 gacgctcctg ctcgcgcgtg attcgatccg ggaggtgatc gcgttcccca aaactcagaa
      601 ggggacatgc ctcatgacgg gtgccccttc gcacgcgacg gagcgacaac ttaaagaact
      661 taatcttgac gcgaaaaaga tgtggattga aaaacccaaa taatgtccgg tgtttggcgg
      721 gcggagcttg gaatttcgga agttggtacc ccgtcccctg aaccccagaa agggcccctt
      781 gcagaaaatt atcaaaattc aatcaaatga agaagccgcc gcggtgtacg gaaaccttga
      841 ccggaatctc cgccaggcgg aaaaagacta tcgcgttcgc ctgatcgcca aaaatgaccg
      901 gctgaaaatt ctgggtgaca aaaaagacgt cgaggacgct tacgatcatt ttctccggct
      961 gattcatggc taccggagcg gaggacatga gtctggtcgg accccgccgg attcccgcgg
     1021 ggttcggaac gagcgggggg aagccgcgcc caagggaggg attcattttt tccaccgtgg
     1081 caaagtagtc caggccaaaa ctgaaaacca gaaggcctat ctgcaggcga tccagtcgaa
     1141 ggacatcgtc atttcgattg gccccgcggg gaccgggaaa acgtatctcg ccatggcggc
     1201 ggcggtggag gccctcaaga agaaatatgt cgggcgaatc atcctgacgc ggcccgccgt
     1261 cgaggcgggc gagagcctcg gatttttgcc cggtgatctc gccgcgaaag tcaatccgta
     1321 tctgcggccc ctttatgacg ccctctacga tatgcttgaa tatgaggaag tcacccgcta
     1381 caccgaacgg agggctattg agatcgcccc gctcgcctac atgagaggac gcacgctcaa
     1441 tgactctttt gtcattctgg atgaagcgca gaactgcacc cacagccaga tgaaaatgtt
     1501 tctcacgcga atggggcaga cctcgaaagt cgtgattacg ggtgacatta cccaggtcga
     1561 tcttccggcg gggaaaaagt ccggcctgat cgagatccga aaaatccttg ccgacattcc
     1621 ggacatccag tttatctacc tgaacgaaac ggacgtcgta cggcatcccc tggttcagga
     1681 gatcatcaag gcctatgaaa agtacgaccg caaaaaagcg caaaacgaat cctgaaatgg
     1741 tgttgcggag gcgggcgggc gcgaagcttg tccccttcgg gatctttctt ctcgcgggag
     1801 ccctgatcgc cctgatcctt ttctggaatc agccctggcg gtttgaacag caggagtttg
     1861 tgcaggatga gccggccccc cgatcctttt tttcgcccgt ttcattttcc tatgtggatg
     1921 aggcgaagac cgaggttttg aggctcgaga aacaaaaaaa cagcccgccc gtttacaggg
     1981 tcgatcccgt cctgacggcg aacatacgtc agctcgccga gagctttttc atcgcgctcc
     2041 aggaaatcag gagcgaaaaa gagaagaccg ggacactccc gcagaaagaa ctcccgatcc
     2101 atctcccgtc cttttctctg gagtccctgc tttcggccaa agacatcgcc caggtccaga
     2161 ccgtgacttt gtcggccctg aacgcctatc ttttgcgggg tatggttccg gagttggaga
     2221 aacaggggat catggattcc ggccgcgaca cggttctcca cgtcgcggaa gacccgcgcc
     2281 aggaaacggt ggtattgacg acgcagctat tgacggtcga ggaagctgtc cgggaatctt
     2341 tttccgtatt gccctccgag atttttaacc aggatgccgg tctcagaacg gcggccgcgg
     2401 aggtgctcag ggggctactt gccgccaatc tggtttatga tgaggaaaaa acccgtgagc
     2461 accggaagaa actttcggcg gcggtccctc cggttgagat ccgggtcaag aaaaatgagc
     2521 tgatcgtcca gcgcggcttg ctcgtgacca aggacgttaa ggagaaactg gatcaggtcc
     2581 agaaaaaaat ctcgtcacga aaacagaaga ttcaaatcgt cgcgggatcc tttatcatcc
     2641 ttctgcttta cggcctc
//
LOCUS       DAOSIO010000204         2642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_33699_length_2642_cov_3.275222, whole genome shotgun sequence.
ACCESSION   DAOSIO010000204 DAOSIO010000000
VERSION     DAOSIO010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33699_length_2642_cov_3.275222"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1309)
                     /locus_tag="PK590_07125"
     CDS             complement(53..1309)
                     /locus_tag="PK590_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG24405.1"
                     /translation="MGNDQTRTLQTSNPNGLNVLFLSHIRVNSIYERGIFTDLARFFM
                     KQGHRVHFVAPVERMFKKPTHLIREENSSILKVKTLNLLKMNFFEKGIGALLLGRQYN
                     IAIKKYFAGVRYDIILYSPPFVALQNVVREIKERDQAKTYLLLTDFFPQSAIDLGVFS
                     EKNILCKYFRAQEKKLYQLADHIGCMSPAGAKYVVERKNLDKTKVEVCPNSAEIDGKD
                     PFTNKNREVLRRYNIPSHTINYILGGNLGKPQGLDFLIDIIQSRKNDLSSYFIIVGNG
                     TEYGWIKKWMDYKKPVNAMLMPGLRQKDYTELVRACDVGLIFLDKRFTVPHFPSRLVS
                     YLENGLPILAATDLSTDLGEIAEKNHFGFSCINGDLDRFFAHLDWFSKNRSQMAEMGK
                     NGYNFLLKNYTVSQAYNIIMKHFMKP"
     assembly_gap    1408..1417
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1515..2540)
                     /locus_tag="PK590_07130"
     CDS             complement(1515..2540)
                     /locus_tag="PK590_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG24406.1"
                     /translation="MPTKYEMVKRQVQKKPLRWAISGVAGFIGSNLLEQLLKLDQRVT
                     GVDNFLTGYRRNLEDVRSLVTKKQWARFRFFEGDIRNLGDCRKACQGADFVLHQAALG
                     SVPRSLKVPLATHEHNITGTLNMLVAARDCGVKRLVYASSSSAYGDHPGLPKVESRIG
                     NPLSPYAVTKYVNELYAGVFFRCYGLESIGLRYFNVFGPRQDPGGAYAAVIPRWIRQL
                     RDKERPVIYGDGKTSRDFCYVDNAVQANILAAVTENKKAVNEVYNVSVGNKTTLDDLF
                     FMIRSFLTMYDEKIKNIRPIKNPFRPGDIRHSQANISKAKTLLGYRPLYTVLTGMKQT
                     IQWFIAR"
BASE COUNT          729 a          629 c          503 g          771 t
ORIGIN      
        1 ccactttaac agtccaccct ttttaaaaaa agctaaacgg cttcgctagt ggtcatggct
       61 tcataaaatg tttcattatt atattatacg cttgtgaaac ggtataattt ttcaataaga
      121 agttataacc attcttcccc atctctgcca tctgggatcg atttttgcta aaccaatcca
      181 gatgggcaaa aaatctatcc agatcaccgt tgatacatga aaatccgaaa tggttctttt
      241 cggcgatctc gccaagatcc gtgctaagat ccgttgccgc gagaattggc aaaccgtttt
      301 ccagataaga taccagcctg ctgggaaaat ggggaacagt aaagcgcttg tctaaaaata
      361 ttaatcctac atcacacgcg cgaaccagtt cagtataatc tttttgacgt aagccgggca
      421 tcagcatcgc attcactggt tttttataat ccatccattt cttgatccag ccatattcgg
      481 tgccatttcc cacaattata aaataggatg ataaatcatt ttttctggat tgaatgatat
      541 caattaaaaa atcgagcccc tgcggttttc ccaggttacc tcctaatatg taattgatcg
      601 tatgggacgg aatgttataa cgtctcaaaa cttcccggtt cttgtttgtg aagggatctt
      661 tcccatcaat ttcggcgcta ttaggacaaa cttccacctt tgttttatcg agatttttcc
      721 gctccaccac gtattttgca cctgcgggag acatacaacc gatgtgatca gccaactggt
      781 acaacttctt ctcctgcgct ctaaaatatt tacacaagat gtttttttca gaaaagacac
      841 ctaaatcaat agcgctttgg gggaaaaagt ctgtcaacaa cagatatgtt ttcgcctggt
      901 ctctctcttt aatctctctg actacatttt gcaacgccac aaaaggagga ctgtaaagaa
      961 taatgtcgta gcgaacccct gcaaaatatt tctttattgc aatattatat tgccgcccca
     1021 gcaacaacgc tcctattcct ttttcaaaaa aattcatctt cagcaaatta agcgttttaa
     1081 ccttcaaaat ggagctattc tcttcccgaa tcaaatgtgt tggcttttta aacattcgct
     1141 caacgggggc aacaaaatgc accctgtgcc cttgtttcat aaagaacctg gcaagatccg
     1201 taaaaatgcc tcgttcgtat atgctattaa cacggatatg cgacaaaaaa agcacattca
     1261 acccattggg attggatgtt tgtagagtgc gagtttggtc gtttcccata tttcagatat
     1321 tttgtttgtg ttagcgtttt tcccgtaagg gaatgaatgc cgagtttctt ctgtttgtat
     1381 tattgcgatg tttccttaaa aacgctgnnn nnnnnnngca ttaactgcgg cactcatcaa
     1441 gcgacattga aacaatctca taagtcgcta ctgccccccg aaaaatatcg gatggtcaaa
     1501 acagccttga ctgactatcg ggcaataaac cattggatcg tctgcttcat tccggttaga
     1561 accgtataaa gcggtctata tccaagcaat gttttcgcct tcgagatatt cgcctgagag
     1621 tggcgtatgt cgcctggccg aaaagggttt ttgattggcc ggatattttt tattttttca
     1681 tcatacatcg tcagaaaact tcggatcatg aaaaacagat catcaagcgt tgtcttgttc
     1741 cccaccgaaa cattataaac ttcgttaacg gcttttttat tttcagtcac tgccgcaagg
     1801 atatttgcct gtactgcgtt atcaacataa caaaaatccc ggcttgtttt tccatccccg
     1861 taaataaccg gacgctcctt atcacgcagt tgtctgatcc atcgggggat caccgccgca
     1921 taggctcccc caggatcctg ccgggggcca aacacgttaa agtaacgtaa tccaatactt
     1981 tctaacccat agcaacgaaa aaaaacaccg gcataaagtt cattgacata ctttgtaacg
     2041 gcatagggtg aaagggggtt cccaatcctg ctttccactt tggggagtcc cgggtgatcc
     2101 ccgtaagcag aactgctgga agcatacacc aaacgtttta ccccgcaatc tcgtgccgca
     2161 accagcatat taagtgttcc cgtaatattg tgttcatgcg tcgcaagagg aactttgagc
     2221 gagcgaggca cagaacctag cgctgcctga tgcaacacaa aatcagctcc ctgacaagct
     2281 tttcggcaat ctcccagatt ccggatatcc ccttcaaaaa accgaaatcg ggcccattgt
     2341 ttttttgtga caagcgacct gacatcttcc agatttcgcc ggtaacctgt caggaaatta
     2401 tcgactccgg taactcgctg atccagctta agcaattgtt caaggagatt ggaaccgata
     2461 aaaccggcaa ctccactgat ggcccatcgt aaaggtttct tttgaacttg ccttttgacc
     2521 atttcatatt tggtcggcat cggatctacg cccttcggat tttagaactt cgaatgcccc
     2581 gctggccaaa ggcatttcgg gtatcaagaa tgcaggtagc atttttctca atcatctcgt
     2641 ag
//
LOCUS       DAOSIO010000205         2624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_34001_length_2624_cov_3.661736, whole genome shotgun sequence.
ACCESSION   DAOSIO010000205 DAOSIO010000000
VERSION     DAOSIO010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34001_length_2624_cov_3.661736"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PK590_07135"
     CDS             <1..553
                     /locus_tag="PK590_07135"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOG24407.1"
                     /translation="ALLFLFIRHRWFSVTAQEIREVVRLTARYPRDRAKTLAKRLFQK
                     NIVVYGGGLMQPVALRWRTQFAENAKTLVSCEALPEMFHHEVEGWIFPRFKVNRSVAI
                     FLADKNEPGWLGKKKKVAMRAIREHGAEVIEIKASGSGVLGRIFSMVMLGDWTSCELA
                     NLYKIDPMAICVIDQIKKAVRSV"
     gene            653..1390
                     /locus_tag="PK590_07140"
     CDS             653..1390
                     /locus_tag="PK590_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOG24408.1"
                     /translation="MKVVLLCAGYATRLYPLTENQPKPLLPVGGRPILEWILERVSKV
                     EEVESVYLVSNDKFAGHFETWAKHAQYPWPVDVINDRTSSNETRLGAIGDLAFVIRAR
                     NLEPDDLMVIAGDNFFDFDLGSFVKYGNKVRPHGVIAVYDVKDKELAKRYGLVRTRED
                     GRVVEFQEKPEEPQTTLASSGIYWLPKEIWGFLDTYIKGGHNTDQPGHYMRWLAEKSG
                     LYAFPLEGKWLDIGDLASYQKANDIVN"
     gene            1446..>2624
                     /gene="metK"
                     /locus_tag="PK590_07145"
     CDS             1446..>2624
                     /gene="metK"
                     /locus_tag="PK590_07145"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOG24409.1"
                     /translation="MEKGNYFFTSESVSEGHPDKVCDQVSDAILDAYLAQDPSSRVAC
                     ECLATTDRMTIAGEITSKGKVDAEKVARHVIKKIGYVDSTIGFDYKTCKIDVALHTQS
                     PDIAMGVDTGGAGDQGMMFGYATNETQELMPLPIMMAQKLVRYLTAVRKSGKIKYLRP
                     DSKSQVTIEYKNEKPVRVEAVVLSSQHDGDVGLSTIQKDLTEKVIKKVVPAELLDSKT
                     KIYINPTGRFEVGGPHGDTGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYFA
                     RYVAKNIVAAGLAGSCEIQVSYAIGVAKPVSIRVDTFGTNRISEEAIVDAVQKVFDFT
                     PRSIIKTLDLLRPIYHKTAAYGHFGRSEAGFTWERTDKVDALRKVLKLKEGCSCCKWT
                     V"
BASE COUNT          654 a          587 c          752 g          631 t
ORIGIN      
        1 cgcgcttctt ttccttttta tacggcatcg gtggttttcc gtgacagcgc aagaaattcg
       61 ggaagtggtc cgtctgacag cacggtatcc cagggatcgg gcaaagacgc tggcaaaaag
      121 actttttcag aaaaatattg tggtttacgg cggaggtctg atgcagccgg ttgcgttgcg
      181 ttggcgcacc caatttgcgg aaaacgcaaa aacccttgtc tcctgtgagg ctttgcctga
      241 aatgttccat catgaggtgg aagggtggat cttccccagg tttaaggtga atcgttcggt
      301 ggccattttt cttgcggaca agaatgaacc gggttggcta gggaaaaaga aaaaagtcgc
      361 gatgcgcgcc attcgcgaac atggcgccga ggtcatcgag atcaaggctt ccggcagtgg
      421 tgttctgggg agaatttttt ccatggtcat gctgggagat tggacgagct gtgagctcgc
      481 gaatctctac aagattgatc ccatggcgat atgtgtcatt gatcagatca agaaagcagt
      541 tcgctccgtt tagaccgcag gtcgcgggat tgttgcgggc ggatcgcgga tgaaacaact
      601 ggctgtaagt gtgcaaatac tttgaaacgc tgatacgagg ttaaaaaaga ctatgaaagt
      661 tgttttgctt tgcgcgggat atgccacccg tctctatccg ttgactgaaa atcaaccgaa
      721 acctcttctg cccgttgggg gacgccccat tcttgagtgg attctcgagc gggtttcgaa
      781 agtggaagag gtggaatccg tctatctcgt cagcaatgac aaatttgcgg gacattttga
      841 aacctgggcg aaacacgccc aatatccctg gcctgttgat gtgataaatg accgcacctc
      901 cagcaacgag acccggctgg gcgcgatcgg ggatcttgcg tttgtgattc gcgcgagaaa
      961 tcttgagccg gacgatctca tggtcattgc gggggataat tttttcgatt ttgatctggg
     1021 ttcttttgtg aaatacggaa acaaggttcg gcctcacggg gtgattgctg tttacgatgt
     1081 aaaagataaa gaacttgcga aacgctacgg attggtgaga acccgggagg acgggagggt
     1141 tgttgagttt caggaaaaac ccgaggagcc acagacgacg ctcgcttcta gcggtatcta
     1201 ttggctcccg aaggaaatct gggggtttct tgacacttat atcaagggag gtcataatac
     1261 ggatcagcct ggacattata tgcggtggct cgccgaaaaa tcgggccttt acgcgtttcc
     1321 cctggagggg aaatggttgg acatcggtga tctcgcctcc taccagaaag cgaatgacat
     1381 cgtcaattaa gatcccgcag aaaattcatt ctggggaatc aattcaatca agaaggagta
     1441 acaaaatgga aaaagggaat tatttcttca cctccgaatc ggtctccgag ggacatccgg
     1501 ataaggtttg tgaccaggtt tcggacgcca ttctcgacgc ctatctggcg caggacccga
     1561 gttcccgtgt cgcctgcgaa tgtctggcta ccacggaccg gatgacgatt gccggtgaaa
     1621 ttacgagcaa ggggaaagtt gacgctgaaa aagtggcccg ccatgtcatc aaaaaaatcg
     1681 gatatgtgga ttccacgatc ggttttgatt acaaaacctg taaaattgat gtcgcccttc
     1741 atacgcaatc tccggatatc gcgatgggcg tggacaccgg tggcgccggc gatcagggta
     1801 tgatgtttgg ttacgcgacg aatgaaacac aagagttgat gccgcttccg atcatgatgg
     1861 cgcagaagct ggttcgttat cttacggcgg ttcgcaaatc cggaaagatc aagtatctcc
     1921 ggcccgattc aaaaagtcag gtcaccatcg aatacaaaaa tgaaaaaccg gtccgcgtgg
     1981 aggctgtggt gctttcctcc caacatgacg gggacgtggg tctttctacc atccagaagg
     2041 acctgaccga aaaagtaatc aagaaagtgg ttcccgcgga attgctggat tcaaagacca
     2101 aaatttacat caatccgacc gggcgttttg aagtgggcgg accgcacggt gatacggggc
     2161 ttacgggccg caagattatc gtggacacct acgggggtat ggggcgtcac ggaggtggcg
     2221 cttttagcgg aaaagatccg tccaaagttg accgctctgc cgcttatttc gcgcggtatg
     2281 tggcaaagaa tattgtggcc gcaggtcttg ccggaagttg tgaaatacag gtttcctacg
     2341 cgatcggtgt cgccaaacct gtttcgattc gagtcgacac ttttgggacc aaccggatca
     2401 gcgaggaagc gatcgtggat gctgtgcaaa aagtttttga ctttacgccg agaagcatta
     2461 tcaaaacgct tgatcttctc cgcccgatct atcacaagac ggccgcctac ggacatttcg
     2521 gacgttccga ggccggattt acctgggaga gaacggacaa ggtggatgcc ctccggaaag
     2581 ttttgaagct taaggagggg tgttcttgtt gcaaatggac agtg
//
LOCUS       DAOSIO010000206         2607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_34321_length_2607_cov_3.496473, whole genome shotgun sequence.
ACCESSION   DAOSIO010000206 DAOSIO010000000
VERSION     DAOSIO010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34321_length_2607_cov_3.496473"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1566
                     /locus_tag="PK590_07150"
     CDS             <1..1566
                     /locus_tag="PK590_07150"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOG24410.1"
                     /translation="TEQLTFVCHPDKLTAGPNNNWMDPKEAKEKMKKLFDGCMKGRTM
                     YVMPYMMGHPKSPYAKACVQITDNSYVAISMRLMTRMGKEALDRIGKSEDFVRGLHSV
                     GDFNPERRFIMHFPQENLVQSFGSGYGGNALLGKKCFALRIASCIGKREGWLAEHMLV
                     MGIQDPHGKTSYIAAALPSACGKTNLAMLQSTLPGYKIWTLGDDIAWLNVGPDGRLYA
                     INPEAGFFGVAPGTSLKTNPNMVKTLKNNKFFPTLYTNTGLDTQNEEPWWEGLDGEIP
                     SQLLDWRGDSWDKSKTTKCAHPNSRFTASIYNCPNLSKEFDNPKGVPIDAILFGGKRA
                     QLIPLVAEAFDWQHGVFIGTRMGSEITAAATSLTVGAIRRDPMAMLPFCGYNMGDYFA
                     HWLDIGRRLKQPPKIYSINWFRTDEHGQYIWPGFGDNIRVLKWIIDRVHGHVDAKETP
                     LGLVPFSKDLDLSGLAISPEHAEKLFEVNPGEWTQELAEIEKYFQSFGSSLPEAIWSE
                     YRRLADKLAAAKR"
     gene            complement(1648..>2607)
                     /locus_tag="PK590_07155"
     CDS             complement(1648..>2607)
                     /locus_tag="PK590_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOG24411.1"
                     /translation="KIMEKVKAPDPETERIEEAKKMLATKRVEPPRSSAANRPGGETA
                     KKRVSFVRHDNVIPRPPFMGYRVLKNVRLQDVFPWINKKSLYTVQWQFRQGDRTERMF
                     DLFLEENVEPVFERFTQQCIREKILEPKVLYGYFPCCSEGDDLIILDESGKIERTRFH
                     FPRQKRDPYLCIADFFRSKASGLMDVVGFMVVTVGHRIREVEEKAFKGHRYQEYLYLH
                     GLSVVTTEALAEYSHAMIRRELKIHLQDKNTKWEIFRQGYQGSRYSFGYPACPHLEGQ
                     RKLFSLFPAEKIGVSLSETLQMVPEQSTSAIIVHHPQAKYFSV"
BASE COUNT          650 a          737 c          632 g          588 t
ORIGIN      
        1 accgaacaac tcacttttgt ttgtcatccg gacaaattaa ccgccggccc caacaacaac
       61 tggatggacc ccaaggaagc caaagagaaa atgaaaaagc tctttgacgg gtgcatgaag
      121 gggcgaacca tgtacgtcat gccctacatg atgggacacc ccaagtcccc ctacgccaag
      181 gcctgtgtcc agatcacgga taattcttac gtcgcgatca gcatgcgcct gatgacccgg
      241 atgggcaaag aagcactgga tcgcatcggc aagtccgaag actttgtccg cggtcttcat
      301 tcggtaggtg atttcaaccc tgaacgtcgg tttatcatgc atttccctca ggagaatctc
      361 gttcagagtt tcggatccgg ttacggcggc aatgccttgc tcggcaaaaa atgttttgcc
      421 ttacgtatcg cgtcctgcat cggaaaacgc gagggctggc tcgccgaaca catgttagtc
      481 atgggaatcc aggaccccca tgggaaaaca agttatatcg ccgccgcgtt accttccgcc
      541 tgcggcaaaa caaacctcgc catgctccag tcaacacttc ccgggtacaa gatctggacc
      601 ctcggcgacg atatcgcgtg gctcaacgtg ggaccggacg gtcgtcttta cgcaattaat
      661 ccggaagccg gtttttttgg agtggcgccg gggacatcgc tcaagacaaa tccgaacatg
      721 gtcaagaccc tgaaaaacaa caagttcttc ccgacccttt ataccaacac cggccttgac
      781 acccaaaacg aggaaccttg gtgggaaggt cttgacggcg aaattccctc ccagcttctg
      841 gactggcggg gtgacagttg ggataaatcg aagacgacaa aatgcgccca tccgaattcg
      901 cgatttaccg cctcaatcta caactgtcct aatttgtcca aggaattcga caatccgaaa
      961 ggggtgccga tcgatgccat cctcttcggt ggaaaacgcg cgcaactgat ccctctggtg
     1021 gcagaggcct ttgattggca acacggtgtt ttcatcggaa cccggatggg ctccgaaatt
     1081 acagccgccg ccacctcgct caccgtcggg gcaatccgcc gcgatcctat ggctatgctc
     1141 cccttctgcg gctacaacat gggagattat ttcgcgcact ggctggacat tggccgcaga
     1201 cttaaacaac cacctaaaat ttattccatt aactggttcc gaacagacga acacggtcaa
     1261 tacatctggc caggtttcgg ggacaatatt cgggtgttaa agtggatcat agatcgggtc
     1321 cacgggcatg tcgacgcgaa agagacgcct cttggcctcg ttcccttttc caaggacctc
     1381 gacctttcgg ggctggccat ctcgccagaa cacgcagaaa aacttttcga ggttaacccc
     1441 ggggaatgga cccaggaact tgccgagatt gaaaagtatt tccagagttt tggctcaagc
     1501 cttcccgaag ccatctggtc ggaataccgc cgtcttgccg acaagctcgc cgcggcaaaa
     1561 cgctgatcag atcgcgtatc ccgcatcaca agtcttgtag aaaaagacgc ttctcgcgga
     1621 acatttcatt tggcgcgtgt cacgaagtta aacgctgaag tatttcgcct ggggatggtg
     1681 aacaatgatc gccgaggttg attgttccgg taccatttga agggtttcag acaggctgac
     1741 cccgattttt tccgccggga acaaactgaa aagttttcgc tgtccctcca gatggggaca
     1801 cgccggatat ccgaaactgt accgtgatcc ctgatacccc tgcctgaaaa tttcccactt
     1861 ggtatttttg tcttgaagat gaattttgag ttctctgcga atcatcgcgt gagaatattc
     1921 cgccaacgcc tcggtcgtca cgacggaaag accatggaga taaaggtatt cctggtaacg
     1981 gtgacctttg aaagcctttt cctccacctc acgaatacga tgccccaccg tgaccaccat
     2041 aaacccaacc acatccatca gccccgaggc tttggagcga aaaaaatcag caatgcaaag
     2101 gtaaggatcc cgcttctgcc gcggaaaatg aaagcgggtt cgctcaattt ttccgctctc
     2161 gtccagaata atcaaatcat ccccttccga acagcaaggg aaatagccgt ataaaacttt
     2221 gggctcaagg atcttttccc gaatacattg ctgggtaaaa cgctcaaaaa caggctccac
     2281 gttctcctcc agaaaaaggt cgaacatccg ctcggtgcga tccccttgcc ggaactgcca
     2341 ctgaaccgta taaagcgact ttttgttgat ccagggaaag acatcctgca accggacatt
     2401 tttcaaaacg cgatagccca taaaaggcgg ccggggaatg acgttgtcat ggcgcacaaa
     2461 actaacgcgt tttttcgccg tctcaccccc ggggcgattc gcggcggacg aacgaggcgg
     2521 ctccacccgt tttgtcgcca gcattttttt cgcctcctca atccgttcag tctccgggtc
     2581 gggcgccttg accttttcca tgatctt
//
LOCUS       DAOSIO010000207         2543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_35406_length_2543_cov_3.418006, whole genome shotgun sequence.
ACCESSION   DAOSIO010000207 DAOSIO010000000
VERSION     DAOSIO010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35406_length_2543_cov_3.418006"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /gene="rodA"
                     /locus_tag="PK590_07160"
     CDS             <1..1076
                     /gene="rodA"
                     /locus_tag="PK590_07160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOG24412.1"
                     /translation="KVLKDLHWPRSVAVMSLAVIGILFIYSASFRDSGQYEIKQVLWA
                     VIGLAIMLAIPYVGYRPLLSVGYLFYGFSVILLILVFFVGSKHFGAQRWLNVGPLLLQ
                     PSEFAKLGTLLALVNYLGSRNPLQGQTKTVLGALVLLAVPWLLVVKQPDLGTALLFIP
                     MVLVMFFLWGIRWRFLITAFAGVLVMAPLAWEMLKDYQKKRILVFLNPNLDPLGSGYT
                     AIQSKIAVGSGGLMGKGYLAGTQTQLHFVPEHHTDFIFCVIGEELGFIGTLLVLCLYA
                     YLFQAIFLIMERTTDVKAKLLAGGVLALLASHVLINVGMTIGLMPITGLPLPLISYGG
                     SSFIMNCIALGLVLSVYKERSIF"
     gene            1311..>2543
                     /locus_tag="PK590_07165"
     CDS             1311..>2543
                     /locus_tag="PK590_07165"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOG24413.1"
                     /translation="MFKVFQKNEEGARGVYGFPHREFERIVRFEEDEPFEILGPHPSS
                     GKMVQVRSFLPRAVDAWIRLPGSGMRRPMKKVHPKGIFEVVVKSSFPVMDYRVGFHDE
                     NGFASETEDPYAFRPEISDLDLFLIGEGNHFNSYKKFGAQLTQIRGVSGVHFSVWAPN
                     AKSVSVVGNFNHWIPGAHPMARIHFSGVWGLFIPGIDEGELYKFAIRSSADHEVRIKT
                     DPYAFRAELRPRTASIVSNLDRYQWNDEAWMQNRAKDHPLAHPISVYEIHLGSWMRDE
                     KNGWGFMDYRELAGKLVSYVKCMGYTHVELLPVMEHPLDESWGYQVVNYYAPTSRFGD
                     PEGFMYLVDLCHQHGIGVILDWVPAHFPKDSYGLVDFDGRQIYAYENWKKGEHRDWGT
                     LVFDYGKREVQNFLISNAL"
BASE COUNT          565 a          567 c          685 g          726 t
ORIGIN      
        1 gaaaagtgct taaagatctc cattggccgc gtagtgtggc ggtgatgagt ctggcagtta
       61 tcgggattct ttttatctat agcgcttctt tccgcgattc cggtcagtac gaaatcaaac
      121 aagtcttgtg ggccgtgata gggcttgcga tcatgctggc tatcccttac gtgggatatc
      181 ggcccttgct tagtgtcggg tatctatttt acggattcag tgtgattctt ttgatccttg
      241 ttttttttgt gggatccaaa cattttgggg cgcaacgctg gctcaatgtg gggcctctac
      301 ttcttcaacc ttccgagttt gcgaaacttg ggacattgct ggcgcttgtc aattatctgg
      361 gatcccgcaa tccccttcag ggacagacca agacggtttt gggcgcgctg gttttgctgg
      421 cggtaccgtg gcttttggtc gtcaaacaac cggatcttgg gacagccctg ctttttatcc
      481 cgatggtact cgtcatgttt tttttgtggg gtatccggtg gcggtttttg atcacggcct
      541 tcgcgggggt tctggtgatg gcgcctcttg cctgggaaat gctcaaggat tatcagaaaa
      601 agagaattct ggtttttctc aatccaaatc tggatccgct cggttccggg tacactgcca
      661 ttcagtcgaa aattgcggtg ggctcaggag gattgatggg gaaggggtat cttgccggta
      721 cccaaaccca gctacacttt gttccggaac accataccga tttcattttt tgtgtgatcg
      781 gggaagagct cggttttatc gggaccttgc ttgtcctttg tctttacgcg tacctttttc
      841 aggccatctt cctcatcatg gagcgcacga ccgacgtcaa ggcgaaactg ctggcgggcg
      901 gggtgttagc ccttcttgcc tcccacgttc tgatcaatgt cggaatgacg atcggcctca
      961 tgccaatcac gggcctcccc cttccgctga ttagctacgg aggatcttcg tttattatga
     1021 actgcatagc gcttggctta gtgttaagtg tctataagga aaggtcgatt ttttaggctg
     1081 tttttctgtc ttgggcggtt ccatagtaac aagctccgaa aaattttgaa aataaggtac
     1141 aatacgcata tccaatacca acaaaaataa aatttaacat actactcgcc aagtttgaga
     1201 agcggcttcc ctcttacaaa agaggggaag ggaaaggtta atgaaaggca tgccaagaga
     1261 acgattttat gttggctgta tcactcacac caatgaattg acggaacctt atgtttaaag
     1321 tatttcaaaa aaatgaagag ggtgcgcgag gcgtctacgg atttccccac cgcgaatttg
     1381 agcggattgt ccggtttgag gaagatgaac cgtttgagat tttaggtccc cacccgtcgt
     1441 cagggaaaat ggttcaggtg aggtcgtttt tgcctcgagc tgtggatgcc tggattcgat
     1501 tgccgggctc gggcatgaga aggccaatga aaaaagtgca tccaaaggga atcttcgaag
     1561 tcgtggtgaa atcttctttt cccgttatgg actatcgggt gggattccat gatgagaacg
     1621 gttttgccag cgaaacggaa gatccctacg cgtttcgtcc cgaaatcagc gatttagatc
     1681 ttttcttgat cggagagggg aatcacttca acagttataa aaagttcgga gcccaactca
     1741 ctcagattcg cggagtgtcc ggagtacact tttcagtctg ggcgccgaat gccaaaagcg
     1801 tcagtgttgt gggcaatttt aatcattgga tccccggagc tcatcccatg gcccgcattc
     1861 atttttccgg ggtttggggg ctttttatcc cgggaataga tgagggtgag ctgtataagt
     1921 ttgccatccg ttcatcggcg gatcatgaag tccggattaa aaccgatccg tacgcgttcc
     1981 gggccgaact tcggccgcgt accgcgtcga tcgtgtccaa cctggatcgg tatcaatgga
     2041 acgatgaagc ctggatgcaa aatcgtgcca aggaccatcc cctggctcat cccatttcgg
     2101 tttatgagat tcatctgggt tcctggatgc gtgacgagaa aaacggatgg ggctttatgg
     2161 actatcgtga gttggccgga aagcttgtca gctatgtcaa atgcatgggc tacactcacg
     2221 ttgaattgct tccggtcatg gaacatcccc ttgatgagtc ctggggttat caagtggtca
     2281 actactatgc tccgaccagc cgatttggtg atcccgaagg ttttatgtat ctggtggatt
     2341 tgtgtcatca acatgggatt ggcgtgatcc tggattgggt ccctgcccat tttcccaaag
     2401 atagttacgg gcttgtcgat tttgacggcc ggcaaattta cgcctacgag aactggaaaa
     2461 agggcgagca tcgtgattgg gggactctgg tttttgatta cggaaagcgg gaggtgcaaa
     2521 actttctgat ttccaatgcg ctt
//
LOCUS       DAOSIO010000208         2534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_35572_length_2534_cov_5.137152, whole genome shotgun sequence.
ACCESSION   DAOSIO010000208 DAOSIO010000000
VERSION     DAOSIO010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35572_length_2534_cov_5.137152"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..>2534
                     /locus_tag="PK590_07170"
     CDS             177..>2534
                     /locus_tag="PK590_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24414.1"
                     /translation="MRTVALAVVCLFMFTTIAESAPILHSGYGMGGTEIAIDSALEKM
                     SIPSEYGTVKEIHIARRFSSQDKARRDLAPAPVVIQIEDAHSQRDAQKNINNLTRYLS
                     ETYGIKTIFVEGGMGELEPDLLRFFHAPDLNAQLLERLQDKGLIGGLEKSLSAEDGKS
                     GTESFVWPGSHQRSSAIRAFGVEDLVLYRKNLDQFRAVYAAKPETDLFLEKTRRDIVS
                     RGHRIFGKELKDYFSTWMFQNETDAKITERLSTLQKYAADVLDLDFDDAREQYDWPQL
                     VRFFKIKTLEKKGDGAERERVRERKNLVSWLEQNGLGRYVDDFQFLVSGPYAPNSKLA
                     GDVRSQVEQFYELAVSKGFRFGDYPHLTCEIGIAILSSELQATDLLGEAERLNDLILQ
                     SLAQTEEEKDLILEYKEYRLLKKLFALELTREEYQRLKNKTGGTGDGEKELKWFSGSF
                     RGEPADRSKLPVAKAMSFYEIAEAREQVMFENIRARMEELQTTRSILITGGYHSEGLA
                     EFFRQGGISYVRIAPRIAEINSDNSYHHAMMLGQSGIGALTDAVSSRAEGEKSGTATS
                     GTALRFATLAVKPELFERAVGRPAVLAVRQELRDSLREVFRKAGKSFSELNVLSSKAL
                     AAHGLASSSSSSGPVAPETRIAALARSEFRDSVWTDIEFPERVSFVSEGPSTAIGFPP
                     EADRFLKGEEVLGLFFDPASALMALVPYTRTLALKRFGVPFLEPQSLGRVANNLWVSI
                     LDIDLEKSGLKKFSTFGIASFGTHWALFDREHHPFLEPLGKLRFSN"
BASE COUNT          622 a          582 c          693 g          637 t
ORIGIN      
        1 tttcaaaggc cattgctaag taaaggttca ggtgttaagc tctagccacg ttcaactcat
       61 aatccggaaa agacttacga tggattccag gtctttgttt ttaacggaaa agaagggatg
      121 ccaggggtga tttgatcatc atgtttctac gggcgaacgg aggagtgttt atatttatga
      181 gaacggttgc attggcagtc gtgtgcctct ttatgttcac gacaatcgcc gagtccgcgc
      241 cgatccttca ttccgggtat ggaatgggcg gtactgaaat agccatcgac tccgcattgg
      301 aaaaaatgtc gataccttcc gaatatggaa ccgttaaaga gattcacatt gcccggcggt
      361 tttcttccca agacaaggcc cgccgcgatc tggcgccagc gcccgtggtg atccagatcg
      421 aagacgctca ctcccagcgt gatgcccaaa aaaatattaa taatctgacc cgttatcttt
      481 cggagacata cgggatcaag acgatttttg tcgaaggtgg tatgggcgag ctcgaaccgg
      541 atcttctccg gttttttcac gctccggacc tcaacgctca actgttggaa cgactgcagg
      601 acaaaggctt gatcggagga ttggagaagt ctctctcggc ggaggatgga aaatcgggaa
      661 ccgaatcttt cgtatggccc ggaagccacc agcggtcttc cgccataagg gcttttggcg
      721 ttgaagatct cgtgctttat cgcaaaaatc ttgaccagtt tcgcgctgtt tatgccgcca
      781 aacctgaaac tgatttgttc cttgaaaaaa cgcgacggga cattgtgtcg cgtgggcacc
      841 ggatttttgg taaagagctg aaagactatt tttcaacatg gatgtttcaa aacgaaaccg
      901 acgcgaaaat tacagaacgc ctttccacgc tccagaaata cgcggctgac gtccttgatc
      961 tggattttga tgacgctcgt gagcagtatg actggcccca gctggttcga tttttcaaaa
     1021 tcaaaacact tgaaaaaaaa ggagacgggg cggaacggga aagagtaagg gaaagaaaaa
     1081 atctcgtgag ttggctggaa caaaatggat tggggcgcta cgtggatgat tttcagtttt
     1141 tggtttctgg accctatgcc ccgaactcta aactcgcggg tgacgttcga tctcaagtag
     1201 aacagttcta tgaactggcg gtttccaagg gcttccggtt cggagattat cctcatctta
     1261 cctgtgaaat cggaattgcc attctctcca gtgaattaca agcgaccgat cttttggggg
     1321 aagcggagag gttgaatgac ctgattttgc aaagccttgc ccagacggaa gaggaaaagg
     1381 atctcattct ggagtacaag gaatatcgcc tattgaagaa actatttgcc cttgaattga
     1441 cacgagagga atatcagagg cttaaaaata aaacaggagg aaccggggat ggagaaaaag
     1501 agctgaaatg gttcagcggt tctttccgcg gggagccggc tgaccgttca aaattgcctg
     1561 tcgcgaaagc gatgagtttt tatgaaatcg ccgaagctcg cgaacaagtt atgtttgaaa
     1621 atatacgggc gcgcatggag gagttacaaa cgacgaggtc aatcctcatc acgggagggt
     1681 atcattccga gggcctcgcg gagtttttta gacagggtgg aatttcttat gtccgcatcg
     1741 cgccgcgcat cgcggaaatc aattcggata atagctatca tcacgcgatg atgctgggcc
     1801 agtccgggat cggcgctctg accgacgcgg tatcctctcg ggccgaaggt gaaaaatccg
     1861 ggacggctac aagcggtacg gctcttcgat tcgcgacgtt ggccgttaaa cccgaattat
     1921 ttgaacgagc cgtgggccgg ccagccgttc tcgctgtccg gcaggaactc cgcgattccc
     1981 ttcgcgaggt atttcgtaag gctggaaagt ctttcagtga gttgaacgta ctttcctcca
     2041 aggcgctagc cgcccacggg cttgcttctt cgtcctcctc tagcgggcct gtggcgcctg
     2101 agacaagaat cgcggccctg gcaaggtcag agttccgtga cagcgtttgg acggacatcg
     2161 aatttcccga gagggtctcg tttgtttcgg aagggccttc aactgcgata ggatttcccc
     2221 cggaagctga tcgttttttg aagggagagg aggttctcgg actctttttt gacccggcaa
     2281 gtgctttgat ggccctggtg ccctacaccc ggactttggc gctcaagcga tttggcgttc
     2341 cttttctgga gccgcagtca ttaggcaggg tggcaaataa tctctgggtt tccattctgg
     2401 acattgatct cgaaaaatca gggcttaaaa aattttcgac ttttggaata gcgagcttcg
     2461 ggacacactg ggcgctgttt gatcgtgaac atcatccgtt tttagagccc ctggggaagt
     2521 tgaggttttc taac
//
LOCUS       DAOSIO010000209         2527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_35699_length_2527_cov_3.893608, whole genome shotgun sequence.
ACCESSION   DAOSIO010000209 DAOSIO010000000
VERSION     DAOSIO010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35699_length_2527_cov_3.893608"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /gene="fusA"
                     /locus_tag="PK590_07175"
     CDS             complement(<1..1354)
                     /gene="fusA"
                     /locus_tag="PK590_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOG24415.1"
                     /translation="MADDKKIHYPLEKIRNIGIAAHIDAGKTTTSERILFYTGRTYKI
                     GEVHEGTAVMDWMEQEQERGITITSAATTCFWKDCRINLIDTPGHVDFTIEVERSLRV
                     LDGCVAVFGAVEGVEPQSETVWRQADRYHVPRIAYINKMDRTGAEFEKNIQQMRDQLG
                     ANAIPIQYPIGREENFKGVIDLIEMKAFVFEGDSLEAKFQTIDIPEEYLDVCKKAREF
                     MIEKVSECDDHLMQKFIEGQEPTAAEIKAAIRKGTISLKMTPVIVGSSLKNKGIQQML
                     DAIVDYLPSPKDVPPVKGTDPETGNEIVRETDDKAPLSCYAFKIASDKFVGTLTFIRI
                     YSGTLSSASYITNTRTGKTERLGRLLIMHSNKREEIDEAGAGNIVAAVGLKDVKTGDT
                     FCDGKHPILLEAMFVPEPVIWMAIEPKTKADRDKLADAIGKLVAEDPTFRVKTDQETV
                     Q"
     gene            complement(1424..1891)
                     /gene="rpsG"
                     /locus_tag="PK590_07180"
     CDS             complement(1424..1891)
                     /gene="rpsG"
                     /locus_tag="PK590_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOG24416.1"
                     /translation="MRRRRAQKRRIDPDPRFNDLLVAKLINIVMERGKKSIAEKIVYQ
                     AFDIIKEKTSKEPLEVFHHSVDNIRPLLETKSRRVGGATYQVPVSVPEERGQSLALRW
                     IRAYSRERKGKPMHEKLAQELIDGFNKTGAAVKKREDVHKMAESNRAFAHYRW"
     gene            complement(1888..2280)
                     /gene="rpsL"
                     /locus_tag="PK590_07185"
     CDS             complement(1888..2280)
                     /gene="rpsL"
                     /locus_tag="PK590_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOG24417.1"
                     /translation="MAPTINQLIKYGRKKFETKSKSPALHNAPFRKGVCLMVRTQTPK
                     KPNSALRKIARVRLTNGEEVTAYIPGVGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGK
                     LDTSGVADRTKSRSKYGAKTPKKDAKAA"
BASE COUNT          565 a          675 c          544 g          743 t
ORIGIN      
        1 tttgcaccgt ttcctggtct gtcttgacgc ggaacgtcgg atcttcagca acgagctttc
       61 caatcgcgtc cgcgagctta tcacgatccg ctttggtctt cggctcaatc gccatccaga
      121 tgacaggctc cgggacaaac atcgcctcaa gaagtatagg atgcttgcca tcgcaaaaag
      181 tatcaccggt ttttacatcc ttaagcccga ccgccgcaac gatgtttcca gcgccagcct
      241 catcaatttc ttcacgtttg ttcgaatgca taatcaaaag tcgtcccaaa cgctcagttt
      301 ttccggtccg agtgttggtg atataggatg ccgacgagag cgtgccagaa taaattcgaa
      361 taaaggtcag agtacccaca aacttatcgc ttgcgatctt gaacgcgtaa caactgagcg
      421 gcgctttatc atccgtctca cggacaattt catttccggt ttcgggatct gtacctttga
      481 ccggcggcac atccttcggt gaaggcaaat agtccacgat cgcatcaagc atttgttgta
      541 tgcctttgtt ctttaatgaa gagcccacaa ttaccggcgt catcttgagt gagatcgttc
      601 ctttgcgaat cgcggccttt atttccgcgg ctgtcggctc ctgaccttca ataaattttt
      661 gcatcaaatg gtcatcacat tcagagacct tctctatcat aaattcgcgg gcttttttgc
      721 aaacgtcaag atactcttcg ggaatatcga tggtttgaaa tttagcttca aggctgtcac
      781 cctcaaaaac aaaagccttc atttcaataa gatcaatcac ccctttgaag ttttcttctc
      841 gaccgatcgg atactgtatc ggaatggcgt tcgccccgag ctgatcacgc atttgctgga
      901 tatttttttc gaattccgcg ccggtacgat ccatcttgtt gatgtaggca atccggggaa
      961 catgataccg atccgcctga cgccaaaccg tttcggactg tggttcgaca ccttccacag
     1021 cgccaaaaac cgcgacacaa ccgtccagca cgcgcaaaga cctttcgacc tcaatggtaa
     1081 aatcgacgtg tccgggggta tcaataagat taatccggca atccttccaa aagcaggtcg
     1141 tcgccgcgga tgtgatggta ataccgcgct cctgctcttg ttccatccag tccatcaccg
     1201 ccgtcccttc atgcacctca ccaatcttat aggtgcgccc ggtgtaaaaa agaatgcgct
     1261 cagacgtagt ggtttttccc gcgtcgatat gcgccgcaat accgatgttg cgaatttttt
     1321 ctagcggata atgtattttt ttatcgtctg ccatgatatt tcttttctat tcaattgtgt
     1381 taccgcgtaa attttggagc cgccaatcgt ttccggccgt ctattaccaa cgatagtgcg
     1441 cgaacgccct gttggactca gccatcttgt gaacatcttc tctcttttta acggccgcac
     1501 ctgttttatt aaagccatca atcagttcct gcgcgagttt ttcgtgcatg ggctttccct
     1561 ttcgttcacg ggaataggca cggatccatc gaagcgcaag agattggcca cgctcttccg
     1621 ggacactgac cggcacctgg taggtcgccc cgccaacccg gcgagacttc gtttccaaaa
     1681 gaggccggat attatccacg gaatgatgaa aaacttcaag aggttctttt gaagtttttt
     1741 ctttgataat atcaaacgcc tggtaaacaa ttttttcggc gattgatttt ttcccgcgct
     1801 ccatgacgat attgatcagc tttgcgacca ataaatcatt aaaccgcggg tcggggtcaa
     1861 ttcttctttt ttgtgctcga cgccttctca tgctgctttt gcatccttct tgggtgtttt
     1921 ggctccgtat ttggagcgtg atttagtgcg atccgcgaca ccgctggtgt cgagttttcc
     1981 gcgaacaata tgatagcgta cgccggggag atccttaacg cgtcctccgc gcacaagtac
     2041 aatcgagtgc tcctgaagat tatgtcccac ccccgggata taggccgtga cttcctctcc
     2101 attagtaagc ctcaccctcg cgatctttcg tagcgccgag tttggcttct tgggagtttg
     2161 tgttcgaacc ataagacaaa cgccttttcg aaacggcgcg ttatgcaaag ccggagattt
     2221 cgactttgtc tcaaattttt ttctaccgta cttgatcaac tgatttattg taggtgccat
     2281 tatcttttat cccgtttaaa aaggttcctg acctatctgt tctgtttaat gttttattcg
     2341 caactttact taacccgtga gcctaccctc cggctcttca acggagtgcg tctttcgacg
     2401 aaacttcttc cgtcgccttt gccttgggtt tttccggatc cggcaacacg tccgtcagat
     2461 ctttttccat ttcaatatta tggtgcattt taaaacccgt ccccgcaggt acgagatgcc
     2521 ccataat
//
LOCUS       DAOSIO010000210         2502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_36165_length_2502_cov_3.796077, whole genome shotgun sequence.
ACCESSION   DAOSIO010000210 DAOSIO010000000
VERSION     DAOSIO010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36165_length_2502_cov_3.796077"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="PK590_07190"
     CDS             complement(<1..870)
                     /locus_tag="PK590_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOG24418.1"
                     /translation="MAKLVSASSKTRQLLLLSISAALFLMVIKFITGLFTHSMSIMAS
                     ALDSALDAAMSFVNLVAFHEASKPPDEEHAYGHGKIESLASLFQSLLILLSGSYVIYE
                     AVKRLSFGSYVTVIPIGILVIVFSGGVTFLLAWKLRRMSRETKSLILRTETLHYTMDF
                     VAYAGVLLALILVSITGWVIWDLVIAMVMVGYIFWEALKILKRAGGDLLDRGLSKKEL
                     AELRKLILNHHRSIVGVHNLRSRFSGNQLFLDFHIVIRGEGDFVRAHEMTESLIAKIK
                     EQRPQSDITVHFDP"
     gene            complement(863..1393)
                     /locus_tag="PK590_07195"
     CDS             complement(863..1393)
                     /locus_tag="PK590_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOG24419.1"
                     /translation="MITIKQGDITKESVDVIVNAANTGLKGGGGVDGAIHRAAGPQVM
                     VECRAIGHCPTGTAVITGAGNLLAKKIIHTAGPVWNGGSRREPELLRDCYVASFRLAK
                     AHGLRTIAFPAISTGVYGYPKREAATIALSVGIQFEKNFDEIRYLCFTTEDLQIYQDT
                     YATLRKQPGADHTCNG"
     gene            complement(1572..>2502)
                     /locus_tag="PK590_07200"
     CDS             complement(1572..>2502)
                     /locus_tag="PK590_07200"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24420.1"
                     /translation="NQFPAAILFLDVDPRNVDVNVHPTKQQVRLSHEGMIKSLVEKTV
                     SQALNQNQDPLPRSGVAGEMSLGEPPSQPISHTSDLSFGNSESGASFKVAEGSNDEDS
                     QPSAVFPIFIGKEIQITKILGQIHRTYIVAETEEGFVLVDQHAAHERVMFEILMKNFQ
                     SQTPARQKLLIEETFDLHPRQVETMRKYLEFLNQIGFETEWFGERSFVVRGVPSILAD
                     VNPVTILKSFVEEKEGGKVRTSLKGAEEDVAAIVACKRRSVKASDPLNLEEMRGLLKS
                     LGQCQNPFHCPHGRPTLIRHATGDIEKQFGRRG"
BASE COUNT          676 a          692 c          514 g          620 t
ORIGIN      
        1 aggatcaaaa tgaaccgtaa tgtctgattg gggccgttgt tccttaattt ttgcgatcag
       61 cgactccgtc atttcatggg ctcggacaaa atccccttct ccccgaatga cgatatgaaa
      121 atcaagaaaa agctggttgc ccgaaaatcg gctcctaaga ttgtgtactc ccacaattga
      181 acgatggtga ttcaagatca attttcttaa ctcggccaat tcctttttgg acagaccacg
      241 atcaagaaga tcaccccccg cgcgcttcag tatttttaaa gcttcccaaa agatataccc
      301 taccataacc atggcaatca caagatccca aatgacccat cccgtaatcg acacaaggat
      361 cagggccagc aaaacccccg cgtacgccac gaaatccatc gtgtaatgca acgtctccgt
      421 tcttaaaatt aatgacttgg tttcgcggct catcctccgg agcttccagg caagtaaaaa
      481 cgtaactcct cccgaaaaaa caatcacgag aatcccgatg ggaattacgg tgacataact
      541 gccaaaactc aaccttttca cggcttcgta gataacataa gatccgctaa gcaaaatcag
      601 cagactctga aaaaggctgg caagactttc aatttttcca tgaccgtagg cgtgttcttc
      661 atccggcggt ttagaggctt catgaaacgc aactaaattg acgaaagaca tcgccgcatc
      721 aagcgctgaa tctagcgcgg atgccataat ggacatcgag tgcgtgaaca gacccgtgat
      781 aaatttaatc accatcagga ataaggccgc tgaaattgaa aggagaagta attgccgagt
      841 ctttgacgaa gcagaaacta atttagccat tgcaggtatg gtccgctcca ggttgtttac
      901 gaagagttgc gtaagtatcc tgatagatct gaagatcttc ggtcgtgaaa cagagatacc
      961 gaatttcgtc aaaatttttt tcaaactgaa tcccgacgct caaggcaatc gtcgccgcct
     1021 cacgcttcgg atagccatac acccccgtac taatcgcagg gaaagcaatc gttcggagcc
     1081 cgtgagcctt cgctaatcta aaactggcca cataacagtc cctcaggagc tccggttccc
     1141 gtcgacttcc cccgttccag acaggccctg ccgtatggat aatcttcttt gccagcaaat
     1201 ttccggctcc cgtaatcacc gccgttccgg tcgggcaatg tccaatggca cggcattcca
     1261 ccatcacctg aggtcccgcc gcgcgatgaa tggccccatc aacgccaccg cctcctttca
     1321 atcccgtgtt agccgcattg acgatgacat ccacgctttc cttcgtgatg tccccctgtt
     1381 tgatcgtgat cacaatccct gtctcctatt tcctgcatcc agcaaataaa tcaagatatt
     1441 gcgccaaaaa cccttcatga gaaacttctt ctttacgttc tcatgcaacc gccgtcccat
     1501 ctgatcccgc aatttccgat ctttgactag ctgaacgata tagtgcgcgg cttggccact
     1561 atgtttgaga attatcccct tctgccgaat tgtttttcaa tatcgccagt cgcatgccgg
     1621 atcagtgtgg gacgaccgtg aggacaatga aacgggtttt ggcattgccc cagactcttc
     1681 aaaagacccc gcatctcctc aaggttcaga ggatccgacg ccttaacaga acgacgttta
     1741 cacgcaacga tcgccgcgac atcctcttcg gcgcccttca gcgatgtccg aacctttccg
     1801 ccttcttttt cttcaacaaa gctttttaaa atcgtaacgg gatttacatc ggccagaatc
     1861 gacggaaccc cgcgaacaac aaacgaccgc tctccaaacc attccgtttc aaagccaatt
     1921 tgatttaaga actccagata ctttctcatc gtctcaacct gccggggatg aagatcaaat
     1981 gtttcttcaa tcaaaagctt ctggcgagcg ggagtttggg actgaaaatt cttcatcaat
     2041 atttcaaaca taacccgctc gtgcgccgca tgttgatcca ctagaacaaa accctcttcc
     2101 gtttcagcca caatgtaagt gcgatgaatt tgaccaagaa tttttgtaat ctggatttct
     2161 tttccaataa aaatggggaa aacggccgat ggctgggaat cctcgtcatt cgatccttcc
     2221 gccaccttga atgaagcccc gctttccgaa tttccgaacg acaaatcact ggtatgcgaa
     2281 atcggctgag atggtggttc accaagagac atttctccgg caacgcccga ccgcggcaaa
     2341 ggatcctgat tctggttaag cgcctgagaa actgtttttt caactagaga tttaatcatc
     2401 ccctcgtggg aaagccgcac ctgctgtttt gtcggatgca cgttgacatc cacgtttcgg
     2461 ggatccacat ccagaaaaag aatcgccgcg ggaaactgat tt
//
LOCUS       DAOSIO010000211         2491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_36382_length_2491_cov_3.569376, whole genome shotgun sequence.
ACCESSION   DAOSIO010000211 DAOSIO010000000
VERSION     DAOSIO010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36382_length_2491_cov_3.569376"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..>814
                     /locus_tag="PK590_07205"
     CDS             103..>814
                     /locus_tag="PK590_07205"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HOG24421.1"
                     /translation="MDSSKPPLRRSLSAKFLITAILALAFGLRLWSVGFGLPHLYHAD
                     EPIVVNHALAYGAGDLHPHFFNIPPLVSYLLFVGYGLYYCAGAVTGIFPALRDFEYLF
                     YGNPSSFYLIARVIFGVILGAFSVFLLYRLSRRWGSEREALLSALFLAVNFLHARDSH
                     YIYVDIPLVFVLLGGFILMFRLFEPVSETREKEERRFTGWGRHFVVGAMMGLATAVKY
                     NGVFLLIPYVWISIRLLSP"
     assembly_gap    815..824
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <825..1868
                     /locus_tag="PK590_07210"
     CDS             <825..1868
                     /locus_tag="PK590_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24422.1"
                     /translation="RQWIRAWLVAGCAAFLVFVILNPFSALDVDFFLSELREQSAANQ
                     GNFSMFHHFRYSLMGSFTFVALLLALAGVVKGLFSRDVRRQAISVFIFGYYAVLCRWG
                     QPYDRYVLPLVPFLCLTLSDMILTMNRVWGRKMRGFTFPQINHKTRVMITSLLVALCL
                     LPPLLKIVCWNVLMSKPDTRTLAREWIERNIPSGAKVALDREFHGPRLRFTQDQLREK
                     LPNATPAQSRRVQALIEWPREPSYELYFTAVDLTASRFLFAQPVVLRDLEEVKRKGIR
                     WIVTTSAASPPDNFLLELENRAVLSASFSPFRDGRNDAIHDEQILTGGPFLWRDILAR
                     ERNGYPILIYELR"
     gene            complement(1849..>2491)
                     /locus_tag="PK590_07215"
     CDS             complement(1849..>2491)
                     /locus_tag="PK590_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG24423.1"
                     /translation="IEDGSRDNTLAVLRNEVVKYPNTRLIIHEKNMGYNASLRDGLRA
                     SRGQYMMYIGADEEFDSSEIPQFVEILRKGEADVLLGVRWQRNAYQLFRFFLSVIYIF
                     FLNWLYKVRINDYNWSQAWNRKVFEKIELTSHSLFVLPEIIVKAIDLGFKIKEIPSNH
                     RGRQWGKSSLNMRIMGAALWDSIQFGWLRKTSKYRPESSHPFLSRSQSIEVHR"
BASE COUNT          479 a          590 c          687 g          725 t
ORIGIN      
        1 caagatccgt gaagagcctg tgatttggcg caattccagc caaagccggg tccagttgat
       61 cagagatcca atcgccatgt tctttgatct catcaaaatc agatggattc atcgaaacct
      121 ccgctaagga ggtccctgtc ggcgaagttt ctgataaccg cgattttagc tctcgccttt
      181 gggctccgac tctggagcgt tgggttcgga cttccacacc tttatcacgc ggacgagccg
      241 attgtggtca atcacgcgct ggcctacggc gcaggcgatc ttcatcctca tttttttaat
      301 attcctccct tggtgagtta cctgttgttt gtcggttacg gtctctatta ttgcgcgggg
      361 gcggtgacgg ggatttttcc ggcgctccgg gattttgagt atttgttcta tggaaacccc
      421 tcgtcttttt acctcattgc ccgggtgatt ttcggggtga ttctcggcgc gttttccgtc
      481 ttcctcctgt atcgtttgag ccggcggtgg ggaagtgaaa gggaagcctt gctttctgca
      541 ttgttccttg ccgtcaattt tcttcacgcg cgagattcgc attatattta cgtggatatt
      601 ccgctggtgt tcgttctttt gggtggtttt attctgatgt tccggttgtt tgaaccggtt
      661 tcggaaaccc gtgagaaaga ggaacggcga tttacgggat ggggccgaca ttttgtggtt
      721 ggcgctatga tgggtctcgc gacggcggtc aaatataacg gtgtttttct tttgatacct
      781 tacgtctgga tttcgatcag gctactgtct cctcnnnnnn nnnncgacag tggatccggg
      841 catggctggt ggccggatgt gccgcctttc tggtttttgt gattttgaac ccgttctcgg
      901 cccttgatgt cgattttttt ctcagtgagc ttcgagaaca gtcggccgcc aatcagggaa
      961 acttttctat gttccatcat tttcggtatt cgctgatggg gtcttttacg tttgttgctt
     1021 tgctgttggc gttggcgggt gtcgtcaaag gtcttttctc ccgggatgtc cggcgccagg
     1081 cgatttctgt tttcattttt ggatattatg cggtgctttg ccgatgggga caaccttatg
     1141 accggtacgt tctgccgctt gtgccattct tatgcctgac tttatcggat atgattttaa
     1201 cgatgaatcg tgtctggggc cgaaaaatga gaggctttac atttccccaa ataaaccata
     1261 agacgcgcgt catgatcacg tcgttactgg tggcgctgtg tctccttcct ccgcttctga
     1321 agatcgtgtg ctggaatgtg ctgatgtcga aacccgatac gcgaacgctg gcgcgggagt
     1381 ggatcgaacg gaatattcct tccggagcga aggtcgcgct tgaccgcgag tttcacggac
     1441 cccggttgcg tttcacgcaa gatcagttgc gtgaaaaact cccgaacgcg actcctgcgc
     1501 aatcccggcg ggtccaggct ctgattgaat ggccgcggga gccttcttat gagctttatt
     1561 ttacggcggt ggatctgacg gcgtcccggt ttttgttcgc gcagccggtt gtgcttcgtg
     1621 atctggagga ggtaaaacgg aaggggatac gctggatcgt tacgacgtct gccgccagtc
     1681 cgccggataa tttcctgctt gaacttgaga accgagctgt gctgtcggca agttttagcc
     1741 ctttcaggga cggacgtaac gatgccattc atgatgagca gattttgaca ggcggtccct
     1801 ttttgtggag agatattttg gcgagggaga gaaacggtta tccgattctt atctatgaac
     1861 ttcgatagat tgagagcggg aaagaaaggg atggctcgat tcgggacgat attttgaagt
     1921 ttttcggagc catccaaact gaatgctgtc ccagagcgcc gcgcccatga tcctcatatt
     1981 cagcgaagat tttccccatt gacggccccg gtggttcgag gggatttctt tgatcttgaa
     2041 ccccaggtcg attgccttga cgataatttc cggcaataca aacagggaat gggaagtcag
     2101 ctcaatcttt tcaaagacct tccggttcca ggcttgcgac cagttgtaat cgttgatcct
     2161 taccttgtaa agccagttga gaaaaaagat atagatgacg gaaaggaaga acctgaaaag
     2221 ctgatacgcg ttcctttgcc accggacgcc gagaagaacg tccgcctcgc ctttacggag
     2281 aatctcgaca aactggggga tttcgcttga atcgaattcc tcgtccgcgc cgatgtacat
     2341 catgtattgg ccccgggatg cccgaagccc gtccctgagg gacgcgttat agcccatgtt
     2401 tttttcatgg atgatcaggc gggtgttggg gtatttcacg acctcgtttc tgaggacggc
     2461 gagcgtattg tcccgggatc cgtcctcaat c
//
LOCUS       DAOSIO010000212         2448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_37187_length_2448_cov_4.285834, whole genome shotgun sequence.
ACCESSION   DAOSIO010000212 DAOSIO010000000
VERSION     DAOSIO010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37187_length_2448_cov_4.285834"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="PK590_07220"
     CDS             complement(<1..1131)
                     /locus_tag="PK590_07220"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HOG24424.1"
                     /translation="MTISSPQSKNTEAVEARLSAEALAARGGHHGLIWMVLVAGLMIS
                     VASAFLLKTMVQASQRKDFLARCEEVRTAIADRLDDHVRILRGAAALFHASSQVSRQE
                     WHLFIEKQSIGTQLPGTQAIGFALWIPREELSRHIQTVRDEGFPDYTVHPAGERDVYT
                     SIVYLEPFSGRNLVAFGYDMFSDPVRRAAMERARDGDVAALSRRVTLIQEIDEDVQPG
                     TVMYVPVYRSGVPLDSVEQRRNALMGWIYSPYRMKDLMQGILGKRLWEKRWSLRLQLF
                     DGPEASPESLLFEDGPLSETSSLPGATFFHEMPIDFRGNVWTLRFEKSDIGFMSKEYA
                     IARFVLVAGTLVTVLLVFLIFSLSRTRTEAIRIARKLTSHLSA"
     gene            complement(1183..>2448)
                     /locus_tag="PK590_07225"
     CDS             complement(1183..>2448)
                     /locus_tag="PK590_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24425.1"
                     /translation="VQFVVPVTGCYHRSHGSTESRFFAAGSYDKAAKFMKGIFMKTGR
                     AQKGFVLALSAILLLCISVILAAFVFLTTLRSRSLAIGVASVKAFWLAEAGIQRVASQ
                     LKLDLDYRSHPVDLNGRLGEGSYTVSVATPADSGVCVATSTGTVDGISRTITQTLTIR
                     AAGWGSQFTEYAVFAGVKNSNVTLRNNAEIQGDVYVGGTVRTMDSSSVSGTVFASSGS
                     GNYVREPLPTPPVPIPTLTKKWYDDAISVAKTYPKGNVTYTSIDLTGQTLYVNGKVRL
                     TNASGTGTIVSVGSLEVNGTIGSGVSVISDSTVSIGANSRVSPDVMFYGRNAVTVDSP
                     GIVMEQVAIFSPGKVDIANGAQISGVVFSDGNITVSGNSVVAGSVAAGGNMTLNASTV
                     IQDKDKLPQRVPFGVAGKEDVIALSDWRE"
BASE COUNT          632 a          726 c          557 g          533 t
ORIGIN      
        1 cgcgcttaaa tggctggtca gctttcgtgc gatgcggatc gcctcggtac gcgtacggga
       61 taaagaaaaa atcaaaaata caagcaaaac cgtgaccagt gtcccggcga ccagaacgaa
      121 ccgcgcgatg gcgtattctt ttgacatgaa gccaatatca gatttttcaa aacggagagt
      181 ccagacattt cccctgaaat caatcggcat ttcatggaaa aaagtagcgc cgggcaaaga
      241 cgatgtttca ctcagggggc catcttcgaa aagaagcgat tccggcgaag cctcgggacc
      301 gtcaaaaagt tgtaaccgaa gagaccatct tttttcccat agacgtttcc ccagaattcc
      361 ctgcatcaaa tctttcatcc ggtaagggct atagatccat cccataagcg cgttccggcg
      421 ctgctctacg gagtccagag gtactccgct acggtaaacc ggaacataca tcacggtacc
      481 cggttgtaca tcctcgtcaa tctcctggat cagggtcact cttctggaca aggcggccac
      541 atccccgtct ctggcccgct ccatagccgc acgccggacg ggatccgaaa acatatcgta
      601 accaaaagca accaggttac gaccggaaaa aggttcaagg taaacaatgg aggtataaac
      661 atcccgctcg ccggcaggat gcacggtata gtccgggaaa ccttcgtctc tcaccgtctg
      721 aatgtgccgg gacaattctt cacgagggat ccaaagcgcg aatccaatcg cctgggttcc
      781 aggaagctgc gtcccaatgc tctgtttctc gatgaaaaga tgccactctt gacgagacac
      841 ttgagacgac gcatgaaata acgccgcagc gccccgcaaa attcggacat ggtcatcaag
      901 cctgtcggca atcgcggtcc tgacttcttc gcaacgagcg agaaaatctt ttcgctggga
      961 cgcttgcacc atcgttttta agagaaaagc cgacgcgacg gatatcatca gccctgccac
     1021 aagcaccatc cagatcaaac catggtgccc gccacgcgcg gccaaagctt cagccgacaa
     1081 gcgagcctcg acagcctcag tatttttgga ttgcggacta gaaatcgtca taccttctcc
     1141 cgattagctt tcacgtcaaa agcaatcaac atttttcaga ttttactcac gccaatccga
     1201 caacgcaatc acgtcttctt ttccagcgac cccaaacggt acccgctggg gcaatttatc
     1261 cttatcctgt atcaccgttg atgcgttaag cgtcatgttg ccacccgccg ccaccgaccc
     1321 cgctacaacg gagtttccac tcaccgtaat attgccatcg gaaaatacca ctccgctgat
     1381 ttgggctcca tttgcaatgt ctactttgcc gggcgagaaa atcgccacct gttccatcac
     1441 aatgcccggg ctatccaccg tcacggcatt tcttccgtaa aacatcacat ccggagaaac
     1501 ccggctatta gcaccgatcg aaactgtgct gtcggaaatg acagaaacac cgctaccgat
     1561 cgttccgttc acctccaggc ttcccacgct gacaatcgta cccgtcccgg aggcgttcgt
     1621 aagccttacc ttgccgttca cataaagggt ttgacccgtc agatcaatgg acgtatacgt
     1681 gacatttccc ttggggtagg ttttcgcaac tgatatcgca tcgtcatacc atttcttggt
     1741 aagtgtcggt atcggcactg ggggagtggg aagtggttcc cggacataat tcccgctacc
     1801 gcttgaggca aaaactgttc ccgaaactga ggagctatcc atcgtccgga ccgttccccc
     1861 cacatacacg tctccctgaa tttccgcatt attcctcaag gtaacattgc tgtttttcac
     1921 cccggcgaat accgcatact cggtaaactg tgatccccaa ccggcggcgc gaatcgtcaa
     1981 tgtctgggta atcgtccgcg aaattccgtc caccgttccc gtggaggtcg cgacgcacac
     2041 tcccgaatcc gccggagtag caacgctcac tgtataactt ccctcaccca gcctgccgtt
     2101 aaggtccacg gggtgggaac gataatccag atccagtttg agttgagaag cgacccgctg
     2161 aataccggct tcggcgagcc aaaacgcctt gacactagcc accccgatcg ccagtgatct
     2221 cgacctcaac gtcgtcaaaa aaacgaacgc cgccaaaata accgaaatac acaaaagaag
     2281 gatcgctgat agtgcgagca caaatccttt ttgagcccgg cccgttttca taaagattcc
     2341 tttcataaat ttcgcggcct tatcgtacga accagccgca aagaatcgcg actctgttga
     2401 gccgtgagat cgatggtaac aaccggtgac tggaacgaca aactggac
//
LOCUS       DAOSIO010000213         2430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_37541_length_2430_cov_4.293053, whole genome shotgun sequence.
ACCESSION   DAOSIO010000213 DAOSIO010000000
VERSION     DAOSIO010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37541_length_2430_cov_4.293053"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..1328
                     /locus_tag="PK590_07230"
     CDS             42..1328
                     /locus_tag="PK590_07230"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOG24426.1"
                     /translation="MSQLETHALLAFVGSSRYTNASLSFANGEFSKMKSLLKGNALIA
                     QSGGCTAVINQSLVGAAMASRASNAIPKVFGSRNGIEGILKDSLVNLSASDVELLRKI
                     AKTPSAALGSSRYKLLPEDGKKIVHHLKKHDIRYLFLIGGNDTANTSLQIAKAARLGD
                     YTLRVVHVPKTIDNDLVETDHCPGYGSVARFAAITTQEAALDTRAMKNVDPIKIIELM
                     GRNSGWIVAASALLKKSPADAPHILLIPEIPFDETAFLKRTEATLQSVGYCIIVISET
                     IRDHAGNRIGRKLGGVTKDPFGHPYVEGASPRLASILEKNLRVRARFDKPGTIQRMSM
                     PYISPVDQKEAYNAGARAVQWALGGMSEVMVGFFRKPGKKYALEYRPVPLAKIPNQER
                     YLPAEFFDAKHSMIKPAFFDYALPLIGAKLPEFPVL"
     gene            complement(1692..2210)
                     /locus_tag="PK590_07235"
     CDS             complement(1692..2210)
                     /locus_tag="PK590_07235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG24427.1"
                     /translation="MYKKNVAYLGGLQNIKNSSILPLIFKKITSLISDVQFHIIGDGR
                     LGNVVRKKCGEFNLPVVFHGNRPPHDMPELINCMDLIILPSKNEGLPVVALESLACGV
                     PMIGSDVGGIPEAIGIENVVPLDVDFIDNLSKLAVKRLTEPFEVRLDDKFKLEVVVEK
                     EYRALMQIERCA"
BASE COUNT          625 a          661 c          530 g          614 t
ORIGIN      
        1 tcaacgattc ggccaatgat cgctctatgg taagatttaa tatgagtcaa ctcgaaaccc
       61 acgcgcttct agcctttgtt ggaagttcgc gatatactaa cgcgagtctt tcgttcgcaa
      121 acggggaatt ttcaaaaatg aaatcacttc tgaaaggcaa cgcgctcatc gcgcaatcgg
      181 gcgggtgcac ggcggttatc aatcaaagcc tggtcggcgc cgcgatggct tcacgggctt
      241 cgaacgcgat ccccaaagtc ttcggctcgc ggaacgggat tgaagggatt ttgaaagatt
      301 ccctggtgaa tctctcagcg tccgatgtcg aactactcag aaaaatcgcg aagacgccgt
      361 ccgccgcgct tgggtcttcc cgttacaagc tcctgccaga ggacggaaaa aaaatcgtcc
      421 accacctaaa aaaacacgac atccgttatt tattcctcat cggcggaaac gataccgcga
      481 acacgagcct ccagatcgcg aaagcggccc gtctcgggga ctacaccctg cgagtggtac
      541 atgttcccaa aaccattgat aacgatctgg tggaaacgga ccattgtcct ggctacggct
      601 cggtcgcgcg cttcgccgcg atcaccaccc aggaggccgc cctggacacg cgcgccatga
      661 aaaacgtgga cccaatcaaa atcatcgaat tgatgggacg gaactcaggc tggattgtcg
      721 ccgcttcggc cctgctcaaa aaatcccctg ccgacgcgcc ccatatcctc ctgatccccg
      781 aaattccttt tgacgaaacc gctttcctca agcggaccga agcgactttg cagtccgtcg
      841 gttattgcat cattgtcatc tcggaaacaa ttcgcgatca cgcgggtaac cggatcggcc
      901 ggaagctggg cggagtcaca aaagatccgt ttgggcaccc ctacgtggag ggcgcctcac
      961 cccggctggc gtccattctg gaaaaaaatc ttcgggttcg cgcgcgattt gacaaacccg
     1021 gaaccattca acggatgtcg atgccctata tttccccggt tgaccaaaag gaggcttaca
     1081 acgccggcgc cagagcggtc caatgggcgc tggggggaat gtcagaagtc atggtggggt
     1141 ttttcaggaa gcccgggaaa aaatacgccc tggaataccg ccccgtgccg ctcgcaaaaa
     1201 ttccgaacca ggaacgttat ctacccgcgg aatttttcga cgcgaaacac tccatgatca
     1261 agcccgcgtt ttttgattac gccctgcccc tcatcggggc caaactcccg gaatttccag
     1321 ttctctaatc cccttctccg gcatgctact tcccgtcaaa ttgaaggttc agcattacaa
     1381 atgcaccatt tggttctctg gaatttgggg atagttcgtt gttctataca acagtcactc
     1441 gcatttcacc tcgggggtca taaccaatga ttctgccatg cacgccactc ttccttatgt
     1501 ttcgttactc tccccaagag acgcaacgaa ctgtcgatgg agccattgcg ctttatgacc
     1561 acacacccaa agcaagaggg ctgtattaat cgttggggca atcagaggtt ggggaatttc
     1621 tgaggctcaa caattgggaa gtagactcat tcttcagttt ttaccatcgg ctaatgagga
     1681 gcattacgca gttatgcaca tctctcaatt tgcattagcg ccctatattc tttttcaacc
     1741 accacctcca atttaaattt gtcgtccaac cgcacctcaa atggttctgt taaccgtttc
     1801 actgcaagtt ttgagagatt gtctataaaa tcgacatcaa gaggtacaac attctcaatc
     1861 cctatagctt cagggatccc tcccacatcg gatccaatca ttggaactcc acacgccaac
     1921 gattctaacg ccaccactgg caagccttcg tttttactcg gaagaatgat taaatccatg
     1981 cagttaatca gctcgggcat atcgtgcggg ggtctattcc cgtggaagac tacaggcagg
     2041 ttaaactcac cacatttctt tctaacaaca tttcccagac gcccatctcc aataatgtga
     2101 aattgcacat cagatattaa gctagttatt tttttaaaaa tcaaaggcaa gatggacgaa
     2161 tttttaatat tctgaagccc ccccaaatac gcaacatttt ttttgtaaat tattccgttt
     2221 ttttcttaat tcgttctatc tcatcccaag gtctctgaaa aaaaagactt gtatcaaccc
     2281 cgttatataa tactcgcttc gaaccattgt taatgttcgg ggatatatct agcaattttt
     2341 ggctgacgaa aaaattattt tttgccctaa ggataatccc cgacacgatc ttttcaatat
     2401 cattgttccg ctagcattca gtgtggatgt
//
LOCUS       DAOSIO010000214         2428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_37586_length_2428_cov_3.703751, whole genome shotgun sequence.
ACCESSION   DAOSIO010000214 DAOSIO010000000
VERSION     DAOSIO010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37586_length_2428_cov_3.703751"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PK590_07240"
     CDS             complement(<1..381)
                     /locus_tag="PK590_07240"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF024489.2"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HOG24428.1"
                     /translation="MADSVLQEKYFDRELSWIRFNARVLAEAMDPSNPPLERLKFLGI
                     VSSNFDEFFMVRVASIPETAPLFLEVYRQAFSLLRKQQLYFKEVLAPELETAGIVRVL
                     PHALSEKQQLYLKNLFLNEILPLLT"
     gene            complement(385..1950)
                     /locus_tag="PK590_07245"
     CDS             complement(385..1950)
                     /locus_tag="PK590_07245"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG24429.1"
                     /translation="MPDQEISTKHIAAVIDIGTIAIRMVIAEVGNKNEIRYLEQLQKP
                     VRFGKDVFKTGRLSNAAMRQGVNILRDFKSVIDGYGIKRVHAIATSAVREAQNRDNFI
                     DQIYVRTGIEVEILEGAEENRLELVAVESALDEKINLEKKNCLIVEVGSGSTEMILME
                     QGQVSVTRTLTIGSVRLPEQGLAAKGDEDFIQRVLKKSIRDITKNVAREVSMERIDTF
                     IALGGDVRFVSQQLLPDTVDRFTVLEKKEFVGFVSRIGKMTPEEIATQYSLDYGHAES
                     LYPALLFYLNFLNETKAEEIIIPKLSIRDGLLLEIAQLVSGYRRTDVARQVLNSTHHL
                     GAKYMYDKAHAVCVASLALKLYDLLAKDHGMGSRERLLLEVSGILHDIGIYVSPSGHH
                     KHSFYLVDAADIFGLRRTDKNIVANVVRYHRRVPPRETHVPYMSLAKSDRAIVSKLAS
                     ILRVADALDTAHQQKIRSFTLERSEDAYALWVPKDIGDISLERESLRRKGDMFADVFG
                     APIDLKQGVSPVD"
     gene            complement(1959..>2428)
                     /locus_tag="PK590_07250"
     CDS             complement(1959..>2428)
                     /locus_tag="PK590_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOG24430.1"
                     /translation="PSCSGSEISRQKVGALVGRNAIASEAGFLLLRQKCPHLLDEIGY
                     NVKKIRSGALSRAIKAGDTALEALIRSRSRWLGVALANVVNVLNPELVLLGGGMMEAM
                     GQIILPEVRSGIEAYALKPLVKVLKVVPSKLKDNAVIFGASKMACEVLKTTKK"
BASE COUNT          597 a          674 c          591 g          566 t
ORIGIN      
        1 agtgaggagc ggaaggattt cattcaggaa aagatttttc agatacagct gttgtttttc
       61 gctcaacgcg tgaggaagaa cccttacgat gccggccgtt tccagttcag gggcaagcac
      121 ttctttgaaa taaagctgtt gcttccgaag cagggaaaac gcctgccggt aaacttctaa
      181 aaaaagaggc gccgtttccg gaatgctggc caccctcacc atgaaaaatt cgtcgaaatt
      241 agaactgacg ataccgagga acttcagccg ctccagggga ggattcgaag ggtccatcgc
      301 ttccgcgaga acccgggcat tgaaccggat ccatgacagt tcccggtcaa aatatttttc
      361 ctgaagaacg ctatccgcca taggtcaatc caccggcgac acgccctgtt tcagatcgat
      421 cggcgctccg aagacatccg cgaacatatc ccctttacgg cgcaagcttt cgcgctccag
      481 agaaatatcg ccgatatctt tcggcaccca aagagcgtaa gcgtcttccg atcgttccag
      541 ggtaaaagaa cggatcttct gctgatgggc ggtgtccaga gcgtcagcaa cccttaaaat
      601 ggaggccagt ttcgagacga tggcccggtc tgatttcgcg agcgacatat agggaacatg
      661 cgtctcgcgc ggcggcacgc gacggtgata tcggacgaca ttggcgacaa tattcttgtc
      721 agtccttcgc agtccaaaaa tatcagcggc gtctactaaa taaaaagaat gcttgtggtg
      781 tcctgacgga gaaacataaa taccgatgtc atggagtatc cccgaaacct cgagcaagag
      841 ccgctcgcgg gaacccatgc cgtgatcctt tgccaggaga tcataaagtt tcaaggccag
      901 cgaggccacg caaaccgcgt gagccttgtc atacatgtat ttcgcgccca gatgatgggt
      961 ggaattcagg acctgcctcg cgacatcggt ccttcggtaa cctgaaacca gttgcgctat
     1021 ttcgagcaac agaccgtccc ggatacttaa tttggggata atgatctcct cggccttagt
     1081 ttcattcagg aaatttaaat aaaagagaag tgcgggataa agggattccg cgtgaccgta
     1141 atcaagggag tactgcgtcg cgatctcctc cggggtcatt ttgcctatcc ttgacacaaa
     1201 tccgacaaac tccttttttt caagaaccgt aaaccggtcc acggtatccg gaagtaactg
     1261 ctgagataca aacctcacat cgcctccaag ggcgataaat gtatcgattc gctccataga
     1321 gacttcacgc gccacgttct ttgtgatatc ccgtatgctt ttttttagga cacgctggat
     1381 gaaatcctca tctcccttgg cggccaaacc ctgttccggc aacctcacgg atccgatcgt
     1441 cagcgttcgc gtcacgctaa cctgcccttg ctccataagg atcatttcgg tactgccgga
     1501 acccacctca acgatcaggc aattcttttt ttcaagattt attttttcgt ccagcgcgct
     1561 ttcgacggcc acgagctcaa gacgattctc ctccgcccct tccagaattt cgacctcaat
     1621 gccggtacgg acataaatct gatcgataaa attatcccga ttctgggcct cccggaccgc
     1681 gctcgtggcg atcgcgtgga ctcgcttgat cccatagccg tcaatcacgg atttaaaatc
     1741 ccggagaatg ttgacgccct gacgcatcgc cgcgttgctc aggcgtcccg ttttgaaaac
     1801 gtccttgccg aaacgaaccg gcttttggag ttgctccaga taacgaatct cgttcttgtt
     1861 cccaacctcg gcgatcacca tacggatcgc gatggtcccg atatcaatca cggcagcaat
     1921 atgtttagtg gaaatttcct gatcgggcat agcgcgcctt attttttggt cgttttcaaa
     1981 acttcacagg ccattttgga ggccccaaaa atgaccgcgt tatccttgag cttggaggga
     2041 acaactttaa gaaccttcac cagcggcttg agggcgtaag cctctatccc ggaacggacc
     2101 tcgggaagaa tgatctggcc catcgcctcc atcatgcctc cgccaagaag cacaagttcc
     2161 ggatttaaaa cgttcaccac gttcgcgagc gcaactccga gccaccggga acggcttcgg
     2221 atcagcgctt ccagggcggt atctcccgct ttaatggcac gggataaagc gccgctacga
     2281 atctttttga cgttatagcc gatctcatca agcaaatgcg ggcatttctg ccgtagcaag
     2341 agaaaccccg cttccgaggc gatcgcgttt cgaccgacca gggcgccgac tttctggcgg
     2401 gatatttcag agccggaaca agaaggca
//
LOCUS       DAOSIO010000215         2396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_38263_length_2396_cov_3.819308, whole genome shotgun sequence.
ACCESSION   DAOSIO010000215 DAOSIO010000000
VERSION     DAOSIO010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38263_length_2396_cov_3.819308"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="PK590_07255"
     CDS             complement(<1..1082)
                     /locus_tag="PK590_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOG24431.1"
                     /translation="MNRIRKVLMIFSKEMAGYFNSSIAYIFLIIFTLLNGGLFMTQFF
                     VIGRADMRSFFYGLPMLLCIFLPAVSMRLWAEEKRGNTQELLLTFPMAPHELVLGKFL
                     ASFCFYLLALACTAPVPLMLMVLGQPDLGPILGGYIGAAALGSFFLAIGILVSGFCRD
                     QIVAFILSMMICFGFYLIGTDFVAVSIDGWVPGLGSFLRQMLGAADHFAAFSKGVVDN
                     RDLLYFALGTVVCLVLNGFWIEGRMRPRAGSIFATAAVICTAIFLVSNWLVLGIPLGR
                     FDLTEGKVYTISEPTRKILRELKSPVMAKFYVSSSEKMPTALKTLEQDVRDKLDEIRI
                     ASEGRFQYKVYPMDVSDIVAKTAREE"
     gene            complement(1079..1813)
                     /locus_tag="PK590_07260"
     CDS             complement(1079..1813)
                     /locus_tag="PK590_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG24432.1"
                     /translation="MIDVDQLTMRYGHVLALDRVSFQAKPGEILGLLGPNGAGKSTAM
                     RILTTYLYPSAGTATVHGADIIKDSVKVRQLIGYLPESVPLYGYMNVEEYLRFVGAAR
                     GLSGARLKERLKWVMESCQLRKVWRQPASELSKGYGQRVGLAQALIHDPKVLILDEPT
                     SGLDPIQILSIRSLIKSLVQDKTIVFSTHILQEVEALADRIVIINEGKLVASGTREEL
                     SRKVGKPGASLEDVFITLLAPQGVVE"
     gene            complement(1975..>2396)
                     /locus_tag="PK590_07265"
     CDS             complement(1975..>2396)
                     /locus_tag="PK590_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HOG24433.1"
                     /translation="SALNGVSLSGIFLAYTASSIAMTFFIAAGTFAGVSIYGWVTKQD
                     LTSVGSFCGMALWGLIIASIVNIFFQTPTFYWILSYAGVAVFIGLTAYDTQKLKMIHQ
                     NARAVPGQIAIFGALTLYLDFINMFLYLLRIFGRRNN"
BASE COUNT          680 a          669 c          566 g          481 t
ORIGIN      
        1 ttttcttcac gggccgtttt ggcgacaata tctgaaacat ccatcggata gaccttgtac
       61 tggaaacgac cctcggaggc aatcctgatt tcatccagtt tgtcacgaac atcctgttcg
      121 agagtcttca gcgctgtcgg cattttttcg gaagacgaca cataaaactt ggccatcacg
      181 ggagatttaa gctctcgcaa aatttttcga gtgggctcgg aaatcgtgta aaccttccct
      241 tcagtcaggt caaatcggcc aagaggtatc cccaggacaa gccagttgga aacgagaaaa
      301 atagccgtgc atatcacggc ggccgtcgcg aaaatcgaac ccgctctggg ccgcatcctt
      361 ccttcgatcc agaatccgtt gagcaccaga caaaccaccg tacccagcgc aaaataaagc
      421 aggtcccggt tatccacgac ccccttggaa aacgcggcaa aatggtcggc ggcacccagc
      481 atctgtctca ggaaactccc gaggcccggc acccatccgt caatcgaaac cgccacaaaa
      541 tccgtcccga tgagataaaa cccgaaacag atcatcatac tgagaataaa agcgacgatc
      601 tggtcccggc aaaatccgga aacaagaatt ccgatcgcca aaaagaaaga tcccaacgcc
      661 gccgccccaa tatatcctcc caaaatcggc cccagatccg gctgtcccaa caccattaac
      721 atcagcggca ccggcgccgt acaggcaagg gccagcaggt aaaaacaaaa actggcgaga
      781 aactttccaa ggaccagttc atgaggggcc atcggaaacg tcaggagaag ctcctgggta
      841 ttgccccgtt tttcctccgc ccaaagcctc atggaaacag cgggaagaaa aatacacagt
      901 agcatcggca gtccgtagaa aaacgaccgc atgtccgcgc gaccgatcac gaaaaactgg
      961 gtcatgaaaa gcccgccgtt gaggagcgta aaaataatca gaaaaatata ggcgatggac
     1021 gagttaaagt aacccgccat ttccttacta aaaatcataa ggactttccg aatccggttc
     1081 attccacaac cccctggggc gcgagcagag tgataaaaac atcttccagg gacgcccccg
     1141 gttttccaac tttccgggac aattcttccc ttgtccccga ggccaccagc ttcccttcgt
     1201 taataatcac aatccggtcg gccagcgcct caacttcctg gagaatatgg gttgaaaaaa
     1261 caatcgtctt gtcttggaca aggcttttga tcagggaacg aatactcaga atctgtatcg
     1321 ggtcaagccc cgaagtgggc tcatcaagaa tcaacacttt cggatcgtga ataagcgcct
     1381 gggcaagccc aacccgttgg ccgtatcctt ttgaaagttc cgaggccggt tgcctccaga
     1441 cttttcgaag ctggcaggat tccatcaccc attttaaccg ttccttgagc cgggcgcctg
     1501 aaagtccccg ggccgctccc acaaaacgaa ggtactcctc aacgttcatg taaccataaa
     1561 gaggaacgct ttcgggaaga taacctatga gctggcgcac cttgacagag tctttgataa
     1621 tatccgcgcc atgaactgtg gccgttccgg ccgatgggta gagataggtg gtaaggatac
     1681 gcatggcggt agatttcccc gcgccgttag gcccaagcaa tccgagaatt tcaccgggct
     1741 tcgcctgaaa ggaaacacga tccagcgcca ggacatgtcc gtaacgcatg gtaagttgat
     1801 cgacgtcaat cattgatttc aaatcccagt ctggtttttc cgaaaactca actgcctggc
     1861 ctttcgggga gtttttcccg aggcgcccac ccggaggtat cccgcctcag acgggatacc
     1921 atagggttgg cttaaaaaac aagcgagatg gggggagtaa aacttcccga cctctcaatt
     1981 attcctacga ccaaaaatac gcaggagata gagaaacata ttgataaaat ccagataaag
     2041 cgtcagggcg ccaaaaatgg cgatctgccc cggaacagcc cgggcgtttt ggtgaatcat
     2101 cttgagcttc tgcgtgtcat aagccgtgag tccaatgaag accgccactc ccgcgtaaga
     2161 gagtatccag taaaaggtgg gggtttgaaa aaatatattt acgatgctcg cgataatcag
     2221 cccccaaagc gccatcccgc agaaacttcc aacggaggtc aggtcctgtt ttgtaaccca
     2281 gccatagata ctgactcccg caaaggttcc cgccgcgata aaaaacgtca tcgcgataga
     2341 cgacgcggta taagccagaa aaatccccga gagactgacc ccgtttaagg cagaaa
//
LOCUS       DAOSIO010000216         2377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_38606_length_2377_cov_3.928941, whole genome shotgun sequence.
ACCESSION   DAOSIO010000216 DAOSIO010000000
VERSION     DAOSIO010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38606_length_2377_cov_3.928941"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /gene="argC"
                     /locus_tag="PK590_07270"
     CDS             complement(<1..828)
                     /gene="argC"
                     /locus_tag="PK590_07270"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOG24434.1"
                     /translation="MLNCAVIGATGYTGSELTRLLLAHRSVRITHLTTRQEKHIPLAE
                     VLPSIPKDVDLRITPYDFEDVKKKADLVFLCLPHTEAMVTGDRFLQAGKIVIDLSADF
                     RLKDPALYPKWYGVEHAFPARLRQAVYGLCEVNREKIKKADLIANPGCYPTGPSLALW
                     PLFKKKMIRTDFVIINAASGVTGAGKKLVAATQYCEANENYNAYKVNRHQHIPEIEQN
                     CSEAAGKEVLITFIPHLLPLSRGILSTIYVEPSKKTLTEEAIRKVLEAVYSQDPFVRI
                     "
     gene            1058..1759
                     /gene="rnc"
                     /locus_tag="PK590_07275"
     CDS             1058..1759
                     /gene="rnc"
                     /locus_tag="PK590_07275"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOG24435.1"
                     /translation="MTKTPKLTKTRLRKGLGLSVKNLSLFDAALTHPSFRNENQFAEK
                     LADFDRLEFLGDSILNEVICQRLYAGFPQADEGMLSKLRSTLVSQRVLGRIAAKLRLH
                     TFMRFGKSLRQAMKTPYKHKLMTDALEAVFAAIFFDLGRKKTEAFILKHYAEYFDSKK
                     LFRLDPNPKSTLQELTLKRWKKIPVYVHKSSSKGITTVVSVDRKRKASGFHKIKKESE
                     LKAARALLSLLRREK"
     gene            complement(1781..>2377)
                     /locus_tag="PK590_07280"
     CDS             complement(1781..>2377)
                     /locus_tag="PK590_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOG24436.1"
                     /translation="NLDLFLNRAGLEKQWFGGSTILKFSVDGSGWGRDAWTSSLKGRG
                     QLNLTEGRFGFMDPVGALSSIEPLSSLASRMKSPNSFNELDFNWVLSGGKVETEDFLV
                     KSSDYVVDGEGTLGFDGLLNLRWDMYLPTSLAAEIFPGMAETFHRETRAHLGPITLLI
                     SGLADSPHIKPDPARAEELMQMIREEKARSLLRELVLD"
BASE COUNT          632 a          633 c          536 g          576 t
ORIGIN      
        1 aatcctgaca aaagggtcct gagagtaaac cgcctccagc accttccgga tcgcctcctc
       61 ggtcaatgtt tttttggaag gttcgacgta gatcgtcgaa agaatcccgc gagaaagcgg
      121 cagaagatgc ggaataaaag taatgagcac ttccttgccc gcggcttcgg aacaattctg
      181 ttcaatctcg ggaatatgct ggtgccggtt cactttgtag gcattgtaat tttcattcgc
      241 ctcacaatat tgggtcgccg ccaccagttt tttcccggcg ccagtgaccc ccgacgcggc
      301 attaataatg acgaaatccg ttcggatcat ttttttctta aaaagcggcc aaagggcaag
      361 agacggcccc gtcggataac acccgggatt agcgatcaaa tccgcttttt tgattttttc
      421 ccggttcact tcacacaaac cgtaaacggc ctgtcgaagc ctcgcgggaa aggcgtgctc
      481 aaccccgtac cacttggggt agagggccgg atctttcagc cggaaatccg cggaaagatc
      541 gatcacgatt ttaccagcct gaaggaaacg atcacccgtc accatggctt ctgtgtgagg
      601 gagacaaaga aacacaagat cggccttctt cttgacgtct tcaaaatcat acggagtaat
      661 acggaggtcc acgtccttgg ggatcgatgg caaaacctca gctagcggaa tatgtttttc
      721 ctgccgcgtt gtcagatggg tgattcggac acttcggtgg gcaagcaaca atctggtaag
      781 ttcagatcct gtatatcccg tcgcgcctat tacagcacag ttcaacattt gacttaatgc
      841 ttctcctttt cgggaaaaaa agacactccc ctagttgacg ttccctccac gtgagcgggt
      901 attatagaaa tagctctatc ctttgtcaat ttaagaagtt acgacaactc ttagaccagc
      961 aaaagggggc ttcattattc ataactctat actctatagt tacttacgct attttataat
     1021 tgacagccct gcgtgaaaac attatgttaa atctcccatg accaagacgc caaaactcac
     1081 caaaaccaga cttcgtaaag gactcggcct ttccgtgaaa aatttgtccc tctttgacgc
     1141 ggccctgacg catccttcgt tcagaaacga aaatcaattc gcagaaaaac tggccgattt
     1201 tgaccgtctt gagtttcttg gggattccat cctgaacgaa gtcatctgtc agcggcttta
     1261 cgccggcttc ccgcaagccg atgaaggtat gctctccaaa ctccgctcca ctcttgtttc
     1321 acagagagtc cttggaagaa tcgccgcgaa actgcggctc cacacattca tgcgctttgg
     1381 gaaaagtctg cgccaggcta tgaaaacccc ctacaagcac aagcttatga ctgacgctct
     1441 tgaggcggtt tttgcggcaa ttttctttga tctcggaaga aaaaaaacgg aggcttttat
     1501 cctcaagcac tacgcggagt attttgactc caaaaaactt ttccgcctgg accccaatcc
     1561 caaaagtacg ttgcaggagc tcacgctgaa aagatggaaa aaaatccccg tttacgttca
     1621 taaatccagt tcgaagggaa tcaccacggt tgtttccgtc gaccgaaagc ggaaagcctc
     1681 cggtttccac aagatcaaaa aggaatcgga actcaaagcc gcccgggcat tactgagtct
     1741 acttcggcga gaaaaataag agagctgcct tttttttgtt ttaatccagc acaagttcgc
     1801 gaagaagcga tcgagctttt tcctcgcgga tcatttgcat caattcttct gcccgggccg
     1861 gatcaggctt aatgtggggg gaatcggcca atcccgagat gagcaatgtg atcggcccca
     1921 gatgggctct ggtttccctg tgaaaagtct ccgccatccc agggaaaatt tccgccgcca
     1981 gtgaagtcgg aaggtacatg tcccatcgca ggttcaacaa accgtcaaaa ccgagcgttc
     2041 cttcaccatc caccacgtaa tcggaacttt tgacgaggaa atcctccgtc tccactttcc
     2101 cgccggaaag gacccaatta aaatcaagct cgttaaagga gttgggtgat ttcattctgg
     2161 acgcgaggga gctcaagggc tcgatggacg acaaagcgcc aacagggtcc ataaaaccga
     2221 accgcccttc agtaagattt aattgtcccc gccctttgag cgaagaggtc cacgcgtctc
     2281 gcccccaacc gcttccatcg acggaaaact tcaggatcgt tgaacctcca aaccattgtt
     2341 tttccaaacc cgcccgattc aaaaagagat caagatt
//
LOCUS       DAOSIO010000217         2318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_39964_length_2318_cov_2.567388, whole genome shotgun sequence.
ACCESSION   DAOSIO010000217 DAOSIO010000000
VERSION     DAOSIO010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39964_length_2318_cov_2.567388"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="PK590_07285"
     CDS             complement(<1..1132)
                     /locus_tag="PK590_07285"
                     /inference="COORDINATES: protein motif:HMM:NF036484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HOG24437.1"
                     /translation="MGAIELRGVKTHNLKNLQLKLPRRKLYVVTGVSGSGKSSLAFDT
                     LYAEGQRRYLESVSAYARQFLERMPKPDVEFSSGIPPALAIQTKNVVSNARSTVGTQT
                     EINDYLRVAFARAGRLICPECEIEVEKYDPVSIARRLLQDFLEKTVRIVALVPLGKAG
                     MKYLSENVRELERQGFSDFFCDGEWKNGDSLLSAKQKFSELRVSIDVLPVNEKNRERL
                     TDSLELGFRYGKGNVQVWCQGRELKFSEILQCVSCGKKFPEPVANLFSFNSPLGACPA
                     CQGFGRVITIDRDLVVPDASKSIREGAIEPWTKASTRWERGQLLDFCARKRIPVQRPW
                     ATLKKEHQKWIFEGFPGDDYFSLRDFFDYLNKKIYKMHVRIFL"
     gene            complement(1151..1540)
                     /locus_tag="PK590_07290"
     CDS             complement(1151..1540)
                     /locus_tag="PK590_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24438.1"
                     /translation="MRTIGMILTGALFCFYFSGCATVEKMLPFHDEVLVYELPLDLTY
                     LRTTEAMETLPGWVIESTDKEKGVIAARNVEYSRLDDSDTRTVTVLVKRVDRTHTSVT
                     LAPESQRSLSGGDMLKAIDTMLSAELK"
     gene            complement(1563..>2318)
                     /locus_tag="PK590_07295"
     CDS             complement(1563..>2318)
                     /locus_tag="PK590_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24439.1"
                     /translation="YADGKKIRARTYAELRMNGGYPQGNQLVLRNSLQYQINRDLAFL
                     TKFDYGATYLFDLVDNNKQMNLGNFADFSVAFAYRPVGLDHLNFLLKYSYLRDIGNDM
                     QFMNGYYNDHMFHEIAHIVALDAAYDFNRYFGFVQKLAWKRSTLITQNRDVWSLARNT
                     LDETDVNNFLTVSRINFHVTRKWDVAAEYRLLWQSSAMDTMRHGMLFEVDREIYDYVR
                     LGVGYNFTDFSDDLRHSNDYQNSGPFVRMTGKF"
BASE COUNT          637 a          626 c          478 g          577 t
ORIGIN      
        1 ttaaaaaaat cctgacgtgc attttgtaga tttttttatt gagataatca aaaaaatcac
       61 gaagactaaa ataatcgtcc cccgggaatc cctcgaagat ccatttttga tgttcctttt
      121 taagtgttgc ccagggcctt tggaccggaa ttcgtttacg cgcgcaaaaa tccagcaatt
      181 gcccccgctc ccaccgcgta ctggccttgg tccacggttc aatcgcgcct tcgcgtatcg
      241 attttgaggc gtccgggacc acaagatccc tgtcgatcgt aatcacccgt ccaaacccct
      301 ggcaagcggg acacgcgccg agaggactat taaatgaaaa aagattagcg actggttcgg
      361 gaaacttttt cccgcaagac acgcattgaa gaatttccga aaatttcagt tctcggccct
      421 ggcaccaaac ctggacatta cctttcccat atcgaaatcc aagctccagc gaatccgtca
      481 gacgttccct gtttttttcg ttgaccggta acacgtctat actcacgcgc agttcagaaa
      541 atttctgctt cgctgacagc agggaatccc cgttcttcca ttccccgtca cagaaaaaat
      601 cactaaaccc ttgtctttca agttccctga cattttccga aagatatttc atccccgctt
      661 ttccgagcgg gacaagggcc acgattcgta ccgttttctc taaaaaatcc tgaagaagtc
      721 ggcgggcgat actgacagga tcatattttt caacttcaat ttcacattca ggacagatta
      781 atcttcccgc gcgcgcaaaa gcgacccgga gataatcatt gatttcagtt tgtgtcccga
      841 cggtagaacg cgcgttactg acaacgtttt tcgtctgaat cgcaagcgcg gggggaatcc
      901 cggaagaaaa ctccacgtcg ggtttaggca ttcgctcaag aaactggcgg gcataggcgg
      961 aaaccgactc cagatagcgg cgctgacctt ccgcgtagag ggtatcaaaa gccagcgagg
     1021 acttccctga accggagaca cccgtcacga cataaagttt tcgtcgaggg agttttagtt
     1081 gaagattttt gaggttgtgg gttttaacgc cacgaagttc tatcgcgccc aagacaatgg
     1141 ccttgggacg ctatttgagc tcggcgctca acatcgtgtc gatagctttc agcatatccc
     1201 cgccggacaa actccgctga gattcaggag ctaaagtaac cgacgtatgg gttcggtcaa
     1261 cccgtttcac aagcaccgtc accgtccgcg tatccgagtc atccaaccgg ctgtattcaa
     1321 cgttgcgggc ggcgatcaca cccttctcct tgtcagttga ttcaataacc catcccggca
     1381 aggtttccat ggcctccgtc gttctaagat acgtcagatc caggggcaac tcataaacaa
     1441 gaacttcatc atgaaaagga agcatttttt caaccgtcgc acaacctgag aaataaaaac
     1501 aaaacaaagc acccgtcaag atcataccaa tagttcgcat cgttaaatcc ccatttccat
     1561 cgttaaaatt ttcccgtcat ccggacaaac ggcccactgt tctgataatc attagaatgg
     1621 cgaagatcat cgctaaaatc cgtaaaatta tacccaacgc ccagtctcac ataatcgtaa
     1681 atctcacgat ccacttcaaa caacatcccg tgccgcatcg tgtccatagc ggacgattgc
     1741 cataaaagcc ggtattcggc ggccacgtcc catttccggg tcacatgaaa attgatccgg
     1801 ctcactgtca ggaaattatt tacgtcggtt tcgtcaaggg tatttcgagc caaagaccaa
     1861 acatcccgat tctgggtaat aagcgttgag cgtttccacg ccagcttctg cacaaagcca
     1921 aaataacggt taaagtcata cgccgcatcc agagcgacaa tatgcgcgat ttcatggaac
     1981 atatgatcat tgtaataacc gttcatgaat tgcatatcat tgccaatatc cctgagataa
     2041 ctgtacttta aaagaaaatt cagatgatcc agtcccacgg gacgatacgc gaacgcaaca
     2101 ctaaaatccg caaaattccc aaggttcatt tgtttattat tatcgaccag atcaaaaagg
     2161 tacgtcgcgc catagtcaaa ttttgtcaga aaagccaggt ctctattaat ctgatattgc
     2221 aaggaatttc taagcaccag ctgattcccc tggggatacc ccccgttcat ccgcaattcc
     2281 gcgtatgtcc gcgcccgaat ttttttccca tccgcgta
//
LOCUS       DAOSIO010000218         2287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_40655_length_2287_cov_4.393369, whole genome shotgun sequence.
ACCESSION   DAOSIO010000218 DAOSIO010000000
VERSION     DAOSIO010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40655_length_2287_cov_4.393369"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(391..>2287)
                     /locus_tag="PK590_07300"
     CDS             complement(391..>2287)
                     /locus_tag="PK590_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24440.1"
                     /translation="EKVREKWRIPSHEISCAREEKSGYIPYTWLGGIKVGQETELRIV
                     RDSQWVYSIQQKRRGKWQYIPGAEFSLKGNDAAEQVVMVGRSRREHIPSVRMVNPLGN
                     DLTVSRAHVLIQARVGANGDALFQFQRFSERGSVFASMFDSEIERYNRWVLLEEWKKF
                     SGRHGFFSGARTIACLMDRLGIIRGNAWVVDTVIPKDWETLVFRPGWKVVYFKFQNLK
                     VGRWRLPVFQWGTQKFFEPLRVLGTNQGQEEFPDPRGWAVLETSVAKINETLSRSESR
                     VQDILSESGIPNLPGLSSVKETPQMPATGSDPVSETTLSIHEPETQPRFLEAGRVNPQ
                     SAFNTVSLPPEIDGQLKKSGSLLPLIKVASVAGGTVNPTYYSGRKICEFLLGLIAPKT
                     VSLERTLAPEKIQKLQSRNRDAFSAAKNPGEPVASGGTIFMDETGLLDFASYDPQGFF
                     EFLNVLSASMTEGDPGKPLVTGGDQKLFKDLEMILKNKNAGLTVLQQGEVRGLMARVL
                     EVVPAGDDPGEVIRQRANRTGWVALLSNENLLAGLPEGANFAFKNGLDKENARLIMRS
                     LLSRMRVLASLLHKAPPESRAALLEQFEQRQLGGTRKSAGGYVFDDILALAESLMTSV
                     QAVARSA"
BASE COUNT          576 a          663 c          484 g          564 t
ORIGIN      
        1 tacgattgtt acggtacaaa cgacccgcct tacccacttg ttatccatca ttctttccat
       61 cgaaccccca cgagacgcct gacgcgtcta tttcaagcta tcagaactcc actccccgga
      121 cacactacca atcaagcgac ttaaatcccg ttccggacta aataacccac taaacagtca
      181 ttgatgataa tcttgttaca tactttgtta tcttctatct aaaaagtagc tgatgaatgc
      241 aaggatgtca ataactaata aaatttaata ataaagtatt tattgggctt tattttaact
      301 cttatttttc ataaaccgtt ttaaacaaat gatttagaaa aatagaggta tcagaggaga
      361 gaaatgaaca gactttagat actggttttt ttacgcggaa cgagcgaccg cctgcacgct
      421 tgtcatcaaa ctttcggcaa gcgcgagaat atcgtcaaaa acataccctc ccgccgattt
      481 tcgggtccca cccagctgac gctgttcaaa ctgctcaagc aaagccgccc gggattccgg
      541 aggcgccttg tgcaataatg acgcgaggac tcgcatccgg ctcaagagac tacgcatgat
      601 caatcgggcg ttttccttat ccagaccatt tttaaacgcg aagttcgcgc cttccggcaa
      661 tccggcaagc aggttttcat tcgacaaaag ggcgacccat cccgtccggt ttgccctctg
      721 gcgtatcacc tctcccgggt catcccccgc cggaaccact tccaacaccc gcgccatcag
      781 gccccgaact tctccttgct ggagtaccgt taaaccggcg tttttatttt tcaaaatcat
      841 ttcaagatcc ttgaataatt tttggtctcc cccggtcaca agcggcttgc caggatcccc
      901 ttccgtcatc gaggccgaaa gaacgttcag aaattcaaaa aagccctggg gatcgtagct
      961 cgcaaaatcg agtaaccccg tttcgtccat aaaaatcgtc ccccctgacg cgacaggctc
     1021 gccgggattt ttcgccgctg aaaacgcatc tcgattccgc gattggagtt tttggatttt
     1081 ttccggcgcc aatgtcctct caagcgaaac tgttttaggc gctatcaatc cgagcaaaaa
     1141 ttcgcagatc ttacggccag agtaataggt cggattcacc gtccccccgg ctacggaagc
     1201 aactttaatg agtggcaaaa gagaacccga ttttttaagt tgcccgtcaa tctctggcgg
     1261 caaggacacg gtattgaagg cgctttgcgg atttaccctg ccagcttcaa ggaaacgcgg
     1321 ttgcgtctcg ggctcatgaa tggacaaagt cgtttcactg acaggatcag aacctgtcgc
     1381 gggcatctgc ggcgtctcct tgaccgatga caaccccgga aggtttggaa tgccgctctc
     1441 cgaaagaatg tcctgaaccc ggctttccga gcgactcagg gtctcgttga ttttcgccac
     1501 ggaagtttca agtactgccc aaccgcgagg atctggaaat tcctcctggc cttgattcgt
     1561 cccaagcacc cgcaatggtt caaagaactt ttgagttccc cattgaaata ccgggagcct
     1621 ccaccggccg actttgagat tttgaaactt gaagtacaca accttccatc ccggacgaaa
     1681 aaccagagtt tcccagtcct tcggaataac cgtgtcaacc acccaggcat ttccccgaat
     1741 aatacccaat cggtccataa ggcaggcaat ggtccgggcg cctgaaaaaa aaccgtgacg
     1801 gccgctaaac ttcttccatt cttccaaaag aacccatcgg ttataccttt cgatttccga
     1861 gtcaaacatc gaggcgaaaa cactccccct ttctgaaaat cgctgaaact ggaacagggc
     1921 atccccgtta gctcccacgc gggcctgaat caggacgtgc gcgcgggaca ccgtcaggtc
     1981 atttcccagg ggattaacca tccggacact gggaatgtgc tcccgacggc tacggcccac
     2041 catcacgacc tgttccgcgg catcatttcc ttttaaagaa aactccgccc cgggaatata
     2101 ctgccatttg cccctccgct tctgctgaat gctgtaaacc cattggctgt ctcgcacaat
     2161 tctcaactct gtctcctggc caactttaat ccctccaagc caggtgtagg gaatatatcc
     2221 gcttttttct tcccgggcgc atgaaatctc atggctgggg attcgccact tctcccgaac
     2281 tttttcg
//
LOCUS       DAOSIO010000219         2277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_40886_length_2277_cov_6.550405, whole genome shotgun sequence.
ACCESSION   DAOSIO010000219 DAOSIO010000000
VERSION     DAOSIO010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40886_length_2277_cov_6.550405"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PK590_07305"
     CDS             <1..423
                     /locus_tag="PK590_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24441.1"
                     /translation="DGVIGVLLVVLMAGCATKGASGSKIVVLETSDVTRAHDVVGSVS
                     VSEQVAESAADTLKGLAGYATRDERVSSQIPSDTKAALNAKAVQYKEMIFEKLGTKAQ
                     SAGADAVIGARYHYVPAYVNLSQKATVSAEGTMIKYKK"
     gene            complement(611..1252)
                     /locus_tag="PK590_07310"
     CDS             complement(611..1252)
                     /locus_tag="PK590_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG24442.1"
                     /translation="MARTPKKRLAASAKTRKRNRKKSSQRILQAALKTFSKVGYDAAT
                     TKMISRQAGLNESLIHRYFTSKSNLLVEVTYSCIDALSKEKPYPPADTPQEEIYRFLV
                     NRFENYNKNIRFFRVILSRILLDSRTGSELQKRKPSDLFFAERLTLFQKKGLIRSDLN
                     INELVSLIAHQASAMTIAKLILSHKSPEYCKKQLLFFSKNLTRGIVAPPRKHP"
     gene            complement(1428..1844)
                     /locus_tag="PK590_07315"
     CDS             complement(1428..1844)
                     /locus_tag="PK590_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOG24443.1"
                     /translation="MEIIAKDDHAAIEEAVRNYWPEALSRDCIFHLLQNLAKKPKGFW
                     QKYVILRDASWLFQAQNAGEFQKWAALFRKKYARIINLPALKYFVSKWHGATRFYALE
                     PRFWPAAKTTNRLERLFGELRRRIRAFRRFPNTLSC"
BASE COUNT          530 a          468 c          649 g          630 t
ORIGIN      
        1 gacggtgtga ttggagttct gcttgtggtt ttgatggcgg gttgcgcgac gaaaggcgct
       61 tcgggctcga agattgttgt cctggagact tcggatgtga cgagggccca tgacgtggtg
      121 ggctcagttt cggtgagtga acaggtcgcg gagagtgcgg ccgatacgct caagggactt
      181 gcgggttatg ccaccaggga tgagcgtgtg tcgagtcaga ttccgtcgga tacgaaagcg
      241 gctctgaatg ccaaggcggt tcaatacaag gaaatgattt ttgaaaagtt gggaacaaaa
      301 gcccaatcag ccggtgctga cgcggtcatt ggtgcccggt accattatgt tcccgcttac
      361 gtaaaccttt cgcagaaagc gacggtgtct gccgaaggga cgatgattaa atacaaaaag
      421 tgaattgcct cgcccctgat tcgggagtct gggaaaatat ccgaagggcg ggaggggttg
      481 gctggcctgt gaaaacttcc agaagtcagc gtgtctcagg attcagtgag tttaacggcg
      541 gagtggacgg ttttccgcgc gggagagttc ctgtttttaa acgggtgaga gtccccaggg
      601 cgcgaaacca tcagggatgt ttccggggag gcgccacaat tcctcgagta agattttttg
      661 aaaaaaacag aagctgtttt ttgcagtatt ccgggctttt gtgagacagt atgagcttgg
      721 caatcgtcat ggcagaagcc tgatgggcga ttagagagac aagttcgttg atgttcaggt
      781 cggatcgaat caaccctttt ttttgaaata atgtgagacg ttccgcgaaa aaaagatccg
      841 agggtttccg tttttgaagc tctgatcccg tccgggaatc gagcagtatc cttgagagga
      901 tgacgcgaaa aaaccggatg tttttattgt agttttcgaa tctgttgaca aggaaacgat
      961 aaatctcttc ctgcggagtg tcggcgggag gataaggctt ttccttgctg agagcgtcaa
     1021 tgcaggaata ggtaacttcg acgagcagat ttgatttgct tgtaaaatac cggtgaatca
     1081 gtgactcgtt aagcccggcc tgccttgaaa tcatcttcgt ggtggcggca tcatagccta
     1141 ctttcgagaa ggttttaagt gcggcttgaa ggattcgctg ggagcttttc tttcggtttc
     1201 gcttcctggt tttagcggag gctgcaagcc tcttctttgg ggttcttgcc atagtatttt
     1261 tattttgcat tccctggatg cggatcatcc acccacatta tttaaagaga aaaggttgaa
     1321 agtcaattga tgtaaggacg ctgtcagata aatcgccgac tttgggaagc cggtagtatc
     1381 ggatttttgg tttgattgag tttgagtaaa agagcgaaag tccatcgcta acagcttaag
     1441 gtgttgggga accgccgaaa ggccctgatc ctgcgtctca attcaccgaa taatctttcc
     1501 agtcgattag tggttttggc ggcgggccag aagcgtggtt ccagcgcgta gaatcgtgta
     1561 gcgccatgcc atttgctcac gaagtatttc agggccggga gatttatgat gcgagcgtat
     1621 tttttccgga acagggctgc ccatttctga aattccccgg cgttttgggc ctgaaaaagc
     1681 catgaggcgt ccctgagaat gacatatttt tgccagaatc ctttcggttt ttttgccaga
     1741 ttctggagca ggtgaaagat acagtcccgg gagagggcct cgggccagta gtttctgacc
     1801 gcttcctcaa ttgcggcatg atcgtccttg gcgattattt ccaccgcggg aagtcagcaa
     1861 aatacattgc ggcacgggtg taagtggttg cttgacaata gtcgccaaat atcttacatt
     1921 tggaaaaaat gtaagtggtt aattacatcc cccttcagag agagaaatca tgctaattca
     1981 aagcggtgct tcgcaaaccc ggttcttgtt gtccctgata acggtttttc catgtctgaa
     2041 aagattcttc cgtcgtttct ttcccgctcc gggagtttta ttttttgttt ttttgatttc
     2101 gttgatgggg ggatgtaaag ccaaagatac gtcccggacg atgattcccg aggtcgcggt
     2161 cgtgacttta caggctgaaa aagtgtccat caccgatgaa ttgccggggc gtaccagcgc
     2221 ttttttgata gctgaaatcc gtccccaggt gaacggtatt atccagaaga ggttgtt
//
LOCUS       DAOSIO010000220         2275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_40938_length_2275_cov_3.893243, whole genome shotgun sequence.
ACCESSION   DAOSIO010000220 DAOSIO010000000
VERSION     DAOSIO010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40938_length_2275_cov_3.893243"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PK590_07320"
     CDS             complement(<1..314)
                     /locus_tag="PK590_07320"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOG24444.1"
                     /translation="MYKIVLIRHGESTWNKENRFTGWTDVDLSEKGLKEATEAGQLLK
                     KEGYCFDIAFTSVLKRAIRTLWNVLDQMDLMWIPVVRDWRLNERHYGSLQGLNKSETA
                     AK"
     gene            complement(413..1663)
                     /locus_tag="PK590_07325"
     CDS             complement(413..1663)
                     /locus_tag="PK590_07325"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG24445.1"
                     /translation="MYIHPASEPPVSASFFGLGIAPQILDTLTRLGFKVPTPIQQKAI
                     PVAIGGKDIVGIAQTGTGKTMAFGIPMLQRLAQLKGRGLILVPTRELALQVHQSLHKI
                     SPQTKYAILIGGASMNNQIQELRKHPRIIIATPGRLLDHMEQRYVFLDKVGILVLDEA
                     DRMLDMGFAPQINQILRKLPRERQTMLFSATMPEQIMRIASQHMKLPVSVEIAKSGTV
                     AEKITQEVFIVKKEAKRQLLQKILAQYHGTVLVFSRTKIGAYKISRAIRDMGHSVAEI
                     HADRTLGQRRQALDGFKSGKYRILVATDIAARGIDVQGIELVLNYDLPDESENYVHRI
                     GRTGRIGHKGHAISFATPEQGKEVKNIEKLMRMAIPISEHPEMPIESFITSKPQVVAS
                     FSGRHFSRGRRSRGFISSAFRKSR"
     gene            1880..1955
                     /locus_tag="PK590_07330"
     tRNA            1880..1955
                     /locus_tag="PK590_07330"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1913..1915,aa:Asn,seq:gtt)
BASE COUNT          511 a          556 c          630 g          578 t
ORIGIN      
        1 tattttgccg ccgtctcgga tttgtttagt ccctggagcg agccgtaatg gcgttcattg
       61 agccgccagt cccgaacgac gggaatccac atcaggtcca tctggtcgag cacgttccag
      121 agagtgcgaa tggcccgctt gaggaccgac gtaaacgcga tatcaaaaca atagccctct
      181 ttcttgagaa gctggcccgc ttcggttgcc tctttcaaac ctttttccga caaatcaacg
      241 tcggtccagc cggtgaaccg gttttctttg ttccaggtac tttcaccatg gcggattaga
      301 acgatcttat acatggttat atcctccttg agtcttgagt caggaatgtg tccgaagggg
      361 gatagtgtac cttatcttga aggagttttg aaaaaaaata ccggaaaagg tcttagcggg
      421 atttgcggaa ggcggaagag ataaaaccgc gggaacgacg gccccggctg aaatgacggc
      481 ccgaaaaaga agcgaccacc tggggtttgg acgtgataaa gctttcaatc ggcatttccg
      541 gatgttccga gatcggaatg gccatgcgca tgagcttctc gatattttta acttcctttc
      601 cctgctccgg ggtcgcgaag gaaatggcat gtcccttgtg gccgatcctt cccgtgcggc
      661 cgatccggtg aacgtaattt tctgattcat ccggaagatc ataattcagc accagttcga
      721 ttccctgaac gtcaatgccg cgggcggcaa tatccgtcgc gacaaggatc cggtatttcc
      781 ccgatttaaa tccgtccagg gcctgccgcc gctggccgag cgtgcgatcg gcatgaattt
      841 cagccacgct atggcccatg tcccggatgg cccttgagat cttgtaggcg ccgatttttg
      901 tccttgaaaa aacaaggacg gtcccgtgat attgcgcgag aattttctgg agtaattgcc
      961 gtttcgcttc ttttttgacg atgaaaactt cctgtgtgat cttttccgcg accgtgccgg
     1021 acttcgcgat ctcgaccgag acggggagct tcatgtgctg ggacgcgatc cgcatgatct
     1081 gctccggcat ggtggccgaa aaaagcatcg tttgccgttc ccgcgggagt tttcggagga
     1141 tctggttgat ctggggagca aaacccatat caagcatccg gtcggcctca tcaaggacca
     1201 gaatgccaac tttatcgagg aatacgtatc gctgttccat gtggtcgaga aggcggcccg
     1261 gggtggcaat gatgatcctc gggtgcttgc ggagctcctg gatctggttg ttcatcgaag
     1321 cgccgccgat cagaatcgcg tattttgtct gcggagaaat tttgtgaagg ctctggtgga
     1381 cctggagcgc gagttcacgg gtcggaacaa gaatcaggcc gcggcccttg agctgggcca
     1441 gacgctggag catcggaatc ccgaaggcca tcgtttttcc ggttccggtt tgggcgatcc
     1501 cgacgatgtc ttttcccccg atggccacgg ggatggcctt ttgctgaatc ggggtcggaa
     1561 ccttgaagcc gagcctggtc agggtatcaa gaatttgcgg agcgatccca agcccgaaaa
     1621 aagacgccga aaccgggggt tcacttgccg gatgaatata catggggaga agtataggga
     1681 caaatcccgc aaatagcgag atttttaaat caaattttaa tccatttccc gcgcgtcggc
     1741 gcctctcttg aaaacactga ttgacccttt gacgctaatc cctataatta cagttcctgg
     1801 ttgataaatt gtagggtgcg ggtggcaagt cgggggatac aaacctgttt ctttgccgcc
     1861 tgataccgtt cttttacatt cccctgtagc tcagttggta gagcaagcgg ctgttaaccg
     1921 ctttgtccct ggttcgagcc caggcggggg agccattata agaaaaaaag cgtagggtct
     1981 ttagatcctg agcttttttt atatagacgg ttttccaccg ccagtcctga attccttaca
     2041 ggttctatag aatcagggca ggcgggggag ccattttgga cacttggacg aacctgtcga
     2101 taaaggtagg taagaatctg agtgtcaggc aaacaagagc ctcgcttccg tttggaggcg
     2161 aggctcattc gttatgaggg ttatttgagg gaaaaaaacg tttatatcat tgccggcccg
     2221 aatggttccg gcaaaacgac tttcgcaaag ttatttctgc ctgagtatgt caatt
//
LOCUS       DAOSIO010000221         2275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_40944_length_2275_cov_3.332883, whole genome shotgun sequence.
ACCESSION   DAOSIO010000221 DAOSIO010000000
VERSION     DAOSIO010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40944_length_2275_cov_3.332883"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="PK590_07335"
     CDS             <1..140
                     /locus_tag="PK590_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOG24446.1"
                     /translation="MRSARMGVFQITPVNSTSVSDWGENDTSSVEKKVTAVVNASFSI
                     E"
     gene            148..606
                     /locus_tag="PK590_07340"
     CDS             148..606
                     /locus_tag="PK590_07340"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG24447.1"
                     /translation="MDFDENDDPMDCLKPERLLEEIRGLEGEIRWKPDDSELYFQLGV
                     YSCCLKDNEKAAYSYLKALELDPEMHQAHYLLGEHYFSKKRYWDAAMEFGNYVSAEPD
                     DPDGTLYLKFGIACLRLGRTEDAKRHLEKARLDKDSGRAGRAAKLLSRIS"
     gene            1041..>2275
                     /locus_tag="PK590_07345"
     CDS             1041..>2275
                     /locus_tag="PK590_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOG24448.1"
                     /translation="MEIIKIETSKIRMAEYNPRVALKPGDSEFEKLDRSIKEFGFVEP
                     AVWNKRTGNLVGGHQRLAVLKAQGVTEVEVSVVDLPLEKEKALNIALNKIKGGWDEEK
                     LAGILQELSQVPDFDVSLTGFESVEISQLFDEYLTPDPEDDFDLKKEAAEGIITPITQ
                     KGDLIELGRHRLLCGDSSSPEDLKRLLGDQKVRLLHTDPPYNCRYDSTCRPGQDKSDS
                     KWKPIANDWAEQTDYEAWLAKFFETVKPHLAEGAPLYVWNGHRQFGPMHAILTNLGFH
                     VSNVITWVKPSASPGYADYKMQSEFCLYAWLEGNGSHHWFGGNSESNVWECPRDAAHT
                     LIHPTTKPVALAQRAIKNSSQRGDLILDCFAGSGSTIMAAQSMERVCCAMELEPAYCD
                     AIVRRFIKKYGHESISAEIR"
BASE COUNT          613 a          521 c          585 g          556 t
ORIGIN      
        1 tcatgcgttc cgcccggatg ggtgtgttcc agatcacgcc cgtgaactcc acttctgttt
       61 cggattgggg cgaaaatgat acaagttcgg tcgaaaagaa agtcaccgcc gttgtgaacg
      121 ccagcttttc gatagaatga gtgacagatg gattttgacg agaacgacga tcctatggat
      181 tgcttaaagc ccgaacgcct tcttgaggaa atccgagggc tggaaggaga gatccggtgg
      241 aagcctgacg attcggagct ttactttcaa ctcggcgtct attcctgctg cctcaaggac
      301 aacgagaaag cggcttattc ctacctcaaa gcgcttgaac ttgatccaga aatgcaccag
      361 gctcattatc tattgggtga gcattatttt tcgaagaagc ggtactggga cgcggccatg
      421 gaattcggga attatgtttc agcggaaccg gacgaccccg atgggacgct ttacctgaag
      481 ttcgggatcg cgtgtctgcg tttggggcgg accgaagacg ccaagcgcca tctcgaaaaa
      541 gcacgactcg acaaagactc cggccgcgcc gggcgtgcag ctaaactcct gtcccggatt
      601 tcttaaaagg caaggttttg tcgtcttttg tttaaaagat gtcagagtgg ttaatattat
      661 tttctttaaa atagtaaaat tgtttattcc tcccgtagcc ccacgtctct tttaagctcg
      721 tcataactat cttgctcttc tcccgtcaag ctctctcaag ggcgcacgct ctcggagcgc
      781 attccacagg gtgataatat tttctcgccc cgattacata tagggtaaac aaatcaatcc
      841 aaaaaaacca ggggtgtgtc tttttgacgc ggaaatgaaa taataaaaaa attataaata
      901 tattcatttt caaaaattta tatatcggga ggtatataaa tatacagggg aagaaagtta
      961 agggttctct ttcttcctct gtgttttgac gccaaaagag cccaaaacaa aattaataaa
     1021 tcaataaaaa ggagataaca atggaaatta ttaaaattga aacatcaaag atccgtatgg
     1081 ccgaatacaa tccgcgcgtt gctctcaaac caggggactc cgagttcgag aagttggacc
     1141 gtagcatcaa agagttcgga ttcgtcgagc ctgcggtttg gaacaaacgg accggcaacc
     1201 ttgtgggcgg tcatcagcgt ctagcagttc tgaaagctca gggtgttaca gaggtcgaag
     1261 tgagcgtggt cgatctccct ctggagaagg agaaggctct gaatatcgcc ctcaacaaaa
     1321 tcaaaggagg ctgggatgag gaaaagttgg cagggattct tcaggagcta agccaggtgc
     1381 cagacttcga tgtctcactt accggatttg aaagtgtcga gataagccag cttttcgacg
     1441 aatatctgac ccccgatccg gaggatgatt ttgatcttaa aaaggaggcg gcggagggga
     1501 tcattacacc tattactcaa aaaggtgatc tgattgagct tggccggcac cggcttttgt
     1561 gcggggattc aagctcgcct gaggatctca agcgcttatt gggggaccaa aaagtccgtc
     1621 tgctgcatac cgatccacct tataactgcc gctacgattc cacatgtcgt cccggacagg
     1681 ataaaagtga ttccaagtgg aaacctatcg caaacgattg ggccgaacag accgattatg
     1741 aggcctggct tgcgaagttc ttcgagaccg taaaaccgca tctcgcggaa ggggcgccat
     1801 tatacgtctg gaacggtcat cgccagttcg gtccgatgca cgcaattctg accaatctcg
     1861 gatttcatgt atccaacgtg ataacttggg tgaagccttc cgcttcaccc ggctatgcgg
     1921 attacaagat gcagtctgaa ttctgcctct atgcgtggct tgaagggaac gggtcccatc
     1981 attggtttgg cggaaactca gaaagtaatg tgtgggagtg cccccgtgat gctgcccaca
     2041 ccttaatcca tccaacgaca aagcccgtag ctttggctca gcgagcgata aagaacagct
     2101 cacagcgagg ggatttgatt ctggattgct ttgccggatc cggaagtacg atcatggcag
     2161 cgcagagcat ggaacgggtt tgttgcgcta tggaactcga gcccgcttat tgcgacgcca
     2221 ttgtccggag atttataaag aaatatggac atgaaagcat aagcgccgag attcg
//
LOCUS       DAOSIO010000222         2270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_41072_length_2270_cov_2.305643, whole genome shotgun sequence.
ACCESSION   DAOSIO010000222 DAOSIO010000000
VERSION     DAOSIO010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41072_length_2270_cov_2.305643"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PK590_07350"
     CDS             complement(<1..440)
                     /locus_tag="PK590_07350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG24449.1"
                     /translation="MNILFYLQQFPAFGGIENVTATLARSFSEVGHSVIILSHAEKPA
                     AEICASLPDLVRQLKMPDQQHVVSTLNKSFLKETIEKNRVEVVIFQDSYARLDGNLIG
                     TLGSCRLIVCEHNSPHHVFCRANAKTLTFREVLGRIKYPFSHRL"
     gene            complement(437..1540)
                     /locus_tag="PK590_07355"
     CDS             complement(437..1540)
                     /locus_tag="PK590_07355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOG24450.1"
                     /translation="MKTGIITFHRAFNFGAVLQAYALQRTLERLGFTTEIVDYGSVGE
                     GSRFPNVRLTLKSVISFIKCLLCTAGADDLKRKRYRQFRKQYLVVSKSRYRDSEALKD
                     ADYACYVVGSDQVWNPVFSKAHPGYFLDFTEGRVISKKIAYAPSFGLSELPDEWVDYY
                     RSCLFKFDYISCREDEGQRIIKKLLGRSCPVLLDPTLLLTCHDYDKIAKESGKKTKYI
                     CCYTVGASRWTSVIAKKIAKKRGLRIKYLCSGARASWFGNQKFSSAGPSEFLGIVKNA
                     SLVVTNSFHGSVFSVIYQRPFFVCKNGTEGDSRLLTFLNTIGLVDRLIDPQNTCDEDF
                     QERPINYSLCNVLLSIEREKSLSYLQEALGIAK"
     gene            complement(1551..>2270)
                     /locus_tag="PK590_07360"
     CDS             complement(1551..>2270)
                     /locus_tag="PK590_07360"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOG24451.1"
                     /translation="LFSGTPCQVAGLRAFMGGEHRYLLCCDLVCHGVPSPLLFKMYLD
                     YLEQQKKQKIVAYSFRDKRGGWKRLGVSSVYSDNSESFCGMFDDSYGLAFLKDLAFRR
                     SCYRCRYAQKVRCGDITIADFWGVHRRYPHYDKDDKGTSLVLVNSRKGGDFLKKCDGK
                     LFLGPANLEDALQYNPRLEHPCPMPRERTTFYSDLVRCGFRQVIVKYHLHPPSFICRT
                     FQRASRGVRRAKVYFSGSVDR"
BASE COUNT          691 a          556 c          431 g          592 t
ORIGIN      
        1 attaaacgat gagaaaatgg gtattttatt cggcccagta cttctcgaaa tgtaagtgtc
       61 tttgcgtttg ccctgcaaaa tacatggtgc ggcgaattat gctcgcagac tatcaaccga
      121 caactgccaa gagtgccgat taagttgcca tccaaccgtg cgtacgaatc ttgaaagatg
      181 acaacttcca cacgattttt ttcaattgtt tctttaagaa acgatttgtt caacgttgaa
      241 acaacatgtt gctgatcagg cattttcagt tgccggacca gatcaggcaa cgaagcgcag
      301 atttcggctg ccggtttttc ggcatgagat aggatgatta cagaatggcc cacctcagaa
      361 aaactgcgcg ccaaagttgc cgtcacgttt tctatgccgc cgaaagccgg gaattgctgc
      421 aagtaaaaca ggatgttcat ttcgctattc ccaacgcttc ctgcaaatag gaaagtgatt
      481 tctcacgctc tatactgagg agcacattac acaatgaata attaataggc cgttcttgaa
      541 aatcctcatc acatgtattt tggggatcga tcaaacgatc aaccaacccg attgtgttta
      601 aaaaagtaag taagcgcgaa tctccctcgg ttccattctt acacacaaaa aacggacgtt
      661 gatagataac ggaaaacacc gagccatgga aggaattggt aactaccaag gaagcatttt
      721 ttacgattcc cagaaactca gatggcccag cagaactaaa cttttgattg ccaaaccacg
      781 aagcgcgcgc ccctgaacac agatatttga tccttaaacc cctttttttt gcgatttttt
      841 tagcaattac gcttgtccac cgactcgccc caactgtgta acaacaaata tattttgttt
      901 ttttgccaga ttctttcgca attttatcat aatcgtgaca agtcaataat aaagtcggat
      961 ccagcaagac tggacaagac cttcccaaca gcttttttat gatacgttgt ccttcgtctt
     1021 cacggcagga aatataatcg aatttgaaca aacaacttcg ataataatct acccattcat
     1081 cgggaagctc tgacagtccg aaacttggcg cgtaagctat tttttttgat attacgcgcc
     1141 cctctgtaaa atcgagaaaa tatccgggat gtgccttgct aaaaactgga ttccacacct
     1201 ggtcacttcc gacaacataa caggcataat cagcatcctt taaggcttcg gaatccctat
     1261 accggctctt cgatacaaca agatattgct tccgaaactg acggtaacgt tttcttttta
     1321 aatcatcagc acccgcagta cataacaaac actttatgaa agagattact gacttaagcg
     1381 tcaacctcac attagggaac ctgctcccct ccccaactga tccataatcc acgatttcag
     1441 tagtgaaacc caatcgttca agcgtacgtt gcaaagcgta agcttgcagc acggcaccga
     1501 aattgaaagc tctgtggaaa gttatgatgc ctgttttcat gtaactcaat ttatcgatca
     1561 accgatcctg aaaaataaac cttcgcgcga cgcacacctc gactggccct ttgaaaagtt
     1621 cgacaaataa acgacggtgg atgcaaatgg tatttaacaa tgacttgccg aaacccgcaa
     1681 cggaccaaat cggaataaaa cgttgtccgt tcgcgaggca ttgggcaggg atgctccaac
     1741 ctggggttgt actgtaaagc atcctctaag tttgccgggc ctaaaaacaa tttaccatca
     1801 cattttttta aaaaatcgcc cccttttcga gaattgacta acaccaacga ggtgccctta
     1861 tcatccttgt catagtgcgg ataacgacga tggactcccc aaaaatcggc aatcgtaata
     1921 tcgccacaac gaaccttctg cgcataacgg catcgataac aagaacgtct aaacgcaagg
     1981 tcttttaaaa atgctaagcc ataagagtca tcgaacatac cgcaaaagct ctcagagttg
     2041 tcactataaa cagatgacac tcccagcctt ttccagccac cacgtttgtc tctgaacgaa
     2101 tacgcaacta ttttttgttt tttttgttgc tcgaggtaat ctagatacat tttaaataaa
     2161 agcggcgaag gtacgccatg gcataccaga tcgcaacaca acagatacct atgctctccg
     2221 cccatgaagg cacgcaaacc cgcaacctga catggtgtcc ctgaaaagag
//
LOCUS       DAOSIO010000223         2268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_41101_length_2268_cov_5.298690, whole genome shotgun sequence.
ACCESSION   DAOSIO010000223 DAOSIO010000000
VERSION     DAOSIO010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41101_length_2268_cov_5.298690"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PK590_07365"
     CDS             complement(<1..223)
                     /locus_tag="PK590_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOG24452.1"
                     /translation="MTAKEIKYSTEARQKVLLGVDTLANAVKVTLGPKGRNVVISKTW
                     GTPLITKDGVTVAKEIELEDKFENMGAQMV"
     gene            complement(237..527)
                     /gene="groES"
                     /locus_tag="PK590_07370"
     CDS             complement(237..527)
                     /gene="groES"
                     /locus_tag="PK590_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOG24453.1"
                     /translation="MNIKPLNNRIIVSRLESEEKTPGGIIIPDTAREKPQEGKVLAVG
                     PGRRDKDGNRIALEVKEGDKVLFTKWGGTEIKIDDREIMMMKEDDILAVLES"
     gene            complement(661..1155)
                     /locus_tag="PK590_07375"
     CDS             complement(661..1155)
                     /locus_tag="PK590_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOG24454.1"
                     /translation="MIKNLLPAVRRKNDKALSRAEEHPFLPIQREMNRLFGDFFRDFG
                     LDPFGRVREFGFEPSVDVWEDDKNIHIKAELPGLDEKDIDVTLTPDALSIRGEKKEEK
                     EEKENGYWHKETSYGSFYRSIALPEGINQEKVEAKFKNGVLSIEIPWKEGTRAGKKVA
                     IKTS"
     gene            1401..1946
                     /locus_tag="PK590_07380"
     CDS             1401..1946
                     /locus_tag="PK590_07380"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:1617..1619,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOG24455.1"
                     /translation="MKQNRLVVFVLALVALTACANADIRNDAIPAGQKASDFTLPDQD
                     GKMWTLAETLKDYKAVVLAFYPKDDTGAUIRQFVEFQQNIGKYEARKIKILGLSRDSA
                     ESHRKFITKHKLYNMTLLVDEKGKIAKRYDADHWILPLSKRVYLVIDRNLNVLYRKDM
                     GFDLLPDQTQTLIREIDAKIP"
     gene            2084..>2268
                     /locus_tag="PK590_07385"
     CDS             2084..>2268
                     /locus_tag="PK590_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007420216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent alcohol dehydrogenase"
                     /protein_id="HOG24456.1"
                     /translation="MKNFKFYNPTKIIFGEGTIKEIAGEIPREARVLLTYGGGSIKRN
                     GLIRTSFLCRGVYLAVS"
BASE COUNT          543 a          587 c          527 g          611 t
ORIGIN      
        1 tgaccatctg cgcgcccatg ttttcaaatt tatcttccag ttcgatttcc ttggcgacgg
       61 tcacgccgtc cttggtgatc aggggcgttc cccatgtttt cgagatgaca acattgcgtc
      121 ccttcggccc cagcgtgact ttcaccgcgt tggccagcgt atccacgccc agcagaactt
      181 tctgccgggc ttcggttgaa tatttgattt cttttgccgt catgaaaaaa acctccttac
      241 gattcaagta ccgccagaat atcgtcttcc ttcatcatca tgatttcccg gtcatcgatt
      301 ttgatttccg ttccgcccca tttggtaaag agaaccttat cgccttcctt tacttccagc
      361 gcgatccggt tgccgtcctt gtcgcggcgg ccgggtccca cggccagcac ctttccttcc
      421 tgcggctttt ccctggccgt atccggaatg ataatgccgc cgggcgtttt ttcttcactt
      481 tccagacggg aaacaatgat ccggttatta agcggtttaa tgttcatttt ttccaacctc
      541 cataaaaatt aataaatccc gcctgccggg caggcgtgag aaacaatgtt tgaataaggg
      601 tctgtgctga atgctcaaga aaaaagggag tttcccgcaa ggggaaactc cttggtttac
      661 ttatgatgtt ttaatggcaa cctttttccc ggcccttgtg ccttccttcc aagggatctc
      721 gatactgaga acgccgtttt taaactttgc ctccactttt tcctgattga tgccttcggg
      781 aagagcgatg cttctgtaga aggagccgta actggtttct ttgtgccagt agccattctc
      841 tttttcctcc ttttcttcct tcttttcacc ccggatcgaa agagcatccg gcgtgagtgt
      901 cacatcaata tccttctcat cgagaccggg cagttcggct ttgatatgaa tatttttgtc
      961 gtcttcccag acatcgacgc tgggctcaaa accgaattcc cgaacacgac caaaaggatc
     1021 caggccgaaa tccctgaaga aatcgccaaa aagccggttc atttcccgtt gaatcggcaa
     1081 gaatggatgc tcttcagctc ggcttagggc tttatcgttt tttcgcctta cggcgggcaa
     1141 cagattttta atcatattcc ttcctccttt ccagcgaatt gtaatatatt aatattatta
     1201 acttattttt ctattatacg ccaaaaataa tcattaaaaa aaacttgtca aggggggggg
     1261 tgcaggaccg gcaaattccg cctctttgcg ggcgtcgtgt ttgacggaac gtaccgggca
     1321 gggatataat ggtttttaag gagcaatagc gcgttgggcg ttgtcatttt agaaacccga
     1381 tatgtggaaa ggagaggttt atgaaacaga acagacttgt cgtttttgtg cttgcgcttg
     1441 tggcacttac cgcctgcgcg aatgcggaca tcagaaacga cgcgatcccg gccggtcaga
     1501 aagcctcgga ctttacgctg cccgatcagg acggcaaaat gtggacgctg gccgaaacgc
     1561 tcaaagacta taaagccgtg gtgctggcct tttatcccaa agacgacacc ggcgcctgaa
     1621 ttcgccagtt tgttgagttt caacaaaaca tcggaaaata cgaagccaga aaaataaaaa
     1681 tcctgggact ctcgcgcgat tcggccgaat cgcaccgcaa gttcatcacc aagcacaagc
     1741 tgtacaacat gacgctgctc gtcgatgaaa agggcaaaat cgccaaacgc tatgacgcgg
     1801 accactggat tctccccctg tcgaagcgcg tttacctggt catcgaccgg aatctgaacg
     1861 tgctttaccg caaagacatg ggttttgacc ttctccccga tcagacgcaa acgctgatcc
     1921 gggaaatcga cgccaaaatt ccctagaaaa aggggttgcg tttattgctc tttgaattta
     1981 ttaatttccc aaacagaaca gccggaccat cgttgtgctg ccgtggccgc cggatgctga
     2041 tccgcgccgg ccccgggaaa ttcatcttgc atgagggggg accatgaaaa atttcaaatt
     2101 ttacaatccg acaaaaatca tcttcggcga gggaacgatc aaggaaatcg ccggagaaat
     2161 tcccagggag gccagggttc tgctgaccta cggcggcgga agcatcaaac gcaacggatt
     2221 gataagaact tcctttttgt gtcgtggagt atatttagcc gtctcttg
//
LOCUS       DAOSIO010000224         2236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_41929_length_2236_cov_3.825309, whole genome shotgun sequence.
ACCESSION   DAOSIO010000224 DAOSIO010000000
VERSION     DAOSIO010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41929_length_2236_cov_3.825309"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PK590_07390"
     CDS             <1..357
                     /locus_tag="PK590_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOG24457.1"
                     /translation="PLAAAMALAVGIGIQNFPEGLAVSLPLHRDGFSKKKSFLYGWLS
                     GIVEPAAAVIGALIVVVVQPLLPFALAFAAGAMIFVVVEEVIPESQQNGNGDLATTGT
                     ILGFALMMLLDVAFGK"
     gene            468..1346
                     /locus_tag="PK590_07395"
     CDS             468..1346
                     /locus_tag="PK590_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOG24458.1"
                     /translation="MILLLIIAAVAFLVFIAGIRIIRPTHRGLIERLGKYDRFANPGF
                     HWIIPVVEMMHMVNTTEQMVDADPQEIITNDNLNARVDAQVYFKVRQDEESVKNSQYN
                     VDNYQWQIVNLARTTLRNIIGTLTLKSANSERGKINLELHKTLLEETRSWGIDIVRTE
                     LKEIDPPKDVQETMNKVVKAENEKISAVDFATAAETVADGEKRASIKKAEGVKQAKIL
                     QAEGEAEAIRLVNEAANKYFVGNAQIFRKLQAVENALEHNAKIVLPSNAELINVIGDM
                     AGITPIPVKNRPQSTV"
BASE COUNT          625 a          563 c          498 g          550 t
ORIGIN      
        1 ccgctcgcgg ccgcgatggc gcttgccgtg ggtatcggaa ttcagaattt cccggaaggc
       61 ctcgccgtct ccctccctct tcaccgagac ggtttttcaa aaaagaaaag ttttctttac
      121 ggatggctgt ccgggatcgt ggaaccggcc gcggccgtta tcggcgcgct catcgtggtc
      181 gtcgtccaac cgcttctgcc gttcgctctc gccttcgccg cgggggccat gattttcgtc
      241 gtcgtggaag aggtgatccc cgagtcccag cagaacggca acggcgatct cgcgacgacc
      301 ggcaccatcc tcggctttgc cctcatgatg ctcctcgatg ttgccttcgg gaaataaatc
      361 caccctacgc aatttcaaat ttgcttctct cccaaagtcc ccaagtgcta taatttcggg
      421 ccagtttcat tgatcgcgtt tcaacccatc acgaggagga gaaaatcatg attttgcttc
      481 tcattatcgc cgccgtcgct tttcttgttt ttatcgcggg tatccgaatc attcgcccga
      541 ctcaccgtgg attaattgaa agactcggga agtacgaccg tttcgcaaat cccggttttc
      601 actggatcat tcccgttgtt gaaatgatgc acatggtcaa cacgacggaa cagatggttg
      661 atgccgaccc ccaagagatc atcacaaacg ataatcttaa cgcccgcgtc gacgcgcagg
      721 tttatttcaa ggtcagacag gatgaggaga gcgtcaaaaa ctcccaatac aacgtcgata
      781 actaccagtg gcagatcgtc aaccttgccc gtacgacctt aagaaacatc atcggcacgc
      841 tcacgctcaa atccgccaac agcgaacgcg gaaagatcaa tctcgaactt cacaaaaccc
      901 tgctcgagga aacaagaagc tggggcattg atatcgtgcg taccgagctt aaagaaattg
      961 accctccgaa ggacgtccag gaaaccatga acaaggtcgt gaaggccgag aatgaaaaga
     1021 tctcggccgt ggacttcgcg acagccgcgg aaaccgtcgc ggacggagaa aaacgcgctt
     1081 ccattaaaaa agccgagggg gtcaagcagg ccaaaatttt gcaggcggaa ggagaggctg
     1141 aagcgatccg tctcgtgaac gaggccgcca ataaatattt cgtgggaaac gcgcaaatct
     1201 tcaggaaact gcaggccgtc gaaaacgccc ttgagcataa cgccaagatc gtgctaccgt
     1261 ccaacgccga actcatcaac gtaatcggcg acatggcggg tatcaccccg atcccggtaa
     1321 aaaaccgtcc tcagtcaaca gtttaggcgc tgaatttgtt ctgatcttgt cggctcaaaa
     1381 gcatccttga aacgcaggcc tggagtattt ctcctctata tgacgttgtg tgtttcacct
     1441 taaatggatt tataaaatta ctgtgaggtt tgttacggtt taaacctgac gagatagcag
     1501 gaccacccac aatttaaaaa ttagcaacgc ggagaatcat tttatgaatt ttactgttag
     1561 tttcctaagg ctcgctttct cgacgggttt gcaaaatctc atttttcccc atggattcag
     1621 atccttgccc ctgccccaac tggaaatggg aattctggcc ttccaacttt ggaagtattt
     1681 ctggcaacta ttcggcgcca atatcttatc gcaagccagg tatttaggtt gcttgacaat
     1741 gtctcccgaa gcaatatctg tcaattaaat tcaaatctac tggggccatg aactaaatga
     1801 gcgctgcttt ggagaaaaaa caagacgctg aatcaaaaat gaagatcgga ttaatactac
     1861 cggcaaatat atggggatgc ccatacacca acatctacac agaaatatta aaagaaaaca
     1921 aagttgattt tgatttaatt tcttggaata gagacggatc ggaaaataat acggggcttc
     1981 agtttgaaaa acagttctgt cgcaaaaaca gatattcgaa actaattcct tatctttcct
     2041 acatttcttt catcaaaaaa attatacgga aaaacaagca ccacaaattg attatacacg
     2101 gttcccagat agcgatcctg ctttcttctt ttttgcgaaa acactacaac aaacgatata
     2161 tatttgatac cgcgacataa caatcgatca gttatggtat tttaaaagga atcttcacgc
     2221 cgttttgaga ggtagc
//
LOCUS       DAOSIO010000225         2209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_42618_length_2209_cov_3.701486, whole genome shotgun sequence.
ACCESSION   DAOSIO010000225 DAOSIO010000000
VERSION     DAOSIO010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42618_length_2209_cov_3.701486"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PK590_07400"
     CDS             complement(<1..600)
                     /locus_tag="PK590_07400"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG24459.1"
                     /translation="MMKKSNPKQSRVKSGKQPSGLNVGKIVRELREEAGLSGAELCRR
                     SGGLDSKTLTAVEKGRIKNPSITTLEYLARGLGMTISGIFKRAELLQEKHFYIGNQKG
                     VSRTEFIARGVQLVSFTPFSREFFCGKVILETGKSFDHSLLGHKGVFFIQILVGQVEG
                     VLEGKKVFLKEGESLYSKGEMVFNLKNLHQRSAALLLVTV"
     gene            complement(597..>2209)
                     /locus_tag="PK590_07405"
     CDS             complement(597..>2209)
                     /locus_tag="PK590_07405"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOG24460.1"
                     /translation="HADQERGFFYHFLDLETGKRAGKSELSPIDTALFIAGAIFAAEY
                     YGDPGIRDLARRLYERVDFPWMTNQGKTFALAWSPESGFSRLRWDHFDESLLLYVLAI
                     GAPGKPLPPEVWKAIRRPVGSYRNYRLIQMSPLFTHQYPQIWLDLKNKNDGFADYFEN
                     SVRATLANKAFCEDQSGKYRTYAEGFWGLSASDGPAGYKAYGAPPGWSVAHDGTIAPT
                     ACGSSIVFTPEASVECLKRIRERYGDKMWGRYGFADAMNRDQNWASDRVIGIDQGALL
                     LMIENHRTGLVWKTMTRNEWIRAALSKVGFRDGSMELPWPDPPVYTAPYVENGIQVDA
                     MLRDWSSGEAIFLNPEDHVESGHFAGPKDLSAKIRMAWNERYLFFVAQVTDDDLIMRR
                     RGKDIWRDDLMEFYVDPEGDGFYWGSRSDYQFGFRAGAGDANVESWSWFQGGESPITE
                     GKAVAVSYTDAQGYIIEGALRWEALGIFPKPGMTVRLSPAVHDIDRKKGDAKLVWFFR
                     NEKEYQRFELGKVILSPSAKTGEAFQGE"
BASE COUNT          529 a          703 c          478 g          499 t
ORIGIN      
        1 gaccgtcaca agcagaagtg ccgcggatcg ctggtgcaaa tttttaagat taaaaaccat
       61 ttcccctttt gaataaagac tttctccctc tttcaaaaac actttcttcc cttccaggac
      121 gccctccacc tggcccacaa gtatctgtat aaaaaaaacg cccttatgtc ccagcaggga
      181 gtggtcaaaa cttttccccg tttcaaggat caccttgccg caaaaaaatt ctcgggaaaa
      241 aggagtaaac gataccaact gaacaccccg agcgataaat tccgtcctcg agaccccttt
      301 ctgatttccg atgtaaaaat gtttttcctg cagaagctcc gcccttttaa aaattccact
      361 gatggtcatt cccagccccc gggccagata ttccagcgtc gtgatcgagg gatttttgat
      421 tcgtcccttt tcaaccgccg ttagagtctt gctatcaagc cctccggatc gccggcaaag
      481 ctctgccccc gaaagtccag cctcctcgcg aagctccctc acaatttttc cgacattgag
      541 accagaaggc tgtttcccgc ttttcaccct ggattgcttc ggattacttt ttttcatcat
      601 tcaccctgaa aggcctcacc tgtttttgct gacggactca agatcacttt gccaagttca
      661 aatctctgat attccttttc gttacgaaaa aaccagacca gtttcgcgtc tccctttttt
      721 cggtcaatat catgaacggc aggcgacagc cggacagtca tcccgggttt cggaaagatc
      781 ccgagggcct cccaccggag agccccttca atgatgtagc cctgggcatc cgtgtaagaa
      841 accgccaccg ctttcccttc cgtgatgggc gattctcccc cctgaaacca ggaccagctc
      901 tcgacattcg cgtccccggc cccggcccgg aacccgaact gataatcact ccgcgaaccc
      961 cagtaaaaac catcgccttc cgggtccaca taaaactcca tcaaatcatc gcgccaaatg
     1021 tccttgccgc gccgcctcat aattaaatca tcatccgtca cctgcgcgac aaaaaaaagg
     1081 tatcgctcat tccacgccat ccggattttc gcggaaaggt cttttggccc ggcaaagtgg
     1141 cccgattcga cgtggtcttc aggattcaag aaaatcgcct cccccgagct ccagtcgcga
     1201 agcatcgcgt ccacctgaat tccattttcc acgtaaggcg cggtataaac cggaggatcc
     1261 ggccacggga gctccatact cccgtcccta aacccgactt tggatagcgc ggcgcgaatc
     1321 cattcatttc gcgtcatcgt tttccaaaca agaccggtcc tgtgattttc aatcataagc
     1381 aacaaggcgc cctggtcgat tccaatgacc cggtcactcg cccagttctg atccctgttc
     1441 atcgcgtccg cgaagccgta acggccccac atcttgtcac cgtagcgttc acggatccgc
     1501 ttcagacatt ccacggacgc ctcgggcgtg aacacgatcg aagaaccaca ggccgtcgga
     1561 gcaatcgttc cgtcgtgggc cacgctccat cccgggggcg ccccgtaagc cttgtaaccc
     1621 gccggcccgt cggaagcgct cagtccccaa aaaccttcgg cataagtccg gtacttcccc
     1681 gactgatctt cacaaaacgc cttgttggca agcgtcgcgc gaaccgagtt ttcaaaataa
     1741 tccgcgaacc cgtcattttt gtttttcaga tccagccaaa tctgcggata ctggtgcgta
     1801 aaaagcggcg acatctggat caaccggtaa ttccggtaac taccgacggg acggcgaatt
     1861 gccttccaga cttccggcgg caacggtttt cccggagcgc cgatcgcgag cacatacaaa
     1921 agaagcgact cgtcaaaatg gtcccaccgc agacggctaa acccgctttc gggagaccac
     1981 gcgagcgcga aggtctttcc ctgattcgtc atccacggaa aatcgactcg ctcgtaaaga
     2041 cgtctcgcca gatcacgaat tcccggatcc ccgtaatact cggcggcaaa aatggctccc
     2101 gcgatgaaaa gcgccgtatc aatcggagac agttcggatt ttcccgcgcg cttcccagtt
     2161 tccagatcga gaaaatgata aaaaaatccc cgctcctggt cagcgtgat
//
LOCUS       DAOSIO010000226         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_43191_length_2187_cov_4.072233, whole genome shotgun sequence.
ACCESSION   DAOSIO010000226 DAOSIO010000000
VERSION     DAOSIO010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43191_length_2187_cov_4.072233"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PK590_07410"
     CDS             complement(<1..290)
                     /locus_tag="PK590_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24461.1"
                     /translation="MGTPKSSFSKFRALDLPVILAGFVAIFFLLTNGVPLIQAGWVGE
                     LGKKSRYVRLMIVSVAVFSILMFCIRWRWPEIFKRLFWARLWHAVCQIPLRYL"
     gene            complement(436..1143)
                     /locus_tag="PK590_07415"
     CDS             complement(436..1143)
                     /locus_tag="PK590_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG24462.1"
                     /translation="MQKISIIIPVYNEARTILELVARVLDVSFPIGREILIIDDCSND
                     GTDKALEELSRRHAAEKIRIMRNEKNRGKGASVIRGLEAADGDIVVVQDADFEYSPSE
                     LPRLIEPILRGEADAVYGSRFQAGYPTGMAFPNYFANVFLTALTNILFGTRLTDMETC
                     YKLLRRDIIASLQLRADRFEFEPEITSKLALTKRRIIEMPISYEGRTAAQGKKIKARD
                     FFIALAVLFKNRFSKLE"
     gene            complement(1154..1948)
                     /locus_tag="PK590_07420"
     CDS             complement(1154..1948)
                     /locus_tag="PK590_07420"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOG24463.1"
                     /translation="MVESIRELNKICQKPNYRTVGNWYVRNILRDAALPMTWLLLHTG
                     VSANQVTFFSFLVASLGGLSYVIPGAGPFLVSALLLQFWYYLDHVDGQIARYRKTASM
                     TGRFLDFMTHHLVHLVLFFALGYYAYKALAREIFLLWGSVSSLAILAFNLIHDVKYKT
                     FYERCCRDGAVIKKSSQGSKKTEQPKSFWRKAFSLAHKSCEIHVMINVLAGAALLQFV
                     TVWDLRVLLFFYYGTVAPVVFGVKFFYIICSQAIDTEYGCFTCQKS"
     gene            complement(1941..>2187)
                     /locus_tag="PK590_07425"
     CDS             complement(1941..>2187)
                     /locus_tag="PK590_07425"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24464.1"
                     /translation="NQALKEILNGLSIEHIDLLPAFIEENKKIKEKFYFDTDPHWTTA
                     GHAVAARTIADYLLKRSFASPGSSFVPIGQDAGGENG"
BASE COUNT          646 a          541 c          480 g          520 t
ORIGIN      
        1 agataccgga gcggaatttg gcaaacggca tgccacaacc gggcccagaa aagacgtttg
       61 aagatctccg gccatcgcca gcggatacaa aacatcagga tcgaaaaaac cgcgactgaa
      121 acgatcatca accggacata gcggctcttc tttccaagtt ctcccaccca tcccgcctgg
      181 atcaacggga ccccgtttgt cagcagaaaa aagatcgcaa caaaacctgc caaaatgacc
      241 gggaggtcaa gtgctcgaaa tttcgaaaaa ctgcttttgg gggttcccat aacgtcacaa
      301 cacctgtttt tttaccggat gccggccggc gggagacgtc agataaactt agtaaatcaa
      361 tttcccgcat caggaagaat ttgattctcc atccgatatc agattatgac ctttccctcg
      421 gacggcgccg ggcaactatt cgagtttaga aaagcgattt ttaaagagca cggccaatgc
      481 aataaaaaaa tcgcgcgcct ttatcttttt cccctgagca gcggttctcc cctcataact
      541 gatcggcatt tcgataatcc gtctcttggt tagggcaagc tttgacgtaa tctccggctc
      601 aaactcaaag cggtctgccc ttaattgtag cgatgcaata atgtcgcgcc gtaaaagctt
      661 ataacaggtt tccatgtcgg ttaaccgggt gccgaaaagg atattggtca acgccgtcag
      721 aaaaacattt gcgaaataat tggggaatgc catgcccgtg ggatagccgg cttgaaaacg
      781 gctaccgtaa accgcatcgg cctctcctct taaaatcggt tctattaagc gaggtaattc
      841 cgatggagaa tattcaaaat cagcgtcctg aacaacgaca atatctccgt ctgcggcctc
      901 aagtccccga atgacgctcg ctcctttccc ccgatttttc tcattccgca taatcctgat
      961 cttctcagcg gcatgccgcc gggataattc ttccagagcc ttatctgtgc cgtcgttgct
     1021 gcaatcgtcg atgataagaa tctcgcgtcc gattggaaaa gaaacatcga gaaccctggc
     1081 aacaagctcc aaaattgtgc gtgcttcatt gtaaacagga atgataatag aaatcttttg
     1141 cattggagtc ctttcaggat ttttgacagg tgaaacatcc gtattcagta tcaatcgctt
     1201 gggagcatat aatataaaaa aacttgaccc caaagaccac aggcgcaacc gttccataat
     1261 agaaaaaaag caaaacgcgg aggtcccaaa ccgtcacaaa ctggagcaag gcagccccgg
     1321 caaggacatt gatcataaca tggatttcgc acgatttatg ggcaagagaa aaagctttcc
     1381 tccagaaact tttcggctgc tcagtttttt ttgaaccttg agaagatttt ttaattacag
     1441 caccgtcccg gcagcacctt tcataaaaag ttttatactt tacgtcatgg ataagattaa
     1501 acgcgagaat tgcaagcgaa ctgacggatc cccaaaggag aaagatctca cgagccaagg
     1561 ctttataagc gtaataacca agcgcgaaga aaaggaccag atgaacgagg tggtgggtca
     1621 taaaatcaag aaaacggccg gtcatggatg ccgttttccg ataacgcgcg atctggccgt
     1681 caacatggtc aaggtagtac cagaattgga gcagcagggc actcacaaga aaagggccgg
     1741 ccccgggaat gacataagaa agtcctccca atgacgcaac aagaaacgag aaaaatgtta
     1801 cttgattagc agaaaccccg gtgtgtaaaa gcaaccaggt cattggcaat gccgcgtctc
     1861 tcaagatgtt ccgcacatac caattcccga ccgttcggta attcggtttt tgacaaatct
     1921 tgttgagttc gcggatagat tcaaccattt tcaccccctg catcctgtcc gatgggcaca
     1981 aaagatgatc ccggactagc gaaacttcgc ttcaataaat aatcggctat cgtgcgggcc
     2041 gcaacggcat gtcctgctgt ggtccaatga ggatcagtat caaaataaaa tttttctttt
     2101 atcttcttgt tttcttcaat aaacgcagga agaagatcga tgtgctcgat actcaaccca
     2161 tttaaaatct ccttcagagc ttgattc
//
LOCUS       DAOSIO010000227         2183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_43313_length_2183_cov_4.981673, whole genome shotgun sequence.
ACCESSION   DAOSIO010000227 DAOSIO010000000
VERSION     DAOSIO010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43313_length_2183_cov_4.981673"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PK590_07430"
     CDS             <1..199
                     /locus_tag="PK590_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24465.1"
                     /translation="KPYHYDTKTPGWEARITTELNQLWEHWIRLHPDQWLWGHRRWKD
                     KPRTIRTVSQQTISTGRADEH"
     gene            189..1352
                     /locus_tag="PK590_07435"
     CDS             189..1352
                     /locus_tag="PK590_07435"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOG24466.1"
                     /translation="MNIELTIEKLVFGGDGLGFVDGKAYFVEGALPGEKVLARITNDK
                     KNYGKARLIKVLSPSPERLTPPCPYVQSCGGCQYQHLRYTDELRWKELQVRESFAQTI
                     RIDPALIQPIRQGPREYHYRQSITLHRTAKNNQKPSPLAFIGRDNRSFVFIQNCLLAG
                     ESLQSVFKELHTLGKYETKRVFKIAENGAIVSSEEDKLYRIRLADELFWAHSRGFFQN
                     NLEVTQQVVEELQNIVEMVKPSRFIDLYSGVGTFAALAGSQAQEIFCFEESIYAVECL
                     KRNLKERQLPGAIFKGRVEQTFPNFWLGNQKEGSLVLLDPPRLGITYDLASFFAKADK
                     MKNIIYLACDLQILIRDLKIILSKGRYQIKTIVPFDMFPRTKHLEVLAWLEQI"
     gene            complement(1362..>2183)
                     /locus_tag="PK590_07440"
     CDS             complement(1362..>2183)
                     /locus_tag="PK590_07440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HOG24467.1"
                     /translation="EDRRSGDSDSLYFEVVSSGGLSVRGKAIILCGGGKSYPALGADG
                     NAYGLAKKFGHSTVEPVPSTVPLIVKDPWCHFLQGQKIRAKVRALEGGEVLREADGDL
                     LFTQYGLSGTAILDVSDPVSIALNREKKKEMILEMDLLSFLSEDELRSEIKKRVRRGI
                     PSGDLLEGLLPHKFSTLLAREADVLTGNVSGASLDLLVAQIKRKKFRVLGTRGWNEAE
                     FTAGGIPSFEVTEELGSKFQRGLAFAGEIVDVQASRGGYQLAWAWASGAVAGSAA"
BASE COUNT          561 a          629 c          487 g          506 t
ORIGIN      
        1 aaaaccctac cattacgaca ccaagacccc aggttgggaa gcgcgtatca ccacagagtt
       61 aaaccagctt tgggagcact ggatccgcct ccatcccgat caatggctgt gggggcatcg
      121 tcgctggaaa gacaagccaa gaacaattcg aaccgtttca cagcaaacta tttctactgg
      181 ccgtgctgat gaacattgag cttacgatcg aaaaactggt ctttggcggt gacgggttag
      241 gctttgttga cggaaaggcc tatttcgttg aaggcgcgct tcccggagaa aaagtgcttg
      301 cccgaattac caatgataaa aaaaactacg gtaaagcgcg gctgatcaaa gttctgagcc
      361 cttcccccga gcgcctcacg cccccttgcc cttatgtcca atcttgcggc ggttgccagt
      421 atcagcacct ccgctacacc gatgaactcc ggtggaagga attacaggtt cgggaaagtt
      481 ttgcgcaaac aattcgcatc gaccccgctt taattcagcc gatccgtcaa ggccccagag
      541 aatatcatta tcgccaaagc atcacgctcc accggaccgc gaaaaacaac cagaagccga
      601 gtcctcttgc ttttattgga cgggataacc gctcctttgt cttcatccaa aattgccttc
      661 tcgcgggcga atcccttcaa agcgttttta aggaattgca caccctcggc aaatatgaaa
      721 ccaagcgagt tttcaaaatc gcggaaaatg gtgcgatcgt ctccagcgaa gaagataagc
      781 tgtaccggat caggttggcg gacgaacttt tctgggctca ctcaagaggc tttttccaaa
      841 acaatcttga ggtcacgcaa caagtcgtcg aagagctaca aaacatcgtc gaaatggtca
      901 aaccttcccg ttttatcgac ctttactcgg gagtggggac cttcgcggcg ctcgcgggtt
      961 cccaagcgca ggaaatcttc tgttttgagg aaagtattta cgccgtggaa tgtctcaaaa
     1021 ggaacttgaa agaaagacag ctccctggcg caattttcaa gggcagggtg gaacaaactt
     1081 ttccgaattt ctggctcggg aatcaaaaag aaggttcact cgttcttctg gacccgccgc
     1141 ggctcggaat cacttacgat ctggccagtt ttttcgccaa agcagacaag atgaaaaata
     1201 tcatctatct cgcgtgcgat ctccagatcc tcatccggga tctcaaaatc atcctgtcaa
     1261 aaggccgtta ccagattaaa accatcgtgc cgtttgacat gttcccccgg accaaacatc
     1321 ttgaagtgct ggcctggctt gagcaaattt aaccgttttc ttcaggcggc agacccggcg
     1381 acagcgccac tggcccaggc ccaagccaac tgataccctc cgcgcgaagc ctgaacatcc
     1441 acaatttctc ccgcaaacgc aagcccccgt tggaactttg atcccagttc ttcagtcact
     1501 tcaaacgaag gtattccgcc agccgtaaac tccgcttcat tccagccccg ggtccccagc
     1561 accctgaatt ttttacgctt aatctgcgcc accagcaggt ccagagacgc cccggagaca
     1621 ttcccggtta aaacgtcggc ctccctggcc aacagcgtgg agaatttatg cggcaagaga
     1681 ccttccaaaa gatcccccga cgggatgccc cgtcttaccc tttttttaat ctccgatctc
     1741 agttcatcct cacttaaaaa tgagaggaga tccatttcaa gaatcatttc tttctttttt
     1801 tcccggttca gcgctatcga aaccggatcg ctgacgtcca gaatcgcggt gccggacaat
     1861 ccatactgcg taaaaagaag gtctccgtcc gcttctctca aaacttcccc tccctcaagc
     1921 gcccttactt ttgcccgaat tttctgtcct tgcagaaaat ggcaccaggg atctttaacg
     1981 atcaggggaa cagtgctggg aaccggttca accgtcgaat gcccgaattt tttggcaaga
     2041 ccgtacgcgt tcccatcagc tccgagcgcg ggatacgatt ttcctcctcc gcaaagaatg
     2101 atggccttcc cgcgaacact gagcccgcct gatgaaacca cttcaaaata aagagaatcc
     2161 gaatctccgg atcgtctatc ttc
//
LOCUS       DAOSIO010000228         2179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_43435_length_2179_cov_3.362053, whole genome shotgun sequence.
ACCESSION   DAOSIO010000228 DAOSIO010000000
VERSION     DAOSIO010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43435_length_2179_cov_3.362053"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..639
                     /gene="rpsE"
                     /locus_tag="PK590_07445"
     CDS             154..639
                     /gene="rpsE"
                     /locus_tag="PK590_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOG24468.1"
                     /translation="MDSTTFEKVIQVNRCAKVVKGGKRFSFSALVIVGNGAGEIGFAQ
                     GKANEVADAIKKALVAARKNKIKITLKGSTIPHEVIGRYGAAKVLLKPAAPGTGVIAG
                     GSVRAACEACGIKDILTKSLGSDNSINVLKASVDGLTSLVDRKVRMDEVEEEKKVEHA
                     A"
     gene            629..1060
                     /gene="rplO"
                     /locus_tag="PK590_07450"
     CDS             629..1060
                     /gene="rplO"
                     /locus_tag="PK590_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOG24469.1"
                     /translation="MRHKTPHKKRSKRLGRGIGSGHGKTSTRGHKGQRSRSGYRFRPG
                     FEGGQNPLYRRLPKRGFNNNEFKTSYTVVNLKQIAVLDEKEITPEILRARGVVKKLQD
                     GLKVLGMGDLKSAVTVKAHSFSASAKSKIEAQGGQAVLLKK"
     gene            1129..>2179
                     /gene="secY"
                     /locus_tag="PK590_07455"
     CDS             1129..>2179
                     /gene="secY"
                     /locus_tag="PK590_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOG24470.1"
                     /translation="MLQAFGNILKVPDLRKKIFFTFMIIAIYRIGAYVPTPGVDGVAL
                     ARFFRQNAGGGNLLDFMGMFTGGALQKATIFALGIMPYISASIILQLLTVVIPALEKM
                     AKEGEEGRKKIIQLTRYGTIILSILQTFFISLWLENPAAFNGTVIVPNPGWGFRLMCM
                     LTMTTGTAFIMWLGEQIDAHGIGNGMSIIITAGIIEALPEALRQTWILLSPLDPSRQQ
                     LPWWKFGIMCLLFVGAVLFLILIIQGQRKIPVQYAKQIRGHRVYGGQSTYLPLKVNQA
                     GVIPIIFAQSVILFPATLAGFFPNAGMLTQVAKLLAYGTVTYSLIYALGIIFFTFFYT
                     AITFNPVDVSDNLQKY"
BASE COUNT          553 a          473 c          596 g          557 t
ORIGIN      
        1 cagcgatcgt ctacattggt gacggttaat ttcaagaaag gaactaattt aagcatgcaa
       61 aaggaagcaa cgcccgaaac tcacgaaaaa aaggaagggg cccctcatca aaagcccggc
      121 aaggcggtca ttgcctccac gtcgcggcag ggaatggatt caacgacttt tgaaaaagtc
      181 atacaggtta accgttgcgc caaggttgtt aaggggggga aacggtttag tttcagcgct
      241 cttgtgatcg tgggaaacgg agctggcgaa attggttttg cccagggaaa ggctaatgaa
      301 gtagcggacg cgatcaagaa ggcccttgtc gccgcgcgta aaaataaaat taaaattacc
      361 ctcaagggaa gtactatccc gcatgaagtg atcggaagat atggtgccgc caaagtactt
      421 ttgaaaccgg cggctccggg gacgggagtg atcgcggggg gctcggtgcg tgcggcctgt
      481 gaggcctgcg ggatcaagga tattttgacg aaaagtcttg gcagtgataa ttcaatcaat
      541 gtgttgaagg cttcggtgga tggattgact tcgcttgtgg accggaaagt tcgtatggat
      601 gaagttgaag aagaaaagaa ggttgaacat gcggcataaa actcctcata aaaaaagatc
      661 aaaacgcctg ggccgtggta ttggttcagg gcatgggaaa acgtccacca ggggccacaa
      721 ggggcaacgg tcgcggtcgg gctatcggtt tcgtcccggg tttgaagggg gacagaaccc
      781 cttgtaccgg cgtctcccca agcggggttt taataataac gaatttaaga catcctatac
      841 ggttgtaaat ttgaagcaga tagcggtgct tgatgagaaa gaaattacgc cggaaattct
      901 gcgcgctcga ggcgtggtta aaaaacttca ggatggtctt aaggttctcg gtatgggcga
      961 tctgaagtcg gccgtaacgg tcaaggctca tagttttagc gcttccgcca aaagtaaaat
     1021 tgaggcccag ggcggtcaag cggttctgct taaaaagtaa tataaattcc cctctgcaac
     1081 ttcacattgt gaagcaaagc ttgaggtcat tataagaacg gagttctggt gctacaagcg
     1141 ttcggtaata tccttaaagt ccccgatctt cgaaagaaga tttttttcac gttcatgatc
     1201 atcgcgatct accggatcgg cgcctatgtg ccgacccccg gtgtcgacgg tgtggcgttg
     1261 gcaagatttt tccgtcaaaa cgcgggcggt ggtaacctgc tcgattttat gggaatgttc
     1321 accggcggag cgctccaaaa ggcaaccatt tttgcgcttg gaattatgcc gtatatctcg
     1381 gcatccatta ttttacagct tctgaccgtg gtaattcccg cgcttgagaa aatggccaag
     1441 gagggcgaag aaggtcgaaa aaaaatcatt cagttgaccc gctacggcac gattattctg
     1501 agtattttac aaactttttt tatttctctt tggcttgaaa atcccgcggc attcaatgga
     1561 acggtgattg ttccgaatcc cggatggggc ttccgtctta tgtgtatgct gaccatgacg
     1621 acagggaccg cttttatcat gtggctcggc gagcaaatcg acgctcacgg aattggcaac
     1681 gggatgtcga ttattatcac ggcgggtatt attgaagcgc ttcccgaagc tttgcgtcag
     1741 acttggatcc tgttgtcgcc tctagatcct tcccggcaac aattaccgtg gtggaaattt
     1801 ggtatcatgt gtttgctctt tgtcggagcg gttctctttt tgatcctgat tattcagggg
     1861 cagaggaaga tcccggttca gtacgcgaaa cagatacgag gccacagggt atacgggggg
     1921 caaagtacgt atctaccgct gaaggttaat caggcgggcg tgatcccgat cattttcgcg
     1981 caatcggtca tcctttttcc ggccacgctg gcggggtttt tcccgaacgc gggaatgttg
     2041 actcaggtcg ccaagctttt agcttacgga acggtcacct attctttaat ctacgctctt
     2101 ggaatcatct tttttacgtt tttttacacg gcgatcacct ttaatcccgt ggatgtaagt
     2161 gacaatcttc aaaaatatg
//
LOCUS       DAOSIO010000229         2149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_44254_length_2149_cov_3.121299, whole genome shotgun sequence.
ACCESSION   DAOSIO010000229 DAOSIO010000000
VERSION     DAOSIO010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44254_length_2149_cov_3.121299"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..1608)
                     /locus_tag="PK590_07460"
     CDS             complement(109..1608)
                     /locus_tag="PK590_07460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOG24471.1"
                     /translation="MESILHYLYQYVKFGKFTTVFLVVIGAVIVLKLLLKPRLMIPKR
                     ALLISILLLGLFLRIGWLSYSAHTPMTEWKTGHMLENDWINVHAIELAEHGTWFHGPN
                     GLPSGRRPIGYPMFLALFYKILGPRLAVAWSLNLLLYGVTILFLFGIARRLFDDNVAL
                     ITALLFAIYPMSIYSIKLLTDEHLFLPVWYGGLYLLIRMLDGRKVPLDWLWLGIIFGY
                     ATMIRTHPIFMPFVVGLAFWLMKRPVKEVVAKAILVALVMNLVNLPWVIRNYRAWGVP
                     VLYTATGSFIYAQVNSTATPEGGGHIPLEGEPDYSPELDTAIKSGNEGVIHQISNRLM
                     KQWIFQHPRQFLELGPSRLLDFMCFNRKSGVWAIWHQYYPGSFDPEKPLPENLRQFFE
                     ESAFAFYYVLFFSWILGMILLVSRWKTIPVVTQMGLLLLGSCFLFWFMEHMVIYPDRK
                     YRFPLEPLMLISASYFFLYFSCPAVIHTIIKEISKKRQLKKQILPDAGN"
     gene            complement(1608..1919)
                     /locus_tag="PK590_07465"
     CDS             complement(1608..1919)
                     /locus_tag="PK590_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24472.1"
                     /translation="MMATKVRSVDERPLSFKQLKEFGSVFTSFRHREFWLFSLFIFLH
                     FFLVRRWHPSEVRYWKKVIEVGEEYRDIFSRLQRVDDFCLRHFSWLRYLCWNTVVEVV
                     K"
     gene            complement(1942..>2149)
                     /locus_tag="PK590_07470"
     CDS             complement(1942..>2149)
                     /locus_tag="PK590_07470"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG24473.1"
                     /translation="IAERLAEKFGVQPEFTEASSGALPYPAATFDYVFGNGVLHHVEL
                     SSTAGEVRRVLKPGGGLFCRAVAL"
BASE COUNT          681 a          550 c          465 g          453 t
ORIGIN      
        1 caagcactcg aaatttcgaa aaactacttt tgggggtttc cataacgtca caatatccgt
       61 ttttttaccg gatgccatcg gttggagacg ccaagcgaat ttagcaaatc aattccccgc
      121 gtcaggaaga atttgcttct tcaattgccg ttttttagat atttctttta tgatcgtatg
      181 tattactgcg gggcaagaaa aatagagaaa aaaatacgac gcagagatta acatcagggg
      241 ctctaacgga aacctgtact ttcgatccgg ataaataacc atatgttcca taaaccagaa
      301 gagaaaacag gatcctaata aaagaagccc catttgcgtc acgacaggta tcgttttcca
      361 tctgctgacc aagaggatca tccccaaaat ccaggaaaag aagagaacgt aataaaacgc
      421 aaaggcactt tcttcaaaaa attgacggag gttctccggc aatggctttt caggatcaaa
      481 ggaacccggg tagtattggt gccagatcgc ccaaactcca cttttgcgat tgaaacacat
      541 aaaatccagc aaccgggaag gccccaattc caggaactga cgaggatgct gaaaaatcca
      601 ttgtttcatc aatcgattag agatctggtg gattacccct tcgttacccg actttatggc
      661 agtatcaagt tccggagaat agtccggttc cccttcaagg ggaatatgcc ctccgccctc
      721 cggtgtcgcg gttgaattaa cctgcgcata aataaaagac ccagtcgccg tgtagagcac
      781 tgggactccc caagccctat aatttcgaat cacccagggt aaattgacca gattcatgac
      841 cagggcaacc aggatcgctt tcgcgacaac ttctttcacg ggccgtttca tcagccagaa
      901 tgccaaccct acaacaaaag gcatgaaaat cggatgcgtc cgtatcatgg tggcatatcc
      961 gaaaatgatc ccaagccaca gccaatccag tggcaccttt cgtccatcca gcatgcgaat
     1021 caaaaggtag aggccgccgt accagacggg taaaaaaaga tgttcatccg tcagaagttt
     1081 aatgctataa atcgacatgg gataaatcgc gaacaaaagc gcggtaatca acgcgacatt
     1141 gtcatcaaaa agccgccttg caattccgaa gagaaaaagg atcgtcaccc catacaaaag
     1201 aaggttgagg gaccaagcaa ccgccaaccg aggccccagg attttataga acaaagccag
     1261 gaacatcggg tacccgatag ggcgacgacc ggagggtaaa ccgttggggc catgaaacca
     1321 cgtaccgtgt tcagccagtt cgatcgcgtg aacattaatc cagtcgtttt caagcatatg
     1381 gccagtcttc cactcggtca tcggggtatg tgcggaataa ctcaaccagc caattctaag
     1441 aaagaggcct aaaagaagga ttgaaatcaa cagcgcacgt ttgggaatca tcaaacgggg
     1501 ttttagcaaa agcttcaaca caatcaccgc cccgattacc acgagaaaaa cggtcgtgaa
     1561 tttcccaaac ttcacatatt gataaagata atgcaggatc gattccatta cttaaccacc
     1621 tccaccacgg tattccagca aagatacctc agccacgaaa aatgacgcag acaaaaatcg
     1681 tcaacacgct gaagcctcga aaagatgtcc ctatattctt cccccacttc gataactttc
     1741 ttccaatagc gaacctcgga aggatgccac cgccgaacga gaaagaagtg aagaaaaata
     1801 aaaagagaaa aaagccagaa ctcccggtgc cggaacgaag tgaaaaccga accaaactct
     1861 ttaagctgtt taaagcttaa gggccgctca tccactgacc ggaccttcgt tgccatcatc
     1921 cggtagacat ggattgccgg gttataaggc aacggctcga caaaaaagcc ccccccccgg
     1981 ttttaacacg cgcctgactt ctccagccgt cgacgagagc tcgacatggt gaagcacccc
     2041 atttccaaaa acatagtcaa aggtcgcggc cggataagga agtgcccctg aagacgcttc
     2101 ggtaaattcg ggctgaacgc caaatttttc agccaaacgt tccgcgatc
//
LOCUS       DAOSIO010000230         2118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_45147_length_2118_cov_3.421716, whole genome shotgun sequence.
ACCESSION   DAOSIO010000230 DAOSIO010000000
VERSION     DAOSIO010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45147_length_2118_cov_3.421716"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..936)
                     /locus_tag="PK590_07475"
     CDS             complement(199..936)
                     /locus_tag="PK590_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24474.1"
                     /translation="MISALIKLIFNLIRWAVILSVVALIFHTWTIRQVVKHTLSWQLG
                     APVEIGSVKMDWKNTGFELHGLEIGNPYHYGSGLMADIPLVIVSLDATKIPAGVFRFR
                     TVGLYLRKLNIIHSPGNGFNVMELKTIKKQSPSVHKNIAAADPEHLKDNAQVAIDEFI
                     ISIGDITLVEALTGLQKEKHIKANIQGQTFYNVSVPQDLVYVTVMLAMKKIGYNFAED
                     QLRQFLGASVIAPAKTSLFEKIKKSFA"
     gene            1273..>2118
                     /locus_tag="PK590_07480"
     CDS             1273..>2118
                     /locus_tag="PK590_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOG24475.1"
                     /translation="MKDFEDKIVLITGGTGSFGTALLKKFIHTGIMDQLREIRIFSRD
                     ELKQDMMRNEFNIKKIKYYIGDVRNRASVDQAMQNVNFVFHAAALKQVPSCEFFPLEA
                     IQTNVLGSSNVIRSAIANEAEKVIMLSTDKAVYPINVMGMSKALMEKLVQAESRLKHV
                     KTVVCAVRYGNVMCSRGSVIPLFIQQIKNNSPITVTAPEMTRFMLSLDDAVNLVIFAF
                     LNAQHGDVFIKKAPACTVGDLVDVLKKIFHSTTPVKVIGIRQGEKIHETLASSMEMIR
                     SKDWGD"
BASE COUNT          599 a          523 c          436 g          560 t
ORIGIN      
        1 agccgtcaag ttttttttta cactttttta aggggctatt cccatccctc gaccagcgcg
       61 gacgtttcca caccccagga acatgccgat ttcctatcca agcctcacga tagaaaagga
      121 ttcgccgaaa cacggcttaa taactttcaa cctagaggaa tcctccctca aacaaaaatg
      181 taattttctg tgcaactcct acgcgaaaga tttcttaatt ttctcaaaca agcttgtttt
      241 ggccggagca atcacgctgg cccccaaaaa ctggcgcaac tgatcctcgg caaaattgta
      301 gcctattttt ttcatggcaa gcatgacggt cacgtaaacc agatcctgcg ggacagacac
      361 attgtaaaaa gtttgtccct ggatattcgc cttgatgtgt ttttctttct gcaaaccagt
      421 cagggcctca accagcgtaa tgtccccgat tgaaataatg aactcgtcaa tcgcaacctg
      481 ggcattgtct tttaaatgct ccggatcggc cgcggcgata tttttatgaa cggaaggtga
      541 ctgcttttta atggttttta actccattac attaaaacca ttccccggag agtgtatgat
      601 attaagtttg cgaagataca atccaacggt tctaaaccgg aaaactcccg cgggaatttt
      661 tgtggcatcc agcgaaacaa tcaccagagg gatgtccgcc atcagccccg agccgtaatg
      721 gtaggggttt ccaatctcaa gcccgtggag ctcaaatccc gtatttttcc aatccatctt
      781 aacggaacct atctcaaccg gagctcccaa ctgccaactc aaggtgtgtt tcaccacttg
      841 acgaatcgtc caagtatgaa aaatcagggc gaccacggaa aggatcactg cccatcgtat
      901 gaggttaaat ataagcttga tcaatgcgct aatcatggtc gccccttggc tttcatgtat
      961 cggcactctc aaaaatgtct tgaaatctcc tacgtctctt tcttctgcaa aaattcattt
     1021 cgtgttcaat ctgagtttcc acagggcaaa agaacccgca ccctattttt taccgctact
     1081 ccaaaagaac actatttcat atgtaccctg ccgccctttg cgccaatctt tgcctttgct
     1141 ttgctacact tctttgctac attattcatc aacgcaagtc tgcactgaaa tgactcagcc
     1201 ggatctctat attttaggtg aagaactttt gttccccaaa atgtgttttt acaatgacta
     1261 cgagggtaaa ccatgaaaga cttcgaagat aagattgttc tgataacagg cgggacaggc
     1321 tctttcggaa ccgcactgct aaaaaaattt attcatacag gcatcatgga tcagcttcgg
     1381 gaaattcgca tttttagcag agacgagtta aagcaagata tgatgcgtaa tgaatttaat
     1441 atcaaaaaaa tcaaatatta cattggcgac gtaaggaatc gagccagcgt ggatcaggca
     1501 atgcaaaatg ttaatttcgt atttcacgcg gccgccctga agcaagtacc ctcttgtgaa
     1561 tttttcccgc tggaagcaat tcagaccaat gtcctgggaa gttcaaacgt catccgatcc
     1621 gcaatcgcca acgaagcgga aaaggtgatt atgctaagca ccgacaaggc ggtctatccc
     1681 atcaacgtta tgggaatgag caaagcattg atggaaaagc tggtgcaagc agagtcccga
     1741 ttaaaacatg ttaaaacggt cgtctgcgcc gtgcgatatg gcaacgtcat gtgttcacgg
     1801 ggatcggtaa tcccactttt catccaacag attaaaaaca acagccccat caccgtcaca
     1861 gcccccgaga tgactcgctt tatgctctcc ctagacgatg cggtcaatct cgttattttc
     1921 gcgtttctca atgcgcaaca tggcgatgtc ttcattaaaa aggcgcccgc ctgcacggtg
     1981 ggagatctgg tcgacgtatt aaaaaagatt ttccattcca ccactcctgt caaagtaatc
     2041 gggataagac aaggggaaaa gatccatgaa acgctggctt cgtccatgga aatgatacgc
     2101 tccaaggatt ggggggat
//
LOCUS       DAOSIO010000231         2113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_45292_length_2113_cov_4.037415, whole genome shotgun sequence.
ACCESSION   DAOSIO010000231 DAOSIO010000000
VERSION     DAOSIO010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45292_length_2113_cov_4.037415"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /locus_tag="PK590_07485"
     CDS             <1..1456
                     /locus_tag="PK590_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015175292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG24476.1"
                     /translation="RMKVYDPKHLEKAPEGFKSADARMPVFKGQKFTIQIAPEDCTGC
                     GVCVETCPAKSKTDPSKKAINMQPQIPIRESEKPKWDYFLNIPDFDRAKLNPGLVNQQ
                     QAMRPLFEFSGACAGCGETPYVKLVSQLFGDRAVIANATGCSSIYGGNMPTTPWAKDA
                     NGRGPAWANSLFEDNAEFGFGFRMAIDKQKEFAEELLKKLEGKIGADQVSALLHAPQK
                     TEGEIQAQRERVADLKKKLATLQPDPDAGQLLSLADTLVKRSVWIMGGDGWAYDIGFG
                     GLDHVIASGRNVNILVLDTEVYSNTGGQMSKSTPRSAVAKFAMAGKSAGKKNLGLIAM
                     TYGNVYVASIALGANDQQALKAILEAEAYDGPSLIIAYSHCIAHGINMTKGLQEQKAA
                     VASGQWVLYRFDPSKLEKGENPLVIDSKAPSIPVEQFLLAENRFKMLTKSKPERAKEL
                     FKLAQRDVDYRWKLFEYLAQGKAQPDAPTSGSAS"
     gene            1499..>2113
                     /locus_tag="PK590_07490"
     CDS             1499..>2113
                     /locus_tag="PK590_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOG24477.1"
                     /translation="MKPDTVASVPPKKDLKALFEPQTIAVVGASRRPEAVGYAITRNL
                     LSTGYKGKIYLVNPKADEIMGMKCYPSVADIPGVIDLAMFIIPAAGVPAELEACAQKG
                     AKAAIVISAGFREVGPEGLKLEKQVQKIAEQYGVAVLGPNCLGLINNDPEFSMNASFA
                     GTIPMPGNVAFVSQSGALGTALLDYARGENIGFSKFVSLGNKVGV"
BASE COUNT          504 a          511 c          632 g          466 t
ORIGIN      
        1 tcggatgaag gtttatgatc cgaagcatct tgagaaggcg cctgaaggtt ttaaatccgc
       61 ggacgccagg atgcccgttt ttaaagggca aaagtttacc atccagatcg cgccggaaga
      121 ctgcacgggt tgcggtgttt gcgtcgagac gtgcccggcg aaaagcaaaa cggatccctc
      181 caagaaggcg atcaatatgc aaccgcaaat tccgattcgt gaatccgaaa agccgaaatg
      241 ggactatttc ctcaacattc cggatttcga tcgcgcgaaa ctgaacccgg ggcttgtgaa
      301 tcagcaacag gcgatgcgtc cgttgtttga attttcggga gcgtgtgccg gttgcggcga
      361 gactccttac gtcaagctcg tgtcccagct ttttggtgac cgcgcggtga tcgccaacgc
      421 gaccggttgc tcgtcgattt atggcgggaa tatgcccaca actccgtggg cgaaggacgc
      481 gaacggcagg ggacccgctt gggccaattc tctttttgag gataacgcgg aatttggttt
      541 tggtttccgc atggcgatcg ataaacaaaa ggagttcgcg gaagagcttc tcaaaaagct
      601 cgaagggaaa attggcgcgg accaggttag tgccttgctc cacgcccccc agaaaaccga
      661 gggtgaaatc caggcgcaga gagaacgtgt cgccgatctc aagaagaagc tcgcgacgct
      721 tcagcctgac ccggacgcgg ggcagttgct ttcgctggcg gacacgctcg tcaaaaggag
      781 tgtctggatt atgggtggcg atggctgggc ctatgatatc ggttttggcg gactggatca
      841 cgtgatcgcg tccgggcgga acgtgaatat tcttgtcctc gataccgagg tgtattccaa
      901 caccggcgga cagatgtcaa aatcaactcc gcgttcggcc gtcgcgaagt ttgcgatggc
      961 cggaaagtcg gccggcaaga aaaatcttgg tctgatcgcg atgacttacg ggaacgttta
     1021 cgtggcctcc atcgctctcg gggcgaatga tcaacaggcg cttaaggcga tcctcgaggc
     1081 cgaagcttac gatggaccgt ctctgattat cgcgtacagt cattgcatcg ctcacggaat
     1141 taatatgacc aagggcttgc aggagcagaa agccgccgtg gcgtccggtc agtgggtgct
     1201 ttaccggttc gatccgtcca aactggaaaa aggggagaat ccgcttgtga tcgattcgaa
     1261 agctccctca atacccgtgg aacaattttt gttggccgag aaccgtttca aaatgctgac
     1321 gaaaagcaag ccggaacgcg cgaaagagct tttcaagctt gctcagaggg acgtggatta
     1381 ccgatggaaa ctttttgagt atcttgctca aggcaaggct cagccggatg cgccgacgag
     1441 cggttctgca tcctagacaa acagctgttc aacgatccca gatgataagg aatacattat
     1501 gaaaccagat accgttgctt ccgtgccccc taaaaaagat ctgaaagcgt tgttcgaacc
     1561 acagacgatc gctgtggttg gagcctcccg gcggccggaa gcggttgggt acgcgatcac
     1621 ccgaaacctt ttgagcacgg gttataaagg aaagatctat ctggtgaatc cgaaagccga
     1681 cgagattatg ggaatgaagt gttacccgtc cgtggcggat attcccggag tgatcgatct
     1741 ggcgatgttt attattccgg cggccggtgt ccccgctgaa cttgaggcgt gcgcccaaaa
     1801 aggggccaaa gcggcgatcg ttatttccgc ggggtttcgg gaagtggggc ctgaagggtt
     1861 gaagcttgaa aaacaggttc agaaaatagc ggaacaatat ggcgtggctg tgctggggcc
     1921 gaattgcctc ggtctcatta ataacgatcc ggagttcagt atgaacgcca gctttgccgg
     1981 aaccatcccg atgcccggaa atgtcgcttt tgtctctcag agtggcgcgc tcgggacggc
     2041 gttgcttgat tacgcccggg gagaaaacat agggttttcg aagttcgtaa gtttggggaa
     2101 taaagtcggc gtg
//
LOCUS       DAOSIO010000232         2087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_46115_length_2087_cov_3.603346, whole genome shotgun sequence.
ACCESSION   DAOSIO010000232 DAOSIO010000000
VERSION     DAOSIO010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46115_length_2087_cov_3.603346"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PK590_07495"
     CDS             complement(<1..221)
                     /locus_tag="PK590_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24478.1"
                     /translation="MRGIFFFALGLGAIVYLILILSGFSAMTLRNLTLVLILPILIRI
                     GSLRTFIQWIREILRYLFSELFRFHRGSV"
     gene            complement(320..>2087)
                     /locus_tag="PK590_07500"
     CDS             complement(320..>2087)
                     /locus_tag="PK590_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24479.1"
                     /translation="LTWFVFGIYTAIRMLILSAIRSRITAGFFIVGVAALVLSLPQIC
                     LTFQLAILSGRSGLEEGYAYVGSLSPPALLTIVFPNLQGLFRGNCIYSGIFAIFFTVA
                     AFFASDKNIQRIRLLWLWMAALSLLLALGQWSPLYVALIKLTQFYSFRVPAKFIVFFC
                     FSFAMLAGIGVQVILKDLPTDDLKRKVFAKRYLILAAGILVLWGGSYLTLTRGRALLV
                     QVGKYVVENFIYGKPGRPRTLESYMDSIYGTVENATSLLSLVDPWQIWAVFLILASCI
                     LACLFMTRVNVRIFFMTTLIMLVVDLYVFAGADIKTDFDSFRNVQRLSPVAKAVLEKK
                     QAGTLHRIYGYRRGDNGQLMPPSANMIYGTEDIGAYSPLVMKRYFETIGQLGCVNDSN
                     QMLDPDPKFILERLPLLDALDVSHVLSTEVMEHPALKLEFHDPASSARLYRNTGDYRR
                     CFFVPLQGVEFAEWPAIRNRFMSAGFDPQKTLLLEASDRKKIPQTKLSEANASLIQAA
                     IIEQRNEKETWFVKTTGPGFFVVTNMMYPGWNAALDGEPVPILSAYGLFRAVYLANAG
                     EHRIDFAYSPFNSLKKSFATWA"
BASE COUNT          664 a          529 c          464 g          430 t
ORIGIN      
        1 atcaccgacc cgcgatgaaa gcggaaaagt tccgaaaaga gataacggag tatctcccga
       61 atccactgaa taaacgtcct caatgaaccg atcctgatca aaatcggcag aatgagaacc
      121 agggttagat tccggagcgt catggccgaa aaaccactca ggatcaggat tagataaact
      181 atagccccaa gccccagggc gaagaaaaag attccgcgca aaaaaaacgg gtatgttttt
      241 aggagcggtg caaaataact acccaggagg aaagtcgatg gaaagaaaag gacccagaat
      301 ccaatcgcaa tcaacaattc taagcccatg ttgcaaaact cttcttcaga gaattgaacg
      361 gagagtaggc aaaatcaatc cggtgctccc ccgcattcgc aaggtagacg gcacgaaaga
      421 ggccgtaggc gcttaaaatc gggacaggct ctccgtcaag agcagcattc catcctggat
      481 acatcatatt ggtaacgaca aaaaaaccgg gacccgtcgt tttgacgaac caggtttctt
      541 tttcatttct ttgttcgatt atcgctgcct ggatcaatga tgcatttgcc tcactaagtt
      601 ttgtctgagg aatctttttt cgatctgaag cttccagcag aagagttttt tgcgggtcaa
      661 atccggcgct catgaaccgg ttcctgatag ccggccattc agcaaattca acaccttgca
      721 gcggaacaaa aaaacatcgc ctgtaatccc ctgtatttcg atagagacgc gcagatgacg
      781 ccggatcgtg aaattcgagc ttgagagcgg gatgctccat tacctcggtt gaaagaacat
      841 gggagacatc cagcgcatcc agaagaggaa gccgctccag gataaacttc ggatcagggt
      901 ccagcatctg atttgaatcg ttgacacagc ccaactggcc gattgtctca aaataacgtt
      961 tcatgacaag cggtgaatag gcacctatgt cttcagtccc ataaatcatg tttgcggagg
     1021 ggggcatcag ctgaccgtta tcccctcgcc gatacccata aatacgatgg agagtccctg
     1081 cctgtttttt ctccaagacc gcctttgcga cgggcgacag ccgttgaaca tttcggaagc
     1141 tatcaaaatc cgttttaata tcggcccctg caaacacata aagatcgaca accaacatga
     1201 tcaatgtagt cataaaaaat atccggacat tgacgcgagt cataaataag catgcaagaa
     1261 tacagcttgc gaggatcaga aaaactgccc atatctgcca agggtcaaca agagacaaca
     1321 atgatgtcgc attttcaaca gttccataaa tgctatccat gtatgactcc aaggtcctcg
     1381 gacgacccgg ttttccataa ataaaatttt ctactacata cttgccgacc tgtacgagca
     1441 aggcgcgtcc tcgcgtcaaa gttaaataac tcccccccca caaaaccaga attcccgccg
     1501 caagaatcag gtagcgttta gcaaaaacct tccttttaag atcatccgta ggcaaatcct
     1561 tcaaaatcac ctgaaccccg ataccggcaa gcattgcaaa actaaaacaa aaaaagacaa
     1621 taaacttcgc tggaacccgg aaagaataga attgggtcag cttgataagg gccacataga
     1681 gggggctcca ctggcctagc gccagcagaa gcgacaaggc cgccatccaa agccatagaa
     1741 gtctgattcg ctggatattc ttgtccgatg caaagaatgc ggccaccgta aaaaaaattg
     1801 caaaaatccc ggaataaata caattccctc ggaaaagccc ctgtaaattc gggaagacaa
     1861 tggtcagaag ggcgggcggc gacagagacc ccacatacgc atacccctct tcgagacctg
     1921 agcggccgga caatatggca agctgaaacg tgaggcaaat ctgaggaagc gacaaaacaa
     1981 gagccgcaac ccccacaatg aaaaatcccg cggttatcct gctgcggatt gccgaaagaa
     2041 tcagcatccg aattgccgtg taaattccga aaacaaacca ggttagc
//
LOCUS       DAOSIO010000233         2049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_47274_length_2049_cov_3.379137, whole genome shotgun sequence.
ACCESSION   DAOSIO010000233 DAOSIO010000000
VERSION     DAOSIO010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47274_length_2049_cov_3.379137"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PK590_07505"
     CDS             complement(<1..244)
                     /locus_tag="PK590_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOG24480.1"
                     /translation="MKITEFLDIKGIKLELEATDKEGVLREMVDLLTEIKEVGDQKAI
                     LKALIERENLGSTGIGQGIAIPHGKTDKVDGLAAVLG"
     gene            complement(276..830)
                     /gene="raiA"
                     /locus_tag="PK590_07510"
     CDS             complement(276..830)
                     /gene="raiA"
                     /locus_tag="PK590_07510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOG24481.1"
                     /translation="MDIRFTGQNLKITAGMRGHLETRLPKLEKYVPKIIEAHVFLKKV
                     KYLFVAEITVLAKDVRAFGKGSSKENVFASMDDAYDRVVKQLKKHRGREKNHHQKKGK
                     STLRAVRAAEAMVESELALGSKRPRIVSTEDFAVKPMSPEEASLQLEVSGESFLVFLN
                     PVTNSVNVLYQRKDGDHGLIEPQF"
     gene            complement(872..1597)
                     /gene="lptB"
                     /locus_tag="PK590_07515"
     CDS             complement(872..1597)
                     /gene="lptB"
                     /locus_tag="PK590_07515"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOG24482.1"
                     /translation="MNLLETRKLVKSYKGRTVVKGVNIQISRGEIVGLLGPNGAGKTT
                     TFYMVVGVIRPDEGGIFFRGEDITKLPMFERARLGIGYLSQEPSVFRKLTVEENIMAI
                     LETLEISQEERDRRLKRLLNDLDIAYLAKNKAYTLSGGERRRLEITRALVTNPSLILL
                     DEPFSGVDPIAVFEVQKILQRLKAKGLSILLTDHSVRETLSITDRSYLLYEGKVEVSG
                     TSGFLANDAKAREIYLGERFSLA"
     gene            complement(1601..>2049)
                     /locus_tag="PK590_07520"
     CDS             complement(1601..>2049)
                     /locus_tag="PK590_07520"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOG24483.1"
                     /translation="VKKDNINVEGLGARADTQLKKVKFKKNVTVIVQSSEEEGSGPTT
                     ITCDGPLVVDYDKNIAHFKENVVTQDSRGKLTANMMDVYYNRVSRRVSKIVAVGDVVI
                     ENPDGNKTFSDSVIYLAEEGRIILGGDTEALYYGGEGPEGLEKGIF"
BASE COUNT          485 a          585 c          421 g          558 t
ORIGIN      
        1 tcccaagtac cgcggctaat ccatcaactt tatccgtctt gccatgtggg atagcaatcc
       61 cctgtccgat ccccgtggag ccaaggtttt cgcgctcgat caaagctttc aaaatagcct
      121 tctgatcgcc cacttccttg atctccgtga gaagatcgac catctcgcgg agaacacctt
      181 ccttgtccgt ggcctcaagc tcaagcttga tccctttgat atcaagaaac tctgtgatct
      241 tcatgcccat atcctcctta cgtaaaagta tgttgttaga attgcggttc aataagtccg
      301 tgatcgccgt ccttccgctg gtaaagaacg ttgacactat tcgtcacggg attcaaaaaa
      361 accaggaacg attcgcccga gacctcaagc tgtaaactag cttcttccgg cgacatcggt
      421 ttcacggcaa aatcttcggt gctaacgatc ctcggccgtt tgcttccgag agccaattcc
      481 gactccacca ttgcctcggc cgcgcgaacc gcgcgcaagg tgctttttcc ttttttctgg
      541 tgatgattct tttcccggcc ccggtgtttt ttaagctgtt ttacaacccg gtcataagca
      601 tcatccatgg acgcgaaaac attttctttt gacgaaccct ttccaaacgc gcgcacatct
      661 ttggcaagca ccgtaatctc ggcgacaaaa aggtatttca cttttttaag aaaaacgtgc
      721 gcctcgataa ttttcggcac gtacttttcg agcttgggaa gccgcgtttc aagatgtcct
      781 ctcatgcccg ccgtgatttt aagattttga cccgtgaaac gaatatccac taataacctc
      841 cttgatggta atagaaagga atgtttctaa attacgccag tgaaaacctt tcacccaaat
      901 aaatctctct cgctttggcg tcattggcaa gaaaccctga ggttcccgaa acctcgacct
      961 tgccctcata gagcaagtac gaacgatcgg taatcgaaag agtttcccgt acgctgtggt
     1021 cggtgagaag aatgctgaga cctttcgcct tcagcctttg aagaattttc tgaacttcaa
     1081 aaacggcgat cgggtccact ccgctgaacg gctcgtccaa caaaatcaga gacgggttgg
     1141 tgacaagcgc ccgcgtaatc tccagccggc gacgttcgcc gccggataag gtataagctt
     1201 tgtttttggc aaggtaggca atgtccagat catttaatag cctcttaaga cgccggtcgc
     1261 gctcttcctg cgaaatttcc agggtctcga ggatcgccat gatattttct tcaacggtca
     1321 attttcgaaa aaccgacggc tcctgggaaa gatacccgat cccgagtcgg gcccgctcaa
     1381 acatgggaag cttcgtgata tcttctcccc taaaaaaaat ccccccttcg tcgggacgaa
     1441 tgacacccac caccatataa aaggtcgtcg tctttcccgc tccattcgga ccgagaagcc
     1501 ccacgatctc tccccgggag atctggatgt taacaccctt gaccaccgtg cgccctttgt
     1561 aacttttaac cagttttctt gtttcaagaa gattcatggc ttaaaaaatt cctttctcca
     1621 gtccctccgg accttcacca ccgtagtaga gagcttccgt atccccgccc agaataattc
     1681 ttccttcttc cgcaagataa atcacactgt ccgaaaacgt cttgtttccg tccggatttt
     1741 cgatcaccac gtctcccacc gcgacaatct ttgagaccct gcggcttacc cggttatagt
     1801 agacatccat catgtttgcc gtgagtttcc ctcgactatc ctgcgtcacg acgttttctt
     1861 taaaatgagc gatattttta tcgtaatcaa ccaccagcgg accgtcacag gtaatggtcg
     1921 tggggcccga accttcctcc tcggaacttt ggacgattac agtcacattt ttcttaaatt
     1981 tcaccttctt gagctgggtg tccgcgcggg cccctaaacc ttcaacattg atgttatctt
     2041 tcttgacct
//
LOCUS       DAOSIO010000234         2023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_48095_length_2023_cov_3.292683, whole genome shotgun sequence.
ACCESSION   DAOSIO010000234 DAOSIO010000000
VERSION     DAOSIO010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48095_length_2023_cov_3.292683"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PK590_07525"
     CDS             complement(<1..345)
                     /locus_tag="PK590_07525"
                     /inference="COORDINATES: protein motif:HMM:NF020013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24484.1"
                     /translation="MEHNLYQRKTCRYCDTPLGSAFLDLGLQPLANNLPRKDDAGKPE
                     FRCPLSLVKCPACALVQLTHSVPANLMFDEYLYVSSTTRTFRTHFAEYAEDVKSRIGS
                     KKTRLRWTLAAMT"
     gene            complement(351..1076)
                     /locus_tag="PK590_07530"
     CDS             complement(351..1076)
                     /locus_tag="PK590_07530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG24485.1"
                     /translation="MNKTVVITGASRGLGLELAKKFIAAGDTVFGISRTRKYWAVARL
                     FEPSRKFRLMQCDVASESAVASLVRKIKRTAPRVDILINNAGYCGRLMRVEETSVRDL
                     TKHYRQNLVSAFLMCKYFIPIMKTVARPLIVNISSMAGTRAVPRLFPYSAVKFGVLAL
                     SQAIAKENDGNLKCVTVCPGGMNTKMRADLFGREDSDRQQEAGFVAEVTDRVIRGEIE
                     VLSGGHIVIRHGKITGIYPPPAA"
     gene            complement(1085..1498)
                     /locus_tag="PK590_07535"
     CDS             complement(1085..1498)
                     /locus_tag="PK590_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG24486.1"
                     /translation="MELETIKHIDPAFVDARGEIFNIFEGNIGHVAYITSKKGSVRAN
                     HYHKKDIQYMYLISGAYETHCCPVEDPSRKQVLKVKAGDIVKTPPMVAHAQKFTEDSV
                     FLSLTTRMREKGKYEEDTIAFAVIDGYLNKELKTR"
     gene            complement(1619..>2023)
                     /locus_tag="PK590_07540"
     CDS             complement(1619..>2023)
                     /locus_tag="PK590_07540"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOG24487.1"
                     /translation="PDDYRVLPNFIEHALRKQPLPIHGDGRNTRSFCYINDGVEALFR
                     VLFSEANGEAFNLGNPEPEISIQELAHYVQKAMPYPVEIVNIDPPHAVYAKSDPKRRC
                     PDITKLSTVTGYKPRYGLEEGLKRTIAWFQEF"
BASE COUNT          473 a          523 c          516 g          511 t
ORIGIN      
        1 cgtcattgct gccaatgtcc accgcaagcg cgtttttttg gatccgattc gcgatttcac
       61 atcctcggca tattccgcga agtgcgtacg aaacgtccgg gtggtcgaac tcacgtagag
      121 atattcatca aacatcaggt tcgcgggaac ggaatgggtc agttgaacca gggcgcaagc
      181 cggacatttg acgagagaaa gaggacaacg aaattcaggc ttcccggcgt catccttgcg
      241 cgggaggttg ttggcaagcg gttgcaggcc aagatccaga aaggcgctcc ccagcggggt
      301 atcacagtac cggcacgttt ttcgctgata caggttatgt tccatagtct ctacgccgcc
      361 ggtggaggat aaatgccggt aatctttccg tgacggataa caatatgtcc gccggaaagc
      421 acttcgatct ctccccggat aacccgatcc gtcacttccg cgacaaaacc ggcctcctgc
      481 tggcgatccg agtcctctcg tccgaaaaga tccgcgcgca ttttggtatt cattcctccg
      541 ggacaaacag tcacgcattt gagattgcca tcgttttctt ttgcgatcgc ctgcgaaagg
      601 gcaagcactc caaacttgac ggcggagtac gggaaaagtc tcggtaccgc gcgcgtaccc
      661 gccatggaag aaatattgac aataagcggc cgggccacgg ttttcataat cggaatgaaa
      721 tacttgcaca tcaaaaacgc ggaaacaaga ttttgccgat aatgttttgt gagatccctt
      781 accgaagttt cctcgacacg cataagccgc ccgcagtagc cggcgttatt aatcaaaata
      841 tccacccggg gcgcggttct tttgattttt cgaaccaacg acgcgacagc gctctcggac
      901 gcgacgtcgc attgcataag ccggaatttt cgggaaggtt cgaaaagacg tgccacagcc
      961 cagtattttc gggtccggct gatcccgaaa acggtatctc cggccgcgat aaactttttt
     1021 gccagttcga gtcccagtcc gcggctggcg ccggtaataa caactgtttt gttcatgatg
     1081 gagtttatcg ggtttttaat tctttgttga ggtacccgtc gatcacggcg aaggcgatgg
     1141 tgtcttcttc atactttccc ttttcgcgca tccgtgtggt taacgagaga aaaacggaat
     1201 cttccgtgaa tttctgcgcg tgggcgacca tcggtggtgt tttaacgatg tcaccagcct
     1261 tcactttcag cacctgcttc ctagagggat cttcaaccgg acagcagtgg gtttcataag
     1321 ccccgctgat caggtacata tactggatgt cttttttatg ataatggttt gcccggacgg
     1381 accctttttt tgaggtgatg taggcaacgt gaccgatatt cccctcaaaa atgttgaaga
     1441 tttctccgcg ggcatcgaca aacgcggggt caatatgttt gatggtttct aattccatga
     1501 caggttcctt tctttcttgg attaacttca gcggagcaac gtcttacggg aaatcgggaa
     1561 atgtgaaaca aaacccttgg tctaaaaaac attctctttc ccccgtttcc cggtattttt
     1621 aaaactcctg aaaccaggct atcgtacgtt tcagcccctc ctcaaggccg taccggggct
     1681 tataaccggt caccgtggaa agtttggtaa tgtcagggca acggcgtttc gggtcactct
     1741 tggcatagac cgcgtggggc ggatcgatat tcacgatttc caccggatac ggcatagctt
     1801 tttggacgta atgcgccagt tcctgaattg agatttcggg ctcagggttt cccaggttga
     1861 aagcttcacc gttggcctcg ctgaagagga cacggaaaag cgcctcaacc ccatcattga
     1921 tgtaacaaaa agaccgcgtg ttccggccat ccccgtgaat cggcaacggt tgcttgcgca
     1981 gtgcatgctc aataaaattg ggtagcacac gataatcgtc cgg
//
LOCUS       DAOSIO010000235         1997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_48917_length_1997_cov_3.407827, whole genome shotgun sequence.
ACCESSION   DAOSIO010000235 DAOSIO010000000
VERSION     DAOSIO010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48917_length_1997_cov_3.407827"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="PK590_07545"
     CDS             complement(<1..924)
                     /locus_tag="PK590_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG24488.1"
                     /translation="MSSATAWIANRIKRDQIDKYLEGGRDFIQDEKIWDEINSAREPS
                     KERAREILAKSLEVQTLTPAELAELIQVRDPEMIREMRETALKIKLKVYDNRIVTFAP
                     LYMGSYCVNNCAYCGFKRENETARRRVLSMEEIRRETESLAGAIGHKRIIAVYGEHPL
                     TGIDYIAESMRTIYDVKVPLEKGHGNIRRINVNAAPMPVEELKLLHDVGLGTYQVFQE
                     TYHHETYARLHPKGTLKSDYQWRLYAMHRAMEPETGIDDVGIGVLFGLYDWRFEVMAL
                     LHHAIDLESKMGIGPHTVSFPRLEPALNAPYV"
     gene            complement(938..>1997)
                     /gene="hydF"
                     /locus_tag="PK590_07550"
     CDS             complement(938..>1997)
                     /gene="hydF"
                     /locus_tag="PK590_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOG24489.1"
                     /translation="RSDVTLLVLESGVWTPFEDEVVREAESRKSPLIVVINKSDVTPP
                     SAEFLTRVREKTSRFLISVSTDLSKRDAQVTELKRFLLEVCPDEYLSAPSLLGDLMPR
                     GGLAVLIVPIDAVAPKGRIILPQAQAIRDALDHHCMALVVKESEYRQALSKLAGLPDL
                     VVCDSQVVEQMVTGTPEKVKCTTFSILLSRLKADIVEMTRGALAVDRLKPGDRVLIAE
                     SCSHHAIGDDIGRVKIPAWLKKHLGHDIHIDVTSGRDFPPDVRKYQLVIQCGSCMLTR
                     RETLVRVQRAKLAGVPITNYGLCISYLRGVLGRVIEPFPEALKAYEDMRKQLPLRHTG
                     ARSEKDRNGESRGTKERS"
BASE COUNT          481 a          589 c          468 g          459 t
ORIGIN      
        1 aacatagggg gcgttcaacg ccggttccag tcgggggaag gaaaccgtat gcggtccaat
       61 ccccattttc gactcaagat caatcgcatg atgaagtaac gccatcactt caaaccgcca
      121 gtcataaagg ccaaagagga ctccaattcc aacatcgtca atccccgtct caggttccat
      181 ggcccgatgc atggcataaa gccgccactg ataatccgac ttcagagtac ccttcgggtg
      241 aagccgggcg taggtctcat gatgatacgt ttcctggaac acctgatagg tcccgaggcc
      301 cacatcatgc aacaacttca actcctcgac gggcatgggc gcggcgttga cgttaatccg
      361 gcggatattt ccatgccctt tttcaagcgg caccttcacg tcataaatcg ttctcatgct
      421 ttcggcgatg taatcaattc cggtcagggg atgctcgccg taaaccgcga tgattcgttt
      481 atgcccgatg gcgcccgcca gagattccgt ttcccggcgt atttcttcca ttgagaggac
      541 acgtctccta gcggtctcat tttcacgctt aaaaccgcaa taagcgcaat tattcacgca
      601 gtaactgccc atataaagcg gcgcgaaagt cacgatcctg ttgtcataga ccttcaattt
      661 gatcttgaga gccgtttccc gcatttcgcg gatcatctca ggatcacgaa cctggatcag
      721 ctcggccaat tcggccgggg tcagggtctg cacctccagg gatttggcca aaatctcacg
      781 cgcccgctcc ttcgagggtt cccgggccga attgatctca tcccagattt tctcatcctg
      841 aataaaatcc cggccccctt caagatactt atcgatctga tcacgtttga ttctgttcgc
      901 aatccatgcc gttgccgaac tcatgatagc ctccgtttta agacctctcc tttgtaccgc
      961 gcgattcccc gttccgatct ttttcgctcc gcgccccggt atgccgcaga ggtaattgct
     1021 ttcgcatatc ttcataagct ttcagagctt caggaaacgg ttcaataacc ctcccgagga
     1081 caccccgaag ataagaaata caaagtccgt aattggtgat cggaactccc gccagtttcg
     1141 ctctctggac acgaaccagg gtctcacgtc gcgtcagcat gcaagaaccg cattgaataa
     1201 caagctggta cttccggaca tccggcggga aatcccttcc tgaagtgaca tcgatatgga
     1261 tatcatgccc aagatgtttc ttgagccagg ccggaatttt gacgcgcccg atatcgtctc
     1321 cgatcgcgtg gtgactgcaa gattcagcga tcaagactct atccccaggc ttgaggcgat
     1381 ccaccgccaa agcgcctcgc gtcatttcca caatgtccgc cttgagcctc gagagcaaaa
     1441 tcgaaaaggt cgtgcatttc actttctcgg gggtcccagt caccatttgt tcaaccacct
     1501 gggaatcaca aacaacaaga tccggaagcc cggcaagttt tgaaagcgcc tgtcgatatt
     1561 cactttcctt gacgaccagc gccatacaat gatgatccag cgcgtcgcgg atcgcctgcg
     1621 cctggggcaa aataatcctt cccttcgggg ctacggcatc gatcggaacg atgagaaccg
     1681 cgagacctcc ccgcggcatc aaatctccga gaagcgacgg cgccgaaagg tattcgtccg
     1741 gacagacctc aagaagaaaa cgcttgagtt cggtcacctg cgcgtcacgc ttcgaaagat
     1801 ccgtgcttac agagataagg aagcgggacg ttttttcacg aacacgcgtc agaaattcgg
     1861 cggacggagg ggtaacgtca gacttgttaa tcaccacgat caatggactc tttcgagatt
     1921 ctgcttcacg aaccacttca tcttcaaaag gcgtccacac acccgactcc aggacaagta
     1981 gcgtcacatc cgagcgg
//
LOCUS       DAOSIO010000236         1992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_49091_length_1992_cov_3.887971, whole genome shotgun sequence.
ACCESSION   DAOSIO010000236 DAOSIO010000000
VERSION     DAOSIO010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49091_length_1992_cov_3.887971"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1769)
                     /locus_tag="PK590_07555"
     CDS             complement(<1..1769)
                     /locus_tag="PK590_07555"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOG24490.1"
                     /translation="MDEKRYNPFMRKILPYLVPALVLAGILFLLCHFSVPIEDFRIMV
                     FDNYQRIHPRVYKDTPVRIIDIDDKSLERFGQWPWPRTVLARLVDRLHKAGVQTLAFD
                     VVFAEPDRTSPQSVISSWPDVPALRELRAQLSALPDHDALFAEVSSEARVVFAFGLIN
                     ETTKNFPTIKFGGFLEHGTLGDQAIDYIRPAYRGAVTNLPALESAAKGLGGFNVSSDE
                     KDMVRRASALFRLRETIYPSLALEAMRVFQEASAYKISLAGTARHRSFGEKTGIVSIK
                     TGFLDIPTDKHGRIWLFDTGHKPERFIPAWKIFSADFDLSSLDGKLVFVGTSATGLKD
                     LRPTPLNAFAPGVEVHAQIAEQMFTGDYLHRPDWTEGAEFLYILVLGLLLILLLPSVG
                     AIWCALLGASAIGFAIWFSWNQYVEKQLLIDPLVPSLSILLIYLVSSFIRFVKTETER
                     GQIRSAFSRYLSPALVGQLAKNPKQLKLGGETREMSFLFMDIRGFTTLAEGMSAEELT
                     NFMNSFMTPMTEIILKHNGTIDKYIGDCIMAFWNAPLDDVDHARHACQAALEMRDFIQ
                     TFNAARKEKTESRGKPFKEVKIG"
BASE COUNT          479 a          494 c          564 g          455 t
ORIGIN      
        1 atcccgattt tgacttcctt aaaaggtttc ccacggcttt cggttttttc ttttcgcgcg
       61 gcgttgaatg tctgaataaa atcccgcatt tcgagcgccg cctggcacgc gtgcctcgcg
      121 tgatcgacat cgtcaagagg cgcgttccag aatgccatga tgcaatcacc gatgtattta
      181 tcgatcgttc cgttatgttt taaaataatc tcggtcatgg gagtcatgaa ggaattcatg
      241 aagttcgtaa gctcttcggc gctcatgccc tccgcgagag ttgtgaagcc gcggatgtcc
      301 ataaaaagga aagacatttc tcttgtttct ccgccgagct ttagctgttt cggatttttg
      361 gcaagctggc cgaccaaagc gggggagaga taccggctga acgcgctccg gatttgcccg
      421 cgctcggttt cggttttcac gaaccggatg aacgaagaaa cgagataaat cagcaaaatg
      481 gacagggacg gcacgagagg gtcgatcaac agttgttttt caacgtactg gttccatgaa
      541 aaccagatcg cgaagccgat agccgacgct cccagtaacg cgcaccagat cgcgccgacg
      601 ctgggcagga gcaggatcag gaggaggccc aggacaagaa tgtacaggaa ttccgctccc
      661 tccgtccaat cggggcgatg gagataatcg ccggtgaaca tctgctccgc gatctgggcg
      721 tggacttcca cgccgggcgc gaacgcgtta agaggcgtcg gccgaaggtc tttcagaccg
      781 gtcgcgctgg ttcccacaaa gacaagtttc ccgtcgagag acgacaagtc aaaatcggcg
      841 gagaaaattt tccaggcggg gatgaatcgc tcgggtttgt ggccggtatc gaaaagccaa
      901 attcttccgt gtttgtcggt cgggatatcc aggaaaccgg ttttgatcga gacgatccct
      961 gttttttccc cgaaagagcg atggcgggcg gttcccgcga gcgagatttt gtaggcgctg
     1021 gcttcctgga acactctcat ggcttcgaga gccagagaag gataaatggt ttccctcaat
     1081 ctgaaaagcg ccgacgcccg tcgaaccatg tctttttcgt cagagctgac attgaaacct
     1141 cccagccctt tggcggcact ttcgagagcc ggaagattgg tgaccgctcc ccgataggcc
     1201 ggacggatgt aatcgatggc ctggtcgcct aatgtgccgt gttcgagaaa tccgccgaat
     1261 ttgatggtcg ggaaattttt agtggtttcg ttgatcaagc cgaaggcgaa aacaacgcgc
     1321 gcctccgaac tgacttcggc gaaaagagcg tcatgatcgg gaagcgctga aagttgcgcg
     1381 cgaagttccc ggagcgccgg gacatcaggc cacgaggaga tcacgctttg gggagatgtc
     1441 cggtcaggct cggcgaaaac aacgtcaaag gcaagggttt ggacgccggc cttatgaagg
     1501 cggtccacga gtctcgcgag aacggtacgg ggccacggcc attggccgaa gcgttcgagg
     1561 cttttatcgt cgatatcgat aatccgcacg ggggtatctt tgtaaacgcg gggatggata
     1621 cgctggtaat tatcaaaaac catgatccgg aaatcttcga tcggaacgga aaaatggcaa
     1681 agaaggaaaa gaatgccggc aaggaccaga gcggggacaa gatagggaag tatctttcgc
     1741 atgaacggat tatatcgttt ttcatccatg gcgcaactcg ggatgttgtt tcaggtgaaa
     1801 acgtgtcact tctctatcct cccattttct caaagtagtt tcatggggct attctttatt
     1861 ttccagccat tagaacaacc accggcttaa acgcttttgg ggccaattcc atccgcggga
     1921 ggagggatac accccacccc gttgcctcat attaaaacct taccatgaga attgtggatg
     1981 actcgtaatg tg
//
LOCUS       DAOSIO010000237         1959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_50256_length_1959_cov_3.327731, whole genome shotgun sequence.
ACCESSION   DAOSIO010000237 DAOSIO010000000
VERSION     DAOSIO010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50256_length_1959_cov_3.327731"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..706)
                     /locus_tag="PK590_07560"
     CDS             complement(110..706)
                     /locus_tag="PK590_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24491.1"
                     /translation="MKAIVLEQVDDLFSDKKKLSKIERGLRDYQWIQTRVNEVDVSAD
                     HLFQERYSVFYVMWFRPKEWKKCYFELFEQAKKMEPRPDFREILKCLNERTGKMEASF
                     ASKLVATLNPAKQIIDKYVLENFELILPPLNRVNRLEETVGVYEKLGALYNDFLKEPI
                     CGEICRRFNKSFSVSNVTQLKMIDFVLWQIRPKNTSSM"
     gene            complement(717..1661)
                     /locus_tag="PK590_07565"
     CDS             complement(717..1661)
                     /locus_tag="PK590_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HOG24492.1"
                     /translation="MKNKNYDKKIFRLVSILNKLNGGGKVRSSELAGEFNVSIRSVQR
                     DLDLLNLTGFPLDTQEKGIYSFMPGFSLRKLMLTNEEASLLSFFHEIADSLGKNFSES
                     FRSLVSKVLAREDSSPFFVKLPEIPAHMKNFPFVKELEQAVNESQKIQIKYESANGIK
                     EHRLCPLKLVNFEGFWYLLAMPDGKRQFRKFRVEKIKGLELLDENFNPPRNLKAALER
                     SVNIWFSEEPGEKILLEIDKDAAHYFKKKIYFPEQRIIKQKKDGTLILQTQANHNEVL
                     RTIIQWIPDIRVIKPKELDLKVRQLLSAYLKGSRPRWG"
     gene            complement(1685..>1959)
                     /locus_tag="PK590_07570"
     CDS             complement(1685..>1959)
                     /locus_tag="PK590_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24493.1"
                     /translation="PKVGKQIMPGNEQWNKFFDMKLSDITKERDVLYLRDVLKKADKK
                     PGAPGIHFRYSIDRGSPVLTKPEELTQEVISTMVQFYPILAYLEGK"
BASE COUNT          487 a          490 c          430 g          552 t
ORIGIN      
        1 gttgtaaaat ctacaaaaag gagtgtagcc gaagacagcg acagcattat gtcgcttgta
       61 tttagtgcgt ggttttttgc aaaatactag gggtagagct gcgagggagc tacattgagg
      121 aggtgttttt aggccttatt tgccaaagga caaaatcgat cattttaagc tgggtgacgt
      181 ttgagaccga aaagctctta ttaaaccgcc gacagatctc gccacagatc ggctctttta
      241 aaaaatcatt atataaagcg cccagttttt cataaacacc aaccgtttcc tctaaacgat
      301 tgaccctatt gaggggaggc aagatcagct caaaattttc gagaacgtac ttatcgatga
      361 tttgtttcgc aggattcaat gtcgcgacca acttgctagc gaacgatgcc tccatcttcc
      421 cggtacgctc gttcagacac ttaaggattt ctctgaaatc aggcctcggc tccattttct
      481 tagcctgttc aaatagctca aagtaacatt ttttccattc cttcgggcga aaccacatca
      541 catagaatac gctgtaacgc tcctgaaaaa gatggtccgc gctaacatcg acttcattaa
      601 cacgggtttg aatccattga taatctcgca gcccccgctc gattttcgag agctttttct
      661 tgtcagaaaa caggtcgtca acttgttcca gcacaattgc tttcatgctt cgcaacttat
      721 ccccacctag ggcgggagcc tttgagatag gcgctcagga gttgccggac cttcagatcg
      781 agctctttcg gcttaatgac ccggatgtcc ggaatccatt ggataatggt gcggagcact
      841 tcgttgtggt tagcctgagt ctggaggata agagtaccgt cttttttctg tttgatgatc
      901 ctttgctctg ggaagtagat cttcttcttg aaatagtggg cggcatcctt gtcaatttca
      961 agcaggatct tctcccccgg ctcttcgctg aaccagatgt ttacgctccg ctccagggcc
     1021 gcttttaggt tccgcggcgg attgaaattc tcgtccagaa gctcaagtcc tttgatcttt
     1081 tcaacccgga acttacggaa ttgacgcttc ccgtccggca tggcaagcag gtaccagaac
     1141 ccttcaaagt tgaccagttt aagcggacac aaccggtgct ctttaatgcc gttcgcggat
     1201 tcatatttga tttggatctt ctgtgactcg ttgaccgcct gctcaagctc cttaacaaat
     1261 gggaagttct tcatatgcgc cgggatctcg ggcaatttga caaagaaggg cgaggaatct
     1321 tcacgcgcga ggactttact cacaaggctc cgaaaagact ccgagaagtt cttcccaagc
     1381 gaatctgcaa tctcgtgaaa aaaagatagg agtgacgctt cctcattggt cagcatcaac
     1441 ttcctgagcg agaaccccgg catgaagcta tagatccctt tttcctgcgt atcgagcggg
     1501 aagccggtca ggttcaggag atcgaggtcc cgctggacgc tccggatcga gacattgaac
     1561 tcccccgcaa gctcgctcga gcggaccttc ccgcccccgt tcagcttgtt caggatcgat
     1621 accagccgaa agatcttttt gtcgtagttt ttatttttca cagacttcct catagcagac
     1681 aattctattt accttccaaa taagccaata tcgggtaaaa ctgtaccatt gtgcttataa
     1741 cctcttgcgt tagctcttca ggctttgtaa gaactggtga tccgcgatca atagaatatc
     1801 gaaaatggat acctggagct cccggcttct tatctgcttt ctttaataca tcccgaagat
     1861 aaagaacatc tcgctcttta gtaatatcac ttaatttcat gtcgaaaaac ttattccact
     1921 gctcattccc cggcatgatt tgcttcccaa ctttcggca
//
LOCUS       DAOSIO010000238         1928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_51376_length_1928_cov_4.113721, whole genome shotgun sequence.
ACCESSION   DAOSIO010000238 DAOSIO010000000
VERSION     DAOSIO010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51376_length_1928_cov_4.113721"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /gene="glyS"
                     /locus_tag="PK590_07575"
     CDS             <1..1250
                     /gene="glyS"
                     /locus_tag="PK590_07575"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOG24494.1"
                     /translation="GEYTLTVDELGFVAVMNGKRSGLSKIKADYESVLESRLKDARYF
                     YEADTREKLESKKGLLSQLVYLGKLGSMLEKTERLEKLAPVFADMTGKKDLADDLART
                     ACLSKIDLMTNLVYEMPDLQGIVGREYALESGENQSVALAIGTQYLPKNLSEDHKAVA
                     RSMTLLGALFGVMDRLDLLVGAFGTGIEPTGSQDPFALRRAGGAIVKLIRVFELRFSL
                     TQMIKANIELYGDRLNKKEKLTERILKFLQDRTVFESGVKPGTREFEILQAVMKARFD
                     DVADVFRRYGVLSGMDEKVLVRAAKVIQRTANMLKGYGKTPGEIRPELFTEEQEKRLY
                     EIIRLKAGEVSGLIESGAYGKGTELFARTFSHPLHDFFDHVLVNTEELSVRDNRMALV
                     GLVNRIYTEKLADLSLLSRLDEE"
     gene            1404..>1928
                     /locus_tag="PK590_07580"
     CDS             1404..>1928
                     /locus_tag="PK590_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020150235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOG24495.1"
                     /translation="MSTATRRRSGKSGSKKSKSSKSKYVYFFGAGKAEGDAKMKQLLG
                     GKGANLAEMTNLGVPVPAGFTITTETCDLFYRNGKKWPVGLEQQVRDNIVKLEKAMGL
                     KFGDRNNPLLVSVRSGAAVSMPGMMDTVLNLGLNADVVQGLIAKTENERFAWDSYRRF
                     IQMFGDVVMEVPHDE"
BASE COUNT          468 a          437 c          572 g          451 t
ORIGIN      
        1 ccggcgagta cacgctgacg gtcgacgagc tcggcttcgt cgcggtgatg aacggaaagc
       61 ggtcgggact ttcgaagatt aaagcggatt acgagagcgt tctggaatcc cggttgaaag
      121 atgcccgcta tttttatgaa gctgataccc gggaaaaact ggaatctaaa aaaggacttt
      181 tgagccagct ggtgtatctt ggcaagctcg gctcgatgct tgaaaaaacg gagcggcttg
      241 agaaactcgc gcctgttttc gcggacatga cggggaagaa ggatctcgcg gacgatctcg
      301 cgcgcacggc ctgtctgtcc aaaattgacc tcatgacgaa tctggtttat gaaatgccgg
      361 atctccaggg gattgtgggg cgtgagtacg cgctcgagtc cggcgagaat caatcggtgg
      421 ccctcgcgat agggacccaa tatctcccta aaaatctctc ggaagatcac aaggcggtgg
      481 cgcggtccat gactttactg ggcgcccttt tcggggttat ggaccgactg gatcttttgg
      541 tgggcgcctt tggaaccgga attgaaccga ccggaagtca ggatccattc gccttgaggc
      601 gcgccggcgg cgcgatcgtg aagttgatcc gcgtgtttga gttgcgtttt tctctgacgc
      661 aaatgatcaa ggccaatatc gaactttacg gggatcgttt gaataaaaaa gaaaagctga
      721 ccgaaaggat tctcaaattt cttcaggatc gaacggtgtt tgaatccgga gtgaaacccg
      781 gtacccggga gtttgaaatt ttgcaggcgg tcatgaaggc tcgttttgat gatgtcgcgg
      841 atgtgttcag aaggtacggg gtgttgagcg gcatggatga aaaagttctt gtccgggccg
      901 cgaaagtgat tcagcggacg gccaacatgt taaagggata cgggaaaact ccgggagaaa
      961 tcagaccgga gctttttacg gaggagcagg aaaaaaggct gtatgaaatc attcgtctga
     1021 aggctgggga agtttccggg ctgatcgaaa gcggtgcgta cgggaagggg actgagttgt
     1081 tcgcgcggac attctctcat ccacttcacg acttttttga tcacgtgctt gtgaatacgg
     1141 aggagctgtc ggtccgggat aaccggatgg cgcttgtggg tttggtgaac cgtatttata
     1201 cggaaaagct ggccgatctt tctttgctat ccagacttga cgaagaatga tataatccac
     1261 gctcttcttt gccctgcctg aaaccccgcg cgggtcagtc aaaggggtaa attaaaattg
     1321 cattctataa ccaacaagtc catcgtttcg ccttttagag gcgggggcgc tggacgacta
     1381 ttataaggag tgaaagaaac accatgagta ccgctacgcg caggagatcc ggcaagtcag
     1441 gttccaaaaa atccaaatct tccaagtcta aatacgtcta tttttttggc gccggcaagg
     1501 ccgagggtga tgccaagatg aaacagctcc tgggcggcaa gggagcaaat ctcgcggaaa
     1561 tgaccaacct tggcgtcccg gtccccgccg gttttacgat taccaccgaa acctgcgatc
     1621 tgttctaccg gaacggaaag aaatggccgg tgggtcttga acagcaggtt cgtgacaata
     1681 ttgtcaaact cgaaaaagcg atggggctta agtttggtga ccggaacaat ccgcttctcg
     1741 tttctgtccg ttccggcgcg gcggtttcga tgccgggcat gatggacacg gttctgaacc
     1801 tgggtctgaa cgccgacgtc gtgcagggac tcattgcaaa gaccgaaaac gaacgctttg
     1861 cctgggattc ctatcgtcgt tttatccaga tgttcggcga tgtcgtgatg gaagtgccgc
     1921 acgatgag
//
LOCUS       DAOSIO010000239         1880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_53236_length_1880_cov_4.210959, whole genome shotgun sequence.
ACCESSION   DAOSIO010000239 DAOSIO010000000
VERSION     DAOSIO010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53236_length_1880_cov_4.210959"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PK590_07585"
     CDS             <1..201
                     /locus_tag="PK590_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24496.1"
                     /translation="FLVELKFHAIMHGATFAEVPIVFTDRINGDSKISKKVIRESVFF
                     VLKLSRHRAKVRWVISGMRDRK"
     gene            188..1756
                     /locus_tag="PK590_07590"
     CDS             188..1756
                     /locus_tag="PK590_07590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOG24497.1"
                     /translation="MTESKRSGPGRLYVVFWGITLFLVLGLQFFLIQRPFRGHFASYQ
                     GVMAEIGRNMIREDFSEPLLPKTDLLIVLPTRSLHLNQYPLPSLLAALGVRWIGGGFE
                     FWGRFQAIVFNLLSLLLMGGIATQWWGKRTGFFAAWLYLTAPFSIVYGQMFMSESIAL
                     FFLLSSFYLIERMAKDGAGPLKIVLAGLCFSVSALNRVHFVFLFPAFLYWAFKEGRKH
                     KIGYAFLFCALSGLLPLLWYGFTYCFGKGPEMLRVHTSLFFQMGAPGLTRFGDVFSME
                     YGKYLARIILFFMMTPVVWPFFVVGIRRIAGERRFSVLYSLLAMTCLFLAFAMPQKIL
                     EQGFYFYGFFPFFVFVVAKGLDDVMDQQWFRKFRGPILLLYALCVLRFSIPPLFFYPP
                     EEKNILVPTAQARAVSNPDDIFIVGGQGASAASYYLDRKMYGLQPQTSRSLATYYRKR
                     PYDERSKDAIRSYETAAQDDITYVEYLRGKGASYVLILDKPSSDFNDRLIPYLCSRYR
                     ELSAEVDSFYLFKI"
BASE COUNT          370 a          421 c          494 g          595 t
ORIGIN      
        1 tttttagtgg agttgaagtt ccacgcgatc atgcacgggg cgaccttcgc ggaggttcct
       61 atcgtgttca ctgatcggat caacggagat tccaagatct ccaaaaaggt catacgtgaa
      121 tcggtgttct ttgtgctgaa attatcacgg catcgtgcga aggtcaggtg ggtgatcagt
      181 ggaatgcgtg accgaaagta agaggtctgg tcccggtcgg ctctatgttg ttttttgggg
      241 gatcacgctt tttctggttt tgggacttca gttttttttg atccagaggc cgtttcgggg
      301 acatttcgcc agttatcagg gggtgatggc tgagatcggc cggaacatga tccgcgagga
      361 tttttccgaa cccttgctac ccaaaacgga tttgctgatc gttcttccca cgcgcagtct
      421 tcaccttaac caatatccat tgccgtccct attggccgct ttgggagttc gatggatcgg
      481 tggtgggttt gaattctggg ggcgctttca ggcgatcgtt tttaatctct tgagtttgct
      541 cctgatggga ggcatcgcga cccaatggtg gggaaagcgc actggttttt tcgccgcgtg
      601 gctttatctt acagcaccct tttccattgt ctacggccag atgtttatgt cggaatcgat
      661 agcgctgttc tttcttctct ccagctttta cctgattgag cgaatggcga aggatggagc
      721 cggccccttg aagatcgttc tcgccggcct ttgtttttcc gtgtccgctc tcaaccgtgt
      781 tcacttcgtt tttctttttc cggcgttctt atactgggcg tttaaggaag gacgaaagca
      841 caagatcggg tacgcgttcc ttttttgcgc cctctcggga ctgctgccgc tcttatggta
      901 tggatttacg tattgcttcg gcaaggggcc ggaaatgttg cgcgttcaca caagcctctt
      961 cttccaaatg ggggcgccag ggctgacacg attcggcgat gtgttctcga tggagtatgg
     1021 gaagtatctt gcgcggatca ttttattttt tatgatgact ccggttgttt ggccgttctt
     1081 tgtcgtgggg atccgccgga tcgctgggga gagaaggttt tctgttttgt attcgcttct
     1141 ggccatgacg tgtctgttcc tcgcgtttgc gatgccacaa aagatcttgg aacaagggtt
     1201 ctatttctac gggtttttcc cgttttttgt ttttgtggtg gcaaagggcc ttgacgacgt
     1261 aatggatcag cagtggttcc ggaaatttcg aggaccgatc ctgcttttgt atgcgttgtg
     1321 cgtactgcgc ttttcaattc ctcctctctt tttctatcct ccggaggaaa aaaacatctt
     1381 agtgccgacc gcccaggcac gcgcggtgtc taacccagac gacattttca tcgtgggcgg
     1441 ccaaggggcg tctgccgcaa gttattatct ggatcggaag atgtacggtt tgcaacctca
     1501 aacctcgcgt agtttggcga cttactatcg caaacggccg tatgatgaac gatcgaaaga
     1561 tgcgatccgt tcgtatgaaa cagctgcgca agacgatatt acgtatgtag agtatcttcg
     1621 ggggaagggg gcttcctatg tgctgatcct cgataagcca tcatctgatt ttaatgacag
     1681 attgatcccg tatctttgtt ccagataccg tgaattatct gcggaggtcg attcctttta
     1741 cctctttaag atttgattat tttcattcgt acgttgtttc ctgtaagggg cgagaccttt
     1801 attgcaatag atataaagcc acatctattg acatctaatg acttcaaagt tatattttgc
     1861 tatattttaa attaatcaaa
//
LOCUS       DAOSIO010000240         1847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_54568_length_1847_cov_3.061942, whole genome shotgun sequence.
ACCESSION   DAOSIO010000240 DAOSIO010000000
VERSION     DAOSIO010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54568_length_1847_cov_3.061942"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PK590_07595"
     CDS             complement(<1..388)
                     /locus_tag="PK590_07595"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24498.1"
                     /translation="MTKKKSALSAFILRPSFLSLLTGVLLTLSYPFPGWKFLAWVALI
                     PLFYALRLTSSGAQALGLGYLAGFVFFTLSMHWLVHVTLVGWLLLAAFEALFFAVFAW
                     LVFWGKRTEWPTICKILWTASSWTLTE"
     gene            complement(415..486)
                     /locus_tag="PK590_07600"
     CDS             complement(415..486)
                     /locus_tag="PK590_07600"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOG24499.1"
                     /translation="MGRNDPCPCGSGKKYKKCCGVNE"
     assembly_gap    522..531
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<532..>1847)
                     /gene="secA"
                     /locus_tag="PK590_07605"
     CDS             complement(<532..>1847)
                     /gene="secA"
                     /locus_tag="PK590_07605"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; proteins in this cluster correspond SecA1;
                     SecA2 is not essential and seems to play a role in
                     secretion of a subset of proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOG24500.1"
                     /translation="PTHRPCVRQDHHDSVYRTEKEKFDAVVREIAEVHKAGRPALVGT
                     ISIEKSEKLSKLLHRINLPHTVLNAKYHEKEAEIVAQAGQPGAVTIATNMAGRGTDIV
                     LGPGVKEKGGLAVIGTERHESRRIDNQLRGRCGRQGDPGTSKFFISLEDELMRLFGSD
                     RISGLMQRLGMQEGDEIQHPFVNRAMETAQKRVESYNFEIRKHLLEYDDVMNRQRELI
                     YGERDLVLKGSYEELKKHIFEMAENVVEDLLLAYVNPDLRDDEQDPEGLALALGEKLG
                     ADFRKAIEENMYGIESLQAELNEQIVALYNAREQAFGIERIHFLEKYILLQVIDSKWK
                     EHLYSLDSLREGIGLRAYGQRDPLVEYKREAFALFDSMTEAIKGEAIGLLFRVQVVRE
                     EKMRSEPGAPRAQYLHPESGGMKKTAERAYQNVERGLDTQSHFAPR"
BASE COUNT          435 a          535 c          435 g          432 t
ORIGIN      
        1 actccgtcag cgtccacgag gaggccgtcc agaggatttt gcaaatcgtg ggccattccg
       61 ttctttttcc ccaaaacacc agccacgcga aaaccgcgaa aaataacgct tcaaacgcgg
      121 caagcaaaag ccagcccacc agcgtcacat gaacgagcca gtgcatggac agggtaaaaa
      181 aaacaaatcc cgcgagatag ccaagtccca aagcttgggc gccagaagaa gtcaaacgaa
      241 gggcgtaaaa aagaggtatg agggcgaccc acgcgagaaa tttccatccc gggaacggat
      301 aagacaaagt cagtaaaaca cctgtcaaca aagaaaggaa ggatggacgg agaatgaagg
      361 ctgaaagagc agattttttt ttcgtcattc gtcccccctc attcgctttt caccttactc
      421 attgacacca cagcacttct tgtacttttt accgctacca cacggacagg gatcgtttcg
      481 tcccactttg ggagactcgc gatggatggg ctcgggcttt tnnnnnnnnn ngtcggggag
      541 cgaaatgtga ttgcgtgtcc agcccgcgtt ccacattctg ataagccctt tccgcggttt
      601 ttttcattcc cccggattcc ggatgcaagt actgcgcgcg cggcgccccg ggttctgaac
      661 gcattttctc ttcacgcacc acctgaaccc gaaaaagaag tcctatagct tcccccttga
      721 tggcttccgt catggaatca aaaagggcaa acgcttccct tttatactcc acaagaggat
      781 cccgctgtcc atacgcccgc agaccaatcc cctcgcgcag actatcaagc gaatagagat
      841 gctctttcca tttcgagtca atgacctgta gcagaatgta cttttcgaga aaatgaatgc
      901 gttcaattcc gaacgcttgc tcgcgcgcgt tgtagagcgc gacaatctgt tcgttcaact
      961 ccgcctgtag ggactcaatt ccgtacatat tttcctcgat ggctttccgg aaatcggctc
     1021 ccagcttctc cccaagcgcc agagccaacc cctcgggatc ttgttcatca tcacgaagat
     1081 cgggattgac gtaggctaaa agcaggtctt caaccacgtt ctcggccatt tcaaaaatat
     1141 gttttttgag ttcctcgtag ctccccttga gcacaaggtc ccgttcccca taaatcagct
     1201 cgcgttggcg gttcatcaca tcgtcatatt caagcaggtg tttgcggatt tcgaaattgt
     1261 aagattccac tctcttctgc gccgtctcca tcgcgcggtt cacaaaggga tgctggattt
     1321 cgtcgccctc ctgcatcccg agacgctgca taagacccga tatccggtcg gaaccaaaaa
     1381 gacgcatcag ttcatcttca agcgagataa aaaacttgga cgtccccgga tctccctggc
     1441 gcccgcatct cccccgaagc tgattgtcaa tgcgccggga ctcgtggcgt tccgttccaa
     1501 tcacagccag accgcccttt tccttcactc cggggccaag cacgatgtcg gttccacgcc
     1561 ccgccatgtt agtcgcgatc gtgaccgcgc cgggctgtcc ggcctgggcg acaatctcgg
     1621 cctctttttc gtgatatttg gcgtttaaaa ccgtgtgagg aagattgatc cgatgaagca
     1681 acttgctgag tttttccgat ttttcgatcg agatcgtacc gaccagcgcg ggtcgccccg
     1741 ccttgtgaac ttccgcgatc tcgcgaacaa cggcgtcaaa cttttctttt tcagtccggt
     1801 agacagaatc gtgatgatct tgcctgacgc acggacgatg ggtcggg
//
LOCUS       DAOSIO010000241         1831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_55201_length_1831_cov_4.251689, whole genome shotgun sequence.
ACCESSION   DAOSIO010000241 DAOSIO010000000
VERSION     DAOSIO010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55201_length_1831_cov_4.251689"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1831
                     /locus_tag="PK590_07610"
     CDS             <1..>1831
                     /locus_tag="PK590_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24501.1"
                     /translation="VTPETSTGQPATAKPNTIAEALNAPDFEPKKLIEALKKVLSLGP
                     LYSMSVGVWEGYTLEEHTLLAMSQFEKYFSATQLPGKGDKQIFRILLALHDIGKPQAV
                     ADGRKEEQHARTLEIINEIKEELPLSPEQLNVVMSLVDGDPIGLFIRGRADLESTVEE
                     IRIKAEKAGLTAAEFFDLLTIYYQMDASSYTADSGGKASLEFLFVKDQERNRFAFDPV
                     KKRLKFSPKVQALFNALEVAVRAEYLDRLIKSSNMLSSFKKYSTVAELKEALPGRFMT
                     IQDFVKTKARWVRGGYSLGLSKGYHLYFGWSRLCGLIDWMTAYALELIGFVEPGHTDA
                     FLSQITRDNKLIVFFVPDGMWDSGVTRDEMNWIAEKLQSKPDAFNILFVFGAYDTYFS
                     SADLQVYTQEGKEAFDDLMATRVLEDLGSGMSDEEREKLEEDINSNIKEYEERRKKAY
                     RKRKLGDALRNLADPSRIAALRLLEAVKSANSKKPKASKAGEPSADSRPEASDSRSES
                     WESWEGATTDPRQYKKGGAFRFIVHAAQGEESYIWRSVFAAQQRLEVDPSRSINLRRE
                     PQRIAEKTMISASYIDQNHRATWGPAGFILKVPEGNILETHTQD"
BASE COUNT          507 a          361 c          503 g          460 t
ORIGIN      
        1 gtgacgcctg aaacatccac cggacaaccc gcgaccgcga agccaaatac tatcgctgaa
       61 gcgctgaacg cgcctgattt tgagcctaaa aaactgatag aagcgcttaa aaaagtattg
      121 tcgctggggc cgctgtattc aatgtcagtg ggtgtatggg agggttatac attggaagaa
      181 catactcttt tggcaatgtc ccaattcgaa aaatattttt cggcaactca gttaccggga
      241 aaaggtgaca aacaaatttt caggattttg ttggcattgc atgatattgg taagcctcaa
      301 gccgtcgcgg atggtcggaa ggaagaacag cacgcgcgca cattggaaat cattaatgaa
      361 atcaaggagg aactgccact tagtccggag cagttgaatg tcgtgatgag tttggtggat
      421 ggtgatccga tagggctctt tattcgagga cgcgctgatc ttgagtcgac ggttgaagaa
      481 attcgcatta aggcggaaaa ggcaggatta acggcggccg aatttttcga tttattgacc
      541 atctattacc aaatggatgc cagcagttat acagcggatt caggaggaaa agcctcgctg
      601 gaatttttgt tcgtgaagga tcaggagagg aaccgttttg cgtttgatcc cgttaagaag
      661 cggctcaagt ttagcccaaa ggttcaagcg ctatttaatg ctttggaagt agcggttagg
      721 gcggaatatc ttgaccggtt gattaaaagt agcaacatgt tgagttcttt taagaaatat
      781 tccacagtcg ctgaactaaa agaagcgttg cccggacggt tcatgacgat tcaagatttt
      841 gttaaaacta aagcgcgatg ggttcggggc ggctattcgc tgggtttatc gaaggggtat
      901 cacctttatt tcggatggtc gagactttgt gggcttattg attggatgac cgcttatgcc
      961 ctggagctga ttgggtttgt tgagccgggc cacacagacg cttttctgag tcaaattacg
     1021 agggacaata agcttatcgt gttttttgtg cctgacggca tgtgggactc cggcgtaaca
     1081 agggatgaaa tgaactggat agctgaaaaa cttcaaagca agcctgacgc gttcaacatc
     1141 ctatttgtat tcggcgctta tgacacatat ttttcatctg ccgatcttca ggtctatacc
     1201 caggagggca aggaggcttt cgatgatttg atggccacac gggtgcttga agatctcggt
     1261 tcagggatgt cagatgagga aagagaaaag ctggaggagg atattaattc taacattaag
     1321 gagtatgaag aaagacgcaa gaaagcctat agaaagagaa aactgggaga cgcactgcgt
     1381 aatctagccg atccatcgcg cattgcagcc cttagattgc tcgaggcggt caaatcggca
     1441 aatagcaaga aacctaaggc gagtaaagca ggcgagcctt cggcggatag ccgtcctgaa
     1501 gcttctgatt cccgttccga atcgtgggaa agttgggaag gtgccaccac agatccgcga
     1561 cagtataaaa aagggggcgc gttcaggttc attgtgcatg cagcgcaagg tgaagaaagt
     1621 tatatttggc gctccgtgtt tgcggcacag caaaggcttg aggtcgatcc gtctcgtagc
     1681 atcaatttgc ggcgggaacc ccaacgaatt gctgaaaaaa ctatgatttc tgcttcttac
     1741 atcgatcaaa atcatcgtgc gacatggggg cctgcgggtt ttattttgaa agttccggag
     1801 ggaaatattc tcgaaacaca cacgcaagat g
//
LOCUS       DAOSIO010000242         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_55240_length_1830_cov_3.441127, whole genome shotgun sequence.
ACCESSION   DAOSIO010000242 DAOSIO010000000
VERSION     DAOSIO010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55240_length_1830_cov_3.441127"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PK590_07615"
     CDS             complement(<1..258)
                     /locus_tag="PK590_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24502.1"
                     /translation="MAVFIVYLIIAVAIVYFIQRRDRPSSPGGTDMSPARKKTAEARK
                     EAREVWKQVYETASMDEARGIQERLESENIRCILYEQGKKDI"
     gene            623..1201
                     /locus_tag="PK590_07620"
     CDS             623..1201
                     /locus_tag="PK590_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009021251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HOG24503.1"
                     /translation="MPFELSKLPYEIESLTPFLSKETLEYHYGKHHQTYVTNLNKLTD
                     GKPEASQSLENLIRTAKGGIFNNAAQVWNHTFYWNCLKPQGGGEPDGAIAEAINASFG
                     SFEEFKTFFSNAAIALFGSGWVWLVRDEDGTLGVESLSNAGNPLTQNQKPLLTIDVWE
                     HAYYIDYRNARAKYVEAFWSAVNWEFVNQQLG"
     gene            complement(1249..>1830)
                     /locus_tag="PK590_07625"
     CDS             complement(1249..>1830)
                     /locus_tag="PK590_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG24504.1"
                     /translation="PNRVPRLVTRNATILSCNYSNSDKNFRTGSMDPFDINKEENAQR
                     FLAEAYEKQMAGQIDEAIALYLKSIEASPSAEAHQYLGWAYSLKGRFGDAIRECEKAI
                     QIDPQYGNPYNDIGAYLIETGRWSEAISWLEKATAAERYEGRFYAWYNLGRVFEYEGD
                     WMKALGAYRHALEQNPQYTLAEKAIRRMESALN"
BASE COUNT          450 a          470 c          420 g          490 t
ORIGIN      
        1 aatatctttt ttaccctgct cgtagagaat acaccggata ttctccgact ccagacgttc
       61 ctgaatcccc cgagcttcgt ccatggaagc cgtttcataa acctgcttcc agacctcacg
      121 cgcctctttc ctggcttcag ccgttttttt tcttgccggg gacatatcag tcccgcccgg
      181 cgaagaaggg cgatcacgtc tttggataaa atagacgatc gccaccgcaa tgatgagata
      241 aacgataaac actgccacag agaactcctt ttaaatcatg gatctatccg gatccgctgg
      301 agggatttcc ttccggcttc cagtttgaag tttacctgaa gcaaaaaacc gctcgcctac
      361 atccaaatca gaagtgtatt ttgtcccgtc ctcacacgaa ggtcaagcga tatcaaattt
      421 ttgatcgaat tttgaggaaa aattgggcgc ttgggttgtg tttgcgttgt gttataatga
      481 cgtctcatga gtggcggatt tgcttggcca ctacctttta agaccaaatc cggttttaaa
      541 tttaaaaaat gtgaagtccg gtgagcgacc tttcgttttc agaatgcctg ttgatcactc
      601 ataacctcaa ggggtaaaaa ttatgccgtt cgaactgtca aaattgcctt atgaaattga
      661 atctctcacg ccttttctct caaaagaaac tctggagtat cactacggaa aacatcacca
      721 gacatacgtc acgaatttaa acaagttaac cgacgggaaa cccgaggctt cgcaatcgct
      781 cgaaaacctg atccgcacgg caaagggcgg cattttcaac aacgcggccc aggtgtggaa
      841 ccacacattc tactggaatt gtctgaaacc acagggcggc ggtgaaccgg acggagcgat
      901 cgctgaagcc attaacgctt ctttcggaag tttcgaggaa tttaaaactt ttttttccaa
      961 cgcggcaata gccctgtttg gctcggggtg ggtatggctc gtcagagacg aggacggaac
     1021 gctaggcgtt gaaagccttt ccaatgccgg aaatcctctc acccaaaatc agaaaccact
     1081 cctcacgatt gatgtctggg aacacgctta ttacatcgat taccggaatg cccgcgcgaa
     1141 atacgttgaa gcgttctgga gcgccgtcaa ctgggaattt gtcaatcagc agttagggta
     1201 gatcataaaa gacgggcgcg gaccaacggg atccagtttt ttccctgatc aattcagcgc
     1261 ggattccatt ctgcgaattg ctttttcggc cagtgtgtat tgtgggtttt gctccagcgc
     1321 gtggcgatac gcgcccagcg ccttcatcca gtctccctca tattcaaaca ctcgaccaag
     1381 attgtaccag gcgtaaaagc gtccttcgta acgctccgcc gctgttgcct tttcaagcca
     1441 ggaaatcgct tccgaccatc gtcccgtttc gatcagatag gctccaatgt cattgtaggg
     1501 gtttccgtac tgtggatcga tctggatagc tttttcacat tcccggatcg cgtcaccgaa
     1561 ccggcctttc agactataag cccagccgag atactgatgg gcctcggcgc tcggactagc
     1621 ttcgatggat ttcagataaa gggcgatcgc ctcatctatc tgtcccgcca tttgtttctc
     1681 gtatgcctcg gccagaaacc gttgggcgtt ttcctcttta ttgatgtcaa aaggatccat
     1741 agagcctgtc ctgaaatttt tgtcactgtt tgaataattg catgaaagga ttgtcgcgtt
     1801 tctcgtcacc aatcgtggaa ctcgattcgg
//
LOCUS       DAOSIO010000243         1821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_55618_length_1821_cov_2.848811, whole genome shotgun sequence.
ACCESSION   DAOSIO010000243 DAOSIO010000000
VERSION     DAOSIO010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55618_length_1821_cov_2.848811"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PK590_07630"
     CDS             <1..328
                     /locus_tag="PK590_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24505.1"
                     /translation="GGVQYYLEQMLGYDEYKKNSGSTRDEYRHVVTLRLTRLLMNQNL
                     TLSFFGYYSPSDKDAYLRPHVHYKASDHLGFEVGSNIFFGDYRNTFFGQFRDNTHLYT
                     AVRYSF"
     gene            442..927
                     /locus_tag="PK590_07635"
     CDS             442..927
                     /locus_tag="PK590_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOG24506.1"
                     /translation="MTIKRMPYSLDFFRDVCKSNGMRVTRQKIAIYKELMKSDNHPTP
                     EQILNRLKKDFSGLSLDTVYRTLCSFTEIGLIHQVEGFGTARRFDPKLEKHHHFQCRK
                     CRAIIDFTSEDYDHIEIPKDISRDCEVLNLRVTIEGYCKKCCIQQKEKKGAVLWKKRK
                     N"
     gene            906..>1821
                     /locus_tag="PK590_07640"
     CDS             906..>1821
                     /locus_tag="PK590_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003103909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HOG24507.1"
                     /translation="MEEKKKLTTNAGCPVPDNQNVMTAGPRGPQLLQDVWFLEKLAHF
                     DREVIPERRMHAKGSGAYGNFTVTADITRYSKAKVFSKVGKMTDVFVRFSTVAGERGA
                     ADAERDIRGFAVKFYTEQGNWDLVGNNTPVFFLRDPLKFPDLNHAVKRDPRTNLRSAL
                     NNWDFWTSLPEALHQVTIVMSDRGIPATYRHMHGFGSHTFSMINDKNERFWVKFHLKS
                     QQGIKNLTDEEAEKVIGKCRESHQRDLYYSIEKGDFPRWTMYIQAMPELDAEKLPYHP
                     FDLTKVWYHSDYPLIEVGVLELNRNPENY"
BASE COUNT          523 a          409 c          434 g          455 t
ORIGIN      
        1 aggaggggtg cagtattacc tggagcagat gctcggatat gacgagtata agaaaaactc
       61 cggttctacg cgggatgaat atcgtcacgt ggtcacgctg cgattgacac ggctgctgat
      121 gaatcaaaac ctgacgttgt catttttcgg gtattattcc ccttcggaca aggatgccta
      181 tctgcggccg catgtacatt acaaggccag cgatcatctg ggctttgagg tcggctccaa
      241 tatcttcttc ggcgattatc gaaatacctt cttcggtcag tttcgggata atacccattt
      301 gtacacggcc gtgcgctata gtttttaaaa tacggcaaaa atacacactt tgcttgcgga
      361 aaaagagaag tccgctgcaa ttgtacaaaa attataaaag atatcttgac aactctgccc
      421 aaatttatta ttaagaatta tatgacaata aagagaatgc cgtatagtct tgattttttt
      481 cgcgatgtct gcaaatccaa tgggatgcgg gttactcgtc agaaaatcgc catttataaa
      541 gaactgatga aatccgacaa ccaccctacg cccgaacaaa tattaaacag actcaaaaaa
      601 gacttcagcg gcctttctct ggatacggtc tatcgaaccc tttgctcctt taccgaaata
      661 gggcttattc atcaggtcga aggatttgga accgcccgac ggtttgaccc gaaacttgaa
      721 aaacaccatc attttcaatg caggaagtgc cgcgccatca tcgattttac gtccgaggat
      781 tatgaccaca ttgaaattcc caaagatatt tcgcgggatt gtgaggtttt aaatctgcgc
      841 gttacgattg aaggatattg taaaaaatgc tgcatacaac agaaagaaaa gaaaggggcc
      901 gttttatgga agaaaagaaa aaactaacta ccaatgcagg gtgtcccgta cccgataacc
      961 agaatgttat gacagcgggg ccaagaggtc cgcaattgct tcaggatgtc tggtttcttg
     1021 aaaaactcgc tcattttgac cgcgaggtta tccccgaacg gcgaatgcac gccaaaggtt
     1081 ccggggcata cggcaatttt accgttacgg cggatattac ccgttacagc aaggcgaaag
     1141 ttttttccaa agtcggcaaa atgacggatg tgtttgtccg tttctcaaca gtagccggag
     1201 aacgcggcgc tgccgacgcc gagcgcgata ttcgagggtt tgccgtcaag ttttacaccg
     1261 aacagggcaa ctgggatttg gtgggcaaca acacgcctgt attttttctg cgagatcccc
     1321 tgaaatttcc cgatctcaac catgcggtca aacgagatcc tcggaccaat cttcgaagcg
     1381 ctctgaacaa ctgggatttt tggacttctc tgccggaagc gctgcatcag gtgaccattg
     1441 tgatgagcga tcgcggaatc ccggcaacat atcgccacat gcatggtttc ggaagtcata
     1501 cattcagcat gatcaacgac aaaaatgagc gattctgggt aaagtttcac ctgaagtcgc
     1561 agcagggaat taaaaacctg acggatgaag aggccgaaaa ggtcatcggc aagtgccgcg
     1621 agagccacca gagagattta tattacagta tcgaaaaagg cgatttccct cgttggacga
     1681 tgtatatcca ggcaatgccg gaactggatg ccgaaaagct gccttatcat cccttcgatt
     1741 tgaccaaggt gtggtatcac agcgattatc cactgattga ggtgggtgtt ttggaactga
     1801 atcgaaatcc ggaaaactat t
//
LOCUS       DAOSIO010000244         1813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_55958_length_1813_cov_3.412969, whole genome shotgun sequence.
ACCESSION   DAOSIO010000244 DAOSIO010000000
VERSION     DAOSIO010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55958_length_1813_cov_3.412969"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PK590_07645"
     CDS             <1..826
                     /locus_tag="PK590_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG24508.1"
                     /translation="LLDLGLEPNLIASSLAAVVAQRLVKRICPHCKEEYTPDPSELEK
                     IRSCLDKVSTPRFYRGKGCEECGFTGFFGRMPILELLEISEAIKAMIERKASANEILT
                     EARREGLKPLSTAAIEKMLEGYTTLDELARVIVLEEDEVSRMAAKRGERPRILIVDDE
                     EDILRALEKRLMMANCDVIKARDGSEGLLLAFQEKPDLVITDVTMPKMNGFELTKQLR
                     SSLETAVIPIIMLTARHDKESELKGIDAGADDYIIKPFDHDKLLARLRMLLRRTSF"
     gene            854..>1813
                     /locus_tag="PK590_07650"
     CDS             854..>1813
                     /locus_tag="PK590_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOG24509.1"
                     /translation="MLFTYQAVTAQGEKVKGTIEAVDRKDAGVKLNEKGLQPVAIQES
                     VLPHSRLFSPLFGKVRSDELLIFYSQLYDMVSAGINFLKSLEMISEQTRNRKLKNAID
                     DIIALLRKGTSISDAFDQHKDIFPPLFVSMIKSGEASGELETVLRNYNVLYDEHLEIQ
                     RKIAGALFYPVVLFCLGMAVILFLVVFIVPKFVEIFSTAGIALPMITQVLYVFGTSVI
                     RYWYLILAVTGLVAWGIWRYLKTGPGRYHVDSLKLMIPVFGPLNRNVAFSRFLTTMGL
                     LLKSGVSIIKALELASDVMQNVVLQSSMAEFPERMRRGETLSAM"
BASE COUNT          452 a          391 c          502 g          468 t
ORIGIN      
        1 gcttcttgat ctcgggctgg agcctaactt aatcgcgtca tcgttggccg ctgttgtggc
       61 ccaaagactt gttaagagaa tatgtcccca ttgcaaggaa gaatatacgc cggatccgtc
      121 tgaacttgaa aaaatcagaa gttgtctgga caaagtcagc acgccccggt tttaccgggg
      181 aaaaggttgt gaggaatgcg gttttacggg atttttcggg cggatgccga tcctggagct
      241 tttggaaatt tcggaagcga tcaaggccat gatcgaacgc aaagcttccg ccaacgagat
      301 tcttactgag gccaggaggg aagggttgaa accgttatca accgccgcga ttgagaaaat
      361 gcttgaaggg tacaccaccc tggatgaact tgcccgggtc attgtccttg aagaggacga
      421 ggtaagtcgc atggcggcca aacgtggcga acgaccccgg atcctgatcg tggacgatga
      481 agaggatatt ctgcgggcgt tggagaaacg gcttatgatg gccaactgcg acgtgattaa
      541 ggcgagggac gggagcgagg ggcttcttct ggcttttcag gagaagccag atcttgtcat
      601 taccgacgtt acgatgccga agatgaacgg atttgagttg acgaagcagc ttcgttcgag
      661 tctggagacc gccgttatcc cgatcatcat gctgaccgcg cggcatgaca aggaaagtga
      721 gttgaaaggg attgatgccg gagcggatga ctacattata aaaccgttcg accatgataa
      781 gcttttggca aggctaagaa tgcttttgag aaggacttct ttttgagact ctccgtgaga
      841 tgcgaggact agaatgcttt ttacttatca ggcagtgacc gcccaagggg aaaaagtcaa
      901 gggaaccatt gaggctgttg acagaaagga tgctggggta aaactcaatg aaaaggggct
      961 ccagcccgtc gcgattcaag aatcggttct gccccattca aggcttttta gccctctttt
     1021 tggaaaagtg cggtccgatg aattgctcat cttttattcg caactttacg acatggtgag
     1081 cgccgggatc aatttcttaa aaagtcttga aatgatctcg gaacaaacgc ggaaccgaaa
     1141 actgaaaaac gcgattgacg acatcatcgc ccttctcagg aaaggcacaa gtatctccga
     1201 cgcgtttgat caacataagg acattttccc gccgctgttt gtcagcatga tcaaaagcgg
     1261 ggaagccagc ggagagttgg agactgtgct tcggaattac aatgttttgt acgacgaaca
     1321 tcttgaaatt cagcgtaaaa tagcgggcgc ccttttttat cccgtggtac ttttttgttt
     1381 gggtatggcg gtgattttgt tccttgtggt ttttatcgtt cctaaattcg ttgaaatctt
     1441 ctcgacagcc gggattgcct tgccgatgat cactcaggtg ttgtatgtgt ttggaacttc
     1501 ggtgatccgc tactggtatt taatccttgc cgttacgggt ttggtcgcct ggggaatctg
     1561 gcgttatctc aagacaggcc cgggcagata tcatgtcgat tccttgaagc ttatgatccc
     1621 tgtttttggt cccctgaatc gaaatgtcgc tttctcgagg ttcctgacca cgatgggatt
     1681 acttttgaaa agcggggttt ccattatcaa ggctctggaa ctggcgagcg acgtgatgca
     1741 aaacgttgtc ttacaaagct ccatggcgga atttccggaa agaatgagaa gaggagagac
     1801 cctctcggcc atg
//
LOCUS       DAOSIO010000245         1811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_56049_length_1811_cov_3.089408, whole genome shotgun sequence.
ACCESSION   DAOSIO010000245 DAOSIO010000000
VERSION     DAOSIO010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56049_length_1811_cov_3.089408"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="PK590_07655"
     CDS             <1..1012
                     /locus_tag="PK590_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOG24510.1"
                     /translation="EELHALQDMAKAVGTLDALMSFAHIAIEKKWVAPEVNEGFGILI
                     EGGRHPVVESMLPTGKFVENDTMIDRSKEQLIILTGPNMAGKSTFIRQVAQIVLLAQM
                     GSFVPARKAVIGLVDRIFTRIGASDDLARGESTFMVEMLETAHILHEATDRSLLILDE
                     VGRGTSTFDGVSIAWAMCEFLVQGDRRPRTLFATHYHELTQLKEHFAWIRNYKMAVKE
                     TQDGVLFLRKVIRGASERSYGIHVAELAGLPAEVTTRAKEILAILESGNTEATAIIES
                     GKVKKRKSGAEKFSASSNSIEKKPNEVEEALKKMDVDRMTPIEALQKIAELKERLEKP
                     KR"
     gene            1123..>1811
                     /gene="mutL"
                     /locus_tag="PK590_07660"
     CDS             1123..>1811
                     /gene="mutL"
                     /locus_tag="PK590_07660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOG24511.1"
                     /translation="MGKIRVLPEQVANKIAAGEVVERPSAIVKELTENSLDAGASQIE
                     VCVRHGGKSFIRVTDNGAGMSAEDAQISVQRHATSKIEKAEDLDEIRSFGFRGEALSS
                     IAAVSRFTLQSRAEGADIGIELVIEGGSLVKAKECSVARGTTVEVRDLFFNTPARRRF
                     LRQDSTEFGHILDAVTCLALANLNVHFTLISQEQVVLDLIPTEDLPVRARAIFGDHWA
                     DRTLLVRGDIP"
BASE COUNT          443 a          382 c          543 g          443 t
ORIGIN      
        1 tgaggaactg cacgcgcttc aggatatggc caaggcggtt ggcacgctgg acgcgctgat
       61 gtcgtttgct cacattgcga ttgaaaagaa gtgggtcgcg ccggaagtga acgaagggtt
      121 tgggattttg atcgagggag gccgtcatcc ggtcgtggag agtatgcttc ccaccggaaa
      181 gtttgttgaa aacgatacca tgatagaccg ttccaaggag cagttgatca ttttgactgg
      241 gcccaacatg gccgggaaat ctaccttcat tcgtcaggtc gcccagattg tgcttcttgc
      301 ccaaatgggg tcctttgtac cggcccgaaa agcggtaatt ggacttgtcg atcggatttt
      361 tacgcgaatc ggcgcctcgg atgatcttgc gcgcggagaa agcacattca tggttgagat
      421 gcttgagacg gctcatatcc tgcatgaggc gacggatcgt agcctgctca ttcttgatga
      481 ggtgggacgt ggcacttcga cctttgacgg ggtcagcatt gcgtgggcga tgtgcgaatt
      541 tcttgtccaa ggggacagac gacctcggac gctttttgcc acgcactatc acgaacttac
      601 ccagctcaag gagcattttg cctggatccg gaattataaa atggccgtta aggaaaccca
      661 ggatggggtc ttatttttaa ggaaagttat ccggggtgcc tcggaacgta gttatgggat
      721 tcatgtggcg gagctggcag gtcttccggc cgaagtcacg actcgcgcca aagaaatcct
      781 tgcgattctt gaaagtggaa atactgaagc aacggcgatt attgagtcag ggaaggttaa
      841 aaaacgcaag tcaggcgcgg aaaagttttc ggcgtcttca aactcgatcg agaaaaagcc
      901 caatgaggtt gaagaagcat tgaaaaagat ggacgttgac cggatgacgc cgatcgaggc
      961 gcttcaaaag atcgctgaat taaaagaaag acttgaaaaa ccaaagcgct agacagaagt
     1021 gaatgggttt ttaatttgaa attttacggt ggcgttattt tcttttgcga acgcgtaaag
     1081 atttcgggag tggaatggtt gttatgattt ccggaggaac gaatgggtaa aatacgtgtt
     1141 ttgccggaac aggtggcgaa caaaatcgcg gcgggcgagg tcgttgaacg accctccgct
     1201 attgtcaagg aattgacgga gaattctctg gacgcaggcg cgagccagat tgaggtttgt
     1261 gtccgtcatg gtggaaaaag ttttattcgg gtgaccgata atggggcggg aatgagcgcg
     1321 gaagacgccc agatttcggt ccagcgtcat gccacaagca agattgagaa agcggaagac
     1381 cttgacgaga tacggagttt cgggttccgg ggggaggcgc tttccagtat tgccgcggtt
     1441 tcaaggttta cgcttcaaag tcgagccgag ggagcggaca tcgggatcga gcttgtcatt
     1501 gaaggcggct ctctcgtgaa agcaaaagag tgttcggtcg cgaggggaac gacggtcgag
     1561 gttcgagatc ttttttttaa cacaccggcg agaagaagat ttcttcgtca agactcgacc
     1621 gagttcgggc atatccttga cgcggtgact tgtcttgccc ttgccaattt aaatgtccat
     1681 tttacgctta tttcgcagga acaagtggtt cttgatctga ttcccaccga agatcttcct
     1741 gtccgggcgc gcgctatttt tggcgatcac tgggcagatc gaacgctttt ggtgcgcgga
     1801 gatatccctt a
//
LOCUS       DAOSIO010000246         1801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_56463_length_1801_cov_3.265750, whole genome shotgun sequence.
ACCESSION   DAOSIO010000246 DAOSIO010000000
VERSION     DAOSIO010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56463_length_1801_cov_3.265750"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="PK590_07665"
     CDS             complement(<1..862)
                     /locus_tag="PK590_07665"
                     /inference="COORDINATES: protein motif:HMM:NF028240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HOG24512.1"
                     /translation="MKKTVGRIMSLLMIFSFAVGQTAFADEAQLMSLLEQMQNQMSEL
                     QKTVAAQKTEIQALKTRGPSIEVAPSGVESPKINEDDFKKQFDKNLKDKIGDSDKWLK
                     DLKAKGDIRMRYEPANNTRATSADVNRFRFRLRWGLEKKFNDEFKAGFRLASGGTTDP
                     TSTNQSFDSAFANKAITIDQAYAIYNPEWARMGPICDVEIGVGKVANPLSDVHSWLSW
                     DSDVTPEGIYEKVTSRLFKTENLSTDVEFLGTQFVINESSRRSNVDGDAEFFGFSGGI
                     RNKFTHENLQH"
     gene            complement(1098..1610)
                     /locus_tag="PK590_07670"
     CDS             complement(1098..1610)
                     /locus_tag="PK590_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24513.1"
                     /translation="MKRLLLIASLVFLNVSPGFAETGVVKPLRTDLILEVVPIKTRSI
                     LAGGKVDALEKSPSEMPPVWTAVVFRVNRVLMGSFKSVIIEEPPLREQMKTAVKEKKI
                     WKFLTMDFKKTDEEGIPRKWLTLAVADPYASFGIKEGEPSPRQRYKLYLSLAHKNPDS
                     YVLVKSEKIV"
BASE COUNT          441 a          468 c          415 g          477 t
ORIGIN      
        1 gatgctggag attctcatgg gtgaatttat tgcggattcc ccccgaaaaa ccgaaaaact
       61 cagcatcacc gtcgacgttg ctcctcctgg acgattcatt gatgacgaac tgtgtcccga
      121 gaaattcaac atccgtgctc aaattttcag tcttgaacag gcgggaagta accttttcat
      181 aaataccttc cggcgtcacg tcgctgtccc agctcagcca gctgtgaacg tccgagagcg
      241 gattcgccac tttacccacg ccaatctcaa catcgcagat gggacccatt cgcgcccatt
      301 cgggattgta aatcgcgtaa gcctgatcaa tcgttatggc tttattggca aaagcgctgt
      361 caaaactctg gttcgtggac gtgggatctg tcgtaccacc tgaagcaagc cgaaaccccg
      421 ccttgaactc atcattgaat ttcttctcaa gtccccagcg caaacgaaac cggaaccggt
      481 ttacgtccgc cgatgtcgcc ctcgtattat tcgcgggctc ataacgcatg cgaatgtcgc
      541 ctttcgcctt gagatcctta agccatttgt cagaatctcc gatcttgtct tttaaatttt
      601 tgtcgaattg ttttttaaaa tcatcttcat tgattttcgg cgattcgact ccgctgggcg
      661 ctacctcaat agagggcccc ctggttttta aagcctgaat ctccgtcttt tgcgcagcca
      721 cggtcttctg caattcgctc atctgatttt gcatctgttc cagcaaactc ataagctggg
      781 cttcatccgc gaaagcggtt tgacccacag caaacgagaa aatcatgagc aaactcatta
      841 ttctccctac tgttttcttc atggttttct tctccctttg tttaagttcc ccggaatgta
      901 ggaccattcc cgttggacct gttacgggag aagtgtacaa aagcagtgtc agatctttat
      961 gaggggcgtg taaagttatt gtaaaacctc ggccgcatta tccggcggtc atcaccatga
     1021 gcgaaggcgc gctcgtttcc gttcatgggc tgaccgggcc ggttggcgct gaagtgggac
     1081 gagagacaga aaaagagcta gacgatcttt tcgcttttga cgaggacgta gctgtccgga
     1141 ttcttgtggg caagggacag ataaagcttg tagcgttgcc ggggggaggg ctctccttct
     1201 tttattccga aagaggcata aggatcggcc acggcgaggg tcaaccattt tcggggaata
     1261 ccttcttcat ccgttttttt aaaatccatg gtaaggaact tccagatttt cttttctttc
     1321 acggccgttt tcatctgctc gcggagagga ggttcttcaa taataacgct tttaaaactc
     1381 cccatcagaa cccggttgac cctgaaaacc acggccgtcc agacgggggg catttcagaa
     1441 ggcgattttt caagcgcgtc aaccttcccc cctgccagga tactacgcgt cttgatggga
     1501 accacttcaa ggataagatc cgttcgcaga ggtttcacca cgcccgtctc ggcaaaaccc
     1561 ggggaaacat ttaaaaacac aagactagca atcagcagga gccttttcac agtatccctt
     1621 tcttttgttg ccggcagtat aaacaatccc gaaaacctct catgtaaaaa ctacgtaaaa
     1681 tccgccttcg gaaaatgtct ccggcaaaac ctggcctggt tccggaagac agaataaacg
     1741 aaagggtcta gaagcggttt cataagttgt tgcaagtgat taacccacaa gcgatatgga
     1801 g
//
LOCUS       DAOSIO010000247         1800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_56511_length_1800_cov_3.318052, whole genome shotgun sequence.
ACCESSION   DAOSIO010000247 DAOSIO010000000
VERSION     DAOSIO010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56511_length_1800_cov_3.318052"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /gene="argB"
                     /locus_tag="PK590_07675"
     CDS             complement(<1..330)
                     /gene="argB"
                     /locus_tag="PK590_07675"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOG24514.1"
                     /translation="MKQLIEKANVLIEALPYIKKFRGKEVLIKYGGAALTYQEVRDKV
                     LQDLVFMSYVGIRPILIHGGGKFITENMKKKGIQSQFVNGLRVTDKPAIAVVHDTLAE
                     LNRSIVRD"
     gene            complement(421..1644)
                     /gene="argJ"
                     /locus_tag="PK590_07680"
     CDS             complement(421..1644)
                     /gene="argJ"
                     /locus_tag="PK590_07680"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOG24515.1"
                     /translation="MMKKTFWKKEKNGSVTSPIGFVAGGIHAGIKKKKKKYDVSLIFS
                     KTPCVAAGTFTTNRAKAWPVLYSMKVIDGSRHHAIIGTSGNANCLNGIIGKKAVDEIT
                     WETAKHLDILPQEVLIAQTGIIGVPFPVTRVKRAIPKLVARLSEKGGHGAAKGILTTD
                     LSNKEIAISLELGGKKVTIAACAKGAGMVHPNMATMLCFITTDCAITKSMLRRAVRHA
                     ADDTFNTMAIDNDMSTNDMVLCLANGLARNPTITKIGKDYWMFREALEEVMRYIAHEM
                     VKDGEGVTHVCHLRLSGAKTPTDAERVARQIANSMLFKTMLAGSNPNWGRIAAAVGAS
                     GVDFNPNHLSISFGDAEVLHQGKLRVLNIPKARRILQKKEYSIFVKIGKGRGRADFIT
                     SDLTTKYVLINAAYS"
     gene            complement(1645..>1800)
                     /gene="argC"
                     /locus_tag="PK590_07685"
     CDS             complement(1645..>1800)
                     /gene="argC"
                     /locus_tag="PK590_07685"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943503.1"
                     /note="catalyzes the reduction of N-acetyl-5-glutamyl
                     phosphate to N-acetyl-L-glutamate 5-semialdehyde in
                     arginine biosynthesis and the reduction of
                     N-acetyl-gamma-aminoadipyl-phosphate to
                     N-acetyl-L-aminoadipate-semialdehyde in lysine
                     biosynthesis; involved in both the arginine and lysine
                     biosynthetic pathways; lysine is produced via the AAA
                     pathway, lysine from alpha-aminoadipate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOG24516.1"
                     /translation="TNFCDIGVSLDSRTGRLVILTAIDNLLKGASGQAVQNMNIRCGF
                     PEGAGFV"
BASE COUNT          406 a          514 c          399 g          481 t
ORIGIN      
        1 atcgcgcaca atactcctgt tcaactcggc taaagtatca tgaaccaccg cgatcgcggg
       61 cttatccgtc acgcgaagac cattgacaaa ttggctctgg attccttttt tcttcatgtt
      121 ttcggtgata aatttcccgc cgccgtgaat taaaatgggg cgtatcccga cataactcat
      181 aaaaacaaga tcctggagca ccttgtcgcg cacttcctgg taggtgagcg cggcgcctcc
      241 atacttgatc aaaacttctt tcccgcgaaa tttcttgata tagggaagcg cctcaatcag
      301 aacgttggct ttttcgataa gttgcttcat ctttgctaac gtgacaggat ccagtgttca
      361 gggcttaaaa aaatcctttc gaatttttat tccctatccc ctgctcctat cccctttctt
      421 ttaggaatat gcggcgttga ttaaaacata tttcgtcgtc aaatcagacg ttataaaatc
      481 cgcccggcct cgcccttttc cgattttgac gaaaatcgaa tactctttct tttggagaat
      541 ccgtcgcgcc ttcgggatat tcaggacccg cagtttcccc tggtgcaaaa cctcggcgtc
      601 accgaacgaa atgctcaaat gattcgggtt aaaatcgact ccgctcgccc ccacggccgc
      661 cgcaatccgg ccccagttag gattggaacc cgccagcata gtcttgaaaa gcatcgagtt
      721 cgcaatctgt cgggcaaccc gctcggcatc cgtcggagtc ttggcgccgg aaagtcgaag
      781 gtggcacaca tgagtcacgc cttctccgtc tttcaccatt tcgtgggcga tgtaacgcat
      841 gacttcctca agcgcttccc gaaacatcca gtaatccttg ccgattttgg tgatcgtggg
      901 atttcgcgcc aatccgttgg caaggcaaag aaccatatcg ttggtgctca tgtcattatc
      961 gatcgccatg gtgttgaagg tgtcatcagc cgcgtgccga accgcccgcc gcaacatgga
     1021 ctttgtaatc gcgcaatccg tcgtgataaa gcaaagcatc gtcgccatat tcggatgcac
     1081 catccccgcc cctttggcgc aggccgcgat cgtcactttt ttcccgccca actcgagcga
     1141 aatcgctatc tccttgtttg aaagatccgt cgtcaaaatc cccttggcgg ctccgtgccc
     1201 ccctttttcg gaaagcctcg cgaccagttt cgggatggcg cgtttgaccc gtgtgacggg
     1261 aaacgggaca ccaataatcc cggtctgggc tatcagtact tcctgcggca gaatatcgag
     1321 atgttttgcg gtctcccagg taatttcatc gactgctttt ttcccgatta ttccgttcag
     1381 gcaattggca ttcccactcg ttccgatgat ggcatgatgt cgtgaaccat cgatcacttt
     1441 cattgagtag agaaccggcc acgccttggc gcggttagtg gtgaacgtcc cagccgcgac
     1501 gcagggagtc tttgaaaaga tcagtgacac gtcgtatttc tttttcttct ttttgatccc
     1561 tgcgtgaatt cctcccgcca caaatccgat aggcgacgta accgaaccat ttttttcttt
     1621 tttccaaaaa gtttttttca tcattcagac aaaccccgct ccctcgggga aaccacaacg
     1681 aatgttcatg ttttgtaccg cctgccctga agcgcctttc aaaagattat caatagccgt
     1741 caaaatcacc agccgccccg tgcgtgaatc cagggaaaca ccgatgtcgc aaaagttcgt
//
LOCUS       DAOSIO010000248         1780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_57434_length_1780_cov_3.947246, whole genome shotgun sequence.
ACCESSION   DAOSIO010000248 DAOSIO010000000
VERSION     DAOSIO010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57434_length_1780_cov_3.947246"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PK590_07690"
     CDS             complement(<1..193)
                     /locus_tag="PK590_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24517.1"
                     /translation="MKKQSVLLTAVLRRLSLHCFPKGWEAGRGLLKSLLIPAFISSFF
                     LSPVAFAAEVYYLGQSTSPD"
     gene            complement(532..923)
                     /gene="ssrA"
                     /locus_tag="PK590_07695"
     tmRNA           complement(532..923)
                     /gene="ssrA"
                     /locus_tag="PK590_07695"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1031..1549)
                     /gene="smpB"
                     /locus_tag="PK590_07700"
     CDS             complement(1031..1549)
                     /gene="smpB"
                     /locus_tag="PK590_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOG24518.1"
                     /translation="MKRNNLDKTKPAESSQKKRRTFEPIAENRKAGFNYFLSDKREAG
                     ICLLGHEVKSIREHRVNLKDSYVRIFKNEAWLFNCHISPYSHIQGHLEQDPTRMRKLL
                     LNRREIDDLMGKSSRKGFAIIPTRLYFKNGMVKVEIALGEGKKQRDKRDSIKKRIHDR
                     ETSAAIKKGMRK"
BASE COUNT          460 a          464 c          433 g          423 t
ORIGIN      
        1 catccggcga cgtggactga cccaggtagt aaacttccgc ggcaaaagcc accggggaaa
       61 ggaagaaaga cgagatgaag gccggaatga ggagcgactt caaaagtccg cgacctgctt
      121 cccaaccctt cgggaaacaa tggagcgaaa gccgtcgtaa aaccgccgtg agtaagacgc
      181 tctgcttttt cataacccct gaggtttttg aggattggga acagcgcaac ggccgagatt
      241 atatattgaa acattatttc gtcaaaccac tttagcaaaa aatcaggaga tcaaaatcat
      301 caaaagcttt aaccgatcga ttccaacacc ccggattcgg tttgtcatac cttcaaccga
      361 aggatcgcgc gagagcttgt gaggcctcac gagtgggccg ggcgcaggga taagaaagcg
      421 accatgcggt acgcacattc agcatcggga tacagccccg aaattgagaa aatcaacctc
      481 gggaccgcta ccgattctgc taccgcctga ttttcgctct tgatacaaaa ttggtggagg
      541 tggagggaat tgcaccctcg tccaaaagat ccgtcatcaa aggttctcca tgctcagtcc
      601 ccatttcgtt taattcccca aacccatgcg gacaggaact tgaggaacga tccgtttagg
      661 tcttttcccc cggcaaaacg gacatgccgg aagagcatcc cgctaatgtc gtcagcggag
      721 cttagcgggc gtgggccccg tgacgtcgct agtccttaga cagcgagagg aacgaacaca
      781 ggagccgaaa tcgggcccac tgctctttcg ttccagctga gtttttcagc atttgtagtt
      841 tgccacgttg atttaagagg aacctgacga acctctgcat gctcccttgt atgcctgatc
      901 ccctgtcgaa cctattcacc cccaggagac tatgtaaaag aaccaggaga attatagcac
      961 agaaagatca ttggctgaat tgcacccccg tcccccgtga ggaacgggct cgcccccaac
     1021 tcagggtggg ttacttccgc atcccctttt tgatcgccgc ggatgtttct cgatcatgga
     1081 tgcgcttttt gatcgaatct cgcttgtcgc gctgtttttt cccctcgcca agcgcgatct
     1141 ccactttcac cattccgttt ttaaaataca gccgcgtggg aataatcgcg aatcccttgc
     1201 gcgatgactt gcccatcaga tcatcaattt cccgccgatt gagaagcaat ttccgcatgc
     1261 gcgtggggtc ttgctccagg tgaccctgga tatgggaata gggagaaatg tgacaattaa
     1321 aaagccaggc ttcattttta aaaatgcgga cgtagctgtc tttcagattg acccggtgtt
     1381 cccgtatcga tttcacctca tgccccagaa ggcaaatgcc tgcctcccgt ttatcggaaa
     1441 gaaaataatt aaatccggcc ttacgattct cggcgatcgg ttcaaaagta cgccgcttct
     1501 tttgcgaaga ttcagccggt tttgtcttgt cgagattgtt tcttttcata gttttcatat
     1561 gaatggagaa atcatattca agatcggagc gatcgacaag ccccaaaaaa cttgttccct
     1621 gggcaaggtc gcggtttgta gcgtacaaag aaacgccgcg gaacttgcgg actccccaaa
     1681 acggaaaaag tccgacggaa taaaccgccg ggcttttgag aaatttcgct gcggattaaa
     1741 tcttcgcaac aggagaactt gtttggggaa gatcctattc
//
LOCUS       DAOSIO010000249         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_57677_length_1775_cov_3.514535, whole genome shotgun sequence.
ACCESSION   DAOSIO010000249 DAOSIO010000000
VERSION     DAOSIO010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57677_length_1775_cov_3.514535"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /gene="hisD"
                     /locus_tag="PK590_07705"
     CDS             complement(<1..913)
                     /gene="hisD"
                     /locus_tag="PK590_07705"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOG24519.1"
                     /translation="MQVINWDKQAQKKIRRDYASYFDTKIFKKVSRILAEVRQSGDRA
                     LRRYTREFDAVDLSLKRIAVTQSDINRAFEKIRVQFVPLLKKITENVRGYYEKELNRS
                     FEVPGQHGVCLGKQYSAHERVGIYIPAGTAPLVSSVYMTVIPAKVAGVKQIIICTPPR
                     RDTGDIDPHILAVANLLGVNEIYRVGGVQAIGAMAFGTKTISKVDKIVGPGNAYVTEA
                     KRQVYGFVDIDMVAGPSEVAIIADQTANPDFVTCDLLAQTEHNGGIGYLISTSKKLTE
                     LMRSRIDNGNMIQVKSLAEACEVANEIA"
     gene            complement(916..>1775)
                     /locus_tag="PK590_07710"
     CDS             complement(916..>1775)
                     /locus_tag="PK590_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOG24520.1"
                     /translation="RFDYSFGRSLEPQEIAAIETTVNERILRDQEVHKFEKKLEQAKK
                     EGALAFFGDKYGDTVRIIDVPDFSKELCGGTHCSRTGEIGLFLILSESSVASGVRRIE
                     ATTGLGALRHVHSLQKQLAEVARMLKVGTADIPLRLAKLQETVKKQKNSGSQAKTSDF
                     DIKQIKTNVKKLRGHEVLVQWIQDSDISVLRNISDQLRGSGVRMIFVIASAREGKLSI
                     LAGRSNDLKNSSFDMKVLFGRLSELLKVTGGGRSDFVQGGGIDHGQFLAERDQIENLI
                     EHYLIEQGF"
BASE COUNT          422 a          460 c          411 g          482 t
ORIGIN      
        1 ccgcaatttc attggccacc tcacaggcct cggcgagcga tttgacctga atcatgttgc
       61 cgttgtcgat gcgggaacgc atgagttccg tgagtttctt ggaagtggaa atgagataac
      121 cgattccccc attatgctcg gtttgggcca gtaaatcgca agtaacaaag tcagggttgg
      181 cggtctgatc cgcgatgatg gcgacttcac tgggccccgc caccatatca atatcgacaa
      241 acccgtacac ttggcgtttg gcttccgtga cataggcgtt tcctgggccc acgatcttgt
      301 cgactttgga aattgttttg gtcccaaaag ccattgcccc gatggcctgg acaccgccca
      361 cccggtagat ttcattgacg cccagcagat ttgccaccgc cagaatgtga ggatcgatgt
      421 ctcccgtgtc gcgccggggc ggagtacaaa taatgatctg tttgactccg gcgaccttgg
      481 cagggatgac cgtcatataa acggaggaaa caaggggcgc ggtgcccgcc ggaatataaa
      541 tccccacccg ctcgtgcgcc gaatattgtt ttccgagaca aactccgtgt tgcccgggaa
      601 cctcaaaaga acggtttagt tctttttcat aatagcccct gacattttcc gtaatttttt
      661 ttaacagagg aacgaattga acccgaattt tctcgaaagc gcgattaatg tcactttgcg
      721 ttacagcaat tctcttgaga gaaagatcca ccgcgtcaaa ttcccgggtg tagcggcgca
      781 aagcccgatc tcccgattgg cgaacttcag ccagaatgcg gctcaccttt ttaaaaatct
      841 tggtgtcgaa ataggacgcg taatcgcgac ggattttttt ttgcgcctgt ttatcccaat
      901 taatcacttg cataattaaa aaccctgttc gataagataa tgttcaatga ggttttcgat
      961 ctgatcgcgt tcagcgagaa actgaccgtg atcaattccc ccgccctgaa caaaatcaga
     1021 ccgccctccc ccggttactt ttaagagttc ggatagccta ccaaaaagaa ccttcatatc
     1081 aaaggatgaa ttcttgagat cgtttgatcg tcccgccaga atgcttagtt ttccttcccg
     1141 ggcagaagcg atcacaaaga tcatccggac accgctaccg cggagctgat cggaaatgtt
     1201 tctgagaacg ctgatgtctg aatcctggat ccattgaacc agaacctcgt gacctctcag
     1261 tttttttaca tttgtcttta tctgttttat gtcaaaatcg ctcgtcttgg cctgagatcc
     1321 cgagtttttt tgtttcttaa ccgtttcttg gagttttgcg agacgtagcg ggatatcagc
     1381 agttcccact ttaagcattc tcgcgacttc ggcaagttgc ttttgtaggg agtggacatg
     1441 tcgtaacgct ccgaggccgg tcgtcgcttc aatgcgtcga actccgcttg ccacggaaga
     1501 ttccgagagg atcagaaaaa gcccgatctc tccggttctc gagcagtggg ttcccccaca
     1561 aagctcctta ctgaaatcgg gcacatcaat tattcggacc gtatctccgt atttatcacc
     1621 aaagaaagca agagctcctt cttttttagc ctgctcgagt tttttctcga atttgtgaac
     1681 ttcttgatct cggaggatac gttcattcac cgtcgtttca atcgcggcaa tttcctgggg
     1741 ttctagcgag cgaccaaacg aatagtcaaa cctca
//
LOCUS       DAOSIO010000250         1750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_58765_length_1750_cov_5.250737, whole genome shotgun sequence.
ACCESSION   DAOSIO010000250 DAOSIO010000000
VERSION     DAOSIO010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58765_length_1750_cov_5.250737"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..1050
                     /locus_tag="PK590_07715"
     CDS             106..1050
                     /locus_tag="PK590_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOG24521.1"
                     /translation="MKNKNYDKKIFRLVSILNKLNGGGKVRSSELAEEFNVSIRSVQR
                     DLDLLNLTGFPLDTPEKGIYSFMPGFSLRKLMLTDEEASLLTFFHEIADSLGKNFSES
                     FRSLVSKVLAREDSSPFFVKLPEIPAHMKNFPFVKELEQAVNESQKILIKYESANGIK
                     EHQLCPLKLVNFEGFWYLLAMPDGKRQFRKFRIEKIKGLEFVDESFDPPRNLKAALER
                     SVNIWFSEEPGETILLEIDKDSAHYFKKKIYFPEQRIIKQKKDGTLILQTQANHNEVL
                     RTIIQWIPDIRVIKPKELDLKVRHLLNAYLKGSRPRWG"
     gene            1262..1624
                     /locus_tag="PK590_07720"
     CDS             1262..1624
                     /locus_tag="PK590_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOG24522.1"
                     /translation="MLAASLAGCATLFKSKMKSVSFDSDPQGAEIFINGNRMGTTPMP
                     MNFSNKKTLTITFKKPGYEDKTFIVNTKIGAGWVILDCLGGFIPVIIDAVTENWYSLE
                     TDEVKVLLDVRQAHAGSL"
BASE COUNT          488 a          400 c          436 g          426 t
ORIGIN      
        1 ctgcgccggt tttatagccg cgtagcctga agacagagac tggaatctcg caattggctc
       61 aaaaagcgac atgatgctgt cgcgtcttat gctatacttc cgcttgtgaa aaacaaaaac
      121 tacgataaga agatattccg actggtgtcg atcctgaaca agctcaacgg gggcgggaag
      181 gtgcgctcca gtgaactggc cgaagagttc aatgtttcca ttcgtagcgt gcagagggat
      241 cttgatctcc tgaacctgac gggatttccg cttgatacgc ctgagaaggg gatctacagc
      301 tttatgccgg ggttttcgct ccggaagtta atgcttaccg atgaagaagc ttcgcttttg
      361 acctttttcc atgaaattgc ggattcgctc ggaaaaaact tctcggagtc tttccggagc
      421 cttgtgagca aagtcctcgc ccgcgaagat tcctcgccct tttttgtcaa attgcccgag
      481 attccggcac acatgaagaa tttcccattc gttaaggaac tggagcaggc ggtcaacgag
      541 tcacagaaga tcctgatcaa atatgaatcc gcgaatggta ttaaagaaca ccagttgtgc
      601 ccgctcaaat tggtcaactt tgaggggttc tggtacctgc ttgccatgcc ggacgggaag
      661 cgtcaattcc gaaagttccg gatagaaaag atcaaaggac ttgagtttgt ggatgagagt
      721 tttgatccac cgcggaattt gaaagcggcc ctcgaacgga gcgtgaatat atggtttagt
      781 gaggagccgg gcgaaacgat cctgcttgag atcgataagg actctgccca ctattttaag
      841 aagaagatct atttcccgga gcaaaggatt atcaaacaga aaaaagacgg aacgcttatc
      901 ctgcagacac aggcgaatca caacgaagtc cttcgcacca tcatccagtg gatcccggac
      961 atccgagtca tcaagcccaa agagcttgac ttgaaggttc gccatcttct gaacgcatat
     1021 ctcaaaggct cccgtcctcg ctggggataa gcctctttcc ccgctcctag ccctagtatt
     1081 gccgaaaaaa tagcccacaa aatatatgcg acataatgct gtcgcgctcc catattaaac
     1141 tctttgccat gagaaagatc gcccaagaaa agccgcgtgt ttttaggaag aaaaagagac
     1201 aacaaaaaag ggggagttca tgacgtgttt taaaaaaatc acatcaatag cgttgtgttt
     1261 aatgctggca gcgtcacttg ccggttgcgc cacattgttt aaaagcaaaa tgaaatcagt
     1321 cagctttgac tcggacccgc agggagcgga gatctttatc aatggcaacc gcatgggtac
     1381 cacgccgatg ccgatgaatt tttccaacaa gaagaccctg acgatcacat tcaagaagcc
     1441 cggctatgaa gacaagacat ttattgtcaa caccaagatc ggtgcgggtt gggtcattct
     1501 ggactgcttg gggggattta ttccggtgat catcgatgct gtgaccgaga attggtatag
     1561 cttggagacg gatgaggtga aagttctttt ggatgtacga caggcgcatg cgggctctct
     1621 ttaaaagaag gaaactatta tgaagacaat attgatatta ctttttatct taacagctcc
     1681 aagggtttca attgcagaca gcgactacac cttggcgccg aacgggaagt atgtcagcga
     1741 tggcaatcct
//
LOCUS       DAOSIO010000251         1736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_59469_length_1736_cov_3.625818, whole genome shotgun sequence.
ACCESSION   DAOSIO010000251 DAOSIO010000000
VERSION     DAOSIO010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59469_length_1736_cov_3.625818"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..623
                     /gene="rplM"
                     /locus_tag="PK590_07725"
     CDS             192..623
                     /gene="rplM"
                     /locus_tag="PK590_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOG24523.1"
                     /translation="MKTFIPKDKDINRKTYLIDASGKTLGRLATRAASLLIGKGKTCF
                     SKDQLVGDQVIVINAAKIHVTGKKKEQKVYTRYSGFAGGMRSIPLEELLERKPADVIT
                     KAVTRMLPKNRMGDVMAKRLRVFPGAEYNHQAQKPITIEVS"
     gene            626..1018
                     /gene="rpsI"
                     /locus_tag="PK590_07730"
     CDS             626..1018
                     /gene="rpsI"
                     /locus_tag="PK590_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018867174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOG24524.1"
                     /translation="MATTSIYATGRRKSAIARVWLFPGQKGFTVNGQASDKYLKRANL
                     QMIVEQPLKAMNLLGQVRVRARVEGGGTAGQAGAIRLGIARALLGLNAESRSPLRRGG
                     YLTRDPREKERKKAGRKGARRRFQYTKR"
     gene            1315..>1736
                     /locus_tag="PK590_07735"
     CDS             1315..>1736
                     /locus_tag="PK590_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24525.1"
                     /translation="MTEGSVFANPSPVGKTKTWKKGFRFFLFLFFALLIYFGFVWFQA
                     NRLLRSEKNQKHFIEKIQLVSGGVFTHEGLKVGFSPNPTLTFLNPKLTFADHPTVIES
                     EKLCLDFNIISIAWGRTEPSEVYAFGVKLHTSIPAFPGL"
BASE COUNT          430 a          399 c          427 g          480 t
ORIGIN      
        1 tggtatactt tgcgcgcttt ttcagggtac ttcgagtcac aggaagatag tcctcctagc
       61 tgatttcccc ctaatatgtt tatataaaac aaagaaagga tgcctcgttg ttttcttccg
      121 gaaacagcaa aagaggctgg tttcggaagg tctgttgtct ccaatgacag aatcggatgg
      181 ggccgtcgtt tatgaaaact tttattccta aagataaaga cattaatcgc aaaacttacc
      241 ttattgacgc gtccggcaag acccttggcc gtttggcaac ccgggccgcc tctcttctca
      301 tcgggaaggg gaaaacttgt ttttccaaag accagctagt gggagatcag gtgattgtga
      361 tcaatgccgc gaaaattcac gtgacgggga aaaagaaaga gcaaaaagtt tatacccggt
      421 attcgggatt tgcgggtggc atgcgtagta tcccccttga agagttgtta gaaagaaaac
      481 cggcggatgt tattaccaag gccgtaaccc ggatgcttcc caagaaccga atgggggatg
      541 tcatggcgaa gcgccttcgc gttttccccg gcgctgaata taatcatcaa gctcaaaaac
      601 caattacgat tgaggtttca taattatggc gaccacatct atttacgcga ccggccgaag
      661 aaaatccgcg atcgcccgtg tttggctgtt ccccggacag aaagggttca cggtcaatgg
      721 acaagcttct gacaagtatc ttaaacgcgc aaatcttcaa atgatcgtgg agcaaccgct
      781 taaggccatg aaccttctcg gacaggttcg ggttcgcgcc cgtgtcgagg gcggaggaac
      841 cgcgggacaa gcgggtgcga tacgactcgg cattgccagg gcgcttctcg gattgaatgc
      901 tgaaagtcgg tccccgttgc ggaggggtgg atacctcacg cgtgatcctc gcgagaaaga
      961 gcgtaaaaag gcaggccgca aaggtgctcg ccgccgcttt cagtacacaa agcggtaatc
     1021 cggtttttca tggagttttt tttcaaccga ggatctataa acgataggtc tcttcaggtg
     1081 cgcgttacga ccgacacttc aaacgcacct ctgtgaattt atgaaccgtc ccagtctcca
     1141 gcgtgtttgg tcgcgaaatt taatcctaat cggagtaatt tgttttacgg cctgggtgtg
     1201 ttcaggttgt cagcagtttt ttcaggatta taattattcc ccgatcagcg cttctcagtt
     1261 caatagcaat tattaagaac atccgtcttg tttgcggaag agggaccgga ttttgtgacc
     1321 gaaggatctg tttttgctaa cccatcacct gtcgggaaaa cgaagacatg gaaaaagggt
     1381 tttcgttttt ttcttttcct ttttttcgcg cttctcatct attttggatt tgtctggttt
     1441 caggctaatc ggctgttaag gtccgaaaaa aaccagaaac atttcatcga gaaaatccaa
     1501 cttgtttccg ggggggtctt cactcatgag ggtttaaaag ttggattttc cccaaatccg
     1561 actttgacgt ttctgaatcc gaagcttacc tttgccgatc atcccacggt catcgaatct
     1621 gaaaaactct gtctcgattt taatattatt tcaatcgcct ggggtcgtac ggaaccttcc
     1681 gaggtttatg cttttggtgt gaagcttcat acctcgattc ccgcctttcc aggtct
//
LOCUS       DAOSIO010000252         1725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_60019_length_1725_cov_3.495808, whole genome shotgun sequence.
ACCESSION   DAOSIO010000252 DAOSIO010000000
VERSION     DAOSIO010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60019_length_1725_cov_3.495808"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="PK590_07740"
     CDS             complement(<1..813)
                     /locus_tag="PK590_07740"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG24526.1"
                     /translation="METFVLLYLATALAVWIYRSFRSLSELARAPELRVSPQNTRTGS
                     VSILIPAKNEEKNIADCVKSLLTQLRENDEIIVINNCSSDRTEIILKELGALEITENR
                     RPSPQKKTSSTLKYLNTPKTPDGWTGKNFALHLGKNHATGDWFLFTDADTRHQPGGVD
                     ASVAYAEKEALDLLTLFPRCLAHGFWENLIQPCAMGYMGLWFPVRKINDPKSKICFGN
                     GQYLLVRKDHYLGFGGHEALKEAFLEDFAMMKKTKESGARGLCAFGMEVYGTR"
     gene            1001..1717
                     /locus_tag="PK590_07745"
     CDS             1001..1717
                     /locus_tag="PK590_07745"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOG24527.1"
                     /translation="MANLLALNLETLKAAQSLDGEIHQANLLLTQIPEKRSHLSQELE
                     GKKSRLTELDKSLKDLQLNLKEKEMELSQKEAQVKKLDGQLSLVKTNKEYSALQQEIA
                     SLKADNSLLEEDIIKKMDEVEVVKNEVQTEKNHFADIAKSYQTQDQELLAQEKELQKR
                     LQEITKQRQDVVARLPREIGDLYNRIAQKKDGMALASVTGEVCSACQMRIRPQVINEL
                     TLGEQIIVCENCSRILFFDV"
BASE COUNT          467 a          404 c          448 g          406 t
ORIGIN      
        1 ccgcgtccca taaacttcca ttccaaacgc gcagaggccc ctcgcgcctg attcttttgt
       61 ttttttcatc atcgcaaaat cctcgagaaa ggcttctttg agagcctcat gcccgccaaa
      121 accgaggtag tggtctttgc ggacgagaag atattgaccg ttaccaaaac agatttttga
      181 ttttgggtcg ttgattttcc ggacggggaa ccagagcccc atgtagccca tggcgcaggg
      241 ctggataagg ttctcccaga acccatgggc gagacaccgc gggaaaaggg ttaaaagatc
      301 cagggcctct ttttccgcgt aggccaccga agcgtcgacg ccaccgggtt gatggcgagt
      361 atccgcgtct gtgaaaagaa accagtcacc cgtcgcgtgg tttttcccga gatggagcgc
      421 gaagtttttc cccgtccagc cgtcaggagt tttcggggta ttcaagtatt tcaaagtgga
      481 ggaagttttc ttttgagggg aagggcgcct gttctccgta atttcaaggg cgcccaattc
      541 cttgagaatg atctcggtac gatctgacga acaattatta atcacgatga tctcgtcgtt
      601 ctcgcggagt tgtgttaaaa gggacttcac acaatccgcg atgtttttct cttcgttttt
      661 tgctgggatc aggatcgaga cagaacccgt tcgggtgttt tggggtgaaa cccgcagttc
      721 tggcgcgcgg gcaagctccg ataatgagcg gaaactgcgg taaatccata cggccaaggc
      781 ggtggcaaga taaagcagaa cgaaagtttc catagacccg caaatctaaa cgaatggtat
      841 gacccggtca agaaaaagta cggcttggat gctccgtgga atgaaatcct gattgagcga
      901 gtgaaacgaa aatgaccgtg cggatatttc gaaatcgtgg aacagttttt cctgattttt
      961 cttgaaggga aggggtatct cttttataat cgcgccactt atggcaaacc tcctagcgct
     1021 aaatcttgaa actcttaaag cggcacagtc ccttgacggg gagatccatc aggccaacct
     1081 tctcttgaca caaatccccg aaaagcggag ccatctttcc caggaactgg aaggaaaaaa
     1141 gagccgtctc accgagcttg ataaatctct gaaagatcta cagctgaatc tgaaggaaaa
     1201 ggagatggag ctttcccaga aggaagcgca ggtcaaaaaa cttgacggcc agctttctct
     1261 tgtcaaaacg aacaaagaat acagcgctct ccagcaggag atcgcgtcgc ttaaagcgga
     1321 taactcgttg ctggaagaag atatcataaa aaaaatggac gaggtcgagg tggtcaagaa
     1381 cgaagtccaa acagaaaaga accatttcgc ggatattgca aagtcctacc agactcagga
     1441 tcaggaactt ctcgctcagg aaaaagagtt gcaaaagaga cttcaggaaa ttacgaaaca
     1501 gcgacaggac gttgtggcgc ggcttccacg tgaaattggc gatctctaca accggattgc
     1561 tcagaaaaaa gacgggatgg cccttgcttc cgtcaccgga gaagtttgca gcgcgtgcca
     1621 gatgcggatt cgccctcagg taattaatga gcttacgttg ggggagcaga tcatcgtttg
     1681 cgagaattgc tcccgtattc ttttctttga cgtctgatta ctctc
//
LOCUS       DAOSIO010000253         1702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_61237_length_1702_cov_3.582271, whole genome shotgun sequence.
ACCESSION   DAOSIO010000253 DAOSIO010000000
VERSION     DAOSIO010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61237_length_1702_cov_3.582271"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /gene="fusA"
                     /locus_tag="PK590_07750"
     CDS             <1..731
                     /gene="fusA"
                     /locus_tag="PK590_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545225.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOG24528.1"
                     /translation="TLIGGMGELHLDIKVDILRREYGVQVNVGKPQVAYKETILGMAE
                     AEGKFIKQTGGRGQYGHCYLKVESLERGKGIEFENKIVGGAIPREYIPAVEDGVMDAC
                     NNGVLAGYPVTDVKATVFDGSYHEVDSSEIAFKMAGIFAFKEAFKKAKPILLEPIMLV
                     EVLVPEEYMGDVIGDLNSRRCVIQELSNRGHLKSIRGLVPLSEMFGYATAVRSLSQGR
                     ATYTMEPNTYQEVPKMIAEKIIAG"
     gene            926..1237
                     /gene="rpsJ"
                     /locus_tag="PK590_07755"
     CDS             926..1237
                     /gene="rpsJ"
                     /locus_tag="PK590_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015493774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOG24529.1"
                     /translation="MAVKEKIRIKLKAYDHRILDQSVQEIVATAKKTGAKVIGPIPLP
                     THIWKTTVLRGPTIDKKARDQFEIRTHKRLIELEEPTSRTVDALMKLDLPAGVDVEIK
                     L"
     gene            1249..1551
                     /locus_tag="PK590_07760"
     CDS             1249..1551
                     /locus_tag="PK590_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24530.1"
                     /translation="MLGLLGKKIGMSRIFDEGGTQVPVTLIEVGPCFVTGIRTKEKNG
                     YQAVQIGFDVVKERKLTKSRLGATKKIGVPPVNVNREIRTENLEGLDLGSEIRVEF"
     gene            1529..>1702
                     /gene="rplC"
                     /locus_tag="PK590_07765"
     CDS             1529..>1702
                     /gene="rplC"
                     /locus_tag="PK590_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOG24531.1"
                     /translation="MKSGLNFEAGEFVDVVGTSIGKGFQGVVKRLGYKGGASKGHGSM
                     FGRQPGSIGSQSGS"
BASE COUNT          444 a          320 c          498 g          440 t
ORIGIN      
        1 aaacccttat cgggggcatg ggtgaacttc atctggatat caaggtcgat attttaaggc
       61 gcgaatatgg ggttcaggtc aatgtcggga aaccccaggt ggcgtacaaa gagactatct
      121 tggggatggc ggaagcggaa ggaaaattta ttaaacagac cggcggtcga ggtcagtacg
      181 gacattgcta tctcaaggtt gagtcgcttg agcggggcaa gggtattgaa tttgaaaata
      241 agatcgtggg cggcgccatc ccaagggaat atattcccgc tgtggaggat ggcgttatgg
      301 acgcttgcaa caacggtgta ttggcgggtt atcctgtcac ggacgtgaag gcgacggttt
      361 ttgacgggag ttatcacgaa gttgactcct ccgaaattgc ttttaaaatg gccggaatct
      421 tcgctttcaa agaggcgttc aagaaggcga agccaattct ccttgagccg attatgctgg
      481 ttgaggttct tgtgcctgag gaatacatgg gggatgttat cggagatctt aattcgcggc
      541 ggtgtgttat tcaggaactg agtaatcggg gacatttaaa atccattcga ggactggtgc
      601 ctctttctga aatgtttggt tatgcgacgg cggtgcgctc tctctctcag ggacgagcga
      661 cctatacgat ggagccaaat acctatcagg aagtcccgaa aatgatcgcg gagaagatta
      721 tcgcgggctg atggaattgt gttttgagat gcaaagttcc atcgcggcgt gatggtgaac
      781 tgcattcctt agctttttca ctgaataaaa agcgagaggt acactttact taaatttttt
      841 gctctttcaa aaagtgagcg agtaaggtgc ggtggtcaaa gggcgttcat tttatggatg
      901 tctgcttatt aacggagcaa acaaaatggc agttaaagaa aaaatacgta tcaaattaaa
      961 ggcgtacgat catcggatcc tagatcagtc tgtccaggag attgtcgcaa cggctaaaaa
     1021 aacgggcgcg aaagtcattg gcccgatacc gcttccgacc catatctgga aaacaaccgt
     1081 gcttcgggga ccgaccatcg ataaaaaagc tcgtgatcag tttgaaattc gcactcacaa
     1141 aaggcttatt gagcttgaag agcccacgtc ccggacggtt gatgctttga tgaagctgga
     1201 tctgccggcg ggcgttgatg tcgagataaa actttaaagg aaaaagatat gttgggtctt
     1261 ttggggaaaa aaattggaat gtcgcggatt ttcgatgagg gtggcactca ggttccggtg
     1321 actttgattg aagtcggccc ttgtttcgtg accgggattc gcacaaagga aaaaaacggc
     1381 tatcaggcgg ttcagattgg ttttgatgtc gtgaaagagc gcaagcttac caagtcccgt
     1441 cttggcgcga ctaaaaagat tggtgtccct ccggttaacg ttaatcgcga aattcgcacg
     1501 gaaaatcttg aaggtcttga tcttggcagt gaaatccggg ttgaattttg aggctggaga
     1561 gtttgtggat gtggtcggga cttcgatcgg gaagggattc caaggggtcg tgaagcgtct
     1621 tggttataag ggaggcgctt caaaaggcca tggttcgatg tttgggcggc aacctggaag
     1681 tattggttca cagtctggca gt
//
LOCUS       DAOSIO010000254         1697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_61513_length_1697_cov_3.763094, whole genome shotgun sequence.
ACCESSION   DAOSIO010000254 DAOSIO010000000
VERSION     DAOSIO010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61513_length_1697_cov_3.763094"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1486)
                     /locus_tag="PK590_07770"
     CDS             complement(<1..1486)
                     /locus_tag="PK590_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOG24532.1"
                     /translation="MKYILLATIVGWGGGLTNYFLWYDIPIPPYGNILVSAYIVIVAY
                     VIIKYQLMDVRLALTFTSLFLGVYGLVFGIPVLIVLSTQKELELFWGNKWWVIPVLTY
                     AVLSMIGPYIYSKLRLKVENRMLREQRAYQQTLLQASRGMTLVKDMDKLLKLIVHILT
                     KTVKITHARIFLWDPDRKRYESRAVRGASRQGGVSVVDEDSELVRRLREGKQALVLEE
                     LQSQSLASETIIKEMIAHGAALIIPSFIQDRLLGFLVLGEKKSKQLYTESDLDILKTL
                     ANQAALAIENCIFLTEFEHQQAHFFQAAKMADLGTMASGIAHQVNNRFNVIKLGAESA
                     LMVDFPRIEKQMAANNLEGIRPLIGSLESVYRKIAKNADHGGEIVRRLLDFSRLSEGF
                     GPVNIGDIVASCVRLWECKHPVYDTDLRIEIPDGLARIHGNSGEIEEVLFNLLDNAYD
                     AIKMKEEAWECGKLEKPEHPAKGRITLSAREALHMAARAGAAEGL"
BASE COUNT          478 a          463 c          360 g          396 t
ORIGIN      
        1 agaggccctc cgcggcgccg gcgcgcgcgg ccatatgaag cgcctcccgg gcgctcagcg
       61 taatccgccc cttggccgga tgctcgggtt tttcaagttt gccgcactcc caggcctctt
      121 ctttcatctt gatcgcgtcg taggcattat ccagcagatt aaaaaggact tcctcaattt
      181 cgccggaatt tccgtgaatc ctggcaagac catcaggaat ttctatcctg agatcagtgt
      241 cataaaccgg atgcttacat tcccacaggc ggacgcaact cgctacgatg tccccgatgt
      301 tcacagggcc aaacccttcg ctcaaccggc tgaaatcaag aagccttcgg acaatttcac
      361 ccccgtgatc ggcgtttttc gcgatcttcc ggtacaccga ctccagcgac ccaatcagcg
      421 gcctgatccc ttcaaggttg ttcgcggcca tctgcttctc gatccgcgga aaatccacca
      481 tcagcgccga ctccgctccc agcttaatga cattgaaccg gttattcacc tgatgagcga
      541 tccccgaggc cattgtccca agatccgcca tcttcgccgc ctgaaaaaaa tgcgcctgct
      601 gatgttcaaa ttctgtcaga aaaatacagt tctctatcgc gagagcggcc tggttcgcga
      661 gagtctttaa aatatcaaga tcactttccg tgtaaagttg ctttgacttc ttttcgccaa
      721 ggacaagaaa accaagaaga cgatcttgaa taaaacttgg aataataagc gcggcgccat
      781 gggcgatcat ttccttaatg atggtttccg acgcaaggct ttgggactgg agttcctcga
      841 gcacaagcgc ctgtttaccc tcgcggagcc ggcgcaccag ctctgaatcc tcatcaacca
      901 cactcacgcc cccctgacga gaggctccgc gaactgcccg agattcgtaa cgtttcctgt
      961 ccggatccca taaaaaaatt cgggcatgcg tgatcttgac ggtcttggtg agaatatgaa
     1021 caatcagctt gagaagtttg tccatgtcct tcacaagcgt cattcccctc gatgcctgca
     1081 gaagagtctg ctggtatgcc cgttgttctc gcaacatgcg attttcgact ttcaagcgca
     1141 actttgaata aatatagggt ccgatcatcg aaagaactgc gtaggtcagc acaggtataa
     1201 cccaccactt atttccccaa aaaagctcta attctttttg ggttgataga acaattaata
     1261 cagggattcc aaaaaccagg ccgtaaacac ctaaaaataa gctggtaaat gtcagggcaa
     1321 gacgaacatc catcaactga tatttgataa taacgtaagc tactatgaca atataagcag
     1381 aaaccagaat attcccataa ggcggtatcg gaatatcgta ccaaagaaaa taattggtta
     1441 atccccctcc ccaaccaaca atggttgcaa gtaaaatata tttcatttgg tttcgaacaa
     1501 ttccagaagc gcttctaaaa ctctcgaaaa tcagcttaac tccataagcg actacgacaa
     1561 cccaaaacaa caaaaatgga tgaaaaagga atcctggatt tggccaaaac ggataggaca
     1621 atttcggagc tactccggaa acaaaaaatg gcgtgaagct gaataaggac gatgacacag
     1681 caaacacata cgcccct
//
LOCUS       DAOSIO010000255         1690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_61900_length_1690_cov_4.377370, whole genome shotgun sequence.
ACCESSION   DAOSIO010000255 DAOSIO010000000
VERSION     DAOSIO010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61900_length_1690_cov_4.377370"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="PK590_07775"
     CDS             complement(<1..162)
                     /locus_tag="PK590_07775"
                     /inference="COORDINATES: protein motif:HMM:NF014458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOG24533.1"
                     /translation="MLDLKFIRENPEIVKKGISAKGVDFDLDALLALDSRRRNLLKEV
                     EELKAQRNET"
     gene            complement(236..>1690)
                     /locus_tag="PK590_07780"
     CDS             complement(236..>1690)
                     /locus_tag="PK590_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOG24534.1"
                     /translation="LKQMLEKFIEYRAEVIIRRTRFDLDKAEKRAHILEGYKKAIANI
                     DKVIKIIRTSKSPELAKETLMKTLKLSEIQAKAILELQLQRLTQLERLKIDEEYLELI
                     KKIEYYQSVLKSRRKVLDLIKEESQEILKKYGDERRTLIVAAEDDIDIEDLIAQEDVL
                     VSISHAGYIKRIPVSMYRRQHRGGAGVTGAGVRDDEDFIEHMFMASTHDYILFFTNLG
                     RVYWRKAYDIPQGSRQAKGKAIVNFLSLSQNETVSSFLQVKEFSDKQYAIMVTAKGVI
                     KKTNMIEYSRPRSTGVNAITLRDKDELVACKITSGKDEIFMATREGKAIRFKETDVRD
                     MGRTATGVRGMNLAKTDEIVAVEVVDPKMKALTITQNGFGKSTPFVQYRIQSRGGKGI
                     INVKVSEKNGKVVNVLTVADEDEIILVTNNGMVVRCPVNQIRTSGRNAVGVKVMRLHD
                     KDKIASATRVVAKEEGSGKDAQQLPLLDEKNGKK"
BASE COUNT          356 a          451 c          386 g          497 t
ORIGIN      
        1 ggtctcgttc cgctgagcct taagctcttc cacttctttg aggagatttc ggcgccgaga
       61 atccagcgcc agcagggcgt caagatcaaa gtccacccct ttggcagaaa tacctttttt
      121 gacaatctcg ggattttcac ggataaactt gaggtctagc atattggctc gtccatgttt
      181 cgaagattaa actgcgtcct ccccgaaaag gtttttcggg agagagtttt gttttctact
      241 tttttccgtt tttctcgtcc aggaggggca gttgttgcgc gtcttttccc gatccttcct
      301 ctttggcaac cacgcgtgtc gcggaagcga tcttgtcttt gtcgtgcaat cgcattacct
      361 tgactccgac cgcgttacgg ccggaggtcc ggatctggtt gaccggacaa cgaaccacca
      421 taccgttgtt ggtgaccaga atgatttcat cttcgtccgc cacggtgagg acattgacaa
      481 cctttccgtt cttttctgaa actttgacgt taatgatgcc tttgccgccg cgcgactgta
      541 tacgatactg gacaaaaggc gtacttttgc cgaagccatt ttgagtaatg gtcagcgctt
      601 tcattttggg atccacgacc tccacggcga cgatctcgtc ggttttcgcg agattcatgc
      661 ctcgcacacc ggtcgcggtg cgccccatat cacgaacatc cgtttctttg aagcgaatgg
      721 ctttcccttc ccgggtcgcc ataaaaattt catctttccc cgatgtaatt ttgcacgcca
      781 caagctcatc tttgtcgcga agagtgatgg cgttaacgcc cgtggagcgc ggccttgaat
      841 attcgatcat gttcgtcttt ttgatcactc ccttggccgt caccatgatc gcatactgct
      901 tatcgctaaa ctccttgacc tggagaaaac ttgaaacggt ctcgttttga ctcagcgaaa
      961 gaaaattaac gatggccttc cctttggcct gtctggagcc ctgggggata tcataggctt
     1021 ttcgccagta cactcgcccc aggttcgtga aaaaaaggat atagtcgtgg gtcgaggcca
     1081 tgaacatgtg ctcgataaaa tcctcgtcat cgcgcacacc cgctcccgtg acgcccgctc
     1141 cgccgcggtg ttgccggcgg tacattgaca cggggatccg cttgatatag cccgcgtgcg
     1201 aaatactaac aagcacatct tcctgcgcga tcagatcctc aatgtcgata tcatcttccg
     1261 ctgcaacgat taaagtcctt cgctcatccc cgtatttttt aaggatttcc tgcgactctt
     1321 ccttgatcaa gtcgagcact ttccggcgac ttttcaatac cgactggtaa tattcgattt
     1381 ttttgatgag ttcaagatat tcttcgtcaa tttttaatct ctcaagctgg gtcagccgct
     1441 gaagttgaag ttcaagaatg gccttggcct gaatttccga aagcttcagc gttttcatca
     1501 aagtttcttt ggcaagctcc ggactttttg acgtccgaat aatcttgatg accttatcaa
     1561 tgttcgcgat cgctttttta tatccctcaa gaatatgggc gcgcttttcc gctttatcga
     1621 ggtcaaatcg ggtccggcga atgataacct cggcccgata ttcgataaac ttttcgagca
     1681 tctgcttgag
//
LOCUS       DAOSIO010000256         1689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_61961_length_1689_cov_3.435741, whole genome shotgun sequence.
ACCESSION   DAOSIO010000256 DAOSIO010000000
VERSION     DAOSIO010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61961_length_1689_cov_3.435741"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PK590_07785"
     CDS             <1..492
                     /locus_tag="PK590_07785"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOG24535.1"
                     /translation="EHNGGIGYLISTSKKLTEIMRSRIDNGIIIQVKSLAEACEVANE
                     IAAEHLEIMTEDPEKVQKSIRHAGAIFVGPYSPAVIGDYYAGPSHVLPTGGTARYFSP
                     LSASTFIKASQTIRYTPEALEASKEHVQKLTEMEGLYLHRISLEARLARNTPEPEKEK
                     NPL"
     gene            489..1085
                     /gene="hisB"
                     /locus_tag="PK590_07790"
     CDS             489..1085
                     /gene="hisB"
                     /locus_tag="PK590_07790"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOG24536.1"
                     /translation="MKKRSAIIKRKTSETDIAVVLTIDGKGQSSVQTGIPFLDHMLTL
                     LAKHGLLDLKLKAKGDLEVDIHHTNEDTGIALGQALAKALGNKKGIRRYGSFAVPMDE
                     ALIRCTLDISSRPSLYLKKFPGVRLTRQAKYTLDDAADFLRSFCQHAGINMHIGVLAG
                     EDTHHIVEGIFKALAKALDMAMTRDPRVKGIPSTKGKF"
     gene            1116..>1689
                     /gene="hisH"
                     /locus_tag="PK590_07795"
     CDS             1116..>1689
                     /gene="hisH"
                     /locus_tag="PK590_07795"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOG24537.1"
                     /translation="MITIIDYGMGNLRSVSKALTHVGARCRFVVTGSQLKGSKKIILP
                     GVGAFGDAMRELKNRNFVEPLANSIAKGAKLLGVCLGLQLLFETSEEAPGIKGLGFLQ
                     GKVRRFRSAREKIPHMGWNNIRITGKHPLLKGIRSGDYFYYVHSFYGEPQLKKITKAT
                     SRYAGRDFAAVIGNGQIAATQFHPEKSQTTG"
BASE COUNT          446 a          426 c          424 g          393 t
ORIGIN      
        1 gagcataatg ggggaatcgg ttatctcatt tccacttcca agaaactcac ggaaattatg
       61 cgttcccgca tcgacaacgg catcattatt caggtcaaat cgctggccga ggcctgtgag
      121 gtggccaatg aaattgcggc ggagcacctt gaaatcatga ctgaagatcc ggaaaaagtc
      181 caaaaatcca tccgtcacgc gggcgctatt tttgtcggcc catactctcc ggctgtgatc
      241 ggcgactatt acgcgggtcc cagtcacgta ttgcctaccg gaggcaccgc gcgttatttt
      301 tccccgctta gcgcgtcaac ttttatcaag gcttcacaga cgatccggta tacaccggaa
      361 gcgctggaag cctctaaaga acatgtgcaa aaacttacgg aaatggaagg attatacctg
      421 caccgaattt cccttgaggc gcgtctggcc agaaacacgc cagaaccgga aaaggagaaa
      481 aatcccctat gaaaaaacgt tcagcgatca ttaaacgaaa aacttcagaa accgatatcg
      541 cggtcgtcct gacaattgat ggcaaaggtc agtcttccgt ccagactgga atcccttttc
      601 tcgatcacat gttaacgctc cttgccaagc acgggctact ggatctcaaa ctcaaggcga
      661 agggggatct cgaagttgat attcatcata ccaatgagga taccggaatt gccctcgggc
      721 aagccctcgc gaaagcgctc ggcaataaaa agggaatccg tcgttacggg tcgtttgccg
      781 tcccgatgga cgaagcttta atccgttgca cactggatat ttcgagtcga ccctcgcttt
      841 acctcaaaaa attccccggg gtgcgtttga cccgccaagc gaaatatacc ctggatgacg
      901 cggctgattt tctccgctcg ttttgtcagc acgctggcat caatatgcac atcggagtgc
      961 ttgccgggga agacacgcat cacattgtcg aaggtatctt taaggctctt gcgaaagctt
     1021 tggatatggc tatgacacgg gatcctcggg tcaagggtat tccctcgacc aagggaaagt
     1081 tttaacgccc ccaatttaaa atctcgagat ttttgatgat cacgattatt gactatggaa
     1141 tgggaaattt acgcagcgtt tcgaaagcgc tgactcatgt tggcgctcga tgccgttttg
     1201 tcgtcaccgg ctcccaatta aaaggttcga aaaaaatcat cctgccgggc gtgggtgcct
     1261 tcggagacgc gatgcgcgag ctgaagaacc ggaactttgt cgaaccgctc gcgaattcaa
     1321 tcgcaaaagg cgccaagctt ttgggagtat gtctgggact gcaactttta tttgaaacaa
     1381 gcgaagaggc tccgggcatc aaggggctgg gttttttgca gggcaaggtc cggcgatttc
     1441 gctcggctcg tgaaaagatc ccccacatgg gctggaacaa tattcgaatc accggtaaac
     1501 atccccttct taaggggatt cgaagtggag attatttcta ttacgttcat tcattttatg
     1561 gcgagccaca actaaaaaaa atcacaaagg caacttcccg ttacgcgggc agggattttg
     1621 cggccgtgat cggaaatggc caaatcgcgg caacgcaatt tcaccccgaa aaaagtcaga
     1681 cgacggggc
//
LOCUS       DAOSIO010000257         1681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_62378_length_1681_cov_4.490775, whole genome shotgun sequence.
ACCESSION   DAOSIO010000257 DAOSIO010000000
VERSION     DAOSIO010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62378_length_1681_cov_4.490775"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PK590_07800"
     CDS             <1..397
                     /locus_tag="PK590_07800"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HOG24538.1"
                     /translation="SDQTGRPTYTKDVAGAILRMLSKKKEMVFDSGVRIYHISNSGQV
                     TWAGFARFILEKLGRSDVTVEEISSRELNRPAPRPYNSVLSLKKTESALGIQFRPWED
                     AVADFLVELADTKKKEAAKAQEKAEGDPQ"
     gene            405..1361
                     /locus_tag="PK590_07805"
     CDS             405..1361
                     /locus_tag="PK590_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG24539.1"
                     /translation="MAEQKTVLVTGGGGFLGSHLCDRFVARGFRVYAMDNFLTGSEQN
                     IAHLDSHKDFELIRHNVCEYTNFPGHLDYILHFASPASPLDYLQYPIQTLKVGALGTH
                     NILGLAKEKKAVFLLASTSEVYGDPQVHPQKEEYWGNVNPVGPRGVYDEAKRYAEALT
                     MAYHRSHRIDTKIIRIFNTYGPRMRLKDGRAIPAFITQALTGQPLTVFGRGKQTRSLC
                     YITDMIDGIEKLLMSSLNEPINIGNPREISMMELAKLILRITGASSGIVHQDLPVDDP
                     KVRRPDITKAKKNLNWQPKVSLESGLQKTVDYFKTMITNRES"
     gene            1363..>1681
                     /locus_tag="PK590_07810"
     CDS             1363..>1681
                     /locus_tag="PK590_07810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG24540.1"
                     /translation="MAPYLSIIIPTYNEEKRLPGTLDFIFAFLRKQTYEAEIIVSDDG
                     SRDRTVKISAEKLRFSDSQVLVSPRNVGKGDAVRRGMLAAKGQYLLITDADLSTPIEE
                     VRRF"
BASE COUNT          434 a          404 c          449 g          394 t
ORIGIN      
        1 gtcggaccag acgggacgac cgacttacac gaaggacgtg gccggcgcta ttcttcggat
       61 gttgagtaaa aaaaaagaaa tggtatttga ctccggagtc cggatatatc atatctcaaa
      121 ttccggtcag gtcacttggg ccggattcgc gcgttttatt ttggaaaaac ttggtcgtag
      181 cgatgtgacg gtcgaggaaa tctcttcacg cgaactgaac cgtcccgcgc cacgaccgta
      241 taattcggtt ttatctctga aaaaaacgga atcggcgctc ggaatccagt ttcgtccctg
      301 ggaggacgcg gttgcggatt ttctggtcga actggcggat acgaaaaaga aagaagcggc
      361 aaaggcgcaa gaaaaggccg agggtgatcc ccaatagagg atttatggca gaacaaaaga
      421 cagtgttggt aaccggtggc ggtgggtttc tagggtcgca tctttgtgac cgttttgtgg
      481 cgcggggatt tagggtgtac gcgatggaca atttccttac cggaagtgag cagaatatcg
      541 ctcatctgga ttcccataaa gattttgaac tgatccggca caatgtttgt gagtacacga
      601 atttccccgg tcatctggat tacattcttc actttgcctc gccggccagc ccgcttgact
      661 atttacaata ccccattcaa acgctcaagg tcggcgctct tgggacgcac aatattctcg
      721 gcctcgcgaa ggaaaaaaaa gccgtatttc tactcgcgtc gacttcggag gtttatggcg
      781 atccccaggt gcatccgcaa aaagaggaat actgggggaa cgtcaatccg gtgggacctc
      841 ggggtgtcta cgatgaagcc aaacgctacg cggaggcgct cacgatggcg tatcaccgtt
      901 cccatcgaat cgatacgaaa attatccgga tttttaacac ctacggacca cggatgcgcc
      961 tcaaagacgg tcgcgcgatt ccggcgttta taacgcaggc cctgacagga caacccttga
     1021 ccgtgttcgg gcgggggaag cagacacgca gtctttgtta tattactgac atgattgacg
     1081 gaattgaaaa actgctaatg tcttccctga acgagccgat caatattgga aatccccggg
     1141 aaatttccat gatggaactg gcgaaactca tcctccggat tacgggggcc tcgtccggaa
     1201 ttgtccatca ggatttgcca gttgacgatc cgaaggttcg ccggccggat attacgaagg
     1261 cgaaaaaaaa ccttaactgg caaccgaagg tgtccttgga atcgggactt caaaaaactg
     1321 ttgattattt caaaaccatg attacaaacc gcgagagcta atatggcccc ttatttatct
     1381 atcatcatcc cgacttataa cgaagagaaa cgtcttccgg ggacgctgga ttttattttc
     1441 gcgttcctcc gcaagcaaac ctacgaggct gaaattatcg tttccgatga tggcagtcgt
     1501 gaccggacag tcaaaatttc ggccgaaaaa ctgcgctttt cagacagcca ggttcttgtc
     1561 agcccccgaa atgtcggcaa aggggacgcg gtgaggcggg gaatgctcgc ggcgaaagga
     1621 cagtatctgt tgatcacgga cgccgatctt tccaccccga ttgaggaagt gcggagattt
     1681 t
//
LOCUS       DAOSIO010000258         1657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_63687_length_1657_cov_3.713483, whole genome shotgun sequence.
ACCESSION   DAOSIO010000258 DAOSIO010000000
VERSION     DAOSIO010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63687_length_1657_cov_3.713483"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..>1657)
                     /locus_tag="PK590_07815"
     CDS             complement(2..>1657)
                     /locus_tag="PK590_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG24541.1"
                     /translation="LNRYRGYVPCAVCHASRLKPDAMNYRLGGRTIADVQALPIDRLL
                     EFLETIHLSPEEKEKMGPVHEELIRRVRFLKEVGLGYLTLDRMSRTLSGGEVQRIHLA
                     SSLGSALVDTLYVLDEPSIGLHERDNQMLIALLKKLRDLGNTVVVVEHDRTMIQSADE
                     VIDLGPEGGAKGGRLVFQGSIDALKSCQASYTSRYLSGKEKLQRVFRPARGKPRKILI
                     EGASAHNLKKIRAEIPLGRLAVLTGVSGSGKSTLLYNVLYNHYLKSKGQPVSRDELGN
                     VKRIAGFDGVEDMVLVDQSSLGRSPRSNPATYTKAFEEVRKVFASTRQAKRAGFDAGY
                     FSFNSEKGRCPVCKGDGAVKVEMHFLADIFVPCEACQGRRFKNEVLEIEYQGLNIDKV
                     LQLTIDESVDFFQARKAIVDKLEPLRKIGLGYLKLGQPTLTLSGGEAQRLKLACEMAE
                     PFSGAALYLLDEPTTGLHERDIHHLLTAFDELLARGHSLFVIEHHMGLIALADYVIDL
                     GPEGGEEGGEIIYQGDMAGLLECNRSHTGRCLKKYLAGLSAAD"
BASE COUNT          408 a          496 c          378 g          375 t
ORIGIN      
        1 tttaatctgc cgcactcaag cccgcaagat atttcttgag acaccgtccg gtatgagacc
       61 ggttgcattc caacaaaccc gccatatccc cttgataaat aatttcgcct ccctcctcgc
      121 ctccctccgg cccgagatca atcacataat ccgccagagc aatcaatccc atatggtgct
      181 ctatcacaaa aagcgagtgt ccccgggcga gaagctcatc aaaagcggtc aacagatgat
      241 gaatatccct ctcatgcaac cctgtcgtcg gctcatccag aaggtacagc gcggcccccg
      301 agaacggttc ggccatttca caggccagct taagacgctg agcttcacca ccggagaggg
      361 tcaaagtcgg ctgaccgagt ttaagataac caagcccgat ctttcttaaa ggctccagtt
      421 tatcgacaat agctttgcgt gcctgaaaaa aatccacgct ttcatcaatc gtaagttgaa
      481 gaaccttgtc aatattcaat ccttggtatt caatttccag aacttcgttt ttaaaccgcc
      541 ggccttggca cgcttcgcac ggcacaaaga tgtccgcgag gaaatgcatt tcgaccttga
      601 ccgcgccatc gcccttacaa accggacaac gtcctttttc cgaattaaag gaaaaatagc
      661 cggcgtcaaa acctgcccgc ttggcctgcc gggtcgaagc aaaaaccttc cgcacctctt
      721 caaacgcctt ggtgtaagtc gcgggattgg aacgcggtga ccgccccaga gaggactgat
      781 cgacgagcac catatcctcg acgccgtcaa accccgcgat cctttttaca tttccaagct
      841 cgtcccgaga taccggctgg cccttggatt tcaggtaatg gttataaagg acattgtaaa
      901 gcaaggtgct tttcccggat cccgaaactc ccgtcaggac cgccagtcgc cccaatggta
      961 tttcagcacg aatttttttg aggttgtgcg ctgacgcacc ctcgatcagg attttccggg
     1021 gttttccccg cgcaggacgg aacactcgtt gcagtttttc tttccctgaa aggtagcgtg
     1081 acgtataaga cgcctggcac gacttgagag cgtcaatgct tccctgaaaa accaaccggc
     1141 ctcccttggc gcccccttcg ggtccaagat caatcacttc atcagcggac tggatcatgg
     1201 ttcgatcgtg ctcaaccacc accaccgtat tcccgagatc acgaagcttc ttgagaagcg
     1261 caatcagcat ttgattgtcc cgctcatgaa gaccgatgct cggttcatcc agaacataaa
     1321 gggtgtccac aagggccgac cccagcgaac tcgcgaggtg aattcgctgg acttccccac
     1381 cggaaagcgt tcgtgacatg cggtcgaggg tcagatagcc caaaccaacc tcttttagga
     1441 aacggacgcg gcggatcaat tcctcatgga ctggtcccat tttctctttt tcctccgggg
     1501 acaagtgaat cgtttcaaga aattcaagca accggtcaat cggaagggcc tgaacgtccg
     1561 cgatcgtcct tcctcccagc cggtaattca tcgcgtccgg tttaagtcgg ctcgcgtgac
     1621 agaccgcaca gggaacataa ccccgataac ggtttaa
//
LOCUS       DAOSIO010000259         1657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_63689_length_1657_cov_3.426966, whole genome shotgun sequence.
ACCESSION   DAOSIO010000259 DAOSIO010000000
VERSION     DAOSIO010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63689_length_1657_cov_3.426966"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PK590_07820"
     CDS             <1..541
                     /locus_tag="PK590_07820"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HOG24542.1"
                     /translation="IEKEKYLFYGAIGETKVLDELKKLPETYHIINDFGLEFNPPLYN
                     RSEDDRIYSIQVDHLVVGPAGIFITETKYWSNKSISNDMLFSPVKQVKRAGFSLFVLL
                     NDAIKSRHIPRLVNNWGERKISPRQIVASVNSVPHAEFQYVKVLGIEKLNSYITYGKT
                     EFTTEEVQDIVGFLLQYQN"
     gene            complement(865..>1657)
                     /locus_tag="PK590_07825"
     CDS             complement(865..>1657)
                     /locus_tag="PK590_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006303092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOG24543.1"
                     /translation="LKTLPLSLRLICELHKRLLTGVRGGHATPGEFRRAQNWIGQAGC
                     TLANASYVPPPPSELMTCLGAFEKFMHDRSLPPLIQIALLHYQFESIHPFLDGNGRVG
                     RLLITLFLVERKILPTPLLYLSAFFEATRRDYYDLLSGVSERGEWEPWLAYFLNSVAR
                     QSEDALSRAERINQKLGLWRKDMVGVSSKAPLFLIDMLAGNPYLTVNKAAKQLNVAFT
                     TAQRAIDKLEKLSIVTKVGSAKRDRVYCAKVILSILEEPARLVPK"
BASE COUNT          461 a          347 c          404 g          445 t
ORIGIN      
        1 tatcgagaag gaaaaatatc ttttttatgg agcgataggc gaaacaaaag ttttagacga
       61 gttaaaaaaa ctcccggaaa catatcacat catcaacgat tttgggcttg agtttaatcc
      121 accgctttat aaccggtctg aagatgatcg aatatattcg attcaggtag accaccttgt
      181 cgttggtcct gccggcatat ttattacaga aacaaaatat tggagcaata aatcaatttc
      241 aaatgacatg ttattttcac ctgttaagca ggtcaaacgt gctggttttt cgcttttcgt
      301 gcttcttaac gatgctatta agtccaggca tattccgcgg ttggtaaata actggggaga
      361 aagaaaaatc tcgccaaggc aaattgttgc ctccgttaac agcgtccctc atgccgagtt
      421 tcagtatgtt aaagtcctcg ggattgaaaa attgaattca tacatcacat acggaaaaac
      481 agaatttacc accgaggaag ttcaggatat cgttggtttt ctacttcaat accagaatta
      541 agttatatca atgcggatta gcttcgcccc tcggggcccg cgccattttt tgagcgatac
      601 aagtaacgtc acggattgac gataggttat gtgtttggcg tccatctcgg ggagccatta
      661 taagaaaaaa agcgtagggt ctttagatcc tgagcttttt ttatatagac ggttttccac
      721 cgccagtcct gaattcctta caggttctat agaatcaggg caggcggggg agtcttctag
      781 gcaaaagcga gaacctcaga aaatggggtt ctcgtttttt gctgttggga gaaaaaatgt
      841 gaccgatgag tttcaagatc gggtttattt tggaacgagc cgggcaggtt cttcgaggat
      901 cgagagaata accttggcgc aataaacgcg gtcgcgtttt gcggagccta cttttgtaac
      961 aatagacaat ttttcaagtt tgtcgatggc gcgttgtgcc gttgtaaaag ccacattgag
     1021 ttgctttgcg gctttattga cggtcaaata gggattaccc gccaacatat cgattaagaa
     1081 aagcggcgct ttggatgaca cccccaccat gtctttgcgc catagcccaa gtttttgatt
     1141 aatcctttcc gcgcgactga gggcgtcttc cgattgcctg gcaacactgt taagaaaata
     1201 tgcgagccaa ggttcccact ctcctctttc gctaacacct gacaaaagat cataataatc
     1261 acgccgggtt gcttcgaaga aagcacttaa atagagcagt ggtgtcggaa gaattttgcg
     1321 ttcgacgaga aagagagtga tcagtaaacg accgacccgg ccattaccgt ccaagaaagg
     1381 atggatggat tcaaattgat aatgaagaag cgcgatctgg ataagcggcg gaagggaacg
     1441 gtcatgcata aatttctcaa atgccccgag gcaggtcata agttccgaag gcgggggtgg
     1501 gacataactg gcgttcgcaa gcgtgcatcc cgcttgtccg atccaatttt gcgcgcgtct
     1561 gaattctccg ggtgtcgcgt ggccaccccg gactcctgtt agcaagcgct tgtgtaattc
     1621 acagatcaaa cggagagaaa ggggaagggt tttcaat
//
LOCUS       DAOSIO010000260         1640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_64574_length_1640_cov_5.116719, whole genome shotgun sequence.
ACCESSION   DAOSIO010000260 DAOSIO010000000
VERSION     DAOSIO010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64574_length_1640_cov_5.116719"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="PK590_07830"
     CDS             <1..137
                     /locus_tag="PK590_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG24544.1"
                     /translation="QAGVVTPAGTVMLSVMLIVIGLQFVMAFLAYDIASVPNRPLTSS
                     "
     gene            155..1615
                     /locus_tag="PK590_07835"
     CDS             155..1615
                     /locus_tag="PK590_07835"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG24545.1"
                     /translation="MKVFFLNIPFLHRFTRESRSPGVAPSGTLYYPIYLALAAGLVRQ
                     RGYTIDFLDAPAEGYDLKQTEDRVAKFGPDLIVATTVTASVAHDIRVMDHLAKQTGAM
                     TMLVGTHPTALPIETLKASPSLDFVAVGEYDFTVLELVRHFDSGKTRQQLHEVAGLAY
                     RSGEEFILNERRPWHENLDEIPWASLIYRDFLKIEHYSYGAQLHPEVTIVSGRGCPYQ
                     CTFCVFPQTITGQRYRPRSVRDVVDEMEFIQKTWPQNKEIMFEDDTLTVDKTRMRELS
                     EEILRRGLKVTWSGNARADFDDVELLKLMKKAGCRLFCVGFESSDQKILDEMRKGGAT
                     IGIRERFVRACAEAGIMIHGCFLFGGVRETRETMRATLEMAKRMKLDTAQFFPIMVYP
                     GTRAYEEAKREGELVTENYETWLRGSGYYVSQVCRGQLSAEEISAFTDRAYREFYLRP
                     AFIWQKIRQFFVSPSERSRIILGFFFFLKKVFTRDK"
BASE COUNT          348 a          396 c          486 g          410 t
ORIGIN      
        1 cgcaggccgg agtggtgact cccgccggca ccgtgatgct ctcggtgatg ctgatcgtga
       61 ttgggcttca gtttgtcatg gcgtttcttg cttacgatat cgcttccgtt cctaacagac
      121 cattgacgtc ttcttgaact gacaaggtta ttccgtgaaa gttttctttc tgaacatccc
      181 gtttcttcat cgctttacgc gcgagagccg ttccccgggc gtggctccgt cggggacgct
      241 ttattatccc atttatcttg ccctagcggc gggccttgtc cgtcagcgcg gatatacgat
      301 cgactttctt gacgcgccgg ccgaaggcta cgatctcaaa cagactgagg atcgtgtcgc
      361 gaaatttggt ccggacctta ttgtggcgac tacggtgacg gcttccgtgg cccatgatat
      421 tcgagtcatg gatcacctcg cgaaacagac tggcgccatg accatgcttg tcggaaccca
      481 tccgacggcg ttaccgatag aaacgttaaa agcttccccg agccttgatt ttgtggcggt
      541 gggggaatat gactttacgg tactggagct cgtgcggcat tttgactcgg ggaaaacgcg
      601 ccagcaactg catgaagtcg cgggactggc gtaccgttcc ggagaagagt ttattttgaa
      661 cgaaagacgg ccctggcatg agaatctgga cgaaattccg tgggccagtc tgatttacag
      721 ggattttctg aagatcgaac attacagcta cggggcccaa cttcatcccg aggtgacgat
      781 cgtcagcggt cgcggatgcc cctaccaatg tactttttgc gtatttcccc aaacaattac
      841 gggacagcgt tatcggcccc gctcggtcag ggacgtggtg gatgaaatgg agtttattca
      901 gaaaacttgg ccgcagaaca aagagattat gtttgaggac gacaccctga cggttgacaa
      961 gacccggatg cgggaacttt ccgaagaaat tttgaggcgg ggactgaagg tgacatggtc
     1021 ggggaacgcg cgggccgatt tcgatgacgt ggaattgctc aagcttatga aaaaggccgg
     1081 gtgccggctt ttttgcgtcg ggttcgaatc ttcagaccag aaaattttgg atgaaatgcg
     1141 aaagggtggg gccacgatcg ggatccggga gcggtttgtc cgcgcctgcg ccgaggcggg
     1201 gatcatgatc cacgggtgtt tcctttttgg gggtgttcgg gaaacccggg agacgatgcg
     1261 ggcgactctg gaaatggcca agcgaatgaa actggatacg gcccagtttt ttcccattat
     1321 ggtttatccg ggaacccggg cttatgaaga agccaaacgg gaaggggagc ttgtgacgga
     1381 gaattatgaa acgtggctcc ggggaagcgg ctattacgtt tcccaggttt gtcgcggtca
     1441 actgagcgcg gaggaaattt ccgcgtttac ggaccgggct taccgggaat tttatcttcg
     1501 cccggcgttt atctggcaga agatcagaca gtttttcgtc tcgccttcgg agcgatcccg
     1561 catcattctt ggatttttct tttttctgaa aaaagtgttc acgcgagaca agtgatcatg
     1621 cgagttgccg tcgtctccgc
//
LOCUS       DAOSIO010000261         1605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_66561_length_1605_cov_4.481935, whole genome shotgun sequence.
ACCESSION   DAOSIO010000261 DAOSIO010000000
VERSION     DAOSIO010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66561_length_1605_cov_4.481935"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PK590_07840"
     CDS             complement(<1..858)
                     /locus_tag="PK590_07840"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOG24546.1"
                     /translation="MRDNIRVSVLGDGGWGTALALVNTRRGNKVCLWSVSPAYARYLD
                     KRRENSKFLPGIKLPKTLHITSNLDEAVAMADVIVLAIPTRHLRSVLIRLKKLPYQGK
                     IFVSATKGIENKTCLRPSEVVYHVLGRSVRLVVLSGPSHAEEVARKIPTLMVAASKDS
                     KAAEFVQEYFRDPQFRVYVQSDVVGVELGGALKNVIAIAAGVCDGLKFGDNTKAGLMT
                     RGIYEMVRLGVKLGANPGTFFGLSGIGDLITTCSSRHGRNLYVGRELGKGRTMKAILG
                     KMAMVAEGVG"
     gene            complement(942..1364)
                     /locus_tag="PK590_07845"
     CDS             complement(942..1364)
                     /locus_tag="PK590_07845"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOG24547.1"
                     /translation="MEYFDYHYRVIYADTDRMGVTYYGHYFRFFEAGRNEYFRALGLP
                     YTECEKKGVFLPVGEAHAKYIVPSTYDDELIVRTSVSRLGASTINFQYQVIKKDTGKV
                     ITTGDTLHVFVDKTLRPCRIPDEIRSKVTVHTLLRKKT"
     gene            complement(1405..>1605)
                     /locus_tag="PK590_07850"
     CDS             complement(1405..>1605)
                     /locus_tag="PK590_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOG24548.1"
                     /translation="SGTPSGKILKIKNEGVPAFGVPDRRGDLYVRAQVEVPVKPSRQE
                     RELIEKLAAERGDPVAVKKGFF"
BASE COUNT          392 a          481 c          357 g          375 t
ORIGIN      
        1 ccccacgcct tcggccacca tcgccatttt acccaaaatc gccttcatcg ttcgcccttt
       61 tcccagttcg cgtccgacat aaaggttccg gccatgacgg gatgaacacg tggtgataag
      121 atccccgatc ccggaaagtc caaaaaaggt tcccggattc gcgcccaact tcactccgag
      181 ccggaccatt tcataaattc cgcgggtcat aagaccggcc ttggtgttat ccccgaattt
      241 aaggccgtca caaacccccg ccgcgatcgc gatcacattc ttcagggccc ctccaagctc
      301 gacgccaaca acgtcgcttt ggacataaac acgaaactga ggatctcgga aatactcctg
      361 gacaaactcc gccgctttgg aatctttgga ggccgccacc atcaacgtcg gaatttttcg
      421 ggcgacctct tcggcgtgtg atggcccgga aaggacaacc agtcgaacgc ttcgccccag
      481 gacatgatat acaacttcag aaggacggag acatgtttta ttttcgatcc ctttcgtggc
      541 gctgacaaaa attttcccct ggtacggcaa ctttttaagg cgaatgagga cactccgaag
      601 atgccgggtc ggtatcgcta aaacaataac gtcggccatc gcgaccgctt cgtcaagatt
      661 cgacgtgatg tgaagcgttt tgggaagttt gataccgggg agaaacttgc tgttttcccg
      721 tcttttatca agatacctgg cgtacgcggg ggaaacggac caaaggcaaa ccttattccc
      781 gcgcctcgtg ttcacgagcg caagcgccgt tccccagcca ccatccccaa gcactgagac
      841 cctgatgttg tccctcatgt cattccccct tttcatggcg cacactcaac gtcaaaattt
      901 cagcagagat acgaaccatc ggcctgtccg aagccacgca ttcacgtctt tttacgcaac
      961 aaagtgtgca ccgtcacctt gctcctgatt tcatccggaa ttcggcaggg acgcaaagtc
     1021 ttatccacaa aaacgtgcaa tgtgtctccg gtcgtaatca ccttccccgt atctttttta
     1081 atcacctgat actggaagtt aatcgtgctg gcacccaaac ggcttacgct ggtgcggacg
     1141 atcaattcgt catcatacgt ggagggtaca atgtacttgg catgcgcctc gcccacagga
     1201 agaaagacgc cttttttttc gcattcggta tacggcaaac caagcgcgcg aaaatactca
     1261 ttacgccccg cttcaaaaaa ccggaagtag tgaccgtaat aagtcacgcc catccgatcg
     1321 gtatccgcat aaatcactct ataatgatag tcaaaatact ccacagttcc accttagggt
     1381 cgcccccgaa atgtgaggtt atgattagaa aaaacccttt ttaacggcca ccggatctcc
     1441 tctttcagcc gcgagctttt ctattaactc ccgttcctgc ctggacggct taaccggaac
     1501 ctcgacctgg gcacggacgt aaagatctcc gcgccgatcc ggaaccccga acgcgggaac
     1561 tccctcgttt ttgattttga gaatttttcc ggacggagtc cccga
//
LOCUS       DAOSIO010000262         1593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_67328_length_1593_cov_3.255527, whole genome shotgun sequence.
ACCESSION   DAOSIO010000262 DAOSIO010000000
VERSION     DAOSIO010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67328_length_1593_cov_3.255527"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>451)
                     /locus_tag="PK590_07855"
     CDS             complement(<1..>451)
                     /locus_tag="PK590_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG24549.1"
                     /translation="LMARSAAMEAEQMDLSKEAGSFFREAVFDHGTFFTPKMGFAGRE
                     FFKNPETGEVYLETEYDVFPTGSFVGVYLKTRDFDASRHEGLTFEIRRRGEEGVPDNF
                     FIEFKSKGNVIRKFSPHGFLREWTPFEFKFSATKPTPINEVVFVFTND"
     assembly_gap    452..461
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<462..1295)
                     /locus_tag="PK590_07860"
     CDS             complement(<462..1295)
                     /locus_tag="PK590_07860"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOG24550.1"
                     /translation="MPTELAALRAPSVPFKKGSDFVKQTHGILINAHQICLRGTGDFQ
                     NTRAQSPEGEDNYMKIHVEKPQKSRLILPVIIVLIVIGLVFLLPHLSPKDSLPSLAYE
                     VTGFEGDVEITDALDQEWRAPKRGEEFVSGQKIRTGADGIINLQVEDEIRLRLKENAL
                     LANRECKSQGGKEIYHLGLDQGVLLGATTKPFDKKVSEDKANFMVVTPVFNAIPRGAL
                     FRVSASPQETGNKVGVLRGVVEIFKQFQLFSQGGYKIRGLEESVFLNGEVQPPTRLTP
                     EA"
     gene            complement(1316..1393)
                     /locus_tag="PK590_07865"
     tRNA            complement(1316..1393)
                     /locus_tag="PK590_07865"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1356..1358),aa:Val,seq:cac)
BASE COUNT          421 a          433 c          357 g          372 t
ORIGIN      
        1 gatcattggt aaacacgaaa acaacctcgt taatcggcgt gggcttcgtc gcgctaaact
       61 tgaattcgaa cggggtccac tccctcagga aaccatgcgg cgaaaacttg cgaatcacgt
      121 ttccctttga tttgaattcg atgaaaaaat tgtcgggaac cccctcctcc ccgcgtcgac
      181 gaatttcaaa agtcagcccc tcgtggcggg aagcgtcaaa atcacgggtt ttgagataaa
      241 ccccgacaaa cgaaccggtc ggaaaaacgt catattcggt ctcaagatag acctctccgg
      301 tctcggggtt cttgaaaaat tccctgccgg caaaacccat tttcggcgta aaaaacgtac
      361 cgtgatcaaa cacggcttcc cgaaaaaaac ttccggcttc tttggaaaga tccatctgct
      421 cggcttccat ggccgccgac cgggccatca gnnnnnnnnn ncgcttcggg ggtcagcctg
      481 gtgggaggct ggacttcacc gtttaaaaaa acggattctt caagaccgcg gattttatat
      541 cccccctgag aaaacagttg aaactgcttg aagatctcga ccacaccgcg caagaccccg
      601 accttgttcc cggtctcctg gggggacgct gagacacgaa acagcgcgcc tctgggaatc
      661 gcattgaaca ctggtgtgac aaccataaaa ttggccttat cctcagaaac tttcttgtca
      721 aagggcttgg tggtcgcccc aagcaaaacc ccttgatcca gaccaaggtg gtaaatttct
      781 ttgccacctt gggacttaca ttcgcgattc gcgagaaggg cattctcctt aagacgcaac
      841 cggatttcat cctccacctg aagattgata attccgtcag ctccggtcct gattttctgt
      901 ccggatacaa actcttcccc ccgtttgggc gcgcgccatt cctgatccaa tgcgtctgta
      961 atttccacat caccttcaaa gccggtcacc tcataagcca gggaaggtaa tgaatctttt
     1021 ggggaaagat ggggtaaaag gaaaaccaac ccaataacaa ttaaaacgat gatcacagga
     1081 agtatcaaac gagatttttg aggtttctcg acatggattt tcatgtaatt atcctctcct
     1141 tccgggcttt gcgcccttgt attctgaaaa tcgcctgtgc cccgcagaca aatttgatgg
     1201 gcattaatta aaataccgtg tgtttgtttg acgaaatccg aacctttttt gaacggaact
     1261 gacggcgcgc gaagcgcggc caattcggtc ggcaaaaatc ctgaaagttt aatactggtg
     1321 ggcggtacag gattcgaacc tgtgacctct tccgtgtgaa ggaagcgctc taaccaactg
     1381 agactaaccg cccatctcaa agatcccgat ccatctgcct atctacgcaa acaggcaggt
     1441 aaaaaacaaa ccaactatcc gccatcgttc tcgcttaagc cgcacaggcg caagcgagaa
     1501 tgaacccgga cggatcaggc ccgtccgacg tactccccgg tctctgtatt aatcttgact
     1561 tcgtcccctt cattgacaaa aagagggaca tta
//
LOCUS       DAOSIO010000263         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_67482_length_1590_cov_4.403909, whole genome shotgun sequence.
ACCESSION   DAOSIO010000263 DAOSIO010000000
VERSION     DAOSIO010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67482_length_1590_cov_4.403909"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PK590_07870"
     CDS             complement(<1..707)
                     /locus_tag="PK590_07870"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOG24551.1"
                     /translation="MKTPVSFQLFGIFGYPLTHTLSPGMHEAGFRALGIDANYIVLEL
                     DPAGFRNAMRHIKKSQLSGFNVTVPYKEVVLKFLDQVSPEARAIGAVNTVCKRGNRWV
                     GTNTDMEGFLISLSREGRFCPRNKKAVVLGAGGASRAVTYGLCSRGIKKLSVVDAVPG
                     KAGKIVRDFQKTFKGTECVPLQAGNPEIASLISQADLVVNATPLGLKKGDPLPVPVEW
                     IPTARKRGKVSRGRPVSR"
     gene            complement(704..>1590)
                     /locus_tag="PK590_07875"
     CDS             complement(704..>1590)
                     /locus_tag="PK590_07875"
                     /inference="COORDINATES: protein motif:HMM:NF020091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOG24552.1"
                     /translation="ILAGANQVECTVNAIGERAGNASMEEIVMTIDTRKDLMNCYTDI
                     KLSEITKTSRLVSHLTGMVVQPNRAIVGRNAFWHASGIHQDGFLKKRQTYEIMNPKKI
                     GLSGSQLMLGKLSGKHAFAVRIKKLGFHLSPKEMDDAFAKFKTLADKKKYIFDEDLEA
                     ILKNQMLQIPEVWKLVSMKVVLETGQKSSATVRLSHHGQVSESSSTGDGPVDACYRAI
                     EQVVQSKAQVAHYGLQSVTGGKDALGEVIVKLVVGSKEVTGRGTSTDIIEASVHAYLF
                     ALNKLSQEPKLVQTPSPV"
BASE COUNT          368 a          446 c          375 g          401 t
ORIGIN      
        1 cgcgacactg gccgccctcg cgaaaccttc cctctttttc ttgccgttgg aatccactcc
       61 accggaacgg gcaagggatc cccttttttg agacccagcg gcgttgcgtt aacaaccaga
      121 tccgcttgcg aaatcagaga agcgatctca ggatttcccg cctgaagcgg tacacattct
      181 gttcctttaa aagttttttg aaaatcccgg acgatttttc cggctttgcc tgggaccgca
      241 tccacaaccg acaatttttt tatgcctcgg gaacatagtc cataggtgac tgcccgcgac
      301 gcccctcccg cgccaagaac cactgccttt ttgttgcggg gacaaaaacg tccttcacga
      361 gataacgaga tcaaaaatcc ttccatgtca gtgttcgtgc caacccagcg gtttcctctt
      421 ttacagactg tattgactgc tccaatggcc cgcgcctcgg gagaaacctg atcaagaaac
      481 ttcagcacga cttccttgta gggcaccgtc acgttaaacc cggacagttg agactttttg
      541 atgtgcctca tggcgttgcg gaaacccgcg gggtccagct caaggacaat gtagttggca
      601 tctatcccaa gcgcgcgaaa tccggcctcg tgcatgccgg gagataatgt gtgagtgaga
      661 ggataaccaa aaattccgaa tagttggaat gagactggcg tcttcacacg ggtgacggtg
      721 tttgaaccag cttgggttcc tgagacaact tgtttaacgc aaaaagatac gcgtgcacgc
      781 tggcttcgat gatatcagtg ctcgtcccac gccccgtaac ttctttagag cccaccacaa
      841 gtttcacgat cacctcgccc aaagcgtctt taccaccggt gacggactgc aagccatagt
      901 gcgcgacctg ggccttcgac tgaacgacct gctcaatcgc cctgtagcac gcatcaacgg
      961 ggccgtcgcc ggtcgagctg gattcactga cctgtccatg atgagaaagg cggacggtgg
     1021 cagaggattt ttgtcccgtt tctagaacca ccttcatcga aacaagcttc catacctccg
     1081 gaatttgaag catctggttt tttaaaatcg cttcgagatc ttcgtcgaaa atatactttt
     1141 tcttgtccgc cagcgtttta aacttggcaa aagcatcatc catttctttc ggggacagat
     1201 gaaatccgag tttttttata cgcaccgcga acgcgtgctt gccggaaagc ttgcccagca
     1261 tcagctggct tccggaaagt ccgatttttt tcgggttcat gatttcgtaa gtctgccttt
     1321 ttttcaggaa accgtcctga tggatccctg aggcatgcca aaaagcattg cgtccgacaa
     1381 tcgccctgtt gggttgaacc accatcccgg taagatgcga caccaaccgg gaagttttgg
     1441 tgatttcaga cagcttgatg tccgtgtaac aattcataag atccttgcgc gtatcgatcg
     1501 tcatcacgat ctcttccatc gaggcattac cggcacgttc tccgatcgcg ttcacggtac
     1561 attccacctg gttagcaccg gccaaaatcg
//
LOCUS       DAOSIO010000264         1566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_68963_length_1566_cov_5.084050, whole genome shotgun sequence.
ACCESSION   DAOSIO010000264 DAOSIO010000000
VERSION     DAOSIO010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68963_length_1566_cov_5.084050"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="PK590_07880"
     CDS             complement(<1..179)
                     /locus_tag="PK590_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019002906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HOG24553.1"
                     /translation="MNFIQTEIEGVVVIEPRIFPDPRGCFFESYRKDFFRQNGIPDDF
                     VQDNNSVSARGVLRGL"
     gene            complement(244..609)
                     /locus_tag="PK590_07885"
     CDS             complement(244..609)
                     /locus_tag="PK590_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG24554.1"
                     /translation="MANIKKILIVDDETGIVEEIRSFLAEEGFEVATADTAKQGIQRM
                     ESMVPDVVFIDVKLPDASGIEVLKAAKQGSPGTKAVMVTGYVDQNLMDEAEAMGCDSF
                     LQKPFDLVRILEEIDRLTA"
     gene            complement(720..>1566)
                     /locus_tag="PK590_07890"
     CDS             complement(720..>1566)
                     /locus_tag="PK590_07890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG24555.1"
                     /translation="EQSRGFLEKVLSEAEGANKQLHDAHAQLIHEQKMATLGRLAASV
                     GHEVNNPLTILSMNVSRAILKYRKDPEIRVAEIMDVFDKMEKNIERIKAVVNTLTGLL
                     KRSEKGKFEPLSLKLILEETLPLVQFQTYLDNLSGTEVDFNVPGNIPLIRGDLERLQE
                     VFLNMFINAYHAMAGKRHRKIMVWAERLPGDQDMVAVHFADNGSGMTEEVRKKIFNYG
                     FTTKPSGKGSGLGLYMCKYIIELHGGTISVKSKVGGGTTFTLTLPVYQGEETAKKISS
                     SGIAG"
BASE COUNT          396 a          469 c          306 g          395 t
ORIGIN      
        1 agaccgcgga gcacgcctct cgcggaaaca ctgttattat cctgcacaaa atcatccggg
       61 ataccattct gacgaaaaaa atccttccgg taactctcaa aaaaacaacc cctcgggtca
      121 ggaaaaatcc tgggctcaat tacaacgacg ccctcaattt ctgtttgaat aaaattcatt
      181 taaactcccc gaattcctga cccagaacga aactcacgtc ttcagcggat tatagcatca
      241 tcctcaggct gtcagccgat cgatctcttc caaaatcctg acaaggtcaa aaggtttttg
      301 caaaaaagaa tcacaaccca tcgcctccgc ctcatccatc agattttggt ccacataccc
      361 cgtcaccatc acagccttgg ttccgggact cccctgtttc gccgccttga gcacttcaat
      421 tcccgaagcg tcaggcagtt tgacatcgat aaacacaaca tccggaacca tggattccat
      481 ccgttgaatt ccctgtttcg cggtgtccgc ggtagccacc tcaaaaccct cctccgcgag
      541 aaaactccgg atttcttcaa caatccccgt ttcatcgtcc acaatcagaa ttttttttat
      601 gttcgccata ttaacccgct tgaatatttt accaacatgt ttcttttaaa aaacatacgc
      661 cgacagcccc gtcaccgtga acagagccag gctcacgcga aaataaaatt ctagccgttt
      721 tatcccgcga ttccagacga agaaatcttt tttgccgttt cctctccttg atacaccggc
      781 aaggtaagtg tgaaggtcgt cccccctcca accttcgact taaccgaaat cgtcccgcca
      841 tgtagctcga taatgtattt gcacatgtaa aggcccagcc cggacccctt cccggaaggt
      901 ttggtcgtaa acccgtagtt aaaaatcttt ttcctgactt cttcggtcat gccgcttccg
      961 ttatccgcga agtgaactgc caccatgtcc tgatccccgg ggagccgttc cgcccaaacc
     1021 atgatcttgc gatgacgctt gcccgccatc gcgtgataag cgttaataaa catgttaaga
     1081 aaaacttcct gcaaccgctc aagatcgccg cggatcagcg ggatgttccc cggcacgtta
     1141 aaatcaactt ccgtccccga caaattatca agataagtct gaaattgcac caggggaagc
     1201 gtttcttcga ggatcagctt gagcgagagc ggctcaaatt ttcctttctc ggaccttttg
     1261 agaagtcccg tgagcgtatt gacaaccgcc ttgatccgct caatattttt ttccatcttg
     1321 tcaaaaacat ccatgatctc ggccacccgg atttccggat ccttccggta ctttaaaatc
     1381 gcccgcgaca cgttcatcga aagaatcgtg agaggattat tcacctcgtg tccaaccgac
     1441 gcggcaagcc gtcccagggt cgccatcttc tgctcgtgaa taagctgggc gtgggcgtca
     1501 tgaagttgct tgttagcgcc ctccgcttcg cttaagacct tctcaaggaa ccctcgggat
     1561 tgctca
//
LOCUS       DAOSIO010000265         1544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_70387_length_1544_cov_3.143049, whole genome shotgun sequence.
ACCESSION   DAOSIO010000265 DAOSIO010000000
VERSION     DAOSIO010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70387_length_1544_cov_3.143049"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PK590_07895"
     CDS             complement(<1..442)
                     /locus_tag="PK590_07895"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG24556.1"
                     /translation="MPALRSNHQKYLTRNPVQIFLIRRFFKTIRGLFQYLPAGPLCEV
                     GCGEGFVLRDLSDHGCLRGISVVGLDIDAEALQLASSRVPAVTFQKTSAYQLPFHDKQ
                     LKCVMMLEVLEHLEYPEKALREATRISDYLILSVPWEPFFRVANF"
     gene            complement(449..1411)
                     /locus_tag="PK590_07900"
     CDS             complement(449..1411)
                     /locus_tag="PK590_07900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG24557.1"
                     /translation="MMEEAPLFSVVIPLLNESANIIPLLKEISDVMSRQAWTYEVLLI
                     DDGSTDATPQILKKLIREDPHVRAFIFRAHRGKSAALACGFQNARGSYVVTLDGDLQD
                     SPEEITILWKVMKEENADLVSGWKKKRNDPWHKIVSSRIFNAFARGITGLRLHDFNCG
                     LKLYRAVVIRDLPLYGELHQFIPALVSWRGFRVTEAPVIHRPRKFGKSKYGIERMFGM
                     VVDLLTVAFLMRYEGKPSHLFSGFGLVFLAVGVLINAHLLLTKLLGGSIAPHYPYMVL
                     GVTLLLIGLQCVFFGLLAEMVLYFSRDRVSRGFCHSFPPEEPGR"
BASE COUNT          438 a          410 c          359 g          337 t
ORIGIN      
        1 gaaaatttgc cactctaaaa aaaggttccc agggaacgct caggatgaga tagtcactga
       61 ttcgcgtagc ttcacgaaga gccttttcgg gatattctag atgttccaaa acctcaagca
      121 tcatgacaca tttcaattgc ttgtcgtgaa aaggtaactg gtaagcagag gtcttttgaa
      181 atgtaacggc cggaacccgg ctggacgcga gctgaagagc ctccgcatca atatccagac
      241 ccacgacgga aattccccgc agacatccat gatccgataa atcacgcaac acaaatcctt
      301 cgccgcaacc cacttcacaa agggggcccg ccggaagata ttgaaaaaga ccgcgaattg
      361 ttttaaaaaa acgccggatt aaaaaaatct ggacgggatt tcgagtaaga tatttctggt
      421 ggttacttct cagagccggc atgatccttc agcggccggg ttcttctggc gggaacgaat
      481 ggcagaatcc acgacttacc cggtctcggg aaaaatacaa aaccatttcg gcaagtagcc
      541 cgaagaacac acactgaagc ccgatgagaa gaagggtcac tccgagcacc atgtaggggt
      601 aatggggcgc gattgaaccc cccagcaact ttgtcaacaa gaggtgcgcg ttaatgagca
      661 cccccaccgc gagaaaaaca agtccgaacc cgctgaacaa atgggaaggc ttcccttcat
      721 agcgcatcaa aaacgcgact gtcaaaagat ccaccaccat cccgaacatc cgttcaatgc
      781 cgtactttga ttttccgaat tttcgaggac ggtgaatgac gggcgcttca gtaacccgaa
      841 accctctcca tgaaacaagc gccggaatga actggtggag ctctccatac aggggaagat
      901 cgcgaatcac aaccgcccga tacaatttca agccacagtt aaaatcgtga agacgaagtc
      961 cggtaatccc tctcgcaaat gcgttaaaga tccgggacga aacgatcttg tgccaggggt
     1021 cattgcgttt tttcttccag ccggaaacaa gatccgcgtt ttcttctttc atcactttcc
     1081 agaggatcgt gatttcttca ggtgaatcct ggagatcccc gtccaacgtt accacatagc
     1141 tcccgcgagc attctgaaac ccgcaagcaa gcgcggccga tttgccgcga tgggcccgaa
     1201 aaataaacgc tcggacatga gggtcttccc ggatcagttt tttcaaaatc tggggcgtcg
     1261 cgtcagtact accgtcatca atcagcagga cttcgtaggt ccaagcctgc cggctcataa
     1321 cgtcacttat ttcctttagt agcggaatta tattggccga ttcgttcaga agggggatca
     1381 ctacagaaaa cagaggtgct tcttccatca ttgccaggct ccatcgcccg caaccactac
     1441 tttaggcggc tgaaacatac ccctgtttct tcagaaagga atccatcttg gacacaaggt
     1501 cctcgctgtc tttatagaca aggagctgag aatgttgaaa tacc
//
LOCUS       DAOSIO010000266         1532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_1699
            NODE_71205_length_1532_cov_3.373731, whole genome shotgun sequence.
ACCESSION   DAOSIO010000266 DAOSIO010000000
VERSION     DAOSIO010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863228
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,582
            CDSs (total)                      :: 1,554
            Genes (coding)                    :: 1,548
            CDSs (with protein)               :: 1,548
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71205_length_1532_cov_3.373731"
                     /isolate="AMR_MDS_1699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1058)
                     /locus_tag="PK590_07905"
     CDS             complement(114..1058)
                     /locus_tag="PK590_07905"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HOG24558.1"
                     /translation="MKNKNYDKKIFRLVSILNKLNGGGKVRSSQLAEEFNVSIRSVQR
                     DLDLLNMTGFPLDMQEKGVYSFMSGFSLRKLMLTDEEASLLSFFHEIANSLGKNFSES
                     FRSLVSKVLAKEDSSPFFVKLPGIPAHMKNFPFVKELEQAVQESQKILIKYESAKGLR
                     EHQLCPLKLVNFEGFWYLLAMPEGKRQFRKFRSEKIKALEVLDETFNPPRNLKTALDR
                     SVNIWFSEEPGEKILLEIDKDAAHYFKKKIYFPEQRIMKEKKDGTLILQTQANHNEVL
                     RTIIQWIPDIRVIKPKELDLKVRQLLNAYLKGSRPRWG"
     gene            complement(1079..>1532)
                     /locus_tag="PK590_07910"
     CDS             complement(1079..>1532)
                     /locus_tag="PK590_07910"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HOG24559.1"
                     /translation="DANGYREDKINEGLFDIQMYGFTQYEKRDVIKKAMAIKDAFIEV
                     MTQDRAFIESIERGTYDTQRVKLRTEKWLQRLRDIIGYPENDRRLYTFEEKKALYEAG
                     KEVCYHCGGKIAFIEDAHVDHLERFSEGGKTTIKNGKISHRYCNLQKG"
BASE COUNT          375 a          383 c          335 g          439 t
ORIGIN      
        1 gttgtaaaat ctacaaaaag gagtgtagcc gaagacagcg acagcattat gtcgcctata
       61 tttcgtaggg atactttttt gaattactag gggtagaacg gggtaaaaac cgattacccc
      121 cagcgggggc gggagccttt aaggtaggcg ttcaggagct gccggacctt aaggtcgagc
      181 tcctttggct tgatcactcg tatatccgga atccattgaa taatggtgcg cagaacctcg
      241 ttgtggtttg cctgggtctg aaggattaaa gtgccgtcct ttttctcttt cataatccgc
      301 tgctctggaa aataaatctt tttcttaaaa tagtgggcag cgtccttgtc gatttccagc
      361 aggatcttct ccccaggctc ttcgctgaac cagatattca cgctccgatc gagcgccgtt
      421 ttcagattcc gcggaggatt gaaagtctcg tccagaacct cgagcgcctt gatcttctca
      481 gaccggaatt tccgaaactg ccgctttcct tctggcatgg caagcaggta ccagaaccct
      541 tcaaagttga ccagtttgag cggacaaagc tggtgttccc gaagtccctt tgcggattcg
      601 tatttgatca ggatcttctg cgactcttgg accgcttgtt ccagctcctt gacgaatgga
      661 aaattcttca tatgcgcggg gatcccgggc aatttcacaa aaaacggcga ggaatcttct
      721 ttcgcaagaa ccttgctcac aagactacgg aaagattcag agaagttttt tccgagcgaa
      781 ttcgcgattt catgaaaaaa actgagaagc gacgcctcct catcggtaag catgagtttc
      841 cgaagagaaa atccggacat gaatgaataa acgccctttt cctgcatgtc gagcggaaac
      901 cccgtcatgt ttaaaagatc caagtcgcgc tggacgcttc gaatcgaaac gttaaattcc
      961 tccgccagct gactcgagcg caccttcccg cccccgttga gcttattcaa aatcgacact
     1021 agccggaaaa tctttttgtc gtagttttta tttttcatga aacagaattc cctaaatatt
     1081 accccttttg cagattgcaa taacgatgac ttatcttgcc gttcttgata gtggtcttgc
     1141 cgccttcaga aaacctctcc agatggtcaa cgtgcgcatc ttctatgaag gcgatcttcc
     1201 caccgcagtg gtaacaaact tctttgccag cttcatacaa tgctttcttt tcttcgaagg
     1261 tatagagcct acgatcgttc tcagggtagc caataatatc tcgcaggcgt tgcaaccatt
     1321 tttccgtccg cagcttgact ctttgtgtgt catacgtgcc acgctcgatg gattcaataa
     1381 aggccctgtc ttgggtcatc acttcgataa acgcatcctt tatcgccatg gcttttttga
     1441 ttacatctct tttttcatac tgggtgaagc catacatttg gatatcaaac agaccttcgt
     1501 taatcttatc ctcgcgatat ccattggcat ct
//
